LOCUS       DAONQZ010000001       118265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_107_length_118265_cov_15.357939, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000001 DAONQZ010000000
VERSION     DAONQZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 118265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 118265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118265
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_118265_cov_15.357939"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="PLR13_00005"
     CDS             <1..1955
                     /locus_tag="PLR13_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQC17737.1"
                     /translation="ANIYLPRGSACLDSKPLYNSRIVNSYVKYIKKCYEYINIDELYD
                     YAGIEPYEVADQNHWFSQEQINRFHEKLDELTGASNLAREAGRYAASPESIGVMRQYV
                     LGMANPGTVYKMIGKVTSQFSRSSIYESREINPQKIEITVTPKEGVQEQPFQCENRMG
                     HFESISLVFNNAIPEIEHPECMFKGGDCCRYIISWGNNRSYLLKRTRNIIAIVMAIVA
                     IILTIIYPVDELVYFIPIIIITILIINFLNDKQTKKELRDSILNLEHSRDDLIQQMEI
                     NYNNSQMVHEIGQAINKYININEILDNVIQVLEKRLGYDRCVILFSNEDATRLVFKAG
                     YGYTEKQMAIVKSTEFDLTKPQSKGVFVTSFREQKPYLINDISTITGKLSQKSLQFVK
                     DMGAQSFICCPILSDTKSVGILTVDNFKTQKTLVESDLRLLMGIASVLGVSIRNAELH
                     EQRNRQLKSILKALAASIDARDPYTAGHSEKVTEYAVGICKEMNMPSDYTEVVAVAAS
                     LHDYGKIGISDALLKKEGLLTAEEYETVKTHSVKTRKILEKIEFHGPYSLVPEIAEAH
                     HEKLDGSGYPRGLKGHEIPIGSRIIAVADFFEAITAKRLYRDPIPVDEAFEMLRRESD
                     VHFSREIVEAFIKFYTSNNMASQKSM"
     gene            2031..2843
                     /locus_tag="PLR13_00010"
     CDS             2031..2843
                     /locus_tag="PLR13_00010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC17738.1"
                     /translation="MTQTQTYLMENSDEEKRLDIKTDPDAVKEQALWFGMGPGARVLD
                     VGCGPGKTTALLHDVVQPDGEAVGVDIAESRILYARSFYGYRKGIKFDVRDVRQPMAD
                     LGRFDFIWVRFVLEYYREGAFDIIKNISANLKPGGYMCLLDLDHNCLCHWEMPREMED
                     VVIKAMLHMQNCFNFDLYAGRKCYAHLYDLGFKDLTLNVVAHHLIYGKLQHSDEFNWM
                     KKLEIGALKAPEIFESYAGGSKKFLDDFRIFFRDPRRFTYSPMILCRGRKPL"
     gene            2935..5340
                     /locus_tag="PLR13_00015"
     CDS             2935..5340
                     /locus_tag="PLR13_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17739.1"
                     /translation="MMKFVQNFRHDWQQNIIKRPPPRRIVSILKKAWQLEEYYAGIFE
                     KNNYAVRGNAGISTADAEYSAILPSFQLDVTEDANRAEESYDSRKFRFKYNLPFKKID
                     PRVVSSALATSPKVNKKILINLINHLHFTKEQVSIHIRDLNTGDEILQQGEPGPYLND
                     TVTIRFIDPGAFDLERHHPLNLIIDNRKSLIVFPIDLQSVNHESIVFKMPDVAHSYSK
                     RQAVRHTCEGVDAEIVQGTLNVHGVLEDINPHDLRVCIRAPEDINPANHLFLKLSQSD
                     QTHFMGECQMLRSDNEGSYIILQPVHQNRPVLSPRKYAADRVRITPPPVINFIHPLCG
                     LAVQNKVDDISASGFSVTVSDNESLLIPGMLLPQMTLHLPGMFNDLVCAAQVVYRLKL
                     KKNMVKYGFYILDMSLNDQKRLFDAVSKAADPHVNLTGRVNMDSLWELFFDSGFIYPD
                     KYGIISPYTEALKETYRRLYEEGQDIFTHLTYQDQGQVYGHLSMVKAYENSWIIHHLA
                     ARPLKGKRTGLKTLNYFINYLDCLFRLPVSSKTMRYTFCYYRPENTFSDYYFGGVCKI
                     FKRREICSMDLFGYMPMTVDAEKTGLPSDWSLENFSRDDLALMREYYSGIGGGMMLDA
                     YALECRAAETELSGQVHEVLTENKNNIMDMYNKIGLKRESRVLSVKHDGRIKAALIVD
                     TSDLGVNMSELLNSIKIIVIDHTLPWPVLQDAVSIAGKIYNSDSIMVLIFPYAYLGQQ
                     GVECKKRYNLWVLNSNLDSPEMDIIKDRAKITKRKFITEKFIKEIARNRLKRLLALIQ
                     KQQ"
     gene            5405..6238
                     /locus_tag="PLR13_00020"
     CDS             5405..6238
                     /locus_tag="PLR13_00020"
                     /inference="COORDINATES: protein motif:HMM:NF005881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC17740.1"
                     /translation="MKMLSNKNVLITGAASGIGRATAAAMGLAGCRLFLTDINAEGLK
                     KTADMISSAGDNVCLSRPFDVGDYEAMRAFASEIHAEFGSLDILVNVAGVALFAKIED
                     MQHRDWEKIIRINLWGVIHGLECFVPEMIRAGKGGHVVSVSSTAGLIGLPWHVAYAAT
                     KHALVGMSEVLRYDLRKYKIGVSVVCPGAVHTGLVNTADIRTDDHEATNKARKMFVKI
                     AMTPEKVADLIIQAILKNKYLVITSLDVKLFYFIKRTCFPLYHLVMLIINRFMDKALK
                     K"
     gene            6265..6969
                     /gene="radC"
                     /locus_tag="PLR13_00025"
     CDS             6265..6969
                     /gene="radC"
                     /locus_tag="PLR13_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQC17741.1"
                     /translation="MIQSESKHSKGINSWPEDDRPREKLLRQGAETLSNSELLAILLR
                     TGVKGASAIDLARRIVEKFGTFRNMIHTDVREWKEFKGLGPAKMAQIQAALEIGRRFR
                     EVEALKGKQKIASCCDVVEIVMPQMRDRKTELFKVLYLNSNNKIIDISDVAEGTVNHA
                     IPIVREIIHAALQKFAAAIICVHNHPSANISPSAEDRKFTQDLLAAGKVMGIKVLDHI
                     IIGDGNYYSFAEEGIL"
     gene            7057..8274
                     /locus_tag="PLR13_00030"
     CDS             7057..8274
                     /locus_tag="PLR13_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQC17742.1"
                     /translation="MTYYIIIAFCVVILLAYFFDVTSRQTKIPGVLLLILTGMAINSI
                     DSYFNFEVSQIDVILPVMGTVGLILIVLEASLDLTLSKQKKSLILKSMSSAIVLFLVF
                     TGLFALMLSYGLGYDVRTSLVNIIPVAVISSAVAIPSAVGLSGNNREFVIYESSISDI
                     FGILAFNYVVISNGHLFSGILNFLLEIIVTLFASVLFSAFLAVILHKINHHVKYIIIM
                     TAIILVYALSRLLHLPSLLVVLIFGLVMNNNNLFENSITAQFINFQDFNTELNAFKNI
                     TSEMTFIVRSFFFVLFGYYTSLFDLLNLNNLLFSVLIVAAIYALRALYLKSIIRISMT
                     PLLYFAPRGLITILLFLSIPQAMALPYMNTGVVTQIIFITILIMAYGNIVFGKTGEAI
                     PTGTPQDKKNLSG"
     gene            8534..8851
                     /locus_tag="PLR13_00035"
     CDS             8534..8851
                     /locus_tag="PLR13_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17743.1"
                     /translation="MIIRKSAVVAVMLIALFLLSCGMSDPFSGKWFNDVIEKEMLTIT
                     KSGSAYKVDTALGSFPCIKEGDLLKCQMGIGDTMLQINENRCLVMKTPAGVEFKFRRQ
                     ENK"
     gene            8885..9952
                     /locus_tag="PLR13_00040"
     CDS             8885..9952
                     /locus_tag="PLR13_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQC17744.1"
                     /translation="MIFENVALHNVDGLYKHKDIEGLLLGRIPEHVSARLSKAGQMMM
                     ICPSGSEIRFVSETYPVKITLSVDKITKHLGDGIITDARVFFGTFQTRQRFVIKRIKT
                     LLEIIRPPKFIELAEKIATDAPFSPHVCRIRFWGTTMGAPIRFHGIETEGKIRPPHAE
                     ELPGLSYLAYGTSLTQGAYASESHLSYPNLVGCRLGVDVINLGSSCSAYCEPEMADYI
                     AGRKDWSFATLDLSLNMVDTFSPEEFSRRVSYMINRIAGSNKSRPVFCITLKPYLGDY
                     IESKKPDLYRKLLRKAVENSALDNLYLIEGQDLMPDVAGGLCPDMVHLGDFGTIQIAE
                     KLAAKIKPVLQSHHLLFKHSR"
     gene            10135..11127
                     /locus_tag="PLR13_00045"
     CDS             10135..11127
                     /locus_tag="PLR13_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQC17745.1"
                     /translation="MYDHDTSKFQSVKEFIKHNYRHFNSAVVVDAAEAYANHINNGGK
                     MLMALAGAMSTAELGITLAAMIRSGKIHAVCCTGANLEEDLFNLVAHKHYKRVPNYRD
                     LKPEDEAELLRNKLNRVTDTCIPEEEAMRKVEGKLFSFWKKAADENKRYFPYEFIMQI
                     ISSGVLKDQYEIDEKDSWLVAACEKNIPIFVPGWEDSSLGNYFVSSVRRKQLKHYSIV
                     KSGLETMDALIDWYLDASRHSETGFFQIGGGIAGDFSICVVPLIQQDMKMDCRLWSYF
                     CQISDSTTSYGSYSGAVPNEKITWGKIRPDSKKFIIESDATIVAPLIFAYVLAE"
     gene            complement(11155..12198)
                     /locus_tag="PLR13_00050"
     CDS             complement(11155..12198)
                     /locus_tag="PLR13_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQC17746.1"
                     /translation="MKDLFFEPVKGGKKPYLESLRKLLQHLRPPFSAGDTVGIKLHWG
                     EKGNKGFLPPDYAREIATWLIAHGTKPFVFDTTVLYSGGRRTAEDSLITAASHGYTHD
                     FLGCPVIIADGMDSRNLVDIPAGFNHFRTVQVANVFENAQGFVIFSHFKGHMVSLFGG
                     AIKNMSMGFASRAQKQRMHSDYHPVLNKKKCTRCGVCAEICPTGAATIATSDYPTYDL
                     KKCIGCAQCIAMCPEMALQIFWNSDMTVFQEKLVETAAAVWKIIKDKTIVINSLLNIT
                     VECDCLPGKNPVIHEDMGFIGGYHPVPVDAESLKMTGADIFEKVHPGIPWQRQFTYAR
                     EIGFSPARNITGF"
     assembly_gap    12245..12344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12345..12777
                     /locus_tag="PLR13_00055"
     CDS             <12345..12777
                     /locus_tag="PLR13_00055"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HQC17747.1"
                     /translation="HQQTIAKTVMLIPDFSKYSLALFEGGALIHASNGKGVRPLFEAL
                     EKFRAKSGLILHDKIIGLAAAKLVVYSGIIAKVNTTVASAPADRFLKDHGILMNAAGI
                     TANILTQDKRAVCPGEVIALKTKDPADFIRQIKTMLDNQRK"
     gene            13107..14078
                     /locus_tag="PLR13_00060"
     CDS             13107..14078
                     /locus_tag="PLR13_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC17748.1"
                     /translation="MKICPVCQTRFKDKTQKCRYCNVVLEPLTPASASQPPQPDQQAK
                     QPKIVKDSRKKRQGKAPIERQTGFGKTWNRNKYPFIVVIILIVGILLAIVQKCSEKKV
                     EVSNTQAVEQQAVYAPVNQPAASDNRMIAAAPARDEANGLFRKAFELCISGKCTDPRK
                     AIEYLSEAIKLKPDYADAYNNRGNAYIEIGQYPEAVQDYNESIKLQPQSIYGYNNRGL
                     ALSKLGKHQEAIADYNEAIRLKPDSAIIHNNRGAAYLAIGQYQKAVEDFSEAVKIKPD
                     YVDAYYNRANVYFDHMNKEDGCNDAQKACELGKCDLLEKVKGKGYCS"
     gene            complement(14553..15083)
                     /locus_tag="PLR13_00065"
     CDS             complement(14553..15083)
                     /locus_tag="PLR13_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQC17749.1"
                     /translation="MARIPDFQPGPADTEKKFLQGKPPFDLNIFKIITHAPVGIAREF
                     IGLPASVLMAGKLDPVLREMAITRAGILCNSAYEVHQHRKLSKLVGLPEEKIKALDTG
                     SASPVFSGIEKLVLRFTEETVLQREVSDATFAEISRHFSEDLLVELSIAVCCYMAVSI
                     FLNTFDVNLEENDVPF"
     gene            complement(15096..16487)
                     /locus_tag="PLR13_00070"
     CDS             complement(15096..16487)
                     /locus_tag="PLR13_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQC17750.1"
                     /translation="MLDYFFNPKSVAIIGASSNMNKGGYHIIKNITSGFPGKIYPVNK
                     NYPDMLGLPCYKDLASIPGNIDLAIYFIPSKELPDTVNECAKKGVKGIIIESGGFNEA
                     GAEGARIQKLALDNAARAGIRLWGPNCMGFIDANKRYVFSFINSLVWPDVLRGGNVGM
                     IVQSGMLSAGFLLHALQEGIMGVSKVCSIGNKCDINENDILEHLIDDKDTEVIACYLE
                     SLVDGRKFLDLAKKTEKPIIVLMGGRSQEGAKAAQSHTASLSGNYKIASAAFRQAGII
                     EVFDPAELTDIARAFAKNMPFHPEKEKGTAILTFSGGAGTVTTDLMEDCGLKLAKLSD
                     RTLAAVAELFPPWNKPDHPLDLWIAIERHGFEKVFRNSLTAVINDPGVDSIIFHSYAS
                     PLIGQDFFEELAAMIKKNKKPAVLWIEGRKDFAEHIRGIAENAGLPAYREMNRCVTVM
                     NGMKRHFTKKPMS"
     gene            complement(16619..17287)
                     /locus_tag="PLR13_00075"
     CDS             complement(16619..17287)
                     /locus_tag="PLR13_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC17751.1"
                     /translation="MLDVIEKALKEGRKTLSEYESRQVIESAGVAVAVAALTKTKEEA
                     IREAEQMGYPVAMKGCSAELSHKTEAGMVALNVNSAQDVAQVFDALTGKVKNLDGVLV
                     EKMVRGSRELVIGLSQDSSFGPCVMFGLGGIFTEALKDVTFRVAPLTKQDALEMIDEI
                     KTKKLLGEFRGSPAVDRNALAKALVGIGDLAIKYEAIAEIDINPLIVCGDKPVAVDAL
                     VVLK"
     gene            17521..19068
                     /gene="putP"
                     /locus_tag="PLR13_00080"
     CDS             17521..19068
                     /gene="putP"
                     /locus_tag="PLR13_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HQC17752.1"
                     /translation="MTGNFNFYMLMSIAAYMVVVIVIGFVFARRNDQIGDFYLGGRKL
                     GTIVTAMSAEASDMSSWLLMGLPGVAYLSGIADAGWTAIGLAIGTYVNWLTVARRLRC
                     YSQLSQNAITLPEFFSNRYRDSKKILTWIASIVILIFFIPYTAAGFASCGKLFNSVFG
                     LDYTTAMLIGAVVVVAYTTVGGFLAASTTDLIQGIVMSIALLSILMFGAAVAGGIDAV
                     WQKADAIPGYLSMMFSHNSTTGQTERYGLITIISTMAWGLGYFGMPHILLRFMAIRDE
                     KKIVFSRQIAVTWVVVSLAVAVLIGIVGQTLSLNGEIAALEGSASETIIIRMAQLLSH
                     TGVISSLIAGVVLSGILAATMSTSDSQLLVASSSVTENLIKECFDIRLSEKTGMMIAR
                     SAVIVIAAIAAFIARDPDSSIFVIISFAWAGFGAAFGPVMLFALFWRRSNVYGAIAGM
                     VSGGVMVFVWKYLVRPLGGVWNIYELLPAFVFACLAIIVVSLLSKAPAREITDEFDAV
                     VKMGNRK"
     gene            19135..19452
                     /locus_tag="PLR13_00085"
     CDS             19135..19452
                     /locus_tag="PLR13_00085"
                     /inference="COORDINATES: protein motif:HMM:NF022815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HQC17753.1"
                     /translation="MKKATKESWTQKVKETSFALDLQSGVFTFDNPRRIAESLKESAE
                     SSTQRKAEPFQSAMSMLNYYINRAGTKLPAERKAILEQAKDELRVLFGRGNKSKTSLK
                     IKK"
     gene            19467..20987
                     /locus_tag="PLR13_00090"
     CDS             19467..20987
                     /locus_tag="PLR13_00090"
                     /inference="COORDINATES: protein motif:HMM:NF036848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HQC17754.1"
                     /translation="MNSENIPVIIGAGQVTKREASSVQGGSPIDLMAAAVKIAAEDGG
                     VTEKYLQQADLLMTTSLFSDDGIANPPGCVADKLNLTDARCRVSGFGGVMPHAVLHHA
                     MQEIAGGKAGLVILTGAEAQHTMQQAKNSKTFTGWNVASGRPCLPPLTPLGLFDGACE
                     TEHIHGLSLPAYVYPMFENALRRRYGCTIEEHAEKIGRLMSRLSLVAAKNPLAWFQHS
                     FTPEEIMTLSETNRNTAFPYTKRMNAMLLVNQAAALLVASEAQAQKMGVNPLKWVYVH
                     GYAEANDHWNVLEREGYGFSPAIEIAGRTALKQAGLTIEDIDFFDLYSCFPVAVQVTR
                     DMLGIPENDSRDLTVTGGLPYFGGPGNNYVMHSMAQMVEVLRRHPGRTGLVTGNSFYM
                     TKHSTAVCSTRSPENNAAATADTRTCQQAVDKRPKYEIDPTPSGRATVDTYTVIYDRD
                     NLPKKGIIIGKEENGKRFAAFTPSDPSLFSAMIEKDFCGVTGRVVSKDKINLFTPD"
     gene            complement(21029..21274)
                     /locus_tag="PLR13_00095"
     CDS             complement(21029..21274)
                     /locus_tag="PLR13_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17755.1"
                     /translation="MNGKYQEIQVECYSGFKANERPVAFTYQGERQEIQEVVDRWYHG
                     GLYSSKPVINFFKVRTADGKVYLLRYQFDSDAWSLRI"
     gene            complement(21305..21763)
                     /locus_tag="PLR13_00100"
     CDS             complement(21305..21763)
                     /locus_tag="PLR13_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQC17756.1"
                     /translation="MPAKKLKEFLDSHGVKYVKLTHSTAYTAHEIAQSLHISGRKVAK
                     TVIVMIDGKMAMAVLPGNRSVNFDHFQKAVGAKKIELATEAQFKGMFPDCELGAMPPF
                     GNLYGMEVYADQHLTDDDEIFFNACSHSELVEMSYPDYERLVKPNVVKLT"
     gene            complement(21978..22247)
                     /locus_tag="PLR13_00105"
     CDS             complement(21978..22247)
                     /locus_tag="PLR13_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC17757.1"
                     /translation="MVFLPEGRYYGSLYRSFLCREINNLLVTGRAISMDRGALASARV
                     MGPCLAEGQAAGTACALALSSGQSIDAVDTGKLREMLAKDGARLK"
     gene            22442..23338
                     /locus_tag="PLR13_00110"
     CDS             22442..23338
                     /locus_tag="PLR13_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HQC17758.1"
                     /translation="MNDNLIFQETPDLINPYILIGYRGWLNAGEMATGSIDYLRRKLD
                     ARKFASIDIRNFYIWQVPGFDPAQVMRPHAVVERGLIKSLNEPANEFYFWKSGKENDL
                     ILFSGFEPNVAWPEFTSAVLNIAKRYQACRIYVLGGVFDQVPHTRETIVYAVLSRPEM
                     KHEFQSFPLLNYTGPCSFSTLLVNHAGREGIEAASIVARVPPYIETFNAKIGCEYLKK
                     VLSRTSLNIDLSDLKKSGDAVNKMMENDFRQNLEALVQLRQLEQMYDESLMQGYGENP
                     EVDIDELMQAMHEMKKDIPKKH"
     gene            23420..24004
                     /locus_tag="PLR13_00115"
     CDS             23420..24004
                     /locus_tag="PLR13_00115"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQC17759.1"
                     /translation="MHIPPVFVYPEAVLFWTALIGSFYLEIKFAGALARNPSNTQDAG
                     TRLLIKIGYRVAFVLALMASLTPWFVITWQRPALAVGTGMLIAGSIFRQYSIRALGKY
                     FTPEVVVKADQPVIEEGPYRWIRHPAYSGAFIMFAGIGMALGNWLSFAVLFLENCFVY
                     SLRVKAEEKALIDTIGGPYRVYMTRTKRFIPFIF"
     gene            24171..25469
                     /locus_tag="PLR13_00120"
     CDS             24171..25469
                     /locus_tag="PLR13_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC17760.1"
                     /translation="MKETGRNQASKEIKVLKNKIKELENLLNTTKVGQTRMSTGLVYR
                     TIFRMSPNTIVVSKLEDGTIYDVSDSFCEKSGFARKQVIGKRASTLGIWLDQDREEIK
                     KRLLRNGRYRNMEVRHQVKGGKMLQCLQSAELITIDDERYIITVVVDITERKMMEERL
                     RENEEKYRQLFDNSPAGIYQIDYQTGRFLKANDVFCKYVGCSQEEVTSLTAFDILTEE
                     SKKRFLERVERLSQNLEVPDSVEFEILNKKGESFYIQLQIRNVCDVKGRVVAADVVAH
                     DITERKQAEDELKSFAEDLEEANITLRVLMNQRDEDKQEIEEKLQTNMRDLVMPYLRK
                     LKRLNLDGRSKNYLDILESNLNEVLSPFMKNLQSSFNHLTPQEMQIVSLIKKGKKTKE
                     IAEMLNASTKTIATHRNNLRKKLNLRNSKINLRSYLLSSG"
     gene            25695..26108
                     /locus_tag="PLR13_00125"
     CDS             25695..26108
                     /locus_tag="PLR13_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17761.1"
                     /translation="MLLTLMIFCHPASPVFGASEWIEFYTGNLGNVAFYKKVVSKDGA
                     VKYVIKDVFSDKGREAFIRDRAKKKLSTVEYEEISNVQSLNEIDCREKKIRHVSIFYF
                     DKDGKLFSSDVIDNPKWVKIPDNPFFNALIKKACQ"
     gene            26489..28168
                     /locus_tag="PLR13_00130"
     CDS             26489..28168
                     /locus_tag="PLR13_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC17762.1"
                     /translation="MMQEKKKKIRWDISIWAAVSAITVLMVILGMMMFMQFQQSETQA
                     QALFIEKGATLIQSFEAGLQDRVDGKNDRFYFQKLLMATAEQPDIDYMIVTDSKGKIL
                     ADSDPSMLDQQYGIDLDTEKIARSKDMHWRQVTNPGGAGTFEIYRGLFPWRQTDAALH
                     HRSLIVFVGFNMDKIEKASQEDIYRTIIRAFILILIGSLAIVSLFLVQTYRLTRASLS
                     RVRVFAEALVRNLPIGLLAVDKDNRIIASNEHARDLFAGDPSNVERQDAGRILPGPLV
                     RLLTKVTMSSGLIEEDVVLSSETKGEQIWEVVVAAFADRDVPEGKILLARNVTAVRGL
                     EKEVAHSRHLNAIGSLAAGVAHEIRNPLSSIKGFAVYFKQRFAGNPDDEETADIMIAE
                     TERLNRVIGQLIEFARPLTLKTEKTALSGLVMQSVKLIEVEARKNGVQVDARVPEDLP
                     AVEIDPDKIKQVLLNIFLNALAAMPDGGRLSVGISQTQAGQEVLISDTGSGIEKNDLP
                     RIYDPYFTSKPAGTGLGLAVVQKIMEAHGAFLNIESQAGAGTKVTLRFAVS"
     gene            28373..29752
                     /locus_tag="PLR13_00135"
     CDS             28373..29752
                     /locus_tag="PLR13_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC17763.1"
                     /translation="MKTRLDILITDDDRAHRVMLKKLLNGWGYNVTEADDGLVAVEKV
                     RKSSFDLILMDIRMTNVSGIEALEQIKQINADIPVIIMTAYASVETAVQTLKKGAYDY
                     LTKPLDFDELQVSIARATEHSRLKKENEYLKEKLGEKFDRRNMIGQSPSMIKLLEVVE
                     QVAATQATVLITGESGTGKEVIANAIHFNSARKNAPFVKINCAALTETLLESELFGHE
                     KGAFTGADKRREGKFVQADGGSIFLDEVSEMSPAMQVKLLRVLQERELTRVGGHDVLK
                     IDVRVIAASNKDLKKEIRDGRFREDLFYRLNVVTLDVPPLRERSEDIPLMAHAFLKMF
                     AENNAKVIKGFTPQAMQKLASYSWPGNVRELMNAVERAVVLCRTETIGEEDLIFTMAD
                     QALSREVLSAGQKMPAASGNRSLEEIEKQSILEALQSCRGNKSEAARRLGITRKTLRK
                     RLHKYEDGE"
     gene            complement(29876..30934)
                     /locus_tag="PLR13_00140"
     CDS             complement(29876..30934)
                     /locus_tag="PLR13_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQC17764.1"
                     /translation="MNQQSRADSIHLISSPTLPYFRSVNGIAILARYVEKYGIHVNTL
                     LTGSGLHPRDLDDPEMFVTPEQELAVMRKILFLVPDPKIGLIIGQQYHVGVQGKLGAA
                     AIFSDTGLEAITMFFKYIALTLTYFQYELHVKDKLAFVKVRELIDLKDLRVFVCEREF
                     VSIYRIVSDILKAPIPVNEIRFAYPKPSYASFYEGVFHCPVKFNAPEHRIIFDSTLLS
                     MPLPMSNPIARKTYEQECKQMCQRLKVKETITDQIRQEILFKEESVPSFTQLSRHLNL
                     SPRTLRRRLTDEKTSYKSLVADIRKKKALDWLKTSDCPLETIATRLGYSDISNFYRAF
                     KRWTGRNPGFYRKEHGRI"
     gene            31072..31431
                     /locus_tag="PLR13_00145"
     CDS             31072..31431
                     /locus_tag="PLR13_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17765.1"
                     /translation="MRFSALLFILYQILKWAAKMKPVYRSYVGSIHQVRIMIKTADGK
                     RGRLFIFDRGTVSSKGGADHPYDAAIVWSDAATGFRVMASKSNEEQFKAAAAGKMKLD
                     GMAFFAQWFNDGVKLVM"
     gene            31544..33211
                     /locus_tag="PLR13_00150"
     CDS             31544..33211
                     /locus_tag="PLR13_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQC17766.1"
                     /translation="MAKIMGGHLVGKYLKEVENIDIVFGISGGHIEAVLDSFTEYKIR
                     TIDVRHEQAAAMMAHAWSVYTGKPGVCFLTAGPGFANGLTGIANAYLDNAPLVVLCGR
                     HPLRDDLKGALQEINQMDIVHPITKWAATCYDPKRIPEYLSIAFRYAVEGRPGPVFLE
                     LPPDILGVKIEESLAPMPRRTTRKYTVHPDDADLKAAAQIINNAKNPLFIGGSGVRFS
                     GKSDALKAFIEKTGIPFMLMNYGRGELPDSHSHSVMDMGSVGLMFGLPQTDVLIAAGL
                     RFNWLLMSGSLIPAATKVVRIDIDPHEIDRNRMADAGLVGDVGSVLEQLTPLADKKDH
                     SAWSETLKAAGRSLLDYELRIRETATVPIHPVRLVAQIQKVVGDDTVFVTDGGDTNYF
                     GLAFETRQKAGVIAAAAGLFGCLGTGIPFGMAAKLARPDKKVVVLNGDGSFGFNAMEF
                     DTMVRHNIPVVVVINNDCAWGMIKHSQELSIGADRLQCAELGVRHYEKVVEGLGGHGE
                     FVTKDEEIIPALERAFASGKPACVNVMTDPTVTSPATPLFYQALKME"
     gene            33216..34430
                     /locus_tag="PLR13_00155"
     CDS             33216..34430
                     /locus_tag="PLR13_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQC17767.1"
                     /translation="MKALLLNLDPLRFVALHALRPLSKKFCYQGPFSTVKLVDIPEPV
                     LPSPEWVKIKTRLCGVCGSDINLMFMKDSPSAMPFTSFPCVPGHEFCGDVVDVGADVK
                     KIKKGDLITAIPALNCETRGIKPVCKSCASGTTANCDNFAEGAFAPGMFTGICKDING
                     GFAEYVIVHQSQARVVPAGVSPESAALTEPLAVGLQAVMDNRPADKDKVLVIGGGVIG
                     AMVVKSIRALDCTCDITVVEPSTFAADYIKQCGANRTMKGGIIEAAVKITKGRAYRPM
                     LGERMVMGGFDKVFDTVGNARTLNLSLRVTATNGTLSVIGIGQEVKLDLTTLWLKLQT
                     VKGCYGYRYNSVASSRKHTFDIALELIASRKIQVDDMLTHQLPLENYREMIEINLQKE
                     TRKAMKTAFCFM"
     gene            34545..35156
                     /locus_tag="PLR13_00160"
     CDS             34545..35156
                     /locus_tag="PLR13_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQC17768.1"
                     /translation="MIYFDDLLQDDNICDAVSRIAGEFREKHNFPEVRQLGVVVPDVE
                     TAATQLEKRGIGPFFIASDTLKFWNERGEDRTFSGKLGMAIYKGYEVELLEPGIGSAF
                     YKSCVDGKGRMVLQHLGFLVNDVDDYRNKLEKAGCPTWIRGQIKSFPVVIDFVYMDSV
                     AQAGIILEFIDMRFLGIGVKPPGIIYHGLGKLEKITGIRCLKF"
     gene            35305..35478
                     /locus_tag="PLR13_00165"
     CDS             35305..35478
                     /locus_tag="PLR13_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17769.1"
                     /translation="MLKISKICFAVSGLLLIVDSTLMILNKPNPLGLPLPCPVTLTIL
                     GVGLILFSIAKIK"
     gene            complement(35641..37662)
                     /locus_tag="PLR13_00170"
     CDS             complement(35641..37662)
                     /locus_tag="PLR13_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC17770.1"
                     /translation="MIHPVAFLDIIVILAALAAMAGLARRANDYFLISTRVLLACLSF
                     FLLLYGICLAVEWSGISSRFDRIEDYIGALLPMSWAFVFYSFLNELSFRNIQDLNTQL
                     KQRVDLLKEAELRFRTIFDSASDGILIADVREKTFFAANKKLCDMLGYTKEELQKLGL
                     PDIHPEASLALVNEQFDKLSAGEISVAQNIPLLKKDQDVFFSDVSASLMTFDKKKFLI
                     GIFRDITERRKAEEKLLKSEKKFSSIFHLNPNPLAITDVDTGTFVDINQAFVNWTGYS
                     REEIIGFSAHDLHLWVNLKDREKIVNIVTQTGEASSVEIMMRQKSGQIRTVLFSARFI
                     EIEQTRYLLTLAHDISERKQAEEQYRNIFENAQEGIYRATPEGRFILANRSMARILGY
                     DSQEDLITEITDMASQIYLEPQERTKLIQVMEQKGSIKNNEIQFRRKDDRPIWVSQTM
                     QAVRNEKGQIIYYEGIIEDITERKENVERLRRALGGTVKAIATLVETRDPYTAGHQRQ
                     VSDLARAIATEMGLPNDRIEGLRVAATIHDIGKVSIPAEILSKPSKLTNLEFDLIKTH
                     SQSGYDILQDIEFPWPVAQIVCEHHERINGSGYPNHLKGDEILLESRILSVADVVEAI
                     ASHRPYRASLGIDAALAEIEKNKGVLYDAGVVDACLRLFRQKDYQLTER"
     gene            complement(37659..37916)
                     /locus_tag="PLR13_00175"
     CDS             complement(37659..37916)
                     /locus_tag="PLR13_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17771.1"
                     /translation="MIMIVIGLTLLVFLVLNYRQLKQTPFFQLLFVSYCLSTTGWMMT
                     VLEGFFFEASLNFIEHLCYASSAVLFAVWCWMIPGRTRGNT"
     gene            38336..>38617
                     /locus_tag="PLR13_00180"
     CDS             38336..>38617
                     /locus_tag="PLR13_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC17772.1"
                     /translation="MFDYMMTKEQLKIRDEVRDFIKWVPRQMVLDMDTDKIKFPKEFL
                     QEAARRNLMGCRYPKKWGGRDMDWVTTCMVMEEVGTIGYEMACVFGVGAE"
     assembly_gap    38618..38717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38718..39386
                     /locus_tag="PLR13_00185"
     CDS             <38718..39386
                     /locus_tag="PLR13_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQC17773.1"
                     /translation="YGLMGCRGGGTGRIIFRNVKVPKKNIVGKLQGAYAVFNTMMIPE
                     RLGTAAMTIGAARPALEIATNYTSRRKAFGQIIAQFEGVSFQIAEATMLLDAARAMAY
                     VTAKAVDAGADMHRIRRMVSQSKKFITESCQKAVHNSMQVMGGIGYTNVFPIERIYRD
                     IRLASIWTGTSEVMSMISAHEWYREYLMQKAGQKTREHELDAEEAHEEEKIYDDEDMW
                     KKGW"
     gene            complement(39436..41061)
                     /locus_tag="PLR13_00190"
     CDS             complement(39436..41061)
                     /locus_tag="PLR13_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQC17774.1"
                     /translation="MLQYYWLSQRSRMDNESLLSFGRRDFLKYALLAAQALTLPSLHG
                     CAIPKKNVPAIDDATFLIKNANLVDVTSGLTVENSWLLVENGTIAAMGTGNIKNNYRG
                     MVFDLQNRFLIPGLIDAHCHATASPVFSMRMTDLPNHARQQKQNFVSAVESGITTIRD
                     MGAFPVLLKRFIRDIEKGNMPGPRVVYCNSILNVMGSHPEIPPSDINFFALPASLFIG
                     MIMNNFKNTKEMEECLEENARGASFIKLTLDNRTIFCKKNKAIPVYSKEQLDIIFNFA
                     GKNGLPVVGHHHFKFGFDRAMAYPFHSIEHLIADRILSDDDVLRMAKNNVAIVPTMTV
                     AQSYLMEEAFDNIPPPYATPDVMDELHVRKNYFGHEAFQHCDPVLHKQNLAALKYYKT
                     IGRDNLWEHKKFLVDPDVYFGMMMNGYANLKKMNQAGILIGCGIDAGMPFCYFGGNYR
                     EYEILQRNGFTNIEILRCATISNAKILRLEDKIGSLETGKYADMVVLDKNPLEDIRAL
                     RSPCLVFKQGDPMYSAINLQENGIMGRQPAKNT"
     gene            41246..42247
                     /locus_tag="PLR13_00195"
     CDS             41246..42247
                     /locus_tag="PLR13_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC17775.1"
                     /translation="MRQYKCSVEVLPDVFCIRLPLPGRKPGPVNVYLFKGRKNTLIDT
                     GMAQTAGILKKALAEHHLGFADIDTIIITHGHPEHFGAARKIVLAGRAIVMAHEGDRT
                     FIEKGYGISAKQYKKFFRITGVPRNLEIILRLLFFFFKRMADPCRVDTIVREDDQIDI
                     GRYRAKILETPGHSRGSVSIYLEQEQTLFCGDTVIKHITPNAFVVPDEDATLPVRLSQ
                     DEFFKSLMKIKSLSPRIVYSAHGKPIDDVDKIIAGYEKAFAERQENVLALIHAGEKNI
                     YRIARRLFPNIGGWRLPLEIILSISEVYSAIQILQRKGKVCLRMRNNLLEVDQYRHP"
     gene            42391..43083
                     /locus_tag="PLR13_00200"
     CDS             42391..43083
                     /locus_tag="PLR13_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC17776.1"
                     /translation="MKKIGFLVVMLMLMAVFTTAQAAEVRAGGLPALAADDSNATSTV
                     NLSREGEVRAGGFPKMDKYDTCNDCTPPQCPPPPPPPPPVEEKVCPPCPECPACPPPP
                     VVEAPKPAIIEKGRQTLDVKFAFDKATIKPGYYKDIDALANVMKDYPDLNVVIEGHTD
                     SVGSDAYNKKLSQERADAVKKYMVDNAGINANRLKAMGYGEEKPIASNSTAEGRAQNR
                     RVEAAVDYVIEK"
     gene            complement(43269..43919)
                     /locus_tag="PLR13_00205"
     CDS             complement(43269..43919)
                     /locus_tag="PLR13_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQC17777.1"
                     /translation="MLTALLTIFVSSFIIALSGAMMPGPLLTVTISESSRHGFMTGPL
                     LIAGHALLEILLLAALLLGLAPFFQMPAVFVVTALAGSVILLWMAFNMFRSLPNVRLA
                     WDAHEKSGNHPVINGILMSVANPYWIIWWATIGLGYILYSRQFGLRGIGFFFAGHILA
                     DLSWYSFVSAAVTRGRHFLNDRLYRGILSLCAVFLVVFACSFLYAGIEKLSVILSG"
     gene            complement(43935..44876)
                     /locus_tag="PLR13_00210"
     CDS             complement(43935..44876)
                     /locus_tag="PLR13_00210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQC17778.1"
                     /translation="MYKKIFLAVLLVIVVSSCAPTHASRYREGSNFNQQTNLTIQDEQ
                     RLTREALPQILKDYPSAKNQELQKYISDLGMKIVRANHLEGNPYHYTFSVVDVADINA
                     FALPAGTIFVTVPLIALASNEAELAGVISHEIGHVVARHTAERMYVMEQAQNKSWMYA
                     TGGAVVGAIVGYGLGTVLCSDGDSACKARAALIGGALGAGGGLLAQKYTFLVNSREDE
                     MEADRIGFKYAVAAGYDKDQVGTFYEKLLEIEKKSGQSGNAIAKNLNDALSTHPPSEE
                     RVRQMRELAAASPVRDAVIHTSQFNRAKQIAKKIGKK"
     gene            45057..45902
                     /locus_tag="PLR13_00215"
     CDS             45057..45902
                     /locus_tag="PLR13_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC17779.1"
                     /translation="MPFFKRGNATIYFEDDGWGDPIIAVHGLIENTTYWKTTAEEIIK
                     KYRFIPMDMRGHGKTVVEGEFCGFDAETIGDDIEALADYLNIGEFHLLTHSTGGFAGV
                     RYAMRDSGRLASLILTDTGSSTSVIPGDVETIRKFNDKLACYFEKFNWEQIVANLWAN
                     PFPFFRGIVESPQARDLMRSAQDMISLGDRSDIARFVRSFYTDPDPKIEGLRGIRCPT
                     LIIYGEKDDLFIETSKMMAREIPHAQLLEYPGVGHMTALECPQRLAKDIIEFIKENSR
                     QTFDL"
     gene            46089..46805
                     /locus_tag="PLR13_00220"
     CDS             46089..46805
                     /locus_tag="PLR13_00220"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQC17780.1"
                     /translation="MTRKVFARPVTRSRLHEEIVTVIQQQIMSGQIAPGEKLPTEREM
                     AENFNVNRATVREALRKLENIDLLEIRHGDGLYVKDYLNSGNFDLIKAAVKMDGGTEI
                     IFNILEARRYLVPQMAYLAAQRRTSSDVDDLKQLLSEKNITMLEKDIRIHQQIARCTH
                     NLLCTIGLNFFNQIFRDYGHLYFNDPENVERSRQFHDEIFEAIRRKKADDARNIMRDV
                     LIYAEEAVKNKIKRPTFDKE"
     gene            46811..48457
                     /locus_tag="PLR13_00225"
     CDS             46811..48457
                     /locus_tag="PLR13_00225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HQC17781.1"
                     /translation="MKRFIENYTGEKFDVAVIGGGISGASVAYEAATRGLKVALLEKN
                     DFSWATSAVTSKMIHGGLRYLVNGEVGLVRESLRERRVLENIAPNFVYPWPMMMTHYK
                     KPLKNNKWVVKVGMLVYDVLSYDKNFTWDPCKKIPVHRTISARQVLQDEPHVRAEGLT
                     GASIFYDCTSLFPERLTLAFIKSAVAHGARAANYARVEGFLMDGAKHVLGVKVKDLFS
                     GKMHNVPATVTINCGGPWADIVLGMAKPDGQVNATLRRSEGIHIITSKKLLTGNYTIG
                     SMTPEGRHFFLIPWRNHALIGTTDKPFNGNPDDYRVTKESIMELIADVNSSFGDGKLS
                     YADVKHTYGGLRPLIEKETSETYSSSRKYEIYDNKDDGLDGLITVEGGKYTTSRKLAE
                     NCLAIVAKKMGRNLGRSVTDKQYLTGCDIKDLCLFIEEAKRPADGLSGATLEYLARNY
                     GTAYRDVVGLAREKKSLSETLNEDGEIMAQVVYAVRDEMAQTLADIVMRRTGIGTLGN
                     PGDDVLRKVAQVAADELHWDQQRMEKEVEAVVNLLKIPVD"
     gene            48507..>50151
                     /locus_tag="PLR13_00230"
     CDS             48507..>50151
                     /locus_tag="PLR13_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQC17782.1"
                     /translation="MISHYFRPNWTEKAPAAGTYRSIFKYGDLHSFKHPSDAWYRMIK
                     EEFHLNDKDFASRVKEGLEPVSLNRAQALKPQQMDCLRNIVGGENVALDDYSRVKYAS
                     GKTTEEMLELRLGIIREVADVVVHPRDKQDVQKIVQYCHQENIPITVYSAGSSVNFGC
                     RPVRGGVSLVVSTHMNKLLEVNEFNQTARVQPGMFGPAYEEGLNKAPELFKTKHRYTC
                     GHFPQSFEYSTVGGWVVTLGSGQASTYYGDAYDIVFSQEYVTPAGTFKTLDYPATATG
                     PKVNDIMKGSEGSFGILVEVTMKIFRYMPENRRRFSFMYPTWEAAVNASREIMQSEFG
                     KPAIYRISDPEETDRGLKLYGMPDFVDKILVMKGFKPMQRCLSLGNAEGDCDYTKLVV
                     RKIKTVAKKHGAISLTGYAAKKWEHTRYTEPYMREDLGDYDILIDTLEAAVTWDNLHR
                     LHQGVRAYIKNRPQTMCMTHASHFYPQGTNLYFIFIAHMKDPEEFRKFQRGIIDNIQK
                     HGGSLSHHHGVGRMIGPWMEKHLGKEQLDVLRTLKKHFDP"
     assembly_gap    50152..50251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            50398..51975
                     /locus_tag="PLR13_00235"
     CDS             50398..51975
                     /locus_tag="PLR13_00235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQC17783.1"
                     /translation="MSGEKNTTQDLVLSVDAGTQSIRAALIDVRGNIKAIVKTPIQPY
                     FSEHPGWAEQRPDYYWDVFCKTTHQLLQDQEVLKKHVKGVTLTTQRATMVNVDQNGNP
                     LRPAIIWLDQRKADSRKILPAPLRPLLKTVNLLDTLENVIQECEANWICQHQPDIWEK
                     THKYLVLSGFFTHRLTGEFVDSTGNNVGYLPFDNKTYQWAGKFDFKWRIFKVEKEKLP
                     DLVKPSEMMGTITKKASAETGIPEGLPVFAAGSDKGCEILGSGCLTPETGCLSFGTIA
                     TFDVATPKYVELHPMIPPFPASVPDTYYTEVSIFRGFWMVSWFKEEFGLQECMLAEKT
                     AEPPEKFFDSLIQDVPAGSKGLMLQPYWTPGREVDAFAKGSIIGFGDVHSRAYLYRAL
                     LEGLVYGLREGGELTQRKTKIPVTRLKVSGGGSQSDAAMQITADIFGIAAERPHTFET
                     SALGAAIDAAVGLKLYSDFASAVTNMTRTGRVFEPDKDNHGLYDNLYRRVYLKMYERL
                     KPLFRDIRDITGYPAGN"
     gene            52125..52550
                     /locus_tag="PLR13_00240"
     CDS             52125..52550
                     /locus_tag="PLR13_00240"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HQC17784.1"
                     /translation="MKKILFSFVMIVCLFWTTVVFAELKEGLWEITSQVEMKGMPQSM
                     PPTTFRQCITKGNPVPQNKDKNFECKIISQKVSGGTVSYQVECTGKEGVVQTSGTTTY
                     TDKAMNGASTTSFKMKGQPAMTMSNKIRGKYIGPCPKAQ"
     gene            52586..52768
                     /locus_tag="PLR13_00245"
     CDS             52586..52768
                     /locus_tag="PLR13_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17785.1"
                     /translation="MSFFIVLFEDGSLKIYKSMQDREFLPEGARQFKCSSNVTLQDLC
                     LWLDGGCKKLRTIKEI"
     gene            complement(52745..53413)
                     /locus_tag="PLR13_00250"
     CDS             complement(52745..53413)
                     /locus_tag="PLR13_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17786.1"
                     /translation="MFNVIRPEFVLAYFENRMLIFFPFFQLQRIVAEASPDKTDGQHK
                     NQKNQEQDQPGHHSAEDVRNAQPDHRPRLEYPGPNDIQKKQQQSKTVKKRYIRKKTCY
                     QTKQRGSHAGIFGLIGCDCVGNKFHFVFVIFSETYFDFNITSRSGKPLSSSWQRLFPH
                     RRLPQAVMIRNGHPKECCWSVLKIIHCGIPATGSPDDVRPGNGRFDFAKTTANACLDF
                     LDGS"
     gene            53437..53586
                     /locus_tag="PLR13_00255"
     CDS             53437..53586
                     /locus_tag="PLR13_00255"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HQC17787.1"
                     /translation="MDFFRDVWGFLKVRKKFWLLPIILMLLLCGALMIFAGGSAVGPF
                     IYTLF"
     gene            53596..55464
                     /locus_tag="PLR13_00260"
     CDS             53596..55464
                     /locus_tag="PLR13_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HQC17788.1"
                     /translation="MSPAILGISAYYHDSAAALLIDGRIVAAAHEERFTREKHTSVFP
                     VHAATYCLREGGRHLADLDGVAFYEKPYLKLERLLETYHAFAPRGLKSFMAAMPVWMK
                     EKIFMRKMLHDGIAGIGPGRVKLLFPEHHLSHAASAFYPSPFDKAAILTIDGVGEWAT
                     ATIGLGEGRSIKILRELDFPHSVGLLYSAFTGYCGFKVNSGEYKLMGLAPYGHADSGQ
                     TALYRRKILDHLVDIREDGSILLNMDYFDFATGLTMCRDEKWEKLFGIPRRQQESEIG
                     QPYMNMALAIQQITEDVVSRMARTARKLTGSNYLVMAGGVALNCVANGALLRQKIFDD
                     LWIQPSAGDAGGALGAAFAAWHVGMEQPRKSVSEQDAMAGAFLGPEFGSDDIKRTAKT
                     YQASFVHYEDFDELCAVVAERILRGLVVGWFQGRMEYGPRALGNRSILANPCLPDTQK
                     KLNLKIKYREEFRPFAPAVLEEDVAEYFDLDRPSPYMMLVAGLKEHRRRPQPENERQM
                     TLYERLYHMRSDLPAVTHIDYSARIQSVSKRTNERFWKLIKAYKQKSGWGLLVNTSFN
                     VRGEPMVCTPEDAYRCFMQTDMDCLVMGNDLFSKDDQPPWSEKNPWLAAGTPYAKD"
     gene            complement(55483..57642)
                     /locus_tag="PLR13_00265"
     CDS             complement(55483..57642)
                     /locus_tag="PLR13_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17789.1"
                     /translation="MTVYLLSIGLISILGQVILLRELNVSFYGVELIYLFALGIWLFW
                     TAVGVIAGRRMALPSRHSPAVLFILFGMILFLDVAFIRSSRLIFGGVPGAYLDFFQQL
                     LVATLSILPVGLLSGLLFQQTAGLYVDSGRTLAAAYAVESAGGLIGGLFSTLLIILDT
                     QNLFAALICSIVSFATPLLGAGKSLGAFLRRTAIVLLAMFAGLIFNASPLHDISTRWN
                     HPNLLDSSDSPYGRITVTRMFDQISVFENDALSFETEGTDAEYFCHLVMLQHPHPRKI
                     LILGGGMEGIIGEALKYRPDHIDYVEINPVMTKLVMEHLPESIRQSLQSPEVRIIYAD
                     PRRYLTNSGRYDLILVGMPEPSSGQANRFYTQEFFKQCAAKLKSGGLLGFKLQTAENL
                     WTGPLIGRNASIYHALRSVFPAVLFLPGTTNIVTASFAPLPDTPDIMTGRLQHLQLET
                     RLISDHYIRYLFTNDRFAAVKNLLHEKNDLLNSDIRPVCYQYALIIWLSKFFPRATLF
                     DFSSIMEKTLLRYLPVCFLMVLPVLFLVSRFLPGLRRKILVAAAGFAGIALETILLLY
                     FQLKYGVIYRDIGLLLMSFMAGLALGALAVDKLMARFADHRLGTRRCGIILVAGSCCL
                     CAITALLIRNSLPAGLWLTGLLLTATGFIVSGIFAYASLYEIDDQRAIISPLYAADLI
                     GGCLGSVLVSLIFIPLAGMDTTMWGMLLLSVYAFLLL"
     gene            complement(57647..59185)
                     /gene="amrB"
                     /locus_tag="PLR13_00270"
     CDS             complement(57647..59185)
                     /gene="amrB"
                     /locus_tag="PLR13_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQC17790.1"
                     /translation="MKWLRLLCFLPVIVFFLTSACQANKDAADGVREPAFAGKFYPAS
                     ADKLRLTIEKYLQDALPPRVKKPVAVIVPHAGYIFSGQICADGFRQAGHHPYDVVVIL
                     GTNHTSPGLQGVSLYPGEGFRTPLGIAAVDKTVIASLLTESPVDCMLDKSAHVSEHSV
                     EVIVPFVQIVFPNAKIVPAVVGTQDRHTSIRFGQALAKVLKNKNALIVASSDLSHYPS
                     RDNAVKVDRRTLDAVATLDPAVFQKTIQDTMSKNIPNLSTCACGEAPIIAALAAARLL
                     GADRGNVISYANSGDVSVGDSAQVVGYGAVVLTAGQKGISSSQTDLQKTAAVPFDLGT
                     ADKKALLAFARETLSRFFTTDTVPLARHFPAHLNHPRGVFVTLKKNGALRGCIGHLIT
                     DEPLVKLVGAMALQAALNDGRFPPLTAEELPDVDIEISVLTPMKQAARPSDIMVGRDG
                     VVLSKDGRSAVFLPQVALEQSWSRDDMLDHLCSKAGLEKRCWRQDATFSIFQAIVFSE
                     SQLK"
     gene            complement(59202..>60130)
                     /gene="amrS"
                     /locus_tag="PLR13_00275"
     CDS             complement(59202..>60130)
                     /gene="amrS"
                     /locus_tag="PLR13_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQC17791.1"
                     /translation="NGQCGRCRTRMNVKGELRSLVYGRPISVHIDPIEKKPFYHFLPG
                     SAAFSLATAGCPLKCKFCQNWEISQSSPEDFKVAFTPPAKLTSAARTSRSPVIAFTYN
                     EPTVFTEYLTDIARNARQQGIRCVMISCGFMNEAPLAEMCETLAAIKIDLKGYSEDFY
                     RSACQAELKPVLRSIRQIAKSRAHLEIVNLVVPTLNDSDKMLKGLIDWVMGEVGPDVP
                     LHFSRFHPDYQLRNLPSTPVATLERAYRMARDRGLHYPYVGNVPDHPGNNTYCPKCGK
                     IVVKRQGFFITGMNLRNGLCTFCKNRIAGVWT"
     assembly_gap    60131..60230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<60231..60553)
                     /locus_tag="PLR13_00280"
     CDS             complement(<60231..60553)
                     /locus_tag="PLR13_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HQC17792.1"
                     /translation="MISRRDFLKTLSGLAVTSGGTWMILSDPSWQRLFQEGHAGLAFG
                     DSYLDELIKSSPKARYWRPLEIKGSVTPKDQTLIQCLLCANSCVIANGQCGRCRTRMN
                     VKGELR"
     gene            60755..>61351
                     /locus_tag="PLR13_00285"
     CDS             60755..>61351
                     /locus_tag="PLR13_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17793.1"
                     /translation="MPNKINIPPDKQTGMIYALLAIAVLAVFSQLIRYEFVIIDDPVY
                     VIFNRHITSGMTADSFIWAVSTKYADLWHPLVWFSFMLDYRVFGLNAGGYHLTNLLLH
                     MMSSLMLFWLFHRMTGALWKSAFIAAFFALHPLHVESVAWISERKDVLSAFFWMLTLC
                     LYVVYTEKPVIQRYWPVVLGFVCALMSKPMVVTLPVVLL"
     assembly_gap    61352..61451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <61452..62393
                     /locus_tag="PLR13_00290"
     CDS             <61452..62393
                     /locus_tag="PLR13_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC17794.1"
                     /translation="PDSIPGLRLAGSVIFIAVVSVAVVRMRRRHPYLLAGWLWYAVTI
                     FPVIKIVQISSDAMADRYHYLPSIGIALMIAWGIPLFFKEENYRKKIMFPAAVLFLAM
                     LAFGSWRQCGFWQNSVTLLEHAVKVQKDVYVLHDYLGHALYFEGKSRAAIVEFNKAVF
                     LNPGYARAYVNRGNVYAAIRNYDEALKDYQTAVRLRPDEVDAHYMKGMVHSKTGRYQQ
                     AIEDFSQTIALDQNHARAFHDRGLAYFHLGRYEQAISDYDGAIRRNPDFTLAYHNRAY
                     GYFRQRNDVRGCPDAHRACALGDCDLLTAVQQEGRCR"
     gene            complement(62423..64120)
                     /locus_tag="PLR13_00295"
     CDS             complement(62423..64120)
                     /locus_tag="PLR13_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQC17795.1"
                     /translation="MFLPTTRDELKKLKWDKPDIILVTGDTYIDSPFIGVSVIGRVLV
                     DAGFRVGIIAQPDWHKPDDISRLGEPRLFWGITGGCMDSMVANYTATKKKRENDDLTA
                     GGKNNRRPDRAVVVYANLIRRYFKETKPLVLGGIEASLRRIAHYDFWSDSIRRSIMFD
                     ARADVLVYGMAEKAVVELAQALRDGKDTTDIRGICYLASAPPPQNALELPAYEDVLAD
                     KKTFIRMFNRFYCNGDPLTAKRLYQKHGDRYLVQNPPQPHLTTPELDHIYGLDFTRDV
                     HPFYSARGNVRAIETITFSITSHRGCYGECNFCSIGLHEGRTVISRSEKSILDEAQKL
                     AGRSDFKGYIADVGGSTANMYGIECAKKIKKGACNGKRCLSPRVCDALPADHSRQINL
                     LRNLRNVRGVKKVFVASGIRYDLLLNDRAHCLAYMKELVEHHTSGQLKVAPEHSEWPV
                     LKLMGKPQAQALLNFKHLFEKTSAVSKRKQFLTYYFIAAHPGCTEEDMRRLKAFTTRE
                     LKTNPRQVQIFTPLPSTYSALMYFTGIDPSTGKKIFIEKNMEKKEKQKNILIGNKRS"
     gene            complement(64156..64548)
                     /locus_tag="PLR13_00300"
     CDS             complement(64156..64548)
                     /locus_tag="PLR13_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17796.1"
                     /translation="MLFLLHIVLMVIATLGIMAGVSAAMFFRRKKNWLKIHKTLNTLS
                     LAGITVGIIMAFFYVAGTGGKHLNDVHQMTGLAAFTVVLIAIALGHYQFKALNKSAIR
                     AAHRWLGRVSIFILAAAVLLGLRLINLL"
     gene            complement(64579..65421)
                     /locus_tag="PLR13_00305"
     CDS             complement(64579..65421)
                     /locus_tag="PLR13_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC17797.1"
                     /translation="MIIRCWGSRGSIPVSGRLYQRYGGNTTCLEIRTKDNNILIVDAG
                     SGIREAGNALLAEGSLNFTLLLTHAHWDHIMGFPFFKPIYSSKIRMDIRGCPFAQTSI
                     KKMLSRIMAAPNFPVNYSHIRAKIVYHETCFKPVYRLGDMLITPIALSHPNGGTGYKF
                     EEDGKTFIFLTDNELGHRHPGGLPFRQYLAFCRNADLLFHDAEYKEAEYKKTRGWGHS
                     SYKDVLKLSLGARVKKLGLFHHNQERTDADVDAILKDCRDTVKKEGGKMNCVAVGQSM
                     QFTL"
     gene            65647..65847
                     /locus_tag="PLR13_00310"
     CDS             65647..65847
                     /locus_tag="PLR13_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQC17798.1"
                     /translation="MKSIKIKGMSCGHCVMAVTKALNAVDGVRNVEVDLKNGVATYEE
                     IKPVDSAVVAAAIKKAGYDVVE"
     gene            65831..68113
                     /locus_tag="PLR13_00315"
     CDS             65831..68113
                     /locus_tag="PLR13_00315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQC17799.1"
                     /translation="MTLLNKTPADGGLKKTVVAVGGMTCAACVRRVENAIKDVIGVRD
                     AVVNLANGHATVMHSTQWEGPAALARVVAEQGYEYLGEIKNHLADPIEASRLKELREL
                     KLKVACGAVLSVIIFFGSMQHWFPFLDFIPHRIMLLLLFVLTAPAVFWVGSRFFIGAY
                     KAALQKTSDMNTLVAVGAFSAYAYSAAATFSPQFFTTAGITPHVYYDGAAMIVTLILL
                     GRLLEAGAKSKTSMAIKNLIQLKPKTAHLIREGQELDVAVDEVRIGDVVFVRPGERIP
                     VDGTILSGESTIDESMLTGESIPVSKVAGQKVFAATMNTTGGFTFQATGVGAETALAQ
                     IIRLVQEAQGSKAPIQRLADKVVSVFVPAVFLIALMTFAVWYFLSAEGNFSRALISFV
                     SVLVIACPCALGLATPTAIMVGTGLGAQSGILIKGGEALEKIHKLSVIVFDKTGTLTK
                     GEPDVTDILTADGYEEKQILSFAAALEKSSEHPLARAIVRKAEQKSVRIETVEKFTAL
                     SGLGAKALMDGQRVLIGNRFLMTDEGIAIDDWNLSTERLTGEGKTVVYLALGKSVVGL
                     IALADTPKASAARTVQRLQSRGMKVAMITGDNDGTARAIARQLGIEKVMANVLPGKKA
                     EEIKNLQDAGEIVAMVGDGINDAPALAVADVGIAIGAGTDVAIESGDITLIRDDLLGV
                     PDSISLSQATMRTIRQNLFWAFIYNVVGIPVAAGVLYPFFGILLSPEFAAAAMALSSF
                     SVVSNSLRLRRVWEKKIARG"
     gene            complement(68139..68900)
                     /locus_tag="PLR13_00320"
     CDS             complement(68139..68900)
                     /locus_tag="PLR13_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQC17800.1"
                     /translation="MNEKLLKTYRLFLKDTIRKVTDFSQTDQSRGIPPPPIEKPYSGT
                     ARRLDLVRYDQFQNIGTLDLKTAIRNRESRRTYGTQPLTLGELSFLLWATQGIKQILD
                     AGHALRTVPSAGCRHALETYLCILNVQGIAEGIYRYLPLEHQLLHEQTVENLKLKIRQ
                     AVFGQRYPADAAATFIWTAVPCRMEWRYGIAAHKVIALDAGHVCQNLYLACEAIGAGT
                     CALAAYDQEEMDRLLSIDGEEEFTIYLASVGKKPF"
     gene            complement(68950..69429)
                     /locus_tag="PLR13_00325"
     CDS             complement(68950..69429)
                     /locus_tag="PLR13_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQC17801.1"
                     /translation="MMTLLWILAVLFILIGVAGTLIPALPGVPLILIGVLIGAWIDDF
                     QRISAATVVVMVILTVLGVVIDYVAAALSAGRAGASRQGLIGAVLGTLAGLFTGFWGL
                     LFMPLAGAAIGEFIARRDMLHAGKVGAATWFGLVISVVVKLAIAFTMVGVFIAALLI"
     gene            complement(69472..70596)
                     /locus_tag="PLR13_00330"
     CDS             complement(69472..70596)
                     /locus_tag="PLR13_00330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC17802.1"
                     /translation="MQTTTKDQSYSWISRFAIEHVASRNDLQTMQDFPLDIWQKMGEA
                     GFFKLGISPQFGGSGGGLTQLAGAGEVFVRSGFNMGLALSWLYQQIIAIHLMSGFGTE
                     EQKKRYLPLMANGSLTVSFAVSEPKHGARPKLLTSTADLQDTSYILNGEKTYLTNAPI
                     AGLFIVIAITGRDKDQKRFSAFLVPADHEGLMIKPRLKFDFLKPAPHGGIKLNNCRIP
                     ASAILGEKDSAYANIVIPFSDAENTVLMGAVTGGMGAELMDLIMESDKRNIHKDKALQ
                     SEVGALDSLLETMRAIVRETAKKVDERDNAAVPLIVTFKELAAGFQARIAAAIAKWDI
                     KTPDRFKVLQTDINVLGALQEKTLQDKQAKLGDELLHGAR"
     gene            complement(70598..71977)
                     /locus_tag="PLR13_00335"
     CDS             complement(70598..71977)
                     /locus_tag="PLR13_00335"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQC17803.1"
                     /translation="MVGLIQQFVSCCRASGMRISTSEVLDSLHQMKLINPVDEQQFRS
                     MLRSNFAKSMREVQHFDRLYHLFFHEMRIDTSDIRQAADYSTEINMTGDALKKIPHDN
                     PIYDAVVDFLQGNPMALLREMRRIRTEEDVTMLRFNLGPLGNRLNVMLQINKAASDAR
                     TLVEDHYRKTDASAGRKLAAYFEERVSAARSMLVYEPQQGESGTRKTKTEEQKLKGLG
                     EKSFSSFTRQEVEEMREAIDKMVRKLKNIVSRRYAVRNRGSIDIKKTLRASAKYNGVP
                     VDVKYRSRAKRKSRIVTLCDVSGSVWAAARFMLNLLYALQDCFDKVNSFVFIDHLSDV
                     TSIFEKNEINEAIRLVLEKPDINFNASTDYGETLRNFKKDYMDLLTKKTTLIIVGDGR
                     TNYMNPEDAILGQMRERCRRIIWLNPEQENLWSSGDSEIGSYMPHCHEVRQCRNVNQL
                     FSFIEELVL"
     gene            complement(72169..73077)
                     /locus_tag="PLR13_00340"
     CDS             complement(72169..73077)
                     /locus_tag="PLR13_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQC17804.1"
                     /translation="MEYKNVDDVQSSLTDAGYIPSREIATVVFLAAATQKPVLVEGPA
                     GVGKTELAKAIARSLERDLIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKDRLNDIIS
                     SAATLTEAVDQIAAQEDAFFSDRFIQHRPLLQAISSEKPVVLLVDEVDKADPEFEAFL
                     LEILSDFQVSIPELGTRKAIQIPFVLLTSNNYRDMSDALKRRCVHLYIDYPARDTEIS
                     IVRLKIPGIEEKLCDALVDAIRKIRKLDLKKNPSISETIDWAQSLIALQINELTPEVV
                     SETLNIICKYQADMEKVRKNINRVLQ"
     gene            73360..74742
                     /locus_tag="PLR13_00345"
     CDS             73360..74742
                     /locus_tag="PLR13_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQC17805.1"
                     /translation="MTDSPIKSALKDFFFFWKPSLARRLTLSFTIFGLVIGYAVLIYL
                     AVSSTNTLIRQASVSVEEYLTYMSREDLRENQGELLKFIDQKMTNIVHMAKAMQVVLL
                     PVQFELYFKKDGQWQHTFTDEQGIVTSEIISNPGLAGLLEVAKSRKMTTSSKFFYGRH
                     DKVNVKINISPDKNNYTQLLAFDIYRVGILKILRENIYKAFFFFVLLLVICHMMGHIF
                     SYLLARPIEQLSREAEAIASGQYERRFTSNRQDEIGKLADALNIMASRILDSTKEREN
                     IFIGILIALTRAIDAKSPWTAGHSERVTKFAEAIGHNLHFNEDEMRALTISAILHDIG
                     KIAVPEQILDKPGKLTDEEFAVVKKHPQAGADIISSIPSYESILEGIVHHHERWDGKG
                     YPKGLHEKDIPLIARIICIADVYDALTEDRPYRRAWSRTEVRQFFEEQKGKMFDAELV
                     DVFLTLPDVQ"
     gene            complement(74875..75024)
                     /locus_tag="PLR13_00350"
     CDS             complement(74875..75024)
                     /locus_tag="PLR13_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQC17806.1"
                     /translation="MCSVCGYVYDPVKGDPDNGIAAGTKFEDLPDSWTCPICGADKSK
                     FEKEK"
     gene            complement(75127..75567)
                     /locus_tag="PLR13_00355"
                     /pseudo
     CDS             complement(75127..75567)
                     /locus_tag="PLR13_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            75765..76355
                     /gene="fabA"
                     /locus_tag="PLR13_00360"
     CDS             75765..76355
                     /gene="fabA"
                     /locus_tag="PLR13_00360"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HQC17807.1"
                     /translation="MKYQEFLKRARFDFEEILAFAYGKLVDDPPEHFDAKFPAPPFLM
                     VDRILSIESDGKKGKIIAEQDIRPDAWYFQCHFQGDPVQPGCLGVDGLWQLLGFFCVW
                     RGALGTGRALGCGDVAFNGQIRPFNKCVRYEVDVRRYSMLKESGASIVIGDGRVYVDH
                     ELIYTVGQARVGVFKDIAYKDYPRRSKYSIGGIMER"
     gene            76440..76856
                     /locus_tag="PLR13_00365"
     CDS             76440..76856
                     /locus_tag="PLR13_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17808.1"
                     /translation="MNIVKKTAAYFARKEFCRNAINECADLSLLKEKHTLGVTIGIVL
                     IVLSYIIVLPLFFMVGLMAARLRKPMVGVIGIPLAYGFSWLIMMLGMYLTGPEYAKTL
                     GKCVVRVVLEKILGDEAKIIASKPNKNIDMTGDANE"
     gene            76849..77121
                     /locus_tag="PLR13_00370"
     CDS             76849..77121
                     /locus_tag="PLR13_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQC17809.1"
                     /translation="MSNTEEMIRELKIKIIETLGLIDVGPDDIQDDERLVGGDLGIDS
                     IDVLELVMMIEKDYGVKIDSKKLGVKVFASVSALADHILNHQKKTV"
     gene            77153..78505
                     /locus_tag="PLR13_00375"
     CDS             77153..78505
                     /locus_tag="PLR13_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HQC17810.1"
                     /translation="MMNVLLISANTLKNPYPVYPLGLDYVAGCLDYRYRTKIVDLNVF
                     PHPETLEAMIREFSPDVVGISIRNIDNTDAINCRSFLTEYETLVGQIRKNSRAKIILG
                     GSGFTIFPREFMQALDADYGIVGEGERLALLLDAWEKNEDVNKIPGVLTSVKTDIVYE
                     PFGGRLNRHFDPEETHTRFYLAYGGMLNLQTKRGCPFRCGYCTYPHIEGGKMRCYDPE
                     EIARSARALQDAGAKYIFITDSAFNASYEHSAQVAKAFTKAGVSIPWGGFFASTVPPA
                     DYFKRLADAGLTHVEFGTESLSDQVLENLRKPFTSENVFTAHREALDAGLYVAHYFLL
                     GGPGENKETLQATLMGIDKLERAVFFFFCGIRIYPHTALYDVAVREGQIKAGENILPP
                     VFYRSQGIRVEEIMKTVENHANGRLNWLIGAGEAKATRILPKLYRRGFTGPLWEYLIQ
                     "
     gene            78747..79511
                     /locus_tag="PLR13_00380"
     CDS             78747..79511
                     /locus_tag="PLR13_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQC17811.1"
                     /translation="MTGVILLLAAAVIFFFHPLAAAAVALFYIVLCVAASFFPQSSFY
                     LPVISRGRTGRNVVALTFDDGPAEPTTRRILYLLDRYSVKATFFVSGVNALKHPDIVR
                     EIIRRGHTVGNHSFHHNPFLMLGSYRYLYNEVSKAQDILKDRGVNTLIFRPPVGIISP
                     KLPSVLDKLGMFCVTFSCRGGDFGNRRIRNLSSRILHSVKADDILLLHDALPRGGGNQ
                     AVLLVEIEKILRGLKKQGLNVVPLADLIGRHVSIMM"
     gene            79639..80010
                     /locus_tag="PLR13_00385"
     CDS             79639..80010
                     /locus_tag="PLR13_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17812.1"
                     /translation="MKNQWNLLKETREADSNDIEALADVPADSPWFSGHFPGEPILPG
                     IALVYAVKQAIHREAQKKGERLELHTLKRIRFMQPVRPGDQLSLNIRAAEADGERLFH
                     FKIFCRDTMVSSGMIAAKKMM"
     gene            80050..80322
                     /locus_tag="PLR13_00390"
     CDS             80050..80322
                     /locus_tag="PLR13_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQC17813.1"
                     /translation="MPASQDVKGIIRRVAEIIIDELKLEEATADTFDPDMDLVDELGI
                     DSMDLATIALVLQDEYRITIDEDDYPQLKTIRLISEYIKERLPVRH"
     gene            80323..>81407
                     /locus_tag="PLR13_00395"
     CDS             80323..>81407
                     /locus_tag="PLR13_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17814.1"
                     /translation="MTECEMANQVNNKVNTESVKPKFSELLAHPEVKARWEKVRKYFF
                     LRESTYDMTNRCNIRCDGCYYYEGEKQFAKEVGDVEAWRALMRAEKARGITYVVLAGA
                     EPSLVPELLEVCYQEMPLGSIATNGFRKIPPSVGYKLHISVWGSDETSLKIRKAKNLL
                     KKQIENYENDPRAVFVYTFTRNNIDEVNEVTEELAAHDCKLTFNVFSAPVGYAGNLRH
                     DDASLEQTRQTMIELLNQYPRNVLFSVYNAIAHTHRKGLHDLYSCSYPRQNPSQDIGL
                     GRSFRQYRTDLNWDRSVACCVPDTDCADCRHYAAGSAVVTARLYRHVTNLATFKSWLD
                     YVDTYLAVWVMNYEKGENLCNEIIEPPR"
     assembly_gap    81408..81507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            81658..82656
                     /locus_tag="PLR13_00400"
     CDS             81658..82656
                     /locus_tag="PLR13_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17815.1"
                     /translation="MKTVSSLLDKEWHDRYRRISNLNIRSSIYDVTNRCNLRCKGCFF
                     FSSGEHQAAVEEMDIGKWEAFIDREKERGVNLAILIGGEPTLCLDRVEAFYKRLPTYC
                     ATNGLIKVPRDRFPDMMVGISLWGSAEDEKILRGRDTFAVSSKNYEGDAYTYYLYTIT
                     PRQVGKIEPVIKRIADVGLKVHLQLLSNDEGVDGFSWQEGELKDLCHEMDEMLDTYPQ
                     TVISCKYYHEIITTGKMMGRRFGWNECPSVTEALDNRANRPRRLIHFARWASDLKTVH
                     RCCTSATRDCSTCKDGAAHMSWVMVNKREHLKSTRDLQNWIEVYEMFAKLYHFIPW"
     gene            82746..83996
                     /locus_tag="PLR13_00405"
     CDS             82746..83996
                     /locus_tag="PLR13_00405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQC17816.1"
                     /translation="MGLKKTVIVGYDAVSSLGAQLDAQWRQAVAGKSGIGRLTRFPLS
                     ENFPVRVAGEVEEIDPGPYPFLQPREMAHWTSPIFKYALLVVHRALQNSGIEITRDIA
                     PRVAVTFSSAVGGLDAVLQADRLMVKDGKLPHPFTNPNSCINMVGGKISILTGATGPI
                     CSTITACATGITSMIIGEMLLQRNMADVVIAGAVDFPLVEPIVAGFATMNGAYRPKEG
                     QPEEPPEKTSRPFSANRRGFVVSEGAGCIILATGEFAKVHGLPSGMEMAGWAMTSDAS
                     HFVSPNLATVRKCIEETIANAGLKPEDIDAVNAHATSTKIGDKVEADALKNIFGGHVP
                     PVSANKSQMGHAMGASSAIEAILAMEGMKKEIVLPTINYRPDQDIAIDCVAEGARNLK
                     QEFVLKNAFGFGGCNSCIILHRTE"
     gene            84088..85353
                     /locus_tag="PLR13_00410"
     CDS             84088..85353
                     /locus_tag="PLR13_00410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQC17817.1"
                     /translation="MRAPKNRRVFVVGYGAATPLGSTFEKTWKRAVKGEAGFRKVTRC
                     KVESACDVVGEIPDWYPMELDFTDAKEVYNWNAAFVILTMAVCKEALENAGIVVDAQI
                     APRMACLIGSALNGSDAFRIAVHDLDHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPQGACASGNHAIGIGARMIRDGDCDFVLAGGADTPILPELIHGFANMNATIKVN
                     PKDRAYDNPALASRPFSADRKGFVLSEGAGVVVLAADEAIKTYGLKPKAEVLGIGWTS
                     DAHHYTSPNMPTIIRAIRETIDDAELEPTDIQYINAHGTSTPKGDTTEIKCLREVFGK
                     KIEKIPVSSNKSQLGHTLGATAAIEAALTIEGMKKGIILPTINHVPDPEFSDIDVVPN
                     EARKNKCDIALSNAFGFGGTNCCVIFRGV"
     gene            85355..85834
                     /locus_tag="PLR13_00415"
     CDS             85355..85834
                     /locus_tag="PLR13_00415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQC17818.1"
                     /translation="MKPKPFKPEIYHNDERYVRDLTTGLVWHRSVYRVLYIDTDRSQV
                     VYHSNYLRYFEFGRTSLMRDLAYPYKEIEDGGYVYPIFDLGISFHQPLYYDDVMYIHT
                     RPVNLERVRLQFDYLITHQEKGNIICAGFTKHCALNLSGTPVAVDAKTVHLWKTFPV"
     gene            85813..86676
                     /locus_tag="PLR13_00420"
     CDS             85813..86676
                     /locus_tag="PLR13_00420"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HQC17819.1"
                     /translation="MENISGLNERQRYPFDLEIYPYLRDHHLENRAILPAVESLIILA
                     GACRAVSLQSEIKCIQKAAFSRFLYLAPDNHRQPAVIDIIKTEGGNMIASLLTSVQSK
                     TGTISRHVEHARAECVISGFEETGPPSFRSVDKLKGSCISIPSATVYRDLVPFGIAYQ
                     NIIGDLSVSSEGALGYVSGGNFEADDELLGSPFPLDAVMHAACVWGQRFAGKVCFPVG
                     FEKRLIYQKTEKGQEYLGRVVPVSITRESLIFDVWIYKDDVLYESVSGIIMKDVTNGR
                     MNPPDWIKVNS"
     gene            86779..87372
                     /locus_tag="PLR13_00425"
     CDS             86779..87372
                     /locus_tag="PLR13_00425"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQC17820.1"
                     /translation="MGKTFSIFLLAYLAGSVNFAIIFFKLTGRKDPRLGFSGNAGTTN
                     VYRQAGMVWAAVIFLLDIGRAMLVAVLAMYYLEPAWLPWAGYFLVLGNSYPCFHEFRG
                     GKGVANYLGFTILVSPWAAMLSCAVWLLVYGLVRMPFIASFFMVFTLACGQAYHSHWN
                     WGAIAGSLMTWILILYNHRINIMAHRTREKDVPHQNK"
     gene            87683..89059
                     /locus_tag="PLR13_00430"
     CDS             87683..89059
                     /locus_tag="PLR13_00430"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQC17821.1"
                     /translation="MKINEAECLEKWSSMAASSSLVDSSLYAKYDVKRGLRDIKGKGV
                     LAGLTQIGEVQSHTQIGDDESELGPGCLIYRGIDITEIVSGFVADKRSGFEESCYLLL
                     FGELPDKEELERFQKLLISYRSLPDDFIHDSMLKLSSRDIMNAISQSILSMYVLDDRA
                     EDIEIPNVLRQCIKLIAVFPLLLVYCYQAYAYRYGNQSLVIHNPGRQLSTAANFLHML
                     RHDSKFTALEQKLLDLALVLHAEHGGGNNSSFTVHVVTSSGTDTYSAIAAAMGSLKGP
                     RHGGANIKVVRMFEDLKNNISNWNDDRQIEDYLIKLLNKQAFDRAGLIYGIGHAVYSV
                     SDPRTLILREYVGKLAQEKGLSEEMKLYERVESIAPEIIGHERKMYKGVSANVDFYSG
                     FLYRLLDIPPELYTPLFAMARIAGWSAHRIEELSNGGKIIRPAYKSVTPRRHYVPMKD
                     RNSNEQNR"
     gene            complement(89179..90102)
                     /locus_tag="PLR13_00435"
     CDS             complement(89179..90102)
                     /locus_tag="PLR13_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQC17822.1"
                     /translation="MNIVISGSMAYDRIMDFPGYFSDHILPDKIHMLNVCFQVNGMKE
                     KFGGTAGNIAYALRMLGEHPIICATIGHDYQKYFKWLQQNGISTESIKVIDEEFTAGA
                     YITTDRSDNQITGFNPGAMKYQCKIDFDKFDSRETIVIVSPGNFNDMVEYPRACKAKG
                     IDYILDPGQSLPMWDKNDLIQAIEGCRILIVNDYELSLIMSKTGLNKNALLKMAGTVI
                     TTLGELGSQVSTLTSDIAIPPVKLKKVVDPTGAGDAYRGGLLSGLVQGKDIVTSAKMG
                     SVSASYAVETYGTQEYSYTSEEYHQRLNDLL"
     gene            complement(90184..91521)
                     /locus_tag="PLR13_00440"
     CDS             complement(90184..91521)
                     /locus_tag="PLR13_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQC17823.1"
                     /translation="MPENNFDIIISGGTLLTMSAGMDIIEDCFVGIHNGIITDIRSLK
                     KTRSTSIRAHETINADGCIVMPGLVNTHTHLPMVCFRGLADDLPLMEWLTGHIWPAEQ
                     RFVSKKMVYDGATLAMAEMILSGTTTFCDGYFFEDSLAEAVHVAGMRAVVAQGFADFV
                     MPDSAAVKKMMAAANRFVKKWYPHAPLLTPAFFCHSPYTCSPATLSAVKQSAREHGIL
                     YLVHLLENKDEVHTIRQRYGRTPVQHLLNLGVLDEQTVAVHCNWLTSEDMKIFADLGV
                     KVSHNPESSMKLASGVAPVPDMLKEGISVGIGTDGCASNNDLDMFREMDTTAKIHKVT
                     SLDPTVMNAESVCRMATIGGAHVLGLDKRIGSIETGKQADIILVDMNQPHLTPVYHYY
                     SQLVYAARGADVKTSIVNGKVIMKDRRLLTIDVPTAMKNVRTVAGGIIRKDSH"
     gene            complement(91508..91900)
                     /locus_tag="PLR13_00445"
     CDS             complement(91508..91900)
                     /locus_tag="PLR13_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17824.1"
                     /translation="MKRKIVISAFALSFLFLVSMICFSTAEVCYADAPKDVKIEYDSG
                     AQTLTVTITHKSAFPGFHHIKNVDVKKNAVSVSQNAYSSQPKEVPFSYVYKVAAAPGD
                     KLDVTVVCNMSGSKTATLTVPAAGNAGK"
     gene            92069..93376
                     /locus_tag="PLR13_00450"
     CDS             92069..93376
                     /locus_tag="PLR13_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQC17825.1"
                     /translation="MSAVILARLQFALTICFHFLFPAMTLGTTLIILISETLYLVKKD
                     ETYKKITDFLVRLLALIFVVGAATGIVMEFSFGTNWSGYSRAVGDIFGPILAAEGVLA
                     FFLESVFLGVLLFARNRVSPKVYWLSALLVFIGGHLSAFWIVVANSWMQTPAGYTINE
                     AGKIILGNFGDAVFNPSTVVRYVHTVIASWITSSVMVAGIAGYYVRKGLHGQTARTML
                     KIGIILFALTPLLQLGAGHAHAVNVIDLQPEKAAAMEGHFETAQGAKIYALGYVDEKN
                     RKTYGIYIPKGLSFLYNFDWNSEIKGLNDFPKEDWPPVNFVFQVYHIMVGAGVVMIGL
                     GLLGAYLLWKGRLYDNKWYLFMLPLLIPLPHIAHETGWIAAEVGRQPWIIYRLMKTSD
                     AASVVVSAGEITFSLIMFSLIYLLLGVMFIKLAITIVRKGPAA"
     gene            93421..94431
                     /gene="cydB"
                     /locus_tag="PLR13_00455"
     CDS             93421..94431
                     /gene="cydB"
                     /locus_tag="PLR13_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQC17826.1"
                     /translation="MDNIQCYQELWFVLILVLLMGYSLLDGFDLGIGALLPFLGKTKE
                     EKDILINSIGPVWDGNEVWLITGGGALFAAFPHAYATAFSGFYLAMMLVLFALIFRAV
                     SMEFRAHDEKRSLLWEKAFIAGSALAALLFGVALGNVVYGVPLDDQMEFTGNFFTLLR
                     PVPLMFGVTGFAAVLLQGAAYAVMKTEGELRERSFKAVSFLTVINLITAMIYFGTIAF
                     TFDDIVTNWLFYLAFMCTVLWLSSIFFSVRHKNDSAPFWESSFAFIGLWLVVAAVHFP
                     NLIRASNDQSMSLTIYNSSTSLLTLKVMSVIALIGMPIVIAYTIFVYRIFKGKTKSSS
                     HY"
     regulatory      94482..94583
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            94758..95561
                     /gene="thiM"
                     /locus_tag="PLR13_00460"
     CDS             94758..95561
                     /gene="thiM"
                     /locus_tag="PLR13_00460"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HQC17827.1"
                     /translation="MRMTAKEIYRSIELIRSRAPVIHNITNYVVMNNTANALLAIGAS
                     PVMAHAQEEVEDMVDIASALVINIGTLSESWIRSMFKAARQARKKGIPVILDPVGAGA
                     TNYRTNTARALMAQEPPSIIRGNASEIMALYNEQSKTKGVDSASSSDQAIDTAQKLSE
                     MHECVVCVSGATDYIICPQTILKIKNGNPMMSRVTGLGCTASALCGAFAAVGESTFAS
                     TAQAMALMGVAGETAAEVAAGPGSLQVHFLDALYRISEDDIARRLKVED"
     gene            95564..96187
                     /gene="thiE"
                     /locus_tag="PLR13_00465"
     CDS             95564..96187
                     /gene="thiE"
                     /locus_tag="PLR13_00465"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HQC17828.1"
                     /translation="MRGLYLVTDRRLCKDKSVEEIVLQAVKGGVSYVQLREKEMSTHD
                     FVEEALRIKKSLTPHKVPLIINDRVDVALACGADGVHIGQEDMPYDLARRLLGPKAII
                     GLSVETWADFIASQALDVSYIGVSPVFATPTKTDTKGVWGLEGLSRIKAFSRHPLVAI
                     GGIHEANIREVIEAGAPCIAVVSAICSSPDPETAAGRLARLINAALK"
     gene            complement(96263..97864)
                     /locus_tag="PLR13_00470"
     CDS             complement(96263..97864)
                     /locus_tag="PLR13_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQC17829.1"
                     /translation="MNAEAHLKAMDSMIHLCAAVKNVQLYSEDSPITQNSIDRAYLYL
                     LDTLKQKSPLVFAESGKKVMLCGEALREKDQETLHVVFLMDLFVNFGLRSISFEKGLL
                     KNELQAFIKSLARDPEHLRHLGGLAKTITDNGIIHIHADKQDVEFSLAGDQTEPQPVP
                     DPEPVPEMTNLPEINQPPVVIATDDPVSEIIAKTIESLRRLNELKNAPEPFVAEEQTN
                     LIRKSAGHVAEWLKIESVFTPDYVKNLDSLQKLVQALIHHELLTEANSIISVLSQINT
                     GVLEKNDDIRDISLQVLKNLASENNIHFLFKEININEKNKTTDAFKILSGFGDDFIIT
                     KLLDIVRHAKDSKERIRIIHIIQEIGQKAIPAIKENITTEAPWYFLRNMAYILGRIGD
                     EENVQILRPLLFHKDKRVRAETLKSIGQIGGNRRGALLLSALPDADFELKLHMIEMLG
                     KIKCTEAVDPLLDMLKGKSSMTKEQQVSFQETICAALGFIGSPEAIPILSEIAESKSF
                     LGIGSYPAEVKYAAAGALKSIKRKQ"
     gene            complement(97892..98341)
                     /locus_tag="PLR13_00475"
     CDS             complement(97892..98341)
                     /locus_tag="PLR13_00475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQC17830.1"
                     /translation="MTQVYQYKLTVPESAVDINGHVNNVEYLQWMQWAAVLHSDIRGC
                     TKATLDVGATWVVRMHHIEYLRPAFAGDTVVILTWVSDFRRVRSLRKYKVIRCKDKAV
                     LVEGKTDWVFVDAKTGRLRSIPKNVMMKFKLLPEVLEPEILNSLNFS"
     gene            98502..100334
                     /locus_tag="PLR13_00480"
     CDS             98502..100334
                     /locus_tag="PLR13_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC17831.1"
                     /translation="MVKEKESNVAGNLESYYTKPNNLVDMFEDSAAKFASRNLFGTKN
                     KETNQYEWVTFAQVAERVNNLRGALKKIGFEKGEKVGVIVGNSAEWFVCANATHGLGG
                     VFVPMYEKELQKVWQYIIKDSAIKYLFVSNQKIYEIIKNFQNDIPTLKGVFMLYGSGE
                     NSLAELEKMGKANPVLSYKPHWSETAVIIYTSGTTGDPKGVLLSHGNLTHNAKESHWT
                     FDVGEEEVGLSILPWAHSFGLTTDLHVFVYGGWQLGLAESADKLLLNFGEIKPTHMNA
                     VPRVFNIIYDKIQQSVSADPEKKKIFDAAVAEAIKNRDLKEKTDEFKFYDAVAFSTVR
                     NIFGGRLKQVVIGGALMKPEIAMFFSDVGVVPYVGYGLSETSPVLTNNSPKRGNKFGT
                     IGKPYKDTKVVIDKSRVGEDSPDGEIVVYGAQVMQGYHNKPETTKEVMMPDTWNGFPG
                     VRTGDRGWIDEDGYLNITGRFKDEYKLENGKYVHPESIENEIKLLRYVSNVIVYGEGR
                     TFNVALVVPDFEAVKTDSQTSRWAQGTPDETIANKECTDFISKQITDHLRKSFGGYEI
                     PQKFLYITEDFSVDNGMLTQTLKLKRRNVMEKYGERLRALYDNV"
     gene            100404..101615
                     /locus_tag="PLR13_00485"
     CDS             100404..101615
                     /locus_tag="PLR13_00485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC17832.1"
                     /translation="MNNQNDVVIVSAVRTPFSKFGGALKEIHSTDLGVIVINESLKRA
                     GMTGEDLDEVYYGMCDQAEAALYDNVNARQAILRAGLPSTLVSLTLDRACCSSLCAVQ
                     LGRRAILLNEADACMAVGAENMSNTPLLINGHRWGSGMKPLLVQDYLNPITYREYTPL
                     GRDAGEIAIEHGISREEQDAWALGSHQKWDAADKAGLFYDELVSVELPAKKGTPVIFG
                     KDELPRPDTTLEALARLKTIYGSPTVTAGNAPGLDAGATAVIIMRRSKAEKLGIRPLA
                     TIVAVASGAGEPRRMAELPAKVIAQALKRASLCIDDMSVIEINEAFAAVTLVSTKMLA
                     GGDEAKWRELLKKTNPNGGAIAIGHPVGASGGRILMTMMYELKRRGGGYGICGICGGL
                     AQGDAVVIRVD"
     gene            101708..101953
                     /locus_tag="PLR13_00490"
     CDS             101708..101953
                     /locus_tag="PLR13_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17833.1"
                     /translation="MRDEKGIASWVVIVIIVLLIAAGFLWSKYFAAPTATVMMQGADQ
                     AKPAIEKAHQASDAADRANKIAEEAAKRLNEDAAARQ"
     gene            102181..103161
                     /locus_tag="PLR13_00495"
     CDS             102181..103161
                     /locus_tag="PLR13_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC17834.1"
                     /translation="MKKALSFVWILVLLAFATVAQAEVKAGSFSVTPFIGGYVFEGNE
                     YDNNEDTYAAGLRAGYNFTKNIGVEAFFNYVPTNNKDWNDDNEMELFGYGLEGLYHFM
                     PESRFVPFFAVGVGGIHYDALPEDPDNRDKFALDYGAGFKYFVTDNIALRADVRHIIP
                     FNERYNNLLYSVGLTFAFGGEKKIVETKVEEPLPPPPPPAPVAKPVIIEKGRQTLDVK
                     FDFDKATIQDGYYKDIDALVEVMKDYPELNVVIEGHTDNVGKAEYNKKLSQKRAETVK
                     KYMVEKGGVDAARIEAKGFGDERPVDTNDTADGRAKNRRVEAAVDYIIEK"
     gene            103596..104606
                     /gene="aroF"
                     /locus_tag="PLR13_00500"
     CDS             103596..104606
                     /gene="aroF"
                     /locus_tag="PLR13_00500"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQC17835.1"
                     /translation="MIIVMKSHATEKQIEDVIHWIESVGYKAHPSIGVERAIIGAVGD
                     NRGKDILKFAESLPGVEKTMPILKPYKLASRESHDGNTEVTVGEVTVGGPEFVVMAGP
                     CAIESEEQLLESAYVVKKGGAQILRGGAYKPRTSPYSFQGMEEEGLKVLNKVGTQAGL
                     PTVTEVVNPADVDLVESYADMLQIGARNVQNFALLKKVGHSRKPVLLKRGMMTTIEEL
                     LMSAEYILSEGNPNVILCERGIRTFETATRNTLDISAIPVLKGLTHLPVIIDPSHAAG
                     NWKYVIPLSRAAVAVGADGIIVEVHPEPEKAVSDGAQSLKPEKFYQMMDEIRAITAIM
                     KH"
     gene            104637..105731
                     /gene="aroB"
                     /locus_tag="PLR13_00505"
     CDS             104637..105731
                     /gene="aroB"
                     /locus_tag="PLR13_00505"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQC17836.1"
                     /translation="MRSIKVNLDRKSSSSYEIRIGKDISDRLSLLIAKNHKAARYAVI
                     TDNIVGELYGKKFLTALKEIGLNASLIDFPAGESSKTIKTVVDIAETLAVSGADRETC
                     LIALGGGVVGDIAGFVASIYMRCVPYIQIPTTLVAQVDSSIGGKTGVDLPCGKNLAGT
                     FYQPQAVMTSLHFLNTLPPKEFNNGLAEIIKYGVIDDEKMFASLEDIMTAVKSKDPKI
                     LLGVVEKCCQIKKSIVEIDEKEGGVRRILNFGHTIGHSLEAVSGYAITHGEGVAIGMV
                     AAARLSARMGYLKNNEAGRIETLIDQAGLPTRIPKTLSGGKIVAQLKMDKKRKGAAIH
                     FVLLKKIGLPFIHGNIEQKMIADVIEDMKS"
     gene            105728..106810
                     /gene="pheA"
                     /locus_tag="PLR13_00510"
     CDS             105728..106810
                     /gene="pheA"
                     /locus_tag="PLR13_00510"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQC17837.1"
                     /translation="MKPHLLENLRKKIKDKDKEIVRLLNERAQISVQIGKMKGQGGMQ
                     VYDPAQEAKVHGYLRDLNAGPLASGAVSAIFREIISASRDLQKPMTIAFLGPEASFSH
                     LASRLHFGESSLFFPQTGIFRVFDEVEKGAIDWGVVPVENSLEGSVNVTLDRLMITPM
                     KIRAEIYLRIRQCLMSSAKNMKEVKRIYSHPQALAQCQVWVRTNLPNCVVCETESTAA
                     AVQMIRGKKHEAAIGSSLAAKIYGVNLLAEGIEDNPSNMTRFLVIGQGANDPTGNDKT
                     SLIFATPHSPGALHAALSAFARRKINLAKIESHPVKDKLWEYSFFVDMIGHADDRRVQ
                     GCLRELQNKTSFMKILGSYPKSEDAL"
     gene            106807..108381
                     /locus_tag="PLR13_00515"
     CDS             106807..108381
                     /locus_tag="PLR13_00515"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQC17838.1"
                     /translation="MICIPITSDNPKEALHAIERSCQLADFLELRMDLIGNIGLAELI
                     SAVRAESAAVKIIVTSRKKEEAAFVGRWPRRRNACSKTRYKIALLKEAVERGADYIDI
                     ELAEGTQAIRQLQALCAEKGSQTKMIISYHHLKDTPDLATLKKIFHRCAAYKPAVVKI
                     VTTARSEEDNLIILNLIAYARRKSQKIISLCMGEKGGISRAVAPLMGNFLSFAALDRT
                     GRSAPGQFTVHEMNRIQKWFKGKNRAPQSSASSPHASLPRHYVLLGNPVEQSLSPVMH
                     DAALKKTGLAEQYVAFCVQDIGGAMDGLRAMNIHGASVTIPFKVAVMEYLDDIDADAL
                     EIGAVNTIVNENGRLIGHNTDWAGLILTLKKAMTIRHKTIVIVGAGGTARAAAYGILK
                     EGGCPVIVNRTPKKGKMLAEKMQCAFYPLSEIGRIRGDVLINTTPVGMYPRGNQSPVS
                     AAVLSDYDCVMDVIYNPIKTKLLRDAEKQGCRIVTGVEMFVNQGAEQFRLWTGKEPPH
                     AVMKKIILERLSKGGR"
     gene            108371..109627
                     /gene="aroA"
                     /locus_tag="PLR13_00520"
     CDS             108371..109627
                     /gene="aroA"
                     /locus_tag="PLR13_00520"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQC17839.1"
                     /translation="MDDNPIRCPDPAVTVRVPGSKSLSQRALVAATLAQGDSMISNLL
                     VSEDTLYMIGALRELGAKIVDAPDGFQVTGTAGKIENSGRDLFLGNNGTALRFLTALV
                     CLGKGQYVLTGEKRLCERPVGALGDALRDMGVDIRTHHHCPPVTINAGGLAGGKITLR
                     DIESSQYVSALLLCAPYTDRGIELSLEGGVVSAPYIDLTIAVMEDFGVTISKPERFAY
                     RVNAGETYRGRRYVVEGDASSASYFFLAAALLKTTIRVEGISRESRQGDIRLLDVLEK
                     LGCRFRSTTNGVDVTGGTLLKGDRVFDFGDMPDMVPTLAVLSAFREGQTVINNVAHLR
                     IKESNRLAAVATELRRAGIDAREIPDGLVIKGGRMRPAKIETYNDHRMAMSFAIAGLV
                     VPGIDIRDKKCVDKSFPSFWEELRRL"
     gene            109624..110196
                     /locus_tag="PLR13_00525"
     CDS             109624..110196
                     /locus_tag="PLR13_00525"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQC17840.1"
                     /translation="MKVVLIGYRATGKSTVGKMLAARLNVDFRDTDAMIEESMDMPVK
                     EIVALHGWEYFRSKERDIIKSLVKKEDCVIATGGGVVLFRENVDLLKQNVDLLKQASV
                     MVWLNAPLRDIVERLKQDAQNEATRPPLTSGNIVQETIDTMRKRFPLYERSADYVVDT
                     LSKTPEQITEEIYQYLLKSGNLAKITVIKK"
     gene            110208..111284
                     /gene="aroC"
                     /locus_tag="PLR13_00530"
     CDS             110208..111284
                     /gene="aroC"
                     /locus_tag="PLR13_00530"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQC17841.1"
                     /translation="MSGNTTGSVFRVTTWGESHGRALGAVVDGCLPGIPLTESDIQQA
                     LDRRKPSASVASTTRKEGDLVEILSGVFEGKTIGTPISLIIRNADAQSSSYDALKNVF
                     RPGQGDFTYLQKYGIRDWRGGGRASGRETAARVAAGAIAAKILDRVGIDVIAYTKAIG
                     SIAIDRGRMAAEKKLRTAVQENALFCPDLQAAARMERKITAVRKKGDSLGGIVEIIVR
                     GCPGGLGEPVFDKMDADLAKALMSIGSVKAVEVGDGFSMAGLQGSQTNDEIMPGGFKT
                     NHAGGILAGITNGEHIVLRAYCKPIPSIAKPQRTIDSKGKPRFIEIQGRHDICVLPRI
                     VPVCEAMVNIVVADHFLRQRAIQS"
     gene            111281..112066
                     /locus_tag="PLR13_00535"
     CDS             111281..112066
                     /locus_tag="PLR13_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQC17842.1"
                     /translation="MKKINVGIIGGTNGMGKWFADLLKKEGCRVYVCGRKTRLHINDL
                     AGLCDVVVVAVPISATPEVIGQVGPQLTKDQLLMDLTSLKKEPVTRMLKTSQAEVIGC
                     HPLFGPQLKDVSGQNVILCPARGKKWLGWLKGVFKRSKMAVRESTPEKHDRMMAVVQA
                     LNHLDTMVLGLAIARTGMAFADIDKFSTPIFRIKMEIIRKVFTESPELYLDIIRGNSQ
                     TIKMLNIYEKALKDIRAVIRTRGKKESTEFVTKVAAKVHGRKN"
     gene            112050..112817
                     /locus_tag="PLR13_00540"
     CDS             112050..112817
                     /locus_tag="PLR13_00540"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQC17843.1"
                     /translation="MEEKINLVAAMIAASKKTVIFTGAGISTESGIPDFRGPDGLWTK
                     VDPEDFTIDRYLRSGETRKKVWYYLVEGGLMTTSQPNRAHLAIAELERMNKLSSIITQ
                     NIDNLHQRAGNDPKKVHELHGNMQWLVCLDCAERYDLEMMRQKHPSPDHFPICEKCAG
                     LLKPGVVFFGEMLPPDTLRMAEQESEKCDLFMVIGSSLVVYPAAYMPLYAKQSGAKIV
                     IVNMGQTGQNDIADVFINAPAGDTLSRIVARLKEIMT"
     gene            112840..113610
                     /locus_tag="PLR13_00545"
     CDS             112840..113610
                     /locus_tag="PLR13_00545"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HQC17844.1"
                     /translation="MCLIVFAYNVHPSYRLVLAANRDEFYERPSSPAGFWKDHPRMLA
                     GRDLKSGGTWLGITKDGRFAAVTNYRDPSSLKSNALSRGLLVSSYLNGNKDPGNYLKK
                     IASDIFNYNGFNLLLGDKRDVFVFSSRGKAQKLKPGIYGLSNHLLNSPWPKVSRSKRM
                     LKTALMQKGDALEDALFSMLADRRIPPDQKLPSTGIGLEWERLLSSIFIESPVYGTRS
                     STVLLIGKNRHIRFAEKVFDGQKDPWMESRFSFIVKLK"
     gene            113833..114585
                     /locus_tag="PLR13_00550"
     CDS             113833..114585
                     /locus_tag="PLR13_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQC17845.1"
                     /translation="MKVVYHEDYNKVYSSDPASALGRIQAVEKILCGKVTFVEPAAAS
                     REDILSVHTPDHVRAVEREGVYDIAALAAGGAIKAAQIGLTEPCFALIRPPGHHASSG
                     CAWGFCYFNNMSIALEKLKREGQIRKAFILDFDLHFGDGNVNILGGKDYVTILNPTGS
                     NRNDYLKQVQNGLAETDADMIAVSAGFDHHEDDWGGLLKTEDYQYMGGLVREAARRNN
                     GGCFGILEGGYNHTVLGKNVLAFVEGLAEKDL"
     gene            114729..116225
                     /locus_tag="PLR13_00555"
     CDS             114729..116225
                     /locus_tag="PLR13_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQC17846.1"
                     /translation="MAGFAEYDQYDGLGLAALVREKKVTPRELCEEAIRCIEKLNPEL
                     NAVVTPMFDFGRDAAEKNTADGPFAGVPFLLKDLISAFAGVRLTWGCRAYKDYIPDYD
                     SELVKRFKKAGLITLGKTNTPEFGLMGITEPELFGPTRNPWNTERTSGGSSGGSAAAV
                     ASGMVPIASGGDGGGSIRIPACYCGLVGLKPTRGRTPTGPKYGRLWQGAVVEHVLTRS
                     VRDSAAMLDATLGDEPGSPYDIKPPERPYSEEINRAPGSLKIAFNTESPLGTGTHESC
                     ADAVIKTAKLLENLGHNVEEAKPDIDGVKLAHTYFILYFGENAADIKWAEAVLGKKVG
                     RKDFEITTWFFGELGRCYSAGDFVAAIREWDVAARVMGQFHLDYDLYLTPTVAYPAAK
                     IGELMPKSYEEAAMKIFSVLKLGSLAKVSGMVNQIAIKTLAKTPFTQLANFTGQPAMN
                     IPLHWDEQGMPCGVQFVAPFGDEATLFRLAAQLEKANPWFNRRPSLKL"
     gene            116365..117216
                     /locus_tag="PLR13_00560"
     CDS             116365..117216
                     /locus_tag="PLR13_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQC17847.1"
                     /translation="MLSEKNNSRFGGASKYILDDELAKIVNVSMALEMPLLLKGEPGT
                     GKTMLAHAIAESLQMPLIVLNVKSSMKLIEALYQYDTLTRLNDSRFGDSKRDVSDIEA
                     YIKMGKIGQAFTADKKTVLLIDEIDKADTDFQDDMLDVLDQMQFDIIEIDKTIRAKNR
                     PVIVITSNAKKDLSDPFLGRCNFHHIAFPDPDMMRLIVSVHFPKLDEELMKICISTFY
                     RLRELRGVEKKPATRELINWIRALKSDPDFKPKTLYQGRVPFLGVLFKKSADLQQAAQ
                     QVIKSRG"
     gene            117227..>118265
                     /locus_tag="PLR13_00565"
     CDS             117227..>118265
                     /locus_tag="PLR13_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17848.1"
                     /translation="MFVNFFYTLKDRGIPVTPTSFLRLQNAMSKGLVHSLDDFYTVAR
                     TILVKSERYFDTYDQIFAHHFRNASLEDPTAVELNEVARAMLEEWLQNPQELADMLGI
                     DKDKLKKMTPEELIQYFLERLKEQTEAHHGGNKWIGTGGTSPTGHSGYHPGGMRVGGE
                     SRNHSAIKVALDRRYRDYSQEGPLTQSQIGEALRRLRRLMPTGPKDVVNVDKTIYETM
                     RNAGEIDIIFDRRLTDRLKVMLFIDNGGWSMEPYIGVVQTLFHYASSQFKDLKIFFFH
                     NTIYSRVWADPQRYYKPQDVDELIRWDPETRVIIVGDASMAPYELMHPNGAIYVDAKP
                     SGTSIERLRFIA"
BASE COUNT        30204 a        27338 c        30881 g        29242 t
ORIGIN      
        1 gtgccaatat ttatttaccc agagggagtg cttgtttgga ttcaaaacct ctttacaaca
       61 gccgaattgt gaattcctat gttaagtata taaaaaagtg ttacgaatat ataaacatag
      121 atgagctcta tgattatgct ggaatagagc catacgaagt tgccgatcaa aatcactggt
      181 tttctcagga acaaatcaat cgtttccatg aaaaacttga cgaactaacc ggtgcaagca
      241 atttagcgcg ggaagccggc cgctatgccg catctccaga atcaataggt gtcatgaggc
      301 agtacgtttt gggaatggcg aatcccggta cggtctataa aatgatcggt aaagtgacca
      361 gccagttttc acgttcctcg atttacgaat ccagagaaat aaatccccaa aaaattgaaa
      421 tcactgttac cccaaaagag ggagttcaag aacaaccttt tcagtgtgaa aacaggatgg
      481 gtcatttcga gtcaatatcg ctggttttta ataacgctat ccctgagata gaacatcccg
      541 aatgtatgtt taaagggggt gattgttgtc gatatatcat atcatggggg aacaaccgat
      601 cgtatctgtt aaaaagaaca agaaatatca tagcgatagt tatggctata gtcgccatta
      661 tcctgacaat aatctatccg gtagatgagc ttgtatattt cattccgatc ataatcataa
      721 cgattctgat catcaatttt ctaaacgaca aacagacaaa gaaagaactt cgagacagca
      781 tcttgaatct tgaacattcc cgcgacgacc tgattcagca aatggaaatt aattataata
      841 attcgcaaat ggttcacgaa atcggtcagg cgatcaacaa atacatcaat ataaatgaaa
      901 tccttgataa tgtaatacaa gtactggaaa aaagacttgg atatgacaga tgcgttatcc
      961 ttttttcaaa cgaagacgca accaggcttg ttttcaaggc gggttacggg tatacagaaa
     1021 aacaaatggc cattgtcaaa tcaacggaat ttgacttaac aaagcctcaa tcaaaaggag
     1081 tgtttgtaac ttcttttcgc gagcaaaaac catacctgat caatgacatc agtacgatta
     1141 cagggaagtt atcacagaaa agtctgcagt tcgtcaagga catgggagct cagtccttta
     1201 tctgctgtcc tattctttcc gatacaaagt ccgtcggtat tctgactgtt gataatttca
     1261 agactcagaa gacacttgtc gaaagtgatc tccgtttgct tatgggcatt gcctccgtgc
     1321 tcggcgtcag catccgaaac gcggaattgc atgaacagcg caaccgtcaa ttgaaatcta
     1381 ttctgaaagc tctggcggca agtattgacg cacgcgatcc ctatacggcg gggcattcgg
     1441 aaaaagtaac cgaatacgcg gtgggtatat gtaaggaaat gaatatgccg tctgattaca
     1501 cggaggttgt tgccgttgcc gcgtcgctgc atgattacgg taaaatcggc atatcagatg
     1561 ctttgttgaa gaaagaaggc cttctgaccg ctgaggaata cgaaacggtt aaaacccatt
     1621 ctgtgaaaac cagaaaaata ctggaaaaaa tcgagtttca cgggccatat agtcttgtgc
     1681 ctgaaatagc tgaagcgcat catgaaaaac ttgatggcag cggttatccg cgcggtttga
     1741 aggggcatga aatacccatt ggttcgagaa tcattgcagt ggctgatttt tttgaggcga
     1801 ttacggcaaa acggttatac cgtgatccta taccagtgga tgaagccttt gaaatgttgc
     1861 gcagggaaag tgatgttcat ttcagtcgtg aaattgttga agcctttata aaattttata
     1921 catcgaacaa catggcctcg caaaaaagta tgtaaaaacg aattctcttc cccagatgtg
     1981 aaaaataatc ttaagatgag aaataattat aaaatgaatc aggtgattta atgacacaga
     2041 cacaaacgta tttgatggaa aacagcgatg aggaaaaacg tctggatatt aaaacagatc
     2101 cggatgcggt taaagagcag gcgttgtggt ttggaatggg gcccggtgct cgagtgctgg
     2161 atgtgggatg cggtcccggt aaaacgacgg cgctgctaca tgatgtagtc cagcctgacg
     2221 gggaagccgt aggcgtggat attgctgaaa gccgcattct ttatgcacgt tcattctacg
     2281 gctatagaaa agggataaaa ttcgatgtcc gggatgtccg tcaacccatg gcagatttgg
     2341 gaagattcga ttttatctgg gtacgctttg ttctggaata ttatcgcgaa ggggcttttg
     2401 atatcatcaa aaacataagc gccaatttga agcccggcgg ttacatgtgc cttctggatc
     2461 ttgatcataa ttgtctttgt cattgggaaa tgcctcggga gatggaagac gtggttatca
     2521 aggcaatgct gcatatgcag aattgcttta attttgatct ctatgcggga cgcaaatgtt
     2581 atgctcatct gtatgacctg ggttttaaag atctaacgct gaatgttgtg gcgcatcatc
     2641 ttatttatgg caaacttcaa cattcggacg aattcaattg gatgaagaaa ctggaaatcg
     2701 gcgctctgaa agcgccggaa atatttgagt cttacgcagg gggcagtaaa aaatttttag
     2761 atgattttag aatttttttc agagatccac ggcgtttcac atattctcca atgattctgt
     2821 gcagaggtag aaagcctttg tgagaagtcg cagaggatag agtagacacc cgatgaaaaa
     2881 tcttatcgaa cacaaaaaat aatgatttga aaatgttttg catggaggaa gttcatgatg
     2941 aagtttgttc aaaatttcag acatgattgg cagcaaaata taattaaacg tcctcctcct
     3001 cgacgtattg tcagtatttt aaaaaaagca tggcaacttg aagaatacta tgcgggcatt
     3061 ttcgagaaaa ataattatgc ggtgcgcgga aatgcgggga tatccacggc tgatgcggaa
     3121 tattcagcga ttttgccgtc ctttcagtta gatgtgacgg aagacgccaa tcgtgcggag
     3181 gaaagttacg attcccggaa gttcagattc aaatacaatc tcccctttaa gaaaattgat
     3241 ccccgtgtcg tatcgtcagc gctggcgacg tctccaaaag taaataaaaa aattctaatc
     3301 aatctgatta atcacctgca ttttacaaag gaacaggttt ccattcacat tcgtgatctg
     3361 aacaccggcg atgaaattct ccagcaggga gagccgggtc cttatctcaa tgatacggtg
     3421 accatccgtt ttatcgatcc cggggcattc gatctggagc ggcatcatcc cttgaatctg
     3481 attatagaca accgtaaatc cctgatcgtt tttccgattg atttgcagtc ggtcaatcat
     3541 gaatccattg tttttaagat gcccgatgtg gctcattcct acagcaagcg ccaggccgtg
     3601 cgtcatactt gcgaaggtgt tgacgcggaa attgttcagg gaacgctcaa tgttcatggt
     3661 gttctggaag acatcaaccc ccatgattta agagtatgca tcagagcacc ggaagacatt
     3721 aatcccgcca atcatttgtt cctgaaactc agtcagagcg atcagacgca tttcatgggc
     3781 gagtgtcaga tgcttcgttc cgataatgag ggctcataca ttatccttca gcccgttcat
     3841 cagaatcgtc cggtcttatc cccccgaaaa tacgccgccg accgggtcag gataacgccg
     3901 ccgccggtga tcaatttcat acatccgctt tgcggtcttg ccgttcagaa caaggttgac
     3961 gatatttcag cctccggttt ttccgtaacg gtttccgata atgaatcgct ccttattcca
     4021 ggaatgcttc ttccgcagat gactcttcat ctgccgggaa tgttcaatga tttggtctgc
     4081 gccgctcaag tggtttaccg ccttaaattg aaaaagaaca tggtgaaata cggattttat
     4141 attctggaca tgtcgttgaa cgatcagaaa agactctttg atgccgtaag caaagccgcc
     4201 gacccgcatg tcaaccttac cgggcgagtc aacatggatt ctctctggga gctgtttttt
     4261 gattcgggat tcatctatcc cgacaaatac ggcatcatca gtccgtatac ggaagcgctg
     4321 aaagagacat accgccgcct ttatgaagaa ggtcaggata ttttcacgca tttgacttac
     4381 caggatcaag gacaggttta cggtcatttg tccatggtca aagcatacga aaattcatgg
     4441 atcattcatc atctggctgc gcgtcctcta aaagggaagc ggaccggact taaaaccctc
     4501 aattatttta tcaactattt ggattgcctg ttccgtttgc cggtctcttc caagaccatg
     4561 cgttatacat tttgttacta cagaccggaa aacacattct cggattatta tttcggcggc
     4621 gtttgcaaaa tatttaagcg tcgggaaata tgttcgatgg atcttttcgg ttatatgccg
     4681 atgaccgttg atgcggagaa aaccggcctc ccgtctgatt ggagtttaga gaacttttct
     4741 cgcgatgatc tggcgctcat gcgtgaatat tatagcggca tcggcggggg gatgatgctt
     4801 gatgcttatg ccctggaatg tcgggcggca gaaaccgaat tatccggtca agttcatgaa
     4861 gttctgacgg agaataagaa caatatcatg gacatgtata ataaaatagg actgaaacgt
     4921 gagagcaggg tcctgtccgt gaagcatgac gggcggatca aagcagcttt gattgttgat
     4981 acgtcggatc tgggcgtcaa tatgtccgaa ctcctcaata gtatcaaaat cattgttatt
     5041 gatcatacgc tgccgtggcc ggtattgcag gacgcggtta gtatcgccgg taagatttat
     5101 aatagtgaca gcatcatggt tttaattttt ccctatgcct acctcggaca gcagggagtg
     5161 gaatgcaaaa aacgttacaa tctgtgggtg ctgaacagca acctggacag tcctgaaatg
     5221 gatattatta aagatcgcgc aaaaatcacc aaacggaaat ttattaccga aaaatttatc
     5281 aaagaaatag ccagaaatcg attgaagaga ttgctggctt tgattcagaa acaacagtag
     5341 acgttgtggg acagggaaga atgggacgca ttcgttttta ttctatcagg tggcaatgca
     5401 taaaatgaag atgttatcga ataaaaatgt attgattacc ggcgccgcca gcggaatagg
     5461 tcgtgctacc gctgcggcca tggggctggc gggctgccgg ctgtttctga cagatattaa
     5521 tgctgaaggt ctgaagaaga cggcggatat gatcagcagc gctggtgaca acgtgtgtct
     5581 gtcccgtccg tttgatgttg gcgattatga agccatgcgg gcgtttgcct cggagattca
     5641 tgcggaattc ggttcgctcg atattctggt taatgttgcg ggtgtggcgc tttttgcgaa
     5701 aatagaagac atgcaacata gggactggga gaaaatcatc agaataaatc tgtggggcgt
     5761 cattcacggt ttggaatgct tcgtgccgga gatgattcgt gcagggaaag gcggacatgt
     5821 cgtttcagta tcgtcgacgg caggtcttat cgggcttccc tggcatgtgg cctatgccgc
     5881 cacaaaacat gctctggtgg gtatgtccga agtattgcga tatgatttaa gaaagtacaa
     5941 gatcggagtc agcgttgtct gtccgggtgc cgtccataca gggttagtca atacggcgga
     6001 cattcgcacg gatgatcatg aggcaaccaa taaagcacgc aagatgtttg tgaaaatcgc
     6061 catgacgccg gaaaaagtcg ctgatttgat tattcaggcg atcctgaaaa ataagtacct
     6121 ggtcatcacg tcactggatg taaaattatt ttactttatc aaaaggacat gtttcccttt
     6181 gtatcatctt gtcatgctga tcataaaccg tttcatggat aaggcgctca aaaaataata
     6241 aaaacgtatc tgacggagta tcacgtgatt caatctgaat caaaacattc aaagggcatc
     6301 aattcctggc cagaggacga ccgtccccgg gaaaagcttc tgcggcaggg tgcagaaaca
     6361 ctgagcaatt cggaactgct ggcaattctt ttgcgaacgg gcgtcaaggg tgcaagtgcc
     6421 atcgatctgg ctcgccggat tgtggaaaaa ttcggcacgt tccgtaacat gattcatacg
     6481 gatgtgcgcg aatggaagga atttaaggga ttgggacccg ccaaaatggc gcagattcag
     6541 gccgctctgg aaatcggccg gcgttttcgc gaagtggagg cgctgaaagg aaaacaaaaa
     6601 atagcctcct gctgcgatgt cgtcgaaatc gtcatgccgc agatgcgaga tcgcaaaacg
     6661 gaattattta aagtcctgta tcttaacagc aacaataaga tcatcgacat cagcgacgtt
     6721 gcggaaggca cggtgaatca tgccataccg attgtccggg agatcattca cgctgcattg
     6781 cagaaatttg cagcagccat tatttgtgtt cataatcatc ccagtgccaa catttcgccc
     6841 agtgcggaag atcgaaaatt tacgcaggat ctgctggcgg cgggaaaagt gatgggcatc
     6901 aaagttctgg atcacatcat catcggagac gggaactatt acagctttgc cgaagaagga
     6961 atactgtaaa atcgttaaaa ttacgtccac gaatgtgcgc ccgtttgatt ggactgcaaa
     7021 tcaccacatc gcttgctcga aaaaggatcc tctgcaatga cctattacat cattattgcc
     7081 ttctgtgtcg ttatcctgct ggcgtatttt tttgacgtga catcgcggca aacaaaaatt
     7141 cccggtgttc tgcttctgat tcttacggga atggccatca attccatcga cagttatttt
     7201 aactttgaag tatcacaaat tgatgtgatc ctgccggtca tggggacggt agggctcatc
     7261 ctgattgttc tggaagccag tctcgatttg accctgagca aacaaaaaaa gtccctgatc
     7321 ctcaagtcca tgtcttcagc cattgttctt tttttggtat tcacgggctt gtttgcgctt
     7381 atgttatcct atggtctggg ttacgatgtt aggacctctc tggttaacat tattccagtt
     7441 gccgtcatca gcagtgccgt agcgattcca tcggcggtcg gtttgtcggg aaataaccgg
     7501 gaatttgtca tatatgaaag ttctatctct gatattttcg gtattctggc attcaattat
     7561 gtggtcatca gtaacggtca tcttttttca ggcattctga attttcttct ggaaatcatt
     7621 gtcacattgt ttgcctctgt gttattcagc gcatttctgg ctgtcatttt gcataagatc
     7681 aaccatcatg taaaatacat catcattatg accgcgatta ttcttgtcta tgcgctgtcc
     7741 aggttgcttc atctgccgtc tctgctggtg gtcctgattt ttggcctggt gatgaacaac
     7801 aataatcttt tcgaaaacag catcaccgct cagttcatta attttcaaga cttcaacaca
     7861 gaattgaatg cttttaaaaa tattacgtcg gaaatgacct ttatcgtccg ttccttcttt
     7921 tttgtcctgt tcggatatta tacgagcctt tttgatttgc tgaatttaaa caatttatta
     7981 ttttccgttc tgattgttgc cgcaatatat gcgttacggg ctttgtattt aaagagcatt
     8041 atccggattt caatgacccc gctgctttat tttgcaccgc gaggcctgat aaccatttta
     8101 ctttttttga gcataccgca agccatggcg ctgccctata tgaataccgg cgtggtgacg
     8161 cagatcatat tcattaccat tttaatcatg gcgtacggca acatcgtatt tgggaaaacc
     8221 ggcgaggcca ttccgaccgg cacgccacag gataaaaaaa acttgtcggg atgaggaagg
     8281 tatgcgcata tatttcattg tacgcgccat ggatgcaacc aaaggcgatg aaatcggtcg
     8341 ttgcattgca ggaaatatcc ggtgtttcgc gcaacggtaa taatgatatt gcgccgcata
     8401 cagaaatgcc gctgcaaacg ttacaggtta aataacgcga tcgctgtgtt gacaaaaaac
     8461 ttgcatgtga tatcagctaa acgctctgcg acaacgggca tcgcaatcag tatatttgaa
     8521 aaaggaggtc catgtgatta tcagaaaatc tgccgttgtg gcggtgatgc tcatagccct
     8581 cttcctttta tcgtgcggaa tgagcgaccc gttttcgggt aaatggttca acgacgtgat
     8641 agaaaaggaa atgctgacca taaccaaaag cggcagtgct tataaagtcg ataccgcttt
     8701 aggatctttc ccctgtatca aagaggggga cctgttgaaa tgtcaaatgg gaatcggaga
     8761 tacgatgctc cagattaacg agaatcggtg tctggttatg aaaacaccgg ccggcgttga
     8821 attcaaattc agacgccagg aaaacaaata aagaattaag tcaatcctga aaacgggaga
     8881 acggatgatt tttgaaaatg tggctcttca taacgtagac gggctataca aacacaaaga
     8941 tattgaaggg cttctgttag gacgtattcc cgaacacgtt tccgcacgtc tcagcaaagc
     9001 cggacaaatg atgatgatct gtccttccgg ttcggaaatc cgttttgtca gtgaaacgta
     9061 tcctgtgaaa atcaccctgt ccgtggataa aatcacaaag catctcggcg acggaattat
     9121 taccgatgcc cgggtttttt tcggaacctt tcaaacgagg cagcgttttg tgattaagag
     9181 aatcaaaacc ctgctggaaa tcatccggcc gccgaaattc atcgaactgg cggagaaaat
     9241 tgcgaccgat gcgccgtttt ctccccatgt ctgtagaatc aggttttggg gaacgaccat
     9301 gggcgctccc atccggtttc atggcattga aacggaagga aaaataagac ctccccacgc
     9361 tgaggaattg cccggcctga gctatctggc ctatggaacg tcgcttaccc agggagctta
     9421 tgcttcggaa tcgcatttga gctatccgaa tctggtcggc tgccgtctcg gcgtggacgt
     9481 tatcaatctg ggttcttcct gctcggcata ttgcgaaccg gaaatggccg actatatcgc
     9541 cggaagaaag gactggagct ttgcgacgct tgatctttcc ctgaacatgg tcgacacatt
     9601 ttcgccggaa gaattttcga ggcgagtcag ttacatgatc aacaggatcg ccggcagcaa
     9661 caaaagccgt ccggtttttt gcatcacgct caagccgtat ctgggagatt atatcgaaag
     9721 caaaaagccc gacctgtacc gcaaattgct tcggaaagcc gtggaaaatt ctgcgctgga
     9781 taatctctat ctgatagaag gtcaggacct gatgccggat gttgcgggcg gtctttgccc
     9841 ggacatggtg catctgggag acttcgggac gattcaaata gccgaaaaac tggccgcaaa
     9901 aataaagccg gttctgcaat ctcatcatct tctttttaaa cactcccgat agggattcgt
     9961 tgaatttgcc gccttccccc gtgtccggct ttgttgaaac ggcttccgtt tgataaagaa
    10021 tgtagacacg atgctcataa tacgttaaaa atacttatat tttcattttg tcattgagaa
    10081 cattttgcat ttattgtaag gtgccaatga aagtgctggt ttggggggta ttaaatgtat
    10141 gatcacgaca cttccaaatt tcaatccgtc aaggaattca taaaacacaa ttatcgacac
    10201 ttcaattccg ctgtcgtcgt ggatgcagcg gaggcctacg cgaatcatat taataacggc
    10261 gggaaaatgc tgatggcact ggccggggcc atgagcaccg cggaacttgg tatcactctc
    10321 gcggcaatga ttcgcagcgg taaaattcat gccgtatgct gcacaggcgc aaatctggaa
    10381 gaggatcttt tcaatctggt cgcccacaag cattacaagc gtgttccaaa ttacagggat
    10441 ttgaaacccg aggatgaagc ggaactgctg agaaacaagc tgaaccgggt gacggacacc
    10501 tgcatccccg aagaagaagc catgcggaag gtcgaaggca agcttttcag tttctggaaa
    10561 aaagctgcgg atgagaataa acggtacttc ccctatgaat tcatcatgca gatcatcagc
    10621 agcggcgtgc tgaaagatca gtatgaaatc gatgaaaaag attcatggct ggttgccgcc
    10681 tgtgaaaaaa acattccgat ttttgttccc ggatgggagg actcctctct gggcaattat
    10741 tttgtttcca gtgtccgcag gaaacaactt aagcattatt cgattgtgaa atccggtctg
    10801 gaaacaatgg atgccttgat tgactggtat ttagatgcat cccggcacag cgaaacgggc
    10861 tttttccaga tcgggggagg tattgccgga gatttttcca tttgtgttgt tcccctgatt
    10921 cagcaggaca tgaaaatgga ctgccggtta tggtcctatt tctgtcagat ttcggacagc
    10981 acgacatcat acggctcgta tagcggcgcc gttcctaatg aaaaaatcac ctggggaaag
    11041 atccgccccg attcaaaaaa attcatcatt gaaagcgatg ccacaattgt tgcccctctg
    11101 attttcgctt acgttcttgc ggaataagcg ggattcgccc ggcggttttc aaatttaaaa
    11161 tccggtgatg ttccgggcag gagaaaaacc gatttcccgg gcataggtga attgccgttg
    11221 ccagggaatg ccgggatgga ctttttcgaa aatatctgcg ccggtcattt tgagcgattc
    11281 cgcatccacg ggcacgggat gataaccgcc gataaatccc atatcctcat gaatcaccgg
    11341 attcttaccg gggagacagt cgcattccac ggtaatattc agcagtgaat tgatgacgat
    11401 ggtcttgtcc ttgattattt tccacacggc cgccgccgtt tccacaagct tttcctgaaa
    11461 aaccgtcatg tccgaattcc agaaaatctg cagtgccatt tccggacaca tcgcaataca
    11521 ctgggcgcag ccgatgcatt tttttaaatc atatgtggga tagtcggatg ttgcgatagt
    11581 tgccgctccg gtcggacaga tttccgcgca aactccgcag cgtgtacatt ttttcttatt
    11641 caggacggga tgataatcgg aatgcatgcg ttgcttctgc gcgcgggacg cgaaacccat
    11701 agacatattt ttaatggcgc ctccgaaaag cgaaaccatg tgtcctttga aatgggagaa
    11761 gatgacaaac ccctgcgcgt tctcaaaaac attggccacc tgcacggtcc tgaaatgatt
    11821 gaacccggcg ggaatatcca caagattgcg gctgtccatg ccgtcggcga taatcaccgg
    11881 acaacccaga aagtcatgcg tgtagccgtg ggaggcggcc gttatgagcg agtcttcggc
    11941 cgtacggcgt ccgccggaat agagaaccgt tgtatcaaaa acaaacggtt tagttccgtg
    12001 ggcaattagc caggtagcaa tttcccttgc ataatcaggc ggcaggaaac ctttattgcc
    12061 tttttctccc cagtgcaatt taatgccgac ggtgtcgccg gcggagaagg gaggccggag
    12121 gtgttgaaga agctttctca gcgattcaag gtaaggtttt ttcccgcctt taaccggttc
    12181 aaagaataaa tctttcatgg tccgtctccg ttccttattt ggataacata ttttaaatct
    12241 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatca acaaaccatt
    12361 gcgaaaactg ttatgctgat cccggatttc tcaaaatatt ctctggcttt attcgagggg
    12421 ggcgcgttaa ttcatgcatc aaacggcaag ggggtgcgtc cgcttttcga ggcgcttgaa
    12481 aagttccggg caaaatccgg attgattctg cacgacaaaa tcatcggact ggccgcggcc
    12541 aaactggtcg tctattcggg cattatcgcg aaagtgaaca caacggttgc ttccgctccg
    12601 gcggacagat ttctaaaaga tcatggaatc ctcatgaatg ctgcgggcat cacggccaat
    12661 attctgacgc aggataaaag agcagtctgc cccggtgaag tcatcgcctt gaaaacaaaa
    12721 gatcccgcag atttcatccg acaaatcaaa acgatgctcg ataatcagcg aaaataagta
    12781 caatacctgt acttattcct actctttttt tatttatttt aggaatatta aaaaaatatt
    12841 tgtttttgtc ttttcatagg ccacataaaa tgataatagc caaataccat ttaaatccga
    12901 attcaacgat ttcctgttat tcggaaaacg gtttaattct gcaatgtttg gggctttgcc
    12961 ggatgtcatt ttgtggcaac aagagttgtc cccgaaaatt tataacggta gcatcacgag
    13021 attaaaacgt gaagaatcca gacatgccaa aacgggagaa gaaatattcg gacgatggtt
    13081 agaggatatc tgttggagac atcattatga agatatgtcc tgtttgccag acgcgcttta
    13141 aggataaaac tcaaaaatgc cgttattgca atgttgtgct ggaacccctg acgccagcgt
    13201 cggcatctca accgccgcaa ccggatcagc aggcgaaaca gcctaagatt gtcaaagatt
    13261 cgcggaagaa gagacaaggc aaagctccaa tcgaacgtca aaccggtttc ggaaaaacat
    13321 ggaacagaaa taaatatcct ttcatagtcg tgatcatcct gattgtcggt attctgcttg
    13381 caatcgtaca aaaatgtagt gaaaagaaag ttgaagtgag caacacacag gcggtcgaac
    13441 agcaagccgt ttatgcacct gtaaatcagc cggcagcttc cgataaccga atgatagctg
    13501 ctgcacccgc ccgtgatgag gcgaatggat tatttcgcaa agcatttgaa ctgtgtatca
    13561 gcggaaaatg caccgatccc cggaaggcca ttgaatatct gagcgaagcc attaaattaa
    13621 aaccggatta tgccgatgcc tacaacaatc ggggaaacgc ctacattgaa atcggccagt
    13681 atccggaagc tgttcaagat tataatgaat ccatcaaact gcaaccgcaa tcgatttacg
    13741 gttataacaa caggggattg gctttgagta aactcgggaa acatcaggaa gccattgcag
    13801 attataatga agccatccgc ctgaaaccgg atagtgccat catccacaac aacagaggcg
    13861 ccgcttatct tgctataggt caatatcaga aggctgtcga ggacttcagc gaagctgtta
    13921 aaataaagcc ggattacgtg gatgcctatt acaatagagc caacgtttat tttgatcaca
    13981 tgaataaaga agatggttgc aatgatgcgc aaaaagcatg tgaattagga aaatgcgacc
    14041 tgttggaaaa ggtaaaaggc aagggatact gttcgtgacc ggaaaaaatg tttcagaacg
    14101 tccggtacaa tggttgtgtg ctgcataatt ttttttatct gttcatcgaa tttattgtcc
    14161 aacaaaatct aaatagacaa aaatattttt aaattttaac cttttattcc gtgatgaagg
    14221 tctttccaca atattgtgga aagacctgtt gataaccctg ttaactggcg ccataagtgt
    14281 tcaattatta cgaatttcca ccaatatgca caaggtttat gcaataataa tatttaataa
    14341 tatcaatatc ttacattttt accgtttggg ttataatcgt ttttacggaa aaatcgtttt
    14401 acgataaaca tatttcgttt tttatagctg tgaaatttgc aaattttcaa attatttgcg
    14461 tttttttaat tcgtaataaa aggaaaaccg gatgaggatt ccaattgctg tcaagatgaa
    14521 tgtaactgat ggaaagataa caattatgaa ggttaaaagg gtacatcatt ttcttcaaga
    14581 tttacgtcaa acgtattgag gaaaatgctg accgccatgt agcagcatac cgcgatagaa
    14641 agttctacca gtaaatcctc agaaaaatgt ctcgagattt cggcgaacgt tgcatcgctg
    14701 acctctcgct gcaggacggt ttcttcggtg aagcgcaaaa cgagcttttc gatgccggaa
    14761 aagaccgggg atgctgaacc ggtatccaga gctttaattt tttcttcggg caagcccacc
    14821 agtttgctga gtttgcgatg ctgatgcacc tcatacgcgg aattgcacag aatgccggcg
    14881 cgggtgatgg ccatttctct cagaaccgga tcgagtttcc ccgccatgag cacagatgcc
    14941 ggaagtccga taaactccct ggcaatcccg acgggggcgt gcgtgatgat cttgaaaata
    15001 ttcaaatcaa aagggggttt gccctgaaga aatttctttt ccgtatcagc cggacccggt
    15061 tgaaaatcag gtatgcgtgc caaggcgatc tcctttcagc tcatcggttt tttcgtgaaa
    15121 tgacgtttca tgccgttcat gacggtaaca cagcggttca tttcgcgata agcgggcagg
    15181 cctgcgtttt cagcaatacc ccggatatgt tcggcgaaat cctttcttcc ttcgatccat
    15241 aacacagccg gttttttgtt tttcttaatc atcgcagcaa gttcttcaaa aaaatcctga
    15301 ccgatcaatg gcgaggcata actgtgaaag atgattgaat cgacccccgg atcgttgatg
    15361 accgccgtca gagaattccg gaataccttt tcgaagccgt ggcgttcgat ggcgatccag
    15421 agatcgagcg gatggtcggg tttattccag ggcgggaaaa gttccgcgac cgccgcgagg
    15481 gtccggtcgc tcaattttgc cagtttcaga ccgcagtctt ccatcaggtc tgtggtgacg
    15541 gttcccgcgc cgccggagaa cgttaggata gccgtgccct tttccttttc gggatgaaaa
    15601 ggcatgttct tggcgaaagc ccgtgcgatg tcggtcagtt ccgccggatc aaacacttcg
    15661 ataatgcccg cctgtctgaa ggcggcgctg gcaattttat aatttcccga caggcttgcc
    15721 gtgtggcttt gcgccgcctt tgctccttcc tggctgcggc cgcccatcag aacaataatc
    15781 ggtttttctg tcttctttgc caggtccaaa aactttcttc cgtccaccag cgattcgaga
    15841 tagcaggcaa tgacctcagt atccttgtcg tcaatgagat gttccaggat gtcgttttcg
    15901 ttgatgtcgc acttattgcc gatggaacac accttgctga cgcccatgat gccttcctgc
    15961 agggcatgca gcaggaaacc ggcggaaagc attccgcttt gcacaatcat gccgacgttg
    16021 ccgccgcgca aaacgtccgg ccagaccaga gaattgataa aagaaaaaac ataccttttg
    16081 ttggcatcaa taaagcccat gcagttaggg ccccacaacc ggatgcccgc tcgggcggca
    16141 ttatccaggg caagtttctg aattctggcg ccttctgctc cggcttcatt gaatccgcct
    16201 gattcgatga tgatgccctt gacgcctttt tttgcgcatt cattgaccgt gtcgggcaat
    16261 tctttcgaag gaataaaata aatagccagg tcgatattgc ccggaattga agcaaggtcc
    16321 ttatagcagg gaagtcccag catgtccggg taatttttgt tgaccggata gattttcccg
    16381 ggaaatccgg acgtaatatt tttaatgata tgatagccgc ccttattcat attgctggat
    16441 gctccgataa tggcgacgct tttcggatta aaaaaataat ctaacattac attctcccaa
    16501 atcttcgttt tatgcattgc tggcacacgt gcagggcaat gaattgtcat gttgagaaca
    16561 gggaccaaaa tatttttgga ttgtcacgat gttcaaaaag tgttctgtaa tgacagcgtt
    16621 acttcaatac caccagcgca tcgacggcaa ccggtttgtc tccgcagaca atcagcggat
    16681 tgatatcgat ctcggcgatg gcttcatact tgatggccag atccccaatg cccacaagcg
    16741 ctttggccag agcatttcgg tccacggccg ggctgccccg gaattcgccg agtaattttt
    16801 tagttttgat ttcatcgatc atttccagcg catcctgttt cgtcagcggg gcaacccgaa
    16861 acgtcacatc cttgagcgct tcggtaaaaa taccgccgag gccgaacatc acacaggggc
    16921 cgaaactcga atcctgcgaa agaccgatca ccagttcgcg cgaccccctg accatctttt
    16981 ccaccagcac gccgtcgaga ttttttacct tgccggtcag tgcgtcgaaa acctgtgcga
    17041 cgtcctgcgc gctgttgaca ttcagtgcaa ccatccccgc ttccgtttta tgggaaagtt
    17101 cggcggaaca ccctttcatc gccacgggat accccatttg ttcggcttcc cggatcgctt
    17161 cttctttggt tttggtcagc gctgctaccg ccaccgcaac accggctgat tcaattacct
    17221 gcctggattc gtattccgaa agagtctttc gtccttcctt caaagctttt tctattacat
    17281 ccaacatagt atctccttat catttaacgt taaatcatta gtttatttta tccggaattg
    17341 tcaagaacct ttattgagcg caactggcgg ttgacgaatg atcgacaaaa ttgtaacaaa
    17401 actttttaag gttccgggga gcataactat tgcagcaatg ttatggcgtc agaaaaaata
    17461 ataagcggca tgttaatggt ttcattcccg actaatggtt gaagggatgg ttgagagatt
    17521 atgacgggaa acttcaattt ttatatgctg atgtccattg ccgcctacat ggtggtggtc
    17581 atcgtcatcg gctttgtctt tgccagacgc aatgatcaga tcggcgattt ttatctgggc
    17641 ggcagaaaac ttgggaccat cgtcactgcc atgagcgccg aagcatcaga tatgagcagc
    17701 tggcttctca tgggacttcc cggcgttgca tatctgtccg gtattgcgga tgcgggttgg
    17761 acggcaatcg gtctggcgat cggcacttat gtcaactggc ttacggtagc ccgtcgtctg
    17821 cgctgctatt ctcaactgtc gcaaaacgcc atcaccctgc cggaattttt ctccaaccgt
    17881 tatcgcgatt ccaaaaaaat tctcacatgg atcgccagca ttgtcatctt gattttcttt
    17941 attccgtata ccgctgccgg ttttgcctcc tgcggaaagc ttttcaatag tgtgttcggt
    18001 cttgattaca caacggccat gctcatcggt gcggttgttg tcgttgccta tacgacggtt
    18061 ggcggatttc ttgccgcaag cactactgat ctgatccagg gcattgtcat gagcattgcg
    18121 ctgttgagca ttttgatgtt cggcgctgct gttgcaggcg gcattgatgc cgtgtggcaa
    18181 aaggcggatg ctattcccgg ttatctcagt atgatgtttt cacataattc cacgaccggt
    18241 cagacggaac gctacggact gattaccatc atttctacaa tggcctgggg actgggttac
    18301 ttcggcatgc cccatattct tttgcgattc atggccatcc gcgatgaaaa gaaaattgtt
    18361 ttttcacggc agatcgcggt gacctgggtt gtggtttcac ttgccgtggc cgttttgatc
    18421 ggcattgtcg gtcaaaccct gtccctcaac ggtgagattg ccgcactgga aggaagcgca
    18481 tccgaaacaa tcatcatccg gatggcacag cttctgagcc acaccggcgt gatcagctct
    18541 ttaattgccg gtgttgttct ttccggcatt ctggccgcca ccatgtccac ctccgactca
    18601 cagcttctgg tggcctcctc cagcgtgact gagaatctca taaaggaatg tttcgacatt
    18661 aggctttccg aaaaaaccgg catgatgatt gccagatcgg ccgttattgt gattgctgcc
    18721 attgccgcgt tcatcgccag agatccggac agctcgattt ttgtcattat ttcgtttgcg
    18781 tgggccggtt tcggcgcggc cttcggtccg gttatgctgt ttgccctttt ctggagacgt
    18841 tccaatgtat acggcgccat agccggcatg gtttccggag gcgtgatggt ctttgtatgg
    18901 aaatatctcg tacggcccct cggcggcgtt tggaacattt atgagctgct gcccgctttt
    18961 gttttcgcct gtttggcaat catcgtcgtc agcctgctca gcaaagcgcc tgccagagaa
    19021 atcaccgacg agttcgacgc cgtcgtcaaa atgggaaacc ggaagtaatt ctgacgttag
    19081 cctgcatcag ccgcgaatat ggaatgcacg gcaggaatct ggtataattt tgcaatgaag
    19141 aaagcaacaa aagaaagctg gacgcaaaaa gtcaaggaaa ccagctttgc cctcgatttg
    19201 caatccggtg tatttacttt tgacaatccg cgacggattg ccgaatcgct taaagaatcc
    19261 gccgagagct ctacgcaaag aaaagcggaa ccttttcaat ccgccatgtc catgctgaac
    19321 tattatatca atcgcgcggg cacaaaactg ccggccgaac gaaaagcaat attggaacag
    19381 gccaaagacg aactgcgtgt gttgttcggc agaggcaaca agagtaaaac atctttaaaa
    19441 ataaagaaat aaacggaagt tgaaaaatga attctgaaaa tattccggtg attatcgggg
    19501 cagggcaggt gacaaaaaga gaggcatcat ctgtgcaagg cggttcgccg atagacctta
    19561 tggcagcggc cgtgaaaatt gcggcagaag atggcggcgt gacagaaaaa tacttacaac
    19621 aggccgacct gctgatgacc acgtcgcttt tttccgatga cggtattgcc aacccgccgg
    19681 gatgcgtggc cgataaactg aatctgaccg acgcacgatg cagagtttcc ggctttggcg
    19741 gtgtcatgcc gcacgccgtg ctgcatcatg ccatgcagga gattgccgga gggaaagccg
    19801 gtctggttat tttgaccgga gcagaggctc aacacaccat gcagcaggcg aaaaattcca
    19861 aaacctttac cggctggaac gtggcgtccg gtcggccctg cctgccgcca ctgactccgc
    19921 tggggctgtt tgacggcgcc tgcgaaacag agcacattca cggactttca cttcccgctt
    19981 atgtctatcc catgtttgag aatgctcttc gccggcgcta tggctgcaca attgaagagc
    20041 atgcagaaaa gataggcagg ctgatgagcc gcctctcctt ggtggctgcc aaaaatcctc
    20101 ttgcctggtt tcagcattcg ttcacgccgg aagaaatcat gaccctttcg gaaacgaatc
    20161 gcaacaccgc tttcccgtat accaaacgga tgaatgccat gctgttggta aatcaggccg
    20221 ccgcactcct tgtcgcatcg gaagcacagg cacaaaagat gggcgtgaat ccgttaaagt
    20281 gggtgtatgt ccacggttat gccgaagcca acgatcactg gaatgtcttg gaaagagaag
    20341 gctatggttt ttctcccgcc attgaaatcg caggccggac ggccctgaag caggccggtt
    20401 taaccatcga agatatcgat ttctttgatc tgtacagttg ttttccggtt gccgtgcagg
    20461 taacccgaga catgctgggt attcctgaaa atgacagccg cgatctgacc gttacagggg
    20521 gacttcccta tttcggcgga ccgggaaaca actatgtcat gcactccatg gcgcaaatgg
    20581 ttgaggttct gcgccggcat cccggcagga ccggtcttgt cacgggaaat tctttttaca
    20641 tgaccaagca ctcaacagcc gtgtgctcta cccggtctcc cgagaacaat gcggcggcga
    20701 cagccgatac acgaacgtgc cagcaggcag tcgataaacg gcctaaatat gagattgatc
    20761 cgacgccttc ggggcgggca acggtggaca cctataccgt gatttacgac cgggacaatt
    20821 tgccgaagaa gggtattatc atcggaaaag aagaaaacgg gaaacgcttt gccgctttta
    20881 caccttcaga tccgtctctg ttttcagcca tgattgagaa agacttctgc ggtgtaacgg
    20941 gcagagtcgt ttccaaggat aagatcaatc ttttcacacc ggactgaaaa cgaagattct
    21001 gaaatcatca tcctgacgtc tgcggtcatc atatccgcag cgaccatgca tccgaatcaa
    21061 actgatagcg cagaagatag accttgccgt ctgcggtcct tactttgaag aaattaataa
    21121 cgggtttgct gctgtacaga cctccgtgat accagcgatc aacaacctcc tgaatttcct
    21181 gccgctctcc ctgatatgta aaagcaaccg ggcgttcatt cgccttaaaa ccgctgtagc
    21241 attcaacctg aatttcttga tatttcccat tcatgtttta ttgaacgttg aacttagaac
    21301 gctatcacgt aagcttcacc acgttgggtt tcaccagtct ttcataatcc ggataagaca
    21361 tctcgaccag ttccgagtgc gaacaggcgt tgaaaaagat ttcatcatcg tccgtcagat
    21421 gctgatccgc gtaaacctcc atcccgtaca aattgccgaa aggcggcatg gcgccgagct
    21481 cgcaatcagg gaacatgcct ttgaattgcg cttcggtggc aagttcgatc ttcttcgcgc
    21541 cgacggcttt ttgaaaatga tcgaaattga cggagcgatt gcccggcagg accgccattg
    21601 ccatctttcc gtcaatcatc acgatgacgg tctttgccac cttccttccg ctgatgtgca
    21661 gggattgagc aatttcgtgg gctgtgtacg ccgtcgaatg cgtgagcttc acatatttca
    21721 ctccatggct gtccagaaac tctttcaatt ttttggccgg cataagtgac ctcctttgaa
    21781 ttgtgttgac ggggttcaat gcttccgcaa acagaccagt caaaagagcg ggatcaagtt
    21841 ttctgagcag gagagggaat caggaacatc tgtatcgaac ttttttatat tttaccacgt
    21901 tccacttaaa attgtcattt tttttaattc ttaaacaagg aacgttgagc gaagaacatt
    21961 caacgatttt tttaagttca tttcagccgt gcgccatcct tggccaacat ttcccgaagc
    22021 ttgccggtgt caacggcatc aatcgattgt cccgacgaaa gagccagcgc gcatgccgtt
    22081 cccgccgcct gtccttccgc aaggcacggg cccatcacac gcgccgaggc cagcgcgccg
    22141 cgatccatcg aaatcgctct gccggtaacc agcaggttgt tgatttcccg gcataggaac
    22201 gaccggtaca agctgccgta ataacgccct tcggggagaa agaccatttt gggttctttg
    22261 cccccactac caccagcaac tcaaattacc ataactgata attcaactac gagtttttgt
    22321 gtgctactct tctgaaacca gggtttcatt aattacttga ctgaaaggaa cggacaaacc
    22381 ataattaaat gtagtatggg tttttaaaac cgcaactgca agttccatct caggagggct
    22441 tatgaacgac aatctcattt ttcaggaaac gcccgacctt atcaatccct atattctaat
    22501 cggttaccgc ggctggctca atgccggtga gatggcgacc ggaagcatcg actatctgcg
    22561 ccgcaaactc gatgcccgaa agttcgcttc cattgatatc cggaatttct atatctggca
    22621 ggtaccggga ttcgatccgg cgcaggtcat gcgtccgcat gcggtcgttg aaagaggcct
    22681 gatcaaatcg ctcaacgagc ccgccaatga attttatttc tggaagagcg gtaaagagaa
    22741 cgacctgatt cttttttccg gttttgaacc gaacgtcgca tggccggaat tcacatcggc
    22801 tgttttgaac atcgccaaac gttatcaggc gtgtcggatc tatgttctgg gcggcgtctt
    22861 tgaccaggtt ccccacaccc gggaaacaat cgtttatgcc gtgttgagcc gtccggagat
    22921 gaagcatgag tttcaatcat tcccgctcct gaattacacg gggccatgca gcttttcgac
    22981 gctgctggtg aatcatgccg gcagggaagg gatagaggcg gccagcattg tcgccagagt
    23041 cccgccgtat attgaaacgt ttaatgcaaa aataggctgc gagtatttga aaaaagttct
    23101 ttcccgcaca tccctgaaca tcgatttaag tgatctgaaa aaatccggag acgccgttaa
    23161 caaaatgatg gaaaatgatt tcaggcagaa tctggaggct ctggtgcaac tgaggcaact
    23221 ggaacagatg tatgacgaat cgcttatgca gggatacggc gaaaacccgg aagtggacat
    23281 cgatgagttg atgcaggcca tgcatgagat gaaaaaggat atcccgaaaa aacactgatg
    23341 tcccgtttcc cttcttctga acgttggaga ttgaacatga aacgacattt caaacatatg
    23401 tcatttggag acatgcgaca tgcatatacc gccggttttt gtatatccgg aagctgttct
    23461 tttttggacg gcgttaatcg gatcgtttta tctggaaatt aaatttgccg gcgcccttgc
    23521 cagaaatcct tccaacacgc aggatgccgg gacccgtctt ttaatcaaaa tcggttatcg
    23581 cgttgctttt gttctggcgt taatggcatc gctgacaccc tggtttgtca tcacatggca
    23641 gcgtccggca ctggccgttg gaacgggcat gctcattgca ggcagtatat ttcgtcaata
    23701 ttccattcgt gctctgggaa aatactttac tccggaggtt gttgtaaagg cggatcagcc
    23761 ggttattgaa gaaggccctt accgctggat tcgccatccg gcatacagcg gagcttttat
    23821 catgtttgcg gggatcggta tggccctggg caactggttg agttttgccg ttcttttcct
    23881 ggagaattgt tttgtctaca gccttagagt aaaagcagag gaaaaagctc tgatcgatac
    23941 gattggcgga ccgtatcgcg tatatatgac acgaaccaaa cgcttcatcc cgttcatttt
    24001 ttaagcgaga atatatttgc ctcctgaaaa tgacgcaacc gtgcgtaaat gaaatgtcgt
    24061 caggattagt tatgagggat tcgcaatgac ggatattgat cgtgtacgtt gacaatcgac
    24121 aggcttgttt atataaaaaa attatatcaa accggacgag gtgctttttc atgaaggaaa
    24181 ccggcaggaa tcaggcgtca aaagaaatta aggtgctgaa aaataaaatt aaagagctgg
    24241 aaaatctcct gaacacgaca aaagtcgggc agacacggat gtcaaccggc ctggtctatc
    24301 gcacgatatt tcgcatgtct cccaatacca tcgttgtttc aaaactggag gacggcacca
    24361 tctatgatgt gagtgattct ttctgcgaga aatcagggtt tgcaagaaaa caggtcatcg
    24421 gcaagagggc ttcaacactg ggcatatggc tcgatcagga tcgtgaagaa ataaaaaaac
    24481 gtcttctgag aaacggaaga tacaggaata tggaagtgcg tcatcaggtc aaaggcggca
    24541 aaatgcttca atgtctccaa tcggcagaac tgattaccat tgatgatgaa cgctacatta
    24601 ttacagtcgt ggttgacatt accgagcgta aaatgatgga agaaaggttg cgcgagaacg
    24661 aagaaaaata cagacaactt tttgataatt cgcctgccgg aatatatcag atcgattatc
    24721 aaaccggaag attcctgaaa gcgaatgatg ttttttgtaa atatgtcggc tgtagtcagg
    24781 aagaggtaac gtctcttaca gcatttgaca ttcttacgga agaaagcaaa aaaagatttt
    24841 tggaacgcgt ggaaagattg tctcaaaatt tggaagttcc ggactcggtg gaatttgaaa
    24901 tattgaataa aaaaggcgag tcgttttata ttcaactgca aattagaaat gtttgcgatg
    24961 tgaaaggccg tgtggttgct gccgatgttg ttgcgcatga catcacagag cgcaagcagg
    25021 cagaggatga gcttaaaagt tttgctgaag atctggaaga ggctaatatc acgctgcgtg
    25081 tgttgatgaa tcaacgggat gaagacaaac aggaaatcga agaaaagttg cagaccaaca
    25141 tgcgtgatct cgtaatgcct tatttgagaa aactgaaacg tttgaacctt gacggacgca
    25201 gtaaaaatta tctggacatt ctggaaagca atctaaacga ggttttatct ccgtttatga
    25261 aaaatctcca atcctccttc aatcatctta ctcctcagga aatgcagatt gtcagcctga
    25321 tcaaaaaggg gaagaaaaca aaagaaattg ctgagatgct caacgcatca accaagacga
    25381 ttgcgaccca tcgaaacaat ctaagaaaaa aattgaatct gagaaattcc aaaatcaatc
    25441 ttcgctcgta tcttctatcc agcgggtaag ccgttaaaga acatatcgtt ttgttcgatc
    25501 cgaacggtgg atgtatcaaa atattgacgc ggcgtccgct tggcggccca tccgctgcga
    25561 acggatccgc ggccgtatgt ttttttccgg tagtgtgtat gtctgttttt tttaattcgt
    25621 tattgtgtta gtatagtacc aaagaaattt tcttttttca gaggtgcatc gtgatcaaaa
    25681 caatgcgttg ggttatgctt ctcacattaa tgatcttctg tcatccggca agtcctgtat
    25741 tcggcgcttc cgaatggatt gaattttaca cgggcaatct ggggaatgtt gctttttaca
    25801 aaaaagtcgt ttctaaagac ggcgccgtga aatatgtcat taaagatgtt ttttccgata
    25861 aaggcagaga ggcgtttatc cgggacagag ccaaaaaaaa actttccacc gtggaatatg
    25921 aagaaatatc gaatgttcaa tccttgaatg aaatagattg cagagaaaag aaaatcagac
    25981 atgtctccat tttttatttt gacaaggacg gtaagctctt cagctccgat gtcattgata
    26041 atccaaagtg ggttaagata cccgataatc cgttcttcaa tgccctcata aaaaaggcat
    26101 gtcaataagt gatgaatttt atcaaagtaa gggtttttaa gtaagcgccg attaatgatt
    26161 tttctgatca attgttttgc cgtccgataa atcccctgcc cgtagtaatg attcttcata
    26221 aacaggtaca tccttaaaaa gatacaatcg tctcacgcga cagatattat gcaggcaagg
    26281 tccgcacgat gaggaatgat ttgcatattt tttgatgatc cggatatgag ggcaaatcct
    26341 tgccgtgatg ataaggattt gtaatgatta acgaaggttt attacggaat gttttctgtt
    26401 tgccttttat gaataatgag tggataataa atatccggct cggatattga gatgggtcca
    26461 tcgtgcccaa atatggcggg acaaggtaat gatgcaggag aaaaagaaaa aaatacgctg
    26521 ggacatttcc atttgggcgg ctgtcagcgc gattaccgtt ttgatggtga ttctgggcat
    26581 gatgatgttc atgcagtttc agcagagcga gacacaggct caggcgcttt ttatcgaaaa
    26641 gggcgcaacg ctgattcaat cttttgaggc gggattgcaa gatcgtgtgg atggcaaaaa
    26701 cgatcgtttt tatttccaga aactgctgat ggcaacggcg gagcagccgg atatcgatta
    26761 catgatcgtc acggacagca agggaaagat tctggcggac agcgatccct ccatgctgga
    26821 tcagcagtat ggcatagatc tggatacgga aaagattgcc cgaagcaagg acatgcactg
    26881 gcgtcaggta accaatcccg gcggcgcggg aacatttgaa atctaccggg gattgttccc
    26941 gtggcggcaa acagacgcgg cgttacatca tcgttcgctg attgtgtttg tgggattcaa
    27001 catggataag atcgaaaagg cgtcgcagga agatatttac cggaccatca tcagggcgtt
    27061 tattctgatt ctgatcggat cgcttgcgat tgtgtcattg tttctggtcc agacataccg
    27121 gttgacacgt gcgtcactgt cgcgcgtaag ggttttcgca gaagcgctgg tgagaaattt
    27181 acccatcggg cttctggccg tggacaaaga caaccggatt atcgcgtcca atgaacacgc
    27241 ccgtgatttg ttcgccggtg atccgtcgaa tgttgagaga caggatgccg gccggatact
    27301 tccgggcccg ctggtccggt tgctgacaaa ggtgacgatg tcgtccggtc tgatcgaaga
    27361 agatgtcgtg ctttcttcag aaacaaaagg agaacagatc tgggaagtgg tggtggccgc
    27421 ttttgccgat cgggatgttc cggaggggaa aattctgctg gcgcgtaatg tgacagccgt
    27481 cagggggctg gagaaggaag tggcgcacag ccgacatctg aacgccatcg gatcgctggc
    27541 tgcgggtgtg gcgcatgaaa tcagaaatcc gctcagttcc atcaagggat tcgcggttta
    27601 tttcaaacag cggtttgccg gaaatcccga tgatgaggaa acggcggata ttatgattgc
    27661 cgaaactgaa cgactcaacc gcgtcatcgg tcaactgatc gaatttgcgc gacccctgac
    27721 gctgaagaca gaaaaaaccg ccctatccgg tttggtgatg caaagtgtca aattgattga
    27781 agttgaagcg cggaaaaacg gcgtgcaggt ggatgcgcgt gtgccggaag atctgccggc
    27841 tgttgagatc gatccggata aaatcaagca ggtgcttttg aatatatttc tgaatgccct
    27901 ggcggccatg ccggacggcg ggcgcttgtc cgtagggatc tcacaaacac aggccgggca
    27961 ggaagtgctc atttccgata cgggcagcgg catcgagaaa aatgatctgc cccggatcta
    28021 cgatccttat tttacctcca aaccggcggg gacaggccta gggctggcgg ttgtgcaaaa
    28081 gattatggaa gcgcacggcg cttttttgaa catcgaaagt caggcgggtg cgggcacgaa
    28141 ggtcacgttg cggtttgccg tgtcgtaaga cacgacgttt taagaagtga agggtgttga
    28201 aatctggaaa ccttcatcag tcattgagtt cgcaaatacg atgcaggaca tggggcggac
    28261 aaactgttta acgtgaatgc tttcatcata actgtccggc aaagcaaagc ccctgatttc
    28321 ctcaaccggc ttgagaaata atatttattt ttcgggaaag agagaagaca taatgaaaac
    28381 caggctcgat attttgatta cggatgatga tcgtgcgcat cgcgtcatgc tgaaaaagct
    28441 tctaaacggc tgggggtata atgtgaccga agcggatgac ggtctggtgg cggtggaaaa
    28501 agtccggaag agctcttttg atttgattct gatggacatc cggatgacga atgtatccgg
    28561 cattgaagcg cttgaacaga tcaaacagat taatgcggac atcccggtca ttatcatgac
    28621 ggcatacgcg tcggtggaaa ccgccgtcca aaccctgaag aaaggcgctt acgattatct
    28681 gaccaaaccg ctggacttcg acgaattgca ggtatccatt gcccgggcga ccgagcacag
    28741 ccgcttgaag aaggaaaatg aatatctgaa agagaaactg ggtgaaaaat tcgaccggcg
    28801 aaacatgatc ggacagagcc cgtcgatgat caaactgctg gaggtggtcg aacaggtggc
    28861 cgccacacag gcaaccgttc tgattaccgg cgaatccgga accggcaagg aagtcatcgc
    28921 caatgcgatt catttcaaca gcgcccgcaa gaacgcgccg tttgttaaga tcaattgcgc
    28981 ggcgcttacc gagacgcttc tggaatcgga actgttcggt catgagaagg gcgcgtttac
    29041 cggagcggat aagcgaaggg aaggcaagtt cgttcaggcc gacggcggca gtatttttct
    29101 cgacgaagtc agcgaaatgt cgcccgccat gcaggttaag ctgctgcgcg tattacagga
    29161 gcgggaactg acgcgcgtgg gcggacacga tgttcttaaa atcgatgtgc gcgtgatcgc
    29221 cgcttccaac aaggacttga aaaaggaaat ccgcgacgga cgttttcgcg aagatttatt
    29281 ctaccgtctc aacgtcgtga cgctggacgt gccccctctc agggagcggt ccgaggatat
    29341 tcctctcatg gcgcacgcct ttctgaaaat gtttgcggaa aacaatgcca aagtcatcaa
    29401 aggctttacg ccgcaggcga tgcagaagct ggcgagttat tcctggccgg gcaatgtgcg
    29461 ggagttgatg aacgcggtgg agagggcggt tgttctttgc aggacggaaa ccatcggtga
    29521 agaagacctg atttttacca tggcggatca ggccctctct cgcgaggtat tgtcggcggg
    29581 gcagaaaatg ccggcagctt ccgggaatcg ttctctggag gaaatagaga agcaaagtat
    29641 actggaggcg ctgcaaagct gtcgcggcaa caaaagtgaa gccgcgcggc gtctgggtat
    29701 tacacgaaaa acactgcgta aaagactgca taaatacgaa gacggtgaat agagcgcaga
    29761 agtttttgaa gaacctcttt tgtttgtcca agtcggtatt caaaaatgat catatctttc
    29821 acggtatact ttttttacat ttgacctttc agatgaaggc ggcatgtcat gatcatcata
    29881 tcctgccatg ctctttccgg taaaagcctg gattgcgtcc ggtccagcgt ttgaaggcgc
    29941 ggtagaagtt tgatatatcg ctgtaaccca gtcttgtggc aatcgtctca aggggacagt
    30001 ccgacgtctt gagccaatcg agggccttct ttttccggat atccgcaacc agcgatttat
    30061 aggatgtctt ttcatccgtc aggcgacgtc tgagggttct gggtgaaaga ttcaggtgcc
    30121 gggaaagttg cgtgaaactc gggacacttt cctccttgaa aagaatttcc tgccggattt
    30181 gatcggtgat cgtctctttt accttcagcc gttgacacat ttgcttgcat tcctgctcgt
    30241 acgttttcct ggctatggga ttggacatgg gcagcggcat ggaaagcaat gtgctgtcga
    30301 aaataatcct gtgttcaggt gcattaaatt tgaccggaca atgaaagaca ccctcataga
    30361 aagaggcata ggatggtttc ggataagcaa accggatttc attgaccgga atgggggcct
    30421 tgaggatatc gctgacaatc cggtagatgg aaacaaattc tcgctcacag acaaacactc
    30481 gcaggtcttt caggtcgatc agttctctca cctttacaaa agccagtttg tctttgacat
    30541 gcagctcata ttgaaaatag gtgagggtaa gggcgatata cttgaaaaac atggtgatgg
    30601 cttcgagtcc ggtgtcgctg aagattgctg cggcgcccag tttgccctgt accccgacat
    30661 ggtactgttg tccgataata aggccgatct tcgggtccgg caccagaaaa agaattttac
    30721 gcatcaccgc gagttcctgc tcgggcgtga caaacatttc aggatcgtcc agatcgcggg
    30781 gatgaagtcc gctgccggtc agcagagtat tgacatggat accgtacttt tccacataac
    30841 gggccagaat cgcaatcccg ttcacggagc ggaaatatgg caatgtcggg ctcgatatca
    30901 gatggatgga atctgctctg gattgttgat tcatggtttt tatcctggcc gtaaatgaca
    30961 atataattgt ctttcccggt cattgtcaag tttattaatt ttggttaaac taccataaac
    31021 acgtcacaca tcagggtcga ataaaaacat tcatcggggg aggggagcac catgagattt
    31081 tcagcgctgt tgttcatttt gtatcaaatc ctcaaatggg cggcgaaaat gaaaccggtc
    31141 tatcgcagtt acgtcggctc tattcatcag gtcagaatca tgatcaagac ggcggacggt
    31201 aaacggggcc gattatttat ctttgaccgg gggacagtaa gttccaaagg cggcgccgat
    31261 catccgtatg atgcggccat tgtctggtcg gatgcggcca cggggttcag ggtgatggca
    31321 tccaagagta atgaagaaca gttcaaagct gccgccgcgg gcaagatgaa gctcgacggc
    31381 atggcttttt tcgcgcagtg gttcaatgac ggtgtgaaac tggtgatgtg aattttactc
    31441 ctctaacgcc ttccctcaag ggaggagaaa tatgatttgt ccaaaaaaat gagattaaat
    31501 tttttaatta tcgtcagtaa ctgattatta aaggagaaaa acaatggcaa aaataatggg
    31561 cggacatctg gtaggaaaat atctgaagga agtggaaaat atcgatatcg tattcggaat
    31621 ttccggcgga catatcgaag cggtgctgga cagtttcacg gagtacaaga ttcgcaccat
    31681 cgatgttcgc catgaacagg ccgccgccat gatggcgcat gcctggtcgg tttacaccgg
    31741 aaagcccggc gtatgttttc tcactgcggg accgggattt gccaacggcc tgaccggcat
    31801 tgccaatgct tatctggata acgcgccgct ggtggtttta tgcggcaggc atccgctgcg
    31861 cgacgatttg aaaggggccc tgcaggaaat caatcagatg gacatcgtgc atcccatcac
    31921 caaatgggcg gccacctgtt atgatcccaa acggattccc gaatatcttt ccattgcctt
    31981 ccgctacgcg gtggagggac gccccggtcc ggtatttctg gagctgccgc cggatattct
    32041 gggtgttaag atcgaagaat cgctggcgcc gatgccccgg cgtaccaccc gcaaatacac
    32101 ggtgcatccg gacgacgccg atttgaaagc cgctgcacag atcatcaaca atgcaaaaaa
    32161 tcctctgttt atcggcggca gcggcgtgcg cttcagcgga aaatccgacg cgctcaaagc
    32221 gtttatcgaa aaaacgggaa ttccctttat gctgatgaat tacggacgcg gagaattgcc
    32281 ggacagtcac tcccattcgg tgatggacat gggcagcgtc ggtctgatgt tcggccttcc
    32341 ccagacggat gtgctgattg ccgcgggact gaggttcaac tggcttttga tgtcgggcag
    32401 cctcattccg gccgcgacaa aagtagtgcg aatcgatatc gatccgcacg aaatcgaccg
    32461 taaccgcatg gcggacgcgg ggctggtggg cgatgtcgga tcggtgctcg aacagctgac
    32521 gccgctggcg gataaaaaag atcacagcgc gtggtccgaa acgctgaaag ccgccggccg
    32581 ctcgcttctg gattacgaac tccggatccg ggagacggcg acagtcccga ttcatccggt
    32641 gcgtctggtg gcgcagattc agaaagtcgt gggcgacgac acagtctttg ttacggacgg
    32701 aggcgatacg aattatttcg gccttgcttt cgagacccgg cagaaggcgg gcgtcatcgc
    32761 cgccgccgcg gggcttttcg gatgtctggg aacgggcatt cccttcggca tggcggccaa
    32821 actggcgcgg ccggataaaa aagtggtggt gctcaacggc gacggttcgt tcggtttcaa
    32881 cgccatggaa ttcgacacga tggtgcgcca taacatcccc gtagtggtgg tcatcaacaa
    32941 cgactgcgcc tggggtatga tcaagcacag ccaggaactc tccatcggcg ccgaccgcct
    33001 tcagtgcgcg gagctgggcg tccggcatta cgagaaagta gtggaaggcc tcggcggaca
    33061 cggcgaattt gtcacgaaag acgaagaaat cattccggcg ctggagcgtg cgtttgcctc
    33121 cggcaagccg gcgtgcgtga acgtgatgac ggacccgacg gtgacgagtc cggcaacacc
    33181 gttgttttat caggcgctca aaatggagta gcaacatgaa agctttgttg ctgaatcttg
    33241 atcctttgcg gtttgtggcg ctgcatgcgc ttcgccccct gagcaaaaaa ttctgctatc
    33301 agggaccgtt ttcgacggtg aagctggttg atattccgga accggttctt ccctctccgg
    33361 aatgggtgaa gatcaaaacc cggctctgcg gcgtgtgcgg aagcgatatc aatctcatgt
    33421 tcatgaaaga ctctccgtcg gcaatgccgt ttacctcgtt tccttgcgtg ccgggccatg
    33481 aattctgtgg agatgtcgtt gatgtcggcg cggatgtgaa aaaaataaaa aagggagatc
    33541 tgatcacggc cattcccgcg ctgaactgcg aaacacgcgg aatcaaaccg gtgtgcaaaa
    33601 gctgcgcatc cgggacaacg gccaattgcg ataattttgc cgagggcgcg ttcgcgcccg
    33661 gtatgtttac cggtatttgt aaagacatca acggcggatt tgccgaatat gttattgtgc
    33721 atcaaagtca ggcccgtgtt gtgcctgcgg gcgtgtcgcc ggaatcggca gcgcttacgg
    33781 aaccgctggc ggtggggttg caggctgtaa tggacaacag gccggcagac aaagacaagg
    33841 tgctggttat cggcggcggg gtcattggcg cgatggtggt caaaagcatc cgggcgctgg
    33901 actgcacctg cgacattacg gttgtcgagc cgtcaacgtt tgcggcggat tatataaagc
    33961 aatgcggcgc caatcggacg atgaaaggcg ggattattga ggccgctgtg aaaattacga
    34021 aaggcagagc ctacagaccc atgctgggtg aacgcatggt aatgggcggc tttgacaagg
    34081 ttttcgatac ggtgggcaat gcgcgcacac tgaatctgtc gcttcgcgtc acggcaacca
    34141 acggcacgct ttccgttatc ggcatcggcc aggaggtcaa actggatctc acgacattgt
    34201 ggctcaaact gcagaccgtc aaaggctgtt acggttatcg ctacaattct gtcgcttcaa
    34261 gcaggaaaca cacgttcgac atcgcccttg agctgatcgc cagccggaaa attcaggtgg
    34321 atgacatgct cacgcaccaa ttgccgttgg aaaactaccg ggaaatgatt gagatcaatc
    34381 tccaaaaaga aacccgcaag gccatgaaaa ccgctttctg ttttatgtaa aaagccgtaa
    34441 gcaggttatt gcccggcgcc gcgaggcaag gagtgtcctg aaaagcacat gtaatgaagg
    34501 cttgaaaatt ttgacctgtc tcattgaata aaggggagtg agacatgata tattttgacg
    34561 atttgttaca agacgataat atttgtgacg ccgtatcccg gattgccggg gagtttcgtg
    34621 aaaaacacaa ttttccggaa gttcgtcagt tgggagtggt tgtcccggat gtcgaaaccg
    34681 ccgcgacgca actggagaaa cggggcatcg ggcctttctt tattgcttcg gatacactca
    34741 aattctggaa cgaacgcggg gaagacagaa cgttcagcgg caaactcggc atggccatat
    34801 ataaaggata tgaggtcgaa cttctggagc cgggtatcgg atcggccttc tataaatcat
    34861 gtgtggatgg aaaaggccgg atggtcctgc agcatctggg ctttctggta aatgatgtgg
    34921 atgattacag aaacaaattg gagaaggccg gttgtccgac atggatcaga gggcagatta
    34981 aatcctttcc cgtggtcatc gattttgtct atatggattc ggttgcgcag gcgggcatta
    35041 tcctggaatt cattgatatg cggtttctgg gtatcggcgt caagcctccc ggaataatct
    35101 accacggcct cggaaaactg gaaaaaatca ccggcataag atgcctgaaa ttctgaaaga
    35161 acccatgacg gcatcccgga ttgtttgtcc ggcgataaaa aagacgacgg taatcaaagc
    35221 gccggttaaa ttctatccca tgaacgttag ccgcatgcta tccggcggaa aatttaaaat
    35281 aatatggatg aaaaggaggt cgttatgttg aaaatatcca aaatatgttt tgccgtatcg
    35341 ggacttcttc tgatcgtgga ttcaacctta atgattttaa acaaacccaa tcccctcggg
    35401 cttccgcttc cgtgtccggt gaccctgacg attctgggtg tgggattgat tttgttttcc
    35461 attgctaaaa taaaataatc gcttcctctg ttcgaccatg tattgaaaaa taaccgtatt
    35521 ggttgcgatc tgtctggctt ggaccaaaat gcgccgtcga aatagtatgc ttaatgtttc
    35581 ctgcagcaga cggtttccgg ataggaacat cgcgatttga gatcgttggg ataatttttt
    35641 ttaacgttcc gtcagctgat aatctttctg ccggaataat ctgaggcagg cgtctacaac
    35701 gccggcatca taaagaacgc ctttgttttt ttcaatttcc gcaagtgccg catcaatacc
    35761 cagagaggcg cgatagggac gatgcgaggc aatggcttcg acaacgtccg ccacggacaa
    35821 aatgcgtgat tccagcagaa tttcatcgcc tttcaggtgg ttgggataac cggacccgtt
    35881 gattctttca tggtgttcac aaacgatttg tgcaacgggc cagggaaatt cgatgtcttg
    35941 taaaatgtca tatccggatt gagaatgtgt tttgataaga tcgaattcca gattggtcag
    36001 tttgctcggc ttgctcagaa tttcagccgg aatggaaact tttcctatgt catggatggt
    36061 agctgccaca cgcaatcctt caatccgatc attcggtaac cccatttctg tggcgatcgc
    36121 gcgggcaaga tcactcacct ggcgctgatg gcccgccgta tagggatctc ttgtttccac
    36181 cagggtggct atggctttga ccgttcctcc gagagctctt ctcaggcgtt caacgttttc
    36241 tttgcgctct gtgatatcct cgataatccc ctcgtaatag ataatctgtc ctttttcatt
    36301 gcgaacagcc tgcattgtct gggacaccca gatggggcgg tcgtcttttc tgcgaaactg
    36361 gatctcgtta tttttaatgg agcctttctg ctccattacc tggataagtt ttgtgcgttc
    36421 ctgaggttcc aggtagattt gagaggccat atcggttatc tcggttataa gatcttcctg
    36481 ggagtcgtat ccgaggattc ttgccatgga gcgattggcc aaaatgaatc gtccttcggg
    36541 tgtcgctcga taaatgcctt cctgtgcatt ttcaaagatg tttcgatatt gttcctctgc
    36601 ctgcttgcgt tcgctaatgt catgggcaag cgtcagaagg tagcgcgtct gttcgatttc
    36661 tatgaaccgg gcggaaaaaa gcacagtccg gatctgaccg ctcttctgtc tcatcataat
    36721 ttctacgctg ctggcttctc cggtttgcgt cacaatgttg acgattttct cgcggtcttt
    36781 caaattgacc cataaatgaa ggtcgtgagc ggaaaaaccg atgatttctt cacgggagta
    36841 tcccgtccag ttgacgaagg cttgattgat atcgacaaag gttccggtgt cgacatcggt
    36901 aatggccaga gggttggggt tgagatggaa aatggaagaa aatttttttt cggatttgag
    36961 gagcttttcc tccgctttcc ttcgttcggt gatatctctg aaaatgccga taagaaattt
    37021 ttttttatcg aacgtcatga gtgatgcgct gacatccgag aagaagacgt cctgatcttt
    37081 cttcaacagt ggaatgtttt gagcaacgga aatttctcct gccgacaatt tgtcaaattg
    37141 ttcattgacc aaggcaaggg atgcttccgg atgaatgtcg ggaaggccca gtttctgcaa
    37201 ttcttctttc gtatagccca gcatgtcaca aagtttttta ttggcggcaa aaaaggtttt
    37261 ttcacggaca tcggcgataa gaattccgtc ggacgccgaa tcgaaaatgg ttctgaatcg
    37321 caattcggct tccttaagca aatcgactcg ttgcttcaac tgagtgttga ggtcctggat
    37381 atttctgaaa ctgagttcat ttaaaaagga ataaaagaca aaagcccacg acatcgggag
    37441 cagggcgccg atgtagtctt caattctgtc aaaccgggaa gaaattccgc tccattcaac
    37501 cgccagacaa atcccgtaca gcagcagaaa aaaagaaagg catgccagta acacccgggt
    37561 gctgatcaga aaataatcat tggctcgtct ggccaatccg gccatggccg ccaatgccgc
    37621 caggatcaca ataatatcca gaaatgccac cgggtggatc atgtgtttcc ccttgttctg
    37681 cctgggatca tccagcacca aacggcgaaa agtacagcgc tggaggcata gcataagtgc
    37741 tcgataaaat tgagagatgc ttcaaagaaa aatccttcca ggacggtcat catccagcct
    37801 gtcgtactta aacagtaact gacaaagagc agttgaaaga aaggcgtttg tttgagttgg
    37861 cgataattca ggacaaggaa aaccagaagc gtcaagccga taactatcat gatcatttca
    37921 ttttcatgga gcatctttga ttcccttttt cagaaatgct tatgcttttt ggatagttat
    37981 cgtattgaag attctattac atcttttttg aggcccctga atttattatg acgttgttgc
    38041 ccggttcgat accgggcatg ggaacacgtg gataagaatg atcatcccat gatatgaatt
    38101 ataaaaaaca ccatccgttg tgtcaagagg attatgaccg agaagggcgc tgtgaaaacc
    38161 ggtatgacta agaatcatga tgaagtttac atatcggcaa gccatcggga agcatgaaga
    38221 cggcaaaaaa agacggtaag attttttagc attgtgtctt ttggtttcaa ggtgatttct
    38281 gttataatcc gtaaaaagta tctctgcgca atgatcaaca ggaaaggaga atggcatgtt
    38341 tgattatatg atgaccaagg aacagttaaa gatcagggac gaggtgcggg actttatcaa
    38401 gtgggttccg cgccagatgg ttctggatat ggatacggac aagattaaat tccccaaaga
    38461 gtttcttcag gaagccgcac ggcgcaatct catggggtgc cgttatccga aaaaatgggg
    38521 cggccgggat atggactggg taaccacctg catggttatg gaagaggtgg ggacgatcgg
    38581 ctatgaaatg gcctgtgtct tcggcgtggg cgcggaannn nnnnnnnnnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38701 nnnnnnnnnn nnnnnnntac ggcctgatgg ggtgccgcgg cggcggaacc gggcggatca
    38761 tctttcgcaa tgtaaaagtg cccaagaaaa acattgtggg gaaattacag ggcgcgtatg
    38821 ctgttttcaa tacaatgatg attccggaaa ggctcggcac ggccgccatg accatcggcg
    38881 ccgcccgtcc cgctctggaa attgcaacca attatacgtc acgacgcaag gcgttcggcc
    38941 agattattgc gcagttcgag ggcgtcagct ttcagattgc cgaagcgacc atgcttctgg
    39001 atgctgcgcg ggcgatggcg tatgtcaccg cgaaggcggt ggatgcgggt gcggatatgc
    39061 acagaatccg gcgcatggtt tcacaaagca agaaatttat caccgaatcc tgtcagaaag
    39121 cggttcataa ctccatgcag gtcatggggg gaatcgggta caccaacgtg tttcccatcg
    39181 aacgcattta ccgcgatata cggctggcgt cgatctggac gggcaccagt gaggtgatgt
    39241 ccatgatcag cgcgcatgag tggtaccggg aatatctgat gcagaaggcc ggacagaaaa
    39301 ctcgcgagca tgaactggac gccgaagaag cccatgaaga agaaaagata tacgatgatg
    39361 aggatatgtg gaaaaaggga tggtaaaacg tccgccgccg gatgacgaaa tcctgttttg
    39421 ttgatcgctc ctgtatcagg tatttttcgc cggttgacgg cccatgatcc cgttttcctg
    39481 aagattgatc gctgaataca tcggatcgcc ctgcttgaaa accaggcagg gcgatcttaa
    39541 cgcgcggata tcttccaaag ggtttttgtc cagaaccacc atgtccgcgt atttcccggt
    39601 ttccagcgag ccgattttat cttccagacg aagaattttc gcattgctga tggtggcgca
    39661 gcggaggatt tcgatattgg taaacccgtt gcgctgcaga atttcatatt cccggtaatt
    39721 gccgccgaaa tagcagaaag gcattcccgc atcaatgccg cagccgatga gaattcccgc
    39781 ctgattcatt ttcttcagat tggcgtagcc gttcatcatc atgccgaaat agacgtcggg
    39841 atcgacgagg aatttcttgt gctcccagag gttgtccctg ccgatcgttt tataatattt
    39901 caaggctgcg agattctgtt tgtgcagcac cggatcgcag tgctggaatg cttcatggcc
    39961 aaaataattt tttctcacat gtaattcgtc catgacgtcc ggtgttgcat acggtggcgg
    40021 aatgttgtcg aaagcttctt ccatgagata ggattgggca accgtcatgg tgggaacaat
    40081 ggccacatta ttttttgcca ttctcaatac gtcgtcatcc gaaagaatcc tgtcggcaat
    40141 cagatgttcg atggagtgaa aaggataggc catcgcgcga tcgaagccga atttgaaatg
    40201 atggtgtccg accacgggaa ggccgttttt ccccgcaaag ttaaagataa tatcgagttg
    40261 ctccttgctg tacacgggga tggccttgtt ctttttacaa aaaattgtcc gattatccag
    40321 cgtcagtttg atgaatgaag cgccccgggc attttcttca agacattctt ccatttcttt
    40381 ggtgtttttg aagttattca tgatcatgcc gatgaagagc gaagccggca gggcgaaaaa
    40441 attaatgtcc gaaggcggaa tttcaggatg cgatcccatg acgttcagaa tggaattgca
    40501 gtacacaacg cggggaccgg gcatgtttcc tttttcaata tcccggatga acctcttcag
    40561 cagaaccggg aaagcgccca tgtcccggat ggtcgtgatg cccgattcga cggcggaaac
    40621 gaaattctgc ttttgctggc gggcatggtt cggaaggtcc gtcatgcgca ttgaaaagac
    40681 gggagaggct gtggcatggc aatgcgcgtc gatcagcccc gggatgagaa acctgttttg
    40741 aagatcaaaa accatcccgc gataattatt tttgatgttt ccggttccca tcgcggcgat
    40801 ggttccattt tccacaagga gccacgagtt ttcaacggtc agtcctgacg tcacatccac
    40861 caggttggcg tttttgatga gaaaagttgc atcatcaatg gccggcacat tttttttggg
    40921 tatggcgcaa ccatgcagag agggcagggt gagggcctgt gccgccagca gggcatattt
    40981 gagaaaatcc ctgcgcccaa atgatagaag cgattcgtta tccatgcgtg accgttgtga
    41041 caaccaataa tattgaagca attataatcg gcgggttttt cgtgtcaaga tatattaaaa
    41101 ggttcttttc tccggcagga agttgcatac actatctttg gttgacaggc tgcatggttc
    41161 ttcttatact ttcaaaaaaa tgaccctatc gtgttttgtg caatcgcctg tcggaaagat
    41221 cattgtataa aaagtaagga gtctgatgcg acaatacaaa tgcagcgttg aggttttgcc
    41281 ggatgtcttc tgcatcagac ttcctctgcc cggtcgtaag ccgggacccg tcaatgtcta
    41341 tcttttcaag ggacgtaaaa ataccctgat cgatacgggc atggctcaaa cggcaggcat
    41401 tctgaaaaaa gcgctggcgg agcatcacct tggttttgcc gatattgata ccatcatcat
    41461 tacacacgga catccggaac atttcggggc tgcaagaaaa attgtccttg ccggccgagc
    41521 aatcgttatg gcgcatgagg gagacagaac gttcatcgaa aaaggatacg gtatatccgc
    41581 aaagcagtat aaaaaatttt tccgcatcac gggtgttcca cgcaacctgg aaattatttt
    41641 gaggctgctg tttttctttt tcaaacggat ggcagatccc tgtcgggttg ataccattgt
    41701 gcgcgaagac gatcaaatcg acatcggccg ataccgggca aaaattctgg aaacgcccgg
    41761 tcattccagg ggatccgtca gtatttatct tgaacaggaa caaacgcttt tctgcggcga
    41821 tacggtgatc aagcacatca cgccgaatgc ttttgtcgtt ccggatgaag acgcaacgct
    41881 gccggtccgt ttgagccagg atgaattttt caaatcgctt atgaagatca aaagcctttc
    41941 accgcggatc gtttattccg ctcacgggaa gcccattgac gatgtggata aaatcatcgc
    42001 cggatatgaa aaagcttttg ctgaacgcca ggaaaatgtt ttggccttaa ttcatgcagg
    42061 agaaaaaaat atttaccgca ttgcgagacg tcttttcccg aacatcggcg ggtggcggct
    42121 accgctggaa ataattcttt ctatttcaga ggtttatagc gccattcaga ttcttcaaag
    42181 aaaaggaaaa gtctgtctga gaatgagaaa caatcttttg gaagtggatc aataccgcca
    42241 tccataatga gccggaaaaa tttaataaat attatattga cataaaatat tgagtttagt
    42301 ataaattacc caaataattg atatgcaata gaatattaaa agagatcatt taaaaaaatt
    42361 attataattg cagtgcgaaa ggagaacatt atgaagaaaa ttggattttt agtagttatg
    42421 ctgatgttga tggctgtctt cacgacggct caggcggcag aagtcagagc cggtgggtta
    42481 cccgctttgg ctgcagacga tagtaatgca accagcaccg tgaatctgtc acgggaagga
    42541 gaagtcagag ccggtggttt ccccaagatg gataaatatg atacatgcaa cgattgtact
    42601 cctcctcagt gtccgcctcc tcctccccct cctcctccgg tagaagagaa agtatgtcct
    42661 ccgtgcccgg aatgccctgc atgtcctcct cctccggtcg ttgaagcgcc caaacctgca
    42721 attatcgaaa aaggcagaca gaccctggat gtgaagtttg cttttgacaa agccacgatc
    42781 aaacccggtt actataagga cattgatgcc ttggctaatg tcatgaaaga ttatccggac
    42841 ctcaacgttg tgattgaagg ccataccgac agtgtcggca gcgacgcata caacaagaaa
    42901 ctttctcagg aaagagctga tgccgtcaaa aaatacatgg tcgacaacgc cggaattaat
    42961 gccaatcgcc tcaaagcgat gggatacggc gaggaaaaac cgattgcttc caacagcacc
    43021 gcggaaggcc gtgcacagaa ccgtcgtgtt gaagcagcgg ttgactatgt gatcgagaaa
    43081 tagaaaagca gttttccatg cattggatca aaagccctgc agtttcctgc agggcttttt
    43141 tatgtgttcg gtgggggagt gcaaaatatc cgttgacgcg ctctgaagag catgaaagaa
    43201 aatccaagcg cggagaagat gaaatgagct gttgcgggcg cctggttttt ctgcgggcag
    43261 acggaagatt atccggaaag gatcacgctt agcttttcaa ttcccgcgta taaaaaagaa
    43321 caggcaaaca ccacaagaaa aacagcgcac aaagacagaa tgccccgata caggcggtcg
    43381 tttaaaaaat gtctgccgcg ggtgactgcg gcagagacaa aagaatacca gctcaaatcc
    43441 gccagaatat gtccggcaaa gaaaaaaccg attccccgta aaccaaattg ccgggaatac
    43501 agaatgtatc ccagcccgat agtcgcccac cagataatcc agtaaggatt ggccacgctc
    43561 atcaggatgc cgtttataac gggatgattc ccggattttt catgagcatc ccatgcaagg
    43621 cgcacgttag gcagagagcg gaacatgtta aacgccatcc ataaaaggat aaccgagccg
    43681 gccagcgccg tgacgacgaa aacagcgggc atctggaaga acggcgccag tcccagcagc
    43741 agggcggcca gcagcagtat ttccagaagg gcatggccgg caataagcag ggggccggtc
    43801 atgaacccgt gccgggagct ttcgctgatg gtcaccgtta aaagcggtcc cggcatcatc
    43861 gcgccggata aggcgataat aaaggatgaa acaaagatgg tgagaagggc agtcaacata
    43921 cgctttgcat ggttttattt tttaccgatt tttttggcga tctgctttgc cctgttaaat
    43981 tgcgacgtat ggataacggc atctctgacg ggacttgcgg cagccagttc gcgcatttga
    44041 cggactctct cttcgcttgg aggatgcgtg ctcagagcat cgttaagatt ttttgcgatg
    44101 gcatttccgc tttggccgga ttttttttct atctccagaa gtttttcata aaatgttccc
    44161 acctgatctt tgtcatatcc ggctgctacg gcatatttga aaccgatacg gtcggcctcc
    44221 atttcatctt ctcgtgaatt gaccagaaac gtgtatttct gcgcaagcag tccgccgccg
    44281 gcccccagag cgccgccgat cagggcggct cgcgccttgc aggcagaatc gccatcgctg
    44341 caaagtacgg tgcccagtcc gtaaccgaca atggcgccca cgacggcgcc gccggtcgca
    44401 tacatccagg atttattctg ggcctgttcc atgacgtaca ttctctcggc ggtatgccgg
    44461 gcaacgacat gaccgatttc atgcgagatc acgccggcaa gttccgcttc attcgaggca
    44521 agggcaatca gaggaacggt gacgaagatc gttcccgcgg gcagtgcaaa agcgttgata
    44581 tcggccacat caacgacgct gaacgtgtaa tgatagggat tgccttccag atggttggca
    44641 cggacaattt tcattcccag atccgagata tatttttgga gttcctgatt cttggctgac
    44701 ggataatctt tcaaaatctg gggaagcgct tcccgtgtca ggcgttgttc atcttgtatg
    44761 gttaaatttg tttgttgatt gaaattggag ccttcacgat agcgcgaggc atgggtcggt
    44821 gcgcaggacg aaaccacaat gaccaacagg acggccaaaa atattttttt atacatttta
    44881 tatctccccg ggcggtaatg atattctctg atcatccgct ctgatcttag ataaccatgc
    44941 cggatacgtt ctgtcaaggt tgcgatcggg cggatataat gcattgacca ggagaaaaat
    45001 ttgttttaaa tgttttatac ttctgatgaa atgacgaaaa ttatggagac acggacatgc
    45061 ctttttttaa gcgcggcaat gcaacaattt attttgaaga tgatggatgg ggagacccga
    45121 taatcgccgt tcacggactc atagaaaaca ccacctactg gaaaacgacg gcggaggaga
    45181 ttatcaaaaa atatcgtttt atccctatgg atatgcgggg tcacgggaag acggtcgttg
    45241 aaggagaatt ctgcggtttt gatgcggaaa cgatcggaga cgacattgag gcgctggccg
    45301 attatctgaa tatcggggaa tttcatctcc tgacgcattc cacgggtggt tttgccggcg
    45361 tgcgttatgc catgcgcgat tccggcaggc tggccagtct gattcttacg gatacggggt
    45421 cctcgacgtc cgtgataccc ggtgatgtcg aaacgatcag aaagttcaat gataaactgg
    45481 cctgttattt tgaaaagttc aactgggagc agattgtcgc gaatctgtgg gcgaatccgt
    45541 ttcctttttt tcgagggatt gtcgaatcac cgcaggcccg cgatcttatg cggtcagctc
    45601 aggacatgat cagtctgggt gaccggtccg acatcgcccg gttcgtcaga tcattttaca
    45661 cggatcctga tccgaagata gaagggttgc gcggaattcg ttgcccgacc ctgatcattt
    45721 acggtgaaaa ggatgatcta tttatagaga ccagcaaaat gatggcccgg gagattcccc
    45781 atgcgcaact gctggagtat cccggagtcg gacatatgac ggcgctggaa tgtcctcaac
    45841 gtctggcaaa ggacattatc gaattcatca aagaaaacag ccgacaaaca tttgatttgt
    45901 aatctgtctt gacaagagca tttttttgcc gtcgatacca tctgccggcc aatccgggat
    45961 taacggaagg gcaactgaag cctcaatttt taaggttata gcaaaaaaat gattgacaaa
    46021 gaatctgtga tgtgttattg gtcgaaccac ccggccaata aggccgaaag ctaggagaga
    46081 caaccataat gacccgaaag gtttttgccc gtcctgttac gcgttcacgt cttcacgaag
    46141 aaattgtcac cgtcatccag cagcagatca tgtccggtca gatcgcgccg ggagaaaagt
    46201 tgcccacgga aagagagatg gcggaaaatt tcaatgtcaa tcgggccacc gtgcgcgaag
    46261 cattgagaaa actggagaat atcgatcttc tggaaatccg tcatggcgac gggctctatg
    46321 tcaaagatta tctcaacagc ggtaatttcg atttaatcaa ggccgctgta aagatggacg
    46381 gagggacgga aattatcttc aatattctgg aggcgcgccg ttatctggtt ccgcagatgg
    46441 cttatctggc ggcgcaaagg cgcacctcat cggacgtcga cgatctgaag cagctccttt
    46501 ctgaaaaaaa tataaccatg ctggaaaaag atatccggat acatcagcag atcgcccgct
    46561 gcacgcataa tctgctgtgt accatcgggc tcaatttttt caatcagatc ttccgcgatt
    46621 acggacatct ttattttaac gatccggaaa atgtcgaacg ttcccggcaa tttcacgatg
    46681 aaatatttga agccatcagg aggaaaaagg ctgatgacgc ccgcaacatc atgagagacg
    46741 ttcttattta cgcggaggaa gcggtgaaaa ataaaattaa aagaccaacg tttgataagg
    46801 agtgatggtc atgaaaagat ttattgaaaa ttacacaggt gagaaatttg acgttgctgt
    46861 aatcggcgga ggcatcagcg gtgcgtcggt cgcttatgaa gcagccacgc gggggctgaa
    46921 agtcgctctc ctggaaaaaa atgatttttc ctgggcgaca tcggcggtga catccaaaat
    46981 gattcacggc ggtttgcgat atctggtaaa cggtgaagtc ggactggtcc gcgagtcgct
    47041 gcgcgaacgg cgtgttcttg agaatattgc tccgaatttt gtctatccgt ggccgatgat
    47101 gatgacgcat tacaaaaagc cgctgaaaaa taataagtgg gtggtgaaag tgggtatgct
    47161 cgtctatgac gttctgtcct acgacaaaaa tttcacatgg gatccctgca aaaaaattcc
    47221 cgtccacaga acgatttcgg cccggcaagt tctccaggat gagcctcacg tccgcgctga
    47281 aggattgacc ggcgcttcta ttttttacga ctgtaccagt cttttcccgg aaaggctgac
    47341 gttggccttt atcaaatccg ccgtcgccca cggtgccagg gcagccaatt acgcgagagt
    47401 ggaaggtttt ctcatggacg gcgcaaagca tgttctcggt gtaaaggtga aagatttgtt
    47461 cagcggtaag atgcacaatg tgcccgcaac cgtaacgatc aactgcggcg gaccctgggc
    47521 ggatatcgtc ctgggcatgg ccaaaccgga tggtcaggtc aacgcaacgc ttcggaggtc
    47581 cgaagggatt catatcatca cgtctaagaa actgctgacc ggcaactata caatcgggtc
    47641 gatgacgccg gagggacgtc atttcttttt gattccgtgg cgcaatcacg cactgatcgg
    47701 cacgacggac aaaccgttta acggtaatcc ggacgactac cgcgtcacca aagaaagcat
    47761 catggaactg attgctgacg tgaacagctc cttcggcgac ggcaaactga gctatgcgga
    47821 tgtcaaacat acctacggcg ggctgcgccc tcttattgaa aaagaaacca gcgaaaccta
    47881 ttcttcttcc cgcaaatatg aaatctacga taataaagat gacggattgg acggactgat
    47941 taccgtggaa ggcggtaaat acaccaccag ccgcaaactg gccgaaaact gtctggcgat
    48001 cgtcgccaaa aaaatgggaa gaaatcttgg ccggagcgtt acggacaaac aatatctgac
    48061 gggctgcgat atcaaagatc tgtgtctttt tattgaggaa gcgaagcgtc cggctgatgg
    48121 attgagcgga gccaccctcg aatatctggc gcgcaattac ggtacggcgt accgggacgt
    48181 cgtcgggctt gcgcgtgaga aaaaaagtct gtccgagaca ttaaacgagg acggcgaaat
    48241 tatggcgcag gttgtgtatg ccgttcgtga tgaaatggcc caaaccctgg ccgatattgt
    48301 catgcgccgt acaggtatcg gcacactggg caatcccggt gacgatgtgt tgcgtaaagt
    48361 tgcacaggtt gccgctgatg aattgcactg ggatcagcag agaatggaaa aggaagtgga
    48421 agcggtcgtt aatctgctga agatacctgt tgattaaaaa attgaatgaa catgaaaaga
    48481 gttcaagaga aaaggaggac atggtgatga tatctcatta tttccggcca aactggacgg
    48541 aaaaggcacc ggcagccgga acgtacagat ccatttttaa atacggagat cttcacagtt
    48601 tcaaacatcc cagcgatgcc tggtacagga tgatcaagga agaatttcat ttaaacgaca
    48661 aggacttcgc ttcaagagta aaagaaggac ttgaaccggt ttcgttaaac cgtgcccagg
    48721 cactcaaacc gcaacagatg gattgcctcc ggaatattgt gggcggcgaa aatgttgcac
    48781 tcgatgatta tagccgtgtg aaatacgcat caggcaaaac cacggaagag atgctggaac
    48841 tgcgtttggg gattatccgg gaagtcgcgg acgtggtggt gcatccgcgt gacaaacagg
    48901 atgtgcagaa aattgttcaa tactgtcatc aggaaaacat tcccataacg gtttacagcg
    48961 ccggttcttc cgtaaacttc ggttgtcgtc ccgtcagggg cggcgtttcc ctggtggtca
    49021 gcacccacat gaataaactg ctggaggtca atgagttcaa tcagactgcg cgcgttcagc
    49081 cggggatgtt cggtccggct tatgaagagg gtctcaataa agcgcccgaa ctgtttaaaa
    49141 caaaacatcg ttacacctgc ggacattttc cgcaatcctt cgaatattcc actgtgggcg
    49201 ggtgggttgt cacactgggg tccggacagg cctccaccta ttacggtgat gcctatgata
    49261 ttgttttcag tcaggaatat gtcacacccg ccgggacgtt taaaacgctg gattatccgg
    49321 cgaccgcaac cggtcccaag gtgaatgaca tcatgaaggg ttctgaagga tcattcggta
    49381 ttctggtgga agtcaccatg aaaattttcc gctacatgcc ggagaatcgc cggcgtttca
    49441 gttttatgta tccgacgtgg gaggccgcgg taaatgccag ccgggaaatc atgcagtcgg
    49501 aattcggcaa gccggcaatt taccggattt ccgatccgga ggagaccgac cgggggctga
    49561 aactctacgg catgccggat tttgtggata aaatcctcgt gatgaaggga ttcaagccca
    49621 tgcagcgctg tctgagtctg ggaaatgccg agggcgattg cgattatacg aagcttgtgg
    49681 tgcgcaaaat caaaacggtt gcgaaaaaac acggggcgat atcgctgacc ggttacgcgg
    49741 caaaaaaatg ggagcacacc cggtataccg aaccgtatat gcgcgaagac cttggcgatt
    49801 acgacatcct gatcgatacg ctggaagctg ccgtgacctg ggacaatctc caccgtctgc
    49861 atcagggcgt gcgcgcgtat atcaagaaca ggccgcaaac catgtgcatg acacacgctt
    49921 ctcactttta cccgcagggg accaatctct attttatctt tattgcgcat atgaaagatc
    49981 ccgaagagtt tcggaaattc cagcggggta ttatcgacaa cattcagaaa cacggcggtt
    50041 cgctgagtca ccatcacggc gtgggacgga tgatcggacc gtggatggag aaacatctgg
    50101 gcaaagaaca gttggacgtt ctgcggacgc tcaaaaaaca ttttgatccg cnnnnnnnnn
    50161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgggcaaa gaacagttgg acgttctgcg
    50281 gacgctcaaa aaacattttg atccgcgcaa catcatgaat ccgggcggtc agctcggact
    50341 ggattgatga aagcagcgcg atgattcgcg gaaatcataa aaaaaggaga gatgactatg
    50401 tcgggtgaaa aaaatacaac gcaggatctg gttctttccg tggatgcggg gacccagtcg
    50461 atccgggccg ctctcatcga tgtccgggga aatatcaagg cgatcgtcaa aacgcccatt
    50521 caaccttatt tttccgaaca tcccggctgg gcagaacagc ggcccgacta ctactgggat
    50581 gtgttttgca aaacaaccca tcagttgttg caggaccagg aagttctgaa aaaacatgtc
    50641 aaaggcgtca cgctgacgac gcagcgtgcc acgatggtca atgtcgatca gaacggcaac
    50701 ccgctgaggc cggcgattat ctggctggac cagcgaaagg ccgactccag aaaaatccta
    50761 ccggcgccgc ttcgtccgct tctgaaaaca gtcaacctcc tggatacgct ggagaacgtc
    50821 attcaggaat gcgaggcgaa ctggatctgt cagcaccagc cggacatctg ggaaaaaacg
    50881 cataaatatc tggtgctgtc cggatttttt acgcatcggc tcaccggcga atttgtcgat
    50941 tcgacgggta acaatgtcgg ttatctgccg tttgacaata agacctacca gtgggcgggc
    51001 aagttcgatt ttaagtggcg gatcttcaag gtggaaaaag aaaagcttcc tgacctggtc
    51061 aaaccgtccg aaatgatggg tacgattacg aaaaaagctt ccgcagagac cggcattccc
    51121 gaggggctgc cggtgtttgc ggccggatcg gacaagggct gcgagatcct cggttccggt
    51181 tgtctcacgc cggaaaccgg atgtctgagt ttcggaacca ttgccacgtt tgacgtggcc
    51241 acgcccaaat atgttgagct tcatccgatg atcccgccgt ttcccgcttc cgttccggat
    51301 acttattata ccgaggtgtc gatcttccgg ggattctgga tggtcagctg gttcaaggaa
    51361 gagttcggcc ttcaggagtg catgcttgcc gagaagacag cggaacctcc ggagaaattc
    51421 tttgattccc tgattcagga tgtgccggcg ggatccaagg ggctgatgct ccagccttac
    51481 tggacgcctg gccgggaagt ggacgctttt gcaaagggat caattatcgg ttttggcgat
    51541 gtgcacagcc gcgcctacct ttaccgggct cttctggagg ggctggttta cggattgaga
    51601 gagggcgggg aattgaccca gcggaagacg aaaataccgg ttacccggct gaaagtttcc
    51661 ggaggcggtt cgcaaagcga cgcggccatg cagattacgg cggacatttt cggcatcgcg
    51721 gcggaacgtc ctcacacgtt tgaaacatcc gcgctcggtg cggccataga cgcggcggtc
    51781 gggcttaagc tttattccga ttttgcgtcc gcggttacca acatgacccg gaccggcagg
    51841 gtttttgaac cggataagga taatcacggt ctgtacgaca atctttacag gcgggtgtat
    51901 ctgaagatgt acgaacggct caagccgttg ttcagagaca tcagagatat taccggttat
    51961 ccggcgggaa attaaacaac gggaacaaat agattgcgat tcaccggaag taaaagtata
    52021 atcattcatc aacaggcagt cgttgcatgt ttgatcggct ggtgaatcca acaggcgaat
    52081 ggatgatcat cggctgaatt caggacatca aggagatttg cattatgaaa aaaatattgt
    52141 tttcctttgt gatgattgtt tgtctttttt ggacaaccgt cgtttttgcc gaactgaagg
    52201 aagggctttg ggaaatcacc tcccaggtgg aaatgaaggg catgccgcaa tccatgccgc
    52261 ccacgacgtt tcgtcagtgc atcacgaaag gcaatcccgt cccccagaat aaagacaaaa
    52321 actttgaatg taagatcatc agccaaaagg tcagcggcgg cactgtcagc tatcaggtgg
    52381 aatgtacggg caaggaaggg gtcgttcaga cgtccggaac gacaacctat acggacaagg
    52441 caatgaacgg cgcgtcgacc accagtttta aaatgaaagg acaaccggcc atgacgatgt
    52501 ccaacaagat cagaggcaaa tatataggcc cctgtccgaa ggcacagtaa gaaacgtttg
    52561 tcgttctgca atcgaaaggt gaaacatgtc attttttata gttctgttcg aagacggttc
    52621 gctcaaaatc tacaaaagca tgcaggaccg ggaattcttg ccggaagggg cccggcaatt
    52681 taaatgttca agcaatgtga ccttacagga tctctgcctg tggctggacg gcgggtgtaa
    52741 aaaattaaga accatcaagg aaatctaaac aggcatttgc cgtcgtcttt gcaaaatcaa
    52801 accgaccgtt gcccggtctg acatcatccg gcgaaccggt ggcggggatg ccgcaatgaa
    52861 ttattttcag aacagaccaa caacattctt tcggatgtcc attccggatc atcaccgctt
    52921 gcggcaatct ccggtgagga aataaccttt gccacgatga tgagagtggt tttcccgacc
    52981 gacttgtgat attaaaatca aagtacgttt cagaaaagat tacaaagaca aaatgaaatt
    53041 tattcccgac gcaatcacaa ccgatcaggc caaagatgcc ggcatggctg ccgcgctgct
    53101 ttgtctgata gcaggttttt tttcggatat aacgtttttt tacggttttg ctctgctgct
    53161 gctttttctg aatatcgttt ggccccggat attcaagccg tgggcgatgg tctggctggg
    53221 cattgcgcac atcctcggcg ctgtgatgtc ccggctgatc ttgctcctga tttttttgat
    53281 ttttgtgttg cccgtcggtc ttgtccggcg aggcctcggc aacgatacgc tgcagttgaa
    53341 agaatggaaa aaaaatcaac attcggtttt caaaatacgc gagcacgaat tcagggcgga
    53401 tgacattaaa catccgtact gaagcgggag acaaacatgg attttttcag agatgtatgg
    53461 ggttttttga aagtcagaaa aaaattctgg cttctgccga tcattctgat gctgctttta
    53521 tgcggagcct tgatgatctt cgcgggcgga tcagccgtcg ggcctttcat ttatacactt
    53581 ttttaagagg aagacatgtc gcctgccatt ctagggatat cggcgtatta tcatgacagc
    53641 gccgctgcgc ttttgattga cggccggatc gtggccgccg ctcacgagga acgctttacc
    53701 cgtgaaaaac ataccagcgt ctttcccgtc catgcggcaa catactgcct tcgggagggc
    53761 ggacgtcacc tggcggatct tgacggtgtg gccttttacg aaaaacccta tctgaaactg
    53821 gaaaggcttc tggaaaccta tcatgcattt gccccccggg gactgaaaag ttttatggcc
    53881 gcaatgcccg tctggatgaa agaaaaaatt tttatgcgta aaatgctgca cgacggtatc
    53941 gccggcatcg gtccgggccg ggtcaaactg ctttttccgg aacatcatct ttctcacgcc
    54001 gcgagcgcct tttatccgtc accttttgat aaggcagcca ttctgacgat cgatggcgtc
    54061 ggcgagtggg cgacagccac catcggtctg ggggagggcc ggtccatcaa aattcttcgg
    54121 gaacttgatt ttccccattc ggtcggattg ctttattccg ccttcaccgg ttattgcggg
    54181 tttaaagtca acagcggcga gtataaactc atggggctgg ccccgtacgg tcatgccgac
    54241 tccgggcaaa ccgctttata ccgaagaaaa attctggacc atctggtgga tatccgggag
    54301 gatggatcca ttcttctgaa tatggattac ttcgattttg ccaccggtct gaccatgtgc
    54361 cgggatgaaa aatgggaaaa actgttcgga attccccgcc gacaacagga aagtgagatc
    54421 ggacagccct acatgaatat ggcgctggcg atacaacaga tcactgaaga tgtcgtctcc
    54481 cggatggcac gcaccgcgcg aaaattaacc ggcagtaatt atctggtcat ggccggaggg
    54541 gtggcgctta attgcgtagc caacggcgcg ttgctgcgcc agaaaatatt tgacgatctc
    54601 tggatacagc cgtcggccgg cgatgccggc ggtgcgctgg gtgcggcttt tgccgcttgg
    54661 catgtcggga tggaacagcc gagaaaaagc gtgtcggaac aggacgccat ggcgggcgct
    54721 ttcctgggtc ctgaattcgg atcggacgac atcaagcgca ctgccaaaac ataccaggca
    54781 tcgtttgtac actacgaaga tttcgatgaa ttatgtgcgg ttgtcgcgga acggatcctc
    54841 cgggggctgg tggtcggctg gttccaggga cgcatggaat atggtccgcg ggccttgggc
    54901 aatcgcagta ttctggcgaa tccctgcctg ccggacacac aaaaaaaact gaatctgaaa
    54961 ataaaatacc gggaagaatt caggccgttc gctccggccg tccttgaaga agacgtggcc
    55021 gaatatttcg atctcgaccg accgtcgcct tacatgatgc tggttgccgg gctgaaggaa
    55081 catcgccgca ggccgcaacc ggaaaatgag cggcagatga ccctgtacga aaggctttat
    55141 cacatgcgat ccgatctccc ggcggtgacc cacatcgact attccgccag aatccaaagc
    55201 gtcagcaaaa gaacaaacga acggttctgg aagctgatca aggcgtataa gcaaaaaagc
    55261 ggttggggcc tactcgtcaa cacgagcttc aacgttcgcg gcgaaccgat ggtctgcacg
    55321 ccggaagacg cctaccggtg cttcatgcag acggacatgg attgtctggt tatggggaac
    55381 gatcttttca gtaaagacga tcagccgcca tggtctgaaa aaaatccatg gcttgctgcg
    55441 gggacaccgt atgccaagga ttgagcgttg aagcgccggc ggctacaaca gcaggaaggc
    55501 atagacgctt agtagaagca ttccccacat cgtggtatcc atgcccgcca gcggaatgaa
    55561 aatcagactg acaagcacag accccaaaca tccgccgatc agatcggccg catacaaagg
    55621 tgaaatgatt gcgcgctgat catcaatctc ataaagactg gcataggcaa agatcccgga
    55681 aacaataaaa cctgttgcag tcagcaggag accggtcagc cagagaccgg caggcagact
    55741 gtttctgatt aagagggcgg ttatcgcgca aaggcagcag gatccggcca caagtataat
    55801 gccgcaccgc cgggttccca ggcgatggtc cgcaaaacgc gccatcagtt tatcaaccgc
    55861 caaagcgccc agcgcaaggc ccgccataaa actcatcagc aacaaaccga tatcccgata
    55921 aatgacgcca tatttgagtt gaaaataaag cagcaggatc gtctccagcg cgatgcctgc
    55981 aaaaccggcc gccgccacga gtattttgcg gcgtagtccg ggcaggaaac ggctgaccag
    56041 aaagaggaca ggcaagacca tcaagaaaca gaccggcaga tatcgcaaca aggtcttctc
    56101 catgatcgag gagaaatcaa aaagcgtcgc gcgagggaaa aatttcgaca gccagatgat
    56161 cagcgcgtat tgataacaaa ccggtctgat atcggaattg agcaagtcat ttttttcatg
    56221 caggagattt ttgacggcgg caaatctgtc gttggtaaaa aggtagcgga tgtaatggtc
    56281 tgaaatcagc cgggtctcta attggagatg ctgcaggcgg ccggtcatga tgtccggtgt
    56341 atcgggcagc ggcgcaaacg atgcggtgac gatgtttgtc gtgcccggca gaaaaagcac
    56401 agcgggaaaa accgaacgca gggcatgata gatacttgca ttgcggccga tcagcggtcc
    56461 cgtccagaga ttttcagccg tttgcaattt aaatccgaga aggcccccgg atttaagttt
    56521 tgccgcgcat tgtttaaaga attcctgtgt ataaaaacgg ttggcttgtc cggatgacgg
    56581 ttccggcatg ccgaccagga tgagatcata cctcccgctg tttgtgagat accgcctagg
    56641 atcggcataa atgatgcgca cctcgggtga ctggagcgac tgacggatgg actccggcag
    56701 gtgttccata acaagcttcg tcatgacggg attaatttcc acataatcaa tatggtccgg
    56761 tctgtatttt aaggcttccc cgatgatgcc ttccatcccg ccgcccagaa tcagaatctt
    56821 ccgcggatgg ggatgctgaa gcattaccag atggcagaaa tattcggcat cggttccttc
    56881 cgtttcaaag gacagggcgt cgttttcgaa tacggaaatc tgatcgaaca ttctggtgac
    56941 ggtgattctg ccgtaaggcg agtcgctgct gtccagaaga ttcggatgat tccaacgggt
    57001 cgagatgtca tgcaaaggtg atgcattgaa aatcaggccc gcaaacattg caagcagcac
    57061 gattgccgtc cgccgcaaaa aagctcccag ggactttcca gcacccagaa gaggcgtcgc
    57121 aaacgatacg atcgagcaga tcagcgcggc gaaaagattc tgggtgtcca agataatcaa
    57181 cagcgtggag aataacccgc cgatcaaacc gccggcgctt tccaccgcgt aggcggctgc
    57241 cagcgtccgg ccggagtcta catacaatcc ggcggtctgc tgaaataaaa gtccggataa
    57301 aagtcccacg ggcagaatcg acagggtcgc aacaaggagc tgttgaaaaa aatcgagata
    57361 ggcgcccggt acgccgccga agatcagacg gctggaacgg atgaaggcga cgtccaggaa
    57421 caggatcatc ccgaacagga tgaacaaaac ggccgggcta tgcctggacg gaagagccat
    57481 ccgtcgtccg gcgatcacgc cgacggcggt ccagaacagc cagatgccca aggcgaacag
    57541 atagatcagt tcgacaccgt agaaagagac attcaattcc ctgagcagga taacctgccc
    57601 caggatggaa atcagtccga tggataaaag ataaacggtc atgcgtttat ttcagctgcg
    57661 attcgctgaa cacgatcgcc tgaaaaattg aaaatgttgc gtcctgacgc cagcatcttt
    57721 tttccagtcc ggccttgctg cataaatgat ccagcatgtc atcgcggctc cagctctgtt
    57781 ccagtgcgac ttggggaagg aaaacagcag aacgtccgtc tttgctcaac acaacaccat
    57841 cccggccgac cataatgtcg gaggggcggg ccgcctgttt catcggggtg agcacggata
    57901 tctcgatgtc gacatccggg agttcctcgg cggttaaggg aggaaaccgt ccgtcattaa
    57961 gtgccgcctg cagcgccatc gcgcccacga gcttgaccag cggttcgtcc gttattaaat
    58021 ggccgatgca gccccgcaag gcgccgtttt ttttgagcgt aacaaagacg ccgcgggggt
    58081 gattcaaatg cgctgggaaa tgccgtgcca gaggaaccgt gtcggtcgta aaaaaacggg
    58141 atagggtttc acgggcaaag gccagcaatg cctttttatc ggctgtgccc agatcgaagg
    58201 ggacagccgc ggttttctgc agatccgttt gagaagaaga aatccccttt tgcccggctg
    58261 tcagtacgac cgcgccgtaa ccgaccacct gagcggaatc gccgacggag acatcgccgg
    58321 aattggcata actgatgacg ttcccgcgat cggcgccgag taatcgggcg gcagccagtg
    58381 ccgcgataat cggcgcttcg ccgcaggcac acgtggagag attcggaatg tttttgctca
    58441 tcgtgtcctg tatcgttttt tggaagacgg caggatcgag tgtggcaacc gcatcaagcg
    58501 tccggcggtc aaccttgacg gcattatccc gtgagggata gtgcgacaga tcggagctgg
    58561 cgacaattaa tgcatttttg ttttttaaaa cttttgccag cgcctgaccg aagcggatgc
    58621 tggtgtgcct gtcctgcgtc ccgacaaccg caggaacgat tttagcgttg ggaaaaacaa
    58681 tctggacaaa gggcacgatc acctcgaccg aatgttcaga gacatgcgcg gatttgtcca
    58741 gcatgcaatc gacaggagat tccgtgagca gggacgcaat gacggttttg tccactgccg
    58801 caatgccgag aggcgtgcga aatccttcgc cgggatagag ggaaactccc tgcaggcccg
    58861 ggctggtgtg attggttccc aggatgacca cgacatcgta cgggtgatga ccggcctgtc
    58921 ggaaaccgtc agcgcagatc tggccggaga agatatatcc ggcatggggc acaatgacgg
    58981 ccaccggttt tttgaccctg ggcggcaagg cgtcttgcag atacttttcg atggtcagac
    59041 gcagtttgtc tgcggatgcc ggatagaact tgccggcaaa agccggttcc cggacgccgt
    59101 cagcggcgtc tttgttggcc tgacaggccg acgtcagaaa aaaaacaatc accggcaaaa
    59161 aacaaagcaa acgcagccat ttcatatggt aggttctcct ttcaggtcca aacgccggca
    59221 attctattct tgcagaatgt acacagcccg ttgcggagat tcatcccggt aataaaaaac
    59281 ccctgtcttt taaccacgat ctttccgcat ttcggacagt aggtgttgtt tcccggatga
    59341 tcggggacat tgccgacata gggataatgc aatcctctgt cacgcgccat acgataggct
    59401 cgttcaagcg tggcaacggg cgtggacgga agattgcgca gctggtaatc gggatgaaaa
    59461 cgggaaaaat gcaacggcac atcggggccg acttctccca tcacccagtc gatcaatcct
    59521 ttcagcatct tgtccgagtc attcaatgtg gggacgacca ggtttacaat ctccagatgc
    59581 gcgcggcttt tggcgatctg cctgatgctt cttagaacgg gcttcaattc cgcctggcag
    59641 gccgaacgat agaaatcctc gctgtagcct ttcagatcga ttttaatggc ggccagcgtt
    59701 tcgcacattt cagcgagcgg cgcctcgttc atgaaaccgc aactgatcat gacgcagcgg
    59761 ataccctgtt gccgggcatt gcgcgcgatg tctgtcagat attcggtgaa aaccgtcggt
    59821 tcgttgtagg tgaaggcgat caccggcgag cggcttgtac gcgcggcgga cgtgagcttt
    59881 gccggcggtg taaaagccac cttgaaatcc tccggggatg actgagaaat ttcccagttc
    59941 tgacagaatt tgcatttgag cggacatccg gccgtggcca gcgaaaaagc ggccgaaccc
    60001 ggcagaaaat gatagaacgg ttttttttct atcggatcga tatggacgga gatggggcgg
    60061 ccgtatacga gactgcgcaa ttctcctttg acattcatgc gggtccggca ccggccgcat
    60121 tgaccgttgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcaattctc
    60241 ctttgacatt catgcgggtc cggcaccggc cgcattgacc gttggcaatg acacagctgt
    60301 tggcgcaaag caggcattgg atgagtgtct gatccttcgg cgtcacgctt cccttgattt
    60361 ccaggggcct ccagtagcgg gctttcggtg acgatttaat cagctcgtcc agataagaat
    60421 cgccgaaggc aaggcccgca tgcccttcct gaaacagtct ctgccaggac ggatcggaca
    60481 gtatcatcca tgtgccgccg gaggtcacgg ccagtccgga gagggttttt aaaaaatcgc
    60541 gtctggaaat catggcgcct ctttaaagat aatccagtct gaagatagga gattgacgat
    60601 gaaagattat aggaaaaatg ttcagggaaa gtcaatggca aataggaaag tcggcgaaaa
    60661 gatgaacgga tcggcttcag gtaatcatcc ggcgagctaa actttcgtcc agaaaattgc
    60721 tgaactcctg cttcacatat gttaaaaagc gatcatgccg aataaaatca atattcctcc
    60781 cgacaaacag actggaatga tttatgccct gctggccata gcagttctgg ctgttttttc
    60841 gcagctgatt cgatatgagt ttgtgatcat tgacgatccg gtgtatgtca ttttcaaccg
    60901 gcatatcaca tcaggcatga cggcggacag cttcatctgg gctgtcagca cgaaatatgc
    60961 cgatttatgg catccccttg tctggttttc cttcatgctg gattatcggg ttttcggttt
    61021 gaacgccggc ggttatcatc tgaccaacct tttgctgcac atgatgagtt cgctgatgct
    61081 cttctggctt tttcaccgta tgaccggcgc cctctggaag agcgccttca tcgcggcttt
    61141 tttcgcgttg catcctctgc atgtggaatc cgttgcctgg atttccgaac ggaaagatgt
    61201 tctcagcgct tttttctgga tgctgaccct ctgcctttat gtcgtttata cggaaaaacc
    61261 ggtcatccaa agatattggc cggtcgtgct tggttttgta tgcgcgctga tgagcaagcc
    61321 gatggtcgtt accctgccgg tcgtgctgct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61441 nnnnnnnnnn nccggactcg attcccggtc tgcgacttgc cggatcggta attttcattg
    61501 cggttgtcag cgttgccgtc gtgcggatga gaagacgaca tccctatttg cttgcgggat
    61561 ggctgtggta tgccgtaacc atttttccgg tgatcaagat cgttcagata tccagcgatg
    61621 ccatggcgga ccgctaccat tatctgccgt cgatcggcat tgccctcatg atcgcctggg
    61681 gaatccctct tttctttaaa gaagaaaact atcgaaaaaa aattatgttc ccggcggcgg
    61741 ttctttttct ggcgatgctg gcttttgggt catggcgtca atgcggattc tggcaaaaca
    61801 gtgtcacgct gctggaacac gccgtgaaag ttcaaaaaga cgtttacgtt ttgcacgact
    61861 atctgggtca tgccctttat ttcgaaggaa aaagccgagc agccattgtg gaatttaata
    61921 aggcggtttt tctcaatccc ggttacgcca gagcctatgt caaccggggg aatgtttatg
    61981 cggcaatccg gaattatgac gaagccctga aggattatca gacagccgtt cgtctgcgtc
    62041 ccgacgaggt cgatgcgcat tatatgaagg gaatggtaca ttcaaaaacc ggccggtatc
    62101 aacaggccat tgaggatttc agccaaacca tcgccctgga tcaaaaccat gccagggcct
    62161 ttcatgaccg gggacttgct tactttcatc tcggccggta tgagcaggcg atcagcgatt
    62221 atgacggagc gatcagacga aacccggatt ttactctggc ctaccataac agggcgtatg
    62281 gttatttcag gcagcgaaac gatgttcggg gttgtccgga tgcacacagg gcctgtgcac
    62341 tgggggactg tgatttgtta accgctgttc aacaagaggg gcgttgccgc tgagtgccgg
    62401 caaaaccggt gcgctttcgg ggttatgacc tcttattccc gatcaggatg tttttctgtt
    62461 tttccttttt ctccatgttc ttttcgataa agattttttt gccggtcgaa ggatcgatac
    62521 cggtaaagta catcagggcc gaataggtcg agggaagcgg ggtgaatatc tgtacctgcc
    62581 ggggattcgt ttttagctct cttgtggtga acgctttgag acgtctcata tcctcttccg
    62641 tgcatcccgg atgagcggca atgaaataat aggtgagaaa ctgtttgcgt tttgaaacgg
    62701 cggatgtttt ttcaaaaaga tgtttgaaat tcagcagggc ctgcgcctgc ggcttgccca
    62761 tgagcttcag aaccggccat tcgctatgtt cgggcgcgac ctttaactga ccggaggtgt
    62821 gatgctcgac gagttctttc atgtacgcca ggcagtgtgc cctgtcgttt aaaagcagat
    62881 cgtatcggat gccggaggcc acgaaaactt ttttgacgcc tctgacattt cgcagatttc
    62941 tcaatagatt gatctgccgg ctgtgatccg ccggcaaggc gtcgcatacc ctcggggaca
    63001 gacatcgttt gccgttgcag gcccctttct tgattttttt cgcgcattcg atgccgtaca
    63061 tgttggccgt cgatccgccg acatccgcaa tatagccttt aaaatcggaa cgtccggcca
    63121 gtttttgcgc ctcatccaga atggattttt ctgaacggct gataacggtg cgtccttcat
    63181 gcagaccgat ggagcagaaa ttacattcac cgtagcaacc gcgatgagac gttatggaaa
    63241 acgtaatggt ttctatcgcc ctgacattgc cccgggcgct gtagaaggga tgaacgtcgc
    63301 gcgtgaaatc aagtccataa atatgatcca gttccggcgt tgtcagatgc ggctgcgggg
    63361 gattttgcac aagataacgg tcgccgtgct tttgatacag gcgcttcgct gtcagcggat
    63421 cgccgttgca atagaacctg ttaaacatcc tgataaatgt tttcttgtcc gcaagcacat
    63481 cctcataagc gggcagttcc agtgcgtttt gcggcggggg ggcggacgca agataacaga
    63541 tgccccggat atctgttgtg tcttttccgt cgcgcagagc ctgcgcgagt tcgacgacgg
    63601 ctttttccgc catgccgtag accagcacat cggcacgggc atcgaacata atcgaccggc
    63661 ggatggaatc cgaccagaaa tcgtaatggg cgatgcggcg cagggaggct tcgataccgc
    63721 cgagtaccag cggcttggtc tccttaaaat accggcggat cagattggcg tacaccacaa
    63781 cggcgcggtc gggacgacgg ttgttttttc cgcctgcagt cagatcatcg ttttcccttt
    63841 ttttcttcgt cgccgtgtag ttggcgacca tggaatccat gcagccgccg gtgattcccc
    63901 agaaaagccg gggctcgccc agtcggctga tatcgtccgg cttatgccag tcgggctgag
    63961 cgataatgcc tacacgaaag ccggcatcga ccagaacgcg gccgatgacg gacacgccga
    64021 taaaaggact gtcgatgtag gtgtcaccgg tcaccagaat gatatcgggt ttgtcccatt
    64081 tcaatttttt taattcgtcg cgggttgtgg gaagaaacat aacgaaaaac ggctcacgat
    64141 tgcggcatcg gccgcttaga gaagattgat cagcctcaat cccagaagga cggcggctgc
    64201 caggatgaaa atcgaaacgc gtcccagcca gcggtgcgcc gcccgaatgg ccgatttgtt
    64261 gagggctttg aactggtaat gtcccagagc tatggcgatg aggacaacgg taaacgctgc
    64321 cagcccggtc atctgatgaa catcgttgag atgttttccg ccggtgccgg caacatagaa
    64381 aaatgccatg atgattccca cagtgattcc cgccaggctc aaagtgttga gggttttatg
    64441 aattttcagc cagtttttct ttctgcggaa aaacatggcg gcactcactc cggccatgat
    64501 gcccagcgtt gcaatcacca tcaggacaat gtgtaataaa aaaagcatga tatcttgttt
    64561 ataaaacgcg ttgaaaaatt aaagcgtaaa ctgcatggat tggccgacgg ccacacagtt
    64621 cattttcccg ccttcttttt tgacggtgtc gcggcagtcc ttcagaatcg catcgacatc
    64681 ggcgtcggtc ctctcctgat tgtgatgaaa cagtcccagt tttttcacgc gggccccaag
    64741 cgacagtttc aggacatctt tataggaaga atgtccccag ccgcgggttt ttttgtattc
    64801 tgcctcttta tattccgcgt catggaagag cagatcggca tttcggcaaa atgccagata
    64861 ttgcctgaac gggagtcctc cggggtgtct gtgacccagt tcattgtctg tgagaaagat
    64921 gaacgttttg ccgtcttcct cgaatttgta accggttccg ccgttgggat gactcagggc
    64981 aatgggggtg atgagcatat cgcccagccg atagacgggt ttgaaacagg tttcgtggta
    65041 tacaatcttt gcacgaatgt gactgtaatt gaccggaaaa ttaggagccg ccataatccg
    65101 ggaaagcatt ttttttatcg aggtctgggc gaagggacat ccccggatgt ccattcggat
    65161 tttagaggaa tagatgggtt tgaagaaggg aaatcccatg atgtgatccc agtgggcgtg
    65221 cgtcagcaga agcgtgaaat tcaaactgcc ctccgccagc agggcgttgc ctgcctcgcg
    65281 gattccggag cctgcatcga caatcaatat gttgttgtct tttgtcctga tttcaaggca
    65341 ggtggtattg ccgccgtagc gctgatacag tctgcccgat acagggatgg accctctgga
    65401 accccaacag cgaatgatca ttgttaatcc tgtaaaaaaa gatgaaaatg tcgcctatca
    65461 atatccccgt ggatcacgat ggaggataat ttttttctat aggggtttca ataatacaga
    65521 atacatttta aatcaagcag gatatcagga caaaacgttt ctgggaaaat cccgaaagaa
    65581 ccctgtttca ccttgctatt atcaaaaaaa ttattaaact aaaatgaata tagggagggt
    65641 gatgaaatga aaagcataaa aatcaaagga atgagttgcg ggcattgcgt catggcggtg
    65701 acgaaggccc tgaatgctgt ggatggcgtc cggaatgttg aagtggattt gaaaaacggt
    65761 gtggcgactt atgaggagat aaaacctgtc gattccgctg tcgttgcggc agcgatcaaa
    65821 aaagcggggt atgacgttgt tgaataaaac gcctgcggac ggggggctca agaaaaccgt
    65881 tgttgccgtg ggcggcatga cctgcgccgc ctgtgtccgg cgcgtggaaa atgccatcaa
    65941 agacgtaatc ggtgtgcgag acgcggttgt caatcttgcc aacggacatg caaccgtcat
    66001 gcattcgacg caatgggaag gcccggcggc gcttgccagg gttgtcgccg aacaggggta
    66061 tgaatatttg ggcgaaatca aaaatcatct tgccgatccc atcgaagcgt cgcgtcttaa
    66121 agaactccgg gaactgaaac tgaaagttgc ctgtggagcc gttttaagtg tcatcatttt
    66181 tttcggttcc atgcagcact ggtttccttt tctggatttc attccccatc ggatcatgct
    66241 cctgctgctg ttcgttttga ccgccccggc ggtcttctgg gtgggcagcc gcttttttat
    66301 cggcgcctac aaagccgccc ttcagaaaac atccgacatg aacacactgg ttgccgtcgg
    66361 cgctttttca gcttacgcct attcggccgc cgcgactttt tcccctcaat tctttactac
    66421 ggcgggcatt acaccgcatg tgtattatga cggtgcggcc atgattgtca cgctcattct
    66481 gctgggcaga cttctggaag ccggggcgaa gagcaagacg tccatggcga taaaaaacct
    66541 gatacaatta aagccgaaaa cagcgcatct catccgggag ggtcaggaac tcgacgtcgc
    66601 cgtggatgag gtacgaattg gcgatgtcgt ttttgtcagg cccggggaac ggattcccgt
    66661 ggacggcacg atactgtcgg gggaatcgac catcgacgaa tccatgctga ccggtgaaag
    66721 tatcccggtt agtaaagtgg cgggacaaaa ggtttttgcc gccacgatga acacaacagg
    66781 cgggtttacg tttcaggcga ccggcgtggg cgcagaaact gcgctggcgc aaatcatccg
    66841 tctggtgcaa gaagctcagg gatcaaaggc tccgattcag cgtctggcgg ataaggttgt
    66901 ctccgttttt gttccggccg tgtttctgat tgccttgatg acctttgccg tctggtattt
    66961 cctttccgcg gaagggaatt tcagccgtgc cttgatcagt ttcgtttccg tgctggtgat
    67021 tgcctgtccc tgcgctctgg gactggcgac accgacagcc atcatggtgg gcacaggcct
    67081 gggagcgcaa agcggcattt taatcaaagg cggcgaagcg ctggaaaaaa tccataaact
    67141 ttcggtgatc gtttttgata aaacgggcac cctgacgaag ggagaaccgg atgttaccga
    67201 tattttgacc gcggacggtt atgaggaaaa gcagattctg tcgttcgccg cagccctgga
    67261 aaaaagttcc gaacacccgc tggcccgcgc cattgtgcgg aaagccgaac agaaaagcgt
    67321 gagaatcgaa acggttgaaa aattcacggc gctgtccggt ctgggagcca aagccctgat
    67381 ggacggccaa cgtgttctta tcggcaaccg tttcttaatg acagacgaag gcattgccat
    67441 tgatgactgg aacctttcta cggaaaggct gaccggagaa ggaaagacgg ttgtttatct
    67501 ggctttggga aaatcggttg tgggtttgat tgccctggcc gatacgccga aagcatcggc
    67561 tgcgcggacg gtccagcgtc tccagagcag gggaatgaag gtcgcaatga ttacgggaga
    67621 caacgacggt acggcccgcg ccatcgccag acaattgggc attgaaaaag ttatggccaa
    67681 tgtcctgccg gggaaaaagg cggaggaaat aaaaaatctg caggatgccg gcgagattgt
    67741 cgccatggtc ggcgacggca tcaacgatgc gcctgcgctg gcggtcgccg atgtcggtat
    67801 tgccatcggg gcgggaacgg acgtggcgat tgaatccggc gacatcacgt tgatccgcga
    67861 tgatttactg ggcgtaccgg actcgatcag cctatcacag gccaccatgc gcacgatcag
    67921 gcagaatctc ttctgggcgt ttatttataa tgttgtcggc attcccgtgg ctgcgggagt
    67981 cttgtatcct ttctttggca tactgctcag tcccgagttt gccgccgccg ccatggccct
    68041 aagttcgttt tcggtggtga gcaattccct gcggctgcgc cgcgtgtggg aaaagaaaat
    68101 cgcgcgcggg taacggacgg gttgaccgcc tcccggtatc aaaacggttt ttttccaaca
    68161 gaggccagat agatcgtgaa ctcttcctcg ccgtcgatgc tgagcagccg gtccatttct
    68221 tcctgatcat aagcggccag agcgcaggtt ccggcgccga tagcttcgca ggcaaggtaa
    68281 agattctggc agacatgacc ggcatcaagc gcgatgactt tgtgagcggc aataccgtaa
    68341 cgccactcca ttctgcaggg aacggccgtc cagatgaatg tagccgccgc gtctgcggga
    68401 taacgctggc cgaaaaccgc ctgacggatt tttagtttta gattttccac ggtctgttca
    68461 tgcaacagct ggtgctccaa cgggagataa cggtaaatgc cctcagcgat tccctgaacg
    68521 ttcagaatgc acaaatacgt ttccagagcg tgacggcaac cggccgaagg aaccgttctc
    68581 aaggcgtgac cggcgtcaag gatttgtttg attccctgcg ttgcccacag gagaaaagac
    68641 aattctccga gggtaagggg ttgcgtgccg taggttcgcc tgctttcgcg gttgcggatg
    68701 gctgttttaa gatcaagtgt gccgatgttt tgaaactggt catagcgcac aagatcaagg
    68761 cgccgggcgg tgcctgagta gggtttttcg atgggcggag gagggatccc tctgctctga
    68821 tccgtctgag aaaagtcagt gactttacgg atggtatctt tgagaaaaag cctgtaggtt
    68881 ttcagaagtt tttcattcat ggctgcatcc tgtatccgga gccggttttg cggccgggtc
    68941 aatctcttct cagatcagca gcgcagcgat aaacacgccg accatcgtga aggcaattgc
    69001 cagtttcacc acaacagaaa tgaccagtcc gaaccaggtg gcggcaccga cttttcccgc
    69061 atgcaacata tcccgacggg cgatgaattc gccgatggcg gccccggcca gcggcatgaa
    69121 taaaagcccc cagaatccgg taaacaagcc cgcgagggtt cccaggactg caccgatcag
    69181 tccctgccgg cttgcccccg cgcgcccggc ggataacgct gcggcaacgt aatcaatcac
    69241 cacaccgagg accgtcagga tcaccatcac cacaactgtc gctgcgctga tgcgctgaaa
    69301 atcgtcgatc caggcgccga tcagcacgcc gatcaggatc agcggaacgc ccggaagagc
    69361 cggtatcagc gttccggcga cgccgatcag gatgaacaaa acagcaagga tccataacaa
    69421 cgtcatcata ccatgaaaga ctcaaccctc ttcgaatcga tggaacaatc tttatcgcgc
    69481 gccgtgaagc agttcatcgc ccagtttcgc ctgcttgtcc tgaagggttt tttcctgcaa
    69541 tgctcccagg acgttaatat cggtctgcaa aaccttgaag cggtccggag ttttaatatc
    69601 ccatttggcg atggcggcgg cgattcgggc ctgaaatccg gctgccagtt ctttgaaggt
    69661 gacgatcagc ggaacggcgg cattgtcgcg ttcatcaacc ttcttggcgg tttcacggac
    69721 aatggcgcgc atcgtttcca gcagagaatc cagcgcgccg acttcgcttt gcagcgcttt
    69781 atctttatga atgttgcgtt tgtcgctttc catgatcaga tccatgagct ccgcgcccat
    69841 gcctccggtg accgcgccca tcagcaccgt gttttccgca tcgctaaacg ggatgacgat
    69901 atttgcatag gcggagtctt tttctcccaa gatggcggaa gcggggatgc ggcaattgtt
    69961 taatttgatg ccgccgtgcg gcgcaggttt caggaaatcg aatttaagcc tgggcttgat
    70021 cattaggcct tcatgatcgg cgggcaccag aaaggcgctg aacctcttct gatctttatc
    70081 ccggccggtg atggcgatca cgatgaaaag gccggcaatc ggcgcattgg tcagatatgt
    70141 tttctctcca ttgaggatgt aggatgtatc ctgcaaatcg gctgtggacg tcaagagctt
    70201 cggccgcgcc ccgtgtttcg gttcggaaac ggcaaaagaa accgtcagac ttccattggc
    70261 catcaggggc agataacgtt ttttttgttc ttccgtgccg aaaccggaca tcagatgaat
    70321 ggcgatgatc tgctgataaa gccaggacag agccagtccc atgttgaaac cgctgcggac
    70381 gaatacctct ccggcgccgg ccagttgcgt taatcctcct ccgctgccgc cgaattgcgg
    70441 agagatgccc aatttaaaaa atcccgcttc tcccattttt tgccagatat ccagcggaaa
    70501 atcctgcatc gtctgcaaat cgttgcgtga agcgacgtgt tcaatggcaa aacgtgaaat
    70561 ccagctgtac gattgatcct ttgttgtcgt ctgcatttta taaaaccagt tcctctataa
    70621 aagaaaataa ttggttgaca ttgcggcact gacgcacctc atgacaatgc ggcatgtaag
    70681 acccgatctc gctgtcgccg gaactccaga gattttcctg ctcgggattg agccagataa
    70741 tccgccggca gcgttcacgc atctggccta aaatagcatc ttcggggttc atgtagttgg
    70801 tgcggccgtc gccgacaata atcagcgttg ttttcttggt cagaaggtcc atgtaatcct
    70861 ttttaaaatt gcgcagcgtt tccccgtaat cggttgatgc gttgaaattg atatccggtt
    70921 tttccaatac cagccgaatc gcttcgttga tttcattttt ttcaaaaatg gacgtgacgt
    70981 ccgaaagatg atcaataaac acaaaactgt tgaccttgtc aaagcagtcc tgcagggcgt
    71041 aaagcagatt cagcatgaaa cgcgccgccg cccagacgga accggacaca tcgcagagcg
    71101 tgacaatgcg gcttttgcgt ttcgcccggc tgcggtattt gacgtccacc ggaaccccgt
    71161 tgtatttggc cgatgcgcgc agtgtttttt tgatatcgat gcttcccctg ttgcggacgg
    71221 cgtatcggcg gctgacaata tttttcagtt tgcgcaccat cttgtcgatg gcttcgcgca
    71281 tttcttcaac ttcttgccgg gtgaaagaag aaaacgattt ttcgcccagt cctttgagtt
    71341 tttgctcttc cgtttttgtt ttccgggtgc cggattctcc ctgttgcggt tcgtaaacga
    71401 gcatggaacg ggcggcactt acccgttctt cgaaataggc ggcgagcttg cgcccggctg
    71461 aagcatccgt tttcctgtaa tgatcttcca ccagggtccg ggcgtcggac gcggccttgt
    71521 tgatttgcag catcacatta aggcggttgc ccagcggtcc cagattgaaa cgcagcatgg
    71581 tcacgtcttc ttccgttcga atccggcgca tttcccgtag aagcgccatg gggttcccct
    71641 gcaaaaaatc cactacggca tcgtatatcg gattgtcatg ggggattttt ttaagagcat
    71701 ctcccgtcat gttgatttcg gtggaataat cggccgcctg ccggatatcg gatgtgtcta
    71761 tgcgcatttc atggaagaaa agatgataca gccggtcaaa gtgctgaacc tcgcgcatac
    71821 ttttggcaaa attggaacga agcatcgacc ggaattgctg ctcgtccacc ggattgatca
    71881 gtttcatctg atggaggctg tccaggactt cggatgtcga aatcctcatg cccgacgccc
    71941 tgcagcagga aacaaattgt tgaataagcc cgaccataaa ttacagataa aaataatcga
    72001 ttgttctgtc atttagccgt ccgcatatac gggatcctac tccacgataa gcgttatgtc
    72061 aattcgactg aaggctatcg ttttgctacg ttggggagaa gtcttcagat atcagcatcg
    72121 taaagatttc tcatcccgag acgtcggaat tcggaatgac attttaaact actgcaggac
    72181 ccggttgatg tttttacgga ctttttccat atccgcctga tatttgcaga tgatattcag
    72241 cgtttccgaa acaacttcag gtgtcagttc attgatttgc agagcaatca gcgactgcgc
    72301 ccagtcaatc gtttccgaaa tgctcggatt ttttttcaaa tccagcttgc gaattttgcg
    72361 gattgcgtcc acaagagcgt cgcagagttt ttcttcgatg cccggaatct tcaaccgcac
    72421 aatggaaatt tccgtgtccc gcgccggata atcaatgtag agatgaacac agcgtcgctt
    72481 cagcgcgtcg ctcatatcgc ggtagttatt ggatgtcagc agcacaaagg gaatctgaat
    72541 ggccttgcgg gtgcccagtt cgggaatgga cacctgaaaa tcacttaata tttccagaag
    72601 aaatgcctca aattccggat cggccttgtc cacttcatcc acgagaagca cgacaggttt
    72661 ttccgaactg atcgcctgca gcagcggccg atgctgaata aaacggtcgg agaagaaagc
    72721 gtcttcctgc gcggcaattt gatccaccgc ctctgtaaga gtggcggcgg aagagatgat
    72781 gtcgttgagt ctgtccttga ccatctgggt gtaaaggagc tgtttggcgt attcccattc
    72841 gtaaagcgcc ttggcttcgt ccagaccttc ataacattga agccggatca gatcgcgctc
    72901 cagggagcgg gcgatggctt tggccagttc cgttttgccg acgccggccg gtccctcgac
    72961 cagcaccggt ttctgggtcg ctgccgccag aaaaacgact gtcgcaattt cccgcgacgg
    73021 aatatatccg gcatctgtca aactggattg aacatcgtct acgtttttgt attccataag
    73081 agaatgaatt ccttttcgaa ttgcgcaaag cataggtgat ttatcagggt atgtcaatca
    73141 ggaatcgaga tccaacaccg actcaaggag tatatattct cagcatcaga gagttcctga
    73201 atccgctttg tttcgcgtcg gggataatca aatctactgg atcattcgac cagcgcttcc
    73261 taccgtcaac aacgatcatg aaaaagaatt tttctcagcc atcctttcat gctatgataa
    73321 accaaaattg atttgaaata ggaaaataac gtctctgaaa tgacagacag ccccataaaa
    73381 tcagccctga aagatttttt ctttttctgg aaaccttccc tggcccgaag gctgacactt
    73441 tcttttacaa ttttcggcct ggtgatcggt tacgccgttc tcatttatct cgcggtttct
    73501 tcaaccaaca cgttaatcag acaggcatcg gtttcggtag aagaatacct gacgtacatg
    73561 tccagggaag atctgcgtga aaatcagggc gaacttctta aattcattga tcagaagatg
    73621 accaatattg ttcatatggc aaaagccatg caggtggtgc tgctgcccgt tcaattcgaa
    73681 ctttatttta agaaagacgg gcagtggcag catacgttta ccgatgaaca ggggatcgtc
    73741 acatccgaaa taatcagcaa tcccggactt gccggattgc tggaagtggc aaaaagtcgg
    73801 aagatgacga catcgtcaaa atttttttac ggacgacatg ataaagtcaa tgtgaagatt
    73861 aatatcagcc cggacaaaaa taattatacg caacttctcg cctttgacat ttaccgtgtc
    73921 ggcatcctca agattcttcg ggaaaatatc tacaaggcgt ttttcttttt cgttttgctg
    73981 ctcgtgattt gtcatatgat ggggcatatc ttcagttacc tcctggcgcg gcccatagaa
    74041 caactatccc gggaggcgga ggccatcgca tcgggacaat atgaacgccg ttttacgagc
    74101 aatcgtcagg atgaaatcgg caaactggcg gatgcgctca atatcatggc ttcccggatt
    74161 ctggattcga ccaaggaaag agaaaatatt tttatcggca tcctgattgc tttgacgcgg
    74221 gccattgatg ccaaatcgcc gtggacagcc ggtcacagtg agcgggtgac caaatttgcg
    74281 gaagccatcg gacataatct tcatttcaat gaggatgaaa tgcgcgccct gacgatttcc
    74341 gctattctcc acgatatcgg gaagattgcc gtacccgaac agattttgga taaaccggga
    74401 aaattgaccg acgaggagtt tgctgtcgtc aagaagcatc cgcaggccgg cgccgatatc
    74461 atttcttcca ttccctccta tgaatcgatt cttgaaggca ttgtgcatca tcacgagcgt
    74521 tgggacggaa aaggatatcc caaagggctt cacgaaaaag acattccttt aattgcaaga
    74581 atcatttgca tcgccgatgt ttacgacgca ttgacggaag accggccgta ccgcagggcc
    74641 tggagccgaa cagaggtccg gcagtttttt gaagagcaga agggaaaaat gttcgatgcg
    74701 gagcttgtgg atgttttcct tacccttccg gatgttcagt aaagagatga tcaaaacaga
    74761 ataatttatg ttgcggcgga ggggctgctc gacggaaatc ctgttaatgc gcatatgggg
    74821 aaaacattga aaaatgtttc ccccatagtg atacttgaac aaagtggcaa aggtttattt
    74881 ttctttttca aattttgatt tatccgcgcc gcagatgggg caggtccagg aatccggtaa
    74941 atcttcaaat tttgttccgg ccgcaatgcc gttatccgga tcgcctttga ccggatcata
    75001 aacatagccg cagacgctgc aaatataaga cgctacggtc tgaggagccg cgacggccgg
    75061 ttcttctttg gaatatgtcg gagcggtttt cggcgattta cctcctttaa cctgctgata
    75121 ataagcgtag gtcatgggta cggcgtcgct gatcatgtcg cagtcgacaa tattgcccat
    75181 gaaaatggta tgagttccgc aatccatttc tttttgcacg tcgacttcga tgtaggatac
    75241 gctattgtcc agaacgatcg gcacctgggt cacgccggtt tttaccttta cattctgtaa
    75301 tttattgacg tcacggccgc ttttaaatcc gaacagaccg atcagcgtca tcggcgccgc
    75361 ctcggacaag atcgaaacga caaatttccg gctttctttt atcaatgcat gcgtgtagtt
    75421 ttccttattg atgctgatgg ccacggttgc cgggctggag gtaacctgaa acatggaatt
    75481 ggcgatctga ccgttgaatt taccgtcctg accggacgtg acgatgtaaa ggccgtagct
    75541 gattttgtga agagcggatc tgttcatgaa tgttctcctt gtgcaacgtt attcataaat
    75601 tttatagcct aattatgcat ttcgtgccat tgaaatgtca aaaagatttt gcatccattt
    75661 ttcttttgac aaaagaatcc tttttcgata gtggatgcaa aatagaatta aaataaaatc
    75721 ttacgttttt tgttgttgac ctttcagcaa agcgggaaac cattatgaaa tatcaggaat
    75781 ttttaaagag agcccggttt gattttgaag aaattctggc gtttgcttac ggcaagcttg
    75841 tcgacgatcc gccggaacat tttgacgcga aatttccggc gccgcctttt ctgatggtgg
    75901 atcgcatatt gtccattgaa agtgacggca agaaaggtaa aataattgcg gaacaggata
    75961 tcagaccgga cgcctggtat tttcagtgcc atttccaggg cgatccggta cagcccggat
    76021 gtctcggggt ggacggatta tggcaattgc tgggtttttt ctgcgtttgg aggggtgcac
    76081 tgggaacagg cagggcattg gggtgcggcg atgttgcttt caacggtcag attcgccctt
    76141 ttaacaaatg tgttcgctat gaagttgatg tgcgtcgtta ttcgatgctg aaggaatccg
    76201 gcgcaagcat cgtcatcggc gatggacgcg tttatgtgga tcatgaattg atttacactg
    76261 tcggacaggc ccgtgtcggt gttttcaagg atattgccta caaagattac ccgcgccgtt
    76321 caaaatattc gatcggcgga atcatggaaa ggtaatccgt ctgatcatca aggctgttct
    76381 tccggttctg ctgaaaaata tcattaaaat attgatcgtt ggttttttag ggaaatattt
    76441 tgaatatcgt taaaaaaacg gcggcctatt ttgcccgaaa agaattctgc cggaacgcca
    76501 ttaacgaatg cgcggatttg agtcttctga aagagaaaca cacccttggc gtgaccatcg
    76561 gaatcgttct gattgtttta agttacatca tcgttctgcc gttgttcttc atggtggggc
    76621 ttatggctgc caggttgcga aaaccgatgg ttggtgttat aggcattcct ctggcctacg
    76681 gtttttcgtg gctgatcatg atgctgggga tgtatcttac cggccccgaa tatgcgaaaa
    76741 cgctcggaaa atgtgtggtg agagtggtgc tggagaaaat tctcggtgac gaagcgaaaa
    76801 taatcgcctc caagccgaat aaaaatatcg acatgacagg agatgcgaat gagtaacacc
    76861 gaagaaatga taagagaact gaaaatcaaa attatcgaga cactggggtt gattgatgtc
    76921 ggccccgatg acatccagga cgatgaacgg ttggtcggcg gtgatctggg aatcgattcc
    76981 atcgatgtgc tggaactggt catgatgatc gaaaaggatt atggcgtcaa gatcgatagc
    77041 aagaaactgg gggtaaaggt tttcgcttcc gtcagcgcgc tggccgatca tatcctcaat
    77101 catcagaaaa aaacggttta atgtcattgt gcgcaaaaca accactattc ctatgatgaa
    77161 cgtgctgttg atttccgcca atactctgaa aaacccgtat ccggtgtatc cgctggggct
    77221 ggattatgta gccggttgcc tggattaccg ctaccggaca aaaattgtcg atctgaacgt
    77281 ttttccccat ccggaaacac tggaagcaat gatcagggaa ttttctcccg atgttgtggg
    77341 aatttccatc agaaacatcg ataataccga tgcgatcaac tgtcgcagtt ttcttaccga
    77401 atacgagacg ttagtcggcc agatcagaaa aaattccagg gctaaaatca ttctgggcgg
    77461 cagcggcttc acgatttttc cccgcgagtt catgcaggcg ctcgatgccg attacggaat
    77521 cgtgggggaa ggcgaaagac ttgcactgct tctggacgcg tgggagaaaa atgaggatgt
    77581 gaacaaaatt cccggtgtcc tgaccagcgt taaaacggat attgtctacg aaccttttgg
    77641 cggaagactc aacagacatt ttgatcccga agaaactcac acgcgctttt atcttgcgta
    77701 cgggggaatg ctcaatctcc agacaaaacg gggctgtccc ttccgttgcg gctactgtac
    77761 ctatccccat atcgagggcg gtaagatgcg atgctatgat ccggaggaga tcgcccgcag
    77821 cgcccgcgca ttacaggatg ccggtgccaa atatattttt attaccgatt ccgccttcaa
    77881 tgccagctac gaacacagcg ctcaggtggc gaaggctttt acgaaggcgg gcgtgtcgat
    77941 cccctgggga ggtttttttg cctccaccgt tcctccagcc gattacttca agcgtctggc
    78001 ggatgccggt ttgacccacg tggaattcgg cacggaatct ttatccgatc aggtgctgga
    78061 gaatctgcgt aaacccttca cgtcggaaaa tgttttcacg gcgcaccgcg aagcgctgga
    78121 cgccggatta tatgtggcgc attattttct gctgggcggt cccggcgaga acaaagaaac
    78181 gctgcaggca actctgatgg gaatcgataa gctggaaaga gcggtcttct ttttcttctg
    78241 cggcattcgc atctacccgc acacggcgct ttatgatgtt gccgtgcgcg aaggccagat
    78301 caaagcgggg gaaaatattc tgccgcccgt gttttaccgt tctcagggaa tccgtgttga
    78361 agagattatg aagacggtgg aaaatcatgc caacggccgg ttgaactggc tgatcggcgc
    78421 cggcgaagcc aaggccacac gcatcctgcc gaaactttac agacgcggat tcaccggacc
    78481 gttatgggag tatctgattc agtaaaaata taacttcgta aatatttaaa aacgtccgga
    78541 ccgtaactca atgctgcttt ttttgtcctg cccgcagagg cgggcgaagt gcccttgaga
    78601 gtgactccgg aaatatgaga ggctgaaaca tcagaattgc agggactgtt cccctgacag
    78661 ttcaaaacgg cgcattcacg gaatgcacca tacccgtgta gcggttatcc atgaaaaaga
    78721 aattgtcatc tgcaaaccct gctgaaatga ccggagtcat tcttttgctt gccgcggctg
    78781 tgattttttt ctttcatcca ctggccgcgg cagccgtcgc tcttttttat attgttctgt
    78841 gcgttgccgc ttcgtttttc ccgcaaagca gtttctacct gcctgtcatc agccggggga
    78901 gaaccggcag gaatgtcgtt gctctgacgt tcgacgacgg accggccgaa ccgacgacaa
    78961 gacggattct gtatctgctg gatcgctatt cggtcaaagc gacttttttt gtttccggcg
    79021 tcaatgcttt gaagcatccg gacatcgtca gggaaatcat ccgacgcggg cacaccgtcg
    79081 gcaatcattc attccatcac aatccgtttt tgatgttggg cagttatcgt tatctttata
    79141 acgaagtatc caaagctcag gacatcctga aagacagggg tgtcaatacg ttgattttcc
    79201 ggccgcctgt gggcatcatc agccctaaac tgccgtccgt cctggataaa ctgggaatgt
    79261 tttgcgtgac attcagttgc cgaggcggtg atttcggcaa ccgtcgcatt agaaatttaa
    79321 gttcccggat attacacagc gtcaaagcag acgacatttt attgctgcac gatgcattgc
    79381 cgcgcggcgg gggaaaccag gctgttttat tggtcgaaat cgaaaagata ctgcgggggc
    79441 taaaaaaaca ggggttaaac gtcgttcccc tcgccgattt aatcggcagg cacgtatcca
    79501 taatgatgta agatcgcgtc atctataatt aattgttgac cttgtaaaaa atttttgatt
    79561 gaaaaagcaa acaatgatat gcagttatac tttttggccc cgaacgtatg ccgggcattt
    79621 gccaatgagg tatattgatt gaaaaatcaa tggaatttgt taaaagaaac aagagaagct
    79681 gattctaatg atatcgaggc cttagccgat gtcccggcag actccccctg gttttcaggt
    79741 cattttccgg gtgagccgat tcttccgggc attgctctgg tttatgcggt aaagcaggcc
    79801 attcaccggg aagcgcaaaa gaaaggcgag cggcttgaat tgcatacttt gaagagaatc
    79861 cggtttatgc aaccggtaag gccgggtgat cagttatcgt tgaatatccg tgcggcagag
    79921 gcggatggag aacgcttgtt tcattttaaa attttttgca gagatacaat ggtcagcagc
    79981 ggaatgattg ctgccaaaaa aatgatgtga taaaaataaa tataaatatg catggtaagg
    80041 agggaagtga tgcctgcaag tcaagatgtt aaaggcatta tacggcgtgt agccgaaatc
    80101 attattgatg agttgaagct ggaagaagcg accgcggata cctttgatcc ggatatggat
    80161 ctggtggatg aactgggtat tgacagcatg gacctggcca cgatcgcact ggttcttcag
    80221 gatgaataca gaattaccat agacgaggat gattatcccc agttgaaaac cattcgtctg
    80281 atttcagaat atattaaaga aaggcttccg gtcagacatt aattgacaga atgcgagatg
    80341 gccaatcagg taaacaacaa ggtaaatacg gagtccgtca aaccgaaatt ttccgagctt
    80401 ctagcccatc ccgaagtcaa agcgcgctgg gagaaggtga gaaagtattt ttttctgcgc
    80461 gaatcgactt acgatatgac caaccgctgt aatatccgct gtgacggctg ctattattat
    80521 gaaggcgaaa agcaattcgc caaagaggtc ggcgatgtgg aggcctggcg cgcattgatg
    80581 agagcggaaa aagcacgcgg tataacctac gtcgttctgg cgggcgcgga accttcgctt
    80641 gttccggaat tgctggaggt ctgttatcag gaaatgccgt tgggcagcat tgccaccaat
    80701 ggcttcagaa agattccacc gtccgtcggt tataaactgc acatctccgt ctggggcagt
    80761 gatgaaacca gcctgaaaat cagaaaggcc aaaaaccttc tgaaaaaaca gattgaaaat
    80821 tacgaaaacg atccgcgcgc ggtttttgtc tatacattta cgaggaataa tattgatgag
    80881 gttaacgaag taacggaaga gctggccgcc catgactgca aattaacgtt taatgtgttt
    80941 tctgcgccgg tcggatatgc cggaaatctc cggcatgatg atgcatcgct tgagcaaacg
    81001 agacagacca tgattgagct tttaaatcaa tatccgcgca atgttctttt ttccgtttac
    81061 aatgccatag cgcatacgca tcgaaaaggc ctgcacgatc tttacagctg ttcctatccc
    81121 cgtcagaatc cgtcgcagga tatcgggctg ggcagatctt tcaggcagta cagaacggac
    81181 ctgaactggg accggtcggt ggcctgctgc gtaccggata cggattgcgc ggactgccgc
    81241 cattacgctg caggctccgc agtggtgacg gcgcgactct accgccacgt gacgaatctg
    81301 gccacattca aatcctggct ggattatgtc gatacgtatc tggcggtctg ggtgatgaac
    81361 tatgagaagg gtgagaatct ctgtaatgaa atcatagaac cgccgcgnnn nnnnnnnnnn
    81421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    81481 nnnnnnnnnn nnnnnnnnnn nnnnnnntga tgaactatga gaagggtgag aatctctgta
    81541 atgaaatcat agaaccgccg cgcgcggcgg ttgaaaagaa acaaaacgtg ccattgcacc
    81601 cgaaatgaca tccggatggt atgtaaagtc gcaatgatat gatgtaggat agtgaagatg
    81661 aaaacagtca gttccctgct ggacaaagaa tggcacgatc gatacagaag gatttccaac
    81721 ctgaatatcc gcagttccat ctatgatgtg accaaccggt gcaatttacg ctgcaagggt
    81781 tgttttttct tttcttcggg cgagcatcag gcggcggtcg aagaaatgga tatcggaaaa
    81841 tgggaagcat ttatcgatcg cgaaaaagaa aggggcgtga acctggcgat tctgatcggc
    81901 ggtgagccga cgctttgtct ggaccgcgtc gaggcgtttt ataaacggct gcccacgtat
    81961 tgtgccacca acgggttgat taaagttccg cgtgacagat tccccgatat gatggtgggc
    82021 atttcgcttt ggggcagcgc tgaagatgaa aaaattctgc gcggacgcga cacgtttgcc
    82081 gtctccagca aaaattacga aggggatgcc tatacctatt atctttatac cattacgccg
    82141 cggcaggtcg gcaaaattga accggtcatt aaacgcattg ccgatgtcgg attgaaagtc
    82201 cacttgcaac ttttatccaa tgacgagggt gtcgacggtt tttcctggca ggagggcgag
    82261 ctcaaagatc tttgtcacga aatggatgag atgctcgaca cctatccgca gacggtcatt
    82321 tcctgcaaat actatcatga aatcattacc accgggaaaa tgatgggacg acgtttcggc
    82381 tggaatgaat gtccgtccgt taccgaagcc ctggataaca gagcaaaccg tccccgccgg
    82441 ctgattcatt ttgcccgctg ggcatccgat ctgaaaaccg ttcatcgctg ctgtacgtcc
    82501 gcgacacgcg attgctccac ctgcaaagac ggcgcggcgc atatgagctg ggtcatggtc
    82561 aacaagcgtg aacatctgaa atcaaccaga gatctgcaaa actggatcga agtttacgag
    82621 atgtttgcca agctctatca ctttattccg tggtaatccc ccgaaaattt tgatataggt
    82681 ccctctatca aaatccccct ttaatttatt ttcaggggat atcgatttat ttttaaggtc
    82741 ggagcatggg gttgaaaaaa accgtcatcg tgggttatga tgccgtatcg tcactggggg
    82801 cgcagctgga tgcacaatgg cggcaagcgg ttgccggaaa aagcggcatc ggccggttga
    82861 ccagattccc gctcagtgaa aattttccgg tacgcgtggc cggcgaggtg gaggaaatcg
    82921 atccgggtcc gtatccgttt ttgcagccca gagaaatggc gcactggacg tcacccattt
    82981 tcaaatacgc gctgctggtt gtgcatcgcg ctttgcagaa cagcgggatt gaaattacgc
    83041 gcgacatcgc gccccgagtc gccgtcacat tcagctcggc ggtcggggga ctggatgccg
    83101 ttttgcaggc cgatcgcctg atggtgaagg acggcaagct gcctcatccg tttaccaatc
    83161 ccaattcctg catcaatatg gtcggcggca agatctccat tctcaccggt gcaacgggac
    83221 cgatttgttc aaccatcacg gcctgtgcca ccggcattac gtcgatgatc attggcgaga
    83281 tgctgttgca gcgaaatatg gcggatgtgg tgattgccgg tgcggtcgat tttccgctgg
    83341 tggagccgat tgttgcgggc tttgccacga tgaacggcgc ataccggccc aaagaaggac
    83401 agcctgaaga accgccggaa aaaaccagca gaccgttttc tgcaaaccgg cgcggttttg
    83461 tcgtttccga aggagccggt tgcattattc tggcaacggg cgaatttgca aaagtccacg
    83521 ggctcccgtc cggcatggaa atggccggat gggccatgac atcggacgcc agtcactttg
    83581 tgtccccgaa tttggcaact gtgcggaagt gcatcgaaga aacaatagcc aatgccggat
    83641 taaagccgga ggacatcgat gcggttaatg cgcatgcgac gtcaacaaag atcggcgata
    83701 aagtggaggc cgatgctttg aaaaatattt tcggcggcca tgtgccgccg gtatcggcga
    83761 ataaatctca aatgggccat gcgatgggcg cctccagcgc cattgaagcc atcctcgcga
    83821 tggaaggcat gaaaaaagag attgttttgc cgacgatcaa ttatcgtcct gatcaggaca
    83881 ttgcaataga ctgcgtcgcg gaaggtgcga ggaaccttaa acaggaattt gtcttgaaaa
    83941 atgcgttcgg cttcggcgga tgcaattcct gcatcatctt gcacagaaca gaatgatgat
    84001 tgtcatggcg agacaatccc tggtcgatat ggcaatttaa agaaaagatt gctcaagaca
    84061 cccgaaatga cataaaaaag gcaaattatg agagcgccca aaaacagaag agtttttgtc
    84121 gtcggttacg gtgcagccac accgctgggc agcacgtttg aaaaaacgtg gaagagggcg
    84181 gtgaaaggcg aggccggttt ccgcaaggtt acccgctgta aagtggaatc cgcctgcgac
    84241 gtggtcggag aaatccccga ctggtatccg atggaacttg attttaccga cgccaaagaa
    84301 gtttataact ggaacgcggc gtttgtgatc ctgacgatgg ccgtttgcaa ggaggcactg
    84361 gaaaatgccg gtatcgttgt cgacgcacag attgctccgc ggatggcctg tctgatcggt
    84421 tcggcactca acggctcgga tgcttttcgc attgccgtgc atgatctgga tcaccgcgga
    84481 ccactgcggg tcagcccgta tctgcttccc aatttgtgcg cgaatcttcc ctccgggaag
    84541 gcaggcatgc tgttgaaatt taccggaccg attttttcgc cgcaaggcgc ctgcgcctcc
    84601 ggcaaccatg ccatcggcat cggggcgaga atgatccggg acggcgattg cgattttgtt
    84661 ctggccggcg gcgccgatac gcccattctg ccggagctga ttcacggttt tgccaatatg
    84721 aatgccacca taaaagttaa tcctaaagat cgggcatatg ataatcccgc actggcttcg
    84781 aggccattca gtgctgatcg taagggattt gttctatcgg agggagccgg cgttgttgtt
    84841 ctggcggcgg atgaggcgat caaaacctat ggcctcaaac cgaaagccga agttctgggc
    84901 atcggctgga cgtcggatgc tcatcattac accagcccca acatgccgac aatcatccgt
    84961 gccatccggg aaacgattga cgatgcggaa ctggagccga cagatattca atatatcaat
    85021 gcccacggaa cctcgacacc gaagggggat accacagaaa taaaatgcct gcgcgaggtg
    85081 ttcggcaaaa aaatcgaaaa aattcccgta tcgtcaaata aatcgcaact ggggcatacg
    85141 ctgggagcaa ccgcggccat tgaagcggcc ctgacgattg aaggaatgaa aaaaggaatt
    85201 attttaccga ccatcaatca cgtgcccgat ccggaatttt ccgacattga tgtcgtaccc
    85261 aatgaggccc gaaaaaataa atgtgacatc gctctttcca acgcattcgg atttggcgga
    85321 acaaactgct gtgtcatttt cagaggagtc taagatgaaa cccaaacctt tcaagcctga
    85381 aatataccat aacgatgaac gctacgtgcg cgatctgaca accggccttg tctggcatcg
    85441 ttcagtgtac agggttttgt atatcgatac ggatcgctcg caggtggttt atcattccaa
    85501 ctatctgcgt tattttgaat tcggccgcac gtcgctgatg cgcgatcttg cctatccgta
    85561 caaggagatc gaagacggcg gctatgttta tcccattttc gatcttggca tcagctttca
    85621 tcagcctctt tattatgatg acgtcatgta tatccataca cggccggtca atctggagcg
    85681 ggtgcgtctg cagttcgact atctgattac gcatcaggaa aaaggaaata tcatttgcgc
    85741 cggttttact aaacattgcg ccttgaatct gtcaggcacg cccgtggctg tggatgcgaa
    85801 aacggttcat ctatggaaaa catttccggt ttaaatgaac gccagcggta tccgtttgat
    85861 ctcgagatat atccatatct gcgggatcat cacctggaaa acagagcgat cctccctgca
    85921 gtggaatcgc tgattatttt ggccggagcg tgccgggctg tttctttgca gagcgagata
    85981 aaatgcattc agaaagccgc tttttcaaga tttctttacc tggcgccgga taaccatcgc
    86041 cagccggcgg ttatcgatat cataaaaacg gagggcggca acatgattgc gtcgcttctg
    86101 acgtccgtcc agtcaaaaac aggcacaatc agccgtcatg tcgaacacgc aagagcggaa
    86161 tgtgtgatct cgggttttga agaaacaggt ccgccttctt ttcgctcggt ggataaactg
    86221 aagggcagct gtatcagcat tccgtccgct accgtctatc gcgatctggt tcctttcggc
    86281 attgcctatc aaaacatcat cggcgatttg tcggtctctt ccgaaggcgc tctgggatac
    86341 gtttcaggtg gaaattttga ggctgacgat gaattgctcg gatccccttt tcctctggat
    86401 gctgtcatgc acgctgcctg cgtctggggg cagcgctttg ccggcaaagt ttgcttcccg
    86461 gttggatttg aaaaaagact catttatcaa aaaacggaaa aagggcagga atatctcggc
    86521 cgtgttgttc cggtcagcat aaccagggaa tcgctgattt ttgatgtctg gatttacaag
    86581 gacgacgttc tctatgagag cgtcagcggc attataatga aagatgtaac gaatggaagg
    86641 atgaacccgc cggattggat caaggttaat agttgagagc gaagggtaaa attgacgggt
    86701 atcccgcggc acgacgtgcc tgagataagt tgactagcgc ttcaaacttc gttttacttg
    86761 ttctcagcga gtctcattat gggtaaaaca ttttcaatat ttcttttagc gtatctggct
    86821 ggatcagtta attttgctat tatttttttt aaactgacag gcagaaagga tccccgtctc
    86881 gggttcagcg gcaatgcggg aaccacaaac gtttaccgtc aggcaggcat ggtgtgggcg
    86941 gccgttattt ttctgctcga catcggccgg gcgatgctgg tcgcggtgct agccatgtat
    87001 tatttggaac ccgcatggct cccctgggcc ggttattttc ttgtcctcgg caacagctat
    87061 ccgtgttttc atgaatttcg cggcggaaaa ggggtggcca actatctggg atttacgatt
    87121 cttgtttcac cgtgggcagc gatgctttcc tgcgctgtct ggctgcttgt ttacggacta
    87181 gtccggatgc cttttattgc ttcttttttt atggttttta ctctcgcctg cggacaggct
    87241 tatcattccc attggaattg gggcgcgatc gcaggttctt taatgacatg gatactcatt
    87301 ctgtacaatc atagaataaa tatcatggcg catcgcacgc gagaaaaaga tgtgccacat
    87361 caaaataaat aacggtgaaa tgaaatagta tttaagactt aaagttatac tttaagaacc
    87421 aaccgttttt atacgctgac aatgttcaaa ataaaaataa ttgacgtacc cgctgtcatt
    87481 gactatatta aaaatgtcat ttgggcacag cgcaatatta gaaagcacac ttcagaggct
    87541 acattctttc attcaatgtt gcccgtcaaa aaagcacttt tgtccttgaa ttataaggta
    87601 ttgtaaaata ccgatctgca gaatactatt ttattttgtc tgaatattcg gcgaaataca
    87661 caaaacaggg aaggagaaac atatgaaaat aaatgaagcg gaatgccttg agaaatggag
    87721 ctcaatggcg gcatccagca gcctggtgga ttcttccttg tatgccaaat atgatgttaa
    87781 aagaggattg cgcgacatca aaggcaaggg ggtgctggcc gggctgacgc aaatcggtga
    87841 ggtgcagtct catacgcaga tcggcgatga tgaatctgaa ctcggaccgg ggtgccttat
    87901 ttaccgaggg atcgacataa ccgaaattgt atccgggttt gtcgcggaca aacgcagcgg
    87961 ctttgaggaa tcctgttatc tgctgctttt cggtgaactg cccgataagg aggaactgga
    88021 acggtttcaa aaactgctga tttcttatcg ttctttaccg gatgatttta tccacgactc
    88081 catgctcaaa ctttccagcc gcgatatcat gaatgccatt tcccagagta tcttgtccat
    88141 gtatgttttg gacgatcggg ccgaggatat cgagattccg aatgtcctcc ggcaatgcat
    88201 taagctgatt gcggtgttcc cgttgctcct ggtgtattgt tatcaggcat acgcctatcg
    88261 ttacggcaat caaagcctgg tgattcacaa tcccggacgg cagttatcaa ccgccgcaaa
    88321 tttcctgcat atgctcaggc atgacagcaa attcacggct ctggagcaaa agctgctgga
    88381 tttggcactg gtgcttcacg cggaacacgg cggcggtaat aattcctctt tcacggtgca
    88441 tgtggttacg tccagcggta cggataccta ttccgccatt gccgcagcca tggggtcgct
    88501 caagggtccg cgtcacggcg gggccaatat caaagttgtg cgaatgtttg aggacctgaa
    88561 aaataatatt tccaactgga atgatgaccg ccagatcgaa gattatctga tcaaacttct
    88621 caataagcag gcttttgacc gggccggtct gatttacggt atcggtcatg ctgtttattc
    88681 ggtttccgat ccgcgcacgt tgattttaag ggaatatgtc ggaaaactcg cgcaggagaa
    88741 gggactctcc gaagaaatga aactctatga gagagtcgag agtatagccc ctgaaatcat
    88801 cggacatgag cgaaaaatgt acaagggcgt tagtgccaac gtcgattttt attcggggtt
    88861 tctttatcgt ctcctggata ttccgcctga attgtacacg ccgcttttcg cgatggccag
    88921 aattgccggc tggagtgcgc accggataga agagctgtcc aacggaggaa aaatcatcag
    88981 gcccgcctat aaaagcgtaa cgccgcgccg acactatgtg cctatgaagg atcgaaacag
    89041 caacgagcag aacagatagc ccggccgtga aaacagccgt tgatcatcag cggatttttt
    89101 ctaatgacgc cgcaaaaaaa aatggagaat gattttttat tgagcacgcc tccggaatgt
    89161 caggagtgtt gtctgcggtt ataataaatc gtttagtctt tgatgatatt cttcggacgt
    89221 gtagctgtat tcctgcgtgc cgtaggtttc cacggcgtag gacgccgaaa cgctgcccat
    89281 ttttgcgctc gtcacaatgt ccttcccctg aaccagaccg ctcaacagac cgccacggta
    89341 ggcgtctccg gcgcctgtcg gatcgaccac tttttttaat ttgaccggag ggatggcgat
    89401 gtcgctggtc agcgtggaga cttgcgaacc cagttctccc agcgtggtga tgacggttcc
    89461 ggccattttc agcagcgcgt ttttattcag gccggttttg ctcatgatga ggctgagttc
    89521 gtaatcgttg acaatcagaa tccggcagcc ttctatggcc tgaatcagat cgtttttgtc
    89581 ccacatcggc agagactgcc cgggatccag aatgtaatcg atgcctttgg ctttgcaggc
    89641 acgcggatac tctaccatgt cgttgaaatt gccgggcgag acgatgacga ttgtttcccg
    89701 ggagtcgaat ttgtcaaaat cgatcttaca ttggtatttc attgcgccgg gattgaaccc
    89761 ggtaatctga ttatcggacc ggtcggtggt aatgtaggca ccggcagtaa actcctcatc
    89821 gatgactttg atgctttccg tggagatgcc gttttgctga agccacttga agtatttctg
    89881 gtaatcgtgg ccgatggtgg cacagatgat gggatgttcg ccgagcattc tcagagcgta
    89941 agcaatgttg ccggccgtgc cgccgaattt ttccttcatg ccgtttacct gaaagcagac
    90001 attgagcatg tggattttat ccggcagaat atggtcggaa aaatagccgg gaaaatccat
    90061 gattctgtca tacgccattg aaccggaaat aacgatattc ataatttaaa tcagtccttc
    90121 agattatttt gaaaacatat aggaaatatt gatttttgtg tcaatgacat tgttggacaa
    90181 tattcagtgc gaatcttttc tgatgatgcc gccggctacc gtccgcacat tcttcatggc
    90241 ggtcggaaca tcgatcgtga gcaaacgccg gtctttcatg atcaccttgc cgttgacaat
    90301 gctggttttg acatccgcgc cgcgtgcggc atagacaagt tgagagtaat agtgatagac
    90361 aggcgtcaga tgaggctggt tcatatccac taaaatgatg tccgcttgct ttcccgtttc
    90421 gatggaaccg attcgtttgt ccagtcccag aacgtgtgcc ccgccaatgg tggccatccg
    90481 gcagacggat tcagcattca tgaccgtcgg gtcgagagaa gttactttat gaatttttgc
    90541 cgttgtgtcc atttcccgga acatatccag gtcattgttg ctggcgcagc cgtccgttcc
    90601 aatgccgacg gatattcctt ccttcaacat gtcgggaacc ggcgcaacgc cggaggcgag
    90661 tttcatggaa ctttccggat tgtgtgaaac ctttactccc agatcggcga aaattttcat
    90721 atcttctgat gttagccagt tgcagtgaac ggctacggtt tgttcgtcca gcacgcccag
    90781 gttcagcaga tgctggaccg gtgtccggcc gtagcgctgc cggatggtgt ggacttcgtc
    90841 cttgttttcc agtagatgaa ccaaatataa aataccgtgt tcgcgggcgg attgcttgac
    90901 tgcggagagt gttgcgggag aacaggtata gggcgaatga cagaagaaag ccggagtcaa
    90961 gagcggcgca tggggatacc atttctttac aaaacggttg gctgccgcca tcatcttttt
    91021 tacggccgcg ctatccggca tgacaaaatc ggcaaagccc tgggcgacga cggcgcgcat
    91081 cccggcgaca tggacggcct cggcgaggct gtcttcaaag aaatatccgt cgcagaaggt
    91141 tgtcgttccc gacagaatca tttcggccat ggccagcgtt gcgccgtcat acaccatttt
    91201 tttgctgacg aaacgctgtt ccgccggcca gatatgaccg gtcagccatt ccatgagagg
    91261 cagatcgtcg gccagtcccc ggaaacagac cattggcaga tgtgtgtgtg tattcaccag
    91321 gccgggcatc acgatacagc cgtccgcgtt gatggtttcg tgcgcccgga tggaggtaga
    91381 ccgggtcttt ttcagggaac ggatatcggt gatgatgccg ttgtgaatgc cgacaaaaca
    91441 atcctcgatg atgtccatgc cggcggacat ggttaacagt gtgccgccgg aaatgatgat
    91501 gtcgaaatta ttttccggca tttcctgctg cgggcaccgt caatgttgcc gtcttgcttc
    91561 cggacatgtt gcaaacaacc gttacatcca gcttatcgcc gggagcggca gccaccttat
    91621 atacgtagct gaacggcacc tctttgggtt gcgatgagta ggcgttttga ctgacggaaa
    91681 ccgcattctt tttcacgtcg acatttttaa tgtgatgaaa tccggggaac gcggatttat
    91741 gcgtgatcgt gaccgtcagg gtttgcgctc cggaatcgta ttcgattttt acatccttgg
    91801 gcgcgtcggc gtaacagacc tcagcggttg aaaagcaaat cattgataca agaaacaaaa
    91861 aactcaaagc aaaagcagag atgacaattt ttcttttcat ggcagacctc ctgatcttta
    91921 atgttctgtg ctgttcatgt taaatgataa tatattcact gcgcaaataa cagcgaaaaa
    91981 ttatgagttg aaaagttgcg gataattggc tagtgttgaa aaaaatggaa cagtacaagc
    92041 caacttcaac tgccgaaatg aggtgcttat gagcgcagtc atccttgccc ggttacagtt
    92101 tgccctgacg atatgttttc attttctttt ccccgccatg acgctgggca caactctgat
    92161 tatcctgata tccgaaacgc tttatctggt gaagaaggac gagacgtata aaaaaataac
    92221 ggattttctg gtcaggcttc tggcgttgat ttttgttgtc ggggctgcaa caggtattgt
    92281 catggaattt tcattcggca ccaactggtc gggatattcc cgcgcggtcg gagacatttt
    92341 cgggccgatc ctggctgcag agggtgtatt ggcgtttttt ctggaatcgg ttttccttgg
    92401 cgtactgctt tttgcccgca acagagtgtc cccgaaagta tactggttat ccgctctgct
    92461 ggtttttatc ggcggccatc tttccgcctt ctggattgtg gtggccaatt cctggatgca
    92521 gacaccggca ggttatacca ttaatgaagc gggtaaaatc attttaggaa attttggcga
    92581 cgcggttttt aatccgtcaa cggtcgtccg gtatgtccat acggtcatcg cttcatggat
    92641 caccagttcg gtgatggtgg cgggtattgc cgggtattat gtgagaaaag gtctccacgg
    92701 tcagacggcc agaaccatgc ttaaaatcgg gatcatcctt tttgctctga caccgcttct
    92761 gcaattgggg gcggggcatg cccacgcggt gaatgtcatc gatctgcagc cggaaaaagc
    92821 agccgcgatg gaagggcatt ttgaaacagc gcaaggcgcc aagatatatg ccctgggtta
    92881 tgttgatgag aagaaccgca agacgtatgg catctacatt cccaaggggc tgagttttct
    92941 ttacaatttt gactggaact cggaaataaa aggcctcaat gactttccga aagaagactg
    93001 gccgccggta aactttgtgt ttcaggtgta tcatatcatg gtgggtgccg gagtcgtcat
    93061 gatcggcctg ggattgctgg gggcgtatct gttatggaag ggtcgacttt atgacaataa
    93121 atggtatctt tttatgttgc ccctcctgat accgctgccg catattgccc atgagaccgg
    93181 ctggatcgca gcggaagtag gacgccagcc ttggattatc tacaggctta tgaagacctc
    93241 ggatgccgca tcggtggttg tttccgcggg agaaataacg ttcagcctga tcatgttttc
    93301 tcttatttat cttctgttgg gagtgatgtt tatcaaactg gccatcacca tcgttcggaa
    93361 gggaccggcc gcttaatgaa aaaattctaa aaactggaaa ttattctgga ggcaacatcc
    93421 atggacaata ttcaatgcta tcaggagctc tggtttgttc tgatcttagt gcttctcatg
    93481 gggtattctc ttctggacgg atttgatctg ggcatcggag cgctgcttcc ttttttgggc
    93541 aagaccaaag aagagaagga tatcttaatc aattccatcg gtccggtttg ggacggcaat
    93601 gaagtctggc tgattaccgg cggcggcgct ttatttgccg cttttccgca tgcctatgcc
    93661 acggcttttt ccggttttta tctggcgatg atgctggtgt tgttcgctct gattttccgg
    93721 gctgtttcca tggaatttcg tgcacatgac gaaaaaagat ccctcctttg ggaaaaggcg
    93781 ttcattgccg gaagcgcgct ggcggctttg ttgttcggcg tcgcgctggg caatgtcgtt
    93841 tacggggtgc cgctggatga tcaaatggaa tttacaggaa acttctttac actactgcgt
    93901 ccggtgccgc tgatgttcgg ggtaaccggg tttgccgctg ttttgttgca gggagcagca
    93961 tacgcagtga tgaaaacgga aggtgagttg cgagaacggt cctttaaagc ggtgagtttt
    94021 ctaacggtaa taaatctcat aacagccatg atctattttg gaacgattgc ctttaccttc
    94081 gatgacatcg ttacaaattg gttattctat ctggccttta tgtgcaccgt gctttggctg
    94141 tcatcgattt tcttttccgt caggcataaa aacgactctg ctccgttttg ggaatcgtca
    94201 ttcgccttta tcggcctgtg gctggtcgtg gccgccgttc attttcccaa tctgattagg
    94261 gccagcaatg atcaaagtat gagcttgaca atttataaca gttccaccag tttgttaacc
    94321 ctcaaagtca tgagcgttat tgctctgatc gggatgccga ttgtaattgc ctataccatt
    94381 tttgtttatc gtatcttcaa aggcaaaacg aaatcttcaa gtcattattg aaaggcagcg
    94441 acaatgaatg tgattgacgc ctatcaatca cattggtcag atattttgca taggggtggt
    94501 ccggaagata acggatctga gatgataccc gttgaacctg atctggataa tgccagcgaa
    94561 gggagaatgt ttgttgcatg taaacccttc ccggtatggc ccgtcgggaa gggttttaaa
    94621 tgaaggccaa actccggaaa cggagtgcac cgaagactgc tgatcgaatg atcccctcgg
    94681 ggcgacttgt ttttgtgggg aagtatccct ttaaatattg ctttgggttt tgcaaaccat
    94741 caaggagctg gaagatcatg agaatgacgg cgaaagaaat ttacagatcg atagagctga
    94801 ttcgttccag agcgccggtg atccataaca taaccaatta cgtggtcatg aacaatacgg
    94861 ccaacgcgct tttggcaatc ggcgcatctc cggtcatggc gcatgcgcaa gaggaagttg
    94921 aagacatggt ggacatcgcg tcggctctgg tgatcaacat cggaacctta agtgaaagct
    94981 ggatccgctc gatgttcaaa gccgcgcgtc aggcccgaaa aaaaggaatt cccgtaattc
    95041 tggatccggt tggcgccgga gcgaccaact accgtacgaa tacggcgcga gcattaatgg
    95101 cgcaagaacc gccttcgatc atcaggggta atgcgtcgga aatcatggct ctttacaatg
    95161 agcaatccaa aacaaaaggg gtggacagcg cctcgtcttc cgatcaggcc atagatacag
    95221 cgcaaaaatt aagcgaaatg catgagtgtg tcgtatgcgt gagcggagca acagattaca
    95281 ttatctgtcc gcaaacaatc ttaaaaataa aaaatggtaa ccccatgatg tccagggtca
    95341 caggattggg ctgtacggca tcggcgttat gcggagcttt tgccgcggtg ggagaatcga
    95401 cttttgcttc caccgctcag gcgatggcgc tcatgggggt tgccggggaa acggcggcag
    95461 aagttgccgc cggaccggga agccttcagg ttcatttttt ggatgctctt taccgtatat
    95521 cggaagatga tatcgcgaga cgtttaaagg ttgaggatta gacatgcggg gattatatct
    95581 ggttaccgac cgccgtttgt gcaaagacaa atcggtagag gaaatcgttc ttcaggcggt
    95641 caaaggcgga gtctcatatg ttcagcttcg cgaaaaagaa atgtcgaccc atgattttgt
    95701 tgaagaagcc ctgcgtatta agaagagttt aacgccccat aaagttcccc tgatcatcaa
    95761 tgaccgtgtg gatgttgctt tggcctgcgg cgccgacggc gttcatatcg gacaggagga
    95821 tatgccttat gacctggcgc gcagactgct gggacccaaa gcgatcatcg gtctttccgt
    95881 agaaacgtgg gcggatttta ttgccagtca ggcgcttgat gtcagttata ttggcgtcag
    95941 tccggttttt gccacaccga ccaaaacgga tacgaaaggt gtatggggtt tggaaggtct
    96001 ttcccgcatc aaggcattca gtcgtcatcc gctggtggcg atcggcggta ttcatgaagc
    96061 caatatcagg gaggtcattg aagccggagc accgtgcatc gcggttgttt ctgcgatatg
    96121 ttcctcgccc gatccggaga ccgctgccgg tcggttggcg cgcttaatca atgccgcctt
    96181 gaaataaatt gagttatcca aagacggatg cgcctccgac ggagattgcg cccgcaaagg
    96241 gcaagacgcc cgaatgatgc ggctattgtt ttcttttgat ggattttagc gcgccggcgg
    96301 cagcatattt tacttcagcc ggataggatc ctatgcccag aaacgatttt gattcggcaa
    96361 tttccgagag gatggggatt gcctccggcg acccgatgaa acccagagcc gcacagatcg
    96421 tttcctggaa agagacctgt tgctccttcg tcatcgaaga cttgcctttg agcatatcca
    96481 gcaacggatc caccgcctcc gtacatttga ttttaccgag catttctatc atgtgaagct
    96541 tcagttcaaa atctgcatca ggaagcgcgg ataggagcag tgctccccgg cggtttccgc
    96601 cgatctgacc gatacttttt aaagtttccg cacgcacgcg cttgtcttta tgaaagagaa
    96661 gaggccgaag aatctgaaca ttttcctcgt cgccgatgcg tccgagaata tatgccatat
    96721 tgcggagaaa gtaccaggga gcttccgttg taatgttttc tttaatggcc gggatcgcct
    96781 tctgtccgat ttcctggatg atatgaataa tgcgtatgcg ttccttgctg tctttggcat
    96841 gacggacgat atccagcagt tttgtgatga taaaatcgtc gccgaatccg gaaagaattt
    96901 taaaagcgtc cgttgttttg tttttttcgt tgatgttgat ttccttaaac aggaaatgaa
    96961 tgttgttttc cgaggcaaga tttttaagaa cctgtaaaga gatatcccga atgtcatcat
    97021 ttttttcaag gacgccggtg ttgatttgac ttaaaacaga aataatcgaa ttggcttcgg
    97081 tcagaagttc gtgatgaatc aatgcctgaa ccagtttttg cagactgtcc agatttttaa
    97141 catagtccgg agtgaaaacc gattctatct tcagccactc ggccacgtgc ccggcagatt
    97201 tcctgatcag gttggtttgt tcttcagcaa caaaaggttc gggggcgttt ttcaattcgt
    97261 tcaggcgtct taatgattcg atcgttttgg caataatctc ggaaaccgga tcatccgtcg
    97321 ctatcacgac aggaggttga tttatttcgg ggaggttcgt catctcagga accggctcgg
    97381 gatctgggac gggctggggt tcagtctgat cgccggccag ggaaaattca acatcttgtt
    97441 tatctgcgtg aatatgaata atgccgttat cggttattgt cttggcaagg ccccccaaat
    97501 ggcgcaaatg ttccgggtct ctcgccagag actttataaa agcctgaagt tcatttttga
    97561 gcaggccttt ttcaaaagaa atgctccgaa gaccgaaatt aacgaataaa tccatcagaa
    97621 aaacaacatg aagagtttcc tgatcctttt ccctgagggc ttctccgcac agcatgactt
    97681 ttttccccga ttccgcaaac accagggggg atttctgctt taaggtgtcc agaagataaa
    97741 gataagctct gtcgatggaa ttttgcgtga tgggactgtc ttctgaatag agctggacgt
    97801 ttttaacagc cgcgcaaaga tggatcattg aatccattgc tttcaaatgt gcttctgcgt
    97861 tcacgacgat cttctccccc ttaaataata gtcaggaaaa attcaagctg ttgagaattt
    97921 ccggttcaag aacttcaggc aacagcttga atttcatcat aacatttttg ggaatcgaac
    97981 gcaatcggcc ggtttttgca tcaacaaaaa cccagtcggt tttgccttcg acaagcacag
    98041 ccttgtcttt acagcggatg actttgtatt ttcttaagga ccgtacgcga cgaaaatcag
    98101 aaacccaggt cagaattacg acggtgtctc ccgcgaaggc aggacgcaga tattcgatat
    98161 gatgcatgcg caccacccag gtggctccga catccagggt ggctttggtg catccccgga
    98221 tgtcggaatg aagcacggcg gcccattgca tccattgcaa atattcaacg ttgttgacat
    98281 gaccatttat atccacggcg ctttccggaa ccgtgagttt atattggtag acttgcgtca
    98341 tgatgtaatt tcctgtttga taaaggggaa taataattga catcaattca tgttttatga
    98401 atatagtaaa tatggtgatt ttgcaacagg cttctgaaca taacggcatt gcttgccgat
    98461 gaagaataaa aatttcaatt attgaggaaa ggaagtgcac gatggtaaaa gaaaaagaaa
    98521 gtaatgttgc agggaatctc gaatcttatt acaccaaacc gaataatctg gtcgatatgt
    98581 ttgaagacag tgcggcgaaa tttgcgtccc gcaatttgtt cggcacaaaa aacaaagaga
    98641 ccaatcaata cgagtgggta acctttgcgc aggttgccga gcgggtgaac aatctgcgcg
    98701 gcgcgttgaa aaaaatcggt tttgaaaaag gcgaaaaggt cggtgtcatt gtcggcaact
    98761 cggcggaatg gtttgtttgc gccaacgcca ctcacggtct gggcggtgtt tttgttccca
    98821 tgtacgaaaa ggagctgcag aaggtgtggc agtacattat caaggattcc gccattaaat
    98881 atctttttgt cagcaatcaa aaaatctacg agatcattaa aaattttcaa aatgatattc
    98941 caacattaaa aggcgtgttt atgctgtatg gttcgggaga aaattccctg gcggaactgg
    99001 aaaaaatggg caaggcaaat cccgttttgt cgtacaagcc gcactggtcg gaaacggcgg
    99061 tgattattta tacgtccggt accaccggcg atccgaaagg cgttctgctg agtcacggca
    99121 atctgacgca caacgccaag gaaagtcatt ggacattcga tgtgggggag gaagaggtcg
    99181 ggctgagcat tcttccctgg gcgcacagtt tcggtctgac gaccgacctg catgttttcg
    99241 tctatggcgg ctggcagctc ggactggctg aatccgccga caaacttctg ttgaatttcg
    99301 gcgagatcaa gcccacgcat atgaatgccg taccgcgggt gttcaatatt atttacgata
    99361 aaattcagca gagcgtgtca gcggatccgg aaaagaagaa aatcttcgat gcggcggtag
    99421 ccgaggcgat caaaaaccgg gatctgaaag aaaaaacgga cgaatttaaa ttctatgacg
    99481 cggtcgcctt ttccacggtt cggaacattt tcggcggacg gttaaaacag gtcgtcattg
    99541 gcggagcatt gatgaaaccg gaaatcgcga tgttcttcag cgatgtcggg gtcgttccct
    99601 acgtcggcta tggtttgtcg gaaacatcgc ctgttcttac caacaatagt cccaagcggg
    99661 gcaataaatt cggcacgatc ggcaaaccgt acaaagatac gaaagttgtt atcgataagt
    99721 cccgcgtcgg agaagacagt ccggacggcg agatcgtggt gtatggcgcg caggtcatgc
    99781 agggatatca caacaagccg gaaacaacca aagaggtgat gatgcccgat acctggaacg
    99841 gattccccgg cgtcaggacc ggcgaccgag gctggattga tgaagacggt tatctgaata
    99901 tcaccggacg tttcaaggat gaatacaaac tggaaaacgg caagtacgtt catcccgaat
    99961 ccattgaaaa cgagatcaaa cttctgcgtt acgtttccaa tgttattgtt tatggcgagg
   100021 gccggacgtt taacgtggcg ctggttgttc cggattttga ggcggttaaa accgattcgc
   100081 aaacgtcccg gtgggcgcag ggcacaccgg acgaaaccat tgccaataag gagtgcaccg
   100141 attttatctc caaacagatc accgaccatt tgcgcaagtc tttcggcgga tatgaaattc
   100201 ctcagaagtt cctttatatc acggaggact tttccgtgga taacggcatg ctgacgcaaa
   100261 ccctgaaact caaacgccgc aatgtcatgg aaaagtacgg tgagagactc cgcgctcttt
   100321 atgacaacgt gtaaatcgaa aaacatgata tcatgttcac catgagaaca tgatcataca
   100381 gctcggaata ctctggaggt tttatgaaca atcaaaacga cgtggtcatt gtcagtgcgg
   100441 tgcgcacgcc tttcagtaaa ttcggcggcg cgctcaaaga aattcacagc accgatctgg
   100501 gtgtgattgt catcaacgaa agcctcaagc gcgcggggat gaccggtgag gatttggacg
   100561 aggtttatta cggcatgtgc gatcaggccg aggctgccct ctacgacaac gtcaatgcgc
   100621 ggcaagccat tctgcgcgcg ggcctgccca gcacgctcgt atcgcttacc ttggatcggg
   100681 cctgttgttc gtccctttgc gcggtgcagt tgggccgccg cgccatttta ttaaatgagg
   100741 cggacgcctg catggctgtg ggcgctgaga acatgagcaa tacacctctt ttgatcaacg
   100801 gccatcgctg gggttcgggg atgaagccgc ttctggtaca ggattacctg aatcccatta
   100861 cctaccgcga atacacgccg ctgggacgcg atgcgggcga gattgccatc gaacacggta
   100921 tttcgcggga ggaacaggat gcctgggcgc tggggtcgca tcagaaatgg gatgcggcgg
   100981 acaaggcggg gcttttctat gatgaactgg tcagcgtgga gttgccggca aaaaaaggaa
   101041 cgccggtaat tttcggtaaa gacgaacttc cccgtcccga caccaccctg gaagcattgg
   101101 ccaggttaaa aaccatttac ggcagcccca cggtgacggc gggcaatgct ccggggttgg
   101161 atgccggagc gacggcggtc attatcatgc gtcgatcaaa agcggaaaag ctcggtatca
   101221 gaccgctggc aacgattgtg gctgtggcca gcggtgcggg cgagcccaga cggatggcgg
   101281 aacttccggc aaaagtcatc gctcaggcgc tcaagcgcgc atcgctttgc atcgacgaca
   101341 tgagcgtcat cgaaattaat gaagcctttg ccgccgttac cctggtttca acaaaaatgc
   101401 tggccggtgg tgatgaagcc aagtggcgcg agttgctgaa gaaaaccaat cccaacggcg
   101461 gagccattgc catcggtcat ccggtgggtg ccagcggcgg aagaattttg atgaccatga
   101521 tgtatgaact caagcgccgt ggcggcggtt acggcatctg cggcatatgc ggcggactgg
   101581 ctcagggcga cgcggtcgtg attcgtgtag attgaattcg gcacgggttt tgaggaatgt
   101641 gttgtgtcgc cgtttgttga ggttagacaa tattccgttc gttaacatgt agaaaaggag
   101701 gctcaacatg agagatgaaa aaggcattgc gtcctgggtt gttatcgtaa tcattgtgtt
   101761 gctgattgcc gccggttttc tatggtcaaa atatttcgcc gccccgacgg caacggtgat
   101821 gatgcaaggc gccgatcagg cgaagccggc cattgaaaaa gcgcatcagg cttccgacgc
   101881 ggccgaccgg gccaacaaga ttgccgaaga ggccgcgaaa aggttaaatg aggatgccgc
   101941 ggcgcggcag tgaaaaaaat ctgttcggaa agcagagatc tagtgacatt acaggatgag
   102001 gcgcgttgtg tcttgcggat gttgaagcct gaattcgctt gacttacata tatgaaataa
   102061 ttatactttc ataaaaacaa tgtcggggag taagcgttat caagaatatc gtaatcaggt
   102121 ggaataaaaa tcgttttcac agtctaatga tctttatgat caatatttaa ggaggacgtt
   102181 atgaaaaaag cactatcgtt tgtttggatc ttggtgctgt tggcttttgc cacagtggcg
   102241 caggctgaag ttaaggcagg ttctttttca gtaacccctt tcatcggagg ctatgtcttt
   102301 gaaggtaatg aatatgataa taatgaagac acctacgctg ccggtttgcg cgccggttac
   102361 aattttacaa aaaatatcgg tgtggaagca ttttttaatt atgttccgac caataacaaa
   102421 gactggaatg acgacaatga aatggaactc ttcggatacg gtctggaagg gctttatcat
   102481 tttatgcccg aaagccgctt tgttcccttt tttgctgtag gggtaggtgg tattcattat
   102541 gatgcgcttc ccgaggatcc ggataaccgg gataaatttg ctctcgatta cggagccggc
   102601 ttcaaatatt ttgttacgga caatattgca ttgagagccg atgtccgtca tatcattcca
   102661 tttaacgagc gatacaacaa cctgctttac tcggtcggcc tcaccttcgc ctttggcggc
   102721 gagaaaaaga ttgtagaaac aaaggttgaa gagcccctgc ctcctcctcc gccacctgct
   102781 ccggttgcca agccggttat cattgagaag ggaagacaga ccctggatgt caaatttgat
   102841 tttgataaag caaccattca ggacggttat tataaagaca tcgatgcgct ggttgaagtg
   102901 atgaaagatt atccggaatt gaatgtggtc atcgaaggac atacggataa tgtgggaaag
   102961 gctgaatata ataagaagct ctctcaaaaa cgcgccgaaa ctgttaaaaa atacatggtc
   103021 gaaaaaggcg gtgtcgatgc cgcccggatc gaggcaaaag gctttggcga tgagagaccc
   103081 gtcgatacaa acgatacggc cgatgggcgt gcgaaaaacc gtcgcgtgga agctgcggtt
   103141 gattatatca tcgaaaagta aagaaggatt ataaatcgtt tcaacataaa agagccccgg
   103201 ggatcacttt tcggggctcc tttatatttc ttcttcatga caatgcaggc ttgaaacctg
   103261 atcggaagga tggcctgccg cagtaagacg cccctccctt ttttcatcac gacgatcatg
   103321 caataaaaaa aggcttgaca aatagtgtca ggttcattag aaagcgtaca aacaaagaca
   103381 ggaatctcac gatggacaga aaacagaatg taaatatgaa tgtttgcggt tactggtttt
   103441 atacctattt tatatcggtc tgcccgtcgc tgaggaggac tcgttaaggt tcagtaaacc
   103501 aaaacagcca tgggcggatc gaaagcgctc atggcttttt ttttagaaag ccagggcgtg
   103561 cagacccctg gctttttaat ttcaaaggag gcaatatgat tatcgtcatg aaaagtcatg
   103621 cgaccgaaaa acagattgaa gatgtgattc actggattga atccgtcgga tacaaggccc
   103681 atccttccat cggggtggaa agggcgatca tcggagcggt gggtgacaat cggggtaagg
   103741 atattctgaa atttgcggaa tccctgcctg gtgtggaaaa gaccatgccc attttgaaac
   103801 cctacaaact ggccagtcgg gaatcgcatg atggtaatac ggaggtgacg gtaggagagg
   103861 tgaccgtcgg tggcccggaa tttgtggtca tggccggacc ctgcgcgatt gaaagtgaag
   103921 aacagttgct ggagtcggcc tatgtcgtca aaaaaggcgg tgcgcagatt ctgagaggcg
   103981 gtgcctataa gcctcgcacg tcgccttaca gctttcaggg gatggaggaa gagggcctga
   104041 aggtcttgaa caaggtcggc actcaggccg gtttgccgac ggtgacagag gtcgtcaatc
   104101 cggcggatgt cgatctggtg gaatcttacg ccgatatgct gcagatcggc gcccgcaatg
   104161 tgcagaattt cgccctgtta aaaaaagtcg gtcattcgcg aaaaccggtg ctgttgaagc
   104221 ggggtatgat gaccacgatt gaagagttgc tgatgtcggc ggaatacata ctctccgaag
   104281 gcaatcccaa tgtgattctg tgtgaacgcg gtatccggac gtttgaaacg gccacgagaa
   104341 atacgctgga tatcagtgcc atacctgttt tgaaaggatt aacccatctg ccggtcatca
   104401 tcgaccccag tcatgccgcc gggaactgga aatacgtcat tcccctgtcg cgggcagcgg
   104461 ttgctgtggg tgcggacggc attatcgttg aagttcatcc ggagcccgag aaagcagttt
   104521 ccgacggcgc tcagtcactt aaaccggaaa agttctatca gatgatggac gaaatacggg
   104581 ccattaccgc aatcatgaaa cactagaaga acattttata gtgaagggaa gacatcatga
   104641 gatcgataaa agttaacctg gacagaaaat catcctcctc ttacgaaatc cgtatcggaa
   104701 aagacatatc ggatcgtctg tcgctgctga tcgccaaaaa ccataaggcg gcacgttatg
   104761 cggtcattac ggacaatatt gtcggggaac tttacggtaa aaaatttctg acggcgctaa
   104821 aagagatcgg cctcaacgcg tcgttgattg attttcctgc gggagagtcc tcaaaaacca
   104881 tcaaaacggt tgttgacatt gccgaaacat tagcggtctc cggcgctgac cgggagacat
   104941 gtctgattgc tctgggcggc ggcgttgtcg gagatattgc cggtttcgtt gcctcgatct
   105001 atatgcgttg cgtaccgtat atccagattc cgacaacgct ggttgcgcag gtggacagca
   105061 gtataggcgg taaaaccggc gtggaccttc cttgcggaaa aaatctggcg gggacttttt
   105121 atcagccgca ggcggtcatg acgagcctgc attttctcaa tacgctgcct ccaaaagaat
   105181 tcaacaacgg gctggcggaa attataaaat acggggtgat tgatgatgaa aaaatgttcg
   105241 ccagcctgga agacatcatg acggcggtca aatccaagga ccccaaaata ctgctcggtg
   105301 tcgtcgaaaa atgttgtcag atcaaaaaat caattgtgga aattgatgaa aaagaagggg
   105361 gcgtgcgtcg tattttaaat ttcggccata cgatcggtca ttcgctggaa gccgtttccg
   105421 ggtatgcgat tacccatgga gaaggcgtgg cgattgggat ggtggcggcc gcgcgacttt
   105481 ccgccaggat gggttatctg aaaaacaacg aggccggccg tatcgaaacc ctcattgatc
   105541 aggcgggcct tcccacgaga ataccgaaaa cgctgtccgg cggaaaaatt gtggcacaat
   105601 tgaagatgga taaaaagaga aaaggagccg cgattcattt tgtgctgctg aaaaaaatag
   105661 ggttgccctt tattcatggc aatatcgagc agaaaatgat tgccgacgtg atcgaggata
   105721 tgaaatcatg aaaccgcacc tgctggaaaa cttgcgaaaa aaaataaaag acaaggataa
   105781 ggagattgtc cgacttttga acgagcgggc gcagatatcc gttcagatcg gcaaaatgaa
   105841 ggggcagggg gggatgcagg tgtacgatcc ggcccaggag gcgaaggtcc acggatattt
   105901 acgggacctc aatgccggac cgcttgcgtc cggggcggtg tcggcgattt tcagggaaat
   105961 tatatcggcc tcgcgtgatt tgcagaagcc gatgacgatt gcctttctgg gacccgaggc
   106021 gtccttttcc catctggcgt cgcgcctgca tttcggtgaa tcgagtctct tctttccaca
   106081 gacgggaata ttccgcgttt ttgacgaggt ggaaaagggt gccattgact ggggtgtcgt
   106141 gcccgtggaa aattcgctgg aaggatcggt caacgtgacc ctggacaggc tgatgataac
   106201 gccgatgaaa atcagggcgg agatatatct gcgaatccgt cagtgtctta tgtcgtcggc
   106261 caaaaacatg aaagaggtca aaagaattta ttcacatccg caggcgttgg cccagtgcca
   106321 ggtgtgggtt agaacaaatc ttcccaactg cgtggtatgc gaaacggaaa gcacggcagc
   106381 cgctgtacag atgatccggg gaaagaagca tgaagccgct atcgggagtt cgcttgccgc
   106441 aaagatatac ggcgtgaatc tactggccga aggcatcgag gataatccct ccaacatgac
   106501 gcgttttctc gtaatcggtc agggtgcgaa cgatccgacc ggcaatgata aaacatccct
   106561 gatttttgcc acaccgcatt ctccgggggc gctgcatgcc gcgctgtccg cctttgcccg
   106621 tcggaaaatc aatctggcga agattgaatc gcatcccgtg aaggataaat tatgggagta
   106681 cagtttcttt gtggatatga tcggccatgc agatgacagg cgtgtgcagg gttgtcttcg
   106741 agagctgcag aacaaaacgt cttttatgaa gattctgggg tcctatccca aaagcgagga
   106801 tgcgctatga tttgtattcc cattacctcc gacaacccca aagaggcttt gcatgccata
   106861 gaacgaagct gccagctggc cgactttctg gaactgagaa tggatctgat cggcaatatc
   106921 ggccttgccg aattgatttc agccgttcgt gccgagtctg ctgcagtaaa gattattgtc
   106981 accagccgga aaaaagaaga ggcggctttt gtcggaagat ggccgcggcg tcgaaacgcc
   107041 tgcagcaaaa cgaggtataa aatagcgctg ctgaaggagg ccgtcgaacg gggtgcggat
   107101 tatattgaca ttgagctggc cgaaggaaca caggctatcc ggcaactgca agcgttgtgt
   107161 gcggaaaaag gttcgcaaac caagatgata atttcctatc atcatctgaa agacaccccg
   107221 gatcttgcaa cattgaaaaa aatatttcat cggtgtgccg cgtataagcc ggcagttgtg
   107281 aaaattgtga caacggcgag atccgaagag gataatctta tcatattaaa tcttattgcc
   107341 tatgcgcgca ggaaatctca aaaaatcatt tcgctgtgca tgggcgagaa aggcggcatc
   107401 agccgcgcgg tggcgcctct gatgggtaac tttctgagct ttgctgcact ggaccgaacc
   107461 gggcgctcgg cccccggcca gtttaccgtt cacgagatga accggattca aaaatggttt
   107521 aaaggcaaga acagagcccc tcagtcctca gcgtcatctc cgcatgcgtc tttaccccgc
   107581 cattacgttt tactgggcaa tccggtcgag cagagcctgt ctcccgtgat gcatgatgca
   107641 gccctgaaaa aaacaggtct tgcggaacaa tatgtcgctt tttgcgttca ggatatcggc
   107701 ggtgcaatgg acgggttgag agcgatgaat attcacggcg ccagtgtgac cattcctttc
   107761 aaggttgccg tgatggaata tttggatgat attgatgcgg atgcgttgga gatcggagcg
   107821 gtgaatacga ttgtcaatga aaacggccgt ctgatcggac ataacacgga ttgggccgga
   107881 ttgattttga cgcttaaaaa ggcaatgacg atcagacata aaaccattgt aatcgtcggc
   107941 gcaggcggaa ccgctcgggc ggcggcttac ggcatcctga aagagggtgg atgtccggtt
   108001 attgtgaatc gtacccccaa aaaagggaaa atgcttgccg aaaaaatgca gtgtgctttt
   108061 tatccgcttt ccgagatcgg ccgcatcagg ggggatgtac tgatcaacac aacgcccgtg
   108121 ggaatgtatc cccggggaaa tcaatcgccg gtcagcgccg ccgtattgtc ggattacgac
   108181 tgcgtcatgg atgtgatcta taatccgatt aaaacaaaat tgctgaggga tgccgaaaag
   108241 cagggttgcc gcattgtaac gggtgtggaa atgtttgtta atcaaggcgc tgagcaattc
   108301 cggttatgga ccggcaagga accgccgcat gcggttatga aaaaaatcat tctggaaagg
   108361 ctgagcaaag gtggacgata atcctatccg ttgcccggac ccggctgtga cggtccgcgt
   108421 tccgggatca aaaagcttga gccagcgggc gctggttgcg gcaacgctgg cgcaggggga
   108481 ttccatgatc agcaatctgc ttgtttctga ggataccctt tacatgatcg gtgcgttgcg
   108541 ggaactgggc gcaaaaattg tcgacgcgcc ggatggtttt caggtgaccg gcacggccgg
   108601 caaaattgaa aacagcggca gggacctgtt tctgggcaac aacggcactg ccctgaggtt
   108661 cctgacggcc ctggtctgtc tcggaaaagg ccaatatgtt ctgaccggcg aaaaaaggct
   108721 ctgcgaaaga ccggtgggtg cgctcgggga tgccctgagg gacatgggcg tcgatattcg
   108781 gacacatcat cattgcccgc cggtcacgat caatgccggc ggtctcgccg ggggaaaaat
   108841 aacactgcgt gacatagaaa gttctcaata cgtgtcagcg cttcttttat gcgcccccta
   108901 tacagaccgg ggaatagagc tgagcctgga aggcggcgtt gtttcagctc cctatattga
   108961 cctgacgatt gctgttatgg aggattttgg cgtgaccatc agcaaaccgg agcgatttgc
   109021 ctatcgtgtc aatgccggag agacttatcg gggacgcaga tatgttgtcg aaggagatgc
   109081 gtcgagcgcg tcgtattttt ttctggcggc ggccctgctc aagacaacaa tccgggttga
   109141 gggaatcagc cgggaaagca ggcagggaga catccggctg ctggatgttc tggaaaaact
   109201 gggctgccgg ttccggtcga ccacaaacgg agtggatgtc accgggggaa cactgttgaa
   109261 aggcgatagg gtcttcgact tcggcgatat gccggacatg gttccgaccc tggcggtctt
   109321 gtccgccttt cgcgagggac agaccgttat caacaatgtc gcgcatctga gaatcaaaga
   109381 gagcaatcgc ctggcggctg tcgcaaccga gcttaggcgg gccggtattg atgcccgcga
   109441 aattccggac ggccttgtca taaagggcgg acgcatgcgg ccggcaaaaa tcgaaaccta
   109501 taacgatcat cgcatggcca tgagctttgc gatagccggt cttgttgttc ccgggattga
   109561 catccgcgat aaaaagtgcg ttgataaatc attcccctcg ttctgggagg agctgcgtcg
   109621 tttatgaagg ttgttttaat cggttacagg gcgaccggca agtccaccgt cggcaagatg
   109681 ctggctgccc ggcttaatgt tgatttccgg gataccgatg ccatgatcga agaaagcatg
   109741 gatatgccgg ttaaggaaat cgtcgctctt cacggatggg agtatttccg gtcaaaagaa
   109801 agagacatca tcaaatcgct tgtcaagaaa gaagattgtg ttattgcaac cggaggcggc
   109861 gtggttctct tcagagaaaa tgttgatcta ttgaaacaga acgttgatct gctgaaacag
   109921 gccagtgtca tggtctggct caacgctccg ctgcgcgaca ttgtggaacg gttaaaacag
   109981 gatgcgcaaa acgaagcaac gcgtccgccg cttacatccg gcaacattgt tcaggagacg
   110041 atcgacacca tgagaaaaag atttcccctg tatgagcgat ccgcggatta tgttgtggat
   110101 acgctgagca aaacccccga gcagattacg gaagaaatct atcagtattt gcttaaatcg
   110161 ggaaatttgg ctaaaataac cgtcattaaa aaataaaaag gaaaaaaatg tccggcaata
   110221 cgacaggcag cgtttttcgc gtcacaacct ggggagaatc acacggcagg gctctgggag
   110281 ccgtcgtgga cggctgtctt ccgggaattc ccttgacgga atccgatatt cagcaggctc
   110341 tcgacagacg aaagccttcc gcctccgtag cctctactac acgaaaagag ggggatctcg
   110401 tggaaatcct ctccggcgtt tttgagggta aaacgatcgg tacgccgatc tccctcatca
   110461 tcaggaatgc tgacgcgcaa tcttcttcct atgatgcatt gaagaatgtt ttccgccccg
   110521 gccaggggga ttttacctat ttgcaaaaat acggaatccg cgactggcga ggcggcggac
   110581 gggcttccgg cagagaaaca gccgcccgtg ttgccgccgg agcaattgca gcaaagatac
   110641 ttgatcgggt cggtatcgac gtcatcgcct ataccaaggc aatcggttcc atcgcgattg
   110701 accgcggcag aatggccgca gaaaaaaagc tgagaacggc cgtgcaggaa aacgctcttt
   110761 tttgtccgga tctacaggcg gcagccagga tggaaagaaa aattacggcc gtccgtaaaa
   110821 aaggagattc cctgggcggg atagtggaaa ttatcgtccg cggttgtccc ggagggctgg
   110881 gcgaaccggt tttcgacaaa atggatgccg atctggccaa ggccctgatg agcatcggat
   110941 cggtgaaagc ggtcgaagtg ggtgacggat tctccatggc cggtttgcag ggctctcaaa
   111001 ccaacgatga aataatgccc ggaggattta aaaccaatca tgcgggagga attcttgcgg
   111061 gcatcacgaa cggagaacat atcgtcctgc gggcttattg taaacctatt ccgtccattg
   111121 cgaaaccgca gcgcaccatt gacagcaaag gcaaaccccg gttcattgaa attcagggaa
   111181 ggcacgatat ctgcgttctt ccccgtattg tgccggtgtg cgaagccatg gtgaatattg
   111241 tcgtggccga tcacttcctc agacagagag cgatccaatc atgaaaaaaa ttaacgtggg
   111301 cattatcggc ggcaccaacg gcatgggaaa gtggtttgcc gacctgctga aaaaagaagg
   111361 ctgccgggtt tatgtctgcg gcagaaaaac ccggttgcac ataaacgatt tagccgggct
   111421 ctgcgatgtg gttgttgtcg ctgtgcccat ttccgccacg cctgaagtca tcggacaggt
   111481 cggcccgcaa ctgacaaagg atcagcttct gatggatttg acatcgctga aaaaagaacc
   111541 ggtgacgcgg atgctgaaaa cgtcgcaggc ggaagtcatc ggctgtcatc cgcttttcgg
   111601 tccgcagtta aaagacgtat ccggacaaaa cgtaatcctg tgtccggcgc gcggaaaaaa
   111661 atggctcggc tggctgaaag gcgtttttaa aagaagcaag atggccgtcc gggaatcaac
   111721 gcccgaaaaa catgaccgca tgatggccgt tgttcaggcg ctcaaccatc tcgataccat
   111781 ggtattgggg ctggctattg ccaggacggg aatggctttt gcggacatcg acaaattttc
   111841 cacgccgatt tttcgcatca aaatggaaat tatccgaaaa gtatttacgg aaagtcccga
   111901 actctatctg gatattatcc ggggaaattc tcaaaccatt aaaatgctga acatctatga
   111961 aaaagcattg aaagatattc gtgcggttat ccggacgcgc ggtaaaaaag aatcaacaga
   112021 atttgtaaca aaggtggcag caaaagttca tggaagaaaa aattaatctg gtcgcggcaa
   112081 tgatcgcggc atcgaaaaaa accgtcattt ttaccggagc gggcatcagc acggaatcgg
   112141 gtattccgga tttcagaggg cccgacggtc tctggacgaa ggttgatccc gaagatttca
   112201 cgatcgaccg gtacttgcgt tccggcgaga cgcgcaaaaa ggtctggtac tacctggtgg
   112261 aaggcggatt gatgaccacg tcccaaccca acagggcgca tctggcgatt gccgaattgg
   112321 aaaggatgaa caagttaagt tcaatcatta cgcagaatat tgacaatctg caccagcggg
   112381 cggggaatga cccgaaaaag gttcatgaac tgcacggcaa tatgcagtgg ctggtctgcc
   112441 tggactgcgc tgaacgctac gatctggaaa tgatgaggca gaagcatccc tcaccggatc
   112501 attttccgat ctgcgaaaaa tgtgcggggt tgctcaaacc gggagttgtt tttttcggtg
   112561 aaatgctgcc cccggatacg ttgaggatgg ccgagcagga atccgaaaaa tgcgatttgt
   112621 tcatggtgat cggctcttcc ctggtggttt atccggcggc gtacatgccg ctgtatgcca
   112681 aacaatccgg ggcgaagatc gtcattgtga atatgggaca gacgggacag aacgatatcg
   112741 ccgacgtatt catcaatgca ccggcgggag atacgctgag cagaatcgtc gcccgattaa
   112801 aagaaatcat gacatgactt ccggatggta tgaaacccca tgtgtctgat cgtttttgcc
   112861 tataatgtgc atccgtcata caggctggtt ctggccgcca accgcgatga attttatgaa
   112921 aggccgtcat cgccggcggg cttctggaaa gatcatccgc ggatgctggc cggccgcgat
   112981 ctgaaaagcg gcggaacgtg gctgggcata acgaaagacg ggcgattcgc tgccgtcacg
   113041 aattatcgcg acccgtcctc cttaaaaagc aatgcactgt cacgcggcct gcttgtcagc
   113101 agctacctca acgggaataa agatccggga aactacctga aaaagattgc ctcggatatt
   113161 tttaactaca acgggtttaa tctgcttctt ggcgataaac gggatgtatt tgttttttcc
   113221 agtcgcggaa aagcgcaaaa gctgaaaccc ggcatctatg ggttaagcaa tcatcttttg
   113281 aattcgccct ggccgaaagt atccagaagc aaacggatgc tcaagaccgc gctgatgcag
   113341 aaaggcgatg ctctggaaga cgcattgttt tccatgctgg ccgaccgtcg gattcctccg
   113401 gatcaaaaac ttccctccac gggaatcggt ctggaatggg aaagactgtt gtcctccatt
   113461 tttattgaaa gtccggttta cggtacaagg tcgtcgacgg tcttgctgat cgggaaaaac
   113521 agacatatcc ggtttgcgga gaaggttttt gacgggcaga aggatccctg gatggaaagc
   113581 cgtttttcat ttatcgtaaa actaaagtaa aaaatggcgg atgctccggg cagcccctta
   113641 tccgcaaatg cgttgtgtcg gcttcaaggc catgacggcc agggatgatc gtttgataga
   113701 agcgaaccac ggcccgtccg ctgatgacaa atcttcggaa actatcagcg tgtgatctct
   113761 agccgaaaat acaataaatg tttccaatag tttaggaaga aatgatgcaa caggccggga
   113821 aaagaggatg tgatgaaagt tgtttaccat gaagattaca acaaagttta ttcttccgat
   113881 cccgcttccg cgctgggaag aattcaggcg gtggaaaaaa tcctgtgcgg caaagtgacg
   113941 tttgtcgaac cggcggcggc atcccgtgaa gacattctca gcgtgcatac acccgatcat
   114001 gtcagggccg tcgagcgcga aggcgtttac gatattgccg cgctggccgc cggcggcgcg
   114061 atcaaggcgg cgcagatcgg gttgacggaa ccctgttttg cgctcattcg tcctcccggt
   114121 catcacgcgt cttccggctg cgcctgggga ttttgttatt ttaataacat gtcgatagcg
   114181 ctggaaaaac tcaaacggga aggtcaaatc agaaaagcgt ttattctgga tttcgatctg
   114241 catttcggtg acggaaatgt gaatattctg ggaggtaagg attatgtgac gattcttaat
   114301 ccgacagggt ccaatcgtaa tgactattta aaacaggttc aaaacggtct tgccgaaacg
   114361 gatgccgata tgattgccgt gtccgccggt ttcgaccatc acgaggacga ctggggcgga
   114421 cttctgaaga ccgaagacta ccaatacatg ggcggactgg tgagagaggc cgcccggcgg
   114481 aacaacggcg gatgcttcgg tattctggaa ggcggctaca atcacactgt cctggggaaa
   114541 aatgttctgg cttttgtcga aggcctggcg gaaaaagatt tgtaatcggc attgtgaaaa
   114601 tgaaattgca atagggtctt gccgatactg aaatgcaaag gaacaagcgg catcgttttt
   114661 ttgtccggga tcagacggtt aagagataaa caaatcatta aaagacggaa tgatggaggt
   114721 gtccgaacat ggccggtttt gctgaatatg atcaatatga cggattaggt ctggctgcgc
   114781 tcgtccggga gaaaaaagta acgccgaggg agctttgcga agaagcgatc cgttgcattg
   114841 agaaattgaa ccctgaattg aatgccgtgg tcacaccgat gttcgatttc ggacgcgatg
   114901 ccgcagaaaa aaatacggca gatggtccgt ttgccggggt tccgtttctg ctgaaggatt
   114961 tgatcagcgc ctttgccggt gttcggttaa cttggggctg ccgcgcctat aaagattata
   115021 tcccggatta tgacagcgaa ctggtcaaac gtttcaaaaa ggcgggactg attacgctgg
   115081 gaaaaaccaa tacgcccgaa ttcggtttga tgggcattac cgagccggag ctgttcggcc
   115141 cgacgcgcaa tccgtggaat acggagagaa cgagcggcgg ttccagcggc ggatcggctg
   115201 cggcagtggc ctcgggtatg gtgccgatcg cctcaggcgg cgatggaggc ggttccattc
   115261 gcattccggc ctgttactgc gggctggtgg gattgaagcc gaccagaggc cggactccca
   115321 cggggccgaa atacggaaga ctctggcagg gcgcagtggt ggaacacgtt ttgacgcgct
   115381 ctgtgcgcga cagtgccgcg atgcttgatg ccacgctcgg cgacgagccg ggatcgcctt
   115441 atgatatcaa accgccggaa agaccctaca gcgaagaaat aaaccgggcc cccggttcat
   115501 tgaaaatcgc cttcaatacg gaatctccgc tgggcaccgg cacgcacgaa tcctgcgccg
   115561 atgccgtgat caaaaccgcc aagctgctgg agaatctggg acataacgtg gaagaggcca
   115621 aaccggatat tgacggtgtc aaactggctc atacctattt catcctttat tttggagaaa
   115681 atgccgcgga tattaaatgg gctgaagctg tattgggaaa gaaagtcggc cgaaaggatt
   115741 ttgaaatcac cacgtggttt ttcggtgaac taggccgctg ttacagcgcg ggggattttg
   115801 tggcggccat ccgggaatgg gatgtcgctg ccagagtcat gggacaattc catctcgact
   115861 acgatttata tctgacgccg accgtggcct atccggcagc aaaaatcgga gaacttatgc
   115921 cgaagtccta cgaagaagca gccatgaaaa tattcagcgt cctgaaactc ggttctctcg
   115981 cgaaagtgtc cgggatggtg aatcaaatcg ccatcaagac gcttgccaag actcctttta
   116041 cacaactggc taattttaca ggtcagccgg cgatgaatat tcctctgcac tgggatgaac
   116101 agggtatgcc ctgcggcgtg cagtttgttg ctcctttcgg cgacgaagcc acgctctttc
   116161 ggctggcggc tcaactggag aaagccaacc cgtggtttaa cagacgacca tccctcaaac
   116221 tctgagtaat ttcatcggcc ggcgctcagg caaaaacgac tggtattttc cggaacaatc
   116281 gtatagaaaa tctattgttg taaatttcac atcagccctt tgcggacgtt ttgcgtttta
   116341 aatcaagggc aacaggagta gatcatgctt tcagaaaaaa ataattcccg gttcggcggc
   116401 gcatccaagt atattctgga cgatgaactg gcaaaaattg tcaatgtttc catggcgctg
   116461 gaaatgcccc tcttgctcaa gggcgaaccg ggtacgggga aaaccatgct ggctcacgcc
   116521 attgccgaga gtctgcagat gccgctgatc gttttgaacg tcaagtcgag catgaaatta
   116581 attgaggcgc tttatcagta cgacacactg acgcgtctga acgacagccg cttcggcgat
   116641 tccaaacgcg atgtcagcga tattgaagcc tatataaaaa tgggcaagat cggccaggcc
   116701 ttcaccgccg acaaaaaaac cgttttactg attgatgaaa tcgacaaggc cgacaccgat
   116761 tttcaggacg atatgctgga cgttctggat cagatgcaat tcgatattat tgaaatcgac
   116821 aaaaccatcc gggccaagaa ccgtcccgtg atcgtcatta catccaatgc caagaaggat
   116881 ctttccgatc cgttcctcgg acgctgcaat tttcatcaca ttgcgtttcc cgatcccgat
   116941 atgatgcgcc tgattgtttc cgtgcacttc cccaaactcg atgaagagct gatgaaaatc
   117001 tgcatcagca cgttttatcg tttgcgggag ctgcggggcg ttgagaaaaa accggcgacg
   117061 cgggagctca tcaactggat tcgcgctttg aagagcgatc cggatttcaa acccaaaacg
   117121 ctttatcagg gccgggtgcc tttcctggga gttcttttca aaaagagcgc cgatctacag
   117181 caggccgccc agcaggttat caaatcgcgc ggttgaggtg taaacggtgt tcgttaattt
   117241 cttttatacc ctgaaggacc gcggtattcc ggttacgccg acgtcgtttc tgcgcctgca
   117301 gaatgccatg agcaaggggc tggttcattc cctcgacgat ttttacaccg ttgcgcgaac
   117361 gattctcgtg aagagtgaac gctattttga cacgtatgat cagatattcg cccaccactt
   117421 cagaaatgct tctctggaag atcccacggc ggtggaatta aacgaagtag cccgagcgat
   117481 gctggaggaa tggctgcaga atccgcagga actggccgat atgctcggca tcgacaagga
   117541 caaactgaaa aaaatgacgc cggaagaact cattcagtat tttctcgagc gtctgaagga
   117601 acagaccgaa gcgcatcacg gcggcaataa atggatcggc accggcggaa catcgccgac
   117661 aggacattcc ggctatcatc cgggaggcat gcgcgtcggc ggcgagtcca gaaatcattc
   117721 ggccatcaaa gtggcgctgg accgccgata ccgcgattat tcccaggaag gaccgctgac
   117781 ccagtctcag atcggcgaag cgctcagaag gctgcgtcgc ctgatgccga cgggaccgaa
   117841 ggatgtcgtg aatgtcgaca aaacgattta cgaaaccatg cgcaatgccg gggaaataga
   117901 catcatcttt gaccggcgtc tgaccgaccg cctgaaggtc atgctcttta tcgataacgg
   117961 cggctggtcc atggaaccct acatcggagt ggttcaaacc ctctttcact acgcgagctc
   118021 gcagttcaag gatttgaaga ttttcttttt tcataacacc atctacagcc gcgtctgggc
   118081 cgatccgcag cgctattata aaccgcagga cgtggacgaa ctcatccgct gggatcctga
   118141 gacacgcgtg atcattgtag gggatgccag tatggctccg tacgaactca tgcatcccaa
   118201 cggcgccatt tatgttgacg ccaaaccgtc gggaaccagt attgagcggc tgcgcttcat
   118261 cgcga
//
LOCUS       DAONQZ010000002        91192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_234_length_91192_cov_13.367831, whole genome shotgun sequence.
ACCESSION   DAONQZ010000002 DAONQZ010000000
VERSION     DAONQZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 91192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 91192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91192
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234_length_91192_cov_13.367831"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..285
                     /locus_tag="PLR13_00570"
     CDS             22..285
                     /locus_tag="PLR13_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17849.1"
                     /translation="MQVNLTEQEIVKFAYVTKEIIDQSWGATLSNAQINCYIECFYTL
                     KDLLEQIDREYLQKHSEISDYFLTGKRLINEKDKLQWEKEWQV"
     gene            404..>1730
                     /locus_tag="PLR13_00575"
     CDS             404..>1730
                     /locus_tag="PLR13_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC17850.1"
                     /translation="MKIVLLSMPDSIPMIMHESAFHLPNCGIASIGANLDEGHDVYVV
                     DLIRKRRHLAGYLKRLLLKIHPKLVGLSAMAWQYETCIEIIRFIRLLLPEVKIAIGGY
                     HATLMYEEIAASPESGLIDFMIRGEGEEAFRRLVNALEGSDQLQDIPSLSFKRRGTFC
                     HNPRGALLDLSRLKIPIRDKRRLTSGYHAVFSKIEVIETSRGCTRTCSFCSMREMYGQ
                     TFRTFPIERVTADIDDIYYKHNTRAIFIVDDNLVLDPERVIDLCDAIIAKNYKGLSLH
                     VQADCISIAGNETMVRKMSLAGVKTVFLGIENVVKKNLMASGKGDIVDLSRKAVRICH
                     QFGIMVIAGLIFGFPDDDENDIVDNYRFLKSLDADIPYCQILTPYPKTPVRQQLLERG
                     MVTNLHDYKLYNGLRANIKTRHLDTQQLQYLVWLHRQKMFGWWEPSRLMR"
     assembly_gap    1731..1830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1831..2376
                     /locus_tag="PLR13_00580"
     CDS             <1831..2376
                     /locus_tag="PLR13_00580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQC17851.1"
                     /translation="IRGGSIAAGYGVTKSYVDILTQALLPEGVCVINRARYRETSFDG
                     IETYPQDIDRYEPDFLLIHFGVDDAFQCVYRSEFQENIVQMIRLSRKRLQPVIFLATS
                     HTFDNPNDMDAVHIFYRSLRIVASDLKCEFIPVHRSWNAYLEEHNLCSQDLTQEDSRY
                     PNEQGHQVIANAMMNVLGKFF"
     gene            complement(2406..3788)
                     /locus_tag="PLR13_00585"
     CDS             complement(2406..3788)
                     /locus_tag="PLR13_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQC17852.1"
                     /translation="MEKFFDPHSVVVVGASNTPFNLGATICNMLKDYLQYPGAVYAVN
                     SKGEKVNGYPGYSSILDLPESPDLAVIIVAAKNVPRIIGDCARKGIKRVIIESAGFSE
                     GGSDGKAMQRKIDEIAKAHGIRIMGPNCLGTLDTRKKFCCFYGVNPSLVEMGRIFEAP
                     GNISFIIQSGGVVVLVIESFFYDLVGVNKVVSIGNKCDVDEADLIDYFQTDETQVIGL
                     YLESVTNGRKLMEAARKSHKPILIYKAGRTQEGALAAMSHTAGMANNDSIFNSACKQS
                     GMIRLQSIDELHSMPKMLTEMPLLKGKNIAMFTNSGAFGSIGADLLVDAHLQVVRLKP
                     ETQEKLKKAGQVFNIKNPVDIGPALPQTYLDIYEILLSADEVDGLLPLLSVWQTFVID
                     TLLELMKMCRHYQKPAAIYTPNAIAKSIGIRAKHRIPIFETAEQAVRALAVSGYHYDS
                     LQKRFPARAK"
     gene            3956..5749
                     /gene="mutL"
                     /locus_tag="PLR13_00590"
     CDS             3956..5749
                     /gene="mutL"
                     /locus_tag="PLR13_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQC17853.1"
                     /translation="MSKRIIVLSEDIANKIAAGEVVERPASIVKELLENAIDAGATDI
                     RVELEKGGTQSIRVTDNGSGIDHDDVPLVFERHATSKIHQFEDIFNVVSFGFRGEAMP
                     SIASVAKVEILTRRKNDVSGTRAILDGGVIRAMDPAGMPEGTQVSVSGIFAHVPARRK
                     FLKTEATEQGACLDVITRLALAHPEIRFKVEANGKEIFAAPEVHDTAKRIAMVMGGEF
                     SAHCMAVDAQQDAMTLKGFISRPEYSKSNSKSIYLFVNKRFIRDTSLTHAVLSAYRQI
                     IESRRYPAATLFLEVPPEDVDVNVHPAKMEVRFKNAHGVYELVSKTIAHHLSRAETSR
                     GHYLYRLAPAEKKDYFHRQPFVNEPSPSMPSRPADRSHAQQGIQNILLHRSQVSQPPA
                     ASEGDAGVPDKTILFGDMRYVGQYDGTYLLFEGPDSLILLDQHAAHERIILEKLKKDS
                     GKKAAGQSLLLPEIVNLTPGQMALLNDALDFLETIGLEIECFGRDAVAVKTVPTMLSK
                     VKISEMIYDIVDQLADQNRTPSLQERSDNLLASLACKAAIKANRLLSRDEVDALCREL
                     AETPFHATCPHGRPITIQFSLSEIERLFKRK"
     gene            5761..6696
                     /gene="miaA"
                     /locus_tag="PLR13_00595"
     CDS             5761..6696
                     /gene="miaA"
                     /locus_tag="PLR13_00595"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQC17854.1"
                     /translation="MKKLPLVIINSPTATGKTDLAVYLALECGGEIISADSMQVYRYL
                     DIGTAKPTMEQRRGIRHHLIDVVNPDGEFNAALFAARARAVIEELVHQGKPVFVVGGT
                     GLYIRALLKGIIDTPDVDASIRNHYRSLRETHGKIYLYDLLRQKDSAAAAQINPNDSV
                     RVIRALEVLEQTGESITVKQKKHSFSEDRYTTCKIGLRVERGELKERIEKRTDAMIRA
                     GLLDEVKGVLARGYRENLKSLQSLGYKQIIGFLNWQYDWRHCVNFIKRDTWQYARRQM
                     TWFSADKEINWFAPDALDILRRHVKIFQNENQETE"
     gene            6693..7334
                     /locus_tag="PLR13_00600"
     CDS             6693..7334
                     /locus_tag="PLR13_00600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HQC17855.1"
                     /translation="MTRRDHHTSVEYLCRLQRLFVVVLIALPLNIGAATHYHCADRSG
                     LQTFSDYPLEDQVCTEMVSQEETEIPKTDRPIAPNAESPEKKTTRIAVKENGILVPVT
                     LGYDGREADIRLVLDTGASATTIYDNVAGRLLVNLNNAPKAQGRVVGGGTIDANRVTF
                     SYLRIGPNTFRDWNIFVVENKDGDTSYDGLLGMDVLSTMRYKIDFRNQIIIWE"
     gene            7513..8760
                     /locus_tag="PLR13_00605"
     CDS             7513..8760
                     /locus_tag="PLR13_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQC17856.1"
                     /translation="MMNRKRDRIIFFTLLIFASLLTLAGCEPKVRVAVSQLDTPGHHT
                     YAGLKLLELEKYSDAIRAFKMATQLDSNYSKAYTGMALVGIATGKLSAAADDLEKGIL
                     NAKTSDEELFVNTAAIRYHTAKKSDEKWLEKAKYYFVETVMIDSQYAPVYYFMGIAYQ
                     EALEFDQAAKMFNKVIQLKAGHIEDANNRLEFLELVHKAHPATQAGKQIALKETVSRA
                     DIAMMLAEELKIKELYQKYLPKTAQEAQTEEEKVAASMSQAEHVAKEMEKMDQFHSYM
                     VLQEKEPVKLLANDIALHPLKNCIEAVLEAKVHGLENDPKGNFKPDEILSRGEFAIIL
                     EDILIKVSGERDIAARYVSSNSLFPDVPADMPYFTPIISVTSRGIMEAKNTKTGEFSP
                     LKPMSGVEALLAVKKLKENLKID"
     gene            8845..11502
                     /locus_tag="PLR13_00610"
     CDS             8845..11502
                     /locus_tag="PLR13_00610"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQC17857.1"
                     /translation="MAKRELGKAFEPAAAEKRLYEYWLKNKYFHAQDKSEAEAFSIVI
                     PPPNVTGMLHMGHALNNTLQDIIIRFKRMQGFNTLWMPGTDHAGIATQNVVEQQLLKE
                     GTSRHDLGREKFIERVWEWRRKYGGVIINQLKRLGCSCDWERERFTMDEGLSHAVREV
                     FVRLFNEGMIYQGDYIVNWCPRCHTAISDLEVEFKSDASFLWNIRYPFAEGQGEIVVA
                     TTRPETMLGDTAVAVHPDDKRYKNLVGKYVILPLMNKKIPVIADDYVTMDFGSGAVKI
                     TPASDPNDFAMAQRHHLEIISVIDGKGIINENGGAYKGQDRYEARKNIVNDLQKGGYL
                     VGTEPYAHNVGQCYRCKTDIEPMVSKQWFVKIKPLAKQATAAVIKGKTKIVPKTWEAT
                     YFDWMNNIRDWCISRQLWWGHRIPVWYCDNCKKVIVSTVDPDTCPDCGGASLKQDEDV
                     LDTWFSSALWPFSTLGWPEKTAPLKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDV
                     PFKHVYLHALVRDEKGEKMSKSKGNIIDPLLMIDQYGTDAFRFTLAAYTAQGRDVRLS
                     PERIEGYKFFVNKIWNASKLTFANLDDFDETATAEVADSLADLWIKARLNKAVEDVTT
                     ALDEYRFNDAAAAVYRFIWHELCDWYLELSKPVFYGKGSAEQKQAAQKTLGVVFPTML
                     KLLHPFMPFLTEELWQVFHGDDKNSIMISRFPAVCSEWKNDAAEKEMETIMDMITAVR
                     NIRGEMRIAPSQKLDVLMSTVDEKTQKMVEKHKDYILNLANLESLTVEINAVEPKGVA
                     TAVVGSTKLFVPIVGIVDLSGEIARLEKELAKISKDLQQSSKKLANRDFMSKAAPDII
                     QKEEDKVKDFQKKHTTLENALKKLKGMSA"
     gene            11502..12338
                     /gene="nadC"
                     /locus_tag="PLR13_00615"
     CDS             11502..12338
                     /gene="nadC"
                     /locus_tag="PLR13_00615"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQC17858.1"
                     /translation="MEQRIVRIIQNALAEDIGSGDITTGATVNARKKGRAVAIAKGNF
                     IVAGTDVFAGTFRTLDKNIKVQKRIPEGYPVKKGDIIAEVSGRLDHILMAERVALNLF
                     QRMCGIATLTHRYVRAVRKTKAGILDTRKTVPGLRVLDKMAVKTGGGQNHRFGLYDGV
                     LIKDNHIEAAGGITAAVKAQRKKLSRFMKIEVETRNLDEVREALECGADIIMLDNMSV
                     SAMKRAVSFVAGRALLEASGNVGLKNVMDIAATGVDFISVGELTHSVQAADISLKIRS
                     SS"
     gene            12356..13168
                     /locus_tag="PLR13_00620"
     CDS             12356..13168
                     /locus_tag="PLR13_00620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQC17859.1"
                     /translation="MDWNIARLQETLGTGLFGGHLHFYPEIVSTNDEAIALAMAGAPE
                     GTAVLADAQTAGRGRWRRSWISPPGVNIYTTIVLRPECESSGASQIPIMAGVAAAEVL
                     DGVCPGRVKLKWPNDVIIGPKKVCGILSQAKITSKKIDFAVVGIGINVNAGVHHFPKT
                     IRESATSLLIESGGEISRHDLLISLYENLEKWYKQLLQKGFEPVRKKWLESAAMMGRP
                     IRIAFKGESTEGIAKGLGDDGCLILVVEGNEEIKVSAGDATIMRWKENAFGD"
     gene            13152..13928
                     /locus_tag="PLR13_00625"
     CDS             13152..13928
                     /locus_tag="PLR13_00625"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQC17860.1"
                     /translation="MLLVIDVGNTNTVLGLFDGKNLIHDWRIRTEIDHTIDEYGVLIY
                     NLYQSSRKKEKPFKTVSAIIISCVVPPMLNILEPLCIKYFNVKPLIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAYNKYRKESIIVDFGTATTFDYVSPKGEYMGGCIAPGIVIS
                     SEALFEKASKLPRVEFSRPKSAITKDTVSAIQAGIMFGYAGLVDGIVNRIRTEVETKP
                     LVIATGGLANIIAPETKTIEKIEEMLTLDGLRFIYELNKK"
     gene            13931..14548
                     /gene="coaE"
                     /locus_tag="PLR13_00630"
     CDS             13931..14548
                     /gene="coaE"
                     /locus_tag="PLR13_00630"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQC17861.1"
                     /translation="MLNVGLTGGIACGKSTVAKMFVEKGAHLIDFDKLAHRLQEPGQP
                     AWQKVVDYFGRQILRPDQKIDRVKLGRIVFADKKKLRVLNKLVHPPVYREWQLRLEKI
                     GRKEKHAIVLSDVPLLFEGKKTHLFDLTILVLSDPEDQICRLISRNRISGDDARARLK
                     SQMPIGKKTALADIVIDNRDSIARTKKTVDRVWRELVRLEKTKSA"
     gene            14594..15211
                     /locus_tag="PLR13_00635"
     CDS             14594..15211
                     /locus_tag="PLR13_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQC17862.1"
                     /translation="MIEKNVKTDFSSKISEPVIGAGTYVHPLAAVIGNVILGENIMVA
                     PTACIRGDEGQPLHVGDDSNVQDGVIIHALETELDGKPVEKNQRDVDGKKYAVYVGKR
                     VSLAHQVQIHGPAVVMDDTFVGMKTLIFKSFVGKHCVIEPGVTLMGVTVDDGRYVPAG
                     SVVKTQADADVLPVITPDYPMKEMNKGVLHVNKALAKGYLAAAKK"
     gene            15355..15981
                     /locus_tag="PLR13_00640"
     CDS             15355..15981
                     /locus_tag="PLR13_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQC17863.1"
                     /translation="MKCRQNLRQKLNLKNGLLQALNTKYATFYFSKTMIILDVVPYGS
                     REIFRYAIRRRAGNTPAIDFSFMTNNLGKAWWAVSDTTHRNYLASQHVLKMAKRYTIT
                     DKSQRPKIVKIPAVKVSALNCLDIKKIDLASCDLDRVIKSAYRRQVKIHHPDLGGKAA
                     DFRKIHSAYKELLAWADHPVFVHRRGFPDKWFYSAETQKWVQPTPLSD"
     gene            complement(15992..16576)
                     /locus_tag="PLR13_00645"
     CDS             complement(15992..16576)
                     /locus_tag="PLR13_00645"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQC17864.1"
                     /translation="MQENVTGLILAGGKNSRMGVNKAFLEIDGKRLMDRTLSVYRRIF
                     PKIIIVTNDPLAYVEFSEAMIVTDIYKGKGSLGGIYTGLFYAASDDCFVAACDMPCLN
                     EDFIRYMIGRRRSYDIIVPELSEGFQALHAIYSRRCLAVMKKMILADRLKIAGLFKDM
                     HTLRLTEEDIRPFAGNTPLFTNINTPQDLQSFRK"
     gene            complement(16588..20856)
                     /locus_tag="PLR13_00650"
     CDS             complement(16588..20856)
                     /locus_tag="PLR13_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HQC17865.1"
                     /translation="MQTLGINIGSSSIKLILLEDDRIVWQKVEPHEGNFVHALEKTLS
                     ERSFPPGVLTLATGTEGRNLLNVNSVIEPLCIEEALRHLNGNVNALVSLGGEDLVVYS
                     IDENQQIITSFSGNKCASGTGEFFKQQLGRMNMHLNDIHSIPEESRILKLSSRCSVFM
                     KSDCTHRLNKGEASTGDIVLSLSDVMATKVIDFIKKAKIAGKKVLLVGGVTRNPHLIR
                     FIRQRMPENDFIVPDQAVYFEAYGAALLAGSMGTPLPALNDLFKTHMVQFKRFKSLKT
                     AEGKVHYLPSKKSKVIAGREYILGVDGGSTTTKACLIDMETSEITASFYGRTHGDPVK
                     ALKNCFLEMQKQIREDIGDRAIKITLAATTGSSRELLGVFLETPGVYNEIIAHAVGTT
                     FYQEDIDTIFEIGGQDAKYVYLKNKVPIDYAMNEACSAGTGSFLEESAQGDLNIHHAW
                     EIGDIALGASEPLKFGEHCSAFINSDVRKAIQQGASREDITAGIVTSIVSNYLNRVVG
                     NRTVGQNIVLQGGVAKNKAVPLAFAMLLDKNILVPPDPELMGCFGVGILARQKLHEGF
                     LSRRDYDIEKILSTEILHEKEFQCSACDNFCPIRVLNVNGRKYMFGGRCNKYANIRKK
                     KSFDESDVVDYIEQRNDILFNQCAPRPADLIRKKDFVVGIPKCFSVYSHWPLYAWFFH
                     TLGVETVLSSDITHEGVARVESTYCFPAEIAHGAVQDIFNRNVDYIFLPHFRDMESYE
                     SNTPACFCPITQSLPYYIKKAFPEIPEGKYLAPVVSFMYGTQKASESFIAMAVRLGFD
                     QEDARLAFSVALEKQMECFRKFAELGKIAIENSRKSQRPVIAVLGRPYNAFTPDANMG
                     IPRKYTSRGFTVVPFDILPFEDQGIYPNMYWYYGQQDMKAAALLKNEDNIFVTYISNF
                     SCAPDSFMLHYLKWIMGMKPFLVLELDSHTADAGVDTRIEAFLDIIEGYRSKISDIRE
                     SRYDNGLRFINTSGEDIHIRNTLTGEKIPIKNNPRVKLLLSNMGRLSVEVLAATIRGL
                     GIHAETMPVPDIYTLQMARNHASGKECLPSHLVLGSTLKYLSSEKYRKDEIYILFVPT
                     TTGPCRTGQYFVFYENLFKDLRLENVVVFTLDADNSYNELGTEFSKYAWWAVLIGDYM
                     KDIETSLRTCAADPVKAVSDYNIIWQKLIDVAEKDVTQSLPVLKEISSSIAKIPLKKK
                     IDDCPKVLIVGEIYVRRDDFAVDELIRHFSRRGIIGKVSNVAEWVYYCDFVRRHELQK
                     RLKLVPWYKKFLAAEFSDIVKWKIEHVYKKKVEKNIRDILSTTGLVPHAPHNMDEIME
                     NTEKHFVNNELYSEISISSGVAATAMMDGYAGIVNISPFACLIGRVIEGVLTPWARER
                     KYPAISIEIDGNLFPPNVISKLEIFMLNVMRFRNQDETDHRTTL"
     gene            complement(21090..21761)
                     /locus_tag="PLR13_00655"
     CDS             complement(21090..21761)
                     /locus_tag="PLR13_00655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQC17866.1"
                     /translation="MEPLIFFHTQWLKDVGIDMTIVFNTLFVMALLAFFSFLATRRTE
                     VYPGKTQNVMEVIIGGFDGLLNEIMGHNGRKFFALISTLGLFILVSNLIGIVPGFESP
                     TSNINTNAAMALIVFLSTHVVGIKEHGLKYFKQFMGPVWWLTPLMLPIEIISHISRPL
                     SLTFRLFGNIKGEDIVLLVVLFLVPWFVPLPVFVLMVFTSVVQTLVFMLLAMMYIAGA
                     MEEGH"
     gene            complement(21768..22163)
                     /locus_tag="PLR13_00660"
     CDS             complement(21768..22163)
                     /locus_tag="PLR13_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQC17867.1"
                     /translation="MNLIVKDPLQKRIEITNWIILAVIFIPSLFLAPLKFSLGILLGG
                     FVSIVNFHWMARGLRGIFQNLQGNPKGTVMVKYYIRLAIMAVVLYLLIANNVVDIVGL
                     LIGLSVVIINILVTMIIALAKKNFIEEVV"
     gene            complement(22123..22407)
                     /locus_tag="PLR13_00665"
     CDS             complement(22123..22407)
                     /locus_tag="PLR13_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQC17868.1"
                     /translation="MDKETRKYAMQMAFASSIGIAMILAVFGCLLLGSYLDKKFDSGH
                     VFTVVFLFIGLAAGFRNLYVLVQKNFTDEETIIKSLKSEPHRQRPAPKKD"
     gene            complement(22467..23411)
                     /locus_tag="PLR13_00670"
     CDS             complement(22467..23411)
                     /locus_tag="PLR13_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQC17869.1"
                     /translation="MLPQKRITITGARGFLGTHLLKKFKSKGCRNIEIADLPEYNLTD
                     INAIRRMYEETKPDVVIHLAAKVGGIGFNQEKPAELFYDNLIMGAQLLHEAHIRKIEK
                     FVALGTVCAYPKFTPTPFREEDIWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGFNSV
                     FLLPVNLYGPGDNFDSRSSHVIPALIKKCFDAIDRQSDQLEVWGTGSATREFFYVEDA
                     AEAIYLATLLYDKSDPVNLGSGFEISIKDLTTLIAELTGFSGRIVWDSSKPDGQPKRM
                     LDTSKALKEFGFIAKTDIRTGLTKTIDWYKKNRHLLKK"
     gene            complement(23422..24453)
                     /gene="gmd"
                     /locus_tag="PLR13_00675"
     CDS             complement(23422..24453)
                     /gene="gmd"
                     /locus_tag="PLR13_00675"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQC17870.1"
                     /translation="MKKAFLTGITGQDGSYLAEFLLHKGYEVHGLIRRSSTFNTERID
                     HLYHDFHDPEARLFLHYGDLSVSAQLMDLLHSVKPDEIYNLGAQSHVRVSFDMPEYTG
                     DVTALGTLRILEAIRKTGITTKFYQASSSEMFGAAPPPQHEKTVFQPRSPYAAAKVYS
                     YYITQNYRDAYGIFAANGILFNHESPRRGETFVTRKITRAATQIKLGLKDKLYLGNLE
                     AKRDWGFAGDYVEAMWLMLQQNTPDDFVIATGETHSVREFAEMVFQKLDLDYKRYVEI
                     DKRYFRPTEVDALLGDSTKAKKILNWQPRVSFENLVDMMIASDMELAQKEKTLLDAGF
                     VSNNQNFNH"
     gene            complement(24527..25810)
                     /gene="hemL"
                     /locus_tag="PLR13_00680"
     CDS             complement(24527..25810)
                     /gene="hemL"
                     /locus_tag="PLR13_00680"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQC17871.1"
                     /translation="MKKTTSVQFSKANRVIAGGVNSPVRAWKSVGGHPRFVLKAKGSR
                     IYDTLGKAYIDYVLSWGPMILGHAHPDVTAAVRAALKQGTSFGAPTLLETAIAEMISS
                     ALPSIERIRMTSSGTEAAMTAIRLARGYTGRKLIVKFDGCYHGHSDSVLIKAGSGPTT
                     LGIPGSPGVPDDIARLTLSLPYNDTNALAKTVKRYGSQLAAVIVEPVAGNMGVVAPRE
                     EFLKQLRVLTAKNGIVLIYDEVITGFRFTFGGYQNLVGITPDLTCLGKIIGGGLPVGA
                     IGGRKKIMERLAPTGDVYQAGTLSGNPVAMIAGLTTLQLLKAKKKSFASLDKKTDQLC
                     RAMHEMFFKKGIPVCINRVFSMFTVFFQEGPVVDLQTALQSDTNMYARFFHGMLKNGI
                     WLAPSQYEAGFLSFAHTEKDLQKTLQACAKTLRNL"
     gene            complement(25810..26277)
                     /locus_tag="PLR13_00685"
     CDS             complement(25810..26277)
                     /locus_tag="PLR13_00685"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17872.1"
                     /translation="MRKLTSKQKKIARLLQKDIPVTKFPFKKLSGAPTPGAKEILTAL
                     GQFREQGLIRKFGAILRHQKAGYGQNALVLWSVPPSQTETTGRLFASFSFISHCYERH
                     PAFLERYNLFTMLHSDGRSISSLIKDMVNATGIGDYLILKSLKEYKKTSPEYF"
     gene            26475..26852
                     /gene="atpE"
                     /locus_tag="PLR13_00690"
     CDS             26475..26852
                     /gene="atpE"
                     /locus_tag="PLR13_00690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQC17873.1"
                     /translation="MKKSLMYVMLAMVMVIVSAPFVFAAPEAAAQAPAGTVDYTKAIV
                     ASIFALAAGLAIGLGAIGAGLGLGQATSGASNAVGRNPEAQGKVMLTMMVGMAMTESV
                     AIYALVISLVILFANPLLKIIGM"
     assembly_gap    26919..27018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27022..27429)
                     /locus_tag="PLR13_00695"
     CDS             complement(27022..27429)
                     /locus_tag="PLR13_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HQC17874.1"
                     /translation="MPVSAEKEKMLQDRMKRLGVSETDLEETFIRSSGPGGQKVNKSS
                     SCVYLRHLPTGLSVKCQKERSQSLNRFLARRLLLDKIEIQQKGLMAEARQTIEKIRRQ
                     KRKRSKRAKEKILTDKHQQAEKKILRAKVSVEE"
     gene            complement(27451..27882)
                     /locus_tag="PLR13_00700"
     CDS             complement(27451..27882)
                     /locus_tag="PLR13_00700"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17875.1"
                     /translation="MPVKQISVILNNVPGSFAALTHILDLEDISTKAVSAASIADDSK
                     VRLVVNDPDRAAAVLKSYNLNFEVTPVLAVEVPLHAGGMNAILKPLAKAEINIHYLYT
                     TINRFGEETIVILGVNKIDEAREILEQNWIHLIDEEIYSIP"
     gene            complement(27893..28435)
                     /locus_tag="PLR13_00705"
     CDS             complement(27893..28435)
                     /locus_tag="PLR13_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQC17876.1"
                     /translation="MLIKTIFSGFGGQGVLMMGVSFAHSGMSQGYQVTYLPAYGAEMR
                     GGTANCTVAIADEDIASPVASEPDFLVAMNSPSLFTFQNKISSGGTIFLNSSIIKEKP
                     HRSDIKVCSMPCADMAQDLGNIRVSNTIMMGAFIKVSAVIPPDVYLKSLETIMGSKKK
                     SLAELNRKAFNAGYDYVKND"
     gene            complement(28435..29205)
                     /locus_tag="PLR13_00710"
     CDS             complement(28435..29205)
                     /locus_tag="PLR13_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQC17877.1"
                     /translation="MVKTVFKRPRSLKENVFHYCPGCGHSIAHRIIAEVIDELDIRGI
                     TIGVPPAGCAVLAYNYFDVDMIEALHGRGPAMASAIKRSLPDRVVFSYQGDGDLAAIG
                     IAESFHAANRGENITIIFINNAVYGMTGGQMAPTTMLEQKTTTSPAGRHRTLDGYPVK
                     VSEIFSQLEGVTYLERCMVNSPLNINKTKKAIKKAFQCQLDGLGFSLIEILSPCPTNW
                     KMTAFNSCKWIDEVMSKTFPPGVYKDNIAAAKKSAEGR"
     gene            complement(29226..30284)
                     /gene="vorB"
                     /locus_tag="PLR13_00715"
     CDS             complement(29226..30284)
                     /gene="vorB"
                     /locus_tag="PLR13_00715"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQC17878.1"
                     /translation="MNKVLMSGNHVIGEAAIRAGCRFYAGYPITPQNELTEYMAEHIR
                     HAEGGIFIQSESEVAAINMIAGASAAGLRVMTSSSSPGISLKQEGISSMAACELPGVI
                     VNIMRGGPGLGNIRPSQGDYFQATRGGGHGDYRTIVLAPASGQELSDLTMEGFDLADK
                     YRNPVMILADGMMGQMMEPVLFKKPKPRKYSEKFMLRGAGTGKSKFIHGLILDPIDME
                     EHNWKLARKYEVITQKEPRFEEHKIEDAKMVIVAYGTAARIAKGAIKRLRAQGVKVGL
                     FRPVTLWPFPELRLRSLSEIVKHYLVFEMSTGQMLEDVRLSLQGYADIKFHGRPGGAV
                     PTPAELANVIAKFYEKID"
     gene            complement(30277..30486)
                     /locus_tag="PLR13_00720"
     CDS             complement(30277..30486)
                     /locus_tag="PLR13_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC17879.1"
                     /translation="MKGYIVINKEICKECLLCINFCPKGHIVTTTEYNALSYHPVGID
                     ETKECNGCGICATMCPEVAIEVYRE"
     gene            complement(30595..>31632)
                     /gene="ribD"
                     /locus_tag="PLR13_00725"
     CDS             complement(30595..>31632)
                     /gene="ribD"
                     /locus_tag="PLR13_00725"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQC17880.1"
                     /translation="ARGCVSPNPMVGAVLVKNGRIIGKGYHQCFGGYHAEINAIQNAA
                     GRVGGSTLYVTLEPCCHEGKTPPCVDAIIHNKIARVVIGTIDSNPLVCREGVNRLIHS
                     GIDVKTGILEEECRLLNETFFHYMETGLPFVTIKYAQTLDGRIATSTGNSQWISSPAS
                     LRLSHRLRAEHDAILVGRQTVERDNPELTVRLVRGRNPLRVIVDSALKVSPDARIFQN
                     LSAAPTLVATLKTASDSRYKKLAALGVTMLTVSADRDGRVDLKKLFKRLAAKGISSVM
                     IEGGAQIITSVLKRNLANRLVTVIAPKIIGCGIEAVGDLNIRHLDLAMKITVKKISVR
                     GHDIIVDGRLT"
     assembly_gap    31633..31732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      31749..31839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            31922..32689
                     /locus_tag="PLR13_00730"
     CDS             31922..32689
                     /locus_tag="PLR13_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HQC17881.1"
                     /translation="MSFVMNTLAFVFGAAIGSFLNVCIFRLPAEKSIVTPASRCPYCL
                     HPIRFYDNIPIISFILLGGKCRDCESRIAWRYPLVELTMALLSVALFMKFGLSLNFFV
                     FFIFTAVLMVITFIDLDHQIIPDILTLPGIPLFFLAAVFIVRIPWLEAFLGFLIGSGV
                     LFVIALGYEFIAKREGMGGGDIKLLAMIGAFLGWKSLIFILLFSSLMGAVIGIAAMVI
                     HKQDTKYAIPFGPFLSAAAIAYIFFGQCYMNLFLMGY"
     assembly_gap    32914..33013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33153..33587
                     /locus_tag="PLR13_00735"
     CDS             33153..33587
                     /locus_tag="PLR13_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17882.1"
                     /translation="MLHTTCNNKGMGMVEVLIAIFLTSVAVLSILALQPSGWKAMAKS
                     DFAGRAAEVLYNTLEDYQVRISNPCNAVTTGNQAQQTVTTSGETDAIQGDITYTVDVN
                     IVQTNVDPQTYVVTVTVTWPGNTRGIAESLSITRQETYKFGC"
     gene            33591..34322
                     /locus_tag="PLR13_00740"
     CDS             33591..34322
                     /locus_tag="PLR13_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQC17883.1"
                     /translation="MMRNKHGFTLVELIVALFVGSLVLVAIYSTVNIAQRSSSGLERR
                     VSAQQDARSALELMAAEIRMASFNPLSRDNIWIDVNNFDSDCAGASFVNQNYRGIRQA
                     AANAIAIEMDINGDGSIDDPNEIIRYRYDSANRYITRSTNCGGAQAFLGDTNANQNIK
                     TVLVENNNAGIPVFRYFNGSGTDISANVVDNPADPLIGIPGVRRIEITLVADTSNADV
                     GSNNRRRIIYSTSVIPRNHFALIYE"
     gene            34327..34875
                     /locus_tag="PLR13_00745"
     CDS             34327..34875
                     /locus_tag="PLR13_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17884.1"
                     /translation="MFAKRKKYLKNEKGFALVAALIACLILLALGLLVINMSTGDLFT
                     TSAVIGNKKSLAAAESGISKIIADVDPDNWTTTEHYTAATDGSGNLCGTDAFNHGLYD
                     FIDITGGTDPNTEFAVCIPRPSAQPPLPASGYGLSGGGSSAVGYAYMRYDSTVVGRNT
                     SHDAEAKVDVGVGFGPVPLGGN"
     gene            34889..>38782
                     /locus_tag="PLR13_00750"
     CDS             34889..>38782
                     /locus_tag="PLR13_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HQC17885.1"
                     /translation="MRGKKFLIMIVAFIIILSAGISQAADEDLWTVVTEPDALIVLDL
                     SGSMAWIPQGDNPKFYSCGSYCGEPYYAHPIVSGTPSTLYVEAASCVSDGPYTTTPPS
                     GGGMPTSNPWVLGTNCSIDGPFSLAPGSDYLNIYSTVWVTNSASCTSTTQPFYKTRPW
                     YWGYRECSVATYFPDTVYTETNCSTGPFYKTSGSGHSTPCRKYRQCLAFTPGYTASDC
                     ATGPFYKTPGEGHMTECTATSACATPEIVYTETDCATGPFYKTSGPGHTTICQNCNKE
                     CYPGSSYKYSDTASCDGPFYNDNDTGTRINCSRIAIAKRAIFELFDDTSDNSIASTDV
                     TSLGMRIGFMRYYNCGSEDNDTTHSDPWNYGCIKLSWSMTQADNTTTTPYANIYCNDA
                     TCAEGVSACSSSEPTRECVAGYGITGGTPIGDAVKEARKFLSYHKGLDPAASCRQKSI
                     ILITDGEDTFSCPNQPNYGCGNGSTTCSSQRRAPIYYAKKAAEAGFKTYVVGFGADMP
                     TALQDTLNWAAYYGQTRNPNATQSGDTNAVTVGTDPCTSGTDPLNKTLRGYAFMAANP
                     QELVNAIRTALAMIHEANYSFSSQASVAAARVQEENYIYEVSFDPKNNSGASKEPFWT
                     GHLKKYQIGDDGSIITPHCWDAGVQLRERPAADRNMWTLKNGYMTSFNTANITAADLA
                     VATDARRNEVVGFYRGEPAYNLENWKLGDLFHTNPVAVKTPSKFFFDPRECSSTSFNS
                     HRSAHERTAMNGNQVVIAGGNDGQLHAFRAGNGTDCTSGGEELWSFIPPNLLQKIAPI
                     AHNSHDDRVSLSAHDFFIDGPLQVSDVWLPASWSSGTTKSASDWKTIVTFTLGQGSGN
                     YLWSRSSNCYSASASDFSATYDATNYPYYCGLHAMNVTSSTSTTPTYLWHLMPTSSHA
                     PYLGQAWSKMQTGRIKVGGEERWVGFVGGGYSAATCSGSCNTPATGSAGKGFYVVDLR
                     NGNIIWSYTRANNANMIYSFPASPLPLDLDNDQFIDTVYLGDLGGNMWRFRLCAKDAN
                     CSECGRADYTSSPCTSCTTSDWTGSLMFTSTDAERGANMATPSNAHKQIFTMAQATYD
                     DNHNLWIFFGTGENNDPTYKPTEANPDTSETKNRLYAVKEDPNFIATYSSSQLENITS
                     SRYTDSSEKHGWYINLSTNPLTRSDTTVISNPIGEKMISDPAIFGGMVYYATYVPDQE
                     EDSACGRSGDAFLYKLNYKTGAGGDANPNDASDIGTRTSYMGHGIGSSILVSYAPEYS
                     DADIYATASGGAGTGALTQTMGKAPKASSMTNVLYW"
     assembly_gap    38783..38882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38958..40622
                     /gene="argS"
                     /locus_tag="PLR13_00755"
     CDS             38958..40622
                     /gene="argS"
                     /locus_tag="PLR13_00755"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQC17886.1"
                     /translation="MKNKIINIMENALNRARQKGELPDVHLPYPEVEPPANREHGDYA
                     ANAAMILASQMKQNPRKIAQTILENIVDNEQIIEKAQIAGPGFINFFLKENVWHRAMK
                     VIEEQNDRYGRLDSGQGKNVQVEFVSANPTGPLHIGHARGAVVGDVIAGLLHAAGYNV
                     SREYYINDAGNQMNNLGRSVLLRYREICGHPIEFPENCYQGDYIKEIARDILRQEGEK
                     YLESEESVAIPYFTAVAGKTILDEIKVDLKDFGVVFDEYFSEKELYKDDGVNQLLHDL
                     RRKGFIYSEGETLWFKSTAFGDEKDRVVIRQNGDPTYFAADIAYHHHKYSRGFDMLID
                     VWGADHHGYMPRLWAGIQALGYDKPSLKIILVQLVNLLRAGEPVAMSTRSGEFVTLRE
                     VLDEVGRDAARYNFLMRRSDSHLDFDLELAKKQSSENPVYYVQYAHARICSIIRTAKE
                     RDIALPVYSDIESAVLKEADEMILIKMLVRYPELISGAANALEPHRITFYLNELAGVF
                     HSYYNKYKVVSDDATLTAARLFLVMMVKIVLQNALNILGVNAPEEM"
     gene            40716..>41052
                     /locus_tag="PLR13_00760"
     CDS             40716..>41052
                     /locus_tag="PLR13_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17887.1"
                     /translation="MATQNTKKFELKIGKTGVLIILGGMTGLLCAAFLLGVDIGKNIE
                     TYPGEIASLPQKALAMVWRPAKIKMAEQNVTQTRAPGTLPAEDNIDLTYHDTLMKKKG
                     LDETETFPEK"
     assembly_gap    41053..41152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41153..41644
                     /locus_tag="PLR13_00765"
     CDS             <41153..41644
                     /locus_tag="PLR13_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQC17888.1"
                     /translation="DTLMKKKGLDETETFPEKTTPEAKAINEEELLKGKFHIETAVPI
                     KPEAITPSKEEPAKKEMAALKESSPSPAGVTESRPDQAKFIVQVASLKDKSTAYRITG
                     KIAALGFQTKIVKIDVKGQGSMFRVFATGFKEKSQAQQAVKKINARTGMHCIIKKVDV
                     RTN"
     gene            41674..43005
                     /gene="ffh"
                     /locus_tag="PLR13_00770"
     CDS             41674..43005
                     /gene="ffh"
                     /locus_tag="PLR13_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQC17889.1"
                     /translation="MFESLSEKLDNIFKKLKGRGVLNEENINASLKEIRMALLEADVN
                     FKVAKDFIEDVRVKAVGREVLESITPGQQIVKIVHDRLVELMGGSSATIKFGARIPSV
                     IMLVGLQGCGKTTTAAKLALLLSQEKKKICLVSADVYRPAAIEQLALLGKQIHTGVFE
                     DKNLKDPVEICAQAVEFAKKNGYEVLLIDTAGRLHIDNLLMEELQKIKSRVNPAEIIY
                     VADAMTGQDAVNVGVKFNELIGIDGVIMTKMDGDARGGSALSLKSVIGKPIKFVGVGE
                     KIDNLEVFHPERMASRILGMGDILSLVEKAQANIDKKTALELEKSIRKNSFSLEDFRN
                     QLVQIKKMGSIKDIMGMIPGLGKMKALKDMEPDDKQLVKITAIIDSMTRKERHNYLII
                     DGRRRKRIAMGSGTTVQDVNLLLKNYMEIRKMMKKFNKKDGIKSLMRNFPF"
     gene            43071..43325
                     /gene="rpsP"
                     /locus_tag="PLR13_00775"
     CDS             43071..43325
                     /gene="rpsP"
                     /locus_tag="PLR13_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQC17890.1"
                     /translation="MAVKIRLARMGAKGKPFYRIVAADSEFPRDGRFLEILGNYDPKK
                     NPAEVLVKEERIRKWLQKGAKATLTVANLLKAKGIEAKIK"
     gene            43411..43641
                     /locus_tag="PLR13_00780"
     CDS             43411..43641
                     /locus_tag="PLR13_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQC17891.1"
                     /translation="MKELIKYISQALVDYPDKVEVTEIIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRMILSAASAKAHKRAVLEIIE"
     gene            43649..44149
                     /gene="rimM"
                     /locus_tag="PLR13_00785"
     CDS             43649..44149
                     /gene="rimM"
                     /locus_tag="PLR13_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQC17892.1"
                     /translation="MDLFEVGEIVKTRGLCGCLKILSYLKSGNKISKPDVVYIEKTPG
                     QKNRFCLNKMEQSRNALFIELEGINDVNSAGHLIGGRVYFSKDVLKKLPAGEYYVHEI
                     IGLDVYSDAGDYLGKIESVIPTGSNDVYVCRKAKEEILLPAISDVIKRIDLDQRIMTV
                     KIPDGL"
     gene            44152..44892
                     /gene="trmD"
                     /locus_tag="PLR13_00790"
     CDS             44152..44892
                     /gene="trmD"
                     /locus_tag="PLR13_00790"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQC17893.1"
                     /translation="MIHFDILSIFPDMFTSPLGCSLLKKAQEKGIIHVECHDIRKWTS
                     GRHNMTDDAPYGGGCGMVMKLEPVEKALSAIRKKHQEEPTVILMTPQGEVLNQQIATE
                     LSEKKRIVIICGRYEGVDERIRQHLVDREISIGDYILTGGELPAMVLIDAVSRLLPEF
                     LGNPESTFTESFSQNLLEYPQYTRPAVYKGWKVPEVLLSGNHSEIELWRKTESLRKTR
                     RKRPDLLKKMKLSDEDRKLLEKIKLEDI"
     gene            44972..45526
                     /locus_tag="PLR13_00795"
     CDS             44972..45526
                     /locus_tag="PLR13_00795"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQC17894.1"
                     /translation="MLYIALLHYPVYDKNGRIVTTAIANMDIHDIARVAKTYGAAGFY
                     IVNPAARQRRLAQDIVNHWQEGFGAQYNPHRQNAFSLIHIRESLQNVVDDIVRRTGRK
                     PKSVATGAGPAQGDVLTFAELKDCLKKENDVYMLLFGTGSGISEKLIHESDFRLEPIK
                     GMAGYNHLSVRSAVAVIMDRITRD"
     gene            45558..45905
                     /gene="rplS"
                     /locus_tag="PLR13_00800"
     CDS             45558..45905
                     /gene="rplS"
                     /locus_tag="PLR13_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQC17895.1"
                     /translation="MNILELTEKEQTRGDIPAFKTGDTVKVYVRIIEGQKQRIQAFEG
                     VVIRKRHGNSRANFTVRKISYGVGVERTFPLHSPIIDQIDVVSRGKVRRSRLYYLREL
                     RGKKARIKDAAKS"
     gene            45978..46595
                     /locus_tag="PLR13_00805"
     CDS             45978..46595
                     /locus_tag="PLR13_00805"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQC17896.1"
                     /translation="MDTFEKNARKEGYHFIAGVDEVGRGPLAGPVVAAAVVFPPDYIH
                     SEIKDSKTLSSQKREKLYNVIENDALAIGMKVVDVETIDHVNILQASLQAMRDSVLEL
                     DVSPDYLLVDGLYPVPIITPQKPVVRGDSLSVSIAAASIIAKVTRDRIMEMYHRQFPQ
                     YNFRKNKGYGTREHLNAIRTFGICKIHRKSFHVKNLHQASFEFNG"
     gene            46605..46976
                     /locus_tag="PLR13_00810"
     CDS             46605..46976
                     /locus_tag="PLR13_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQC17897.1"
                     /translation="MTKIITGKKGERLAADFLTGNGYQILETNFRCPLGEIDIIARDH
                     QEIVFVEVKTRKSHALGYPEQAVGIQKQKKLSQLALWYLQAKKMSDKKARFDVVAVTL
                     AVQANEINLIKNAFDFVDACP"
     gene            complement(46983..48359)
                     /locus_tag="PLR13_00815"
     CDS             complement(46983..48359)
                     /locus_tag="PLR13_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQC17898.1"
                     /translation="MAIRKKILCIGAGYVGGPSMAMIAHKCPDYRVTVVDINPERIDA
                     WNSEQLPVFEPGLDTIVKKTRGKNLFFRKDIENQIRDNDIIFVSVNTPTKSYGAGAHM
                     AADLQYVEKTARQILKNSQSSKIVIEKSTLPVKTALAMEKILSSRKGKIRFDVLSNPE
                     FMAEGTAVKDLENPDRVLIGSRETPGGLKARDHLIKIYTRWVPLNRIITSSIWSSELS
                     KLVANAFLAQRVSSINSISALCEKTDADIDEVARAVGMDKRIGDRFLKAGIGFGGSCF
                     KKDILNLVYLCNHYGLRDVAAYWENVLRINEYQRERFVNHILHSMFGTLSEKKICILG
                     FAFKADTGDTRESPAFYITQKLVAEQADVVISDPRALKNAAADLSGIKGNVKFVDDPY
                     RAAADCHAVLILTDWDLYRRLDYRKIYRSMIKPAFLFDGRNILDHQQCFSIGFNVYPI
                     GKRALTHF"
     gene            complement(48381..49325)
                     /locus_tag="PLR13_00820"
     CDS             complement(48381..49325)
                     /locus_tag="PLR13_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC17899.1"
                     /translation="MTKRILITGGAGFIGSHLCDKLLAQGHDVICLDNFFTGDKNNIA
                     HLLGHKRFELIRHDIINPVYLEVEEIYNLACPASPVHYQYNPIKTIKTNVMGVINVLG
                     IAKRTKAKILQASTSEVYGDPDVHPQNESYWGKVNPVGIRSCYDEGKRAAECLMMDYR
                     RQNGVNTKIVRIFNTYGPRMALHDGRVVSNFVIQALQNKVITVYGDGSHTRSFCFVDD
                     MITGLIRMMNAPDDFYGPVNLGNPEEFTILELAQTVIRLTNSRSRIEFLPLPQDDPVQ
                     RRPDISLAKAGIGWSPQISLEEGLLKTIDYFRQVLKNT"
     assembly_gap    49494..49593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49594..49815
                     /locus_tag="PLR13_00825"
     CDS             <49594..49815
                     /locus_tag="PLR13_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17900.1"
                     /translation="IVVDGRSYENGSIAKDVYTLVDVIEDHQIGDKIYEDILKRADEI
                     VERIVRRIVPEIAERVIKEEIEKIKNEPS"
     gene            49837..50430
                     /locus_tag="PLR13_00830"
     CDS             49837..50430
                     /locus_tag="PLR13_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17901.1"
                     /translation="MTYFKNIVFLVLGMLILSGCFGNTQKSIKDETAKLSIKSIAILP
                     IETATDADVKDAQFLRTRIFEEIFFKGYPKISPEDVDQRLGQAPGNADDKTSSMPPQA
                     LTDKIDAETVMSCSLKKNTQYKLFYASTTADVSCVLRRTETDDVVWKTQTHAAETSFA
                     LGQKRLEIKAHEAVEPLINKILKKIMETLPDGPNFIH"
     gene            50488..51036
                     /locus_tag="PLR13_00835"
     CDS             50488..51036
                     /locus_tag="PLR13_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQC17902.1"
                     /translation="MNIPNLISLLRIILVPVIVIFLIQEEYANALIAFSIAGITDALD
                     GALARLLKKQTELGAFLDPLADKILLSTSFIALSIFGLIPGWLTVIVISRDLIIVIGI
                     ITLTMMSVTYEIKPVFISKITTALQIGTVFIALFVKIFPFELMRIDFINVLSWGTAFF
                     TVVSGLVYIFKGIKFLNHSSEK"
     gene            51119..51193
                     /locus_tag="PLR13_00840"
     tRNA            51119..51193
                     /locus_tag="PLR13_00840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51151..51153,aa:Val,seq:gac)
     gene            51292..53205
                     /gene="thrS"
                     /locus_tag="PLR13_00845"
     CDS             51292..53205
                     /gene="thrS"
                     /locus_tag="PLR13_00845"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQC17903.1"
                     /translation="MTIKITFPDHQELSFNRSTPAAEAVSAWKKEALAHTVAVKINGI
                     PADLSRPLDRDATIDLIDLSSAEGLSILRHSISHVMAQAVQDVFPGAKVSIGPSIEDG
                     FYYDFEYHETFTTDDFPKIEKRMKEITKADNPFVREELSREAAVALFEEREENYKVEL
                     INDLSHDVKTVSLYKQGGYVDLCRGPHIPSTGMIKAFKLLSVAGAYWRGNENNKMLQR
                     IYGTGFATENELQGYLTLLEEARKRDHRRLGRELDLFQVNDEAGAGLIIFHPKGMLLR
                     YLIEEWERKEHLKRGYDMVMGPQILKVDLWKKSGHFDHYRENMYFTEVDEQVYGIKPM
                     NCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKTGVLHGLLRVRQFTQDDAHILCTP
                     EQLNSEIRGIADFVSHAMGIFGFEYEVELSTRPEKSIGTDADWDLATSALENALKDNH
                     IAYDVNAGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYIGPDGEKHRP
                     VMLHRVILGAIERFMGVLIEHYAGAFPVWLSPTQCVLLTVTDKHIPYAQTVHDKLIAS
                     GIRSERYFDNEKLGYKIRQAQLQKIPYMLVIGDKEMDAGAVAPRTRDGKNMGAMPVDQ
                     FITLVHQTCDQNK"
     gene            53269..53763
                     /gene="infC"
                     /locus_tag="PLR13_00850"
     CDS             53269..53763
                     /gene="infC"
                     /locus_tag="PLR13_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQC17904.1"
                     /translation="MNREIKSANVRVISEDGKQLGVISLEEALAKAEEAGLDLVEVSA
                     SADTPVCKIMDYGKYRYKQNKKLHDAKKSQTVIQVKEIKLKPKTEEHDVQVKMKHIKK
                     FLKNHDKVKVSMMFRGREIAFTDIGRKLMGKIRDELANECIIDQEPRLEGRNMVMILS
                     PKKQ"
     gene            53782..53979
                     /gene="rpmI"
                     /locus_tag="PLR13_00855"
     CDS             53782..53979
                     /gene="rpmI"
                     /locus_tag="PLR13_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQC17905.1"
                     /translation="MPKLKTHRGAAKRFSLTAKGKIKRSKAFASHILTKKTTKRKRTL
                     RKSTIVDSANAREIKKLIPYM"
     gene            54028..54378
                     /gene="rplT"
                     /locus_tag="PLR13_00860"
     CDS             54028..54378
                     /gene="rplT"
                     /locus_tag="PLR13_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQC17906.1"
                     /translation="MSRIKRSVNARKRRKKVLKLAKGFFGARSRLLRTATEAVNKAMK
                     YAYRDRRARKREFRQLWIARINAAASDNNITYSRLIDGMKKSGIELDRKILAELAVND
                     PQGFAQVVTIAKGK"
     gene            54388..55404
                     /gene="pheS"
                     /locus_tag="PLR13_00865"
     CDS             54388..55404
                     /gene="pheS"
                     /locus_tag="PLR13_00865"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQC17907.1"
                     /translation="MMKELQQLESDALSELSTATSEEQVLTVKTKYLGRKGLLTGLLR
                     NISRVPDEEKPLFGKRCNELKNSLNARLDQAIEQYTSRNKEQALFKEKIDVTLPGRSV
                     NCGRVHPVVQIRREICSVFSSFGFSIVEGPEIELDYYNFEALNIPKDHPARDMQDTFY
                     IDENIVLRTHTSPVQIRVMEKIKPPLRILSPGKVYRRDSDVSHTPMFHQIEGLLVDKN
                     VTFGDLKGILSAFLKKIFGSSTMLRFRPSFFPFTEPSAEVDICCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFRNVDYDPDEYSGFAFGLGLERIAMLKYGISDIRLFFENDIRF
                     LKQF"
     gene            55419..57830
                     /gene="pheT"
                     /locus_tag="PLR13_00870"
     CDS             55419..57830
                     /gene="pheT"
                     /locus_tag="PLR13_00870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQC17908.1"
                     /translation="MLVGLTWLKDYVDVQLTAQELADKLTMAGLEVDEIKKVGPSFSG
                     VVVAKIISVGPHPSAERLSLCDVHDGKDLYRIVCGAKNIAPGNIVPLAKVGAVIPGGY
                     TIKSSVLRGEKSDGMLCSEAELEIGSDATGIMQLSPDLVLGQPLEEALNLEDTVLDVN
                     VTPNRPDCLSMIGIAREVAALTGRKIKWPAVRIHEKAPDIHSLTSVKIIDSDLCPRYT
                     ARVIKNIAIAPSPRWMRLRLESAGLRSINNIVDITNFVMLETGQPLHAFDFRFLEEGR
                     IVVRRSLENEEFISLDGKIRTLPADTVLICDGIKPVAIGGIMGGLNSEVKEDTQTVLL
                     ESAYFRPTSIRSSSRKLGMSTDAAFRFERGIDPDGVVRALDRAAQLMVDLSGGSICKN
                     YIDEYPRKISSAGDIPLRLDRIRKIIGTKIAARDVLQILRRLDMTVKPEGKGRYLVTP
                     PTCRVDLEREIDLIEEIVRLYGYDRVPVTLPDVSVNELDVIARLDMEERLRQLLTGSG
                     FTEIVNYSFGTPSAADMLSLPATDERRNTVRIKNPLGEELSTMRTNLIYGLLDTAKRN
                     FNNGAFDLKLFEIGRIFLHRREGELPEEKTMIAGLITGKIRDDFWNSEQTVDFYTLKG
                     ALENIFCDFKIQNCRYAATTDESFLHPGKACHIVLYETVIGAVGEIHPDVLEKLDIRN
                     TIYVFEMNIDILSRVCEQNSLSFNEVSKFPAVIRDAAFVIPDPMEAEQMIDIVLSQME
                     ELLENVSIFDVYKGKGIPEGTKSLGLRFSYRANDRTLTDAEASAVHDRIVQKTVNVTG
                     AKLRS"
     gene            57865..58137
                     /locus_tag="PLR13_00875"
     CDS             57865..58137
                     /locus_tag="PLR13_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQC17909.1"
                     /translation="MTKIDIIQNVHEKLGFSKKDSSDIVEAVFDLIKDCLANGEKVKI
                     SGFGNFMVKEKRARRGRNPQTGQEISITARRVLTFKSSQVLRKALN"
     gene            58233..58577
                     /locus_tag="PLR13_00880"
     CDS             58233..58577
                     /locus_tag="PLR13_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQC17910.1"
                     /translation="METIIPDKAYFRIGEVSKILNVEPYVIRYWETEFKTVNPMRTKT
                     AQRLYRKKDVQELLTIKNLLYAQRFTIDGAKKQLQKLRNTDEPEEKNSEKQKLFLIRI
                     KQELQHIREIVS"
     gene            complement(58626..58907)
                     /locus_tag="PLR13_00885"
     CDS             complement(58626..58907)
                     /locus_tag="PLR13_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HQC17911.1"
                     /translation="MICKTVKAGFDCVFMTKNGCSYTNGSCQVVVEQCEGCGKIFEYE
                     SARYCKVYPDPAGKWLIGRCPTATHVKIEIKEAQKINPLKASKRASKKA"
     gene            59141..59590
                     /gene="dtd"
                     /locus_tag="PLR13_00890"
     CDS             59141..59590
                     /gene="dtd"
                     /locus_tag="PLR13_00890"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQC17912.1"
                     /translation="MRAVIQRVDRACVSINRQVFSSINEGILILLGVEKSDTEADADY
                     ILEKAVNLRIFEDDQDKMNLSLLDTQGDMMVVSQFTLLGNCVKGRRPSFDAAEEPVQA
                     CRLYDYFVRQAKTFIPGLATGKFQEMMKIDLVNDGPVTIILDSRKMF"
     gene            59593..60081
                     /locus_tag="PLR13_00895"
     CDS             59593..60081
                     /locus_tag="PLR13_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HQC17913.1"
                     /translation="MDNFEIRIDKDGLWYYKGAHMFRKDILSIFYEHLRIDDSGRYLI
                     KLGPECCYLAVEDTVFVVLAVFKTKNADNRRDQIEILLNDDTCEILDMNSLRSGKENV
                     LYCRVKIGKFTARFSRKAYYQLAEFIESSEDGSRFFIPLNGQKYFIRQVQDSDACPTR
                     PS"
     gene            60225..61685
                     /gene="guaB"
                     /locus_tag="PLR13_00900"
     CDS             60225..61685
                     /gene="guaB"
                     /locus_tag="PLR13_00900"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQC17914.1"
                     /translation="MLDDTIKESLTFDDVLLVPAASNILPKDVDTTTSLTKNISLKIP
                     IVSAAMDTVTESRTAICMAREGGIGIIHRNMSIEQQAIEVDKVKKSESGMIVDPITID
                     PDRKVSDALKLMNQYSISGVPVVKKGKLVGILTNRDLRFEENLDQPVSNVMTKKNLVT
                     VSSNISLEKSKKLLHEHRIEKLLVVDDEYRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREARVEALLAAGVDVIAIDTSHGHSAGVIKAVKATKSDFPKCELIAGNV
                     ATADGAKALMDAGVDAVKVGVGPGSICTTRIIAGVGVAQMSAIRDAYKIASKKGVPVI
                     ADGGIKYSGDIVKAIGAGAHCVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYLDDIEAPLKTVPEGIEGRVPFRGSLSASILQLIGGLKAGMGYVGCRTIP
                     DMIKKARFVKITQAGLRESHVHDVIITKEAPNYWLD"
     gene            61740..65222
                     /gene="dnaE"
                     /locus_tag="PLR13_00905"
     CDS             61740..65222
                     /gene="dnaE"
                     /locus_tag="PLR13_00905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQC17915.1"
                     /translation="MKHSQFVHLHLHTQYSLLDGMIRLDELFKKVKAYAMPAVAITDH
                     GNMFGAIDFYQQAFKNGIKPIIGTELYVAPKDRKDKTPSSIGESTRHLVVLVKNKQGY
                     KNLIKLNSLAHIEGFYYRPRVDKALLRECADGLIASSACLHGEIASWLVRGNMEEARR
                     AAREYLEIFGEENFYLEIMENGIPEQKIANQGLIELSKELSIPLIATNDCHYLERDHA
                     EAHNVLLCVQTGKTIEDKDRMTMATDQFYVRSPEEMCELFESVPEALENTVKIAERCN
                     MTFEFGKFYLPKFEVKHPEETLEAYLERKAVEGLEKRMPSILKYSTGEENSIRKKYSK
                     RLHEELEIIKKMGFAGYFLIVSDFIKHAKHNDIPVGPGRGSAAGSLVAFAIRITDIDP
                     LRYGLFFERFLNPDRISMPDIDIDFCQERRGEVIKYVTEKYGKDKVTQICTFGKMLAK
                     GVIRDVGRALNIPYGDADRIAKLVPSIPPNITLNEAFKMEPRFEEERKKNPQIDKLLK
                     LSLILEGLNRHSSVHAAGVVISDAPLMERVPLFSPKDDIVSQYTMKDIETVGLTKFDF
                     LGLKTLTVIKNTLQFIRDYKDTEVDINNLPLDDPDTYKLLMKGETDGVFQLESEGMKD
                     LLINFKPDRIEDVIALIAAYRPGPMKMIPDFIARKHGKQQITYELPQLEPILKETYGI
                     ILYQEQVMQIANAIGGYTMSQADTLRKVMGKKKVADMEKEKPRFMEGAKKQKIDDNKA
                     RIIWDQMEKFAEYGFNKSHSAAYAMITYQTAYLKAHYPVEFMAALLTSEKDNRDKIIK
                     HMTSCKDMGINILPPDINESEKGFSISGENIRFGLTAVKNVGEAAIESVIDVRETAKF
                     LSFIDFLNRVDLRKVNKRVIESLIKCGAFDSMGHKRSQLMHSYEEAMEVAQRNQKEKS
                     SNQSSFFDQLESGSSSFENGLSAYELPDMPEWDHKKLLSLEREALGFYITGHPLLPYT
                     DRLKLVTDSDSENLLIRRDKETVTVAGVVSAINERKTKRNDTMSYVTLEDLHGSINAI
                     FFGDLYKTSYNILHEEEPVIVKGTLDISGTEENQKISLIAQEVTPLAEALKNPYKQVR
                     FMVDADRMSAEDISSFISSIRKFQGKYESYMHIMNGKSETIVYLGDDLRLDINERLKK
                     EADNILGQGATIFS"
     gene            65238..65960
                     /locus_tag="PLR13_00910"
     CDS             65238..65960
                     /locus_tag="PLR13_00910"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HQC17916.1"
                     /translation="MPPYEERTYRSLIKNDHLISYNVKISESDLFIRSDTDLSDQAFQ
                     SLANHRHSLQIYIQNHSDFRTSLLPVDEDHLAPAIVRDMIDKSKICGVGPMAGVAGAI
                     SEFVGRDLLSSTKNIIIENGGDIFLQSQDTLTVSIFAGESPLSHKVHLEIKPEETPVG
                     ICTSSASVGPSLSFGSADAVCVLSPSATLADSAASAIGNRVKNNSDIKSALDFGMNIP
                     GIRGIVIICKKEMGIIGSVRLL"
     gene            complement(66223..67179)
                     /locus_tag="PLR13_00915"
     CDS             complement(66223..67179)
                     /locus_tag="PLR13_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQC17917.1"
                     /translation="MAEDYYQVLGVEKKASADDIKKAYRKLALKWHPDKNPNNKTAEE
                     KFKKISEAYAVLSDPEKRNQYDQFGSADQFRQRYSQEDIFRNFDLDEILRSFGFGGIK
                     GGRATFRTNRKRGGYEGEYDDPFAGLFGGAMGGNRARYANMPQKGQDAEYNLSITLEE
                     SVLGAEKKISFQLENRIEDINVKIPAGISAGKKLRLPGKGLSGFNGGPSGDLYLNINV
                     LPHPIFARDGNDLHIEKTIKFTQAALGTTIDVPTLDGTTKRIKIAPGTQNNTKIRMKG
                     FGVPGLKGALKGDQFVKINVDVPRRLTDRQIKIIQQLADDGL"
     gene            complement(67233..67814)
                     /locus_tag="PLR13_00920"
     CDS             complement(67233..67814)
                     /locus_tag="PLR13_00920"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HQC17918.1"
                     /translation="MSENEVKNILFAGVGGQGILRASDIICEVIMEAGFDVKKSEVHG
                     MAQRGGCVTSHVRYGKKVYSPLAEPGSIQTLVSFEKMESLRYLSFLRKDAAIVVNTEE
                     VYPPAVNMGESPYPDDAVEFLQNNYKTVISFDASKLAQKAGNIKTANVILLGALSNHM
                     NIDKSIWERVIKKSFPEKLIKVNLEAFQMGIHA"
     gene            complement(67833..69596)
                     /gene="iorA"
                     /locus_tag="PLR13_00925"
     CDS             complement(67833..69596)
                     /gene="iorA"
                     /locus_tag="PLR13_00925"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HQC17919.1"
                     /translation="MKVLMSGNEAIARGAYEAGIRFATAYPGTPSTEILENFCKYDGV
                     YAEWSTNEKVAVEVAIGAALAGEKAMAVMKHVGVNVAADPVFTVSYTGTNGALVIVTA
                     DDPSLHSSQNEQDNRNYARFAKIPMLEPADAAEAKHYIKMAFEISARFDTPVFFRTTT
                     RVSHSKSVVTLEEPAVYEDKTGFVYNAEKYVMVPHCARIRRAEVEKRQQALKEFAETF
                     PENKVEITNAEAGIITAGMPYNYAKEVFPGYSYLKLGMVYPLPEKMIRDFASKVKKLY
                     VIEELDPFLEEQIKAMGIAVTGKEIFPYTSEFDPGVIRNAIEKQSIKKVSDYKVPLAA
                     RPPNLCPGCPHRGLFYALKKHKVYVHGDIGCYTLSYMKPLEGLHSCICMGASIGMAHG
                     MSKALKEKGKGKVVGVIGDSTFLHSGITPLLDMAYNQSDALIIILDNSTTAMTGMQEH
                     PGTGYTLMGIETKQVKLENLVSVLGIEHVRVVDPIDQKATTSAIKEELTKPGPSVIIA
                     RRPCALFKRSHIKPKPALKVDPDKCEGCRVCIGLNCPPISWKKGATKKGSKRKGVSYI
                     DATLCNGCGLCAQVCKFNAIS"
     gene            complement(69613..70290)
                     /locus_tag="PLR13_00930"
     CDS             complement(69613..70290)
                     /locus_tag="PLR13_00930"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC17920.1"
                     /translation="MAEKLTKQDLEGPDAFQNTVELVKDYISENKKRFYSIVTAVILA
                     SMIAFGIYMYWSNYQDSARAMYARTQSSASGSETQDMTGNNIRIFQELIEKYPHSWSA
                     RMAHYRLGNIYYSLGEYDKAIAHHKNFVSSRISDHAGIKFLALSSIGYCYEARKDYKS
                     ALEYFQKAQKGNNIGFESVGFSNVARIYELMNDRKNALENYKKALEKTADSSKAVFIK
                     YKISSLS"
     gene            complement(70283..70714)
                     /locus_tag="PLR13_00935"
     CDS             complement(70283..70714)
                     /locus_tag="PLR13_00935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQC17921.1"
                     /translation="MSVNNVTKIRVLFADTDAMGVVYHTNYIKWFELGRNELMRQLGV
                     PYSELTKLNLNLPLIKVGCEYLKFATYDQLLAVETEFAYIRKATVGFNSKIWDEDQKH
                     LLAEGYTVHACTNHEGKLRRIPQLLLELVKKYNINTEEAYG"
     gene            complement(70704..71927)
                     /locus_tag="PLR13_00940"
     CDS             complement(70704..71927)
                     /locus_tag="PLR13_00940"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HQC17922.1"
                     /translation="MKYVILLGDGMADYPTDKLGGKTPLECAFTPFMDQIAAEGTLGL
                     VDTVPEGIHPGSDVATLSVLGYDPRENYTGRGPLEAASMGIDLGPDDVAYRCNLVTIG
                     DQDGPEAFMDDFTAGHISSEEAEIIIRDIHRHIGSDHLEFFPGVGYRHLLVWRNAPAN
                     PDTTPPHDITGKPVAEYLPRGNAAADVNAIIRRAADLLKNHPVNAKRRQAGKKEANAI
                     WLWGQGKKPRIVPLTQKYSLSGGMISAVDLLKGIGTIAGLKVCPVQGATGYIDTNYEG
                     KAKMALDVLNFMDFVFVHLEAPDEMGHEGNAEGKIRAIELFDEKIVGPILTKIGAFGH
                     YRIIVLSDHPTPLDLRTHVSDPSPFAVLSSEKKENRAPGMSFNEINAKAGNLLISPGH
                     LLMEKFIKDWKSVVG"
     gene            complement(71962..73272)
                     /locus_tag="PLR13_00945"
     CDS             complement(71962..73272)
                     /locus_tag="PLR13_00945"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQC17923.1"
                     /translation="MKEIPIGLIGFGTIGTGVVKLLRQNEELITKKLGARLVIKKIAD
                     IDITTPREVRVHKGLLTRDAREIINDKDIPIIIELMGGYEPARTFVLEAVANGKHVVT
                     ANKALLATHGNEIFPAAQKNAVNIGFEASVGGTIPIIKTLKESLVANRIISVMGIMNG
                     TCNYILSKMTDDDKPFDMVLKEAQKLGFAEADPTFDIEGIDTAHKMAIILSLAYGRKV
                     NLKDIYLEGITKISREDISFAKELGYRIKLLAIAMLRNNAVEARIHPTMIPSKCLLAN
                     VNQNYNAFHIMGDASGPVFLFGQGAGMMPTASAVFSDVMDSARDVLSGKNGRGPLRSI
                     NESAMMPIRLIPMDHVETKYYFRFTAVDRPGVLSRISGILGKYNISIEACIQKARVKR
                     GAVPIVMTTYKAKESDVQKALKRISKLDILQGETILIRIEDDLN"
     gene            complement(73463..73876)
                     /locus_tag="PLR13_00950"
     CDS             complement(73463..73876)
                     /locus_tag="PLR13_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQC17924.1"
                     /translation="MKKLIWVIPSVLFFLFAGCSAGLHSIETVQLAKTVRSWDGETLP
                     QYLQGQPEVTILRIRIPAGSKLEMHHHPVINAGVLLKGELTVVADDNKTLHLRAGDSI
                     VELVNKKHYGKNEGKEAAEIIVFYAGVENKPITVK"
     gene            complement(73922..74854)
                     /locus_tag="PLR13_00955"
     CDS             complement(73922..74854)
                     /locus_tag="PLR13_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQC17925.1"
                     /translation="MTIKVAAIQLATAVAESEENIRRCERLSLQAVNEGAQWIALPEF
                     FNTGVSWNPNIARAIQSADGEAATFLCGFSKKYRVLIGGSFLCRLPDGQVRNRYQCYA
                     QGRLVGQHDKDIPTMWENYFYEGGDPGDIGVLGLYENVRVGAAVCWEFMRTMTARRLQ
                     NKVDVVIGGSCWWSIPTNFPLFLQKLWEPDNKRNALEVLRDNARILGVPVIHAAHCGE
                     IECAMPGLPMSYTGFYEGNAVILDAEGRILAHRKPEEGEGVVSAEILMMPKQDSREIP
                     QGYWLRKRGILPFFAWHHQRWLGRRWYARHVRGN"
     gene            complement(74859..75800)
                     /locus_tag="PLR13_00960"
     CDS             complement(74859..75800)
                     /locus_tag="PLR13_00960"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQC17926.1"
                     /translation="MMGVRIGIIGIGGVGGYFGGKLAREYEKSDEYEIIFIARGEHLK
                     AIQKNGLQLFTREGDCVVRPRIATNNPDEAGMFDVVFFCTKSYSLENSAEAFISCIGR
                     QTVVIPLLNGVNSSQRLRRVLPHAIVLGGSVYIISHSDGPGIIKQEGGACKLTFGTDH
                     DPADDYRYILDILLQAKINAVLTDDISGVLWIKYLLMCPLGSLTSATGKTYGQIWENP
                     ELRQKARAMMMEVVAVAAAHHVVIQEHEVDKAMAMIAGFGYHSRTSMQLDFEKGRQTE
                     IDALTAYLCGAGRDYGVPTPLHDDVYAQLKSIKEPKE"
     gene            75979..78522
                     /locus_tag="PLR13_00965"
     CDS             75979..78522
                     /locus_tag="PLR13_00965"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQC17927.1"
                     /translation="MRIQSFVVHLRFKSAILFASILCIVISGCTPLLNVSYKKSLPPM
                     EGEQVLPGLSDNVTVKRDNLGIPYIEAQSMDDLIFAMGYISACDRFTQMEGFRLVSQG
                     RLSELLGRATLEMDIYLRALNLNQVAQTLYLSASSDLRRLLNIYSDGVNAYLANVPLP
                     TTLKLAGHQPDPWRPIDSMYVFLVLTLGLSQNLQEEINLLNVAQVVAPEKLAWLFPIY
                     PDEPLPFEEMKKLKGINLSEAAQNIEQLGVIANLTNTLLLPAAAASNNWAVSGKLTKS
                     GKPIVANDTHLPLTMPSIWHMMHLKCPEIDGAGIGIAGVPGIVAGYNRHIGIGMTMVM
                     ADNQDIFLEKLQQKPDGLYFLYKNKWLKANSREESFRIKGQKKAVTRTFYETLHGVLL
                     NDILTDKPRHELVPMPLNPSLGIAYSWAGNETDRSMDVFFNIMLAKSVDEILKYTSQE
                     TSVIPLNLVMADRKNIAWQVTGRFPLRKKGRGLCPSPGWTGEYDWNGFLSPSKHPSLK
                     NPAKGYVGTANHRTVPADFPYILSSSWYYPDRGERIEQLLKNAKNYTLDDAKAMQLDT
                     YSRFVNVIKTALLEQKTLDTMSSVWQNSEKSTAAHQAMALLRDFDGNMRLDSAGAAFC
                     GAFLFSLSDNLFADELGGKNTRAYQSLLHTFLLAYSALHDHLTDRGKRSPLWDDVTTP
                     QKEERDLILAKTVLDAIGILEKRCGKNPDKWQWGKLHTYHWRTDATLFADYMDFINKT
                     GVKFLSGYVDRGPYPAPGDHTTLNVSAYHPGKDFGTWMIPAMRIIVDFGSDEPFVGIN
                     STGQLDNPSSPHYDDGITAFRLGQYQNFPFKEENIKTQYTKILTLVPKK"
     gene            complement(78671..78871)
                     /locus_tag="PLR13_00970"
     CDS             complement(78671..78871)
                     /locus_tag="PLR13_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17928.1"
                     /translation="MMKGKELIPNPYKFPPKTRPKRPLLSKDGSVLIYEKSMSAGKFF
                     LYSPDGNHKLAGSGGLCSAKSF"
     gene            complement(78911..81373)
                     /gene="recG"
                     /locus_tag="PLR13_00975"
     CDS             complement(78911..81373)
                     /gene="recG"
                     /locus_tag="PLR13_00975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQC17929.1"
                     /translation="MDALNEILQKIDQPLNFAFRDNYKNLSNIKDLGRTLITLLSKLD
                     SFAASFGREEINHEIGALREIFSDYDRQKLELKKRKIENAVKIVEKLKTIIHFLNISV
                     PERNADQKTIEKISDLKEALEKLNLPVQYLKGVGPKMASRFANKKIATVEDLLFFLPR
                     TYEDRREIRKINRLEAEKVQSAVGNVISCQYRYYGRKKILETVISDSTANLTAKWFKG
                     QMAYLLGVFKKGTRVIFTGDVRPDYVGKQMIHPDYEILDEKDEENLLNFKRIVPVYSE
                     TEGLYQKYYRKVMNAALMNYSRYVASPIPDSICEKRHLLNVHEALWSVHFPGSQEDID
                     QFISSRSEAHRRLIYDEFFFFQLGMAVKKSGRILDQGLSFKTDGTMLDKFYSSLKFDL
                     TAAQKRVIEEILNDMSKDTAMNRLLQGDVGSGKTIVSMAAMITACENSYQAAIMAPTE
                     ILAKQHFENIRLWAEPLGLKTVLLTGSMANASRGETLRRIQNGEAGIIIGTHALIQEG
                     VDFHKLGLVVIDEQHRFGVIQRATLRSKGINADVLVMTATPIPRTLAMTVYGDLDVSV
                     INEMPPGKKPVHTVVLPESKRDKVYQAIRAELAKGHQAFIVYPLVEESETLDLKDATN
                     MSRHLQQDVFPDYRVGLIHGKMKEKEKDEIMREFMENKIHILVSTTVIEVGIDVPRAS
                     LMVIEHAERFGLSQLHQLRGRVGRRDILSSCILLANYKCSADARKRLKVMETTTDGFT
                     IAEEDLAIRGPGDFLGTRQSGLPDFRIASIIRDARILSEAKEDAFALAARDPFLDRVE
                     HQILKEVLIWKWQGKLDLARTG"
     gene            81549..82598
                     /gene="purM"
                     /locus_tag="PLR13_00980"
     CDS             81549..82598
                     /gene="purM"
                     /locus_tag="PLR13_00980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQC17930.1"
                     /translation="MTDNITYKDAGVDIDIANEFVERIKTSVKTTARKEVVSGIGGFG
                     GLFRLNSVDLKNPVLVSSTDGVGTKLRIAHLLDKHDTIGIDLVAMSVNDVVVQGAEPL
                     FFLDYLATGKIELEKSVSIIEGITEGCRQAGCTLLGGETAEMPGFYAPGEYDLAGFCV
                     GVVESDKLITGSTISVNDRIIGLASSGLHSNGFSLARKVLLEKGKLNVNDKLPGLEKT
                     VGLEMLEPTRIYVKALLNLFKKFTLKGLVHVTGGGFYENIPRIIPQACRAVIGKGSWP
                     VPPIFNIIREIGNIDEKEMFRVFNMGIGMMIIVSDKDCPAVLEYLEILGEKAYPLGLI
                     EIREDKKPQVIISES"
     gene            82608..83222
                     /gene="purN"
                     /locus_tag="PLR13_00985"
     CDS             82608..83222
                     /gene="purN"
                     /locus_tag="PLR13_00985"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQC17931.1"
                     /translation="MADALKLGILISGNGSNLQSIIDHIEQGSLRAVIQIVISNNPDA
                     YGIIRAKKHGLPFCVLKNSDFKNKEEFDSELINTLIKHDVELVTLAGFMRIISPRFLQ
                     AFPGRIMNIHPALLPSFPGLHGQRQAVTYGVKVSGCTVHFVDEGVDTGPIIIQRAVPV
                     RDDDTEDTLAARILKEEHKIYPQAIQLFADGKLEIRGRKVFIHP"
     gene            83288..84724
                     /locus_tag="PLR13_00990"
     CDS             83288..84724
                     /locus_tag="PLR13_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17932.1"
                     /translation="MYDLIVIGDDLSSHVAAAFCAINGLKTALFSEKGTAGSSVAGDL
                     TFDGTITPVSGFAENQIAYLSLKNLGIYPEVRLLNPAYQIILPSQRIDFFHDKEELLK
                     ELAREFPHLEGDLRLFYDVAEKNSNAAGQWISEHPGIQPKKMKDFFDYLKLIPRFVES
                     MFNTIRLKRLMSQNPSFRKVIQAQHALLSFKTDPLNSFFSSWQSCTPLRGTFSFAQGK
                     QDLVDSLIRKIKASDGLYLSGCDCLSIHKDKVIELNYTDDNGNTEKIESRHLIVSTKW
                     QNIGQLFDHKKTFSFGDFLRPAKTAYFPFSIFLGVNPAGLPEKLARHSAIICNPAREL
                     YAHNNLIILESSAKEERMKDPAAKILLSATVFLPDHQEIWSKDNLKTISENIMNQLDH
                     FFPFLKENILFFDPDESMKLSLKLRGTIIPRYQIKNSFFTGFSAKGSKTRFANIYLTG
                     ASLLADAGFEAEILSGLNTANLLISQRK"
     gene            84728..86026
                     /locus_tag="PLR13_00995"
     CDS             84728..86026
                     /locus_tag="PLR13_00995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQC17933.1"
                     /translation="MKDVFSISNLGKRMIVSPLQTGKFTRDGKRALFNTHLDNYRSLT
                     DKKGQPLSVELAGPREKIFFNPADTRAAIVTCGGLCPGINDVIRSITTALYFNYHVKK
                     IVGVKYGLQGLNPHCGHPLISLSPDVVKDISSFGGSILSTSRGPQDVQIIVDYLHRLN
                     INILFCIGGDGTIRAAEKITAEITRRNFKIAVIGIPKTIDNDLHLIQKSFGFDTAIEK
                     AADAIKSAHVEARGMVNGIGLIKIMGRHSGHIAAHAALAQSDANFVLIPEVPFDLEGK
                     NGFLGFLKKRMISRGHAVIIVAEGAGQDLLGTTDRSAEKDASGNARLLDIGSHLKNVI
                     ENYFKKISMEINLKYIEPSYLIRSVPANASDDIYCNMLGQYAVHAGMAGKTGILVGLM
                     KDEYVHLPLAAVASGRKIDPRGDIWLRVLETTGQPASMKN"
     gene            86208..86405
                     /gene="rpmB"
                     /locus_tag="PLR13_01000"
     CDS             86208..86405
                     /gene="rpmB"
                     /locus_tag="PLR13_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQC17934.1"
                     /translation="MSRVCEVCGKKPSVGNAVSHANNKKKAVWYPNLQNLRCVDEKTG
                     SIKRIKVCTRCIRSGFVKKAT"
     gene            complement(86398..87186)
                     /locus_tag="PLR13_01005"
     CDS             complement(86398..87186)
                     /locus_tag="PLR13_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17935.1"
                     /translation="MLTHTYFLQKILGSAEIKNVEPDIFVYNIAPDLLAIHPDITSAR
                     THKLRRLQTLPVEYPKAAYVIYHLLIDDLSHYGYICTEDGDAFDIHSRGYSYRKGNPL
                     IRVIQDIHGIAGKEISCRDAVYQSHLIIEMIYDLVIVRHLRTFKTVEVMVDAIKYTVE
                     NRMEEFTETLRWLYGLEVNQIREVMRSALLFITQESMEQMMTIQGRMHLYQDKFGLPC
                     NDLLLNNELNRLFEQAMDLIDDDELFFNETADVIKESQIISFIR"
     gene            complement(87216..87437)
                     /locus_tag="PLR13_01010"
     CDS             complement(87216..87437)
                     /locus_tag="PLR13_01010"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HQC17936.1"
                     /translation="MMSKYFQLDRKNIVAVQFIIEGYEGLATVTTIDAAVAVIQISIM
                     PDFLREMNCLLDELSKKYHMKELVDDRIR"
     gene            87521..89482
                     /locus_tag="PLR13_01015"
     CDS             87521..89482
                     /locus_tag="PLR13_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HQC17937.1"
                     /translation="MRLVRNTSILLLLFIVSCSPIHPFIKSISESDVASFLQNAPRAE
                     EHPNAGADLLYSYSYVEFFEDGRSISRNLDRIKIFNERGRSFASKSISYREGYQKVKI
                     IFANTIKPDGKIIALDRKDIIDSSEYAGYEFYTDIKVKKFTMPAVEDGCIIEIAYEIE
                     NLKPVLSMDYSETFFCQNFFPIEEDIMEIVLPAGTKLQYENFRTDITPQITSLGNKIK
                     YVFINKKQKEIIPESRMPSLLDRDTFPQISFWTLSNWDIISQWYIKLFKEQMKSSAEL
                     ENFTRELIADQKTDEDKINAIFNFVSQNIRYIAVLLGPHTHKPHQAFEVFEKRYGDCK
                     DKTVLLLTMLKIASIQGLPALVPANGKYFDETIPSMNAFNHIIAVVPLKGKSFWLDAT
                     NETSSYDSPPFMLPTRIFLINDDGSYRFVTTPPLNDKKDYYHLAIRYKIDTEGNAAID
                     YVYNFFGKAAEAIRYSFKYAPPEQRKKFFENRGIEIRDLELGSFSDTRTPFTIKLSGM
                     IRNLAQVLDEETMVLSNIIPLDAYRDITAASIRRYPISLTQSMYAMENNTYQFPPGFK
                     IKKLPQNYIFKKPFRYRKEKYSFTGSEFRVYLETKNEEQNIPLGKMNEFKKYASELQK
                     HESAVKNIIFYKRYQNLDTRKDIDIINHK"
     gene            89502..>91192
                     /gene="recJ"
                     /locus_tag="PLR13_01020"
     CDS             89502..>91192
                     /gene="recJ"
                     /locus_tag="PLR13_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQC17938.1"
                     /translation="MTTQRKLPSLKWKIEEAGNKNDGALLSKEFGIHPIIAQILLNRG
                     IRDVDKARHYLQPSLNDLHSPFLMKDMRQGVARLLKAIHNNEKIMIYGDYDADGITSV
                     VILYKFIREITPVLDYYIPDRIQEGYGLKESAINEFKKNGVSLIITVDCGISDVEQIA
                     YARSLGIDTIVLDHHEISGGLPPAAAAINPNRDDCPYPFKGLAGVGIAFNFLIALRGT
                     LNKEGFWKDNHYPNLREYLDIVTLGTIGDIAPLTDENRIFTKIGLELITEGRRPGIKA
                     LKEVSGVDNQVIDSFKASFSLIPRINAAGRIASPSDAVKLLLTDDLSEARPLAEKLDS
                     YNRHRQLMEKKILSDILEMIGKNRSLEKMNSLVFASDKWHPGVIGIVASRLVELFNRP
                     AFVISIINGVGKGSGRSISGFNIYKGIQQCEHLLLSFGGHYHAAGISIEEANIEKFSA
                     VLDELTGDIAEFSEIASQITIDTECGLEDIDPALLAQMEALAPFGSRNPEPLLLAHNV
                     KASSLVVVGNNHLKMSINSNGTSRDSIWFNMGKHLSALDGASLDIVFTPQINNWNG"
BASE COUNT        24148 a        21508 c        21892 g        22944 t
ORIGIN      
        1 tcattgcccg ggggataacg catgcaagtc aatctcactg aacaggaaat tgtcaaattt
       61 gcctatgtca ccaaagaaat cattgaccag tcgtgggggg caaccctttc caatgcgcaa
      121 atcaattgtt atattgagtg cttctatacc ctcaaagatc tcctggaaca gatagatcgg
      181 gaataccttc agaaacattc ggaaatctcc gattattttc ttacgggaaa aagactgatc
      241 aatgaaaaag acaagctgca atgggaaaaa gaatggcagg tatagcggcc ccggcataaa
      301 cgattctatc gctaagcacg attccaggtt ggacagcttc ttccaaaagc atcagcagtg
      361 ttaaataaca gcaatacgca tttgcaacaa gattcgagaa aacatgaaga ttgtattatt
      421 gtccatgcca gactcgattc cgatgattat gcatgagagt gcatttcacc tgcccaattg
      481 cggaattgcc agcatcggcg ccaaccttga cgagggccat gacgtttacg tggttgatct
      541 gatccggaaa cgcaggcatc ttgcggggta tctgaaaaga ttgcttctta agatccatcc
      601 gaaactggtt ggtttgtcgg cgatggcctg gcagtatgag acctgtattg aaatcatccg
      661 ctttatccgg cttctgcttc cggaggtaaa aatcgccatc ggcggctatc acgccacgct
      721 gatgtatgaa gaaattgcgg catcgccgga gtccggtctg atcgatttca tgatccgcgg
      781 ggaaggggaa gaggcattcc ggcgtctggt caatgcgctg gaaggcagcg atcagttgca
      841 ggatattccc tccctgtctt ttaaacgtcg gggaacgttt tgccataatc caagaggagc
      901 cctgctggat ctttccaggt tgaagattcc catacgcgat aaacgccgtt tgacatccgg
      961 ctatcacgcc gttttttcca aaatcgaggt tatcgagacc tcgcgagggt gcaccaggac
     1021 ctgttccttt tgcagcatgc gggagatgta cggacagacg tttcggacgt ttcccatcga
     1081 aagggttacg gcggatatcg atgatattta ttacaagcat aacacccgcg ccatctttat
     1141 tgtggatgac aacctggttt tggatcccga acgggtgatc gatctttgcg atgcgatcat
     1201 tgcaaaaaat tacaagggct tgagcctgca cgttcaggcc gattgcattt ccattgcggg
     1261 gaacgaaacc atggtcagga aaatgtctct tgcgggcgta aaaaccgttt ttctggggat
     1321 tgaaaatgtc gtgaaaaaaa atctgatggc gtccggtaag ggcgatatcg tggatctttc
     1381 cagaaaagcc gttcggatat gccatcagtt cggcatcatg gtgatcgccg gactgatttt
     1441 cggttttccc gatgatgatg aaaacgatat cgttgataat tatcgctttc tgaaatcgct
     1501 tgacgcggat attccctatt gccagattct cacgccctat ccgaaaacac cggtgcgcca
     1561 gcagttgctg gaaagaggca tggtgacgaa cctgcatgat tacaaactgt acaacggcct
     1621 cagagccaat attaaaacgc ggcatcttga tactcaacaa ctgcagtatc tggtctggct
     1681 gcatcggcag aagatgttcg gctggtggga accatcccgt ctcatgagaa nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attcgcggcg gaagcatcgc cgccggctac
     1861 ggcgtgacga aaagctatgt tgatatttta acccaagcgc tgcttccgga aggggtgtgt
     1921 gtgatcaacc gcgcccgata ccgggaaacc tcttttgacg gcatcgagac ctatccgcag
     1981 gatattgacc ggtacgagcc ggattttctt ctgattcact tcggcgtgga tgatgccttt
     2041 cagtgcgttt atcgttcaga atttcaggaa aatattgtgc agatgattcg tctgagccgc
     2101 aaacgattgc agccggttat ttttctggcg acatcgcata cgtttgataa tcccaatgac
     2161 atggatgccg ttcacatctt ttaccggtcg ctgcggattg ttgcttcaga tctgaagtgt
     2221 gaattcatcc ccgtacacag aagctggaac gcttaccttg aggaacacaa tctttgcagc
     2281 caggacctta cgcaggagga ttcgcgctat ccgaatgaac aaggccatca ggttatcgct
     2341 aatgccatga tgaacgtcct gggtaaattc ttttaaaaat tcatccccgg tgccccagga
     2401 attcatcact ttgcccgggc gggaaatctt ttctgcaggg agtcataatg gtagccggaa
     2461 actgccaagg cccgcaccgc ctgctcggcg gtttcaaaga tcgggatgcg gtgcttggcg
     2521 cggatgccga tggacttggc tatggcgttg ggcgtataga tggcggcggg tttctgataa
     2581 tgcctgcaca tcttcatgag ctccagaagg gtatcgatta caaacgtctg ccagacgctc
     2641 aacagcggca ggaggccgtc cacttcatcg gcggaaagaa ggatttcata aatatcgaga
     2701 taggtctgcg gcaaggcagg accgatatcg accggattct tgatattgaa aacctgcccc
     2761 gcctttttta atttttcctg cgtttccggt ttcaggcgca cgacctgaag atgcgcatcc
     2821 accagcagat ccgctccgat gctgccaaac gctccggaat ttgtgaacat ggcaatattt
     2881 ttgcctttca gtaggggcat ctccgtcagc attttaggca tggaatgcag ttcgtcgatg
     2941 gattgcagac ggatcattcc cgactgtttg caggcgctgt tgaaaatgct gtcgttgttg
     3001 gccatgccgg ccgtgtgaga catggccgcc agggcgcctt cctgtgtgcg tcctgctttg
     3061 taaatcagga tcggtttatg cgactttctg gcagcctcca tcagcttgcg accatttgtg
     3121 acgctttcca gatacagccc gatcacctgc gtttcatccg tctgaaaata atcgatcaga
     3181 tccgcttcat ccacatcgca tttgttgccg atactgacaa ccttgttcac gccgacgaga
     3241 tcgtaaaaaa aggactcaat caccagcacg acaacgccgc cgctctggat gataaaggaa
     3301 atgttacccg gtgcttcgaa tatccggccc atttcgacca ggctgggatt gacgccatag
     3361 aaacagcaga atttcttgcg ggtatccagt gttcccagac agttgggccc catgatccga
     3421 atgccgtggg ctttggcgat ttcgtcgatt ttgcgctgca tggccttgcc gtcactgccg
     3481 ccttcggaaa atccggcgct ttcgatgatg acccgcttga ttcctttccg ggcgcaatcg
     3541 ccgatgatcc tggggacgtt tttggccgct acgataatga cggccagatc cggactttcg
     3601 ggaagatcga gtatcgatga ataacccgga tagccgttca ccttttcgcc tttcgaattg
     3661 accgcgtaaa ctgcaccggg atattgaaga taatccttca gcatgttaca aattgtagct
     3721 ccaaggttaa aaggggtatt ggaggcgccg acaacaacaa cagaatgggg atcgaaaaat
     3781 ttttccatat gcaattcacc tgcagcaaga ttaagctttt ttttctattc aaatccatca
     3841 cgctttgtca agaaagaaaa atgttcaaac cgccgatgac tgtccggtaa tcatggttgc
     3901 ggattttgtt tttatgtaat attagtataa ccgagtttat caggagaaat aaatattgtc
     3961 caagcgcatc attgttctgt ccgaagacat cgccaataag attgccgccg gagaagtcgt
     4021 agagcggccg gcttccatcg tcaaggaatt gctggaaaac gccattgacg ccggggcgac
     4081 cgatatacgg gttgaattgg aaaagggcgg tacgcagtcc atcagggtca ccgacaacgg
     4141 ctcgggcatc gaccacgacg atgtcccgct ggtttttgaa agacatgcca ccagcaagat
     4201 tcatcaattt gaagatattt tcaatgttgt gtcgttcggt tttcgcggcg aagcgatgcc
     4261 gagcattgcc tccgtcgcga aagtagaaat tttaaccaga cggaaaaatg atgtgtccgg
     4321 cacccgggcg attctggacg gcggcgtgat ccgggccatg gatccggccg gaatgcccga
     4381 aggcacgcag gtatcggtca gcggcatctt tgcccatgtt cccgcccgcc gtaaattttt
     4441 aaaaacggaa gcaacggaac agggggcctg tctggatgtg attacccgtc tggccctggc
     4501 ccatccggaa atccgtttca aagtcgaggc aaacgggaag gaaatattcg cggctccgga
     4561 agtccatgac acggccaagc gcattgccat ggtcatgggc ggcgagtttt cggcgcattg
     4621 catggcagtt gatgcacagc aggacgccat gaccctgaag ggatttattt cgcgtccgga
     4681 atactccaaa tcgaattcca aaagcattta tctgtttgtg aataaaagat tcatacggga
     4741 taccagcctg acgcatgccg tattgtcggc ataccggcag attattgagt ccagacgtta
     4801 tcccgccgcg acgctgtttc tggaggtgcc gcccgaagac gtggatgtca acgtccatcc
     4861 tgcgaagatg gaggtgcgct ttaaaaatgc gcatggcgtt tatgaactgg tctcgaaaac
     4921 cattgctcat catttgtccc gtgccgaaac atccagagga cattacctgt atcgtctggc
     4981 gcctgcggaa aaaaaagatt atttccatcg gcagcctttt gtgaatgagc cgtctccgtc
     5041 catgccttcc agacccgctg accggtcgca tgcacaacag ggaattcaaa acattttatt
     5101 gcaccgctca caggtgtcgc aaccgccggc tgcttcagaa ggggatgcgg gtgtccctga
     5161 taaaacaatt ttatttggcg acatgagata tgtgggtcag tacgacggca cctatcttct
     5221 ttttgaggga ccggatagcc tcattctgct tgatcagcat gcagcgcatg aacgcattat
     5281 tctggagaaa ctaaagaaag actccggtaa aaaggcggcc ggtcagtcgc tgctgctgcc
     5341 ggaaattgtc aacctgacac cgggacaaat ggccttgctc aacgatgctc ttgattttct
     5401 cgaaacgatc ggtctggaaa tcgaatgctt cggccgggac gcggttgccg taaagacggt
     5461 gccgacaatg ttatccaaag tgaagatcag cgaaatgata tacgatatcg tcgatcagct
     5521 tgccgaccag aacagaacgc cttctctgca ggaacgcagc gacaaccttc tggcctccct
     5581 ggcctgtaaa gcggcgatca aagccaaccg attgctttcc cgggacgaag tggacgcgct
     5641 gtgccgcgaa cttgcggaaa cgccgtttca tgcaacctgt ccgcacggac gtccgatcac
     5701 cattcagttc tccttaagcg aaatcgaaag actgttcaaa aggaagtaaa aaaaccgaag
     5761 atgaagaaac tgccgctggt tatcatcaac tcaccgaccg ccacggggaa aacggatctg
     5821 gccgtgtatc tggctttgga atgcggcggt gagattatca gcgcggattc catgcaggtc
     5881 taccgttatc tcgatatcgg tacggccaag ccgacgatgg aacagcgccg tgggatcagg
     5941 catcatctga ttgatgtcgt gaatcccgac ggggaattca acgccgcttt atttgccgca
     6001 cgcgcccggg cggtcattga ggaactggtt catcagggga agccggtctt cgttgtcgga
     6061 ggaacggggc tttatatccg ggcattgctg aagggaatta tcgacacccc ggatgtcgat
     6121 gcatccatca gaaatcatta tcggagcctc cgggaaacgc acggcaaaat ctatctttat
     6181 gatttgctcc ggcaaaaaga ttccgcggct gccgcgcaaa tcaatccgaa cgattcggtc
     6241 cgcgtgatcc gggccctgga ggtgctggaa cagaccggcg aatcgatcac ggtgaaacaa
     6301 aaaaaacatt ctttcagcga agaccgttac acaacctgca agattggact cagggtggaa
     6361 cgcggcgaat tgaaggaaag gattgaaaag cggacagatg caatgatccg ggccggcctg
     6421 ctggatgagg tcaaaggcgt tctggcaaga ggatacagag aaaatctgaa atcgctgcaa
     6481 tcgctgggct ataaacagat catcggattc ctcaactggc aatatgactg gcgccactgt
     6541 gtgaatttca ttaaacgcga cacgtggcaa tacgcccggc gtcagatgac ctggttttcc
     6601 gccgataagg aaatcaactg gtttgcgccc gatgcactgg acattctgag aagacatgtt
     6661 aaaatatttc aaaatgaaaa tcaggagact gaatgacccg aagagatcac catacgtccg
     6721 ttgagtattt gtgccggctt cagaggcttt ttgtcgtcgt cctgattgcc ctgccgttga
     6781 atattggcgc ggcaactcat taccactgtg cggaccgaag cggccttcag acgttttccg
     6841 attaccctct ggaggatcag gtttgcaccg aaatggtatc gcaggaggag acggagattc
     6901 caaagacgga tagacccatt gcgcccaatg cggaatcgcc cgagaaaaaa acaaccagaa
     6961 tcgccgtcaa agaaaacggt attttggttc ctgtaacgct cgggtatgac ggccgggagg
     7021 cagacatccg tttggttctg gataccggtg cttccgccac gaccatctac gacaatgtag
     7081 ccggccgatt gctggtaaat ttaaataatg cgccaaaggc gcaggggcgg gttgtcggcg
     7141 gtggaaccat tgatgccaat cgtgtgacgt tcagttatct caggatcggc cccaatacct
     7201 ttcgcgactg gaatattttt gttgtggaaa ataaggacgg tgatacgtca tacgacggtc
     7261 tgctcggcat ggatgtctta agcaccatgc ggtataaaat cgatttcagg aatcagatca
     7321 ttatctggga atgagttttt ataataatgt gtctattgtg caatctttca ccgttttatc
     7381 cggggaacga tgattcgctc acagtctccg tctcgtcatt catgccggag ggacaataac
     7441 ggacaatgca atattgcttg acttaaattg gttctaaaga taataatatc aatctgtttg
     7501 gactatgtgg cgatgatgaa ccgaaaaaga gacagaataa tatttttcac cctgttgatt
     7561 tttgcgtccc tgctgacgtt ggccggctgc gaaccgaaag tacgggttgc ggtaagtcaa
     7621 ctggatacgc cggggcacca tacctacgcc ggcctgaagc ttctggaact cgagaagtat
     7681 tccgatgcga ttagagcctt taaaatggcg acccagctgg acagcaacta ttccaaggct
     7741 tataccggga tggcgctcgt cggcatagcc acaggaaaac tctcggcggc agcggatgat
     7801 cttgaaaaag gaattctgaa cgccaaaaca tcggatgaag agctttttgt caacacggcg
     7861 gctattcggt atcacacggc aaaaaaatcc gatgaaaaat ggctggaaaa ggcaaagtat
     7921 tattttgtcg agaccgtcat gatcgattct caatacgcgc ctgtttacta tttcatgggc
     7981 attgcctatc aggaagcgtt ggaattcgac caggcggcaa aaatgttcaa caaagtcatt
     8041 cagctgaaag ccggtcatat cgaggatgcc aataaccggt tggagttcct ggagctggtg
     8101 cacaaagccc accccgccac gcaagccggg aagcaaatcg cgctcaagga aacggtgagc
     8161 cgggcagata tcgccatgat gctggccgag gagctaaaaa taaaagagct ctatcaaaaa
     8221 tatttgccga aaacggcaca ggaggcgcaa accgaagagg aaaaagttgc cgcgtcgatg
     8281 agccaggctg aacacgttgc aaaagaaatg gaaaaaatgg atcagtttca ttcctacatg
     8341 gttttgcagg agaaagaacc ggtgaaactg ctggccaatg acattgccct tcatcctttg
     8401 aaaaattgca ttgaagccgt cctcgaagca aaagttcacg gtctcgaaaa tgatcccaaa
     8461 ggcaatttta aaccggatga aattctctcc cgcggcgaat tcgcaattat tctcgaagat
     8521 attctgataa aagtatcggg agagagagat atcgccgcca gatacgtcag ctccaattcg
     8581 ttatttccgg atgttcccgc agacatgcct tattttacgc cgattatttc agtgacatca
     8641 cggggaatca tggaggcaaa aaatacaaag acgggcgagt tttccccctt aaaacccatg
     8701 tccggtgttg aagcgcttct ggcggttaaa aagcttaagg agaatttaaa gatcgattaa
     8761 taagttatta aaatataaaa tataaataat aaaggggcga gaagcccttt tcgtttgtag
     8821 acccgaatga ggagtaatga tcgcatggca aaaagagaat tgggcaaggc ttttgaaccg
     8881 gccgcagccg aaaaaagatt atatgaatac tggttgaaaa ataaatattt tcacgctcag
     8941 gataaaagcg aggccgaggc gttttccatc gtcattccgc cgccgaacgt gacgggtatg
     9001 ctgcacatgg ggcatgcgtt gaataatacc ttgcaggaca tcattatccg tttcaagaga
     9061 atgcaggggt tcaacacgct gtggatgccc ggtacggatc acgcaggcat tgccacccag
     9121 aatgtggtgg aacagcagtt gctcaaggaa ggaacatcgc gtcacgacct gggacgtgag
     9181 aaatttatcg agcgggtatg ggaatggcgc cggaaatacg gcggggtaat tatcaatcag
     9241 ttgaaacgtc tgggatgttc ctgcgactgg gaacgggagc gctttacgat ggatgaagga
     9301 ttgtcccatg ccgttcgcga ggtattcgtt cgtctcttca atgaaggcat gatttatcag
     9361 ggcgattata tcgtgaactg gtgtccgcgc tgccacaccg ccatttccga tctggaagtg
     9421 gaattcaaat ctgatgcaag cttcctgtgg aacatccgtt atcccttcgc cgaggggcaa
     9481 ggcgaaattg tggtggcaac gacccggccg gaaaccatgc tcggcgatac tgcagtggct
     9541 gttcacccgg acgacaaacg atacaaaaac ctggtcggca aatacgtcat actgcccttg
     9601 atgaataaga aaataccggt tatcgccgac gattatgtga cgatggattt cggatcgggg
     9661 gccgtaaaaa tcacgccggc atcggatccc aatgattttg ccatggctca gcgacatcat
     9721 ctggagatca tttccgtcat tgacggcaag gggatcatca atgaaaacgg cggagcctat
     9781 aaaggtcagg atcgttatga agcccgcaaa aacattgtca acgatcttca aaagggcgga
     9841 tatctggtcg gtaccgagcc gtatgctcat aatgtcggac aatgctaccg ctgtaaaacc
     9901 gacatcgaac cgatggtctc caaacagtgg tttgtaaaaa tcaagcctct ggccaaacag
     9961 gccaccgccg cggtgatcaa ggggaaaacg aaaatcgttc ccaaaacatg ggaagcaacg
    10021 tattttgact ggatgaacaa catccgcgac tggtgcattt cccgccagct ctggtggggg
    10081 caccgcattc cggtctggta ttgcgacaac tgtaaaaaag tgattgtgtc cacggtggat
    10141 cccgacacct gtcccgattg cggcggcgcc tctctgaagc aggacgaaga tgtcctggat
    10201 acctggttca gttccgcgct ctggcctttt tccacgctgg gctggccgga aaaaacggct
    10261 cctctgaaaa cgttttaccc gacctcgttg ttaatcaccg gattcgacat tctcttcttc
    10321 tgggttgcac gcatgatgat gatgggcctt tacgtgatga aggacgttcc cttcaagcat
    10381 gtgtacctgc atgcgctggt cagggatgaa aaaggcgaga agatgagcaa atccaaaggc
    10441 aacatcattg atccgctctt aatgattgat caatacggaa cggacgcgtt tcgtttcacc
    10501 ctggccgcct ataccgctca gggacgcgat gtgcgccttt cccccgaacg catcgagggc
    10561 tataaattct ttgttaataa aatctggaac gcctccaaat tgacatttgc caatctggac
    10621 gattttgacg aaaccgcaac tgcggaggtt gccgattcgc tggcggattt gtggatcaag
    10681 gcccgtctga ataaagcggt ggaggacgtg acgaccgccc tggatgaata ccgctttaac
    10741 gacgccgccg ccgcggttta ccgttttatc tggcatgaac tgtgcgactg gtatctggaa
    10801 ctgagcaaac cggttttcta cggcaaggga agcgcagaac aaaaacaggc cgcccagaaa
    10861 acgctcggtg ttgtttttcc gactatgctg aaattgttgc atcccttcat gccgtttttg
    10921 acggaagagc tctggcaggt ctttcacggg gacgataaaa attcaatcat gatcagccgg
    10981 tttccggcgg tttgttcgga atggaaaaac gacgcggcgg aaaaagaaat ggaaacgatc
    11041 atggatatga tcaccgccgt ccggaatatc cggggtgaaa tgcgcattgc gccgtcgcaa
    11101 aagctcgatg ttttgatgtc caccgtcgat gaaaaaaccc aaaaaatggt ggagaaacat
    11161 aaagactata ttttaaatct ggccaatctg gaatccttaa ccgttgaaat caatgcggtg
    11221 gaaccaaaag gtgtggccac ggcggttgtc ggctcgacaa aactattcgt tccgattgtc
    11281 ggcatcgttg atctttccgg cgaaatagcc cgtctggaaa aggaactggc caagatatca
    11341 aaagatctgc agcagagttc gaaaaagctg gccaatcgcg attttatgtc aaaagccgcg
    11401 ccggacatca ttcagaaaga ggaagacaag gtgaaggatt tccagaaaaa acacaccact
    11461 ctggaaaacg ctcttaagaa attgaaaggc atgtccgcct gatggaacag aggattgtga
    11521 gaatcattca aaatgcattg gccgaggata tcggatccgg cgatattacg acaggcgcga
    11581 cggtcaatgc ccgaaaaaag ggcagggcgg tggccattgc caaggggaat tttattgttg
    11641 ccggaacgga cgtatttgcc gggacattca ggacgctgga taaaaacatc aaggtccaaa
    11701 aaaggattcc ggaaggatat ccggtaaaga aaggcgacat catcgccgaa gtatccggcc
    11761 gtttggacca tattctaatg gcggaacgcg ttgctctgaa tctgtttcag cggatgtgcg
    11821 gcattgccac gctgacccac cggtatgtca gggcggttcg taaaacgaaa gccggaattc
    11881 tcgacacacg caaaaccgtt cccggattgc gcgttttgga taaaatggcg gtcaaaaccg
    11941 gcggaggaca aaatcaccgg ttcggtcttt acgacggtgt gctcatcaaa gataatcaca
    12001 tcgaggcggc aggcggaatc acagctgcgg taaaggcgca aagaaaaaaa ctttcacgtt
    12061 tcatgaaaat cgaagtagaa acccggaatc tggatgaagt ccgggaggcc cttgagtgcg
    12121 gcgccgatat cattatgctg gataatatga gcgtttcggc catgaaaaga gccgtatcgt
    12181 ttgtagccgg gcgcgcgctt ctggaagcat ccggcaatgt cggcctgaag aatgtgatgg
    12241 atattgcagc caccggcgta gatttcatct cggttgggga acttacccat tccgtgcagg
    12301 cggccgacat ttcactcaaa atcaggtctt catcctgatg gaatgctctt gatgtatgga
    12361 ttggaacatt gcccgattgc aggaaaccct cggcacagga ctattcggcg gccatcttca
    12421 tttttatcct gaaatcgtat cgacaaacga cgaagccatc gcgctggcga tggcgggtgc
    12481 gccggaaggc actgccgttc tggcggatgc gcagactgcc ggaagggggc gctggcggcg
    12541 ctcctggatt tcaccgcccg gggtgaatat ttatacaacc atcgttttaa ggccggaatg
    12601 cgaatcttcc ggggcgtctc agattcctat catggcaggc gtagccgcag cagaagttct
    12661 ggacggcgtt tgtcccggcc gagtaaagct caagtggccc aatgatgtca taatcggccc
    12721 caaaaaagtc tgcggcatcc tgtcccaggc aaaaataaca tcaaaaaaaa ttgattttgc
    12781 cgttgtcggt atcggcatta atgtcaacgc gggtgtgcac cattttccaa agaccattcg
    12841 ggagtcggca acgtctctgt tgatcgagtc gggcggagaa atttcccgtc acgatttgtt
    12901 aattagcctc tatgaaaatc ttgaaaaatg gtataaacag ctactgcaaa aaggatttga
    12961 gcccgtccga aaaaagtggc tcgaatcggc ggcgatgatg ggacggccca tccggatcgc
    13021 gtttaagggt gaaagcacag aaggcatagc caaagggctg ggtgatgacg gttgcctgat
    13081 tcttgtcgtt gagggcaatg aagaaattaa agtctctgcg ggagacgcaa cgattatgag
    13141 gtggaaagaa aatgcttttg gtgattgatg tcggaaatac caacaccgta ctggggcttt
    13201 ttgacggaaa aaatctgatc catgactggc gcatacggac ggaaatcgac cataccattg
    13261 atgaatatgg tgtgctgatt tataacctgt atcagtccag ccggaaaaaa gaaaaaccct
    13321 tcaaaacggt ttccgccatt attatctcat gcgtggttcc tcccatgctc aatattctgg
    13381 aaccgctctg tatcaagtat ttcaatgtca agccgctgat cgtcggacca ggcattaaaa
    13441 ccgggatgcc gatcttctac gataatccca aagaagtcgg tgccgacaga atcgtcaatg
    13501 ccgtggccgc gtacaataaa taccggaagg aatccattat cgtcgatttc ggcacggcca
    13561 caacgtttga ttatgtttcc ccgaaagggg aatatatggg agggtgcatt gctccgggga
    13621 ttgtcatttc cagcgaagcg ctttttgaaa aagcgtcaaa actgccgcgt gtggaattca
    13681 gccggcccaa atccgcgatt accaaagata cggtcagcgc tattcaggcg ggaattatgt
    13741 tcggttacgc cggacttgtc gacggaatcg tcaacaggat caggacggaa gtcgagacaa
    13801 aaccgctggt catcgccacg ggaggcctgg ccaatattat cgcacccgag actaaaacca
    13861 ttgaaaaaat agaagagatg ctcaccctcg acggtttgcg ttttatctac gaactgaata
    13921 aaaaataatt atgctcaatg tcggactgac aggtggtatc gcctgcggaa aatcaaccgt
    13981 cgcgaaaatg tttgttgaaa agggagcgca tctcatagac ttcgacaaac tggcccatcg
    14041 gctgcaggaa ccgggacagc ccgcgtggca gaaagtcgtc gattatttcg gcaggcaaat
    14101 tctccgtccg gatcaaaaaa tagaccgcgt caaactgggc cgcattgtct ttgccgacaa
    14161 aaaaaaactc cgggttttaa acaagctcgt tcatccaccg gtttacaggg aatggcagtt
    14221 gcgcctggaa aaaatcggca ggaaagaaaa acatgccatt gttttgtcgg atgtgccgct
    14281 attattcgag gggaaaaaaa ctcatctttt cgatctcact atcctggttt tgtccgatcc
    14341 ggaggatcag atctgccgtt tgatttcccg taatcgcata tccggagacg acgcccgggc
    14401 acgattaaaa agtcagatgc cgatcggcaa aaaaacggcg ctggcggata ttgtgatcga
    14461 caacagggac agcattgcgc gcacaaaaaa aacggtggat cgggtctgga gagaacttgt
    14521 ccgactggaa aaaaccaaat cggcctgata aacacaggcc gtcattattc gagaacaaat
    14581 cagggaggaa acaatgatcg aaaaaaacgt aaaaacagac ttcagttcaa aaatatccga
    14641 accggtcatc ggtgcgggta cctatgtcca tccgctggcc gcggttatcg gcaatgtcat
    14701 tctgggtgaa aatatcatgg ttgccccgac agcctgcata cgcggcgacg aaggacagcc
    14761 gctgcatgtc ggcgatgatt ccaatgtgca ggacggcgtg atcattcatg ctctggaaac
    14821 agaactggac ggcaaaccgg ttgaaaaaaa ccagagggac gttgacggca aaaaatatgc
    14881 cgtttatgtg gggaagcgtg tttccctggc gcatcaagtg cagattcacg ggccggcggt
    14941 tgtcatggac gatacctttg tcggtatgaa aacgctgatt tttaaatctt ttgtggggaa
    15001 gcattgcgtt attgaaccgg gtgtgacgtt aatgggcgtc acggtggacg acggccgtta
    15061 tgtgccggcg ggatctgtgg tcaaaacgca ggccgacgcg gatgtcctgc cggtcattac
    15121 gccggattat ccgatgaaag aaatgaataa aggtgttttg catgtgaaca aggctctggc
    15181 caaggggtat ctggcagcgg ctaaaaaata acgtcgtaaa aggcatccgg tattttcaac
    15241 ggccggatgc cttgcagaaa tgaaatgttg acacacgctt cggaaattaa ccatcatcat
    15301 acttgtttgt cctgcggaaa aacagacccg gtcagcaaac gacggtattg ttcggtgaaa
    15361 tgccgacaaa atctgcggca gaaactcaac ctgaagaacg gattgctgca ggccttgaat
    15421 acgaaatacg caaccttcta tttttcgaag accatgatca ttctggatgt cgtgccctac
    15481 gggtccaggg agatattccg gtatgccatc aggcgcagag cgggaaacac gcctgccatc
    15541 gatttcagtt ttatgaccaa caatctggga aaggcctggt gggctgtgtc ggacacaacg
    15601 cacagaaatt atcttgcgtc gcagcatgtg ttaaaaatgg cgaagcgtta tacgattacg
    15661 gacaaatcgc agcggccgaa aatagttaaa ataccggcgg tgaaggtgtc agcgctgaat
    15721 tgtctggaca taaaaaaaat cgatctcgca tcctgtgacc tcgacagggt catcaaaagc
    15781 gcctatcgcc gtcaggtgaa aatccatcat cccgatctgg gcggcaaagc ggcagatttc
    15841 aggaagattc acagcgccta caaagagttg ctggcgtggg cggatcatcc tgtctttgtc
    15901 catcggcggg ggtttcccga taaatggttt tacagcgcag aaacccaaaa atgggttcag
    15961 ccgacgccgc tatccgactg atccggccgg attatttccg aaaagactgc aaatcttgag
    16021 gggtgttgat gttcgtgaag aggggtgtat ttccggcaaa gggccggatg tcttcttcgg
    16081 tgagtctgag cgtatgcatg tctttgaata atccggcgat tttcaatcgg tcggcaagga
    16141 tcatcttttt cataacggcc agacagcggc gcgaatagat ggcatgcagg gcctggaatc
    16201 cttcggataa ctcggggacg atgatgtcgt atgatcgccg gcgtccgatc atgtaacgga
    16261 tgaaatcttc attcaggcag ggcatgtcgc aggcggcaac aaaacagtca tcgcttgcgg
    16321 cgtaaaataa tcccgtataa attccgccga gggatccctt gcctttgtag atgtccgtga
    16381 caatcatcgc ctcggaaaat tccacgtacg ccagcggatc attggttacg ataatgatct
    16441 ttgggaaaat tctgcgataa acggacaggg tcctgtccat caggcgcttc ccgtctattt
    16501 ccagaaacgc cttgttgacg cccatgcgtg aatttttccc gccggccaaa ataagtcctg
    16561 tgacgttttc ctgcatatga tcaattccta aagggttgtc cgatggtccg tctcgtcctg
    16621 attccggaat cgcatcacgt tcagcatgaa tatctccagt ttgctgatca cattgggcgg
    16681 aaaaagattc ccgtcgattt caatggagat ggccggatat tttctttccc tggcccaggg
    16741 ggtcaggacg ccctcgatca cccggccgat caggcaggca aacggggaga tgttgacgat
    16801 gccggcataa ccgtccatca tggcggtggc tgcgacaccg ctggagatgc tgatttcgga
    16861 atacagttcg ttgttgacga aatgcttttc agtgttttcc ataatttcat ccatgttgtg
    16921 cggagcgtgc ggaaccaggc cggttgtcga aagaatgtcg cggatgtttt tctccacttt
    16981 ctttttatag acgtgctcga ttttccattt gacgatatcg gaaaattccg cagccagaaa
    17041 ctttttgtac cagggaacga gtttgagacg tttttgcagt tcgtgacggc ggacgaaatc
    17101 acaatagtaa acccactcgg caacattgga gaccttgccg ataataccgc gccggctgaa
    17161 atgcctgatc agttcatcta ctgcaaaatc atcgcgcctg acgtaaatct ctccgacaat
    17221 caatactttc gggcagtcat cgattttttt ctttaagggg attttggcga tcgaagagga
    17281 tatctccttc agaacgggaa gggattgcgt gacatctttt tccgcaacgt cgatcagttt
    17341 ttgccagatg atattgtaat cggaaacggc cttgaccgga tccgccgcac aggtgcgcaa
    17401 cgaagtttca atatccttca tataatcgcc gatgagtacg gcccaccagg catatttgga
    17461 aaattccgtt cccagctcgt tataagaatt gtcggcgtcc agcgtgaaaa ccacgacatt
    17521 ttccagacgc agatctttga aaagattttc atagaaaaca aaatactggc cggttcggca
    17581 gggaccggtt gtggttggca cgaacagaat gtaaatttcg tctttgcggt atttttccga
    17641 cgatagatat ttcaacgtac tgcccagaac gagatgagag ggcaggcatt ccttgccgga
    17701 ggcgtgattg cgtgccattt gcagcgtata gatatccggc acgggcatgg tttccgcatg
    17761 aatacccagc ccgcggatcg tggcggccag cacttccacc gacagtcttc ccatgttgga
    17821 taaaagcagc ttgacgcggg gattgttttt gatgggaatc ttttcgccgg tgagggtgtt
    17881 tcggatatga atgtcttcgc cggaagtgtt gataaacctt agaccgttat cataccggct
    17941 ttctctgatg tcgctgattt tggaacggta accttcgatg atgtccagaa aggcctcgat
    18001 acgcgtatcc acgccggcat cggccgtgtg ggaatccagc tccagaacca gaaagggttt
    18061 catccccatt atccacttca gatagtgaag catgaaggaa tcgggagcgc aggaaaaatt
    18121 ggagatgtat gttacaaaga tattgtcttc gtttttcaaa agcgcggcag ctttcatgtc
    18181 ctgctgaccg tagtaccagt acatattggg ataaatcccc tgatcctcaa atggcaggat
    18241 gtcaaaagga acgacggtga agccgcggga ggtgtatttt cgcggaatac ccatgttcgc
    18301 atcgggtgta aacgcgttgt agggccgccc cagaacggca ataacgggtc tttgggattt
    18361 gcgggaattt tcaattgcta ttttacccag ttcggcgaat ttcctaaagc attccatttg
    18421 cttttccagg gcaacggaaa aagccaggcg ggcgtcctcc tgatcaaaac cgagccggac
    18481 agccatggcg ataaacgatt cggaggcttt ctgtgtcccg tacataaaac tgacaacggg
    18541 agcgaggtat ttcccctccg gaatttctgg aaaggctttt ttaatatagt aaggcaaact
    18601 ctgcgtgatc ggacagaaac aggccggtgt attgctttca tagctttcca tatcgcggaa
    18661 atgcggcaga aaaatataat ccacgttgcg gttgaaaata tcctgaacgg caccgtgggc
    18721 aatttccgcc ggaaaacagt acgtgctttc cacccgggca acgccctcgt gtgtgatgtc
    18781 gctggacagg acggtttcca caccgagcgt atggaaaaac caagcgtaaa gcggccagtg
    18841 cgaatagacg gaaaagcatt tcggaatgcc gacgacgaaa tccttttttc tgatcaggtc
    18901 ggcgggtcgg ggagcgcatt gattgaagag aatgtcgttg cgctgttcga tataatcgac
    18961 aacatcgctt tcatcgaaac tttttttctt cctgatgttg gcgtatttgt tacaacggcc
    19021 gccgaacata tatttgcggc cgttgacatt cagtacgcgt atcgggcaga agttatcgca
    19081 ggcgctgcac tgaaattcct tttcatggag aatctcggtc gacagaatct tttcaatatc
    19141 gtaatctctt ctgctgagaa atccctcatg cagtttctgt cgggcaagaa tgccgacgcc
    19201 gaagcaaccc atcagttccg gatcgggagg caccagaata ttcttgtcca gaagcatggc
    19261 gaaagccaga ggaaccgcct tgttcttggc gacgcctccc tgcaggacga tgttttggcc
    19321 gacggttctg ttgcccacaa cgcggttgag ataattggat acgatggagg taacgatacc
    19381 cgccgtaatg tcttcacgcg acgcgccctg ctgaatggct ttccggacat ccgaattgat
    19441 gaaagcggaa cagtgctccc cgaacttcag gggttccgac gcgccaagcg cgatatcgcc
    19501 gatttcccag gcatgatgaa tgttcagatc gccctgcgcg gattcttcaa gaaaggaacc
    19561 ggttccggcg gaacaggctt cattcattgc atagtcgatc ggcaccttgt ttttcagata
    19621 aacatacttg gcatcctgtc cgccgatttc aaagatggtg tcgatgtctt cctgataaaa
    19681 cgtcgttccc accgcatgag caatgatttc attatacacg ccgggcgttt ccagaaaaac
    19741 gcccagcagt tctctggagg atccggtggt ggcggccagg gttattttaa ttgctcggtc
    19801 gccgatatcc tcccggatct gcttttgcat ctccaagaag caatttttca gggctttgac
    19861 cggatctcca tgcgtgcgtc cgtaaaagga ggccgtaatc tcggaggttt ccatatcaat
    19921 aagacaggct ttggtggtgg ttgaccctcc gtcgaccccg agaatatatt cccttcccgc
    19981 aatgactttt gattttttgg atggaagata atgaactttc ccctcggccg ttttcaagct
    20041 tttgaatctc ttgaactgga ccatatgggt tttgaaaaga tcgttcaagg cgggcagggg
    20101 agtgcccatc gagccggcca gcagggcggc gccgtaggct tcaaagtaaa cagcctgatc
    20161 ggggacgata aaatcatttt ccggcatgcg ttggcggatg aagcggatca gatgcgggtt
    20221 cctggtgacg cccccgacca acagaacttt tttgccggct atttttgctt ttttaatgaa
    20281 atcgatgacc ttggttgcca tgacatcgct caacgaaagc acgatgtcgc ccgtgctggc
    20341 ctcccccttg ttcagacggt gggtacagtc gcttttcatg aagacagaac agcgggagga
    20401 cagtttcaga atccggcttt cttcaggaat ggaatgaata tcgttgagat gcatgttcat
    20461 ccggccgagc tgctgtttga aaaactcgcc cgtgcccgaa gcgcatttgt ttccggaaaa
    20521 gcttgtgatg atttgctgat tttcatcaat gctgtaaacg acaagatctt cgccgcccag
    20581 agaaaccagc gcgttaacgt tgccgtttaa atgccggagc gcttcttcaa tgcaaagcgg
    20641 ctcgatgacg ctgttgacat ttaaaagatt tctcccttcc gtgcccgtgg ccagggttaa
    20701 tacgccgggc gggaagcttc gctcggacaa cgttttttcc agagcgtgaa caaagtttcc
    20761 ttcatggggc tcgacttttt gccagacaat ccgatcatcc tcaagcaaga tgagcttgat
    20821 actggacgat cctatgttaa ttcccaaagt ctgcatgagc tctcatcaat catttttcag
    20881 gtgtatttag tttatttctt ttaaatatta cgccacaatc aaatcgtcaa gttttagatg
    20941 caaaaaagag tctaaaaaaa acaatctttt ttaatatcaa taatatggga ttttccgatc
    21001 ccggacgtga aagcgtgtag aaaaagacgg cgggaaatca ttatttaaaa tgatttcccg
    21061 ccaccggtga tcaatgattt ctaatttttt taatggcctt cttccatagc cccggcaata
    21121 tacatcatgg ccagaagcat gaaaacgagc gtctgtacaa cggatgtaaa aaccattaac
    21181 acaaacacgg gaagaggaac gaaccaggga accagaaaga gaacgaccag aaggacgatg
    21241 tcttcaccct tgatatttcc gaaaagacgg aaagtaaggg aaagcggtcg cgaaatgtgg
    21301 ctgatgattt cgatcggcag cattaagggg gtcagccacc ataccggccc catgaattgt
    21361 ttgaaatatt ttaagccgtg ttccttgatg cccacaacat gggtggaaag aaagacaatc
    21421 aaagccatcg ccgcattggt gttgatattg gatgtcggag attcaaatcc gggaacgatt
    21481 ccgatgaggt tcgacacaag aataaagagc cccagagtgg agatcaaagc aaaaaacttt
    21541 cttccattgt gtcccatgat ttcattcagc agaccatcga atccgccgat aatgacttcc
    21601 atgacgtttt gagtttttcc gggataaact tctgtacgtc tggtggccaa aaaggaaaaa
    21661 aaagctaaaa gagccattac aaacaatgtg ttaaagacaa tcgtcatgtc aatacccaca
    21721 tctttcagcc attgcgtgtg aaaaaatatt aagggttcca tctattttta tacaacctcc
    21781 tcaataaagt ttttttttgc cagggcgata atcatcgtta ccagaatatt gattatcaca
    21841 acggaaagtc cgataagcag tccaacgatg tcaacgacat tattggcgat taaaagatac
    21901 agtacaacgg ccatgatggc caggcggatg taatatttta ccatcacagt gcctttggga
    21961 ttcccttgca ggttttgaaa aataccgcgt aacccccggg ccatccaatg aaaattgaca
    22021 atactgacaa aaccgccgag caatatgccg agagaaaatt ttaacggagc taaaaacagg
    22081 gatgggatga aaataacggc cagaatgatc cagttggtga tttcaatcct tttttggagc
    22141 gggtctttga cgatgaggtt cactttttaa actctttata atggtttctt catccgtaaa
    22201 attcttttga actaaaacat ataaattacg aaatccggcc gctaacccta tgaataaaaa
    22261 aacaaccgta aaaacatgac cggaatcgaa ttttttgtcc aaatacgacc ccagcagaag
    22321 acaaccgaaa actgccagaa tcatggcaat gccgatgctg ctggcaaaag ccatttgcat
    22381 cgcatacttt cgcgtttctt tatccattcg ctgattggct ccttagcacc tgcggccatt
    22441 aaagtcaata aaaaaatgac cggcgttcat ttttttaaca gatgacgatt tttcttatac
    22501 caatcaatcg tcttggtgag tcccgtccga atatctgttt tggcaataaa tccgaattct
    22561 ttcaatgctt tggatgtgtc cagcatacgc ttaggctgtc catcgggctt ggaggaatcc
    22621 cacacaatcc ggccggaaaa tccggtcaat tcggcaatca gagttgtcag atcttttatg
    22681 gaaatttcaa accccgatcc gagattgaca gggtcgcttt tgtcgtacag taacgtcgcc
    22741 agataaatag cttctgcggc atcctccaca tagaagaatt cccgggtggc cgagcccgtg
    22801 ccccacacct ccagctgatc ggattgacga tcgatggcgt caaagcattt tttgattaat
    22861 gcgggaatga catgcgacga acgggaatca aaattatctc ccggcccata cagattgacc
    22921 ggaagcaaaa ataccgaatt aaacccgtat tgttgccggt aagcctgcga ttgcaccagc
    22981 atcatttttt tggcaagtcc atagggggcg ttggtttctt ccggatagcc gttccagatg
    23041 tcttcttcgc ggaaaggcgt cggggtaaac ttggggtagg cgcaaacggt tcccagggcg
    23101 acaaattttt caatttttct aatgtgtgct tcgtggagca actgcgctcc catgattaaa
    23161 ttatcataaa aaagttccgc cggtttttcc tgattgaacc cgatgcctcc cactttggca
    23221 gcaagatgaa tgacgacatc cggcttcgtt tcctcataca ttctccggat cgcgttaatg
    23281 tccgtcaggt tatattccgg aaggtcggct atttcgatat tccggcagcc tttgcttttg
    23341 aattttttca gcagatgcgt tcccagaaat cccctggcgc cggtgatggt aatccgtttt
    23401 tgaggtagca ttggcaaagc cttaatggtt gaaattttga ttattggaaa cgaaacccgc
    23461 atccaggagc gttttttctt tttgggcaag ctccatgtct gatgcgatca tcatatcgac
    23521 aaggttctcg aaggacaccc ggggttgcca gttcaatatt ttttttgctt ttgtcgaatc
    23581 gcccagcaga gcgtcgacct ctgtaggacg gaagtaccgt ttatcgattt ccacataacg
    23641 tttgtaatcc agatcaagtt tttgaaaaac catttctgca aattcccgga cggaatgcgt
    23701 ctctccggtg gcaatgacaa agtcgtccgg cgtgttctgc tgcagcatca gccacatcgc
    23761 ttccacataa tccccggcaa atccccagtc ccgtttggct tcgagatttc ccagatacaa
    23821 cttgtccttg agaccgagtt tgatctgggt tgccgcacgg gtgatttttc tggtcacgaa
    23881 tgtttcgccg cgccggggag attcatgatt gaaaagaatt ccgttggcag caaaaatgcc
    23941 gtaggcatcc cgataattct gcgtgatgta ataggaataa accttggcgg ccgcatacgg
    24001 actgcggggc tggaaaacgg ttttttcatg ctgcggcggc ggtgcggccc cgaacatttc
    24061 gcttgatgac gcctggtaga atttcgtcgt aatgcccgtt ttgcggattg cttccagaat
    24121 tctcagagtt cccagcgcgg tgacgtcgcc ggtgtactcg ggcatgtcga agctcacgcg
    24181 cacatgactc tgtgcgccca gattgtaaat ttcgtcgggt ttgacggaat ggagcaaatc
    24241 catcaattgc gcggagacgc tcaaatcccc gtagtgaaga aacaatcggg cttcgggatc
    24301 atgaaaatca tggtagaggt gatcgatgcg ttcggtattg aatgtgctcg accgtcggat
    24361 caatccgtgc acttcgtatc ccttgtgcag taaaaattcc gctaaataag aaccgtcttg
    24421 tccggtaatg ccggtcagga aagctttttt catctggact cctgaatctt tgaaattatt
    24481 ttatgtcgct tcataccgga ttgaggaatg cttgtcaatg aaacgattaa agatttctca
    24541 atgtttttgc gcaggcctga agggttttct gaagatcttt ttcggtgtgg gcaaaagaca
    24601 gaaagcccgc ctcgtattga gaaggcgcga gccagatgcc gtttttcaac atgccgtgaa
    24661 aaaaccgggc atacatgttt gtgtcggatt gcaatgccgt ttgcagatca acgacagggc
    24721 cttcctgaaa aaaaacggtg aacatcgaaa aaacacggtt gatgcagaca ggaattcctt
    24781 ttttgaaaaa catttcatgc atggcccggc aaagctgatc tgtcttttta tccagagagg
    24841 cgaaggattt cttttttgcc ttcaaaagct gaagcgttgt taaaccggcg atcatcgcca
    24901 cagggttgcc ggaaagcgtt cccgcctgat agacatctcc ggtcggcgcc agccgctcca
    24961 tgattttttt acgtcctccg atcgctccga cgggcaatcc gccgccgatg attttaccca
    25021 gacaggtcaa atccggtgtg atgccgacga ggttctgata cccgccaaac gtaaaacgga
    25081 atccggtaat cacttcgtca tagattaaaa cgattccgtt tttcgctgtc aggactctga
    25141 gttgtttcag aaattcttct cgcggcgcaa cgacgcccat gttgccggcg accggctcaa
    25201 caataacggc agcaagctgc gatccatacc gctttacggt ttttgcaagg gcgttagtat
    25261 cattgtaggg aagactcagc gtgagccggg cgatatcatc aggaactccc ggggaaccgg
    25321 gaatgcccag tgtcgtcggt cccgaaccgg ctttaatgag cacggaatcg ctgtgcccgt
    25381 gataacaacc gtcaaatttt actatgagct tccgtccggt atatccccgg gcaagcctta
    25441 tggccgtcat cgccgcttcc gtgccggagg atgtcatgcg tatccgttcg atcgacggaa
    25501 gagctgagga aatcatttca gcaatggcgg tctcaagcag tgtcggggcg ccgaaactgg
    25561 tcccttgttt caaggctgcc cgaacggctg ccgttacgtc cggatgggcg tggccgagaa
    25621 tcatcggtcc ccatgagagc acgtaatcga tataggcttt tcccaaggta tcgtaaatcc
    25681 tgctgccttt tgctttcagc acaaagcggg gatgaccgcc aaccgatttc caggcgcgta
    25741 ccggagaatt gaccccgccg gcaatcaccc ggttcgcctt ggaaaattga acggacgttg
    25801 ttttcttcat cagaaatact ccggagatgt ttttttgtat tctttgaggc ttttgaggat
    25861 taaataatcc ccgatgcctg tagcatttac catgtcctta atcaatgatg aaatgctccg
    25921 cccgtccgaa tgcagcattg taaagagatt gtatctttcc aaaaacgcgg gatgcctttc
    25981 ataacagtgg gagatgaagg agaaagatgc aaaaagcctt ccggttgtct cggtttggga
    26041 gggcggcaca gaccaaagca ccaaagcgtt ctgtccgtat ccggccttct gatggcgcag
    26101 gattgcgccg aatttccgga tgagcccttg ttcacgaaac tggccaagag cggtcagaat
    26161 ttcttttgcc ccgggcgttg gagcgccgga caatttttta aacgggaatt tcgttaccgg
    26221 aatgtctttc tgaagcaggc gtgcgatttt tttttgtttc gacgttaact ttctcatgaa
    26281 tggattctat actgtatcgc aaaaaaaaat caagttgccc gattaatgaa aatttgtctt
    26341 gacaatttct ttttgtgccg ttaaaagaga agcactcaaa gatcccaaaa aaagatgaaa
    26401 cgcgaaagga ggtgtgaaaa aacatagcag ggatttttgc agtcagtatt ttaaatttaa
    26461 aaggagaaag ttaaatgaaa aagagtttaa tgtatgtcat gttggcaatg gttatggtaa
    26521 tcgttagcgc accttttgtt tttgcggctc cggaagcagc ggctcaggct cccgcgggaa
    26581 cggttgatta tacgaaagca atcgtggcca gcatatttgc attggcagca ggtcttgcca
    26641 tcggactggg tgcaatcggc gccggtttgg gtcttggtca ggcaacaagc ggagcaagca
    26701 atgccgtcgg tagaaacccc gaagctcagg gcaaagtcat gttgaccatg atggtcggta
    26761 tggccatgac ggaatccgtc gctatttatg ctctggttat ttctctggta attctcttcg
    26821 cgaatccgct gttgaagatt atcggaatgt aatttaacac cgctatattt aaaaggggaa
    26881 gaagcaaata acaaaggact tcttcccctt tttttatcnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnncc gttattcctc gacagacact tttgcgcgta aaatcttttt
    27061 ttccgcctgt tggtgtttgt cggtcagaat tttttctttg gctcgtttgc tgcgcttcct
    27121 tttctgccgc cggatttttt cgatcgtttg cctggcttcg gccatcaaac ccttctgctg
    27181 tatttctatt ttatccaaca gtaaccgtct ggccaaaaaa cggtttaatg attgagaacg
    27241 ttctttctgg catttgacgg acaggcctgt cggaaggtga cgcagataaa cgcaggagga
    27301 acttttattg actttttgtc cgccgggacc ggaagaacga ataaaggttt cttcaagatc
    27361 cgtttcggaa accccaagac gtttcatccg gtcctggagc attttttctt tttccgcaga
    27421 tacgggcatg accaatgcaa tgatacgtca tcagggaatg gaataaattt cttcatcaat
    27481 gagatgaatc cagttttgtt caagaatctc tctggcttcg tcaattttgt tgacgccgag
    27541 aatgacaatc gtctcttcgc cgaaacgatt gatggttgtg tagaggtagt ggatgttgat
    27601 ttccgccttg gccagcggtt tgagtatggc gttcatgccg ccggcatgca ggggaacctc
    27661 gaccgccaga accggcgtca cttcgaaatt cagattatag cttttcagga cggcagccgc
    27721 gcgatcggga tcgttgacta cgagacgaac cttgctgtca tcggcaatgc tggccgcaga
    27781 gacggctttg gtgctgatat cttccagatc aagaatatgc gttaaggcgg caaaagagcc
    27841 gggtacatta tttaaaataa ccgaaatttg tttaacaggc atctatctgt cctcaatcgt
    27901 tcttgacgta atcatatccg gcattaaagg ctttacggtt cagttcggcg agtgatttct
    27961 ttttgctgcc catgatcgtt tccagacttt tcaggtagac atcaggagga atcaccgccg
    28021 atactttgat aaaagccccc atcatgattg tattggatac ccggatattg cccaaatcct
    28081 gtgccatatc ggcgcagggc atagagcaca ccttgatatc gctgcgatgc ggtttttctt
    28141 tgatgatgga ggaatttaaa aaaatggttc cgccgctgga aattttattt tgaaacgtaa
    28201 aaagcgacgg ggaattcatg gccaccagaa agtccggctc cgacgccacg ggggacgcaa
    28261 tgtcctcatc ggcaatggcc acggtgcagt tggcggttcc gccgcgcatt tcggctccat
    28321 aggccggaag gtaggtgacc tgatagccct ggctcattcc gctgtgggcg aagctcaccc
    28381 ccatcatcaa aacaccctgg ccgccgaatc ctgaaaaaat cgttttaatc agcatcagcg
    28441 cccctccgca gatttttttg ccgcggcaat attgtcttta taaaccccgg ggggaaacgt
    28501 cttgctcatc acctcatcaa tccatttaca ggaattgaag gctgtcatct tccagttggt
    28561 ggggcagggg gaaagaattt cgatcaggga aaatcccaac ccgtccagtt gacattgaaa
    28621 cgctttttta atggcttttt tcgttttgtt gatattgagc ggcgaattca ccatgcatcg
    28681 ttccagatac gtaacgcctt ccagttgcga aaaaatttca ctgactttga cgggatagcc
    28741 gtccagcgtt ctatgccggc cggcagggga tgtcgtcgtt ttttgttcca gcatcgtggt
    28801 cggagccatc tgtccgcccg tcattccgta aacggcatta ttaatgaata tgatggtgat
    28861 attttcgccg cgattggccg cgtgaaagct ttccgcgatg ccgatggctg ccagatcgcc
    28921 atcgccctga taggaaaata cgacccggtc gggcagagag cgtttgatgg ctgaagccat
    28981 ggccggaccc cgtccatgca gcgcttcgat catatcgaca tcgaagtaat tgtacgccag
    29041 aaccgcgcaa ccggccggcg gaacaccgat ggtgatgccc cggatatcca gctcatcaat
    29101 gacttcggca ataatcctgt gtgcaatact gtgaccgcat cccggacagt agtgaaaaac
    29161 attttccttc aaacttctcg gtcgtttaaa aacagtttta accatatccg ccaacctttg
    29221 catccctagt cgattttctc atagaatttg gcgatgacat tggccagttc cgccggtgtc
    29281 ggcaccgcac cgcccggacg gccgtgaaat ttaatatccg cgtagccctg cagggacagg
    29341 cgcacatcct ccagcatctg ccccgtgctc atttcaaaaa caaggtaatg cttcacgatt
    29401 tccgataaac ttctcagacg aagttccgga aaaggccata aggtgaccgg acggaaaagt
    29461 ccgaccttca ccccctgggc gcgcagccgc ttgatggcgc cttttgcgat gcgggctgca
    29521 gtaccgtaag ccacgatcac catttttgca tcttcgatct tatgttcttc aaaacgaggt
    29581 tctttttgag taataacttc atatttgcgc gcgagtttcc agttatgctc ttccatgtcg
    29641 atcggatcca gaatcaaacc gtgaatgaat ttgcttttgc ctgtgccggc gccgcgcagc
    29701 atgaactttt cgctgtactt gcgaggtttg ggttttttga aaagtaccgg ctccatcatt
    29761 tgtcccatca tgccgtcggc cagaatcatc acgggatttc ggtatttgtc ggcaagatcg
    29821 aaaccctcca tggtcaaatc ggaaagttcc tggccgcttg ccggagccaa tacgatggtt
    29881 cggtaatcgc cgtgtccgcc gccgcgcgtg gcctggaaat aatctccctg ggagggtctg
    29941 atgttgccca gccccggacc gccgcgcatg atattgacga tcacacccgg caattcacag
    30001 gcggccatgg acgaaatgcc ttcctgtttc aatgaaatcc cgggactcga ggaagaggtc
    30061 atcactctta atccggccgc gcttgctccg gcaatcatat tgatcgcggc cacttcgctt
    30121 tcagactgga taaaaattcc gccttccgca tggcggatgt gctcggccat atattcggta
    30181 agttcgtttt gcggggtaat cgggtacccg gcatagaaac ggcatcccgc acggattgcg
    30241 gcttcgccga tgacgtgatt tccggacatt aatactttat tcacgatata cctcaatcgc
    30301 gacttcagga cacatggtgg cgcagattcc acagccgttg cattcctttg tctcatcgat
    30361 gccgaccgga tgatagctca gggcattata ttccgtggtg gtcacgatat gacccttggg
    30421 acaaaaattg atacacaaga ggcattcctt gcagatttcc ttgttaatga cgatatatcc
    30481 cttcaaagtc cgttccctga aaaattaaat ttgcgccagc tatcttgcgt ataatttcga
    30541 gaaagtcaag ttttttaaag caatctcgtt cgtaaattaa aaaaaatcga tccgctacgt
    30601 cagacgaccg tcaacgatga tgtcatgtcc gcgcacggat attttcttga cggtgatttt
    30661 cattgccaag tccagatgcc gtatattcag gtcgccgacc gcttcaatgc cgcagccgat
    30721 tattttgggc gcaatgaccg tgaccaggcg attggcgaga tttcgtttta agacggatgt
    30781 gatgatctgg gcgccgcctt cgatcatcac ggatgaaata ccctttgcgg ccagtctttt
    30841 aaagagtttt ttcaaatcga cacggccgtc tctgtcggcg ctgacggtta acattgtcac
    30901 gcccagggcg gccagttttt tgtatcggga atcggacgct gttttcaggg ttgcgaccag
    30961 cgttggcgcc gcagagagat tctgaaagat tctggcatcg ggagatactt ttaatgccga
    31021 atcaacaatc acccgaaggg gattcctgcc gcggacaaga cgaactgtga gttcgggatt
    31081 gtccctttct accgtttgtc tgcccaccag aatggcgtcg tgttcggctc tgaggcggtg
    31141 cgataatctc agcgacgccg gcgaactgat ccactgagaa tttcccgttg aggttgcaat
    31201 gcgtccatcc agcgtttgag cgtatttaat ggtaacaaag ggcagtcccg tttccatgta
    31261 atgaaaaaaa gtttcattga gcagacggca ttcttcttcc agaatgcccg tcttgacgtc
    31321 gatgccgctg tggataagac gattcacgcc ttcgcggcac accaggggat tggaatcgat
    31381 tgttccgata acaacacggg cgattttatt atgaatgatt gcgtcaacgc agggcggtgt
    31441 tttcccttca tgacaacagg gctccagcgt cacgtaaagg gtcgaacctc caacgcggcc
    31501 tgcggcattt tgaatcgcgt tgatttcggc atgatatccg ccaaagcact gatgatagcc
    31561 tttaccgatg atccgtccgt ttttaacgag gacagccccg accatgggat tcgggctgac
    31621 gcaaccccgc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttaaaat
    31741 aatacaaagc ttgaaaaggg caaaccgtac tgaaaagacg ggacgcaaag ttactgacct
    31801 aagtcttcaa taagaggata gggtggcaga accgcctggc aacttcccgc aagtccagcc
    31861 gtttcataaa aaccaccctg agaatgttag gactaaaaaa catgcatgaa caggaagcat
    31921 catgagtttc gtaatgaaca ctctggcttt tgttttcgga gctgcgatcg gcagttttct
    31981 gaacgtctgt atcttcaggc tgccggctga aaagtccatc gtcacacccg cctcaagatg
    32041 tccctattgt ctgcacccga ttcgtttcta tgataatatt cccattatca gcttcatctt
    32101 gctgggcggc aaatgccgtg actgcgaaag cagaattgcc tggcgttatc cgctggtgga
    32161 gctcacgatg gcactgctgt cggttgcttt atttatgaaa ttcggtttat cattaaattt
    32221 ctttgtcttt ttcattttta cagccgtttt gatggtcatt accttcatcg atctcgatca
    32281 tcagattatt ccggacatac tgactttgcc gggaattccg ctgttttttc tggcggccgt
    32341 atttatcgtg agaatcccgt ggctggaagc atttctcgga tttttaatcg gcagcggcgt
    32401 gctttttgtc attgcgctgg gttatgaatt tattgcaaaa cgcgaaggca tgggcggcgg
    32461 cgacatcaag ctattggcca tgatcggagc atttctgggc tggaaatccc tgatattcat
    32521 tcttcttttc agctcgctga tgggggccgt catcggtatc gcagcaatgg tcattcataa
    32581 acaggataca aaatacgcga tccccttcgg tccgttttta tccgccgccg ccattgccta
    32641 tattttcttc ggccaatgtt acatgaattt atttttaatg ggttattagt ttttaaaaaa
    32701 gcgaaaaagg cccaaagatg gtgaaaaaga caacgtattg atattaagaa ttatatctgg
    32761 aaattgcccg ggagatgttg tagtaattga taatacatca ggaaatacga atcgagaggg
    32821 cggaatattg aaatcataaa gaatttcagg aaaatggcat atcaattgct gtaattattt
    32881 tgaagatgca ttttatgatg aaaacgttaa aaannnnnnn nnnnnnnnnn nnnnnnnnnn
    32941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33001 nnnnnnnnnn nnnattaaca taaaaagcac aacccataat ggaaatgata tagaatttca
    33061 gacaaggggc acaatttcca atggacggat cgagttggaa aacagcaggg ggtcgcaagc
    33121 aataataaca agcaatatca caggtaaaac tcatgttaca tacaacatgc aataacaaag
    33181 gcatgggcat ggtcgaagtg ctcatcgcga tcttcctgac ctcggtggct gtcctgtcta
    33241 ttctggcgct tcaaccatcc ggttggaaag caatggccaa atccgatttt gccgggcgcg
    33301 ctgcggaagt tttatataat actctggaag attatcaggt gaggatttcg aatccctgca
    33361 atgccgtcac caccggcaac caggcacagc aaaccgttac aaccagcggt gagaccgatg
    33421 ccattcaggg ggatattact tatacggtcg atgtcaacat cgtacagacg aatgtggatc
    33481 cgcagacgta tgttgtcacc gtgacggtga cgtggccggg taatacaagg ggtattgcgg
    33541 aaagcctgtc gataacaaga caggagacat acaaattcgg atgttagaat atgatgcgga
    33601 ataagcacgg atttacattg gtggaactga ttgtcgcatt gtttgtcggc agcctggtgc
    33661 tggtggcgat ctactcgacc gtaaacatcg cccagcggtc atcttccggc cttgaacgaa
    33721 gagtcagtgc gcagcaggac gcgcgaagcg ctctggaatt gatggcggcg gaaatcagaa
    33781 tggcttcatt caatcctttg tcgagagata acatctggat cgatgtgaat aatttcgatt
    33841 cggattgcgc cggtgcttca tttgtaaatc agaactatcg gggcatcaga caggcagccg
    33901 caaacgccat cgccatcgaa atggacatca acggcgacgg cagcatcgat gatcctaatg
    33961 aaatcatccg gtaccgttat gattcagcaa accgttacat tacccggtca accaactgcg
    34021 gaggggccca ggcgtttctg ggagatacaa acgcaaatca aaacatcaag acggttctgg
    34081 tggaaaacaa caacgccggc atacccgttt tccgttattt caacggctcc ggcacggata
    34141 tctcggccaa tgtggttgac aatcccgccg atccgctgat cggcattcct ggggttcgcc
    34201 gcatagaaat aacattagtg gccgacacct cgaatgccga tgtcggatcc aataaccgcc
    34261 gacgaattat ttattcaacc agcgttattc cccgaaacca ttttgccctg atttatgagt
    34321 aatgctatgt ttgcaaaacg aaaaaaatat ttaaaaaatg aaaaaggatt tgcgctggta
    34381 gccgctttga ttgcctgtct gatcctgctg gcgctcggcc tgctggtcat caacatgtcc
    34441 accggcgatc tttttaccac ctccgccgtc atcgggaata aaaagtctct ggcggcggcg
    34501 gaaagcggca tttccaaaat aattgccgat gtcgatcccg ataactggac aacaaccgag
    34561 cattacaccg cggccaccga cggttcgggc aacctgtgcg gaacggatgc gtttaatcat
    34621 ggcttatacg attttataga tattacaggc ggcacagatc ccaatacgga atttgcggtt
    34681 tgtatcccgc ggcccagcgc ccagccgccg cttccggcat ccggttacgg cctgagtggc
    34741 gggggatcaa gcgcggtggg atatgcatat atgcgttacg attcaaccgt tgtgggaaga
    34801 aacacctccc atgacgccga agcaaaagtc gatgtcggtg tgggatttgg tccggtaccg
    34861 ctgggaggaa attaaaggag gtaagattat gcgaggaaaa aagtttttga ttatgatcgt
    34921 cgcttttata attattttat ccgccggcat ttcacaggca gccgatgaag atctgtggac
    34981 agtggtaaca gagccggatg cactgattgt tctggaccta tccggaagta tggcgtggat
    35041 accgcaggga gacaatccga agttctattc ctgcggaagc tactgcggtg agccttacta
    35101 tgcacatccg attgtgagcg gcacgccatc aaccctttat gtggaggccg ccagttgtgt
    35161 gtccgatggc ccgtatacca ccacgccgcc atcaggtggc ggtatgccga cctcaaatcc
    35221 ctgggtactg gggacaaatt gcagcattga cggtcctttt tctctggcac cgggatccga
    35281 ttaccttaat atctattcaa cggtatgggt gacaaacagc gcaagctgta catcaacaac
    35341 acaacctttt tataaaaccc gtccctggta ctgggggtat agagaatgtt cagtggctac
    35401 atattttcca gacaccgtct acaccgagac aaactgctcc acagggcctt tctacaaaac
    35461 ctccggatca ggacattcaa cgccctgtag aaaatacagg cagtgtttgg cctttacgcc
    35521 gggttacacg gcgagtgatt gcgccaccgg tcccttctat aaaacgccgg gggaaggtca
    35581 catgacagaa tgcacagcga cctccgcatg cgcgaccccg gaaattgttt atacggaaac
    35641 cgactgcgcc accggcccat tctataaaac ctccggcccc ggacatacaa ccatctgtca
    35701 gaactgcaac aaagaatgtt accccggttc ttcttacaaa tacagcgata cggccagttg
    35761 cgacggtccg ttttacaacg ataacgatac gggaacgaga ataaattgtt ccagaattgc
    35821 cattgccaaa cgcgccatat tcgagctttt tgatgatacc agcgacaaca gcatcgcctc
    35881 aaccgatgtc accagtctgg gcatgcggat aggatttatg cgttattaca attgcggcag
    35941 cgaggacaat gatacaaccc attccgatcc atggaactat ggctgtatca aattgtcctg
    36001 gagcatgacg caagccgata atacgacgac aacgccctac gcaaatatct actgcaatga
    36061 tgcaacctgc gcggaaggcg tttccgcctg ctcgtcgagc gaaccaacga gagaatgtgt
    36121 tgccggctac ggcataaccg gcggtacccc cattggtgac gcagtaaaag aagcaagaaa
    36181 atttctttcc tatcataagg gacttgaccc tgccgcttcc tgccgtcaga aatccattat
    36241 cctgatcacg gatggtgaag ataccttctc ctgcccgaat caaccaaact acggatgcgg
    36301 caatggttcc acaacttgtt catctcagcg cagagctccc atctattacg ccaaaaaagc
    36361 ggcagaggcc gggttcaaga cgtatgttgt gggattcggc gccgacatgc ccaccgcttt
    36421 acaggatact ttaaattggg cggcttacta cgggcagacg cgaaatccca atgccacgca
    36481 atccggcgac acaaatgcgg ttacggttgg caccgacccc tgcaccagcg gcaccgatcc
    36541 ccttaacaaa acactgcgcg gttatgcctt catggcggca aatccccagg aactggtcaa
    36601 cgcgattaga accgctttgg ccatgattca cgaggcaaac tactcctttt cctcgcaggc
    36661 ctcggtggct gcagctcgcg ttcaggagga aaattatatc tatgaagtgt ccttcgatcc
    36721 gaaaaataat tccggtgcaa gcaaagaacc tttctggacc ggacatttaa agaaatatca
    36781 gattggtgat gacggtagca ttatcacgcc gcattgctgg gatgccggtg tgcaattaag
    36841 agaaagaccc gctgccgaca gaaacatgtg gacactgaaa aacggctata tgacgtcttt
    36901 caataccgcc aacatcacgg ctgccgatct ggctgttgcc accgacgcca gacgaaatga
    36961 agtggtcggg ttttacaggg gcgaaccggc atacaatctg gaaaactgga aactgggcga
    37021 tctctttcac accaaccctg tcgctgtcaa gacgccttca aaatttttct tcgatccgag
    37081 ggagtgctca tcaacatcct ttaacagcca ccgcagtgct catgaacgaa cggcaatgaa
    37141 cggcaatcag gtggtgatcg ccggcggcaa cgacggccag ttgcacgctt tccgcgcggg
    37201 aaacggtacg gattgtactt ccggaggaga agaattatgg agtttcattc cgcccaatct
    37261 gttgcagaaa attgccccca tcgcccataa ttcccatgac gaccgcgtat cgctctcggc
    37321 ccacgatttc ttcattgacg gtcccctcca ggtttcggat gtctggcttc cggcttcctg
    37381 gagcagcgga acaactaaga gcgcatcgga ttggaaaacc atcgtgacct ttacgttggg
    37441 gcagggctcc ggaaattatc tctggagcag atcatccaac tgttactccg ccagcgcgtc
    37501 cgatttcagc gccacgtatg acgctaccaa ctatccttac tactgcggcc ttcatgcaat
    37561 gaatgtgacc agttcgacct ccacaacgcc gacctattta tggcatttga tgccgacatc
    37621 gtcgcatgcg ccctatctgg gtcaggcctg gagcaagatg cagaccggac gtattaaagt
    37681 cggcggcgag gaacgatggg ttggatttgt cggcggcggt tacagcgccg caacatgttc
    37741 cggcagttgt aataccccgg ctacaggcag cgcaggaaag ggtttctatg tcgttgattt
    37801 gcgaaacggc aatatcatct ggagctatac acgcgccaat aacgccaata tgatctattc
    37861 tttcccggcc tctccgttgc ctctggatct ggataatgac cagtttatcg ataccgtata
    37921 tctcggagat ctgggcggaa acatgtggcg gttccggctc tgtgccaaag atgcaaattg
    37981 ttccgaatgc ggaagggcgg attacacctc ttcgccctgc acctcctgca caacctccga
    38041 ctggacggga agccttatgt ttacgtcgac tgatgccgaa agaggggcaa acatggcaac
    38101 gccaagcaat gcccataaac agatctttac gatggcgcag gcaacctacg acgacaacca
    38161 caatctgtgg atattcttcg gaaccggcga aaacaatgat ccaacctata aaccgacgga
    38221 agccaatccg gacaccagcg aaacaaaaaa caggctctac gcggttaagg aggaccccaa
    38281 ttttatcgct acgtattcat cctcgcagtt ggaaaacatt acgtcttccc gttatacaga
    38341 ttccagcgaa aagcacggct ggtacattaa tctttcaacc aatcctctga cgcgttcgga
    38401 taccacggtc atttcaaatc ccatcggaga aaagatgatc tcggatccgg caattttcgg
    38461 cggaatggtg tattacgcaa cctatgtgcc cgatcaggag gaagacagcg cctgcggtag
    38521 atccggcgat gcgtttctct ataagctcaa ttacaaaacc ggtgcgggag gagacgcgaa
    38581 tcctaacgat gccagtgata tcggcaccag aaccagttat atggggcatg gcatcggttc
    38641 ctccattctt gtttcttatg ctccggaata ctcggatgcg gatatctatg ccacggccag
    38701 cggcggtgcg ggtaccggtg cgctgaccca gacaatgggg aaggcgccaa aggcttccag
    38761 tatgaccaat gtcctttatt ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38881 nnatggttgg gcttgcgatg acaggcggca cgtgataatg aaagctgctt gtcatttttt
    38941 tacaggttgc aaaaattatg aaaaataaaa tcatcaacat tatggaaaac gctctgaatc
    39001 gtgctcggca gaaaggagag cttcccgatg tccatctgcc ttatccggaa gtcgaaccgc
    39061 ccgccaaccg cgagcacggc gattatgccg ccaatgccgc catgattctg gcgtcgcaga
    39121 tgaagcagaa tccgcggaaa atcgcgcaga cgattctgga aaacatcgtc gataacgaac
    39181 agatcatcga aaaggcccag attgccggcc ccggttttat taatttcttt cttaaggaaa
    39241 acgtctggca ccgggccatg aaggtcattg aagaacaaaa cgatcgctac ggacgcctgg
    39301 attcgggcca ggggaaaaac gttcaggtgg aatttgtcag tgccaatccc acgggaccgc
    39361 tgcatatcgg gcatgctcgc ggcgcggtgg tcggcgatgt catcgccggt ttgttacatg
    39421 cggccggtta taacgtttcc cgcgaatatt acattaacga tgccggaaat cagatgaata
    39481 atctgggcag atcggtgctg ctgcgttacc gggagatttg cggacacccc attgaatttc
    39541 ctgaaaactg ttatcagggc gactatatca aagagattgc ccgggatatt cttcgtcagg
    39601 aaggggaaaa atatcttgag tccgaagagt cagtcgccat tccctatttt accgccgtgg
    39661 caggaaaaac cattcttgac gaaatcaagg tcgatttaaa ggatttcggc gttgtttttg
    39721 atgaatattt cagtgaaaag gaactgtata aagacgacgg cgtaaatcaa ttgctccatg
    39781 atttgcgccg gaaaggcttt atttattccg aaggtgaaac gctctggttt aaatcaacag
    39841 ccttcggcga cgaaaaggac cgggtcgtga tccgccaaaa cggagatccg acgtattttg
    39901 ccgcagatat tgcctatcat catcacaaat attcacgtgg ttttgatatg ctgattgatg
    39961 tctggggcgc cgatcaccac ggctacatgc cgaggctctg ggcgggcatt caggccttgg
    40021 gatacgataa gccatccctg aaaatcattc tggtgcaact ggtaaatctt ttgcgggccg
    40081 gagaaccggt ggccatgtcc acccgctccg gagaatttgt aacgttgcgc gaagtcctcg
    40141 atgaagtcgg aagagatgcc gcccgttata attttctaat gcgacgttcc gacagccatc
    40201 tggattttga tctggaactg gccaaaaaac agtccagcga aaatccggtt tattatgtcc
    40261 agtatgccca tgcccggatt tgcagcatta ttcggacggc aaaagaacgc gatatcgctc
    40321 tgcccgttta cagcgacatc gaatccgccg tgctcaaaga agccgatgaa atgattttga
    40381 tcaagatgct tgtccgttat ccggaattga tttccggagc ggcaaacgct ctggaaccgc
    40441 accgcattac attttacctc aacgaactgg ccggtgtttt tcacagttat tataataaat
    40501 ataaagtcgt ctcagacgat gccacgctga cggcggcaag actttttctg gtgatgatgg
    40561 ttaaaattgt tttacagaac gctttgaata ttctgggggt aaacgctccg gaagaaatgt
    40621 aactttttta aaatccggga aaatcaaccg gttgtttgac tgtaataaca cgctgaaatt
    40681 ttggaaaatg tcatttttcc ggtttgaaaa gattcatggc tacgcaaaat acgaaaaaat
    40741 ttgaattaaa aatagggaag acgggcgtct tgattattct tggagggatg accgggcttt
    40801 tatgcgcggc gtttcttctg ggcgttgata tcgggaaaaa tatcgaaacg tacccgggag
    40861 aaatcgcgtc tctgccgcaa aaagcgctgg ctatggtctg gcgtccggcc aagattaaaa
    40921 tggccgagca aaacgtcacg caaaccagag cccccggaac tcttcccgcc gaagataaca
    40981 tcgatctgac ttatcatgat acgctcatga aaaagaaggg gttggacgag acggaaacat
    41041 ttcccgaaaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatacgct
    41161 catgaaaaag aaggggttgg acgagacgga aacatttccc gaaaaaacga cgccggaagc
    41221 caaagcgatc aatgaagaag aacttttgaa gggcaaattt catattgaaa cggcggtgcc
    41281 gataaaacct gaagcaatca caccctctaa agaagagccc gccaaaaagg aaatggccgc
    41341 tttaaaggaa tcctctccat cgccggcagg cgtaacggaa tcccgtcccg atcaggccaa
    41401 atttatcgtg caggtggctt cgctgaagga caaatcaacg gcgtatcgga taaccgggaa
    41461 aatcgccgcc ctgggttttc aaaccaaaat tgtcaaaatt gacgtgaaag ggcagggaag
    41521 catgttccgg gtttttgcaa cgggatttaa agaaaaaagt caggcgcaac aggccgtcaa
    41581 aaaaatcaat gccaggacgg gcatgcattg cataatcaaa aaagtagatg tcaggacaaa
    41641 ctaaaataat aaggatgaaa tggttgaagg gttatgttcg aaagtctttc tgaaaaactg
    41701 gataatattt ttaaaaaact taaagggcgc ggcgttttaa acgaggaaaa tatcaatgcc
    41761 tcgctcaagg aaatccgcat ggccctcctg gaagcggatg tcaattttaa agtggccaaa
    41821 gatttcatcg aagatgtgcg tgtcaaggcc gtgggccggg aagtcctcga aagcatcacc
    41881 cccggccagc agatcgtcaa gatcgttcat gaccggctgg tggaactcat gggcggcagc
    41941 agcgccacaa taaaattcgg agcgcgcatt ccgtcggtca ttatgctggt cggcctgcag
    42001 ggctgcggta aaacaaccac tgccgccaag ctggccttgc tgttatccca ggaaaagaaa
    42061 aaaatatgtc tggtttctgc ggacgtttat cgtccggcgg caatcgaaca gctggcgctg
    42121 cttggtaaac agatccatac cggcgtattt gaagataaaa acctgaaaga ccccgtggag
    42181 atttgcgctc aggccgtgga attcgcgaaa aaaaacgggt atgaagttct gctgatcgac
    42241 acggcgggcc ggctgcatat cgacaatctt ctcatggaag aactccaaaa aattaaaagc
    42301 agggtcaatc ccgctgaaat tatttatgtc gccgacgcta tgaccggaca ggatgccgtc
    42361 aatgtgggag tcaaattcaa cgaattgatc ggaatcgacg gcgtgattat gaccaaaatg
    42421 gacggcgatg cccgtggcgg cagcgctttg tccttgaaat cggtgatcgg aaagccgatt
    42481 aaatttgtcg gggtgggaga aaaaattgac aatctggaag tttttcatcc cgaaagaatg
    42541 gcctcgcgta ttctgggaat gggcgacatt ctttccctgg tggaaaaggc gcaggccaat
    42601 atcgataaaa aaacggctct ggagctcgaa aaaagcatcc gcaagaacag tttttcgttg
    42661 gaggactttc gcaaccagct ggtgcagatt aaaaaaatgg gatccatcaa ggatattatg
    42721 ggaatgattc ccggcctggg aaaaatgaaa gccctcaaag atatggagcc ggatgataaa
    42781 caactggtca aaattaccgc gatcatcgat tccatgaccc gaaaggagag gcacaattat
    42841 ctgattatcg acggcaggcg gcgcaaaaga atcgctatgg gcagcggaac caccgttcag
    42901 gatgtgaatc ttttactgaa aaattacatg gaaataagaa aaatgatgaa aaaatttaat
    42961 aaaaaagacg gaataaaatc tctgatgcgg aattttccct tttaatataa attaaagtat
    43021 gataaaaaaa tagaaaaatt attattttaa ggaggtaata ggacaaatca atggctgtta
    43081 aaattagact cgcccggatg ggtgccaaag gcaaaccttt ttatcgaatt gtggcagcgg
    43141 acagcgaatt tccccgcgat ggaagatttc tggaaatttt aggcaattac gatcccaaga
    43201 aaaatcctgc ggaagtcctt gtcaaggaag aacgcattcg taaatggttg caaaagggtg
    43261 ccaaggcaac tctgacagtc gctaacttgc tgaaggcaaa ggggatagaa gccaagatca
    43321 aataattttt taatcacaat gtttggggtg tgttctgtat cggaaattat ttctagaagg
    43381 acttaattaa actcagcgga ggttatgacg atgaaggaat tgatcaaata tatatctcag
    43441 gctttggtag attatccgga taaggtggaa gtgactgaaa tcatcggcga acaaacatct
    43501 gttattgaac tgcgcgtggc caaagaagat ctcggtaaag tcatcggcaa acagggaagg
    43561 accgccaagg cgatccggat gattttaagc gcggcttccg ccaaggcgca taagcgagct
    43621 gttttagaaa taatcgaata ggcagaccat ggatctcttt gaagttggag agattgtcaa
    43681 aacccgcggt ctttgcgggt gtttgaaaat tttatcttat cttaaaagcg gaaataaaat
    43741 atcaaaaccc gatgttgtgt atattgaaaa aactccgggg caaaaaaatc gtttttgcct
    43801 gaacaaaatg gaacaatccc gcaacgcgtt atttatagaa ctcgagggga ttaacgacgt
    43861 aaactcggcc gggcatctga tcggcggccg ggtttatttt tctaaagatg ttttaaaaaa
    43921 acttccggcg ggggagtatt acgttcacga aatcatcggc cttgatgttt acagcgacgc
    43981 gggagattac ctcggcaaaa tcgaatcggt tattcccacc gggagcaatg atgtgtatgt
    44041 ctgccggaag gcaaaagagg agatcctcct gccggcaatt tccgacgtta taaaacggat
    44101 agatcttgat cagcggatta tgaccgtcaa aattccggat ggattgtaaa cgtgatacat
    44161 tttgacattc tttccatttt cccggacatg tttacgtcgc ccctcggctg cagtcttctg
    44221 aaaaaagcgc aggaaaaagg gattattcat gtagaatgtc atgacatacg caaatggacc
    44281 agcggcagac ataatatgac cgatgacgct ccctacggcg gcgggtgcgg tatggtgatg
    44341 aagctggagc cggtggaaaa ggcgttgtcg gccatcagaa agaaacacca agaagaaccg
    44401 actgttattc tgatgacgcc tcagggggag gttcttaatc agcagatcgc aacggaactt
    44461 tctgaaaaaa aaaggatcgt catcatctgc gggcgctatg agggcgttga tgaacgcatc
    44521 aggcagcacc tggtggaccg tgaaatttct atcggcgatt atattctgac cggaggggag
    44581 ttgccggcaa tggtgctgat tgatgcggtt tcccgtcttc tgccggaatt ccttggcaat
    44641 ccggaatcga cattcaccga atctttttca cagaatcttc tggagtaccc ccaatacaca
    44701 cggccggccg tctataaggg ctggaaggtg ccggaggtat tgctgtccgg aaatcattct
    44761 gaaatcgaac tgtggcgcaa aacagaatcg ttgaggaaaa cccgtcggaa gcgacccgat
    44821 ttgctcaaaa aaatgaagct ctccgatgag gacagaaaac tgcttgaaaa aattaagctg
    44881 gaagatatat aaaaccttga ttttcagaaa tatttaatgt atgggtttcc gcttgaatgt
    44941 gcgggatcaa tgatgtcaca cggcaaagga aatgctttac atcgctcttt tacattatcc
    45001 ggtatacgat aaaaacggcc ggattgtcac gacggccatt gccaatatgg acattcacga
    45061 cattgcccgg gttgccaaaa cgtatggcgc cgccggtttt tatatcgtca acccggcagc
    45121 aaggcaacgc cgtctggcgc aggacattgt caatcactgg caggaaggat tcggtgcgca
    45181 atataatcct catcgtcaaa acgctttcag cctgatccat atcagagaga gtttgcagaa
    45241 cgttgttgac gacatcgtcc gtcggacagg gcgtaaaccg aagagcgtag cgacgggtgc
    45301 cggtcctgct caaggcgatg ttttaacgtt tgccgaattg aaagattgcc tgaaaaagga
    45361 aaatgacgtt tatatgcttc tttttggcac aggatccgga atctccgaaa aactgattca
    45421 cgagtcggat tttaggttag agccgatcaa agggatggcc ggttataatc atctgtcggt
    45481 acggtccgcc gtagccgtta tcatggacag aatcacgaga gattgatttt tttaagtaaa
    45541 attttcggga gggaaatatg aatattttgg aattgactga gaaagaacaa accagagggg
    45601 atatccccgc ttttaaaaca ggggataccg tcaaagttta tgtcagaatt attgaaggcc
    45661 agaaacagag gattcaggcg tttgaaggtg tcgtcatccg taaaagacac ggaaacagcc
    45721 gggccaattt tacggtcaga aaaatttcct acggtgtcgg tgttgaaaga acgtttccct
    45781 tgcattcgcc gatcatcgat caaattgatg ttgtcagccg gggtaaggtg cgccgctcgc
    45841 gcctctatta tttaagggaa ttacggggca aaaaagccag aattaaagac gcggccaaat
    45901 cgtaaaaatt gttctgcccg agagcggcgt gccctcacgg gtacgcgagc tcttggatcg
    45961 gggatgtatt ttccgaaatg gatacttttg aaaaaaatgc ccgcaaagaa ggatatcatt
    46021 ttattgccgg tgttgatgaa gtgggcagag ggccgttggc cggtccggtc gttgcggctg
    46081 cggtcgtgtt tcctcccgat tatatccatt ctgagataaa agattctaaa acactttcct
    46141 cccaaaaaag agaaaaatta tacaacgtca ttgaaaacga tgccctggcc atcgggatga
    46201 aggtcgttga cgttgaaacg atcgatcatg tgaatattct gcaggcgtct ttacaggcca
    46261 tgcgcgactc cgttctcgag ctcgatgttt cgcccgatta tctgctcgtc gacggtttgt
    46321 atcccgtgcc gatcatcacg ccccagaagc ccgtggttag aggagacagc ctcagcgtct
    46381 ccattgctgc tgcctcaatt atcgccaaag tgacacggga ccggattatg gaaatgtatc
    46441 atcgtcagtt tccgcaatat aatttccgga aaaataaagg ctacggaacc cgggagcatc
    46501 tcaacgccat cagaacattc ggcatctgta aaattcaccg gaaatcattt catgttaaaa
    46561 atcttcatca ggcatcattt gagtttaacg gttaaatgcg aaacatgaca aaaatcatta
    46621 ccgggaaaaa aggggagcgg ctggcggctg attttctgac cggcaacggt tatcagatcc
    46681 tggaaaccaa tttccgctgt cctctggggg agattgacat tatcgcccgg gatcatcagg
    46741 agatcgtttt tgttgaggtc aaaacaagaa aatcgcatgc tctgggatat cccgaacagg
    46801 ctgtcggcat ccaaaagcaa aaaaaattgt ctcaattggc cttgtggtat cttcaagcca
    46861 aaaaaatgtc cgacaagaaa gcacgttttg atgtcgtggc ggtcaccctg gccgttcagg
    46921 ccaatgaaat taatctgata aaaaatgcct tcgattttgt tgacgcctgc ccgtaaacta
    46981 ttttaaaaat gcgtcagcgc ccgttttccg atgggataaa cattgaaccc gatggagaag
    47041 cactgctgat gatccagaat gttgcggccg tcaaaaagaa aggccggctt gatcatggag
    47101 cgatatattt tccggtaatc cagtctgcgg tataaatccc agtccgttaa aatgagcacc
    47161 gcgtgacagt ccgcggcagc tctgtaagga tcgtcgacaa attttacatt tcctttgatt
    47221 cccgacagat ctgcggcggc atttttcaat gcccggggat cggaaataac aacgtcggcc
    47281 tgttccgcca ccagtttttg cgtaatataa aaagcggggc tttccctcgt atcgcctgta
    47341 tcggccttga aagcaaaacc caggatgcag atctttttct ccgacagcgt tccgaacatg
    47401 ctgtgaagaa tatgatttac aaaacgctcc cgctgatatt cgttgatgcg gaggacattt
    47461 tcccagtagg cggcgacgtc acgcaatccg taatgattgc acaaatacac cagattcaga
    47521 atgtcttttt taaaacagga tccgccgaaa ccgatgccgg ccttgagaaa tctatcgccg
    47581 attcgtttat ccatgccgac cgccctggcg acttcatcga tgtcggcgtc tgttttttcg
    47641 cataacgcag atatggaatt gatggaggac acccgctgcg cgaggaaggc gtttgcgacc
    47701 agtttggaca attcgctgct ccagatgctg gaggtgatga tcctgtttaa gggaacccaa
    47761 cgcgtgtaaa ttttgatgag gtgatctctc gcctttaatc cgccaggggt ctcgcgcgag
    47821 ccgatcaaaa cgcggtcggg attttccaga tctttcacgg cggtgccttc ggccatgaat
    47881 tcgggattgg agagaacatc aaaccggatc ttcccttttc gggaggataa aatcttttcc
    47941 atcgccagcg cggtttttac cggcaaggtg cttttttcga ttacgatttt tgacgattgc
    48001 gagttcttta aaatctggcg cgcagttttt tccacatatt gcagatcggc cgccatgtgt
    48061 gctccggcgc cgtagctttt cgtcggggtg ttgacgctga cgaaaataat gtcattgtcc
    48121 cggatttgat tttcaatgtc ttttctgaaa aataaatttt tgccgcgggt tttttttaca
    48181 atcgtatcga gaccgggttc gaaaacgggc aactgctccg aattccacgc atcaatccgt
    48241 tcaggattaa tatccacgac ggtcacccgg taatcggggc atttgtgggc gatcatcgcc
    48301 atggaagggc cgccgacata accggctccg atgcaaagga ttttttttct gatagccatt
    48361 tatttcttac ctctgtgcgt ttatgtattt tttaatacct gacgaaaata gtcaatggtt
    48421 ttgagcaacc cctcctcgag actgatttga ggcgaccagc cgatgccggc ttttgccagc
    48481 gagatatccg gcctgcgttg aacaggatcg tcttgaggca ggggcaggaa ttctattctg
    48541 gagcggctgt ttgtaagccg gataacggtt tgcgcgagtt ccagaatggt aaattcttcc
    48601 gggttgccga gattgaccgg accgtaaaaa tcgtcggggg cattcatcat ccggatgagc
    48661 ccggtaatca tatcgtcgac aaaacagaat gatcgcgtgt gagaaccgtc gccgtagaca
    48721 gtgatgactt tgttctgcag ggcctgaatg acaaaattgc ttaccacgcg gccgtcatgc
    48781 aacgccatcc gcggaccgta ggtgttgaag atgcggacga ttttcgtgtt gacgccgttt
    48841 tgccggcgat aatccatcat caggcattcc gccgctcttt tgccttcatc atagcaactg
    48901 cggatgccga ccggattcac tttcccccag taggattcgt tctgcggatg gacgtccgga
    48961 tcgccgtaca cctccgatgt ggacgcctga agaattttgg cttttgtcct cttggcgatg
    49021 ccgagaacat taattactcc catgacgttg gttttgatcg ttttaatggg attgtactgg
    49081 taatgaaccg gagacgcggg acaggcaaga ttgtatattt cttccacttc cagataaaca
    49141 ggattgatga tgtcatgacg gatcagctca aaacgtttat gtcccagaag atgagcaata
    49201 ttatttttat cgcctgtgaa aaaattatcc agacagatga cgtcatgccc ctgtgccaga
    49261 agtttgtcgc aaaggtgaga accgataaaa cctgcaccac ctgtaatcag tatccgtttc
    49321 gtcatttttt caattcacaa tttatttggc gaatattaat atgaaataat tcgggaatgt
    49381 caacaattaa aatagcatat tgatggtatt ttttcatgcc gtccgattat gatatatatc
    49441 ttgaactgtt ttatgctcta tgataacaaa ccaacacgct gcaaatttac cgtnnnnnnn
    49501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattgttg ttgacggacg gagttacgaa
    49621 aacggctcca tagcaaaaga cgtgtatact ctggtggatg tcattgaaga tcatcagatc
    49681 ggggataaaa tctatgaaga cattctcaaa agagccgacg agatcgtgga acgtattgtc
    49741 cgcaggatcg ttccggaaat agcggaaaga gtgatcaagg aagaaattga aaaaatcaaa
    49801 aatgaaccgt catgacgaat agagtttaaa gaggatatga cctatttcaa aaatatcgtt
    49861 tttcttgttc tggggatgct gattctgagc ggatgcttcg gcaatacgca aaaatcaatc
    49921 aaggatgaga cggcaaaact cagcataaaa tccattgcca tccttccgat agaaaccgcc
    49981 acggatgcgg acgttaaaga cgctcagttc ctccgtacca ggattttcga agaaattttt
    50041 tttaagggct accccaagat atccccggaa gacgtggatc aacgcctggg ccaggcgccc
    50101 ggcaatgccg acgacaagac atcttccatg ccgccgcagg ctctgacgga taagatagac
    50161 gcggaaacgg tcatgtcctg ctccttgaaa aaaaatacgc aatataaatt gttttatgcg
    50221 tcgacaaccg ctgacgtgag ttgtgtgttg cgccgcacag aaacggatga cgttgtttgg
    50281 aaaacgcaga cacatgccgc agaaacgagt tttgcgttgg gccaaaagag actggaaatc
    50341 aaggcgcatg aagcggttga accgctgatc aataaaattc tgaaaaaaat aatggaaaca
    50401 ttgcccgacg ggccgaactt tattcattaa gcaaagcaaa acagcattac gaaaagaccg
    50461 gcaaattatt tttgaaatga ctatgcgatg aatattccca atctgatatc tttgctcaga
    50521 attatactgg tcccggtgat tgtcatattc ctcattcagg aagaatatgc aaacgcgttg
    50581 attgcatttt ccatcgccgg tatcacggat gcactggacg gtgcgctggc cagactcttg
    50641 aagaagcaaa ccgaactggg cgcttttctg gatccgcttg ctgataaaat tcttttatcg
    50701 acgagtttta ttgccttgtc cattttcgga ttgattccgg gatggctgac tgtcattgtc
    50761 atcagcaggg atctgatcat tgtcatcggt atcatcacat taacgatgat gtcggtaacg
    50821 tatgaaatta aaccggtttt tatcagcaaa atcacaacag ctttacaaat agggaccgtt
    50881 ttcattgctc tttttgttaa aatatttcca tttgaattaa tgcgcattga tttcattaac
    50941 gttctttcgt ggggcaccgc tttttttacg gttgtctccg gattggtata tatattcaag
    51001 ggaataaagt ttttaaatca cagctcagaa aaataggcta ataaataaga catttagctt
    51061 gacacggcaa tattttgctg ctatcaacac gtcgttaaaa aaatccggtt tttaccgtag
    51121 gcaagtagct cagggggaga gcgccatcct gacgcggtgg atgtccaggg ttcaaatccc
    51181 tgcttgccta ccatttttat ataaattacg tcttgatttc ataataacaa agggcatcag
    51241 caagacagtg atgccctttt aaaatgaagt taaggtgctt tgacgaatga tatgactata
    51301 aaaattactt ttccagatca tcaggaatta tccttcaacc ggtcaacgcc ggcggctgaa
    51361 gccgtcagtg cctggaaaaa ggaagctctg gcccataccg tggccgtaaa aatcaacggc
    51421 atcccggctg atctcagccg tccgcttgac cgagacgcca caattgatct catcgacctg
    51481 tcctccgctg aagggttgtc tatcttgagg cacagtatat ctcacgtcat ggctcaggct
    51541 gttcaggatg tatttccggg tgcgaaagtg agtatcggcc cgtccatcga agacggcttt
    51601 tactacgatt ttgaatatca tgaaaccttc acgacggatg attttccgaa aatcgaaaaa
    51661 cggatgaaag aaatcacaaa ggcggataat ccttttgtcc gggaagaact gtcccgtgaa
    51721 gcagccgttg cgttattcga ggaaagagaa gaaaattaca aagttgaatt aatcaacgat
    51781 ctgtctcatg acgtcaagac ggtcagcctt tataaacagg gcggctatgt tgatttatgc
    51841 cgcggccccc acatcccgtc aacgggaatg atcaaagcct tcaagctgct cagcgttgcc
    51901 ggagcctact ggcgaggcaa tgaaaacaac aaaatgctgc agcgtatcta cggaactggt
    51961 tttgctactg aaaatgagct tcagggatat ttgaccctgc tggaagaagc ccgtaaaaga
    52021 gatcatcggc gtctgggcag ggaactggat ctgtttcagg tcaacgatga agccggagca
    52081 ggcctgatta ttttccatcc caaggggatg ctgctgcgtt atctgattga ggaatgggaa
    52141 agaaaggaac atctcaaacg cggatacgat atggtcatgg gaccgcagat cctcaaggtg
    52201 gatctctgga aaaaatccgg gcattttgac cattaccggg aaaatatgta ttttactgaa
    52261 gtggatgagc aggtttacgg tatcaagccg atgaactgtc tgtcccatat gctgatctat
    52321 aaatcaaaaa tcagaagtta tcgggatttg ccgctgcgtt attttgaact gggcacggtg
    52381 catcgtcatg aaaagacggg ggttctgcac ggtttgctga gagtgcgaca attcacccag
    52441 gatgatgctc atattttatg cacacccgaa cagctgaatt cggaaatccg gggcattgcc
    52501 gattttgtca gccatgccat gggaattttc ggatttgaat atgaggtgga attaagcacc
    52561 cggccggaaa aatcaatcgg caccgacgcc gattgggatc tggccacctc tgcgctggaa
    52621 aacgcgctca aggacaatca cattgcctat gatgtgaatg ccggcgacgg tgctttctac
    52681 ggcccgaaga tcgatttcaa gctcaaagac gccctgaaaa gaaaatggca gtgcgccacg
    52741 atccaatgcg acttcacgct tccggaaaga ttcgatttaa gctatatcgg accggacgga
    52801 gaaaagcatc gtccggtcat gctgcaccgg gtgattctcg gcgcgattga gcgtttcatg
    52861 ggggtgctca tcgaacatta cgccggcgcg tttcccgtct ggctgtcgcc gacccagtgc
    52921 gttttgctga cggtcaccga caagcatatc ccttacgctc agacagtgca tgataagctg
    52981 atcgcatccg gcatccgctc cgagcgatat tttgacaatg agaaactggg gtataagata
    53041 cgacaggctc agctgcagaa aattccctat atgcttgtta tcggcgacaa ggaaatggac
    53101 gcgggcgccg ttgcaccccg cacacgcgac ggaaagaaca tgggcgccat gcccgttgat
    53161 cagttcatta cattagtaca tcaaacttgt gatcaaaata aataataaac gacattcgga
    53221 taagataaat ttaatttcag gaggtgacct atagtaaagg atttaagaat caacagagaa
    53281 atcaaatcgg ccaatgttcg cgtcatcagt gaagacggta aacagttggg cgttatttcc
    53341 ctggaagagg cacttgccaa ggcggaagaa gccggtctgg atttggtgga ggtttccgca
    53401 tctgccgata ccccggtctg taaaataatg gattacggga agtatcgcta caagcagaac
    53461 aagaaactgc atgatgccaa aaagagccag accgtaattc aggttaagga aattaaatta
    53521 aaaccgaaaa cggaagaaca tgatgtgcag gtcaagatga aacacatcaa aaagtttttg
    53581 aagaatcatg ataaagtaaa ggtctccatg atgttcagag gcagagaaat cgcatttacc
    53641 gatatcggca gaaagctgat gggaaaaata cgggatgaac tcgccaatga gtgtatcatc
    53701 gatcaggagc cccgtctgga aggacgcaat atggtgatga ttctttcacc aaaaaaacaa
    53761 taaatatgga aaggtgatca catgccaaaa ttaaaaacac accgaggagc tgcaaaacgc
    53821 ttttcactga ccgccaaagg gaagattaaa cgcagcaaag cgtttgccag tcacatcctc
    53881 acgaaaaaaa cgacaaagag aaaaaggacg ttaagaaaat caacgattgt tgattcggca
    53941 aacgcaagag aaattaaaaa acttattccc tacatgtaat ggaccgggga taatttacga
    54001 gttatctgta aatgaggaga aaaagaaatg tccaggataa aaaggagcgt caacgctcgt
    54061 aaaagaagaa aaaaggtttt gaaactggcc aaagggttct ttggcgcccg cagccgcttg
    54121 cttcgaacgg ctacggaagc ggttaacaag gcgatgaaat acgcgtaccg tgaccgccgg
    54181 gcgagaaagc gggaattccg ccagttatgg attgcgcgca tcaatgccgc cgcaagcgat
    54241 aacaacatta cgtacagccg cctgatcgac gggatgaaaa aatccggtat tgaactggat
    54301 cgtaaaattc tggcggaact ggccgtcaat gatcctcagg gatttgcgca ggtcgtgacc
    54361 attgccaaag gcaagtaaac tactgctatg atgaaagagc ttcaacaact ggaaagcgat
    54421 gctttgtcgg aattaagtac cgccacgtcc gaggagcagg tcttgactgt caagacgaaa
    54481 tatctgggca gaaaaggcct tctaaccggt cttttaagaa acatctcccg ggtgcctgat
    54541 gaagaaaagc cgcttttcgg taaacgctgc aacgagttga aaaattctct gaatgccagg
    54601 cttgatcagg ccattgagca gtacacaagc cggaataaag aacaagcgct cttcaaggaa
    54661 aaaattgatg tcacgctgcc gggacgatcc gtcaactgcg gacgtgtgca tcctgtggtt
    54721 cagatccgca gagaaatctg ttccgttttc tcgtcattcg gtttttccat cgtggaagga
    54781 ccggaaatcg aactggatta ttataatttt gaagccctga atataccgaa agatcatccc
    54841 gcgcgggaca tgcaggatac cttttatatt gatgagaaca tcgttttaag gacgcacacg
    54901 tcgcctgttc agatcagggt catggaaaag atcaagccgc cgttgagaat cttatctccc
    54961 ggaaaagtat accgccgcga ttccgatgtg tcgcacacgc cgatgtttca tcagatcgaa
    55021 ggtctgctgg tggataaaaa cgtgaccttc ggcgatctga agggaatcct gtcggcgttt
    55081 ttgaagaaga tcttcggcag ttcaaccatg ctgcgtttcc gccccagttt tttcccgttt
    55141 acggaaccgt ctgcggaagt ggacatctgc tgtgtgatgt gcggcggcaa aggctgcagg
    55201 gtttgcggac aaagcggctg gctggaaatt ctcggatcgg gcatggtcga tccggcggtt
    55261 ttcagaaatg tcgattatga tccggacgaa tattccggat ttgcttttgg actggggctg
    55321 gaacgtattg ccatgttgaa atacggcatt tccgatatcc gtttattttt cgaaaacgat
    55381 atccgtttcc tcaaacaatt ttaggagatt ttttctttat gctggtcggt ctcacgtggc
    55441 ttaaagatta tgtcgatgtg caattaacgg cgcaggagct ggccgacaaa ctgacgatgg
    55501 ccgggctgga agtggacgaa ataaaaaaag tcggtccgtc cttcagcggc gttgtggtgg
    55561 caaaaatcat atcggtcggc ccgcatccgt ccgcagagcg tctgtctttg tgcgatgtgc
    55621 atgacggaaa ggacctttat cggatcgtct gtggagccaa aaatattgca ccgggaaata
    55681 tcgttccgct ggcgaaagtc ggcgcggtga ttccgggagg ctatacgatc aaatcctcgg
    55741 ttcttcgcgg tgaaaaatcg gacggcatgc tctgttccga agccgaactg gaaataggaa
    55801 gcgatgccac gggcattatg caactgtccc cggatcttgt actcggtcaa ccgttggaag
    55861 aggctctgaa ccttgaggat acggttttgg atgtcaacgt aacgcccaat cgtccggatt
    55921 gtctgagcat gatcggcatc gccagagaag tcgccgccct gaccggaaga aaaatcaaat
    55981 ggcctgctgt ccggattcac gaaaaggctc cggacatcca ttccctaacc tccgtaaaaa
    56041 ttatcgattc cgatctttgc ccgcgttaca ccgcccgcgt gatcaaaaat atcgccatcg
    56101 ctccgtcgcc cagatggatg aggcttcgtc tggagtccgc aggactgcgg tcgatcaaca
    56161 atatcgtgga tatcaccaat tttgtaatgc tggaaacggg tcagccgctg cacgcgtttg
    56221 atttccgttt tctggaagag ggaagaatcg ttgtccggcg gtcccttgaa aacgaagaat
    56281 ttatttcgct tgacggcaag atacgcacgc ttcccgccga taccgtttta atctgcgacg
    56341 gcatcaaacc ggtggccatt ggcggcatta tgggggggct gaattccgaa gtcaaagaag
    56401 acacgcaaac ggtgcttttg gaaagtgcgt attttcgtcc gacctcgatt cgttcttcct
    56461 cgagaaaact gggcatgtcg acggatgctg catttcgttt tgaacgggga atcgatcccg
    56521 acggtgtggt cagagccctt gaccgtgccg cccagcttat ggtcgatctg tccggaggtt
    56581 ctatctgtaa aaattatatc gatgaatatc ctcggaaaat ttcttcagcc ggagatattc
    56641 ctttgcggct cgacaggatc cgcaaaatta tcggcacaaa aatcgccgcc cgggatgttt
    56701 tgcaaatatt gcggcggctc gacatgacgg tgaaaccgga agggaagggc agatatctgg
    56761 tgacgcctcc gacctgcagg gtagatcttg aaagagaaat cgatctgatt gaggaaatcg
    56821 tcagactcta cggttacgat cgagtacctg ttacgctgcc cgacgtatcc gtcaacgaac
    56881 tggacgttat tgcccgtctg gatatggaag aaagacttcg tcaactgttg acgggaagtg
    56941 ggtttacgga aattgtcaat tatagtttcg gtacaccctc tgccgcggat atgctttccc
    57001 tgcccgcaac agatgaaaga cgaaatacag tcagaatcaa aaatccgctg ggagaagagc
    57061 tctccacgat gcggacaaat ctgatttacg ggttgctgga tacggccaaa agaaatttca
    57121 ataacggcgc attcgatttg aaactttttg aaatcggccg gatatttttg catcgacggg
    57181 aaggggaatt gcccgaagag aaaaccatga ttgcgggact catcacggga aagatcagag
    57241 atgatttctg gaactccgaa cagacggtgg acttttatac tttaaaagga gctctggaaa
    57301 atatcttttg tgacttcaag attcagaact gccgctacgc agcaacgacg gacgagtcct
    57361 ttttgcatcc gggaaaagcc tgtcacatag ttttatatga aaccgtaatc ggtgctgtcg
    57421 gagaaataca tccggatgtt ctggaaaaac tggatatccg caatacaatt tatgtatttg
    57481 aaatgaatat cgatattctc agcagggttt gtgagcaaaa cagcctatcc tttaacgaag
    57541 tgtcgaaatt tccggctgtt atccgcgatg ctgcgtttgt cattcccgat ccgatggaag
    57601 cagagcaaat gatagacatt gtcttgagcc agatggaaga attgcttgaa aatgtaagta
    57661 tttttgatgt atataagggc aaaggaattc cggaaggaac aaagagtctg ggattgcgat
    57721 tttcttaccg cgccaacgac agaacattga ctgacgccga agcaagtgcc gttcacgaca
    57781 gaatcgtgca gaaaaccgtc aatgtgaccg gtgcaaaact aagatcataa aatattcact
    57841 aagtaaataa aatggaggta aaatatgacg aagattgata ttattcaaaa tgttcatgag
    57901 aaactcggtt tttccaaaaa agattcttca gatatcgttg aagcggtttt tgatcttatc
    57961 aaggattgtc tggccaatgg cgaaaaagtc aagatatccg gcttcggcaa ttttatggtc
    58021 aaggagaaga gagcccgccg cggacgtaat cctcagacag ggcaggaaat ttccattacg
    58081 gcaagaaggg tgctgacgtt caaatcctca caggtgctgc gcaaagccct gaattaaatt
    58141 tggacattag ccggtccggt tgatttcata agcacggcgt atttgaaata attttttggt
    58201 tttacttcag ccttaaagac tttgagaacg ttatggaaac gatcattccc gataaagctt
    58261 attttcgcat cggcgaagta agcaaaattc tcaatgtgga accctatgtc atccggtact
    58321 gggagactga attcaaaaca gtcaatccca tgagaacgaa aaccgctcaa aggctgtaca
    58381 gaaaaaagga tgtgcaggaa ttgctgacca ttaaaaatct tctctacgca caacgcttca
    58441 ccattgacgg cgcaaaaaaa cagcttcaaa aattacgcaa tacggatgag cctgaagaga
    58501 aaaacagcga aaaacaaaaa ctcttcttga tacggatcaa acaggaatta caacacatca
    58561 gggagattgt cagttaatat aaaaaagccg gctttcaaaa gccggctttt ttcatctttt
    58621 cgtttttaag cttttttgct ggcgcgtttg gaggccttga gcggattaat cttttgagct
    58681 tctttgattt caatttttac gtgcgtagct gtcgggcacc ttccaatcag ccacttccct
    58741 gcaggatcgg gataaacctt acaatatctg gccgattcat attcaaatat ttttccgcaa
    58801 ccttcacatt gttcaacaac aacctgacaa ctgccgttgg tataactgca gccgttttta
    58861 gtcataaaaa cgcagtcaaa gcccgcttta accgtcttgc aaatcatgga tgcatccccc
    58921 ttatataatt cgagtatttg acgttacggg gaaaaactat caaggatatt ttcaccaacg
    58981 tctcggttat ttaaagtttg tgtccttaac aacaaaaatt atagctgtca agtaaaatga
    59041 atttcctgaa ataaaaagaa gaagggtttt ctttccagaa ataacttgaa tgaataacga
    59101 tttattgata aatatataaa aattgtttac ggaagagtca atgcgagcag tcattcagag
    59161 agttgaccgc gcctgtgtca gcattaatcg tcaggtattc tcctccatca acgaaggaat
    59221 tcttatatta ctgggtgttg agaaatcgga caccgaggcg gatgccgatt atattctgga
    59281 aaaagccgtc aacctcagaa tttttgaaga tgatcaggac aaaatgaacc tctctcttct
    59341 cgacacccaa ggagacatga tggttgtctc gcagttcacc ttgctgggga attgcgtcaa
    59401 aggaaggcgg ccttcgtttg acgctgcgga agaacctgtt caagcctgca ggctctatga
    59461 ctattttgtt cgtcaggcga aaacattcat tcccggtctg gccacgggaa aatttcagga
    59521 aatgatgaaa atagacctgg tcaacgatgg tccggtgacc atcatactgg acagcagaaa
    59581 gatgttttaa aaatggataa tttcgaaata agaatagaca aagacggtct ctggtattat
    59641 aaaggcgcgc acatgtttcg caaggatatt ttgagtatct tttacgaaca tctccggatt
    59701 gatgattcgg gccggtatct gattaagctc ggtccggaat gttgttatct ggccgtggaa
    59761 gatacggtgt ttgttgtcct ggccgttttc aaaacaaaaa atgcggacaa ccgccgggat
    59821 cagatcgaaa ttctgttaaa cgatgatacg tgcgaaatcc tcgacatgaa cagtctgcgg
    59881 tcaggcaagg aaaacgtgct gtactgccgt gttaagatcg gaaaattcac cgcccgattc
    59941 tcgcgcaagg cgtattatca actggctgaa ttcatcgaat catcggaaga cggcagccgt
    60001 ttctttattc ccctgaacgg tcaaaaatat tttatccgtc aagttcagga ttcggacgcc
    60061 tgcccgacaa ggccatcctg atgattaaat ggtgctgaga cagacggacc atgatgacgc
    60121 cacaggcatt ctgcagcgtt acggggaata aaattttctc acaagagcgt tgaggctcta
    60181 tattattaac aagcaaccgg gatttattat caatggaggg aaaaatgctc gatgacacaa
    60241 ttaaggaatc tttaacgttt gacgatgttc tgctggtgcc ggcagcgtcc aatatactgc
    60301 cgaaagatgt cgatacaacc acatcgttaa ccaaaaacat atctttaaaa attcccatcg
    60361 tttcggcggc catggacacg gtgaccgaat cgcgcacagc aatttgcatg gcccgtgaag
    60421 gcggcatcgg catcatccac cggaatatga gtattgaaca gcaggccatt gaagtggata
    60481 aagtcaagaa atcggaaagc ggcatgattg tcgatcccat caccattgat ccggaccgga
    60541 aagtgagcga tgctttgaaa ctcatgaatc agtattcgat ttccggcgtc ccggtcgtga
    60601 agaaaggaaa actggtaggc atcctcacca atcgggatct ccgttttgaa gaaaatctgg
    60661 atcaaccggt atcgaatgtc atgacgaaaa aaaatcttgt cacggtatcg tccaatattt
    60721 ctctggaaaa atccaaaaaa cttctgcatg aacaccgcat cgagaaactg ctggttgttg
    60781 atgatgaata ccgattgaag ggattgatca ccatcaagga cattgaaaag atcagacgct
    60841 atcccaatgc ctgtaaagat tcgctcggcc gattaagggt cggtgccgca gtagggatca
    60901 tcgatcgcga agccagagtt gaagctttgc tggccgcagg cgtcgacgtt attgcgattg
    60961 acacgtcgca cggacattcc gcaggtgtca tcaaggccgt gaaagcgacc aaatccgatt
    61021 ttcccaaatg tgaactgatc gccggtaatg tggcgacggc ggacggggcg aaggcattga
    61081 tggatgccgg cgtggacgct gtgaaagtcg gcgttggacc ggggtccatc tgcaccacgc
    61141 gtatcattgc cggagtgggt gttgcacaga tgtctgcaat cagagacgcc tacaaaatag
    61201 cctcgaaaaa aggggttccg gtcattgccg atggcggcat caaatattcc ggagatatcg
    61261 tgaaagccat cggtgccggc gctcactgtg tcatgatcgg cggattattt gccggcacgg
    61321 aggaaagtcc tggcgaaacc attctgtttc agggaagaag ttataaagtg taccggggca
    61381 tgggatcgct tgaagccatg aaaatgggta gtcgtgaccg ctattatctc gacgatattg
    61441 aagcgccctt aaaaacggtt ccggaaggca tagaagggcg tgtgccgttc cgcggttcct
    61501 tatcggcgag tattcttcag ttgatcggcg gcctgaaagc gggcatggga tacgtcggtt
    61561 gcagaaccat tccggacatg attaaaaaag cgcgttttgt aaaaattacg caggccggtc
    61621 ttagagaaag ccatgtccat gacgtgatca tcactaagga agcgcccaac tactggttgg
    61681 attaagtcag ttgctttctg ctttttttat tttttcatta tttcccaagt acggcaatta
    61741 tgaaacactc tcagtttgtg catcttcatc ttcatacgca atacagtctt ctggacggga
    61801 tgatccgttt ggatgagctt ttcaaaaaag ttaaagcata tgccatgccc gccgttgcca
    61861 ttacggatca cgggaacatg ttcggagcga tagattttta tcaacaggcg tttaaaaacg
    61921 ggattaaacc catcatcggc actgaacttt acgtcgcccc gaaagaccgt aaagacaaaa
    61981 ccccgtcgtc catcggcgaa tcaacgcgcc atctggttgt tctagtcaaa aacaagcagg
    62041 ggtataaaaa cctgattaaa ttaaactcgc tcgcacatat tgaaggattt tactaccgtc
    62101 cccgcgtgga taaggccctg ctgcgggaat gtgcggacgg cctgattgct tcgagcgcct
    62161 gcctgcatgg agagatcgcc tcatggcttg tccggggaaa catggaagag gcgagaagag
    62221 ccgcccggga atacctggaa attttcggag aagagaattt ttatctggaa atcatggaaa
    62281 acggaattcc cgagcaaaag atcgccaatc aggggctgat tgagctgagc aaggaacttt
    62341 ccattccgtt gattgccacc aatgactgtc attatctgga acgggaccat gcggaagccc
    62401 acaatgtgct tttatgcgtc caaaccggca aaaccattga agacaaagat cgcatgacca
    62461 tggcaacgga tcagttttat gtccgatcac cggaggaaat gtgcgagctt ttcgagtctg
    62521 ttcccgaagc gctggaaaac acggtcaaaa tagccgagcg atgcaacatg actttcgaat
    62581 tcgggaaatt ctatctgccg aaatttgaag tgaagcatcc ggaggaaacc ctggaggctt
    62641 atctggagcg caaggccgtt gaaggtcttg aaaaacgcat gccgagcatc ctgaaatatt
    62701 caacgggaga ggaaaacagc atccgcaaaa aatatagcaa gcgtctccac gaagagctgg
    62761 aaatcatcaa aaaaatgggg tttgccggat attttctcat cgtttctgat tttattaaac
    62821 atgccaagca caacgatatt cccgtcggtc cgggccgggg gtctgcggca ggatctctcg
    62881 tggcttttgc catccggatt acggatatcg atcctcttcg gtatggccta ttctttgaac
    62941 gattcctgaa tccggaccgc atcagtatgc cggatatcga tattgatttc tgtcaggaac
    63001 gccggggcga agttataaaa tatgtgacgg aaaaatacgg aaaggacaag gtaacccaga
    63061 tatgcacatt cggtaaaatg ctggccaaag gtgtgatccg ggatgtcggc cgtgccttga
    63121 acattcctta cggcgacgca gaccggattg ccaaactggt tccctctatt ccgcccaaca
    63181 ttaccctgaa cgaagccttc aaaatggagc cccgttttga ggaagaacgg aaaaagaatc
    63241 cgcaaatcga caaactcttg aaattatcac tcattctgga aggcttgaac cgtcattctt
    63301 ccgtacatgc cgcaggtgtg gtgatctcgg acgctccgct gatggaacga gtcccactct
    63361 tttccccgaa ggacgatatc gtttctcaat acaccatgaa agacattgaa acggtcgggc
    63421 tgacgaaatt cgatttcctg ggcttgaaaa cattaaccgt tatcaagaac actctccagt
    63481 tcatccgcga ctataaagac acggaggtgg atattaacaa cctgccgctg gatgatcccg
    63541 acacatacaa actgctcatg aagggagaaa ccgacggtgt ttttcagctg gaaagcgagg
    63601 ggatgaagga tctgctgatc aatttcaaac ccgatcgcat cgaagacgtg atcgcgctga
    63661 ttgccgcgta tcgtccgggt cccatgaaga tgatcccgga ttttattgcc cgcaagcacg
    63721 gcaaacagca aatcacatat gagcttccgc agcttgagcc gatcctgaaa gaaacgtacg
    63781 gcattattct ttatcaggag caggtcatgc agattgccaa tgcgatcggc ggttacacga
    63841 tgtcacaggc ggataccctg cgcaaggtga tgggaaagaa aaaagtcgcg gatatggaaa
    63901 aagaaaaacc gcggtttatg gaaggcgcca aaaaacagaa gatcgacgat aacaaggcca
    63961 gaattatctg ggatcagatg gaaaaattcg ccgaatacgg ttttaataaa tcccacagcg
    64021 ccgcctatgc aatgatcacg tatcagacag cctatctcaa ggcccattat ccggtcgaat
    64081 ttatggccgc tttgttgacg agcgaaaaag acaaccggga caaaatcatt aaacacatga
    64141 cgagctgcaa ggacatgggc atcaacatcc tgccgcctga cattaatgaa tcggaaaagg
    64201 ggttcagtat ttccggtgag aatattcgct tcggtctgac cgccgttaag aatgtcgggg
    64261 aggcggcgat tgaatctgtc atcgacgtgc gagaaacagc aaaattccta tcctttattg
    64321 attttctcaa tcgtgtcgat ctgcgtaaag tcaacaagcg ggtcattgaa agcctgatca
    64381 aatgcggcgc attcgattcc atgggacata aacgcagtca attaatgcat agttatgaag
    64441 aagcgatgga ggttgcccaa cgcaaccaga aggagaaatc aagcaaccag tcaagtttct
    64501 ttgatcagct ggaatccggc tcatcatctt ttgaaaacgg attaagcgct tatgaacttc
    64561 ctgacatgcc ggaatgggat cacaaaaagc tgctgtccct cgaaagagaa gcgctgggtt
    64621 tttatatcac gggacaccca ttgcttccgt atactgatcg tcttaaactg gttacagaca
    64681 gcgattcgga aaatctgctg atccgtcgcg acaaggaaac cgtgaccgtt gccggagtgg
    64741 tcagcgccat aaacgaacgg aaaacaaaac gaaacgacac catgtcgtat gtcactctgg
    64801 aagatttgca tggatcaata aacgctatat tttttggtga tttatacaag acatcctata
    64861 atattcttca tgaagaagaa ccggtgattg ttaaaggaac tcttgatata tccggcactg
    64921 aagaaaacca gaaaatttct ttaattgccc aagaagtgac tcccctggcg gaagccttga
    64981 aaaatcccta caaacaggtt cgttttatgg ttgacgccga caggatgtcc gctgaagata
    65041 tttcatcctt tatctcttca attcggaaat ttcagggaaa atatgaaagt tatatgcata
    65101 ttatgaacgg aaaaagtgaa acgatcgttt atctgggaga tgatttgcgc ctggatatta
    65161 atgagaggtt gaaaaaagag gcggataata tactggggca gggtgctaca attttttcat
    65221 gaggggattg acgtcagatg ccgccatacg aggagagaac atacagatcg ctcataaaga
    65281 acgatcatct catttcttat aatgtaaaaa tttccgaaag cgatctgttc atccgatccg
    65341 acaccgatct ttcagatcag gccttccaat cgcttgccaa tcatcgtcac tctttacaaa
    65401 tctatatcca aaatcattcc gatttcagaa cgtctctttt gccggttgat gaagaccatc
    65461 ttgcccctgc aattgtccgg gatatgatcg ataaatctaa aatatgcggc gttggtccga
    65521 tggcgggcgt agcaggcgcg atatccgaat ttgtcggacg tgacctttta agctctacaa
    65581 aaaacatcat tattgaaaat ggcggggata tttttctgca atctcaggat acattgaccg
    65641 tatcaatttt tgccggagaa tctcctttaa gccataaagt tcatttagaa atcaagcccg
    65701 aagaaacgcc tgtgggaatt tgcacatcat ccgcttcggt gggaccttca ttaagtttcg
    65761 gaagtgctga tgccgtgtgc gttctttctc catcagccac cctggctgat tcggcagctt
    65821 ctgcgatagg caacagggta aaaaacaact ctgatattaa gtccgctttg gatttcggaa
    65881 tgaatatccc cggtatcagg ggaattgtca tcatctgcaa aaaagaaatg ggtatcatag
    65941 gatccgttcg acttctttag ataaaacatc acaactcatt ataattctat agtcattccg
    66001 gcgttcaaaa atttatttta gaagaagggt taaagtgatt caatgggatc agagactttt
    66061 tttaatcgac cggatggttg tatatttccc cataccgtta tgtttacata acatatctta
    66121 taagacataa taattttgat taaatagccg cataatccaa gtctctatat ttatcgtgat
    66181 tgatttagct atgattatta gatgttttat tcggtgtggc ttttataatc catcatcggc
    66241 gagttgctgt ataattttta tttgacggtc cgtcagtcta cggggaacat caacatttat
    66301 ttttacgaac tgatcgccct tcaatgctcc ctttaatccg ggcacaccga atcctttcat
    66361 ccggatcttc gtattatttt gagtaccggg agcgatcttg atccgcttgg ttgttccgtc
    66421 tagggtggga acatcgatgg tcgttccaag agcggcttga gtaaatttaa tcgttttttc
    66481 gatatggaga tcgttgccgt cgcgggcaaa aattggatgc ggcagaacat taatattcaa
    66541 atataagtca ccgctgggac caccattaaa gccggacaga cctttgccgg gcagccttaa
    66601 ttttttaccg gcgctgattc ctgctggaat tttgacatta atatcttcta tgcgattttc
    66661 cagttgaaaa gaaatctttt tttccgcccc taaaacggat tcttctaacg taattgataa
    66721 gttatattcg gcatcctgtc ctttttgcgg catatttgcg taacgtgctc ggtttccgcc
    66781 cattgcaccg ccgaacaggc cggcaaaagg atcgtcatat tctccttcgt acccccccct
    66841 ttttctgttt gttctgaagg ttgccctgcc ccctttgata cctccaaagc cgaagcttct
    66901 gagtatttca tcaagatcga aattacggaa aatatcctcc tgcgaatacc gctgtcggaa
    66961 ttgatcggcc gaaccgaatt gatcgtattg attgcgtttt tctggatcgc tcagaaccgc
    67021 ataagcttca ctgatttttt tgaatttttc ctccgccgtt ttgttattgg ggttcttgtc
    67081 cggatgccat ttaagagcca gttttctata ggctttctta atatcatcag ctgatgcctt
    67141 tttttccaca cccaggacct gataataatc ttccgccata tgttttacct cttattattt
    67201 aaatatattt gatgaaagca tcaggtttta tattaagcat gaattcccat ttgaaaagct
    67261 tccagattca cttttattaa cttttccgga aacgactttt taatgacacg ttcccaaatg
    67321 gatttgtcga tattcatgtg gttggataag gcgcccagaa ggataacatt ggccgttttt
    67381 atgttaccag ccttctgggc cagttttgac gcgtcaaaac tgatgaccgt tttgtaatta
    67441 ttttgcaaaa attcaacggc atcatcagga taagggctct cacccatgtt gacggcagga
    67501 ggatagactt cctccgtatt cacgacaatg gcggcgtctt ttctgagaaa cgaaagatat
    67561 cgcagagatt ccattttctc aaaagataca agtgtctgta tgcttccggg ctccgcaagc
    67621 ggagaataga ccttcttccc gtaccggacg tggctcgtta cgcatcctcc gcgttgggcc
    67681 atgccgtgaa cctcgctttt ttttacgtca aaaccggctt ccatgatgac ttcacagata
    67741 atgtcgcttg cccgcaggat accctgcccg ccgactccgg cgaataatat gttttttact
    67801 tcattttcag acatttagat aattccttta aattaactta tagcgttgaa tttacaaacc
    67861 tgtgcgcata atccgcaacc gttgcataat gttgcatcaa tataagaaac gccctttcgc
    67921 ttggagcctt ttttagttgc cccttttttc caggaaatcg gcggacaatt cagcccgatg
    67981 cagacccggc atccttcaca tttgtccggg tcgactttca aggccggttt gggttttata
    68041 tggcttctct tgaatagcgc gcagggtcgt cttgcaataa tcacagaagg acccggtttt
    68101 gtcagctctt cttttatggc ggacgttgtt gctttctgat cgatgggatc gacaaccctg
    68161 acatgttcaa tgcccagaac ggaaaccaga ttttccagct tcacttgctt tgtctctatc
    68221 cccatcagcg tatagccggt ccccggatgc tcctgcatac cggtcatggc cgtcgtgcta
    68281 ttgtccagga taatgatcaa cgcgtcactt tgattgtaag ccatatccaa aagcggggtt
    68341 attcccgaat gcagaaacgt ggaatcgccg atgacgccca caaccttgcc cttccctttt
    68401 tccttgagcg ccttgctcat gccgtgagcc ataccgatac tggcgcccat gcagatgcag
    68461 gaatgcagcc cctccaaagg tttcatgtaa gacagcgtgt agcagccgat atccccatga
    68521 acataaacct tatgtttttt taaggcataa aacaatccgc gatgcggaca acccggacaa
    68581 agattcggcg gtcgcgccgc caaaggcacc ttgtaatcgg acactttttt gatggattgc
    68641 ttttcaatcg catttcggat gactcccgga tcaaattcgc tggtataagg aaaaatttcc
    68701 ttgccggtga ctgctatgcc catggctttg atctgttctt ccagaaaagg atcgagctct
    68761 tcgataacgt atagtttttt aactttagaa gcaaaatcac gaatcatttt ttccggaagc
    68821 gggtaaacca tgcccagctt cagataagaa tatcccggaa aaacttcctt ggcgtaattg
    68881 taaggcattc cggcggtaat aatgcctgct tccgcattgg taatttcgac cttattttcc
    68941 ggaaacgttt cagcaaactc cttgagtgcc tgctggcgct tttccacctc ggctctccgg
    69001 attctggcac agtgcggaac catcacatat ttttccgcgt tatagacaaa tcccgtcttg
    69061 tcttcgtata cagccggttc ttccagagtg acaacggatt ttgagtgaga tacccgcgtg
    69121 gtggttcgaa agaaaaccgg cgtatcaaac cgcgcgctga tttcaaaagc cattttaatg
    69181 tagtgtttcg cttcagcggc atcggccggc tccagcatcg ggattttggc gaaacgggcg
    69241 taattgcggt tatcctgttc attctgggag ctgtgcaatg aaggatcatc ggcagtaaca
    69301 atcaccaggg ctccgttcgt tccggtataa ctgacggtaa aaacaggatc ggcggcgacg
    69361 ttgacgccaa catgcttcat gaccgccatt gccttttctc cggcgagggc cgcgccgatg
    69421 gcaacctcaa cggcaacctt ttcattggtc gaccactcgg cataaacccc gtcatattta
    69481 cagaaatttt ccagaatttc cgtactgggc gttcccggat aagctgttgc aaaccttatg
    69541 cctgcctcgt atgcgccgcg cgcaatggct tcattacctg acatcaatac tttcaataat
    69601 caaacctcca aatcaactga gagaagaaat tttatatttg atgaagacgg cttttgagga
    69661 atccgctgtt ttctccaggg ctttcttgta attttccagc gcattctttc tatcattcat
    69721 cagttcataa attcgggcga cattgctgaa ccccactgat tcaaatccga tgttgttgcc
    69781 cttttgcgct ttttgaaaat attcgagcgc ggatttgtaa tcttttctgg cttcgtaaca
    69841 atatccgatt gacgacagag cgagaaattt aattccggcg tggtcggaaa tccgggaaga
    69901 aacgaaattt ttgtggtggg cgatggcctt gtcatattca ccgaggctgt aataaatatt
    69961 ccccagccgg taatgcgcca tccttgcgct ccagctgtgc ggatatttct cgatcagttc
    70021 ctggaaaatc cggatattgt tcccggtcat atcctgtgtt tcacttccgg aagcgctcga
    70081 ttgcgtccgg gcatacatcg ctctggcgga gtcctgatag ttgctccagt acatatagat
    70141 accaaaggca atcattgatg ccagaatgac agccgtcaca atggagtaaa aacgcttttt
    70201 gttttcagaa atgtaatcct tgaccagttc cacggtgttt tgaaaagcat ccggtccttc
    70261 caaatcctgt tttgttaatt tctcagccat atgcctcctc tgtgttgata ttatattttt
    70321 tgaccaattc caaaagcaat tgaggtattc tgcgaagctt gccctcgtga ttggtgcaag
    70381 cgtgaaccgt atagccttcc gccagaagat gtttttgatc ttcatcccat atcttggaat
    70441 tgaaaccaac cgtcgctttc cggatatagg caaattctgt ttccaccgcc aaaagctgat
    70501 cgtatgtggc aaacttcagg tattcgcagc cgaccttaat caacggaagg ttcaaattca
    70561 actttgtcaa ttcgctgtaa ggaacaccca gctgccgcat cagttcgttt ctgccgagtt
    70621 caaaccactt gatgtagttc gtgtgataga ccacccccat ggcatccgta tcggcaaaaa
    70681 gcaccctgat ttttgttaca ttgttaaccg acaacgctct tccagtcctt gataaatttt
    70741 tccatcagca aatgccccgg agaaatcaga agattgccgg ctttggcgtt tatctcatta
    70801 aagctcattc ccggggccct gttctctttt ttttccgaag acaataccgc aaaagggctc
    70861 ggatcgctga catgggtcct gagatccaac ggtgtcggat gatcgcttaa aacaataatc
    70921 ctatagtggc cgaatgcgcc tattttcgtc aaaataggcc cgacgatttt ttcgtcaaaa
    70981 agttcaatcg cacggatttt gccttcggca ttgccttcgt gtcccatttc atcaggggct
    71041 tcaagatgga caaaaacaaa gtccatgaaa ttcaatacat ccagagccat ttttgcttta
    71101 ccttcgtaat tggtatcaat ataaccggtt gccccctgca ccggacaaac tttcaacccg
    71161 gcgatcgttc caatgccttt aagcagatca acggcggaaa tcatgcctcc cgatagggaa
    71221 tacttctgag tcagagggac aatccgcggc ttcttcccct gcccccacag ccagatggca
    71281 ttagcctctt ttttgcccgc ctggcgtctt ttcgcattga ccggatgatt ttttaaaagg
    71341 tcggccgccc gcctgataat ggcattgaca tcggcggcag catttccgcg cggcagatat
    71401 tcagccacgg gctttcccgt aatatcatgc ggcggtgttg tgtccggatt ggccggggcg
    71461 ttgcgccaga ccagcagatg acgatacccc acgccgggaa agaactccag atgatcggaa
    71521 ccgatgtgcc gatggatatc gcgaatgatt atttccgctt cttcgctgga gatgtgcccg
    71581 gcggtaaaat catccataaa cgcttccgga ccatcctgat cgccgatggt aaccagattg
    71641 cagcgatacg cgacatcatc tggaccgaga tcaattccca tgcttgccgc ttccagcggt
    71701 ccgcggccgg tataattttc ccggggatca taccccagaa cgctcaatgt ggccacatca
    71761 cttccgggat gaattccttc gggcaccgta tcgacaaggc ccagtgttcc ttctgcggca
    71821 atctgatcca tgaacggagt gaatgcgcat tccagcggtg tttttcctcc cagcttatcg
    71881 gtcggataat ccgccatacc atcacccagc aatattacgt acttcatatc acctttctga
    71941 gaaatctctt ttttgaataa tttaatttaa atcgtcctca attctgatca ggatggtttc
    72001 accctggagg atgtccagtt tgcttattct cttgagtgct ttctgcacat cactttcctt
    72061 ggctttgtac gttgtcatca caatgggcac cgctcccctt ttcactctgg ctttctgaat
    72121 gcaggcttca atgctgatat tgtatttccc gagaataccc gatatcctgg aaagcacgcc
    72181 gggacggtca accgcggtaa aacggaaata atattttgtc tccacatgat ccatgggaat
    72241 cagccggatt ggcatcatgg ccgactcgtt gatcgaccgc aagggccctc ggccgttttt
    72301 tccgctcaga acatctcttg cactgtccat aacatcgcta aaaaccgcgc tggcggtggg
    72361 catcatgccc gctccctgac cgaaaagaaa gacggggcct gaggcatcgc ccatgatatg
    72421 aaaggcattg taattctgat tcacatttgc cagaagacat tttgacggaa tcatcgtcgg
    72481 atgaattctc gcttctaccg cattgtttct gagcattgcg atggccagca atttgatccg
    72541 gtagcccagt tccttcgcaa acgaaatatc ttcacggctg attttggtga taccttccag
    72601 atatatatcc ttgagattca ctttcctgcc ataggccagt gagagaataa tggccatttt
    72661 gtgcgcggta tcgatccctt cgatatcaaa ggtcggatcg gcttctgcaa agcccagctt
    72721 ctgcgcctct ttcaaaacca tgtcgaacgg cttgtcgtca tccgtcatct tgctcaggat
    72781 gtagttgcag gtgccgttca tgatgcccat gacggaaatg attctgttgg caaccaggct
    72841 ttccttcaag gtcttgatga ttggaatggt tccgccgaca ctcgcttcaa aaccgatatt
    72901 gaccgcattt ttctgagccg ccgggaatat ttcattgccg tgggtcgcca gaagcgcttt
    72961 gttggcggta accacatgct tgccgttggc aacagcttcg agaacaaacg tgcgcgccgg
    73021 ctcgtacccg cccatcagtt caatgataat cggaatgtct ttatcgttga tgatctcccg
    73081 cgcatcccgc gtcaagagcc ccttatggac cctcacttca cggggtgtcg tgatatcaat
    73141 atcggcaatt ttctttataa caagcctggc gcccagtttt tttgtaatca gctcttcatt
    73201 ttgccgcaac agtttgacca cgcccgttcc gatggttccg aatccgataa gaccgatagg
    73261 gatttctttc attatatgca cctgaaatta ttttataatt ttatataatt cccgttaatt
    73321 gttaatcttt ttccgttaat ttcctataac aaatggatcg gctttgtcaa tgaaatatcc
    73381 atcatgaatg cttaaaataa ttaatgaatt acccagacat gaagtaaacc gattaaaaac
    73441 cggatatttt gcaaaagccc cattacttga ccgtgatcgg tttattttca acaccggcat
    73501 agaaaacaat aatctcggcc gcttccttgc cttcattttt cccgtaatgt ttcttgttga
    73561 ccagttccac aatggaatct ccggctctga gatgcaaagt tttgttatcg tccgccacaa
    73621 ccgtcaattc tcccttgagc aaaacgcccg cgttaatcac cggatgatga tgcatttcca
    73681 gttttgaacc ggcaggaatt ctgatgcgca gaattgtaac ctccggctga ccctggaggt
    73741 attgcggcag cgtttcgcca tcccagctcc tgactgtttt ggccagttgt accgtttcaa
    73801 tgctatgtaa acccgccgaa cacccggcaa acaaaaaaaa taaaaccgac ggtattaccc
    73861 agatgagctt tttcaatgtt tcctcctctc gttggattaa aaatcatata gggggattat
    73921 tctaattacc gcgcacatgg cgggcatacc atcgccgtcc cagccagcgt tgatggtgcc
    73981 aggcaaaaaa cggcaagatt cccctttttc tcaaccagta accttgggga atctctcttg
    74041 agtcctgttt tggcatcatt agaatttccg cactgacaac cccttcgcct tcttccggtt
    74101 tacggtgcgc aagtatccgc ccttccgcat caagaataac ggcattgcct tcgtaaaacc
    74161 ctgtatagct catgggcagt cccggcatgg cacattcaat ttccccgcaa tgtgcggcat
    74221 gaatcaccgg tacgcctaaa atacgcgcgt tatcccggag cacctccaac gcattgcgtt
    74281 tgttgtccgg ttcccagagt ttctgcaaaa aaagcggaaa attagtcggt atgctccacc
    74341 agcatgatcc gccgatgacc acatccacct tgttttgcag ccggcgggcc gtcatcgttc
    74401 gcataaattc ccagcagacc gccgcgccga ctctgacatt ttcgtataag ccgagcacgc
    74461 cgatatcccc gggatcgccg ccttcataaa aataattttc ccacatggtt ggaatatctt
    74521 tatcatgctg accgacgagc cggccttgcg cataacattg ataccggttg cggacttgcc
    74581 cgtcgggcaa tcggcataaa aatgatcccc cgatcaaaac gcggtatttt tttgaaaagc
    74641 cgcaaagaaa tgttgccgcc tcgccgtccg cagattgaat agcccgggca atattcggat
    74701 tccagctgac gcccgtattg aaaaattcag gcaaggcaat ccattgtgct ccttcgttta
    74761 ccgcctgcag ggaaaggcgc tcacagcgcc ttatgttttc ctctgattcg gccacggcag
    74821 tggccagttg aatggctgca actttaattg tcatgggttt attccttcgg ctctttgatt
    74881 gattttaatt gcgcataaac gtcatcatgt aacggcgtag gcacgccata atcccgaccg
    74941 gccccgcaaa gataagccgt cagtgcgtca atttccgtct gccggccttt ttcgaaatcc
    75001 agctgcatgg atgttctgga atgatatccg aacccggcaa tcatcgccat cgctttatcc
    75061 acttcatgct cctgtataac gacatgatgt gcggcggcga cggcaaccac ctccatcatc
    75121 atcgcccttg ctttttgccg taattcagga ttttcccata tctgcccgta tgttttgccg
    75181 gtggccgatg tcagagaccc cagcggacac attaacaaat attttatcca gagcacgccg
    75241 gaaatgtcgt ccgttaatac cgcgttgatc tttgcctgaa gtaaaatatc aaggatatat
    75301 cgataatcgt ctgccggatc atgatcggtt ccgaacgtca gcttgcacgc gcctccttcc
    75361 tgcttaatga ttcccggtcc gtcactatgg gaaatgatat aaacgctgcc gcctaagact
    75421 atcgcatgcg gcaggacacg gcgcagccgt tgcgagctgt taacaccgtt gagcaacgga
    75481 atcaccacgg tttgtctgcc gatgcaggag atgaacgctt cagccgaatt ttccaatgaa
    75541 taacttttgg tgcaaaagaa caccacatca aacattcccg cctcatcggg attgtttgtg
    75601 gctattctcg gccggacaac acaatcgcct tcacgcgtaa aaagttgcag gccgtttttt
    75661 tgaatcgctt ttaaatgttc cccgcgggcg ataaatatga tttcatattc gtccgatttt
    75721 tcgtactcac gcgccagttt cccgccgaaa tatccgccta ccccgccgat accaatgatg
    75781 cctattctca cgcccatcat gcagctccat ccgtttgaaa cgccttttca ttaaaattat
    75841 cctgcattgt tattgaagtc aagcttcatc atattgatcg tcagcttgct tttatgatct
    75901 tcgatgtggt aggttactct tcacaaaaaa accggatatc aacgcaataa aaagcaaact
    75961 gcaaataagg agagcttcat gaggattcaa tcttttgtcg ttcatttgcg ttttaaatcg
    76021 gcaattcttt tcgcatcgat cttatgcata gtcatcagcg gctgcacgcc tttgctgaac
    76081 gtctcctaca aaaaatccct gcctcccatg gaaggagaac aggttcttcc cggtttgtcc
    76141 gataacgtca ctgtcaaacg cgacaatctg ggcattcctt acattgaagc gcagagcatg
    76201 gacgatctta tctttgccat gggatatatc agcgcttgtg accgctttac acagatggaa
    76261 ggattcaggc ttgtgagcca gggaaggctc tccgaacttc tgggcagggc cacgctggaa
    76321 atggatattt atctgcgggc gctgaatctc aatcaggtcg cccagaccct ttacctgtcg
    76381 gcatcctccg acctgagacg actgttaaat atctacagcg acggcgtaaa cgcgtatctc
    76441 gccaacgtcc ctctgcccac gactctgaag ttggccggac accaacccga tccctggaga
    76501 cccatcgact ccatgtatgt atttcttgtt ctaacgctgg gcctgagtca aaatctccag
    76561 gaagaaatca acctgctcaa cgtggcgcag gttgtcgcac cggaaaagtt agcctggctt
    76621 tttcccattt accccgatga acccctgccc tttgaagaaa tgaaaaaact gaaaggaatc
    76681 aatctttcag aggctgctca aaatattgaa caactgggcg tcatagctaa tttgaccaac
    76741 acactgcttc ttcctgccgc ggcggcttcc aataactggg cggtctccgg aaaactgacc
    76801 aaatcaggga aacccatcgt ggccaatgat acgcatctgc cgttgaccat gccctccatc
    76861 tggcacatga tgcatctgaa atgtccggag attgacggag ccggtattgg catcgccggt
    76921 gttcccggca ttgttgccgg atacaacagg cacatcggta tcggcatgac gatggtcatg
    76981 gcggacaatc aggatatctt tctggaaaag ctgcaacaga aaccggacgg tctctatttt
    77041 ctttataaga ataaatggtt gaaggcaaac agcagggagg aaagcttccg gattaaagga
    77101 caaaaaaagg ctgttacccg aaccttttat gaaactctcc acggcgtttt gctcaatgat
    77161 attttgaccg ataagccccg tcatgaactg gttcccatgc cgcttaatcc ttcattggga
    77221 atcgcgtata gttgggcagg caatgaaacg gacaggtcca tggacgtttt tttcaacatc
    77281 atgctcgcaa aatcggtcga tgagattctt aaatacactt ctcaggaaac gtccgtcatt
    77341 ccactgaacc ttgtcatggc ggatcgtaaa aatatcgcct ggcaggttac gggccgtttc
    77401 ccgttgcgga aaaaaggtcg cggtctctgt ccctctcccg gttggacggg tgaatacgat
    77461 tggaacggat ttctttcacc gtcaaaacat ccgtctttga aaaaccccgc gaagggctat
    77521 gtgggcaccg ctaatcatcg gacagttcct gctgattttc cttatattct gtcctcgtcc
    77581 tggtactatc ccgacagagg cgaacgtatc gaacagctgc tgaaaaatgc aaaaaattac
    77641 accctggatg atgcaaaagc tatgcagctt gatacctatt ccaggtttgt caacgtaatc
    77701 aaaacggcgt tgctggaaca aaaaacgctg gacaccatga gttctgtctg gcaaaactct
    77761 gaaaaatcaa cggcggctca tcaagcgatg gcactgctca gagattttga cggaaatatg
    77821 cgcctcgatt ccgccggggc ggctttctgc ggcgcttttc tgttttcgct gagcgacaat
    77881 cttttcgctg acgaactggg agggaaaaat acacgtgcct atcaatcact gctgcatacc
    77941 ttcctgctgg cctattccgc actgcacgac catttgacgg acagagggaa aagaagcccc
    78001 ctctgggatg acgtaaccac acctcagaaa gaagaacgcg acctgattct ggccaaaacg
    78061 gttctggatg ccatcggcat tctggaaaag cgctgcggaa aaaatcccga caagtggcaa
    78121 tggggaaaac tgcataccta tcattggagg actgatgcca cattgtttgc cgattatatg
    78181 gatttcatca acaagaccgg cgtcaaattc ctctccggtt atgtagacag aggtccctat
    78241 ccggcgcccg gcgaccacac cacgctgaac gtttcagctt atcatcccgg gaaagatttc
    78301 ggaacctgga tgattccggc catgcggatc attgtagatt tcggaagcga tgaacctttc
    78361 gtcggcatta acagcacagg gcagttggat aatccgtcaa gcccgcacta cgatgacggc
    78421 atcacggcat tccggttagg acagtatcag aattttccgt ttaaggaaga aaatataaaa
    78481 acacagtaca cgaaaatact gacattggtg cccaagaaat gatttaaaaa aaaagaatat
    78541 gctttgattg ctttgagccg ccagacacat tattcatgat ataccaactt ggctaaagcg
    78601 cttaacagcc ccctaagttc agtcgagtct tttggactcg actatttatc tgtgtttgaa
    78661 tacagccaag tcaaaaagac ttggctgaac aaagtccacc tgaacccgca agcttatgat
    78721 tcccatccgg ggaatagaga aagaatttac ctgctgacat ggatttttca taaatcaaca
    78781 cagaaccgtc tttgctcaaa agcggccgct taggtcgggt cttcggcggg aatttataag
    78841 gattgggaat cagctctttc cctttcatca caacaatgta atgaatcccc ttgccctgtt
    78901 tctttgctta ttaccccgtt ctggccaaat ccagtttccc ctgccatttc cagattaaga
    78961 cctccttcag aatttgatgt tcaactctgt ccagaaacgg atcgcgcgca gccaaagcga
    79021 aagcgtcctc cttggcctcg ctcaaaatac gggcatcgcg aataatactg gcaatgcgga
    79081 aatccggcag cccggattgt ctcgttccca gaaaatcgcc gggaccgcgg atagcaagat
    79141 cttcttccgc aatggtaaat ccgtccgttg ttgtttccat cacttttaat cttttgcgcg
    79201 catctgctga acatttataa ttggccaaca gaatgcagct ggagagaata tcgcgccgcc
    79261 ctaccctgcc gcgtaactga tgcagttgcg aaagtccgaa gcgttcggca tgctcgataa
    79321 ccattaatga ggctctcgga acatcgatgc ccacttcgat gaccgttgtc gaaacgagaa
    79381 tatgaatttt gttctccata aattcccgca taatttcatc tttttccttt tccttcattt
    79441 tcccgtggat taatcccacg cggtaatccg gaaaaacgtc ctgctgcaaa tgcctggaca
    79501 tattggtggc gtctttcaaa tccagcgttt ccgactcttc caccagcgga taaacaatga
    79561 atgcctgatg ccctttcgcc agttccgcgc ggatggcctg ataaactttg tcccgtttgc
    79621 tttccggcaa aacaacagtg tgcacgggct ttttccccgg aggcatttca ttgatcacag
    79681 agacatccag atcgccgtat acggtcatcg ccagggttct cggaatcggg gttgccgtca
    79741 tgaccagcac gtcagcgttg atgcccttgg atcgcaatgt cgctctctga ataacgccga
    79801 aacggtgttg ctcgtcaatg acaaccagcc ccagtttatg aaaatcaaca ccttcctgaa
    79861 tcaaagcgtg agtgccgata atgatgcccg cctcgccgtt ttgaatccgg cgcagggttt
    79921 cgccccgtga tgcattggcc atgctgccgg taagcaaaac cgttttcaat cccaatggct
    79981 cagcccacaa acggatattt tcaaaatgct gtttggccag aatttccgtc ggcgccatta
    80041 tggccgcctg ataagaattc tcacaagcgg taatcatggc tgccattgaa acaatggtct
    80101 tgccgcttcc aacgtctccc tgaaggaggc ggttcatcgc cgtgtctttc gacatatcgt
    80161 tgaggatttc ttcaatgact cttttttgag cggcggttaa atcgaacttt aaactggaat
    80221 aaaatttatc cagcattgtg ccatcggttt tgaatgaaag gccttgatcc agaattcttc
    80281 ccgatttttt gacagccatg cccaactgaa aaaagaaaaa ttcatcgtaa atcaaacgcc
    80341 ggtgagcctc ggaacggctg gagatgaatt gatcaatgtc ctcctgactg ccgggaaaat
    80401 gaacagacca caacgcttca tggacattca gcaaatgtcg cttttcacat atactgtccg
    80461 gaatcggaga ggcaacatag cgggaataat tcatgagtgc ggcattcatg accttgcgat
    80521 aatatttctg ataaaggccc tccgtttcgg aatacacggg cacgattctt ttgaaattca
    80581 gaagattttc ttcatccttc tcgtcaagaa tttcataatc cggatgaatc atctgttttc
    80641 cgacgtaatc cggtcttacg tcgcccgtaa aaataaccct ggttcctttt ttgaaaacac
    80701 ccaggagata cgccatctgc cctttaaacc acttcgcggt cagattggcc gtgctgtcgc
    80761 tgatcaccgt ttccaggatt ttttttcttc cgtaatagcg atattgacag ctgatcacgt
    80821 tgcccacagc cgattggact ttttcggctt cgagacggtt gatttttctg atttccctgc
    80881 ggtcttcata agtccgcggc agaaagaaaa gcagatcttc cacggtggcg atttttttat
    80941 tggcgaagcg ggacgccatt ttcgggccca cacctttcaa atactgaacc ggcagattca
    81001 gtttttccag ggcttctttt aaatccgata tcttctcaat ggttttttga tcggcattcc
    81061 gttcgggaac ggatatgttc aaaaaatgga tgatggtttt gagtttttcg acgattttga
    81121 cggcgttttc aatttttctt ttttttaatt ccagcttttg ccggtcgtaa tcggaaaaga
    81181 tttcccgtaa tgctccgatc tcatgattaa tttcctcacg gccgaacgat gcggcaaaag
    81241 aatcaagttt cgacaaaagc gttatgagcg ttctgcccag atctttgatg ttggaaagat
    81301 ttttgtaatt atcgcggaaa gcaaagttca ggggttgatc aattttttgg agaatttcgt
    81361 ttaaggcatc catttaaatc tccacgccgc aaatctgtca aacctgaatc atttctgatg
    81421 aatccggata tttttgttaa caaatatatt ttatttgcgc aaggaatgat tgacttaagc
    81481 gttgaattac gttaaaaact caccccgaaa gaacttccat atgaaattta agatcactca
    81541 gaggagctat gacagacaat ataacctata aagatgccgg cgtggatatc gacatcgcca
    81601 acgaatttgt ggaacgaatt aaaacatccg tcaaaacaac ggcgcggaag gaggttgtct
    81661 ccggtatcgg cggattcggc ggccttttcc gtctaaattc cgttgacctg aaaaatccgg
    81721 tgctggtatc ttccaccgac ggtgtgggca ccaagctgcg tatcgcccac ctgctcgaca
    81781 aacacgatac catcggcatc gacctcgtcg cgatgtcggt aaatgacgtg gttgtgcagg
    81841 gagccgagcc gcttttcttt ctcgattatc tcgctaccgg aaaaatcgag ctcgaaaaaa
    81901 gcgtcagcat tatcgaggga attacggaag gatgccgaca ggcaggctgt acccttctgg
    81961 gcggcgaaac cgcggagatg cccggttttt atgctcccgg agaatacgat ctggctggat
    82021 tctgtgtcgg agtcgttgaa tcggacaaat taatcaccgg atcaaccatc agtgtcaacg
    82081 accgtattat cgggctggcg tccagcggac tgcacagcaa cggattttca ctggcgcgga
    82141 aggttcttct ggaaaaagga aagctaaacg tcaacgataa gcttcccgga ctggaaaaaa
    82201 ccgtcggtct ggaaatgctc gaaccgaccc gcatttatgt taaagccctg ctgaatctct
    82261 ttaaaaaatt caccctcaaa ggcctggtcc atgtcaccgg aggcggattc tacgaaaaca
    82321 ttccgagaat tatcccccag gcctgccgtg ccgtaatcgg caagggcagt tggcctgtcc
    82381 cgccgatttt caacatcatt cgtgaaatcg gcaacattga tgaaaaagaa atgttccgcg
    82441 tattcaacat gggcatcggt atgatgatta tcgtttccga caaagactgt ccggcggtgc
    82501 tcgaatatct ggaaattctc ggtgagaaag cgtacccgct cggcctgatt gaaatcaggg
    82561 aagataaaaa gccgcaggtc atcatttctg aaagctaggg aaagcaaatg gcggatgcat
    82621 tgaaactggg aattctgatt tcaggaaacg ggtccaacct gcaatccatt attgatcaca
    82681 tcgagcaggg gtctcttcgg gcggtcatcc aaattgtcat cagcaataac cctgatgctt
    82741 acggcatcat ccgggcaaaa aaacacggcc tccctttttg tgtgctgaaa aacagcgatt
    82801 ttaaaaataa agaggaattt gattctgaac ttatcaacac gttgataaaa catgacgtcg
    82861 agctggtaac gctggccggt tttatgcgta tcatctcgcc tcgatttctt caggcttttc
    82921 ccgggaggat catgaatatc catccggccc ttcttccttc ttttcccggt ctgcacggac
    82981 agcggcaggc cgtgacgtac ggcgtgaaag tttccggctg tacggttcat ttcgtggatg
    83041 agggcgtcga taccggcccg atcattatac aacgagccgt gccggtgcgc gatgatgaca
    83101 ccgaggacac cctggcggct cgaattttaa aggaagaaca taaaatctat ccgcaggcga
    83161 ttcagctttt tgccgacggc aaacttgaaa tccggggaag aaaagttttc attcacccat
    83221 agccgttttt ctccggtcta atgtcttttt tctgatgatc cggcaatgac gtttaaggac
    83281 agaatccatg tatgatctga ttgtcatagg cgatgatctt tcttcccatg tagccgcagc
    83341 gttctgcgcc atcaacggtt tgaagacggc cctcttctcc gaaaaaggaa ccgccggctc
    83401 atctgttgcc ggagatctca cgtttgacgg cacgatcacg ccggtgtccg ggtttgccga
    83461 aaaccagatc gcctatctgt cattaaaaaa tctgggaatt tatccggagg tgcgcctgct
    83521 gaatccggcc tatcagatta ttctcccatc gcaaaggata gactttttcc atgacaaaga
    83581 agaacttctg aaagagctcg ccagagagtt tcctcatctc gagggcgatc ttcgattatt
    83641 ctacgatgta gcagagaaaa attcaaacgc agccggacaa tggatttcag aacatcccgg
    83701 cattcaaccg aaaaaaatga aggatttttt cgattatctc aaactgattc cccgtttcgt
    83761 cgaaagcatg ttcaacacca tccgtttgaa acgtctgatg tcgcaaaacc catccttccg
    83821 gaaagttatt caggctcagc atgcccttct ttctttcaaa acggatccgt taaattcttt
    83881 tttttcatca tggcaatcct gcacgccgtt gcgcggcacc ttctcctttg ctcagggcaa
    83941 acaggacctg gtcgattcgc tgatccgtaa aataaaagcg tccgacggtc tttatttatc
    84001 cggatgcgat tgcctgtcga tccataaaga caaggtcatt gaactgaatt atacggacga
    84061 taacggcaac accgaaaaaa tcgaaagccg ccatttaatc gtcagcacaa aatggcaaaa
    84121 catcggccaa ttgttcgacc acaagaaaac tttcagtttt ggcgattttc ttcggccggc
    84181 aaaaacggcg tatttcccct tttccatttt tctgggcgtc aatccggcag ggctgcccga
    84241 aaagctggcc agacattcgg caattatctg caatccggcc agggagcttt atgcccacaa
    84301 caacctgatc attctggaat cgtcggcaaa agaagagagg atgaaggatc ccgctgcaaa
    84361 aattcttctg agcgccaccg ttttcctccc cgatcatcag gaaatctggt caaaggataa
    84421 cctgaagact atttccgaaa acatcatgaa tcaacttgac catttcttcc cgtttttaaa
    84481 agaaaacatt ttgtttttcg atccggacga atccatgaag ctatcgctta agctgcgagg
    84541 caccatcatc ccgcgctatc agatcaaaaa ttcttttttt acgggttttt ccgctaaagg
    84601 cagtaaaacc cggttcgcca acatttatct taccggagcg tccttactgg cggatgccgg
    84661 ttttgaagca gaaattcttt ccggactcaa taccgcgaat cttctgattt cacaaaggaa
    84721 gtaatttatg aaagacgttt tttccatttc caatctcggc aaaagaatga ttgtctctcc
    84781 ccttcaaacg ggcaagttta cccgggacgg caaaagagcc ctgtttaaca ctcatctgga
    84841 caactatcgt tcgctgaccg ataaaaaagg acaaccgctt tccgtcgagt tggccggccc
    84901 ccgcgaaaag atttttttta atcctgccga caccagagcg gctatcgtta cctgcggcgg
    84961 cctttgcccg ggtatcaacg acgtgatccg ctcgatcact acagcgctgt acttcaatta
    85021 tcacgtcaag aagattgttg gcgtcaaata cgggctccag ggattaaatc ctcattgcgg
    85081 gcatccactg atctctctgt cgccggatgt tgtgaaagac atcagcagct tcggaggcag
    85141 catcctgtcc acatcgcgcg gtccgcagga tgtgcaaatc atcgttgact atcttcacag
    85201 attaaacatc aacatcctgt tctgtatcgg cggtgacggc accatccgag cggcggaaaa
    85261 aattacagcg gaaattacca gacgaaattt taaaattgcc gtgatcggca ttcccaaaac
    85321 catagacaat gatctccatc tgattcagaa gtcgttcgga tttgataccg cgattgagaa
    85381 agcggccgat gcgattaaaa gcgcccacgt ggaagcccgg ggcatggtca acggcatcgg
    85441 gctgatcaaa attatgggac gccactccgg acacatcgca gcccacgcag cgctggcaca
    85501 aagcgatgcc aattttgtcc tcattcccga ggtgcctttt gatcttgaag gcaaaaacgg
    85561 ctttttgggt tttttaaaaa aacgcatgat ttccagaggg cacgctgtca tcattgtcgc
    85621 cgaaggcgcc ggtcaggatt tgctcggcac aacggaccgc tccgcagaaa aagatgcttc
    85681 aggcaatgct cgtctgctcg atatcggatc gcatctgaaa aacgtcatcg aaaattattt
    85741 taaaaaaatc agcatggaga tcaatctgaa atatattgag ccaagctatc tgattcgttc
    85801 ggttccggca aacgcttcgg atgacattta ctgcaatatg ctgggacaat acgccgtgca
    85861 cgcgggaatg gccggaaaaa ccggaatcct ggtcggtttg atgaaggatg agtatgttca
    85921 tcttccgttg gccgcagtcg cctccggcag aaaaatcgat ccccgcgggg atatctggtt
    85981 aagggttctg gagacgaccg gccagccggc ctcgatgaaa aattaatcga atattaataa
    86041 tatcggatag ttaagaaaat attttcatcg tctttcagcc ggataatttg ttttttattc
    86101 acaacaaagg acgattgaca tttatctttc atgattggcg aaaaaatgct tgcaaaatac
    86161 tgtcttatgg tttagtaacg acgcacattt tctggaggat tgtaaaaatg tctcgtgttt
    86221 gtgaagtatg cggcaaaaag ccgtctgtcg gtaatgccgt gagccatgca aataataaaa
    86281 agaaggcggt ctggtatccc aacctgcaga acctgcgttg tgtagatgaa aaaaccggat
    86341 caataaagag aatcaaggtt tgcacacgtt gtattcgctc aggatttgtc aagaaagcta
    86401 cctgataaaa gaaattattt gagattcttt tattacatcg gctgtttcat tgaaaaacag
    86461 ctcatcatcg tcaatcaaat ccatcgcctg ctcaaacaaa cggttcaatt cattattaag
    86521 caatagatca ttgcagggaa ggccgaattt atcttgataa agatgcattc ggccttggat
    86581 ggtcatcatt tgctccatcg attcttgcgt gatgaacaac agggcgcttc tcatcacctc
    86641 tcgaatctga tttacttcca ggccgtagag ccatcgcagg gtttctgtaa attcttccat
    86701 cctgttttca accgtgtatt ttatggcgtc gaccatcacc tcaacagtct taaacgtgcg
    86761 gagatgcctt acgattacca gatcgtatat catttcgatg atcaaatggg actgataaac
    86821 ggcgtcgcga cacgaaattt ctttgcccgc aatgccatgt atatcctgta tgactcggat
    86881 aagaggattg cctttccggt aggaatagcc ccgggaatgg atatcgaatg catcgccgtc
    86941 ttcggtacag atatatccgt aatgcgaaag atcatcaatc agcagatgat agatgacata
    87001 ggccgctttc ggatactcaa caggaagggt ttgcagtctt cttaatttat gcgttctggc
    87061 ggaggttata tccggatgaa tcgccagcaa atccggggcg atattgtata caaaaatatc
    87121 cggttccaca ttttttatct ccgcggaacc cagaattttc tgcaggaaat atgtatgagt
    87181 cagcatgaac atatatgata agcagccatg accggttatc ggatccggtc atcaactagt
    87241 tctttcatgt gatatttttt actcaattca tcgagcaagc aattcatctc gcgcagaaaa
    87301 tcgggcatga tggaaatctg aatcaccgcc acggcggcat cgatggtcgt tacggtagcc
    87361 aggccctcgt acccctcgat aataaattgc actgcgacga tattcttgcg atccagctga
    87421 aaatacttgc tcatcatccg ctcatggtct cttggcttca gttatcgttt gacgtcatca
    87481 gataaagact gtatgatcaa taaaaaatca aggatttttt atgcgccttg tccgaaatac
    87541 atcgattttg ttattattat tcattgtttc ctgctccccc atccatccct tcatcaaaag
    87601 catatcggaa agcgatgttg cctctttctt gcagaacgct ccccgggcgg aggaacatcc
    87661 gaacgccggg gccgatttac tgtattccta cagttatgtg gaattctttg aagacggccg
    87721 aagcatcagc agaaacctgg atcgcattaa aatattcaac gaacgcggcc gcagcttcgc
    87781 ctcgaaatca atttcgtacc gggaaggcta tcagaaagtg aaaattattt ttgccaatac
    87841 catcaaaccg gacggaaaaa taatcgctct ggacagaaaa gatatcatcg attcttcgga
    87901 gtacgccggt tatgaattct acaccgacat caaagtgaag aagttcacca tgcccgcggt
    87961 tgaggacggc tgcattatag aaatagccta tgaaattgaa aatttaaaac ccgttttatc
    88021 catggactac tcggaaacgt ttttttgcca gaactttttc cccatcgaag aagacatcat
    88081 ggaaatcgtc ctgccggccg gcacaaaact gcaatatgaa aatttcagaa ccgacatcac
    88141 accccaaatc acttcgttgg gaaacaaaat aaaatatgtt ttcattaaca agaaacagaa
    88201 ggaaattatt cccgaatccc ggatgccctc cctgctcgat cgcgacacat ttccccagat
    88261 ttctttctgg acgctaagca actgggacat catttcccag tggtacatca aacttttcaa
    88321 agaacaaatg aagtccagcg ccgaactgga aaatttcacc cgcgagctta ttgccgatca
    88381 aaaaacggat gaggacaaaa tcaacgccat ttttaatttc gtatcccaga atatccggta
    88441 catcgccgtg ctcctgggac cgcataccca caagccccat caggcgtttg aagtgttcga
    88501 aaagcgttac ggcgactgca aagacaaaac cgttctgctt ctgaccatgc ttaaaattgc
    88561 cagcattcaa ggcttgccgg ctcttgttcc ggccaacgga aaatatttcg atgaaacgat
    88621 tccatccatg aacgccttta atcatattat tgccgtcgtc ccgctgaaag gcaaatcctt
    88681 ctggctggat gccaccaatg aaacgtcatc gtacgattcg cctccgttca tgctgcccac
    88741 cagaatcttt ctgatcaacg atgacggctc ataccgtttc gtcacgaccc cgccgttaaa
    88801 cgacaagaaa gattactatc atctggcgat tcgctataag atcgatacgg aaggcaacgc
    88861 cgctattgat tatgtttata attttttcgg caaagcggcc gaagccatcc ggtattcctt
    88921 caaatatgct cctccggagc aacgaaaaaa attctttgag aaccgcggaa ttgaaatcag
    88981 agaccttgaa ctgggaagct tttcagacac ccgaacgccg tttacaataa aactttccgg
    89041 catgattaga aatctggcac aggttctcga cgaggaaacc atggtccttt ccaatattat
    89101 cccgctggat gcataccggg acatcacagc cgccagcatc cggcggtacc ccatcagcct
    89161 gacccaatcc atgtatgcaa tggagaataa tacctatcag tttccgccgg gctttaaaat
    89221 aaaaaaattg cctcaaaatt atatatttaa aaagcctttc cgctaccgaa aagaaaaata
    89281 cagttttaca ggatccgaat ttcgtgttta tctggaaacg aaaaacgaag agcagaacat
    89341 tcctctcggc aaaatgaatg agtttaaaaa atatgcttcg gaactgcaaa agcacgaatc
    89401 agcagtaaaa aacattattt tttataaaag ataccaaaac cttgacaccc ggaaagacat
    89461 tgatattata aatcataagt aacgggaaaa gaaacggcac aatgacaaca caaagaaaat
    89521 tgccatcgct gaaatggaaa atcgaggaag ccggcaataa aaatgacggg gctctcttaa
    89581 gcaaagagtt tggcattcat ccgattattg cgcagattct cctcaacaga ggtattcgcg
    89641 atgtcgataa ggcccgccat tatcttcaac cgtctttaaa cgatctacac agtcctttcc
    89701 tgatgaaaga tatgcgccag ggtgttgccc gcctgctgaa agccattcac aacaatgaaa
    89761 agatcatgat ttacggcgat tacgacgccg acgggataac atcggtcgtc attctttata
    89821 aattcataag agaaataacg cccgtcctcg attattacat tcccgacaga attcaggaag
    89881 gctacggctt aaaagaatcg gcaatcaacg agttcaaaaa aaacggcgtc agcctgatta
    89941 ttacggtcga ttgcggaatc tccgatgtgg aacagattgc ctatgcccgg tccctgggca
    90001 ttgatacgat tgttctggac catcatgaaa tatcgggcgg actgccgccc gcagcagcgg
    90061 ccatcaaccc caatcgcgac gattgccctt accctttcaa aggtcttgcc ggcgtgggca
    90121 tcgcgtttaa ctttctcatc gctctgcgcg gcactttgaa caaggaaggc ttctggaaag
    90181 acaaccatta tccgaatctc agagaatatc tggatatcgt tacgttggga acgattggcg
    90241 acatcgctcc attgacggac gaaaaccgaa tcttcaccaa aatcggcctg gagttgatca
    90301 ccgagggccg ccgcccgggc atcaaggcgc tcaaggaagt cagcggggtg gataatcagg
    90361 tcatcgattc tttcaaggct tccttttccc tgattccccg gatcaacgcc gccggacgca
    90421 tcgcctcccc ctccgacgcc gttaaattgc ttctgaccga cgatctgtcg gaagcgcggc
    90481 ctctggccga aaagcttgat tcctacaatc gccatcgcca gttgatggag aaaaaaatat
    90541 tgagcgacat tctggaaatg atcggcaaaa accgttccct ggaaaaaatg aattccctcg
    90601 ttttcgcctc cgataaatgg catccggggg tgattggaat cgtggcgtcg cgtctggttg
    90661 aactttttaa tcgcccggct tttgtcatca gtatcatcaa cggcgtgggt aagggttcgg
    90721 ggcgcagcat ttccggtttc aacatctaca aaggcattca gcaatgcgaa cacttactgc
    90781 tttcattcgg cggccactac catgccgcgg gtatatccat cgaagaagcc aatatcgaaa
    90841 agttttccgc tgtcctcgac gaactgaccg gggacattgc cgaattttct gaaatcgctt
    90901 ctcaaataac gattgataca gaatgtggac ttgaggatat cgacccggct ttactggccc
    90961 agatggaggc tctggctccc ttcggcagca ggaatcctga accgctgctt ctcgcccaca
    91021 atgttaaagc atcatctctt gttgtcgtcg gaaacaatca tctaaaaatg agcatcaaca
    91081 gcaatggaac ctcccgcgat tcgatctggt tcaacatggg gaaacatctc tccgctctgg
    91141 acggagccag tctcgatatt gtctttacgc cgcagatcaa caactggaac gg
//
LOCUS       DAONQZ010000003        80749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_296_length_80749_cov_15.453206, whole genome shotgun sequence.
ACCESSION   DAONQZ010000003 DAONQZ010000000
VERSION     DAONQZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 80749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80749
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_80749_cov_15.453206"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1130)
                     /gene="htpX"
                     /locus_tag="PLR13_01025"
     CDS             complement(237..1130)
                     /gene="htpX"
                     /locus_tag="PLR13_01025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HQC17939.1"
                     /translation="MKRILLFLATNIAVLVVLSIVLRLLGVDRILDESGMNLNMVNLL
                     VFAAVFGFGGAFISLAMSKWMAKRMMGAQVITNPQSATETWLVDTVKKHAQMANIGMP
                     EVAIFNSPSPNAFATGMNKNKALVAVSAGLLHAMNKEEVEAVIGHEISHVANGDMITL
                     SLIQGVVNTFVIFLSRIVGHFVDRVILKNEEEGHGIGFFVTSLVAQIVFGILASVIVM
                     WFSRKREFIADAGGAKLAGTQNMINALEALKRGTAEPLPDQMAAFGINGKPSGHGLKL
                     LFMSHPPLEDRIEALKKLTYR"
     gene            1456..3291
                     /locus_tag="PLR13_01030"
     CDS             1456..3291
                     /locus_tag="PLR13_01030"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQC17940.1"
                     /translation="MKKKDIVQYPQTISGFEFFVKFMAFVGRIPFVVYHGIKGIKSIS
                     SNRNASWGSLLQKSALKYPNNPAVKSPDGTLTYAELNEKANQFAHFLLSKNVKKGDTV
                     AVCLENRPELLFLYCGCAKVGAVCSLINTNQRGDSLVHSFNLNKGKVMVVGEECYSFF
                     KDVKDRIDLQGSELCLLRDPAMNQLKAPEDITDQLAVLPKTNLSETGGISLKDTMAYV
                     YTSGTTGGLPKAAVITHKRAMSATFWFGRVVTHVKPKHTIYIPLPFFHTNAITVGWPP
                     ALYSGAAVAMRRKFSASGFLDDVKKFQATHFVYVGEVCRYLMAQPEKAGENKTTLKYI
                     IGNGLRPDIWMAFKRRFGISKIYEFYGAAEGVGVFTNLMNFDYTVGTSLTPFAIVQYD
                     IENDCPARDAGGFLIKVAKGDPGLLIMEISEKTPFPGYTDKKKTEEKIIRNAFRKGDL
                     WFNTGDMLRNMGFRHAQFVDRLGDTFRWKGENVATMEVEKALDSFSGVSASAVYGVVM
                     PKGDGRAGMAAVIKQEGKDVDFAALAEHLRSVLPKYAVPIFIRFVNDFQWTATHKIKK
                     NDLKNDGYNLSAIKDGLYVLLPGTETYVPLNDAIYQDIMAGKYKF"
     gene            3538..6327
                     /gene="ileS"
                     /locus_tag="PLR13_01035"
     CDS             3538..6327
                     /gene="ileS"
                     /locus_tag="PLR13_01035"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQC17941.1"
                     /translation="MDYKKTLNLPQTDFPMKANLAQREPEMLKKWEDMNIYKKIRQMA
                     KGKTPYILHDGPPYANGNIHLGTALNKIIKDIVIKSKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGAKKGAMSQVEKRRACRTYAEKYVDIQREQFKRFGVFGEWENPYLTMAYPY
                     EAVTVEEFGKLYLNGSVYKGKKPVYWCATCRTALAEAEVEYADHHTPSIYVKFNFVSD
                     IAQVLPKLAAQKVAMVIWTTTPWTIPANLAIAVKDDFIYVAVKINDDVMIVAKDMLDY
                     CLDAFGYQGKSYEIIDEFKGAVLEGQKCVHPLIKRNSLLILAPFVTLDAGTGAVHIAP
                     GHGQEDYEIGLEYGLDNYAPVDDAGKFTEDVEHFAGQFVFDANDSVIKKLDEVGALLG
                     HVPMQHSYPHCWRCKKPIIFRSTEQWFISMEKNDLRKKALECINEVTWIPSWGKDRIY
                     GMVENRPDWCISRQRLWGVPITVFYCAKCKNEVMTREMMDHLVALVEKNGADVWFEKD
                     PKDLMPKHTTCPHCKGTEFTKEVNILDVWFDSGVSHAAVLEKRPDLSSPCDMYLEGND
                     QHRGWFHSSLLESVGTRGRAPYKNVLTHGFVVDGEGKKMSKSVGNVIGAEEAINKYGA
                     EILRLWVAAEDYTDDIRISEEILKRLMEAYRRIRNTSRFILGNLYDFNMDKDAVAYEQ
                     MSELDRWALHRLQEVIKRVTEAYERYQFHVVFYTLYNYCTVDLSALYLDVLKDRLYTN
                     KAASAVRRSGQTAMFIILNAMTRLLAPILTFTSEEVWAAMPDWTNKEDSVHLAQFPEV
                     SGTFFNADLGERWKAMIDAKSEIAKAVEQARKEKIVGHSLDARITIAAPEKLRALFAA
                     HLEDLKALLIVSQLQLADEQDIAAPFRSAEIEGLMVGVEKARGAKCERCWIYDESVGA
                     DAQHPTICARCLPNL"
     gene            6343..6831
                     /gene="lspA"
                     /locus_tag="PLR13_01040"
     CDS             6343..6831
                     /gene="lspA"
                     /locus_tag="PLR13_01040"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQC17942.1"
                     /translation="MKKNIIIFVLGAAVIVVLDQITKAAIVKKFFLHETRSVIDGFFN
                     LVYVMNPGAAFGFLAGMSEMFRSLFFIGMTLLVMALIIYYIAKSKSENMFFVVSLTMI
                     FAGAVGNLIDRIRFGAVVDFLDFYIKTAHWPAFNVADSAITIGAVLMIWGMVAGRKRE
                     AV"
     gene            complement(6910..7203)
                     /locus_tag="PLR13_01045"
     CDS             complement(6910..7203)
                     /locus_tag="PLR13_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17943.1"
                     /translation="MSLLIGGIISAIFGLVSLIFFWPDFMTIFKGSLPIFLLLGGALA
                     VYVGIDEIQEKNRQERHLQEEKLEHAQKEIETIRAQAESYREELQKLKDSKQP"
     gene            7367..7741
                     /gene="hisI"
                     /locus_tag="PLR13_01050"
     CDS             7367..7741
                     /gene="hisI"
                     /locus_tag="PLR13_01050"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HQC17944.1"
                     /translation="MIEPDFAKGDGLIPAIVQDAETKDVLMMAYMNRESWDATLKTGK
                     ATYWSRSRKKLWLKGESSGNVQIVKAVFIDCDEDTILLHVQQIGDAACHTGHRSCFYR
                     KLEGNDFVAVGEKIFNPEDVYK"
     gene            7738..8610
                     /gene="hisG"
                     /locus_tag="PLR13_01055"
     CDS             7738..8610
                     /gene="hisG"
                     /locus_tag="PLR13_01055"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQC17945.1"
                     /translation="MKNLKLGIPKGSLEANTIDLFKKAGWRITSDARSYFPDIDDPEI
                     SCKLVRAQEMSRYVEDGTLDLGLTGIDWIMENDSDVVVVQDLIYSKVSTQQARWVLVV
                     RDDSPYKKIEDMEGKRISTELVNFTKKYFAERNINIHVEFSWGATEAKVVEGLVDAVV
                     EVTETGSTIKANKLRIIHELMKTNTQLIANRAAWKNAWKRKKIEQISTMLTGSLQAMG
                     KVGLKLNVSKENLKGVIGLIPSLKSPTISGLSSDKWFAIESIVDAKVMRDLIPKLLDA
                     GAQGIIEYPLNKVI"
     gene            8625..9212
                     /gene="hisB"
                     /locus_tag="PLR13_01060"
     CDS             8625..9212
                     /gene="hisB"
                     /locus_tag="PLR13_01060"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQC17946.1"
                     /translation="MARKAKIVRKTTETDIRLEIDLDKTAGSSIETTIPFFNHMLELF
                     ARHGFLKLIVRSKGDTHIDDHHLVEDLGICLGQAVAKALGDKKNINRYGSARVPMDEC
                     LCQVDMDICGRPYLIYHVKSGRRKIGKFDPALVKEFFKAFTDQSGITLHINFLYGENI
                     HHMIESIFKAFGRALRNAVSLNENIKGVLSTKGKL"
     gene            9319..10005
                     /locus_tag="PLR13_01065"
     CDS             9319..10005
                     /locus_tag="PLR13_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17947.1"
                     /translation="MVAILRKYFCFIVRFVFVALLAFPVMSQALDKKAQSDESENLIA
                     VIAFQPLRPEGGTGNTVLCPICGKASAGGIIQEGAEKIVEEIFVEQLKARDDIVLVPA
                     EKLQKATQKYSTDSMKDFQATGLLKIGRELKADFLVVGYVYRFAERVGYRYSSEHPAS
                     VVFEIHLIRTADGKSVWRGYFDKTQRSLMENVLDISSFFKGGAKWMTARELTELGMEN
                     VFQTFPDFQP"
     gene            10158..10895
                     /gene="hisA"
                     /locus_tag="PLR13_01070"
     CDS             10158..10895
                     /gene="hisA"
                     /locus_tag="PLR13_01070"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQC17948.1"
                     /translation="MIIIPAIDIKDGKCVRLAQGKFDRVTTYADNPLDMALLWARKGA
                     ELIHIVDLDGSVAGRPRNADIILNIVENINVPVQIGGGIRDMETIRFYLDQGVSSVIL
                     GTTALKNQQIVREACTVFPGRIILGIDALNGKVAVRGWTENTEKNAVDLARQYEPYGV
                     KAIVYTDIERDGMGTGINMKATRELADSVSIPVIASGGVASIADVDNLMVVEGGSIFG
                     VIIGRALYTGAVDLEEAISKTKKVTRT"
     gene            10914..11258
                     /locus_tag="PLR13_01075"
     CDS             10914..11258
                     /locus_tag="PLR13_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQC17949.1"
                     /translation="MSDCVFCKIVRGEIPCARVFEDEHVLAFDDIHPMAPVHVVIIPK
                     KHIATLMDVDVDNSDVIHRLTAAAQKVARIKKIDQSGFRTAINCNAEGGQVVFHLHVH
                     VLGGRKLQDELG"
     gene            11262..11723
                     /locus_tag="PLR13_01080"
     CDS             11262..11723
                     /locus_tag="PLR13_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQC17950.1"
                     /translation="MEGEKMDINAKLSEEMVAAAKAKDKIRLSAARMLKTALHNKEID
                     LMRPLHESEMLQVLSSMIKQRKDSIEQFAKGGRTDLVEKEEAELKFLQEFMPAQMSDE
                     EVDALIKKTIEDAGAVSVKDMGKVMKILMPKLTGVADGKAVGEKVKAFLSQ"
     gene            11771..13543
                     /gene="dnaG"
                     /locus_tag="PLR13_01085"
     CDS             11771..13543
                     /gene="dnaG"
                     /locus_tag="PLR13_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQC17951.1"
                     /translation="MYVDQSKIEEIKNRLDIVDLVSEYLTLKKAGRNFIGLCPFHQEK
                     TPSFTVNRDKQIFYCFGCGEGGNVITFLMKIADKTFPEAIRDLAVKTGVVLPPVSSGK
                     EARQKDLLKENIVSLNSKAAQQFSRNLFSASGTPARQYLQQRGIAEDVVKQFRLGYAP
                     DTWRSLTDFMEKSGLSLKLAEQAGLIIPGKEGGFYDRFRGRLIFPIENVFGEIVAFGG
                     RILGKGEPKYLNSPESPVYIKGKNLYGLNKAKDDIRKKDFALIVEGYFDLISLWNSGI
                     RNVVATLGTALTRDHLDLLRRYCVDVVALFDPDEAGKKALDRSLELFLSTEMRARALI
                     LPDGYDPDDYVRKFGQDKMEDLIALAQPLSDYYIENVIGGGRTFEDKRDMVKTAMTFL
                     SKIGDQKEKNLFIKRIAEKTGLNEELLIREAYSRVYSGRTETNRNNPAVNVHHDTVEL
                     HLIQLMMEYPQKIVQVENENVLDYFLQPDIRKLGKKIIDAYKLLGFVDVGVILSTEEN
                     QALRERIFQLMIKALPAEDDVLGKILTDNIRQIKKRWFKEQHRQIKIKLSRAQDSGNE
                     EMVHQLLYEKEQMLIKEKELQSTG"
     gene            13649..15229
                     /gene="rpoD"
                     /locus_tag="PLR13_01090"
                     /pseudo
     CDS             13649..15229
                     /gene="rpoD"
                     /locus_tag="PLR13_01090"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            15454..15529
                     /locus_tag="PLR13_01095"
     tRNA            15454..15529
                     /locus_tag="PLR13_01095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15487..15489,aa:Ile,seq:cat)
     assembly_gap    15793..15892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16240..16959
                     /locus_tag="PLR13_01100"
     CDS             16240..16959
                     /locus_tag="PLR13_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQC17952.1"
                     /translation="MKEQLLLLIKIQECDSELVKLSAKKKTLPEKIETLNKEFNAFED
                     GIKTSKVKYDELKLRHTDSENKIKKINESIVKTKERMLEVKNNKEYQAMLKEIEIAEK
                     SRGEIETSIISILDELDKLSVLVKKDDDVLKERRHQYEQEKKTIEDELNAVDTDAANW
                     EQKRGDLQKNVPEDLLVRYERIKKRNRGVGVISVWKSVCNGCHMNIPPQLYNEVQKSE
                     ELFSCPNCNRIIYFKDMVKPS"
     gene            16965..17375
                     /locus_tag="PLR13_01105"
     CDS             16965..17375
                     /locus_tag="PLR13_01105"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HQC17953.1"
                     /translation="MSEKYYKLFSDGACRGNPGIGGAGAVITDDADHVIWEGKEYLGH
                     CTNNIAEYKALILGLKGALSQGYRNLQIFLDSELLAKQINGAYRVKNENLQTLMKEVR
                     HLLVSFDRVEVKHVARLYNSHADKLANLAIDEKN"
     gene            complement(17402..17881)
                     /locus_tag="PLR13_01110"
     CDS             complement(17402..17881)
                     /locus_tag="PLR13_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17954.1"
                     /translation="MLKDYQMQLLGFLEQLEMEISNLYKSFAEQFPGHQDLWAEMSRE
                     ELQHAEYVRTLKAFALSGHVTFDEKMTKTYTIRSIIDDIRDKYKKTENHQYSVVNALS
                     FSLSLEQSIIEHKFYNYFSSNDPGAVLLINNIREETSAHESQVKKALDEEKKRITVR"
     gene            complement(17901..19046)
                     /gene="proB"
                     /locus_tag="PLR13_01115"
     CDS             complement(17901..19046)
                     /gene="proB"
                     /locus_tag="PLR13_01115"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQC17955.1"
                     /translation="MENIRQNIITRAKKILIKVGSAVLTGDDGLDLKIIDSLVLEMAN
                     LAKKGYAVVLVTSGAIVSGKHRLKITENLKSIPEKQAAAAIGQGRLMRVYSKAFEKNG
                     IYVAQILLTLSDLTDRQRYLNIRNTLSTLMAWGTIPIINENDTVAVDEIKFGDNDNLA
                     AMIANIIEADLFINLTSIDGLYTCNPASSKKSKLISVVSEFSEEIEAAATEETSSSGT
                     GGMKSKVQAAKKVTAIGIPCIIAPGKKKNILTDIMTGREIGTLFLPMTDRLNSKKYWI
                     AFTLRPRGKLVIDDGAKKALLEEGKSLLPSGIIDIEGRFDHGDPVICVDNNGAILAKG
                     LVNYNSIEINKIKGLKTKQIGQVLGHKDYDEIIHRDNMAVTGQHYKK"
     gene            complement(19066..20061)
                     /gene="obgE"
                     /locus_tag="PLR13_01120"
     CDS             complement(19066..20061)
                     /gene="obgE"
                     /locus_tag="PLR13_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQC17956.1"
                     /translation="MKFVDEAKIYVKAGHGGSGCVSFRREKFVPKGGPDGGDGGKGGD
                     VIFRTTESHHTLLDLKYKQHQIAKNGGHGSGNHRTGKSAPDLEISVPVGTIIKNFDTG
                     EELADLSKAGQTYIAARGGIGGKGNSHFATSTHQTPRFAQEGMEGDEFTLKLELKLLA
                     DVGIIGFPNAGKSTFISRVSAAKPKIADYPFTTLTPHLGVVQYADDKNFVIADIPGLI
                     PGAHEGMGMGDKFLKHVERTSLLLHIIDVSMTPHTDAWSNFSTINDELKKYNPALAEK
                     EQIAAINKIDLPFVREKIKKEVALFKKKGIILHAFSAVTGEGVKEILNRIAEKLK"
     gene            complement(20257..20511)
                     /gene="rpmA"
                     /locus_tag="PLR13_01125"
     CDS             complement(20257..20511)
                     /gene="rpmA"
                     /locus_tag="PLR13_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQC17957.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGIKVFGGEQIHAGSIIVRQVGTK
                     IHPGNNVGMGKDFTLFSLINGVVKYERLDKTRKKVSVYAA"
     gene            complement(20524..20835)
                     /gene="rplU"
                     /locus_tag="PLR13_01130"
     CDS             complement(20524..20835)
                     /gene="rplU"
                     /locus_tag="PLR13_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQC17958.1"
                     /translation="MYAVIKTGAKQHKVSEGDILSVEKLDGNKGDEVVFSDVLMVSDD
                     KEIKVGKPFVDGAKVVGEIIAQKKGPKLTVYHMKRRKGFHKKTGHRQNLTSMKIKKIS
                     I"
     gene            21174..22001
                     /locus_tag="PLR13_01135"
     CDS             21174..22001
                     /locus_tag="PLR13_01135"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC17959.1"
                     /translation="MNAGAPVALISGCSTGIGRFLTTELAKRGWQVFATARDHAAIED
                     LKAPNVQTAALDVTDEKSIKTCVESVMANAGKIDMLINNAGLLLIGPLSELEISELRR
                     EFETNVIGLAALTHAVVPHMIKRTSGKIVNISSISGVLNTPFSGAYCSTKAAVTSYSD
                     VLRMELKPFGIDVITVQPGGIRSNLSDNADKGLERFRKTPYAPITDFIVDRAHMSQQG
                     ATPTDVFARQLVDKLVKSKTPRIIRIGKGATMYPFVAKLPRTFTDRLLSRKFGLDKL"
     gene            22139..23008
                     /locus_tag="PLR13_01140"
     CDS             22139..23008
                     /locus_tag="PLR13_01140"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQC17960.1"
                     /translation="MKYGIIANPVSGKRSVDDKIKILKEVAGILKDATIAGLDTKSPE
                     EFSTAAHDLSQKVARLLVAGGDGTFSLVMNSVSRDIPLGFLPFGSGDVLRHTFNLPRC
                     PKENALLIQKGKEYLLDVICCDGKTKALFASIGIEGSILLERDNYLENNLTGFTAYGL
                     STLKILFDGYIKADLRVLMDKKEIRTFNSLTAVITKLPYYGYGFKVVPHAQFDDGYLH
                     LLILNAGPIGTLYALATSLAGGNRMGEYYKAKEIDITTSTEQLLQRNGDPEKEKRNNF
                     SFRILPKELKLIF"
     gene            23122..23808
                     /locus_tag="PLR13_01145"
     CDS             23122..23808
                     /locus_tag="PLR13_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQC17961.1"
                     /translation="MRQFNSDRRLIVKTAQKLVEKGYLAATGGNISVRIKKGNAFAIT
                     PSNRNYMTMTAADICILDEHLNVLSGPWKPSMEAALHGAVYRTRGDVNAVIHTHQVYA
                     GALTIIKTPLPALFDEQVRFLGKTVAVIPYAPSGSVPLKNNVLRHIKNGNNAFLIQNH
                     GALVLGHDMDRAVHNVEILERYSLAYLLAICSGYRVSKIPMRMRETFFKMLRQEQKKL
                     QKDHCGSRKK"
     gene            complement(23827..24528)
                     /locus_tag="PLR13_01150"
     CDS             complement(23827..24528)
                     /locus_tag="PLR13_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HQC17962.1"
                     /translation="MRIWDVNPGYLNRQSLLGEHRELHAIVSIIQHNKKGYSRHPETL
                     RWHGFGWALSQRHRLLAAEMNLRGFTDRSPAMIKTQPQKWPHVFVDSPTSQFSILKKK
                     YVNKEQGRIPLPNSVQQLWAQHQYSVMARDEAEYKYIGGWVTSRKSGKGIHEIYPELV
                     SLLRCPPVEANLRNALEQMWNFVCAYAPHPATAMKKESSRHLLKQIQRTAVLHDIVYL
                     KESTALGELPAWQIG"
     gene            complement(24550..27051)
                     /locus_tag="PLR13_01155"
     CDS             complement(24550..27051)
                     /locus_tag="PLR13_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQC17963.1"
                     /translation="MPKKEKIIMRHGKIDELLSSAEKPSRYIGTEVNAVRKDKADVRF
                     LLAFPDTYEVGMSHLGLQILYSIINDIPYASAERCFAPWPDREKILRDKKLPLTSLER
                     QIPLQNFDMIGFSLQYELSYTNMLNMLDLGGIPIDRKERRDGHPLIIAGGPCCFNPAP
                     LADFIDAFVIGEGEEIVGDIVAAVREGKIKSISRNGLLGELANIPGMYVPAVHHDNEI
                     IKKRTVSDLNAWRHPLKPVVPLMQTIHDRITLEIARGCTRGCRFCQAGMIWRPYRERN
                     AALLMEMAEKMLAATGHEEISLLSLSSGDYSCLEPLVKGLMNRYCQKRVALALPSLRV
                     ESLNTTLMEEIRRTRKTSFTLAPEAGTDKMRLVINKGNTSEDLLSTADKIFAAGWKSI
                     KLYFMLGLPHETQEDWEGIVNLSHETLRAAKNRGQVTISLSTFVPKPHTPFQWEKQIS
                     LAETYERQDFIRTRIKNRNLSVKWHDARMSFLEGIFSRGNEQAGKLLGNAFRKGCRFD
                     GWSDILRFNLWEEAIAESDIHPDDYLRERGTDEKLPWGNIDCGVSREFLLEEKQKAFQ
                     QTATADCRFETCRNCGACDFSITKNILAADNENKSVLPSSLAPETSSAQEEKYRVMFN
                     KMESAGFLSHLELATALVRALRRSSINLAYTGGFHPHPKISFATATAVGMESRQEYMD
                     INAQEYSCSLDLLRTEINTLLPTGIKILEIRKLSPEENAIAQATRGFEYELQLPAATD
                     APRMSAIKANIDQFLAASNFNIKKISKGKTITKDIRPFVQMLSLDRDKKIIKFIVSFV
                     QEGSARPVDIIAHILKSDPDESRQIRVVKTKTLLG"
     gene            complement(27063..27461)
                     /locus_tag="PLR13_01160"
     CDS             complement(27063..27461)
                     /locus_tag="PLR13_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17964.1"
                     /translation="MKLIEEIKLDNGLDLRIFDLSRSIATDTVKVEVSFQTNVLLKES
                     FFTSTEDYRLVKNIMGDELAYEHTMERTFVSKDNEDSTRNELISTFKHNSLGYLSAAN
                     FAQKMALSKLREIKSNPHKYRSHAQSDKKA"
     gene            27574..28431
                     /gene="purU"
                     /locus_tag="PLR13_01165"
     CDS             27574..28431
                     /gene="purU"
                     /locus_tag="PLR13_01165"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HQC17965.1"
                     /translation="MNNVNAVLLLSCADSKGLVARISDFIFRFDGNIVHADQYTAKAE
                     KLFFMRVEWELNGFALDRDDIPVAFAPLAKQYQMKWDLHFTDYVPRAAIFVSRHLHCL
                     HDLILRRDMGELAADIKAVISNHEDARDLAEQYGMQYFYFPVTGKNKKQQEKKELRLL
                     RDLHIDLVVLARYMQILSGNFLKYYPSRIINIHHSFLPAFAGRNPYQQAHDRGVKIIG
                     ATGHYVTAQLDEGPIIAQDVVKISHRDTVTDIQRKSKDLERIVLARALRLHLENRILV
                     YGSKTIVFE"
     gene            28602..29420
                     /locus_tag="PLR13_01170"
     CDS             28602..29420
                     /locus_tag="PLR13_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17966.1"
                     /translation="MSNSNIFVLCQPDKGKSCGACCGLYNYVDSSRSSLARRLRSRTK
                     RFRETVKSPADLKRYATETLVAEDFKKRYDVIHCCEYLGFLDHDEKKVGCLVHPEQNA
                     GCDMRDVSFYGRELCAGHLCPSHYFIPQSQSQILIKIIDDWYLYGLVLTDIDLVVQYF
                     RLIADRVGAELKPDVFDHDELKEIALDYFGWKLNWPFRSQEANRLGKYYFDGSQYMIS
                     HIEYEKFGREVSVLNSILLSLCSSFKSAVQLEEAEKMIWANIEKFVSTYKRHVS"
     gene            29610..30143
                     /gene="hpt"
                     /locus_tag="PLR13_01175"
     CDS             29610..30143
                     /gene="hpt"
                     /locus_tag="PLR13_01175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQC17967.1"
                     /translation="MDQASRKILFPKETIQKRVREMANQISKDYAGKELILIGVLKGA
                     FIFMADLIREINIPCRVDFARLASYGAGSESSGKVVLTKDIETSIKDKDILIVEDILD
                     TGLTMQYFVEWLKERNPKSLKTCVFLDKRKRRKVPLEADYVGFTIDDGFVVGYGLDFN
                     EMYRFSPDIYTIHSNKL"
     gene            30225..31259
                     /locus_tag="PLR13_01180"
     CDS             30225..31259
                     /locus_tag="PLR13_01180"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HQC17968.1"
                     /translation="MIIQCRQCRTKFRFDDSHMQGSGIWLRCSRCGHVYFQENPFAKR
                     TDSGALPLTEPDLETKPVNEEEKTAPSETVSVPPEEAPSIIRRDEHVLKSIDKIMTAR
                     KRDREGIDLDIATFRDEKVGEADGQTGDIVQEEQEVNVEETDHQKKTLPKGSGRIWKT
                     ALWSVLVIGVIPLLIYFFILPQYGAQLVGSGEYLFKKISELRGAPELPTSGAYINQMV
                     KLQNVRYRRINNYILGQIGVLEGTALNRADFPLSRVQVKGEIVDAYSVVLGERSSYAG
                     NILTDEELINLSEEEILMKLLRPEGLNNANDRIMPNGQIPFMIVFPSEPSGVIKTTVM
                     TIGAERLLEP"
     gene            31330..32208
                     /locus_tag="PLR13_01185"
     CDS             31330..32208
                     /locus_tag="PLR13_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC17969.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTVGCSHNQCTFCGMYKDKKYRER
                     SLDEIMEDIAMAKDHYGDLEKVFLCDGDAIALKTDMLLTILKKLYGTFPSLRHVGSYV
                     GPQSTLSKSLEELQTLRAAGLTKAYIGVETGDDELLKKIKKGVGYEEMLQAGRNLIAA
                     GINLSAMVLLGLAGRGKASRRHALATAKICNEMKPQYLAALTVTPVPGTVLYRQVQRG
                     EFELLDAFETLEEMKQIFENITADNMKFVGTHASNYLPVVGTLQKDKQKMIAMVDEVL
                     KNRDERMLRPDHMRGL"
     gene            32385..33722
                     /locus_tag="PLR13_01190"
     CDS             32385..33722
                     /locus_tag="PLR13_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17970.1"
                     /translation="MMANVMDKYFNMRIILCVALMSMVLLLQACSINQVYLSDSDEED
                     YSAEDITDQEMMRVAKVKLQSTGMVKRAFDIEERHRKLTLRETRQLLKILSQRFKKSG
                     LSVGEFEDLVLERIGRDLEIISAFNERLKNAEAKEKDHGYELCKKNELLEVIKGSEDI
                     VEEYIEDMVNGILPEEFETSLSEITRLERTKEDDIVPGSFKPTCFKGALITMLVALRG
                     DRANVSALKLSGGTWSNSSIIFHTIPMPGKISRSSIHPDFESFTYYYKGASSPSQGEL
                     GFPHSGYYAIGYGARKSSKGIANPEDCSSWIFHQVLQTQYPVTSYDVMQFFRIKTGTG
                     IRHTGYAATPEYGWLNSVFIPVEKITKKNARAMIKPGYVLCIRRPDEDGKVIRNQNYG
                     VSGHMAIALFATDTHIFAVACTRDVPLMEGIGIQKISFWKERNSDIMILRMNK"
     gene            complement(33869..34507)
                     /locus_tag="PLR13_01195"
     CDS             complement(33869..34507)
                     /locus_tag="PLR13_01195"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQC17971.1"
                     /translation="MPEILKPGTDISEDAMISRAVKILTDGGIIAYPTETFYGLGVDA
                     THEEAIGKIFAVKGRNFKNPVSLIIAKPEDINVLVTDVPETAKKLIDAFWPGALTIVF
                     QASDTLSPLLTAGTGKIGLRVSSDPIALKIVQNIKKPLTATSANLSGAPECSTASEVA
                     GQIGGKIDAILDGGKTPGGASSTLIDVTLNPPVVLREGSISRQSIKEHLHVI"
     gene            complement(34492..35001)
                     /gene="purE"
                     /locus_tag="PLR13_01200"
     CDS             complement(34492..35001)
                     /gene="purE"
                     /locus_tag="PLR13_01200"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQC17972.1"
                     /translation="MVKRNALVSVVMGSDSDLPVMTEAAKVLEEFGIAHELILTSAHR
                     TPLRTTQFASAAAGRGIKVIIVGAGAAAHLAGVIASQTPLPVIGVPIDATALNGLDAL
                     LSTVQMPGGIPVATMAIGKAGAKNAALFAVRMLALDNKTLGKKLRDYIRKMAKDVEKK
                     QENLTCRKS"
     gene            35197..35925
                     /locus_tag="PLR13_01205"
     CDS             35197..35925
                     /locus_tag="PLR13_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HQC17973.1"
                     /translation="MKNNLYEFLAGNDYPGRGICIGMTPGGKAVIAYFIMGRSTNSRN
                     RVFDPIEGGIRTKAADPSKMTDPHLIIYNPVLIFAKTTIVTNGDQTNTIYDFMSQNKF
                     PGFHFEAALNTRTFEDDSPNWTPRISGMVDMKTGAYKINILKSCGGDEKSVQRFTFNY
                     AQPLSGEGHFISTYKCNGTPIPSFQGEPMRVIIDEEDPDRYAAKLWDALNEENKVSLF
                     VRAVDLKTQTCKDVIINKYKAVEE"
     gene            35928..37106
                     /locus_tag="PLR13_01210"
     CDS             35928..37106
                     /locus_tag="PLR13_01210"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF005492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HQC17974.1"
                     /translation="MNEIQLKYGCNPNQKPARIFMADGSHLPVDVLNGKPGYINFLDA
                     FNSWQLVKELKENTGMVAAASFKHVSPAGAALGYPLDRVLRKMYHIDSKIKLSPLACA
                     YARARGADRMSSFGDWIALSDACDVSTAKIIKNEVTDGIIAPGYEPEALEILKSKKSG
                     NYNVVKINPDYVPVSLEHKQVYGITFEQGRNDLKIDSRMLENIVTENKTLTDAQKRDL
                     VLALITLKYTQSNSVCYVQDGQVIGVGAGQQSRIHCTRLAGQKADNWQLRHMPKVLNL
                     PFRDDVAKPNRDNAIDVYLGETPEDVIGDDVWGQTFTKQPKSLTRVQKQKWLSKVTGV
                     CLGSDAFFPFGDNIERARRSGVTAIVEPGGSIRDQQVIDTCNKYRIVMAFCGLRLFHH
                     "
     gene            complement(37248..>37532)
                     /locus_tag="PLR13_01215"
     CDS             complement(37248..>37532)
                     /locus_tag="PLR13_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17975.1"
                     /translation="NDFDLSQSGWGLDATWSHRLNSPKDKIAIIDDITAVHTKPVGSD
                     YSRFKIKPETELKHLLEKYNIAFIIKSYSSILKNGGHKIPWYRKFFRFSS"
     assembly_gap    37533..37632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37633..38065)
                     /locus_tag="PLR13_01220"
     CDS             complement(<37633..38065)
                     /locus_tag="PLR13_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF707 domain-containing protein"
                     /protein_id="HQC17976.1"
                     /translation="MILIYYGNSDLIFFDYTKDALICIKQHGQKFPLIKSFIETNLEL
                     VSQYDYVWLPDDDISISAGDLDRLFDTARTYSLQICQSALVSLHGDVSHAITKPEREN
                     TLRFTNFVEVMMPLFDVNTLLFLRNDFDLSQSGWGLDATWSH"
     gene            complement(38464..39543)
                     /gene="mutY"
                     /locus_tag="PLR13_01225"
     CDS             complement(38464..39543)
                     /gene="mutY"
                     /locus_tag="PLR13_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQC17977.1"
                     /translation="MMKDTDKIKKLLNRRLLAWYNRNQRLLPWRKTNDPYRIWISEIM
                     LQQTQVDTVIPYYYRFLETFPSVSSLARAPLQDVLKVWENLGYYSRARNLHAAAKVIV
                     EKFGGQIPDKLEEIKTLPGIGQYSSGAILSIAYKQAFPAVDGNVRRILCRVFAIRKPV
                     DDAREQKQLQELAASLIPVNHPGDFNQALMDLGATICKAKNPDCSRCPVANLCQARIK
                     SLQNVLPIIKKTPAIPHRQAAAAVIRNAKGRLLLVQRPASGLLASLWKLPGGFVETGE
                     DIERSLRRSVKEELGISIQTEKHLASVNHTYTHFRITLHAYNCRLLKSSPKPIGCQNS
                     RFVSPVDLKKLPMSKIDRLILDEIL"
     gene            complement(39825..40460)
                     /locus_tag="PLR13_01230"
     CDS             complement(39825..40460)
                     /locus_tag="PLR13_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_272502274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(40233..40235),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HQC17978.1"
                     /translation="MNHFKKYFLTFLLVTCLPIIGWASDQQEITAAIDYPQLEKRIAD
                     LSVTNMKGEEVAFESLWKHNRVVLVFIRHFGUMFARQRAAALMEIKKQIEANGAKILI
                     IGSGTPEQAKTFAENFNVELDIYVDESLQTYNAFSLERGFFKTLGWKSLKCGVRAMQS
                     GFHQGLSAGDLWQQGGVFVLGPGKQIVYQHVEQYAGEPLEKDEVLQAIKKR"
     gene            complement(40518..41387)
                     /locus_tag="PLR13_01235"
     CDS             complement(40518..41387)
                     /locus_tag="PLR13_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQC17979.1"
                     /translation="MSQNTGNDNPDAILIQHALDGNRKSLEQLVADHQPWIYNIAFKM
                     IMDHDDAADITQEILIKIITNLASYNPDRGNFRTWAYRIVVNHVLNMKKRKFETRIYD
                     FDRYIAAIENFPDDSSFTHPDAGLLAEEVKTGCMMGMLLCLKRTERIAFLLGAVFAVS
                     DAVGSELMDISRENFRQLLSRSRRKVFNYMNGTCGHMNPANKCRCSHKVKSFLDLGML
                     DSAILRYHRPNSRMVKDMLGSRLERFHLSYYEPFLALFREQPFYDPPDVTSWLDDILR
                     QEDLKAIFNLGEE"
     gene            41884..42417
                     /locus_tag="PLR13_01240"
     CDS             41884..42417
                     /locus_tag="PLR13_01240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQC17980.1"
                     /translation="MASDLKAVIETSKGTINLKLFADQTPVTVGNFVNLARRGYYDGL
                     KFHRVIPDFMIQGGCPFGDGRGGPGYRFQDEFVKELRHNKSGILSMANAGPQTNGSQF
                     FITHVDTPWLDDKHTVFGEVVSDADQAVVNKIAQGDKIISVKIEGDVAPLLEKIKPAL
                     DKWNTALDENFPKLAKV"
     gene            42438..43490
                     /locus_tag="PLR13_01245"
     CDS             42438..43490
                     /locus_tag="PLR13_01245"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HQC17981.1"
                     /translation="MRKVTETPSKMVEKDGTIHFGTFRTPFRDVNILDVPLYSSSSGV
                     PAFWKKFRLKEWQHFGIITSNYYFGMVIFDAKFMGVSFFYAYDRNENKRFEHSVQLPG
                     GAANVAAQVYDGHCEFKKKGYRLYIENRLQQGYHKIIMDIDGKKDLPAVQGEIKVLED
                     LNGIEPLVQVSPVTPFRPFYTHKVVAPAEGEIMLGRDSIKVNREWDVALMDEQKTYYP
                     YVSFWKWATAAGYNEYGELLAFNLCQNMITDDGNFNENCMWVDGKIYCLNAARFKFDE
                     QNVLHPWEVKTNDGCMDLIFTPSGERAEKISIGLIVSDFHQPFGLYNGRFKDNNNRIY
                     PITDFFGLAEHHVTRY"
     gene            43515..44156
                     /locus_tag="PLR13_01250"
     CDS             43515..44156
                     /locus_tag="PLR13_01250"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQC17982.1"
                     /translation="MFDIGNQDQKATQYPLRWDRFWARCIDFFLQAVLCFLIWIFSGI
                     GSWVSGTIHKRDLQDISHLTNPHFWQVYLIFFAMMSLAFFLYEVFSVSVLGATPGKIL
                     FKIKIADREGRKLNFYNAFKRSIYLFWFGYYFYIFTFHGFIIGYWLSSRYYKKRGTFR
                     WDQASDSIINQETLTRTRKRVYRFLAMCCLFVCMVPVPEISFSIGSLIGKLAL"
     gene            complement(44192..45328)
                     /locus_tag="PLR13_01255"
     CDS             complement(44192..45328)
                     /locus_tag="PLR13_01255"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC17983.1"
                     /translation="MPNNSTTTPYVRLGFLDSLRCFAIIYVVISHLILIPQPNLAIPE
                     WLAPFLINGGDAGVSLFFVLSAFSLSYAMDKRAKEKRLLRNFYVRRFFRIAPLFYLLM
                     LLYWIRDAAVFGVFHPFSEILVNASLLFNLVPSCITGFVWASWAIGVVTVLYLIFPVI
                     HRYIRNLHGALALFIFSILTSWSWSYFVKYYGVATGYLKADDVGFVWGFGFLQHLPVF
                     ACGIIVYHLFFDYFSKTNQKSRQTYGMLFILAFIFFYGVLLTENIQNIMWGKKILHGI
                     CFSLLALGLGLKPFNFLVNDKTASLGRTSYSMYLFHPLLIFTVIPVYRRIYNFIPTDM
                     LGYLSSLLITLFLLTIISLLSYRYIERTGMAMGEKFIVKRHPDN"
     gene            complement(45379..46710)
                     /gene="purD"
                     /locus_tag="PLR13_01260"
     CDS             complement(45379..46710)
                     /gene="purD"
                     /locus_tag="PLR13_01260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQC17984.1"
                     /translation="MPNILLLGNGAREHALAEAITRSGQKPSLFSFMKSNNPGIASLS
                     EKTLIGSYADLDAITKFATENKIEFAVIGPEDPLNNGVADALAKINIPAVGPTKSLAR
                     LETSKSFTRNLVSKYNIPGNPQFKVFKTLDGVEDFLNQLEGIVLKPDGLTGGKGVQVQ
                     GDHFDSKKEALKLCAQILAGGASLVIEEKFDGEEFSLQCLCDGKTVVATPLVQDHKRR
                     FDGDKGPNTGGMGSYSLADHSMPFLRPQDLHDGLEITRQVAEVLLKETGSPYKGVMYG
                     GFIATKDGVKLLEYNARFGDPEAMNILPLLKTDFVDVCRHIIAGTLDQIEIEFDKKAT
                     VCKYVVPKGYGLPADHPDAASSRAKIEVGNVGRARLYYSSVDQKDDGLYLSSSRAIGI
                     VGIADNLADARKIAEEGVKAVKGPVAYREDIGTDALIQKRIDHMKKIRKDA"
     assembly_gap    47007..47106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47148..48416
                     /locus_tag="PLR13_01265"
     CDS             47148..48416
                     /locus_tag="PLR13_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQC17985.1"
                     /translation="MGWNSDIVVLILLFTLSGFFSSSEAALLSLRDLHLHKMQQNNYP
                     FFNYVVKLLSNQRRLLITIVTSNEVVNVSISILAASFFIGLFGSSGQWVSIVFTTVVL
                     LFVGEAIPKTFGVTYPMQISSLVSPLLMAISRIESPIVSALEKVSGLFLRRYGVNKSE
                     ESEVIMEHEFKTLVDVGSLEGVVNEAQKELINKIFELGDRPVTDIMVPRVDMFCLPLD
                     MPVGEMLQSIAAARQERVPVYREDRDDIVGILFARDLLNNVWRGRSQTGIYNLLVRPF
                     FVPEQKTINGLLHDFQKNFIQIAIVVDEYGGVSGMITLEHILEHLFEEPEGDEEMVCD
                     GCEKIDEQSVIVPGSMSVEQCNKLLEAELPEDEFDTVGGLVLHLFGKIPQKGEEAHLD
                     GYIFCVHDVGKTRILKVRITKKTVEEPEES"
     gene            48413..49693
                     /locus_tag="PLR13_01270"
     CDS             48413..49693
                     /locus_tag="PLR13_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQC17986.1"
                     /translation="MSLYLIVAAILFCLMLEMLYSGGEVALFASDINKLKNRAHQGST
                     SAHQAVKLKEKPEWFISTALVGTNLAIIIASTLITGLLISFYGPARGEKIAFLIMIPT
                     LFLMIIARSIFLYYAEAMAMRVAWFIRFSSILFYPIAFVIAAVSRSTVHFSSDTRSGQ
                     SSHITKEGLKYILGEKTKGGDILTREKEMVSRVFDFSELTAMKIMVPVSALTALSASM
                     KISEAVGVVADKKYMRIPVYEDTVYNIVGILRYFDLFEMLLKDKEKGEGVGEATVASC
                     MRKDVFYVPETKKVSELLAAMQKRDEQMAVVVDEYGGATGVVTIEDIGEEIVGTIDEY
                     VSGEKLYRKIAEGRYLVSGRLEIGTLRQLVKAKFPEGNYETVGGFLMYQMGKIPRIKE
                     YYQYGDVTFIIENADQKSIKEVTVIAPFIQEGNK"
     gene            49811..51010
                     /locus_tag="PLR13_01275"
     CDS             49811..51010
                     /locus_tag="PLR13_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17987.1"
                     /translation="MKSDLTRQDWEEYLIRLYFGTKNDLIKSCINRAYRDFNRTLHGM
                     DDHKNKQDLFDRAVKYLLSIFIDIKKESLNDHEEFDKWHKEKCLHLKNLYKENDFINF
                     SIGQAQKWINMTFKYIFCVGEKRLPGYSGLYEFCHVPIDNIILDKIKKRGAPVPSKPW
                     SRLGDYNEYISLQKWFREQYKLPLDGEFILWMEEPHDNVIMPVQNNIVLDKTRVKEAK
                     DETIGPYQLNRIILCSADASPASQKKDKYKAEYFFPSAKWVGAVRNVASDLGCKFVIL
                     TTKHGLVNPSDDIDHYDLEIKGNENIINEKWQDTTSRLIRRGQYDILVFYAGGCPRDE
                     YLRILSPILKNLEIDLLTFGKPNMGDAGKIKDIVESLGEEASIDTLKKKLKFPDRLLF
                     FPHNNKA"
     gene            51199..51780
                     /locus_tag="PLR13_01280"
     CDS             51199..51780
                     /locus_tag="PLR13_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HQC17988.1"
                     /translation="MAELQELSRKTCFTLEDVAQSLGMQTASARVLCSRYVRQGVLIR
                     LKKGFYTTAWKWENLNRQDFFKIANILQVPSYISLMSALAYYEVTTQAQNNYQENICL
                     KRSIVYNVREAVFNYVKVQARYYGDFVKTDGIFIATKEKAFVDAAYLYSFGKYKFDVD
                     SLDMKKLDIKKLRSIIKIYPQKTKETVKRLCWI"
     gene            51768..52430
                     /locus_tag="PLR13_01285"
     CDS             51768..52430
                     /locus_tag="PLR13_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQC17989.1"
                     /translation="MLDLIQHEQFELEVLNRLQSGRFLANLIFGGGTMLRLCHGLNRY
                     SVDLDFWVVREMDWAKYYSRMEKYLGQFYKIADSANKHFTILFELKSPQFPRSLKIEI
                     RKTVKNVKTETSIAYSPHAPVQVMLKTVALKDMMTSKIEAFLDRREIRDAYDIEFLVK
                     RGIVLNADHEVITQMLTKLGKLGKKDFSVKLGSLLDVSERKYYVENGFRILEANLRDK
                     LR"
     gene            52492..53232
                     /locus_tag="PLR13_01290"
     CDS             52492..53232
                     /locus_tag="PLR13_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQC17990.1"
                     /translation="MNETKFCALILAAGKGTRMKSDKAKVLHELNGKPLLYYSIEAAR
                     AVGVEKIVAVIGHQAERVRETFAANGCIFVEQNPQLGTGHAVLQAKDVLTDYKGFTVI
                     LCGDVPLLKPATIKSLVDNHVAEKAVVSVLTTIPPHPHAYGRIVKDSKGNVLKIVEHR
                     DATEEEKKIGEINTGIYCVDTEFLFHALGKVTNNNRQQEYYLTDIVEIAVCEGQKVKS
                     FIADDYVEVMGINTLEELTRAEKYLRKG"
     gene            53447..54436
                     /gene="dusB"
                     /locus_tag="PLR13_01295"
     CDS             53447..54436
                     /gene="dusB"
                     /locus_tag="PLR13_01295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQC17991.1"
                     /translation="MKNINNIIEAVAKKGALLAPLAGISDLPFRLIARSQGCSLAYTE
                     MISSNGLVRNTRKTYEYLQTCPEDRPLGAQIFGADPQVMAGAARIVADTGVDLIDINM
                     GCPVKKVIKTGAGAILMKDPDLAARIIKAVKKAVIIPVTVKIRSGWNQSSINAVQIAK
                     IAEDSGADAITIHGRTADQGYSGHADWKIIAEVKKAVKTLVIGNGDIRQPQDAVQMLA
                     ETDCDAVMVGRAALGNPWIFKGISQALRGGEIECHPSLNQRREMIENHWKMEARMLGV
                     KLAGKSFRKHILWYTKGLDNSHRFREMAGKVNNEQEIFEALNNYFHLLNTEKI"
     gene            54492..54767
                     /gene="zapB"
                     /locus_tag="PLR13_01300"
     CDS             54492..54767
                     /gene="zapB"
                     /locus_tag="PLR13_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HQC17992.1"
                     /translation="MENHTLFNEGKVNSTVELEKFSKLEEKIKAIVEEKVSLKNQIKT
                     LEDTLKNKEAEIEKLNGKIKVLDEERNSVRARVDSLLDLIADVNSAK"
     gene            54786..55064
                     /locus_tag="PLR13_01305"
     CDS             54786..55064
                     /locus_tag="PLR13_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQC17993.1"
                     /translation="MKNSYNITILGQELSVLSDSEDEQVAEVVQFLNKKVDEILHSGN
                     GLKTLNVAILAALNISEEFLKLKKVNKELCDKLENRAEELIQLIEETS"
     gene            55078..55275
                     /gene="ssrS"
                     /locus_tag="PLR13_01310"
     ncRNA           55078..55275
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLR13_01310"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            55479..57047
                     /gene="rny"
                     /locus_tag="PLR13_01315"
     CDS             55479..57047
                     /gene="rny"
                     /locus_tag="PLR13_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQC17994.1"
                     /translation="MFDASFLSVLVLVVAVVSGIIIGFILKQSIAAKEIRSSKNLMAR
                     MAEEAKKEAETIKKEAVLQAKENLLKMKAEFDAESKERRNDFEAREKRIRTKEENLDK
                     KTDALAQKESSVESREKSISSKEQSLEDKRRKLDAALHEQQERLEKIAGISPEEAKNL
                     LIKSMEAEAKQDAAALVRKIEDDAKLTADKKAQEIISYAIQRYAGDVVAEKTVSAVSL
                     PSEEMKGRIIGREGRNIRAIEAATGIDLIIDDTPEAVVLSSFDPVRREVARISLERLI
                     QDGRIHPGRIEDIVKKVRTEVEQIVRETGEKASFDAGVHDIHPEIINLLGSLKYRTSY
                     SQNVLQHSIDVAYLTGIMASELKMNVREAKRAGLLHDIGKAIDHKVEGTHAAIGADYA
                     KRFGENPRIVQAIATHHDDGRNNTLLGVLVQAADTLSAARPGARREMLETYVKRLEEL
                     EKIANSFNGVDKCFAIQAGREIRILVENEKISENDATMLCKDIVKKIENELTYPGQIK
                     VTVIRETRVSDFAK"
     gene            57078..57866
                     /locus_tag="PLR13_01320"
     CDS             57078..57866
                     /locus_tag="PLR13_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQC17995.1"
                     /translation="MKILFIGDIVGSPGRMAVRELLPDLKARKKIDFVIANCENGAAG
                     FGVTKKIMEELFRDDIDVLTSGNHIWNKREVLEFIGESDRLLRPANYPATAPGAGAVL
                     VPLKTGGYVGVLNLIGRIFMQAVDCPFITAKNKVAELREKTKVIIVDMHAEATSEKKA
                     LGWYLDGEVSAVLGTHTHVQTADEEILPNGTAYISDAGMTGPFDSVIGIRKEAIIERF
                     LTQMPNKFDLAKDDLRMQGVIMDIDVDSGKAKSIDRISVKLKDS"
     gene            57880..59163
                     /gene="tyrS"
                     /locus_tag="PLR13_01325"
     CDS             57880..59163
                     /gene="tyrS"
                     /locus_tag="PLR13_01325"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQC17996.1"
                     /translation="MKNAYDILKERGFVEQITDEGLIQELFAKGPVTCYIGFDPTATS
                     LHIGSLVPIMSLAHMQQSGNKPIALVGGGTGLIGDPSGKTEMRQVLTREQIDYNANCI
                     KQQLSKYLDFSDGKALLLNNADWLTKLNYIDFLRDVGRHFSVNRMLAAESYKIRMEKG
                     LNFIEFNYMVLQAYDFFHLFQNYGCALQMGGNDQWGNMLAGTELIRKIAAKDAHAVTF
                     PLITTSLGQKMGKTEKGTVWLDGALTSPYEYYQYWVNCDDADVERFLKLFTFLPLDEI
                     SAVKNLKDAQLNMAKTVLAFEATKVTHGAKAAEDAWRASAEAFHSRPVETGMFPSCNI
                     PRTAAASDTSAIPRYKIPRKDLKSGVQICGALAKAALTQSISETKRLIEQGGIYINDR
                     QVKSIDEKLTLADFGDAEEIRVRKGKKKYLIVEIE"
     gene            59215..61305
                     /locus_tag="PLR13_01330"
     CDS             59215..61305
                     /locus_tag="PLR13_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQC17997.1"
                     /translation="MQKKIERKKKPFHYRHANLRRKSKSNSHAAFYRPEIVPALKSTL
                     SRIGKPYPAPFVPDEFQVKALEAIKKTDCLVIAPTGSGKTWIAREAILSVLEKGGRAW
                     YASPLKALSNSKWIEFGLHFDTENVGIITGDTKENTEAPIIVGTTEILRNQLYDAMHQ
                     GKDLNCDLVILDEAHFLGDTDRGVVWEEIMIYLPARIHLLLLSATIGNGEEIAHWLES
                     IRKKSCAVVAEDKRPVPLYPLFLHPSGCLHPFLEGKKVSPPVADFIRRTDEKRLRGGK
                     MPDYIGIIRVLEKFNLLPAIFFLKSRAECDAALTARGMLQPLQNATVFSYSLDELLGR
                     FPTLARHRQLKVLRSMGLASHHGGQLPAWKMLVEEMMNRGHLRVIFATSTIAAGVNFP
                     ARTIVLFNSDLFNGSDFSPLTATEFRQMTGRAGRRGQDNIGFMLTVTGKFMNLNHIRG
                     LLFQKPEDILSRLKNDFAMVLNLLLSQTPDDVRKIFERSLAAYQQNSRHESADFSAAQ
                     SLWKDFSRHLKFLKQEGFVDESGALTDDGCWASKLRLDYPLLVAQCLRENAFPQDNEK
                     ILAALVALLAYDRDNDMKMTVKDLPPKLALAFKKVMLAVRPLIHRMENAGFAPARLHT
                     LAGVAMYLWAQGYGWDSVIKATGIAEGDMATLVLRTADNLRQIASLKDTYPDIAGCAQ
                     RARETILREPVLFL"
     gene            61665..61982
                     /locus_tag="PLR13_01335"
     CDS             61665..61982
                     /locus_tag="PLR13_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC17998.1"
                     /translation="MWLYLKTGFYSVVTKEPCKEDELLVRAHSKDDLDKLQDLLKKQC
                     QFHGEVLDTPQTDYAYRMIVPKETFASFMSRAINDLVYEEYKNTKSWEAAYRWQSNLQ
                     RSK"
     gene            62038..64032
                     /gene="uvrB"
                     /locus_tag="PLR13_01340"
     CDS             62038..64032
                     /gene="uvrB"
                     /locus_tag="PLR13_01340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQC17999.1"
                     /translation="MKKLSMITDFVPAGDQPQAIDRLVQGLEKGNEHQVLLGVTGSGK
                     TFTIANVIERVQRPALIIAHNKTLAAQLYEEFKGLFPENAVEYFVSYYDYYQPEAYLP
                     VTDTYIEKDSAINEEIDKLRHAATHALLTRRDVIIVASVSCIYGLGSPEAYLGMIVQL
                     EAGKEFSRDELLKRLIEIQYERNDIDFHRGTFRVRGDIVEIFPPYEDEQALRIEFFGD
                     MIESMTLIDPLRSKKIGVIHKTAVYPGSHYVSSRENLQRAIHDIRRELALTLAEYRDQ
                     NKLLETQRLEQRTNFDLEMMEEMGYCQGIENYSRHLTGRKPGQPPPTLMEYLPQDALI
                     IIDESHATLPQLAGMYRGDRSRKETLVKYGFRLPSALDNRPLRFDEYEALPNQRIYVS
                     ATPAQYEMAKAKGIRVEQIIRPTGLMDPEIEVKPATNQVDDLLAEIRARIEKNEKVLV
                     TTLTKRMAENLTDYYAGLDIKVRYLHSDVHTLERVDIIRDLRRGVFDVLIGVNLLREG
                     LDIPEVSLVAILDADKEGFLRSERSLIQTSGRAARNVSGKVIMYADKMTHSIQACLDE
                     TKRRRAIQQKYNEENNITPETIKKSISSVLGSIYEADYVTLPATVSERVAPLKEEDLP
                     AFIAKLTREMKQAAKNMEFEHAAELRDEIRELNKILLEMG"
     gene            64147..64605
                     /locus_tag="PLR13_01345"
     CDS             64147..64605
                     /locus_tag="PLR13_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18000.1"
                     /translation="MKALKSILCVLTLFVFVFAGCEKEASDWVQYHKDADGNVYWYDQ
                     NRVKVDAANYTVEVWAKQVYSDMGRTIELQSRIKDGLSMKGYDKLAHKTCRYAIDCGK
                     KGITVLSISHFDSDGESLYFDVDKGEKKMFIINPGSPAAALQKEVCSKKQ"
     gene            64702..65100
                     /locus_tag="PLR13_01350"
     CDS             64702..65100
                     /locus_tag="PLR13_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18001.1"
                     /translation="MNKVVARLANGKMIKGTTADFFPGKDVFHVTESAAAPGTKALEV
                     FTKDMKALFFVKDLAGNPQHVKTNEFNASQPVVGRKIKVVFKDGEVLIGTTTGYQPGR
                     PGFFLVPADAGSNNERCYVVTASAKEISFL"
     gene            65179..65415
                     /locus_tag="PLR13_01355"
     CDS             65179..65415
                     /locus_tag="PLR13_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HQC18002.1"
                     /translation="MPQKQKKKKRDIEKDYAAGQFVMKLRRLADCIETGHPFRIQIAG
                     EKITIPTDAVINIEHERGTSEEEVEFQIKWKLNR"
     gene            65479..66498
                     /locus_tag="PLR13_01360"
     CDS             65479..66498
                     /locus_tag="PLR13_01360"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQC18003.1"
                     /translation="MGCFERRETHTIMLDDIYHGYLDWNMMFAAERLGRLDAQKTGIA
                     AYLARDSYYNDPRYAETLLTLGFFDVQLFYHNHLQALIGCRGIQTPEGAKALVVIVFR
                     GSDQVQDWLQNLDIRMTRFLNKEKFPHFSRLKVHNGIFERVHDFEDWSRRVIFKDGPL
                     KGNLLQMLGDELKRKQCFFWIIGHSLGGAMATLYAARLCDCYNVSRNRILSHTFGAPP
                     IGNRYFAARYGHESCLRAQKIKRKSDSLALFRFVNTEDPIPAPRFADVRVEKAQGGPI
                     AQPPYRLLGFKHVGKEVRFSPRHLPGFYEKYKQFTGRRYNSSHFMKVHFMEAYITGID
                     VLNTH"
     gene            66710..67009
                     /locus_tag="PLR13_01365"
     CDS             66710..67009
                     /locus_tag="PLR13_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18004.1"
                     /translation="MPFEMMKKVMLTGIGLALKTRSEMETVAKDIIKKSKMSEAEGRK
                     FVADLTKKYEQSRRDMEKTIQKGIADYMASADIASRKELNALKKEIGNLKKARKK"
     gene            67077..68774
                     /locus_tag="PLR13_01370"
     CDS             67077..68774
                     /locus_tag="PLR13_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HQC18005.1"
                     /translation="MLSIKKIGVLGRTYRNFSRYRQILSILFRYGFDDLVERLKIDQY
                     IEIGLQMISRHQRENVERLSRPERVRLLFEELGPTFIKFAQILSTRPDLVPADVIQEF
                     EKLQDNVPPFPFSEAKAIIESELGGDLDNFFLSFEEKPLASASIAQVHKAVLNDGEVV
                     AVKIQRPGLKKIIEVDLEIMLHLASLMEKNIEEISFQKPTRVVEEFARTLERELDFTT
                     EASSMERVAGQFLSDRTIYIPKVFSELSGRRVLTMEYVDGIKVSDMEKLEKAGMDLKK
                     ITSRGAHFVIKQVFEFGFFHADPHPGNVFVLPNNVICVLDFGMTGSVDKRQRALFVDI
                     METLGRKDAEQCARLMLELGEYDDEPDIRLLEREIDDFMGRHLFKSLKDIDLARLIQD
                     LLDIATSNKIRIPPVIFLMMKAFAAMEGIARLLDPEFDMIAYAEPFIKRAKLARYAPE
                     KIAADLLTVVSESMHMIQALPREVLQVTRLIRQNKMTINMEMRWIDTFLRDLDQAGNR
                     LSFSIIIAALIIGSAYLLAYSTPPLVYGISLIGLIGFSAAAVLGGWLLIAILKKGML"
     gene            complement(68840..69286)
                     /locus_tag="PLR13_01375"
     CDS             complement(68840..69286)
                     /locus_tag="PLR13_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQC18006.1"
                     /translation="MFAPKRILVPTDFSDYSDEALKQALELAKQYHSKVYLLHVANPV
                     TQCTADYCLDVDTFKKAEESTMIKARDMMQQELQKFPESQEIEIISDIREGEPVAEIL
                     KEQEEKGVDLIVMPSHGRSGLLKRLMGQIAEKVMEATKTPVLLVHH"
     gene            69646..70968
                     /locus_tag="PLR13_01380"
     CDS             69646..70968
                     /locus_tag="PLR13_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQC18007.1"
                     /translation="MKNYKILIIGSGPAGLFAALRLEKLGMDDIAVIDRNPYPAGGLL
                     NDGKLNFDYRVGLDIDELQIDRVTAEGMMLEIKRIFTGFPRCRQVTFTDNRNNLRALA
                     DMAAEHGAQFIAPEQWHWGTDNGRALVDYLRGQLKKTDFLLSTEVESVEKKREGSFRL
                     LCRIDKKRKSCCAPVILAAPGRSGAYWFRDIARALDIRNNFGPIDVGIRLELNRKFYD
                     FVTGIVYDPKFIFTTGRHGDKVRTFCTNPGGRVRVESYGDFKLVNGDALSGKKTKNTN
                     FALINTVALTKPYSDTTEFGLSIAKQFFLLGGGKPIVQRIGDFREGRRSSLSTFNSTA
                     RHFEVCRATCSATPGDITLAMPARIMDNLWESLKSLDKIIPGILHPSTLMYAPEIKFF
                     DTHFPTDHYLETNVKGIFVAGDGTGKSRGIVGAAMSGMIAAEGIMNGR"
     gene            71062..71232
                     /locus_tag="PLR13_01385"
     CDS             71062..71232
                     /locus_tag="PLR13_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18008.1"
                     /translation="MDKVANEIGHYIIRAFDAMGAEIIGDVFRWSGVTETGYGAIFIV
                     LAILVVIYIVKK"
     gene            71270..71767
                     /gene="lepB"
                     /locus_tag="PLR13_01390"
     CDS             71270..71767
                     /gene="lepB"
                     /locus_tag="PLR13_01390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQC18009.1"
                     /translation="MNKTAIRRFFFPDITARYVLRLAAVAAVAFILFKYVFIPFRIQG
                     ESMAPTYVTGSFNFCFALRYMFASPAPPDVVTVRMAGQRIMLLKRVVAVSGQRVEFSK
                     GILFVDDKKINEPYVKKKSNWNLEPIIVKPDHVYVVGDNRAMPAEAHTFGQAPVSRIV
                     GVPLW"
     gene            71761..72261
                     /locus_tag="PLR13_01395"
     CDS             71761..72261
                     /locus_tag="PLR13_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18010.1"
                     /translation="MVKRKYLAMGLILVSAFAVYFLFFVDWEARAVKKHLQALAKELE
                     WSTGENELAVAHRIRRIQEGMAENCQVDIPSYGISRAVVRHDVPVYLMTARGYYKNLS
                     VELEDLEIETFELPAAQAVATAYVKAIQADGVLNDEVLVIRFRLQKVNKKWQVNEAGE
                     VHVLEK"
     gene            72327..72761
                     /locus_tag="PLR13_01400"
     CDS             72327..72761
                     /locus_tag="PLR13_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18011.1"
                     /translation="MQKRNDRQPYSLESIVELYPRLYSQKRLDEWMDLFDKRAIMVRV
                     QKGNPVSFVSITEGITEQREYAAENALFDEKWHHVKINRHGNIAVIKANYILTAEREI
                     RKGIDVLTLCCRDGGWRIICLAYEQKEMVRRSIPRKDSVKTS"
     gene            72944..73159
                     /locus_tag="PLR13_01405"
     CDS             72944..73159
                     /locus_tag="PLR13_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18012.1"
                     /translation="MNEEIDDDLIDIELEGDALEKGMRKLESEAERLLHYTNDLLQEL
                     NKDRAKREAMKQEDEKTQSTEEGSASS"
     gene            73546..74439
                     /locus_tag="PLR13_01410"
     CDS             73546..74439
                     /locus_tag="PLR13_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18013.1"
                     /translation="MAMKVLNNRNFIFILAMILGLFLPQPAEWTKVLMIPALALVMTL
                     ATVNVPNNYFLNPRAILKPSLAGMFMSYIILAGVLLCLSALLIEDKNLWIGFVLIASV
                     PPAVAVIPFTAILEGNVDYTLSGTVASYIAALLIMPVILWIFIGTVFEDPYKLLQIML
                     LLIVLPLILSRLILYFNWQEKLSSVRGFLTDWGFFIVLYSMIGVNRDLIFSNPLTVFS
                     VAVISFCTTFVLGWFIQRICAFCRIDKKNMVSLLLLGTLKNQGIAGGLAITLFEKEAA
                     IPSAIYSIFMIVYIMWLDWRK"
     gene            74711..75220
                     /locus_tag="PLR13_01415"
     CDS             74711..75220
                     /locus_tag="PLR13_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18014.1"
                     /translation="MIIFSGSLAMGETSTAADWFNRADGLWDGKKFIEVKEAMAYLDK
                     AIEQQPNYANAYISRGMAYNQTGDYQRAIQDYNLALLFTNDENSLTKIFDKMGVAYAN
                     LGQYQRAINNFNHAISLKPDYADAYNNRGIVYFLQGDKKQCCNDAQKACELKNCELLS
                     WARERGACP"
     gene            75787..76272
                     /locus_tag="PLR13_01420"
     CDS             75787..76272
                     /locus_tag="PLR13_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HQC18015.1"
                     /translation="MKKITFTMIAVFLGLLLTSQAFAWGPGPGKKSGYCRGVVLEELN
                     VTEDQKTKLDALQLQHFKAVHPLREKIRDKSAALRKLWLQVDPDKNKIMAKQKEVRVL
                     RDQLEDNKTAYRFEVNKILTPKQKEKLAKCGWNKKSGFGPRGGKRGEQWNGHGNCLYL
                     P"
     gene            76351..76932
                     /locus_tag="PLR13_01425"
     CDS             76351..76932
                     /locus_tag="PLR13_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18016.1"
                     /translation="MKNKVYKALLLTLIVLMPVSAMADVRVHVNIPLPLPVVFPSPPH
                     LVVIPDTDVYAVADIGEDIFFHAGWWWRPWNNRWYRSRYYDRDWAYHPYQPSFQRYVP
                     ADWRQNYRHKTWNGYRWEPQRRSYQDIQRNWHVWEKNRHWDKQSYGVKKITKRAPAPQ
                     YRSNTRPAMTKPAMQHNVKSRGDFRTSERRDRR"
     gene            77158..78390
                     /locus_tag="PLR13_01430"
     CDS             77158..78390
                     /locus_tag="PLR13_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQC18017.1"
                     /translation="MEDIKITPADVQETLCFTGNIIEESGARLAGSESCKKAAVLIMN
                     EMNKHCDSVSMEEFDIHPKAFLGFFKVVVVIYILSSFLLYFDYVVAGAAGYLLGAFIM
                     LGESIFYWEMLDPFYRKMKGYNVIGTIEPEGEVKQQIILSGHHDSAHEFRFLAHHQKL
                     YAVRIMTAVIIIFAALVLSWIWVYHRSYSGADPAFVPYLRYGVFAGLIFVAPMYFFIS
                     EKGTPGAGDNMIAVAAIIKLAEIFNRARQNGAGLLKNTRLMFLSADAEEAGLRGARAF
                     VKRHRGALLKMPTYHFNIDSIYNLGELQFLTRDINSVVRLSKNMARECKAVADQLGYR
                     TRLFAITPGGGATDAAEFAKAGVEATTLIGMPTNLIRDGMVYHTLQDNVEHIEPEAVE
                     ATLKIAFVYILNKERDNI"
     gene            78553..78753
                     /locus_tag="PLR13_01435"
     CDS             78553..78753
                     /locus_tag="PLR13_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18018.1"
                     /translation="MTKNEISREEIIKKLVDSDLDGLSQEKSIKFLVRILHNGWKGYN
                     EMSDTELLEAFNHREFRKTVRT"
     gene            79039..>80749
                     /locus_tag="PLR13_01440"
     CDS             79039..>80749
                     /locus_tag="PLR13_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQC18019.1"
                     /translation="MEQQNRQSSENEEQYGSLLEAIEEGYYEVDLAGNFTFFNKAMCR
                     ILGYAPDEMRGMNNRQFTDKENAKKLFETFNRVYKTGESTKAFDWQLIRKDGNKKYIE
                     TSVSLIKDASGKPTGFKGIVHDITERKQAEEALRESEALYRLLADNISEHVWVMDLNL
                     NLIYISPSVEKIYGYALDEISQIKMRQLFTADSYRKVVETFMKELEKAMNSKPPPTGT
                     NYIMELEAYHKDGHKIWIENRLHFIRDKNGKPVSLLGETRDITDRKLAQEALKASEEK
                     YRLLADHMKDQVWMMDMNMQITYVSPSVERITGYSFEEIKILPLDQLLTPDSVRKATE
                     FITNRMPKAFNLVSLDSQYRTLELEFRTKEGKTIWGECSFNFIRNENNKPVSILGEAR
                     NITDRKIAEDKLRQSEEKYRTILEDIKEGYFETDLFGNFTFFNDTVCRVLGYSRNELT
                     GMSNKIYTDAEERKKVYRAFHQVYMTGEPYRDLEWQIRGKDGTSKYIEGFISLLRDSS
                     GNPKGFRGIAHDITERKIADEKLQKTLESLKKAVGTTIQVLISALEARDPYTAGHQSR
                     TANL"
BASE COUNT        22112 a        17582 c        20240 g        20515 t
ORIGIN      
        1 ccttgtcggt tttttacgtt atttgcaagg agttgtcaag aatattttat aatttattta
       61 taattaaaac agtaattttc aaagaacaat cattgttgtt tgaagaagtc ataggtcaaa
      121 aaatttttac agatcgggtt cgtccgcccg gccggattca aacaaaaaag ccggtgcgtt
      181 tttacatgtt tttttacacg aaacggaccg gccttttttg aggatatttt aataatttat
      241 cggtatgtca gtttttttaa ggcttctatg cgatcctcaa gcggcggatg gctcatgaaa
      301 agaagtttga gcccgtggcc ggaaggcttg ccgttgatgc cgaaagccgc catctgatcg
      361 ggcagcggtt cagcggttcc tcttttgagc gcttcgagcg cgttgatcat gttttgcgtt
      421 ccggccagtt tggcgccgcc cgcatccgct ataaattccc gttttctgct gaaccacatc
      481 acgatcacac tggccaggat gccgaagacg atttgcgcga cgagggacgt gacgaaaaat
      541 ccgatgccgt ggccttcttc ttcatttttc agaatgaccc ggtccacaaa atgacccaca
      601 atgcgggaca ggaaaatgac gaaggtgttc accaccccct gaatcaggga cagggtgatc
      661 atgtcgccgt tggccacatg gcttatttca tggccgatga ccgcttcgac ttcttccttg
      721 ttcatcgcgt gaagcagtcc cgcgctgacg gccaccagcg ccttattctt gttcattccg
      781 gtggcgaaag cgttcgggga cggagagttg aaaatggcga cttccggcat gccgatattc
      841 gccatctgag catgtttttt cacggtgtcc accagccagg tttcagtcgc actttgggga
      901 ttggtgatga cttgcgcgcc catcatgcgt ttggccatcc acttggacat ggccagcgat
      961 ataaaagcgc ctccgaaacc gaataccgcc gcgaagacga gcaggttaac catattaaga
     1021 ttcatgccgc tttcgtctag aatacggtcg acaccgagca gacgcaaaac aatgctcaaa
     1081 acgacaagca cagcgatatt ggtcgcgaga aacaacaaaa ttcttttcat ttttaacctc
     1141 ctgagttaaa aactgccccg ggcagccatt gacggtacgt cttgacaatt ctttttgtta
     1201 ttttaatggc aagaaccggg atgtcaagtc tttatcgtga aaaagtcccg gattgttttt
     1261 ctccggaaga tgtttttgcg ggattgggcg gaaggaatcg gattatgggc gctttgattg
     1321 tcgctggtat tgttttttcc ccaaggggac ggactcaacc ggcaccccga atttgcttga
     1381 cacagacaac tatcttgttt caagatatct gcacattcaa tcattattca aagcggccaa
     1441 ccaggaggaa tcacaatgaa gaaaaaagat atcgtacagt atcctcagac tatcagcggt
     1501 tttgaatttt ttgtcaagtt catggcgttc gttggacgga ttccatttgt cgtctatcac
     1561 gggatcaagg ggatcaaatc gattagcagc aatcgcaatg cctcctgggg atcgcttctg
     1621 caaaaatccg cactcaagta tccgaacaac ccggcggtga aatcacccga cggcacattg
     1681 acctatgccg aacttaatga gaaggccaac cagtttgccc attttctgct gtcgaaaaac
     1741 gttaaaaaag gcgacacggt tgcagtatgt cttgaaaaca ggccggaact gctttttctt
     1801 tattgcggat gcgcaaaagt gggggctgta tgctctctca tcaatacaaa tcagagaggg
     1861 gattctctgg tacacagttt caatctcaac aaggggaagg tgatggtggt cggagaggag
     1921 tgctacagtt tcttcaagga cgtgaaggac cgaatcgacc ttcagggatc ggagctttgt
     1981 ttgctcaggg atccggcaat gaatcaatta aaggcgcccg aagacattac ggatcagctt
     2041 gccgtcctgc ccaaaaccaa tctttccgaa acgggcggga tcagcctgaa agatacgatg
     2101 gcctacgtct atacctcggg aacaaccggc gggttgccca aagcggcggt catcacccat
     2161 aagcgggcta tgtccgcgac attctggttc ggaagggtcg taacgcatgt caagccgaaa
     2221 cacaccatct acattcccct gccttttttc cacaccaatg ccatcaccgt gggatggccg
     2281 ccggccctgt acagcggagc ggcagttgcc atgaggcgga aattcagcgc gagcggcttt
     2341 ctcgacgacg tgaagaaatt ccaggccacg cattttgtct acgtgggaga agtgtgccgc
     2401 tacctgatgg cacagcccga aaaagccggc gaaaacaaga ccaccctgaa atacattatc
     2461 ggcaacggcc tgagaccgga tatctggatg gctttcaaga ggcgtttcgg catcagcaaa
     2521 atatacgaat tttacggagc ggccgaaggg gtcggcgtgt ttaccaatct tatgaatttt
     2581 gattataccg tgggaaccag tctcacgccc tttgccattg tccaatatga tatcgaaaac
     2641 gattgtccgg cgcgcgatgc cgggggcttc cttatcaaag tcgccaaagg cgaccccggc
     2701 ctcctgatta tggaaatatc tgaaaaaacc ccgttccccg gttataccga caaaaagaaa
     2761 accgaagaga aaattatccg caatgccttc aggaaaggcg atctctggtt caataccggc
     2821 gacatgcttc gcaatatggg atttcgtcac gcgcagtttg tcgatcgcct cggcgatacc
     2881 ttccgctgga agggcgaaaa cgtcgcaacc atggaagtgg aaaaagcgct ggattctttt
     2941 tccggcgtgt ccgcttcggc ggtttatggc gtggttatgc cgaaaggcga cggcagagcc
     3001 ggtatggccg ccgtgataaa acaggaaggg aaagatgttg attttgccgc gctggcagag
     3061 cacctccggt ccgttctgcc caaatacgcg gtgccgattt ttatccgttt tgtcaatgat
     3121 ttccagtgga cggcaacgca taagatcaag aagaacgatc tgaaaaatga cggatataat
     3181 ctgtcggcga tcaaagacgg cctgtatgtt ctcctgcccg gaaccgaaac atacgttccc
     3241 ctgaatgacg ccatctatca ggacatcatg gccggaaaat ataaatttta agagacttgt
     3301 ctgtagccgt ttccatggca ggtgggttcg cctgaccata tggagacatt gagattgttc
     3361 ggcttatcgg gttattcaat ctgtctgtga cgcctggatc tcatgaagtt tgcttgcaag
     3421 tgtcggaata atatggtagt tcacttcaac ttaaccaggg cgcctgcaga ggttgtcctg
     3481 gttttagatt gtaaaaagat tattcgtgac gtgatcactt aaatacagga gcaaacaatg
     3541 gattacaaaa agaccctgaa tcttccccag acggattttc cgatgaaagc gaatctggcg
     3601 cagcgcgagc cggaaatgct gaaaaaatgg gaagacatga acatctataa aaagatcaga
     3661 caaatggcca aggggaaaac gccttacatt ttgcatgacg gtcctcctta tgcaaacggc
     3721 aatattcatc tgggcaccgc tctcaataaa atcataaagg acattgtcat taaatcaaaa
     3781 aacatgaccg gatttgacgg tgtctatgtg ccgggctggg attgtcacgg cctgccgatt
     3841 gagcatcagg tggataagga actgggcgcc aaaaaggggg cgatgtcgca ggtggaaaag
     3901 cgccgcgcct gcaggaccta tgcggaaaaa tatgtcgaca ttcagcgtga acagttcaag
     3961 cgtttcggtg ttttcggcga gtgggagaat ccttatctga cgatggccta tccttacgaa
     4021 gcggtcacgg ttgaggaatt tgggaagctt tatctcaacg gcagtgttta taaaggaaaa
     4081 aaaccggttt actggtgcgc cacctgcaga accgcgctgg cggaagccga agtggaatat
     4141 gccgatcatc acacgccgtc catttacgtg aaatttaatt ttgtctccga cattgcccag
     4201 gtgctgccca aactggccgc ccagaaagtg gcgatggtca tctggaccac gacgccctgg
     4261 acgatcccgg ccaatctggc gattgccgtc aaggatgatt ttatttacgt ggcggtgaaa
     4321 atcaatgacg acgtaatgat tgtcgctaaa gacatgttgg attattgtct ggacgccttt
     4381 ggatatcagg gcaagtccta tgaaattatc gatgagttta agggcgcggt tctggaaggt
     4441 cagaaatgcg ttcatccgct gattaagaga aacagtctgt tgatcctggc gccgtttgtc
     4501 acgctggatg ccggaaccgg cgccgtgcat attgctcccg gccacggtca ggaagactat
     4561 gaaatcggcc tcgagtacgg cctggataat tacgcgccgg tggacgacgc gggaaaattc
     4621 acggaagatg tcgaacattt tgccggacag tttgttttcg acgccaacga cagcgtcatc
     4681 aagaaactcg atgaagtcgg cgcgcttctg gggcatgtgc cgatgcagca ttcctatccg
     4741 cactgctggc gctgcaagaa acccatcatt tttcgttcca cggaacagtg gttcatttcg
     4801 atggagaaaa acgatctgcg caagaaagcg ctggaatgca ttaacgaagt cacatggatt
     4861 ccctcgtggg ggaaagaccg catctacggc atggtggaaa accgcccgga ctggtgtatc
     4921 tcgcgccaga gattgtgggg cgtgccgatt accgtgttct attgcgccaa atgcaaaaat
     4981 gaagtgatga ccagggaaat gatggatcat ctggtggcgc tggtggaaaa aaacggagcg
     5041 gatgtctggt ttgaaaaaga tccaaaagac ctgatgccga agcatacgac ctgtccgcac
     5101 tgcaaaggga cggagtttac caaggaagtg aatattctgg atgtctggtt cgattcggga
     5161 gtcagccacg cggccgttct ggaaaaacgt ccggatctga gttcgccctg cgacatgtat
     5221 ctggaaggca acgatcagca tcgcggctgg tttcattcgt ccctgctgga aagcgtgggt
     5281 acgcgcggac gcgcgccgta taagaatgtt ctgacacacg gctttgtcgt ggacggggaa
     5341 ggcaaaaaaa tgtccaaatc cgtcggcaac gtcatcggcg cggaagaggc gatcaataaa
     5401 tacggcgcgg aaatcctgcg gctgtgggtg gcggcggaag actacaccga cgacatccgc
     5461 atttccgaag aaatcctcaa acgcctgatg gaagcctatc gccggatccg caacaccagc
     5521 cgtttcattc tgggcaacct ctacgatttc aacatggata aggatgcggt ggcgtacgag
     5581 cagatgtccg agctggaccg gtgggccctg catcgtttgc aggaagtcat caaacgcgtg
     5641 acggaggctt atgaacggta tcagttccac gtggttttct acacgcttta caattattgc
     5701 accgttgatc tgagcgcgct ttatctcgat gttttgaagg accggctcta caccaacaag
     5761 gccgcttccg cggtgcgacg ctccggtcaa acggcgatgt ttatcattct taacgcgatg
     5821 accagattgc ttgcgccgat tctgaccttt acgtccgagg aagtctgggc ggcgatgccg
     5881 gactggacca acaaggaaga tagcgttcat ctggcgcagt tcccggaagt cagcggcacc
     5941 tttttcaatg cggacctggg cgagcgctgg aaagcgatga tcgacgcgaa gagtgaaatt
     6001 gccaaggccg ttgaacaggc gcgaaaagag aaaattgtcg gccattcgct ggatgcgcgc
     6061 atcacgattg cagcgccgga aaaactgcgg gcgttgtttg ccgcgcatct cgaggattta
     6121 aaagcgctgc tgattgtgtc gcagctgcag ctggccgatg agcaggatat tgccgcaccc
     6181 ttccggagtg cagaaattga agggctcatg gtcggtgttg agaaagcccg cggcgccaag
     6241 tgcgaacgct gctggattta tgacgagtcc gtcggtgccg acgcgcagca tccgaccatc
     6301 tgtgcccgct gtctgccgaa tctgtaaaaa aaggagttct ctttgaaaaa gaatattatc
     6361 atttttgttc tgggagcggc tgtcatcgtc gtgctggatc agatcacgaa agcggctatc
     6421 gtaaaaaaat tctttctgca tgaaacccgt tcggtgattg acggtttttt taatcttgtt
     6481 tacgtaatga atcccggcgc cgccttcggc tttctggccg gcatgtccga gatgtttcgc
     6541 tctctgtttt tcatcggcat gaccctgctg gtcatggctc tgatcattta ttacatcgcg
     6601 aaaagcaaat cggaaaacat gttcttcgtc gtttccctga ccatgatctt cgcgggcgcc
     6661 gtcggaaacc tgatcgaccg gattcgcttt ggagcggttg tggattttct tgatttttac
     6721 atcaaaacag cccactggcc ggcatttaat gtggctgatt cagccatcac catcggtgct
     6781 gtgttaatga tctggggtat ggtcgcggga cgaaaacgag aggctgtctg atcatcacgt
     6841 tttaaacgtt gagaagggta cggccttgca ctgtggattg caggtcacag cattttatgt
     6901 ggcggaaatt tacggttgtt ttgagtcttt gagtttctgc aattcttccc tgtagctttc
     6961 cgcctgagcc cggatggttt cgatttcctt ttgcgcgtgt tccagttttt cttcctgcag
     7021 gtgtctttcc tgcctgtttt tttcctggat ttcatcgatg cccacgtaaa cggccaatgc
     7081 tcctccgagc agcaggaaaa tcggaaggga tcctttaaaa atcgtcataa aatccggcca
     7141 gaagaaaatt aaactgacca atccgaagat agcggaaatg attcccccga tgagcaacga
     7201 catagtgttc cctccggtca agatgatgat ttgaagacat tttttttcta tcataaaaag
     7261 accattccgg caagatgcaa acagatgctg tgaaatgtga aaatattgct tgatttaaag
     7321 ctgttcatca tttaaacgta taaaatcata aagatatgag gtgaacatga tagagcctga
     7381 ttttgccaaa ggagacggtc tgattcccgc gattgtccaa gatgccgaaa cgaaagacgt
     7441 tctgatgatg gcttacatga atcgcgaatc gtgggacgcg actttaaaaa caggaaaggc
     7501 gacctattgg agccggtcgc ggaaaaaatt gtggttgaaa ggcgagtcct cgggcaatgt
     7561 tcagatcgtc aaagcggttt ttatcgactg cgatgaagat accattctgt tacatgtcca
     7621 acagatagga gatgccgcct gccataccgg tcaccggtcc tgtttttacc gcaagttgga
     7681 aggaaatgat tttgttgccg tcggagaaaa gatttttaat ccagaggatg tgtataaatg
     7741 aaaaatttaa aactgggaat tcccaagggc agtctggaag ccaataccat cgatttattt
     7801 aaaaaggcgg gatggcgcat caccagcgac gcccgaagct attttcccga tatcgatgat
     7861 ccggagattt cttgcaagct tgtccgggcg caggaaatgt cacgttacgt tgaagacgga
     7921 acgctggatc tgggcctgac gggtatcgac tggatcatgg aaaatgattc ggatgtcgtt
     7981 gtggtgcagg atcttattta ttccaaggtg tccacgcagc aggcccgctg ggttctggtt
     8041 gtccgtgacg attctcccta caaaaagatc gaggatatgg agggaaagag aatatccacg
     8101 gaattggtca actttacgaa gaaatatttt gcggaaagaa acatcaatat tcatgttgag
     8161 ttttcctggg gagccaccga agccaaggtt gtcgaggggc tggtggatgc cgttgtggaa
     8221 gtgacggaaa cgggctcgac aatcaaggcc aacaaattgc gcatcattca tgaactgatg
     8281 aaaaccaaca ctcagttaat cgccaatcgc gctgcgtgga aaaatgcatg gaaacgcaaa
     8341 aagattgagc agatcagcac catgctgacc ggttctttgc aggccatggg gaaggtgggt
     8401 ctgaaactta acgtgtccaa agaaaatctg aaaggcgtta tcggcctgat cccctcactg
     8461 aaatctccga cgatttccgg tctgtcttca gacaaatggt ttgccattga atcgattgtt
     8521 gacgcgaaag tcatgcggga tttgattccg aaacttctgg atgcaggcgc tcagggtatt
     8581 attgaatatc ctttaaacaa ggttatttag cggagacaac gattatggcg agaaaagcga
     8641 agattgtccg taagacaaca gagacggata ttcgtcttga aatcgatctg gataaaaccg
     8701 ccggcagcag cattgagacc accattccgt tttttaatca catgctggag ctgtttgcgc
     8761 gtcacgggtt tttgaaactg atcgtcagaa gcaaggggga cacgcatatt gacgatcatc
     8821 atctggtgga agatctgggc atctgtctgg gccaagcggt ggctaaagcg ctcggagaca
     8881 aaaaaaacat caatcgctac ggatcggccc gtgtgccgat ggatgaatgt ctgtgccagg
     8941 tggatatgga tatctgcgga aggccttatc tgatctatca cgtgaaaagc ggccggagaa
     9001 aaatcggcaa atttgatccg gcgctggtca aagaattttt caaggcgttt acggatcaaa
     9061 gcggcatcac gctgcatatc aattttctgt atggcgaaaa cattcaccac atgattgaat
     9121 cgatattcaa ggctttcggc cgtgctctca gaaatgccgt gtccctaaac gaaaacatca
     9181 agggcgttct ttccaccaaa ggaaagcttt agccggttgg attctgcttt tctgtggaaa
     9241 gcagcgtcat tcatacaccg atcccttatc ggcgggacct gtcccgttga tcgggagtca
     9301 agacatcgag gtgagatcat ggttgccatt ctccgcaaat atttttgttt tattgtgaga
     9361 tttgtttttg tcgctttgct ggcttttcct gtcatgtcgc aagcgctgga taaaaaagca
     9421 cagagcgatg aaagcgaaaa tctgatagcg gtcatcgcct ttcagccgtt aaggccggaa
     9481 ggcggcaccg gaaataccgt cctctgtccg atttgcggga aggcttcggc cggcggcatc
     9541 atacaggagg gggcggaaaa aatcgttgaa gaaatttttg tagaacaact gaaggcaaga
     9601 gacgatatcg tgttggtacc cgcggaaaaa ttacagaaag cgactcaaaa atattctacc
     9661 gacagcatga aagattttca agcaaccggc ctgctgaaga tcggcaggga attgaaggct
     9721 gatttcctgg ttgtcggtta tgtttaccgt tttgcggaaa gggtgggcta ccggtattca
     9781 tcggaacatc cggcgtctgt tgtttttgaa attcatttga ttaggactgc cgacggcaaa
     9841 tccgtctggc ggggatattt tgataaaacg caacgatctc tgatggaaaa tgttctggac
     9901 atctcttcgt ttttcaaggg cggcgcaaag tggatgacgg cccgcgaact gacggaactg
     9961 ggcatggaga acgttttcca gacgtttccg gattttcagc cttaatccat catacgcgga
    10021 tgcggcggat cgtgaaaccc ctccgtggcg ggttttagac aatgtgacga aaaaattaag
    10081 ccttgctgtt tttgtgacat gccgtgattt ccgactgaac cgttcagggc tcttcttatt
    10141 ttgaaggagt gaagcaattg attattattc cggcgattga tatcaaagat gggaaatgtg
    10201 tgaggctggc gcagggcaaa tttgatcgtg tgacaacgta tgcggacaat cccctggata
    10261 tggcgctgct ctgggcccga aagggtgcgg aattgatcca catcgttgat ctggacggtt
    10321 cggttgccgg aagaccgcgc aacgcggata tcattctgaa tatcgtggag aacatcaatg
    10381 ttcccgttca gatcggcggc ggcatccggg atatggagac gatccgattt tacctggatc
    10441 agggtgtctc cagtgtcatt ctcggcacaa ccgccctgaa gaatcaacag attgttcgcg
    10501 aagcctgtac ggtttttccc ggcagaatta ttttgggcat tgacgccctc aatggaaaag
    10561 ttgccgtccg gggctggacg gaaaacacgg agaaaaatgc ggttgacctc gcccggcagt
    10621 atgaacccta tggcgtcaag gccatcgttt acacggatat cgagcgcgac ggcatgggaa
    10681 ccggaatcaa catgaaagcg acaagagaat tggccgattc cgtcagcatt ccggtgattg
    10741 cctcaggagg cgtggcatcg attgccgatg tggacaacct gatggtcgtc gaaggcggca
    10801 gcattttcgg agtgattatc ggtcgcgcgc tgtataccgg cgccgtcgac ctggaagagg
    10861 caataagcaa aacaaaaaaa gtgacccgca cgtaacaaga ggaggtggcg ttcatgtcgg
    10921 attgtgtttt ctgtaaaatt gtccggggag aaatcccctg tgccagggtt tttgaagacg
    10981 agcatgtact ggcgtttgat gatattcatc cgatggcccc ggtgcatgtt gtaatcattc
    11041 ccaaaaagca tatcgcaacg ttgatggatg tcgatgttga taactcggat gtgatccatc
    11101 gactgacggc tgccgcgcag aaagtcgcgc gaattaaaaa aatcgaccaa agcggtttcc
    11161 gcacggcgat taactgcaat gccgagggcg gacaggtggt ttttcatttg cacgttcatg
    11221 tgctcggcgg cagaaaattg caggacgaat tgggataacg aatggaaggt gaaaaaatgg
    11281 atatcaatgc gaaattaagt gaagaaatgg ttgccgctgc caaagccaaa gacaagatca
    11341 ggctttccgc ggcgcggatg ctgaaaacgg ctctgcacaa caaggaaatc gatctgatgc
    11401 gtccgctcca tgaaagcgag atgctgcagg ttctctcctc gatgatcaag cagcgaaaag
    11461 attccatcga acagtttgcc aaaggaggcc ggactgattt ggtggaaaaa gaggaagcag
    11521 aactgaagtt tttacaggaa ttcatgcctg cccagatgtc cgatgaagaa gtcgatgcgt
    11581 tgatcaaaaa aacgatagag gatgccggcg ccgtttccgt gaaggatatg ggcaaggtga
    11641 tgaaaatcct gatgcccaaa ctgaccggtg tggctgacgg caaggcggtt ggtgaaaaag
    11701 tgaaggcttt tttatcccaa taacatctcc gttcttcagg ctgataaaaa gcggacaaac
    11761 aggatgaatc gtgtacgttg atcaaagcaa gattgaagaa atcaaaaaca gattggatat
    11821 cgtcgatctt gtttccgaat atctcacctt gaaaaaggca ggccggaatt ttatcggcct
    11881 ttgccctttt catcaggaga agacgccctc ctttacggtg aaccgcgata agcagatctt
    11941 ctactgtttc ggttgcggcg aagggggcaa tgtcattacg ttcctgatga aaattgccga
    12001 caagactttt ccggaagcta tcagagacct ggcggtgaaa acgggcgttg ttctgcctcc
    12061 ggtttcctcc ggaaaggaag ccagacaaaa ggacttgctc aaggaaaata tcgtcagctt
    12121 aaattcgaaa gcggcgcagc aattttcgcg caatcttttt tctgcttccg gtactccggc
    12181 ccgccaatat cttcagcagc ggggtattgc agaggatgtc gtcaaacagt tccgtctcgg
    12241 atacgcgccg gacacatggc gctctctgac cgattttatg gaaaaaagcg gcctttcgct
    12301 gaaactggcc gaacaggccg gcctgatcat acccggtaag gaagggggtt tttatgaccg
    12361 gtttcggggg cggctgatct ttcccataga aaacgttttc ggtgagatcg tggctttcgg
    12421 cgggagaatt ttggggaagg gcgaacccaa atatctgaat tcaccggaat cgccggttta
    12481 catcaagggc aaaaatcttt acggattgaa taaagcgaaa gacgacatac gaaagaagga
    12541 ttttgcgctg attgtcgagg gatatttcga tttgatttca ttgtggaatt ccggaatccg
    12601 taatgttgtc gccactctgg gtacggcgct gaccagggat catctggatc ttctgcgacg
    12661 ctattgcgtg gacgtggttg ccctgtttga tccggatgaa gcggggaaaa aagcgcttga
    12721 ccgcagtctg gaattatttt taagtacgga aatgagggcc cgggctttaa tcttgcccga
    12781 cggatatgat cctgatgatt atgtccggaa attcggccag gataagatgg aagatctgat
    12841 tgctcttgcc cagccgctca gcgattatta tattgaaaat gtcatcgggg gcggcaggac
    12901 ctttgaagat aaaagagata tggtcaagac ggcgatgacg tttctcagca aaatcggcga
    12961 tcaaaaagaa aagaacctgt ttatcaaacg tatagcggaa aaaacgggtt tgaatgaaga
    13021 attgctgatc agagaagcct acagccgtgt ttattcgggg aggaccgaaa caaaccggaa
    13081 taacccggct gtgaatgttc atcacgatac ggtggagttg catttgattc aactgatgat
    13141 ggaatatcct cagaaaatcg ttcaggtgga aaacgagaat gtgctggatt attttctaca
    13201 gcccgatatc cgaaaactgg ggaaaaaaat catcgatgcc tacaaactcc tcggcttcgt
    13261 ggatgtcggt gtcattttat cgaccgaaga aaatcaggca ttgcgggaaa gaatatttca
    13321 gcttatgatc aaagccttgc cggccgagga tgatgtgctg ggaaaaatat tgaccgataa
    13381 catccggcag attaaaaaac ggtggtttaa ggagcagcat cgtcagatca aaataaaatt
    13441 aagccgggcg caggatagcg gcaacgaaga gatggttcat cagctgcttt atgaaaaaga
    13501 gcaaatgctc ataaaggaaa aggaattgca atcaacaggt tgaatgaacg acgggaggat
    13561 ggctttcgcg acgcggaagg gttcaacgga tttgttccgt cgtgtttatg gaaccggggt
    13621 taaaaaaaac atttgaagga gatgccagat ggcaaaagaa gttcagtccg acgaagtcaa
    13681 taagttaatt tcactgggag aagaaaaagg ttttttaact tacgatgatg tcaatgacgc
    13741 cctgttgccc gatgtgacat ccgcggaaca gctggatgat attattacac tcttcggtga
    13801 aaagaacatc gatatcattg acacggaaca gggcgaaaag ctgaccctga agaagtcgat
    13861 gaaggaaatt accgacgtga atcttaagga agccctggcc attttaccca gtgctgaaaa
    13921 aacgggcgat cccgtgaaga tgtacctgcg cgaaatgggg tcggtatccc ttctaagccg
    13981 tgaaggggaa gtggaaattg ccaaaaggat cgaagagggc gcgcgcgaaa ccatggcggc
    14041 gatcttccgc ctgtcgattt ccatcgatga ggtgctgact atcggagaga aactgatatc
    14101 cggcgaactc aaaagcaaga atgttgtcga caacgtcgaa gatgaagaag gcttcattga
    14161 ggaagatgag cataggaacc gtattttgaa gcaaatcaat aaaatccggg aacattacga
    14221 tattatcaaa gcgacgcgtg ttaaactcaa atcaaaaaat cttacccaaa agaaaaaaga
    14281 tacactgcat gccgaattga aaagacgcat agaattcatt gtcaggctct gtcgcaagat
    14341 ccgttttagc aagaaacaaa tccagcgttt tattcaacgc ttacggaatt atgcagacga
    14401 gattgatgct gcggagaaga ttatttcacc gtacaggaaa gaaacaaaaa catccgcccg
    14461 ctcgaaaaaa gataaaaaag ggacctcgcg gaaggaagcg gttcaggcaa aggcacccgg
    14521 aaaaaatgac gcggcagtca aggaagcgca gaagaagata aaacaaatcg aaaaagaagt
    14581 cggcatatcg gcaacgacgc ttaaaacaat catcaggcag attgaagacg gcgaaagaaa
    14641 agcggagaga gcccagcgga cgctggtgga ggccaatctg cgtctggtcg tgagcattgc
    14701 caaaaaatac accaaccgcg gattgcagtt tcttgacctg attcaggaag gcaatatcgg
    14761 gctgatgaaa gcggtggata aattcgagta tcagcgcggc tacaagtttt ccacttacgc
    14821 tacctggtgg atcaggcagg cgatcacccg tgccattgcc gatcaggccc ggacaatcag
    14881 aattcccgtc catatgattg aaacgatcaa taaactgata cgcacatccc gttatctggt
    14941 gcaggaactg ggacgcgaac ccactccgga ggaaattgcc aataagatgg agtatccgct
    15001 ggagaaggtg agaaaggttt taaaaatcgc caaggaaccc atttctctgg aaacgcccat
    15061 cggcgaggag gaagacagtc atcttggtga ttttattgag gataaaagag tcatgtctcc
    15121 ttcggaagcg acgattaaca tcgatctggc cgagcagaca cggaaaattc tgacaacatt
    15181 gacatcccgc gaggaaaaag ttttacgcat gcgcttcgga atcagcgatc gatcaaatat
    15241 cctcacccgg gaggatatga ttgtgacaga tactcttgaa aagatgccgg aacttccgga
    15301 aacgcctgag atgcccgaag ttgcagatgc tttgtcgatg agcggcacga agaataaaac
    15361 gcgaaaacgt tacacaaaat aataaaatgt tattgacaat atgagtattt gtgtatatca
    15421 actcactctt tagaagatac tcggaaaagg agcgggccta tagctcaact ggtagagcca
    15481 ccggctcata accggtaggt ccctggttcg actccaggtg ggcccaccag acccaacaag
    15541 acagatgacg gaagaaaaaa aaatatatca ggaagttcag gagatttcca aaataagctt
    15601 cctgactgcc gtaatgagac gcacggagtc atcattatac ggcacagaca agaaacccgt
    15661 gaagcagtct gctcaaaaaa tgcaccccta aaaaggtgct ttttttttaa tgacactcgc
    15721 tgagagcgaa cgaagtgaag ctcgaagcgc aagtggaatt atccccgcag tgaggcgaat
    15781 ggggattgac acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagctcga
    15901 agcgcaagtg gaattatccc cgcagtgagg cgaatgggga ttgacactcg ctgagagcga
    15961 acgaagtgaa gctcgaagcg caagtggaat tatccccgca gtgaggcgaa tggggattga
    16021 cacaagctga gagcgaacga agtgaagctc gaagcgcaag cggtagtgag gcaagctgaa
    16081 cgatagtgaa gcgtaaaccg aactatccaa tcccggtgta tcggggttgg atactattcc
    16141 tgcagcgaag cggaatggat tcttgttaag agcaagacga aataaaaaaa atgaccctga
    16201 acttcatgtc agggattgaa aaaaagaagg agaagatgat tgaaagagca attattatta
    16261 ctgataaaaa ttcaggaatg tgattccgaa ctggtgaagt tgtccgcaaa gaaaaaaaca
    16321 ctgcccgaga agatcgagac actcaataaa gaatttaatg catttgaaga tggcataaaa
    16381 accagcaaag ttaagtatga tgaactcaag ttgcgtcata cggatagcga aaataaaatc
    16441 aagaaaatca acgaaagcat agtcaaaacc aaagaaagaa tgctggaagt taaaaataat
    16501 aaggaatatc aggcgatgct caaagaaatt gagattgcag aaaaatcgcg tggcgaaatt
    16561 gaaacctcga tcatttccat actggatgaa ttggataaac tttctgtgct ggtcaaaaaa
    16621 gatgacgacg tattgaaaga gagaagacat caatacgaac aggaaaagaa aacgatcgaa
    16681 gatgaattga atgccgtgga caccgatgcg gcgaactggg aacaaaaaag aggggattta
    16741 cagaaaaatg ttcctgaaga tctgctggtc cgatacgaaa gaatcaaaaa acgaaacaga
    16801 ggcgttggcg tgatatcggt ctggaaatcg gtctgcaacg ggtgccacat gaacatcccc
    16861 cctcaactct ataacgaagt ccagaagtcg gaggagttgt tcagttgccc gaactgcaat
    16921 cggattatct attttaagga tatggtcaag ccatcataag ggatatgtct gaaaaatatt
    16981 acaaactgtt cagcgacggc gcctgccggg ggaatccggg catcgggggc gcgggggctg
    17041 taatcaccga tgatgctgat catgtgattt gggaaggaaa ggaatatctc ggtcactgca
    17101 ccaataatat tgccgaatac aaagcgctta ttctgggact caaaggtgcc ctgtctcagg
    17161 gatatagaaa ccttcaaata tttttggatt cggagcttct ggccaaacaa atcaacggcg
    17221 cctaccgtgt caagaatgaa aatctacaga cactcatgaa agaagtccgt catcttctgg
    17281 tgtcttttga tcgcgttgag gtgaaacatg tcgcgcgtct gtacaacagc catgcagaca
    17341 aactggccaa cctggccatt gatgaaaaaa attagttttt agggattgaa acgaaagatc
    17401 attaccgaac agttatccgt tttttttctt catccagggc tttttttacc tgcgattcat
    17461 gcgccgaggt ttcttctctg atgttattga tcagcaggac cgcaccggga tcatttgaac
    17521 tgaaataatt ataaaatttg tgttcgatga tggattgctc caggctgagg ctgaacgata
    17581 aagcgttgac gacggagtat tgatggttct ctgtcttttt atatttatcg cggatatcat
    17641 cgataatcga tcgaatcgtg taggttttgg tcattttttc atcaaaggtc acatggccgc
    17701 tcaacgcgaa tgcttttaaa gtgcggacgt attcggcgtg ttgcaattct tcccggctca
    17761 tctcagccca gagatcctga tgtccgggaa attgctcggc gaacgattta tagagattgg
    17821 agatttccat ttccaattgc tcgagaaaac ccagcaactg catttgataa tctttcagca
    17881 tgtgacgtta cttttccgcg ttattttttg taatgttgtc cggtgacggc catattgtcg
    17941 cgatgaatga tttcatcata atccttgtgt ccgagaacct gaccgatttg ttttgttttt
    18001 aatcctttga ttttattaat ttctatggaa ttataattca ccagtccttt cgccaggatc
    18061 gcaccattgt tgtccacgca gatgaccggg tctccgtggt caaagcgccc ctcaatgtca
    18121 atgattcctg aaggaagaag gctcttgccc tcttccagca gtgctttttt cgccccgtcg
    18181 tcgatcacga gtttgccgcg gggacgcagg gtgaaagcga tccagtactt cttgctgttc
    18241 aaacgatccg tcatgggtaa aaataaggtt ccgatttcgc ggcccgtcat aatatccgtc
    18301 aggatatttt tcttttttcc gggcgcgatg atgcagggaa tgccgatagc cgtcactttc
    18361 ttggcggctt gaaccttgct tttcattcct cccgttcccg aggaggatgt ttcttcagtc
    18421 gccgcggctt caatttcttc agaaaattca gagacgacac tgatcaattt cgatttcttg
    18481 gatgaggccg gattgcaggt gtagaggccg tcaatgctgg tcaggttaat aaagagatcg
    18541 gcttcgataa tattagcaat catcgcggcg agattgtcat tgtcgccgaa ttttatttca
    18601 tccactgcaa ccgtgtcatt ttcattgata atgggaatcg ttccccacgc catcagtgtg
    18661 gaaagggtgt tgcggatatt caggtatcgc tggcgatcgg tcaaatcgga aagcgtcaaa
    18721 agaatctgcg ccacataaat accgtttttc tcgaaggctt tggaatagac gcgcatcaat
    18781 ctgccctgac cgatggctgc ggcagcctgt ttttcaggaa tacttttcag attttccgtg
    18841 atcttcaacc ggtgttttcc cgagacaatc gctccggagg ttaccagcac gaccgcataa
    18901 cctttcttgg cgagattcgc catttccagc accagagaat cgatgatttt cagatcgaga
    18961 ccgtcatctc ccgtcagtac ggccgaaccg actttgatca gaattttttt tgcgcgcgtg
    19021 atgatatttt gccgaatatt ttccatgaca tcaactctaa ccggtttatt ttaacttttc
    19081 agcaatcctg tttaatattt cctttacccc ttcgccggtg actgctgaaa aagcgtgtaa
    19141 tattatgccc ttttttttga atagagcaac ttcttttttg attttttccc ggacaaacgg
    19201 aagatcaatt ttgttgatcg ctgcgatttg ctctttttcg gccagagccg ggttatattt
    19261 tttcagctcg tcgttaatcg ttgagaaatt ggaccaggcg tcggtatggg gggtcatgga
    19321 gacatcgata atatgcaaca gcaatgacgt tctttcgacg tgtttcagaa atttatcgcc
    19381 catgcccatg ccctcatgag cgcccggaat cagtccggga atgtcggcaa tgacaaaatt
    19441 tttatcgtct gcatattgca cgacgcccag atgcggcgta agcgtggtga aggggtaatc
    19501 ggcaatcttg ggtttggctg cggaaacacg ggagataaag gtcgatttgc ccgcattggg
    19561 gaaaccgata atgcccacat cggccagaag tttcagttcg agtttgagcg tgaattcatc
    19621 gccttccatt ccttcctggg cgaagcgggg cgtctgatgc gttgaggtgg caaagtgaga
    19681 attgcccttg ccgccgatgc cgccacgtgc ggcaatatac gtctgtcccg cttttgacag
    19741 atcggcaagc tcttctccgg tatcaaaatt tttgatgatt gttcccaccg gaacggagat
    19801 ttccagatcc ggagcacttt tcccggtgcg gtgattgccg gaaccgtggc cgccgttctt
    19861 ggcgatttga tgctgcttgt attttaagtc caacagcgtg tgatgacttt ccgttgtgcg
    19921 aaagatgaca tcgccgcctt tgccgccgtc tcctccgtcc ggaccacctt tcgggacaaa
    19981 tttttccctc cggaaactca cgcaaccgct tccgccgtgt ccggccttga cataaatttt
    20041 tgcttcatcg acgaatttca taaccttgtt cagccgtatg ttcttttgag ttaagataaa
    20101 ctatatttaa tacgttgatc ccttattccg catacgcggg ataatccggc tagccaattc
    20161 caatgcactt cgtttttgga attggagcct cattaaaaac aaaaggcgct tgaagtaagc
    20221 gccttaaatg gttgtaatcg atccgatcga atattcttat gctgcgtaaa cgcttacttt
    20281 cttcctcgtc ttatcaagtc tttcatattt tacaacgccg ttaatcaggg aaaaaagagt
    20341 gaaatctttg cccattccca cgttgttgcc cgggtgaatt tttgttccta cctgtcgcac
    20401 aatgatcgag ccggcgtgaa tttgttctcc gccgaatacc ttaattccgc gtctctggga
    20461 attgctgtct ctgccattgc gggaacttcc ctgacctttt ttatgtgcca tgacttcctc
    20521 cgcttatatc gaaattttct taattttcat gctggttaga ttttgacgat gaccggtttt
    20581 tttatggaaa cctttccgtc ttttcatatg gtaaacagta agtttcggtc ctttcttctg
    20641 ggcgataatt tcaccgacaa ctttggcgcc gtccacaaaa ggtttgccga cttttatctc
    20701 tttatcgtcg gagaccatca gcacatcgct gaaaacgact tcatcgcctt tattcccgtc
    20761 cagtttctcg acggataaaa tgtcgccttc gcttaccttg tgctgttttg ctcctgtttt
    20821 aatcactgcg tacataacgt atccttatga aaattgaatt aagactgatc tttatagaca
    20881 aaacgtcccc gtttgtcaat aaattttaat ccggacagga gaaatatttc tatttctgcg
    20941 aaaaatggat aagttgctga tatcatatga aaagtaaaaa aatagaaaca ttttatgccg
    21001 tgatacggtg cactggcaag aaacaatttc accatgtcgg ggcaaaaacg aatttgaaaa
    21061 atactgttta tttttctgcg cagttatgaa ataagaaaaa aatacgaaca caaccatata
    21121 ctgtaagaac catgtgccgt taaaatcata cagttcaacc aaggaggttt atcatgaatg
    21181 ctggcgcacc agtggcgctc atcagcggat gttcaacggg aatcggacgt ttcctgacca
    21241 cggaactggc gaaacgcggc tggcaggtgt ttgccactgc ccgcgaccat gcggccattg
    21301 aggatctgaa agcgccaaat gtgcaaaccg ccgccctcga cgtaacggat gaaaaatcga
    21361 ttaaaacctg cgtggaatcg gtcatggcca acgccggtaa aattgacatg ctgattaata
    21421 acgccggtct tctgctcatc ggccccttgt cggaactgga aatcagcgag ttgcgcaggg
    21481 agtttgaaac aaacgtcatc ggactggcgg ctttgaccca tgcggttgtg ccgcacatga
    21541 tcaaaagaac atcgggaaaa attgtcaata tcagcagcat ctccggtgtt ctgaatacgc
    21601 ctttttcggg cgcgtactgt tccaccaaag cggcggtgac ctcctattcc gatgtgttgc
    21661 gcatggaact gaaacctttc gggatcgacg tgattaccgt tcaaccgggc ggcatcaggt
    21721 caaatttatc ggataacgcc gacaaagggc ttgaacgttt ccgaaaaacg ccctatgccc
    21781 cgataaccga ttttattgtc gatcgtgcgc acatgtcgca acagggtgcg acacccacgg
    21841 acgtcttcgc gagacaactt gtcgacaaac tcgtgaaaag taaaacaccg agaatcatcc
    21901 gcatcggaaa gggggcgacg atgtatccct ttgtcgccaa attgccccgt acgtttacgg
    21961 acagactgct ttcccggaag ttcgggctgg acaaacttta ggaaattcgt caattaagaa
    22021 aatagcgttt gttggtgcgc gattattttc ttgacataaa aaatcacggc acttatattt
    22081 gctccatcaa aacttgctcc tcgttcgaaa atacaatgtg gcagtgagat gatttgacat
    22141 gaaatacggc attatcgcga accctgtttc cggcaaacgc tctgtagatg acaaaatcaa
    22201 aattttaaaa gaagtagccg gtattttaaa agacgccacc attgccggtc tcgatacaaa
    22261 atctcccgaa gaattcagca ctgcagcgca tgacctttcc caaaaagtgg ccaggctgct
    22321 tgtcgccggt ggggacggaa ccttttcact ggtgatgaac agcgtttcca gagacatccc
    22381 tctgggattt ctgccgttcg gttccggcga tgtcctgcgc cataccttca accttccccg
    22441 ttgtcccaaa gaaaatgctc tgttgattca gaaaggcaaa gaatatttgc tggatgtcat
    22501 ttgctgcgac gggaaaacaa aagccttgtt cgcgagtatc ggcattgaag gaagtattct
    22561 gttagaacgc gataactatc tggaaaataa tttgaccggt tttaccgcct atggtctgtc
    22621 gacattaaaa attttatttg acggatacat caaggctgat ttgcgtgttt tgatggacaa
    22681 aaaggaaata agaaccttca actctctgac cgccgttatt acaaaacttc cctactacgg
    22741 ctacggattc aaggttgtcc ctcatgctca atttgatgac ggatatttgc atttgctgat
    22801 cctgaatgcc ggacccatcg gcacgctcta tgctttggca acttctcttg cgggggggaa
    22861 cagaatggga gagtattaca aagcgaaaga aattgatatc accacttcaa cggagcaatt
    22921 gctgcagcgc aacggcgatc cggaaaaaga aaagcgaaac aatttttcct tcaggatttt
    22981 acccaaagaa cttaaactga ttttttagtg aactgattcc cccgttcagc caagcctttt
    23041 tgacctggcc gcaaataaaa ataatccgta gagtccaaaa aactcggctg atcttaatgc
    23101 tatttggaat ggagaaactc catgaggcaa tttaattcag acaggcgtct gatcgtgaaa
    23161 acagcgcaaa agcttgttga aaagggttat cttgcggcaa ccggcggcaa tatttccgtg
    23221 cggattaaaa aaggaaatgc ttttgccata acaccgtcca atcgcaatta catgaccatg
    23281 accgcggccg atatttgtat tcttgatgaa cacctcaacg tgctttcggg gccatggaaa
    23341 ccatccatgg aagcggcttt gcacggtgcc gtttatagaa cacgcggcga tgtcaacgcc
    23401 gttatccaca cacatcaggt ttatgcgggc gcgctgacaa ttatcaaaac gccgctgccg
    23461 gcgctgtttg atgagcaggt gcgttttctg ggcaaaacgg tggcggtcat tccttatgct
    23521 ccttcgggat ccgtgcctct gaaaaacaat gttctccgac acatcaaaaa cggcaacaat
    23581 gccttcctga ttcagaatca cggcgcgctg gttttaggtc acgacatgga tcgcgccgtt
    23641 cataatgttg aaattctgga aagatattct ctggcgtatc ttctggcgat ttgcagcggg
    23701 tacagggtga gcaaaattcc catgaggatg cgggagacgt ttttcaagat gctgcgtcaa
    23761 gagcaaaaga aactgcagaa agaccattgc ggttcgagga aaaaataaaa gatacggatg
    23821 attgtttcat ccaatctgcc acgccggcaa ttcacccagc gccgttgatt ctttcaggta
    23881 gacaatatca tgcaaaacgg ccgtgcgctg tatctgcttc aaaagatgtc gtgaagattc
    23941 ttttttcatg gcggttgcgg gatgcggcgc gtacgcgcaa acgaaattcc acatctgttc
    24001 aagagcgttt ctgagattgg cttcgacggg cgggcagcga agcagcgaaa ccagttccgg
    24061 ataaatctca tggattccct tgccgctttt ccgggatgtc acccaaccgc cgatgtattt
    24121 gtattcagct tcatcgcgcg ccatgaccga atattgatgc tgcgcccata gctgctgaac
    24181 actattgggc aacggaatgc gtccctgttc tttgttgacg tatttctttt tcaggatgga
    24241 gaattgagac gtcggagaat cgacaaatac atgcggccat ttttgcggtt gcgttttgat
    24301 catcgccggc gacctgtccg tgaaaccgcg cagattcatt tccgccgcca gtaatctgtg
    24361 gcgctggctc aatgcccagc cgaaaccatg ccagcgcagc gtttccggat gccgcgaata
    24421 tcctttttta ttgtgctgga tgatggaaac aatcgcgtgc agctcgcggt gttcacccaa
    24481 taagctttgc cggtttaaat aaccgggatt gacgtcccaa attctcacaa gctctcctta
    24541 ttgataaagc tatccgagca gtgttttggt tttgaccacc ctgatttgac gcgattcgtc
    24601 ggggtcggat ttcaggatgt gggcgatgat atcgacaggc ctggccgaac cttcctgaac
    24661 aaaggatacg ataaatttta ttattttctt gtcacggtct agggaaagca tttgcacaaa
    24721 aggacgaatg tctttggtta tggttttccc tttggatatt ttcttgatgt tgaaattgga
    24781 tgccgccaga aattgatcga tgtttgcttt tatggcgctc attcggggag cgtcggtagc
    24841 ggcaggcagt tgcagttcat attcgaatcc ccttgtcgct tgagcgatag cattttcttc
    24901 cggagatagc tttctgattt ccaaaatttt aatacctgtg ggaagcagag tgttgatttc
    24961 agttcttaat aaatccagac tgcatgaata ttcctgtgcg ttgatgtcca tatattcctg
    25021 tctgctttcc atgcctacag cggttgccgt ggcaaaggaa atcttgggat gcggatgaaa
    25081 accaccggta taagcaaggt tgatgctgct gcggcgcaag gcgcgaacca gagcggtggc
    25141 tagttccaga tgagacagga atccggcgct ttccatttta ttaaacataa cccgatattt
    25201 ttcttcttgt gccgaagaag tctcgggtgc caaagacgat ggcaacacag atttgttttc
    25261 gttgtctgcg gcgagaatat ttttcgtgat cgaaaaatcg catgctccgc aattccggca
    25321 agtctcaaac cggcaatctg ctgttgccgt ctgctgaaac gctttctgct tttcctccaa
    25381 aaggaattca cggctcacgc cgcagtcaat gttgccccat ggcaattttt cgtcagtccc
    25441 gcgttcgcga aggtagtcgt cgggatgaat gtccgactcg gcgatggctt cttcccagag
    25501 gttgaaacgc agaatatcag accaaccgtc gaagcggcaa cctttgcgaa acgcatttcc
    25561 cagaagtttt cccgcttgct cattgccgcg cgaaaaaatg ccttccagaa aactcatccg
    25621 cgcgtcgtgc catttgacgc tgagattgcg gtttttgatc cgcgtgcgga tgaaatcctg
    25681 cctttcataa gtttctgcca gtgaaatctg tttttcccat tgaaagggag tatgcggttt
    25741 ggggacgaag gtggaaagac tgatggtaac ctgaccgcgg ttttttgccg cgcgcagggt
    25801 ttcatggctg agattgacga tgccttccca gtcctcctgt gtttcatgag gcaggcccag
    25861 catgaaatag agcttgatgg atttccagcc ggcggcaaat attttatccg cggtggaaag
    25921 taaatcttcg gaggtgttgc ccttgttgat aaccagacgc attttgtccg ttcccgcttc
    25981 gggcgcgagg gtgaaactgg tcttgcgcgt gcgcctgatt tcttccatta aagtcgtgtt
    26041 gagggattcc acacgcaggg aagggagggc caacgccacc cgcttttgac aataccggtt
    26101 catcaggccc tttaccaaag gttctagaca actgtagtcg ccggagctga gggataaaag
    26161 cgatatttct tcatggccgg ttgccgcgag catcttttca gccatttcca taagcagggc
    26221 ggcgtttctt tcgcgataag gccgccagat cattcccgcc tggcagaacc ggcagccgcg
    26281 ggtgcaaccg cgcgcgattt ccagcgtgat gcggtcgtga atggtttgca tcagcggaac
    26341 aacgggtttg aggggatgcc tccaggcatt gagatcgctg acggttcgtt ttttgatgat
    26401 ttcattgtca tgatgaacgg cgggaacata catgccgggt atgttcgcca gttcgccaag
    26461 cagcccgttt cgggagatgg attttatttt gccttcgcgg acggctgcaa caatatcgcc
    26521 gacgatttct tcgccttcgc cgataacgaa cgcatcgata aaatcagcaa gcggcgcggg
    26581 attaaaacag cagggaccgc ctgcaataat cagcggatgt ccgtcccggc gttctttacg
    26641 gtcaatcggg atacctccca ggtccagcat attgagcata ttggtatagg aaagttcgta
    26701 ttgcagtgaa aaaccgatca tgtcaaaatt ctgaagcggg atttgacgct ccaaagacgt
    26761 caacggcaat tttttgtcac gcagtatttt ttcccggtcc ggccagggcg caaaacaacg
    26821 ttctgctgac gcgtaaggga tgtcattgat aatcgaatag agaatctgca atccgagatg
    26881 cgacatgccg acttcgtacg tgtccggaaa ggccagcaga aagcgaacat cggctttatc
    26941 tttacggacg gcgtttactt ccgtgccgat gtaacggctg ggtttctcgg cggaagataa
    27001 aagctcatcg attttgccgt ggcgcataat gattttttcc tttttgggca aaggtcccgg
    27061 atctatgctt ttttgtcgga ttgagcgtgg gatctgtatt tatgcggatt gctttttatt
    27121 tccctgagct tggataatgc cattttttga gcaaaattcg cggcggagag atatcctaaa
    27181 gaattatgct taaatgtgct gattaactca ttgcgggtcg aatcttcatt gtctttggac
    27241 acgaacgttc tttccatggt gtgttcgtaa gccagttcgt cgcccataat attttttacc
    27301 agacgataat cttccgtgct ggtgaaaaaa gattctttca gtaaaacgtt tgtttggaag
    27361 gaaacttcca ccttgaccgt atctgtcgct atggaacggg acaaatcgaa aattcttaaa
    27421 tccagcccgt tatctaattt tatttcttca atcagtttca ttttgctcat ctcaatccat
    27481 ttggtttaca agacggttat taacattttt atacttgacc tgcaacttta atccttttat
    27541 acgtagctcg cagaggaaac tggtgaatgt tttatgaata atgtcaatgc tgttttgctt
    27601 ttgtcctgcg ccgacagcaa gggactagtg gccagaatat ccgattttat tttccgtttc
    27661 gacggcaata ttgtgcacgc cgatcaatat acggcaaagg cggaaaagct tttcttcatg
    27721 cgcgttgaat gggagctcaa cgggtttgct ttggatcgtg acgacattcc cgtagcgttc
    27781 gcgccgctgg ccaaacaata tcagatgaaa tgggatttgc attttaccga ttacgtcccg
    27841 cgcgcggcga tttttgtttc caggcatctg cattgcctgc acgatctgat tcttcgtcgg
    27901 gacatgggag agctggcggc cgatatcaaa gcggtcatca gtaaccatga ggatgcccgc
    27961 gatctggcgg aacagtacgg gatgcagtat ttttattttc ccgtaacggg aaaaaataaa
    28021 aagcagcagg aaaagaaaga gctgaggctg ctgcgggatc tccatatcga tctcgtggtg
    28081 ctggcgcgct atatgcagat tctcagcggc aactttctca aatattatcc cagcaggatc
    28141 attaacattc accactcgtt tctgccagcc tttgccggca ggaatcctta ccagcaggcc
    28201 catgaccgcg gtgtcaaaat tatcggcgca accggtcatt atgtgacggc gcagctggat
    28261 gaggggccga ttattgcgca ggatgtcgtg aaaatcagcc atcgtgacac ggtgactgat
    28321 attcagagaa aaagcaagga tctggaaaga attgtcttgg cgagggcttt acgcctgcat
    28381 ctggaaaaca gaatactcgt ttacggatcg aagactatcg tgttcgagta aggaagtatt
    28441 gtaatgttgt ttgaagacgt cacaccggtg cggaccggtg tccggaaaat gctgcaaaaa
    28501 agagactccg gccttcgccg aaattatgaa agagagcaat caacaagtat tttacaggtt
    28561 catcagatct gataatcatt atacagaccg gataattatt tatgtcgaat tccaatattt
    28621 tcgtcctttg tcagccggat aagggaaaat cctgcggcgc gtgttgcgga ctttataatt
    28681 acgtggacag ctcccgttct tctcttgcac ggcgtctgcg ttcgcgcacc aaacgtttcc
    28741 gtgaaacagt aaaaagtcca gccgatctta agcgctatgc gactgaaacg ctggtcgcgg
    28801 aagatttcaa aaagagatat gacgtgattc actgctgcga atatctgggg tttctcgatc
    28861 acgatgaaaa gaaagtcggc tgtcttgtgc atccggaaca aaacgcaggg tgcgatatgc
    28921 gcgatgtttc gttttacggt cgcgaactgt gcgcggggca tctctgtccc agtcattatt
    28981 ttattccgca gtcccagtct caaattctga tcaaaatcat tgacgactgg tatctgtatg
    29041 gtctggtgct gacggatatt gatctggttg ttcagtattt tcgtctcatt gccgacaggg
    29101 tcggcgccga actcaaaccg gacgttttcg atcatgacga gttaaaagaa attgccctgg
    29161 attattttgg ctggaaactg aactggccgt ttcggtcgca ggaagcgaat cgtctgggca
    29221 aatattactt tgacggctcg caatacatga tcagtcatat cgagtacgaa aaattcggca
    29281 gagaggtttc tgtgctgaat tcgattttat tgagtctatg ttcttctttt aaaagtgcgg
    29341 tccaactgga ggaagcggag aagatgatct gggccaatat tgaaaaattt gtatccacat
    29401 ataagcggca tgtttcatga ggtttgctga gtacaaataa cagacggtat gatgcgttgc
    29461 ccgaattttt cacgccgaga aatatgcaaa gccgcactcc ggaatttcgt ctgatgctca
    29521 gaggataatt tttattgaca taacacaacg actttggata atcttaatac ccttttgggt
    29581 aatttataag caatttaagg ggatgtgcaa tggatcaggc gtccagaaag attctttttc
    29641 ccaaagaaac cattcaaaaa cgggtacggg aaatggcgaa tcaaatatcc aaagattatg
    29701 caggaaaaga attgattttg atcggcgtac tgaagggcgc ttttattttc atggccgatt
    29761 tgattcggga aatcaacatt ccctgcaggg tggattttgc aaggctggcc agttacggcg
    29821 ccggatctga gagctccggc aaagtcgtcc tcacgaagga tattgaaaca tccatcaaag
    29881 acaaggatat tttgattgtc gaggatattc tggacaccgg actcactatg cagtattttg
    29941 tggagtggct gaaggaaaga aatcctaaat ctctcaaaac atgtgttttt ctggataaac
    30001 ggaagaggag aaaagtgcct ttggaggccg attatgtggg gtttaccatt gacgacggct
    30061 tcgtggtagg ttatggactc gattttaatg aaatgtaccg cttttctcct gatatttata
    30121 ctattcattc gaataaactg taagacataa tatcagagaa actttcagaa attcatcatg
    30181 caatttttga aagcaccgtg tcgcaagcag taaggacaga cgttatgatt attcaatgcc
    30241 ggcaatgccg gacaaagttt cgcttcgatg attcgcatat gcagggaagc ggtatctggc
    30301 tcagatgcag ccgttgcggc catgtgtatt ttcaggaaaa tccttttgcg aaaagaaccg
    30361 attccggtgc gcttccgctg accgaaccgg accttgaaac gaaaccggtc aacgaggaag
    30421 aaaaaacagc tccttccgaa acggtgtcag tgccgccaga agaagcgcct tccatcatcc
    30481 gcagagacga gcacgttctg aaatccatcg ataagatcat gacggccaga aagcgtgacc
    30541 gcgaagggat cgatctggac atcgccactt tccgggatga aaaagtcggc gaagcagacg
    30601 gacagacggg tgacatcgta caagaggaac aagaagtcaa tgttgaagag accgaccatc
    30661 agaagaaaac gctcccgaaa ggttccggca gaatctggaa aacggctctc tggtcggttc
    30721 tggtcatagg ggttataccg ctgttgatct atttcttcat tttaccgcaa tatggcgcac
    30781 agctggtcgg cagcggtgaa tatctcttca aaaagatcag cgaattaaga ggcgctccgg
    30841 aactgccgac atcaggggct tatatcaacc agatggtgaa gcttcagaat gtgcgttacc
    30901 gcagaatcaa caattacatt ctaggccaga tcggtgttct cgaaggcacg gcgctgaatc
    30961 gtgcggattt cccgctgtcc cgggttcagg tgaaaggtga aatcgtggat gcctactcgg
    31021 tcgttttggg tgaacgttcc agttacgcgg ggaatatcct gaccgatgaa gaactgatta
    31081 atttgtctga agaagaaatt ttaatgaaac tgcttcgtcc ggaaggtctt aataacgcca
    31141 acgacaggat tatgcccaac ggacagatcc ctttcatgat tgttttcccc agcgaaccct
    31201 cgggtgtcat caagacaacg gtcatgacca tcggcgcgga acgtctcctt gagccctgat
    31261 cgggatattc attgaccgca gtattcaaaa aatcagcacg cattcgtccc cgattgaaag
    31321 gagtcaaaat tgagatacga aggaaatatt ttccggccgt tcagtgaagc caacagttat
    31381 cttctgcagt gcacggtcgg ctgctcgcat aatcaatgca ccttctgcgg tatgtacaag
    31441 gacaaaaagt acagggaacg gtcgcttgac gaaatcatgg aagacattgc catggccaaa
    31501 gaccattacg gcgatctgga aaaagttttt ctctgcgacg gcgacgcgat tgcgctgaaa
    31561 acagacatgc tgctgaccat tctgaaaaag ctgtacggga catttccgtc gctgcgccat
    31621 gtcggaagct atgtcggacc gcaaagtacg ctgtccaaat ccctggaaga attgcaaacc
    31681 ctccgggctg ccggtttgac caaggcatat atcggtgtgg aaacaggcga tgacgaactt
    31741 ttgaaaaaaa ttaaaaaagg cgtcggttat gaagaaatgc tccaggctgg tcggaacctg
    31801 atcgcggcgg gaatcaatct gtccgcgatg gtgcttctgg gcctggccgg gcgaggaaag
    31861 gcctctcgac ggcatgctct ggccacggca aaaatctgca atgaaatgaa accgcagtat
    31921 ctggccgcgc ttaccgtgac gcctgttccg ggcacggtgc tgtaccgtca ggtgcagcgg
    31981 ggcgagtttg aattgctgga cgcctttgaa accctggaag aaatgaagca gatattcgag
    32041 aatatcaccg ccgacaatat gaaatttgtc ggcacgcatg cctccaatta tcttccggtt
    32101 gtggggacgc tgcagaaaga caaacagaaa atgatcgcca tggtcgatga agttttgaaa
    32161 aacagagatg agcggatgtt gcgtccggat cacatgcgcg gactgtaaat gattttttct
    32221 gcaatgaagc agcaggtcgt ttagacgaaa attgtgttta tcggggacat ggatgtatga
    32281 tcatggtatc cggtgttcaa gattttcttg acacgaaagc agggtgtctt ttatgcttcg
    32341 gggcaatatc agggtaaaaa aatattttta aatggcggcg gacaatgatg gcaaatgtga
    32401 tggataaata tttcaatatg agaatcatct tatgcgtcgc gctgatgagc atggtgttat
    32461 tgttgcaagc ctgttcaatc aatcaggttt atttgtccga ttcagacgag gaggattaca
    32521 gcgcggaaga tataacggat caggagatga tgcgcgttgc aaaagtgaaa ttacagtcaa
    32581 cgggtatggt caagagagcc tttgatattg aagagcgcca cagaaaactg accctcaggg
    32641 aaaccagaca attgctcaag attttgagcc agcgatttaa gaaaagcggc ttgtccgtcg
    32701 gcgaatttga agatctggtg ctggagagga taggtcgcga ccttgaaatc attagcgcct
    32761 ttaatgagcg actgaaaaac gcggaggcga aagaaaaaga tcacgggtat gaattgtgca
    32821 aaaaaaatga attgctggaa gtgattaaag gttctgaaga cattgtggaa gaatatattg
    32881 aagacatggt caacggtatc ctgccggagg aatttgaaac gtcgctatct gaaatcacca
    32941 gacttgaacg gaccaaagag gacgacattg tgcccggatc attcaaaccc acatgcttta
    33001 aaggagccct gattaccatg ctggtcgctt tgagaggcga ccgcgccaat gtgtcggcat
    33061 taaagttatc cggcggaacg tggtcaaatt ccagcattat ctttcatact attccgatgc
    33121 cgggaaaaat cagcaggtca tcgattcatc cggattttga atcatttacc tattattata
    33181 aaggtgcttc cagcccctcg cagggggaac tggggttccc gcacagcggt tattatgcca
    33241 tcggctacgg cgccagaaaa tcctccaagg gcatagccaa tcctgaagat tgctcttcct
    33301 ggatatttca ccaagtattg caaacgcaat atccggtgac ctcgtatgac gtgatgcagt
    33361 ttttcagaat taaaaccggg acgggaatac gccacacagg ttacgccgcc actcctgaat
    33421 acggctggct taactcggtg ttcatccctg tagagaaaat aacgaaaaag aacgcgcggg
    33481 cgatgataaa acccggttat gtattgtgta tacggcgacc ggacgaagac ggtaaagtca
    33541 ttcgaaatca gaattacgga gtttccggtc atatggctat cgcgctattt gcgacagata
    33601 cccacatttt tgccgtggcc tgcacacgcg acgttccgct gatggaaggg atcggtattc
    33661 aaaagatcag cttttggaaa gagagaaaca gcgatatcat gattttgcga atgaataaat
    33721 aacgcgtttg atgaagctac gctccgattc aatgactgca tcacccctct gatggccgat
    33781 gccagtcgcc gatttggtta cgactcaccc aaaaaaaatc atatccgtta cgatgaaact
    33841 tcgatgtgaa ggggctttta cttgctgatc aaatgacgtg aagatgttct ttaatcgatt
    33901 gcctgctgat ggatccttca cgaaggacga ccggcggatt caacgtaaca tcaatcagag
    33961 tggatgatgc cccgcccggc gttttccccc cgtccagaat tgcgtcaatt ttgccgccga
    34021 tttgtccggc aacttctgaa gccgtcgagc attccggcgc gccggataaa ttggcgctgg
    34081 tggcggtcag gggttttttt atgttttgaa cgattttcag agcgattgga tcagacgaaa
    34141 cacgcagacc gatcttgccg gttcccgccg tcagcagcgg cgacagggtg tctgacgcct
    34201 gaaaaacaat ggtcagcgca cccggccaga acgcgtcgat cagttttttt gccgtttccg
    34261 gtacatccgt gaccagcaca ttgatatctt ccggttttgc gatgattaaa gagacgggat
    34321 ttttaaaatt ccggcctttg acggcgaaga ttttaccgat ggcttcttca tgggtggcat
    34381 caactcccag accgtaaaac gtttccgtcg gataggcaat gataccgccg tccgtcagga
    34441 tttttacggc gcgactgatc attgcgtctt cggaaatgtc cgttcccggc ttcaggattt
    34501 ccggcatgtg agattttcct gctttttctc cacgtcttta gccattttcc ggatgtaatc
    34561 gcggagtttc tttcccaatg tcttgttgtc aagcgcaagc atgcgaacgg caaacagtgc
    34621 ggcgtttttg gcaccggctt ttccgatagc catagtagcc acgggaatgc ctccgggcat
    34681 ctgcacggtg gaaagaagcg catcaagtcc gtttaaagcg gtagcgtcaa tcggaacgcc
    34741 gatgacaggc aggggcgtct gagaggcaat cacaccggcc agatgagccg ccgctccggc
    34801 gccgacaata atcaccttga tgccgcgccc ggctgcagct gaagcaaatt gtgttgtgcg
    34861 aagcggcgtg cgatgtgccg acgtcaaaat cagttcatga gcgatgccga attcttccag
    34921 tacctttgcg gcttcggtca taacgggaag atccgaatcg ctgcccatta ccacactgac
    34981 caaggcattc ctcttgacga tttttgcttt catgcaggct cctttattgg ctgatattat
    35041 tgctttttat tattttttat atctaacgca tcgggccttc atttgcaata gttattataa
    35101 aatcgatctt tttcccaaat ccagttgaca taacaatatg atcttcatat actcccgcca
    35161 aaatttctta cccgcactca aggagagatg taaaaaatga aaaataattt atatgaattt
    35221 ctggccggca acgattatcc gggacgcggt atttgtatcg ggatgacgcc cggcggcaaa
    35281 gccgtgattg cctacttcat catgggcaga agcaccaaca gccgtaaccg tgtatttgat
    35341 ccaattgaag ggggcatccg caccaaggct gccgacccgt ccaaaatgac cgatccgcat
    35401 ctgattattt acaatccggt actgatcttt gccaaaacaa ccatcgtgac caacggtgat
    35461 cagaccaata caatctatga tttcatgtcg caaaacaaat ttccgggctt tcatttcgaa
    35521 gccgcgttaa atacccgcac gtttgaagac gacagtccca actggacgcc gcgtatttcc
    35581 ggtatggtgg atatgaaaac aggcgcttac aagatcaata ttttaaaaag ttgcggcggg
    35641 gatgagaaaa gcgtgcagcg ttttaccttc aattatgctc agccgctgtc cggcgaggga
    35701 cactttatca gcacttataa atgcaacggc acgccgattc caagctttca gggcgaaccg
    35761 atgcgcgtca taatcgatga agaagatccc gaccggtatg ctgcaaaact ctgggatgca
    35821 ttaaatgaag aaaacaaagt cagtcttttt gtgcgcgccg tcgatctcaa gacgcaaacc
    35881 tgtaaagacg tcattattaa caaatataaa gcagtggagg aatgatcatg aacgaaatac
    35941 aactgaaata cggctgcaat cccaatcaga aaccggcgag aatcttcatg gcggacggca
    36001 gccatctgcc cgtggacgtc ttaaacggca agcccggcta tatcaacttt ttggacgcct
    36061 tcaacagctg gcagttggtc aaagaactca aggaaaatac cggcatggtg gcggcagcct
    36121 cctttaaaca tgtatctccc gcaggcgctg ctctgggtta tcctttggac agagtgttgc
    36181 gcaaaatgta tcacatcgat tccaaaataa agctctcccc gctggcctgt gcctacgcgc
    36241 gcgcccgcgg cgccgaccgc atgagcagtt tcggcgactg gattgcgctt tccgatgcgt
    36301 gcgacgtgtc cacggccaaa ataatcaaaa acgaagttac tgacggaatc atcgcgccgg
    36361 gttatgagcc ggaagctctg gaaatcttga aatccaaaaa aagcggcaat tacaacgtgg
    36421 tgaagatcaa tcccgattac gtgcccgttt ccctggaaca caagcaggtt tacggcatca
    36481 cgttcgagca gggacgaaac gacttgaaaa tcgacagccg catgctggaa aacattgtaa
    36541 ccgaaaacaa aacactgacc gacgcgcaga aacgcgatct cgtacttgcg ttgattacct
    36601 tgaaatacac acagtccaac agcgtttgct atgtacagga cggccaggtt atcggtgtcg
    36661 gcgcaggaca gcagagccgc attcactgca cgcggcttgc cggacagaaa gccgacaact
    36721 ggcaactgcg ccatatgccg aaagtgttga atctgccttt ccgcgacgat gttgccaaac
    36781 ccaaccgcga caacgccatt gatgtttatc tgggcgaaac accggaagat gtcatcggcg
    36841 atgatgtctg gggccaaaca tttacgaagc agcccaaatc cctgaccagg gtgcaaaagc
    36901 agaaatggct cagcaaggta accggcgttt gcctgggaag cgacgcgttc ttcccgtttg
    36961 gcgataatat cgaacgcgcc cgccgcagcg gcgtaaccgc cattgtcgaa ccgggggggt
    37021 cgatccgcga ccagcaggtc atcgacacct gcaacaaata caggatcgtg atggcgtttt
    37081 gcggcctgcg cctgttccac cattgatatt tctgtcatgc tggcgcagga acaggaataa
    37141 aaaaaattga ccataatcaa gacgggcgag tccaaaagac tcgcccgaat gcagacagca
    37201 aacagaaaga tcggaagtca aaaaacgttt acatactcat tattattcta agatgaaaac
    37261 ctgaaaaatt ttctgtacca cgggatttta tgccctccat ttttcaatat ggatgaataa
    37321 ctttttatta taaaagcgat attgtatttt tccagcagat gcttcagttc tgtctccggc
    37381 tttattttga atcttgaata atcgctgccg acgggtttgg tgtgtacggc ggtgatatcg
    37441 tcaataatgg ctattttatc tttcggggaa ttcaaccggt gagaccatgt tgcatccagg
    37501 ccccagccgg attgggaaag atcgaaatca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37621 nnnnnnnnnn nnggtgagac catgttgcat ccaggcccca gccggattgg gaaagatcga
    37681 aatcattacg gagaaacaag agagtgttaa catcgaacag cggcatcatg acttcgacaa
    37741 agtttgtaaa tcttaacgtg ttttctcttt ccggtttcgt tatggcatgc gatacatcgc
    37801 cgtgtaagga tactaacgcg gactggcaaa tctgtagaga gtatgtccgg gctgtatcaa
    37861 aaagacggtc cagatcaccc gctgatatgg aaatgtcatc atcgggaagc caaacatagt
    37921 catattgaga tacgagttcc aaattcgttt caataaagga tttgattaaa ggaaatttct
    37981 gaccatgttg tttgatgcat atcaaggcat cttttgtata gtcgaaaaaa atcaaatcac
    38041 tgttaccgta ataaatgagg atcaaatcaa aggcggggtt gccgcttttc cattgtttgt
    38101 gcagggagct gtctccgaca gcggctataa ccaaattttt gttcttcatc attcaatcgg
    38161 gccaagaata ttgaacagaa taatggacgt ttaccgttaa cagctcgggt gattttttga
    38221 tacggcatat ggcatttaat atttccccgc tctcaacatc ttatcaatat ggataagcgc
    38281 gttcttctcg cggggcgaat atatgaaacg gtgccctgtg tgtcaaggct tctgttgagg
    38341 tgtaaagcac tcgtgatgat tttcttctgt gcacgattga gttttgaaga ggtgaagtat
    38401 ggtaaagaaa aggcacatgt cctttgttac tcgaattgat tttctcaata tgcaaaaata
    38461 acgtcataag atttcatcca gaatcaatct gtcgatcttc gacatgggca acttttttaa
    38521 atcgacaggt gatacaaatc gcgagttctg gcacccgata ggtttgggag agcttttcaa
    38581 caaacggcaa ttgtaggcgt gaagggttat gcggaagtgc gtatacgtat gattgaccga
    38641 ggccagatgt ttttccgtct ggatggaaat gccaagttct tctttaacgc ttcgccttaa
    38701 acttctttca atatcttcac ccgtttcaac gaaaccgccg ggcagtttcc acaaagatgc
    38761 caataatcct gatgcagggc gttgaacgag cagtaacctg ccttttgcat tgcggatgac
    38821 ggctgctgct gcctgtcgat gcgggattgc gggagtcttt ttgattatag gcaaaacgtt
    38881 ttgtaaactt ttgattcgag cctgacaaag gttggccacg ggacaacggg aacagtcggg
    38941 atttttggct ttacagattg tcgctcccag atccatcagg gcctggttga aatcacccgg
    39001 atggttgacg ggaatcagcg acgcggcgag ctcctgcaat tgtttttgtt cccgtgcgtc
    39061 gtccaccggt ttgcggatgg caaatacacg gcacaaaatc cggcggacat ttccatccac
    39121 ggcaggaaac gcctgtttat aagcgatgct taagatggca ccggatgagt attggccgat
    39181 tccggggaga gtttttattt cttccaattt gtcagggatt tgcccaccga atttttccac
    39241 aataaccttg gcggcagcgt gaagatttcg ggcccgtgaa taataaccca gattttccca
    39301 gactttcagc acatcctgca aaggggcgcg agccagtgag gaaacggacg ggaatgtttc
    39361 cagaaaacga taatagtatg gaattaccgt atccacctgg gtttgctgaa gcataatttc
    39421 cgaaatccag atacggtaag gatcatttgt tttgcgccag ggaagcaagc gttgattgcg
    39481 gttgtaccag gccagcagtc ggcggttcag aagttttttg attttgtcgg tatccttcat
    39541 catcgtggtc tgtatatcct ataccagcga aaggaatcaa atcttaattg gcgatatttc
    39601 atgtctgcgt tgatcatcag gcaatttcag gacaatattt taggcgagga atcctgatgc
    39661 gttaagattt cttttccgaa aggcagtgtg ttgacttttt aatataatgt ttgaagagga
    39721 gaaaaggcgc cgccccggtt taatcctgga gcgacacctt ctcccgcctg tggtgttttg
    39781 gttatgccgc caatgcatgt tccggctgcg ggtgaaccag tggcttatct ttttttaatc
    39841 gcctgcaaaa cctcgtcttt ttcgagcggc tcgcccgcgt attgttccac atgctgataa
    39901 acgatttgtt tgcccggacc caggacaaat accccgcctt gttgccagag gtcaccggcg
    39961 ctcaagccct ggtggaagcc gctctgcatg gctctaacac cgcacttgag tgatttccac
    40021 cccagggtct tgaagaatcc gcgctcaagc gaaaaggcat tgtatgtctg aagagattca
    40081 tcgacataga tatcaagttc gacgttgaaa ttctcggcaa atgtctttgc ctgctcgggt
    40141 gtgccgctcc cgataatcaa gatttttgct ccgtttgcct cgatttgttt ttttatctcc
    40201 attaaggcag cagcccgctg cctggcgaac attcacccga aatggcgtat aaacaccagc
    40261 acaacgcgat tgtgtttcca aagggattca aaagcaacct cctcgccctt catgttcgtg
    40321 acggagagat ccgcaatgcg tttttccaac tggggataat cgatagcagc agttatttcc
    40381 tgctgatcgc ttgcccagcc gatgatcggt aaacacgtca ccagcaaaaa tgttaaaaag
    40441 tatttcttaa agtgattcat gcaacacgtc tcctttctta cccgtattta attttttcca
    40501 acgattgcgg gtttgcatca ttcttccccg aggttgaaaa tcgctttgag atcctcctgc
    40561 ctcaggatat cgtccagcca ggatgtaaca tccggcggat cataaaaagg ctgctccctg
    40621 aataacgcca ggaagggttc atagtaggac aggtgaaacc tttcgagccg ggagcccagc
    40681 atgtccttca ccatgcgcga gttcgggcgg tgatacctga gtattgcgga atccagcatg
    40741 cccaggtcga gaaaggactt cactttgtgc gagcagcgac acttgttggc ggggttcata
    40801 tggccgcagg ttccgttcat gtaattaaat accttgcgcc ggctgcgcga aagcagttgc
    40861 cggaaatttt cccgcgatat atccatcaac tcgcttccga cggcatcgga cacggcaaaa
    40921 acggctccca gaagaaaagc aattctctcg gtgcgcttca ggcacagcag catacccatc
    40981 atgcagccgg ttttgacttc ttcggccaga aggcccgcgt cgggatgcgt aaatgagctg
    41041 tcatccggaa aattttcaat ggcggcaatg tagcggtcga agtcatagat ccgcgtttca
    41101 aacttccttt ttttcatatt aagcacatga ttgaccacaa tccggtaggc ccacgtcctg
    41161 aaatttcctc tgtcggggtt gtaggatgcc agattcgtga tgattttaat gagtatttcc
    41221 tgggtgatgt ccgccgcgtc gtcatgatcc atgatcatct tgaacgcgat gttataaatc
    41281 catggctgat ggtcggccac cagttgctcc agggattttc tgttgccgtc cagagcatgc
    41341 tgaataagaa tagcatccgg attgtcattc ccggtatttt ggctcaaggg gttgagcatt
    41401 atgtttttct gttcgtttga catactccac ttatacaatt gcaattcacc tctgtgacag
    41461 aaaaaatcat gttcaggtga ttatctacta aaataatatt attatttcac tattttaact
    41521 ccagaggctg cgttgtgttc ttttcccggc aggagaatat gaaatgaaat aaaaagcaag
    41581 acaaacatga gtttaaaagt gcaaggcgga acctgaagag ccgggggcgt aaatgcgggt
    41641 aaagacggca cattatcctt tttgtcttga cttattcatg gccaatcttt aaagtttaac
    41701 gaaactcgtt gagaacgaac gtaatgatgt ttgtcccagc cacccgaagg tggcgggaca
    41761 attcgctctg ggattttccg gcgctacgtt tcgaaaaata acggtttatt aaagcatcag
    41821 tcgaattatc cccgaagcgt tgcaaggggg aggggacact tcacatctta acaggagatt
    41881 aaaatggcaa gtgatttaaa agcggttatt gaaaccagca aggggacaat caatttaaaa
    41941 ctatttgcag atcagacgcc ggttacggtg ggaaattttg tcaacctggc gaggcgcggt
    42001 tattacgatg gtttaaaatt tcatcgggta atcccagatt tcatgattca gggcggatgt
    42061 ccatttggcg acggccgcgg tggacccgga tacagatttc aagacgaatt tgtcaaagaa
    42121 ttgcgtcaca ataagtccgg tattctgtcg atggccaacg ccgggccgca gaccaacggc
    42181 agccagtttt tcatcacgca cgtggatacc ccgtggctgg acgacaaaca cacggtattc
    42241 ggtgaagtgg tcagcgatgc cgatcaggct gtggtcaaca aaatcgccca gggcgataaa
    42301 atcatctccg tgaaaattga aggagatgtt gcgccgctgt tggaaaaaat caaaccggca
    42361 ctggataaat ggaatactgc cctggatgaa aatttcccga agcttgcgaa ggtttaatgc
    42421 aataaacaag acgcgatatg agaaaagtaa cagaaactcc ctcaaagatg gtggaaaaag
    42481 acggaacgat tcatttcggg acgtttcgca cgccttttcg cgatgtgaat attcttgacg
    42541 tgccgcttta ttcgtcttca tccggcgttc ccgcattctg gaagaaattc cgtctcaagg
    42601 aatggcagca tttcggtatc atcacgtcca actattattt tggcatggtg atctttgacg
    42661 ccaagtttat gggagtctcc tttttttatg cctacgacag aaatgagaat aagcgtttcg
    42721 aacacagtgt gcaattaccg ggcggtgcgg cgaatgttgc tgcacaggtt tatgatggcc
    42781 actgcgaatt caaaaaaaaa ggttaccgac tttatattga aaacagattg caacagggct
    42841 atcataaaat catcatggac atcgacggca aaaaagatct gccggctgtt caaggggaaa
    42901 ttaaagttct ggaagacctc aatgggatag aaccgctggt gcaggtttct ccggtcacgc
    42961 ctttccgtcc tttttacacg cataaggttg ttgcgccggc ggaaggtgaa attatgctgg
    43021 gcagggatag catcaaggtt aaccgggaat gggatgtcgc cctgatggac gaacagaaaa
    43081 catattaccc ttatgtcagt ttctggaaat gggcgacggc cgcggggtac aacgaatacg
    43141 gagagctcct cgcttttaat ctctgtcaga acatgattac cgatgacggg aatttcaacg
    43201 aaaactgcat gtgggtggac ggaaaaatat actgcctcaa cgcggcgcga tttaaattcg
    43261 atgaacagaa tgtcctgcat ccctgggaag tcaaaacgaa tgatggatgc atggatttaa
    43321 tatttacgcc ttccggtgaa agagccgaaa aaatcagcat cgggctgatc gtatccgatt
    43381 ttcaccagcc gttcggcctc tataacggac gattcaagga caacaacaac agaatatatc
    43441 cgattaccga ttttttcgga ttggccgagc atcacgtgac aagatactaa atagtgcggc
    43501 attttttttt gcatatgttt gatatcggga atcaggatca aaaagccacg cagtatcctc
    43561 tgcgctggga tcgcttctgg gcaagatgca tagatttttt tttgcaagcg gtattatgtt
    43621 tcttgatatg gattttctct ggaattggtt cttgggtttc aggtacaata cacaaacgag
    43681 atctgcagga tattagtcat ttaacaaatc ctcatttctg gcaagtttat ctcatctttt
    43741 tcgcgatgat gtccttagca ttctttcttt acgaggtttt ttccgtaagt gttttagggg
    43801 caactccggg taaaatatta tttaaaatca agattgcaga tcgtgaagga agaaaactta
    43861 acttttataa tgccttcaaa agatcaattt atcttttttg gtttggctat tatttttaca
    43921 tttttacttt tcacggtttt attattggtt actggctttc cagtcgatat tataaaaaaa
    43981 gaggcacttt ccggtgggat caagcgagcg atagcattat aaatcaagaa acattgacac
    44041 gaacacgcaa gagagtttat agatttctgg caatgtgttg tcttttcgtc tgtatggtgc
    44101 cggttccgga gattagtttt tcgattggtt ccttgatagg gaagttagca ttataagaag
    44161 tatttgagat aaaagtattg ttttgatctc ttcagttgtc tggatgtctt ttaacgataa
    44221 atttttctcc catagccatt cccgtgcgtt caatatatct gtaagataag agcgatataa
    44281 tagtgagtaa aaaaagtgta atcagtagcg acgataaata tcccagcatg tctgtcggaa
    44341 tgaaattgta gatccggcga tagacgggaa tcacggtaaa aataagaagg ggatggaata
    44401 agtacatcga gtaacttgtc ctacccaagg aagcggtttt gtcgttgacc agaaaattaa
    44461 aaggcttcaa tcccaaaccc agcgccagca aagaaaagca gatgccgtgc agaatctttt
    44521 tcccccacat gatgttttgt atgttttcag taagcaggac accataaaaa aatataaaag
    44581 caagaatgaa tagcatcccg taagtttgcc gggacttttg gtttgttttg ctgaagtaat
    44641 caaagaacag gtgatagacg ataatgccgc aggcaaatac cggcaggtgt tgaaggaaac
    44701 cgaaccccca gacgaagccg acgtcatccg ctttcagata tccggttgcc acgccgtaat
    44761 atttgacaaa atacgaccag ctccacgagg tcagaatgga aaatataaat aaagccagcg
    44821 ccccgtgcag gttgcggatg tatctgtgga tgacagggaa aatcagataa agtacggtaa
    44881 cgacaccgat ggcccagctg gcccacacaa atccggttat acaggatggc accagattaa
    44941 acaaaagcga ggcgttgacg agaatttcag agaaaggatg aaatacacca aaaacagcgg
    45001 catcgcgaat ccagtacagc agcatcagca aataaaacag aggcgcgatt cggaaaaagc
    45061 gtctgacata aaaattgcgc agaagccgtt tctctttcgc gcgtttgtcc atggcatagg
    45121 agagggagaa cgcgctcaag acaaagaaga gactgacgcc ggcgtcgccg ccgttgatta
    45181 aaaacggcgc aagccattcg ggaattgcga gattaggttg aggaatcagg atcaggtgac
    45241 tgatgaccac ataaatgatt gcgaaacagc gcagggaatc cagaaagccg aggcggacat
    45301 agggcgtggt cgtagaattg tttggcatta aacgctccgt aaaattggag tattccggca
    45361 aaagaaataa aaaactcttt aagcgtcttt tcgtatcttc ttcatgtgat cgatacgttt
    45421 ctggatgagc gcgtcggtgc cgatgtcttc gcgataagca acgggacctt tgacggcctt
    45481 taccccttcc tcggcaattt tccgggcatc cgccagatta tcggcaattc cgacaatgcc
    45541 gatggcgcgt gaagagctca aatacaagcc gtcatccttc tgatccacag atgaataata
    45601 tagcctggcc cggcccacat tccccacttc aattttcgcc ctggatgagg cggcatcggg
    45661 atggtcggcg ggtaggccgt agcccttcgg cacaacatat ttgcaaaccg tagccttctt
    45721 atcaaactct atttctattt gatccagagt tcctgcaatg atgtgccggc acacatccac
    45781 gaaatcggtt ttgagaagcg gcagaatatt catggcttcc ggatcgccga accgtgcgtt
    45841 gtattccaag agcttgacgc cgtccttcgt ggcgatgaat cctccataca tgacgccttt
    45901 gtagggactg cccgtttctt tcaaaagtac ttctgcgacc tgacgggtga tttccagtcc
    45961 gtcatgcaga tcctgcggtc tcaaaaaagg catcgaatgg tccgccagtg aataagagcc
    46021 catgcctccg gtattggggc ccttgtcacc gtcaaatctt ctcttgtgat cctgcacgag
    46081 cggagtagcg acaactgtct tcccgtcgca caggcattgc agcgaaaatt cttccccgtc
    46141 gaacttttct tcaatcacca gcgaagcgcc tccggccagt atctgtgcgc atagtttcaa
    46201 cgcttctttt tttgaatcaa aatgatctcc ctggacctga acgcccttgc cgcccgtcag
    46261 tccgtccggt ttcaaaacga tgccttccaa ctgattgaga aaatcttcga cgccatcgag
    46321 cgttttaaat actttaaact gcggattacc gggaatattg tatttgctga ctagattcct
    46381 ggtgaacgat ttggacgttt ccagccgggc cagcgatttt gtcggtccta cggcgggaat
    46441 atttattttg gccagcgcgt ccgcaacgcc gttatttaaa ggatcttccg gcccgattac
    46501 ggcaaattcg attttatttt ccgtggcaaa ttttgtaatc gcgtcaagat cggcataaga
    46561 accaatcaag gttttttctg aaagagaagc gatgcctggg ttattggatt tcatgaaaga
    46621 gaataagctg ggcttttgtc cggagcgtgt aatcgcttca gccagtgcgt gttcgcgggc
    46681 gccgtttccg agcagtaata tattgggcat ggggatctcc tgtcgggctt ttggattttt
    46741 cgcttttata aagagaatag attgggctgt caatggaata ttcaaacgaa ggcaatgttt
    46801 ctttttttga cagaatctgt taaattaata accaaggtta gaaatcttgg tataacttct
    46861 cttgggtcat ttcggggagc aaagcgacgg gaaatctaca ttcaatatta ttgccggatg
    46921 atagtttttg gtcgaaaaga cagtttgata gttatgcaaa agtttcaatt catgagatga
    46981 ggtgtctggg tattgggctg gaattcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47101 nnnnnnttat gcaaaagttt caattcatga gatgaggtgt ctgggtattg ggctggaatt
    47161 ccgacatagt tgttttaatc cttcttttca cgctttccgg atttttttcg tcatcggaag
    47221 ccgctttgct ttcactgcgg gatttgcatc tgcataagat gcaacaaaac aattaccctt
    47281 tttttaatta cgtcgtcaaa ctgctttcca accagcgccg gttgttgatt acaatcgtaa
    47341 ccagcaatga agtggtcaac gtcagcattt ccatcctggc ggcgtctttt tttatcgggc
    47401 ttttcggtag cagcgggcaa tgggtgtcga ttgtttttac aaccgtcgtt ttgctttttg
    47461 tcggcgaagc tattcccaaa acttttggcg taacctaccc gatgcagatt tcctcgctgg
    47521 tgtctccgct cttgatggcc atttcgcgaa tcgagtctcc tatcgtttca gccctggaaa
    47581 aagtatccgg tcttttcctg cggcgttacg gggttaataa atccgaggaa tcggaagtca
    47641 ttatggaaca cgaattcaaa acgcttgtcg atgtcggaag tctcgaaggc gttgtgaatg
    47701 aggcccagaa agaattgatc aataaaatat tcgaactcgg agacaggccg gtgacggata
    47761 tcatggtgcc ccgtgtggac atgttttgtc ttccgctgga tatgccggtc ggggagatgc
    47821 ttcaatccat cgccgccgcc cggcaggaac gggttccggt ttatcgggag gatagagacg
    47881 atatcgtcgg aatcctgttt gcgagggatt tgctcaataa tgtctggcgc ggcagatctc
    47941 agaccggtat ttacaatctt ttggtcagac cgtttttcgt gcccgaacaa aaaacaatca
    48001 atggtctgct gcacgatttt cagaaaaatt ttattcagat cgccattgtg gtggacgaat
    48061 atggcggcgt ttccggcatg attacgctgg agcatattct ggaacatctt tttgaagagc
    48121 cggaaggtga tgaggaaatg gtttgcgacg gttgtgaaaa aatcgacgag caatcggtta
    48181 ttgtccccgg gagtatgtcg gtcgagcagt gcaataagct tctggaagcg gaattgccgg
    48241 aagacgagtt tgacaccgtc ggcggactgg tgcttcatct tttcggtaaa ataccgcaga
    48301 agggcgaaga ggcgcatctg gacggatata tcttttgcgt gcatgatgtc ggcaaaacaa
    48361 gaatattgaa ggtcaggatt acgaaaaaaa cagttgagga acctgaagag tcatgagcct
    48421 atatttaatc gtcgcagcca tattgttttg tctgatgctg gaaatgcttt attccggcgg
    48481 tgaagtagcg ctttttgctt ccgatatcaa taaactgaaa aaccgagcgc atcaggggtc
    48541 aacatccgcc caccaggccg tcaagctcaa agaaaagccg gaatggttta tctcgacggc
    48601 acttgtcggg accaatctgg cgatcatcat cgcttcaacg cttatcaccg gtcttttaat
    48661 ttctttttac ggtccggcac gcggcgaaaa aatcgctttc ctgatcatga ttcccacgct
    48721 gtttctcatg atcattgccc gcagcatatt tctttactac gcggaagcca tggcgatgcg
    48781 tgttgcctgg tttatccgtt tttcatcgat acttttttat ccgatcgcct tcgtgatcgc
    48841 ggccgtttcc agaagcacgg tgcatttctc ttccgatacc agaagcgggc agtcctcgca
    48901 cattaccaag gaaggcctca aatacatctt gggcgaaaaa acaaaagggg gcgatatcct
    48961 gacgcgtgag aaggaaatgg tcagccgggt gtttgatttc tccgaactta cggccatgaa
    49021 aatcatggtg ccggtttccg ctttgacggc gctgtccgct tccatgaaaa tttccgaagc
    49081 cgtaggtgtt gtggccgata aaaaatacat gcgcattccc gtttatgaag ataccgttta
    49141 caacatcgtc ggcattttac gctacttcga tctttttgaa atgctcctca aggacaagga
    49201 gaaaggcgaa ggtgtcggcg aagcgacggt tgcctcctgc atgcgcaaag atgtctttta
    49261 tgttccggaa acaaaaaagg tcagcgaact gctcgcagca atgcaaaaaa gagacgagca
    49321 gatggcggtg gtggtggatg aatacggtgg cgcgaccggc gttgtcacca ttgaggatat
    49381 cggcgaagaa atcgtcggaa caattgatga atacgtgtcg ggagaaaagc tttacagaaa
    49441 aattgctgaa ggacgttatc ttgtgtcagg caggctggaa ataggaactc tgcggcaatt
    49501 ggttaaagcg aaattccccg aagggaatta tgaaaccgta ggcggctttc taatgtatca
    49561 gatgggtaaa attccccgaa taaaagaata ttatcagtat ggcgatgtga cgtttattat
    49621 tgaaaacgcc gaccagaaat cgattaagga agtgacggta atcgcgcctt ttattcaaga
    49681 ggggaacaaa taaatatctt tttgtcattc ccgcgcaggc gggaatccag taaatgtttt
    49741 tgttcaaaaa agattattaa taattggagt gattttgata agtgtagatg agataatcaa
    49801 ggattattaa atgaaatcag atttaacaag acaagattgg gaagagtatt taatccgtct
    49861 ctattttggg accaaaaatg atttaataaa atcatgtatc aatagagcct atcgcgattt
    49921 caatagaacc cttcatggaa tggatgatca caaaaataaa caagatttat ttgatagagc
    49981 cgtaaaatat ctgctctcaa ttttcataga tataaaaaaa gagtcattaa atgaccacga
    50041 agaatttgat aaatggcata aagaaaaatg ccttcattta aaaaatttat ataaagaaaa
    50101 tgatttcatt aatttttcca ttgggcaggc acagaaatgg ataaacatga cttttaagta
    50161 tatattttgt gtgggtgaga aaagattacc cggttattct ggtttatatg agttttgtca
    50221 tgttcctatt gacaatataa ttttagataa aattaaaaaa agaggtgctc ctgtcccgtc
    50281 aaaaccgtgg agtcgacttg gtgattataa cgaatatatc tctctccaga agtggttccg
    50341 agaacaatat aaattaccat tggatggtga atttatatta tggatggagg aacctcatga
    50401 taatgtcatt atgcctgttc aaaacaatat tgttttggat aaaaccagag tcaaagaagc
    50461 aaaagacgag accataggac catatcaact taacagaata attctgtgct ctgccgatgc
    50521 gagtccggca tcacaaaaga aagacaaata taaagcagaa tatttcttcc caagcgcaaa
    50581 gtgggttgga gccgttagaa atgtcgcaag tgatttggga tgtaaatttg ttattcttac
    50641 gaccaaacat gggctggtta atccttccga tgatattgat cattacgatc tagaaatcaa
    50701 aggtaatgaa aatattatta atgagaaatg gcaagataca acgtcaagat taattagaag
    50761 aggacaatat gacattctag ttttttacgc aggtggatgt cctcgggatg aatacttgag
    50821 aatattaagt ccgattctta agaaccttga aatagattta ttaacgtttg ggaagcccaa
    50881 catgggagat gcgggaaaaa ttaaagacat cgtcgaatcg ttgggggaag aagcatctat
    50941 agatacattg aaaaagaaac ttaaattccc agatagacta ttatttttcc cccacaataa
    51001 taaggcataa ttaattttaa aatactggat accggcctgc gccggtatga caaacgaata
    51061 aggattgata aggtgtagga aagtgcgttg atattggcgg cggggaaggg tacccgcatg
    51121 aaataaatcc gggcttgcaa aattttgcaa agtatatata attaacaaaa gtgataacta
    51181 tatatgctta gtaaaaatat ggcagaattg caggaattga gcaggaaaac ctgctttacc
    51241 ctcgaagatg tggcgcagag tttgggaatg caaacggcat ccgcccgcgt attgtgcagc
    51301 cgctatgtcc ggcagggggt gctgataaga ttgaaaaaag gtttttatac caccgcctgg
    51361 aagtgggaaa atcttaatcg tcaggatttt ttcaaaatcg ccaatatcct gcaggttcct
    51421 tcctatattt ccctgatgtc ggctctggct tattatgaag taacgacgca ggcgcagaac
    51481 aactatcagg aaaatatttg cctgaaacgt tcgattgttt acaatgtaag ggaagcggtc
    51541 ttcaattatg tcaaagttca ggcccgatac tatggtgatt ttgttaaaac ggatggcatc
    51601 ttcatcgcca ctaaagagaa agcgtttgtg gacgctgcct atctttattc gttcggcaaa
    51661 tataagttcg atgttgattc tctggatatg aagaagctgg atatcaagaa gctcaggagc
    51721 atcatcaaaa tctatccgca aaaaacaaaa gagacggtta aaagattatg ctggatttga
    51781 ttcaacatga acaatttgag ctggaagttt taaacagact tcaaagcggg cggtttctgg
    51841 ccaacttgat tttcggcggc ggcaccatgt tgcgcttgtg tcatggactg aatcgttatt
    51901 cagttgatct ggatttctgg gtagttaggg agatggattg ggcaaaatac tatagccgga
    51961 tggaaaaata tctgggccag ttttacaaaa tagccgattc cgccaataag cattttacaa
    52021 ttctgttcga attgaaatcg ccgcagtttc cacggtcgct gaaaattgag atacggaaaa
    52081 cggtaaagaa cgtcaagaca gaaacgagca tcgcctacag tccgcacgcg ccggttcagg
    52141 tgatgttgaa aaccgtggcg ctaaaagata tgatgacttc aaaaatcgaa gcctttcttg
    52201 accgccggga aatacgcgac gcatatgata tagaatttct agtaaaacgc gggattgttt
    52261 tgaatgccga tcatgaggtt atcacccaaa tgcttacgaa actggggaaa ttgggcaaaa
    52321 aggatttttc agtaaagcta ggttcgttgc ttgatgtctc ggaacgaaag tattatgtgg
    52381 aaaacggatt ccgcattctg gaggcgaacc tgagggataa actcaggtag aatttaaaat
    52441 acaggatacc gaccttcgtt ggtatgacga ttgaaggaga tgtttgataa ggtgaacgaa
    52501 acgaaatttt gtgcgttgat actggcggcg gggaagggta cccgaatgaa atcggataag
    52561 gccaaggttc tgcatgagtt gaacggaaag cccctgcttt attattccat cgaagcggca
    52621 agagcggttg gcgtcgaaaa gattgtagcc gtgatcggcc atcaggcgga gagagtcagg
    52681 gaaacatttg ccgccaacgg ctgtattttt gtcgaacaga atccgcagtt gggaacgggg
    52741 catgccgttt tacaggcgaa ggatgtactg actgattaca aaggctttac cgtaattctt
    52801 tgcggcgatg tgccgctttt aaaaccggcg acaatcaaat cgctggtgga taatcatgtc
    52861 gccgaaaaag cggtcgtttc tgtgttgaca accattccac cgcatcctca tgcctatggc
    52921 cgcatcgtaa aggacagcaa gggcaatgtt ttgaaaatag tcgagcatag ggatgccacg
    52981 gaagaggaga aaaaaatcgg ggaaatcaat accggcattt attgtgtgga tacagagttt
    53041 ttgtttcatg ctttgggaaa agtgaccaat aacaaccggc aacaggaata ttatctgacc
    53101 gatattgttg aaatagccgt ttgtgaaggc cagaaggtaa aatcatttat cgctgacgat
    53161 tacgttgaag tgatgggaat caacacgctg gaagaactca cccgcgcgga aaagtatctc
    53221 cgaaaaggct gaaaattttc tgttttattt gtttctgtcc tgatattaac attaaagtga
    53281 aacaattatt tttcggtcat accggtcgca gatgaccttc aggttacgaa ggccggccat
    53341 ggtgccccta tagggtatat ccagttattc ttggatgccg ttcttcaacg gcatgacata
    53401 gttgtgcgtg atattttatt tttcagtcag ctaggtaatt attggtttga aaaatataaa
    53461 caacataatc gaagcagtcg caaaaaaagg agctctgctt gcgcctctgg cgggaatttc
    53521 cgatttacct ttccgtttaa tcgcccgatc tcaaggatgt tccctggcct atacggagat
    53581 gatcagttcc aatggtcttg tccgaaacac cagaaaaacg tacgagtatc ttcaaacctg
    53641 tccggaggac cgcccgctcg gagcgcaaat ttttggcgct gatccacagg tcatggctgg
    53701 ggcagcgagg atcgtggcgg ataccggggt ggatttaatt gatatcaaca tgggctgtcc
    53761 ggtgaaaaaa gtgattaaaa ccggtgccgg agctatcttg atgaaagatc ccgatcttgc
    53821 tgcccggata attaaagcgg taaaaaaagc cgttattatt ccggttacgg taaagattcg
    53881 ttcaggatgg aatcagtcgt ctatcaatgc tgtgcaaatt gcaaagatag cggaggattc
    53941 gggagccgac gcaattacca tacatggccg cacggctgac cagggttaca gcggtcatgc
    54001 cgattggaaa atcatcgcgg aagtcaaaaa agcggttaaa acactggtca ttggtaatgg
    54061 ggacatccgt caaccgcagg atgcagtcca aatgcttgct gaaactgact gcgacgcggt
    54121 gatggtgggg agggctgcat tgggcaatcc atggattttc aaaggaattt ctcaagcact
    54181 gcggggaggc gaaattgaat gccatccctc gctgaatcaa cgtcgggaaa tgattgaaaa
    54241 tcattggaaa atggaagccc ggatgctcgg tgtcaaactg gccggaaaaa gttttcgtaa
    54301 gcatattctc tggtatacca aaggtttgga caattcacac cggttccggg aaatggccgg
    54361 caaggttaat aacgaacaag aaatctttga ggcactaaat aattattttc atttgctgaa
    54421 tacggaaaaa atctaaaata aggacttgac attaatgcga taaattggca taaaataagg
    54481 aaaaagaaac tatggaaaat catactttat ttaatgaagg aaaggtaaat tctactgtgg
    54541 aactcgaaaa attttcaaag ctcgaagaga aaattaaagc tattgttgaa gagaaagttt
    54601 ctttaaaaaa tcaaattaag acattggagg atacattaaa aaataaagag gcggaaatag
    54661 aaaagttgaa cggaaagata aaggtattgg atgaggaaag gaacagcgta cgcgcgaggg
    54721 tagattcact gcttgattta attgcagatg taaattcagc aaaataagct tccagggcgt
    54781 gtgttttgaa aaacagttac aatataacaa tattaggcca ggaactttct gttttgagcg
    54841 attcggagga tgagcaggtg gccgaagttg ttcagttttt aaataaaaag gtggatgaga
    54901 tattacattc gggcaacggt ttgaagacat tgaacgttgc catcctggca gctttgaaca
    54961 tttcagagga gtttttaaag ttaaaaaaag ttaacaaaga gctctgtgac aaattggaaa
    55021 acagggccga agaattaatt cagcttatag aggaaacaag ttagaaactt tttttattag
    55081 cccctgcgat gtgcgtgatt gacatttgtt tttgagccaa cattttgacc ttgggacctt
    55141 atccttgatt gtggtgtgca tgtccgcttt ttcctgagct ggtcgggaag cagaaagcct
    55201 gaaacaacca ataccggttc ccaccttgtt aaaaggttca aaaaaggtga ttaacacggc
    55261 ataggcgggg gtctttttta tctgttcatc aacatcaatt ctttccagcc tttagaaaat
    55321 caataaaaac gccaaacacc gaggtgaagc atctgaacca ttgatgcgcg ccgtggttaa
    55381 atatagctca accggaaaag aagtttatat tcttgaaatt gactgtgatc aggtcgggga
    55441 atgaggtttc cggaagggca ccaaggaggt aacatgatat gtttgatgcc agttttttat
    55501 cagtgctggt tttagttgta gcggttgttt cagggatcat tatcggcttc attttgaagc
    55561 agtccattgc ggccaaagaa atcagatcat ccaagaattt gatggctcgc atggcggaag
    55621 aggccaagaa agaggctgaa acaatcaaga aggaagcggt tcttcaggcc aaggaaaatc
    55681 tcctgaagat gaaagccgaa tttgatgctg aatcgaagga aagaagaaac gatttcgaag
    55741 cgcgtgaaaa acgcattcgc accaaggaag aaaatctgga taagaaaacc gatgctctgg
    55801 cgcaaaagga gtcgtcggtt gaatccaggg aaaaatcaat cagcagtaaa gagcaatccc
    55861 ttgaagacaa acgtcgtaaa ctggatgcag cgcttcatga acaacaggag agactcgaaa
    55921 agatagcggg tatctctccc gaggaagcga aaaacctgct gattaaatcc atggaagcgg
    55981 aagccaaaca ggatgccgca gcgcttgtcc gcaagattga agacgatgcc aagctgaccg
    56041 ccgacaaaaa agcacaggaa attatttcct atgcgattca gcggtatgcc ggcgatgtag
    56101 tggcggaaaa aacagtttcg gcggtcagtc tgcccagcga agaaatgaaa gggcggatca
    56161 tcggccgtga gggccgcaat atccgcgcca ttgaagcggc cacgggaatt gatttgatca
    56221 ttgacgatac gccggaagcg gttgttttgt cgagtttcga tccggtcaga cgcgaggtgg
    56281 cgagaatttc gctagagcgc ctgattcagg acggacggat tcatcccggc cgtatcgaag
    56341 atatcgtcaa gaaagtcagg acagaggtcg agcagatcgt cagggaaacg ggtgaaaagg
    56401 catcctttga cgccggtgtg cacgacatcc atccggaaat catcaatctg ctgggatcgc
    56461 tgaagtaccg taccagttat tctcagaatg ttttgcagca ttctattgat gttgcctatt
    56521 tgaccggtat catggcgtcg gaactgaaaa tgaatgtccg ggaagccaag cgtgccggtc
    56581 ttttgcacga tatcggcaag gcaatagacc ataaagtcga aggcacgcat gcagccatcg
    56641 gcgccgatta cgccaagcgt ttcggtgaaa atccgcgtat tgttcaggcg atagcaacgc
    56701 atcatgatga cggacgcaac aataccctgc tcggcgttct cgtacaggcg gcggatacgc
    56761 tttctgctgc cagaccggga gcgcgtcgcg agatgctcga aacatacgtc aaacgtctgg
    56821 aagagctgga aaaaattgcc aattccttca atggtgtcga taaatgcttt gccatccagg
    56881 ccggtcgtga aatccgtatt cttgtggaaa atgaaaaaat atccgaaaat gatgccacga
    56941 tgctgtgtaa ggatatcgtc aagaaaattg aaaacgaact tacttatccg ggccagatca
    57001 aggtgaccgt catcagggaa acaagagttt ctgattttgc caagtaattt ttccggtatg
    57061 ggaaaggaac gatgcaaatg aaaattttat ttatcggtga cattgtgggc agccccggcc
    57121 gcatggcggt cagggaactg ttacccgacc tgaaggccag aaaaaaaatt gactttgtca
    57181 tcgccaattg tgaaaatgga gccgccggat tcggggtgac caagaaaatc atggaagaac
    57241 tcttcaggga tgacattgac gtgctgacat ccggcaatca tatctggaac aaaagagaag
    57301 tcctggagtt tatcggagaa tccgaccggc ttttacggcc ggccaattat ccggccacgg
    57361 cgccgggcgc gggagcggtt cttgtccctt tgaaaacggg cggctatgtg ggtgtcctca
    57421 acctgatcgg acggatcttt atgcaggcgg tcgattgtcc ttttatcacg gcgaaaaaca
    57481 aagttgcgga attgagagaa aaaacaaaag tcattattgt agacatgcat gcggaggcaa
    57541 cctccgagaa aaaagcgctg ggctggtatc ttgacggcga ggtatccgca gtcttgggga
    57601 cccatacgca tgtgcagacc gctgacgagg aaattttgcc gaacggaacg gcgtatatca
    57661 gtgacgcggg catgacgggg ccttttgatt ccgtcatcgg tatccggaaa gaagctatta
    57721 tcgagcgatt tttaacccag atgccgaaca agttcgattt ggcaaaagac gatttaagaa
    57781 tgcagggcgt cattatggat attgatgttg acagcggcaa ggcaaaatcc attgatagaa
    57841 tcagcgtcaa actgaaggat tcgtaagagg ctggatgcaa tgaaaaatgc ttacgatatt
    57901 ttaaaagaaa gaggtttcgt cgagcagatc accgatgaag gcttgattca agaacttttt
    57961 gcgaaaggtc cggtgacctg ttacatcgga ttcgatccca cggcgaccag cctgcatatc
    58021 gggtcgctgg tgcccattat gtctctggcg cacatgcagc aaagcggcaa caaacccatc
    58081 gcgctggttg gaggcggtac cggcctgatc ggcgatccca gcggcaaaac cgaaatgcgt
    58141 caggttctca cccgcgaaca gatcgactac aacgccaatt gcatcaaaca acagctttcc
    58201 aaatacctcg acttttcgga cggtaaggca cttcttttaa acaatgccga ctggctgacg
    58261 aaattaaact acatcgattt tttgagggat gtcggccgcc atttcagcgt caaccggatg
    58321 ctggcggcgg aaagctataa aatcaggatg gaaaaaggcc tgaacttcat cgaattcaat
    58381 tacatggttc ttcaggctta cgatttcttt catctgttcc agaattacgg atgtgcgctg
    58441 cagatgggcg gcaacgacca gtggggcaat atgctggccg gcaccgagct gatccgcaag
    58501 attgccgcca aagacgccca tgctgtgacg ttcccgctca ttaccacgtc cctggggcag
    58561 aagatgggga agaccgaaaa aggcacggtc tggctggacg gcgctttaac cagtccttat
    58621 gaatattatc agtactgggt caattgcgac gacgccgatg tggaacgatt tttaaaactg
    58681 ttcacgttcc tgccgctgga cgaaatcagc gctgtgaaaa atttaaagga tgctcaactg
    58741 aacatggcca agacggtgct cgctttcgaa gcgacaaaag ttacgcacgg agcaaaagcc
    58801 gctgaagatg cgtggcgcgc gtccgcggag gcatttcatt cccgtccggt ggagaccggt
    58861 atgtttccgt cctgcaacat tccccgcacg gcagccgcca gcgatacatc ggccatcccc
    58921 cgctataaga ttccccgaaa ggatttgaaa tccggcgtcc agatatgcgg agccttagcc
    58981 aaagctgcgt tgacgcaatc catctccgaa acgaaacgcc tgattgaaca gggcggtatt
    59041 tacatcaatg accgtcaggt gaaatccatt gatgaaaaac tgacgcttgc cgattttggc
    59101 gatgccgaag aaattcgcgt gagaaaagga aagaaaaagt atttaatcgt agaaatcgag
    59161 taaaaaatat ttccttgtgc ggcacagtcc tcttttctca aaagcataac tgaaatgcag
    59221 aaaaaaattg agagaaaaaa gaagcccttt cattaccggc acgcgaacct ccgaaggaaa
    59281 tccaaaagca acagccatgc tgctttttac aggcctgaaa tcgttcccgc gctgaaaagc
    59341 actttatcca ggatcggcaa gccgtatcca gccccttttg ttcccgatga atttcaggtc
    59401 aaagctttgg aggcaatcaa aaaaaccgat tgtctggtca tcgcgcccac gggttcagga
    59461 aaaacatgga ttgcgcggga agccatttta tccgtcctgg aaaaaggcgg acgtgcctgg
    59521 tacgcgtcgc ctctgaaagc gttatccaat tccaaatgga ttgaattcgg cctgcatttc
    59581 gatacggaaa atgtcggtat catcaccggc gataccaaag aaaacaccga ggcgccgatc
    59641 attgtcggca ctaccgaaat cctgcgcaat caactctacg atgccatgca tcagggaaaa
    59701 gacttgaact gcgatctggt cattctggac gaggcgcatt tcctgggcga caccgatcgc
    59761 ggcgttgtct gggaagaaat catgatttat cttcccgcgc ggattcacct gctgcttttg
    59821 tcagcaacca tcggtaacgg cgaagaaatt gcacactggc tggaatccat tcgcaagaaa
    59881 tcctgcgctg tcgttgcgga agacaaacgt ccggtgccgc tttatcccct ttttctgcat
    59941 ccatccggtt gtcttcatcc ttttctggaa gggaaaaaag tatccccgcc tgttgccgat
    60001 ttcatcagaa gaaccgatga gaaaagattg cgcggcggga aaatgccgga ttacatcggc
    60061 atcatccggg tactggaaaa gttcaatctg ctgccggcaa tattttttct gaaatcgcgc
    60121 gcggaatgcg atgcggcgct cactgcgcgc ggaatgctgc agccgcttca gaatgccacg
    60181 gtgttttcat attcactgga tgaactgctg ggacgtttcc ccacgctggc caggcaccgg
    60241 caattgaaag ttttaagatc gatgggactg gcgtctcatc acggtggaca gctgcccgcg
    60301 tggaaaatgc tggtggaaga aatgatgaac agaggacatc tgcgcgtgat ctttgcaaca
    60361 tccaccattg ccgcgggcgt taatttcccg gctcgaacca tcgtcctttt caactccgat
    60421 ttgttcaacg gcagtgattt cagtccgctg acggctacag aattccgcca gatgaccgga
    60481 cgcgcgggac gccgcgggca ggacaacatc ggcttcatgc tgacagttac cgggaaattc
    60541 atgaacctga atcacatccg gggtttgttg tttcaaaaac cggaggatat tttaagccgg
    60601 ttgaaaaacg atttcgcaat ggtcctgaat ctgcttttgt cccagacgcc ggacgatgtc
    60661 cgcaagatat tcgagcggtc gctcgcggca tatcagcaaa acagccggca tgaaagcgca
    60721 gacttttccg cggcccagtc gctctggaaa gatttttccc gccacctgaa atttctgaag
    60781 caggaaggat ttgtggatga atccggagcg ttgactgatg acggatgctg ggcgtcaaaa
    60841 ctgcgtctcg attatccgtt attggtggct cagtgcctgc gggaaaacgc gtttcctcag
    60901 gacaatgaaa agattctggc cgctcttgtg gcgttgttag cctatgatcg ggacaatgat
    60961 atgaaaatga ctgtaaaaga tttgccgccg aagcttgctc ttgcgtttaa aaaggtcatg
    61021 ctggccgtcc ggcctctgat ccatcggatg gaaaacgccg gatttgcgcc cgccagactc
    61081 cacacgctgg cgggcgtggc catgtatctc tgggcgcagg gatatggctg ggattccgtc
    61141 attaaagcca ccggaatcgc tgaaggcgat atggcaacgc tggttttgcg cacggcggat
    61201 aatctgcggc agatcgcatc gcttaaagat acatatcccg acattgccgg atgcgcgcaa
    61261 agggcaagag aaacgatcct cagggagccc gttctctttc tttaaagaga tctgttgaaa
    61321 gatcgcattc tctgaacgtc gccacgttag atatcgtttt gtatagatcg cttggtccat
    61381 gtttcgcaga tgcgtatgat cccgctcttg cacgcccatg gtgtcagtat tttttgaaca
    61441 gtccataaaa gtgaaaaaaa tttacagccg tcacggcaga gcgatgaccg gtgacgcgta
    61501 tcttttgatt caactttcat tttcaaaata ttgattttat gataaactta atacgtttga
    61561 atttgaaatt gaaatgatca tccatgatta gtgaagatct gaagacgttt ttttaacgga
    61621 ctagcgcgcg tatttgccat tgaggaaaaa cgcaagggga aacgatgtgg ctttatctta
    61681 aaacaggttt ttattcggta gtgaccaagg aaccgtgtaa agaagatgag cttttagtca
    61741 gagcgcacag taaagatgat ctggataaat tacaggattt actgaaaaaa cagtgtcaat
    61801 ttcacggcga ggtgctggat actccccaaa ccgattatgc ataccgtatg atcgttccca
    61861 aagaaacatt tgcgtcattt atgtcccgcg ccatcaatga tctggtttat gaagaataca
    61921 aaaacacaaa aagctgggaa gccgcttatc gctggcagag taatttgcag cgaagtaaat
    61981 aagccatccg cttcttcgcg cagcaacaaa tccgatcatt taaaacaatc atagcatatg
    62041 aagaaacttt caatgataac ggattttgtt ccggccgggg accagcctca ggcgattgat
    62101 cgtctggtgc aaggtctgga aaagggcaat gaacatcagg tccttctcgg ggtaaccggt
    62161 tccggcaaga cgtttacgat tgccaatgtc atcgaacgcg ttcagcgtcc ggcgctgatt
    62221 attgcgcaca ataaaacact ggcggcgcag ttgtatgaag aattcaaggg actcttccct
    62281 gaaaacgccg tggaatattt tgtcagttat tatgattact accagccgga agcctacctg
    62341 ccggtaaccg acacgtatat cgaaaaggat tcggccatca acgaggaaat cgataaactg
    62401 cgccatgcgg caacccatgc tcttttgaca cggcgcgatg ttattatcgt cgccagcgtt
    62461 tcctgtattt acggtttggg atcgccggag gcctatctgg gaatgatcgt ccagctggaa
    62521 gcgggcaagg aattctcacg ggatgaactg ctgaaacggc tgattgaaat tcagtatgag
    62581 agaaacgata tcgattttca tcgcgggacg ttccgcgtcc gcggcgatat cgtggagatc
    62641 tttccgcctt atgaagacga acaggcctta cgaatcgaat ttttcggtga tatgatcgaa
    62701 tcgatgacgt tgattgatcc gttgcgaagc aaaaagatag gcgtcatcca taagactgct
    62761 gtttatcccg gaagccatta tgtttcttcc cgtgaaaatc tgcaacgggc gattcatgac
    62821 attcgccggg aactggcact gacgcttgcc gaatacagag accagaataa actgttggag
    62881 acgcaaaggc tggagcagcg caccaacttc gatctggaaa tgatggagga gatggggtat
    62941 tgtcagggta tcgaaaatta ttcccgtcat ttaaccggac gcaaacccgg tcaaccgccg
    63001 ccgacattga tggaatatct gccgcaggat gccctgatta ttattgatga aagccacgcg
    63061 acactgccgc aactggccgg tatgtaccgc ggcgaccgtt cccgcaagga gacgctggtc
    63121 aaatatggat tccgccttcc ctccgcgctg gacaaccgtc cattgcgttt tgacgaatac
    63181 gaagccctgc ccaatcagcg catctatgtt tcggcgacgc cggcgcagta tgaaatggcc
    63241 aaagcgaaag gtatccgtgt cgagcagatc atccgtccga caggattgat ggatccggag
    63301 atcgaagtca aaccggccac gaatcaggtt gatgatcttc tggcggaaat cagagcgcgg
    63361 attgaaaaaa atgaaaaggt gctcgtgacg acgctgacga aacgtatggc ggaaaatctg
    63421 acggattact acgccggtct ggatatcaag gtgcgctatc ttcactccga tgtacacacg
    63481 ttggagcggg tggacattat ccgcgatctt cgtcggggcg tttttgacgt cctgatcggc
    63541 gtcaacctgc tgcgggaagg attggacatt cccgaagtgt cgttggtggc cattcttgat
    63601 gcagacaaag agggattcct gcgatcggaa cgttcactca tccagaccag cggccgcgcc
    63661 gcgcgcaatg tttccgggaa agtcatcatg tatgctgata agatgaccca ctcgattcaa
    63721 gcctgtttgg atgagacaaa acggcggcgc gccattcagc agaaatacaa tgaagaaaat
    63781 aacatcaccc ctgagaccat taaaaaatcg atcagcagtg ttctgggatc gatttacgaa
    63841 gccgattatg taacgttacc tgcaaccgtg tccgaaagag tcgctccgct aaaggaagaa
    63901 gacctcccgg ctttcattgc aaaactgacc agagaaatga agcaggccgc aaaaaatatg
    63961 gaattcgagc acgccgccga actgcgggac gaaatcagag agctgaacaa aatattgctg
    64021 gaaatgggtt agttctactt tccttcaata tatggtataa atatttattg ccgatgtatg
    64081 ttgagaacat tcgattctgg gaattacggc aggatgaatg aatcggtcac aaaaaagggg
    64141 tgaagcatga aagcactaaa atcgatatta tgcgtgttga cgttatttgt ttttgtattt
    64201 gccggttgcg aaaaagaggc gtccgattgg gttcagtacc ataaagatgc cgacgggaat
    64261 gtctattggt atgatcagaa cagggtaaaa gtggacgcgg caaattatac ggttgaggtc
    64321 tgggcaaaac aagtctattc cgatatgggc agaacgatag aactgcagtc cagaatcaag
    64381 gatggactgt ccatgaaagg gtatgacaag cttgcgcata aaacatgccg gtatgcgatc
    64441 gattgcggga aaaaaggtat aacggtattg tccatcagcc attttgacag cgacggagag
    64501 tcgttatatt tcgatgtcga taaaggtgaa aagaaaatgt ttattatcaa ccctggctct
    64561 ccagcggcgg cccttcaaaa agaagtttgc tcgaagaaac agtgatcctg ttgcgagccg
    64621 ggggcagaaa tgatcattgg taattgtccg ggcgcagaga ttgaaatgaa tgtctcaaga
    64681 aattattgaa gaggaatgct tgtgaataaa gtagtggcac gattggcaaa tggaaaaatg
    64741 atcaagggaa caaccgcaga tttttttccg ggaaaagacg tttttcacgt aaccgaatcg
    64801 gcggccgcgc cgggaaccaa ggcgttggaa gtgttcacga aggatatgaa agcgttattc
    64861 tttgtgaaag atctggcagg caatccgcag cacgtcaaaa ccaacgagtt taatgcatcg
    64921 caaccggttg tcgggcgcaa gatcaaagtg gttttcaagg acggggaagt tctgatcggc
    64981 accacgacag gctatcaacc cggacgtccg gggttcttcc ttgtgccggc ggatgccgga
    65041 tccaataatg aacgttgtta tgtcgtgacg gcgtcggcca aggaaattag ttttctgtaa
    65101 gataagccgc tgaagggccg ggccgtttga ggagggtctc aaagggccaa aattattcaa
    65161 tggaaaggta aaaatcagat gccgcagaaa cagaaaaaga agaaaagaga tatcgaaaaa
    65221 gattatgccg ctgggcagtt tgtcatgaaa ctgagacggc tggctgactg tatcgaaacc
    65281 ggtcatccat tccgtataca gattgcagga gaaaaaatta caattccaac cgatgccgtc
    65341 atcaacatcg aacatgaacg tggcacctca gaagaggaag tggaatttca gattaaatgg
    65401 aagctgaacc ggtaagcatc aaaaatatct ttcccatcat gatcttgata aaaaaacggc
    65461 ttcgatgaaa atgatttaat gggatgtttt gaaagacggg agacacacac catcatgctg
    65521 gacgatatct atcatggata cctggactgg aacatgatgt ttgctgcgga aagactcggc
    65581 cgtcttgatg cacaaaaaac agggattgcg gcttatcttg cccgggacag ttattacaac
    65641 gatccgcgtt atgcggaaac gctgctgaca cttggtttct tcgatgtcca gttattttac
    65701 cacaaccatt tacaggcgct gatcggatgc cgcggtattc agaccccgga aggagccaaa
    65761 gcgctggttg tcattgtgtt cagagggtcg gaccaggtcc aggactggct ccagaatctg
    65821 gatattcgaa tgacgcgttt cctgaataaa gaaaaatttc ctcatttcag ccgcctgaag
    65881 gttcacaatg gaatttttga aagggttcac gattttgaag actggagcag acgcgttatt
    65941 ttcaaggacg gtccgttgaa aggcaacctg cttcagatgc tgggagatga acttaaaaga
    66001 aagcaatgtt tcttctggat tatcggacat agcctgggcg gagccatggc aacgctttat
    66061 gccgcgcgat tatgcgactg ttacaatgtt tcccggaaca gaattctttc ccatacattt
    66121 ggcgctccgc ccattggcaa ccggtatttt gcggcacgct atggacatga atcttgtctt
    66181 cgggcccaaa aaatcaaaag gaagagcgat tctctcgcgt tattccgttt tgtcaatacc
    66241 gaagatccca taccggcacc gagatttgcc gatgtcagag ttgagaaggc tcaaggcgga
    66301 ccgattgcac aaccgccgta tcgattgctg gggtttaaac atgtcggcaa ggaagtgaga
    66361 ttttcaccgc gccacctgcc gggcttttat gaaaaatata agcagttcac gggaaggcgg
    66421 tataacagct cccactttat gaaagttcat tttatggaag cttacatcac cggaatcgat
    66481 gtgttgaata cgcactgagt caattgcgca tgacggaaaa tgtgccggag tgagttgaag
    66541 agtgttttac cggttgaaga ggcggtcatt attcagaaat caattgacac gtaaaagtaa
    66601 cttccttata ttcaaagaag acgttaatca atgatcaacg ggacacaggc gaatcatcgt
    66661 aagcggttta aagtaaaatg accatcaacc attcaacaag gaggacatta tgccgtttga
    66721 aatgatgaaa aaagttatgt tgacaggaat cggccttgct ctgaaaaccc gctcggaaat
    66781 ggaaaccgtg gccaaagaca tcatcaaaaa aagtaagatg agcgaagcag aaggcagaaa
    66841 atttgtagcc gatttgacaa agaaatacga gcagtccaga cgtgacatgg aaaaaacaat
    66901 tcaaaagggc atcgccgact acatggccag cgcggatatt gccagcagga aagaactcaa
    66961 tgcccttaag aaagagatcg gcaatttgaa aaaagcccgc aagaaataag tccgggtatc
    67021 ctggatctgt tgcccggcgc aaacggcttc agaacgtgca agaggatttc agcggaatgt
    67081 taagcatcaa aaaaataggt gtgctgggtc ggacgtaccg caatttcagc cgctatcgac
    67141 aaatcctctc cattcttttc agatatggat ttgatgatct ggtggaaaga ctgaaaatcg
    67201 atcagtatat cgagatcggt cttcagatga tttcccgtca tcagcgcgaa aatgttgaaa
    67261 gattaagccg tccggaacgg gtgcggctgc tttttgaaga actcggtccg acattcatca
    67321 aattcgccca gattctttcc acgcgtccgg accttgttcc tgcggatgtt atccaggaat
    67381 tcgaaaaact ccaggataac gtgccgcctt tccccttctc cgaggcgaaa gcaattatcg
    67441 aatccgagct cggcggtgat ctggataatt ttttcttgtc gtttgaggag aagcctttgg
    67501 cttccgcctc catcgcgcag gtgcataagg ccgtgctcaa tgacggcgag gttgttgccg
    67561 ttaaaatcca gcggccggga ctcaaaaaaa tcatcgaagt cgatctagag atcatgctcc
    67621 atctggcgtc gctgatggag aaaaacatcg aagaaatatc atttcagaag cccacccgtg
    67681 tcgtcgaaga atttgcccga acattggaaa gagagctgga ttttaccact gaagcttcca
    67741 gcatggaacg tgttgccgga cagttcctca gcgacagaac catctacatt cccaaagtct
    67801 tttcagaact gtccggcagg cgggttctca ccatggaata tgtggacggc atcaaggtgt
    67861 cggatatgga aaaacttgaa aaggccggca tggatcttaa aaaaatcacc tcccgcggcg
    67921 ctcatttcgt tataaaacag gtttttgaat tcgggttttt ccatgccgat ccgcatccgg
    67981 gtaacgtctt tgtcctgccg aacaacgtca tttgtgttct cgatttcggc atgaccggtt
    68041 ccgtggacaa acggcaacgc gcactcttcg tggatattat ggaaacgctc ggcagaaaag
    68101 atgcggaaca atgtgcgcgg ctgatgctgg agcttggaga atacgatgat gaaccggata
    68161 tccgcctgct ggaaagagag attgatgatt ttatgggcag gcatcttttc aaatcgctca
    68221 aagacattga tctggcccgt ttgatccagg atttgctcga tattgccacc agcaataaaa
    68281 tcagaatccc cccggttata tttttaatga tgaaagcctt cgccgcgatg gagggcatcg
    68341 cccgcctgct cgatccggaa ttcgatatga ttgcctacgc cgagcctttt attaaacggg
    68401 cgaaactggc ccgatatgcg ccggaaaaaa tcgccgcgga tttattgacc gttgtgtccg
    68461 aatccatgca tatgattcag gcccttcccc gggaagtgtt gcaggtaacc agactgattc
    68521 gtcagaacaa aatgaccatc aatatggaaa tgcgatggat cgataccttt ttgcgcgatc
    68581 ttgatcaggc cggcaaccgt ctgtcctttt ccattatcat tgccgccctg atcatcggtt
    68641 ccgcgtatct tctcgcctac agcaccccgc cgctggtgta tggaatatcc ctcatcgggt
    68701 tgatcgggtt ttccgcagcc gctgttctgg gcggctggct tctgatcgcc atcctgaaaa
    68761 aagggatgct ctgaacaaga tcaaggcggt gttgaatcaa caccgccttg tgtcattgct
    68821 tcttgatttt tcgaacccat cagtggtgca caagcagaac aggggtcttg gttgcctcca
    68881 tgaccttctc ggcaatctga cccatgagcc tcttcaagag tcctgatctg ccgtgcgacg
    68941 gcatcacgat taagtccaca cctttttctt cctgttcttt cagaatttct gccaccggtt
    69001 ctccctctct gatatcactg ataatttcga tctcctggga ttcagggaat ttctgcagct
    69061 cttgttgcat catatccctt gctttaatca ttgtgctttc ttcggctttc ttgaacgtgt
    69121 ccacatccag acaataatcg gctgtgcatt gtgttaccgg atttgcgacg tgaagcagat
    69181 aaactttcga gtggtattgt tttgcgagtt ccaacgcctg tttcaaggct tcatcggagt
    69241 aatcggagaa atctgtcggt acgagaattc tttttggtgc aaacatgacc atccctcctt
    69301 tcacgtatga tcaccaaaac agataagtga ctcacaccaa ctgctttttt ttgcgctgat
    69361 cttatcacct cctttgactg aagattgtaa ttatattata ggcatcccgc atgaaaaaaa
    69421 caatagatct ctcttgctgt gtcatgtggc aaacagcgga catgccgatt tgtagatgga
    69481 gaaaatacag ggtatcattg ataaaataac atgacttgat aaccggcgct aattatgtaa
    69541 aataagaaaa aagtgtccgt gaagattggt catgcctgga ttggggtcaa aaggccgtca
    69601 ttcttataac cggtgtaaat gcttttcgtc tttaatgtga tgcaaatgaa aaactataaa
    69661 atcttgatta tcggttccgg tccggcaggg ctgtttgctg cgcttcggct ggaaaaactc
    69721 ggaatggacg acatagccgt catcgatcgc aatccatatc ccgccggcgg acttctcaac
    69781 gacggcaaac taaacttcga ttaccgcgtc ggtctggaca tcgatgaact gcaaatcgac
    69841 cgtgtaacgg cggagggtat gatgctggaa atcaagcgta tatttacggg atttccgcgc
    69901 tgccgtcagg ttacctttac cgataacaga aataatcttc gggctctggc ggatatggcc
    69961 gccgaacacg gtgcacagtt tattgcgccc gaacagtggc actggggaac ggataacggc
    70021 agggctctgg tggattatct gcggggacag ctgaaaaaaa ccgattttct gctgagcaca
    70081 gaggtcgagt ccgtcgaaaa aaaacgggag ggctccttcc gactgctctg ccgcatcgat
    70141 aaaaaacgaa agagctgttg cgcgccggtc attctggccg ctccgggccg cagcggcgcc
    70201 tactggttcc gcgatatcgc cagagcactg gacatccgga ataatttcgg tccgatcgat
    70261 gtggggatcc gcctggagct caacagaaaa ttctacgact ttgtgaccgg tatcgtttac
    70321 gatccaaagt ttatttttac gacgggacgc cacggcgata aggtcaggac gttttgtacc
    70381 aatcccggcg ggcgggtccg ggtggaaagc tacggtgatt ttaaactggt caatggcgat
    70441 gccctgtcag ggaagaaaac aaagaatacc aattttgccc tgatcaacac cgttgccctg
    70501 accaaacctt attcggatac tacggaattc ggattgagta ttgccaaaca atttttcctg
    70561 ctcggaggcg gcaagccgat cgtccagcgc atcggggatt tccgcgaagg caggagaagt
    70621 tcgctgtcca cgttcaacag cacggcgcgg cattttgaag tctgcagggc gacctgcagc
    70681 gcaacccccg gcgacatcac cctggccatg cccgcacgca tcatggacaa tctgtgggaa
    70741 tcgctcaaat cgctggacaa aatcatcccg ggcattttgc atccgtcaac gttaatgtat
    70801 gcgccggaaa taaaattttt cgatacgcat ttcccgaccg accattatct ggaaacaaac
    70861 gtcaaaggca tctttgtcgc aggggacggt accggcaaaa gccgcggtat tgtgggagcg
    70921 gccatgtccg gcatgatcgc cgccgagggc atcatgaatg gccgatgacg ggtataacga
    70981 tgaaaatacg cggggcgacg ttttatgatt atgatacgag tgccggttga gcggatttgc
    71041 aggaaatcgg gaggataaaa catggataaa gtcgcaaatg aaatcggcca ttatattatc
    71101 cgggcgttcg atgccatggg agcggaaatt atcggagatg tgtttagatg gagcggcgtt
    71161 acggaaacag gctatggcgc aatttttatc gtgctggcca ttctcgtcgt gatttatatt
    71221 gtcaagaaat aaacaagagg aaagatttcc caaaaagcgt caaggattca tgaacaaaac
    71281 ggccatcaga agattcttct tccctgacat aaccgctcgc tatgtgctgc gccttgccgc
    71341 cgtggctgcg gtagctttta ttctgtttaa atatgttttc attccttttc gtattcaggg
    71401 cgagagcatg gcgccgacct atgttaccgg atcgttcaat ttctgttttg ccctgcgtta
    71461 tatgtttgcc tcaccggcac cgccggatgt tgtgacggtg cggatggcgg gacaaaggat
    71521 catgcttttg aagcgcgttg tggcggtttc ggggcagcgc gttgaattca gcaaagggat
    71581 tcttttcgtg gacgacaaaa aaattaatga accctacgtc aagaaaaaaa gtaactggaa
    71641 ccttgaacct attatcgtca aaccggatca cgtgtatgtt gtgggagaca atcgcgcaat
    71701 gcctgctgaa gcgcatacct tcggacaggc gccggtcagc cgtatcgtgg gagtgccgtt
    71761 atggtaaaaa gaaaatatct ggcaatggga ttgattcttg tttcggcttt tgcggtgtat
    71821 ttcttatttt ttgttgattg ggaggcccga gcggttaaaa aacatttgca ggcactggcg
    71881 aaagaactgg aatggtcaac aggggaaaac gaactggcgg tggcgcatcg catcagacgc
    71941 attcaggaag ggatggccga aaactgtcag gttgatattc cgagctacgg aatttccagg
    72001 gctgttgtcc gacatgatgt tccggtatac ctgatgacgg cccgaggcta ttacaaaaat
    72061 ctttcggtcg aactcgagga tctggaaatt gaaacgtttg agcttccggc agcgcaagcc
    72121 gttgccacag cgtatgtaaa agcgattcag gccgatggag tcctaaatga cgaagtgctg
    72181 gtgatccgat tccgtcttca gaaggtcaat aaaaaatggc aggtcaacga agcaggagaa
    72241 gttcatgttc tggaaaaata ataagggtat ttcattttta ctcaaaaaaa atcagagcaa
    72301 caggtcatcg cgaaagacag ggaattatgc aaaaacgaaa tgatcggcag ccctacagtc
    72361 ttgaaagcat tgttgaactt tatccccggc tgtattcgca aaaacgtttg gatgaatgga
    72421 tggatttgtt tgataaacgg gcgatcatgg tgcgggtgca aaagggaaac cctgtttcat
    72481 ttgtcagcat aaccgaaggt ataacggagc aacgcgaata tgccgctgaa aatgcattat
    72541 ttgatgaaaa atggcatcat gtaaaaatta acagacacgg caatattgcc gtcatcaaag
    72601 ccaattacat cctgacggca gaaagagaaa ttcgcaaagg tattgatgtt ctgacattat
    72661 gctgtcgtga cggcggttgg cggattattt gtctggccta tgagcaaaaa gagatggtta
    72721 ggcgttcgat ccctagaaaa gattcggtga aaacatcgta gaaaactggt atgggataca
    72781 gtgtttccat gagggcatta acgttttgaa aggatgaaat gtgcgtaaaa aaattcttca
    72841 gaatcggaaa tatttttatg gaaaaaatat cgttttcggg ccacgtgggc cggctgatga
    72901 gatcgaatga tgatccaccg gattgtgaaa gggagtagtc ataatgaatg aagaaattga
    72961 tgatgactta atcgacattg agctggaagg cgatgccctg gaaaagggaa tgcgaaaact
    73021 ggaaagcgaa gcggaacgat tgctgcatta taccaatgac ctgctgcagg agcttaataa
    73081 ggatcgagca aagcgggaag ccatgaaaca ggaagatgag aaaacgcaaa gtaccgaaga
    73141 aggttctgcc tcgtcataaa gaaaatgtgt ggcgctccta ttctgccggc agaaccccgg
    73201 cattgttttt ttaaagaatt gattgaagaa tgtgtcgccg gaaggggacc ggcaatagcg
    73261 cggatgaagt gagtctggga acataattta aacggctgaa aaaacaaaca gcgagaagga
    73321 gtcattgtct cgtgtgacag tgtagcggtg aaccaacccg aatctttccc gctttttcag
    73381 ttttccggaa catatttccg aaattattta taggacacag acgtcaggcg gactattgca
    73441 tggaagggtc tttttacccg gcaacgctat gaaactttcc cttttgcgca gagcaggcga
    73501 cgaccttatg tatcttgtcg aatccggaga ggacatccta tgctaatggc gatgaaagtt
    73561 ctgaataatc gtaattttat ttttattctt gcgatgatct tgggtctttt tttgccgcag
    73621 ccggcggaat ggaccaaagt tttgatgatt cccgccctgg cattggtgat gacgctggca
    73681 acggtcaatg ttcccaataa ttactttctg aatccgcgtg ccatactgaa accatccctt
    73741 gccggaatgt ttatgagtta tatcattctg gccggtgtcc tgttgtgttt gtcggctttg
    73801 ctcattgaag ataaaaatct gtggattggc tttgttctta ttgcgtccgt gccgcccgcg
    73861 gtggcagtga ttccgttcac ggcaatactg gaaggtaatg ttgattacac actgtccggc
    73921 accgttgcat cttacattgc cgccttgctg atcatgccgg tcattttatg gatttttatc
    73981 gggaccgtgt ttgaagatcc ctataaactt ctgcaaatta tgctcctgct cattgttttg
    74041 ccgctgatac tgtcccggct gattctctat ttcaattggc aggagaaact ctcttctgtt
    74101 cgtggttttc tgaccgactg gggttttttt attgttttat attccatgat cggcgtaaac
    74161 cgtgatttga ttttcagtaa tcctttaacg gtcttttccg tagcggtcat ttctttttgt
    74221 acaacgtttg ttttaggatg gttcattcaa agaatttgtg ctttttgcag aatcgacaaa
    74281 aaaaatatgg tcagtcttct tcttttggga acgctgaaaa atcagggcat tgcgggagga
    74341 ctggccatca cgctttttga gaaggaagcg gccattccct ccgcaatata cagcattttt
    74401 atgattgttt atattatgtg gctggattgg cgaaaatgaa aaacgttcca caaatttaga
    74461 aggtggccga tcagatattc taccggtcga ttcttttttg tggtcaagga ggatttgaat
    74521 ttgacacggg tcggtgtaca attgtaacaa tcatttggca cataaatatg aaaaagggat
    74581 ttgcctgtaa tgcaatagag gaggaacgct tacgtcgcac aatgtttgac tcttttcctg
    74641 aaaaaacaaa aaccgcaaga ttgccgtggg gcagattgaa atgttcagaa atatcgtata
    74701 cgttttattg atgataatat tttccggctc tcttgccatg ggtgaaacgt ctacagctgc
    74761 tgactggttt aacagagcgg atggtttatg ggacgggaaa aaatttattg aagtgaaaga
    74821 agccatggca tatctcgaca aggccattga acaacagccg aattatgcaa acgcttacat
    74881 cagcagaggc atggcgtaca atcagacggg cgactatcaa cgtgcaatac aggattataa
    74941 tctggcgttg ctttttacaa acgatgaaaa ttctttgacg aaaatatttg acaagatggg
    75001 cgtggcgtac gccaatcttg ggcaatatca gcgagccatc aataacttta accatgccat
    75061 cagtctgaag cctgattatg ccgatgcgta caataaccgg ggcattgttt attttcttca
    75121 gggcgacaaa aagcagtgct gtaatgatgc gcaaaaagca tgcgaactga aaaactgcga
    75181 attattgtca tgggccaggg agcggggcgc ctgcccctga tgaaaatggc cgatatccgg
    75241 aatcgttatg cacagggcct gttttttttc actccggttt ctgtgatgtt atgtgataat
    75301 aacagggtgg cggaaaattt tattttatat aggttgataa attattatat ttgtattata
    75361 agaaatcatc ctcgccgaat ccccccaagg tgtctaagct atgcatccca tcctgtatgt
    75421 atagtggcga ggataccttt ttttcaatga gagctttttt tactaccctt tgtctttgaa
    75481 aacaaaccca tatcatcaga aaaggcatat cctaccaatt tgctttgttt tacaacttcg
    75541 acgttttaaa aaatgagaaa ctgaacattt gccgacaagt taagagattc ttacctgata
    75601 gtcatttgtg gttacgatgg gtccggtttt gtatccgaaa tgggtacaat atgcccaccc
    75661 atgatctgta aaatgatgtt aggaaaatag cgataaaact aattagttta attattacaa
    75721 tcgcttacat tttatggcac gggctttgct gttcaagagg gtaactttaa aaaaaggaga
    75781 caagtgatga agaaaataac atttacaatg atcgccgtat ttctggggtt gcttttgacg
    75841 tctcaggcat ttgcgtgggg gccgggtccg gggaaaaagt ccggatattg tcggggagtc
    75901 gttttggaag aattgaatgt gactgaagat cagaaaacaa aacttgatgc cttgcagttg
    75961 cagcatttta aggcggtgca ccccctgcgg gaaaagatac gcgacaaatc tgctgcattg
    76021 cgtaagttat ggctgcaggt cgatccggat aaaaacaaga ttatggccaa acaaaaagaa
    76081 gtaagggtgc tgcgggatca actggaggat aataaaaccg cttaccgctt tgaggtgaac
    76141 aaaattttga cacccaaaca gaaggaaaaa ctggccaaat gcggatggaa caaaaaaagc
    76201 ggattcggtc cgcgcggcgg gaaaaggggt gagcaatgga atggtcatgg gaattgcctc
    76261 tacttaccgt aatgagccgg atgttttttt cattccggaa atatggatga tgcaacatgt
    76321 tattagaata aacaaaaagg agaacataaa atgaaaaata aggtttacaa ggctctgttg
    76381 ctgacgctga ttgtattgat gccggtttcg gcaatggccg atgtaagggt ccatgtgaat
    76441 attcctttgc ctctaccggt tgtctttccg tcgccgccac atctggtggt cataccggac
    76501 actgatgtct atgccgtagc cgacattgga gaagacatat tttttcacgc gggctggtgg
    76561 tggcgtcctt ggaataaccg ctggtaccga tcaagatact acgacagaga ttgggcttat
    76621 catccctatc agccgtcttt ccagagatat gttcctgccg attggcgaca gaattacagg
    76681 cataaaacgt ggaatggata tcgctgggaa ccccagcgga ggtcatatca ggacatccaa
    76741 cgcaattggc atgtctggga gaaaaaccgg cactgggata aacaaagcta tggagttaaa
    76801 aaaataacaa agagagcccc tgcgccgcaa tacagaagca acacgcgacc ggccatgacg
    76861 aagcccgcga tgcagcataa cgtcaaaagc cgaggtgatt tcaggacgtc cgaaagaaga
    76921 gacagacgtt aacgagtcaa ccgctaagac gtgggatata cgaccagcct tgattttcaa
    76981 agatcgaggc tggtcttttt ttcagtaagc acttccggga ttgtcgccag ccgaattcat
    77041 cacttctttc tttccgtctt ttatgttacg taaagcacca taatagaaaa tagttcggga
    77101 aagcttcagc atttatgggc agatcggttt taattttcag aaaccaggag gcatactgtg
    77161 gaagacataa aaattacacc ggctgatgtt caggaaacgc tttgttttac cgggaacatt
    77221 atagaggaat ccggtgcgcg tctcgcaggg agtgaatcct gtaaaaaagc ggctgttctc
    77281 attatgaatg aaatgaataa gcactgcgat tccgtaagca tggaggaatt tgatattcac
    77341 cccaaagcgt ttctggggtt tttcaaggtt gtcgtcgtga tctacatcct gtcctcgttt
    77401 ttgctgtatt ttgattatgt cgttgccggt gcggccggct accttttggg tgctttcatc
    77461 atgttgggcg aatcaatatt ttactgggaa atgctggatc ctttttacag gaaaatgaaa
    77521 gggtacaacg ttatcggaac catagaaccg gaaggcgagg tcaaacaaca gatcatcctg
    77581 agcgggcatc acgacagcgc gcatgaattc aggtttctcg cacatcatca gaaattatat
    77641 gccgtccgga tcatgacggc cgtcataatc atttttgccg cattggttct ctcctggatt
    77701 tgggtgtatc accggtccta tagcggcgcc gatcccgcct tcgtccccta tttacgctac
    77761 ggtgtttttg ccggtctgat ttttgttgcc ccgatgtatt tctttataag tgagaagggc
    77821 actccgggag ccggtgataa catgattgca gtcgcggcga ttattaaact tgcggaaatt
    77881 ttcaacaggg caaggcaaaa cggtgcgggg cttctgaaaa atacccgtct gatgtttcta
    77941 agcgccgatg cggaagaggc cggtcttcga ggcgcgcggg cttttgtaaa acggcatcga
    78001 ggcgccttgc tgaagatgcc gacgtatcat ttcaatatcg acagcatcta caacctcggt
    78061 gaactccagt ttctcactag agatattaac agcgtcgttc gtctgtctaa aaacatggcc
    78121 cgggaatgca aggcagtggc tgatcaactc ggataccgga cgcggctctt tgcaataacg
    78181 cccggaggcg gcgccacgga tgcggcggaa tttgccaagg caggcgtaga agcgacaacg
    78241 ctcatcggta tgccgacgaa tttaatccgg gacggaatgg tctatcacac tcttcaggac
    78301 aacgttgagc atatcgagcc ggaagctgtt gaagccacac ttaaaatcgc gttcgtctat
    78361 attctgaaca aggagcggga taatatttag atcagctgct ggatttttca gaaaagccgt
    78421 gctaaactta atataaccag tatcatagat gtaaattatt taaccattca accttccgtg
    78481 aagttgtcgt gcactgatta ataaatcctg ttgtgatgtc cggtttattt tttatcaggg
    78541 aggaacaggt ttatgacaaa aaatgaaatc agcagggagg agatcattaa aaaactggta
    78601 gattctgatt tggatggctt gtcgcaggaa aagagcatca aatttctggt gcgtattctt
    78661 cataacggct ggaaagggta caatgaaatg tctgatacgg aactgctgga ggcgttcaat
    78721 cacagagaat tcaggaaaac agtcagaacg tgatggatat caaaacggta tataaaattt
    78781 gatcatttgt gtgaacaggc cgatgttgat gcaaggtata ttgtttcacc gttctgacgg
    78841 tattggggaa atagcgtcat cgtggtcttt ctgaataaca atcggatgat acgtttcaat
    78901 atccaaaact gaaagtatcc ggaattttat tctttgacac aaaaccttta tttatatacc
    78961 gttttcatga tgaatgaata aaggtctgaa cccgacattg atcggcgcca tttccacatg
    79021 taaggaagac aggaaaaaat ggaacagcaa aacagacagt caagtgaaaa cgaagaacaa
    79081 tacggatccc ttctggaagc cattgaagag ggatactatg aagtggatct tgccggcaac
    79141 tttacattct tcaacaaggc gatgtgccgg attctgggat atgctccgga tgaaatgcgg
    79201 ggaatgaata accgtcaatt taccgacaaa gaaaatgcaa aaaaactttt tgagaccttc
    79261 aacagggtct ataaaacggg agaatcgacc aaagcgttcg attggcagct catcaggaaa
    79321 gacggaaata aaaaatatat tgaaacctcc gtatccttga tcaaggatgc ttcggggaaa
    79381 ccaacaggtt tcaaaggcat tgttcatgat atcaccgaac gtaagcaggc ggaagaggca
    79441 ctgagagaaa gtgaagcgct ctaccggctg ttggcggaca acatctcgga acatgtctgg
    79501 gttatggatt tgaacctcaa ccttatctac atcagtcctt ccgtggagaa aatatacgga
    79561 tatgctctgg atgaaatcag ccagatcaaa atgaggcaac tttttacggc agactcctac
    79621 aggaaagtcg tggaaacatt catgaaagaa ttggagaaag cgatgaacag caagcctcct
    79681 ccaaccggca cgaactacat catggaactg gaagcctatc acaaggacgg ccataaaata
    79741 tggatcgaga accgcctgca ttttatccgc gataaaaatg ggaaaccggt atctcttttg
    79801 ggagaaaccc gggatatcac cgaccgcaaa cttgctcaag aagctctgaa ggcgagtgag
    79861 gaaaaatacc gtcttctggc cgatcacatg aaagatcagg tctggatgat ggatatgaac
    79921 atgcagatta cctatgtcag cccgtccgtg gaaagaatca cgggatattc gtttgaagaa
    79981 ataaaaatat tgcctttgga tcaactgctt actcccgatt cggtcagaaa ggccacggag
    80041 tttattacca acaggatgcc gaaagccttt aatctggttt ctctggattc tcaatataga
    80101 acactggaac tggagttccg gacaaaagaa ggtaaaacga tatgggggga atgttcattt
    80161 aattttatac gcaatgaaaa taataaaccg gtttccattt tgggtgaagc gagaaatatc
    80221 acggatcgca aaatagccga agataaactg cgtcagagtg aagagaagta caggacgatt
    80281 ctggaggaca ttaaggaagg ctattttgaa accgacctct tcggaaactt tacgttcttt
    80341 aatgatactg tgtgccgggt ccttgggtac tccagaaacg aattgacggg catgagtaat
    80401 aaaatctaca cggacgcgga agaacggaaa aaagtatatc gcgccttcca tcaggtttat
    80461 atgaccggag aaccttacag ggatctggag tggcaaatcc gtggaaaaga cggcacgagc
    80521 aagtatattg aaggatttat atcccttttg agagattctt cgggtaaccc gaaaggattc
    80581 agaggcattg cccacgatat aacggagaga aaaatagccg atgaaaagct gcaaaaaaca
    80641 ctggagagcc tgaaaaaggc cgtcggtacc accatacagg tgctcatttc cgccctggaa
    80701 gcgcgggacc cctacacggc aggccatcag tcccgcacgg caaatctgg
//
LOCUS       DAONQZ010000004        77334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_326_length_77334_cov_13.808085, whole genome shotgun sequence.
ACCESSION   DAONQZ010000004 DAONQZ010000000
VERSION     DAONQZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 77334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77334
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_77334_cov_13.808085"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLR13_01445"
     CDS             complement(<1..272)
                     /locus_tag="PLR13_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQC18020.1"
                     /translation="MDEEQIKQEMIDYLMKKITGGLSDKVVDYGNNPRHCGTLTKPDG
                     YAKITGPCGDTMEMFLRIRNDKIEDIAYTTDGCMTSHAAGSAATVMA"
     gene            complement(338..736)
                     /locus_tag="PLR13_01450"
     CDS             complement(338..736)
                     /locus_tag="PLR13_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQC18021.1"
                     /translation="MVNVTEEIKESLKAAKIAYLATAAKDGTPNVVPIGAFKFMDDET
                     LLISDQYFSKTLNNLKSNPKIALSWWAEKGGFQIKADITIHTDDDIFRQNVEWVHGIK
                     DTLKPKSAIVGKITAVYAVRSGPDAGKQIL"
     gene            1059..1622
                     /locus_tag="PLR13_01455"
     CDS             1059..1622
                     /locus_tag="PLR13_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18022.1"
                     /translation="MSNVEKVKGTMLIDQVRMIRGNKDKDWRNYLTSEDWAIVKGMVM
                     PASWYPVETYKRCGRAVFQVLAGGNTDIVRLRGKMRGKELFETTYKHLLMERYPMNAL
                     SSFVNLYGQLFMPNPLGFKKMTDQHAHVSYEYEDKNDPGNLPFCYQLMGHFDSLIEMT
                     GGADVKIEFAKKMWEGASETVFDIQWQ"
     gene            1674..2234
                     /locus_tag="PLR13_01460"
     CDS             1674..2234
                     /locus_tag="PLR13_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18023.1"
                     /translation="MNVKGIIFNTGKSTIIKVFGQGPWDAFMTKLVAKDKFFGNVIMS
                     VTPIPLDKFIFFLDELVKEFFNNDMMQYVTFGKVAAQFALSPDGLYKSYLLTKDTKQF
                     VESVMPKFWSTYFDEGTVTTKYENNITHLKITGLNFRHNYFEYLIMGFFQKSLKMFGK
                     KNVPKRIRSRASGDDDVYFQFELKDS"
     gene            complement(2412..3257)
                     /gene="ccsB"
                     /locus_tag="PLR13_01465"
     CDS             complement(2412..3257)
                     /gene="ccsB"
                     /locus_tag="PLR13_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HQC18024.1"
                     /translation="MNHTLILSYVTFIYFASFVFYLFRMILKKEFWGYAASLLAWLGL
                     FAHTLAMMLRWRASYDLGFGHVPITNLYESLVFFAWSIIMLYLAIEWRIKSRILGTFV
                     VPVAFFVMAYASIAPGVDHSIEPLIPALQSNWLTTHVLTCFMGYAAFTVAFGCGLLYL
                     WTEKKETRETNPASHPKENSSVLNTLDVLIYQSTSLGFIFLAMGIITGSVWAHYAWGS
                     YWSWDPKEIWSLITWLIYAIMLHARFVRGWRGRRMAMMTIVGFVSVLFTYLGVNFLTS
                     LHNYI"
     gene            complement(3254..4591)
                     /locus_tag="PLR13_01470"
     CDS             complement(3254..4591)
                     /locus_tag="PLR13_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HQC18025.1"
                     /translation="MKKANFIWSFLASVKLAIVLLFFMVVIFIVATLVPPQRMDSEWL
                     WLNDLYHSKMFYVLMGLFSLNLMVCSIQRIPFAIKQYKTPSFPPPSGLYENLAPNRII
                     VTDKKMETVSRAVESLLKSKLFSVKETKLEKGKMLYRESGRLSLFGVYVVHLGVLMII
                     AGMVIGSVFGLEADLRLSEGEETNIIQLRNGEGEHPLDFSVRCDKFVVEFYDTGVPKT
                     YRSELSFIKNGQVVRQGSVLVNHPLSFDGLRFYQASYGVSEKSRARLTYSNAGAESPE
                     ISAGQGDTFDLPVQKAKATVLRVEENMMQFGPAVKLNIETDKRNIQFWVFQHIQDIAD
                     VNPGLFSAVPLFNPGLFKPLVFSLRGIEGKYYTGLQVVRDPGVPFVLAGGLILIAGMM
                     VIFFIAYRRIWFLLEQDPAGVRIRVAGRSHRYNEALQRRLDDLCAWMEKELAA"
     gene            4783..6048
                     /gene="nrfD"
                     /locus_tag="PLR13_01475"
     CDS             4783..6048
                     /gene="nrfD"
                     /locus_tag="PLR13_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQC18026.1"
                     /translation="MADSGKKRFLPHIKSAFERVLSGGWIYLLFVGLFGIFLLIGIGA
                     GIHALFIAGSRHAYATSREIPVAILIATYVFFVVASTGLCLVSSLGHVFGVENFLPLA
                     KRCVYLAIITILSGFLVIGLEIENPFRMAIYNVISPNFSSNIWWMGTLYGFAVVFLTI
                     EFILLFNEAHHHKAVIAGFLGVITEVAANSNLGGVFAMMHGRPYWYGPYFPVYFILSA
                     LITGCAFILFFFFLAHKAVNKKPFMESTQTALKAVSKLTLLLLAVMMIFTAWKVLTTS
                     VGSPAAAAALSVLTEGPYSFNFWTIEILVGMVIPFVLILASRGRNLTVMFIAAVMMIF
                     SLFFTRLDMVVVGQIVPQYFEVGVVEYSKLHTYSPSIHEILVVLGGISFCLLAFLLGE
                     KVFSGFADSPQTDIMKEKTTDVAGVEELT"
     gene            6045..6782
                     /locus_tag="PLR13_01480"
     CDS             6045..6782
                     /locus_tag="PLR13_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC18027.1"
                     /translation="MTDKASHKNIPESPFDAERRAFLKIGLKITGVLAGGSILSLVSN
                     INKAYSLDLFREKYPYKPHYAMVLRQDRCIDCEKCMKACIETNHVPDYGWRTKILKRD
                     LSSALDQKWEFIPILCNQCNNPPCVRTCPTTATYKDKTNGIVQMNNKKCIGCKSCIMA
                     CPYDARYYNEEKGAIDKCDFCFESRLSKGINTPACVEACPSDVRIFGDLSDPSSRVFQ
                     LVHQLQRPVWVLRQEIGAKPNIFYMKG"
     gene            6793..7545
                     /locus_tag="PLR13_01485"
     CDS             6793..7545
                     /locus_tag="PLR13_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQC18028.1"
                     /translation="MKRNLVLIILLALFCIANVGSTGKSSFSDGGGILYTQPVKSVIF
                     RHQHHVDVKKISCEKCHSGLFEMQALLAQEKKDFIMDSLYKGRYCGACHNGKDAFASD
                     KQCARCHVRISEKDVGHVKGKAKPYKAPVYSTVATLGKDQRKVPFNHEKHASLNCMDC
                     HSKLFQIRKGSTKIALETHQSQKYCFNCHNGKKEFSWNNCTKCHQNWKDIKPYGILTA
                     RADRGSCVACHTNPDEMKSLVKPPEIGGEAEG"
     gene            7609..8604
                     /locus_tag="PLR13_01490"
     CDS             7609..8604
                     /locus_tag="PLR13_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18029.1"
                     /translation="MIGKEFHLSMDCIACHQGNKLAKTKEKAHQGRIARPSDNLKTCG
                     ICHITAGQNYAKALHYTSLGQREGVKHRFSAAELKTFDQKVFEKSCRSCHASCGSCHV
                     KSPPVGGITTGLLQGHKFVKKNEGKTCASCHGGRVYPEFTGEYGGTPDVHYQKGMMCN
                     DCHNKQEMHGDGKLYKSKNDVKDRPKCLSCHQDKGNQVAHTIHKDKVSCQGCHSSGQY
                     RQCYSCHAVHGSKAEPGFILGLNPRDRKTLTTLRVIPTIRSTFEPNGIKMENFDALPN
                     YWNSPVHNIKKRTNRTRSCDACHVNKEGFLKPEGLIKDGSKENEKLIYDIKPISK"
     gene            8621..9547
                     /locus_tag="PLR13_01495"
     CDS             8621..9547
                     /locus_tag="PLR13_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQC18030.1"
                     /translation="MKRMRFLKVLLVSLLILLPAAVLAQGLSPIVSTDDLAKIVKDPN
                     VIVIDIRKVEDYKAGHIPGAIGVFYNNWAPGMGGLKNEMPADDDLMDLLTDNGIQPDS
                     TVVVYGTTGTPPDRVDVTRVAWALKYAGVQKVSVLSGGFEKWAVREKKEVSTEITKPK
                     TKDYKGKINKAILVNKDYVKNHVGKAMIVDAREPAFFKGEQKLPFVAKAGRVKNSVNL
                     PTIQVFDKYPPGEHMEPCCWTYKDVATLKNIASGVVGSDIDKEIIVYCDTGKVASAWW
                     FILSDVMGFKNVKIYDGSMEEWMNDASAPTEP"
     gene            complement(9626..10549)
                     /locus_tag="PLR13_01500"
     CDS             complement(9626..10549)
                     /locus_tag="PLR13_01500"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQC18031.1"
                     /translation="MKNHALSKLLTILLFILLLPIAGQTRDIEPIVSTDWLLANLKNP
                     KLIILDVRRVEDYREGHIPGALNAFYRSWAYQKDGLFTEIPETDDLDDTIGYFGIGFD
                     SRIVVTGCMDTPRLSYQSARVACTLQYAGIKNVAVLNGGMNKWITEKKPLSQEIMHAK
                     AKPFKGLYSRARFADKDYVKERLGKAIMLDVREREFFTGEKKMDCIPMMGRIPGAFNM
                     PTSCAFNPDKTFKTTEELAAISEAAIGRDKEKEIITYCDIGQCCPIWSYILVEVLGYK
                     NVRLYDGAMQEWMQDPQAPITKNADANVTPK"
     gene            complement(10683..11228)
                     /locus_tag="PLR13_01505"
     CDS             complement(10683..11228)
                     /locus_tag="PLR13_01505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(11010..11012),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQC18032.1"
                     /translation="MRITKFVFFVLVFFILSACASTGAKNASPVSGQVAPDFTLSDQK
                     GNIWKLSNAVKNHRAVVLAFYPKDDTKLUIRQFVEFQQNIKKYKAKKIKILGLSRDSA
                     ESHRKFIAKHSLKNMTLLVDEKGKIAKLYDADHWLLPLSKRVYVIVDKKMNIIYRNDM
                     GFALLPDQTQTLMGEIDRHIR"
     gene            complement(11297..>11881)
                     /gene="prxU"
                     /locus_tag="PLR13_01510"
     CDS             complement(11297..>11881)
                     /gene="prxU"
                     /locus_tag="PLR13_01510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081651649.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(11768..11770),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HQC18033.1"
                     /translation="DFTAPAYHKGKFINVKLSDYLGKWVVLCFYPGDFTFVUATEISS
                     VGEKYPEFKKLGVEVLSMSVDSVFVHKMWDDHELSKMVKGGIPFPMLSDGGGKVGDVF
                     GVYLQDAGVEARGRFIIDPDGIIQGFEVLTPPVGRNVMESIRQIQAFQHVRKTKGAEA
                     TPSGWKPGKPTLKPGPDLVGKVWEVWKTKMAFDD"
     assembly_gap    11882..11981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11982..13594)
                     /locus_tag="PLR13_01515"
     CDS             complement(<11982..13594)
                     /locus_tag="PLR13_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC18034.1"
                     /translation="MAQRKRIIVIGGSAAGPKAAAKARRIDNDAEVIIIQKEADLSMA
                     SCGYPYYVGGFFNDRNALLCTPAGVVRNPMFYLNAKDIEAKVNTEVTNIDTKNRSVSF
                     KNTVSGETGTLTYDKLVIATGAVPRMPPVPGTDLQGITTLQSMKDADFLRNVRDEGKI
                     KKAVVIGGGLIGIETCEALQLAGIEITVIELLPQLLTFLDWELAKLVENHVRSKGANI
                     ITENGIAAFLGENGKLTAVKLQNGTELPCELAVIAIGVRPNVTLAKEAGLKIGDLGGI
                     EVNEYMQTSDPDIYAVGDCVETLNRITGKKVHAPYGDLANLQGRIAGENAASGNSVTF
                     PGTIQTGICKVFDYGAGSTGLSETMAKRLGYSDIITVINASPDKPGFMEGKLLVTKLV
                     AEQKTGRILGAQCIGPGNVAKQIAQWAMAIQGKMTVEDLINADLPYAPPFSLAIDHFI
                     ATAHIMQNKMKGRMKGISCKEVYDKIRAGEKPFLIDTRGPEEFEEMRLGIGETLIPLG
                     AIRKRLKELPEDKNREIILYCKISLRGYEA"
     gene            complement(13844..15037)
                     /locus_tag="PLR13_01520"
     CDS             complement(13844..15037)
                     /locus_tag="PLR13_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQC18035.1"
                     /translation="MSYAKLSDWNKCTTCGECLVQCPVMHMDKPEAVAEMTGLLKTGY
                     SEKIFRECTFCFSCNNYCPEGLRLHEFILEKFMENRKKKGKISAVYPYLFNGMKMSMW
                     KDIYAMLSREEQSILKKWSEIPPPSKEILWVGCIGRISCFDIENSAVLQELPKFGPPD
                     LCCGELAYRLGSWKAYTETIEKTLKAFEKLNIERMVCYCGSCYNYLSNILKNVYGREL
                     PFKLISLYEWLNEKRESGKITVKQPLKLKTTLHESCYVSELGDGFARDLRAVYKSAGA
                     DIVELPHHGECNLSCGAVSVLRTLNLPKSLFKEQWKKYKEVKETGMKDMAVNCPGCFI
                     TLSFTNRIAGIKLHYMPDELLKAYGDTITKPLSRRIPLFIRAFIKHPTLPLRHVDAEI
                     SPASS"
     gene            complement(15057..15791)
                     /locus_tag="PLR13_01525"
     CDS             complement(15057..15791)
                     /locus_tag="PLR13_01525"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQC18036.1"
                     /translation="MNKTIWKPEPEINLILKDYKTFGILSCGTCANLSYTGGKTGVEV
                     LKAHLLKNHKQIKLDKVILAACPEEIMRQALRANKKALEKCDALIVLSCAAGIKSANS
                     CRPDLPVINVLDSVGSAAVSSSDPVIAHSLCRSCGHCVLTCTAGICPLSECPAKSKYG
                     PCKSYSESNLKCALDPQINCVWHIIHERSNNLHLLPLLKELHSHKEDRNTHSTHEIKS
                     AGLWQKFFAWSAARIQKLEWVIRQFR"
     gene            complement(15847..16329)
                     /locus_tag="PLR13_01530"
     CDS             complement(15847..16329)
                     /locus_tag="PLR13_01530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQC18037.1"
                     /translation="MKQKAFQDYYPDDLSHCYGCGRLNEHGLQIKSYWDGEESVCVYQ
                     PDPRYMAIPGMAYGGLIASLIDCHSTGTAAAAKYCSENREMDTLPPIRFLTASLHVNY
                     LLPTPLGGPIEIRGKVKEIKRRKVVIESRLIAGGQICATGEVVAIQVPEALMPDHLKE
                     "
     gene            complement(16363..16539)
                     /locus_tag="PLR13_01535"
     CDS             complement(16363..16539)
                     /locus_tag="PLR13_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18038.1"
                     /translation="MKTKFETILDVKIYTIDAVEALPYNFRSSTNVIFDNEHVHVDIA
                     TDAQKMHAFLSSRL"
     gene            complement(16634..17584)
                     /locus_tag="PLR13_01540"
     CDS             complement(16634..17584)
                     /locus_tag="PLR13_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18039.1"
                     /translation="MTTALKAVDRVEILTLQDNYIEITAMDSNAVVTRANPLKEGEIR
                     ASVLAEHGFSALVQTTLGNKTHTLLLDFGFSENGAAQNAETLNVDMTQVEAAALSHGH
                     SDHTGGMAKLVTMTGKKNIPFAVHPSAFKSPRYLKFGEELKINFPELTREMVKAAGLT
                     VVETEKPYLLLEDTILFLGEIPRSTDFETGWPLPHCQQDGKEVWDAIEDDTSIVMNLK
                     NKGLIILSGCAHSGIINTVHHAMKVTGINKIHAVMGGFHLSGPFFEPIIDRTTQEFKK
                     INPAYIIPTHCTGRKAIMEMEKQMPEQFILNMAGTKLTFN"
     gene            complement(17605..17943)
                     /locus_tag="PLR13_01545"
     CDS             complement(17605..17943)
                     /locus_tag="PLR13_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQC18040.1"
                     /translation="MKIAIPLAEGKLTAHFGHCASFAIIEIEDQTKKIIKREDIAAPP
                     HEPGLLPPWLAKQGANMIIAGGMGQRAQQLFAEQGIQVVVGAPVETPEKLITDFFAGS
                     LQVGDNLCDH"
     gene            complement(17956..18867)
                     /locus_tag="PLR13_01550"
     CDS             complement(17956..18867)
                     /locus_tag="PLR13_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQC18041.1"
                     /translation="MEDIIVEEKKTCDTCGDSDCAAKKQKKDESPEEYADRRKLQATL
                     CRIQKKIVVLSGKGGVGKSTVAVNLATALALNGLRVGLLDVDIHGPSVPTMLGLENET
                     LKGGPDGLFPVEFGGLKIMSLGFLLQNPDDAVIWRGPMKMGVIKQFLTDVHWGDLDYL
                     IVDAPPGTGDEPLSLCQLIQPLDGAIIVTTPQKVAAVDVRKSISFCKRLDVPVVGVIE
                     NMSGFACPKCGEITQILPTGGGKKISTDMNVPFLGAIPMDPAIAQSGDSGKVFIYHSS
                     KTPTGEIMQKIIDEITKDLDKTKILNS"
     gene            complement(18873..19256)
                     /locus_tag="PLR13_01555"
     CDS             complement(18873..19256)
                     /locus_tag="PLR13_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQC18042.1"
                     /translation="MSELVNLDETYATAKKHADSPHNMGPMDSFSGHARITGPCGDTM
                     EFWILVEEGKIRKVSFITDGCDPSRACGSMTTCMAEGKSIEEAQAISQKDVLNVLDGL
                     PEDHEHCALLATNTLKAACEDYLNQ"
     gene            complement(19268..20149)
                     /locus_tag="PLR13_01560"
     CDS             complement(19268..20149)
                     /locus_tag="PLR13_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18043.1"
                     /translation="MKELVIISGKGGTGKTSVTASLVALAKRSVICDCDVDAADLHLV
                     LTPQIKEEHEFISGHEAVIRQTDCISCGICKDVCRFNAIHSKQNFQGELLFAVDPVAC
                     EGCKVCVEMCPEKAIDFPDCNCGKWFVSDTRLGPMVHARLGVAAENSGKLVSTVRREA
                     RAIAEKENCELIIVDGPPGIGCPVIASVTGASQVLVVTEPTVSGEHDLERVLGLTRHF
                     QIPAAVCVNKWDINPDMTERIEENAAGLGARIAGRIRYDNAVTQAQIQKKSVVEIDAP
                     SGDDIQQLWKNLNLREV"
     gene            complement(20155..20784)
                     /locus_tag="PLR13_01565"
     CDS             complement(20155..20784)
                     /locus_tag="PLR13_01565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18044.1"
                     /translation="MQKDIIENHIKYQKRVALYRTFGYNIDLERSFIIEKSLPVAGNI
                     LEAGTGKGYFTLALAQAGYKFFSLDISVAEQRFALLNLMYYGFQSQVTFLIADVESIP
                     CNDGFFDVIFAVNMIHHLSSIRKVCDEFTRLLSPDGKIVLSDFNEKGLAILDRIHERE
                     GRYHDVSGGTLAEMKTILVERGFTVREHQSDLQDMLVAGRTNLSPDIEE"
     gene            complement(20800..21750)
                     /locus_tag="PLR13_01570"
     CDS             complement(20800..21750)
                     /locus_tag="PLR13_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18045.1"
                     /translation="MSERVYQYVYGPVPSRRLGKSLGVDIVPYKTCTYDCIYCQLGKT
                     TNKTTVRLDYVPLADVLSEIEEKLLTKFECNYITVAGSGEPTLHASIGEIIGKIKERT
                     NIPVAVLTNGSLLYLPEVRQSLMKADIVIPSLDAGNEQLFQYVNRPHKDISFERVIQG
                     LIDFSKQFTGHIWLEILLLSGINGMAEEVKEMAAWVNKIHIEKVQINTVSRPAYEDFA
                     HPVDVRQLQKLARLFLCPVDILQNATSSDLSGINGVESSDKDILDLLARRPCTLEGIS
                     TGMGLHPHDVAKKLKSLLDKRLIIAMRAEQGLFYKIAERK"
     gene            complement(21752..22009)
                     /locus_tag="PLR13_01575"
     CDS             complement(21752..22009)
                     /locus_tag="PLR13_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQC18046.1"
                     /translation="MPTYEYECSKCGLRFERQQSMKDAPIRICPECRADVQRIVSGGS
                     GFIMKSAGVSMRDTAKSCSFEETGTTCCGAKQRCGQAPCGD"
     gene            complement(22016..22858)
                     /locus_tag="PLR13_01580"
     CDS             complement(22016..22858)
                     /locus_tag="PLR13_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18047.1"
                     /translation="MKLAVASGKGGTGKTTVSVNLARVFKRDVCLADCDVEEPNAHLF
                     LKTSGHSEKTIGLPVPDVNEELCNGCGECAKICQFSAIVTFGTTPLVFPEMCHGCGGC
                     TLVCPTKAISEKNRRIGVVETSRTENITLVTGKLNVGVALAPPLIRAVKNTLPVDAPA
                     ILDAPPGTSCPVIAAIRDSDFVLLVTEPTPFGLHDLSLAVDMVRELNLPFGVLVNRMG
                     SGDNRVHEFCRKENVPILMEIPDDRQIAENYSRGILMVDALPQYRERFENLLEKIANS
                     KKKG"
     gene            complement(22855..23469)
                     /locus_tag="PLR13_01585"
     CDS             complement(22855..23469)
                     /locus_tag="PLR13_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18048.1"
                     /translation="MKAGMIAQELKLHAPFTMFGTVTGIVIMAAFIQFQVPREFSSGL
                     FWTMHPLHVLLSALVTTAMYRMHSRGNILQIIIIGYIGSIGIATFSDSFIPYIGEWLL
                     ELPHRGVHLGFIEKWWLVNPLALAGIALGFVISHTRIPHAGHVLLSTWASLFHVLMAL
                     GTSVDVVTTILIAFFLFLAVWVPCCTSDIVFPLLWINPKESKIL"
     gene            complement(23609..24100)
                     /gene="tsaA"
                     /locus_tag="PLR13_01590"
     CDS             complement(23609..24100)
                     /gene="tsaA"
                     /locus_tag="PLR13_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HQC18049.1"
                     /translation="MINRQIIYHPIGIIHSEHTVAEKTPIQPVYACGCKGRIEVFEKY
                     ISGLSDLDGFSHVYLIYHFHQAPLVQLKVKPFLQDVERGIFSTRAPGRPNPLGLSVVE
                     LIRIEDNILYVDGIDILDGTPLLDIKPYTAKFDLHDIRKNGWQDEVDESTAAKRGKRG
                     YRS"
     gene            complement(24097..24459)
                     /locus_tag="PLR13_01595"
     CDS             complement(24097..24459)
                     /locus_tag="PLR13_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQC18050.1"
                     /translation="MKIALTTSGVDLNAPLDSRFGRAPKFLIYDQAANTFEIVDNEQN
                     LNAAQGAGIQSAQNIARLGVKALITGHCGPKAFRTLQAAGIRIYNTSAATVSEALEQY
                     REGTLTEAKSADVDGHWV"
     gene            complement(24576..24950)
                     /locus_tag="PLR13_01600"
     CDS             complement(24576..24950)
                     /locus_tag="PLR13_01600"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HQC18051.1"
                     /translation="MPRGDATGPIGMGPMTGRGAGYCAGFGAPGYMNHIPARRFGAGF
                     GRGAGFGGRGGRGGGFGFGNRFYAAAIPGWRRTDEYSAAVVPPDPHEEQQALRTQAEF
                     LQRELDAIKKRLDELKTDTQNN"
     gene            complement(24973..25212)
                     /locus_tag="PLR13_01605"
     CDS             complement(24973..25212)
                     /locus_tag="PLR13_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18052.1"
                     /translation="MNTNNRNPRGTGGGMSQGSGSGMGRGRGGGGRGRMGGMKAGGAL
                     GNCLCPKCGNRQPHDRGIPCTQVKCPQCGSTMIRE"
     gene            complement(25242..>25602)
                     /locus_tag="PLR13_01610"
     CDS             complement(25242..>25602)
                     /locus_tag="PLR13_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HQC18053.1"
                     /translation="PGAITFCVMSRCCSNEPRRLISASVGCAIPMDKKTYGYISEHHS
                     FGYNEKQTGDYAEDLAAAMLASTLGIDFNIDESWDEKKELFKISGKIVGTRNTTQSTV
                     LKTKDYTTVLAAAVFVF"
     assembly_gap    25603..25702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25703..26058)
                     /locus_tag="PLR13_01615"
     CDS             complement(<25703..26058)
                     /locus_tag="PLR13_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQC18054.1"
                     /translation="MKLALSVFKDCISTVFDSAEQLLIVEADTANPDKRTLQKFVATD
                     PAGRAAELKNRGVDVLICGAISRPMQALIMAQGIIVHPFVRGEVHAIITAYRNNTLGL
                     PVFSLPGCRRRAMGAG"
     gene            complement(26232..27602)
                     /locus_tag="PLR13_01620"
     CDS             complement(26232..27602)
                     /locus_tag="PLR13_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQC18055.1"
                     /translation="MKKKMPLKQGSPDIGTKQDIILDSIADGVFTVDQDWLITSFNRA
                     AEKITGVPREEAIGQRCKDVLKADICEKDCCLRKTMKTGEQIINKKVAIINAEGHKLP
                     VSISTALLRDQKGKLLGAVETFRDISVEEDLRKAVLQKYNFADILSKNHKMLQLFDIL
                     PDIAAGGSTVLIEGESGTGKELIARAIHNLSPRRKTSFVAVNCSALPDTLLESELFGY
                     KAGAFTDAKKDKPGRFKLAENGTLLLDEIGEITPAMQVKLLRVLQEKTYEPLGATQSV
                     EHNVRVIAATNKNLEDLVRQGRFREDLFYRINVFKITLPPLRERMEDIPLLIDHFISR
                     FDLLQKKEIEGVSQEALSVLMAYTYPGNIRELANIIERAFIFCKEGRIEKKHLPESLF
                     ASPPQKIRTDASSLRDVEAAWLINALQKNNWNCRETARQLGIHKSTLYRKIKALGITL
                     PGQFPL"
     gene            complement(27631..27987)
                     /locus_tag="PLR13_01625"
     CDS             complement(27631..27987)
                     /locus_tag="PLR13_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQC18056.1"
                     /translation="MKIALPSYQNQIDEHFGHCEYFTVFTIDQEKKIVEEEKVSSPAG
                     CGCKSNIAEVLAAKGVKLMLAGNMGQGAVNVLNSHGIDVLRGCSGNVKTVTESWLAGT
                     LKDSGISCTQHEHGCH"
     gene            complement(28048..28341)
                     /locus_tag="PLR13_01630"
     CDS             complement(28048..28341)
                     /locus_tag="PLR13_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HQC18057.1"
                     /translation="MPRPKCHRHIMGAPDKKYFKPRGIPSMELEEIVLNMDEFEAVRL
                     ADYEHLYQKQAAEKMNISRQTFGRIIDVAHKKIADVLINGKALRIGGDFDFDE"
     gene            28598..29386
                     /locus_tag="PLR13_01635"
     CDS             28598..29386
                     /locus_tag="PLR13_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQC18058.1"
                     /translation="MQKHISNVHVHMPYSLLPKYLKIILQNKLNLEIYFHHQILNRLD
                     KSRCMETAKILESEGLKITFHAPFVDLRPGALDETIRRASIDRIRQVFDLAPYFHPLK
                     IVCHASFDDRYYVYGDDLWLESSLKTWKEFIGLADDHRTIIALENVYEKNPVILGKLF
                     RSLASDRICFCFDTGHFNVFSCEPLNVWFDELGRYTGHLHLHDNFGWQDEHLSVGHGT
                     FPFAELMQYIRNMKKKPTVTLEAHNQKNLWASLDHVKRMDFPGV"
     gene            29417..30391
                     /locus_tag="PLR13_01640"
     CDS             29417..30391
                     /locus_tag="PLR13_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18059.1"
                     /translation="MLRYVLKRTLFMIPLLIGITLICFFVMRLAPGSPTDLQTEMNPK
                     ASQESKERLNALYELDKPIYVQYGSWLKKISRGDLGVSFSSDHRPVADKIMERLPITI
                     LINFLSLVIILAVAVPIGVLSAVHQDSLFDKVTTVLVFIGFAVPTFWLALLLMIFFGI
                     HLGWLPISGLRSLNYEYLNVWNQLIDLAKHLVLPVFVSAFGGLAGLSRYMRANMLEVI
                     RQDYIMTARAKGLSERDVIYRHALHNALLPAITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTIMGILLIGAILTLVGNLIADVSYAVADPRIRIS"
     gene            30405..31232
                     /locus_tag="PLR13_01645"
     CDS             30405..31232
                     /locus_tag="PLR13_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18060.1"
                     /translation="MTAWTAIYKNKLMLTGGGLVLALLIVSILAPWLSPYDPGQIDLN
                     NVLAAPSMEHLFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILVGTFLGALSGYYG
                     GWIDSVIMRFVDIMLCFPTFFLILAVIALLEPSIWNIMIIIGLTGWMGVTRLVRADFI
                     SLRERDFVQAARAIGAGDARIIFLHILPNAMASILVAATLGIAGAILTESALSFLGIG
                     VQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILTTVVGYNLLGEGIRDLLDPRLKK"
     gene            31326..31493
                     /locus_tag="PLR13_01650"
     CDS             31326..31493
                     /locus_tag="PLR13_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18061.1"
                     /translation="MPNRVNAESISDDKRILKRKLMQGEISEKDIQALIKKLPDVSEN
                     AEEVRLDDDKK"
     gene            31500..32279
                     /locus_tag="PLR13_01655"
     CDS             31500..32279
                     /locus_tag="PLR13_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQC18062.1"
                     /translation="MLIDSHAHLEMKEFDADRDDVISRARSEGVDFIVTVGTNLRLSS
                     RAVALAEQYENIFATVGIHPHDVAKAGEDTLDALKQLVLTHKKIVAYGEIGLDYFRNI
                     SRQEIQVEMFGQQLDLVKELNLPVVIHDREAHRQSLDMIQASGVRRGVFHCFSGDYDM
                     ARKCIDLGFYISIPGVVTFDKSGKIRDVVERVPLSSLLLETDAPYLTPVPNRGKRNEP
                     AFIVHTAKAVAAIKKISWEDVAAQTSVNTINLFGLKMIRNV"
     gene            32300..32995
                     /gene="queC"
                     /locus_tag="PLR13_01660"
     CDS             32300..32995
                     /gene="queC"
                     /locus_tag="PLR13_01660"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQC18063.1"
                     /translation="MKKQFKAVVLLSGGLDSATTLAIARDRGFEIYALSFRYGQRHAV
                     EMKAARRIARKNKVAGHLILNMDLRKIGGSALTADIHVPKNQTGSVLKRKIPVTYVPA
                     RNTIFLAYALAWAEVIGSHDIFIGVNALDYSGYPDCRPRYIAAFRKLANLATKAGVEG
                     KQKLAIHAPLIKMSKAEIIKKGMDLGVDYSLTHSCYDPSPSGEPCGECDSCLLRLKGF
                     REAGLKDPLKYCR"
     gene            33006..33647
                     /gene="queE"
                     /locus_tag="PLR13_01665"
     CDS             33006..33647
                     /gene="queE"
                     /locus_tag="PLR13_01665"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HQC18064.1"
                     /translation="MGYRIKEIFYSIQGEGFYTGRPAVLCRFSGCNLWSGKASDREKA
                     VCRFCDTDFVGCDGPEGGIFQTPEALTEKIITLWPEKPSDTLRPFVVLTGGEPLLQTD
                     RALLDALHHQHVEVAVETNGTIMPPEGIDWICVSPKAGTTMVLRSGQELKLVYPQQGA
                     EPEKYALLDFQHYFLQPLDSPNRQTNTLRALEYVLAHPQWRLSLQIQKILNVK"
     gene            33659..34018
                     /gene="queD"
                     /locus_tag="PLR13_01670"
     CDS             33659..34018
                     /gene="queD"
                     /locus_tag="PLR13_01670"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQC18065.1"
                     /translation="MEIFKVYKFDAAHRLPKVGAEHKCFELHGHSFRVEIHVQGPVDP
                     QLDWVMDFADIDRAFRPLLDQLDHQNLNDIPGLNNPISENIARWIWENLQDALPQLNK
                     IVVQESPESGCIYCGEK"
     gene            34177..34578
                     /locus_tag="PLR13_01675"
     CDS             34177..34578
                     /locus_tag="PLR13_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18066.1"
                     /translation="MDQKAIQKIKGTQQDGLLEELLREKAAVLTRASWAVEDVLEKMK
                     RIEGEIEIKTSLIQSMTHEADRAVLSRQKKLFCEEINCKIDEFNAIRRKAQLQYYYLI
                     VTREALGLRRHEMVQKIYRIPDKKKKIQVFF"
     gene            34578..35231
                     /locus_tag="PLR13_01680"
     CDS             34578..35231
                     /locus_tag="PLR13_01680"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQC18067.1"
                     /translation="MDRFRKPRMRMVETQIKARGISDVHVLKAMQTIPRHLFVDEGLI
                     EQAYTDSPLPIGEHQTISQPYIVALMTQALDLKGRERVLEIGTGSGYQTAVLACLADR
                     VFSVERIALLATQARRTLDALNIYNVAIRVGDGSYGWKEEAPFDAVITTAAVPAIPRY
                     LLDQLVPGGRMIVPSGGRDVQTLYKLTRSKENPEEIIQEDLGGCRFVSLIGESGWRK"
     assembly_gap    35256..35355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35356..35841
                     /locus_tag="PLR13_01685"
     CDS             <35356..35841
                     /locus_tag="PLR13_01685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQC18068.1"
                     /translation="TGDLQDSATAKIRDDKPTDPKNKTVEEMVVPNRKIKATHNKFLW
                     PVQGEIKAHFGIQPNKTFHNWVKIVSASGKKIKAAESGTVIFSSHLKNYGETIIIRHK
                     DHFATVYTHLKKRLVKMDTPVKKGEAIAVLGEKDDDGAVYMNFEVRLKGKAVNPLSYL
                     P"
     gene            35906..37051
                     /locus_tag="PLR13_01690"
     CDS             35906..37051
                     /locus_tag="PLR13_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18069.1"
                     /translation="MKCYFEQGPIRPPSEAASLLIRVSRNCPWNKCAFCHTYRGTKFE
                     LRSVEDVKKDIQNMKEIADEIVRFSWKLGEGGRVNRNVLRAIYNSEDFYHDNIYSVAA
                     WLYFGGENVFLQDANSLILKTPDLVSILDFLKEKFPDVQRITSYCRSHTAARKSIAEF
                     EQLKKAGLSRIHIGMESGSDEVLSFIRKGVTSADHIRAGLNIRGAGIELSEYVIPGLG
                     GERFTRQHALETARVINAINPDFIRLRTLHVVPGTDLDELMNKGEFKPLTDDDILREI
                     KLFIENLDVRGTYLASDHILNLLEELEGSLPDDRQNMLAAIEKYFKLSAEDRLVFRLG
                     RRSGSLRKLDDLADQATYGRLRSVVDHYAARGENLDEDIIRTMNNFI"
     gene            37428..38600
                     /locus_tag="PLR13_01695"
     CDS             37428..38600
                     /locus_tag="PLR13_01695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC18070.1"
                     /translation="MRECVIIDGVRSPNSRAHNEKGWFRALRPDEVLTKVYDGLFARN
                     PKVKPEDIESVFVGCANITGMQNDIGRLAWLAGGFPESVPTNTLTMQCPSGMAATQHA
                     ARAIMCGEGDTYIASGVEDMQKVFMAMHMDFPPRLANRYNILELPMGNTAEKVAEVYK
                     VSRADMETMAYWSNKKADEATKAGKFKNEIIPMEGLKDDGTPFMVETDQWIRGDVSME
                     QMATMKTPFKPDGVVTAATSSPLTIGAAAILLMERSKADKLGLSYSIKYEAGALGGCD
                     PTVMGIGPIPAVQRLLARTGKKVSDIGVWELNEAFASQSLACLRELGIAQNAPFDNVN
                     IWGGALALGHPLGESGCRILVTLHSIMKTDRKDAKYGVASLCGAFGNAGATMLSRV"
     gene            complement(38698..39645)
                     /locus_tag="PLR13_01700"
     CDS             complement(38698..39645)
                     /locus_tag="PLR13_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC18071.1"
                     /translation="MKTLKALIPILLLCIMAVTSGCTALGWNTVPVEKLKLKYADAES
                     EIIEIDEMKIHYKDEGQGPVLILLHGVCASLHTWDGWTQRLKDRYRIIRLDIPGFGLT
                     GPAPDKSLYRKDEAVRLFEKVVDEMKLEKFYLAGNSLGGYISWNYTLKHPDRVEKLIL
                     VDSVGFPQPLPGLLAFASNPLIRPFARFTMPRFLFDNAVEQVFGDKSKVTQELKDRYF
                     DLAMREGNKGSYIDVFTEMRKLCRDENLSQGIKDIRVPTLVMWGTRDEWIPFKYFENW
                     KKELPGAKFIQYEGAGHTPMEEIPDETARDADLFLSGKM"
     gene            39867..40307
                     /locus_tag="PLR13_01705"
     CDS             39867..40307
                     /locus_tag="PLR13_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18072.1"
                     /translation="MKTYAIKYLELAEKHAEKIAARWLKDVKNNSRTPTYKSLNEAEI
                     TNQCVRFYQTFSKMFLDEKITDDSLRYFRSYAEESHTMGIPAKETLYALILMRRHIWL
                     YAEFQAIFTSGIDQRQALDTLGRTILLFDYACYEVTKEYQSLTR"
     gene            40378..40818
                     /locus_tag="PLR13_01710"
     CDS             40378..40818
                     /locus_tag="PLR13_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18073.1"
                     /translation="MVDSIKLLDITEQNAEEIAEHWAKEVHKNKRTTHYRNIKKEKLV
                     VYAVDFYRNLRKLLVPEDRLEYTREYFQKYAKKCHEIGLPLQEAIYGLVLMRRHMWLY
                     ADFQAIFIDALEHNQAIDSIMRIMLMMDYAVYEITQYYLETAKH"
     gene            41215..43056
                     /locus_tag="PLR13_01715"
     CDS             41215..43056
                     /locus_tag="PLR13_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQC18074.1"
                     /translation="MASLWVDLRDIKFQLFEVLKIEETLLGKGRYKDFDADTCIMALD
                     AAAKFSENDIAPTYPDEVHRKPVEATFDKGKVTTPEAYKRLWKLYCENGYMAMADQPE
                     VGGQGFPAVIAAAAANMFLACNQAFVMYPGLSHGCARLMEDFFQHPLRDIVLEKMYTG
                     EWAGTMCLTEPGAGSDVGALKTTAKKNADGTYSIVGTKSFISAGDHDLTPNIIHPVLA
                     RIEGDPKGTKGISIFMVPKIRFNEKGELGEPNDVQCGNIESKMGIHGNATCTLNFGSD
                     NKCIGYLMGEERQGMPIMFHMMNEERQGVGMMGASLAMAAYLHALDYSKQRIQGQDVI
                     MMAMKVEDAPAVPIIKHPDVRRMLLKQKAISEGLRLLAYFCYFCMDNEFSAMIDGNEE
                     QKEFWHGLIEVLTPIVKSYCTEKGQDSIELAVQCYGGYGFCREYPVEQMMRDNKINLI
                     YEGTNGIQALDLLGRKLGMKRGAYFMNLLSVTKEAIEEGKKLDAFKKEAETVEKALNA
                     CAETALAFSKMIKTTPFVPLIGACDFLNCLGDALVGWLHLRMALVAIPKFFAADTGEM
                     DKAFYRGKIEGARFFINRITGLVIPHLENLKQDEQSAMNIPEESFAV"
     assembly_gap    43253..43352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            43549..44049
                     /locus_tag="PLR13_01720"
     CDS             43549..44049
                     /locus_tag="PLR13_01720"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQC18075.1"
                     /translation="MRKRKPQTRLQSISEILFPILKKRGMISKIEEKTLQTLWPKAVG
                     PQIALQTRPDSLRNGTLFVKTASSVWVQQLHFMKEEILVKLNNLSGKPLIKEIRFVVG
                     HSPVNEKEANPVLKEKKAVLKKRDRDMIAECTEALIDQELAAVLKRIMRLEISRRRQW
                     ESRKGR"
     gene            complement(44009..44761)
                     /locus_tag="PLR13_01725"
     CDS             complement(44009..44761)
                     /locus_tag="PLR13_01725"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HQC18076.1"
                     /translation="MEEETLDEILHGALRIFQKKKGYRFSIDSILLAHFVSLKKRTRL
                     MDIGCGSGIILLILTKRFPTTQCVGLEIQERLAVMARKNVQLNQLENRIDIVWGDARN
                     VRTIFQERTFDAVVFNPPYRKLHSGRINPLEERAIARHEIQGSLKDFLNAAKYLLKPA
                     GTVFTIYPAKRLVELVYLFRLHGIEPKKMKLIFSDVSSGAEFVLVEGRKGSKEELKVE
                     PPMFIYDRPGKYSEKMSGVFSDLSGFPIDDAG"
     gene            complement(44783..46024)
                     /locus_tag="PLR13_01730"
     CDS             complement(44783..46024)
                     /locus_tag="PLR13_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQC18077.1"
                     /translation="MAIVLPFKAVRPDKKFVAQVAALPYDVMTREEGKKAVSGNPLSF
                     MHVEKSEIDVPDKTKSDDPLIYQTAKRNFTDMRVKGILIQDESPHFYVYRQKMGTRAQ
                     TGIVGVMNASEYDAGKIKKHELTRQDKEEDRIRHVDTVNAQTGPVFISYRERPGINTI
                     VDHVTFGMPEYDFTSDDGVTHTVWIISDSRHIEEIKKEFLAVDALYIADGHHRAAAAA
                     AIARTRRAKESARGFAGEYESVLAVFFPDTQLRVMDYNRAVKDLNGLTPDQFIDKIGS
                     AFIVSKDFSDRSPNQLHDFGMYLGGQWHKLTVKEGVYDKNDPVASLDAAILQEHLLYP
                     VLGIKDPRVDDRIKFIGGIRGMDELEKLVNQDGFAVAFSLYPTTMEQIINVADAGAIM
                     PPKSTWFEPKLRSGLFTHRLD"
     gene            46125..46601
                     /locus_tag="PLR13_01735"
     CDS             46125..46601
                     /locus_tag="PLR13_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC18078.1"
                     /translation="MDTLVFIHGLESTAQGTKGQFFCKNFPQMIIENYTGDFAARMRK
                     LQSLLNDKTDLIIVGSSYGGLMAVQYAIENEHRVKKLILLAPALNLAEFTPPSRVLNV
                     PVILYHGSEDDIVDPEKVKDIASRYFAALEYHLVPDDHVLHETFPSLDWKKLLTAG"
     gene            46899..47309
                     /locus_tag="PLR13_01740"
     CDS             46899..47309
                     /locus_tag="PLR13_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC18079.1"
                     /translation="MVSKKIVIRYTPDTVQQPVIYQLVKQYNLVFNILKARIFPRREG
                     VIVLELSGSKENFDHGIRFLKELGLKVEPLSKSVSQNADKCVHCGACTAFCPTGALSF
                     EKKTLKVVFDPEKCNGCELCVSACPVRAMEINLL"
     gene            complement(47362..47736)
                     /locus_tag="PLR13_01745"
     CDS             complement(47362..47736)
                     /locus_tag="PLR13_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18080.1"
                     /translation="MDVITGYISTHPAVVVIGAILIILLFLHVIFKNLLKLIFITALL
                     LIVAFGYYYVQHPAETKAKVEESFHMVESGLNEIKDKSKSFYKDGKDLYKKTKEAPGG
                     VSKLLDASEKEVEKEVGKETKK"
     gene            47987..48706
                     /gene="rnc"
                     /locus_tag="PLR13_01750"
     CDS             47987..48706
                     /gene="rnc"
                     /locus_tag="PLR13_01750"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQC18081.1"
                     /translation="MDKSRREVLKKLEERLDYRFRDVDLLVTALTHRSYVNENRQPGV
                     SDNERLEFLGDSVLGLCVSDLLIKKYVDGSEGDLTKMRSVLVGEKNLAQLARGLQIGN
                     CLLIGRGEENAGSRARDSFLANAFEAVVAAVYLDSGYENVRAVVRKFIEPFLEKETLP
                     SAYFDYKTLLQEFCQRKFKTIPMYLLADTSGPEHAKVFEVKVVIVDKLTAIGTGTSKK
                     EAEKQAAQKAWKILQDDESQL"
     gene            48687..49796
                     /locus_tag="PLR13_01755"
     CDS             48687..49796
                     /locus_tag="PLR13_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18082.1"
                     /translation="MTKASYRKPNDNYPLIIPVFIMNSGCPHRCVFCNQKISAGNFPE
                     KITQEYFDRTVTSYLEWNKDKTRRVEIAFYGGSFTGIDSGDQEALLAMADAFIQRGLV
                     HSIRISTRPDYIFEKQLSMLKKYNVATVEIGAESFVDDVLRYAGRGHCAADTEKAVMM
                     LKKYGFKTGLHLMAGLPGDTAEGFDYSLDRTIELSPDTARIHPVLVFAGTALAEEFRK
                     GAYRPLDIASAVELCRRAWEKLAAAGIRIIRMGVQTTPEMEKQGTVLAGPVHPAFGSL
                     VRSSVFYHSTVNVLKKISGDVQEIRFYLSERDLSIFRGLNNINTEAIKKLYPRAKLII
                     ESQSDRPRGELSVAVNSGPLLHWTIPGFHQLVRGQ"
     gene            49802..50692
                     /gene="era"
                     /locus_tag="PLR13_01760"
     CDS             49802..50692
                     /gene="era"
                     /locus_tag="PLR13_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQC18083.1"
                     /translation="MFKSGFIGIIGRPNAGKSTLFNAIIGDKISIMTHKPQTTRNKIT
                     GIKNYPDAQLIFLDTPGMHKPKTPLNRAMVRTTREAIGSVDVLLMMIEAQTDVQPHDL
                     FLIESLNETKAPVLLVINKIDLIEKKFLLPLMDKFRRIYDFRDIVPISALHGNGVEEL
                     LTIVKEMLPEGPKYYPDDIVTDATERFIASEFIREKITLHTKQELPYSAAVEIDLFKE
                     DEAKNLIRITATINVEKDSQKAIMIGKKGAMLKKIGTQARLDMENLFGAKVYLELFVR
                     VRKEWTASERMLEEFGLLKH"
     gene            50700..52019
                     /gene="der"
                     /locus_tag="PLR13_01765"
     CDS             50700..52019
                     /gene="der"
                     /locus_tag="PLR13_01765"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQC18084.1"
                     /translation="MKIKPVIAIVGRPNVGKSTLFNRIAGKQKAIVIDEPGATRDRNY
                     MDCAYHDRAFTLIDTGGFEPASEEKILVQMREQSNLAIEEADVIIFMMDGKEGLTTSD
                     MEIARQLRGKGKKVFYVANKVDGERYKEMLSDFYRLGIDEVYPVSAQHNLGVDELMNA
                     MVSDFPESSEEKSKNDDQIKVAIVGKPNVGKSSLVNKILGKERTIANPIAGTTRDAID
                     STVKIHGKKYLIIDTAGIRRKSKISQTLEKYSVVQALKSINRCDIVLVLIDAEEGITE
                     QDTKITGLAYERGKACLIVVNKWDKIKKDNATVGKYVEEIKDKLKFLDFAPIIFISAV
                     TGQRAPKIFDLIDKVYAQYTQRIGTSPLNNLVEQAVRKNPLPRYQNRLMQIYYATQTD
                     IKPPTFVFFVSNSKGIHFSYERYLMNQIREKFEFDSVPLKLIFRKKS"
     assembly_gap    52550..52649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <52650..53438
                     /locus_tag="PLR13_01770"
     CDS             <52650..53438
                     /locus_tag="PLR13_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC18085.1"
                     /translation="GVRLDISGAVPENARTCLIVWSCMGGAVQMYRVPVEKFTDQGCA
                     CILYNPRGHGRSEGELEIQTALNDLDEILQENVPSAIPLVIVGHSAGANASLQFGVRC
                     LPPKQFVLIAPVLDSRESLFYMYQRKTIDEFVSILCAYVQDDTLVRKVLVDDKWLDAD
                     LWKARHYTHLLDHVHARMRIGTFLERLFIPGHNAFAQLERFSAQTRIFMAGEDTWYPA
                     ATTRRLADKYGIPVETLPEAQNHFFTGAWDSMWTKILNLILLPG"
     gene            53664..55631
                     /locus_tag="PLR13_01775"
     CDS             53664..55631
                     /locus_tag="PLR13_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQC18086.1"
                     /translation="MIYFRNISLSFLDKKLFDKMNWTIHPKSRIGLVGENGTGKTTLF
                     RMIMDDVRPDDGTLDIPGKKQKIFGYLPQDLVELESIPLIDYLKQKSGMADLETSIKD
                     LEQKLSKIKHDTAEYRELSQKYSDATALFHARDGYAFESRAKQMLKGFGFKESDFKQN
                     CDSFSGGWKMRILLTSILLAKPDIMLLDEPTNHLDTESMEWLESYLKDFRGTMIVISH
                     DHFFLDKVTTQIAELSSCAIHLYKGNYTHYLTEKEKRQDALKKEMELQKSQIKKIEEF
                     VERFRYKASKAAQVQSRIKMLEKFSEVTPEGKTKNVAMKFPEGKKSAKEVVTVTGLGH
                     RYGDLTVFGDLNFALFRGDKVAFVGMNGSGKSTLSRLLGKMENPSSGVAKYGDGVNMA
                     FYSQESSQNLNYEKTILEEINAVPTDASDQEKRNLLGTFLFSGDDIFKPVSVLSGGEK
                     SRLALLKIMLLDTNFLILDEPTNHLDIKTKDIFQNALIHYRGTVAIVSHDRYFLDHLV
                     NKVFELKDGAIHYYNGNYSYFIEQRSRPADAAGDEKPAQGVIRNQIVNAKEKKRLEAE
                     ERNKTYKIRAALKKELSDVEKKISGLEDRKTRQETALCDPQTKKDARTIKSINLELKD
                     INRDLEQSYTIWTDLHMKLEQISANAEPFSS"
     gene            complement(55628..56146)
                     /locus_tag="PLR13_01780"
     CDS             complement(55628..56146)
                     /locus_tag="PLR13_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQC18087.1"
                     /translation="MNKRISALKFALDNELHERDFYLANARRTKNLAGKNMFKQIADE
                     EKEHYDMLKKLFEKWEAKEKWPATIPLKVKKSVAGNIIKAMSEKKGARIKGNEDDLKA
                     IRTAIDFEARGMALYTKLEQGSDDPKEKAFFNLLASIERQHFLSLRDTEEFLTDPASW
                     YQNMEKASLDGG"
     gene            complement(56260..57864)
                     /locus_tag="PLR13_01785"
     CDS             complement(56260..57864)
                     /locus_tag="PLR13_01785"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HQC18088.1"
                     /translation="MAIKDIFIIVSGVILFLIGMVNLSSSIRKLIDVRIKKFIKYAVG
                     RPISGLLTGIVSTIAFQSSSASTVLTIGLVSAGLISFSNSLAIILGADIGTTLTVQLV
                     IWRFTDISPLIISLGGLLWLIGRNKWETIGEIIFYFGLMFFGLDLVNKAVEPLKQSPL
                     LIELFTKTQNPLIGIGMGIAVTGIVQASAIPISIMVLLAQQDIVSLENAVAVVMGANI
                     GTTITALLAGTVATRSGKRTAFSHLIFKSTGIGLCFMAMPSFVWILQHISSSVAQQIA
                     LAHFLLNLFIVVIFIFLLSPFAAMMTRFFPGKDDILPVWPEYLDVQDITNPQKALDNV
                     HKELKRQTNMVRDMYLKSMRMINDYSPSDDKSLFYIAMVVKNIREQIVGYLWKLSVQD
                     LSEYYSKNVFAYTAMTSDIESIGNHVLCISKLTAQKTGKNIKFSDCGEVELHEIIDLV
                     SDNFNDAFSVIDKPLDKTIMDVIAREEQVDIKVKEARDNHLERFHKRLCDPEAGPIFV
                     ELLLHLERISDLCNNIAEYMLDVKED"
     gene            complement(58202..59224)
                     /locus_tag="PLR13_01790"
     CDS             complement(58202..59224)
                     /locus_tag="PLR13_01790"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18089.1"
                     /translation="MNKLCYDIPVLLLHNMNPEWTSEETLETEQDVATLGNALKDLGH
                     PLEIVTVRDDRLAQQLHNFNPRDYVVFNWCEELPGVPHSEPDVAAVMERMGFVFTGSS
                     RDVLALAHDKPEIKRVLDDANIPTPPWAVFDNTDVGEWNIFPSIVKAANEHCSIGISP
                     ESVVTTPKELENRVAFILDKYQQPAIVEQFINGREFLNAVWGNGHISMLPAVEMDYSE
                     LTDLRDRLFSYEAKYVAGSRLYESIDMRVPAELETSARAQLENVVIATYRATGCRDYG
                     RIDVRYLNGIFYVIDVNPNPDINPQTSMTHAAAEIGYSYGQMGSRIVNLAAARHPIFS
                     QQYNPA"
     gene            59532..59948
                     /gene="rnk"
                     /locus_tag="PLR13_01795"
     CDS             59532..59948
                     /gene="rnk"
                     /locus_tag="PLR13_01795"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQC18090.1"
                     /translation="MADRTIYITKFDMDRLLELIDGVRSTPRHNKANIDLLEKELYNG
                     TVVDSEDVPADVITMNSRVSVTDIESGEQMTYTLVFPSEANIAENKLSILVPLGMALI
                     GYRKGDIIEWPVPSGVRKIKVNDVIYQPEAAGNYNL"
     gene            complement(60000..61082)
                     /gene="rfbB"
                     /locus_tag="PLR13_01800"
     CDS             complement(60000..61082)
                     /gene="rfbB"
                     /locus_tag="PLR13_01800"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQC18091.1"
                     /translation="MTKNIHPPVTLHNLLVTGGCGFIGSNFIRYLIDRIHHSGRIINV
                     DKLTYAGNPENLAEIAANGASRYIFQKADICDRARLEEIFSRYDIDGVCHFAAESHVD
                     RSIKTPGDFIQTNIVGTFNLLECAKARGDRFKIFHHVSTDEVYGSLGADGLFREDTPY
                     RPNSPYSASKAASDHLVRAYYKTFGLPVTISNCSNNYGPYQFPEKLIPLIVLNALEGK
                     PLPVYGDGKNVRDWLYVEDHCEAIWTIMTAGRHGETYNVGGKAEMENIAIVQLICDIL
                     DDIQPASDNSPRRRLITFVKDRPGHDRRYAIDFSKLQKELNWAPKESFSSGLQKTVNW
                     YLDQKKWVENIKTGAYLNWIKEQYGA"
     gene            complement(61117..62037)
                     /locus_tag="PLR13_01805"
     CDS             complement(61117..62037)
                     /locus_tag="PLR13_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQC18092.1"
                     /translation="MLNALDKKLLKTVADLESLPKGAYNIRKNGKLLHREVSANINIE
                     TNADGTGIIVTIKPGTKNESVHIPVILSQAGLYDVVYNSFIIGEGAEVTIVAGCGIHC
                     GSSEAEGHAGIHDFHVGKKAKVTYVEKHFAMGEGKGRRSLNPTTKVFLDEGATAEMEL
                     TQISGVDEANRLNEAELGPDSLLLIMERVLTEGEQSAVSTNNIILKGFGSRANMISRS
                     VMKGNSKQIFHANMEARNKCFGHIECDAIIMDNGTNETMPALKALHPDAELTHEASIG
                     KIANDQLTKLMSLGLTYDQAINRIIQGFLK"
     gene            complement(62018..62773)
                     /locus_tag="PLR13_01810"
     CDS             complement(62018..62773)
                     /locus_tag="PLR13_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC18093.1"
                     /translation="MLTLNKVFFTAPGGDGENSAPRNIIDGLDFTFEDGKFYAITGPN
                     GSGKTTLAKLIMGINPITQGSIVFNGQDISGLSITDRAKLGIAYSFQQPARFKGISFR
                     DLLSIATGTDEEKKFLEIVSRVGICPLSFLDKEVDSKLSGGEIKKIELATTIATNPKL
                     AIYDEPDTGIDLWTIGPMVSLLKKELTPKTTTIVVSHNKAFLDVADVIIILNQGRIAY
                     TGNLKDALPLLNDMSICAYHNKLEGFENAECFR"
     gene            complement(63104..63253)
                     /locus_tag="PLR13_01815"
     CDS             complement(63104..63253)
                     /locus_tag="PLR13_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18094.1"
                     /translation="MATAKKAAPKKKVAKKAAKKAAPKKKVAKKAVKKAAPKKKVAKK
                     AAKKK"
     gene            complement(63673..64452)
                     /locus_tag="PLR13_01820"
     CDS             complement(63673..64452)
                     /locus_tag="PLR13_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQC18095.1"
                     /translation="MEYRIMKKYRLIICLFLLFTLMLACSGKAHHRRVASAPRYKPAL
                     NLMGYTIQAGAFRNVENAVRLTERLKINHGLDATYFLADDKFFKVRFGNFSTKDLAKK
                     RALELIDAKVIEEFYIVRPEDYSVARQKQYGTDYLRESIVTTARDFLGVPYLWGGASP
                     DDGFDCSGLTMTVYQLNGLNLPRNSRKQYAVGNAIEKNELQKGDLVFFADRGNHHVSH
                     VGIYVGDNQFIHASSQGNEIRIDHLSSDYFTRQYVGARTYL"
     gene            complement(64434..65771)
                     /locus_tag="PLR13_01825"
     CDS             complement(64434..65771)
                     /locus_tag="PLR13_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HQC18096.1"
                     /translation="MPDHAHRLHAREIIHRLRESGHEAYFVGGCVRDFILNQTSSDYD
                     IVTSARPEEVMKLFPRTVAIGAKFGVVAVIIDNHPFEVATFRSDDVYEDGRHPSRIHY
                     STAREDVFRRDFTINGLLMDPDTGDIIDYVNGRFDIDNKIVRTIGNPGKRFREDYLRL
                     LRAVRFAANLHFDLEPATQDAVKREASNIRHISVERIREELNKILTRGGARRGFELMM
                     QTDILQNILPEICRLKGVEQPPRFHPEGDVWQHTLKMLELLPADGEREKNICLAWGTL
                     LHDVGKALTRSEDEKGVHFYGHVQQGEIIADTIMQRLKFSRMQRETVVNLIRQHMVFM
                     NVRKMRPGRLKRFLRMPDFDLHLELHRLDCLASHGMLDNYEFCREQLQILALDDLHPP
                     RLLTGNDLIAMGFTPGKIIKDILLAVEEEQLEGRIQSAEQAINYVRNRWNIES"
     gene            complement(65790..67061)
                     /gene="thiC"
                     /locus_tag="PLR13_01830"
     CDS             complement(65790..67061)
                     /gene="thiC"
                     /locus_tag="PLR13_01830"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HQC18097.1"
                     /translation="MTQLEKARKGIITEAMKAAAKEEKVAPEYIRKGIAEGTIVLCRN
                     VKHPSIKPLAIGRGLRTKVNANIGTSKDHTDLNLELKKLKIAVDAGADAVMDLSTGGN
                     LAAIRKKVMKKSTVAIGTVPIYQAAVKMLQDRKAISEMTADNIFDVIEENGRDGVDFI
                     TVHCGVTRLSVSALKSQKRILGIVSRGGSMTANWMDCNKKENPLYEEYDRLLEIAHRY
                     DMVLSLGDGLRPGAIDDATDQAQLQELIILGALAARARAAGVQVMIEGPGHVPLTDIV
                     TNIRLQKDICQNAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYVTPAEHL
                     RLPTLNDVRDGVIAARIAAHIADIAKGVKQAREKDRKMSSCRKNFDWQGQVDLSIDPE
                     KTVSLLEKSKSAKNEGCTMCGEFCAIKLGKR"
     gene            complement(67104..67904)
                     /gene="thiD"
                     /locus_tag="PLR13_01835"
     CDS             complement(67104..67904)
                     /gene="thiD"
                     /locus_tag="PLR13_01835"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HQC18098.1"
                     /translation="MKLRKVLCIGGSDSSGGAGIQADLKAVSACGCWGLTVVTAVTAQ
                     NTKGVSGIYPVDPAFVAKQMDVVLSDIGVDAIKTGMLPTEEIIQVVVKKIKKYKLKNV
                     VVDPVMIAKGGRTLMTGKARQMLVQTLLPLALVITPNIPEAETLSKNKIKTMADMKDA
                     AAVIYESGAANVVIKGGHLPDRKHADVTDILYSGSGYCEFSSARIRSCHTHGTGCTFA
                     SGLASGLARRKSLADAVLQAKITVADAIRKAVVLGHGYGSVNVFEKFS"
     gene            complement(67907..68578)
                     /locus_tag="PLR13_01840"
     CDS             complement(67907..68578)
                     /locus_tag="PLR13_01840"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQC18099.1"
                     /translation="MRGKVIIVSGGESTDAAFFNSQAGKRDDCLIIGCDGGSRHLKSW
                     GIKPDVIIGDMDSIDAALLAYYDSENIKIIKYPANKDFTDTELALDYALEVKPEKIYI
                     WCALGGRMDHTLSNIFLLFKGRERGIQTVLIDPFCEIFLADNETHFTGDTGKTVSLLS
                     LSPEVTGITLSGFLYPLENENLKMGESRGISNRIRDNDACIRFANGKLLVIKYQNKDR
                     FPEAV"
     gene            68881..69348
                     /locus_tag="PLR13_01845"
     CDS             68881..69348
                     /locus_tag="PLR13_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18100.1"
                     /translation="MRRVSLLLLILTVALLFSACGKDEPELSCIYPGLYEINHDIKYS
                     GQLVILKQRVRYHADGTFEATNFQNNAALEEMKGKYKVENKQLISYDTQRRLITQEGT
                     WTPPKKEPSAVELRKIKKGSYQYYFKYPDEQMREKYKGLGLAEGWKTYKRISD"
     gene            complement(69444..70178)
                     /locus_tag="PLR13_01850"
     CDS             complement(69444..70178)
                     /locus_tag="PLR13_01850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQC18101.1"
                     /translation="MPEQNRENSLVLIKPDAIKNSLTGYILSRFSEFHTGLRFAALKV
                     VAVNLALAEEHYAEHKGKFFYPSLLEYIRGYLHYPDEPSKRRVIAIIYRGPNAIKNIR
                     EICGNTNPHEARAQRPGCIRSLGTIIPLYDAKGKFIGDRSDNLIHASANIQDAEREIK
                     LWFLPTDIPPTVRSYPAETSKEYYYYKNGAISSEYTPGSYCFIGPGDLVWKSDMKVLR
                     QIAKGKKQDYSTNAVIAKYLINKETD"
     gene            complement(70275..71618)
                     /gene="trmFO"
                     /locus_tag="PLR13_01855"
     CDS             complement(70275..71618)
                     /gene="trmFO"
                     /locus_tag="PLR13_01855"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HQC18102.1"
                     /translation="MDHRVTIIGGGLAGCEAAWQLLRRGHAVEMYEMKPQKFSEAHKM
                     ENLAELVCSNSLKSNSLDNAPGLLKEEMRLLDSVIMKAADKTAVPAGSALAVDRLLFA
                     REVEARLSEQAAFTLYRHEVTAIPNDTVTIIATGPLTSDAMAHDISRRLGGASLYFYD
                     AIAPIVEADSIDMNKVFWASRYDKGTPDYLNCPLTREEYESFHKALLAGEKVAARDFE
                     ETRHFESCLPIEILAQRGEKSLAFGPMKPVGLIDPHTGNKPYAVVQLRRENAAGTLFN
                     LVGFQTKLTWPEQRRIFRLIPGLEKVEFARYGSIHRNTYINSPALLSPTLQLKNHENI
                     FFAGQITGVEGYMESAAMGLLAGLNAAFIIEGKKAEPPPVNTALGALIQYITSPEHAV
                     QFQPMNINFGLFNAPVEKKLRKKERNALIVHQALQSLNAWISSQKLNELRPDIFC"
     gene            complement(71618..73960)
                     /gene="topA"
                     /locus_tag="PLR13_01860"
     CDS             complement(71618..73960)
                     /gene="topA"
                     /locus_tag="PLR13_01860"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQC18103.1"
                     /translation="MANSLVIVESPTKVKTIKKFLGNDFNAVASMGHIKDLPKSKLGI
                     DLEKDFEPSYSIIETKKKTIDELKKAAKNAENIYLAPDPDREGEAIAWHINEVLNTKN
                     KNVYRVLFNDLTKDTVTEAIKNPLQLDFNKYEAQQTRRILDRLVGYQISPVLWDKVKR
                     GLSAGRVQSVAVRMICDREDEIKNFKPEEYWNLTAKFEGKNPPPFEAKLHKVDGKKAK
                     VTNGEQAAMLADKIKQAEFFVEKLEKKEVKRTAPPPFTTSKLQQEASRWFRFSAKKTM
                     MVAQKLYEGIELGKEGSVGLITYMRTDSYRISEEAIKDVRDYIRESYSADYLPHKPHT
                     YKNTQKAQDAHEAIRPSRMTNKPQDIKQYLSADQFKLYQLIWNRFVASQMNPAILDQT
                     TIDIAGANCLFRAQGQIMKFPGFTIVYTEGKDDKDENGNGNGNGTDKFLPEVSEKEKL
                     KLLHLNTEQKFTQPPPRFSEASLVRELEEKGIGRPSTYAAILSTIQEREYVKLEKGKF
                     YPSELGMMVTHLLVKSFPTILEIAFTADMENKLDAIETGERKRVQTLKEFYALFADEL
                     KKAKAEMKNIKQEETPTDLVCEKCQSPMVIRWGKNGKFLCCSNYPKCKNTMNFTHDEN
                     GKVKHVETRTTDIKCNKCGKNMIVKEGRFGQFLACSGYPECKNTMTAAKDENGEIVAQ
                     ATPTTDEVCELCGKPMTVKRGRYGQFLGCTGYPECKNIKKLDKDGKAAKQEVVLSDEV
                     CELCGKPMAVKRGRYGQFLGCTGYPECKNIKKIPKTKTES"
     gene            complement(74086..75216)
                     /gene="dprA"
                     /locus_tag="PLR13_01865"
     CDS             complement(74086..75216)
                     /gene="dprA"
                     /locus_tag="PLR13_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQC18104.1"
                     /translation="MNDEILKYWIALKSIKGIGHVSFPALVDRFGSPAAVFGASVSDL
                     QQTEGISRQIAEAIVHFRGWDNVKAELELIRKNDVTIVTYEDELYPGQLLNIYDRPPY
                     IYVRGNLSREDINLAIVGSRAASTYGKYTTERISRDLALQGVTVVSGMARGIDSSAHR
                     GAITAHGRTVAILGSGLDVIYPPENKKLFADIVENGAVVSEFPLGTPPRSTNFPARNR
                     IISGISYGVVIVEAGEKSGSLITARLALEQGREVFAVPGSIDSAGSRGTNKLIKQGAK
                     LIENTEDILEEILPQIEQRKPLKGFSAPGTLNAASPPMEKLNAAEQKLYDLVSQKRMH
                     IDDLISVTGFSSADILSALTTMELKGIVRQHPGKFFSAERTN"
     gene            75424..77157
                     /locus_tag="PLR13_01870"
     CDS             75424..77157
                     /locus_tag="PLR13_01870"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18105.1"
                     /translation="MFKVKNILIFFITIFFIYLPAGFAQNVTKVQKPSVAPKQGVQKS
                     VDQATKKPKPGLTIMPAGINLGLVAFDKSGEATVSLKNTGTGAIRWSIQGPKSWDKVK
                     SKAISGTLEKETDVLNVGVSLLGNDEFKDEKKAGHSLANVEMRIASGEEEIICTKKLP
                     LGIHKEEIEINAADGQKKITLSFFIAYTQSAPRINLSTLRLDMGKILPNKTVSRKIIL
                     NNSGKETLTWSVEPPAHAREDMPDDFRQGRYLSFMNDEVKGAAQYSVPEPLKDTLELI
                     GSWSETGGYPTSGPGDNLIKVNFTGTGILLYLSEHSREGKLTLALDKNPIARAALFEE
                     QRKNGEEILIADQLEFGSHTLTITNKYEGLVVEGVKILGLNTAFFPAQSIKIFPASGA
                     TTLQTNYLTVLFNTGQAFPGFYEDYLVFKTNNGEAIVEMYAEVLPDVTSAIIDVYRYY
                     NGNDYLFTSNPQAEINRLYQNKYTKEGIAFRLFQPGTPGTASFYRWYHPRKKCHFYHY
                     DYNGHKQDLHGYILEGTLGNIATSKLTNTRELYRWYNAKTGHYFYSTELQGEKINKKA
                     YRFDGIAGYVR"
BASE COUNT        19677 a        18127 c        18604 g        20426 t
ORIGIN      
        1 gccatgacgg tcgctgccga tccggctgca tgggacgtca tacatccgtc ggtggtatag
       61 gcaatatcct cgattttgtc atttcttatc ctgagaaaca tttccatcgt atccccgcac
      121 ggtcccgtta ttttggcgta gccgtcaggt tttgtcagcg ttccgcagtg tctcggattg
      181 tttccgtaat cgactacttt atcggaaaga ccgcctgtta ttttcttcat aagataatca
      241 atcatttcct gtttaatttg ctcttcgtcc accctgttgc tccttgttgc tcattcagac
      301 agaataaata aaaaactcgc ggcttatatc tcgcgatcta aagaatttgt ttgccggcat
      361 cggggccgct cctgaccgca tacacggcag taattttgcc cacaatggcc gattttggct
      421 tcagtgtgtc cttgatgccg tgaacccatt cgacgttttg tctaaagatg tcatcgtcgg
      481 tgtggatggt aatatcggcc ttaatttgaa aaccgccttt ttccgcccac cacgacagtg
      541 caattttagg attgctcttc agattattca gtgttttgct aaaatattga tcggaaatca
      601 gcagtgtttc gtcatccata aatttgaatg cgccaatcgg aacgacgttg ggtgtgccgt
      661 cctttgccgc cgttgctaga taagcaattt ttgcagcttt cagactttcc ttaatctctt
      721 ccgtaacgtt taccatcaat tttacctcca aatgaagtga agtgggtgac gaaatcaaac
      781 tgttgtgctg tcttgttctc ccgtgtaaaa aacccttcca tggtcgttga ttatcataaa
      841 ataacccggc agagaaacaa aacttcatcg ggtgtccgcc ggtgccaaag aggactttgt
      901 ttttgcccgt ctggcttgac ggagagttga ttccatcaat aaacgatttg ccatgaaaag
      961 gaaattgtgt aataatatta aattatgatg cagttcattc atcacgatgg ttccgcaata
     1021 aaaatgcagt tacaattatg actgtcggga ggttatgcat gtccaatgta gaaaaagtca
     1081 aagggacaat gctcatagat caggttcgca tgatcagggg gaataaggat aaagattggc
     1141 gcaattatct gacgtctgaa gactgggcaa ttgtcaaggg gatggttatg ccggcctcat
     1201 ggtatcctgt cgaaacatat aagcgatgcg gacgggctgt ttttcaggtt cttgcgggcg
     1261 gcaatacgga tattgtgcgt ttgaggggca aaatgcgcgg gaaagagttg tttgagacaa
     1321 cctacaagca cctcctgatg gaacgctatc cgatgaacgc tcttagttcc tttgtgaacc
     1381 tttacggaca gctttttatg cccaatccgc tgggcttcaa aaaaatgacg gatcagcatg
     1441 cgcatgtatc atacgaatac gaagacaaaa atgacccggg taatttaccg ttttgttatc
     1501 aattaatggg acattttgat tcactgattg aaatgacggg aggcgctgac gttaaaatag
     1561 agtttgcaaa aaaaatgtgg gaaggcgcca gcgaaacggt ttttgatatt caatggcagt
     1621 agcgtttttt agaagacttt aaatcagaaa acatatcttc cggaggtttt gaaatgaatg
     1681 taaaaggcat catttttaat acaggaaaat caacaatcat taaagtattc ggtcaggggc
     1741 cctgggatgc gtttatgacc aaactggtgg ctaaagacaa atttttcggg aatgtaatca
     1801 tgtccgtcac gcccattccg ctggataaat tcattttttt cctggatgaa ctggtcaagg
     1861 aatttttcaa caacgatatg atgcagtacg tgaccttcgg caaagtggcc gcccagttcg
     1921 cgctgtcgcc ggacggtctg tacaaatcat atctgctgac caaagatacc aaacaattcg
     1981 tcgaatcggt tatgcccaag ttctggtcga cctattttga cgaaggcacg gtgaccacga
     2041 aatatgagaa caatatcacc cacctcaaaa tcacaggtct caatttcaga cataattatt
     2101 tcgaatacct gatcatggga ttttttcaga aatcgctcaa aatgttcggg aagaaaaacg
     2161 ttcccaaacg gatcagaagc cgcgcatcgg gcgatgacga tgtttatttc cagtttgaat
     2221 taaaagattc ttaacgggtt gttttgaaac ttcgatcaac gcaaaaagcg gtgcatcccg
     2281 cattgtagag tatccatcat ttgccgttct gatcggaccg cgtgaaatta caagcggtct
     2341 gaccggaaac ccgagggacg ttttttgatg ctgttgatgt gtcgtataaa gcaaacacaa
     2401 caaattgctc tttagatata gttgtgcagg ctggttagaa aattcacacc cagataagta
     2461 aataaaacag acacgaaccc gacaatcgtc atcatggcca tgcgccggcc gcgccagccg
     2521 cggacaaagc gggcatgcag cataatggcg tagataagcc acgtgatcag ggaccatatc
     2581 tctttgggat cccagctcca gtatgagccc caggcatagt gcgcccagac ggaaccggtg
     2641 ataatcccca tggccagaaa aataaatccg agtgatgtgc tctgatagat cagaacatcc
     2701 agtgtgttca gcacggaaga attttccttc ggatgagacg cagggttggt ttctcttgtt
     2761 tctttttttt ccgtccagag ataaaggagt ccgcagccga aagcaaccgt gaaggcggca
     2821 taacccataa agcaggttaa aacatgagtc gtcagccagt tgctttgcag cgcgggaatc
     2881 agcggttcga tgctgtgatc gacccccggt gcgatggaag cgtaagccat gacaaaaaaa
     2941 gccaccggga cgacaaacgt gccgagaatt ctgcttttga tccgccactc aatggccaga
     3001 tagagcatta tgatggacca ggcgaagaaa accagagact catacaaatt ggtaatcggc
     3061 acgtgtccga atcccaggtc gtaggaagct ctccaccgca acatcatggc cagcgtgtgc
     3121 gcgaaaagtc ccagccacgc cagcaaggat gccgcgtatc cccagaattc ttttttcaaa
     3181 atcattctga acagataaaa aacaaaggaa gcaaaataaa taaacgtcac gtaacttaaa
     3241 atcagcgtgt ggttcatgcc gccagctcct tttccatcca tgcgcacaaa tcatcaagtc
     3301 gcctctgcaa cgcttcgtta taacgatgac tgcgcccggc cacgcggatt ctgacgcctg
     3361 ccggatcctg ttccagcaga aaccagatac gccgatacgc aatgaaaaag atgaccatca
     3421 tgccggcgat cagaatcaat ccgccggcca gaacaaacgg gacacccgga tcgcggacaa
     3481 cctgtaaccc ggtgtaatat tttccttcaa tcccccggag agaaaaaacc aggggtttga
     3541 aaaggcccgg attaaaaaga gggacggcgg aaaataagcc ggggtttaca tccgcgatat
     3601 cctgaatatg ctgaaacacc cagaactgga tatttctctt gtccgtttca atatttagtt
     3661 ttacggccgg accgaactgc atcatatttt cttccacgcg caaaacggtg gcctttgcct
     3721 tctgaacggg aagatcaaac gtgtctccct gtcctgctga gatttccggg ctctccgctc
     3781 cggcgttgct gtatgtcaga cgggctctgc ttttttcaga gacaccgtag cttgcctgat
     3841 aaaaacgcag gccgtcaaac gatagcggat gattgaccag cacagaaccc tgacgcacga
     3901 cctgtccatt tttgatgaaa cttaactccg atcgataggt ttttgggact cctgtgtcat
     3961 aaaattccac aacaaatttg tcgcatcgaa cggaaaaatc aagcgggtgt tcaccctcgc
     4021 cattccgcag ctggataatg tttgtttctt cgccttcgga aagtctgaga tccgcctcaa
     4081 gaccgaacac cgagccgatc accatgcccg caataatcat caaaacgcca agatggacaa
     4141 cgtaaacgcc gaacagggat aggcgccccg attcgcgata aagcattttc cccttttcca
     4201 gctttgtttc tttaaccgaa aatagtttcg atttgagaag agattcgacc gcgcggctga
     4261 cggtttccat ctttttatcc gtcacgatta tccggtttgg agcaagattt tcatacagcc
     4321 cggaaggtgg cgggaaagag ggcgtcttgt attgttttat ggcgaaagga attcgttgga
     4381 tggagcaaac catcagattc agtgagaaaa gccccatcaa cacataaaac attttggaat
     4441 gataaaggtc attgagccag agccattcgg aatccattct ctgcggcggc accagcgtgg
     4501 ccacaataaa gatcaccacc atgaaaaaaa gcaaaacgat ggcaagtttg acggaagcaa
     4561 gaaaagacca gatgaaattc gcttttttca atggtttctg cccttaaaga aaagaaaatt
     4621 tttccagaaa acttaacatc taatgtgctt tctgtcaaag agtaccttgc tcagacagcc
     4681 cggacgaact tcttgacaaa acaaccggtt taccttatcg tttaattgtc tttcagtgtt
     4741 ttattgatgt gattgtcgca gcaattcaac aaggaaaatg atatggccga ttccggcaaa
     4801 aagcggttcc tcccacatat caaatctgcc tttgaacgcg tcttatccgg tggatggatt
     4861 tatctcttgt ttgtcggcct gttcggtatt tttcttctca taggaattgg tgccgggatt
     4921 catgcgttgt ttatcgccgg cagccgtcat gcctacgcga catcacggga aattcccgtt
     4981 gccattctga ttgccacgta tgtttttttt gttgtcgctt caacggggct ttgtctggtg
     5041 tcgtcactgg gacatgtctt cggcgtggaa aacttcctgc ccctggcgaa acgttgtgtt
     5101 tacctggcga tcattaccat tctttccgga ttcctggtga tcggcctgga aattgaaaat
     5161 cctttccgca tggcgatata caacgttatt tcgcccaact tctcttccaa tatctggtgg
     5221 atgggaaccc tgtacgggtt tgccgtcgtt tttctgacga ttgaattcat tcttctgttt
     5281 aatgaagcgc accaccataa agctgtcatt gccggtttcc tcggcgtcat tacggaagtc
     5341 gccgccaaca gcaacctggg cggtgttttc gcgatgatgc atgggcgccc ttactggtac
     5401 ggcccttatt tccccgttta ttttatttta tccgccctta ttacgggctg cgcttttatt
     5461 ctgtttttct tttttctcgc ccacaaagcg gtcaacaaaa aaccattcat ggaatcgacg
     5521 caaaccgctc tgaaagccgt atcgaaactg acgctgcttt tgctggccgt catgatgatc
     5581 ttcaccgcgt ggaaggtttt gacgacctcc gttggcagtc ctgccgccgc cgccgcgttg
     5641 agcgtgttaa ctgaaggacc ctattcattc aacttctgga ccattgaaat ccttgtcgga
     5701 atggtcattc cttttgtttt aattctggca tcaagaggca ggaatctgac ggtcatgttc
     5761 atcgccgccg tcatgatgat tttcagcctt ttcttcaccc gtctggatat ggtggttgtc
     5821 ggacagattg tcccgcaata ttttgaagtg ggtgttgtgg aatattccaa attgcatacc
     5881 tattctccga gcattcacga aatccttgtg gtgctgggag ggataagttt ctgcctgctg
     5941 gcatttttgc tgggtgaaaa agtattttcc ggtttcgcag attcaccgca gaccgacatc
     6001 atgaaagaaa aaacaacgga cgttgccgga gtggaagaac tgacatgacg gataaggcga
     6061 gccataaaaa tattccggaa tctccgtttg atgctgaacg gcgggcattt ctgaaaatcg
     6121 gtctgaaaat taccggcgtg ctcgccggcg gctcaatatt gtctttagtc tccaacatca
     6181 acaaggctta ttctcttgat ctttttcggg agaaataccc ttataaacct cattacgcga
     6241 tggtgttaag acaggaccgc tgcattgatt gcgaaaaatg catgaaagcc tgtattgaaa
     6301 ccaatcatgt tccggattac ggctggcgga caaagatttt aaaacgcgac ctttcgtcgg
     6361 cgcttgatca gaaatgggaa tttattccca tcctctgcaa tcaatgcaac aatcctccgt
     6421 gcgtgcgcac ctgcccgacg acggcgacgt ataaggataa gaccaacggc atcgtgcaga
     6481 tgaacaacaa aaaatgcatc gggtgcaaat cctgtattat ggcctgcccc tacgatgccc
     6541 gctattataa tgaagaaaaa ggtgccattg ataaatgcga tttctgtttt gagtccaggc
     6601 tgtccaaagg cattaatacc ccggcctgcg ttgaagcctg tccctcggat gtccgtattt
     6661 tcggagattt gtcggatccg tccagcagag tgtttcaact tgttcaccag ttgcaaagac
     6721 ctgtgtgggt gctgcggcag gagatcggtg cgaaaccgaa tattttttat atgaagggtt
     6781 gatgattcag caatgaaaag aaatcttgtt cttataattc ttctcgctct tttctgcatc
     6841 gccaatgtcg gttctaccgg aaaaagcagt ttttccgacg gcggtggcat tctgtatacg
     6901 cagccggtga aatccgttat tttccgccat cagcatcatg tggatgtaaa aaaaataagt
     6961 tgtgagaaat gccacagcgg cttgtttgaa atgcaggctt tgcttgccca ggagaaaaag
     7021 gattttatta tggattctct ctacaagggc aggtactgcg gcgcgtgtca taacggcaaa
     7081 gacgcttttg catccgataa acagtgcgcc agatgccatg tccgcatcag cgaaaaagat
     7141 gtggggcacg tgaaaggtaa agccaagcca tacaaagcgc ctgtttacag tacggtggcc
     7201 actttgggaa aagaccagcg gaaggtcccg ttcaaccacg aaaagcatgc gtcgctgaat
     7261 tgcatggatt gtcattcgaa actttttcaa atcagaaaag gatcaaccaa gattgccctg
     7321 gaaacgcatc agtcgcaaaa atattgcttt aactgtcaca acgggaaaaa agagttttcc
     7381 tggaacaatt gtacaaaatg ccatcagaac tggaaggata ttaaaccgta cggtattctg
     7441 acggcaagag ccgacagggg aagctgtgtt gcctgccaca ccaatccgga tgaaatgaaa
     7501 tcgctcgtca agccgcccga aataggcggc gaggccgagg ggtgagctgg cgctcctccc
     7561 ccggtccggg cggaagaata ctacaaaggt tatctggtcg ataaaggaat gatcggcaag
     7621 gagttccatc tttcgatgga ttgcattgcc tgtcatcaag gcaacaagct cgcaaaaaca
     7681 aaggagaaag ctcatcaagg acgtatcgcc agaccgtccg acaatcttaa aacctgcggc
     7741 atctgtcata taacagccgg acaaaattac gccaaggccc ttcattatac gagcctgggc
     7801 cagcgcgaag gcgtcaaaca ccggttttct gcggcagaat tgaaaacgtt tgatcagaaa
     7861 gtttttgaaa aatcatgccg atcctgtcac gcttcctgcg gaagctgcca cgtcaaatcg
     7921 ccgcctgtcg gcggcatcac caccggcctt ctccagggcc ataaatttgt gaagaaaaac
     7981 gaaggcaaaa cctgtgcttc atgtcacggt ggaagagtat atcccgaatt taccggtgaa
     8041 tacggcggca cccctgatgt ccactaccag aagggcatga tgtgcaatga ctgccacaac
     8101 aaacaggaaa tgcacggcga cggcaaatta tataaatcaa aaaatgatgt caaagaccgc
     8161 ccgaaatgcc tgtcctgcca tcaggataaa ggcaatcagg tcgcgcacac cattcataag
     8221 gataaagtca gctgtcaggg ctgccattct tccggccaat atcgccagtg ttatagctgt
     8281 catgccgtgc atgggtcaaa ggccgagccc ggctttatcc tggggctgaa tccgcgtgac
     8341 cgcaagacct tgacaaccct cagggtcata cctactataa gaagtacatt tgagcctaac
     8401 ggaataaaaa tggagaattt tgatgctctg cccaattact ggaattcgcc ggtgcacaac
     8461 attaaaaagc gaacaaacag gacacgctcc tgcgatgcat gccatgtgaa taaagaaggt
     8521 ttcctgaaac ctgaagggct gattaaagac ggatctaaag aaaatgagaa actaatttat
     8581 gacattaaac caataagcaa ataaaaacag gaggatcttt atgaaaagaa tgaggttttt
     8641 gaaagtgttg ttggtatccc tgttgatttt actgccggca gccgtgctgg cgcagggact
     8701 cagccctatt gtttcgacag atgatttagc caaaattgta aaggatccca acgtcattgt
     8761 cattgatatc cgcaaggttg aagattataa agcggggcat attcccggcg cgataggggt
     8821 gttttataat aattgggcac cgggcatggg cggcttaaaa aatgaaatgc cggcagatga
     8881 tgatttaatg gaccttctta ccgataacgg cattcaaccc gactcaacgg tcgtcgttta
     8941 cggcactaca ggaactccgc ctgatcgtgt ggatgtgacc cgcgtggcct gggctttgaa
     9001 atacgcaggc gtccagaagg tatctgtact gagcggcgga tttgagaaat gggcggtgcg
     9061 cgagaaaaaa gaagtttcta cagagattac caagcccaag accaaagatt acaaaggtaa
     9121 aatcaataaa gccattctgg ttaacaagga ttacgtgaaa aatcacgtcg gaaaagcgat
     9181 gatcgttgac gcacgggaac cggcattctt taaaggcgaa cagaaacttc ctttcgtggc
     9241 caaagccggt cgagtcaaga attcggtcaa tctgcccacc attcaggttt tcgacaaata
     9301 tccccccggc gagcatatgg agccctgctg ctggacatat aaagatgtcg ccacgctcaa
     9361 aaacattgct tcaggtgtcg ttggcagtga catcgacaag gaaatcattg tctattgcga
     9421 caccgggaaa gttgccagtg cctggtggtt cattttaagt gatgtgatgg gtttcaaaaa
     9481 tgtgaaaatt tatgacggtt ccatggaaga atggatgaat gatgcaagcg ctccgacgga
     9541 accgtaaagc ataaagaaca tcatgaaaaa aagcagcccc atcgttttat ggggctgctt
     9601 ttctattgaa cgttaaacag actttctatt taggcgtgac gttggcgtcg gcgttttttg
     9661 taatcggtgc ctgaggatcc tgcatccatt cctgcatggc gccatcgtat agacggacgt
     9721 ttttatagcc gaggacttcc accagaatat aagaccagat cgggcagcac tgaccgatgt
     9781 cgcaataggt tataatttct ttttccttat ccctgccgat agcggcttcg gaaatcgccg
     9841 cgagttcttc cgtggtttta aatgttttgt caggattaaa ggcgcaggaa gtcggcatgt
     9901 taaaagctcc cgggattcgt cccatcatgg gaatacaatc catctttttc tcgccggtaa
     9961 agaactctcg ttctctcaca tccagcataa tagccttgcc cagccgttct ttaacgtaat
    10021 ctttgtcggc aaatctcgct cttgagtaga ggcctttgaa tggttttgct tttgcgtgca
    10081 tgatctcctg cgaaagcggt ttcttttcag taatccattt attcatcccg ccgttaagaa
    10141 cggctacatt ttttattcct gcgtattgca gggtgcaggc gacacgggcg ctttgataac
    10201 tcagccgcgg cgtatccatg caaccggtga cgacaatccg cgagtcaaac ccgattccga
    10261 agtagcctat ggtatcatcc aggtcatccg tttcggggat ctccgtaaaa agaccgtctt
    10321 tctggtatgc ccatgatctg taaaacgcgt ttagcgcgcc gggaatgtgt ccctctcggt
    10381 aatcctcaac tcggcgaaca tccaggataa tcaatttcgg attcttcaaa tttgccaaaa
    10441 gccagtcggt ggatacaatc ggctcgatgt cacgggtttg accggctatc ggcaataaaa
    10501 ggataaaaag aaggatcgta aggagcttgg acaaagcatg atttttcata tggcaatctc
    10561 ctttgaatgt tgtattacac atcttaaaac ggatgtatac atagtatacc actaattctt
    10621 tattgagaca gccgaatatt tcagaccgca gaatacaagc cctatttcat actattttta
    10681 tcttaccgaa tatgacggtc tatttccccc atcagcgtct gcgtctgatc gggcaacagc
    10741 gcgaatccca tgtcattcct gtaaatgatg ttcatttttt tatcgacaat cacatacacg
    10801 cgtttggaaa ggggcaaaag ccagtggtcg gcgtcgtaga gcttggcgat tttgcctttt
    10861 tcatccacca gcagagtcat gtttttcagg ctgtgcttgg ctataaactt gcggtgcgat
    10921 tcggccgaat cccgcgacag gccgagaatt ttgatttttt tggctttgta ttttttaata
    10981 ttttgttgaa actcaacaaa ctggcggatt caaagcttgg tgtcgtcttt gggatagaaa
    11041 gccagtacca cggcgcgatg atttttgaca gcattgctga gtttccatat gttgcccttt
    11101 tgatcggaca atgtaaaatc aggggctacc tggccggaaa ccggggaggc gttttttgcc
    11161 cctgttgaag cacaggctga caatataaag aacaccagca caaaaaaaac gaattttgtt
    11221 attcgcatat agccctccga taaaggtatg gacggcgggc ggttcaaaaa taaccgcccg
    11281 cccatcttag taaagattaa tcgtcaaacg ccattttggt tttccaaact tcccagacct
    11341 tgcctaccag atcggggccg ggtttgagcg tcggcttgcc gggtttccaa ccggagggcg
    11401 tggcttcggc gcctttggtt ttgcgcacgt gttggaaggc ctgaatttga cggatgcttt
    11461 ccatgacgtt gcgtccgacc ggcggcgtca acacttcaaa gccctgaatg atgccatcgg
    11521 ggtcgatgat gaaccggccg cgggcttcca cgccggcatc ctgaaggtaa acgccaaaga
    11581 cgtctccgac cttgccgccg ccgtcggaga gcatggggaa ggggatgccg cctttgacca
    11641 ttttggaaag ttcatgatca tcccacattt tgtggacaaa gacgctgtcc acactcatgg
    11701 ataaaacttc aacgccgagt tttttgaatt cgggatattt ctcgccgacc gacgagattt
    11761 cggttgctca gacgaaggtg aagtcgcccg gataaaaaca caggacaacc catttcccca
    11821 gataatcgga gagcttcaca ttgatgaatt tccccttgtg atacgcgggc gccgtgaagt
    11881 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcctcataa ccgcgcagtg
    12001 aaatcttgca gtataagatg atttcgcgat ttttatcctc cggcagctcc ttgaggcgct
    12061 tacggatcgc gccgagcggt atcaacgtct caccgatgcc taaacgcatc tcttcgaatt
    12121 cctccggtcc gcgtgtatca atcaggaatg gtttctctcc ggcccgtatt ttatcataaa
    12181 cctctttaca actgatacct ttcattctgc ctttcatttt attctgcatg atgtgcgccg
    12241 tggcgataaa atgatcaatg gccagagaga agggcggcgc gtagggaaga tcggcgttaa
    12301 tcaggtcttc cacggtcatt ttcccctgaa tggccatggc ccactgggca atttgcttgg
    12361 cgacgtttcc cggaccgatg cattgtgcgc cgaggatacg gccggttttt tgctccgcca
    12421 ccagtttagt gaccagaagc ttgccttcca taaagcccgg tttgtcggga ctggcgttga
    12481 tgaccgttat gatgtcggaa tagcccaggc gtttggccat tgtttccgaa aggccggtgg
    12541 agcccgcccc gtaatcgaac actttgcaga tgccggtttg aatcgttccc gggaaagtga
    12601 cgctgttgcc ggaagcggcg ttttctcccg ctattcttcc ctggagattg gccagatcgc
    12661 cgtaaggcgc gtggactttt ttgccggtga tgcggttgag ggtttccacg cagtcgccca
    12721 cggcgtaaat gtccgggtcg gaggtctgca tgtattcatt gacctcgata ccgcccaggt
    12781 ctccgatttt cagaccggct tctttggcca gtgtgacgtt gggacggaca ccgatggcta
    12841 taacggccag ctcgcaggga agctccgttc cgttttgcag tttcaccgct gtgagttttc
    12901 cgttttcacc caggaaagcg gctatgccgt tttcggtgat gatgttggcg cctttgctcc
    12961 gcacatggtt ttccaccagc ttggccagtt cccagtccaa aaacgtcagc agttgaggaa
    13021 gcagttcaat gacggtgatt tcaatacccg ccagttgcag cgcttcgcag gtttcgatac
    13081 cgatgagtcc gccgccgatg accacggcct ttttgatttt gccttcatcg cggacgttac
    13141 gcagaaaatc ggcgtccttc atggattgta atgtggtaat gccctggaga tctgtgcccg
    13201 gaaccggcgg cattcgggga acggcgccgg tggcgataac gagtttgtca tacgtcaggg
    13261 tgccggtttc tccggaaacc gtatttttga aggaaacgga acggtttttc gtgtcgatgt
    13321 tagtgacttc ggtgttgacc ttcgcttcga tatctttggc gttgaggtaa aacatcggat
    13381 tgcgcacgac gcccgcgggc gtgcaaagta gcgcgttgcg gtcgttaaaa aaacctccga
    13441 cgtaatacgg gtagccgcag gacgccatgg aaaggtcggc ttccttctgg ataatgatca
    13501 cttccgcgtc gttgtcaatg cgccgggctt tcgccgctgc tttgggaccg gcagccgaac
    13561 ctccgataac aatgattcgt tttctctgag ccatagtttt atcctccgat ttttaaaagt
    13621 gacctgaaca taaaaggtcg cttccataaa tttgattctc tcagtgaaaa aatagcaatt
    13681 atcggcagcc gttgcattga cttaaatcaa tgcggataat tattttcaga gtccgtatat
    13741 tttgagccgg ccggcatcag accttatgga acatacatgg caacgacctc atccgcagca
    13801 taaattaccc cgccggcgat tagctgtgat ttaataataa cttttacgat gatgcgggcg
    13861 atatttcagc atcaacgtgc ctcaacggaa gcgtgggatg ttttatgaaa gccctgatga
    13921 aaaggggaat ccttcgggat aacggtttgg tgatcgtatc cccgtaggct ttcagcaact
    13981 catccggcat gtagtgaagc tttattcccg caattctgtt ggtgaaggag agcgtgataa
    14041 aacatccggg gcagttgacg gccatatctt tcattccggt ttccttcact tctttgtatt
    14101 ttttccactg ttccttgaag agacttttcg gaagattaag ggtccggaga acgctcacgg
    14161 ccccgcagga aaggttgcac tctccatgat gggggagttc cactatatcg gcgcccgctg
    14221 atttgtaaac ggcgcgaagg tcgcgggcaa agccgtcacc cagttcgctg acataacatg
    14281 attcatggag ggtcgtcttt agtttcaacg gttgttttac tgtgatcttg ccggattctc
    14341 ttttttcatt cagccattca tacagggaaa tcaatttgaa tggcaattcc ctgccgtata
    14401 catttttcag tatattggat agatagttgt agcaggatcc gcagtagcaa accattctct
    14461 cgatattcaa tttctcaaaa gctttcaggg ttttttcaat cgtttcggtg taggctttcc
    14521 atgaaccgag acggtatgca agctcaccac aacagagatc cggcggcccg aatttcggca
    14581 gttcctgtaa aacagcggag ttttcaatat cgaaacaact tattctgccg atgcatccga
    14641 cccacagaat ttcttttgaa ggcggtggaa tttccgacca ttttttcaat atggattgtt
    14701 cctccctgga aagcatggca taaatatcct tccacattga cattttcatg ccgttgaaga
    14761 gataggggta gacagcggat atctttcctt tcttcttcct gttctccatg aacttctcca
    14821 gaataaattc atgaagtctt aacccctcgg ggcaataatt attgcaactg aagcagaacg
    14881 tgcattccct gaaaattttt tccgagtaac cggttttcaa taatccggtc atttcagcaa
    14941 cggcctcggg cttgtccatg tgcatcacgg gacattggac aagacattca ccgcacgtcg
    15001 tacatttatt ccaatccgat aatttggcat atgacataat acatgttccc ccttttctat
    15061 ctgaattgac gaataaccca ttcaagtttc tggatacgcg ccgcagacca tgcaaaaaat
    15121 ttttgccaga gcccggcgct ttttatctca tgcgtcgaat gcgtgtttcg atcttctttg
    15181 tgtgagtgta attcttttaa taacggcagt aaatgcagat tattgctccg ttcatggata
    15241 atgtgccata cgcaatttat ttgcggatcg agcgcgcatt tcaagtttga ttctgaatag
    15301 gatttgcagg ggccgtattt gctttttgcg ggacattcgc tcaacggaca gattccagcg
    15361 gtgcaggtta gcacgcaatg tccgcagctt ctgcagaggc tatgtgcaat aaccggatcg
    15421 ctgctggaaa ctgcggcgct tcccacggaa tccagcacat tgattacagg caaatccggc
    15481 cgacagctat tcgctgattt tattcccgcc gcgcaactca aaacgatcaa tgcatcacac
    15541 ttttcgaggg cctttttgtt ggccctcaac gcctgacgca ttatttcttc gggacaagcg
    15601 gcaagaatga ctttgtccag tttgatctgt ttgtgatttt tcagcagatg cgctttcaga
    15661 acttccacgc cggtcttgcc gcccgtgtag gaaagattgg cgcatgttcc acaactcaga
    15721 atgccgaatg ttttataatc ttttagtatg agattaattt ccggctccgg tttccaaatg
    15781 gttttattca ttgtttaccg tatcctgact gtgaaaagcg tattgtatat cggggcttgg
    15841 gatctgctat tcttttaaat ggtccggcat cagggcttcc ggcacctgaa tggcaacgac
    15901 ttcgccggtg gcgcatatct ggccgccggc aatcagcctc gattcgataa cgactttgcg
    15961 ccttttgatc tccttgactt tcccccgtat ttctatcggt ccgccaagcg gtgtgggcaa
    16021 aagatagttt acgtgcaaag aggcggtcag aaaacgaatc ggcggcaggg tatccatctc
    16081 gcggttttca gagcagtatt tggccgccgc cgctgtgcca gtggaatggc aatcgatcag
    16141 cgacgcaatc agaccgccgt aagccattcc gggaatagcc atgtagcggg gatccggttg
    16201 ataaacacag acggattctt ctccgtccca gtagcttttg atttgcagac cgtgttcgtt
    16261 gaggcgaccg cagccgtaac agtggcttaa atcgtcggga tagtagtcct gaaacgcttt
    16321 ttgcttcata aaaacccctt ttttatacaa gaaattttat atttacaatc gagaggacag
    16381 aaaagcatgc attttttgcg cgtctgttgc aatatcaaca tgcacgtgtt cattgtcaaa
    16441 gatgacattg gttgaacttc tgaaattata cggcaatgcc tcgacggcat cgattgtgta
    16501 aatttttaca tccagaatgg tctcaaattt tgttttcatc tcttcagcta cacctatagc
    16561 cttcttgcag cttattcagc ccggatttcc gctatgaaaa accagtaatg tcttcatgat
    16621 agtccccttt atgttaatta aatgtcaatt ttgttccggc catattcaaa ataaattgtt
    16681 cgggcatttg cttttccatc tccataatgg ctttgcgtcc cgtgcagtgt gtcggaatga
    16741 tataagccgg gttgattttc ttgaattcct gcgtggtgcg atcaataatc ggttcaaaaa
    16801 acggcccgga tagatggaat ccgcccatca ctgcgtgaat tttattaata ccggtaactt
    16861 tcatcgcgtg atgaaccgta ttaatgattc cggaatgcgc acaaccggaa aggataatca
    16921 accctttgtt tttcagattc atgacaatag aggtgtcgtc ttcgatggca tcccaaactt
    16981 cctttccgtc ttgctggcaa tgaggaagcg gccatcccgt ttcgaaatcc gtgctacggg
    17041 gaatttcgcc cagaaaaaga atcgtatctt ccagtaaaag atatggtttt tcagtttcca
    17101 ccacggtgag acccgcggct ttaaccattt cccgcgtcaa ttccggaaaa ttgattttta
    17161 attcctctcc gaatttgaga taacgcggcg atttaaacgc ggacggatgc acggcgaaag
    17221 ggatattctt tttaccggtc atggtgacga gttttgccat gccacccgta tggtcactat
    17281 gtccgtggga aagagcggct gcctcgacct gtgtcatatc aacgttaagc gtttccgcat
    17341 tttgcgccgc accgttttcc gaaaaaccga agtcaagcag cagcgtatgc gttttgtttc
    17401 cgagagttgt ttggacgagt gcggaaaaac cgtgctcagc caacacggaa gcccggattt
    17461 ctccttcttt taaaggattg gctcgcgtga caacagcgtt gctgtccatc gcggtaattt
    17521 caatgtagtt atcctgtaat gtcaggattt caaccctatc aactgctttt aaagctgttg
    17581 tcatgatacc aatcctcctt tgatttagtg atcgcagaga ttgtctccga cttgcaatga
    17641 accggcaaaa aaatccgtaa tcagtttttc cggtgtttcg accggagcac cgactacgac
    17701 ctgaatgccc tgctcggcaa acaactgttg ggctcgttga cccatgccgc cggcaataat
    17761 catattagca ccttgtttgg ccagccatgg cggcaaaagt cccggttcat gaggcggcgc
    17821 ggcaatgtct tcacgtttga tgattttttt agtttggtct tcgatttcga tgatagcaaa
    17881 actcgcgcag tgaccgaaat gcgctgtaag ttttccttcg gctaacggta tggctatttt
    17941 catgattcat tctccttatg agtttaatat ttttgtctta tccaagtctt ttgtaatttc
    18001 atcaatgatt ttttgcatga tttcccctgt gggcgttttc gatgaatgat aaataaaaac
    18061 cttacccgag tcgccggact gtgcgattgc cggatccatg ggaatagcgc caagaaaagg
    18121 cacattcatg tccgttgaga ttttcttgcc gccgccggtg ggcagaatct gtgtaatttc
    18181 accgcatttc ggacacgcaa aaccgctcat gttttcgatg acgccgacca ccggtacgtc
    18241 aagacgcttg caaaagctga tagatttgcg cacgtccact gcggcgactt tctgcggtgt
    18301 ggtgacaata attgcgccgt ccagcggctg aatcaactgg cacaacgaca ggggttcatc
    18361 acctgtgccc ggaggagcgt caacaattaa gtaatccaga tcgccccaat gcacatccgt
    18421 caggaattgt ttgataacac ccattttcat cggaccgcgc cagatgacag cgtcatcggg
    18481 attctgcaac aaaaacccca gagacattat tttcaggcca ccgaattcaa ccggaaacaa
    18541 tccgtcgggg ccgcctttga gagtttcatt ttccagtccc agcatggtgg gtacgctggg
    18601 accgtgaata tccacgtcca gcaaaccgac tcgcagaccg tttaatgcca gcgcggtggc
    18661 cagattgacc gcaacggtgc ttttaccgac gccgcccttg ccggaaagca cgacgatttt
    18721 tttctgaatg cggcagagcg tcgcctgcaa tttgcggcga tcagcgtatt cttcaggact
    18781 ttcatccttt ttctgttttt tagccgcgca gtcggaatcg ccgcacgtat cacatgtttt
    18841 tttttcttcc actattatat cctccattta ttttattgat taagataatc ttcacaagcc
    18901 gcttttaacg tattggtcgc cagaagcgcg cagtgttcgt gatcttcggg aagtccgtca
    18961 agaacattga gaacatcctt ttgacttatt gcttgggctt cctcaatcga ttttccctcg
    19021 gccatacagg ttgtcatgct gccgcaggca cgcgaaggat cacagccatc ggtgataaaa
    19081 gagactttcc tgattttgcc ttcttcaacc agaatccaga actccatggt gtcgccgcag
    19141 ggtccggtaa ttctcgcgtg tccgctaaaa gaatccatcg gtcccatatt atgaggcgaa
    19201 tcggcatgtt tttttgccgt ggcataagtt tcgtccagat ttactaactc actcattatt
    19261 taccttctta tacctcccga agattaagat ttttccagag ttgttgaata tcatcgccgc
    19321 ttggggcatc gatttcaaca accgattttt tctgtatttg cgcctgtgtg acggcattgt
    19381 cgtagcgaat ccgtccggca attcgcgcgc ctaatcccgc agcattttct tcaatacgct
    19441 cagtcatgtc gggattgata tcccatttgt tgacgcacac agctgctgga atttgaaaat
    19501 gacgggtaag gcccagcacc ctttccagat cgtgttcgcc cgaaactgtt ggttccgtga
    19561 ccaccagaac ctgcgaagca ccggtcaccg aggcaataac cggacagccg atgccgggag
    19621 gaccatccac aataatcaat tcacaatttt ctttttccgc gatggcgcgg gcttcccgcc
    19681 gcacggtgga aaccaatttg ccggagtttt cagcggccac ccccagacgc gcgtgaacca
    19741 tcggccccag acgcgtatcg gagacaaacc acttgccgca gttgcaatcc gggaaatcaa
    19801 tcgctttttc cgggcacatt tccacgcaaa ctttgcagcc ctcacaggca acgggatcca
    19861 cggcaaataa aagctctccc tgaaaattct gcttactgtg aatggcatta aagcggcaaa
    19921 cgtctttaca aataccgcag ctgatacagt cggtttgacg gataacagct tcatgaccgc
    19981 tgataaattc gtgttcttcc ttgatttgcg gtgtcaggac taaatgcaag tcggccgcat
    20041 ccacatcgca atcacagatc accgaacgct ttgccagcgc taccagtgat gccgtaacgc
    20101 ttgttttccc cgtacctcct tttccgctga ttattaccaa ttccttcatg gccatcattc
    20161 ctcaatatcc ggcgataggt ttgttctgcc ggccacaagc atgtcctgca agtcgctttg
    20221 atgttctctt acagtgaatc cgcgttcgac caggatggtc ttcatctccg ccagggtgcc
    20281 gccgctgacg tcgtggtatc ttccttcgcg ctcgtgaatc ctgtccaata tcgcgagtcc
    20341 cttttcgtta aaatcgctca acacgatttt accatccggc gatagaagtc gtgtgaattc
    20401 atcacagacc ttgcggatag aggaaaggtg atgaatcatg ttcacggcga aaatcacatc
    20461 aaaaaaaccg tcgttacagg ggatggattc aacatcggcg atgaggaaag tcacctgcga
    20521 ctgaaatcca taatacatca ggttgagcag ggcgaatctc tgttccgcta cggaaatatc
    20581 caaagagaaa aatttatatc ctgcctgcgc cagagcaagc gtgaaatagc ccttgcccgt
    20641 accggcttcc agaatgttgc ccgctacagg caacgatttt tcaatgataa aagaacgctc
    20701 cagatcgata ttgtatccga atgtccggta aagcgcgaca cgtttctgat attttatgtg
    20761 attttcgata atgtcctttt gcatcgtcaa tccttcatct tattttcttt ccgctatctt
    20821 gtaaaacaag ccttgttcgg cgcgcattgc aatgatgagc cgcttatcta aaagactttt
    20881 cagctttttg gccacgtcgt gcggatgcaa tcccatcccg gtactgattc cctcgagtgt
    20941 gcatggtctt ctggccagaa gatccagaat gtctttgtct gaggattcca ccccatttat
    21001 tccggaaaga tcagaggatg ttgcgttttg gagaatgtcg accggacata aaaacaaacg
    21061 ggcaagtttc tgcaattgtc tgacgtcaac gggatgggca aaatcttcat aggctggacg
    21121 gctcacggta ttaatttgaa ctttttcgat atgaatttta ttgacccatg ccgccatttc
    21181 ttttacttct tccgccatac cgttaattcc cgaaagcagg agaatttcca gccagatgtg
    21241 tcctgtaaat tgttttgaaa aatcaatcag cccctgaatc actcgttcga aagaaatatc
    21301 tttgtgcgga cgattgacgt attggaaaag ttgctcattg ccggcatcca gcgaaggaat
    21361 gacgatatcc gccttcatca atgattggcg gacctccggc agataaagca gagacccgtt
    21421 ggtcagaacg gctaccggaa tgttcgtcct ttcttttatc ttaccgatga tttcaccgat
    21481 ggatgcgtgc agggttggtt ctcccgaacc ggcaacggtg atgtaattgc attcaaactt
    21541 cgttaataac ttttcttcga tttccgacaa cacatcagcc aaaggaacat aatcgagcct
    21601 tacggtcgtt ttatttgtcg tttttcccag ttgacagtag atacaatcat acgtgcaggt
    21661 tttatacggc acgatgtcca ctcccagcga ctttcctaac ctgcgggacg ggacaggacc
    21721 atagacatat tgatagactc tttctgacat tttaatctcc acaaggggct tgaccgcatc
    21781 gttgctttgc accgcaacag gttgttcccg tttcttcaaa ggaacagctt tttgcagtgt
    21841 cgcgcatact gacaccggct gacttcataa taaaaccact gccgccgcta acgattcttt
    21901 gtacgtcagc tctacattcg ggacatatcc tgataggcgc atctttcatg ctttgttgcc
    21961 tttcaaaacg tagaccgcac ttgctgcatt catattcata agtcggcatt tgttcttatc
    22021 ctttcttttt cgaattggca attttctcta aaagattttc aaaacgttcc cggtattgag
    22081 gcagggcatc caccatcaga attccccgtg aataattttc cgcaatttgc cggtcatcgg
    22141 gaatttccat cagaatggga acgttttcct ttcgacagaa ttcatgaacg cggttatcac
    22201 cgctccccat tcgattgaca agaacgccaa agggcagatt caactctctg accatatcca
    22261 ccgccagcga taaatcgtgc aaaccgaaag gcgtcggttc cgttaccagt agaacaaaat
    22321 ccgaatccct gatcgcggca ataaccggac aggatgtgcc gggcggggca tctaaaatag
    22381 ccggagcatc aaccggcaac gtatttttta cggcgcggat aagcggtgga gccaacgcca
    22441 cgccaacatt tagttttccc gtaaccagcg tgatattctc cgtgcgcgac gtttcaacaa
    22501 caccgatacg cctgtttttt tcactgatgg ccttagtggg acagaccagc gtgcacccgc
    22561 cgcagccgtg gcacatttcc ggaaacacaa gcggcgttgt tccaaaagtg acaatggcgg
    22621 agaactggca gattttcgca cactcaccgc agccgttgca gagttcttca ttgacatccg
    22681 gcacggggag gccgatggtt ttttcagaat gccccgatgt ttttaaaaat aagtgggcat
    22741 tgggttcttc cacgtcgcag tccgcaagac acacatctct cttaaagaca cgcgccagat
    22801 tgacgctgac cgtggttttt ccggtccctc cttttcctga tgcaacggcc agcttcatag
    22861 gattttactc tcctttgggt tgatccagag cagagggaaa acgatatcgc tggtgcagca
    22921 gggaacccat accgccagaa ataaaaaaaa cgcgatcagt atggtggtaa ctacatcaac
    22981 ggacgtaccc aacgccatta aaacgtgaaa taaggaagcc catgtactca gaagcacatg
    23041 tcccgcatgg ggaattctgg tatgcgatat gacaaaccct aaagcgatgc cggccagcgc
    23101 cagagggttc accagccacc atttttcaat gaagcccaga tgaacgcccc gatgcgggag
    23161 ttccagcagc cattccccga tgtagggaat aaacgaatcg ctgaatgtgg caatgccgat
    23221 tgagccgata taaccgatta tgatgatttg caagatattg ccgcgcgaat gcattctata
    23281 catggcagtg gtaaccaggg cgctcagcaa aacgtgtaac ggatgcattg tccagaaaag
    23341 cccggaggaa aactcccgcg gcacctgaaa ctggatgaat gcagccataa tgacgatccc
    23401 ggttactgta ccgaacattg tgaacggtgc atgcaattta agctcttgcg caatcatgcc
    23461 cgctttcata gggcaactcc tgaagagaaa ccagatatga caaaacagtt tgtggccgcg
    23521 ttcaatccaa taaaaaatcc tgcatgacgg cttttggcaa aatattcggt ctgacgaaca
    23581 aaacaggtta tgccttccga cggcattttc atgaacggta ccctcgtttt cccctttttg
    23641 ctgctgtgga ttcatccacc tcatcctgcc agccgttttt ccggatatca tgcagatcga
    23701 attttgccgt atagggcttg atgtccaaaa gcggagttcc gtcgagaatg tcaattccat
    23761 ccacataaag gatattgtcc tctatacgga tgagttctac aacgctaagc ccaagaggat
    23821 tgggacgacc cggcgcgcgt gtggaaaata ttccccgctc cacgtcctgc agaaacggtt
    23881 tgactttcag ttgaacaagc ggcgcctgat gaaaatgata gatcaggtaa acgtgggaga
    23941 agccgtccag atcgcttaat ccagatatat atttttcaaa cacttcaatg cgtcccttgc
    24001 aaccgcaagc gtaaaccggc tgaatcggtg ttttttcggc aaccgtgtgt tcactgtgaa
    24061 tgattccaat cggatgatat atgatttgtc ggtttatcat acccagtgtc catccacatc
    24121 ggccgatttg gcctcggtta acgtgccttc acgatattgt tccagagcct cgctgaccgt
    24181 agcggcggat gtattgtata tcctgatgcc tgccgcctga agcgtccgga aggctttggg
    24241 cccgcagtgt cctgtaatca gcgcttttac gccaaggcgg gcgatgtttt gtgccgattg
    24301 aatgccggct ccctgcgccg cgttaagatt ctgttcatta tcaacaattt cgaaggtatt
    24361 cgcagcctga tcatagatta gaaatttcgg cgcccgtccg aatctgctgt ccagaggagc
    24421 attcaagtct acgcccgatg ttgtcaatgc aattttcatt aaaaaaattc tcctttgata
    24481 actttttcat agagagcagt gacggcgcta agcggtgact tgcgcgccat cactgcaatg
    24541 catagataag ctctatgcgg gcttcgcaaa agaggctaat tattttgcgt atctgtcttt
    24601 agctcgtcca accgtttttt aatggcgtct aattccctct gcagaaattc agcctgcgtt
    24661 ctgagagcct gttgttcctc atggggatca ggagggacaa ccgcggcgga gtattcatcg
    24721 gttcgtcgcc atcccggtat tgcagcggca taaaaacggt tgccaaagcc gaatccaccg
    24781 ccacggccac ctcggccgcc aaaaccggcg ccacgtccga aacctgctcc aaaacgtctt
    24841 gccggaatat gattcatata accgggcgct ccgaaacctg cgcaatatcc ggctcctcta
    24901 cctgtcatcg gtcccattcc aatggggcct gttgcatctc ctcttggcat aatgaagacc
    24961 tccttttttt agttattccc gaatcatggt tgaaccgcac tgcgggcact tgacttgcgt
    25021 gcagggaatt cccctatcat gcggttgacg gttaccgcat ttcgggcata gacagttgcc
    25081 caaagctccg cccgccttca tccctcccat gcgtcctcgg ccaccgccgc cgcgtcctcg
    25141 gcccataccg gaaccggaac cttggctcat accgccgccc gttcctcttg gatttctatt
    25201 atttgtattc atgatgtttt ctcccttttg gcgtctcttt tttagaaaac gaaaactgcc
    25261 gccgccagta ccgtggtata gtcttttgtt ttcaaaaccg tcgattgcgt tgtatttctc
    25321 gttccgacga ttttaccgct gattttaaac agttcctttt tctcgtccca gctttcatca
    25381 atgttgaaat caatccccag cgtggatgcc agcatcgctg cggccaaatc ttccgcatag
    25441 tctccggtct gtttttcgtt ataaccgaac gaatgatgct cgctgatata accgtacgtt
    25501 ttcttatcca tgggaatcgc acagccgacg gacgccgaaa tcaaacggcg cggctcgttg
    25561 ctgcagcagc ggctcataac gcagaaggtg atggcgccgg gtnnnnnnnn nnnnnnnnnn
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnaatcctgc gcccatcgct cttctccggc agccgggcaa
    25741 agagaacaca ggcaatccca gcgtgttgtt ccggtaggcg gttataatgg catggacctc
    25801 tcccctgaca aagggatgaa cgatgattcc ctgggccata atcaacgcct gcataggacg
    25861 ggaaatggcg ccgcaaatta gaacatcgac gccccgattt tttagttcag ccgcacgccc
    25921 ggcaggatcc gtcgcgacga atttctgcaa tgttctttta tcaggattcg ccgtgtctgc
    25981 ttcaacaatt aaaagttgct ccgcggaatc gaaaaccgtg gaaatgcaat ccttaaatac
    26041 cgacagtgcc agcttcatta atgctcctta ggatgaaata cctatagaac attcatagca
    26101 aaatcaatgc cagaaggtgt ttttaaaatt tagtgtttga ttacaggtta ttggaaatga
    26161 cggttattcg cttccatgaa aaataagtag cgttaatgca acgatagttg caaaaagtcg
    26221 caggaatgcg actacagagg gaattgcccc ggaagggtaa tgccgagtgc tttgattttt
    26281 ctgtaaaggg tgcttttatg aatgcccagt tgccttgccg tttcccggca attccagttg
    26341 tttttttgaa gagcgttgat gagccaggcc gcttccacgt cacgaagaga ggacgcatcg
    26401 gttctgattt tctggggcgg agacgcaaaa agagattccg gtagatgctt tttttcaatc
    26461 cggccttctt tgcagaagat gaaggcgcgt tcgataatgt tggccagttc ccggatattg
    26521 cccgggtaag tgtaagccat cagaacggac agtgcttcct gactgacgcc ttcaatctct
    26581 tttttttgca gaagatcaaa gcggctgata aagtgatcaa tcaacagtgg aatatcttcc
    26641 atgcgttccc tcagcggggg aagggtgatt ttgaagacat tgatgcggta aaacaaatct
    26701 tcccggaaac ggccctgtct gaccagatcc tcgagatttt tattggttgc cgcgatcaca
    26761 cggacattgt gctcaacgct ttgtgttgcg cccagcggtt cataggtttt ttcctgcagg
    26821 acgcgcagga gcttaacttg catggcgggg gtgatttctc cgatttcgtc gagcaataac
    26881 gttccgtttt ctgccagttt aaaacggccg ggcttgtctt ttttcgcgtc ggtaaacgcc
    26941 cccgctttat agccgaacag ttccgactcc agaagtgtat ccggcagggc gctgcaattt
    27001 accgcgacaa aggacgtttt gcggcgcgga ctcaggttgt ggatggcccg ggcgatcagc
    27061 tctttgccgg ttccgctttc gccttcgatc agcaccgtac tgccgcctgc cgcaatatcg
    27121 ggcaggatat cgaacaactg cagcatttta tgattcttgg aaagaatatc ggcaaagttg
    27181 tatttctgta aaaccgcctt gcgtaaatct tcttcgacgc tgatgtcccg gaatgtttcc
    27241 accgctccca gcaattttcc cttttgatcg cgcaggagcg ccgtggaaat actgacggga
    27301 agtttgtgcc cttccgcgtt gatgatggct acttttttat tgatgatctg ctcgcccgtt
    27361 ttcattgtct tgcgcaggca gcagtctttt tcgcaaatat ccgcttttaa aacatccttg
    27421 cagcgttggc cgatggcttc ctcccgggga accccggtta ttttttcagc ggcacggttg
    27481 aaagacgtga tcagccagtc ctggtctacc gtgaaaacgc cgtcggcgat cgaatccaga
    27541 ataatatcct gtttggttcc gatatccggc gatccctgtt ttaaaggcat cttttttttc
    27601 atgtctgttt tccgatgacg ggtgtcggat ttagtgacag ccgtgttcgt gctgggtgca
    27661 ggaaattccc gaatctttca gtgtgccggc aagccagctt tccgtaactg tcttcacgtt
    27721 tccggaacag ccgcgcagaa catcaatgcc atggctgttc aacacattga cggcgccctg
    27781 tcccatattg ccggccagca tcagcttgac gccctttgca gccagcactt cggcgatgtt
    27841 ggatttacac ccgcagccgg cgggtgaaga aaccttttcc tcctcgacaa ttttcttttc
    27901 ctggtcaatg gtgaaaaccg taaaatactc acagtgtcca aagtgttcat caatctgatt
    27961 ctgataagag ggtaaagcaa ttttcataca tatctccttg caaagcattt ttcatttatt
    28021 cctgcgtttt gaatgttcgc gggttttcta ttcatcaaaa tcgaagtcgc cgccgattct
    28081 taatgctttt ccgttgatca gcacatcggc gatctttttg tgtgcgacgt ctatgattct
    28141 gccgaaggtt tgacgtgaaa tgttcatctt ttcggcggct tgtttctgat acaaatgttc
    28201 gtagtcggca agacgaaccg cctcgaattc gtccatattc agaacaattt cttcgagctc
    28261 catggagggt attcccctgg gcttgaagta tttcttgtcg ggcgcaccca tgatgtgacg
    28321 gtggcatttc ggccggggca ttgtttgtat gaactccttt tgttagaata taagcatatg
    28381 ctcaaaactt gtcaagggtt aaaatttcct cgtaaccgta tttaaagtcg ttaacgcgag
    28441 ttttagaaaa gataatcgtt ggaggaattc ctactgcccg ataccatttc gcttcttccg
    28501 gtgaccgtaa gaggacattt gattgttgtg aaaaaaaaac ggctgtggta acttcttgcg
    28561 caattcgatt tttcaaagcg cacaagggtg ttttttcatg cagaagcata tcagcaacgt
    28621 tcacgtgcac atgccttatt ctcttttacc gaagtatttg aagatcattc ttcagaacaa
    28681 actgaatctg gaaatatact ttcatcacca gattctgaat cgtctggata aatctcgatg
    28741 catggaaacg gcaaagattc ttgaaagcga gggattgaaa ataacgtttc atgcgccttt
    28801 tgtggactta cggccggggg ccctggatga gacgatccgc cgggccagca ttgaccgcat
    28861 caggcaggtt tttgatctgg caccctattt tcatcccctg aaaattgtct gtcatgcgtc
    28921 ttttgacgac cgttactatg tttatggcga cgatttatgg ctagagagca gcctcaaaac
    28981 ctggaaggag ttcatcgggc tggcggacga tcaccggacc atcattgcgc tggaaaatgt
    29041 ttacgaaaaa aatcccgtga tattgggcaa gcttttccga tctctggcgt ccgacagaat
    29101 ttgtttttgt ttcgatacgg gccattttaa tgtgttttcc tgcgaaccgt tgaatgtctg
    29161 gttcgatgaa ctgggaaggt atacggggca tttgcatctg catgacaatt tcggatggca
    29221 ggacgagcat ctttctgtcg ggcacggcac gtttcccttt gcggagttga tgcagtacat
    29281 cagaaatatg aagaaaaaac cgacggtcac gctggaggcg cataatcaga aaaatctttg
    29341 ggcatcgctc gatcatgtca aacgaatgga tttccccggt gtttgaagat aagacggact
    29401 gtgttggggg agtattgtgc tgaggtatgt cttaaaaaga acattgttca tgattccgct
    29461 gttgatcggg attaccctga tctgtttttt cgtcatgcgt ctggcgccgg gttctcccac
    29521 ggatttgcag acggagatga atccgaaggc atcccaggaa agcaaagagc ggttgaatgc
    29581 gctgtatgaa ctggataagc cgatttatgt gcaatacgga tcatggctga agaagatcag
    29641 ccgcggcgac ctgggcgtat ccttctcgtc cgatcaccgt ccggtggccg acaagatcat
    29701 ggagcggctg cccattacca tcctgatcaa ttttttatcg ctggtgatca tccttgctgt
    29761 cgcggtgccg atcggcgtgt tgtccgccgt ccaccaggac tcgcttttcg ataaggtgac
    29821 caccgtgctg gtgtttatcg gttttgcggt gcccacgttc tggctggccc tgctgctgat
    29881 gatttttttc ggaatccatc tgggctggct gccgatttcc ggtctgcgct cgctcaatta
    29941 cgaatatctg aatgtctgga atcagttgat cgatctggcc aaacatctgg ttttgccggt
    30001 tttcgtgtca gcgttcggcg gattggccgg tctgtcgcgt tatatgcggg cgaatatgct
    30061 ggaggtgatt cggcaggatt atattatgac cgcgcgcgcc aagggattga gtgaaaggga
    30121 tgtcatttac cggcacgccc tgcacaacgc gctactgccg gccatcacta ttttggggct
    30181 ttccattccc ggtctcattg gcggcagcgt tatctttgaa acaatttttg ccattccggg
    30241 catgggacag cttttttaca tgtcggtcat ggcaagagat tatccgacca ttatgggaat
    30301 tttgctgatc ggcgctattc tgacgctggt gggtaatctg attgccgatg tgtcgtacgc
    30361 ggtggcggac ccgcgtatca gaatatccta aaagggctga aaagatgacg gcatggacag
    30421 ccatctataa aaacaaactg atgttgaccg gcggcggact ggttctggcg cttttgatcg
    30481 tttccattct ggcgccgtgg ctgtctccct atgatccggg acaaatcgat ttgaacaatg
    30541 tgctggcagc tccttctatg gagcatcttt tcggaacgga tcaactgggg cgggatgttc
    30601 tttcgcggat gatctggggt gccagaattt ctttaaaagt cggttttgtg gcgacggggt
    30661 tggccattct cgtcggcacg tttctgggag cattgtcggg atattacggc ggctggatcg
    30721 attcggtcat catgcggttt gtggatatca tgctctgttt ccccacgttc tttctgattc
    30781 tggcggtcat tgctttgctg gagccgtcca tctggaatat tatgatcatt atcggactga
    30841 ccggctggat gggcgtaaca aggctggtcc gggcggattt tatttcgttg cgggaaaggg
    30901 attttgtcca ggccgccaga gcgataggcg ccggggatgc gcggattatt tttcttcata
    30961 tccttcccaa cgcgatggcg tcgattctgg ttgcggcgac gttggggatt gcgggggcga
    31021 tcctcaccga atcggccctg agttttctgg gtatcggtgt gcagccgccc acccccagct
    31081 ggggcaatat tctgacggcc ggcaaggata atatcgacat cgcctggtgg ctgtcgcttt
    31141 atccgggcct ggccattctg acgaccgttg tcggttacaa tcttctgggg gaaggcatca
    31201 gggatctgct tgatccccgt ttaaaaaaat aaaaccgggg ccttgtcaac cggttcattc
    31261 ttgataaaag gacgaaaata taataataac ctcacaacga cctatacgta aggagaagaa
    31321 accatatgcc caacagagtc aatgcagaat ccatcagtga cgataaacgc atcctgaagc
    31381 gcaagctgat gcagggcgaa atatccgaaa aggatataca ggctctaata aagaagcttc
    31441 ccgatgtttc cgaaaacgcc gaggaagttc gcctggatga tgacaaaaaa tagtatttta
    31501 tgctgattga ttcccatgct catctggaaa tgaaggagtt tgatgcggac cgtgatgacg
    31561 tcattagccg cgcccgaagc gagggcgtag attttattgt cacggtcgga accaatctcc
    31621 ggttgagcag cagagccgtc gcgcttgccg aacaatacga aaacatcttt gccaccgtcg
    31681 gcattcatcc ccacgatgtc gccaaagccg gtgaggacac cctggatgct ttaaagcaac
    31741 ttgttctgac tcataaaaaa atcgtcgcct atggtgaaat cggacttgat tatttccgga
    31801 atatttcacg tcaggaaata caagtcgaga tgttcgggca gcagctggac ttggtgaagg
    31861 aactgaatct gccggttgtg attcatgatc gtgaagcgca tcgtcaatcg ctggatatga
    31921 ttcaggcctc cggtgtgcgt cgcggcgtct ttcactgttt ttccggggat tatgacatgg
    31981 ccagaaaatg catcgacctc ggattctaca tctccatccc cggtgtggtc acctttgaca
    32041 aatccgggaa aattcgcgat gtcgtcgaac gggttccgct ttcctccctg ttactggaaa
    32101 cggacgcgcc gtatctgacg ccggttccga accgcggcaa aagaaacgaa cccgccttta
    32161 tcgtccatac ggcgaaagcg gtggccgcca taaaaaaaat atcatgggaa gacgtggcgg
    32221 cgcaaacgtc tgttaacacg atcaatctct tcggtctgaa gatgatccgt aacgtttagt
    32281 tttttacgga gccccgttca tgaagaaaca atttaaagcc gtggttttgt taagcggcgg
    32341 tcttgattcc gccacaaccc tggcgatcgc ccgcgacagg ggatttgaaa tttacgcgtt
    32401 gagtttccgc tacggccagc ggcacgcggt ggaaatgaaa gctgcccggc gtattgcacg
    32461 aaaaaacaag gtcgccggac acctgatcct gaatatggat ttacgcaaaa tcggaggatc
    32521 ggccctgacg gcggatattc atgtgcccaa gaatcaaaca ggctcggtgc tgaaaaggaa
    32581 aattccggtg acctatgttc cggcgcgcaa cacgatattt cttgcctacg cgctggcctg
    32641 ggcggaagtg atcggcagtc atgatatctt tatcggggtc aatgctctgg attacagcgg
    32701 ttatccggat tgtcgtcccc ggtacattgc cgcatttcga aagctggcca atctggcaac
    32761 gaaggcgggg gttgaaggaa aacaaaaact ggccattcac gctccgctca ttaaaatgag
    32821 caaggcggag attattaaaa agggcatgga ccttggagtg gattacagtc tgacgcacag
    32881 ttgttatgat ccgtcgccgt cgggagagcc gtgcggtgaa tgcgattcct gtctgctgcg
    32941 attgaaaggg tttcgtgaag ccggattgaa agatccgctg aaatattgcc ggtaaggggc
    33001 tggtcttggg ttatcgaatc aaagagatat tttattccat tcagggcgag ggattttata
    33061 cgggacggcc tgcggttctc tgccgttttt cggggtgtaa tctatggtcg gggaaagcat
    33121 ccgaccgcga aaaagcggtt tgccggttct gcgatacgga ctttgtcggt tgcgacggcc
    33181 cggagggcgg gatttttcaa acccctgaag cattaaccga gaaaattatt acattgtggc
    33241 ctgaaaaacc gtcagacacg cttaggccgt ttgtcgtgct cacgggagga gaaccgctgc
    33301 tgcaaacgga tagagcctta ctggacgctc tgcatcatca gcatgttgaa gtcgccgttg
    33361 aaaccaacgg caccattatg ccgccggaag gaattgactg gatttgtgtg agccccaaag
    33421 caggcacaac gatggtgtta cgttcgggac aggaattaaa gcttgtttat cctcagcagg
    33481 gagccgagcc ggaaaagtat gcgctgctcg attttcagca ttattttctg cagccgcttg
    33541 attcaccaaa ccggcaaacc aacaccctca gggccctgga atatgttttg gcgcatccgc
    33601 aatggcggtt gagtttgcag atacaaaaaa ttttaaatgt aaaataaagg aacgcaaagt
    33661 ggaaatcttc aaggtgtata aatttgatgc ggcgcaccgc ctgccgaagg ttggtgcaga
    33721 acacaaatgt tttgaactgc acggacattc ctttcgcgtg gagattcatg tgcaaggtcc
    33781 ggtcgatccg cagctggatt gggtgatgga ttttgccgat atcgaccgtg ctttccggcc
    33841 tctgctggat cagctggatc atcagaattt gaatgacatc cccggactga ataatcccat
    33901 ctcggaaaac attgcgcggt ggatctggga aaatcttcag gacgcattgc cgcaattaaa
    33961 caaaatcgtt gtgcaggaga gcccggaatc cggctgcatt tattgcggcg aaaaataacg
    34021 gtttggaggc ggggatgctc cgcagaaaat atttccgttt agccgccgaa acgtcacgga
    34081 tatcttccgt ttcagagcga attaccagcc ccttttttgt tgaaaaataa tgcggtcggt
    34141 ggtaggtgct gaagcaaaca aaaagcagtg accatcatgg atcagaaggc catacagaag
    34201 atcaaaggca cccagcagga cggtttgctg gaagaattat tgcgggaaaa ggccgcggtg
    34261 ctgacccggg cgagctgggc ggttgaggat gtgctggaga agatgaagcg aatcgaagga
    34321 gaaatcgaaa ttaaaacatc gttgatccag tcgatgacgc acgaggctga tcgcgcggtt
    34381 ctgtcccgtc agaaaaaact gttctgcgaa gaaataaact gtaagataga cgaattcaat
    34441 gccattcgcc ggaaagccca gcttcaatat tattatttga ttgtcacaag agaagccctg
    34501 ggcttgaggc gtcatgagat ggttcagaaa atttaccgta ttcccgacaa gaaaaaaaag
    34561 atacaggtgt ttttttaatg gacaggttcc gtaaaccacg catgcggatg gtggaaacgc
    34621 agatcaaggc acgtggcatc agcgacgtgc acgtgttgaa ggccatgcaa acgattccgc
    34681 gccatctttt tgtcgatgaa ggcctgatcg aacaggcgta caccgacagc cctctgccca
    34741 tcggcgaaca tcagaccatc tcccagccgt atatcgtcgc gctcatgacg caggcgctgg
    34801 atctgaaggg aagggaacgg gtgctggaaa taggaacggg atccgggtat cagaccgcgg
    34861 tgctggcctg tctggcggac cgcgtttttt ccgtagaacg cattgccttg ctggccactc
    34921 aggcgcgcag aacactcgac gcgctgaata tctataatgt ggccatcaga gtgggtgacg
    34981 gttcttacgg ctggaaggaa gaagcgccgt ttgacgccgt tattacaacc gccgcggtgc
    35041 cggcgattcc ccggtatctg ctggatcagt tggttccggg gggaagaatg attgtcccgt
    35101 cgggtggccg cgatgtgcaa accctctata aactaacccg atccaaggaa aatcctgaag
    35161 agattataca agaagacctt ggaggctgcc gcttcgtcag tttgataggg gagagcggat
    35221 ggagaaaata gcaacgcggg caaaaccggt tttttnnnnn nnnnnnnnnn nnnnnnnnnn
    35281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35341 nnnnnnnnnn nnnnnaccgg cgatcttcaa gattccgcaa cggcaaaaat ccgggacgac
    35401 aaaccaacgg atcccaaaaa taaaacggtt gaagaaatgg ttgtcccgaa ccggaaaata
    35461 aaagcgacac acaacaaatt tctctggccg gtgcagggcg aaatcaaggc gcatttcggc
    35521 attcaaccca ataaaacgtt ccataactgg gttaaaatcg tttctgcatc gggaaagaaa
    35581 atcaaagcgg cggaatccgg cacggtcatt ttttcatccc atctgaagaa ttacggtgag
    35641 accattatta tccgtcacaa agatcatttc gccacggtct acacccatct gaaaaaaagg
    35701 ctggtcaaaa tggatacgcc tgtcaaaaaa ggcgaggcga tagccgtcct aggggaaaaa
    35761 gacgatgacg gggcggttta tatgaatttt gaagttcgtc tgaaagggaa agcggtcaat
    35821 ccgctttcat atctgccgta attttttgcc gttgccatga cgcaaatcat ccttatatta
    35881 aacaaaaaaa ttattcaggt taacgatgaa atgctatttt gaacagggcc ccattcgtcc
    35941 tcccagtgaa gcggcaagcc ttctgatccg cgtcagcaga aattgtccgt ggaacaaatg
    36001 cgctttctgt catacctatc gggggacgaa attcgagctg cgcagcgttg aagacgtcaa
    36061 aaaagacatt cagaacatga aagagatagc cgatgaaatt gtccgttttt cctggaaact
    36121 gggggagggc ggccgggtca accgcaatgt tttgcgcgcc atctacaaca gtgaagattt
    36181 ttatcatgac aatatttatt ctgtggcggc ctggctttat ttcggcggtg aaaacgtgtt
    36241 tctgcaggat gccaattcgc tgattttaaa aaccccggat cttgtttcca ttcttgattt
    36301 tttaaaggaa aaatttcccg atgtgcaacg gataacatct tactgccgtt cccacacggc
    36361 ggcaaggaag agcattgcgg aattcgaaca attgaaaaaa gccggactgt cccgcattca
    36421 tatcggcatg gaaagcggct ccgacgaggt attgtccttc attcgcaagg gagtgacgtc
    36481 tgccgaccat atcagggcgg gactgaacat cagaggcgcc ggcatagaat taagcgaata
    36541 tgtcataccc ggactgggcg gagaacggtt tacccgtcag catgctctgg aaaccgcccg
    36601 ggtcataaat gccatcaatc ctgattttat tcgtctgcgc accctgcatg tggtgccggg
    36661 caccgatctg gatgagttga tgaacaaagg tgaattcaaa ccgctgacgg atgacgatat
    36721 tctccgggaa ataaaattgt ttattgagaa tcttgacgtg cgcgggacgt atctggccag
    36781 cgatcatatt ctgaatctgc tggaggaact ggaggggagt ttgccggatg accggcaaaa
    36841 catgctggct gcgattgaaa aatattttaa actttccgcc gaagacagac ttgttttccg
    36901 actgggacgg cgcagcggat cattgaggaa actcgatgat ctggccgatc aggcaacgta
    36961 cgggagactg aggtcggtcg tcgatcacta tgctgctcga ggcgaaaatc tcgatgagga
    37021 tatcatcaga acgatgaata atttcatata aatatcgggg aaatattttg tcgtccgtac
    37081 agccttttct tttatgtcat cctgattcgt tttattttgc catgtggtaa atattttatt
    37141 gacacgaagg ttctttctgc ttatacttcc gccgtcaaaa ggcaatattt tatcgcgtat
    37201 caggcaatcg gcgtctcgca ccgcgtctgc tgatgatctg agaccatttt attcctggtt
    37261 tcgaaagagc ttgactattt gcagctccat aatggtcggc agaaaagtta ttctgcagaa
    37321 cattattcaa tgctacagga atgtgatccg caggattgcc cggtaggttg ataaaaggat
    37381 gcaacgtttt gataaaacat caaacaataa tttaaggagg ctatacaatg cgtgaatgtg
    37441 taattattga tggcgtgaga tcgccaaatt cccgggcaca caatgaaaaa ggttggttca
    37501 gagccctcag acccgatgaa gttctgacca aagtttatga tggtctgttt gcccgtaacc
    37561 ccaaagtgaa acccgaagat atcgaatccg tatttgttgg ttgcgccaac attaccggca
    37621 tgcagaatga tatcggccgc ctggcctggc tggccggtgg atttccggaa tccgttccca
    37681 ctaacacgct gaccatgcag tgtccttccg gtatggcagc cacgcagcat gccgcccgcg
    37741 ccattatgtg cggagaaggc gatacctaca ttgcttccgg cgtggaagac atgcagaaag
    37801 ttttcatggc catgcacatg gatttcccgc cccgtctggc caaccgttat aacattcttg
    37861 agctccccat gggcaatacc gcagaaaagg tcgcggaagt ttataaagtc agccgtgcag
    37921 atatggaaac catggcgtac tggagcaata agaaagccga cgaagccacc aaggccggaa
    37981 aatttaaaaa tgaaatcatt ccgatggaag gattaaaaga tgacggcaca cccttcatgg
    38041 tcgaaaccga ccagtggatc cgtggtgacg tttcgatgga acagatggca accatgaaaa
    38101 ctcctttcaa accggatgga gttgttacag cagccacttc ttcgcctttg acaatcggcg
    38161 ccgcggccat tcttttaatg gaacgctcca aagccgacaa actgggactt tcttacagca
    38221 ttaaatatga agcaggcgct ttaggcggtt gcgatcccac cgtgatgggc attggtccta
    38281 ttcccgctgt ccagagactt ctggcgcgca caggcaagaa agtcagcgat atcggtgtat
    38341 gggaactcaa tgaggctttt gccagccagt ctctggcatg tctgagagaa ttgggcattg
    38401 cccagaatgc tccttttgac aatgtaaaca tctggggcgg cgctctggcg ctcggtcatc
    38461 cgttgggaga aagcggatgc cgcattctgg ttactctgca cagtattatg aagactgacc
    38521 gaaaagatgc caaatatggt gttgcttctc tttgcggcgc tttcggcaac gccggcgcca
    38581 ccatgttgag cagagtctaa tacgtgatct gctaaacgca tccatgaaaa agggagttcc
    38641 tgatatcagg aactcccttt ctttttttta tgatgaattc tatcgaccaa tcaattttta
    38701 catttttccg gaaagaaaca gatcggcatc gcgggctgtt tcatcgggga tttcctccat
    38761 cggcgtgtgt ccggcgcctt cgtattgtat gaattttgct cccggcaatt ctttcttcca
    38821 gttttcgaaa tatttaaaag gaatccattc atccctcgtt ccccacataa ccagcgtcgg
    38881 gacgcggatg tcttttatgc cttgggagag attttcatcg cggcacaatt ttctcatttc
    38941 cgtgaaaaca tcaatatagg atcctttgtt gccttctctc atggctagat caaaatagcg
    39001 gtctttgagt tcctgggtca ctttggattt gtctccgaaa acctgttcaa ccgcgttgtc
    39061 gaaaagaaac cgcggcatcg taaacctggc gaacggtcgg ataagcggat tgctggcaaa
    39121 agcaaggagt cccggcagcg gctggggaaa tcccacggaa tcaaccagaa taagcttctc
    39181 aaccctgtcc ggatgtttca gtgtgtaatt ccaggaaatg tagcctccca gagagtttcc
    39241 cgccagataa aacttttcca gtttcatttc atcaacgact ttttcgaaga gcctgaccgc
    39301 ttcgtctttc cggtacagag atttatcggg ggccggtccg gtcagtccga atcccggaat
    39361 atccagccgg atgatacgat agcgatcttt cagccgctgt gtccatccgt cccaggtgtg
    39421 cagggacgca cagaccccat gcaacaggat cagaacaggt ccctgtccct cgtctttata
    39481 atgaattttc atttcatcga tttcgatgat ttctgattct gcatcggcat atttcagttt
    39541 cagtttttcc accgggacag tgttccatcc cagtgccgta cagcccgacg tgaccgccat
    39601 gatacacaat aacaaaatcg gaatgagtgc tttgagcgtt ttcatttttt cggcctttca
    39661 tcaattgtat ttaaacaacg cgaggatttt gacaagaact atagggtttt cgatttttta
    39721 tgtcaataaa atatcaaatc ggaaacagaa ctgaccggga gtccgttcga aaaacttttt
    39781 ttataaaaac ttattgtcaa attcattgtc atgctttata ttaaaatcaa acattcaaac
    39841 gaattcgagt tagaaaggag gctgttatga aaacctatgc cataaagtat ctcgaactgg
    39901 cagaaaaaca cgcggaaaag atagcggcga gatggttgaa agacgtaaaa aacaactcta
    39961 ggacaccgac ttataaaagt ttgaatgaag cagaaatcac caatcaatgt gtcaggtttt
    40021 atcaaacctt cagtaaaatg tttcttgatg aaaaaataac ggatgattca ttgcgctatt
    40081 tccggagtta tgctgaggaa agtcatacca tgggcatccc cgcaaaagaa acgctgtatg
    40141 ctctcattct gatgagacgc catatctggc tctatgctga atttcaggcc atctttactt
    40201 cagggattga tcaacggcag gccctcgata cgctcggacg caccatttta ttgttcgatt
    40261 acgcctgtta tgaagtaacg aaggaatacc agtcactgac gagataacga taaaataaaa
    40321 aatatgtcag caattattta gtgccggact taatctcata gaaaggagaa tattgttatg
    40381 gtcgactcta taaagttact cgatattacg gagcaaaacg ccgaagaaat cgccgagcac
    40441 tgggcgaagg aagttcataa aaacaagaga actacccatt atcgaaacat aaaaaaggaa
    40501 aaactggtcg tttacgcagt ggatttttac cggaatttac gaaaattgct ggttcccgag
    40561 gatcgtcttg aatatacccg ggaatatttt cagaaatatg cgaaaaaatg ccatgaaatc
    40621 ggtcttccct tacaggaagc gatttacgga cttgttctca tgcggcgtca catgtggctg
    40681 tacgccgatt ttcaggccat cttcatcgat gccctggaac acaatcaggc gatcgacagt
    40741 attatgcgaa tcatgctcat gatggattat gcggtttatg agatcaccca gtattatctg
    40801 gaaaccgcaa agcattgatt ttctgaaaca taacgtaaaa acgacaaaaa aagtccccgg
    40861 aatcagatta tatcagtcgg ggattttttt atttgataac ggaaaagtcc cttaatttga
    40921 aatcgttaaa atttatataa gtatttgata atgtattgtt attatagaaa tctccggaaa
    40981 aagtaaaata aaatacttaa atctcaataa tatgttgaca gagtgaattt tgggatgtat
    41041 aacacgcaat acatgacttt cagtcatttg atataattat attaaacaat gtaaaagatc
    41101 gtccgttttt tacattttcg catgtcagaa aaattattgg gaaaggtgtc tagagtctcg
    41161 tcgttcagtc agaacgagta caaaatacaa taaacaaagt ttaaggagga ttatatggcg
    41221 agtttgtggg ttgatttacg ggatattaaa ttccagttgt ttgaagtatt aaagattgag
    41281 gaaacattgt taggcaaagg tcgttacaag gattttgatg cagatacatg catcatggct
    41341 cttgatgcgg cagcaaagtt ttcagagaac gatattgctc ccacctatcc ggatgaggtt
    41401 catcgtaaac cggtcgaagc gacattcgac aaaggcaaag taacgacgcc cgaagcttac
    41461 aagagattat ggaagcttta ttgcgaaaac ggttacatgg ctatggctga ccagccggaa
    41521 gtcggcggac agggattccc cgctgtcatt gccgctgccg cagcaaatat gtttttggcc
    41581 tgcaatcagg cttttgttat gtatcccggt ctttcccacg gttgtgcacg attgatggaa
    41641 gatttcttcc agcatccgct gcgcgacatc gttttggaaa aaatgtacac cggcgaatgg
    41701 gcgggaacga tgtgtctgac ggagccgggc gccggttctg acgtcggagc tctgaagaca
    41761 accgccaaaa agaatgctga tggcacctac tccatcgtcg gcaccaagag cttcatttcc
    41821 gccggcgacc atgatctgac cccgaacatc atccatccgg ttctggccag aatcgaaggc
    41881 gaccccaagg gaacaaaggg catttccatt ttcatggttc ccaaaatcag attcaatgaa
    41941 aagggcgaac tcggagaacc caatgatgtg cagtgcggca atatcgaaag caaaatgggt
    42001 atccacggca acgccacctg tacactgaac ttcggttcgg acaataagtg catcggttat
    42061 ctgatgggtg aagaacgtca gggtatgccg atcatgttcc acatgatgaa cgaagaacgt
    42121 cagggcgtcg gtatgatggg cgcttctctg gcaatggccg cttatctgca cgctctggat
    42181 tattccaaac agagaattca gggccaggat gtcatcatga tggccatgaa ggttgaagac
    42241 gcacccgccg tgccgatcat taaacatccg gatgtccgca gaatgctgct caaacagaaa
    42301 gccatttccg aaggtctgcg ccttctggca tatttctgct acttctgcat ggataatgaa
    42361 ttcagcgcaa tgatcgatgg taacgaggag cagaaagaat tctggcatgg cctgatcgaa
    42421 gttcttacgc cgatcgtcaa atcctactgt acagaaaaag gccaggacag catcgaactg
    42481 gccgtgcagt gctacggcgg ttacggtttc tgccgtgaat accccgtcga acagatgatg
    42541 agagataaca agatcaatct gatttatgaa gggaccaacg gtattcaggc tcttgacctg
    42601 ctgggccgca aactgggcat gaagagaggc gcttatttta tgaacctgct gagcgttacc
    42661 aaggaagcta ttgaagaagg caagaaactt gatgctttca agaaggaagc cgagaccgta
    42721 gagaaagccc tgaatgcctg tgctgaaacg gcgctggcct tctccaagat gatcaaaacc
    42781 acgcctttcg ttccgctgat tggcgcctgc gattttctga actgcctggg tgatgcgctt
    42841 gtcggttggc tgcacctcag aatggccctg gttgcgattc ccaagttctt tgcagcagat
    42901 accggtgaaa tggataaagc cttctatcgc ggtaagattg aaggtgcaag attcttcatt
    42961 aaccgcatca ccggactggt cattcctcat ctggaaaacc tgaaacagga tgaacagagt
    43021 gcgatgaaca ttcctgaaga atcatttgct gtctaaaaaa tgatttttta taaatgaatt
    43081 tccggaacgg ggaggcttga agcctccccg tttttttagt gacactcgct aagcgcgaat
    43141 gtaatgacgc gcgaagcgtc agtggaacta tccccggagc gcagctcagc gggatcagac
    43201 actcgctaag cgcgaatgta atgacgcgcg aagcgtcagt ggaactatcc ccnnnnnnnn
    43261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacactcg ctaagcgcga atgtaatgac
    43381 gcgcgaagcg tcagtggaac tatccccgga gcgcagctca gcgggatcag acactcgcta
    43441 agcgcgaata taaggaaacg cgaagtaaag ccgcattatc ctcccccaag cgaaagccgg
    43501 acgggattgt ccccttgacc tgtcttttaa tttatgtcag aaaaagtaat gcgcaaacga
    43561 aaaccgcaaa cgagattaca atcgatcagc gaaatcctgt tcccgattct gaaaaaaaga
    43621 ggaatgattt caaaaatcga ggagaaaaca cttcagacac tctggccgaa agcggtgggt
    43681 cctcaaatcg ccttgcaaac ccggccggat tctctgagga atggaacgct cttcgtcaaa
    43741 acagcctctt cggtctgggt acagcagctt catttcatga aagaagaaat tctcgttaaa
    43801 ttaaataacc tttccgggaa acccctgatc aaagaaatac gttttgtggt cggtcactca
    43861 ccggtaaatg aaaaagaggc taatcctgtt ttgaaggaga agaaagcggt tttaaaaaaa
    43921 agagacagag acatgattgc cgaatgcacg gaagctctga tcgaccagga actggccgcg
    43981 gtattaaaac gcattatgag gctggaaatc agccggcgtc gtcaatggga aagccggaaa
    44041 ggtcgctgaa gacgccggac attttctcgg aatattttcc gggccggtcg taaatgaaca
    44101 tcggcggttc aaccttcaac tcttctttgg aaccttttct cccttcgacc agcacaaatt
    44161 cggccccgga agatacatca gaaaaaatca gtttcatttt tttgggctca atgccgtgta
    44221 acctgaacag gtaaacaagc tccaccagtc gtttagccgg ataaatcgta aaaaccgttc
    44281 ccgccggttt caagagatat tttgccgcgt ttaaaaaatc tttgagggac ccttggattt
    44341 catgacgggc aatggctctt tcttccaggg gattgatccg gccggaatgt aattttcggt
    44401 aaggtgggtt gaataccact gcgtcaaacg tccgttcctg gaaaatagtt ctgacgttgc
    44461 gggcatcgcc ccaaacgatg tcaatgcggt tttccagttg attgagttga acgtttttcc
    44521 gagccatgac cgcaagacgt tcctgaatct ccaagccgac gcattgggtg gtcggaaaac
    44581 gttttgtcag gatcagcagg atgataccgc tgccgcagcc gatatccatg agccgtgtcc
    44641 gctttttcag agaaacgaaa tgggccagca ggatggaatc gatggagaaa cgataccctt
    44701 tttttttctg gaaaatgcgc aaagcgccgt gaagaatttc atcaagggtt tcttcttcca
    44761 tcgttcgaat catcaatctt tttcaatcca gcctgtgcgt gaataatccg ctgcgcagtt
    44821 tgggttcgaa ccatgtcgac ttgggaggca taattgctcc ggcatcggcc acatttatga
    44881 tctgctccat ggtggtggga taaagagaga acgccacggc aaaaccgtcc tgattcacca
    44941 gtttttccag ctcgtccatt cccctgatgc cgccgataaa tttgatccgg tcatccacac
    45001 gcgggtcttt gattcccaga accggataca acagatgctc ctgcagaatg gccgcgtcca
    45061 gactggcgac gggatcgttt ttgtcgtaaa caccttcctt gacggtaagt ttatgccact
    45121 ggcctcccag atacatgccg aaatcatgaa gctggttcgg tgatcggtcc gaaaaatctt
    45181 tgctgacaat gaaagcggaa ccgattttat cgataaattg gtcaggtgtc agaccgttta
    45241 aatccttaac ggcacggtta taatccataa ctctcagttg cgtatcgggg aaaaacaccg
    45301 ccagaaccga ttcgtattcc ccggcgaatc ctctggcgga ttcctttgca cgtcttgtcc
    45361 gggcaatggc ggctgcggcg gccgcccggt ggtgaccgtc ggcgatataa agtgcgtcca
    45421 cggcgagaaa ctcttttttt atttcctcaa tatgacggga gtcggaaata atccagaccg
    45481 tatgcgtcac gccatcatca gaggtaaaat catattccgg catgccgaag gtcacgtgat
    45541 cgacaatcgt attaatgccc ggtctttcac gataactgat gaatacggga ccggtctgcg
    45601 catttacggt gtcgacatga cggatgcggt cttcttcttt gtcttgccgg gtcaattcat
    45661 gctttttgat ttttccggca tcgtattcgg aagcgttcat cacaccgacg attccggttt
    45721 gtgcccgcgt gcccattttc tgcctgtaca cataaaaatg cggcgattca tcctggatca
    45781 aaatgccctt aacacgcata tcggtgaaat tccgtttggc cgtctgatag ataagagggt
    45841 catcgctttt cgttttgtcg gggacgtcga tttccgattt ttccacatgc atgaaactca
    45901 acgggttgcc ggaaaccgct tttttccctt cttcccgggt cattacatcg tacgggagag
    45961 ccgccacctg agcgacaaat tttttatccg gtcgaaccgc tttaaaaggt aatactattg
    46021 ccataatttg atctcctgac agcaaatctt ttcttgcgga atggataaca tactgacata
    46081 atctatacaa cataaaaata tgccggagaa gggcaggaat acgcatggat acgctggttt
    46141 ttattcacgg gctggagagc acggcgcagg gcaccaaggg gcagtttttc tgtaaaaatt
    46201 ttccgcagat gatcattgaa aattatacgg gagattttgc ggcgcgtatg cgaaaacttc
    46261 agagccttct taacgataaa accgatttga tcatcgtcgg ttcgagttat ggagggttaa
    46321 tggccgttca atacgccatc gaaaatgaac accgggtaaa aaagctgatt cttctggcgc
    46381 cggcgttgaa tcttgctgaa ttcacgccgc cttcccgggt tttaaacgtt cctgtcatac
    46441 tctaccatgg ctccgaagac gatatcgttg atccggaaaa ggtcaaagac attgcttccc
    46501 ggtatttcgc tgcgttggaa taccatctgg ttccggacga ccatgttctg catgaaacgt
    46561 ttccgtctct ggattggaag aaactgctga cggccggttg atgatgtcac aaagcacgca
    46621 attttgtcaa atcccacgtc gcccctgcca actatgttgt cgggatagtt cgattttcaa
    46681 atttcactac ttcgctcctg ggataatttc caatctcgat tagtcgggat tggataattc
    46741 ggctgactct tcaatcttcg tcccgacttg tcggggctca atattagaga gtctctctac
    46801 gactagcgct tcccatcgtt agcgggtctc tttaaattgc cttgacaatc catggttgat
    46861 atcttaatct ttcaaaaatt ttattgagtc cgtttactat ggtttctaag aaaatcgtca
    46921 ttcgttacac acccgatacg gttcagcaac cggtaattta tcagctggtg aagcaatata
    46981 atcttgtgtt caatatcctg aaggcgagaa tatttccgcg ccgcgaaggc gttatcgttc
    47041 tggaattaag cggttccaag gaaaatttcg atcacgggat ccgtttttta aaagagttgg
    47101 gcctgaaggt agaaccgctc tccaaaagcg tcagtcaaaa tgccgataag tgcgtccact
    47161 gcggcgcctg cacggctttc tgtccgacgg gagccctgtc gtttgaaaaa aagacgctga
    47221 aggttgtctt tgatccggaa aaatgcaacg gttgtgaact gtgtgtttcg gcctgtccgg
    47281 tccgtgcgat ggaaatcaat cttttataaa taatatgttg acagcctccg ttgaaaaaac
    47341 agcggcaacg gaagtgaaat tttatttttt cgtttctttc cccacctctt tttccacttc
    47401 cttttcagag gcgtcgagta acttgctgac acctccggga gcttcttttg tttttttata
    47461 cagatccttt ccgtctttgt agaagctttt acttttgtct ttgatttcat ttaaaccgga
    47521 ttccaccatg tgaaaagatt cttcaacttt agcttttgtt tccgccggat gctgaacata
    47581 ataataaccg aaagcgacga tcaaaagaag agccgttata aaaatcagct taagcagatt
    47641 tttaaagatt acatgcagaa ataataaaat gatcaagatg gcgcctataa cgacgacggc
    47701 gggatgtgtc gatatatagc cggtgattac atccatgata atgaactctc cttcatcaag
    47761 gggcatatga aatatggtta agtttaccac gaagcctttt gattttacag taaaaacatt
    47821 aaggacataa gtaatcagat gcagtggatg ggcattgtgt tctggccggg gggcgggcaa
    47881 attgcctatt gcgcatattc gatttttacc ttattgaaga tttcagcaaa ggtgcatcat
    47941 gttgggacga accaaaatgt ttacataaaa aataggacat atcgctatgg ataaaagcag
    48001 aagagaggtt ctgaaaaaac tggaagagag acttgattat cgtttccgcg atgtcgatct
    48061 tcttgtaacc gcgctgaccc atcgttctta tgtcaatgag aaccgtcagc cgggcgtgtc
    48121 cgataatgaa cggctggaat ttctgggaga ttccgtttta ggattatgcg tgagtgattt
    48181 gctcatcaaa aaatatgtcg atggttccga aggcgatctg acgaaaatga gatcggttct
    48241 ggtgggcgag aagaatctgg ctcaactcgc acgggggctg caaataggca attgtctttt
    48301 aatcgggcgc ggtgaagaga atgccggcag ccgcgccagg gattctttcc tggccaatgc
    48361 gttcgaggcc gtcgtggctg ccgtttatct ggattccggt tacgagaatg tgcgggcggt
    48421 tgtgagaaaa ttcatcgagc cctttctgga gaaagagact ctaccgtccg cttatttcga
    48481 ttataaaacc ctgctgcagg agttttgcca gagaaaattc aaaacaattc caatgtattt
    48541 gcttgccgat acatccggtc cggagcatgc caaggttttt gaggtaaaag tggtaattgt
    48601 cgacaaactg acggcaatcg gtacagggac gagcaaaaag gaagcggaaa aacaagccgc
    48661 gcaaaaggcc tggaaaatac tgcaggatga cgaaagccag ttatagaaaa ccgaacgata
    48721 actatccgtt gattattccc gtttttatca tgaacagcgg ttgtccgcat cgctgcgtct
    48781 tctgcaatca gaagatcagc gccgggaatt tcccggagaa aataacccag gagtattttg
    48841 acaggaccgt tacctcttat ctggagtgga acaaagacaa gaccagacga gtggaaatcg
    48901 ccttttacgg cggaagcttt accggaatcg attcgggtga tcaggaagca ctgttggcga
    48961 tggctgatgc gtttatccaa cgaggtctgg ttcattccat ccgaatttca accaggccgg
    49021 attatatttt tgaaaaacaa ttgtccatgt tgaaaaaata taacgttgcc accgtagaga
    49081 tcggtgcgga atcttttgtt gatgatgtgt tgcgctatgc cggaagaggc cactgcgcgg
    49141 cagatacgga aaaggccgtt atgatgttga aaaaatacgg attcaaaacc gggcttcatc
    49201 tcatggccgg tctgccgggt gacacggctg aaggctttga ttattctctg gacagaacca
    49261 tcgaactttc tcccgacacc gcaaggatac atcccgttct tgtctttgca ggaaccgccc
    49321 tggctgagga attccgaaag ggcgcgtacc ggcctctgga cattgcctct gccgtggagt
    49381 tatgccgcag agcctgggaa aaactagccg ctgccggtat ccgcatcatt agaatgggcg
    49441 tgcagaccac acccgagatg gaaaaacaag gaacggttct ggccggaccg gtgcatccgg
    49501 ccttcggaag cctggtgcgg tcctctgtat tttatcacag caccgttaat gttctgaaaa
    49561 agatttccgg ggatgtccag gaaatacgtt tttatttatc ggagcgggat ctgtccattt
    49621 ttcgaggatt aaacaatatc aatacggagg cgataaaaaa gctttacccg cgtgcgaaat
    49681 taattataga atcacagtcc gaccggccgc ggggtgaact ctccgttgcc gtaaattcgg
    49741 gaccgttgtt gcattggacg attccgggat ttcatcaatt ggtgagaggg caataaagat
    49801 catgtttaaa tcgggattca tcggcatcat cggacgtccg aatgcgggca aatcaacgct
    49861 tttcaacgcc attatcggtg ataaaatatc cattatgacc cataagccgc agacaacgag
    49921 aaataaaata accgggataa aaaattatcc ggatgcccag ttgatttttc tcgatacgcc
    49981 cggaatgcat aaaccgaaaa caccgctgaa ccgtgccatg gtgcggacga ccagagaggc
    50041 aatcggcagc gttgatgtcc tcttgatgat gattgaagcc cagaccgatg tccagcccca
    50101 tgatcttttc ctgatagaat ccttgaacga gaccaaagcg ccggtgctac tggtaatcaa
    50161 taaaatcgat ttgattgaaa aaaagtttct gctgccgcta atggataagt ttcgaaggat
    50221 ttacgatttc cgggacatcg tcccgatatc cgcattgcac ggcaacggcg ttgaagaatt
    50281 gctgacgatt gtcaaggaga tgcttccgga gggaccgaaa tattatccgg acgatattgt
    50341 gacggatgcg accgagcgat ttattgcttc agaatttatc cgggaaaaaa ttacgcttca
    50401 taccaaacag gagcttcctt attccgcagc cgtggaaatt gatttgttca aggaggatga
    50461 agcgaagaat cttatccgga tcactgcgac gatcaatgtg gaaaaggatt cgcagaaagc
    50521 gatcatgatc ggcaaaaaag gcgccatgct gaagaaaatc ggcacccagg cgagactgga
    50581 tatggaaaat ctcttcggtg ccaaagtgta tctggagttg tttgtcaggg taagaaaaga
    50641 atggaccgct tcggaaagaa tgcttgagga attcggttta ctgaaacatt agaggctgta
    50701 tgaaaatcaa accggtcatc gcgattgtcg gacgtcccaa tgtcggcaaa tccaccctgt
    50761 tcaacagaat agccggaaaa caaaaggcca tcgtgatcga cgagccgggg gcgacacgcg
    50821 accgtaatta catggattgt gcatatcacg acagggcctt tacgctgatt gataccggag
    50881 gatttgaacc ggcctccgaa gaaaaaattc ttgtccagat gcgcgagcag agcaatctgg
    50941 ccattgaaga ggcagacgtc atcattttta tgatggatgg aaaagaaggt cttacgacgt
    51001 ctgatatgga aatcgcccgg cagttgcgag ggaaggggaa aaaggttttt tatgtcgcca
    51061 acaaagttga cggggaacgt tacaaggaaa tgctctccga tttctaccgt ctgggaatcg
    51121 atgaggttta tcctgtttca gcgcagcaca acctcggtgt ggacgaactg atgaatgcga
    51181 tggtgtctga ttttcctgaa tcgtccgaag aaaaaagtaa aaatgacgac cagattaagg
    51241 ttgccattgt tggcaagccc aatgtgggga aatcatcgct ggtgaacaaa atactgggca
    51301 aggaaagaac cattgccaac ccgattgccg gaaccacacg cgatgccatc gattcaaccg
    51361 tcaagattca cgggaaaaaa tacctgatca tcgatacagc aggcattcgc cgaaagagta
    51421 aaatcagtca gacactggaa aaatacagtg tggttcaggc gctcaaatcc atcaatcggt
    51481 gcgacatcgt gcttgtgctg atcgacgccg aagaaggcat taccgaacag gacacaaaaa
    51541 ttacggggct ggcctacgaa cgcgggaaag cctgcctgat cgtcgtcaat aaatgggata
    51601 aaataaagaa ggataatgca accgttggaa aatacgtcga agaaatcaag gataaactca
    51661 aattcctgga ttttgcaccg attattttta tttcggcggt aaccggacag cgtgcgccga
    51721 agattttcga tctgattgat aaggtgtatg cccaatatac gcaaagaatc ggcacatcgc
    51781 ctttaaataa tctggtcgag caggccgttc gaaaaaatcc tctcccgcgc tatcagaaca
    51841 ggctgatgca aatttattac gcaacccaga ccgatattaa gccgcccacg tttgtcttct
    51901 ttgtcagtaa cagcaaagga atccatttct cctacgagcg ctatctgatg aaccagattc
    51961 gtgaaaaatt cgaatttgac agcgtgccgc tgaagcttat tttccgaaaa aaaagttaga
    52021 gggattccgg atttcgcgct taatgatatt attttaagca aatgatcatc ggcggcattt
    52081 aaaattctcc tgcaccttga acggtttttt gcgcatatgg ttgcgaaggg cttcccggcg
    52141 ttagaaagaa tgaattcaac gtggtctttc cagaggcaag tgaaatattt ttgacaatca
    52201 tcaatacgct ttagattttt tatccgaatt gtgatatgaa atgttcgtaa aattcattat
    52261 ggagttgcat aaaaacgatt tgattattgg tcattcaaaa attattttct aagagggaat
    52321 cgttatgaaa acatgtcttc attgcggaaa gcaactgcgg gatgaaacga cgaaatgttt
    52381 tttctgtggc caaatggcgg atgaattgcc gcaacagaaa aaaggttttg agatgtcgaa
    52441 aaagaacagg gatatcgctc tattaattat cgtcctaatt atctgtctcg ttatttatat
    52501 attaaaaaaa taattattta agaatgttgt attagaacga tttgcgattn nnnnnnnnnn
    52561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcgttcgcct ggacatatca ggcgccgtac
    52681 cggagaatgc cagaacctgc ctgatcgtat ggtcctgcat gggcggcgca gtgcaaatgt
    52741 atcgcgtacc ggtggaaaaa tttacagatc agggatgcgc ctgtattctt tataatccgc
    52801 gcggccacgg tcgttccgaa ggagaactgg aaattcaaac agctttaaat gacctggatg
    52861 agattcttca ggagaatgtt ccttccgcaa ttccgctggt gatcgtcggc cacagtgccg
    52921 gagcgaacgc cagtctgcaa ttcggagtcc gctgtcttcc gccgaagcaa ttcgtgctga
    52981 ttgcgccggt tttggattca cgtgaatccc tgttttacat gtatcaaaga aaaaccattg
    53041 atgaattcgt cagtattctt tgcgcctatg tacaggatga cactctggta aggaaagtgc
    53101 ttgtcgatga taaatggctg gatgccgatt tatggaaagc ccgtcattac acacatctgt
    53161 tggatcatgt tcatgcgcgt atgcgcatag gcacattcct ggaaagactc tttatccccg
    53221 gacacaatgc ctttgctcaa ctggaaaggt tttccgcgca gacgcgcatc tttatggccg
    53281 gggaggacac ctggtatccg gccgcgacga cccgtcgttt agcggacaag tacggtatcc
    53341 ctgttgaaac actgccggaa gcgcaaaacc atttttttac cggtgcctgg gacagtatgt
    53401 ggacaaaaat tcttaatctg atccttttac ccgggtagtt ttctttcttt tcagcgatgt
    53461 ttccctgata caaatcataa ggggcatcaa acaagccggc gtcctgcaaa cgattttaag
    53521 cgcaaccgct cacttgattc gtcaacgttt gtggtcattt ctatgaatgt cgttccttca
    53581 ggaaacagtc tgcgcgaagg attactcttg tagttttttg ataaatttgt catataaccc
    53641 tcaacttagg gagggtatat ttattgattt atttccgcaa catcagtctt tcgtttctgg
    53701 ataaaaaact gttcgacaag atgaactgga ccatccaccc caaaagccgc attggactgg
    53761 tcggtgaaaa cggaaccggt aaaacaacac tgtttagaat gatcatggat gacgtgcgcc
    53821 ctgacgacgg cacgctggat attcccggaa aaaaacagaa gatattcggc tatctgccgc
    53881 aggacctggt ggaactggaa tcgattcctc tgattgatta tctcaagcaa aaaagcggca
    53941 tggccgacct ggaaacgtcc atcaaagatc tggagcagaa gttatccaaa atcaagcatg
    54001 acacagccga atatcgcgag ctttcgcaaa aatattccga tgcgacagca ttattccatg
    54061 ccagggacgg ttacgctttt gaatccagag ccaagcagat gctcaagggg ttcggcttta
    54121 aagaaagtga ttttaaacaa aattgcgatt cgttttccgg tggctggaag atgcgcattt
    54181 tgttgacatc gattcttctg gcaaaaccgg atatcatgct gttggacgaa ccgacgaacc
    54241 atctggatac ggaaagcatg gagtggttgg aatcgtatct caaggatttc cgggggacca
    54301 tgattgtcat ttctcacgat catttctttt tggataaagt cacgacgcag atcgcggagt
    54361 tgtcctcctg cgctattcac ctttacaagg ggaattacac gcactatctg acggaaaaag
    54421 aaaagcggca ggacgctttg aaaaaggaaa tggaactgca aaaatcgcaa atcaaaaaaa
    54481 tagaagagtt tgtggagcgg ttccgctaca aggcgtcaaa agcggcccag gtgcaaagcc
    54541 ggattaaaat gctggaaaag ttttcggagg taaccccgga gggaaaaaca aagaatgtgg
    54601 ccatgaaatt ccctgaaggg aagaaaagcg ccaaagaagt cgtcacggta accggcctgg
    54661 gacaccgcta cggcgatttg acggtgtttg gcgatttgaa ctttgcgcta ttccgcggcg
    54721 ataaagtggc ctttgtcggc atgaacggct caggaaaatc aaccttatcc agattacttg
    54781 gcaaaatgga gaacccttcc agcggcgtgg ctaaatacgg cgacggtgtg aacatggctt
    54841 tttattctca ggaaagttcc cagaacctga attacgaaaa aaccattctg gaagaaatca
    54901 atgccgttcc gaccgatgcg tcggaccagg aaaaacgcaa tctgctgggc acttttttat
    54961 tttccggaga tgatatcttc aaaccggtat ccgtgctttc cggcggggaa aagtcaagac
    55021 ttgcccttct aaaaatcatg ttgttggata ccaatttcct gattcttgac gaaccgacca
    55081 accatctgga tatcaaaaca aaggacattt ttcaaaacgc tctgatccat taccgcggga
    55141 ccgtggcgat cgtgtcgcat gaccggtatt tcctggatca tctggtcaat aaggtgtttg
    55201 aactgaagga cggagcaatc cattactaca acggcaatta ttcttatttt atcgagcagc
    55261 gcagccggcc ggcggatgcc gcaggcgatg agaagcctgc gcaaggcgtg atcagaaatc
    55321 agatcgtaaa cgccaaagag aaaaaacgtc tggaagcgga agaaagaaac aaaacgtata
    55381 agatcagagc ggcgctcaaa aaggaactgt ccgatgtgga gaaaaaaata tccggactgg
    55441 aagaccggaa aaccagacag gaaacggcat tatgtgatcc gcaaactaag aaagatgccc
    55501 ggacaataaa atcgatcaac ctggaattaa aagatatcaa tcgcgacctg gaacaatcct
    55561 atacgatctg gacggatttg catatgaagc tggaacaaat atccgcgaac gctgagcctt
    55621 tttcctctta accgccgtca agagatgctt tctccatatt ctgataccag gacgccggat
    55681 cggtcagaaa ttcctctgtg tcacgtaacg aaagaaagtg ctgtctttcg atgctggcga
    55741 gcagattgaa aaaagctttt tctttgggat catcgcttcc ctgctccagt ttggtgtaga
    55801 gagccatgcc tctggcctca aagtcaatag cagtccgtat ggctttcagg tcatcttcgt
    55861 tgcctttgat acgcgcccct tttttctccg acatcgcttt gataatatta ccggcaacag
    55921 attttttgac tttcagaggg atggtcgccg gccatttttc cttcgcctcc catttttcga
    55981 agagtttttt cagcatatca tagtgttctt tttcttcgtc ggcaatctgt ttaaacatgt
    56041 ttttcccggc cagatttttt gttcgacggg cgttagccag ataaaaatct ctttcatgta
    56101 actcattgtc cagggcgaat tttaaagcgc tgattctctt attcatcttg atacctcctc
    56161 gagcggatgt ccaagtaaac agtccctcca cgacatatta tatgagatat tgcgcatgag
    56221 ggcaataggt ttgtttttta aaggtcgggt actcgggatc tagtcctctt tcacgtcaag
    56281 catatattcg gcgatgttgt tgcaaagatc agaaatgcgt tcgaggtgca aaagcagttc
    56341 aacaaaaatg ggcccggctt ccggatcgca aaggcgtttg tggaatctct ccaaatgatt
    56401 gtcccgggct tctttgactt tgatatccac ctgttcctca cgggcaatga cgtccatgat
    56461 ggttttatcc agcggcttat cgataacgga aaaagcgtcg ttaaaattat cactgaccag
    56521 gtcgatgatt tcatgaagtt cgacttctcc gcagtcgctg aatttgatat ttttaccggt
    56581 tttttgagcg gtaagcttgc tgatgcataa aacgtgattg ccgatacttt caatatcact
    56641 ggtcatggcg gtatatgcaa aaacgttttt agaataatat tcagaaagat cctgtacgga
    56701 taatttccag agataaccga cgatttgttc ccggatattc ttcaccacca tggcgatata
    56761 aaaaagggat ttatcgtccg acggactgta atcgttaatc attctcatgc tctttaaata
    56821 catgtcgcga accatgttgg tttgacgctt cagttctttg tggacgttat ccagtgcttt
    56881 ctgcggattg gtaatgtcct gaacgtcgag atattccggc cagacaggga gaatgtcgtc
    56941 cttgccgggg aagaaccggg tcatcatggc ggcaaacggg gacagcagaa aaatgaaaat
    57001 cacgacaata aacaagttta acagaaaatg ggcaagggcg atctgctgcg cgacgcttga
    57061 cgaaatatgt tgcaggatcc agacaaaaga gggcattgcc atgaaacaca atccgatccc
    57121 ggttgattta aaaatcaagt gagaaaaagc cgttcgtttt ccgctgcgcg tagcgaccgt
    57181 tccggccagc agggcggtta tcgtagttcc gatgttggct cccatgacca cagccacggc
    57241 attttccagc gagacaatat cctgctgagc caaaagaacc atgatactga tcgggatggc
    57301 cgatgcctgg acaatgccgg tgacggcaat tcccataccg atgccgataa gcggattctg
    57361 tgttttcgta aacagctcaa tgagcagagg ggattgcttg agcggttcca cggccttgtt
    57421 gaccagatcc agaccaaaga acatcagacc gaaataaaag atgatctctc cgatcgtctc
    57481 ccatttattc ctgccgatca gccacaataa tccgcccaag gaaatgatca gcggcgatat
    57541 atccgtgaag cgccagatga ccagttggac ggtcaatgtt gtgccgatat ccgcgcccaa
    57601 aataatggcc agagaattgg agaagctgat taacccggcg ctgaccagtc cgatggtcag
    57661 gacagtcgat gcggaggagc tttgaaaagc aattgttgaa acgatgcccg tcagcaatcc
    57721 ggaaatcggc ctgccaacgg catattttat gaatttttta atacggacat cgatcaattt
    57781 ccttatggag gaggaaagat tgaccatgcc gatcagaaat aagatgacgc cactgacgat
    57841 gataaaaatg tcttttattg ccaaatttta ctcctggttg aatttaaatt tccgtttggc
    57901 gccgggttgt catttttttt catatatccg tcaaacgatt tgaacagttt ctcttttgca
    57961 cagcatcgtt aacaaaatga aaagatcgtg ttgcatcttt tgcgtttttg acaagatcgt
    58021 tttcttgaat aacgttattg tgttgatgaa tgcatattga caaatttatc ttaaaaagac
    58081 aagggcaatg atgagtccat aagattgttc agaaagaacg gagcatgatt tttattcgga
    58141 tcggctgtga tgcgttatca ttctgatgcg acgcgatgct tcctgatgaa gaaaatctgt
    58201 tttatgcggg attgtactgt tgactgaaaa ttggatggcg tgccgctgcc agattgacga
    58261 tgcggcttcc catttgtccg taggagtaac cgatttctgc tgcagcatgg gtcatcgacg
    58321 tttgcggatt gatatcggga ttcgggttga catcaataac gtaaaatatt ccgtttaaat
    58381 aacggacgtc aatgcgtccg taatcgcgac accctgtggc ccggtaggtg gcaatcacca
    58441 cattttcgag ttgggccctc gccgacgtct ccagctccgc cggtacgcgc atatcgatgc
    58501 tttcataaag acgcgacccg gcaacgtatt tggcctcata ggaaaaaaga cggtcccgca
    58561 gatccgtcag ctcgctgtaa tccatttcca cggcgggtaa catggaaatg tgtccattcc
    58621 cccagacggc attaagaaat tcgcggccgt taataaattg ttcaacaata gccggttgtt
    58681 gatatttatc cagaataaac gccacacggt tttccagttc tttcggagtc gtgacgaccg
    58741 attccggaga gatcccgatg ctgcaatgtt cattagccgc tttgacaatg gacggaaaaa
    58801 tattccattc cccgacatcg gtgttatcaa aaaccgccca tggcggcgtc ggaatattgg
    58861 cgtcgtccag gactcgcttg atttccggct tgtcatgcgc cagtgccagc acgtcgcggg
    58921 aggaaccggt gaagacaaag cccatacgtt ccatgaccgc ggcaacatcg ggctcactgt
    58981 gcggcacgcc gggaagttcc tcgcaccagt tgaacacgac ataatcccgc ggattgaagt
    59041 tatggagctg ttgggccaga cgatcatccc ggaccgtgac gatttcgagg ggatgtccca
    59101 gatccttcag ggcattgccg agtgttgcca cgtcctgttc ggtttcaaga gtttcttcag
    59161 atgtccattc gggattcatg ttgtgcagca gaagaaccgg tatgtcgtaa cagagtttgt
    59221 tcataacacg cctgtctgaa cgtccggcta aacgactttt gcctgttgat gccattttac
    59281 cgggcgaaat cgtttttgtg atgcaaaatg tttacgaccg tttttactat aacagatttt
    59341 atccaaacag ttcaaggaaa aatgaatctt atccgttcag atattttaaa cggtggtgaa
    59401 atatgcttaa gggattgaca gccggctttt atttgcatat agtaattata caacggattt
    59461 gaaaaagcat ttaaccttat ttttatccaa acataataac gaagcaatga acttcaggga
    59521 gatggatcat catggcagat cgcacgatat acattacaaa attcgatatg gatcgtttac
    59581 tggaattaat tgacggcgtg cggtcaacgc ccaggcacaa caaagccaat atcgacctct
    59641 tggaaaagga attatacaac ggtacagtgg ttgattctga ggatgtgccg gcggatgtga
    59701 tcacgatgaa ttctcgggtc agcgttacgg acattgaatc cggagaacaa atgacctata
    59761 cccttgtttt tccatccgaa gccaatattg cggaaaacaa actgtccatt ctggttccgc
    59821 tgggaatggc tctcataggg tatcgtaaag gggatatcat cgaatggcct gtgccgtccg
    59881 gcgtcaggaa aataaaggtc aatgatgtta tttatcagcc ggaggctgcg gggaattata
    59941 atttataaac agagaacctt cttgccccgg ggcaaagggt gattcttttc tcgagaaaac
    60001 tacgcaccat actgctcttt gatccaattc aggtaagcac cggttttgat gttttcaacc
    60061 catttctttt ggtcgaggta ccagttaacg gttttttgca gaccggagga aaacgattct
    60121 ttcggcgccc agttcagttc cttttgcagc ttagaaaaat caatggcata acggcggtcg
    60181 tgtccggggc gatccttcac aaaggtaatc aaccggcgcc tcgggctatt gtcggacgcc
    60241 ggttgtatgt catccagaat atcgcagatc aactgcacaa tggcaatatt ttccatttcc
    60301 gccttgccgc cgacattgta cgtttcacca tgccggccgg ctgtcatgat ggtccagatg
    60361 gcctcacagt ggtcctcgac ataaagccag tcgcggacat ttttaccgtc accgtaaacg
    60421 ggaagcggtt tgccttccag ggcgttgaga acgatcaggg ggatgagctt ttccggaaac
    60481 tgatagggac cgtaattatt ggaacagttg gaaatggtca cgggcaatcc gaacgtctta
    60541 tagtaagccc gcaccagatg atcggaagcg gctttggatg cggaataggg gctgttcgga
    60601 cgataaggcg tgtcttcacg gaaaaggccg tccgctccaa gactgccgta gacctcgtcg
    60661 gtactgacgt ggtgaaaaat cttgaaccgg tcgccgcgcg ctttggcgca ctccagaaga
    60721 ttgaaggttc cgacgatgtt ggtctgaatg aaatctccgg gcgttttgat ggaacgatcc
    60781 acatgcgatt cggctgcaaa gtggcaaacg ccgtcaatat cgtatcggga aaagatttct
    60841 tccagacggg ccctgtcgca tatatccgct ttttgaaaaa tgtatcgtga tgcaccgttt
    60901 gcggcaatct ctgcaagatt ctcaggattc cccgcgtaag ttaatttgtc gacattgata
    60961 attcttcctg aatgatgaat ccggtcgatt aaataccgga tgaaattgct gccgataaaa
    61021 ccgcagcctc ccgtaaccag taagttatgc aatgtgacag ggggatggat gttttttgtc
    61081 attcggattt actcttgttg ggactgatca atttttttat ttcaggaagc cctgaataat
    61141 tctgttgatg gcttgatcgt aagtcagtcc caggctcatg agtttcgtca actggtcatt
    61201 ggcaattttg ccgatggagg cctcatgcgt cagttccgcg tccggatgca gggccttcag
    61261 tgcgggcatg gtttcgttgg tgccgttgtc catgataata gcatcgcatt cgatgtgtcc
    61321 gaagcatttg ttcctggctt ccatgttcgc gtgaaagatt tgcttggaat tgcctttcat
    61381 caccgagcgg gaaatcatat tggccctgct gccgaagcct ttcagtatga tgttattggt
    61441 ggatacagcc gattgttcgc cttccgtcag gacgcgttcc ataatcagta acagactgtc
    61501 cggaccgagt tccgcttcat taagacggtt ggcttcatcc acaccgctta tctgcgtcag
    61561 ctccatttcc gctgttgctc cctcatccag aaagactttt gttgttggat taagcgatcg
    61621 tcttcctttt ccctctccca tggcgaaatg tttttccaca taggtgactt ttgccttctt
    61681 tcctacatga aaatcatgga tgcccgcatg gccttcggct tcggacgaac cgcagtgaat
    61741 gccgcaaccg gccacgatcg ttacctctgc tccttcgccg atgatgaatg aattataaac
    61801 aacatcataa agtccggcct ggctcaaaat gaccgggata tgaacggatt cgtttttggt
    61861 ccccggttta atggtcacaa taatacccgt tccgtcggcg ttggtttcaa tattaatatt
    61921 ggccgagact tcgcgatgca gcagtttacc gtttttcctg atattgtagg cgcctttagg
    61981 caggctttcc agatcggcaa cggtcttcag cagtttttta tctaaagcat tcagcatttt
    62041 caaatccctc taatttgttg tggtatgcgc aaatactcat gtcattcagc aacggcagcg
    62101 catctttcag attgccggta taggcgatgc gtccctgatt taaaatgatg atcacgtctg
    62161 cgacatccag aaaagccttg ttgtggctga caacgatcgt tgttgtttta ggcgtcagtt
    62221 cttttttgag aagactgacc atgggaccga tggtccataa atcaatgccc gtatccggtt
    62281 cgtcataaat agccagcttc ggattggtgg caatcgtggt ggccagctcg attttcttga
    62341 tttcgccgcc cgaaagtttt gaatcgactt ctttatcgag aaaagaaagc ggacaaatgc
    62401 ccacccggga tacaatttcc agaaactttt tctcctcatc cgttcccgtg gcgatcgaca
    62461 gtaaatcacg gaacgaaatg cctttaaaac gggccggctg ttgaaaacta tatgcgatgc
    62521 ccagtttggc tctgtccgta atcgaaagac cggagatatc ctggccgtta aaaacaatgg
    62581 agccttgtgt gatcggatta atgcccatga tcagtttggc cagcgttgtc tttccgctgc
    62641 cgttggggcc cgtaatggcg taaaatttgc cgtcttcaaa ggtaaaatcc aggccgtcaa
    62701 tgatattgcg tggggcactg ttttcaccat cgccgccggg agcggtaaaa aaaaccttat
    62761 ttaatgttaa cattcgttga acccgccctt atcttaaaat attaaaaaat atatgaacac
    62821 catcaactgt tgaaaatatc acaattttcg gtggcactat agacacaatt tcgtctaaaa
    62881 agcaacgctg aaaaatgata catccctgcc ggtatttttc aggagtggaa ttgaagaaac
    62941 atgggtgatg ggccaaataa aatcaggcgg cagatgtttt gaaaacaaat gccgcctgat
    63001 ttttctttcg aggcacaacg acaaaaagca ttttcctttt tcaggggaaa attattgcac
    63061 cgttatgccc ccttcgtcaa aaaaagttta aatgaaattt tctttatttc ttctttgctg
    63121 ctttctttgc cacttttttc ttgggagctg ctttctttac agctttcttt gccacttttt
    63181 tcttgggagc cgctttcttt gccgctttct ttgctacttt tttcttcggt gctgctttct
    63241 ttgctgttgc cattaggtga agcctccttt ataaaaattt gatttcaact gctgtaaaaa
    63301 actttacttg cttcgttgcg aagaatttta cttcattttt tttcttacgt caacaaaaac
    63361 tataaaaaat aatgaattat tttacaagca tcccgcaagc gaaaagatcg caggagaaat
    63421 gccggggaat gaaaaacgaa aaatggcgat gttttcatga agagcgatac gctgcaggcc
    63481 acgtcatcac tgggattccc gatgatgaag aaaataaaat atagtttagt gcaatcgccg
    63541 catacaggca ttccacgtgg aaactctttt ttttaaaaaa atatttctga ggactgaaaa
    63601 aaataaaatg agaaaaatat tcaaatcgac aaaaaaaatt atggtgaaaa cgaaaagtat
    63661 ggaggagaaa aattataaat aggttctcgc tccgacatat tgtctggtga agtaatcgga
    63721 cgaaaggtgg tcaatgcgaa tttcattgcc ctgcgatgag gcatgaatga actggttgtc
    63781 gccgacatat atgccgacat gcgaaacatg atgattcccc cgatcggcaa aaaagaccag
    63841 atctcccttc tgcagttcgt ttttctcaat ggcatttccg accgcatact gttttctgga
    63901 gttgcggggc agattcaggc cgttgagctg atacaccgtc atggtcaagc cgctgcaatc
    63961 gaatccgtca tcgggagagg ccccgcccca gagataggga acaccgagaa aatcccgggc
    64021 tgttgtgaca atggattccc tgagatagtc ggttccgtat tgtttttgtc gggctacgga
    64081 ataatcttcg ggacggacaa tgtaaaattc ctcaatgacc tttgcgtcga tcagttccag
    64141 ggctctcttt ttagccaggt ccttggtgct gaaattaccg aagcgcactt tgaagaattt
    64201 gtcatcggca agaaaatagg ttgcatcgag cccgtgattg attttcagcc gttccgtcaa
    64261 gcgaactgca ttttcaacat ttcgaaaagc gcccgcctgt atcgtatagc ccatcagatt
    64321 taaagcaggt ttgtatctgg gcgccgacgc aactctccgg tgatgcgcct tgcccgagca
    64381 ggccagcatc agggtaaaaa gtaaaaacaa gcaaatgatc agccggtatt ttttcatgat
    64441 tctatattcc atctgttacg cacataattt attgcctgtt cggcggactg gattcgtcct
    64501 tccagctgtt cttcctccac cgcaagaaga atatctttga tgattttccc cggagtaaac
    64561 cccatggcga tcaaatcgtt tccggttaat aaacgaggag gatgcagatc gtcaagcgct
    64621 agaatctgca actgttcgcg gcagaactca taattgtcca gcattccgtg actggccaga
    64681 caatcgagac gatgcagttc cagatgaaga tcgaaatcgg gcatgcgcag aaatctttta
    64741 agacgtccgg gccgcatttt ccggacattc atgaatacca tatgctgacg aatcagattg
    64801 acgacggttt ctctctgcat gcgggaaaat tttaatcgtt gcatgatggt gtcggcaata
    64861 atttctccct gctgcacatg gccgtagaag tgcacgccct tttcgtcttc gctgcgggtg
    64921 agcgctttac cgacatcgtg taagagcgtt ccccacgcca ggcagatatt tttttcccgc
    64981 tctccgtctg cgggcagcaa ctcaagcatt tttaacgtgt gctgccagac atcgccctcg
    65041 ggatgaaacc gcggcggttg ttccacgcct ttgagacggc agatctccgg taaaatgttt
    65101 tgcaggatat ccgtttgcat catcagttca aatccccggc gggcgccgcc gcgggtcaga
    65161 atcttgttga gctcttcccg gatgcgttca acactgatgt gtcggatatt cgaagcttcg
    65221 cgtttgacgg catcctgtgt cgccggttcc aggtcgaaat gaaggttggc tgcaaaccgc
    65281 accgcccgga gcaacctcag ataatcctca cggaaccgtt ttcccggatt gccgatagtt
    65341 ctgacaattt tattgtcgat atcaaaacgg ccgttgacat aatcaataat atctccggta
    65401 tccggatcca ttaaaaggcc gttgatcgta aaatcgcgcc ggaaaacatc ttcccgggca
    65461 gtggaatagt gaatccggga agggtgccgg ccgtcttcgt aaacatcatc gctgcgaaac
    65521 gtggcaacct caaaaggatg attgtcgata atgacggcaa caacgccgaa cttggcgccg
    65581 atggctacgg tgcggggaaa gagtttcatg acctcctccg gccgcgccga cgttacaata
    65641 tcgtaatcgc tggaagtctg gttgagaata aaatcgcgaa cacacccgcc gacaaaatat
    65701 gcctcgtgac cggattctct cagccggtga ataatttccc tggcatgaag gcgatgggca
    65761 tgatcaggca ataatttatc ctctggtgat tatctcttac ctaattttat cgcacagaat
    65821 tcgccgcaca tcgtgcagcc ttcatttttg gcgcttttgc ttttttccag aagcgaaacg
    65881 gttttttcag gatcgatgga caaatccacc tgtccctgcc agtcgaaatt tttccggcac
    65941 gaagacatct tccggtcttt ttcacgcgcc tgcttcacgc ccttggcaat atcggcaatg
    66001 tgtgcggcga tacgcgcggc aataacgccg tcgcgaacat cgttcaaagt cggcagccgc
    66061 agatgctccg caggcgtcac gtagcacaga aaatcggcgc cggcggcgcc ggcaatcgct
    66121 ccgccgatgg ctgacgtaat gtgatcgtag ccgggtgcga tatccgtggg cagagggccc
    66181 agcacgtaaa aaggagcgtt ctgacaaata tctttttgca gtctgatgtt ggtgacgatg
    66241 tccgtcaggg gaacatggcc gggtccttca atcatcacct gcacgccagc ggcgcgcgcg
    66301 cgtgcggcaa gcgccccgag gatgattaac tcctgaagtt gtgcctgatc ggtggcgtcg
    66361 tcaatagctc cgggcctcag accgtcgccg aggctcaaca ccatatcgta acgatgagca
    66421 atttccagca gccggtcgta ttcctcataa agcgggtttt cttttttatt gcagtccatc
    66481 cagttggccg tcatcgaacc gccgcggctg acgatgccca ggattctttt ttgtgatttc
    66541 agcgccgaga cgctcaaccg ggtgacgccg cagtggaccg taataaaatc cacgccgtct
    66601 ctgccgtttt cttcaatgac gtcaaaaata ttatccgctg tcatttccga tatggctttc
    66661 ctgtcctgca gcattttgac tgccgcctga taaatcggga cggtcccgat tgccaccgtt
    66721 gattttttca tgactttttt tctgatggcg gccagattgc cgccggtgga taaatccata
    66781 accgcatcgg cgcccgcatc aacggcaatc ttcagctttt tcaattccag atttaaatcg
    66841 gtatggtctt tggaggtgcc gatattggcg ttgactttcg tccgcagacc ccggccgatg
    66901 gccagcggtt taatggacgg atgttttaca ttgcggcaaa ggacaatggt gccttcggca
    66961 attcctttgc ggatatattc gggagcaact ttttcttctt ttgccgctgc cttcattgct
    67021 tcagtaatga tgcctttacg ggctttttct aattgtgtca tcgtatcctc cgaaatgtga
    67081 aagcgatttt atatcgccaa atattaacta aatttttcaa aaacattgac cgatccgtaa
    67141 ccgtgtccca aaacaaccgc cttccggata gcgtcggcta ccgtgatttt agcctgcaac
    67201 acagcgtctg caagactttt cctgcgggcg agaccggacg ccagccccga agcgaaggtg
    67261 cagccggtgc cgtgcgtatg acaggaacgg attctggcag aggaaaattc gcagtagccg
    67321 gatccgctgt acagaatgtc ggtcacatcg gcatgtttgc ggtcgggcag atgtccgcct
    67381 tttatgacga catttgccgc gccggattcg taaatgaccg cggcagcgtc cttcatgtcg
    67441 gccatggttt taattttatt tttcgagagc gtttccgctt ccgggatgtt cggcgtaatg
    67501 accagcgcca gcgggagaag cgtctgcacc agcatctgtc gggctttgcc ggtcatcaga
    67561 gttctgccgc ctttggcaat catcacggga tccaccacaa cgtttttcag tttatatttt
    67621 ttaatttttt taacgaccac ctgaataatc tcttcggtcg gaagcatgcc ggtcttgatc
    67681 gcgtcgactc cgatatcgct cagcacgaca tccatctgct ttgcgacaaa tgccggatca
    67741 accggataaa ttcccgacac cccttttgtg ttttgtgccg taacggccgt gactacagtc
    67801 aatccccagc agccgcaggc gctgacagct ttgagatccg cctgaattcc ggcgccgccg
    67861 ctggagtcgg aaccgccgat gcataagact ttgcgcaatt tcatgattaa accgcctccg
    67921 ggaaacgatc cttgttttgg tacttaataa caagaagctt gccgtttgca aaccggatac
    67981 acgcatcatt gtccctgatc cggttgctga taccgcggga ttcccccatt ttcaaatttt
    68041 cattttccag aggatacaga aatccggaaa gggtgattcc ggtaacctcc ggactcagcg
    68101 atagcaggga tacggttttc cctgtgtcgc cggtaaaatg cgtttcgtta tccgccagaa
    68161 atatttcgca gaacgggtcg atcagcaccg tttgaattcc tctttcccgc cctttaaaaa
    68221 gaaggaaaat attggacagc gtatgatcca tcctgcctcc tagagcgcac cagatatata
    68281 ttttttccgg ttttacctcc aacgcgtaat ccagggccag ttccgtatcg gtaaagtctt
    68341 tgtttgccgg atattttatg attttaatgt tttccgaatc ataataagcc agaagagcgg
    68401 cgtcgatcga atccatgtcc ccgatgatga cgtccggttt gatgccccag cttttgagat
    68461 gacgggatcc gccgtcgcag ccgatgatca gacaatcatc ccgcttgccg gcctggctgt
    68521 taaaaaatgc ggcatcggtc gattcgccgc cgctgactat gattactttt ccgcgcacgt
    68581 gatttacctg aatggcgaca cccgtttaag gatatccggc tttataaatt aagtcattcg
    68641 tatacttgta gaaaatttat tatattcaac tttaaaaagc aaattgtttg atcctctgat
    68701 gtaaaatcgt ttttggatac cgcttttcag gaccatcatg aaaaacgtca caaattaagt
    68761 cttgacaaaa gcaccgttgt ttgtttttta acaccctgta aatattatag tttaaaaaaa
    68821 taaaaaataa aatcacttct aaaaacaaca gggattatcc gataaaggag ctgacattct
    68881 atgcgaaggg tttcattatt gttactgatt cttaccgtgg cgttgctttt tagcgcctgc
    68941 ggtaaagatg aaccggaact gtcctgcatc tatccgggcc tttacgaaat caatcatgat
    69001 attaaatatt caggccagct ggtgatcctg aaacagcgtg ttcgctatca tgccgacggc
    69061 acgtttgaag ccacgaattt tcagaacaac gctgctctgg aggagatgaa gggcaaatat
    69121 aaagtagaaa acaagcagct gatttcttac gatacacaac gccgcctgat cactcaggaa
    69181 gggacgtgga caccgccgaa aaaggaaccc tcggccgtgg aacttcgcaa gatcaaaaag
    69241 ggaagctacc agtattattt caaatatcct gatgagcaaa tgcgtgaaaa atacaaggga
    69301 ctcggcctgg cagaaggctg gaagacgtac aagcgcatca gcgattagtt gccggacaac
    69361 cggacagcca tccggctgag gacaatgaaa aaaccgtcaa acaatatttc ggcgaattgc
    69421 ggtgccggcg aaaacctctg agtttagtct gtttccttat tgattagata tttggcaata
    69481 accgcattcg ttgaataatc ctgttttttg ccttttgcaa tctgccgcaa gactttcatg
    69541 tcggatttcc agaccaaatc gccgggtccg atgaaacagt agcttcccgg ggtgtattcg
    69601 gaactgatcg cgccgttttt gtaataatag tattctttgc ttgtctctgc aggataactg
    69661 cgtaccgtgg gcggaatatc cgtgggaaga aaccagagtt taatttcacg ttcagcatcc
    69721 tgaatatttg cggatgcatg aatcagattg tccgatcgat ctccgataaa ttttcccttt
    69781 gcgtcataca gcggaatgat cgttcccagt gagcggatgc agccgggtct ttgcgctctg
    69841 gcttcatgcg gattggtgtt accgcaaatc tccctgatgt ttttgatggc gttcggtccc
    69901 cggtagataa tcgcgatgac tctgcgcttc gagggttcat ccggataatg caggtacccg
    69961 cgaatgtatt caagcaaaga cggatagaaa aattttccct tgtgttcggc gtaatgttct
    70021 tctgccagcg ccagattgac ggcgacaacc ttcagcgccg cgaaacgcag tccggtgtgg
    70081 aactctgaaa atcgtgaaag gatatacccg gttagggaat tctttattgc atccggtttt
    70141 atcagaacca atgaattttc tcgattttgt tcaggcattt gaaactctcc ttatggttaa
    70201 agatgttaac aatcttcttt gtgagaagac atgcttaaaa agcgggcgaa gtataagaga
    70261 agtcctgctt tgtgtcaaca gaatatatcg ggccgaagtt cattgagctt ttggctggaa
    70321 atccacgcat tcagggactg tagagcctga tggacgatga gggcgtttct ctcttttttc
    70381 cgtagttttt tttccacggg tgcgttgaac agtccgaaat taatgttcat cggttgaaac
    70441 tgaactgcat gttcgggaga tgtgatatat tgaatgagag cgcccagcgc tgtgttgacg
    70501 ggaggcggtt cggctttttt cccttcgatg ataaacgccg cgttcagacc ggccagaagc
    70561 cccatggctg ccgactccat atagccttcc acgcccgtga tctgtccggc aaagaaaata
    70621 ttttcatggt ttttgagctg cagggtaggg ctcagcaggg cgggggagtt gatataggtg
    70681 ttgcggtgaa tgctgccgta gcgggcaaat tcaaccttct ccagtccggg aatcaggcga
    70741 aaaatccgcc gctgttccgg ccaggtcaat ttggtctgga aaccgaccag attaaaaaga
    70801 gtgccggcgg cgttctctct gcgcaattgc acaacggcgt agggtttgtt gccggtatga
    70861 ggatcaatca ggcccaccgg tttcatcgga ccgaatgcca ggctcttttc gccgcgctga
    70921 gccaggattt ctatcggaag gcagctctcg aaatggcggg tttcctcaaa atctctggcg
    70981 gccacctttt ctcccgctag caacgccttg tgaaatgatt cgtattcttc gcgggtcaga
    71041 gggcaattca ggtagtcggg cgttcccttg tcatagcgcg acgcccagaa caccttgttc
    71101 atatcaatgg aatccgcttc gacaatgggc gcaatggcat cgtaaaaata gagcgacgca
    71161 ccgccgagcc tccgggaaat atcatgcgcc attgcgtcgg aggtcagcgg gcctgtggca
    71221 atgatcgtta cagtatcgtt cgggatggcg gttacttcat gccggtaaag ggtgaaggcc
    71281 gcttgttcag aaagccgggc ttccacctcg cgggcgaaca gaaggcggtc aaccgccaga
    71341 gcggatccgg cgggcacggc ggttttatcc gccgctttca taatgaccga gtccagcagg
    71401 cgcatttctt ccttgagcaa accgggcgca ttatcgagag agttggactt gagggaattg
    71461 ctgcaaacca gttcggcgag attctccatt ttgtgggctt cggaaaattt ctgcggtttc
    71521 atttcataca tctcaaccgc atggccgcgc cgcagcagct gccaggccgc ctcgcagccg
    71581 gccagtccgc cgccgataat tgtcacacga tgatccatca ggattctgtt tttgttttag
    71641 ggattttttt gatgttcttg cattcgggat aaccggtgca gccgaggaac tggccgtagc
    71701 ggccgcgctt gacggccatg ggtttgccgc acagctcgca gacttcatcc gacagaacga
    71761 cctcctgttt ggccgccttt ccgtccttgt ccagcttttt gatgtttttg cattcgggat
    71821 aaccggtgca gccgaggaac tggccatagc ggccgcgctt gaccgtcatg ggtttgccgc
    71881 acagctcgca gacttcatcg gtcgtcggcg ttgcctgtgc gacgatttcg ccgttttcgt
    71941 ctttggccgc ggtcatcgtg tttttgcatt cgggataacc ggagcaggcg aggaactgac
    72001 cgaaacgccc ctctttgaca atcatatttt tgccgcactt gttgcatttg atatctgtcg
    72061 ttctggtttc cacgtgtttg acctttccgt tttcgtcatg agtaaaattc atggtgtttt
    72121 tgcatttggg ataattggaa cagcacagaa acttcccgtt tttcccccag cgaatcacca
    72181 tgggggactg gcatttctcg cagaccagat cggttggcgt ttcttcctgc ttgatgtttt
    72241 tcatttccgc cttggctttt ttcagctcgt cggcaaaaag cgcgtaaaat tccttgagcg
    72301 tctgcacacg cttgcgttcg cccgtttcaa tggcgtccag tttgttttcc atatccgcgg
    72361 tgaaagcgat ttccagaatg gtcggaaaac tttttaccag aagatgcgtc accatcattc
    72421 ccagttccga cggataaaac ttgccttttt ccagcttcac gtattcgcgt tcctgtattg
    72481 tggaaagaat cgcggcatag gtgctggggc gtccaatgcc tttttcctcc agttcgcgca
    72541 ccagcgacgc ttcggaaaaa cgcggcggcg gttgtgtgaa tttctgttcc gtattcagat
    72601 ggagaagttt caacttttct ttttcgctga cttccggcaa aaacttgtcc gttccgttgc
    72661 catttccgtt gccgttttca tccttgtcat ccttgccttc cgtatagacg atggtgaagc
    72721 cggggaattt catgatctgt ccctgcgcgc ggaaaagaca attggcgccg gcaatgtcaa
    72781 tggtggtctg atcgagaatg gccggattca tctgactggc gacaaagcgg ttccagatca
    72841 gttgataaag tttgaattga tcggcgctca gatattgctt gatgtcctgc ggtttattag
    72901 tcatgcggga cggacgaatc gcttcatggg catcctgcgc tttttgcgta tttttgtacg
    72961 tatgaggctt atgcggcaga taatccgcgg aataactttc tctgatgtag tcgcgaacat
    73021 ctttgatggc ttcttcggat atccggtagg agtccgttct catatatgtg atgagaccca
    73081 cggaaccttc cttgcccagt tcgattcctt catagagttt ttgcgcgacc atcatggtct
    73141 tcttggctga aaaacgaaac cagcgcgatg cttcctgctg caatttactg gtggtaaacg
    73201 ggggcggcgc agtgcgcttg acttctttct tttccagttt ttcaacaaaa aactccgcct
    73261 gcttgatttt atcggcaagc atcgcggcct gttcgccgtt ggtcactttt gcttttttgc
    73321 cgtcgacttt atgaagtttc gcttcaaaag gaggcggatt tttaccttcg aatttggccg
    73381 tcagattcca gtattcttcg ggcttgaaat tttttatttc gtcctcccgg tcgcagatca
    73441 tccggacggc gacggactga acgcgccccg cgctcaagcc tcgtttgacc ttgtcccaca
    73501 gcaccggact tatttgataa ccgaccagac ggtccagaat gcggcgggtc tgctgcgctt
    73561 catatttgtt gaaatccagt tgcaggggat ttttgatggc ctcggtcacg gtatctttgg
    73621 ttaaatcgtt gaagaggacg cggtagacgt ttttgttctt ggtgttgaga acttcattaa
    73681 tatgccaggc aatggcttcg ccttcgcggt ccgggtcggg ggccagataa atgttctcgg
    73741 cgttcttcgc cgcttttttc agttcatcga tggttttctt tttcgtttcg atgatgctgt
    73801 acgacggctc gaaatctttt tccagatcaa tacccagttt gcttttcggc aaatccttaa
    73861 tatgacccat cgaagcgacg gcattaaaat cgtttccgag aaattttttt atcgttttaa
    73921 cttttgtggg agactccaca atcaccaaag aatttgccat aatcccattc tctcttaaaa
    73981 aatagaaagc ccctataaat ggaagaaaaa aatttgtaaa gctatttttt acgatcttcc
    74041 tttcatttca ttatttaaat atccggaaaa taatagtttt attgattagt ttgttctttc
    74101 tgcagaaaaa aattttcccg ggtgctgccg gacaattcct ttgagttcca tggtggtcaa
    74161 agcgctgaga atatcggcgg acgaaaatcc cgtcacagaa atcaaatcgt cgatgtgcat
    74221 gcgtttttgg gaaaccaaat cgtataattt ctgctcggcg gcatttaatt tttccatggg
    74281 agggcttgca gcgtttaatg tcccgggagc ggaaaatcct tttagcggtt ttctctgttc
    74341 tatctgcggc agaatttctt ccagaatatc ctctgtgttt tcgatgagct tggctccctg
    74401 tttgatcagc ttattcgtac cgcgcgaacc ggcggaatca atgcttcccg gtacggcgaa
    74461 aacttcacgt ccctgttcca gcgccagcct cgccgtaatc aacgagccgc ttttttcgcc
    74521 tgcttccaca atgacaacgc cgtaggatat gccgctgata atgcggttgc gcgccgggaa
    74581 atttgtcgaa cgcggcggcg ttcccagggg gaattcggaa accacggcgc cgttttcgac
    74641 aatgtccgca aataattttt tattttccgg aggataaatg acatccaggc cgctgcccag
    74701 aatcgcaacg gttctgccat gagcggttat agcgccgcga tgcgccgacg aatcgatgcc
    74761 ccgagccatc ccgctgacca ccgtcacgcc ctgaagggcc aggtcccggc tgattctttc
    74821 cgtcgtgtat ttaccgtaag tgctcgctgc acgggaaccg acaatggcaa gattgatgtc
    74881 ttctctgctc aaatttcccc gcacatatat ataaggaggg cggtcataga tattcaataa
    74941 ttgccccgga tagagctcat cttcgtaagt aactattgta acgtcatttt tgcgaataag
    75001 ttcaagttct gctttaacat tatcccagcc tctgaaatga acaatggcct ctgcaatctg
    75061 acgggaaatt ccctctgttt gctgcaggtc ggaaacggaa gcaccgaaaa cagcggcagg
    75121 agagccgaac ctgtccacca gtgccggaaa agagacgtgg ccgatgcctt tgattgattt
    75181 taacgcaatc cagtacttta gaatctcatc attcataaat gacccgaacg agaagcgtta
    75241 tggtgatgat tggggatttt gaaaaattga cagcaaccta catcttgtct gccggatgtg
    75301 tcaagtattt tcaaaatgat gatccgccgg atgaacaaag agtgaaaaat gtgaaattct
    75361 taaaacagtt gcttaatttt aagctttgag ctaggaaacc atcaacgttt catagaggtc
    75421 gtcatgttca aggtaaaaaa tattctaatt ttttttatca caattttctt tatttacctg
    75481 cccgccggtt ttgctcaaaa cgtcacaaaa gtgcagaaac ctagcgttgc gcccaaacag
    75541 ggtgttcaaa agtcagtgga ccaggcaacg aaaaaaccaa aaccgggcct gaccattatg
    75601 ccggcgggaa ttaatttggg tcttgtcgcc tttgacaaat cgggcgaagc aaccgtcagt
    75661 ttaaaaaata cgggtaccgg cgcgatccgc tggtccatcc aggggcctaa gagctgggac
    75721 aaagtaaaat ccaaagcgat atcgggaaca ctggagaagg aaaccgatgt tttaaatgtg
    75781 ggtgtaagtt tactgggcaa tgatgaattc aaagacgaaa aaaaagccgg gcattcgctg
    75841 gcgaatgtcg aaatgagaat tgcctccgga gaggaagaaa ttatctgtac caagaaactt
    75901 ccgctaggaa tacataaaga ggaaattgaa atcaacgctg cggacgggca gaaaaaaata
    75961 accttgtctt ttttcattgc ctacacgcag agcgctcccc ggatcaacct cagtacgctg
    76021 agattggata tgggaaaaat cctgccgaat aaaacagttt ccagaaaaat aatattaaac
    76081 aacagcggta aggaaacgtt gacgtggtcg gtcgaaccgc cggcacacgc ccgggaagac
    76141 atgccggatg atttcaggca gggaaggtac ctgtccttca tgaatgatga ggtcaaaggc
    76201 gccgcacaat acagcgttcc cgagcctttg aaagacacac tcgagttaat cggaagctgg
    76261 tcggaaaccg gcggctatcc aaccagcggg ccgggcgata atctgatcaa agtcaatttt
    76321 accggaacgg ggatcctttt atacctgtcc gaacattccc gggaaggaaa actgaccctg
    76381 gcgctggata aaaatccaat cgcgagggcc gccctgtttg aagagcagag aaagaacggg
    76441 gaagaaatcc tgatagccga tcagctggaa tttggttccc ataccctcac gatcacgaac
    76501 aaatatgaag gtctggtcgt ggaaggcgta aaaatactgg gactgaacac agcctttttc
    76561 cccgctcaga gcataaaaat ctttcctgcg tccggggcca ccaccctgca gaccaattat
    76621 ctgaccgttt tatttaatac cgggcaggct ttcccgggct tttatgaaga ctacctggtc
    76681 tttaaaacta ataatggcga ggcgattgtc gagatgtatg cggaagtgct gccggatgtc
    76741 acctcggcga tcatcgatgt ttacagatat tacaacggaa acgactatct tttcacatcc
    76801 aatccgcagg cggaaatcaa cagattgtat cagaacaagt acacaaaaga agggatcgct
    76861 ttccgtttat tccagcccgg cacgccggga accgcgagct tctaccgctg gtatcatccc
    76921 cggaaaaaat gtcattttta tcattatgac tacaacggcc ataaacaaga tttgcacggt
    76981 tatattcttg aagggacgct cggcaacatc gccacctcga aactgaccaa cacccgggag
    77041 ctttaccggt ggtataatgc aaagacgggc cattattttt attcaacgga gttgcaggga
    77101 gaaaaaatta acaaaaaagc atatcgtttt gacggcatag ccggttacgt ccggtaacca
    77161 tcggaatgtt tcggaaaaat aatgcctcaa gggccgtatt tcatcttgac aaaaaaaaga
    77221 agagacgata ttaggctaag tctttttagg cacaggtaaa acatgcgcct gtagctcagt
    77281 tggatagagc aacggacttc taatccgtgg gccgagagtt cgaatccctc cagg
//
LOCUS       DAONQZ010000005        68443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_428_length_68443_cov_15.832427, whole genome shotgun sequence.
ACCESSION   DAONQZ010000005 DAONQZ010000000
VERSION     DAONQZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 68443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68443
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_68443_cov_15.832427"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..300
                     /gene="secE"
                     /locus_tag="PLR13_01875"
     CDS             85..300
                     /gene="secE"
                     /locus_tag="PLR13_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQC18106.1"
                     /translation="MEDFKEKVKVTIQKITQFLKDAKMELKKVTWPTPKQALASTAVV
                     IILVFIVSLILGIIDFALAKAVKLILG"
     gene            305..835
                     /gene="nusG"
                     /locus_tag="PLR13_01880"
     CDS             305..835
                     /gene="nusG"
                     /locus_tag="PLR13_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQC18107.1"
                     /translation="MAFKWYVVHTYSGYENKVKISLEERIKTTGTQAYFSDILIPEED
                     VVELISGEKKSSKRKFFPGYILVRMDMNDEAWHVVKDTPKVTGFIGSKDKPSPIPDKD
                     VEHLKIRIDEGTLKPKPKFKFDVGDHVKIIDGPFTNFDGIIDELKPEKSKLRVIVSIF
                     GRPTPVELDFIQVTQG"
     gene            891..1316
                     /gene="rplK"
                     /locus_tag="PLR13_01885"
     CDS             891..1316
                     /gene="rplK"
                     /locus_tag="PLR13_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQC18108.1"
                     /translation="MAKKIIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQSQEGMIIPVVITVYADRSFTFITKTPPVSVLLKQAAKIAKGSSDPKRDKVGSVTA
                     SQVKDIAELKYKDLNAVNIEGAINIIAGTARSMGIEITG"
     gene            1336..2034
                     /gene="rplA"
                     /locus_tag="PLR13_01890"
     CDS             1336..2034
                     /gene="rplA"
                     /locus_tag="PLR13_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQC18109.1"
                     /translation="MLRRGKRILAAKAKVEPAKKYTLKEAAEIVVSTAGTKFDETVDA
                     AIRLGVNPAHADQMVRGSVVLPHGLGKTVRVLVFAKGEKEKEALEAGADLVGNDEVIE
                     KIKGGWMEFDRVIATPDMMGSVGKIGKILGPRGLMPNPKVGTVTFDVANAVKELKAGK
                     VEFRVEKAGIVHSPVGKVSFGAEKLRENVSALLETILKLKPASSKGTYIKSISISSTM
                     GVGVRIDPLDIKAA"
     gene            2356..2880
                     /gene="rplJ"
                     /locus_tag="PLR13_01895"
     CDS             2356..2880
                     /gene="rplJ"
                     /locus_tag="PLR13_01895"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQC18110.1"
                     /translation="MDRKTKEQIVSELQKKLKEANLGILTSFSGMNVEKMEALRNELR
                     KSNAEWKVVKNTLLRIASEGTDFSILADHFKWPVAIVLSYKDPVAPAKVLIDFAKKNA
                     ELEIKVGVLDKKLLTRNDLTVLAELPGKDVLLGKLVSVMAAVPTSFVRVLNGVPGSFV
                     QVLNAYCDKKKSLN"
     gene            2906..3295
                     /gene="rplL"
                     /locus_tag="PLR13_01900"
     CDS             2906..3295
                     /gene="rplL"
                     /locus_tag="PLR13_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQC18111.1"
                     /translation="MSDKITKEDVVKFIEEMTVLELSALIKELEDKFGVSAAAPVMAA
                     VAAPGAAAAAGPVEEKTEFDVILTGFGAEKIQVIKVVRAITNLGLKEAKDLVEGVPKP
                     IKEGASKDEAADIKKKIEEVGGTVEIK"
     gene            3335..7465
                     /gene="rpoB"
                     /locus_tag="PLR13_01905"
     CDS             3335..7465
                     /gene="rpoB"
                     /locus_tag="PLR13_01905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQC18112.1"
                     /translation="MGDFRKNFGQVRKILDIPNLIDIQTESYEKFLQKDIAPVKRGAF
                     GLQGAFKSVFPISDFSGKCSLEFVSYKLGDIRYDVKECVQKGMTYAAPLKIVVRLVVF
                     EADRPAEGKGAGETTEAKQIRDIKEQEVYFGEIPLMTENGTFIINGTERVIVNQLHRS
                     PGIFFDHDKGKIVASGKLIYSARVIPIRGSWLDLEFDSKDILYVRIDRRRKMPVTILL
                     KALGNSTEFILNYFYNVNTVINDNKKIYFAVDESLVGKKSPEDIKDSKSSELIVKKGR
                     KLTKPLIKRIMEAGVKRTAINENELIGKIPSRDVHDPETGEIVFRCNEELPANCLDII
                     REKNIRELKLIHIDEDMDNASIRDTLLLDKIDTTEDAIMEIYRRLRPSNPPTPETADK
                     FFKSLFFEPETYDLSNVGRAKMNYKLHMNVPTDNTVLSNEDILSAVKYLIDLKNGVGD
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFNSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHY
                     GRICPIETPEGPNIGLIVSLSTYARVNEFGFIETPYRKVENGRVSDDVHFVTAIEEEN
                     QIIASADRPLSKNGKFAEELISVRKGGSYISVEPEEIKMMDVSPTQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMNPEVPVVGTGVESVVARDSGAVVLAKRSGVVESVDA
                     SRIIVRADRMDEDGIDSGVDIYNLAKYQRSNQDTCFNQKPIISVGQRVHKKDILADGP
                     AIDNGELALGRNVMVAFMSWGGYNYEDSILVSERVVKEDIFTSIHIEEFETMSRDTKL
                     GKEEITRDIPNVGEEVLKNLDESGIVCIGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGAESDSRSQAIEADAIEQIAKDRD
                     DEIRILTETIKKDITKLVVGKTAATRIGDAKKKTVLKKGDKITKEIWNDLPFSSWKDI
                     TIAENDGSEEKIAGLIASLERKIDFVSAHFEEKMEKIKASDELPPGVIKMVKVYIAIK
                     RRLSVGDKMAGRHGNKGVLSRILPEEDMPYFADGSTVDIVLNPLGVPSRMNVGQILET
                     HLGWAAKSIGEGLNELLEQQRAGLSHIKDELKKVYSSPDFDKFIDQASTEDIKRFVGR
                     LKKGMLVASPVFDGCPEDQIREMLKRTDLPETGQSILFDGRTGEPFDQEVTVGIIYMM
                     KLHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYALQEFL
                     TVKSDDVAGRTRIYESIVKGEHNFESGLPESFNVLVKELQGLGLDVELVEESDSLS"
     gene            7546..11640
                     /gene="rpoC"
                     /locus_tag="PLR13_01910"
     CDS             7546..11640
                     /gene="rpoC"
                     /locus_tag="PLR13_01910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQC18113.1"
                     /translation="MEDVFSYFEKPKNLVKFNAMQISIASPDKILSWSHGEVKKPETI
                     NYRTFKPEKDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNAVDLSLKELEKVLYFESWIVLDPKNTPLKKKDLL
                     SDEEYEEAREQYGNDGFVAGIGAEAIRQLLEEIDLEKLYEELRTEIVTSVSDTKKKKL
                     VKRLKVVEALRKSGNKPEWMILTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVVTVGPDLRLHQCGLPKKMAIELFKPFIYNRLLEKGYV
                     TTVKSAKKMVEKESAEVWDALDEVVREYPIILNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHIPLSVEAQTEARVLMMSTNNILSPANGSPIIIPSQDIV
                     LGIYYLTRAKTGVKGDGMIFAGPDEVRAAIDNGVIDLHARIKVRIDSELKETTAGRII
                     LSEIVPEQISFDDINKLMNKKELTSLIDHCYRLCGDKTTIILADKLKDLGFKYATVSG
                     LSFAVGNMIIPEQKKKIVLQADKDVLKIQRQYMDGLITDGERYNKVVDIWAQATEKIA
                     SEMLKGISTEDVPAAEGKKRKIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSG
                     EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIIT
                     EHDCETVDGVYVSALMEGGEIIETAGERVLGRVALQDVKDPFSGEVIVPANQMIDETL
                     AEKIDNAGIEKVHIRSVLTCRSKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEP
                     GTQLTMRTFHIGGTAKFDEHSTLEARHDGIVKFVNLSTVKTKEGDLVVMKRNGEVHVL
                     DEQKHVRGKYVLSYGAHLKVTDNSEVKKGQLIAEWDPFSIPILAEVDGTIKFDDITEG
                     KTMQEQVDEVTGLSRKIIVEFKGGDLRPRVSIKDEKGKTAKLADSNAVARYLLSVGVN
                     LVVSEGDQVRAGDIIAKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEISGVVS
                     YGKDAKGKRKIVVTPEVGEEKEYLIPKGKHISVQEGDVVSPGDALMSGVNNPHDLLSI
                     KGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVKVMDPGDTAFIADEKIE
                     RYRFQEENEKIIAQGGRPAIGEPMLLGITKASLSTQSFISAASFQETTKVLTEAALSG
                     KTDYLRGLKENVIMGRLITAGTGLVMYRKLGIALENENAETAAE"
     gene            11752..12123
                     /gene="rpsL"
                     /locus_tag="PLR13_01915"
     CDS             11752..12123
                     /gene="rpsL"
                     /locus_tag="PLR13_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQC18114.1"
                     /translation="MPTISQLVRKGRAKIKRKTNSPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHVIRGTL
                     DTVGVQDRKQGRSKYGAKKPS"
     gene            12138..12608
                     /gene="rpsG"
                     /locus_tag="PLR13_01920"
     CDS             12138..12608
                     /gene="rpsG"
                     /locus_tag="PLR13_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQC18115.1"
                     /translation="MPRRREVEKRDILPDPKFNNKLVAKFINSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVDFFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLASELIDAANNRGGAIKKKEAVHKMAEANKAFAHYKF"
     gene            12665..13858
                     /gene="tuf"
                     /locus_tag="PLR13_01925"
     CDS             12665..13858
                     /gene="tuf"
                     /locus_tag="PLR13_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQC18116.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTVTAAITKYLAKKGFAEF
                     RAFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYIVVFLNKVDLVDDPELLDLVELELRELLTQ
                     YEFPGDNIPIIRGSALKALEADDINSPDVKCIAELMDAVDNYVPEPKRDTDKPFLMPV
                     GDVFTISGRGTVVTGRVDRGIVKVGEEVEIIGIRETIKTVVTGVEMFRKTLDEGRAGD
                     DVGLLLRGIKREEVERGQVVAKPASITPHTKFEAAVYVLTKEEGGRHTPFFSGYRPQF
                     YFRTTDVTGVATLPEGVEMVMPGDNVKMSIELITPIAMEEQLRFAIREGGRTVGAGVV
                     SKVIA"
     gene            13878..14186
                     /gene="rpsJ"
                     /locus_tag="PLR13_01930"
     CDS             13878..14186
                     /gene="rpsJ"
                     /locus_tag="PLR13_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQC18117.1"
                     /translation="MKEQKIRIRLKAYDYKLLDRSVEEIVETAKKTGARVAGPIPIPT
                     EINKYCVNRSPHVDKKSREQFEIRTHKRLVDIVGPTQATVDALMKLDLSAGVDVEIKA
                     "
     gene            14275..14901
                     /gene="rplC"
                     /locus_tag="PLR13_01935"
     CDS             14275..14901
                     /gene="rplC"
                     /locus_tag="PLR13_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQC18118.1"
                     /translation="MSKGIIGKKLGMTQVFADDGSAVGVTAIEVEPSVVLQVKTKDKD
                     GYDAVQLGYGRVKQKNVTRPLQGHFNKANKGFFRFVREFPAHSETYEVGQEISADIFA
                     MGDYVDVVGTSKGKGFQGVVKRHGFGGGRATHGSMFHRAPGSIGASADPSRVFKGTKM
                     GGHMGNVRKTVQNLKVWQVRSERNLLLLEGSVPGSRNSFLLITKAKKK"
     gene            14930..15553
                     /gene="rplD"
                     /locus_tag="PLR13_01940"
     CDS             14930..15553
                     /gene="rplD"
                     /locus_tag="PLR13_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQC18119.1"
                     /translation="MAVADVLNIEKKKVAEVDLNDAVFGADVNEAVIYDVVKMQLASR
                     RSGTASTKTRSDVSGGGKKPFRQKGTGRARAGSSRSPVWRTGGTVFGPHPRDYSFSVP
                     KKIRKKALISALSLKFKENKMIILKDFPMEKISARGFQKVVDLFDLKKALFVLDDNNE
                     ILMKSSRNIKNVKMIRSEGINVYDVLNHEHLILLEPSVKKIEGALLV"
     gene            15553..15840
                     /gene="rplW"
                     /locus_tag="PLR13_01945"
     CDS             15553..15840
                     /gene="rplW"
                     /locus_tag="PLR13_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQC18120.1"
                     /translation="MDRSKVVKRILVTEKSTMARDASNKYFFEVDRKANKVEIADAVE
                     KLFKVKVMDVRVMHVLGKKKRMGRTVGQKSSWKKAVVTLAAGNSIEFAEGV"
     gene            15868..16692
                     /gene="rplB"
                     /locus_tag="PLR13_01950"
     CDS             15868..16692
                     /gene="rplB"
                     /locus_tag="PLR13_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQC18121.1"
                     /translation="MAIIKYKPTSPGRRFQSVSDFAEITSTEPVKRLLKPLKKKGGRN
                     SYGRVTARHIGGGHKRKYRVIDFCRNKADIPAKVASIEYDPNRTARIALLNYRDGEKR
                     YIVAPAQVNVGDVLYSGEKADIKPGNAVPLKYIPLGSLIHNVELKVGRGGQLIRSAGA
                     YGQLMAKEGSYAQVRLPSGEVRKIFIECMATIGQVGNNEHENISIGKAGRTRWLGKKP
                     HVRGVVMNPVDHPMGGGEGKSSGGRHPCTPWGIPTKGHKTRKNKRTDKYIIKRRGK"
     gene            16695..16973
                     /gene="rpsS"
                     /locus_tag="PLR13_01955"
     CDS             16695..16973
                     /gene="rpsS"
                     /locus_tag="PLR13_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQC18122.1"
                     /translation="MARSIKKGPYIEEKLLAKIRKMEAEGTKNVIKTWSRRSTITPEL
                     IGYTFAVHNGKKFIPVYVTENMVGHKLGEFAPTRVFFSHSGDRKTKVS"
     gene            17016..17348
                     /gene="rplV"
                     /locus_tag="PLR13_01960"
     CDS             17016..17348
                     /gene="rplV"
                     /locus_tag="PLR13_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQC18123.1"
                     /translation="MEAKAVAKYMRMSPQKVRLVADLIRGKNVQEARNILLYTRKYAA
                     GVVANVLKSAVANAAQNPNIDENILYVKEIYVDQGPALKRWRARAQGRAAGIKKRSSH
                     ITVILDEI"
     gene            17360..18001
                     /gene="rpsC"
                     /locus_tag="PLR13_01965"
     CDS             17360..18001
                     /gene="rpsC"
                     /locus_tag="PLR13_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQC18124.1"
                     /translation="MGQKVNPVGLRLGYIKKWQSVWYTDKKYSDVLHEDLQIRKYLKK
                     KLYHAGLSKIEIERAANKAKVNIYSARPGVIIGKKGSEIEKLKSEIERFSQSEIIINI
                     LEVRKPEVDAQLVAENVAMQLEKRVAFRRAMKKCVGYALKFGAKGIKIACSGRLGGAE
                     MSRSEWYREGRVPLHTLRADIDYGFTEAKTAYGLIGVKVFIFHGEVLPTKNEK"
     gene            18080..18502
                     /gene="rplP"
                     /locus_tag="PLR13_01970"
     CDS             18080..18502
                     /gene="rplP"
                     /locus_tag="PLR13_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQC18125.1"
                     /translation="MLMPKRVKYRKLQKGRMGGKATRGSSIAFGEYGLQAAECGWITS
                     RQIEAARVAMTRHVKRGGRIWIRVFPHKSVTKKPAETRMGKGKGAPEGWVAVVKPGSV
                     LYEMEGVSKDVAREAFRLAAHKLSIATKFLSRDIVDEN"
     gene            18492..18686
                     /gene="rpmC"
                     /locus_tag="PLR13_01975"
     CDS             18492..18686
                     /gene="rpmC"
                     /locus_tag="PLR13_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQC18126.1"
                     /translation="MKIKEIRALDSNGLKQKIAELSEELFRLKIRHASGQLESTVMLS
                     KIRKDIARVNTVLKEKEAAS"
     gene            18688..18945
                     /gene="rpsQ"
                     /locus_tag="PLR13_01980"
     CDS             18688..18945
                     /gene="rpsQ"
                     /locus_tag="PLR13_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQC18127.1"
                     /translation="MDQRGFNRTIKGVVVSNKMDKTIVVRAERFVKHPVFHKYVRKHV
                     KYKAHDERNQSKIGDTVLIIESRPLSREKRWRMLEILEKAK"
     gene            18991..19359
                     /gene="rplN"
                     /locus_tag="PLR13_01985"
     CDS             18991..19359
                     /gene="rplN"
                     /locus_tag="PLR13_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQC18128.1"
                     /translation="MIQMQTILNVADNSGAKKVACIKVLGGSKRRYASLGDVIVVSVK
                     EAIPNSKVKKGDVIKAVIVRTVKEVRRPDGSYLKFDDNSAVLISNQMEPVGTRIFGPV
                     ARELRARQFMKIISLAPEVL"
     gene            19382..19696
                     /gene="rplX"
                     /locus_tag="PLR13_01990"
     CDS             19382..19696
                     /gene="rplX"
                     /locus_tag="PLR13_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQC18129.1"
                     /translation="MSLNKFKKGDTVKVLAGKDKGKTGKVLAVIPEKDRIVVEKVNVI
                     KKHKRPDQRSKGGIVEKEGSIHVSNVGLFCNKCNKPVRVQSKKLEDGKKVRICSKCKD
                     VI"
     gene            19712..20251
                     /gene="rplE"
                     /locus_tag="PLR13_01995"
     CDS             19712..20251
                     /gene="rplE"
                     /locus_tag="PLR13_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQC18130.1"
                     /translation="MARLKEYYSKTVAPALVKEFKYKNPMQVPKMEKIVINMGLGEAI
                     QNVKIIDGAVQELAAITGQKPIVNKAKKSIATFKLRQGMPVGCSVTLRKKIMYEFFDR
                     LVNAALPKVRDFRGISPHAFDGRGNFSIGLQEQIIFPEIDYDKIEKNKGMNITIVTTA
                     KTDEEARFLLKMMGVPFKQ"
     gene            20278..20463
                     /locus_tag="PLR13_02000"
     CDS             20278..20463
                     /locus_tag="PLR13_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQC18131.1"
                     /translation="MAKKSLIAKAKREKKFSVREYNRCPLCGRPRAYYRKFDMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            20488..20889
                     /gene="rpsH"
                     /locus_tag="PLR13_02005"
     CDS             20488..20889
                     /gene="rpsH"
                     /locus_tag="PLR13_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQC18132.1"
                     /translation="MGMTDPIADMLTRIRNANKARFKTVRVSMSQVNVNVAGVLKKTG
                     YITNYEKVKDEKGRQMLEIILKYPDAKRTVITNIKRVSKPGRRIYVPADNIPKVLNGL
                     GISILSTSRGVITDAEARELNVGGEILCNVW"
     gene            20925..21464
                     /gene="rplF"
                     /locus_tag="PLR13_02010"
     CDS             20925..21464
                     /gene="rplF"
                     /locus_tag="PLR13_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQC18133.1"
                     /translation="MSRIGKKPVSIPKNVKISQSADTVKVEGPKGALSSRMPEGIAIK
                     ISDGTLSVERKSEALTTRSYHGLTRTLIDNMIKGVTTGFEKRLEISGVGYRAEANDKS
                     LKFILGFSSPVQYDIPKGIDIKIDKQVNIVVSGIDKELVGRVAAEIRALRKPEPYKGK
                     GIKYANEYIKRKAGKSAGA"
     gene            21503..21868
                     /gene="rplR"
                     /locus_tag="PLR13_02015"
     CDS             21503..21868
                     /gene="rplR"
                     /locus_tag="PLR13_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQC18134.1"
                     /translation="MASKKTLKIREKREKRTRSKIKGTQERPRLSVFRSDRHIYVQAV
                     NDVTADTLATASTLCKELESRLKGKKKVDVAKEVGKLIAERLKAKGIEKVVFDRGRFL
                     YHGRVKALAEAARESGLKF"
     gene            21926..22390
                     /gene="rpsE"
                     /locus_tag="PLR13_02020"
     CDS             21926..22390
                     /gene="rpsE"
                     /locus_tag="PLR13_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQC18135.1"
                     /translation="MKDMELLDRVVAINRTAKVVKGGRRFRFSAIVVVGDGKGMIGTG
                     LGKAHEVPEAIRKGMDMAKKNMVRVAVVDRTIPFEVIGRYGAGKVLLKPASEGTGLIA
                     GGAVRAVLEVAGVHNVLTKCLGSHNPHNLVKATFEGLCQLKNPEEIAAQRGK"
     gene            22431..22610
                     /gene="rpmD"
                     /locus_tag="PLR13_02025"
     CDS             22431..22610
                     /gene="rpmD"
                     /locus_tag="PLR13_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQC18136.1"
                     /translation="MLKITKVKSVIGRPEKQRKILKGMGLTKLNKTVTLEDTPQIRGM
                     INKVTHLVSVEESKE"
     gene            22614..23060
                     /gene="rplO"
                     /locus_tag="PLR13_02030"
     CDS             22614..23060
                     /gene="rplO"
                     /locus_tag="PLR13_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQC18137.1"
                     /translation="MDLGSLKAPAGATKKRKRVGRGNASGHGGTSGKGHKGQKARSGG
                     KVRVGFEGGQMPMARRLPKRGFRNPFRKEYIAVNILDLCRKFSKGAVIDEAALLQSGL
                     IKTEGAHIKILAKGDIDFPVTLKIARISPAAKDKIIAAGGSVEEVI"
     gene            23062..24369
                     /gene="secY"
                     /locus_tag="PLR13_02035"
     CDS             23062..24369
                     /gene="secY"
                     /locus_tag="PLR13_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQC18138.1"
                     /translation="MLEGLGSVSKIPELQRRILFTILLLGVYRIGVYIPTPGIDNVAL
                     LAYFEHAKGSLFGLMDMFAGGAFSNFSIFALGIMPYISSSIILQLLTIAVPHLERLSK
                     EGESGRRKITQYTRYGTVLLSMIQGFGIAFGLQQMAGPSGAPIVLNPGIGFVLMTMIT
                     LTSGTAFIMWLGEVITEKGIGNGISLIIFAGIVCRIPAAIGNTFRLATAGELGVLVIL
                     LITVLMIAVVGAIVFVEGGQRRIPVQYAKRVVGRKMYGGQTTHLPLKVNTSGVIPPIF
                     ASSVIMFPATIANFAPQTWMKDIAAYLVPGRLAYEVLYVAFIFFFCFFYTAVTFKPDD
                     VAENMKKYGGYVPGIRPGKKTAEYIENVLEKLTFSGAIYVSIICVLPSVLIAQLNVPF
                     YFGGTALLIVVGVAMDTASQIESHLLTRQYEGFMKKGHIARRR"
     gene            24382..25134
                     /gene="map"
                     /locus_tag="PLR13_02040"
     CDS             24382..25134
                     /gene="map"
                     /locus_tag="PLR13_02040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQC18139.1"
                     /translation="MVILKLPEEIERARASNRIVAEVLSRLREKVKPGVKTKDLDKFA
                     EEIAEKRGARPAFKGYHGYPHSLCVSINEVVVHGMPSERVLEEGDIVGLDFGVFYQGF
                     FGDATVTLPVGKVTAKALRLMQVTEQSLYAGIAQAREGNRLGDISAAVQSVVEEAGYS
                     VVRDFVGHGIGKDLHEEPQIPNFGQKGRGIELKKGMILAIEPMVNEGKYKVQVLPDGW
                     TVVTKDGSLSAHFEHSIAITEHGPDILSRLNY"
     gene            25156..25374
                     /gene="infA"
                     /locus_tag="PLR13_02045"
     CDS             25156..25374
                     /gene="infA"
                     /locus_tag="PLR13_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQC18140.1"
                     /translation="MAKEEAIEVEGRVIEPLPNAMFRVELDNGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            25393..25506
                     /gene="rpmJ"
                     /locus_tag="PLR13_02050"
     CDS             25393..25506
                     /gene="rpmJ"
                     /locus_tag="PLR13_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQC18141.1"
                     /translation="MKVRSSVKKICEKCKIVKRKGVLRVICENPKHKQRQG"
     gene            25532..25900
                     /gene="rpsM"
                     /locus_tag="PLR13_02055"
     CDS             25532..25900
                     /gene="rpsM"
                     /locus_tag="PLR13_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQC18142.1"
                     /translation="MARISGVDLPKNKRMEIALTYIYGIGKTRAKQILKDAGVSPDTR
                     TDDLADSEISAIRGIIDKEGKVEGDLRRDISMSIKRLMDVGTYRGLRHRKGLPVRGQR
                     THTNGRTRKGPRRAIAGKKK"
     gene            25927..26319
                     /gene="rpsK"
                     /locus_tag="PLR13_02060"
     CDS             25927..26319
                     /gene="rpsK"
                     /locus_tag="PLR13_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQC18143.1"
                     /translation="MAKAVRKTGKKKEKKNITDGIAHIQSTFNNTIVTITDMSGNVIA
                     WSSSGLQGFKGSRKSTPFAAQMAAEDVVRKAKEQGMRKVQVYIKGPGAGRESALRSLQ
                     IAGLTITLIRDVTPVPHNGCRPPKRRRV"
     gene            26367..26996
                     /gene="rpsD"
                     /locus_tag="PLR13_02065"
     CDS             26367..26996
                     /gene="rpsD"
                     /locus_tag="PLR13_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQC18144.1"
                     /translation="MARYTDSSCRLCRREGLKLFSKGDRCYSEKCSFERRDYVPGDHG
                     QMHKKQRSDYGVQLREKQKLKRMYGLLEKQFRSYFEKADKQKGITGTNLLILLERRMD
                     NMVFRMGFANSRIESRQLISHGHFLVNGKPVNIPSYLLKAGDEVSVKENSRKVTRIMG
                     ALETVVRRGVPQWLELDKDNFNAKIKTLPVREDLTMPIQEQLIVELYSK"
     gene            27030..28010
                     /locus_tag="PLR13_02070"
     CDS             27030..28010
                     /locus_tag="PLR13_02070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQC18145.1"
                     /translation="MGLIMQRNWSSLIRPKRIDVDEATHTNFYGEFTIQPLERGFGIT
                     LGNALRRVLLSSIQGSAIVSAKIEGVLHEFSTISGVKEDVTDIILNLKGVRFKLGQAN
                     IKEVSIDVSRAGEVTAADIMTDGTVEILNPEHHIATLSSGSFKAKMMVKMGKGYVPAK
                     KELDPDQPKGTINIDAVFSPIKKVNYVVSYARVGQIADYDKLTLEVWTDGSVKPEDAI
                     AYSAKILKQQLDVFINFEEVEEEILPEKQDEPDNINKILSRSVEDLELSVRSANCLKN
                     AGINTIGELVKKTEAEMLKTKNFGRKSLSEIKDILAEYDLTFGMDIGSKN"
     gene            28074..28496
                     /gene="rplQ"
                     /locus_tag="PLR13_02075"
     CDS             28074..28496
                     /gene="rplQ"
                     /locus_tag="PLR13_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQC18146.1"
                     /translation="MYHGKAGRKLGRTSSHKEAMLRNMVTSVIKHERIRTTDAKAKEL
                     RKLAEKMITLGKKGSLHARRQALAVIRDKETVGKLFGEMTERYRNRAGGYTRIVKAGY
                     RYGDNAPVSILEFINDEKKKEKAKPKARSRAKAKEKAE"
     gene            28633..28794
                     /locus_tag="PLR13_02080"
     CDS             28633..28794
                     /locus_tag="PLR13_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18147.1"
                     /translation="MGFNGGELTVMTLPLALGRPGSLSMIQQTQALKTGNEAVLDIID
                     CPVRVYAFR"
     gene            28816..29076
                     /locus_tag="PLR13_02085"
     CDS             28816..29076
                     /locus_tag="PLR13_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18148.1"
                     /translation="MAEKKIGEVMKFFSKPSVAAVKITDGEIAVGDSLKFSGHTTDFT
                     DVIESMEVDNQSVPKAVAGDFIGIKVSDRVRPGDEVFKVIPD"
     gene            29197..30021
                     /locus_tag="PLR13_02090"
     CDS             29197..30021
                     /locus_tag="PLR13_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HQC18149.1"
                     /translation="MKSRIAEAIKLKYQPVALLWSVEKPADAMQFAEGKWGCVMWLAV
                     HAAKGRAAVADRKTFGCFGGGVGLGFGNQYKNFPGGEEGFCYFLSSGNARRPGGPEMA
                     AKVKPFMTRESHDNFLNGERYVQNPQLVEKFIRDLPLTDIPKPFVVFQPLSDVDQDGE
                     KPQTVIFFVNPDQLSALTVLANYGREGNENVIIPYAAGCQAIGIYPYREAGLKNPRAV
                     VGLTDLSARVYVRKQLGESHLMTFSVPFNLYDEMEQNVPGSFLERHTWQSLLSENR"
     gene            30109..31419
                     /locus_tag="PLR13_02095"
     CDS             30109..31419
                     /locus_tag="PLR13_02095"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQC18150.1"
                     /translation="MKNSRTSQYLDSLNINKMRFGLGAITSVLSRLGHPQKSFKAVTI
                     TGTNGKGSTAAMIAAMLHKAGYCVGLYTSPHLIDVRERITVNGRKISRKDFDRIIVCV
                     RKKVVQPLTYFEFITAVAFEYFKTRKADIAVLEVGLGGRLDATNVCRPLVSVITNIAF
                     DHTAYLGRTLTSIAVEKAGIIKPHGLCVTGVAQKKILGLLQDICLQRGASLYRLNHDF
                     RLARQKNGSLLYEGVYSDVKNLTVPLRGEHQLANAALALAAVEMCARKGFPVSETAVR
                     AGLQNVRWEGRLEVLHISPWVLLDGAHNPAGIGALCRSLKKDFSFRHLILIFGVLADK
                     DYRKMLQKIVPLARVIILAPLKAQRAASVAAVSQTIRSLGRMPVVSQSVHQALELGLS
                     LAGSRDLICATGSFYLAGEVKQTFSKLVFCDNEKSKKVQGKKVS"
     gene            31416..33758
                     /gene="lptD"
                     /locus_tag="PLR13_02100"
     CDS             31416..33758
                     /gene="lptD"
                     /locus_tag="PLR13_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HQC18151.1"
                     /translation="MNINTLINVKPIKHNGLASFSGCLEPVFTFCLVAVIIILWSFPA
                     DCAGKQKSSAGKASPSIQKKNFPASVHTNEKSPVSIEADAMDYDHVSDLYRARGQVSV
                     FYSGASLYADEVELDNQNHVARAQGNTLLKIGEDTLKGERLWFNIEDKTGAAFNADAF
                     YARNHFRVRGNIIEKTGENTYFIDNPSATTCDGSNPDWEIAGSEMKVTIEGYGLMKNA
                     CFKVGSLPLLYSPYILFPAKTKRQTGFLLPYLAYSRDKDGIDVELPFFWAVSPWMDAT
                     FYQRFIEKRGFKEGAEFRYDFGNKSFGTFYGDYLEDTKQVTETEDDALRRDWQGMHKR
                     WSYFLHHQTRLDSQFYLRTDLKKVSDKWYFRDFSSYNYYREHFNSTSEDRFRNVSFEG
                     DKSLRYLDSTIRVYKGWSKYNVTGLMHAADDFAAVNNDQTLQRYPEIIFAGVKQPLLH
                     TPFYYELAGRYDYLYKKEGHKGHFADLSPSLSMPVNLSDYVRIIPQFTFKETFWSRDD
                     DENGLKEKTADRSVYNASVSFGSQLFRVFHLNGKHWEKIRHEIKPEIIYSYMPSISAE
                     DVPDYYLPALSPFGMPLASLSSDPLNGQNAVAWSLTNTLTARVKDQGGSLSYLEFFRL
                     KVFQAYDIDEARRDMARNAPERRPFSDMGIEFDFSPHKYFSFRMRDKYNVYSGWKQNS
                     YDLSLRDWRGDALLVAYRNTEGLVEEINLGVKAVITDHFYSTFVLKRDLQDARKIENS
                     LGFVYHTQCWSLGFDLSDTDDDVRFLFRISLAGFGKTADK"
     gene            33855..35372
                     /locus_tag="PLR13_02105"
     CDS             33855..35372
                     /locus_tag="PLR13_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18152.1"
                     /translation="MDTFILVATSFTIAVSLMITGKTNRLQSAFAALCAAVFVSQAAV
                     TLENIFHYEFLLKIKYLGSLAIAPAALRFFHHLTKGQSIILRNFFSLFAVISLCGVVF
                     VFLPLSEKTCFHNVIAAYVLFSLMICYLALLGHVGTLPSGTEKRRFGYLLVFCPVALI
                     LSYLDLLNFWGFSLLKINGMVSSALLYFILLIIAYPQLLELHDFLARSLVILLSTLAG
                     AAIFYFTVSFFNGDPPLTGLLMASFLVVISLSPMRMIFRKIFGVFYPDSKDIFTSLYE
                     FDEKLEREKALMLEEMAPVLAHEIRNPLGSIKGAAQYLKTEASTGEHKELLDVIVEGT
                     DRLNTVVSKFLDYARPYQLQLKTQDINAIIRRAISVIAANKLLEHITIVEDLEDKLPF
                     AAVDEQQCLQVVLNIALNAIEAMPQGGTLTFCTSSQKSETAEDLALTIRDTGTGIDQK
                     YVKDIFKPFFTTKERGVGLGLAICQKIIREHNGRISVQSIASQGTTVVIQLKRSG"
     gene            35426..36799
                     /locus_tag="PLR13_02110"
     CDS             35426..36799
                     /locus_tag="PLR13_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC18153.1"
                     /translation="MKKILIVDDELNMRLVLSAMLKKEGFEVSSASNGREALQILQSN
                     KMALVITDLKMPGMDGMDLLTQISANHPQIPVVMITAHGTIATAVEALKKGAFDYITK
                     PFDLDDLKNIVSKAIRTSTLKESEFSMAPSEIDRLGIIGTSPAIMEIFEAIHRVAQTT
                     TTVMITGETGTGKELVADAIHYNSQRKNNPLIKINCAAIAETLMESELFGYEKGAYTG
                     AAVTKPGKFELAHKGTLFLDEVAEIPREMQVKLLRVLQEQAFERVGGLRTIKVDVRII
                     AATNQHLLRQVQTGNFREDLYYRLNVFPIDIPPLRERKDDILSLADYFLDKFNKKLGI
                     SVGMDNEVKDILLRYDWPGNIRELENLIERMMLLARGCRIMSGDVPDDFKKSVDHVNT
                     ALSPGIKKPFKEYVRDQVENVERQMIARALDESGGNITQAAKKMGLSRKGLQLKMSKY
                     HLRKESK"
     gene            36840..37049
                     /locus_tag="PLR13_02115"
     CDS             36840..37049
                     /locus_tag="PLR13_02115"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQC18154.1"
                     /translation="MNFKIGLVAIFALLALIFLVQNIEVMTVHFLFWQLSMSRAIFLF
                     FILLTGFIIGWFLHSFVSYSQKKKN"
     gene            complement(37100..37942)
                     /locus_tag="PLR13_02120"
     CDS             complement(37100..37942)
                     /locus_tag="PLR13_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HQC18155.1"
                     /translation="MNAKSALIIAAIVFIFTGCASENIQVRKLPDSAINAGLEDLRQE
                     VADNGVPGEWFDDQMDHYAFRLHHNIDRFFRKSAEKKTDSEKKRSLLWYFSIFGVETK
                     IEKGKTFIEDYTGILERAEAKHGIHKELIAAIIGMETNFAQRRYRGKFYAFNSLVSQY
                     IFTDRKKFAVREITSLYKFSEKTDRSPWYFKSSYAGAIGWGQFIPSSLLSFFIDENGD
                     NDDIDPFSIEDTIFSVENYLYRHGLSGKNVHKRRSRYKAVYAYNHSDAYVQAVLHIYD
                     GLKN"
     gene            38147..38914
                     /locus_tag="PLR13_02125"
     CDS             38147..38914
                     /locus_tag="PLR13_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18156.1"
                     /translation="MFHSRKYPTKDHYPFHLPLFRATKRLVFDTPVTLFVGENGTGKS
                     TLIEALAQACQIHIWRDTQRTRPDYNPYEDQLHRYMSVIWANEPVPGSFLGSKNFQYF
                     AEKLDEWASADRDQLNYFGGRSLTTQSHGQSLMSFFYSRYRIPGLYLLDEPETALSPQ
                     SQILLLDILQRMSAAGHAQFIIATHSPLLLACPQATIYNFDRSPLTKIVYEKTKHYRI
                     YKDFLLNRSNYLKPKAKHQRKDTKCLPKKKSLTKQSA"
     gene            38872..40002
                     /locus_tag="PLR13_02130"
     CDS             38872..40002
                     /locus_tag="PLR13_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HQC18157.1"
                     /translation="MLTKEEIIDEAKRLKFADIGFTGAEPFGSQKDYLLTHQEEYGWA
                     EAIGLGLLAGTDPRNILAGAKSIIVVLESYFEESFPQPMERHFGRCYLDDDRITQDGL
                     ALKIKAFRKYLKANGVDSKVPFHLPHRLSAARAGLGTFGNNCIFYARRVARGSSFVFP
                     IALVVDCEFTPDEPSVAMGCPDWCRNACVAACPTRALKGGGKIDPRRCISFLTYYGSG
                     LTPQDLREPMGMYIYGCDRCQNVCPRNIPWMAEEKPVNQRVSAKASDFGLSRLLHMDA
                     VYFEKKIWPHMFYMSPDDLWRWKMNVARAMGNSLNPVYVADLIRAFHENSDERVRCMA
                     AWALGRIGGNEAVEALGRFLKNSDGVVREEIEQAMVSASAGV"
     gene            complement(40013..40492)
                     /gene="greA"
                     /locus_tag="PLR13_02135"
     CDS             complement(40013..40492)
                     /gene="greA"
                     /locus_tag="PLR13_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQC18158.1"
                     /translation="MEKVPITKAGFEKLKKELETLKNVSIPENVRDIEVARAHGDISE
                     NAEYSAAKERQSFLYGRMQELENQLAMSNVISLKGLNTAKVVFGCYVSLQDIDSGEKI
                     KYQLLGPYESDISRNKISVTSPIGKALIGKNVGSEINVKTPGGTRNLEITDISVEEE"
     gene            40664..>40983
                     /locus_tag="PLR13_02140"
     CDS             40664..>40983
                     /locus_tag="PLR13_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18159.1"
                     /translation="MMLDKYSREINYLRVSITDRCNLRCIYCRPKEGVALHGHEDILR
                     YEEMIRVISLAVKMGLIKVRLTGGEPLVRRGFVDFVADLNKIEGLKDISLTTNGILLE
                     QFAQG"
     assembly_gap    40984..41083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            41343..42125
                     /locus_tag="PLR13_02145"
     CDS             41343..42125
                     /locus_tag="PLR13_02145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18160.1"
                     /translation="MFFDQKKTGFPAERLKTPRDDYIFSRQKELVLDLITPLAGERLL
                     DIGCGTGNHLDMFRGKWCSLTGLDSSGANLEIARQKHSERAELILADPEDIPFSDNEF
                     DIVTMIHCLETAVHPQKIIDEAVRVCRGRVFIGCVNNYSFVGTRQRLKEMFGFSFSQK
                     IRFFSLPEIRKMAEVSAGNNAMKWGSVIYFPAMVYDFFEELEEMLPFRKNPFGAFVGL
                     VCPVTYTYRTAQNPVMNSFNIKAETRKTAPEAVRNMLKEADR"
     gene            42122..>43089
                     /gene="amrS"
                     /locus_tag="PLR13_02150"
     CDS             42122..>43089
                     /gene="amrS"
                     /locus_tag="PLR13_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQC18161.1"
                     /translation="MIKEAMLYEKLQDGNVRCNLCAHRCTIHPERQGVCGVRGNRDGV
                     LYSMAYGTLIAQNVDPIEKKPFFHVYPASRSYSIATMGCNFFCDFCQNHDISQAPRKT
                     KMIYGEDSMPSDIVERAKESGAKTIAYTYTEPTVFFELAYDTAKIAAASGLKNVFVTN
                     GFMTPEAIEIIAPYLSAANVDLKSFRDEFYKKRCGARLVPVLDSLKKMKERGIWVEIT
                     TLLIPSLNDSEEEMRDIAQFIAGLGRETPWHISRFHPQYKMQHLPVTPLESLHRAGQI
                     GREAGLKYVYSGNVPGDDAENTRCFHCGKLLIERYGFKIIGTNLQK"
     assembly_gap    43090..43189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            43259..43420
                     /locus_tag="PLR13_02155"
     CDS             43259..43420
                     /locus_tag="PLR13_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18162.1"
                     /translation="MTEEIKQEQPATDPHKQENQPITLKEKIAAAVLIAYVISLIWLA
                     GSKHWFGLW"
     gene            43699..45627
                     /locus_tag="PLR13_02160"
     CDS             43699..45627
                     /locus_tag="PLR13_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC18163.1"
                     /translation="MRFEHLFSPFTINGMTLKNRAVMPPMATGYGNADNTVSERLLAY
                     LERRAQGGTGLIITEVCAVDPRGKGFPNEIGVWNDDFIGALSRIPAALHRHGAKAALQ
                     LHHAGRETFEAVLGKKPEAPSAIPSAVLGQPCEAMSLERIAEIVRAFAQAAGRARQAG
                     FDSVELHGAHGYLLNQFLSPFSNNREDEYGGSEENRMRFILEVIAAARKAVGDDFPLW
                     IRISADEMVRGGYDFSLMRRLAPRMVAAGVDAIHCSVGVYSTPGGLSIASMDTEPGFN
                     LFRARALKEVVSVPIIGVGRINDPAMADQAIARGDADLISFGRQHLTDPDFIEKARRG
                     SLEDIRRCTACNQGCIERLSFEMKSTTCSFNPECGREYKGPVLPAGTPKKVWVIGAGP
                     AGLSAALVLAARGHQVEVFEKESQPGGQLLSASRPPHKAVFMDWVNWALRRLKQWSVP
                     LHCSHPVSADDIKKAAPDAVIVASGAYPVTASIPGLKSELVADARDVLTGNIKLAGPA
                     VVLGAGYVGMETADYLLANGIAVTVLEMQTLPPVNRFSAHGYWLNKRIKDSGGKIIFG
                     ARVLGIENNIVRYVQADKPSEEKASLIVTAMGAKSENTLEDVLNALAVPYRVVGDARS
                     PRRILEAIHEGYKAGEEI"
     gene            45781..46224
                     /locus_tag="PLR13_02165"
     CDS             45781..46224
                     /locus_tag="PLR13_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQC18164.1"
                     /translation="MQRVSDILKSKSKQMWTINPNDTVFEALKLMAEKEIGALVVVDK
                     KGKVAGIITERDYARKIVLKGKTSPKTLVKEIMTPFNKMYTVKPDTSMEGCMVLMTGK
                     HIRHVPVFEGSKFLGIVSIGDVVKSTISEKDVLINQLSNYIAGKY"
     gene            46487..50035
                     /gene="smc"
                     /locus_tag="PLR13_02170"
     CDS             46487..50035
                     /gene="smc"
                     /locus_tag="PLR13_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQC18165.1"
                     /translation="MNLKNLEISGFKSFRDRVVLDFSNGVTGIVGPNGCGKSNIVDAI
                     RWVMGEQRVKALRGKKMDDVVFNGSQDSAPVSMAEVTMTLYANGQSFPGAYSDLNEVS
                     ISRRVVLDGDSEYFINKVPCRLLDIKEFFMGTGVGARTYSLVEQGHVTNLVEAKPEDR
                     RLYIEDAAGVSKYKSRKEAAVRKMDLTKQNILRLNDIIKEVKTQLNTISRQAKKAEQY
                     KKIKQEIREAEITVALQTYVDFMARESDLQQQRTELQQQEALIRADFQAKESMLDEMK
                     VKLLENEELTAKVQQELYEIKNTISIKEKSIEFAQRQVTDMSGRREKDLTEIQVLQTR
                     KADLAAEIENLRKNSAEAENKIVALKAEHESLQQKVQELTEADKALSSQLEEKKILFI
                     DVVTEKAKLHNMISSLSKNIEDLKKREERETRELEEDQKMMADVCARLEAADEALKKE
                     KADIVGLEAKMAEAATELDRFRSDLQFNEEDIARLREDTSVKSTRLISLREFQESSKW
                     SNEGVKTIIENKEHSDKFYGVIADHISVPREYEAAVEAVLGEKLQYVVVQSQEDGVRA
                     IDYLKNYQLGRGSFISVDLRTHDARTYSEAHLNEAKPLLDKVTVRDDFKPIIDCLLGD
                     VLLIPTIENGISLWKKNGFRGTFVTPEGDIISPHGVLTGGSGSAVEKSLLATKREIGE
                     LEAEIAALSLDLETKVGRRTKILATISRWEEESSQMKTEIHALEITVNGRKKDRERFD
                     DDVNRLRQRIAVLEFNRQTLNTEKTEAEEKLRRFNADVLQKDGEEKQINDIIAALNVQ
                     KEQSRVQIDEQERYVTAGKIELASLEEKKEGDVRTISRLQDHIDSMEQEQRTKEEDIV
                     FCEKQIAELTQGIENDQVLLKEIYSAFAAQEALLAEKKAEQNREDAQLKILEGEIREI
                     KKNLDELRQQVNAMEFQSREITLNRENLRKSVAEKHDVDLAGMAADFEKVTEEKLVEI
                     VDALEKNKQMLETFGEVNLLALNEYEELDQRYNFLSTQIADLNASINVLQRTIARINK
                     ISRARFAETFEAVNACFKEVFAQIFPGGRGELLLTDENDMLETGVDIDIQVPGKRKQN
                     VSLLSGGEKSLVAVALIFAILKYRPSPFLVLDEVDAALDDANTNLFNRLIKDVAGKSQ
                     VVMITHNKSTMEVADTLFGVTMQKQGISTLVSVNLN"
     gene            50087..51022
                     /gene="ftsY"
                     /locus_tag="PLR13_02175"
     CDS             50087..51022
                     /gene="ftsY"
                     /locus_tag="PLR13_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQC18166.1"
                     /translation="MKGASMAKSSLFEKLKAGLVKTRTALTKSLDNLVFGKRVLDQEL
                     FEDLEELLISADMGPQFTYDLIDDVKQRVSRNELNNAQEIKKVLQERMTAILQKMEKP
                     LIIPRGKPFVIMTIGVNGSGKTTTIGKLANILKSEGHEILLVAADTFRAAAVEQLEVW
                     GARVGAPVIKHKTNADPAAVVFDAMSKVKAGFNGVVIVDTAGRLHTRVNLMEELKKVK
                     RIINKELPDVPPEILLILDATTGQNAVAQAKTFKEEIGATGIVLTKLDGTSKGGVVVR
                     IASELALPVWYIGVGEGVDDLRVFESSNFTKALLD"
     gene            51050..51694
                     /locus_tag="PLR13_02180"
     CDS             51050..51694
                     /locus_tag="PLR13_02180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQC18167.1"
                     /translation="MGKIYAIGDIHGCLTKLKTLISDIDIDFKKDTLVFIGDYVDRGE
                     FSRDVVDYVSGLQDSHENIVCLLGNHEQMFMNYLEGVDEEIYLRNGGMQTLRSYEIFL
                     SDDIKERKRKMSARHRKFFETLLPYYETEKYIFVHAGLRPGIPVAEQSQNDLLWIRYE
                     FINTEKDFDKTVVFGHTPLMTPLIDKNKIGIDTGAVYGGKLTCIEFPGLKIYQA"
     gene            51697..52599
                     /gene="ftcD"
                     /locus_tag="PLR13_02185"
     CDS             51697..52599
                     /gene="ftcD"
                     /locus_tag="PLR13_02185"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HQC18168.1"
                     /translation="MKIIECVPNFSEGRDDKKILSIVSVFKMFPGVRLADFSGDADHN
                     RSVFTFLGKPDDVLDATLAACGKALELIDMRQQSGVHPRLGAVDVVPFIPLGRAKMKD
                     AVELAHAFGMRFSQRFGVPVYFYGYAARNGRNVELPDVRRGGYERLADKMSSTESLPD
                     AGENQFNARSGATAVGARDLLVAYNINLVSDDLLLARHIASRIREKGGGLKNVRAIGV
                     MLKRRRLAQVSVNLTDCKETPLKTVFDRVKALAAEGGAQILESELIGLAPKCAFAGAT
                     PEYLRLKDFDENRLLETHIRRLSE"
     gene            complement(52601..52882)
                     /locus_tag="PLR13_02190"
     CDS             complement(52601..52882)
                     /locus_tag="PLR13_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18169.1"
                     /translation="MNDLLAYISNWSVLKWVVLVLIAGFIGQFGRMAAEAIAKKIRAG
                     RPQQENKTIKPETPLQTPAPQPVSSGDLPDKKLLKAIAKAKKKEAKKKE"
     gene            complement(52893..53507)
                     /locus_tag="PLR13_02195"
     CDS             complement(52893..53507)
                     /locus_tag="PLR13_02195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQC18170.1"
                     /translation="MKLFLFDFDGVLVDSLDVYEKTVKDNLQKIKQPVVQSREEFLEL
                     FEDNFYTSLKNKGIDLNVFMKAAEDILAQVRITDMKPFPDVAPVIETLHKNHCLIVVS
                     SNDYASIKEALDLFHFNDYFRDILGSDFMFSKKEKILYAAEKYHVEPNKIYYIGDTTG
                     DIKEGKQAGVKTVGVTWGWHSRERMAAAEPDYLFDTPGELLQLS"
     gene            complement(53504..54553)
                     /gene="mtnA"
                     /locus_tag="PLR13_02200"
     CDS             complement(53504..54553)
                     /gene="mtnA"
                     /locus_tag="PLR13_02200"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQC18171.1"
                     /translation="MIKTIFWKNNAVVLIDQKALPLAEKYIVCKSYKAVIACIKDLTV
                     RGAPAIGVAAAMGAALGAIHLASLSPDKFRKKFYAICDEIAQARPTARNLFWALERMK
                     KIVSHSSQLSQRDIVKELIKKAKHICSEDIAINQQMGKNGSRIIADGDNILTHCNAGA
                     LATAGYGTALGVIRAARKQGKKLHVYVDETRPVLQGARLTAWELKKEKIPYTLITDNM
                     AGFLMQQKKIDKIIVGADRIAANGDTANKIGTYSLAVLAQAHGVPLYIAAPLSTIDVS
                     LKTGDSIPIEERKSDEVAHLQGTRSAPRGTIVYNPAFDITPARLITAMITEKGILRKP
                     FRASIARLYKGERGS"
     gene            complement(54546..55988)
                     /gene="gatB"
                     /locus_tag="PLR13_02205"
     CDS             complement(54546..55988)
                     /gene="gatB"
                     /locus_tag="PLR13_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQC18172.1"
                     /translation="MDFETVIGLEVHAQMLTDTKIFCNCSTKFGAAPNSHTCPVCLGM
                     PGVLPVLNKKVVEYALKMALATNCEINKSCEFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYVMLETNGEQKKIGITRIHMEEDAGKLLHDEHNPVSYVDLNRTGVPLIEIVSEPDMR
                     SPEEAADYLKRLHEILVYLEICDGNMEEGSFRCDANVSIRPKGQKAFGTRTELKNMNS
                     FRNVQRALEYEIKRQQYLVENGGTVIQETRLWDDAQGATNSMRSKEEAHDYRYFPDPD
                     LVPILVDEAWVEKIRKELPELPLAKRERFIKDYQIPAYDAGVLTADKALALYYEKVVK
                     LCNKPKIASNWLMGDVMRFLNEDKRSVRECPIKAESLADMIRLIEEGAISGKMAKEVV
                     EDMYKTGKSPQNIIKEKGLVQITDRDALVKTASITVGKYPQQRDDYRGGGREKLFGFF
                     VGQVMKETKGKANPQLVNEILKEILDKKDD"
     gene            complement(55988..57445)
                     /gene="gatA"
                     /locus_tag="PLR13_02210"
     CDS             complement(55988..57445)
                     /gene="gatA"
                     /locus_tag="PLR13_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQC18173.1"
                     /translation="MKLNQLTIHELQKRIKTGETSATQITHDVFARINAVDEKVHAYI
                     RLMQEEAFQAASKADEDIKQGDIRALTGIPIALKDIVCTKGIPTTCGSRILHNFIPPY
                     DATVVERLRKAGAIFVGKANMDEFAMGSSTETSYFGATRNPWDLERIPGGSSGGSAAA
                     VAADECIASIGSDTGGSIRQPAALCGTVGMKPTYGRVSRFGLIAFASSLDQIGPFTKD
                     VEDCAIMMNVLAGYDPKESTSVNEEVPDYRQFVGRDIKGWKIGIPKEYFIDGIDPGVH
                     AAIKKAITVIEQSGGQCVDISLPHTQYCVATYYIIAPAEASSNLARYDGVRYGLRAQD
                     VRDLLMMYKESRKQGFGAEVKRRIMIGTYALSAGYYDAYYKKASQVRALIKKDFDKAF
                     KICDVIMTPTSPTPAFKIGEKTDDPLQMYLSDIFTISANLAGIPGISVPCGFSSSGLP
                     IGVQFLASHFEEGKLIQIASAYEKNAKIEKRRPAL"
     gene            complement(57507..57794)
                     /gene="gatC"
                     /locus_tag="PLR13_02215"
     CDS             complement(57507..57794)
                     /gene="gatC"
                     /locus_tag="PLR13_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQC18174.1"
                     /translation="MKISSREVEYVAHLARLEITDAEKEKFTAQLNDILLYIDKLNEL
                     DTGDVKPISHAISITNAFREDKISNSIGTREALANAPDTRGEFFRVPKVID"
     gene            complement(57810..58868)
                     /locus_tag="PLR13_02220"
     CDS             complement(57810..58868)
                     /locus_tag="PLR13_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HQC18175.1"
                     /translation="MSRKTGVVKDGKYMQHSMGFAHPESPERLAVIYEMLENPLMSWK
                     FTPIEPREATHEEIERIHARSYVDFIASTAGKRSVFLDPDTSTSPETYDAAKLAAGGV
                     CNAIDSVMKGDVDNAFAFVRPPGHHAEKDTAAGFCVFNNIAIGAMHAIQKHNLKRILI
                     VDWDLHHGNGTQHSFYGDQRVLYFSTHQYPYYPGTGSISETGRGQGEYYTINVPLTMS
                     VGDAVFVKIFRKILEPVALEFKPELILLSAGFDTYYQDPLGAMQVTPEGYAAMTRILL
                     NIADTCCQGRFVAVLEGGYHLSGLVKSVKAVLNEMLDETHFSEERLSLMEKDVDERTT
                     ESVIDKVITRINPYWKVF"
     gene            59105..59365
                     /gene="tatB"
                     /locus_tag="PLR13_02225"
     CDS             59105..59365
                     /gene="tatB"
                     /locus_tag="PLR13_02225"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HQC18176.1"
                     /translation="MFGIGMQELIIIAIIALLVVGPKKLPDLAKSLGKGFSEFRRAAD
                     GVTEDIKDAVKVDEKPKNDDEWKNSLLMKKPDHDNVKSDSSE"
     gene            59427..60191
                     /gene="tatC"
                     /locus_tag="PLR13_02230"
     CDS             59427..60191
                     /gene="tatC"
                     /locus_tag="PLR13_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQC18177.1"
                     /translation="MDNTQAAEDQKMSLTDHLLELRKRLTRSLIALAAGFGVCYYFKD
                     LIFDVMTRPLTQVLSKDSHLVYIGLTEAFFTYMKIAFFASLIITSPYILYQVWKFLSP
                     GLLPKEKQYVVPFVISSSLLFIGGVLFGYFIVLPTAFKFFVSFNNEYLQAMLSFKDYL
                     SLFVTFLLGFGLSFELPIFIFFLTKLGIVNAAKLSRQRRYAILVIFLVAAVLTPSPDA
                     LSQILMAIPLMILYEISIFVSKFAETKKEESQNDEG"
     gene            60200..62224
                     /locus_tag="PLR13_02235"
     CDS             60200..62224
                     /locus_tag="PLR13_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HQC18178.1"
                     /translation="MFLNRKKSNRKNDGEAKKLSSLFNFFLGFVVFILVMTVFGVIGF
                     YAFTMDLPGIDALKDYRPSISSRVYDDKNELIDEFFLEDRKLIKIKEVPRVVVQAFVA
                     AEDSRFFAHKGFDLQSIFRAVFKNIEAGHIIQGGSTITQQVAKMMYLSPEKKYTRKFK
                     EAILAYKIDRYLTKDEILNLYLNQIYLGHGTYGIESASLGYFGKSARELKLHEAALLA
                     GMPKAPSNYSPFLHYDKAKQRQYYVLTRMKEDGYISQEEMEKAYAAPMNLRSIRPKDK
                     MAAYFVETVRRYVQEKYGADVLYKEGLSIYTTLDLTAQKYAGEAMERGLKELEERNKY
                     KSGLVQGALFCMDVRTGAIRAMVGGRDFNQSEFNRATQSRRQAGSAFKPIIYTAAFDK
                     GMTPATVIDDSPLAVDDVSQPDGIWRPKNFDDKFMGPITMRTALVLSRNVVTVKILQE
                     IGIDYAVSYAMNMGITSPLVRTLSLALGASGVTLQELVQAYGVLANQGQKVSPFFIKK
                     IVDRTGNVFEETKVQPEQVIDPRIAFITTHVMQDVVTSGTGTRVRSIGRPVAAKTGTT
                     NDTRDAWFIGMTPSLITGVWIGFDQEASLGSQEVGGRAAAPIWLYFMEKALQNTPVES
                     FPVPEGIVFEKINPKTGEPAGPSEKGTNEAFLLGTSPSSTLGDSIKGLFR"
     gene            62806..64509
                     /gene="ilvB"
                     /locus_tag="PLR13_02240"
     CDS             62806..64509
                     /gene="ilvB"
                     /locus_tag="PLR13_02240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQC18179.1"
                     /translation="MKLKGTEILLKALEEEKVEIIFGYPGGAVLDIYDQIYKSNMKHI
                     LVRHEQGAVHAADGYARSTGKVGVCLVTSGPGATNTVTGIATANMDSIPLVVFTGQVP
                     TALIGNDAFQECDITGITRPCTKHNFLVRNIDDLAATIKEAFDIARSGRPGPVLVDLP
                     KNVMAAQTEYDPANIKIRNHEAAYKPAPKKMAGLLDLLTAAKKPLIMTGGGVILGRAS
                     SLLTEFARKYQMPVTGTLMGLGSFPGTDPLWLGMLGMHGTYYANMAISHCDLLIAVGV
                     RFDDRVTGTIDTFAAKAKIVQIDIDPSSINKNVAVDLPIIGDTQAVLKDLITFLDEHN
                     YAYDKDARNEWLNEIAGWKEKVPLTYCQNGNVIKPQSVIETLYKLTGGDAIITTEVGQ
                     NQMWTAQFFPFDKPNTFISSGGLGTMGFGFPAAIGVKCAFPDKHVVDIAGDGSIQMNI
                     QELATAAQYKINVKIVLLNNGYLGMVRQWQELFYEKRYSFTDMTYAPDFVKLAEAFGV
                     VGLRATKPEDVEPVLKEGLAIDKPVLMDFRVSREECVYPMVHPGDSISNMSLGSREMI
                     N"
     gene            64678..65163
                     /gene="ilvN"
                     /locus_tag="PLR13_02245"
     CDS             64678..65163
                     /gene="ilvN"
                     /locus_tag="PLR13_02245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQC18180.1"
                     /translation="MEKKEHIISILVHNKPDVLARIAGILGGKGYNIESLCVNSTTHY
                     EVSKIVMTTVGTQDTINRIEGQLRRLVDVIQVDDLTNVESIHRELILVRLNLAADKKE
                     QIKKAVDMNKWKVIVNADTYVILEITGDRSQIDFALARLEPLGIADITRTGIVALNLE
                     N"
     gene            65195..66253
                     /gene="ilvC"
                     /locus_tag="PLR13_02250"
     CDS             65195..66253
                     /gene="ilvC"
                     /locus_tag="PLR13_02250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQC18181.1"
                     /translation="MAKINFGGVLEDVITAKEFPLKKAQKVLKNDVVAVLGYGVQGPA
                     QAANMKDNGINVIIGQAKEDTRYWKKAIADGWKPGKTLFPIEEAVKRGTIIQYLVSDA
                     AQKLLWPKVKANLKEGDALYFSHGFSIVYRKQTGVIPPKFVDVIMVAPKGSGTSVRRN
                     FLDGSGINSSFAVQQDYTGRAMERTLALGIAIGSGFLFPTTFQNEVYSDLTGERGVLM
                     GALAGIMEAQYNELRKHRHTPSEAFNETVEELTQSLIRLVAENGMDWMYANCSTTAQR
                     GALDWRHKFRKAVAPVFAELYKSVASGKETAIVLKANSAPDYRVKLEKELKQMRDSEM
                     WKAGAAVRELRPEKRKKK"
     gene            66352..68013
                     /gene="ilvD"
                     /locus_tag="PLR13_02255"
     CDS             66352..68013
                     /gene="ilvD"
                     /locus_tag="PLR13_02255"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQC18182.1"
                     /translation="MRSDLMKKGFERAPHRSLFKAMGYTDEELARPLIGVVNSANEII
                     PGHIHLDLITQDVKAGIRMAGGTPVEFPVIGVCDGIAMGHEGMKFSLASRELIADSIE
                     IMAMAHPFDALVFIPNCDKIIPGMLMAALRLNIPSIFISGGPMLAGKFQDKTVDLISV
                     FEGVGAVRAKKITAAELKQLEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGNGTIP
                     AVLAARHRLAKRAGMKIMDLLKKNIRPRNIATLAAFKNAIAVDMALGCSTNTVLHIPA
                     VAHEAGIKLDLDLFNRISEKTPNLCKLSPAGRHHIEDLDAAGGIQAVMKVISALGVID
                     EKAITVTGKTVGGNLKDARVLNTDVIRTLKNAYSQQGGIAILRGNLAPDGAVVKQSAV
                     APAMQKNEGKARVFDCEDDAIKAILGGKIKGGDIVVIRYEGPKGGPGMREMLSPTSAI
                     VGMGLDKTVALLTDGRFSGGTQGAAIGHISPEAAEGGPIALVKEGDIIAIDIPAKKLN
                     LKVGAQELAKRRKALKPFKPRITTGYLARYAKMVTSSSTGAIFKD"
BASE COUNT        18462 a        15056 c        18033 g        16692 t
ORIGIN      
        1 agcgccggac tccaaatccg gatgctgggg gttcaagtcc ctcctggcct gccatttttc
       61 cccgttgtgt gaaggatgag tcagatggaa gattttaaag aaaaagtaaa agtaacgatt
      121 caaaagatta cacagtttct caaagatgcc aagatggagt tgaaaaaagt tacctggccg
      181 actccaaaac aggccctggc gtctaccgcg gtggtgatca ttctggtttt tattgtttct
      241 ttaattctgg gcattattga ttttgccctc gctaaagcag tgaaattaat attgggttag
      301 gaaaatggct tttaaatggt acgttgtaca tacttattcc ggatacgaga ataaagtcaa
      361 aataagtctt gaagaaagaa ttaagacgac cggaactcag gcgtattttt ccgacattct
      421 gattcctgaa gaagatgttg tggaattgat ttcgggagag aaaaaaagtt cgaaacgcaa
      481 gtttttcccg gggtatatcc ttgtgcggat ggatatgaat gatgaagcct ggcacgtggt
      541 caaggatact cccaaagtga caggattcat cggcagcaag gataaacctt ctcccatacc
      601 tgataaagac gttgaacatc ttaaaatcag aattgatgaa ggaacgttaa aacccaaacc
      661 caaattcaaa ttcgatgtcg gggatcatgt caaaattatc gacggtcctt ttacgaattt
      721 cgacggcatt atcgatgaat taaaaccgga aaaaagtaag cttagggtta tcgtaagtat
      781 ttttggccgg ccgacgccag tggagctgga tttcatccag gtgacgcagg ggtaatgctt
      841 tgcataggcc gaataaataa tatttaaaat tcagatcagg gtgattcgat atggcaaaaa
      901 aaattattgc gaacattaaa ttacagatca aggcgggcaa ggcaacccct tcgcctccga
      961 tcggtccggc attaggtcag cacggcgtga atatcatgga attctgcaag gcttataacg
     1021 cgatgacgca gagccaggaa ggaatgatta ttcctgttgt gattacggtt tatgccgatc
     1081 gctcttttac gtttattaca aaaacacctc ctgtgtcggt gttactgaag caggcagcaa
     1141 aaattgccaa gggatccagc gatcccaaac gggataaagt gggttcggtg accgcatctc
     1201 aggtaaagga tattgccgaa ctgaagtata aggatttaaa cgccgttaat attgaaggcg
     1261 cgataaatat cattgccggc acggcaaggt caatgggaat agaaattaca ggttaataaa
     1321 taaagaggtt tgatcatgtt aagaagaggc aaacgtatat tggcggcaaa ggctaaagtc
     1381 gagccggcca agaaatacac gttgaaagag gctgcggaaa ttgttgtatc gacggcggga
     1441 acgaaatttg atgagaccgt ggatgcggcg attcgtttgg gtgttaatcc cgctcacgcc
     1501 gatcaaatgg tgcggggcag cgtggttctg ccgcacggat tgggtaaaac agtcagggtc
     1561 ctggtatttg ccaaaggtga aaaggaaaaa gaagctttgg aggccggtgc cgatctggtg
     1621 ggcaatgacg aagttatcga gaaaatcaaa ggcggatgga tggaatttga tcgggttatc
     1681 gccacgccgg atatgatggg atcagtcggt aaaatcggta aaattctcgg acctcgcggt
     1741 ttgatgccca accccaaggt ggggacggta acgtttgacg tggccaatgc ggtgaaagaa
     1801 ctcaaagcgg gtaaagtcga gttccgggtc gaaaaagccg gcattgttca ttctccggtg
     1861 ggaaaggtat ccttcggtgc tgaaaaattg agagaaaatg ttagtgcctt gctggagacc
     1921 attcttaaat taaaaccggc atcaagcaag ggtacgtata tcaaaagcat ttcgatctcc
     1981 agcacgatgg gtgtcggggt gagaattgat ccgctcgata ttaaagcagc ttaggatatc
     2041 gggcattttt tagttggtta caaggtcgaa gacagcaggt acagacgttt aaacctgaaa
     2101 aggcctgccg agactcgtaa aataaaaatt gttacttgat ttgtaagggt agactttttt
     2161 tgtaaaacgg gaatcgggat gtaggctggg ttaccgtttt tttgttgtga gactcaaatt
     2221 tcaaaaacga atgcgctaca gggcttcata ggtgtattga aatttgtaag tcgaacaatc
     2281 ccggtatcgg ggttctgtat gtggcttaat tctccggcct cagcaatata ataattttga
     2341 aaggaggctg atagattgga tcggaaaacg aaagaacaaa ttgtatccga actccaaaag
     2401 aaactcaaag aagctaattt aggtattctc accagcttta gcgggatgaa tgtcgaaaaa
     2461 atggaagctt tgagaaatga attgcgcaag tccaatgccg aatggaaggt cgtaaagaat
     2521 acacttttac gcattgcttc cgaaggcacc gatttcagca tacttgccga tcatttcaaa
     2581 tggcccgttg ccattgtcct gagctacaaa gatccggtgg cgccggcgaa ggttctgatt
     2641 gattttgcca agaaaaacgc ggaactggaa atcaaggttg gtgtgctgga taaaaagctt
     2701 ttaaccagga acgacctgac ggttctggca gaattgcccg gcaaagacgt ccttctgggc
     2761 aaactggttt cggtcatggc ggctgttccg acatccttcg tgagagtttt aaatggagta
     2821 ccgggaagtt tcgtgcaagt gcttaacgct tattgcgata agaaaaaatc tttaaactaa
     2881 tacaacatac agaaaggaat aaaacatgtc agataaaatt acgaaagaag atgtggtcaa
     2941 atttattgaa gaaatgacgg tattggaact ttccgctctg atcaaagagc tggaggataa
     3001 atttggtgtt tccgctgccg ctccggttat ggccgccgtg gctgccccgg gtgccgccgc
     3061 tgctgcaggt ccggttgaag aaaaaacaga attcgatgtc attctgaccg gctttggcgc
     3121 ggagaagatt caggtcatca aggttgtccg tgccattacc aacctgggac tcaaagaagc
     3181 caaggatctg gtggaaggcg ttcccaagcc catcaaggaa ggtgcgtcca aggatgaagc
     3241 tgcggacatc aagaagaaaa tcgaagaagt gggcggtacc gtcgagatta aataacgcaa
     3301 tctcaaacat atattattca tttttaagga tgccatggga gactttagaa aaaattttgg
     3361 tcaggtaaga aagatactgg atatccccaa tttaatcgat attcagacag agtcttacga
     3421 gaaatttctc cagaaggata ttgcaccggt gaagcggggc gctttcggtt tgcagggtgc
     3481 tttcaagagc gtttttccta tttcggattt cagcgggaaa tgttctctgg aatttgtgag
     3541 ctataaactg ggtgatatcc ggtatgatgt gaaagaatgt gttcaaaaag gaatgaccta
     3601 cgcggctccc ttgaagatcg tggtccggtt ggttgtgttt gaggccgatc gccccgccga
     3661 agggaaaggt gccggtgaaa cgaccgaagc caagcagatc cgcgatatca aggaacagga
     3721 agtttacttc ggcgaaattc cgttgatgac ggaaaacggt accttcatca ttaacgggac
     3781 ggaacgggtg atcgtcaatc agctgcaccg ttcgccgggt atcttttttg atcacgacaa
     3841 aggtaaaatt gttgccagcg gtaaactgat ttattccgca agagtcatcc ccatcagagg
     3901 atcgtggctg gatctggagt ttgattccaa agatattctt tatgtccgga tcgaccgtcg
     3961 cagaaaaatg ccggtaacca tcctgctgaa agcgctgggg aattcgacgg aattcatcct
     4021 gaattatttt tataacgtca acacagtgat caatgacaat aaaaagattt attttgccgt
     4081 tgatgaatcg ctggtcggca agaaatcacc agaagatatc aaagactcca agagttccga
     4141 actgattgtc aagaagggaa gaaaactgac caagccgctg atcaagagaa tcatggaggc
     4201 cggtgttaaa cggacggcaa tcaatgaaaa cgaactgatc ggaaaaattc cttctcggga
     4261 tgttcatgat ccggaaacgg gtgagattgt ttttcgttgc aacgaggaac tgccggcgaa
     4321 ctgtctggac attatccgcg aaaagaatat ccgggaattg aaacttattc atattgatga
     4381 agacatggat aacgcttcca tccgggatac gctgctgctg gataagattg ataccaccga
     4441 ggatgcgatc atggagatct atcgccgttt gcgtccgagc aatcctccta cgccggaaac
     4501 agcggacaag ttcttcaaga gcctgttctt tgagccggag acttatgatc tgtccaatgt
     4561 cggacgcgcg aaaatgaatt acaaattgca catgaacgtg ccgacggaca acaccgtgtt
     4621 gagcaacgaa gacatcctgt ccgcagtgaa atatctgatc gacctgaaaa acggcgtcgg
     4681 cgattgcagt gtggacgata ttgatcacct gggaaaccgc cgggtgcgtt cggtaggcga
     4741 attgattgaa aaccagtacc gcatcggtct ggtgcgtatg gaaagagcca tcaaggaaaa
     4801 aatgagtctt caggacatcg agaccatgat gccgcacgac ctgatcaatg ccaaaccggt
     4861 ttctgcggtg gtcaatgaat tcttcaattc cagccagttg tctcagttca tggatcagac
     4921 caatccgctt tcggagatta cccataagcg acgtctgtcg gctctgggtc caggaggctt
     4981 gacccgtgaa cgcgcgggat ttgaagtccg cgatgttcat ccgacacatt atggacgcat
     5041 ttgtccgatt gaaacaccgg aaggtccgaa catcggattg atcgtgtcgt tgagcaccta
     5101 tgccagagtg aatgaattcg gttttattga gacaccgtac cggaaagtgg agaacggccg
     5161 ggtgtccgat gatgttcact ttgtaacggc gattgaggaa gaaaatcaga ttattgcttc
     5221 ggcggaccgt cctctgagta aaaacggaaa atttgccgaa gaattgattt cggtcagaaa
     5281 aggcggcagc tatatttcgg ttgagccgga agagattaag atgatggacg tttcgcccac
     5341 ccagctggtc agcgtagctg cagcgttgat tccttttctg gaacatgatg acgccaaccg
     5401 tgcgttgatg ggatcgaata tgcaacggca ggcggttccc ctgatgaatc cggaagttcc
     5461 ggttgtgggg acaggcgtgg aatccgtggt agcccgcgat tccggcgctg tggtcctggc
     5521 caaacggtcc ggtgttgtcg aaagcgttga tgcttcacgc attattgtgc gggccgaccg
     5581 gatggatgaa gacggcatcg acagcggtgt ggatatttat aatctggcga aataccagcg
     5641 atcgaatcag gatacctgtt tcaatcaaaa accgatcatc agcgtcgggc agcgtgtcca
     5701 taaaaaggat atcctcgcgg acggaccggc cattgataac ggcgaactgg cactgggtag
     5761 aaatgtaatg gtcgctttca tgtcctgggg cgggtacaac tatgaagact ccattctggt
     5821 cagcgagagg gtcgtcaagg aagatatttt cacgtcgatt catatcgagg aattcgagac
     5881 catgtcccgc gacacgaaac tgggcaaaga ggaaattacg cgcgatatcc ccaacgtcgg
     5941 cgaagaggtt ctcaaaaacc tggatgaaag cggtattgtt tgcattggcg tttcggtcaa
     6001 acctggggat attctggtcg gcaaaataac gcccaagggc gaaacacagc tgtccgctga
     6061 ggaaaaactt ctgcgggcga ttttcggaga gaaagccagc gatgtccggg atacatcgtt
     6121 gcgcgttccg ccgggcgtgg aaggaacggt tattgacgcc aaaattttta ccagaaaagg
     6181 cgcggaaagc gattcccgtt cacaggccat tgaggcggat gccattgagc agatcgcgaa
     6241 ggacagagat gatgaaatcc gcattctcac cgaaaccatc aaaaaagata ttaccaaact
     6301 tgttgtcggc aaaacggcgg caaccagaat tggggatgcc aaaaagaaaa ccgttctgaa
     6361 gaagggcgac aaaattacga aagagatctg gaacgatctg cctttctcca gctggaaaga
     6421 tattaccatc gccgagaacg acggttcgga agaaaagatc gccggtctga tcgcttcact
     6481 ggaacgaaag atcgattttg tcagtgctca ttttgaggag aagatggaaa agatcaaggc
     6541 cagtgatgaa cttccgcccg gtgtaatcaa gatggtcaag gtgtatattg ctattaaaag
     6601 aaggctgtcc gtgggcgaca agatggcagg gcgccatggg aacaaaggcg ttttatcacg
     6661 gatcctgccg gaagaagaca tgccgtattt tgccgacggt tccacagtgg acatcgtctt
     6721 aaatccgctg ggtgttcctt cgcgaatgaa cgtggggcag attctggaaa cgcatctggg
     6781 ctgggcggca aaatcgatcg gtgagggttt gaatgaactg ctggaacagc agcgcgccgg
     6841 tttgagtcat atcaaagacg agttgaaaaa ggtttactct tcgcccgatt ttgacaaatt
     6901 tatcgatcag gcgtccacgg aagatatcaa acgatttgtc ggccgtttga agaaaggcat
     6961 gttggttgcc agtccggttt ttgacggatg tccggaagat caaatcagag agatgttgaa
     7021 aagaaccgat ttgccggaga ccggccagtc cattcttttt gacggcagaa ccggcgagcc
     7081 ctttgatcag gaggtgacgg taggcattat ttacatgatg aagctgcacc acctggtgga
     7141 caacaagatc cacgcgcgct ccatcgggcc ctattcgctg gtgacccagc aaccgctggg
     7201 cggcaaggcg cagttcggcg gccagcgact gggcgaaatg gaagtctggg ccatggaggc
     7261 ttatggcgcc gcatatgctt tacaggaatt tctgaccgtc aaatcggatg atgtggcggg
     7321 cagaacaaga atatacgaat ccattgtcaa gggtgaacat aattttgagt cgggcctgcc
     7381 ggagtccttc aacgttttgg tgaaggagct tcaggggctt ggattggatg tggaattagt
     7441 cgaagagtcg gatagcctaa gttagcctag gggtatcgcg gcaaaaaaag caacaacagg
     7501 cggcttaata atttcacacc gttatttacg tacaggagaa aatttgtgga agacgttttc
     7561 agctattttg agaaaccgaa gaatttggtt aaattcaacg caatgcagat ttccattgct
     7621 tcaccggata aaattctgtc ctggtcccat ggtgaggtga aaaaaccgga aaccatcaat
     7681 taccgaacat ttaagccgga aaaagacggt ctcttctgcg cgaagatttt cggtccggtc
     7741 aaggattacg aatgtctttg cggacgttat aagagaatga agcaccgcgg cgtggtctgt
     7801 gaaaagtgcg gggttgaggt cattcagtcc aaagttcgcc gcgagcgcat gggccacata
     7861 acgctcgcga cgcccgtcgc tcatatctgg tttttgaaaa gtcttcccag ccgcatcggc
     7921 aacgcggtgg acctttctct taaagaattg gagaaagttc tttactttga atcatggatt
     7981 gttcttgacc ccaagaatac gccattgaag aaaaaagatc tgctttcgga tgaggaatac
     8041 gaagaggcca gggaacagta cggtaatgac ggttttgtcg ccggtatcgg cgccgaggcc
     8101 atcagacaac tgctggaaga aattgatctg gaaaaactct acgaagaact gcgtacggaa
     8161 atcgtgacct ccgtttccga taccaagaaa aagaagctgg tgaagcgctt aaaggttgtt
     8221 gaagcgctgc gcaaatccgg caataagccc gaatggatga ttctcacggt gctgccggtg
     8281 attccgcccg atctgcggcc gctggttcca ctggacggcg gacgttttgc cacatcggat
     8341 ctcaacgatt tatatcgccg cgtcattaac cggaataatc gtctgaagag attgcaggaa
     8401 ctcaacgctc ccgaaatcat cattcgcaat gaaaagagaa tgctgcagga agcggtggac
     8461 gttctttttg acaacggccg acgcggccgt gcgattaccg gaaccaacaa aaggccgttg
     8521 cgctccctgt ccgacatgct caaaggcaag cagggccgtt ttcgtcagaa cctgctgggc
     8581 aagcgcgtcg actactccgg acgttccgtg gttacggtcg gcccggatct gagactgcat
     8641 cagtgcggtt tgccgaagaa aatggccatc gagcttttca agccttttat ctacaatcgg
     8701 ttactggaaa aaggatatgt aaccacggtg aaaagtgcca aaaaaatggt ggaaaaggaa
     8761 tcggcggaag tctgggatgc gctggatgaa gtcgtgcgcg agtatccgat catcttaaac
     8821 cgcgcgccga cgctgcatcg tctgggcatc caggcctttg aaccggtcct gatcgaaggc
     8881 aaggccatcc agcttcaccc gttggtttgt acggcgttca acgcggattt tgacggcgac
     8941 cagatggctg tgcatattcc gctgtccgtt gaagcacaga cggaagcacg cgttctgatg
     9001 atgtccacca ataatattct ttctccggcc aacggcagcc cgatcatcat tcccagccag
     9061 gacatcgttc tgggtattta ttacctgacc cgggccaaga ccggcgtcaa gggcgacggg
     9121 atgatttttg cgggtcccga cgaagtgcgc gccgccattg ataacggcgt gattgacctg
     9181 catgcgcgga tcaaggtgcg cattgattcg gaactgaaag aaaccacggc tggacgaatc
     9241 attctttccg aaatcgtgcc cgagcagatc agcttcgacg atatcaacaa actgatgaac
     9301 aagaaagaac tgaccagcct gatagatcat tgttatcgtc tgtgcggcga caagacaacc
     9361 atcattctgg ccgataaact caaggatctg gggttcaagt atgcgaccgt ttcggggctc
     9421 tcctttgccg tgggcaatat gatcattccg gagcaaaaga aaaaaattgt cctgcaggcc
     9481 gataaagatg ttttgaagat tcaaagacag tacatggacg gcttgattac cgacggagaa
     9541 cgttacaaca aagttgtgga catttgggca caggccactg aaaaaatagc atccgaaatg
     9601 ctcaaaggca tcagtaccga agatgtcccg gccgccgagg gaaagaaacg caaaatcgag
     9661 agtttcaatc ccatttacat gatggctgat tcgggtgccc gcggctccgg acagcagtta
     9721 agacagctgg ccggtatgcg cggtctgatg gcgaagccct ccggtgaaat tatcgaaacg
     9781 cctattacag cgaattttcg cgagggattg acggttcttc agtactttat ttcaacgcac
     9841 ggcgcccgta agggattggc cgatacggcg ctgaagacgg ccaactctgg ttacctgaca
     9901 cgaagactcg tagatgtggc gcaggactgc atcatcacgg agcatgactg cgaaacagtg
     9961 gatggtgtgt atgtatcagc tctgatggaa ggcggcgaaa tcattgaaac cgccggagaa
    10021 cgtgttctgg gaagagttgc gctgcaggat gttaaagatc cgtttagcgg agaagttatc
    10081 gtgccggcca atcagatgat tgatgaaacg ctggctgaaa agatcgacaa tgccggtatc
    10141 gaaaaagtcc atattcggtc ggttctgacc tgtcgttcca agcacggggt ttgtgcgatg
    10201 tgttatggac gtgacctggc gcacggccac ctcgttaata tcggtgaagc ggtcggtatt
    10261 gttgccgctc agtccatcgg cgaaccgggg acccagttga ccatgagaac gtttcatatc
    10321 ggcggtacgg caaaatttga cgaacattcg actctggaag cccgtcacga cggaattgtg
    10381 aaatttgtaa atctcagcac ggtaaaaaca aaggaagggg acctggtcgt gatgaaacgc
    10441 aacggcgaag tccacgtgct ggatgaacag aaacacgttc gcggcaagta cgtcctgtcg
    10501 tacggcgccc acctgaaggt tacggataac agcgaagtga aaaaaggaca gttgattgcc
    10561 gaatgggatc ccttctccat accgattctg gcggaagtgg acggaacgat caagtttgat
    10621 gatatcaccg aaggaaagac catgcaggaa caggttgatg aagtaaccgg tctttcacga
    10681 aagatcatcg ttgagtttaa gggcggggat ttgagacccc gtgtttccat taaggacgaa
    10741 aaaggaaaaa cagccaaact ggcggattcc aatgccgttg cacggtatct gctctcagta
    10801 ggggtcaacc ttgtggtttc tgaaggagat caggtgagag ccggtgatat tatagcgaaa
    10861 attccccggg aaacgacaaa aacgaaagat atcacgggtg gtttgccccg cgttgccgaa
    10921 ctgttcgagg cgcgtaaacc caaagatttc gccgtgatca gtgaaatcag cggtgtggtt
    10981 tcctacggga aagatgccaa agggaaacgt aagatcgttg ttaccccgga agtgggcgag
    11041 gaaaaagagt atcttattcc caaagggaaa catatcagcg ttcaggaggg cgatgttgtt
    11101 tctcccggtg atgccctgat gtccggtgtc aacaatcctc acgatctgct gtccattaag
    11161 ggcgaaaaag aactggcccg ttatctggtg gatgaagttc aggaagtcta ccgtttgcag
    11221 ggcgtgaaaa tcaatgataa acacatggaa gtcattgttc gccagatgct cagacgcgtt
    11281 aaggtaatgg atccgggaga taccgcattt atcgccgacg aaaaaatcga acgataccgc
    11341 ttccaggaag aaaacgaaaa gattattgcc cagggcggcc gacccgctat cggcgagccg
    11401 atgcttctgg ggattaccaa agcatcgctt tccacccaga gctttatttc ggcagcgtcc
    11461 ttccaggaga ccacaaaggt cctgacggaa gccgcccttt ccggtaaaac ggattattta
    11521 agggggctta aggaaaacgt cattatgggc cggttgatta ccgccggaac cggtttggtc
    11581 atgtaccgga agttgggaat tgcgctggaa aatgagaatg ccgagacggc ggcagaataa
    11641 attaaaatat cggcagaaaa tgcttgacat tagtaatttg aactggtacg taccaccggt
    11701 tcgctgccgg ttataataca ttgaagagca gcgatttgtt ttaggaggaa aatgccgaca
    11761 attagtcaac tggttcgcaa gggcagagca aagattaaga gaaaaacaaa ttcacccgcg
    11821 ctggcgggtt cgccgcaacg tcgcggtgtt tgtgttagag tgtatacaac gacaccgaag
    11881 aagccgaatt cggctttaag gaaagtggcc agagtgcgat tgaccagcgg ttatgaagtt
    11941 acgtcataca ttccgggtat cggtcataat ctgcaggagc attcagtggt tctggtccgc
    12001 gggggaaggg tcaaggatct gcccggtgtc cggtatcatg tcatcagagg aactctggac
    12061 acggtcggcg ttcaggatcg caaacagggt cgatcgaagt acggtgctaa aaagccaagt
    12121 taaacggaga ggattcaatg ccgagaagac gggaagttga aaagagagat attttgcccg
    12181 atcccaagtt taataataaa ctggtcgcaa aatttattaa ttcgctgttg aaaagaggta
    12241 agaagagcgt tgcagaaagc attctctacg gcgcttttga tattattgaa aagcgggtca
    12301 aggaacaacc ggtggatttt tttgaaaagg ctgtcaacaa tgtgaaaccg gtgatcgaag
    12361 tcaaatccag gcgtgtcggc ggttctacct atcaggtgcc gacggaagtt gttccggcaa
    12421 gacgcaccgc gctggcgatt cgctggttga tatccaatgc ccaggaacgt acggaaaaaa
    12481 ccatgcggga aaaactggct tcggaattaa tcgatgccgc caacaaccgc gggggagcga
    12541 ttaagaagaa ggaagctgtg cataaaatgg ccgaggcgaa caaagccttt gctcattata
    12601 aattttaaat cataatttta acgataagga aaccaaccat tcaaagttaa ggaggaaagg
    12661 tgaaatggca aagaaaaaat ttgaaaggac taagccgcat ttaaatatag gaacgatcgg
    12721 tcacgtggat catggtaaaa ccacagtaac ggccgcgatt accaaatatc tggcaaaaaa
    12781 gggttttgca gaattcagag ctttcgattc catcgataat gctccggaag aaaaggaacg
    12841 cggcgttacc atcaatattt cccacgtgga atatgagacg gaaaaaagac attacgctca
    12901 tgtggattgc cccggccatg ccgactatat caaaaatatg atttccggcg ccgcccatat
    12961 ggatgggacc attctggtag tggcggcttc cgatggtccg atgcctcaga cacgcgagca
    13021 tatcctcctt gctcgccagg tgcaggttcc ttacattgtc gtttttctga ataaagtgga
    13081 tctggttgat gatcccgagc tgttggacct tgtcgaactt gaattgcgtg aacttttgac
    13141 ccagtatgaa tttcccggtg acaatattcc tatcattcgc ggttcggcac tgaaagcctt
    13201 ggaggccgac gatatcaatt cccctgatgt gaaatgcatt gcggaactga tggatgccgt
    13261 ggataattat gtaccggaac cgaagcgcga tacggacaaa ccgtttctga tgccggtcgg
    13321 tgacgttttc accatttccg gccgcggtac ggttgtgacc ggaagagtag accgcggtat
    13381 tgtcaaggtc ggtgaagaag tggaaatcat cggaatcaga gagaccatta aaacggttgt
    13441 gaccggtgtg gaaatgtttc gcaaaacgct ggatgaaggt cgtgccggtg acgacgtcgg
    13501 cttgttgctc cgcggaatca agcgggaaga agttgaacga ggacaggttg tcgccaagcc
    13561 cgcctccatt acaccgcata ccaaatttga agcggctgtc tatgttctga ccaaggaaga
    13621 aggcggtcgt cataccccgt tcttctccgg ttatcgtccc cagttctact tccggacgac
    13681 ggacgtgacg ggtgttgcaa cgcttcccga gggcgtggaa atggtgatgc ccggcgacaa
    13741 cgtcaaaatg tcaattgaat taattacccc cattgcaatg gaagaacagc tgagattcgc
    13801 tattcgtgaa ggcggcagaa ccgtaggcgc cggagtcgta agcaaggtta ttgcatagaa
    13861 aataggttag ctaacccatg aaagaacaaa agattagaat tcggcttaaa gcttacgatt
    13921 ataaactgtt ggatcgttcc gtcgaggaaa ttgtggaaac ggcgaaaaag accggcgcac
    13981 gtgtcgccgg tcctattcct attcctacgg aaatcaataa gtactgcgtg aatcgctcac
    14041 ctcatgtgga caaaaaatct cgcgagcagt ttgaaatcag aacccacaaa agattggttg
    14101 atatcgtcgg gcctactcaa gccaccgttg acgctttaat gaaactggat ctttcagccg
    14161 gggtggatgt ggaaataaaa gcgtaggcgt gcttttttaa aatgatgaaa aatggcatag
    14221 ggtaatgccg tctttcaaat ggaatatatt attacttaaa aattggaatg tcagatgagc
    14281 aagggaatta ttggtaaaaa actgggaatg acccaagtat ttgcggatga cggatctgcc
    14341 gtcggtgtaa ccgccatcga ggtggaaccc tctgtggttt tacaggtgaa gaccaaagac
    14401 aaggatggct atgatgccgt ccaattgggt tacggccgtg tgaaacagaa aaatgtgacc
    14461 aggccgctgc aaggtcattt taataaggcc aacaagggat ttttccgttt tgtcagagag
    14521 ttcccggcgc attccgaaac gtacgaagtg gggcaggaaa tttctgcgga catatttgcc
    14581 atgggggatt atgtcgacgt tgtcggaaca tcaaagggga aaggttttca gggagttgtg
    14641 aaaagacacg gtttcggtgg cggcagggca acgcatggat ccatgtttca tcgtgcacca
    14701 ggctcgattg gtgccagtgc tgatccctcc cgtgttttca aaggaaccaa gatgggggga
    14761 cacatgggga atgtccgtaa aaccgttcag aatctcaagg tatggcaggt tcgttccgaa
    14821 aggaatctgc ttttgctgga ggggtccgtt ccgggaagta gaaacagttt tttactgatt
    14881 acaaaggcaa agaaaaaata gcgattacgc taaggctgga gttacgcaca tggcagttgc
    14941 agatgtttta aatatcgaaa aaaagaaagt tgccgaggtg gatttgaatg atgccgtctt
    15001 cggggccgac gtcaatgagg cggtaatata cgacgtcgtc aaaatgcagt tggcctcacg
    15061 acgctccggt acggcctcga cgaaaaccag gagtgacgtg agcggcggcg gtaaaaagcc
    15121 gtttcgccag aaaggaaccg gccgtgcaag ggcaggatcg tcccggtcgc ccgtctggag
    15181 aacaggcgga accgttttcg ggccgcatcc gcgggattat tccttcagcg ttcccaaaaa
    15241 aattcgcaag aaagcactga tctcggcgtt gagcctgaaa ttcaaagaaa ataaaatgat
    15301 catcctgaaa gatttcccga tggagaagat cagcgcgcgc ggatttcaga aagtcgtcga
    15361 tcttttcgat ctcaaaaaag cgttatttgt gctggatgat aacaatgaga ttttgatgaa
    15421 atcttcccga aatattaaaa atgtcaagat gatcagatca gagggtatta atgtttatga
    15481 tgtgttgaat catgaacact tgattctgct tgaaccctcc gtaaaaaaga ttgagggggc
    15541 attgctggta taatggacag aagcaaagtc gtgaagagaa tattggttac cgaaaaaagc
    15601 accatggcgc gcgatgcatc caataaatac ttttttgaag tagatcgcaa agccaacaaa
    15661 gtggaaattg ccgatgcggt tgaaaagctt ttcaaggtca aagtaatgga tgtgcgcgtg
    15721 atgcatgttc tgggaaaaaa gaagagaatg ggaaggacag tcggtcaaaa gagctcctgg
    15781 aaaaaggccg tagtgacatt agccgccggc aacagcattg aatttgctga aggcgtataa
    15841 gaaagaatgc attgaaggat tagacggatg gcaattataa agtataaacc gacatcaccg
    15901 ggtagacgat ttcagagcgt ttccgatttt gccgaaatta cctctacgga gccggtgaaa
    15961 aggcttttaa aacctttgaa gaagaaaggc ggccgtaaca gttacggaag agtcacagcc
    16021 cggcatatcg gcggcggtca caaacggaaa taccgggtga ttgatttttg ccgcaataaa
    16081 gccgacattc cggccaaggt ggcttcgatc gaatatgatc caaacagaac ggctcgaatc
    16141 gccctgctca attatcgtga cggtgaaaaa agatacattg tcgcacccgc gcaggtcaac
    16201 gtcggcgatg tcctgtacag cggtgaaaaa gcggatatca aaccgggcaa cgccgtgccc
    16261 ttgaagtata ttccgctggg gtccttgatt cataacgtgg aattgaaagt cggacggggt
    16321 ggccaactga tcagatctgc aggggcctat ggacagctga tggccaaaga gggaagttac
    16381 gctcaggtac gactgccgtc cggtgaagtc cggaaaatct ttattgaatg tatggcgacc
    16441 atcggacagg tcgggaataa cgaacatgaa aacatcagca tcggcaaagc cggaaggaca
    16501 cgctggctgg gtaaaaaacc tcatgtgcgc ggcgtggtca tgaatccggt ggatcatccg
    16561 atgggcggcg gcgaaggcaa atcctccggc gggcgtcatc cgtgcacgcc ctggggtatt
    16621 cccacaaaag gccataagac cagaaagaac aagagaaccg ataagtatat tattaagaga
    16681 cgaggtaaat aagcgtggcg cgttcgatca aaaaaggtcc gtatattgaa gaaaagttac
    16741 tggctaaaat cagaaaaatg gaggccgaag gaaccaagaa tgtcatcaag acttggtcgc
    16801 ggcgttccac tattacaccg gaactgatcg gttatacttt tgccgtacat aatggaaaaa
    16861 aattcatccc ggtttatgtc acggagaata tggtggggca taaactcgga gagtttgcac
    16921 cgaccagggt tttcttttct cattccggtg atcggaaaac caaagtcagc tagtattttc
    16981 gggagataaa gcggtaaagc ttggagttga taaatatgga agcaaaggct gttgcaaaat
    17041 atatgagaat gtcgccgcag aaagttcggc ttgtggcgga tttgatccgg gggaaaaacg
    17101 ttcaggaagc gcgtaacatt cttctctaca ccaggaaata tgcggcaggt gtcgtggcca
    17161 acgtgctgaa atcagctgtg gccaatgctg cccagaatcc caatattgat gaaaatattc
    17221 tttacgtcaa agaaatttat gtcgatcaag ggcccgcctt gaaacgctgg cgcgccagag
    17281 cgcagggaag agcggcaggc atcaagaaac gaagttcaca tattaccgtt attttggacg
    17341 aaatttaagg aggttcagct tggggcagaa ggttaatccg gtaggattac ggttgggcta
    17401 catcaaaaaa tggcaatcgg tctggtatac cgataaaaaa tacagcgacg tgctgcatga
    17461 agatctgcag atcagaaaat atctgaaaaa gaaactgtat catgcgggtc tatccaaaat
    17521 tgaaattgag agagcagcca acaaagcgaa agtcaatatt tactcggcgc gacccggagt
    17581 gatcattggg aaaaaaggat cggagatcga gaagctcaaa tcagaaattg aaagattttc
    17641 gcagtcggaa attatcatta atattctcga agtccgcaag ccggaagtcg atgcgcaact
    17701 ggtggcggaa aacgtggcca tgcaactgga gaaaagggtt gctttccgcc gggcaatgaa
    17761 gaagtgcgtc gggtatgccc tgaagtttgg cgccaagggc atcaaaatcg cctgttcggg
    17821 aagactgggc ggcgcggaaa tgtcgcgatc ggaatggtac cgcgaaggcc gggtgccgct
    17881 gcatacgctg cgggcggata ttgattacgg atttacggaa gccaaaacgg cttacggatt
    17941 gatcggcgta aaagttttta ttttccacgg agaagtttta ccgacgaaga atgaaaaata
    18001 aattgatgtc catcctgggc gctgaacggt ttgtgcggac agggttggat gttgtcagaa
    18061 acaacgggag tttgttttta tgttaatgcc gaaaagggta aaatacagaa agttgcagaa
    18121 aggccggatg ggcggcaagg cgacaagggg cagttccatc gcttttggcg aatacggatt
    18181 gcaggcggcg gagtgcggtt ggattacgtc ccgtcaaatt gaggctgctc gtgttgccat
    18241 gactcgtcac gtcaaacgtg gcggcagaat ctggatcaga gtatttcctc ataagtccgt
    18301 aacaaaaaag cccgctgaaa cccgtatggg caaggggaaa ggcgccccgg aaggatgggt
    18361 ggccgtcgtg aaacccggtt ccgttctcta tgaaatggaa ggcgtttcga aagatgtggc
    18421 ccgcgaggct tttcggctgg cagcccataa attatccatc gcaacaaaat ttttatcgag
    18481 ggatatagtt gatgaaaatt aaggaaataa gagctcttga ctcaaacggg ctcaaacaaa
    18541 aaatcgctga attgagtgaa gaactttttc ggttgaagat ccggcatgca tcggggcaat
    18601 tggaatcaac cgtcatgctg agcaaaatcc ggaaggatat tgcccgtgtc aacacggtgt
    18661 tgaaagaaaa ggaggcagcg agttaacgtg gatcaaagag gatttaatcg caccattaag
    18721 ggcgtggtcg tcagcaataa aatggataaa acaattgtgg tgagagcgga acgttttgta
    18781 aagcatcctg tttttcacaa gtatgtacgt aaacatgtca agtacaaggc gcatgacgag
    18841 cgcaaccaga gcaaaatcgg agatacggtt ttaattattg agtccaggcc gttgagcaga
    18901 gaaaaacgct ggcgaatgct ggaaattttg gagaaagcca aatagattgt ttgacctgat
    18961 ggaataagtc agtttgatga ggatttttat atgattcaga tgcagaccat attaaatgtt
    19021 gcggataatt ccggcgccaa gaaagttgcc tgtatcaagg tgctgggagg atcgaaacgg
    19081 cggtatgcga gtttgggaga cgtgattgtg gtttccgtca aggaagcgat tcccaattcc
    19141 aaagtgaaaa aaggtgatgt gatcaaggcc gttattgtcc gcacggttaa ggaagtgaga
    19201 agaccggacg gctcttattt gaaattcgat gataattcgg cggttttaat ctccaatcag
    19261 atggaacccg tcgggacgcg catttttgga cctgtcgccc gagaattgcg agccagacaa
    19321 tttatgaaaa ttatatctct agctcctgaa gttttgtaaa aatgttttgc agaggggaaa
    19381 aatgagttta aacaaattta aaaaagggga tacggttaag gtccttgccg gtaaagataa
    19441 agggaagacg ggaaaagttc tggccgtcat tcccgagaaa gacaggatcg tggtcgagaa
    19501 agtgaacgtc atcaagaaac ataaaagacc ggaccagcgc tccaaaggcg gtatcgtgga
    19561 aaaagaagga tccattcacg tatccaatgt cggattgttt tgcaataaat gcaataagcc
    19621 cgtcagagtc caaagtaaaa aacttgaaga tggaaagaaa gttcgtatct gcagtaaatg
    19681 caaagacgta atttaatgtg ggttaaataa aatggcaaga ttaaaagaat attattcaaa
    19741 gaccgtagcg cctgcgctgg tgaaggaatt caagtacaag aatcctatgc aggtgcccaa
    19801 gatggagaaa atcgtcatta acatggggtt gggcgaagct attcagaacg tcaagattat
    19861 tgacggtgcc gtacaggaac tggcggccat aaccggccag aaaccgattg tcaacaaagc
    19921 gaagaaatcc attgcgacct tcaagctcag gcaaggcatg ccggtcggct gttccgttac
    19981 gcttcgaaaa aagatcatgt atgaattctt cgatcgtctg gtgaacgctg ctttgccaaa
    20041 ggttcgggat ttccgcggca tatcacccca tgcgtttgac ggcaggggaa atttttccat
    20101 tggcctgcag gaacagatta ttttccccga aattgactat gataaaatcg agaaaaacaa
    20161 aggaatgaat atcaccatcg tgacgacggc gaaaaccgac gaggaagcca ggtttttgct
    20221 gaaaatgatg ggcgttcctt ttaaacagta aattgtgaag ctccggagga attttgcgtg
    20281 gctaaaaagt cattgatcgc gaaagcgaag cgagagaaga aattttcagt cagagaatac
    20341 aatcgatgcc ctctttgcgg aaggccgcgg gcgtattaca gaaaatttga tatgtgcaga
    20401 atttgtttgc gtaaactttc cctgcaggga gaactccccg gggttattaa atccagttgg
    20461 taactttagc ttaaggagaa aaaaagcatg gggatgacgg atcctattgc cgatatgctg
    20521 accagaataa gaaacgccaa taaggcgcgg ttcaagaccg tgcgtgtcag catgtctcag
    20581 gtgaatgtga atgtggccgg tgtattgaaa aaaacaggtt acatcacgaa ttacgaaaag
    20641 gtcaaagatg aaaaaggccg gcagatgctg gaaataattc ttaaatatcc cgatgccaag
    20701 cgtacagtga ttacgaatat taaaagagtc agcaaaccgg gacgcagaat ttatgtgcct
    20761 gcggacaata ttcccaaggt tctcaacggg ttgggaattt ccattctttc gacgtcccgg
    20821 ggggttatca ccgacgcgga agccagagaa ttgaatgtcg gcggggaaat cctctgcaac
    20881 gtttggtaat ttgaacatca agatattagc aggagctgtg gttcatgtcg agaataggta
    20941 aaaaaccagt aagtattccc aagaatgtta aaatcagcca gagtgctgat acggtaaagg
    21001 tcgaggggcc caaaggggcg ctttcttcca gaatgccgga aggaattgcc atcaaaatca
    21061 gcgacggcac actgtcggtt gaaagaaaat cggaagctct gacgacccgt tcctaccacg
    21121 gtttgaccag aacgttgatt gataacatga tcaagggggt taccaccggg tttgaaaagc
    21181 ggctggaaat atctggagtc ggttatcgcg ctgaagcaaa cgataaaagt ttgaaattta
    21241 ttttaggttt ctcgtctccg gttcagtacg atattcccaa gggaatagat atcaaaattg
    21301 ataaacaggt caatatcgtc gtttccggta ttgataaaga gctggtcggc agggttgcgg
    21361 cggaaatcag ggctttgaga aaaccggaac cgtacaaagg caagggcatt aaatatgcga
    21421 atgagtatat caaacgcaaa gccggtaagt ctgccggtgc ttaattggta aaaaaattat
    21481 attgccgtaa agaggtagga aattggcttc gaaaaagaca ttaaagatca gagaaaaaag
    21541 agaaaaaaga acaagatcaa agattaaagg aactcaggag agaccccgtc tttccgtatt
    21601 ccggagtgac aggcatattt atgtgcaggc cgttaatgat gtaacggcag acacgttggc
    21661 aacggcctcg acgctctgca aggaactgga aagcaggctg aaaggcaaaa agaaggtgga
    21721 tgtggccaaa gaagtgggca aactcattgc cgaacgttta aaagctaaag gcattgagaa
    21781 ggtggttttt gatcggggaa gatttctcta tcatgggcgc gtcaaggcat tggccgaagc
    21841 cgccagggaa agcggtctga aattttaatt tattaatcaa tcaagcgaat aaaggaaaga
    21901 atctttggac aagaaagaaa aagaaatgaa agacatggag ctccttgacc gggttgtcgc
    21961 cattaacaga acggcaaaag tggttaaggg cggaaggcgt ttccgtttca gcgccattgt
    22021 tgttgtcggg gacggcaaag gaatgattgg gaccggattg ggaaaagctc atgaagtgcc
    22081 tgaagcgatt cgcaagggca tggatatggc caagaaaaat atggtcaggg tggccgttgt
    22141 ggacagaacc atcccttttg aagtcatcgg ccgctatggt gcggggaagg ttttgctgaa
    22201 gccggcatct gaaggaaccg gattgattgc cggcggcgct gtgcgggccg tgctggaagt
    22261 tgccggtgtg cacaatgttt tgaccaaatg cctgggatct cataatccgc ataacttggt
    22321 aaaagccact ttcgaaggcc tttgtcagtt gaaaaaccct gaagaaattg ccgcacagag
    22381 aggcaaatag ctctgtcaat tatacggtga ggtagcgtat cgtgggtaaa atgttaaaaa
    22441 taacaaaagt gaaaagtgta atcggccgac ccgaaaagca gagaaaaatt ttaaagggca
    22501 tggggctgac caaattaaat aaaaccgtaa ccttggagga tacaccgcag attcgcggga
    22561 tgatcaataa ggttacgcat ttagtttccg tggaagagtc aaaggagtag aatatggatt
    22621 tgggttcgtt gaaggctcct gccggcgcga caaaaaaacg caaacgcgtg gggcggggaa
    22681 atgcatccgg tcacggtgga acatccggca aaggccataa gggacaaaag gctcgttccg
    22741 gcggaaaggt aagggttggt tttgaaggcg gccagatgcc gatggcgcgt cgtcttccca
    22801 aacgagggtt ccgcaatcct ttcagaaaag aatatattgc cgtcaatatt ttggatttgt
    22861 gcagaaaatt ctccaaaggt gcggtgattg acgaggcggc tcttctgcaa agcggcctga
    22921 ttaaaacaga aggcgcccat atcaaaattc tggccaaggg agacattgat tttcctgtga
    22981 cattaaaaat tgcccgaatc agcccggccg caaaagataa aatcatcgct gcgggcggtt
    23041 cggtggaaga ggtaatttga tttgttagaa gggctgggca gtgtatccaa gattccggag
    23101 ttgcaaagaa ggattttatt cacgattctt cttttgggtg tttatcgaat cggcgtttat
    23161 attcccactc cgggaattga taatgtagcg ttactggctt attttgaaca tgccaaagga
    23221 tcgttgttcg gtctgatgga tatgtttgca ggcggagcgt tcagcaattt ttctattttt
    23281 gctctgggca ttatgcccta catcagttct tcgattattc tgcagttgct gacgattgcg
    23341 gttccgcatt tggagcgact gtccaaagaa ggtgaatcgg gccgccgcaa gataacgcaa
    23401 tatacccgtt acgggacggt tcttttaagt atgattcagg ggttcgggat tgcttttggt
    23461 ctgcagcaga tggccgggcc atcgggagct ccgattgttt tgaatccggg tatcggtttt
    23521 gtgctgatga caatgattac gctgacctcc ggtacggcgt ttattatgtg gctgggtgaa
    23581 gtcatcactg aaaagggcat tggcaacggt atatcgctga tcatttttgc cggtatcgtt
    23641 tgccggattc cggcggcaat cggcaatacc ttcaggctgg ccactgccgg tgaactgggc
    23701 gtactggtta ttctcttaat caccgttttg atgatcgcgg tggtaggggc gattgttttt
    23761 gtggaaggcg gtcagcgccg aattccggtt caatatgcaa aaagagtcgt tggccggaaa
    23821 atgtatggcg ggcagacaac ccatctgcct ctgaaagtga atacgtccgg agtaatcccg
    23881 ccgatttttg cttcgtcggt gatcatgttt ccggcaacga tagccaattt tgctccgcag
    23941 acctggatga aggatatcgc cgcctatttg gtgccgggaa gactggcgta cgaagtgctt
    24001 tatgtggcgt ttatcttctt tttctgcttt ttttatacgg cggttacgtt caaaccggac
    24061 gacgttgcgg agaatatgaa aaaatacggc ggttacgtac cgggcatcag accgggaaag
    24121 aaaaccgcgg aatatattga aaacgtactg gaaaaactga ctttcagcgg ggcgatttac
    24181 gtttccatca tctgcgtgct gcccagcgtt ctgattgccc agttaaacgt tcctttttat
    24241 tttgggggaa cggccttgtt gattgttgtc ggggtggcga tggatacggc cagccagatc
    24301 gagtcgcatc ttctgacacg gcaatacgag ggattcatga agaagggcca tattgcccga
    24361 aggagataac ggaagaatat aatggtcatt ctgaagcttc cggaagaaat tgaaagggca
    24421 agagccagta accgaattgt tgccgaggtt ttaagcagac tgagagaaaa ggtgaagccg
    24481 ggtgttaaga ccaaagacct ggataaattt gccgaggaga tcgcggaaaa aagaggagcc
    24541 cggccggcat ttaaaggcta tcatggttat cctcactctt tatgcgtatc gatcaatgaa
    24601 gtggtcgttc acggaatgcc ttccgagcgg gttttggagg aaggagatat tgtcgggctt
    24661 gattttggcg ttttttatca gggatttttc ggtgacgcaa ccgtaaccct gccggtggga
    24721 aaggttacgg caaaagcctt aaggctcatg caggttaccg agcagagctt atacgccggc
    24781 atcgcacagg ccagagaagg caacaggctg ggagatatat ccgctgctgt gcaatctgtg
    24841 gtggaggaag ccggatattc ggtggtccgc gactttgtcg gtcacggcat cggcaaggat
    24901 cttcatgagg agccgcagat tcccaatttt ggccagaagg ggcgcgggat cgaactgaag
    24961 aagggaatga tcctggccat tgagccgatg gtgaatgagg gaaaatacaa ggttcaggtg
    25021 ttgccggacg gctggacggt ggtcacaaaa gacggaagtt tgtcggctca ctttgagcac
    25081 tctattgcga tcacagagca tggtcccgac atattgagcc ggctgaatta ttagctttgg
    25141 aatacaaaag gaattatggc caaagaggaa gcaattgaag tcgaaggaag agtgattgaa
    25201 cccctgccca atgccatgtt ccgggtggaa ctggacaacg gacacaaggt tcttgcgcac
    25261 atttccggaa agatgcgcat gcattttatc aagatcctgc cgggcgacaa ggtgacggtg
    25321 gaactttcac cgtatgattt gacccggggc cggattactt acaggacgaa gtaaaaatga
    25381 ggagtttaaa tcgtgaaggt tagatcatcg gttaaaaaaa tatgcgaaaa atgcaaaatt
    25441 gtcaaacgca aaggtgtttt gcgggtgatt tgcgaaaatc ccaagcataa acaacgtcag
    25501 ggttagtttt tttatattca ggaggtttta ggtggcacga atttcaggtg ttgatttacc
    25561 gaaaaataaa agaatggaaa ttgctttaac gtatatttat ggcattggca agaccagagc
    25621 aaagcaaatc ctgaaagatg ccggggtgag cccggacaca cggacagatg atcttgccga
    25681 ttccgaaata tcggcgatca gaggcatcat cgataaagaa ggcaaagtgg aaggcgatct
    25741 gcgccgggat atatccatgt caatcaaacg tctgatggat gtcggaactt atcgtgggtt
    25801 gagacaccgc aaggggcttc ccgtccgggg ccagagaacg cataccaacg gacgtacccg
    25861 aaaaggtccg cggcgcgcca ttgcgggcaa gaaaaagtaa atttatgatt tccaagtgga
    25921 ggtagaatgg ctaaggcagt cagaaaaacc ggcaagaaaa aagaaaaaaa gaacatcacc
    25981 gacgggattg cccatattca gtcgacgttt aacaatacca ttgtcaccat aaccgatatg
    26041 agtggtaacg tgatcgcatg gtcgtcatcc ggattgcagg gattcaaagg atcgcgcaag
    26101 agcacgcctt ttgccgctca gatggctgcg gaagatgtgg tgcgcaaagc caaagaacag
    26161 ggcatgcgaa aagttcaggt ttatatcaaa ggtcccggcg ccggcagaga atcggcactg
    26221 cgctcgctgc agatcgccgg tctgacgata acgttgattc gcgatgtgac gcccgtgccg
    26281 cataacggat gccggccgcc gaaacgtcgc cgggtgtaac gattaacgaa caatattaat
    26341 ttcaaggaaa tatagggagg caatttttgg caagatatac ggattcctca tgcaggttgt
    26401 gtcgccgcga aggattaaaa cttttttcaa agggtgaccg gtgttactcg gaaaaatgtt
    26461 cctttgaacg aagagattat gttccgggag atcatggtca gatgcataaa aaacagcgtt
    26521 ctgattatgg ggtccagttg cgggaaaaac aaaaactcaa gagaatgtac gggcttttgg
    26581 aaaaacaatt cagaagttat ttcgaaaagg ccgataagca aaagggtatt acaggcacca
    26641 atttgctgat actgctggaa agaagaatgg acaacatggt tttccgcatg ggatttgcca
    26701 attccagaat tgaatcccgg cagttgatca gccacggcca ttttctggtc aacggcaaac
    26761 cggttaacat cccgtcttat ctgctcaagg ccggggatga agtttctgta aaggaaaaca
    26821 gtcgcaaggt gacccgcatc atgggagccc ttgaaacggt tgtcaggcga ggtgttccgc
    26881 aatggctgga attggataaa gacaatttca atgccaagat taaaacactg ccggtgcggg
    26941 aagatttaac catgccgata caggaacagc ttattgtcga gctttattcg aagtaaaaaa
    27001 actattttca taaaccagct actataagga tgggtttaat tatgcaaagg aactggtcaa
    27061 gtttgattcg tcccaagcgt attgatgttg atgaagcgac acatacgaat ttctatggag
    27121 agtttaccat tcagcctctg gaaagaggat ttggtatcac gttgggcaat gccctgagaa
    27181 gggtactgct ttcttccata cagggctctg ccattgtatc agcgaaaatt gaaggggttt
    27241 tgcacgaatt ttcgacaatc tccggtgtta aagaagatgt cacggatatt attttaaacc
    27301 tgaaaggtgt gcgctttaaa ctggggcagg ccaatatcaa ggaagtgagc atcgatgtca
    27361 gccgcgcagg cgaagtgact gctgcggaca ttatgacgga cggcacagtg gaaatcctga
    27421 atcctgagca tcacatcgcc acgctttcca gcggttcttt caaagcgaaa atgatggtaa
    27481 aaatgggcaa gggatacgtc ccggcgaaaa aggaactgga tccggatcag cccaagggaa
    27541 ccatcaatat agacgctgtt ttctcaccca ttaaaaaagt gaattacgtc gtttcctacg
    27601 cccgtgtcgg tcagattgct gattacgaca aactgacctt ggaagtttgg acggacggca
    27661 gcgttaaacc ggaagacgcc atcgcttatt cggcaaaaat actcaaacag caactggatg
    27721 tttttattaa ttttgaagaa gtcgaagagg aaattctccc ggaaaaacag gatgaaccgg
    27781 acaacatcaa caaaattcta tcacggtccg tggaagatct ggaactgtcc gtccgatcgg
    27841 ccaattgtct gaaaaatgcc ggaatcaaca cgatcggaga actggtgaag aaaacagaag
    27901 cggaaatgct taaaaccaag aatttcggca gaaaatcatt gagcgagatc aaagatattc
    27961 tcgcagaata tgatcttacg ttcggaatgg atatcggttc gaaaaattag cattggcggt
    28021 caatatcaaa aatcacgttg tatttattat aaaaggaaag aggtaaatca gcaatgtatc
    28081 acggtaaagc gggcagaaaa ctgggcagga cgtccagcca taaggaagcg atgcttcgca
    28141 atatggtgac ttctgtcatt aaacatgaac gcatccgcac gacagatgcc aaagcaaaag
    28201 aattgagaaa gctggcggag aaaatgatca ccttgggtaa aaaaggcagt ctgcatgcac
    28261 gccgtcaggc actggcggtg atccgggaca aggaaaccgt cggtaagctt ttcggtgaaa
    28321 tgacggaacg ctatcgcaat cgcgccggcg gatatacacg aatagttaaa gccggctacc
    28381 gctatggcga caatgctccg gtttccattc tagaatttat taatgacgag aagaaaaaag
    28441 agaaagcaaa acccaaagcc agatccagag cgaaagcgaa ggaaaaagca gagtaagatt
    28501 ttccatgaat aaaccatcaa aaaggcggcg ggttgtcccc cgcctttttt tgtggttgtt
    28561 ttttgataat aaaagcagta ttgtgaacgc cgaattgaaa cgataaccgc tgcaaagcag
    28621 atgaggcggg taatgggatt taacggcggt gagcttaccg tgatgacact gccgcttgca
    28681 ttaggcagac cgggatcgtt gagtatgatc cagcagacgc aggcgctcaa aacaggaaac
    28741 gaagcagtac tcgatatcat cgattgtccg gtacgggtat atgctttcag ataaactaac
    28801 aatggaggtt tcgttatggc agaaaagaag atcggcgaag taatgaagtt tttctcaaaa
    28861 ccttcagtgg ccgcggtaaa gataaccgat ggcgagattg ccgtcggcga cagcctgaaa
    28921 ttttccggac acaccaccga ttttaccgat gtgattgagt cgatggaggt ggacaatcaa
    28981 tcggtcccaa aagcggtagc cggtgatttt atcggcatca aagtttccga tcgtgtccgg
    29041 cccggcgatg aggttttcaa agtcatcccg gattaatgtt gcccggagat cagcggtttg
    29101 ttatgaaaat cccgattcag gtgaaaagac acagtggtct tttgcccgat caggatttca
    29161 tcgtcgatgc atggttttct tagaggctag caggtcatga aaagcagaat tgccgaagcg
    29221 ataaaactta aataccagcc ggtagcgctg ttgtggtccg ttgaaaaacc tgctgatgcc
    29281 atgcagtttg ccgaagggaa atggggctgc gtcatgtggc tggcggttca cgccgccaaa
    29341 ggtcgtgcgg ctgttgccga caggaaaaca tttggttgtt tcggcggcgg cgtcggtctg
    29401 gggttcggta atcagtacaa aaatttcccc ggcggtgagg agggcttctg ttattttctt
    29461 tccagcggaa atgcccgacg tccgggcgga ccggaaatgg ccgcaaaagt caaaccgttc
    29521 atgaccaggg agagccatga taattttctg aacggcgaac gctacgttca aaatcctcag
    29581 cttgttgaaa aatttatcag ggatcttcct ctgacagata tccccaaacc gtttgttgtt
    29641 tttcaaccgc tgtctgacgt ggatcaggac ggcgaaaagc cgcagaccgt catttttttc
    29701 gtgaatccgg atcagttgtc cgctttaacc gttctggcga actacgggcg tgaaggcaat
    29761 gaaaatgtca tcatccctta cgccgccggt tgccaggcca tcggtattta tccttaccgc
    29821 gaggctgggt tgaaaaaccc ccgtgccgtt gtcggtctta ccgacctttc tgcacgggtt
    29881 tatgttcgca aacaactggg cgagtctcat ctgatgactt tttccgtccc cttcaatctt
    29941 tatgacgaaa tggagcagaa tgtccccggt tcttttctgg agcgtcacac ctggcagagt
    30001 ctgttgtctg agaaccggta attttatttc attccctgtc cggagagggt atgctagctt
    30061 tgcggagatt caggaaggac agatattagc cgggccgtcg accattccat gaaaaattca
    30121 agaacatctc aatacctgga cagtttgaac atcaataaaa tgcggttcgg tctcggggcg
    30181 atcaccagcg tgctgtcgcg tttgggacat ccacaaaaat catttaaggc ggtgaccatt
    30241 accggaacca acggcaaagg atcaaccgct gcgatgatcg cggccatgct gcacaaagcc
    30301 ggttattgcg tcggactgta cacatcgccg catctgattg acgtccggga acgtattacc
    30361 gttaacggaa ggaaaatatc ccggaaagat ttcgacagaa tcattgtctg cgtcagaaag
    30421 aaagtcgttc agccgttgac gtactttgag tttataacgg cggttgcttt tgaatatttc
    30481 aagacgcgaa aagccgatat tgccgtttta gaagtgggcc tgggcggcag actggatgcg
    30541 accaatgttt gccgaccgct cgtttcggtc atcacaaaca ttgcttttga tcacacggcc
    30601 tatctgggtc gtaccctgac gtcgatagcc gtggaaaaag cgggaatcat caagcctcat
    30661 ggtctgtgcg tcaccggagt cgcccaaaag aaaatcttag gattgttgca ggatatctgt
    30721 ctgcagcgcg gtgcttcctt gtaccgtttg aatcacgatt tcagactcgc cagacaaaaa
    30781 aacggatcgt tgctgtatga aggagtgtac agcgatgtaa aaaatctcac cgtgccgctg
    30841 cggggcgaac atcagttggc caatgccgcc ctggctctgg ccgctgttga aatgtgcgct
    30901 cgaaaaggat ttcccgtctc agaaacggcc gttcgcgccg ggcttcaaaa tgtccgctgg
    30961 gagggccggc tggaggtttt acacataagc ccctgggttc tgctggacgg agcgcataat
    31021 cccgcgggca tcggtgcgtt gtgccggtca ctgaaaaaag atttttcctt tcgtcatctg
    31081 attcttatat tcggcgttct ggcggacaag gattaccgta aaatgcttca gaaaatagtt
    31141 ccgctggctc gggtgatcat tctggcgccg ctcaaggcac aaagagccgc ttccgttgcc
    31201 gcggtcagtc aaacgatacg ctcattgggc cgcatgcccg ttgtctctca aagtgttcat
    31261 caggctctgg aactgggtct gtctctggcc gggagtcgcg acctaatctg cgccaccgga
    31321 tctttttatc ttgccggcga agtaaaacag acgttttcta aattagtctt ctgtgataat
    31381 gaaaaaagca aaaaagtaca gggcaagaaa gtttcatgaa catcaacacg ctgattaacg
    31441 tcaagccgat aaaacacaac ggactggctt ctttctccgg ctgccttgag cctgtcttta
    31501 cgttttgtct ggttgcggtg atcatcatcc tgtggtcatt tcccgcggat tgtgcaggca
    31561 agcaaaaatc ctcggcggga aaagcatcac cttccattca gaaaaaaaat ttcccggcat
    31621 ccgttcacac gaacgaaaag tcccctgtca gcattgaagc cgacgccatg gattacgacc
    31681 atgtcagcga tttataccgt gccaggggcc aggtgtccgt cttttacagc ggcgcttcct
    31741 tatacgccga tgaagtggaa ctggataatc agaatcatgt tgcccgggcg cagggcaaca
    31801 cgcttttgaa aatcggagag gatacgctga agggtgaacg cctatggttc aatattgaag
    31861 ataaaaccgg cgccgcgttc aatgctgacg ctttctatgc cagaaaccat tttcgtgtcc
    31921 gtggcaatat catagagaaa acgggagaaa acacctattt tatcgacaat ccctctgcca
    31981 caacctgtga cggcagtaac ccggattggg aaatcgccgg cagtgaaatg aaggtgacca
    32041 ttgaaggcta cggtttgatg aaaaacgcct gttttaaagt agggagcctc cccctgcttt
    32101 attccccgta tattcttttt ccggctaaaa ccaagcggca aaccggtttt ctgcttccct
    32161 atctggcgta ttcccgcgat aaagacggaa tcgacgtgga gcttcctttt ttctgggccg
    32221 tttcgccttg gatggatgcg acgttttatc aacgtttcat tgaaaaaaga ggatttaaag
    32281 aaggggcgga attcagatat gacttcggca ataaatcctt cggcacgttt tacggcgatt
    32341 atctggagga taccaagcag gtgacggaaa cggaggatga cgctctccga cgggactggc
    32401 agggaatgca caaacgctgg tcttatttcc tgcaccatca gacgaggctc gattcgcagt
    32461 tttatctccg aacggacctg aaaaaggtgt ccgacaagtg gtatttcagg gatttttcgt
    32521 cttataatta ttatcgggag cattttaaca gcacgtccga agaccgtttt cggaatgtgt
    32581 ctttcgaagg cgacaaatcg ctgcgttacc tggattcaac catccgcgtg tataaaggat
    32641 ggtccaagta taatgtgacg ggattaatgc atgcggctga cgattttgcc gctgtcaata
    32701 atgatcagac gctgcaaaga tatccggaaa tcatttttgc cggcgtcaag cagcccttgc
    32761 tgcatacgcc tttttattat gagctggccg gccgttacga ttatttatac aagaaggagg
    32821 gtcataaggg acattttgcc gatttgtcgc catctctctc catgccggtc aatctttccg
    32881 actacgtcag aattatcccc cagtttacgt tcaaagaaac gttctggagc cgggatgatg
    32941 atgaaaacgg cttaaaagag aaaacggctg accgctcggt ttataatgcc tcggtgtctt
    33001 ttggcagcca gcttttccgg gtattccatc tcaacgggaa acactgggaa aaaatcagac
    33061 acgagatcaa accggaaatc atctactcct atatgccttc tatttccgca gaggatgttc
    33121 ccgattatta tctgccggcg ttatcgccat tcggaatgcc ccttgcatcg ttgtccagcg
    33181 atccgctgaa cggacagaat gccgttgcct ggtcgctgac caacacactg acggcgaggg
    33241 tcaaagatca gggcgggtcc ttaagctatc tggagttttt ccggctgaaa gtgtttcagg
    33301 cgtacgatat cgatgaggcc cggagagata tggcgcggaa tgcacccgag aggcggcctt
    33361 tctccgacat gggcattgaa ttcgatttca gccctcataa atatttttct ttccggatga
    33421 gggataaata taatgtttac agcggatgga agcaaaacag ttatgatctg agcctcagag
    33481 actggcgcgg cgacgctctg cttgtagcct accggaatac ggaaggtctg gtggaggaaa
    33541 tcaacctggg tgtgaaggcc gtcattaccg atcatttcta cagcaccttt gttctgaaac
    33601 gcgatttgca ggacgcccgg aaaatagaaa attctctggg atttgtttat cacacgcaat
    33661 gctggagtct gggatttgat ctcagcgata cggatgacga tgtcagattc ctgttcagaa
    33721 tatccctggc cggtttcggc aaaaccgcgg acaaataaaa cgatccggat ccgggccgat
    33781 gttcagaatg gagccggccg gacaatgtat tcaatcaagc aaaacaatct ttaaaaaaac
    33841 tttgtcaggt aactatggat acatttattc tcgtggcgac cagtttcacc attgccgttt
    33901 ctctgatgat taccgggaag acgaacaggc ttcagtccgc ctttgccgct ttatgtgcgg
    33961 cggtttttgt ttcacaggcc gccgtgacgc tggaaaatat tttccattat gaatttctct
    34021 tgaaaataaa atatctgggc agcctggcca ttgccccggc ggcgctgcgg tttttccatc
    34081 atctgaccaa ggggcaatcg attatcctcc ggaatttttt ttcgctcttt gcggttatca
    34141 gtctgtgcgg cgtcgttttt gtttttctgc cgttatccga aaaaacctgt tttcataatg
    34201 tcatcgcggc ttatgtgctg ttctcgttga tgatctgcta tttggccctc cttgggcatg
    34261 tcggaacatt gccgtcaggt acggaaaaaa gacgtttcgg ttacctgctg gttttctgcc
    34321 cggtagcttt gattctgagt tatttggatt tattaaattt ctggggattc agcttgctga
    34381 aaatcaacgg catggtatca tccgcactgc tttattttat tttgctgatt attgcctatc
    34441 cgcaactttt ggaattacat gactttttgg cccggtcgct ggttatcctg ctcagcactc
    34501 ttgccggcgc cgccattttt tattttactg tttcgttttt caacggtgat ccccccctga
    34561 ccggattgct gatggcctct tttctcgtcg tgatttccct gtcgccgatg cgaatgattt
    34621 tcagaaaaat cttcggggtt ttctatcccg acagtaaaga tatttttacg tctctttatg
    34681 aatttgacga aaagctggag agagaaaaag ccctgatgct ggaggagatg gcccccgttc
    34741 tcgcccatga gattcgcaat cctttggggt ccatcaaagg agcggcgcag tacctgaaaa
    34801 cggaggcgtc gacgggggaa cacaaggaac tgttggatgt cattgtggaa ggaacggaca
    34861 gattaaatac cgtggtgagc aaatttctgg attacgcgcg tccttaccag cttcagctca
    34921 aaacacagga tattaacgcc atcattcgcc gggccatttc cgttattgcc gcaaacaaac
    34981 tgttggagca catcaccatc gttgaagacc tggaagataa gctgccgttt gcggccgttg
    35041 acgaacagca gtgtctgcaa gtcgttctga atatcgcttt aaacgcgatc gaagccatgc
    35101 cgcagggagg caccctgacg ttttgcacca gcagccagaa atccgaaacg gccgaagact
    35161 tagcgctcac catccgggac acgggtacgg gaatcgatca aaaatacgtc aaggacattt
    35221 tcaagccgtt tttcacaacc aaggaaaggg gtgtcggtct gggactggcc atatgtcaga
    35281 agatcatcag ggaacacaac ggacgcatca gcgtgcagtc gattgcgtct cagggcacca
    35341 ccgttgtgat tcagctgaaa agatccggtt gagtcatccg ataatccggc ttgattaaaa
    35401 tttcaaagca tactatttgc aaactatgaa aaagatttta atcgttgatg atgaactgaa
    35461 catgcggctg gttttgtcgg ccatgctgaa gaaagaaggt tttgaagtgt cctccgcttc
    35521 caacgggcgg gaagccctgc agatccttca atcgaacaag atggccttgg tcattaccga
    35581 tttgaaaatg ccgggaatgg acggcatgga tctattgacc cagatttctg caaaccatcc
    35641 gcaaattccg gttgtcatga ttaccgcgca cgggacgatt gccactgcgg tggaagcgct
    35701 gaaaaaaggg gcattcgatt atattaccaa acctttcgac cttgatgacc tgaagaatat
    35761 tgtgtctaaa gcgatcagaa ccagcacgtt gaaagaaagt gaattttcca tggcgccttc
    35821 ggaaatcgat cgcctgggca tcatcgggac cagccccgcc atcatggaaa tctttgaggc
    35881 cattcaccgg gtagcgcaaa ctaccaccac ggtgatgatc accggcgaaa cggggacggg
    35941 caaagaactg gtggcggacg ccatccatta taattcgcaa cgcaaaaata atcccctgat
    36001 caagatcaac tgtgcagcca ttgccgaaac gctgatggag agtgaactgt tcggttatga
    36061 aaaaggagcc tataccggtg ccgccgtgac caagccgggc aaattcgagc ttgcccacaa
    36121 aggaacgctt tttcttgatg aagtggcgga aattccgcgc gagatgcagg ttaaactgct
    36181 gcgtgtcctt caggaacagg catttgaaag ggtggggggg ctgcgcacca tcaaggtcga
    36241 tgtgcgcatt attgcggcaa ccaatcaaca tcttctgcgt caggttcaga cgggaaattt
    36301 ccgcgaagat ttgtattacc ggctcaacgt ttttcccatc gatatcccgc cgctgagaga
    36361 aagaaaagac gacattctgt cactcgcgga ttacttcctc gataaattca ataaaaaact
    36421 cgggatctct gtcggcatgg acaatgaggt caaagacatt cttttacgtt atgactggcc
    36481 gggcaacatt cgcgaactgg aaaacctcat cgagaggatg atgctgctgg ccaggggatg
    36541 ccggattatg tccggtgacg tacccgatga cttcaaaaaa tcggttgatc atgtcaatac
    36601 ggctttatcc cccggtataa aaaaaccgtt taaggaatat gtgcgcgatc aggtggaaaa
    36661 tgttgagcgg cagatgattg ccagggcgct tgatgaatcc ggcggcaata tcacgcaggc
    36721 ggcgaaaaaa atgggtttga gccgcaaggg gcttcagttg aaaatgagca agtatcatct
    36781 tcggaaggaa tcaaaataaa tgatcgttca tcgttcctca acagaaaagg aggcaaagaa
    36841 tgaattttaa aatcggactg gttgccatct tcgccctgct ggctttgatc tttctggttc
    36901 agaacatcga agtgatgacg gtacattttc tgttctggca actgtcgatg tcgcgcgcga
    36961 ttttcctgtt ttttatcctg ctgaccggtt ttatcatcgg atggttcctg cacagcttcg
    37021 tgtcgtacag ccaaaagaag aaaaactgac ctgacagagg tctgagatcc cagtcaaagt
    37081 cgggtcggcg gcggacccgt tagtttttca gcccgtcata aatatggaga accgcctgaa
    37141 cgtaagcgtc gctgtggttg tatgcataaa ccgctttata ccggctgcga cgtttatgaa
    37201 cgttcttgcc ggagagtccg tgccggtaaa gatagttttc gacgctgaaa atcgtatctt
    37261 ctatggaaaa aggatcaatg tcgtcgttgt ctccattttc gtcgataaag aaagacagca
    37321 atgacgacgg gatgaactgc ccccacccga tggcaccggc gtacgaactt ttgaaatacc
    37381 agggtgaccg gtcggtcttt tctgaaaatt tatagagcga tgttatttca cgaacggcaa
    37441 atttcttccg gtcggtgaag atgtattggg agaccaggga attaaaagcg taaaattttc
    37501 cgcgataacg gcgttgagca aaattggttt ccatgccgat gatggcggca atcaattcct
    37561 tgtgaatccc gtgcttcgcc tcggcccgtt ctaaaatgcc ggtataatcc tcgatgaatg
    37621 ttttgccctt ttcgattttt gtttccacac cgaagatgga gaagtaccac aggaggctgc
    37681 gtttcttttc actgtctgtt ttcttttccg ccgattttct gaaaaatctg tcgatgttgt
    37741 ggtgaagccg aaacgcataa tggtccatct gatcgtcaaa ccattcgccc ggtacaccgt
    37801 tgtccgccac ctcctgcctc aggtcttcaa ggcccgcgtt gatggccgag tccgggagct
    37861 tccggacctg tatattttcc gaggcacaac cggtaaaaat aaaaacgatg gcggcgataa
    37921 tcagtgcgga cttagcattc atgagacaca acctgttcct tcaaattttg tttttgtttc
    37981 acggtttgat ttctactaaa caataaacgg tattgtcaat ttatttcgcg gtgcattgtt
    38041 aaatttaacc gggcgatgtc tgcccttgtg ctcatgtgaa ggttggtgta taaagtttcc
    38101 aaataatagt ttgtatcgga ctcaagacat gcatctgacc ggggcgatgt ttcattctcg
    38161 taaatatccg acaaaagacc actatccttt tcatttgccg ctgttcaggg caacaaaacg
    38221 gctggtgttc gatacgccgg tgacgctgtt tgtgggcgaa aacggaacgg gaaaatccac
    38281 gctgatagag gcgctggctc aagcctgtca aatccatatc tggcgtgaca cgcagagaac
    38341 cagaccggat tacaatcctt atgaagatca gcttcatcgg tatatgtcgg tcatctgggc
    38401 aaacgagccg gtgcccgggt cttttttggg atccaagaat ttccagtact tcgctgaaaa
    38461 actcgacgaa tgggcgtcag cggatcgcga ccagttgaat tatttcggag ggcgatcgct
    38521 gaccactcag tcgcacggtc agtccctgat gtcttttttt tacagccgtt accggattcc
    38581 gggcctgtat ctgctggatg aaccggaaac cgctctgtcg ccgcagtcac agattctcct
    38641 gctggacatt cttcagcgca tgagcgccgc cggccatgcc caattcatca tcgccacgca
    38701 ttcgccgctt ttactggcct gcccacaggc aacaatttat aatttcgaca gatcgccgtt
    38761 gacgaaaatt gtgtacgaga aaacaaagca ctaccggatt tacaaagatt ttctgctgaa
    38821 cagaagcaac tatctaaagc ccaaagcaaa acatcaacga aaggacacca aatgcttacc
    38881 aaagaagaaa tcattgacga agcaaagcgc ctgaaatttg ccgatatcgg tttcacgggc
    38941 gcggagccgt ttggttcgca gaaagactat ctgctgacgc accaggagga atacggctgg
    39001 gctgaagcga ttggcctggg gctgctggcc ggaaccgacc cccgaaatat tcttgccggc
    39061 gcaaaatcga ttatcgttgt tctggaatca tattttgaag aatcctttcc gcagccgatg
    39121 gagcgtcatt tcggccggtg ctatctcgat gacgaccgga ttacccaaga cggtttggcg
    39181 ctgaaaatta aagcctttag aaaatatttg aaggcaaacg gtgttgattc caaagtgccg
    39241 tttcatctgc cgcaccgttt atcagccgcc cgtgccggct tggggacctt tggcaacaac
    39301 tgcatttttt atgcacggcg cgtcgcccgc ggcagttctt tcgtttttcc aattgccttg
    39361 gttgttgatt gtgaatttac accggatgaa ccatccgtcg caatgggctg tcccgactgg
    39421 tgccgcaacg catgcgtggc ggcctgcccc acccgcgcgc tcaagggcgg aggaaaaatt
    39481 gatccgcgcc gctgcatttc tttcctgacc tattacggct ccggattgac gccgcaggac
    39541 cttcgtgagc ccatggggat gtacatttac ggttgtgacc gttgtcaaaa cgtttgcccg
    39601 cgaaacattc cgtggatggc tgaggaaaag ccggtcaatc aacgggtttc agcaaaggcg
    39661 tcggatttcg gattatcccg tcttctgcat atggatgccg tatattttga aaagaaaata
    39721 tggccgcata tgttttatat gtctccggat gatctctggc gctggaagat gaatgttgct
    39781 cgcgccatgg gcaattctct aaatcccgtt tatgtcgccg atctgatccg ggcgtttcat
    39841 gaaaattccg atgaacgcgt gcgatgcatg gcggcctggg cgctggggcg tatcggcggt
    39901 aatgaggctg tggaggcgct tgggaggttt ttgaaaaaca gcgatggcgt tgtccgggaa
    39961 gaaattgagc aagctatggt atcggcttcc gcaggcgtat aaatgaggaa cgttattctt
    40021 cttcaacgga aatatcggtg atttcgagat tccgtgtgcc gccgggtgtc ttgacgttga
    40081 tttcgctgcc gacattttta ccgatgaggg ctttgccgat tggcgacgtc accgaaatct
    40141 tattccggct gatatcggat tcatacgggc caagcagttg atatttaatt ttttcgccgc
    40201 tatcaatatc ctgcaggctg acataacagc cgaaaacaac cttggcggtg ttcaatcctt
    40261 tgagactgat gacattggac atggccagct ggttttccaa ctcctgcatc ctgccgtaga
    40321 gaaacgattg tctttctttg gcggcagaat attccgcatt ctctgaaatg tcgccgtgcg
    40381 cgcgcgccac ttcgatgtca cggacatttt caggaatgga cacgttcttc agtgtttcaa
    40441 gttctttttt cagcttttca aaacccgctt tggtaatcgg cactttttcc atgatctctc
    40501 cggattgttt tgctcttcct gacgtgatta tttttcaaca acaggcagga tgatacgtcg
    40561 tttttgttta atggagaata gtggcgctgt caagaaacat ttaatggccc tgtgtaaata
    40621 attatgctat ttatcacaca atgaaaaaag gattgtatta aaaatgatgc tcgataaata
    40681 cagtagagag ataaattatt tgcgcgtttc cattaccgac cgctgcaatt tgcggtgcat
    40741 ttactgccgt cccaaggaag gcgttgctct gcacggtcat gaggatattt tacgttatga
    40801 ggaaatgatc cgcgttattt ccctggcggt gaaaatggga ttgatcaagg tgcggttaac
    40861 cggcggcgaa ccgcttgtcc ggcgcggctt tgtcgacttc gttgcggacc tgaataaaat
    40921 tgaaggattg aaagatatca gtcttacgac caacgggata ttactcgaac agtttgcaca
    40981 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagtgatg ccgaactgga
    41101 aaagctgatc tgggatgccg ttctgttgaa accccggcag cacgacattg tctgcacgga
    41161 aaacagtctg cgaaaatgtt cccggaatat gtcggaaatc ggcggttgaa aaatatttcc
    41221 caaaaatata tatttaaaaa ataatggctt tatttccggc ggcagggggc tgtcggaccg
    41281 catctggaaa agacggcaat acatcgttac atcgttgtga aaatctgggt aggatcaaga
    41341 ccatgttttt tgatcaaaag aaaaccggat ttccggctga aagattaaaa acaccgaggg
    41401 acgattacat cttttcgagg cagaaagagc tcgttctgga tctgattact cccctggccg
    41461 gtgaacgtct tctggatatc ggttgcggca ccggcaacca tctggatatg ttccgcggaa
    41521 aatggtgttc tctgacgggc cttgattctt ccggcgcaaa tctggaaatc gcccgtcaaa
    41581 aacacagtga acgtgccgaa ctgattctgg ccgatcctga agacattccc ttctccgaca
    41641 atgagtttga cattgtcacg atgattcatt gtctggaaac cgccgtgcat ccgcaaaaaa
    41701 tcattgacga ggccgtccgg gtctgccgtg gccgtgtttt tatcggttgt gtgaacaatt
    41761 actcttttgt cggaaccaga cagcgcctga aagaaatgtt cggtttttct ttttctcaaa
    41821 aaatccgttt tttcagttta cctgaaatca gaaaaatggc ggaggtctcg gccggcaata
    41881 acgccatgaa atggggaagc gttatttatt ttcccgccat ggtttacgat ttttttgaag
    41941 aacttgagga gatgctgccc ttccggaaaa accccttcgg ggcgtttgtc ggcttggttt
    42001 gtcccgtaac gtatacgtac cggacggcac agaatccggt gatgaattca ttcaatatca
    42061 aggcggaaac acgcaaaacg gctcctgaag ctgtgaggaa catgttgaaa gaggcagacc
    42121 gatgatcaaa gaagccatgc tctacgaaaa acttcaagac ggaaacgtcc gatgcaatct
    42181 ttgcgcgcac cgatgcacga tccatcccga gcgtcagggt gtctgcggtg tacggggcaa
    42241 tcgggacggt gtgctgtatt cgatggctta cgggacattg attgctcaaa atgtcgatcc
    42301 catcgaaaaa aaacctttct ttcatgtata ccctgcctca cggtcttact ccattgccac
    42361 gatgggctgc aattttttct gtgacttttg tcagaatcac gatatttcac aagcgccccg
    42421 aaaaacaaaa atgatttacg gcgaggattc catgccgtca gacatcgtgg agcgcgcgaa
    42481 agaaagcggc gctaaaacga tcgcttatac ctacaccgaa ccgacggtgt tttttgaact
    42541 ggcgtacgat accgcaaaaa tcgcagccgc aagcggcctg aagaatgttt ttgttaccaa
    42601 cggtttcatg acacctgaag ccatcgaaat catcgcgccg tatttatccg cggccaatgt
    42661 ggatttgaag tcttttcgcg acgagtttta taaaaaacga tgcggggcac gtctggttcc
    42721 cgttctggac agtttgaaaa aaatgaaaga aagagggatt tgggtggaaa tcaccacgct
    42781 gcttattccg tctctaaatg acagcgaaga agaaatgaga gatatcgcac aatttattgc
    42841 cggactggga agggaaacgc cctggcatat cagccgcttc catccacagt acaaaatgca
    42901 gcacctgccg gtaacgcctc ttgaatccct tcatcgcgca gggcagatcg gcagggaggc
    42961 cggattaaaa tatgtataca gcggcaacgt tcccggcgat gatgccgaaa acacgcgctg
    43021 ttttcactgc ggcaaactgc tgattgaacg ctacggtttt aaaattatcg gcaccaatct
    43081 gcaaaaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attgacaatg
    43201 atccgcattt ccgtatatat tacgtgaaaa tatcaaaaat atgcttggag gaaaacccat
    43261 gaccgaagag atcaagcagg agcagccggc cactgatcct cataaacaag agaatcagcc
    43321 catcaccctt aaagagaaga tagcggcggc tgttttgatc gcttatgtga tttccctgat
    43381 ctggctggca gggagcaaac actggtttgg cctctggtaa agcttgcaat tcccggcgga
    43441 aaaaatctta atttaagctt tgggcaaaaa acagttgagc ctggaacgta ttttcttttg
    43501 gatcatcgtc ttatcagtaa ggtgatctga gaatgatcaa aatgatacgc acggccggct
    43561 gtttccaccg gttactcttc atattttacc gatttggttt tattcctccg gcaaagggga
    43621 caaaatcgtt acggacattc tcaacgttta aacatttttt acgaacgtct ttcagccata
    43681 tcaaacaagg aggcacgaat gcggtttgag catctctttt caccgtttac cataaacgga
    43741 atgactttga aaaacagggc ggtgatgccg ccgatggcaa ccggttacgg taatgccgac
    43801 aacacggtca gtgaacgttt gctggcttat ctggagcgca gggcacaggg cggaacaggg
    43861 ttgattataa ccgaggtctg cgcggttgat ccgcgcggaa aagggtttcc caatgagatt
    43921 ggtgtctgga acgatgattt catcggcgcg ctgtcacgga ttccggccgc gctgcaccgt
    43981 cacggcgcca aggccgcgct gcaattgcat catgcgggcc gtgaaacgtt tgaagcggtg
    44041 ctcggcaaaa aacccgaggc tccttcggcg atacccagtg ccgtattggg gcagccgtgt
    44101 gaagcgatgt ctctggagcg tattgccgaa atagtccgtg cctttgctca ggcggccgga
    44161 cgcgcccgtc aggccggatt tgattcggtg gaacttcacg gtgcgcacgg ctatctgctg
    44221 aatcagtttc tttcaccgtt ctccaacaac cgtgaagatg agtacggcgg aagcgaggaa
    44281 aaccgcatgc gctttattct ggaagtcatc gcggcggcgc gcaaagcggt gggagacgat
    44341 ttccccctct ggattcgcat atcggcggat gaaatggttc gcggtggata tgatttttct
    44401 ctgatgcgca gattagcccc ccggatggtt gctgcgggtg tggatgccat ccattgttcc
    44461 gtgggggttt attccacgcc gggaggtctg tccatcgcct caatggatac cgaacccgga
    44521 ttcaatctgt tccgggcccg tgcgcttaaa gaggtggtga gcgttccgat catcggcgtg
    44581 ggtcggatca atgatccggc aatggccgat caggccattg cccggggcga tgccgacctg
    44641 atcagtttcg gacgtcagca tcttacggat cccgatttta tcgaaaaagc ccgcagggga
    44701 tctctggagg atatccgccg ctgcacagcc tgtaatcagg ggtgtatcga gcgtttgagc
    44761 ttcgagatga aatccaccac gtgcagtttt aatcctgaat gcggacgcga atacaaagga
    44821 cctgttttgc cggccgggac gccgaaaaaa gtatgggtga tcggcgctgg accggcggga
    44881 ctttccgccg ccctggtgct ggctgcacgg ggacatcagg tggaggtttt tgagaaagaa
    44941 tcccagccgg gcgggcaact gctgtcggcc agccgtccgc cgcacaaagc cgtttttatg
    45001 gattgggtaa actgggccct gcgccgtctg aagcaatggt ccgtgccgtt gcactgctct
    45061 catccggtca gcgccgatga catcaaaaag gcagcgcccg atgccgtgat cgtagcgtcg
    45121 ggcgcttacc cggttaccgc ttccattccc ggtctgaaat ccgaactcgt ggctgatgcg
    45181 cgggatgtgc ttaccggaaa tatcaaactt gccggtccgg ctgttgttct gggagccgga
    45241 tatgtcggca tggaaaccgc tgattacctg ctggccaacg gcattgccgt aacggtgctt
    45301 gaaatgcaga cccttccgcc ggtgaacagg ttttccgcgc acggatactg gctgaacaaa
    45361 cgcatcaaag acagcggggg aaaaataata ttcggcgcca gagtcctcgg gattgaaaac
    45421 aacatcgtgc gatatgttca ggcggacaaa ccaagtgagg aaaaggcatc cctgatcgtg
    45481 accgccatgg gcgcgaaatc ggaaaatact ctggaggatg tgcttaacgc gttggccgtt
    45541 ccttaccggg ttgtaggcga tgcccgcagt ccgcgccgga ttttggaagc cattcatgaa
    45601 ggctacaagg ccggggaaga aatctgacag tgatcgtcct tcggaatcgg caagcctttc
    45661 atcaaaaagc acaagcggat cccgggagga tttacattgt gcttgccata ttcccatttt
    45721 tcttttaaaa taagttcaca gccgagaaaa ataaacagac tttatcagga ggtgtggatt
    45781 atgcagagag tgagcgacat tttaaagagc aagtcgaagc agatgtggac aattaatccg
    45841 aacgatacag tatttgaagc ccttaaactc atggcagaaa aagaaatcgg ggcattggtg
    45901 gtcgtggata agaaaggtaa agttgccggc ataatcacag aacgcgatta cgcgagaaaa
    45961 atcgtcctga aaggaaaaac atctcccaaa acactggtca aagaaatcat gacgcctttt
    46021 aataagatgt ataccgtgaa gccggacacc tcgatggagg gttgcatggt attaatgacg
    46081 ggcaagcata ttcggcatgt tccggttttt gaaggaagca aatttctggg gatagtttct
    46141 attggtgatg ttgtaaaatc gacgatttct gaaaaagatg tactgattaa ccagttgagt
    46201 aactacattg cggggaagta ttaattgcta aatatttcaa taatttaagt ttatgtgttg
    46261 ctttcagggc aggggaacat cccctgcctt ttcttgcttt ttcatggttt ttctctttaa
    46321 tttcgatgaa gtacggaatt tcattaaaaa gctaaaaaaa tcaaagcact acatattgtg
    46381 gtatgaatat attaaaacca cattttattg tattttcact ttacaaaggt atattctttt
    46441 gctaaaattt tgcaaatttt gaacaattaa ataaagctgc ttgtttatga atctgaaaaa
    46501 tttggaaata tcggggttta agtccttcag ggacagggtc gtgctggatt tctccaacgg
    46561 tgttacgggt attgtcggtc ccaacggttg cggcaaatct aatatcgtcg atgccattcg
    46621 ctgggtgatg ggtgagcaga gagtcaaggc actgcgaggc aaaaaaatgg atgatgtggt
    46681 cttcaacggc agccaggatt cggcaccggt cagcatggct gaagtgacga tgacgttgta
    46741 tgccaacggc cagagttttc ccggtgctta ttccgattta aacgaagtct ccatctcccg
    46801 cagagttgtt ctggacggcg atagcgaata ttttattaat aaagttccct gccgtcttct
    46861 cgacatcaag gaattcttca tggggaccgg tgtcggtgcc cggacatatt cgctggttga
    46921 acagggccat gtcaccaatc tggttgaggc taagcctgaa gacagaaggt tgtacatcga
    46981 agacgccgcc ggcgtttcca aatataaaag ccgcaaggaa gcggccgtcc gtaagatgga
    47041 cctgacgaaa cagaacattc tgcggctcaa cgatattatc aaggaagtca aaacgcagct
    47101 taacaccatt tcccgtcagg cgaagaaagc ggaacagtac aaaaaaatca agcaggaaat
    47161 ccgagaagcg gaaataacgg ttgccctgca aacctatgtc gactttatgg ccagggagtc
    47221 ggatctgcag caacagcgca cggaattaca gcagcaggaa gctctcatcc gggcggattt
    47281 ccaagcgaaa gaatcgatgc tggatgaaat gaaagtgaaa cttctggaga atgaggaact
    47341 gacggcgaaa gttcagcagg aactttacga aatcaaaaac accatcagta tcaaggaaaa
    47401 aagtattgag tttgcccaac gtcaggtgac cgatatgtcc ggacgccggg agaaagattt
    47461 gacggaaatt caggtgctgc agacgagaaa agccgattta gccgctgaaa tcgaaaatct
    47521 gcggaaaaat tcggcagaag cggaaaataa aatcgtcgct ttgaaagccg aacacgaatc
    47581 gcttcagcaa aaagtccagg aactcaccga agccgacaag gcattgagca gtcagctgga
    47641 agaaaaaaag attttgttta tcgatgtcgt gacggaaaaa gccaaacttc ataatatgat
    47701 ttccagcctc agcaaaaata tcgaggatct gaagaaacga gaggaaaggg aaacccgcga
    47761 actggaagaa gatcagaaga tgatggccga cgtatgcgcc aggctggagg cggccgacga
    47821 agccttgaaa aaagagaaag cggacattgt cggactggaa gctaaaatgg cggaagcggc
    47881 aaccgaactg gatcgattca gatcggattt acagtttaac gaggaagata ttgcaagact
    47941 cagggaagac accagcgtca aatccacccg actgatttct ctgcgggagt ttcaggagtc
    48001 atccaaatgg agtaacgagg gcgttaaaac gattatcgag aataaagagc acagtgataa
    48061 attttacggc gtgattgccg atcatatcag tgttccccgt gaatatgagg ctgccgtgga
    48121 agccgtcctg ggtgaaaaac tgcagtacgt ggtagtgcaa agtcaggaag acggtgtgcg
    48181 cgctatcgat tacttgaaga attatcagtt gggccggggc agttttattt ccgtcgattt
    48241 gagaacccat gatgccagga cttacagcga agcgcacctc aatgaagcga aacccctgct
    48301 ggacaaggta acagtccgcg atgattttaa acccattatc gactgcctgc tgggtgacgt
    48361 tctgctgatt cccacgattg aaaacggcat ctctctctgg aagaaaaacg gtttccgggg
    48421 gacgtttgtc acgccggagg gcgatatcat cagcccgcac ggtgtgttaa ccggcggcag
    48481 cggttcggcg gtggaaaaaa gcctgctggc gacaaaaaga gaaatcggcg aactggaggc
    48541 agagattgca gccttatctc tcgatctgga aaccaaggtt ggccggagaa ctaaaatctt
    48601 agcgaccatt tcccgctggg aagaagaaag ctctcaaatg aagacggaaa ttcatgcgct
    48661 ggaaattacc gtcaacggca gaaaaaagga tcgcgaacgt tttgacgacg atgtcaacag
    48721 actcagacag agaatcgccg ttctggagtt caacagacaa accctgaata cagaaaagac
    48781 ggaagcggaa gaaaagctcc gaaggttcaa tgcggatgtt ttgcaaaagg acggtgaaga
    48841 aaaacagatc aacgacatca tcgccgcgtt aaacgttcaa aaagaacagt cccgcgtcca
    48901 gattgacgaa caggaacgtt atgtaaccgc cgggaaaata gaactggcct ctctggagga
    48961 gaagaaagaa ggggatgtac ggacgatatc caggctgcag gatcatattg attccatgga
    49021 acaggaacag aggaccaaag aagaggacat cgtgttctgt gaaaagcaga tcgccgaatt
    49081 gacgcaaggc attgagaacg atcaggtttt gttaaaagaa atatacagcg cgtttgcggc
    49141 acaggaagca ttgctggccg agaaaaaagc cgagcaaaac cgtgaagatg cacaactaaa
    49201 gatcctggaa ggtgaaatca gggaaatcaa gaagaatctg gatgaattac gccagcaggt
    49261 caatgcaatg gaatttcaaa gccgggaaat aaccctaaac agagaaaacc tcagaaaaag
    49321 cgtggccgaa aaacatgatg ttgatctggc cggcatggcc gcggattttg aaaaggtcac
    49381 ggaagaaaaa ctggtcgaga tcgttgatgc tctggaaaaa aacaaacaga tgctcgagac
    49441 attcggcgag gtgaatctcc ttgccttgaa tgagtatgag gaactcgacc agcgctacaa
    49501 ttttctgtcg acacagattg ccgatctgaa tgcatccatc aatgttttgc agcggaccat
    49561 tgcccggatt aataaaattt cccgcgccag atttgcggaa acctttgaag ccgtcaacgc
    49621 gtgcttcaaa gaagtcttcg cgcagatttt tcccggcgga cgcggagaac ttcttttgac
    49681 ggacgaaaat gatatgctgg aaacaggcgt tgatattgat attcaggtac caggcaaacg
    49741 caagcagaat gtgagtctgc tttccggcgg tgaaaaatca ctggtggcgg ttgctcttat
    49801 ttttgccatt ttgaaatatc gtccctcgcc gtttctggtg ctggatgaag tcgatgccgc
    49861 cctggatgat gccaatacaa atctgttcaa tcgtctgatc aaggatgtcg ctgggaaatc
    49921 ccaggttgtc atgattacgc ataacaagag cacgatggaa gttgccgaca cgctcttcgg
    49981 cgtgaccatg cagaaacagg ggatatccac tctggtttcc gtcaatctca attaatccgt
    50041 ctctttactt cttacaaaaa agcggttatg aatcaattgt ggcaggatga aaggtgcttc
    50101 tatggctaaa tcgagtcttt ttgaaaaatt gaaagcgggg ctggtcaaga ccagaaccgc
    50161 cctgaccaag agtctggata atctggtgtt cggcaagagg gttctggacc aggaactttt
    50221 cgaagatctg gaggaacttc tgatttcagc ggatatgggg ccgcagttta cctacgatct
    50281 gattgatgac gtcaaacagc gcgtcagccg caatgaattg aataacgctc aggagatcaa
    50341 aaaagtttta caggaacgca tgacggccat tctgcaaaaa atggaaaagc ccctgatcat
    50401 accccgcggg aaaccgtttg tgattatgac catcggcgtt aacggcagcg gcaagaccac
    50461 gaccatcgga aagctggcca atatcctgaa aagtgaaggc catgaaattt tgctggtggc
    50521 tgccgataca tttcgggctg ccgccgtcga acaactcgag gtctggggag cacgcgtggg
    50581 cgctcccgtt attaaacaca aaacaaacgc cgacccggca gcggtcgttt ttgacgcgat
    50641 gtcgaaagtc aaggccggtt tcaacggggt ggtgattgtg gataccgcgg ggcggctgca
    50701 cacccgtgtc aatctgatgg aagaactgaa aaaagtgaaa agaatcatca acaaggaatt
    50761 gccggatgtg ccgccggaga ttctgctgat tctggatgcg accaccggcc agaatgccgt
    50821 ggctcaggcc aaaacgttta aagaagaaat cggcgcgaca ggcatcgtgc tgacaaaact
    50881 ggacggcacc tccaaaggcg gagtcgttgt gcgcattgcc agtgaacttg cattgcccgt
    50941 ctggtatatc ggtgttggtg aaggcgtgga cgatctgcgc gtttttgaaa gcagcaattt
    51001 taccaaagca ctgctggatt aagagccgag gcaacgggag cagatccata tggggaaaat
    51061 ctatgccatc ggcgatattc acggttgtct gacaaagctt aaaacgctga tatcggatat
    51121 tgacatcgat ttcaaaaaag acaccctggt gtttatcggc gattacgtcg accgcggtga
    51181 attcagcagg gacgttgtcg attatgtttc cggcctgcag gattcgcatg aaaacatagt
    51241 ttgtttgctc ggcaatcacg aacaaatgtt tatgaattat ctggagggcg tggatgaaga
    51301 gatttatctg agaaacggcg gcatgcagac cctgcgttcc tatgaaatat ttttgtccga
    51361 tgatatcaag gaaagaaaac gtaaaatgtc cgcaaggcac cggaaattct ttgaaaccct
    51421 tctgccgtat tatgaaaccg aaaaatatat ttttgttcat gccggtctca gaccagggat
    51481 tccggtcgcg gaacagtcgc aaaacgatct attatggatc cggtacgagt ttatcaatac
    51541 agaaaaagac tttgacaaga cggttgtgtt cggtcacacg ccgttaatga cccccctgat
    51601 tgacaaaaac aagatcggca ttgataccgg cgcagtttac ggcgggaaac tgacctgtat
    51661 cgaatttccg ggcttaaaaa tttatcaggc atagacatga agatcattga atgcgtaccg
    51721 aacttcagtg aaggacgcga tgacaaaaaa atcttatcca ttgtttctgt ttttaaaatg
    51781 tttcccggcg tccgtttagc cgatttcagc ggggacgccg atcataacag aagtgttttt
    51841 acttttttgg gaaaacccga tgatgtgctg gacgcgacac tggccgcctg tggaaaggcc
    51901 ctggaattaa tcgatatgcg gcaacagtcg ggcgttcacc cgcggctggg tgccgtcgat
    51961 gtcgttcctt ttattccgct gggccgggca aaaatgaaag atgccgttga actggctcat
    52021 gcgttcggca tgcggttttc ccagcggttc ggcgtgccgg tgtattttta cggttacgct
    52081 gcgcgcaacg gtcggaatgt tgaacttccc gatgtccgcc gcggcggtta tgaaagactg
    52141 gctgataaaa tgtcttctac ggaaagcctt cccgatgcgg gagaaaatca attcaacgcg
    52201 cggtccggcg ccaccgctgt aggggcgcgg gatttattgg ttgcctacaa tatcaatctg
    52261 gtctctgacg atctgcttct ggcgcggcat atcgcctcgc gcatcagaga gaagggcggt
    52321 ggattgaaga atgtgcgggc gatcggagtg atgctgaaaa gacgaagact ggcgcaggtc
    52381 tcggtcaatc tgaccgattg taaggaaaca cccttgaaaa ccgtctttga ccgggtgaaa
    52441 gctctcgcgg ctgaaggcgg tgcgcagatt ttggaatcgg agttaatcgg attggcgccg
    52501 aaatgcgctt ttgccggtgc gacgccagaa tatctcagac tgaaagattt tgatgagaat
    52561 cgtctgctgg aaacacacat caggcgcctg tcggaatagg ttactctttt tttttcgcct
    52621 cctttttttt cgctttggca atcgctttga ggagtttttt gtcgggaagg tcaccggatg
    52681 atacaggctg cggtgccgga gtctgcaagg gagtttccgg ctttattgtt ttgttttcct
    52741 gttggggccg gcctgcacgg atttttttgg cgatggcttc agccgccatt ctgccgaatt
    52801 gtccgataaa tccggcaatc agcacaagca caacccattt cagaacagac cagttgctga
    52861 tatatgccag taaatcattc atgtttaaaa acctagctta gttgcaaaag ttcgccgggc
    52921 gtatcgaaca ggtaatccgg ttcggcagca gccattcttt ctctgctgtg ccatccccag
    52981 gtcactccca cggtttttac cccggcctgc tttccttcct tgatatcgcc ggtcgtatcg
    53041 ccaatataat atattttatt gggttccaca tgatattttt ccgcggcata aagaatcttt
    53101 tcttttttgc tgaacatgaa atcggaaccg agtatgtccc ggaaataatc attgaaatga
    53161 aataaatcaa gggcctcttt gatggaggca taatcattgg aggaaacaac aatcaggcag
    53221 tggttcttgt gcagcgtttc gataaccggc gcaacatccg gaaacggttt catgtccgtg
    53281 atccggacct gcgcgagaat atcctcggcg gctttcataa acacattcag atcaatgccc
    53341 ttgtttttca gcgaggtata gaaattgtct tcaaagagtt ccagaaattc ctcccggctt
    53401 tgtacaacag gttgtttaat tttttgaagg ttgtccttga ccgttttttc ataaacgtca
    53461 agcgagtcga caagcacgcc gtcaaaatca aaaaggaata atttcatgat cccctttctc
    53521 ctttatacag acgggcaatg gatgcgcgga aaggttttct taaaattcct ttctctgtga
    53581 tcatcgccgt gatcagccgg gcgggcgtga tgtcaaatgc cggattataa acgatcgttc
    53641 ccctgggagc gctgcgggtt ccttgaagat gtgccacctc atcgcttttc ctttcttcga
    53701 tgggaatcga gtctcctgtt tttaatgaaa cgtcaatcgt ggaaagcggc gccgcaatgt
    53761 agagaggaac gccgtgagcc tgcgccagga ccgccagaga ataggtgccg attttattgg
    53821 ccgtgtcgcc gttggccgca attctgtctg ccccgacgat aatcttgtct atttttttct
    53881 gctgcatgag aaatcccgcc atattatcgg tgattagtgt gtaagggatt ttttcttttt
    53941 tgagttccca ggcggtaagc cgtgcgccct gcaggacagg tctggtctcg tccacgtaaa
    54001 cgtgcagttt tttgccttgt ttacgggctg cacgaatgac gcccagcgcc gttccgtatc
    54061 cggctgtcgc cagcgcgccg gcattgcagt gcgtcagaat attgtcgccg tcggcaatta
    54121 tccggcttcc gtttttcccc atttgttgat tgatggctat atcttcggag cagatatgct
    54181 tggctttttt gattagttct ttgactatat ctcgttggga tagttgactt gaatgactga
    54241 caattttttt catgcgctcc agcgcccaga aaagattgcg tgccgtgggg cgtgcctggg
    54301 caatctcatc acaaatggcg taaaactttt tccggaattt atccggcgat aaagaggcaa
    54361 gatgaatcgc tcccagagcg gcgcccatcg cagcggcaac gccgatggcg ggagctccgc
    54421 gcacagtcaa atctttaata caggcaatca ctgctttgta tgatttgcag actatatatt
    54481 tctcggcaag cggcagagct ttctggtcga ttaaaacgac cgcattgttt ttccagaaaa
    54541 ttgttttaat catctttctt atcaagaatt tctttcagga tttcattgac aagctgcgga
    54601 ttggctttgc ccttggtctc tttcataacc tgaccgacaa aaaaaccaaa aagtttttcc
    54661 ctgcctccac cgcgataatc atcacgctgc tgcggatatt taccgacggt tatgctggct
    54721 gttttaacca gagcatctct atcggtaatc tgcaccaggc ccttttcctt gatgatattc
    54781 tgcggcgatt tgccggtctt atacatatcc tccacaactt ccttggccat ctttccgctg
    54841 atggcgcctt cttcaatcaa tcgaatcata tcggccagcg attcggcctt gatgggacat
    54901 tcgcggacag agcgtttatc ttcattgaga aaacgcataa cgtctcccat gagccagttg
    54961 ctggcgattt tgggtttgtt gcagagtttg accacctttt cgtaataaag agccagcgcc
    55021 ttgtcggcgg tcagcacgcc ggcatcgtaa gccggtattt gataatcctt gatgaatctt
    55081 tcccgtttgg ccagcggcag ttcgggaagc tcttttctga ttttttccac ccacgcttca
    55141 tcaaccagga tggggacaag atcgggatcg gggaagtaac ggtaatcgtg cgcttcttcc
    55201 ttgctgcgca tcgaattggt ggcgccctgg gcgtcatccc aaagccgggt ttcctgaatg
    55261 actgtgccgc cgttttccac caggtattgc tggcgcttga tttcgtattc cagcgcgcgc
    55321 tgcacattgc ggaaggaatt catgtttttc aattcggtcc gggtgccgaa tgccttctgg
    55381 cctttgggac gaatcgatac gttggcatca cagcggaagg aaccttcttc catgttgccg
    55441 tcgcaaattt ccagatacac tagaatttcg tgcaggcgtt tcagataatc ggctgcttcc
    55501 tccggcgaac gcatatccgg ttcgctgaca atttcaatca gcggaacgcc ggtacggttt
    55561 aaatcgacgt aactgaccgg gttgtgctcg tcatgcagca gtttgccggc gtcttcttcc
    55621 atgtgaatcc gggtgatgcc gatttttttc tgctcgccat tcgtctccag catgacatag
    55681 ccgtgttcgg ccagcggata ggcatactgg gatatctggt accccttggg cagatcgggg
    55741 tagaagtaat tcttgcgcgc aaattcgcat gatttattga tttcgcagtt ggttgccagt
    55801 gccatcttca gggcgtattc cacgactttt ttattcagta ccggcaaaac gccgggcatg
    55861 cccagacaaa ccgggcaggt gtggctgttg ggtgcagcgc cgaatttggt ggagcagtta
    55921 cagaaaattt tcgtgtccgt taacatttgg gcgtgaactt ccagcccgat gactgtttca
    55981 aaatccatta taatgcaggt ctcctctttt caatcttggc gtttttctcg taagcagagg
    56041 cgatttgaat gagtttgcct tcttcaaaat ggctcgccag aaactgcact ccgatcggca
    56101 gaccgctcga actaaatccg cagggaaccg agattcccgg tattcccgcc aggttggccg
    56161 agatggtgaa aatatcggaa agatacattt gcagcggatc atctgttttt tcgccgattt
    56221 taaaagccgg tgtcggggat gtaggcgtca taatcacgtc gcaaatttta aacgctttgt
    56281 cgaaatcctt cttgatcaga gccctcacct gcgaagcttt tttgtaatag gcgtcataat
    56341 atcccgcgga caaggcatag gtgccgatca taattcttct ttttacttcc gcgccgaaac
    56401 cctgcttgcg ggattcttta tacatcatca aaagatcccg gacatcttgt gcccgaagcc
    56461 cgtaccggac gccgtcatag cgcgccagat tggaactggc ttccgccgga gcaataatat
    56521 aataggttgc cacacagtat tgtgtatgag gcagtgaaat atccacgcac tggccaccgc
    56581 tctgttcaat gacggtaatc gctttcttaa tggcggcatg gactcccgga tcaatgccgt
    56641 cgataaaata ttccttcggg atgccgattt tccagccttt gatgtcgcgc ccgacaaatt
    56701 gccgataatc gggaacttcc tcgttcacgg aagtggattc tttcggatca tacccggcca
    56761 gcacattcat catgatggcg cagtcctcga catctttggt aaacggtccg atctgatcaa
    56821 gcgatgaggc aaaagcaatc agaccgaagc gcgacacccg tccgtaggtc ggtttcatgc
    56881 cgacagtacc gcaaagcgcc gccggttgac ggatggagcc gccggtatcg gaaccgatgg
    56941 aggcgatgca ttcatcggcg gcaaccgcgg cggcggaccc gccgctggag cccccgggaa
    57001 tccgttccag atcccatgga ttgcgtgtgg caccgaaata ggaagtttcc gtggacgaac
    57061 ccattgcgaa ttcatccata ttggccttgc cgacaaatat cgctccagcc ttgcgcagtc
    57121 tttccacgac cgttgcatca tacggcggaa taaaattatg cagaatgcgc gatccgcagg
    57181 tggtgggaat gcctttggtg cagacaatgt ctttcagagc gataggaatg ccggttaaag
    57241 ctcttatgtc accttgttta atgtcttcat ccgcttttga cgcggcctga aaagcctctt
    57301 cctgcatcag acgaatatag gcatgaacct tttcgtcgac agcattgatt ctggcaaaaa
    57361 catcatgagt aatttgcgtg gccgatgttt cgccggtttt gattcttttc tgcagttcat
    57421 gaatggttaa ctgattcaac ttcataaaat tcctttatat ttaattaggg cctggaatag
    57481 tcaacgttgt gatttcggac gagattttaa tcgattactt tggggacgcg gaagaactca
    57541 cctcgggtgt ccggcgcatt ggccaatgcc tcccgggtgc cgatggagtt tgatatctta
    57601 tcttcgcgaa acgcattggt tatgctgatt gcatggctta tcggtttcac atccccggta
    57661 tccagttcgt tcaacttatc aatatagagc agaatgtcgt ttaactgagc cgtaaatttc
    57721 tctttttctg cgtccgttat ttccagacgc gccaaatgag ccacatactc aacttcgcgg
    57781 gaacttattt tcaaaaaaca tcatctcctt taaaaaactt tccagtaggg atttattctg
    57841 gtgatcacct tatcgatcac ggattccgtt gttctttcat ccacgtcttt ctccatcaga
    57901 gacaagcgtt cttccgagaa atgggtttca tccagcattt cgttcaagac ggcttttacc
    57961 gacttgacca gtccgctcag atgatatcct ccttccagga cggctacaaa acgtccctga
    58021 cagcaggtgt cggcaatatt gagaaggata cgcgtcatag cggcgtagcc ctccggcgtg
    58081 acctgcattg cgcccagcgg atcctgataa tacgtatcaa aaccggcgga cagcaggatc
    58141 aattcaggtt tgaattccag cgccacgggt tcaagaatct tccggaatat cttgacaaag
    58201 accgcatcgc cgacagacat ggtcaggggg acattgatcg tataatattc gccctggccc
    58261 cggccggttt ccgaaatgct tcccgttccc ggataatagg gatattgatg cgtggaaaaa
    58321 tataaaacgc gctgatcccc gtaaaaactg tgctgggtgc cgttaccgtg atgcagatcc
    58381 cagtcgacaa tcagaattcg ctttaaattg tgtttttgga tggcatgcat ggccccgatg
    58441 gcgatattat tgaaaacgca gaagccggcg gccgtatctt tttccgcatg gtgtcccggc
    58501 ggtctgacaa aagcaaaagc gttatccacg tcgcctttca tgacgctgtc aatggcattg
    58561 cagacaccgc cggcggctag ctttgcggcg tcgtaagttt ccggagatgt cgatgtgtcg
    58621 ggatcgagaa aaacgctgcg cttgcccgcg gtgctggcga taaagtccac ataagagcga
    58681 gcatgaatcc tttcaatttc ttcgtgagtg gcttcacgcg gttcgatcgg cgtgaatttc
    58741 caggacatca gcgggttttc cagcatttcg taaatgacgg cgagcctctc cgggctttcg
    58801 ggatgagcaa aacccatcga atgctgcatg tattttccat ctttgacgac gcctgttttc
    58861 ctggacaagt gaaccttctt cggtaaaaat gccgttaaaa aaagttaatt ccgtttacca
    58921 taaatagttg caaagagcaa aagaaatcac cgctttaaaa caggaaaaga taaaatggac
    58981 gtttttgttc gtttgatcgc tgtttgatga attcggaaaa ttcaaaaaag tctgcgacaa
    59041 aaaacattga caaaaacagc aatcaatata taggaataca aaagttgccc ggagaggata
    59101 cacgatgttt ggcataggga tgcaggaact gataattatt gcgataattg ctcttctggt
    59161 tgtcggtcct aagaaactgc ccgatctggc caagtctctt ggtaaaggct tcagcgaatt
    59221 ccgcagagcg gctgacggag tgactgaaga tatcaaggac gccgtcaaag tggatgaaaa
    59281 accgaagaat gacgacgaat ggaagaattc tcttttaatg aagaagcccg atcatgacaa
    59341 cgtaaaatcc gattcatctg aataaatcca aacgataaac tgattcgctc tgactatcaa
    59401 cgctgcaaaa ggcaaaacgt caaactatgg acaataccca ggccgccgaa gatcaaaaaa
    59461 tgtctctgac cgatcatttg ctggaactgc gcaaaaggct cacccgttct ttaattgcgc
    59521 tggcggccgg tttcggtgtc tgttattatt ttaaagacct gatttttgat gtgatgaccc
    59581 gtccgctgac tcaagtgttg tcgaaagaca gtcatcttgt ctatatcggt ttgacggaag
    59641 cctttttcac gtacatgaaa atcgcttttt ttgcttccct gatcattacc agtccctaca
    59701 ttctgtatca ggtatggaaa ttcctttcac cgggactgct tccgaaagaa aagcagtatg
    59761 ttgtcccgtt tgtcatttct tcatctctgc tttttatcgg aggcgttctc tttgggtatt
    59821 ttatcgtttt gcccacggcg ttcaaatttt ttgtcagttt caataatgaa tacttgcagg
    59881 cgatgctttc gtttaaggat tatttgtctc tttttgtaac gttcctgctc gggttcggtt
    59941 tgtcttttga attgccgatt ttcatttttt ttctgaccaa actggggatc gtcaatgcag
    60001 ccaagctttc cagacagagg cgatacgcga ttcttgttat ctttctggtt gccgcagtcc
    60061 tgacgccttc gccggacgcc ctgagccaga tattaatggc aattcctctc atgatcctgt
    60121 atgaaataag catatttgtt tccaaatttg ccgagacgaa aaaagaagaa tcccaaaatg
    60181 acgaaggata atctgtaaaa tgtttctaaa tcgtaaaaag tcaaacagaa aaaacgacgg
    60241 agaagctaaa aaactttctt cgctttttaa tttttttctg ggctttgtcg tcttcattct
    60301 ggtgatgaca gttttcgggg tcatcggatt ctatgctttt acgatggatt tgcccggtat
    60361 cgatgcgctc aaggattatc gtcccagcat ttcttcccgc gtttatgacg ataaaaacga
    60421 actgatcgat gagttttttc tggaagacag aaaactcatc aagattaagg aagttccgcg
    60481 agttgttgtt caggcttttg tggcggcgga ggattcccgt ttctttgcgc acaagggttt
    60541 cgatctgcaa agcattttcc gggcggtttt caaaaacatc gaggcgggac atatcatcca
    60601 gggcggcagc acgattaccc agcaggttgc caagatgatg tatctttctc ccgaaaagaa
    60661 atatacccgt aaattcaagg aagctattct agcttacaaa attgatcggt acctgaccaa
    60721 agacgagatt ctgaatttat atttaaacca gatttatctg ggccatggta cttacggcat
    60781 agagtccgct tcgcttggtt atttcgggaa gagtgcccgg gaactgaaat tgcacgaagc
    60841 ggctcttctg gccggtatgc ccaaagctcc cagcaattat tcccctttcc ttcattatga
    60901 caaagccaaa caaagacaat attacgttct gacgcgcatg aaagaagacg gctatatttc
    60961 ccaggaggaa atggaaaaag cgtatgctgc gccgatgaat ctgcgatcca tccgccccaa
    61021 ggacaagatg gccgcctatt tcgtcgagac cgttcgacgg tatgttcagg aaaaatacgg
    61081 cgccgatgtt ttgtacaagg aaggtctttc catctatacc acgctggatc tgaccgcgca
    61141 gaagtacgcc ggggaggcga tggagcgggg cttgaaggaa ctggaggaac ggaataaata
    61201 caaatccggt ctcgtgcaag gcgcgctttt ctgcatggac gtcagaacgg gtgccatccg
    61261 tgcgatggtc ggcgggcgcg acttcaatca aagcgagttc aaccgcgcga cgcagtcacg
    61321 acgtcaggcc ggttcagcgt tcaaaccgat catttatacg gcggcttttg ataaaggcat
    61381 gacgcctgca accgttattg acgattcacc gcttgctgtg gatgatgttt cgcagcctga
    61441 cggcatctgg aggccgaaaa actttgacga caaattcatg gggccgatca caatgagaac
    61501 ggcgctcgtg ctgtcccgca atgttgtaac ggtaaaaatt ttgcaggaaa tcggcattga
    61561 ttatgctgtt tcgtacgcca tgaatatggg aattacatcc cctctggtgc gaacactgtc
    61621 tttggcactg ggcgcatccg gggtaacgct gcaggagctg gttcaagcgt atggcgtttt
    61681 ggctaatcag ggacagaaag tttctccttt tttcatcaaa aaaatcgttg atcgcaccgg
    61741 caatgttttt gaagaaacaa aagttcagcc ggaacaggtg attgatcccc gcatcgcatt
    61801 catcacgact catgtcatgc aggatgtcgt aacaagcggc accggtacga gagtcaggag
    61861 catagggcgt ccggtggctg caaaaaccgg aacgaccaac gatacgcgtg atgcctggtt
    61921 tatcgggatg acgccttcgc tgatcaccgg agtgtggatc ggtttcgatc aggaagcctc
    61981 gctgggatca caggaagtcg gcggacgggc ggcggcaccg atctggcttt attttatgga
    62041 aaaggccttg cagaacacac cggtggagtc ttttcccgtt ccggaaggca tcgtctttga
    62101 aaaaatcaat ccgaaaacgg gagagcctgc cggaccttcg gaaaagggga ccaatgaagc
    62161 cttcctgttg ggaacgtctc ccagcagcac tctgggcgat tctattaaag gtttattccg
    62221 gtagccggga gttattcctg gggcgtgtcg acgatccgga aagaatgtcc ggcaaaaaat
    62281 attgttgaca aagacagtgt ttttcgttat tgaaagcacg aaataattca acggaatgat
    62341 gtgtttatgt ttcttaaagt tttacaaagc ggcattatcc gggtactgaa taaagtactg
    62401 gtcctcgtag tagtcgtact tacggggtct gctgctctgt atctttaaga aaccggaata
    62461 aaagatacta gccgcaggcc cctgaagccc gcggctttgt tgtttttggc ataaagccgc
    62521 tttttctcaa ggaaacaggc ggcttttttt tgtgagttcc aaatttcaga agcggagcac
    62581 gctgaaattt gcagaacgaa caatccccgg agcgaagcgg agtgggatcg gagactcaac
    62641 tttcaggaac gcatatcctg aaggtcgcta taggtggaaa tttgtacggc ggattaattc
    62701 tgggcagggt aagcgggtta agtgatcttt aaaaaagcga aagagacatg gcctgaaaca
    62761 agaccgtaaa aaattaatta taaatcattt taaaggggaa aaggaatgaa actgaagggg
    62821 acagaaatat tgctgaaagc gctcgaagag gagaaggttg aaattatctt cgggtatccc
    62881 ggaggcgctg ttctcgatat ttacgatcag atatacaaaa gcaacatgaa acacattctg
    62941 gtccggcatg agcagggagc cgtgcacgca gccgacggct atgcccggtc caccggaaaa
    63001 gtcggcgttt gtctggtgac gtcgggtccc ggcgcaacca acacagtcac cggcattgcc
    63061 acggcgaata tggattccat tcctctggtt gtttttacgg ggcaggttcc cactgcgctg
    63121 atcggcaacg acgcttttca ggaatgcgat atcaccggca taacccgtcc gtgcaccaaa
    63181 cataattttc tggtgcgcaa tattgacgat ctcgctgcga cgatcaagga agcgtttgat
    63241 attgcccgct cgggaagacc cggtccggtg cttgttgatc tgcctaaaaa tgtgatggcg
    63301 gcccagacgg aatatgatcc ggccaacatc aaaatcagaa atcatgaggc cgcgtacaaa
    63361 ccggctccga aaaagatggc cggtttactg gatttgctga cagcggcgaa aaagcctctg
    63421 attatgaccg gcggcggtgt cattctgggc agggcgtcat cgctcctaac ggagtttgcc
    63481 aggaaatatc agatgccggt gaccggaacc ctgatggggc ttggttcttt cccggggact
    63541 gatccgcttt ggctgggcat gctgggcatg cacggaacgt actatgccaa catggcgatc
    63601 agccattgtg atctgctcat tgccgtgggc gtcagatttg acgaccgggt aaccggcacc
    63661 atcgatacgt ttgccgccaa ggccaaaata gttcagattg acattgatcc gtcatccatc
    63721 aataaaaacg ttgccgtgga tctgccgatc atcggcgata cccaggcggt gctgaaggac
    63781 ctcatcacat ttctggacga acacaattac gcttatgaca aggatgcaag aaatgaatgg
    63841 ctgaatgaaa ttgccggctg gaaggaaaaa gtgccgctga cctattgcca gaacggcaac
    63901 gtcatcaaac cgcagtctgt catcgagaca ctgtataagt tgaccggcgg cgacgcgatt
    63961 attacaacgg aagtcggcca gaaccagatg tggaccgcgc agttcttccc gtttgacaag
    64021 cccaatacgt ttatttcatc cggcggactg ggaacaatgg gattcggttt ccccgcggct
    64081 atcggtgtga aatgcgcttt tcccgacaag cacgtggtgg acattgccgg cgacggcagt
    64141 atccagatga atatccagga actggccacc gccgcgcagt ataaaatcaa tgtaaaaatc
    64201 gttctgctga ataacggcta tctgggcatg gtgcgtcagt ggcaggaatt gttttacgaa
    64261 aaacggtatt catttacaga tatgacctat gcgcctgatt ttgtaaaact ggctgaggcg
    64321 ttcggcgtcg tgggcttgcg cgccacaaaa ccggaagacg tggagcctgt gttaaaagaa
    64381 ggactggcta ttgacaaacc ggtgctgatg gattttcgtg tctcccggga agaatgcgtt
    64441 taccccatgg tgcatccggg cgattcgatc agcaacatgt cgctggggag cagagaaatg
    64501 atcaattgac gatttcgtga aaagtccaga tgctgcgttg cgcttcatcc ttcggcgttg
    64561 cggcgtatgt gaaaaatacg cctcacttct caggcttcgc gcgccttgcc tctgtagctt
    64621 ttgacaaagt ttttaaataa gaagtattaa ataataaata atatgatgag ggagaagatg
    64681 gaaaagaagg aacatatcat ttcaatactg gttcacaaca aaccggacgt gctggcgaga
    64741 atcgccggca tcctgggcgg taagggttat aacatcgaaa gcctgtgcgt gaattcaacc
    64801 acacattacg aagtatcaaa aatcgtgatg accaccgtgg gtacgcagga caccatcaac
    64861 agaatcgaag ggcaattgcg aaggctggta gatgtaattc aggtggatga tttgaccaat
    64921 gtggaatcga ttcaccgcga attgatcctc gtgcggttaa atctggcggc cgataaaaaa
    64981 gaacagataa aaaaagctgt tgacatgaat aaatggaagg taatagtcaa tgccgacacc
    65041 tacgtgattc tggaaataac cggtgatcgg tcacagattg attttgcgct ggccagactg
    65101 gagccgctgg gcatagccga tatcacaaga accggcattg tcgctttaaa tttggaaaac
    65161 taaattataa tattttattc aatagggggt taatatggca aaaattaatt ttggcggtgt
    65221 tctcgaagat gttattacgg cgaaggaatt tcctctgaaa aaagctcaga aagttttgaa
    65281 gaatgatgtg gtggcagtgc tggggtacgg cgtgcagggg ccggctcagg cggctaatat
    65341 gaaggataac ggcattaatg tgatcatcgg ccaggccaag gaagatacgc gctactggaa
    65401 aaaggccatt gccgatggat ggaagcccgg caagactctt ttcccgattg aagaagcggt
    65461 caagcgcggt accatcattc agtatctggt ttctgatgcg gcgcagaaac ttctttggcc
    65521 caaggtcaag gctaacctga aagagggcga cgctctatat ttctcacacg gattttccat
    65581 tgtttacaga aagcagaccg gcgttatacc gccaaaattt gttgatgtga tcatggtagc
    65641 tcccaaaggg tcgggaacat ccgtccgccg gaatttcctg gacggcagcg gtatcaactc
    65701 cagctttgcc gtgcagcagg attatacggg acgcgcaatg gaaagaacgc tggccctggg
    65761 catcgccatc ggctccggat ttttattccc gacaaccttc cagaacgagg tttacagcga
    65821 tttgaccggt gaacgcggcg tgcttatggg tgcgctggcg ggcatcatgg aagcccagta
    65881 caacgaactg cgcaaacacc gccatacgcc cagtgaagct tttaacgaaa cggtggaaga
    65941 actgacacag agcctgatcc gtctggttgc agaaaacggt atggactgga tgtatgccaa
    66001 ttgctccacc acggctcagc gcggcgctct ggattggcgt cataaattcc gcaaagcggt
    66061 tgcacccgtg tttgccgagc tttataagtc ggtggcgagt ggcaaggaaa cagccattgt
    66121 cctgaaggcc aacagtgctc ccgattaccg ggtcaaactg gaaaaagaac ttaaacagat
    66181 gcgtgattcg gaaatgtgga aagccggcgc tgccgtgcgt gaattacgac cggaaaaacg
    66241 aaagaagaaa tagacgactc cttaaaaagt tcattttacg ccctctccct tgaggggaga
    66301 ggggcgggga agtttctatt agaaatttat tttatcaagg gagagataac catgcgaagc
    66361 gatctcatga aaaaaggttt cgagcgggcg ccgcatcgct cgttgtttaa ggcaatgggg
    66421 tatacagatg aagagctggc caggccgttg atcggcgtgg tcaattcggc caatgaaatc
    66481 attcccggac atatccatct ggatttgatt acgcaggatg tcaaggcggg aatccggatg
    66541 gccggcggaa caccggtgga gttcccggtc attggcgtct gcgacggcat cgccatgggg
    66601 cacgaaggta tgaaattttc gctggccagt cgcgaactga tcgccgattc catcgagatc
    66661 atggcgatgg ctcatccttt tgacgcgctg gttttcattc ccaactgcga caagattatt
    66721 ccgggcatgc tcatggccgc tctgcgtctg aacattccgt ccatttttat cagcggcggt
    66781 ccgatgctgg cgggaaaatt tcaggacaag accgttgacc tgatcagtgt ttttgaaggc
    66841 gtgggcgccg tcagggcaaa aaaaatcacc gccgctgaac tgaagcaact ggaagactgt
    66901 gcctgccccg gctgcggttc atgttccggc atgtacacgg ccaattccat gaactgcgtc
    66961 accgaagcgc tgggtctcgg cttgcccggc aacggaacga ttcccgccgt cctggccgct
    67021 cgtcaccgcc tggccaaacg cgccggaatg aaaatcatgg atttgctcaa gaaaaatatc
    67081 cggccgcgca acattgccac attggctgca tttaaaaatg ctatcgctgt cgacatggcg
    67141 ctgggctgtt ccaccaacac ggttttgcac attccggccg ttgcccatga agcgggcatc
    67201 aaactcgatc tggatctttt caacaggatc agtgaaaaaa ctcccaatct ctgcaaattg
    67261 agccccgccg ggcgtcatca catcgaagat ctggacgcgg cgggcggcat acaggctgtg
    67321 atgaaagtca tcagcgcgct gggtgtcatt gatgaaaaag ccatcacggt aaccggcaaa
    67381 accgtcggcg ggaatttgaa agatgcccgt gtgttgaata cggatgtcat cagaactctc
    67441 aagaatgctt attctcagca gggcggtatc gcgattctgc gcggcaatct cgcgccggac
    67501 ggagcggtag tgaaacaatc ggcggtcgcg ccggccatgc agaaaaacga aggcaaagcg
    67561 cgtgtgtttg actgcgaaga cgacgccatt aaggcgattc ttggcggaaa aatcaaaggc
    67621 ggcgatatcg ttgtcattcg ttacgaaggt cccaaaggcg gtcccggcat gagggaaatg
    67681 ctttctccca cttcggcaat cgttggcatg ggtctggata aaacggttgc tctcttaacc
    67741 gacggacgtt tcagtggcgg tacacagggc gcagccatcg gccatatctc accagaagca
    67801 gcagaaggcg ggccgatcgc gctggtgaaa gaaggcgaca tcatcgccat cgatataccg
    67861 gccaagaaac tgaacctgaa agtcggtgct caggaactgg ccaaacgcag aaaagcattg
    67921 aaaccattca agccgagaat tacgacgggt tatctggcga ggtatgcgaa aatggtgacg
    67981 tcgtcctcga cgggcgccat cttcaaagac tgataccaat tttgatttct tcggctaact
    68041 ttgttggcag cgtcgtcttc ctcgacgtat tatcatatac gtctgtggag ccgactcctt
    68101 gccgccttga tatgatcttt gatttggaag cggaatacaa aaggtatgtt attgctgggt
    68161 tggaagtcag gcgaatgcct gattacccgg caatttatat ttgtagctca ttcgagtttc
    68221 tttattaaag tacgttcttt aaacattttg agatgattat ttgttattac ttactaacta
    68281 actgggaatt aaattttttc atgagggatg tttcattaag attctcaaaa gtcatcaatg
    68341 tgtaaaaatt agaaactgca cgatcaagaa tgttttgcgc ttcttctttt agattacatt
    68401 gaatatttat atcatctctt cctttcatgt gttttgaacc att
//
LOCUS       DAONQZ010000006        65118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_466_length_65118_cov_19.433103, whole genome shotgun sequence.
ACCESSION   DAONQZ010000006 DAONQZ010000000
VERSION     DAONQZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 65118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65118
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_65118_cov_19.433103"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..2139)
                     /locus_tag="PLR13_02260"
     CDS             complement(172..2139)
                     /locus_tag="PLR13_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HQC18183.1"
                     /translation="MMNHPIKSRHPIASDLARRMKRGKIVSLDEAVGVIRDGDTLAFG
                     GFVGIGFAEGVARAIEKQFIAQGKPKDLTIIYSAGIGDGKDKGLNHLAHEGLVSRVIG
                     GHWGLVPKLQKLAIENKIEAYNLPQGVISHMFRDIAAHNPRTLSKVGLGTFVDPRHGG
                     GKINRKTRNDIVELLNFDGEDCLAYKTRPIQIAVLRGTTADLDGNVTMEREALTVDAL
                     SIAMAVRNSGGVVIVQVERIADRGTLRAREVRIPDILVDCVVVADPDDHWMTFAEKYN
                     PAYSGETRVPVTSLEPLPLDERKIIARRAALELKVNSVVNLGIGVPESIARIAAEEGL
                     LEYLTLTAEPGVIGGIPAGGLSFGAATNVEAIIDQPYQFDFYDGGGLDIAFLGLAQAD
                     RAGNLNVSRFGSRLAGAGGFINISQNAKKVVFAGTFVAGNLQVDVADEKLKIISDGDM
                     PKFIDAVDQITFSGAVGAQSGRTILYVTERCVFRLSEKGLMLVEIAPGVNLQKDILEK
                     MKFTPLMAEKLLMMDARIFRPEAMGLKEDLLTLPLAERFTYQPEENLFFVNFEGLSIR
                     STEQIDEIREHVERICRPLLPKKVQTIVNYDNFAILPELIEPYTAMVDHVVSRYYDKV
                     TRYTTSAFMRVKLGDLLAERSVAPHVFEKGH"
     gene            complement(2166..3962)
                     /locus_tag="PLR13_02265"
     CDS             complement(2166..3962)
                     /locus_tag="PLR13_02265"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HQC18184.1"
                     /translation="MNHYDLFKSWHQLMTQTTDMACNKKIKSEAPVEPLGALASWVDL
                     GTRLSHLTWARFSEDAWEYSIDFWQRCVLFMDTMRRRGNTMLEHYQQGMPPLLDYEFE
                     VVLDARKFPRPVNYSLLYIRHPEGKEPKPGKRPILVIDPRAGHGPGIGGFKKDSEVGM
                     GLAEGHPVYFASFTPEPLPDQTMEDVEQAEIRFLEEIKKRHPDRDFPMIYGNCQAGWA
                     VAMLGADRPDVAGPIVINGAPMSYWAGGDGINPMRLTGGLTGGTWATSFMCDLGGGLF
                     DGAYLVENFERLNPANTLWKKDYNLFSNIDTEQKRYLEFERWWGGFYLLNEEEILWIV
                     RNLFIGNKLEKGMLDIGHGHRIDMRNMKDPLVIFASSGDNITPPQQALHWISEVYPTT
                     DDLVKAGQRIVYLLHSHIGHLGIFVSASVARREHRAIIEHIEKMQKLKPGLYEMIIEG
                     ETGDHDPHQEQFRVRFEKREIKDVTCPIPKSAFDKMDRLSVANENLYLAFGRPFVKSL
                     IRHPQQAAALRWLHPARVSRYVWSDQVSPGMKIFDSWASWVKDNRSRAQESNTFSYIE
                     RRHSDNIATFLDACRDIRDTGLEVIFEAIYRE"
     gene            4329..4679
                     /locus_tag="PLR13_02270"
     CDS             4329..4679
                     /locus_tag="PLR13_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18185.1"
                     /translation="MNNGSTKMTDIKIDTNNLYKEEAFTDLTFATIRRLNPVNLDGSP
                     DASREPIFTGMTQLMSPNGPIPVQCLIEGAKTLAEAAEKLPVAIEKTVQAMIAEAKEM
                     EREEASKIVIPGQS"
     gene            complement(4711..4893)
                     /locus_tag="PLR13_02275"
     CDS             complement(4711..4893)
                     /locus_tag="PLR13_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18186.1"
                     /translation="MTENKQPLPENTWKCSNCGNTVVQITPPETCPVCKQKCEFLNVT
                     CYQPDCGFTGMDKRLK"
     gene            5040..5444
                     /gene="queF"
                     /locus_tag="PLR13_02280"
     CDS             5040..5444
                     /gene="queF"
                     /locus_tag="PLR13_02280"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HQC18187.1"
                     /translation="MPKEKKKNDFAGLTLLGKAAKPVRKLETFPNHNEKRAYVVTLQT
                     EEFTCVCPKTGQPDFAKIKIQYIPDKKIVESKSLKLYLWSFRNEGVFHEHVTNIILDD
                     LVAALKPRWCKVSAEFAVRGGIGITVDAEYKK"
     gene            5468..8668
                     /gene="carB"
                     /locus_tag="PLR13_02285"
     CDS             5468..8668
                     /gene="carB"
                     /locus_tag="PLR13_02285"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQC18188.1"
                     /translation="MGKRTDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRNLGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLNIQALTEIIENEKPDAILPNLGGQTGLNLTSEL
                     YKKGVLEKHNVKVIGVQVDAIERGEDRIAFKETMNKLGIDMPKSEPATSVEEAEKIAT
                     KMGYPVVVRPAYTMGGTGGGIVYNLEELRLIASRGISASLIGQILIEESVIGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSYCTAPMLTITPELQKRLQDYSYKIVEAIQ
                     VIGGTNVQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISSILASGMTLDEI
                     PYWRDGSLEKYTPSGDYVVVKFARWDFEKFPGSEDKLGTQMRAVGEVMSIGKNYKEAL
                     QKAIRSLEKKRYGLGFVKDFNAKSLDELMEMLSEPSSERQFIMYEALRKGASVETLSA
                     KTHIKKYFIEQMKDLVGLEERILQYKKKKMPDELLTQAKKDGFADKYLAQILGKEEEA
                     IRKQRQALGVTEAWEAVPVSGVENAAYYFSTYNAPDTVPVTGNRKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGIESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEK
                     EKPEGVIVQFGGQTPLNIAAELAKAGVKIIGTSPETIDLAEDRDRFRMMMDKLGIPMP
                     KSGMASNLKQALKVAGDIGYPLMVRPSYVLGGRGMEIVHDEEMLKRYVNAAVGVTPER
                     PILIDKFLENAIEAEADAISDGTDAFVPAVMEHIELAGVHSGDSACVIPPVSIPLRHI
                     ETINDYTKKIAVEFGVIGLMNIQYAIANDVVYILEANPRASRTVPLVSKVCNVSMARL
                     ATQIMLGKKLKDLNLKNKTFRHFGVKEAVFPFNMYPEVDPVLGPEMRSTGEVLGLADS
                     FGLAYYKAQEATQQNLPSEGTVLITVEEKDKGGVLEAARAFKKIGFQILASEGTHRFL
                     KENGIEAEKILKMHEGRPNIVDAIKNGEIQLVINTPAGRLSKYDDSYIRKAAIKYKIP
                     YITTIAAAVAAVKGIAALKQGHGRAKSLQSYHAEIQ"
     gene            8920..12432
                     /locus_tag="PLR13_02290"
     CDS             8920..12432
                     /locus_tag="PLR13_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18189.1"
                     /translation="MESKDGKEDKNVNALLSLPDSTKTLGKKSKSREVNPSGLKRKVS
                     QDTRRMPQKDKPAISGASPKTAGHKNGKESFCGPEEKYALAFKYSPIGICISSLQDGR
                     YVEVNEVYSNFLGYRREELIGRTSSEINLWVDMAERDALVRELLEKGTVTDFQLRFRD
                     RNGNVHWGNVAAALVTIGQELYVLTQTQDVTDKKKIKEQFEKSEAKYRLLAENLTDVI
                     FTIDMNFNYTYASPSTYHLLGYTAEELMTMKVENLVDPETLAWFMEMLAEEMEIEKRP
                     DRDLKRSRVLEYQHIRKDGSTIWVENKITFLRDQDNNAIGIIGTVRDIAERKRAEEQV
                     CKEIDFNRSVLDSLTVPFFMFDTASMTFFRWNKTFSAVSGYSDEELGQMRPSALISEH
                     QKDFLLAKFEEFQRTGHIAFEMPVLSKNGSVTPYLLSGNLLHHDGKPFVVGMGIDITE
                     RKKAEESLRRSEEKYRTILENMEEGYFEVDLSGNFTFFNDATCRHFGYSREEMTGMKN
                     RQYMDEKNSKALYIVFNRIYETDQPVTNLEWQIIRKDGAKRDIEGFVFLLKDVAGTPI
                     GFRGIVRDITERKQVQEKLHRERQLFKSVTEQSSDIIIIADTRGRITYENPAVEKILG
                     YRVEERLGASGYDLVHPDDAQIVADAAGKLFHNIQAPVQKGEIRLHHKNGSWRTFEVT
                     ASNLVKDHVVEAAIVNMHDITARKRSEELIRQSEAKYRLLADNMKDYVWLMDLNLKIN
                     YISPSVERLMGYSLEDFKKFSLDKFLTVQSFQAAMDFFAIEMPKALGAPPDYVLERSL
                     ELEFCCKNGQTVWGECNFSLIRDDKGKPLSLLGVSRNINERKQMEDALRKSEENFRHS
                     LDDSPLGVRISTVEGKTLYANRAILDMYGYDSKEELQNTPLQERYTPETFEAFKERKK
                     KRLKGQFGPSEYEISIIRKDGEIRHLHVFRKEIFWNGQKQSQVIYEDITLRRRAENRL
                     NETLESLRQSIKITIQVLGTASEAKDPYMAGHQRRVADLARAIATEMKLPQDKIEAIR
                     MASAIHDIGKISVPAEILCKPAILSDIEFSFVKNHPRYSYDIMKEVESPWPLADIVYQ
                     HHERMDGSGYPLGLKNDEILIESRILAVADVVEAMISYRPYRPALALEMALAEIEDNA
                     GTLYDAEVVKACLRLFRDKNYQMS"
     gene            12544..13242
                     /locus_tag="PLR13_02295"
     CDS             12544..13242
                     /locus_tag="PLR13_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HQC18190.1"
                     /translation="MKKLTAIILFVLCLIPVVIFAKNASVEDQFKKSFPKNKFESIVP
                     TSIKGIYEVYSGNQLYYYMPKDEIVLYGTLVSKSGVNITRDSLLKKMAQKMEKLPLEN
                     ALKIGNGKKIIVEFMDPHCYHCRQAYQFFSQRKDVTMHVFFFPLSKESADQIKHIYCS
                     KDVLKTYDDVMKGKLDNNASLNVCADAKIDEKIKTHVQLASQVGLRGTPLFYIKGQVV
                     DGFDVALIEKLLKD"
     gene            complement(13281..15176)
                     /gene="metG"
                     /locus_tag="PLR13_02300"
     CDS             complement(13281..15176)
                     /gene="metG"
                     /locus_tag="PLR13_02300"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQC18191.1"
                     /translation="MPKSFYITTPIYYVNASPHIGHAYTTIVADVLARYHRMIGDKTF
                     FVTGTDEHGDKIAEAAQKAGLTPKQYADNISAQFRALCPQLAISNDYFIRTTDANHIE
                     TVRYILQKVYDAGDIYFGSYGGYYCFGCERFLTEKEIVDGKCPEHGREPQYIEEKNYF
                     FKMSKYQDWLIDHIKKNPEFIRPERYRNEALAFLRDPLEDLCISRPKTRLEWGITLPF
                     DEQYVTYVWFDALINYVTAVGYPEGEHFKTFWPVAEHLIAKDILKPHGIYWPTMLKAA
                     GIEPYQHLNVHGYWNSDQSKMSKSLGNIVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEDAFVGRLNSDLANDLGNLVSRAITMAIKYCGGRVPAVAGDEKNPALREAALKAVL
                     DVEASFRDMSLHKALIAVWDFINITNKYIVENEPWSLAKDPANGKKLTAVMYDLLSAL
                     HAIAVMLSPFMPQTAEKILRQIGCEKTEGLKLDDIKTDNALKTGQVLTRSDALFPRIV
                     QQKEKPEDQKAAMTDLKPEIDYETFSKVDLRAATILAAHAVPKSNKLVKLTVDIGEER
                     TVVAGIGKDYKPEDLIGKQIVIVANLKPAKLMGVESRGMLLATDTPEGLTLLTFDRAA
                     KTGGKIR"
     gene            complement(15194..15952)
                     /gene="ricT"
                     /locus_tag="PLR13_02305"
     CDS             complement(15194..15952)
                     /gene="ricT"
                     /locus_tag="PLR13_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQC18192.1"
                     /translation="MLVNIIGVKFKKEGRIYNFDAADLILHRDEQVLVETDSGLALGT
                     VVTDVKRCEPSAVPANLKKIIRKVTDDDLKIRKENDALEQEAWKFCLERIRKRELSMK
                     LVNVDCLFEKNKIIFYFTADSRVDFRELVKDLVQKFKTRIELKQIGARQETRITKGLG
                     ICGRMVCCAGILQNLERVTVKMAKEQSMSLNPEKISGLCGRLMCCLAFEYDGYAGKKK
                     AAKAAKTSGSATEAQEEKQTLDLEKMDDIDHLQS"
     gene            complement(15970..16950)
                     /gene="holB"
                     /locus_tag="PLR13_02310"
     CDS             complement(15970..16950)
                     /gene="holB"
                     /locus_tag="PLR13_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQC18193.1"
                     /translation="MSLGNIYGHEKKIDMLRKTLARRRIAHAYLFSGPSATGKKTLAV
                     EFVKAVNCISSDDRHDACDACPSCRKIQHASHPDIFFVEPEGQFIRIASIRQIQERTT
                     CRPLEAKKRAFIIDDADRMNEESANALLKMLEEPSPANMLILITTRPHAMLQTIISRC
                     QHIHFNPLAVETVARFLVSEKNMEHSRAMLLAALSGGTIGNALELNQEEVVAYRTELF
                     NTLALTDRKEPFSLIHFASFLGQKKKEIRQSLNILNTFFRDVLVFKEVGQKGMLINQD
                     NTPLIAACAARLTGKQILQNIAQIEKAGDIIEQNVNKSLTLETMAFKLNY"
     gene            complement(16984..17634)
                     /gene="tmk"
                     /locus_tag="PLR13_02315"
     CDS             complement(16984..17634)
                     /gene="tmk"
                     /locus_tag="PLR13_02315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQC18194.1"
                     /translation="MIKAKKFITFEGIEGSGKSTQIKLVAEYLQNQGVELIVTQEPSG
                     TDIGRQIAGILFHRRHNHLCAEAEMFLFCAARAQHVRDVILPALRQGTLVLCDRFSDA
                     TYAYQGFGRGLDQKFIGAVNDYSAMQLKPDMTLLFDLPVETGLERATARNNRLADPAA
                     TDRFERETLDFHRRIREGYLTILNNEPERFRLIDATRKIPVIQEEVRRHISSFIKV"
     gene            complement(17759..17938)
                     /locus_tag="PLR13_02320"
     CDS             complement(17759..17938)
                     /locus_tag="PLR13_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC18195.1"
                     /translation="MAYVITDECIACGSCESECPEGAISEGDDKYVIDPKLCNDCGTC
                     ADQCPVEAIVPGEGK"
     gene            complement(18050..18271)
                     /locus_tag="PLR13_02325"
     CDS             complement(18050..18271)
                     /locus_tag="PLR13_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQC18196.1"
                     /translation="MKEDVQKAIDTIRPSLQADGGDVELVDVSDDGTVKVRLTGACHG
                     CPMAQMTLKMGIEKVIKKLVPGVKEIVAV"
     gene            complement(18319..18915)
                     /locus_tag="PLR13_02330"
     CDS             complement(18319..18915)
                     /locus_tag="PLR13_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18197.1"
                     /translation="MIKKKSYVFVWIALILTIAGCGGLHFSQLAPEAIDFHPQSIAVF
                     PIEVWNHKEPDNRTVVQQIVAGSLIAKKLFTSVTDAEKLQKQMQNDELLKKTAEEYFS
                     KLQLLKFSDADLSRKIGDILQVDGILLLSVDEWKYFVEADKKMAQVSLTMELYDVTNG
                     KLMWKANHTIKKDYVLIKPGLPDMARDVANKMVYYMPH"
     gene            complement(18957..20684)
                     /gene="ispH"
                     /locus_tag="PLR13_02335"
     CDS             complement(18957..20684)
                     /gene="ispH"
                     /locus_tag="PLR13_02335"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQC18198.1"
                     /translation="MEIRLAKTAGFCMGVRRAVDIVLDIAQHEKNRRIYTYGPLIHNP
                     QTIELLKSRGIKPITHIDEIEDKENAILIIRAHGIAPAERKKIKETGIKVVNATCPKV
                     GYVQSIIKKHAAQTYTVVIVGDKEHPEVDGLLGYTDGRGIIISTEEEVNKIPCLEKVC
                     VVAQTTQNLDEYQKIVEKIKQLCPQAVVFNTICLSTEQRQAEVIDMAGEMDAMFIVGG
                     KNSANTKRLADLARKNQPSTFHIETPADMEKIDLGPYNKIGVSAGASTPNWILDRVID
                     KITEGRSRKLKNLGKLLTLWTFLVKTDIFSAIGAGCLCLVCMLLQTMTVRFSFILIAS
                     LFVYGMHVLNRLISRKPAGLVGSFREEYYIRHENIFFVTSLFSIILALVLAFQQSLAV
                     FFSLLIISLGGVLYNMQLLSPRWNFQSLKDVPGSKNIFMSIAWAVAAAFLPSIHETPF
                     PDAGLLVAFFFVFCIVFIRSAMSDILEIQSDKLIGKETFPVLFGKTRALNILKIISLI
                     LFAILLFASPSGWTSSLSFPLMFCLLYLWICFLFCDKRPVLSGAVKEGLLETSYIIAG
                     FCVLLWLIF"
     gene            complement(20674..21597)
                     /locus_tag="PLR13_02340"
     CDS             complement(20674..21597)
                     /locus_tag="PLR13_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HQC18199.1"
                     /translation="MKNKHLIYVLFIIVLFSTYSELLWTASGRTSPPYYNMAMGAVFT
                     CFALLHGGTYVGRKCIISLAVLTFVISFIMEYTGVKTGLIYGEYHYGDALGIKIFDTV
                     PLLIPFSWFMFMYVSTVTVDAAFGDRFAGRSAPFLFAFFDAAAMTALNVLIDPLWVTG
                     GTWTGTTVQALPSGSVFYGIPAQNYFGWLLTTIMIFIPYRMIFFRNRNRFAARDRDFF
                     LPCLIYASIITVGSIKSWMVLANTGIIFVTIMTGGVFSLAALAGFLSCRSGRSGPAEK
                     RQRQGHPDSFQEDDQISYNNKIARKTKQYGN"
     gene            complement(21644..22396)
                     /locus_tag="PLR13_02345"
     CDS             complement(21644..22396)
                     /locus_tag="PLR13_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQC18200.1"
                     /translation="MAAEKIRLDKLLVERGIAPTLEKARALILARAVKVDGAYPDKAG
                     TLFVPHTEIHIKGEDNPYVSRGGLKLAGALKEFDMNVEGLVAFDVGASTGGFTDCLLQ
                     AGARKVYALDVAYGQLAWKLREDKRVVVIERTNIRYYDGADLDEKIDLAVIDASFISL
                     KTVIPPVLKFLEEGTRILALIKPQFEASKKDVGEGGVVRDPTIHDRVVQNISDFCISL
                     DLDVEGTCASPISGPAGNKEFFVLTRKKPSQT"
     gene            complement(22439..24361)
                     /gene="dxs"
                     /locus_tag="PLR13_02350"
     CDS             complement(22439..24361)
                     /gene="dxs"
                     /locus_tag="PLR13_02350"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQC18201.1"
                     /translation="MVKQPQTVHYSVLKDVNFPADIRGLTLAQLNTLAQEIRDLITLT
                     VASCGGHLAASLGTVELTLALHYAFNTAQDKIIWDVGHQSYAHKIITGRKEDFKNLRK
                     QNGISGFPRREESPYDVFNVGHSGTSISAAAGMTEAACLKGASYKIIAVIGDGSMTSG
                     MAFEGLNWVGDRKKNMIIVLNDNEMSISENVGAMSSYLNRVMTGHKITTLKADIKRFL
                     KSIPGVGGDMIKFAQKIEEILKTLMVPGALFEEMGFTYVGPLEGHRLDHLIKTFENIR
                     EMTGPVLVHVITKKGKGYSFAEQNPPSYHGVAPFDVQTGQVIASSSGIPTYTNVFGRT
                     LVKLARTDSRIVAITAAMREGTGLNLFSQEFPNRFYDVGIAEQHAVTFAAGLATEGFI
                     PVVAIYSTFLQRAYDQIVHDVCLQKLPIIFAVDRGGLVGEDGATHQGLLDYSYLRSIP
                     HIVVMAPKDENELQHMLKTAVDCGQPVSLRYPRGSGAGRPLDEVLTALDIGKGEVLRG
                     GTDLAVMAIGSTVLPALAAANRLAEEGRDISVINARFVKPLDEELILKTAAAVGTIIT
                     VEENTLQGGFGSAVLELLAEKSITGIRVKRLGIPDEFVRHATQAQQRRRYGIDEEGIM
                     AAINEILTSESFRRPS"
     gene            complement(24380..25282)
                     /locus_tag="PLR13_02355"
     CDS             complement(24380..25282)
                     /locus_tag="PLR13_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQC18202.1"
                     /translation="MMQDDDLFLKAYLEDRQNIVEEALQRYLPEDGFPSDIYKAVHYS
                     VFNGGKRIRPILCLAAAEAVGGDLVPAIPVACALELIHSYSLIHDDLPSMDNDDFRRG
                     KPTCHKVFGENMAILAGDALLTEAFVLLSRAEKVMLSAERRLAIIQEIANAAGINGMV
                     GGQALDVISEKTGADETILQDIHRRKTGALIVAAVKSGAVMFNAGKDKIQALTEYGMH
                     VGLAFQIADDILNVEGDSRLMGKGTGSDAARNKMTYPSLMGLQQAKEKLNQHVEAAVT
                     SLADFDERAMPLIVIARYIRDRKS"
     gene            complement(25269..25508)
                     /locus_tag="PLR13_02360"
     CDS             complement(25269..25508)
                     /locus_tag="PLR13_02360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQC18203.1"
                     /translation="MMAKEKFEDALEKLEDIVKKMEAGDMPLDETLKSFEEGIKLIRF
                     CSAKLEETERRVELLLEKENTLQVKRFQGDDHDAG"
     gene            complement(25631..26962)
                     /gene="xseA"
                     /locus_tag="PLR13_02365"
     CDS             complement(25631..26962)
                     /gene="xseA"
                     /locus_tag="PLR13_02365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQC18204.1"
                     /translation="MEQILTVSELNNTIKSLLEESFDSLLVEGEVADLRRPQSGHTYF
                     TLKDDKSQIRAVFFRPFGRTGGRVNFELEEGLKILCRARLTLYPPRGEYQLVVESVEP
                     LGVGALQKAFEQLKAKLSAEGLFDKQYKKALPFLPERIGVITSPTGAVIRDILNVSRR
                     RFPATDILIAPVRVQGNDAANDMIQALRRFHAFGQVDVIILARGGGSLEDLASFNDER
                     LAREIFRSDIPVVSAVGHETDFTICDFAADLRAPTPSAAAELVLPDRTELIFKVNMLA
                     RRMTAGCLRHLKAEKEHLEGLQERFKDPRLAVVTQKIRIDDLKESLSAAFGHRRQILL
                     HKLNHLELRLKHQSPEQQINEKQIVLKNLRKELHSSLSCVLTALKERFQKDAAVLESL
                     SPLAVLERGYSLTRTVPEGTLIRQTRDLHEGDEVNVLLAKGNFNAKIQKIF"
     gene            27133..28470
                     /locus_tag="PLR13_02370"
     CDS             27133..28470
                     /locus_tag="PLR13_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQC18205.1"
                     /translation="MERKMFWKKIKNAVCPVKHILRVPVGLFIVALVCADAGAAQNND
                     RLIQAAEKGLLQDVQTALAQGADTNAKSVKKYYNIFGKEITPLIAASENGHAEIVKLL
                     LQKGADPNFQRLPERTTALMLAAENGHADVMKILLEGKADINAINNTGITALMLASKN
                     GHPDAVGILMQGKVDVNTRNNDGVTALMLAAQYGHHDIVQRLVQHQADVKIKNNDGVT
                     ALMLASKNGHAKVVERLLETDAGVNVQRASDNATALMMAAENNHREIVGLLLNKGADV
                     NLKTKFIGATALILTAAKGHADVARLLIDKGADMNAKNKDGITVLMVASLNGHRAVVQ
                     LLLDKGVPVNGKSNENITALLMASERGHKDIIQILLDKGADVNAKDSDGLPPVLLAAQ
                     KGHKDVVTLLLDKGADGNVRAKIKDTEYTLLTAAEINKWTDIVERLKKSGVKE"
     gene            complement(28721..30178)
                     /locus_tag="PLR13_02375"
     CDS             complement(28721..30178)
                     /locus_tag="PLR13_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18206.1"
                     /translation="MMVSSRQKKRIVLVHPRGFNWFPGKRDITDIANRMVPQGVLSIA
                     AYLKQKGHEVFVYDCLGPGAPLDLQKQAGAILAYQPQMVGFSATTSSFPDAADLARLI
                     KNHSSAIATVCGGVHVSALEGKLLNAYPAFDFLIAGEGEETIAELADEIPAADIRGLI
                     WRNQDEITVNDVRPKISDLDSLPFPAYEQLKGFPRDYHLPLFSYIHTPGATMITSRGC
                     LYQCSYCDRSVFKKGFRYNSAGYIYDHMKYLRETFGVRHINIYDDLFTANRPRIVKLC
                     EKLSRRPLGLHFNCAVRIGYTDDDLLDMLKDAGCLMVSLGIESADPAMLARHKSGINL
                     DDVRDTVKRIQKAGLRAKGLFMMGLPGETEASVKRTSDFILSLDLDDMNMAKFTPFPG
                     APLWTAIREEGTFHEDWRLMNCLNFVFVPRGIASKERLDQLYNEYVKRFYSDKKWRKK
                     FRNRIWQHRNSLLYLIRHLPSFWSAKNQFEPQKGK"
     gene            complement(30175..31164)
                     /locus_tag="PLR13_02380"
     CDS             complement(30175..31164)
                     /locus_tag="PLR13_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18207.1"
                     /translation="MTETTTKHTAPASPIPVIAASYIGPPLMPNRDALWEENELPLNA
                     DALVNEVMGQPMRRAGRFIKMVSCGGLACLQQVSPDYLKGKKTGIFLATGLGNVDEIL
                     PFITQVFKHGGGFPSPNQFANSVSNAAAFVMARICEVTGTVLTVSQEELSFESALWLA
                     QTYLEHGRIDIALVGGCDIFTPTAEEYRERVNVSCGNARRLPMGESSSWLLLGKNEEQ
                     NIGHILAVDFYDTVGAPDQRFDKQTILSDLCDARGKGRIVLPGFRIRQTDLNGWKDGG
                     WETEDYLPCCGIHSTASAFGIARTLREKTNSVFVHYNCSASGRHAFVSGSTKS"
     gene            complement(31207..32403)
                     /locus_tag="PLR13_02385"
     CDS             complement(31207..32403)
                     /locus_tag="PLR13_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQC18208.1"
                     /translation="MNSGDIYVRGWGAVTTLGWNATDAARNLIEAQAPPAKPGRSSHL
                     INRDFNVFEVSHPGNPLEKAMAMLEGAIDEAFERAGLSPDEIAESSVLIGTTGGLFIR
                     NEHEFIEAVRKNPDGETPPISCRNRGAGEVADFITKKYGIRGMALTFSIACVSSSHAL
                     VVAADLLQSRKIRRAIVIGFEPLLNMTLHGFSSLLLFDYERCRPFCATRAGMQIGEAA
                     SAVILDSGQNLSSRYTFSGGYLYNDIKSMTSAGIDGVTVSEVVRHTLDSARLSADDII
                     AIKAHGTGTKDNDLSEGRGLVRLFGEKLPPVVSLKGAIGHTLGAAGTAETALWLSCLD
                     EGIIPRSFGFSEMDPEIGFTPSQKNIDARDGAYLFTFFGFGGTCTSYLIIRNSTENSS
                     ARAGSS"
     gene            complement(32400..32711)
                     /locus_tag="PLR13_02390"
     CDS             complement(32400..32711)
                     /locus_tag="PLR13_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18209.1"
                     /translation="MDVTQKLKDLNVSSREELIYKLKELVIRACEITDVRSEDVPTDV
                     PFIGGPGPLLLDSLDAMEIAMEIRFQFGVELKNASTAAKAMQNFDTLADFIIEAPRVK
                     K"
     gene            complement(32702..33823)
                     /locus_tag="PLR13_02395"
     CDS             complement(32702..33823)
                     /locus_tag="PLR13_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18210.1"
                     /translation="MTFRKLTALIRKEMIILLRERQTIPLLFIMPMALIFFLSLALQG
                     VYMDKLTGRQILLIIENESHQPTANLLEQRISANKMIQRVQRPPGFDNDKVFETSRAH
                     AVIFIPKGFDEGLKPVEMEFDPVLDAGYKSAVRVLVTGLTVEVVMGIDDIEGMSADFI
                     VEKTKTNRTLPGPLQNSVPGWSIFAMFFIAIPMSIGFLREKNDGTLQRIFTYPVSPAI
                     VILGKLIPYYLINLMQFALMLIVGIYILPLIIAFPFTLGEHPWHVIPVTLVVAAASTG
                     FGVMVAALARAPEQSSALAATGAVLMAVFGGIMVPHAVMPLIMKKLAMLSPMYWAHQA
                     YLDIFSRDAGFGSIWPKLAVLTVFALVCFYIAGRKVKWM"
     gene            complement(33839..34585)
                     /locus_tag="PLR13_02400"
     CDS             complement(33839..34585)
                     /locus_tag="PLR13_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18211.1"
                     /translation="MKALDIAQVTFSYNPKEGKIKTAGAEYALRDFSMSVDSGQMHAL
                     LGPNGAGKTTLMRLITGQLRGFQGSISIYGTRNDHAQSLSSVGYAPQPISVYAGLTAM
                     ENMRVFGVMAGLSRELLQERSRAVLEETGLLPYAQKRASTYSGGMLRRLNLAVALLHA
                     PRLLLLDEPTVGVDPQSRNHIYETLKKMNASGIAIVLCTHIMDEAQRMCSKVTMLDRG
                     AVIFSGAMNEIDHLETFFLAQTGHRLRDED"
     gene            complement(34582..35619)
                     /locus_tag="PLR13_02405"
     CDS             complement(34582..35619)
                     /locus_tag="PLR13_02405"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HQC18212.1"
                     /translation="MTHTELTSGGKPFVHHHAAHCETGVTAALFRDKGLEISEPMIFG
                     IGSGIFFGHLPFVKFVGLPISTYRAQPGAVFRKAAKRLDGKFVTKKYTNAKKGMDELL
                     RVLRSGQIVGLTTNIYWLSYFPDRFRFQFNGHNIVILHEIENGFRVSDPVLDKPVDCR
                     TEDLIRARFARGPLEPHGFMYYPVSVNLHPDLRGACIKGMLDTCNMMLRIPVPIFGIR
                     GIRFLAKRIIKFEKTQGFEEACRQLGMIVRMQEEVGTGGAGFRYMYAAFLQEAADLFG
                     SAELNDLSQDMTAIGDTWREFAVTAARIIKKRGKQDETFAKAGGLMLICAGREENLFK
                     NLHEIAGKLKS"
     gene            complement(35847..36260)
                     /locus_tag="PLR13_02410"
     CDS             complement(35847..36260)
                     /locus_tag="PLR13_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18213.1"
                     /translation="MKVNEVPQDNTVEYYEGVKRACYAVDEKGRYVIVSSNGWEAEEL
                     VNGLAVKELAVNLEKTRKAVLAGEKSPLAYHMERRQMIPDILGKTAGIAVFRVKRHLR
                     PEIFSRLKNPVLERYAKALAVTMEELKTVPEESRE"
     gene            complement(36527..37420)
                     /locus_tag="PLR13_02415"
     CDS             complement(36527..37420)
                     /locus_tag="PLR13_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18214.1"
                     /translation="MKKLPYKLLLFFTHRLGLWFFRIISWFIATGYFFLFPVRVANSF
                     EFYRTLFPGKGFFYHLWCAWQQYHKFTDVYIHRFIRFDENDIEYIKEGWEHLDAAVQN
                     KTGAILLMSHIGNWELAAQTLNKRGLPIMLYLGAKHKEQIERIQKEAMAKSGIKIVAT
                     SEDEKSPFALIDGINFLREGGIVSMAGDRLWGDQSHIAVNFLGHEVRLPDTPHLFALM
                     TGAPLMTFFVYQEAVGKYHIRISPGRKVTAAARADRKKAVQNSVQAYADDLAEFARQH
                     PFEWHHFEPFLEKSHSASLRE"
     gene            complement(37520..38590)
                     /locus_tag="PLR13_02420"
     CDS             complement(37520..38590)
                     /locus_tag="PLR13_02420"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HQC18215.1"
                     /translation="MSVVFSGCSGSSGSDSESSPRIATTSLPSCVIASAYSQQISVTG
                     GNGTKMFSITKGSLPSGITLSSSGRIAGTTWAAPGVFQFTVTVEDAAGSSTQDLSLAV
                     VADNIAGPHPYETIRNNPSYYYVSQEAGLCAATCFYMTMKYYGDHLKNIKTGIDDDNC
                     PAEITDRIAYPTELSATSQIATYIQYMDNSFNNLAGIHLTSLARAAENLLDESGSRAL
                     YSEVMIENYSNDLTGTEDSISEQKRNIFLNRMVPFLNSGSPVLVHLWRSPVLGIPSSG
                     HYVLVIGYDDAEKAVYFMDPNDQNHKGNQCDCVAVDSSNSDICFIQKVAFDHFIEEYW
                     YKSDDPLVLNARWDGNWIGFHH"
     gene            complement(38806..39576)
                     /locus_tag="PLR13_02425"
     CDS             complement(38806..39576)
                     /locus_tag="PLR13_02425"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQC18216.1"
                     /translation="MNIIQTIILAAIEGITEFLPVSSTGHMILASSLMNIHDNAFVKS
                     FEIAIQIGAIGAIIMLYYKRFLKGIKIYIKLGIAFIPTAVIGFLAYKTIKMHLFNPLI
                     VSTALIVGGVILILVDKRVVNSKTQYMELEDISYKHAILIGLAQCVSMIPGVSRAAAT
                     IIGGVFNGLNKKQATEFSFLLAVPTMCAATGYDLIKTDAAFSSYEQTLLVIGFAVAFI
                     FAYLAVKIFLKIVENYGFKYFGYYRILIGLVFIIYAVR"
     gene            complement(39748..42807)
                     /locus_tag="PLR13_02430"
     CDS             complement(39748..42807)
                     /locus_tag="PLR13_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQC18217.1"
                     /translation="MPLIRKYQVNWSIIFVVALIVTGLSVWESRNLKIDTDILESMPH
                     KDPVLADARLIIKHLPVQDKVFINLEQTSPDRNKLIDTAAAVTDQLNKSGLFIRVGIG
                     DDAENFPELIAHVNDNLPALLSADDLEQKIAPLLTPDNIREAMAQNRQALGQLEGIGR
                     GDMMTKDPLGFSSVILKQMSALLPANKALFYQGQLISEDGTNALIVARIKGSGTDTAV
                     AEKIEKLLEDCRRDLAAKGHPYKLTATGAYRVALDNETTAKSDMQMAVTFTTLGIAFL
                     LILAFPRPLIGLLALLPSSVGAIAALFVCSFLFKSMSIIAVGFGSAIMAFTVDLGIAY
                     LLFLDQPHATCGKDVAREVWSAEFLAVLTTVGSFLLLLISDFKILAQIGVFSALGVAF
                     ALLFVHLVFPRIFPAMPPARKPGNPLLSNAVQKFAAPAKWKLIAAIIFGLAMLFFAKP
                     VFNVDMNSMNSMSEATMNADKKMQEVWGNLSGRCYVFLQASDMTQLREKNDQLAGWLA
                     GDIRDEKLSPPFLPTVLFPSPETAQHNYAAWSAFWNKDRVAELKRNLSAAGKENDFAP
                     DAFEPFWKIINRTTIGSFDIPEKYFDMLGIAKSSAGYTQLSLLASGKNYNAEDFFERL
                     SRSGLAKIFDADLFNKRLGDFLKDLFLEIAIIISIGLVLITFLYFLDWQLTLAAMAPI
                     VFALFATLGTMKIIGHPLDIPGIMLWIIIMGMGDDYSVYYICHYQRNVDEKSPAMHTI
                     KLAIFLAALTTLIGFGVLALADHALLRSIGIVSFLGIIYSLIGVFFILPSLMDKIFAP
                     IAYPADKVVIGSRDHLRRTVLRYRHLPGYPRVFARMKIMMDPMFRELHKYVKNPRRII
                     DIGCGFGVPATWLLEIYPDARVFGIEPDVERVLIASRVIGSRGRVEAGRAPDLPHVEG
                     EADHVLMLDMLHLISDDEVKIVFQRIYDKLTADGTLLIRTTIPSDKPNPWKRWIETSR
                     LKMTGMPNRFRSETEIADFMKQAGFAVQVFASDTRSIEEKWFAGKKY"
     gene            complement(43076..43678)
                     /locus_tag="PLR13_02435"
     CDS             complement(43076..43678)
                     /locus_tag="PLR13_02435"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18218.1"
                     /translation="MKNPDLDTGIEDMHIYRPQISKVEYQDVNRIFEKETLQHEIGFV
                     KLKNGGYLVAMETGRPDVKRKMVRWWFWRHVQEKEHQIVYPGEHLSIRYSSRNKDYFN
                     EPYGDFEKNTVYPVGRAGKKVIRLFIQFVHPRTFGISESNIKRTENGFLLCGFVGVMK
                     GMRKHTKMLHYFKSNGDGIRPISRFGLGNGLPRVLKSIAQ"
     gene            complement(43848..44567)
                     /locus_tag="PLR13_02440"
     CDS             complement(43848..44567)
                     /locus_tag="PLR13_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC18219.1"
                     /translation="MTRSKDYNKNRFAFVIPVYNHAGTVAQVAKDAQKLGYPVVVVDD
                     GSTDDTYNQIKEIKDIQLLQHEQNQGKGAAILTGFTAAAKMADWAIIVDADGQHYPED
                     AKKLIKAIPKNTRPIVVGAREGMAGKHVPWTSSFGKKFSNFWVRASGGPAISDSQSGF
                     RIYPLPEALNLKTKARRFQFEVEILVHAERNGIPVIEAPIRVRYNPEGGRISHFRPFV
                     DFCRNSSTFTRLIFTRIFPPR"
     gene            complement(44583..46304)
                     /locus_tag="PLR13_02445"
     CDS             complement(44583..46304)
                     /locus_tag="PLR13_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQC18220.1"
                     /translation="MNEVLKQSFTAGVERLMARSDLLDSINVFPVADGDTGRNLSLSL
                     MPLRNTDLPQATMIHRLLLSARGNSGNIASQFFSAFCAVENIADLAGRAREGKIKAWQ
                     ALVNPRRGTMLNVFDALADVLSEQFTPDKENIENIIRSMENAVHQTYEQLPKLQEAGV
                     VDAGALGIYIFFEGFFYTLTGLSEDGRPLTEIFHDRLHISSSFQENIESGYCVDFVVK
                     APRYSPDEIKMITSVQEEVIVIPEGEFYKVHLHTQDKEQVRSQITGLGSLVTWEDDDL
                     SCQTADFRHLNAEKTLHIITDAAGSLTTQDAKIHGFTLLNSYLTIGDQCLPETFFHPE
                     DLYRAMKKDIRVSTSQASVYERHQFYQSALERFDKVLYLCVGSVFTGNYQAAVQWKKD
                     HDKEDRFSILDTGAASGRLGTIALSTARYLAESRDVTNTIIFARKAIRLCEEYLFLDK
                     LHYLAAGGRLSKTSAFFGDKFNVKPVISPLPDGAKKVGTAKNQEDQITFALAKLDATI
                     KDDSRALIMLEYSNNRRFVDGTVRKNIKELYPDAEIILQPLSLTSGAHMGPGTWGVAF
                     LPQINIR"
     gene            complement(46306..46722)
                     /locus_tag="PLR13_02450"
     CDS             complement(46306..46722)
                     /locus_tag="PLR13_02450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQC18221.1"
                     /translation="MKEKQNSIDVKIRVPFFDLDPMQIVWHANYLNYFEIARAALFES
                     RGIDLYSFYEKHQLIFPIIKTSTKHVLPLFHRDEIICKATLVEARIKLIVDFEIRKAE
                     DGKIVARGRTEQVTVKVPEREMLFRIPEEISRAFGF"
     gene            complement(46719..48272)
                     /locus_tag="PLR13_02455"
     CDS             complement(46719..48272)
                     /locus_tag="PLR13_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HQC18222.1"
                     /translation="MKKNVNRSAVIIDGGKIRFNDIIAVARYGVAVKISKDATFVRKM
                     KKTRDMLMQSMRTGIPIYGVTTGYGKSCGKRMPFDIALKNGVNILRFHGCGTGEPIGV
                     EETRAAMVCRMICLARGYSGVSIALLQQIADFLNHGITPIVPCEGSVGASGDLTPMSY
                     IAATLMGERDVIYRGTVMSAARAMKKAGLKPYTYLPKEPLAMLNGTTTMTGIAALTIE
                     RAYRILSAAIYATALSIHALKGNARHYHPVISEAKPFPGQAFVAAQITRLLKAKISTR
                     HLEDDSLDALQDPYSLRCTPQVAGVLYDGLEWIRTWVEIEANSSNDNPIFDPATGEIL
                     MGGNFYGGHIAFAMDGLKAALASVADMCDRQIMLLVSPNLNRGLPGDLVRKPPHEKGL
                     RHGFKAMSISSSALTAEALKLTMPAASFSRSTESHNQDKVSMGTIAARDAERICTLTE
                     RVVAIHLLAAAQACDIRDNTNARPLLSKTMGKIRSVSAPVIEDRAMDKDIENICTAIR
                     YSDFFKIKP"
     gene            complement(48276..49535)
                     /locus_tag="PLR13_02460"
     CDS             complement(48276..49535)
                     /locus_tag="PLR13_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQC18223.1"
                     /translation="MKFKLIYPKWEKLDRQTRFTLPPHGPVVFAATLPDDVDVEFIDE
                     NIKDIDFDEPADFVGISVMLTAQIKRAWDIADRFREKGMKVIFGGIATMLHAEETQQH
                     ADSVFLGEAEGRMEQVFDDFKNGNLQKVYNYMQNFPPIESVGTARRSILDRDMYNYRG
                     VQMVDLVHASRGCRFNCYPCAVRFLGGRQFRPRPFDKVVEEMASIENNRLFIVDNSLA
                     QDKNWETQLFKEMIPLKKKWCCHPIEKDDAVLDLAAQAGAWFVYQTVFDTSDFIRDRI
                     KRYHDFGIGVEGSILLGLDYHTEDYIKKLVDFLLDIELDMAEFTILTPFPHTRTFDDL
                     HNAGRIFSYDWSDYTCGKVVFQPKVLTPGKLQELYYYAWDKFYEEEPANYKMFKLLKK
                     VMDKEKADGTYKPRVRPKVIRDKKGVS"
     gene            complement(49644..>50889)
                     /locus_tag="PLR13_02465"
     CDS             complement(49644..>50889)
                     /locus_tag="PLR13_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQC18224.1"
                     /translation="VMNADRPQDDARRVVVSGIGMVTPLGIGKEEFGRKLFAGHTAIK
                     DIRSFDTGAFSSHLGAEVTEFKPRDFIAVKNMRRMDKISLMTVASARMALEDAGLQIT
                     AENRDRVGIILGTAFGATDITVQFLHTLLQEGPASVNPILVPNTVMNAPAGHTSIELG
                     FRGINTTITHFAVSAENAIAYAAAEIRRGTADFIFAGGSDILSEIAYRSLIGFGVLSP
                     QDKGNEICRPFDQERNGCIAGEGCGMILLESLQSAKQRGCNYYCEISGAGMGASPTRP
                     NEWPRDTEGIKRTFARAVKNAGITISDLQAIFAAANGDKILDAAEAEAYAEIFDETKR
                     PCITSIKGATGESFSSGGIKACAAALSMANQSLPPTAGLAKPVRALEFVIQEKKEADI
                     HHTALAGISFGGTYCYLIFSKL"
     assembly_gap    50890..50989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(51030..52277)
                     /locus_tag="PLR13_02470"
     CDS             complement(51030..52277)
                     /locus_tag="PLR13_02470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQC18225.1"
                     /translation="MQKRIVITGMGTLNAIAHNIPDFSPALKNGHCGIREIDIFDTSG
                     FSARTGGQIKNFAARNYIPQNFSMKRMSRADLLSFAATLEALHDAGLYPFPEELQKDT
                     GVSIGGGAGGLLEAESFYANFLKSNGRRARFSRLASLFCASSADRIASAFGLAGPKAT
                     FMTACSGGATAIGYARDLIAGGQASVVIAGGVEPMCRLTYAAFNALKSVAAATCRPFD
                     KNRDGLSLGEAAAIVILEELDSALARGARIHGEILGYGITCDSYHMTAPDEQASGAVR
                     SMEAALRDSGLSPDDIDYINAHGTATPVNDAMETKAIKNVFGKRAYTIPVSSTKSMHA
                     HTLGAAGALEAIVSILALRDGFVPPTINHQERDPQCDLDYVTSGARSANLKTALSNSF
                     AFGGNNTTLIFGKYTERSARHER"
     gene            52545..53072
                     /locus_tag="PLR13_02475"
     CDS             52545..53072
                     /locus_tag="PLR13_02475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC18226.1"
                     /translation="MAKKNPETKYEEKVFVKERIIAKRLKNSKKEAADQEIKAARKAE
                     RLDEENEVLITILSEIENLPKEDILYNYSEDISMTGTRIQGNCLLPVDTFLKIDLVLK
                     NLKQTVTVFGKVKWSKPAEDVKSYEAGVEFVDTPEESIKKLGDYILSINQYKNLNPVG
                     VPYWIFAKFNKPSSK"
     gene            53345..53620
                     /locus_tag="PLR13_02480"
     CDS             53345..53620
                     /locus_tag="PLR13_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18227.1"
                     /translation="MKKTLRTIAVAAAVVIFTATFGFAEYAATGAADFPYFQLGCLIV
                     GGLILMTLKRKYEKMYVAEMVGAFALYTLLMALFTNPVIDAVKTMVM"
     gene            53851..54345
                     /locus_tag="PLR13_02485"
     CDS             53851..54345
                     /locus_tag="PLR13_02485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQC18228.1"
                     /translation="MNFLALDFETANYYQDSACALGLVRVEDNKIIHRTSFLIRPPYR
                     YFVFTHIHGITWGDVEDQPTFDQCWKSIKPFFEGIDFAVAHNAGFDKGVLRACCRRYA
                     ITPPDLDFQCTMKLSRKVFNLYPTNLPAVCRNFNIDLQHHDALSDTLACAKIMIEIIR
                     KTNT"
     gene            54640..55881
                     /locus_tag="PLR13_02490"
     CDS             54640..55881
                     /locus_tag="PLR13_02490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQC18229.1"
                     /translation="MTSKQNIRVVITGVGLTAPNANHLNDFRRALLNQKSGISEMEVR
                     YMGKVAAGNCNFDEYLYQKKKMRRRGTRAGSISIYCAHEALKDSGLSLESLVKDRTGI
                     YLGITEHGNVETENEIYELHQNNLDAGLWSHHHNPRTVANTPAGEVSLNTGITGPAYT
                     IGAACAAGNIGVIHGMQMLQLGEVDLALAGGVSESTGTFGIFAGFKSQGALGSHEDPT
                     KASRPLDINRNGIVVSEGGAVFVLERLEDALQRGAKIYGEIAGYHVNSDAVDFVLPDT
                     QRQIQCINTAMAKANISPRDIDIVNLHATGTVSGDENECEAVRAVFGQSDNTYVNCTK
                     GFIGHAMGAAGALELAGNLPSFTDHKIHSCKYIENVDPKCNVKNLVNGDPVKKDVNFI
                     LSNSFGMLGINSVLIVKKYKQ"
     gene            55902..56159
                     /locus_tag="PLR13_02495"
     CDS             55902..56159
                     /locus_tag="PLR13_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQC18230.1"
                     /translation="MDYQTIRKKIIDIIAEIAIDEDLSNLDDNIRLREQLDMDSMDFL
                     DVVMELKKRYRIEVPTEDYMKLATLKSCIEYLGPKFAVSPA"
     gene            56159..57544
                     /locus_tag="PLR13_02500"
     CDS             56159..57544
                     /locus_tag="PLR13_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQC18231.1"
                     /translation="MTQYDTVIIGAGMSGLAAGIRLAMFDQKVCILERHKIPGGLNSY
                     YDRQGRKLDVGLHAMTNFARQGERGRPLTKLLKQLRIPYEQLELSQQNFSRIVFPAHD
                     LAFANDPQLLIQDIGKHFPHQKDPFARLLDVVINFDDVNLSNKPQSAKEKVREIISDE
                     YLLEMLFCPLLIYGSAWENDMDFSQFVIMFKSIYLEGFSRPRNGVRTIISLLIEKYKQ
                     AGGELRLGTGVKKIDVENESVTGVTLDSGESIRTDKIMSSIGLPETMNIVSEKSDRPA
                     VGNMSFAENLLFFDKKPVEAGIKETIIFYNDNDTYDYKKSRDLCDYRSAVICLPNNFG
                     ADDYDEGLIRITFIANFDQWNALDRKTYLQKKKEVCNAALSLVAKVIPQFQAKLLYHD
                     VFTPKTINRFTGHFKGAVYGTTEKVRDGRTGIKNLFICGTDQGFLGIVGAMLSGISMA
                     NLHCLMNGEKA"
     gene            57541..58572
                     /locus_tag="PLR13_02505"
     CDS             57541..58572
                     /locus_tag="PLR13_02505"
                     /inference="COORDINATES: protein motif:HMM:NF006720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HQC18232.1"
                     /translation="MRFKNAVISSFAYHLPDQVMTSEEIEERLAPLYERLKLPAGRVE
                     LMTGIKERRFWPPGTMPSDLSTQAARNLFEKNIVQPDQIDLLIHASVCRDFLEPSTSS
                     IVHHNLELQTDIMFFDLSNACLGVMSSFVVAANMIDNGFIKHALIVSGENGGPLLFQT
                     IDRLLNDKNITRKSIKKYIANLTIGSAAVAYTVSHKDHVHEGHCLLGGASLVDSSANV
                     LCRGGGDTQSLMMETDSEELMKSGVALAEKTWAETKRELGWTHDDVNCFVGHQVGVAH
                     ETLLKQILKLEHCPTFTTYQFLGNTGASALPVTLAIYDEEKKIPAGSQVGLLGIGSGL
                     NSIMLGVKW"
     gene            58566..59426
                     /locus_tag="PLR13_02510"
     CDS             58566..59426
                     /locus_tag="PLR13_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC18233.1"
                     /translation="MVRPDWLNKLYPFTSHFYQLKDNISLHYLDEGQGDPMLMLHGNP
                     TWSFYYRKLVAAFSISNRVIVPDHIGCGLSDKPQQYAYTLHQHIRNLDKLVSYLELKN
                     VVLVVHDWGGPIGFGLLKRRPELVKKIVILNSAAYTSKVIAFQINMCRIPILAEQVIR
                     RLNGFALAATYMAVAKKMSPEIRKGYLYPYDNYENRIAIAQFVADIPMTPGHRSYQTL
                     KSVELSLAQHRCPVLILWGKRDFCFHDYFLNRWQEIYPHATVKTFEDAGHYVLEDAGD
                     DIIAELGKFV"
     gene            59459..61084
                     /locus_tag="PLR13_02515"
     CDS             59459..61084
                     /locus_tag="PLR13_02515"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HQC18234.1"
                     /translation="MNIADRFEEIAKIHHDKVAVKFPVRTGKKYRYKAITFGELETLA
                     GKYANGLSEIGFRRGIKTLLFVRPSLKFPALVFALFKIGVIPVFIDPGMGRKNLLAAI
                     EEVAPEGLIAEPEVHLLRILFNNSFKSVRFKVTTKGPKIGNCFPLSMLKRARAGFQSA
                     QLEPDETAAILFTSGGTGCPKGVVYTHKIYYEQTRLLQELFSLDENEIDFPCFPLFSL
                     FTLCMGMTSCIPDMNPSQPAKADPKQLVQNIKDNKVTFMAGSPAIWEALGSYCIKNNI
                     TLPSVKYLVMFGAPVRNELHKRFSRILPNGTTYTPYGATESLPVANISGQMVLEQTAA
                     LTDEGKGTCVGEPAPGVKVAIAAINDNIIPHIRQARILGLHQIGEIIVKGDMVTAGYY
                     QSPQETALAKIADGASFWHRMGDLGYIDDAGKLWFCGRKSHRVETPSGLLCSVMCEAI
                     FNTHNGVRRSALVGIGEKGRQSAAIVIERNSAGRKLDRKRLEKELLDLGGKNPLTAAI
                     TSVHFCDSFPVDVRHNIKIDRLKLADDVARGRI"
     gene            61081..62076
                     /locus_tag="PLR13_02520"
     CDS             61081..62076
                     /locus_tag="PLR13_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQC18235.1"
                     /translation="MNIIVTGAGGFLGRNLANGLLSKGHKVRNFSRRNHLPLQAMGVE
                     TICGDLQNASEVMAAFQGIDAVFHVASRVGIWGDYDEYYRTNVVGTGNVIKACRANGI
                     TKLIYTSTPSVVFDGNDILRADETKPYALKFYNDYSRTKTLAEQKVLQANDETLSTVA
                     LRPHQIIGPDDPHLVPRLVNAAKRGRLKIVGTGKNLVDVTYIQNAVEAHLLAFEKLSP
                     QSSVAGQAYFIGQERPVVLWDFINKILTRYNLSPITKRVPEKVALNMGALFEAWYRFF
                     KINGEPPMTRFVALQFSRSHYFSHEKAKNDFGYVPKISIEQALDLTTERGMNAFK"
     gene            62063..63481
                     /locus_tag="PLR13_02525"
     CDS             62063..63481
                     /locus_tag="PLR13_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC18236.1"
                     /translation="MLLNKNDKITQHYDVIIIGGGLGGLTAANKLAKNGRKVLILESH
                     DKLGGFATWFYRKKHIFDVSLHGFPVGMIKTCRKYWNKAIAEKIVQLKRMRFINPMYR
                     LETDYTVEDFTEKLIACFNVSKEQVDNFFSLVMNMNYYDNKAMTNGELLESFFPGRKD
                     VHRFLIEPIVYANGSTLEDPAITYGIVFSNFMSKGVYTFQGGTDLVIGMMQEELMKNN
                     VDIRLKSNVGKIVVENNVCRGVMLGGEFVSSTSVLSNGNLIGTIFNLTGEDNFKPEFI
                     DKVKKVRLNTSSCQVYMGIRQGEAIEDIGDLIFYSEAKEFETDELLSMNTTSRTFSFY
                     YPRLRPQLENAGYTIVASTNARYEDWAELSKEDYKQKKRELIEATLVSLEKIIPGIRQ
                     KIDYVEAATPLTIKRYTHHEGGSSFGTKHEGLDISMKLHHEISGLFHTGSVGIIMSGW
                     LGAANYGVIQSYEVEGYLDKLN"
     gene            63490..64230
                     /gene="fabG"
                     /locus_tag="PLR13_02530"
     CDS             63490..64230
                     /gene="fabG"
                     /locus_tag="PLR13_02530"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQC18237.1"
                     /translation="MFDFSNQRVIVTGGTRGIGAGIAEAFLQAGATVIAVYSANDAAA
                     NEFKNKNEQFSDRLDIQKQDISSSQDVNNLFQYLDEKYPSLEVLVNNSGIRQDAIAAL
                     MTDEQWNKVLEVNLTGTFYMSREIIPRFMSGRFGRIINISSLGGDIGFQGQANYAASK
                     AGQLAIAKSLSKEVARKGITVNCVSPGFIDTELIGDLPEEQRKEYLKMIPQKRFGKVE
                     EVAHAVLFLATREASYINGTSITVAGGL"
     gene            64230..64646
                     /locus_tag="PLR13_02535"
     CDS             64230..64646
                     /locus_tag="PLR13_02535"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HQC18238.1"
                     /translation="MDEVLAALPQRPPFLFVDKIICRDGRSITTQRTLRADEYFFQGH
                     FPGNPIMPGVLLCEACFQTGAILMVASHQPGLGVVTRIRDIKFKHFARPGDTLTMKVT
                     LDDQVDNAYYMSGTIHIGDKLILKIIFQAALLPPEQ"
     gene            64658..>65118
                     /locus_tag="PLR13_02540"
     CDS             64658..>65118
                     /locus_tag="PLR13_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC18239.1"
                     /translation="MDFLQVSGKTFLVTGVANKKSVAFFVAKGLVENGAQVIFSVQNE
                     ENKDRAQKLFPDSTVLICDVEKTGSINRLGEYFKNHNIKLAGFVHSIAFANYSEGLKP
                     FHETKPEDFLQAANISCFSLVAVANALKNSFENDASIVTISISNTRATAYG"
BASE COUNT        16368 a        16050 c        16433 g        16167 t
ORIGIN      
        1 tggataccga agggaatctg gtctatctgg cgttaagcga gtggcgcctc ggccacacca
       61 tggaagaatg gccgaaagtt cagttccata agacgatgga aaaaagctga tggccttgct
      121 cccccggtct tttttcaggc gcgaatataa gtttaccgcg cccgagtgct gttagtggcc
      181 tttttcgaag acgtgcggcg cgaccgaccg ttcggcgagc agatcaccca gcttgacccg
      241 catgaaagcg ctggtcgtgt agcgggtgac cttgtcatag taacggctga cgacgtgatc
      301 gaccatggcg gtgtacggtt cgatcaactc gggaaggata gcgaaattgt cgtagttgac
      361 aatcgtctgg acttttttcg gcagcagggg acggcaaatg cgctcgacat gctcgcggat
      421 ctcatcgatc tgttcggtcg atctgatgct caacccttca aaattgacaa agaaaaggtt
      481 ttcctccggc tggtaagtga aacgttcggc aaggggcagc gtgagcaggt cttctttaag
      541 gcccatggcc tccggacgaa aaatccgggc atccatcatc aggagttttt cagccatcag
      601 cggcgtgaat ttcattttct ccaggatgtc tttttgcagg ttgacgccgg gcgcgatctc
      661 gacgagcatc aatcccttct cgcttaaacg gaacacacag cgctcggtga cgtaaagaat
      721 ggtgcgtccg ctttgagcgc ccacggctcc gctgaacgtg atctgatcga cggcatcgat
      781 gaatttgggc atatcgccgt cgcttattat tttcaatttt tcatcagcaa cgtctacctg
      841 caagtttccg gcgacgaagg ttcccgcaaa caccactttc ttcgcgttct gactgatatt
      901 gataaagccc cccgctccgg ccaggcggga accgaagcgg ctcacgttga gattgccggc
      961 ccggtcagcc tgcgccagac ctaaaaaggc gatgtccagt ccgccgccgt cgtagaaatc
     1021 gaactggtaa ggctggtcga tgatcgcttc cacattggtt gccgccccga agctcaggcc
     1081 gcccgcggga atgccgccga tcacgcccgg ttccgccgtg agcgtcagat actcgagcaa
     1141 accttcttcc gctgcgatgc gagcaatgct ttcgggcacg ccgattccca ggttcacgac
     1201 agaattgact ttcagctcaa gcgcggcgcg gcgggcgatg atttttcttt catccagggg
     1261 cagcggctcc agagacgtta ccggcaccct ggtttcgccg gaataggcgg gattgtattt
     1321 ttccgcgaac gtcatccagt ggtcgtcggg atcggcaacc acgacgcaat ccacaagaat
     1381 gtcgggaatc ctgacttcac gggcgcgtaa cgtcccgcga tccgcaatac gctcgacctg
     1441 aacgatgacc acgccgcccg aatttctcac cgccatggcg atggacaggg catccactgt
     1501 caacgcttcc cgttccatgg tgacattgcc gtccaaatcg gcggtcgttc cgcgcaatac
     1561 ggctatctga ataggccgtg ttttataggc cagacagtct tcgccgtcaa agttcaacag
     1621 ttcaacgata tcgttccggg ttttccggtt gattttaccg ccgccgtgcc ggggatcgac
     1681 aaacgtgccc agtcccactt tggagagggt tcgcggatta tgcgccgcga tatcgcgaaa
     1741 catgtgtgag atgaccccct ggggaagatt gtaggcttca atcttgtttt cgatggcgag
     1801 cttctgcagt ttgggaacca gtccccagtg accgccgatg acccggctga ccagtccttc
     1861 atgagcaagg tgattcagcc ctttgtcttt gccgtcgccg attccggcgg aatagataat
     1921 ggtgaggtct ttgggttttc cctgcgcgat aaattgtttt tcgatggccc tggcgacgcc
     1981 ttcggcaaag ccgatgccga caaatccgcc gaacgcgagc gtgtctccgt cgcgaatgac
     2041 gcccaccgcc tcatcaagcg aaactatctt gccgcgcttc atgcgccggg ccaggtcgct
     2101 ggcgatcgga tgacggctct tgatcggatg attcatcatt cactcggtgt ttgcggaggc
     2161 aatttttact cccggtaaat cgcctcgaaa attacttcaa ggcccgtgtc acgaatatcc
     2221 cggcaggcat cgagaaatgt ggcgatgttg tcagaatgcc gccgctcgat gtaactgaag
     2281 gtgttggatt cctgcgcccg gcttcggtta tccttgaccc atgacgccca ggagtcgaaa
     2341 atcttcatcc cgggactcac ctgatccgac cacacataac ggctgacgcg cgccggatga
     2401 agccagcgta aagccgctgc ctgctgcgga tgccggatca gcgacttcac gaagggccgg
     2461 ccgaaagcga gataaagatt ttcgttggcc accgacaggc ggtccatttt atcaaatgcg
     2521 gatttcggaa tcggacaggt aacgtctttg atttcccttt tttcaaagcg gacacggaac
     2581 tgctcttgat gcgggtcgtg atcgcctgtc tcgccttcaa tgatcatttc atacaggccg
     2641 ggctttaatt tctgcatctt ctcgatatgt tcgataatcg cacggtgctc gcgtcgcgcc
     2701 acggaggcgg aaacaaagat gccgagatgt ccgatatgac tatgcaacag atagacgatg
     2761 cgctgtccgg cctttacaag atcgtccgtt gtcgggtaca cttccgaaat ccagtgcagc
     2821 gcctgttgcg gcggcgtgat gttgtcgccg ctggacgcga agatgacgag cggatccttc
     2881 atgttcctca tgtcaatccg gtgaccgtgg ccgatatcca gcatcccctt ctccaacttg
     2941 ttgccgatga acagatttct tacaatccag aggatttcct cctcgttgag cagataaaag
     3001 ccgccccacc agcgttcaaa ctcgaggtaa cgtttctgct cggtgtcaat attggaaaaa
     3061 aggttgtagt cttttttcca gagggtgttg gccgggttga gccgctcgaa attctccacc
     3121 agataggcac cgtcaaaaag acctccgccc agatcacaca tgaaactggt cgcccaggtg
     3181 ccgccggtca atccgcccgt cagacgcatc ggattaatgc cgtcaccgcc cgcccagtag
     3241 gacatgggcg cgccgttgat gacaatcggt ccggccacgt cggggcggtc cgctcccagc
     3301 atggcgacgg cccatcccgc ctgacagttg ccgtaaatca tgggaaaatc acggtccgga
     3361 tgacgttttt ttatttcttc cagaaaacgg atctccgcct gttcgacatc ttccatggtc
     3421 tgatccggaa gcggttcggg tgtaaaggat gcgaagtaaa ccggatgacc ttccgcaaga
     3481 cccatgccca cctcggaatc ttttttgaat ccgccgattc ccggtccgtg cccggctcgc
     3541 gggtcaataa ccagaatggg ccttttcccg ggtttcggtt ccttgccttc cgggtgtctg
     3601 atgtacagca gcgaatagtt gaccggacgg ggaaacttgc gtgcgtccag aacgacttcg
     3661 aattcatagt ccagaagagg cggcatgccc tgctgataat gctccagcat ggtgttgccg
     3721 cgccggcgca tggtatccat gaaaagaacg caacgctgcc agaagtcgat ggaatactcc
     3781 caggcatctt cggaaaatct tgcccaggtt aaatgcgaca gcctggtgcc caaatccacc
     3841 catgaagcca gtgcgccgag cggctctacg ggggcctctg atttgatttt tttattgcag
     3901 gccatgtcgg ttgtctgtgt cataagttga tgccatgatt tgaaaaggtc gtaatgattc
     3961 attttgttct ctcaggaatg ttttaaggtg atcatgataa aaaatctgtc tctcactttc
     4021 aagcagtata ggaaactcac cccgggttgt caatgaaata ttaaccggat aagttattaa
     4081 atttatttat tttaagccaa tgccggttta atttttttcc ggatgaaccg tagcgcggga
     4141 attgaaggaa accggattcg ttcgttcttg attgcgtcaa aatattcatt gaagtttttc
     4201 ctattttaac ataactgatt gtcagcttgc atccgatttg agtcatgatc cgaaaaaagg
     4261 ggaggccgta gcgtacagtt tccgtttttt tccgtgcata gaaagcttgt aggaaaagga
     4321 ggtaaaaaat gaataacggc tcaaccaaaa tgacggatat taagatagac accaacaacc
     4381 tttataagga agaagctttc accgacctga cgtttgccac catcaggcgg ctcaatcctg
     4441 tgaatctgga cggctcgcct gacgccagcc gcgaaccgat ttttaccggt atgacccagt
     4501 tgatgtcgcc caacggtccg attcctgttc aatgtcttat cgaaggagca aagacgcttg
     4561 ccgaagctgc agaaaaatta ccggtggcca ttgaaaaaac ggttcaggcg atgatcgccg
     4621 aagccaagga aatggaacgt gaggaagctt ccaagatcgt catccccggt cagtcatgaa
     4681 acaaaatctg ccggacaaaa atccggcaga ttatttgagt cttttgtcca ttcccgtaaa
     4741 accgcaatcg ggctgatagc aggttacgtt cagaaattcg catttctgtt tgcaaaccgg
     4801 acacgtttcc ggtggtgtaa tttgcaccac cgtattgccg caattactac acttccaggt
     4861 attttcggga agaggctgtt tgttctctgt cattgccgta ttttctcctt atctgggtta
     4921 aggtgattct atcacaaaaa acagatagat agacattttt tctttttccc gacgaatatg
     4981 cttgatatta ttacccactt tcggtaaacg aatataccat ttttacccaa ggcaaaaaca
     5041 tgcccaaaga aaaaaagaaa aatgattttg caggattaac actgctggga aaggcggcaa
     5101 agccggtgcg caaactggag acatttccca atcacaacga aaaacgtgcc tacgttgtca
     5161 ccctgcagac ggaagaattt acctgcgtct gtcccaaaac gggccagccg gattttgcca
     5221 aaattaaaat ccaatacata ccggataaaa aaattgtcga atccaaatcg ctgaaactgt
     5281 atttatggtc cttccgtaat gagggtgttt tccacgaaca tgtcaccaat atcattctgg
     5341 atgatctggt agcagcgctg aaaccccgct ggtgcaaagt cagcgcggaa tttgccgtgc
     5401 gcggcggcat cggaatcacc gttgacgcgg aatataaaaa ataaccttat gaggaaaagg
     5461 aaaatccatg ggaaaaagaa cggatattaa aaaagttttg atcatcggtt cgggcccgat
     5521 tgttatcgga caggcctgtg aatttgatta ttcgggaacg caggcgtgca aggcgctgcg
     5581 aaacctcggt tatgaaatcg tgctggtgaa ttccaatccg gcgaccatca tgaccgatcc
     5641 ggggatggcg gatgtgacgt atatagaacc tctgaatatc caggcattaa cggaaattat
     5701 tgaaaacgaa aaaccggacg ccatcctgcc caatctgggc ggacaaaccg gccttaatct
     5761 gacgtcggaa ctttacaaaa agggcgtgct ggaaaaacat aacgtgaaag ttatcggcgt
     5821 gcaggttgat gcgatcgagc gcggcgaaga cagaatcgcc tttaaggaaa cgatgaacaa
     5881 acttggcatc gatatgccca aaagcgaacc ggccacctcc gttgaagaag cggaaaaaat
     5941 cgccacaaaa atgggttacc ctgtcgtggt ccggccggct tacacaatgg gcggcaccgg
     6001 aggcggcatt gtctataatc tggaagagct gcgcctcatt gccagccgcg gcatttcggc
     6061 cagcctcatc ggccagattc tgattgaaga atcggtcatc ggttgggagg aactggaact
     6121 ggaagtcgtt cgtgacgcga aaaaccagat gatcaccgtc tgcttcattg aaaacgtcga
     6181 tgcgatgggc gtgcacaccg gcgattccta ctgcaccgcg ccgatgctga ccattactcc
     6241 ggaattgcag aaacgccttc aggattattc ctacaaaatc gtcgaggcca tccaggtgat
     6301 cggcggcacc aatgtgcagt ttgcgcacga tcccaaaaca gaccgcgtgg tcgtcatcga
     6361 aatcaatccg cgtacgtcgc gttcctccgc gctggcctcc aaagccacgg gttttcccat
     6421 tgcgctgatt tcctccattc tggcttccgg aatgacgctg gatgaaattc cctactggcg
     6481 ggacgggtcg ctggaaaaat acacaccctc gggcgactat gtcgtcgtca aattcgcccg
     6541 ctgggatttt gaaaaattcc ccggttcgga agacaagctg ggcacgcaaa tgcgcgctgt
     6601 cggcgaagtc atgagcatcg gcaaaaatta caaggaagcg ttacagaaag ccatccggtc
     6661 gctggagaaa aaacgttacg gcctgggatt tgttaaagac ttcaacgcga aatcgttgga
     6721 tgaattgatg gaaatgctct ccgagccctc cagcgaacgg caattcatca tgtatgaggc
     6781 cctgcgcaaa ggtgcttcgg ttgagacgct gtccgccaaa acccatatta aaaaatattt
     6841 catcgagcag atgaaggacc tggtcggcct ggaggaaaga attcttcagt ataaaaagaa
     6901 aaagatgccc gatgagctgc tgacgcaggc gaaaaaagac ggctttgccg ataaatatct
     6961 ggcacagatt ttgggcaaag aggaagaggc gatccgcaaa cagcggcagg cgttgggcgt
     7021 aacggaagcc tgggaagccg taccggtcag cggcgtggaa aatgcagcgt attatttttc
     7081 cacctacaac gcgccggata ctgtgcctgt aaccgggaat cgcaaggtaa tggttttggg
     7141 cggcgggccc aatcgaatcg gccagggtat tgaattcgac tactgctgcg ttcacgcggc
     7201 atttgccctg cgcgatgaag gtatcgaatc catcatggtc aactgcaatc cggaaacggt
     7261 ttccacggat tacgacacct ccaataaact gtattttgaa ccgctgacag tggaagatgt
     7321 tttaagcatc tacgagaaag aaaaaccgga aggggtgatt gtgcagttcg gcggacagac
     7381 cccgcttaac atcgcggcgg aactggccaa agccggtgtg aaaattatcg gaacatcgcc
     7441 ggagacgatc gatctggcgg aagaccgcga ccgtttccgc atgatgatgg ataaactggg
     7501 cattcccatg ccgaaatccg gcatggccag caatctcaag caggcgctga aagtggccgg
     7561 tgacatcggt tatccgctga tggtgcgtcc ctcctacgtg ctgggcgggc gcggcatgga
     7621 gatcgtacat gatgaggaaa tgctcaaacg ctatgtcaac gcggcagtcg gcgtcacgcc
     7681 ggagcggccg attctgatcg acaagtttct ggaaaacgcc atcgaagcgg aagccgacgc
     7741 gatttcggac ggcacggatg cttttgttcc ggcggtcatg gaacatatcg agctggccgg
     7801 tgttcattcc ggcgattccg cctgtgtcat tccgccggtg agcattccgc tgcgtcacat
     7861 tgaaactatc aatgactata caaaaaaaat tgccgtggaa ttcggagtaa tcggcctgat
     7921 gaacatacag tacgccatcg ccaatgatgt ggtttacatt ctggaggcga atccccgcgc
     7981 ctcgcgcacg gtgccactgg tttccaaagt ctgtaatgtg tccatggcgc gtctggccac
     8041 gcaaatcatg ctgggcaaaa agcttaagga tctgaatctg aaaaacaaaa cgttccgtca
     8101 cttcggcgtg aaggaggccg tttttccctt taacatgtat ccggaagtcg acccggttct
     8161 gggcccggaa atgcggtcca ccggcgaagt gctgggactg gccgattctt tcggcctggc
     8221 ttattacaag gcacaggaag cgactcagca aaaccttccc tcggagggaa ccgttctgat
     8281 tacggtggaa gaaaaagaca agggcggcgt gctggaagcg gcgcgggcgt ttaaaaaaat
     8341 cggttttcaa attcttgcct cggaggggac gcacaggttt ctgaaagaaa acggaatcga
     8401 ggccgaaaag attcttaaaa tgcatgaggg caggcccaat attgtggatg ccatcaagaa
     8461 cggcgaaatt caactggtga ttaacacacc ggccgggcgc ctcagcaaat atgatgattc
     8521 ctatatccgt aaagcagcca ttaaatataa aattccttat atcacgacca tcgcggcagc
     8581 ggtcgcggcc gtcaagggaa ttgcagcgct gaagcagggc cacggacggg caaaatccct
     8641 gcagagctat cacgcggaaa ttcagtagga atcaggctcc gatttgagaa aataaagctt
     8701 ttatttttcg acagctcatt ataggttaat aatatataaa agaacattgt ttataaaaaa
     8761 gctgtgggca aacttttaat gagacacggg cgaatatctt ccgtatattt acattgactt
     8821 attaaaattc ttcctttagc ttcattcaca ttataacaat ccgatagaat tttgagcgga
     8881 cattgaggct gtatagacga ctttgcgtga ggagaaggaa tggaaagcaa ggacggtaaa
     8941 gaagataaaa acgttaacgc cttactctct ctccctgaca gtaccaagac gttgggaaaa
     9001 aaatccaagt ccagagaggt caacccttcc ggattaaaga ggaaggtttc gcaggatacc
     9061 cgcaggatgc cgcagaaaga caaaccggcg atcagcggcg cgtccccaaa gacggccgga
     9121 cataaaaacg ggaaggagtc tttttgcggc cctgaagaaa aatacgcgct ggcgttcaaa
     9181 tatagtccga tcgggatctg catatccagc ctgcaggatg gccgctatgt tgaagtcaac
     9241 gaggtttaca gtaattttct gggataccgc cgcgaagaac tcatcggacg cacctcttcg
     9301 gaaatcaatc tctgggtgga tatggccgaa cgcgacgcac tggttcggga acttcttgaa
     9361 aaaggcaccg tgacggattt tcaattgcgg tttcgtgaca gaaacggcaa cgttcactgg
     9421 ggaaatgtgg ccgccgcact ggtgaccatc ggacaggagt tgtatgttct cacacagacc
     9481 caggatgtca ccgataaaaa gaagatcaaa gaacagtttg agaaaagtga ggccaagtac
     9541 cgtctgctgg cagaaaatct gaccgacgtc atcttcacca tcgacatgaa tttcaattat
     9601 acgtatgcca gtccttccac gtatcatctt cttggataca cggcagaaga attgatgacc
     9661 atgaaagtgg agaatcttgt tgatcctgaa acgctcgcct ggttcatgga gatgctggct
     9721 gaggagatgg aaatagaaaa gcggcccgac cgggatttga aaagatcgcg ggttcttgaa
     9781 tatcagcata tccgcaagga tggatcaacg atatgggtgg aaaacaagat aacgttctta
     9841 agagatcaag acaataatgc gataggaatc atcggaaccg tccgcgacat tgccgaacgc
     9901 aaacgggcgg aagagcaggt ttgtaaagaa atcgatttca accgttccgt actggacagt
     9961 ctgacggtcc ctttcttcat gtttgatacc gccagtatga ctttcttcag atggaacaaa
    10021 accttttccg ctgtttccgg atattcggat gaggaactcg gccaaatgag accgtcggct
    10081 ttgatatcgg aacaccagaa agatttttta ttggccaagt tcgaggaatt tcaaagaaca
    10141 ggccatatcg cctttgaaat gccggttctt tccaaaaacg gttctgtaac accgtatttg
    10201 ctttcgggca atcttctgca tcacgacggg aagccttttg tggtgggtat ggggattgac
    10261 attacggagc gcaaaaaagc ggaagaatcg ctgcgccgga gcgaggaaaa ataccggacc
    10321 attcttgaga acatggagga agggtatttt gaagtcgacc tttccggcaa ttttactttt
    10381 ttcaacgatg ccacctgccg gcatttcggc tattcccgcg aggaaatgac gggaatgaaa
    10441 aaccgtcagt atatggacga aaaaaattca aaagcgcttt atattgtttt taatcgtatt
    10501 tacgaaacgg atcagccggt aacaaatctg gaatggcaga tcataagaaa agacggcgcc
    10561 aaaagagata tcgaaggttt tgtctttctg ctgaaggatg ttgcaggcac cccgataggt
    10621 ttcagaggaa ttgtccggga tattaccgaa cgcaaacagg tgcaggaaaa gctgcaccgc
    10681 gaaaggcagt tattcaaatc tgttacggaa caatcgtccg acatcattat tattgccgac
    10741 acgcgaggaa ggatcactta cgaaaatccg gctgttgaaa agatactcgg ataccgtgtt
    10801 gaagaaaggc tgggggcatc gggatatgac ctggttcatc cggatgatgc gcaaatcgtc
    10861 gctgatgctg cggggaaact tttccacaat atccaagcgc ctgtgcagaa aggcgaaata
    10921 cggttgcatc ataaaaacgg aagctggcgg acgtttgaag tcacggccag caatttggtc
    10981 aaggatcatg tcgtggaagc cgccattgtc aatatgcacg acatcaccgc gagaaaacgg
    11041 tcggaggaac tcatcaggca aagtgaagca aaatatcgtc tgctggcgga taatatgaaa
    11101 gattacgtgt ggctgatgga tctgaacctg aaaatcaatt atatcagccc ctctgttgaa
    11161 aggttaatgg gttattcgct tgaagacttc aaaaaattct ctctcgacaa atttttgacc
    11221 gtacaatctt tccaggcggc gatggatttc tttgccattg aaatgcctaa agctctggga
    11281 gctcctccgg attatgtttt ggagcggtct ctggaacttg agttttgctg taaaaacggc
    11341 caaaccgtct ggggagaatg caatttcagt ttaatcagag atgacaaagg caaaccgttg
    11401 tcccttctgg gggtcagcag gaatattaat gaacgcaagc aaatggaaga tgctctgcgg
    11461 aaaagcgaag aaaatttccg tcattctctc gatgactctc cgctcggtgt ccgtatttcg
    11521 acggttgaag gaaaaacgct ttatgccaac agggctatcc tggacatgta cgggtatgac
    11581 agcaaagagg aattgcagaa cacgccgctg caggagcgct atacgcctga gacgttcgaa
    11641 gcgttcaaag aaagaaagaa gaaacggctg aagggccagt tcggtccgtc cgaatatgaa
    11701 atcagcatta tcagaaagga cggagaaatc cgccatctgc atgttttccg caaggaaatt
    11761 ttctggaacg gccagaaaca gtcccaggtg atttatgagg atataacgct tcgcagacga
    11821 gcggaaaaca gactgaatga aacgctggaa agtctgcgtc aatccatcaa aataaccatt
    11881 caggtcctgg ggacggcatc agaggcaaaa gatccttaca tggccggcca tcaaagacgg
    11941 gttgccgatt tggcccgtgc tattgccacg gaaatgaaac ttcctcagga taaaattgag
    12001 gcgatacgaa tggccagcgc cattcatgat atcgggaaaa tttcggttcc ggcagaaatt
    12061 ctctgtaaac ccgcgattct cagtgatatc gaattttctt ttgtgaaaaa tcatccccgc
    12121 tacagttatg acatcatgaa agaagttgaa tcgccctggc ctttggccga tattgtttat
    12181 caacaccatg aacgcatgga tggttcggga tatcctttgg gactgaagaa tgacgaaatt
    12241 ctcatcgagt cccgtattct cgccgttgcc gatgtggtgg aggccatgat atcgtaccgt
    12301 ccctacagac cggcgctggc gcttgaaatg gcgcttgcgg aaattgaaga taatgcgggc
    12361 actctctatg acgccgaggt ggtcaaagcc tgcctgagat tatttcggga caaaaattat
    12421 caaatgtctt aagatgatat atacgtaccg gatacaaatt gaaaaaacgg cgattccacg
    12481 gatacgcttg acggatcgat ggattgtttt ataatatccc atgatttatg aaggaggttc
    12541 cctgtgaaaa aacttacggc cattattttg tttgtgcttt gtctgatccc ggttgtgata
    12601 tttgccaaaa acgcatcggt ggaggaccag ttcaaaaaaa gttttccgaa aaacaagttt
    12661 gaaagtatcg tacccacatc catcaaggga atttatgaag tgtatagcgg caatcagctt
    12721 tactattaca tgccgaagga cgagatcgtg ctttacggca ccctggtgtc caaaagcggc
    12781 gtcaacatca cccgtgatag tcttttaaag aagatggcgc agaagatgga gaaattgcct
    12841 ctggagaacg ctctcaagat cggtaacgga aaaaaaatca ttgttgaatt tatggatccc
    12901 cattgttacc attgccgtca ggcctatcag ttcttttccc agcgcaaaga tgtgaccatg
    12961 catgttttct ttttcccgct gtccaaagaa tccgcagatc aaatcaaaca tatttattgt
    13021 tccaaggacg ttttaaaaac atatgacgat gtcatgaaag gcaagctcga caataacgcc
    13081 agtctgaatg tgtgcgcgga tgccaaaatc gatgaaaaaa taaagacgca tgtgcagctt
    13141 gcgtcgcagg tcggactgcg gggaacgcct ttattctaca ttaaaggcca ggttgtggac
    13201 ggatttgatg ttgcgctgat cgagaaactt ctcaaagact gattttcata aggccggccg
    13261 ctcttgctgc gggagagcgg ctagcggatt tttcctcctg tttttgccgc gcggtcgaac
    13321 gtcagaaggg ttaatccttc tggcgtgtcg gtggccagca gcatgcctcg ggattctacc
    13381 cccatcaact ttgccggttt taaattcgcc acaatcacga tttgttttcc gataaggtct
    13441 tccggtttgt aatccttgcc gatgccggcc acgacggtgc gttcttcgcc gatgtccacc
    13501 gtcagtttta caagtttgtt ggatttgggt acggcgtgag ccgcgagaat cgttgcggcg
    13561 cgtaaatcca cttttgaaaa tgtttcataa tctatttccg gcttgaggtc ggtcatggct
    13621 gctttctgat cctcgggttt ttctttttgc tgaacaatac gcgggaacag ggcatcgctg
    13681 cgggtcagga cctggcctgt tttcaatgcg ttgtccgttt tgatgtcgtc cagtttcaga
    13741 ccttcggttt tttcgcatcc gatctgtcgg agaatctttt ccgccgtttg cggcatgaaa
    13801 ggcgaaagca tgacggcgat cgcgtgcagc gccgaaagca gatcatacat gaccgccgtt
    13861 aattttttac cgttggccgg gtctttggcc agcgaccaag gctcgttttc gacgatgtat
    13921 ttgttggtga tattaatgaa atcccagacg gcaatcaggg ctttgtgcag agacatatcc
    13981 ctgaaactcg cctcaacgtc taagacggct ttcaaagcgg cctcgcgaag ggccggattt
    14041 ttttcatcgc ctgcaacggc cggcactctg ccgccgcaat atttgatggc catggtaatg
    14101 gcgcgactga ccaggtttcc cagatcgttg gccagatcgg aattcaggcg accgacaaaa
    14161 gcatcctcgg agaaattgga atccagaccg aaaaccatgt cccttaaaag aaaatatcga
    14221 aacgcatcca gaccgtattt atccttcaaa tccagcgggc ggacgatatt gccgaggctt
    14281 ttggacattt tgctctgatc ggagttccag tacccgtgca cgttcaggtg ctgataaggt
    14341 tcaatacccg ccgctttcag catcgtcggc cagtagatgc cgtgcggttt taagatatcc
    14401 ttggcgatga ggtgttcggc gaccggccag aaggtcttga aatgctcccc ctcgggatac
    14461 cccacggcgg tgacgtaatt aatcagggca tcgaaccaga cataggtaac atactgctca
    14521 tcaaacggca gggtaatccc ccattccagt ctggttttgg ggcgggaaat gcaaaggtct
    14581 tccaggggat cgcgtaaaaa agccagcgct tcatttctgt atctctccgg ccggatgaat
    14641 tcggggttct ttttgatatg gtcgatgagc cagtcctgat atttgctcat tttgaaaaaa
    14701 taatttttct cttcaatata ctgaggttcc cggccgtgtt ccggacattt accgtcgacg
    14761 atttcctttt cggtgagaaa tcgctcgcag ccgaaacagt agtaaccgcc gtaactcccg
    14821 aaataaatat cgccggcatc gtacaccttc tgaagaatat agcgcacggt ttcgatgtga
    14881 ttggcgtcgg tggttctgat gaaataatca ttcgagatgg caagttgcgg acaaagggcg
    14941 cgaaactggg cgctgatgtt atccgcgtat tgtttcgggg ttaatccggc tttctgcgcc
    15001 gcctcggcga ttttatcgcc gtgttcgtcg gttccggtaa cgaaaaacgt tttatctccg
    15061 atcatgcggt gatatcgggc cagcacatcg gcgacaatgg tcgtataagc atgcccgata
    15121 tgcggtgaag cattaacata gtaaatcggt gtcgtgatat aaaatgattt cggcatatgt
    15181 gacgtttctc cattcatgac tgaagatgat caatgtcatc catcttttcc agatccagcg
    15241 tttgcttttc ttcctgtgct tcagtggcgc tgccggacgt tttggccgct ttggcggctt
    15301 ttttctttcc ggcatagccg tcgtattcga aggccaggca gcacatcagc cttccgcaca
    15361 ggcctgaaat tttttcagga ttcagcgaca tgctttgttc cttggccatc ttgaccgtga
    15421 ctcgttcgag attttgcagg atgccggcac agcacaccat ccggccgcaa attcccagtc
    15481 ctttggtaat ccgcgtttcc tgacgggcgc cgatctgttt gagctcgatt ctcgttttga
    15541 acttctggac cagatccttg acaagttccc gaaaatccac cctgctgtcc gctgtaaagt
    15601 aaaaaataat tttgtttttt tcaaaaaggc agtcgacatt caccagtttc atggaaagct
    15661 cgcgtttgcg aattctttcc agacagaatt tccaggcctc ctgttccagc gcgtcattct
    15721 cttttctgat ctttaaatcg tcgtccgtca ccttgcggat tatttttttg agattggccg
    15781 gaacggccga cggctcgcat cgcttcacat ccgtgacgac agtgcccagc gccaggccgc
    15841 tgtcggtttc gacaagcacc tgttcatccc ggtgcaggat aagatcggcg gcgtcaaagt
    15901 tatagatccg gccttctttc ttgaatttaa ctccaatgat atttacaagc aatgtccatc
    15961 cacccttttt taataattca gcttaaatgc cattgtttcc aatgtcaatg atttgttgac
    16021 gttttgttcg atgatgtccc ccgctttttc tatctgcgca atgttttgca gaatctgctt
    16081 tccggtcaat ctggccgcgc aggccgcaat cagaggcgta ttgtcctgat tgatcagcat
    16141 tcctttctgg ccgacttcct tgaaaaccag cacatcgcga aagaacgtgt tcagaatatt
    16201 caggctctgc cggatttcct tttttttctg gcccagaaaa gaggcaaaat ggatcaggct
    16261 gaaaggctct ttccggtcgg tcagggccag cgtgttgaac agttcggtcc ggtacgcgac
    16321 aacttcctct tggttgagct ccagcgcgtt gccgattgtt ccgccggata acgcggccag
    16381 cagcattgcc cttgaatgct ccatgttttt ttcggatacg agaaaccgcg ccaccgtttc
    16441 gacggccagc ggattaaagt ggatgtgctg gcagcgggaa atgatggtct gaagcatagc
    16501 gtgcggcctc gttgtgatca gaatcagcat attggccggc gacggttctt ccagcatttt
    16561 caaaagggca ttggcggatt cctcattcat tctatcggca tcatcaataa taaaagctct
    16621 ttttttggcc tccagcggcc ggcaggtggt tcgctcctgg atctgacgga tggaagcgat
    16681 gcggatgaac tggccctcgg gttccacaaa aaaaatatcc ggatgactgg catgttgaat
    16741 tttgcggcag gacggacagg catcgcaggc atcatgccgg tcatccgaac ttatgcaatt
    16801 gacggccttg acgaattcca cagccagcgt tttcttgccg gtcgccgaag gaccgctgaa
    16861 gagataggcg tgcgcgattc gccggcgggc gagggttttg cggagcatgt caattttttt
    16921 ttcgtgaccg taaatattgc cgagcgacat aatgcctgaa aaaattcttt taaaaagatg
    16981 attttagacc ttgataaaac tgcttatgtg acgacgcacc tcttcctgaa tgaccggaat
    17041 cttccgggtg gcgtcaatca ggcggaaacg ttccggttca ttatttaaaa tagtcagata
    17101 cccttcacgg atgcgacggt gaaaatccag agtctccctt tcaaaccggt cggtggcggc
    17161 cgggtcggcg agtcgattgt tgcgcgccgt tgccctttcc agtccggttt ccaccggcag
    17221 gtcaaacaat aaggtcatgt ccggtttcaa ctgcatcgcg gaataatcat tgaccgcgcc
    17281 gatgaatttc tgatcgaggc cgcgtccgaa accctgatag gcgtaggtgg cgtcggaaaa
    17341 gcgatcgcat aacacgagtg tcccctgccg gagggcgggt agaatgacgt cccggacatg
    17401 ttgggcccgg gcggcgcaga ataaaaacat ttctgcttct gcgcacagat gattgtgcct
    17461 gcggtggaaa agaatgccgg cgatctgtcg cccgatgtcc gttccggaag gttcttgcgt
    17521 gacaatcagt tcaacgccct ggttttgcag atattcggcg acgagcttga tctgcgtcga
    17581 ttttcccgaa ccttcgatac cctcaaaggt gataaatttt tttgccttga tcatgtgact
    17641 ccgctttcac ctgcgcaacg gcgcaatgaa ccggtaaccg tgttttaaaa aataaaaccg
    17701 ggaatgaatc aatgcgtaaa agcagattta ttcccggagc tgatttttcg tctgtgaatt
    17761 atttcccctc tccgggaacg atcgcttcta ccggacactg gtcggcgcag gttccgcagt
    17821 cattgcacag tttaggatcg ataacatact tgtcatcgcc ctctgagatg gctccttccg
    17881 gacattcgct ttcacaagaa ccgcaagcta tacattcgtc agtaatcaca tatgccataa
    17941 ttttatgctc cttttatcgt tattttaaaa tacacctttc ccgctttgtc ttgtcaggaa
    18001 acgattgttc ttaaaaagcc actttcccgt tgctgatgtg cgaaacggct cagacggcca
    18061 cgatctcttt aactccgggg accagtttct taatgacttt ttctattccc atttttaacg
    18121 tcatctgcgc catggggcat ccgtggcagg cgccggttaa cctgaccttg actgtgccgt
    18181 catcgctgac atcgaccagt tccacatcgc cgccgtccgc ctgcagactg ggcctgatgg
    18241 tatcaatggc tttctgtacg tcttctttca tggaggttct ccctattccg ttttttctta
    18301 tttttcaagt ccttcttttc aatgcggcat gtaataaacc attttgtttg cgacatcccg
    18361 ggccatatca ggcaaacccg gttttattaa tacataatcc ttcttgatgg tgtgattggc
    18421 tttccacatt aatttgccgt tcgtaacatc atacagctcc attgtcaaac tgacctgcgc
    18481 cattttctta tctgcctcaa cgaaatattt ccattcgtca acggataaca gcagaatacc
    18541 gtcaacctgc aggatgtcac cgattttcct acttaaatca gcatcggaaa acttcagaag
    18601 ttgcagtttg gaaaaatact cttccgctgt ttttttcaat agttcatcat tttgcatctg
    18661 tttttgcagt ttttccgcgt ctgtgacaga agtaaacaat ttcttggcaa tcagtgagcc
    18721 ggccacaatc tgttggacca ccgttctgtt atcgggttct ttgtgattcc agacttcaat
    18781 cggaaaaaca gcaatactct gcggatgaaa atcgatggcc tccggcgcca gttgagaaaa
    18841 gtgcaaaccg ccgcaaccgg caatggttag aataagggca atccatacaa agacataaga
    18901 ttttttttta atcattgaac aaaaaccgcc tcgtcatcag atcagaatag ccggtgtcag
    18961 aaaatcagcc acagcaaaac acaaaaacca gctataatgt agctggtttc cagaaggcct
    19021 tccttaaccg ctccagaaag cacgggtctt ttatcacaaa acaggaaaca aatccacaga
    19081 tataataaac aaaacatcag cggaaaactc agggatgacg tccatcctga aggcgatgca
    19141 aaaagtaaaa tagcgaataa tatcaatgag ataatcttca ggatattcaa ggcgcgtgtt
    19201 ttaccgaaca gcacgggaaa cgtttccttg ccgatcagtt tgtcgctttg tatctccaga
    19261 atgtccgaca tggctgaacg aataaaaacg atgcagaaaa caaagaaaaa tgcaaccaaa
    19321 aggccggcat ccggaaaggg ggtctcatgg atagagggta aaaacgcagc ggccaccgcc
    19381 caggctatgg acataaatat gttttttgaa ccgggcacat ctttaagact ttggaagttc
    19441 caccttgggg aaagcagttg catattataa agcacgcccc ccagtgaaat gatcagcagg
    19501 gaaaagaaaa cagccagcga ttgctgaaag gccagtacca gcgcaagaat aatggaaaat
    19561 aaggaagtga caaaaaatat attctcgtgc cgtatataat attcttctct gaaagatccg
    19621 acgagccccg ccggtttacg gctgatcaga cggtttaaga catgcatgcc gtaaacgaaa
    19681 agagaggcaa tcagaataaa tgaaaaacgg acggtcatgg tctgcagcaa catgcagaca
    19741 aggcacaggc aaccggcgcc gatggcggag aaaatatccg ttttaacgag gaatgtccaa
    19801 agagtgagca gttttcccag atttttcaac ttacggcttc gtccttccgt gattttgtct
    19861 atgactctgt ccagaatcca gttgggcgtg gacgccccgg ccgaaacacc gattttatta
    19921 tacgggccga ggtcgatttt ttccatatct gccggtgttt caatatgaaa tgttgagggt
    19981 tggtttttgc gggcgagatc agccaaccgt ttggtattgg cgctgttttt accgccgacg
    20041 atgaacatcg catccatctc accggccatg tcaatcactt ctgcttgtct ctgttcggtg
    20101 gacaaacaaa ttgtattgaa caccaccgcc tgcggacaga gttgtttgat cttttcgacg
    20161 atcttttgat actcgtcaag gttttgcgtt gtttgggcaa ccacgcagac cttttccagg
    20221 caaggaattt tattcacttc ctcttctgtg gaaataataa tgccccggcc gtcggtatac
    20281 cccagcaggc cgtcgacctc gggatgttcc ttatccccga cgatgacaac cgtgtatgtc
    20341 tgtgcggcat gttttttgat aatggactgg acgtaaccga ccttcgggca cgttgcgttc
    20401 accactttaa tgccggtttc tttaattttt tttctttctg ccggggcgat gccgtgcgcg
    20461 cgtatgatca aaatggcatt ttctttatct tcgatttcat caatatgggt aatcggtttg
    20521 atgccgcggc ttttcagcag ttcgatcgtc tggggattgt gaatcagcgg cccgtaggta
    20581 tagatgcggc ggtttttttc atgctgggct atgtccagta cgatatccac agcccggcgc
    20641 acccccatgc aaaagccggc agttttggcc agtctaattt ccatattgct ttgtctttcg
    20701 cgctattttg ttgttgtacg atatttgatc atcttcctga aacgagtccg gatgaccctg
    20761 acgttgccgt ttttctgccg gcccggatcg acccgagcgg caggacagga atccggcaag
    20821 cgccgccaac gaaaaaacgc ctcccgtcat aatggtgacg aatatgattc ccgtgttcgc
    20881 gagaaccatc catgatttga tgctgccgac cgtaatgatc gaggcgtaaa tcagacacgg
    20941 cagaaaaaaa tcgcggtcgc gggccgcaaa gcggtttctg tttctaaaaa atatcattct
    21001 atacgggata aagatcataa tcgtcgtcag aagccatccg aaatagttct gcgccggaat
    21061 gccgtagaag acggaaccgg acggcagtgc ttgcaccgtc gtccctgtcc atgttccccc
    21121 cgtcacccac aagggatcga tgagcacatt cagtgcggtc attgccgcgg catcgaaaaa
    21181 cgcaaacaaa aacggggcgg accggcctgc aaaccggtca ccgaaagccg catcaactgt
    21241 gaccgttgaa acatacatga acataaacca ggaaaacggt atcagcagcg gaaccgtatc
    21301 gaaaattttt atccccagcg cgtcgccgta atggtattcc ccgtaaataa ggccggtttt
    21361 cactcctgtg tattccataa taaaagatat gacaaatgta aggacggcca gagagatgat
    21421 acatttcctg ccgacgtatg taccgccgtg aagaagcgca aagcacgtga agaccgcccc
    21481 catggccata ttataataag gcggcgacgt tcttcccgac gcggtccata aaagctcaga
    21541 ataggtggaa aacaatacga tgatgaatag cacataaatc aaatgtttat ttttcatcag
    21601 cttccttttc cttctttcgt gtcatcgtct gacctgcaca ttgtcaggtt tgagacggtt
    21661 ttttccgcgt taagacgaaa aattccttgt ttcccgccgg gcccgaaatt ggcgaggcgc
    21721 aggtgccctc aacgtccaga tccaacgata tgcaaaaatc gctgatgttt tgcaccacgc
    21781 gatcatggat tgtgggatcc cggacaacgc ctccctcgcc gacgtctttt tttgatgctt
    21841 caaactgcgg tttaatcagc gccagaatcc gtgtgccttc ttccagaaat tttaaaaccg
    21901 gaggaattac agttttcaaa gatataaaag atgcgtcgat cacagccaga tcaatttttt
    21961 catccagatc cgcaccgtca taatagcgga tattggttcg ttcaatgacg acaacgcgct
    22021 tgtcttcgcg aagtttccac gccagctgac cataagcgac atccagcgcg taaactttcc
    22081 gggcgccggc ctgcaacaga caatccgtga acccgccggt ggaagcgccg acatcaaagg
    22141 cgaccaggcc ctcaacgttc atatcaaatt ccttgagcgc tccggccagt ttcaatccac
    22201 cgcggctcac atagggatta tcttccccct tgatatggat ttcggtatgg ggaacgaaca
    22261 atgtcccggc cttgtccgga taggctccat ccactttcac agcgcgggct aaaatcaggg
    22321 cgcgtgcctt ttccagggta ggcgcgattc cgcgttcaac aagtagtttg tccaaacgaa
    22381 ttttctcggc agccatagag gattgttgat gtccttcaag tcaagaagtc gagcaggctc
    22441 aagacggtcg tcggaatgat tcagacgtca gaatttcgtt gatggcggcc ataatccctt
    22501 cttcatcgat tccataccgt cggcgttgct gcgcctgcgt tgcatgtctg acgaattcgt
    22561 ccggaatgcc cagacgtttt acccggatgc cggtgatgct tttttcagcc agcagttcca
    22621 gaacagcgct tccaaacccg ccctgcagtg tgttttcctc aaccgtgatg atggtcccga
    22681 cagcggccgc cgttttcaga atcagttctt catccagggg tttcacaaag cgggcgttga
    22741 tgacgctgat atcccgtccc tcttcggcga ggcggttcgc cgccgccaga gcaggcagca
    22801 ccgttgaacc gattgccatg acggccagat cggttcctcc cctgagcact tctccctttc
    22861 cgatgtcgag agccgtcaga acctcatcca ggggtctgcc cgctccgctc ccgcgcgggt
    22921 aacgcagaga aaccggctgt ccgcaatcga cggcggtttt gagcatatgc tggagttcgt
    22981 tttcatcttt gggggccatg accacgatgt ggggaatgga acgcagatag gagtaatcca
    23041 gaagcccctg atgcgtggcg ccgtcctcac cgaccagtcc gccgcgatcc acggcaaaga
    23101 taatgggaag tttttgcagg cacacatcgt ggacgatttg atcataggcg cgctgcagga
    23161 aagttgagta gatggccaca acgggaatga acccttccgt ggcgaggccg gcggcaaagg
    23221 tgaccgcatg ctgttccgca atgcccacgt cataaaaacg attcgggaat tcctgactga
    23281 agagattgag gcccgtgcct tcgcgcatgg cggcggtaat cgccacaatc cgggaatcgg
    23341 tgcgcgcgag cttcaccagc gtcctgccga atacgtttgt gtaagtgggg atgccggacg
    23401 aggaagcaat gacctggcct gtttgcacat caaaaggggc gacgccgtga taactcggcg
    23461 gattctgctc ggcaaaactg tagcccttgc cttttttggt aatgacgtgg acaagcacgg
    23521 gaccggtcat ctcccggatg ttttcgaacg ttttaatcag atgatcgagg cgatgtccct
    23581 ccagcggtcc cacgtaggta aaacccattt cttcaaagag cgctccgggc accatcaggg
    23641 ttttcagaat ttcttcgatt ttctgggcga atttgatcat atctccgccc acgccgggaa
    23701 tgcttttgag aaatcttttg atatccgctt tcagggtggt gatcttatga ccggtcatca
    23761 cccggttcag ataggaggac atggcgccga cgttttccga gatggacatt tcattgtcgt
    23821 tgagcacaat gatcatgttt ttcttgcggt ctcccaccca gttcaggccc tcaaaggcca
    23881 tgcctgaggt cattgatccg tcgccgatca cagcaatgat tttatagctg gcgcccttca
    23941 ggcaggcggc ttcggtcatg cccgcggccg cggaaatgga ggttccgctg tggccgacat
    24001 taaacacatc gtaggggctt tcctcccgcc ggggaaaccc gctgatgccg ttttgtttgc
    24061 gcaggttttt aaaatcctct ttccgtccgg tgatgatttt atgcgcgtac gactgatggc
    24121 cgacatccca gataatttta tcctgcgctg tgttgaaagc gtagtgaaga gcgagggtca
    24181 gttcgacggt tcccagcgac gcggccagat ggccgccgca cgatgcaacc gtgagggtaa
    24241 tcaaatccct gatttcctga gccagcgtat tgagctgggc aagggtcagt ccgcgaatat
    24301 cggccgggaa attgacatcc ttcagaacac tgtaatgaac ggtttgcggt tgcttgacca
    24361 aaaaaaatcc tttcgttttt tatgacttcc tgtccctgat gtaacgggcg atgacaatca
    24421 gcggcatggc tctttcatca aaatcggcca gactggtgac ggccgcttcc acgtgctggt
    24481 tcagcttttc ttttgcctgt tgcagtccca tcagcgaagg ataggtcatc ttgttgcggg
    24541 cggcatcgct gccggttccc ttgcccatca accggctgtc tccttcaaca ttcagaatgt
    24601 catctgctat ctgaaaagcc agtcccacat gcatgccgta ttcggtcagc gcttgaattt
    24661 tatctttgcc tgcattgaac atcaccgcgc cggattttac ggcggcgacg atcaatgcgc
    24721 cggttttgcg ccggtgaatg tcctgcagaa ttgtttcatc cgctccggtt ttttccgata
    24781 tcacgtcaag cgcctgaccg cccaccatgc cgttgatccc ggcggcattt gcaatttcct
    24841 gaataatggc cagtcgccgt tccgcggaga gcatgacctt ttccgcacgg gacaaaagca
    24901 cgaacgcctc cgtgagcagt gcatccccgg ccagaatcgc catgttttcc ccgaaaactt
    24961 tatggcaggt gggtttcccg cgccggaaat cgtcgttatc catcgacggc aaatcgtcgt
    25021 gaatcagcga gtaggagtgg attaattcca gggcgcacgc aacggggatg gccgggacca
    25081 gatcgccgcc gacggcttcc gcggccgcaa gacagagaat cggacggatg cgtttcccgc
    25141 cgttgaaaac actgtaatgc acagccttgt aaatgtcgga aggaaatccg tcctcgggaa
    25201 gataacgctg cagagcttct tcaacaatgt tctgtctgtc ctccagatac gccttgagga
    25261 acagatcatc atcctgcatc atggtcgtct ccctgaaaac gttttacctg caaggtgttt
    25321 tccttctcca gaagcagttc aacgcggcgt tccgtttctt caagcttggc ggaacagaag
    25381 cgaatcagct tgatgccttc ttcaaaggac ttcaaggtct catccagagg catatcgccc
    25441 gcctccattt tcttgacaat atcttccagt ttttccagtg cgtcttcaaa tttttctttt
    25501 gccatcattg ctcctgaccc cggtaaacgg gagacgttct ttcatgaatt cgttccactg
    25561 cctcaaaccc gttcaaactt tttgacggat tcaaggatat gctgtctaac agaaaacaca
    25621 tctcgactca ctaaaatatt ttttgtattt tagcgttaaa attgcctttt gccaacagga
    25681 cattgacctc atcaccttca tgcaggtccc gggtctgccg gatgagcgtg ccttcgggga
    25741 ccgtgcgcgt gaggctgtag cctctttcca gaaccgcaag agggctcaac gattccagga
    25801 ccgcagcatc cttttgaaat cgttccttca atgccgtcag aacacaggac aggctgctgt
    25861 gaagttcttt ccggagattt tttaatacga tttgtttttc gttgatctgc tgctccggac
    25921 tctggtgttt cagacgcagc tccaggtggt tcagcttatg gagcaggatt tgccggcgat
    25981 gaccgaaagc ggcggaaagg ctctctttga gatcgtcaat tctgattttc tgagtgacga
    26041 ccgccaggcg cgggtcttta aatctttcct gcaacccctc cagatgttct ttttcagcct
    26101 tcagatgacg caagcagccg gcagtcattc tacgggccag catattaacc ttaaatatca
    26161 gttccgttcg gtccggcagg acgagctccg cggcagccga gggcgtgggt gcgcgcaaat
    26221 cggcagcgaa atcgcaaatg gtaaaatcgg tttcatgccc cacggccgat acaacgggaa
    26281 tgtccgaacg gaaaatttcg cgagccagcc tttcatcatt gaaagaagcc agatcttcca
    26341 gcgatccgcc gccccgcgca aggataatga cgtccacctg cccgaaggcg tggaaacggc
    26401 gcaacgcctg aatcatgtcg tttgccgcgt cattgccctg gacgcgcacc ggggcaatta
    26461 aaatgtccgt tgcgggaaac cgccgccggc tgacattcag aatatctctg atcaccgcgc
    26521 cggtgggaga ggtgatgacg ccgattcttt ccggcagaaa agggagagct tttttatatt
    26581 gtttgtcaaa taagccctct gcggaaagtt tggctttgag ctgttcgaaa gccttttgca
    26641 gggcgccgac accgagtggc tccaccgatt ccacaacaag ctggtattcg ccgcgcggcg
    26701 gataaagagt caggcgcgcc cggcataaaa tcttcagccc ttcctccagt tcaaaattga
    26761 cacggccccc ggttcttccg aacggacgaa aaaaaaccgc ccggatctga cttttgtcgt
    26821 ctttgagtgt gaagtaggta tgaccggatt gcggtcgccg caaatcggct acttcacctt
    26881 caaccagaag ggagtcaaac gattcttcca gaagagattt aatggtatta tttaattctg
    26941 aaacggtcag aatttgttcc atatggctaa tcctgaagct gaattatcag atattggaag
    27001 gtaaaacaag gttgaattaa aaggcctggt tctgtaataa acaaaatttc aaaccagcga
    27061 aggctctgca caaaattgga tgatgtccat gattttattg acaggcaaaa aacttcctta
    27121 tatcgtttac gcatggaaag aaaaatgttt tggaaaaaga taaagaacgc agtttgtccg
    27181 gtaaagcata ttctgcgtgt gcccgtgggg ctttttattg ttgcattggt ttgcgcggat
    27241 gccggtgcgg cccaaaataa tgaccggttg attcaggcag cggaaaaagg tcttttgcag
    27301 gacgttcaga cggcgcttgc ccagggtgcc gatacgaacg ccaaatcggt taaaaagtat
    27361 tataacattt tcggcaagga gataacgccg ttgattgccg cgtcggaaaa cggccatgcg
    27421 gaaatcgtca aactgcttct gcagaaaggc gccgatccca acttccagag gcttccggaa
    27481 agaaccaccg ccctgatgct tgccgcggaa aacggtcatg cggatgtcat gaaaatactg
    27541 ctggaaggca aagcggatat taatgcaata aacaacaccg ggataacggc actgatgctg
    27601 gcctcgaaaa acggccatcc ggatgcggtc ggtattctga tgcaggggaa agtcgatgtc
    27661 aacacccgaa acaatgacgg cgtaacggcg ctgatgctgg cagcgcagta cggtcatcac
    27721 gacatcgtcc agcgtcttgt gcagcaccaa gcggacgtca agatcaaaaa caacgacggc
    27781 gtgacggcgt tgatgctggc gtcgaaaaac ggtcacgcga aggttgttga gcgtcttttg
    27841 gaaacagatg ccggcgtgaa tgttcagaga gcttccgaca atgcgacggc gctgatgatg
    27901 gccgccgaaa acaatcacag ggaaatcgtc ggactccttc tgaataaagg ggccgatgtc
    27961 aatttgaaaa caaaattcat cggtgcgacc gccctgattt tgacggcggc aaaaggccat
    28021 gcggatgtcg cccgtctgct gatcgataaa ggggcggata tgaatgcgaa gaacaaagac
    28081 ggcattactg tgctgatggt ggcgtccctg aacggccacc gggccgttgt gcaactgctg
    28141 ctggataaag gagttcccgt gaatggcaaa agtaatgaaa acataacggc tctgctgatg
    28201 gcgtcggaaa gaggacacaa ggacattatt cagattcttc tggacaaggg ggcggatgta
    28261 aatgcaaaag acagcgatgg tttgcccccc gtgcttcttg ccgcacagaa aggccataag
    28321 gacgtggtga ctcttctgct ggataaagga gcggatggca acgtcagagc gaagatcaaa
    28381 gatacggaat atacgttact gacggctgcg gagatcaata aatggaccga cattgtggaa
    28441 aggcttaaga aatccggggt gaaagaataa tgcctgcttc cgtctgctga aaactccgag
    28501 cagaggcaac tgcacctgcc ttgcggccgt tgcaaacttt tttccaaaat cagtcaatac
    28561 ggaccggccg gggccgtcgg aatcgtttac cagataaaaa gccgccgtga aaagaagaag
    28621 gttttttcga cgacaaccag cggcgccgtt gatgcaaagc ggctcatcgc atgcagtctg
    28681 tgcacaaact ccgttgtgcc gcccgatgtg gttttttacg ttattttcct ttttgcggtt
    28741 cgaactgatt tttcgccgac cagaaagacg gcagatgacg tattaagtac aaaaggctat
    28801 tgcgatgctg ccagatgcga ttgcggaatt ttttacgcca ttttttatca gaataaaaac
    28861 gcttcacgta ttcgttatag agctgatcca gccgttcctt cgacgcaatg cccctgggta
    28921 caaaaacaaa attcagacag ttcatcagcc gccagtcttc gtgaaaagtc ccctcctctc
    28981 tgatggccgt ccacagcggg gcgccgggaa agggcgtaaa cttggccatg ttcatatcgt
    29041 cgaggtccag cgacagaata aaatccgatg tgcgtttgac ggatgcctcc gtttcgccgg
    29101 gcagccccat cataaaaaga cccttggcgc gcaggccggc tttttgaatt cgttttactg
    29161 tgtcgcgcac gtcatccagg ttgatgcccg atttgtgccg ggcaagcatg gcggggtcgg
    29221 ccgattcgat tcccagcgac accatcaggc atccggcgtc tttgagcatg tcaagcaaat
    29281 catcatccgt gtaaccgatg cgtacggcgc agttgaaatg cagacccaga ggacggcggc
    29341 tgagtttttc gcagagcttg acaatccgcg gacggttggc ggtgaataaa tcgtcgtaaa
    29401 tgttgatgtg ccgtacaccg aatgtctccc gcagatattt catgtggtcg taaatgtagc
    29461 cggcggaatt gtagcggaag ccttttttaa aaacggagcg gtcgcagtag gagcactgat
    29521 acagacaacc ccgcgacgta atcatggtcg cgcccggcgt atggatgtag ctgaacaggg
    29581 gcaggtggta atcgcggggg aaacctttga gctgttcata agcgggaaag ggcagactgt
    29641 ccagatcgga tatcttcggg cgcacatcgt tgaccgtgat ttcgtcctgg ttgcgccaga
    29701 ttaagccccg gatgtcggcg gcgggaatct cgtccgcgag ttcggcaatg gtctcctcgc
    29761 cttctccggc aatgagaaaa tcaaatgccg ggtaagcgtt cagaagtttt ccttccaaag
    29821 cggatacgtg aacgccgccg cagacggtgg cgatggctga agaatgattt ttgatgagcc
    29881 gggccagatc agcggcatcg gggaatgagg atgttgtggc cgaaaagcca accatctgcg
    29941 gctgataggc cagaatggcg ccggcctgtt tttgcagatc gagcggcgct cccggtccca
    30001 gacagtcgta gacgaaaacc tcatgtcctt tttgtttcag ataggcggcg atggacagca
    30061 cgccctgcgg caccatccga ttggcgatat cggtgatatc gcgtttgccg ggaaaccagt
    30121 tgaatccccg gggatggaca agaacaatgc gttttttctg ccgggaagaa accatcatga
    30181 ttttgttgag cctgaaacaa aagcatgcct gcctgaggca ctgcagttat aatgcacaaa
    30241 aacggaattg gttttttcgc gcagcgttcg cgcgatgccg aaagcggatg ccgtggaatg
    30301 tattccgcag cagggcagat aatcttccgt ttcccagccg ccgtctttcc agccgttcag
    30361 gtccgtttgc ctgatgcgga atccgggaag aacaatccgg ccttttcctc ttgcatcaca
    30421 aagatctgac agaatcgttt gcttatcgaa acgttggtcc ggcgcgccga cagtgtcgta
    30481 aaaatccacg gctagaatat gccctatatt ttgttcttca ttcttgccca gcaggagcca
    30541 ggaactactt tcgcccatcg gcagtctgcg ggcattaccg cagctgacat tcacccgttc
    30601 gcggtattct tcagccgtgg gtgtaaagat atcgcagccg ccgaccagcg cgatgtctat
    30661 ccggccgtgt tccagatagg tttgtgccag ccagagcgcc gactcgaatg ataattcttc
    30721 ctgtgagacg gtcagaacgg ttccggtaac ctcgcaaatc cgcgccatca cgaaagcggc
    30781 ggcattgctg accgaattgg caaactggtt gggactggga aacccgccgc catgtttgaa
    30841 cacctgtgtg atgaaaggca ggatttcgtc aacattgccc agaccggtgg ccagaaagat
    30901 tcccgttttc ttccccttca gataatcggg tgagacctgc tggaggcagg ccaggccgcc
    30961 gcaggaaacc atcttgatga atcttcccgc gcggcgcatc ggctgtccca taacctcgtt
    31021 gaccagagca tcggcattca agggcaattc gttctcttcc cagagcgcat cgcggttggg
    31081 catcagcggg ggaccgatat aacttgccgc gatcaccggt atgggtgagg ccggggccgt
    31141 gtgtttggtc gtcgtttctg tcatatgcat tcccttatcc gatagggttg tatctttcag
    31201 gacctgttat gatgagccgg cacgagcgga ggagttttcg gtggagttcc tgataatcaa
    31261 ataacttgtg catgttccgc cgaagccgaa aaacgtaaac aggtaagcgc cgtcgcgggc
    31321 gtcaatattt ttttgcgaag gggtaaagcc gatttccgga tccatttcac tgaaaccgaa
    31381 actccgcggg atgattccct cgtccaggca ggaaagccag agagccgttt ccgccgtgcc
    31441 ggccgcgccc agcgtgtggc cgatggcgcc cttaagggaa accaccggag gaagtttttc
    31501 cccgaagagt ctgaccagtc cccggccttc ggaaagatca ttgtcttttg tacctgtgcc
    31561 gtgcgctttg atggcgatga tatcgtcggc cgatagcctg gccgagtcca gggtatggcg
    31621 cacgacctcg cttacggtca cgccgtcgat gcccgccgat gtcatacttt tgatatcgtt
    31681 gtagagatac ccgccgctga atgtatagcg ggaggataaa ttttgaccgg aatccagaat
    31741 gaccgcggac gcggcttcgc cgatctgcat gccggcgcgg gtggcgcaga aaggccggca
    31801 tcgctcgtaa tcaaagagca gcaggctgga aaaaccgtgg agcgtcatat tcagaagcgg
    31861 ttcaaaaccg atgacgatgg cccggcggat tttcctgctc tgaagaagat cggcggctac
    31921 taccagcgca tgcgaactgg aaacgcaggc gatgctgaac gtcagcgcca ttccccggat
    31981 accgtatttt ttggtgatga aatcggccac ttccccggca ccgcggttgc ggcaggaaat
    32041 cggcggcgtt tcgccgtccg gatttttgcg gacggcttcg ataaattcat gttcgttgcg
    32101 gataaaaagg ccgccggtcg tgccgatcag gacgctggat tcggcgattt catccgggga
    32161 taggccggcg cgctcaaacg cctcatcgat cgcgccttcc agcatggcca tggctttttc
    32221 cagagggttg cccggatggg acacttcgaa aacattgaag tcgcggttaa tcagatggga
    32281 agaccgcccc ggttttgccg gtggggcctg agcctcaatc aggttgcgtg ccgcatcggt
    32341 ggcattccat cccagggtgg ttaccgcgcc ccagccgcgg acataaatat cgccgctgtt
    32401 cattttttaa cccttggagc ctcgatgata aaatcggcca gcgtgtcgaa attctgcatt
    32461 gctttggcgg ccgttgaagc gttttttaat tcgacaccga actgaaatct gatttccatg
    32521 gcgatttcca tggcgtcgag actgtccagc agcagcggtc cgggaccgcc gatgaaggga
    32581 acatcggtgg gcacgtcttc cgaccgcaca tccgtgattt cacaagcccg gatgaccagc
    32641 tccttgagct tgtaaatcaa ctcttctctt gatgacacgt ttaagtcttt caatttctgg
    32701 gttacatcca tttaactttc ctccccgcta tataaaaaca aaccaacgcg aataccgtta
    32761 aaacagcaag cttcggccat atggaaccga acccggcgtc gcgcgaaaaa atatccaaat
    32821 aagcctgatg cgcccagtac atcggtgaga gcatcgccag ttttttcatg atgagcggca
    32881 tgaccgcgtg cgggaccata ataccgccga agacggccat cagtaccgct ccggtggccg
    32941 ccagcgccga agattgttcc ggcgcccgtg ccagcgccgc gaccatgacg ccgaacccgg
    33001 tagatgcggc cgcgacgacc agcgtgaccg gtatcacatg ccagggatgc tctcccagcg
    33061 taaaagggaa tgcaatgatc agcggcaaaa tataaatacc gacgatcagc attaaagcaa
    33121 attgcatcag attaatcaaa taataaggaa tcagcttgcc gagaatgaca atagcggggc
    33181 tgaccggata ggtaaaaatc cgctgcagcg tgccgtcgtt tttttcccgc agaaaaccga
    33241 tcgacatggg aatcgctatg aaaaacatcg caaaaatcga ccagccggga acgctgtttt
    33301 gcagcgggcc gggcaaggtg cggttcgttt ttgtcttttc aacgataaaa tcggccgaca
    33361 tcccttcgat gtcgtcgatg cccatgacca cctccacggt caggcccgtg accagaacac
    33421 gcacggcgga tttgtatccg gcgtcgagaa cgggatcgaa ctccatttcc accggtttca
    33481 atccttcatc aaatcccttc ggtatgaaaa tcaccgcatg cgcccggctg gtttcgaaaa
    33541 ccttgtcgtt gtcgaatccc ggcggtctct ggacgcgttg aatcattttg ttggcgctga
    33601 tccgttgttc aagcaaattc gccgtcggct gatgcgattc gttttcaata atcagcagaa
    33661 tctgccgtcc cgtcaattta tccatataaa cgccctgcag cgccagcgat agaaaaaaaa
    33721 tcagcgccat gggcatgatg aataaaagcg gaatggtctg gcgttcgcgc agcaaaatga
    33781 tcatttcctt gcggatcaac gctgtcagtt tccggaacgt cactttcgtt tcccctcttt
    33841 aatcctcatc gcgcaatctg tgtccggttt gtgccagaaa aaaagtttcc agatggtcga
    33901 tttcgttcat ggcgccggaa aaaatcaccg ctccccggtc cagcatggtc acctttgaac
    33961 acatccgctg cgcttcgtcc atgatatgcg tgcacagaac gatcgcaatt ccggaggcat
    34021 tcattttttt cagagtttca taaatatggt tgcggctctg cgggtccacg ccaacggtcg
    34081 gttcatccag gagcagcagg cgcggtgcgt ggagaagggc gacggccaga ttcagacggc
    34141 gcagcatccc gccggaatag gttgacgccc gtttctgcgc gtaaggcaaa aggccggttt
    34201 cttccagaac cgcccgggac cgttcttgca gcagctcgcg cgacaacccg gccatgacgc
    34261 cgaaaacgcg catgttttcc atggcggtca gtcccgcata tacggagatg ggctggggcg
    34321 cgtaaccgac ggaactcagc gattgtgcat gatcgttgcg ggtgccgtaa atcgatatgc
    34381 tgccctgaaa tccacgcagc tgcccggtga tcaggcgcat caacgtggtc tttcccgctc
    34441 cattaggtcc caaaagtgcg tgcatctggc cgctgtccac cgacatggaa aaatcgcgca
    34501 gcgcatattc agccccggcg gttttgattt ttccctcttt ggggttatag gaaaaagtga
    34561 cttgcgcaat gtccagagct ttcatgattt cagttttccg gcaatctcat gcagattttt
    34621 aaaaagattt tcttcacggc cggcgcagat cagcattaat ccgcccgctt tggcaaacgt
    34681 ctcatcctgt tttcctcttt ttttgatgat gcgcgcggcg gtgacggcaa actcgcgcca
    34741 cgtatcgccg atggccgtca tgtcctgcga tagatcgttg agttccgccg agccgaaaag
    34801 gtccgcggct tcctgcagaa aggctgcgta catatagcgg aatccagcgc cgcccgtgcc
    34861 gacttcctcc tgcatgcgga cgatcatgcc caactgccgg caggcttctt cgaacccctg
    34921 cgtcttctcg aatttgatga ttcgtttcgc caggaagcgg atgccgcgga tgccgaaaat
    34981 cggtaccgga attctgagca tcatgttgca ggtatccagc atgcccttga tgcaggcgcc
    35041 gcgtaagtcg ggatgaagat taaccgagac cgggtaatac atgaacccgt gcggttccag
    35101 cggtccgcgg gcaaaccgcg cccggatcag gtcttcggtc cggcaatcca ccggtttatc
    35161 caaaacggga tcgctcacgc ggaagccgtt ttcaatttcg tgaagaataa cgatgttgtg
    35221 cccgttgaat tgaaaccgga aacgatcggg aaaataagaa agccagtaaa tattggtggt
    35281 cagtccgaca atctggccgc tgcgcagcac acggagcagt tcgtccatgc ctttttttgc
    35341 gttggtgtat ttttttgtga caaattttcc atcgagcctt ttggcggctt tgcgaaacac
    35401 cgcacccggt tgcgcccgat atgtgctgat cggcaggccg acaaatttaa caaacggcag
    35461 atggccgaaa aagatgccgc tgccgatgcc gaaaatcatc ggctcgctga tttccaatcc
    35521 cttatcccgg aatagcgccg ccgtcacgcc ggtttcacag tgggcggcat gatggtgaac
    35581 aaacggtttc ccgccggatg tcagttctgt atgagtcaat aataagtcct ttaatgagtc
    35641 ccaagttttc atgaaacgcc aattccgaaa gcgaataacc taaaggacgc tataggcgca
    35701 atggaattgg taattgtaat gagtttcaaa tttcaaaagc aaagcgtatt gaaatttgcc
    35761 agccgaatta tccaatcccc atgaatcggg attggaaatt atcccactaa cgacgcttag
    35821 aggcaccgtg ccgcatgcta caggatttat tcccgcgatt cctcaggtac ggtttttaat
    35881 tcttccatcg tgactgcaag agctttcgca tagcgttcca ataccggatt tttcagcctg
    35941 gaaaaaattt ccggccgcag atgccgcttg acgcgaaaca cggcaatgcc ggcggtcttg
    36001 cccaggatat ccggtatcat ctgccgccgt tccatgtgat aggcgagcgg gcttttttct
    36061 ccggccagta cggcttttct ggttttctcc agatttacag ccagttcctt tacagccaga
    36121 ccattgacaa gctcctctgc ttcccatccg ttactggaca caatgacata cctgcctttt
    36181 tcatcgaccg catagcaggc gcgcttcacg ccttcataat attcaaccgt gttatcctgc
    36241 ggcacttcgt tgactttcac gaataaatcc ttcttttcat ttacttatgt agtttcttat
    36301 actaaattta aaaaagcgaa aagagatttt taatgagacc cgttgataat gagccccaag
    36361 cttcagatac agagtaaact gaagctggct ggacgaatta taccggcgcg tagcgcagtg
    36421 gtagaacgag ctttgtgaag tttgcccctg ccaccctaag ggtggcggga taatttgctc
    36481 taagattttc cgggcactac gtttcggaaa atcaaggctc atgaaattat tcccgaagcg
    36541 aagcggagtg ggatttttcc aaaaacggct cgaaatgatg ccactcgaag ggatgctggc
    36601 gggcaaattc agcgagatca tcggcatagg cctgcaccga attctgcacg gcttttttcc
    36661 ggtcagcgcg tgcggcggca gtaacctttc gcccaggcga aatccggata tgatactttc
    36721 caacggcttc ctgataaaca aaaaacgtca tcagcggcgc tccggtcatc aatgcaaaca
    36781 gatgcggcgt atccggcagg cgaacctcgt gcccgagaaa attcaccgcg atatgactct
    36841 gatcgcccca taaacggtcg ccggccatgg agacgatgcc gccttcccgc aaaaaattga
    36901 tgccgtcgat cagggcgaac ggtgatttct cgtcttcgga tgttgccacg atttttatgc
    36961 cgcttttggc catggcttct ttctgaatac gctcaatctg ttctttgtgc ttcgcgccca
    37021 gatacagcat gatcggcagg cctcttttgt tgagcgtctg cgcggccagc tcccagttgc
    37081 cgatatgcga cattagcagg atcgcacccg ttttgttctg cacggccgca tcaagatgtt
    37141 cccatccttc tttgatatac tcgatatcgt tttcgtcaaa gcggatgaaa cggtggatat
    37201 agacgtcggt gaatttatga tactgttgcc atgcgcacca gagatggtag aaaaagccct
    37261 tccccggaaa taaggtgcga taaaactcga aactgttagc cacacgcacc ggaaaaagaa
    37321 aaaaataacc cgtggcgata aaccaggaaa tgattcggaa aaaccacaac cccagcctgt
    37381 gggtgaaaaa gagcaatagc ttataaggta gtttcttcat aactcaatat cttgtcatct
    37441 tcgacctgat cggggacccg gtatccagtc ctttttcttc caacgtgagc caggtaaaga
    37501 tttcgaccct tattattttt taatgatgga agccgatcca gtttccatcc catcttgcat
    37561 tgaggaccag gggatcgtcg gatttatacc agtactcttc gatgaagtgg tcgaatgcaa
    37621 ccttttgaat gaaacatata tcggaattac tgctgtccac cgctacgcaa tcacattgat
    37681 ttcctttgtg attttgatcg tttggatcca taaaatacac cgccttctcg gcatcatcat
    37741 atccgatcac cagaacataa tgaccggaag aaggaatgcc cagcaccggc gatctccaaa
    37801 ggtgcaccag cactggagag ccgctattca aaaacggaac cattcgatta agaaaaatat
    37861 tccttttttg ttcggaaatg ctgtcctctg ttccggtcag atcattgctg taattttcta
    37921 tcatcacctc actgtacaac gcccggctac ctgattcatc cagaaggttt tctgccgccc
    37981 gagctaagga agtcaggtgg atgcctgcca ggttgttgaa tgagttgtcc atgtattgaa
    38041 tatatgttgc tatttgcgat gttgctgaca gttccgtcgg ataagcaatt ctatctgtga
    38101 tttccgccgg acaattgtcg tcatcaattc cggttttgat atttttcaga tgatcgccgt
    38161 aatatttcat ggtcatgtaa aaacatgttg ccgcgcataa tcccgcttcc tgcgagacgt
    38221 agtagtatga cggattgttc cggatcgtct catagggatg cggtccggca atgttgtccg
    38281 cgacaacagc cagagaaaga tcctgtgtgc tggagcctgc ggcgtcttcg accgtcacgg
    38341 taaattgaaa tacccctgga gctgcccatg tggtgccggc gattctgccg gaagaagaca
    38401 aagtgatgcc tgagggcaat gaccctttgg tgatggagaa cattttcgtg ccgttgccgc
    38461 cggttacgct gatctgctgg ctgtaagccg aagcaatgac gcaggagggt aatgaggttg
    38521 tggcaattct gggtgaagac tcagaatcgg agccggacga accggaacag ccgctgaaca
    38581 caacactcat caacaataag accaccccac aacatacgct caaacttttt ttcatttttt
    38641 atcctgtctt ctgatctctt tctgtaaact tttacggtaa tgcaagaaat gatatatgac
    38701 attgttgttt attcatagtc tctcttgctt tggctcggtc atgcagaggg tttgctgcgt
    38761 ggctaaccct tctttcccat aagcaaagcc tccttgtttc ttgtctcatc tgaccgcgta
    38821 aataatgaag accagtccta tcaaaatgcg gtagtatcca aaatatttga atccatagtt
    38881 ttcaacaatc tttaaaaata tcttaaccgc aagataagca aaaataaaag caaccgcaaa
    38941 accgatgact aacagcgtct gctcgtaaga cgaaaaggcg gcatcggttt tgatcaaatc
    39001 gtaccccgtt gctgcacaca tcgtgggaac cgccagcaga aaggaaaact cggtggcctg
    39061 cttcttgttt aatccgttga aaacgccgcc aataatggtt gccgcggcgc gcgatacacc
    39121 gggaatcatg gaaacacatt gtgccaatcc aatcaatatg gcgtgcttgt aggagatgtc
    39181 ctccaactcc atatattgtg ttttactgtt caccacgcgt ttgtctacga ggatgagaat
    39241 gacgccgccg acaatgagcg ctgtagaaac gatcaatggg ttgaacagat gcattttaat
    39301 ggttttatag gccagaaaac caatcaccgc cgttggaata aaagcaattc cgagcttgat
    39361 gtatattttt atgcctttca gaaaacgctt atagtaaagc atgatgatcg ctccgatcgc
    39421 tccgatttga atggcaattt caaagctctt tacgaaagca ttgtcgtgaa tattcatcaa
    39481 ggatgaggct aaaatcatgt gcccggtgga ggaaaccggc agaaactcgg taatcccctc
    39541 tatcgctgcc agaatgattg tctgaataat attcattttc cccctcaatt ttcgttacga
    39601 cacatagatg gaatcgtgcc gtattatttt ttataccggt aaaaatctac ttttattttc
    39661 gtattttcat aaaggatttt atatttacgt gttttcctaa accgtttttc tgcatttttc
    39721 aaacatttgc gggaatttcc tgccggccta atatttttta cccgcaaacc atttttcttc
    39781 aatactccgg gtatcggaag cgaagacctg gacggcaaag ccggcctgtt tcatgaaatc
    39841 ggcaatttcc gtttctgatc gaaaccggtt gggcatgccg gtcattttta atcgactggt
    39901 ttcgatccag cgtttccacg gattgggttt atccgacgga atggttgtgc ggataagcaa
    39961 tgtcccgtct gcagtcagtt tatcgtaaat ccgctgaaaa acaattttta cttcatcatc
    40021 ggaaatcagg tgcagcatgt cgagcatgag aacgtgatcc gcctcgcctt cgacatgcgg
    40081 taaatccggc gcccgtcctg cttccaccct gccacggctg ccgatgacgc gcgaggcgat
    40141 caaaacccgc tcaacatcgg gttcaatacc gaacacccgg gcatccggat aaatttccag
    40201 aagccaggtg gcgggcacgc caaagccaca gccgatatct ataatcctac ggggattttt
    40261 tacatactta tgcagttccc tgaacatcgg atccatcatt attttcatgc gagcaaagac
    40321 cctcggatac ccgggtagat gccggtagcg caaaacggtc cgccgcagat ggtcgcggga
    40381 accgattacc actttgtccg cggggtatgc aatcggcgca aatattttgt ccatcagaga
    40441 cggcaggata aaaaagacgc cgatcaggga gtaaatgatt ccgagaaagg agacaatgcc
    40501 gatactgcgc aaaagcgcgt ggtcggccag tgccagcacg ccgaagccga ttaaggtggt
    40561 cagcgccgcc agaaatatgg ccagtttgat ggtgtgcatg gccggacttt tttcatcgac
    40621 gttacgctgg taatgacaaa tgtaatagac ggaataatcg tcgcccatgc ccatgatgat
    40681 gatccagagc ataattccgg gaatatccag cggatggccg atgattttca tcgtgccgag
    40741 cgtggcaaaa agcgcgaaga caatcggcgc cattgctgca agcgtcagct gccagtccag
    40801 aaaatataga aaagtaatca acaccaggcc gatgctgatg atgatggcga tttccaggaa
    40861 aagatctttc agaaaatcgc ccagccgctt gttgaacaag tcagcgtcga atattttagc
    40921 cagaccgctt cgggaaagcc gctcaaagaa gtcttccgcg ttgtaatttt taccggaggc
    40981 gagcaaactg agttgcgtat aacccgcgga acttttggca atccccagca tatcgaaata
    41041 tttttcaggt atatcgaacg agccgatggt tgtccgattg atgattttcc agaagggttc
    41101 aaaagcatcg ggcgcaaaat cgttttcctt ccccgctgcg cttagattgc gctttaattc
    41161 agccacgcgg tctttattcc agaaggcact ccaggccgca taattgtgtt gggccgtctc
    41221 cggggaggga aacaaaacgg tcggcagaaa aggaggagat aatttttcat cccggatatc
    41281 cccagccagc caaccggcca gttgatcgtt tttttcccga agttgcgtca tatcggatgc
    41341 ctgaagaaat acataacacc ggccggaaag attaccccac acctcctgca tttttttgtc
    41401 ggcattcatg gttgcttcgc tcatggagtt catcgaattc atatccacat tgaaaaccgg
    41461 cttggcaaag aaaagcatcg ctagaccgaa aataatcgcg gcaatcaatt tccatttggc
    41521 cggagcggca aatttttgaa cagcattaga caacagcggg ttgcccggct ttctcgccgg
    41581 cggcatggcc ggaaatattc ttggaaaaac aagatggaca aacagcagag cgaaagcgac
    41641 gcccaaggcg gaaaaaacgc cgatttgcgc cagaatctta aaatcgctga tgagcagcag
    41701 cagaaaagag ccgaccgtgg tcaggacggc cagaaactcc gccgaccaga cttcccgcgc
    41761 cacatctttt ccgcaggtgg catggggctg atccagaaaa agcaggtagg cgatgcccag
    41821 atcaacggta aaggccataa tggcgctgcc gaaaccgaca gcgataatcg acatggattt
    41881 gaataaaaaa gaacaaacga agagagcggc aatcgcgccg acggacgaag gcagcagcgc
    41941 cagcaggccg atgagcggac gcggaaacgc aaggatgagc aaaaaagcga tgcccagggt
    42001 tgtgaaggtt acggccatct gcatatcgct tttggctgtg gtttcgttat ccagcgccac
    42061 acggtaagcg cccgtggcag tcagtttata tggatggcct tttgcggcta gatctctccg
    42121 gcaatcttcc agaagttttt cgattttttc agccactgcc gtgtctgttc ctgaaccttt
    42181 aattctggca acgatcaacg cgtttgtgcc gtcttcggaa atcaattgcc cctgataaaa
    42241 cagcgccttg ttggcaggca gcagggcgga catctgtttg agaatgacac tggaaaatcc
    42301 aagcgggtct ttggtcatca tgtcgccgcg gccaattcct tcaagctgcc caagggcttg
    42361 ccggttttgc gccatggcct ctctgatgtt atccggcgtc agcagcggcg caattttctg
    42421 ttccagatca tcagcactta aaagtgcggg caggttatcg ttgacatgag cgataagttc
    42481 gggaaagttt tcagcgtcgt ccccgatgcc cacccgaata aacaggccgc tcttgttgag
    42541 ctggtcggtg accgcggccg cagtgtcaat cagcttgttg cggtcgggag atgtctgttc
    42601 aagattaatg aacaccttat cctgaacggg caggtgtttg ataatcagac gcgcgtctgc
    42661 caataccggg tctttatgcg gcatggattc cagaatatcc gtatcgattt taagatttct
    42721 ggattcccag acagaaagcc ctgtgacaat gagcgcaacg acaaaaataa tggaccagtt
    42781 tacctgatat ttgcggatga gcggcatgaa cacctttatt tttcatttag ttatagatac
    42841 ttaaggctga ttgtgtaaat catattttgc cggtcgtggc aagataaaaa ggcggcgtcg
    42901 taatcttgca gcgccgtacg ggtcacgttt ccacgcctga taaggttatg tcaagggagc
    42961 atcgtttatt taagaaaaaa attttgaaaa atcagatgcg tggagattga aaaatgtacg
    43021 gctatttgta aatgaatata taattcaggc agaataaagc ccaacctatt atattctatt
    43081 gagcaatgct tttcagcacc cggggcaatc cattgccaag cccaaaacga cttattggtc
    43141 tgatcccatc tccattggat ttaaaataat gcagcatttt tgtatgtttt ctcatacctt
    43201 tcatgacgcc gacaaagccg caaagcagaa aaccgttttc cgttctttta atattggatt
    43261 cgcttatccc aaacgtccga ggatgcacaa attgaatgaa aagcctgatg actttctttc
    43321 ccgcccttcc caccgggtaa acggtatttt tctcaaaatc gccatacggc tcattaaaat
    43381 aatccttatt tcgggaagaa taacgtattg acaggtgctc gcccggatac actatctgat
    43441 gttctttttc ctgaacgtgc ctccaaaacc accatctgac cattttcctt tttacatcgg
    43501 gccttccggt ttccatcgcc acgagatatc ctccattctt cagtttgaca aaaccaatct
    43561 catgctgcag cgtttctttc tcaaatattc gattgacgtc ttgatattcc actttgctga
    43621 tttgcggccg gtatatgtgc atgtcctcta tgcccgtgtc aagatcaggg tttttcattt
    43681 tctcgcagta agaataatct tcttttttca tgatgcttgc ttttgccgta tggagtttgt
    43741 gttgacaatc aaaaagttta ggtcacgctc tgacacaaat caagaacgat gagcaatgag
    43801 acttttgaaa agaaattatt caggatgacg atggacgttt ttggatgtta tcgtggcgga
    43861 aatatccggg tgaaaatcag acgggtgaac gttgaggaat ttcggcagaa atccacgaac
    43921 gggcggaaat gagaaattct tcccccttcc ggattatagc ggacacggat gggcgcttca
    43981 ataactggga tgccgtttct ttcggcgtgc accagaattt ccacttcaaa ttggaagcgc
    44041 cgtgcctttg ttttcagatt gagcgcttcg ggcaaaggat aaatgcgaaa tccgctctgg
    44101 gaatcggaaa tggccggtcc accggaggcc cgcacccaga aattggaaaa cttttttcca
    44161 aaactgcttg tccagggcac atgtttgccg gccatgcctt ctctggctcc gacaacgatc
    44221 ggccgtgtat ttttgggaat agccttgata agcttttttg catcttcagg atagtgctgc
    44281 ccatcggcat cgactatgat cgcccaatca gccattttcg cagctgcagt gaagcctgta
    44341 aggatggccg cgcccttccc ttgattttgt tcatgttgca ggagttgaat gtctttgatt
    44401 tcttttatct gattatacgt gtcgtcagtt gagccgtcat caactacaac aacaggatag
    44461 ccaagttttt gagcatcctt agccacctgc gctaccgttc cggcatgatt ataaacggga
    44521 atgacaaaag caaaacggtt tttgttataa tcttttgacc tggtcaatgt ttgtttctca
    44581 ttttaacgga tgttgatttg agggagaaac gccacccccc acgttcccgg ccccatgtgc
    44641 gcgccggacg tcagcgacag cggctgaagt ataatttcag catcaggata cagttccttt
    44701 atattttttc gaaccgtgcc gtcgacaaat cgtctgttgt tggaatattc cagcatgatc
    44761 agcgcacggg aatcgtcttt gatcgttgcg tcaagtttag ccaaggcaaa cgtgatctga
    44821 tcctcctgat ttttggccgt gccgactttt tttgcgccgt ccggcagcgg agatatcacc
    44881 ggctttacgt tgaatttgtc gccgaaaaag gcgctggttt tggaaaggcg tccccccgcc
    44941 gccagataat gcaacttgtc cagaaacaga tattcttcgc acaggcgtat ggccttacgg
    45001 gcaaaaataa tggtgtttgt cacgtcacgg ctttcggcca gataccgggc ggtggacaaa
    45061 gcgatggttc ccagtcggcc cgatgccgct cccgtatcca agatgctgaa ccggtcttct
    45121 ttgtcgtgat cttttttcca ctggacggcc gcctgataat tgccggtaaa tacggagccg
    45181 acgcaaaggt acaacacttt atcaaaacgc tccagcgcgc tttgataaaa ctgatgccgt
    45241 tcataaacag aggcctggga cgtggatacc cggatgtcct tcttcatggc gcggtagagg
    45301 tcttcaggat gaaaaaaggt ttccggcagg cattgatcgc cgatagtcag ataactgttt
    45361 aacagggtaa aaccatggat cttggcgtcc tgagtcgtca gggaaccggc cgcatcggtt
    45421 ataatatgca atgtcttttc tgcatttaaa tgcctgaaat ccgctgtttg acaactcagg
    45481 tcgtcatctt cccaggtcac caaagagccg agccctgtta tttgagagcg cacctgctcc
    45541 ttgtcctgcg tgtgaagatg aaccttgtaa aactcccctt cgggaatgac gatcacttct
    45601 tcctgcacgc tggtgatcat ttttatttca tcgggagaat agcggggcgc cttgaccaca
    45661 aaatcaacgc agtaaccgga ttctatgttt tcctgaaaag acgatgagat gtgcagccga
    45721 tcatggaata tttccgtcag aggacgacca tcttcggaaa ggcctgtgag tgtgtaaaag
    45781 aatccttcaa aaaaaatata gattcccagc gcgcccgcat cgacaacacc ggcctcctgc
    45841 aatttaggca gttgctcata ggtttgatgg accgcgtttt ccatgcttcg gatgatgttt
    45901 tcaatgtttt ctttatccgg tgtgaattgt tccgacagta cgtcggccag ggcgtcaaaa
    45961 acattcagca tggtcccccg acgcggattg accagggcct gccaggcctt gatctttcct
    46021 tcacgggcac gtccggccag gtcggcgatg ttttccaccg cgcagaaggc ggagaaaaac
    46081 tgtgaggcga tgttaccgga attgccgcgc gccgacagaa gcagccgatg aatcatcgtt
    46141 gcttgcggca gatcggtgtt gcgaaggggc atcagactaa gggaaagatt tctgccggtg
    46201 tcgccgtcgg caaccggaaa aacattgatg gaatccagca gatcggagcg ggccataaga
    46261 cgttccacac cggccgtgaa ggattgtttg agcacctcat tcatttcaga aaccaaatgc
    46321 ccggctgatt tcttcgggaa tgcgaaacag catttctctt tccggaactt ttacggtgac
    46381 ctgttccgtg cgtccgcggg caacaatctt cccgtcctcc gcttttcgga tttcaaaatc
    46441 gacgatcagt ttaatgcggg cttcaaccag cgtcgctttg cagataattt catcgcggtg
    46501 aaaaagcggc aaaacgtgtt tggtcgaggt tttgatgatc ggaaaaatga gctgatgctt
    46561 ttcataaaac gaataaagat cgatgccgcg gctttcgaaa agcgccgcac gggcgatttc
    46621 aaaatagttc agatagttgg catgccagac gatctgcatc ggatcgagat caaaaaaagg
    46681 aactctgatt tttacgtcga tactgttttg tttttctttc atggttttat tttaaaaaag
    46741 tccgaatatc gaatggcggt gcagatattt tcaatatctt tatccattgc cctgtcttca
    46801 atgaccggcg cgctgacgga tctgatcttc cccatggttt tcgacagcag cggtcttgcg
    46861 ttggtgttgt cccggatgtc gcaggcctgg gccgccgcga gaagatgaat ggcaacaacg
    46921 cgttccgtca acgtgcagat tctttcggca tcgcgcgccg cgatggtgcc catgctgacc
    46981 ttgtcctgat tatgcgattc ggtggaccgg gaaaatgacg ctgccggcat ggttaatttc
    47041 agtgcttccg ccgtcagcgc ggatgatgaa atggacatgg ccttgaaccc gtgccgcaac
    47101 cccttttcgt gtggcggctt tctcaccagg tcgccgggca gccctctgtt cagattcgga
    47161 ctgaccagaa gcatgatctg gcggtcgcac atatcggcga cagaagccag cgccgctttt
    47221 agtccgtcca tggcgaaggc gatgtgcccg ccgtaaaaat tgccgcccat caggatttca
    47281 ccggtcgcgg gatcgaaaat ggggttatcg ttggagctgt tggcttcgat ttccacccat
    47341 gtcctgatcc attcgaggcc gtcatacagg acaccggcca cctgcggcgt gcagcgtaga
    47401 gagtaaggat cctgcaaggc atcgagggaa tcgtcttcca ggtgccgcgt cgaaattttt
    47461 gccttcaaca ggcgggtgat ctgagcggcc acaaatgcct gaccgggaaa aggctttgct
    47521 tccgaaatca cgggatgata atgccgggca ttgcctttga gcgcatgaat cgaaagagcc
    47581 gtggcgtaaa tcgcagccga taaaatcctg taggctcttt cgatcgtcaa agcggcgatg
    47641 cccgtcatgg tcgttgtgcc gttgagcatg gccagaggtt ctttgggaag atacgtgtag
    47701 ggctttagcc ccgccttttt catggctctg gctgcggaca tgacggtgcc ccggtaaatg
    47761 acgtcgcgtt cgcccatcag cgtggcggcg atataggaca tcggcgtcag atcgccggat
    47821 gcgccgaccg aaccttcaca ggggacgatc ggcgtgattc cgtgatttag aaaatcggcg
    47881 atctgctgaa gcagtgcgat cgacacgccg gaatagccgc gcgccagaca gatcatacgg
    47941 cagaccatgg ccgcccgcgt ttcttcgacg ccgatgggtt ctcccgtgcc gcaaccatga
    48001 aaacgaagaa tgtttacgcc gttcttcaag gcaatgtcaa agggcatgcg cttaccgcag
    48061 gacttgccgt aaccggttgt gacgccgtaa atgggaatgc ctgtgcgcat ggattgcatg
    48121 agcatgtccc gggttttttt catcttacgg acaaaggtcg catccttgct gattttaact
    48181 gcaacgccgt agcgcgctac ggcgatgatg tcgttgaaac ggattttgcc gccgtcaatg
    48241 atcacggcgc tcctgttgac gttttttttc atgaactatg aaaccccttt cttgtcccga
    48301 ataacctttg gacgaacgcg cggtttgtat gtgccgtccg ccttttcttt gtccatcact
    48361 tttttcaaca gtttgaacat cttgtaattg gccggctcct cctcatagaa tttatcccag
    48421 gcgtaataat aaagctcctg aagttttccc ggcgttaaga ctttgggctg aaaaacaact
    48481 ttcccgcagg tatagtcgct ccagtcgtag gagaaaatcc gcccggcatt gtgcagatca
    48541 tcgaaagtcc gtgtatgggg aaagggcgtc aggatggtaa attccgccat gtccagttca
    48601 atgtccagga gaaaatccac cagctttttg atgtagtctt ccgtatgata atccagtccc
    48661 agcagaatgc tgccttccac gccgatgccg aaatcgtgat aacgcttgat gcggtcgcgg
    48721 ataaaatcgg acgtatcgaa aaccgtctga taaacaaacc aggctccggc ctgcgcggcc
    48781 agatccagaa cggcgtcgtc tttttcaatc gggtgacagc accacttctt tttcagcggg
    48841 atcatttcct tgaataactg tgtttcccag tttttatcct gcgccagcga attgtctaca
    48901 atgaacagcc ggttgttttc aatgctggcc atttcttcga cgactttatc gaacgggcgg
    48961 ggacggaatt gcctgccgcc cagaaatcgc actgcacagg gatagcagtt aaaccggcag
    49021 ccccgggacg catgaaccag atccaccatc tgcacaccgc ggtaattgta catgtcgcga
    49081 tccagaatgc tgcggcgggc ggttccgacg ctttcaatcg gcggaaagtt ctgcatgtag
    49141 ttgtatacct tctgcaggtt gccgttttta aaatcatcga agacctgctc catccggcct
    49201 tcggcctctc cgagaaaaac gctgtcggcg tgttgctggg tttcttcggc gtgcagcatg
    49261 gtggcgatgc cgccgaaaat caccttcatt cctttttcac ggaatcggtc ggcaatgtcc
    49321 caggcgcgtt tgatctgggc agtcagcatg acggaaatgc cgacaaaatc cgcaggctca
    49381 tcgaaatcaa tatctttgat gttttcgtcg atgaactcaa cgtccacatc gtcgggcagg
    49441 gtggcggcaa acaccacggg tccgtgcggc ggaagggtaa atcgcgtctg tctgtccagc
    49501 ttctcccatt tcggataaat caatttaaac ttcatcagtt cactttctgc tcggttattt
    49561 tcaatgttct caccagatgg tgtttacaat attttatagc atacgccaat agaaaattgg
    49621 accgtgaaaa aatacggtct tggtcataat ttgctgaaga ttaaatagca atatgtgccg
    49681 ccgaaggaaa ttccggcaag cgcggtatga tgaatgtccg cttctttttt ctcctgtatg
    49741 acaaattcaa gcgcacgcac gggtttggca agacccgccg tcggcggcaa gctttgattt
    49801 gccatcgaca gagccgccgc gcaggctttg atgccgccgc tggaaaagct ctctcccgtt
    49861 gctcctttaa tcgacgtgat gcaaggacgt tttgtttcgt cgaatatttc ggcatacgct
    49921 tcagcttccg ccgcatcgag aattttatcg ccgttagccg ccgcaaaaat tgcctgcaga
    49981 tcgcttatcg tgataccggc gtttttcacg gcgcgggcga aggttctctt gatgccttcc
    50041 gtatcgcgcg gccattcatt gggcctggtg ggtgacgcgc ccatgccggc ccctgaaatt
    50101 tcgcaatagt agttgcatcc cctttgtttt gcggattgca gactttccag cagaatcatg
    50161 ccgcagcctt ctccggcaat acagccgttt ctttcctgat caaaaggacg gcagatttca
    50221 tttcctttat cttgaggcga aagaacgccg aatccgatca gcgagcgata ggcaatctcg
    50281 gataaaatat ccgatccgcc cgcaaagata aaatcggcgg tgccccgtct gatttcagcg
    50341 gcggcgtatg caattgcatt ttcggcagac acggcaaaat gcgtgatggt ggtattgatg
    50401 ccgcggaaac ccagctcaat agatgtatga ccggccgggg cgttcatcac cgtgttgggt
    50461 accaggatcg gattgacgga cgcaggaccc tcctgtaaca gcgtgtgcag aaactggacg
    50521 gttatatccg tggcgccgaa cgccgttccc agaataatac cgactctgtc gcggttttct
    50581 gccgtgatct gcaatccggc gtcttccagc gccatccgcg cggaggcaac cgtcatcagg
    50641 gagattttgt ccatccgccg catatttttc acggcaataa aatcccttgg cttaaactct
    50701 gtgacttcgg cccccagatg cgaagaaaaa gcaccggtgt cgaatgatcg gatgtctttg
    50761 atagcggtgt gaccggcaaa cagttttcga ccgaattctt ctttgccgat gcccaaaggt
    50821 gtcaccatgc cgataccgct tacaaccacg cgccgggcat catcttgcgg tcggtcagcg
    50881 ttcatgacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccgcttacaa
    51001 ccacgcgccg ggcatcatct tgcggtcggt cagcgttcat gacgtgcgct cctttccgtg
    51061 tatttgccga agatcagcgt ggtgttgttg cctccgaaag cgaaggaatt ggaaagcgcg
    51121 gtctttaaat ttgcggatct tgcgccgctg gtaacgtaat cgaggtcaca ctgcggatcg
    51181 cgttcctgat gattgattgt cggcggaacg aagccatcac gcagcgcgag gatggaaaca
    51241 atggcttcca gagcgcctgc agcgcccaga gtgtgcgcgt gcatggattt ggtggaactg
    51301 accggaatcg tataagcccg cttgccgaaa acatttttga tggctttggt ttccatggca
    51361 tcgttgaccg gtgtggctgt gccgtgcgcg ttgatgtaat cgatatcatc gggagacagt
    51421 ccgctgtccc gaagcgccgc ctccatggac cgaacggcgc cggaggcctg ttcgtcgggg
    51481 gcagtcatat gataagaatc gcaggtgatg ccgtagccca gaatctctcc gtgaattcga
    51541 gctcctcttg ccagcgccga atccagctct tccaggatca cgatcgccgc ggcctcaccc
    51601 agagacaggc cgtcccggtt tttatcaaat gggcggcagg tagcagcggc aaccgatttg
    51661 agtgcgttga aggctgcata tgtcagccga cacatcggct ccacgccgcc ggcgatgaca
    51721 acggacgcct ggccgccggc aattaaatca cgcgcgtagc caatggcggt ggcgcctccg
    51781 gaacaggctg tcataaacgt tgctttcggt cccgccaaac cgaaagccga agcaatacgg
    51841 tcggcggaag aagcgcagaa taaagaagca agccgggaga accgggcacg ccgtccgtta
    51901 ctcttcaaaa aattcgcgta aaaggattct gcttcgagca gtccgcccgc gccgccgccg
    51961 atcgatacgc ccgtgtcctt ctgaagttcc tccgggaaag gatataatcc ggcatcatgc
    52021 aaagcttcca gtgttgcggc gaatgaaagc agatctgcgc gcgacattct cttcatggag
    52081 aaattctggg gaatgtagtt gcgggcggca aaatttttaa tctggccgcc tgtccgggca
    52141 gaaaatcccg atgtatcgaa aatatcgatt tccctgatgc cgcaatgacc gtttttcagc
    52201 gctggcgaga aatcggggat attatgggcg atggcgttga gcgttcccat gcccgtgata
    52261 acaattcgtt tttgcaaatt caaccctcat ctgattttta cggcgattct aaaaatagat
    52321 gtaaaactta aattgacgtc ggtaaaaagt caaatgaata ttccccagtg acgatgattt
    52381 tccctctttt tgccccgctt ccgtcttgcg tttccccaat ccggatacgg ttgtccggca
    52441 gggccttcgg gaaacggtcc aaaaaaccgc ttgttttttt ggtatcctgt tgcgataata
    52501 ttaggaagac tatcatttga tattttaatt attaggagat cgtgatggcc aaaaaaaatc
    52561 ctgaaacaaa atatgaagaa aaagtcttcg tcaaagaaag gatcatcgcc aaacgactga
    52621 aaaacagtaa aaaggaggcg gctgatcaag aaattaaagc cgcaagaaaa gcggaacgtc
    52681 tcgatgaaga aaacgaggtg ctgattacca ttctttccga aatagaaaat cttcccaaag
    52741 aagatatcct ctataattac agcgaagaca tttccatgac cggaacccgg attcagggaa
    52801 attgtctttt gcccgtcgat acatttttga aaatcgattt agtattgaaa aatttgaaac
    52861 aaacggtaac ggtattcggc aaggtgaaat ggagcaagcc cgcagaagac gtcaagtctt
    52921 acgaggcggg cgtggagttt gtggatacgc ccgaggagtc catcaaaaaa ctgggagatt
    52981 atatcttgtc gatcaatcag tataaaaatc ttaatccggt cggggtgccc tactggattt
    53041 ttgcaaaatt caacaaacct tcatcaaaat aaatttttat cggtccgttt caattttcaa
    53101 atccgcttta catcgctttt gccaaaaagc atttcgttct tatctttttg tatgtttcat
    53161 taaacgtctt gaacaattta ttaaaaaaat ttcggataac ggtctcagag aaaaaatccg
    53221 gccgatgtca aaagacaaat attttattat ttcaatgaca tagaaaataa cgatcctttc
    53281 acgcaaactg gcacgataat ttcaataaat ataggcaaca acgcaggaaa ggaggtaaca
    53341 gatcatgaaa aagacattaa gaacaatagc ggtggcagcg gcggttgtta tttttactgc
    53401 aacgttcggc tttgcggagt acgcggcaac aggcgcggct gattttccct atttccaact
    53461 ggggtgcctg attgtcggcg ggttgatctt gatgactctg aaaaggaaat atgaaaaaat
    53521 gtatgttgcc gaaatggttg gtgcgtttgc tctttacacg cttttgatgg cattgttcac
    53581 caatccggtt atcgatgcag ttaaaactat ggttatgtag cacgtgttaa aaagatttga
    53641 aggaacgaaa acagaatgta aataaccgct atcaacaaaa atcatgatct gtttttatag
    53701 tggttgcccc cttgaatcgg aagattcaag ggggttttta atgatgttga ttgaggattg
    53761 tcctttatcg gaaaaaaagt tatagggata aaaagttcgt ctgaaaaacg aacaattatt
    53821 gacagatctc atggatgaat cgggttgatc atgaattttc tggccctgga ttttgaaacc
    53881 gccaattatt atcaggacag cgcctgtgct ctcggcctgg tgcgtgtcga agataacaaa
    53941 atcatccacc ggacatcctt tctgatcagg ccgccatacc ggtattttgt cttcacacac
    54001 attcacggta ttacctgggg cgatgttgaa gatcagccga ctttcgatca gtgctggaaa
    54061 tcgattaagc cgttttttga aggcattgat tttgccgtgg ctcacaatgc cggcttcgat
    54121 aaaggcgttc tgcgcgcctg ttgcagaaga tatgctatca cgcccccgga ccttgatttt
    54181 caatgtacga tgaagctttc ccggaaggta ttcaacctct atcccaccaa cctgcccgcc
    54241 gtttgcagaa atttcaatat tgacttacag catcacgacg ccttgtccga tacgctggcc
    54301 tgtgcaaaaa ttatgataga aattataaga aaaacaaata cttaaaaaat actgagaaaa
    54361 tcctcaatct aagttgagta attcctcaaa tttttgttga catgtccctt caaggttgct
    54421 atgaggtaaa aaaatcaaaa ttttattttt gtgagggact aagagagtct tcttaagcta
    54481 ttgttatttt aatgataaat taaaaaatag ctctcatttt aataaattta cataaaaagc
    54541 cctcttttaa aattctgatt tgcgcagccg gaaccgtcac acgtggtctc ggtctttggt
    54601 atctatttga aaaaacaaac atgaaatggt gaagcgtcca tgaccagcaa gcagaatatc
    54661 agggtggtga tcaccggagt cgggttgacg gctcccaacg ccaatcatct gaacgatttt
    54721 cgccgcgccc ttttaaacca gaaatccgga atttcggaaa tggaagtccg atatatggga
    54781 aaggttgccg cgggtaactg taattttgac gaatatcttt atcagaagaa aaaaatgcgt
    54841 cgccggggaa cccgggccgg ttctatttcg atttactgcg cgcacgaggc attgaaagat
    54901 agcggtctgt ccctggaatc actggtcaag gacagaaccg gcatttacct gggcattacc
    54961 gagcacggca atgtggaaac cgaaaatgaa atttatgaac tccaccagaa taatctcgat
    55021 gccggtttat ggtctcatca tcacaatccg agaaccgtgg ccaacacgcc cgccggcgaa
    55081 gtcagtctga atacgggaat taccggtccc gcctacacga tcggcgcagc ctgcgcagcg
    55141 ggcaatatcg gcgttattca cggcatgcag atgctgcagc tcggtgaagt cgatctggcc
    55201 ttggccggag gcgtttcgga aagcacggga acattcggca tctttgcggg attcaaaagc
    55261 cagggtgcct tgggttctca tgaagatccc acaaaagcga gccgcccgct ggacatcaac
    55321 cgaaacggca ttgtggtttc ggaaggcgga gccgtttttg tcctggaaag actggaggat
    55381 gccttgcaac gcggagcgaa gatttacggg gaaatagcgg gatatcatgt caattccgat
    55441 gccgtcgatt ttgtgctgcc ggatacccaa cggcaaattc agtgcataaa cacggcaatg
    55501 gccaaggcaa acatctcccc ccgcgatatt gatatcgtta atctgcacgc caccggaaca
    55561 gtgtccggag atgaaaacga gtgtgaagcg gtcagggcgg ttttcggaca atccgataac
    55621 acctacgtca attgcacaaa aggctttatc gggcatgcca tgggcgccgc cggcgctctg
    55681 gaactggcag gaaatttgcc gagcttcacc gatcacaaaa ttcattcgtg taaatatatt
    55741 gagaatgtcg atccgaagtg taacgtgaaa aatctggtta acggcgatcc cgtcaaaaaa
    55801 gacgtgaact ttattttgag caattcgttc gggatgctgg gcataaattc cgttttaatc
    55861 gtaaaaaaat acaaacaata aaattataag gaggcagggg catggactat caaaccataa
    55921 gaaaaaaaat catcgatatc atagctgaaa ttgcgattga tgaagacctt tcaaatcttg
    55981 acgacaacat cagattaagg gaacaactgg atatggattc catggacttt ctggatgtgg
    56041 tcatggaact gaagaagaga tatagaattg aagtgcctac cgaagattac atgaagctgg
    56101 caacattgaa gagctgtatt gaatatctcg gccccaagtt tgctgtaagt ccggcataat
    56161 gacacaatat gatacggtaa tcataggcgc gggcatgtcc ggtctggcgg ccggcatccg
    56221 gctcgcgatg tttgatcaaa aagtctgcat cctggaacgg cataaaattc ccggcggact
    56281 gaattcctac tatgaccgcc agggccggaa actggatgtc gggcttcatg caatgaccaa
    56341 ttttgcccgc cagggtgaac ggggccggcc gctgaccaaa ctgctgaaac aattacgaat
    56401 accttacgaa cagctggaac tctcgcaaca gaatttttcg cgcattgtgt ttcctgcaca
    56461 cgatctcgca ttcgctaatg atccgcagtt gctgatacag gacatcggaa aacactttcc
    56521 ccatcagaaa gatccttttg ccagactgct tgacgtagtc atcaattttg atgacgttaa
    56581 tctgagcaac aaaccgcaaa gcgccaaaga aaaagttcgg gaaattatct ccgatgagta
    56641 tttgctggaa atgctttttt gtcctctgtt gatttacggc agtgcgtggg aaaacgatat
    56701 ggatttttcc cagttcgtca ttatgttcaa aagtatttat ctggaaggct tcagccgccc
    56761 ccgaaacggc gtgcgcacca tcatcagtct gctgattgag aaatacaaac aagccggcgg
    56821 cgaattgcgt ctgggaacgg gcgtcaaaaa aatagatgtg gaaaatgaaa gcgtaaccgg
    56881 cgtaacgctt gattccggag aaagcatccg gaccgacaaa ataatgtcct ctatcggctt
    56941 gcccgaaacc atgaacattg tttccgaaaa aagcgacagg cctgcagtcg gcaacatgag
    57001 ttttgcggaa aaccttttat tctttgacaa aaagcccgtt gaggcgggca tcaaagaaac
    57061 cattattttt tacaatgaca atgatacgta cgactacaaa aagtcccggg atctttgtga
    57121 ttatcgcagt gccgtcattt gcctgccgaa caatttcgga gccgatgatt acgatgaagg
    57181 attaattcga atcaccttta ttgccaattt tgatcaatgg aacgcgttgg acagaaaaac
    57241 atatcttcaa aagaaaaagg aagtatgtaa tgcggctttg tcgcttgttg caaaagtgat
    57301 tccgcaattt caggccaagc tgctttatca tgatgttttc acacccaaga ccattaaccg
    57361 cttcaccggt catttcaagg gcgcggttta cggcacaacc gagaaagtca gagacgggcg
    57421 caccggcata aaaaacctgt ttatctgcgg cacggatcag ggctttctgg ggatcgtcgg
    57481 cgccatgctc agcggtattt ccatggcgaa tcttcattgt ctgatgaacg gagaaaaagc
    57541 atgaggttta aaaacgcggt catctcttct tttgcttatc atcttcccga ccaggtcatg
    57601 acgtcggaag aaattgagga gcggcttgcg cctctttatg aacggctcaa actgcccgcg
    57661 ggacgggtcg aactgatgac cggcattaag gaaaggcggt tctggccgcc cggaaccatg
    57721 cccagcgatt tatccacgca ggccgcccgt aatctgtttg agaaaaacat cgtgcagccg
    57781 gaccagattg atttattgat tcatgcctcc gtctgccgcg attttctgga gccatcgacg
    57841 tcttcgatcg ttcatcacaa tctggaactg caaacggata tcatgttctt cgatctgtcc
    57901 aacgcgtgtc tgggcgtcat gagctcgttt gtcgttgccg ctaatatgat cgacaacggg
    57961 ttcattaaac atgccctgat cgtgtccggc gaaaacggcg gaccgctgct ttttcagacc
    58021 attgaccggt tattaaatga taaaaatata accagaaaaa gcatcaaaaa atatattgcc
    58081 aatctgacca tcggctcagc cgctgttgcc tatacggttt ctcacaagga tcatgtccat
    58141 gaaggtcatt gcctgctggg aggagcctcg cttgtcgatt cttcagccaa cgtcctatgt
    58201 cgcggcggcg gagacacgca atccttgatg atggaaaccg attccgagga actgatgaaa
    58261 tccggcgttg ccctggctga aaaaacctgg gcagaaacca aacgggaact ggggtggacc
    58321 cacgatgacg tgaactgctt tgtcggtcat caggtgggtg tggctcatga aactctgtta
    58381 aagcagattc tgaaactgga gcattgtccc acgtttacca cgtatcaatt tttgggcaat
    58441 accggagctt ctgcgctgcc cgtaacactg gctatttatg atgaagagaa aaaaattccc
    58501 gcaggcagcc aggtgggttt gttgggcatc gggtcgggcc tgaattccat tatgctgggg
    58561 gtaaaatggt aagaccggac tggttgaaca aattatatcc tttcacatcc catttctatc
    58621 aactcaagga taatatttcc ctgcattacc tcgatgaagg acaaggtgat ccgatgctga
    58681 tgcttcacgg caatcccacc tggtcgtttt actacagaaa actggtggcg gcgttttcca
    58741 tatccaacag ggtgattgtc ccggatcaca tcggttgcgg tctgtcggat aaaccgcagc
    58801 aatatgccta taccttgcat caacacatca ggaatctgga caaacttgtt tcctatctgg
    58861 agttgaaaaa cgttgtgctg gttgttcacg actggggagg ccccatcggt ttcggacttc
    58921 taaagcgacg tccggaactg gttaaaaaaa tagtgatcct gaacagcgcc gcttacacgt
    58981 ccaaggtgat cgcctttcag attaatatgt gccggattcc cattttggcc gaacaggtga
    59041 ttcgccgtct taacggattt gccctggctg caacgtacat ggctgttgct aaaaaaatgt
    59101 ctcctgaaat cagaaaggga tatttgtatc cgtacgacaa ttacgaaaac cggattgcca
    59161 tagcgcagtt tgttgccgat attcccatga cgcccggaca ccggagctat cagacgttaa
    59221 aatctgttga attatcgctt gcgcaacatc gatgtcccgt gcttattctc tggggaaaaa
    59281 gggatttttg ctttcacgat tattttctca atcgctggca ggaaatttat cctcacgcga
    59341 ccgtaaaaac atttgaggac gccggccatt atgttctgga agacgccgga gacgacatca
    59401 ttgcagaact cggaaagttt gtttgaataa aattgcatga cggtgaaaag ctgtaaccat
    59461 gaacatcgct gatcgtttcg aagagatagc aaaaattcat cacgacaaag ttgcggtcaa
    59521 gtttcccgtt cgcacgggca aaaagtaccg ctacaaagcc atcaccttcg gggaactgga
    59581 gacgcttgcc ggcaaatacg ccaacggtct ttctgaaatc ggatttcgtc gcggcatcaa
    59641 aacgcttctt tttgtccggc cgtcattaaa atttcccgcg ctggtttttg ccctctttaa
    59701 aatcggcgtc atcccggtgt tcattgatcc cggcatgggc agaaaaaatc ttcttgcggc
    59761 cattgaagaa gtggctccgg aaggattgat tgcagaaccg gaagtgcatc tcctgaggat
    59821 tcttttcaat aactcgttta aatcggtcag gttcaaagtt accaccaaag gaccgaagat
    59881 aggcaattgc ttcccgctgt ccatgttgaa acgggccaga gccggttttc aaagcgcgca
    59941 gctggaaccg gacgaaacgg cagccattct gtttacctcg ggcggaaccg gctgcccgaa
    60001 aggcgttgtc tatacccaca aaatttatta cgagcagact cgtcttttac aggagctttt
    60061 ttctctcgat gaaaatgaaa tagatttccc ctgcttcccg ctgttttctc tctttacgct
    60121 ctgtatgggc atgacgtcct gcattccgga tatgaatccc tcgcaaccgg caaaggcgga
    60181 cccgaagcaa ttggtgcaaa atatcaagga caataaagtt acctttatgg ccggatcgcc
    60241 ggctatctgg gaagcgctgg gaagttactg cattaaaaac aacattaccc ttccttcggt
    60301 gaaatatctg gtgatgttcg gtgcaccggt cagaaacgaa ctgcataaaa gattcagcag
    60361 gattctgccc aacggaacca cctatacgcc ttatggcgca acggagagtc ttcccgtagc
    60421 caatatttcc ggacaaatgg ttttggagca aacggccgcg ctcaccgatg aaggcaaggg
    60481 aacctgtgtg ggagagcctg cccccggcgt gaaagttgcc attgcggcga taaacgacaa
    60541 cataattcct catatccggc aggcgcgcat attgggcctg catcagatcg gagaaatcat
    60601 tgtgaaaggc gacatggtta ccgccgggta ttatcaatct ccgcaggaga ctgccctggc
    60661 aaagattgcc gacggcgcat cgttttggca cagaatgggc gatctcggtt atattgatga
    60721 tgcaggcaag ctctggttct gcgggagaaa atcgcaccgc gtcgaaacac cgtcgggatt
    60781 attatgctca gtcatgtgcg aagcgatttt caacacgcat aacggcgtca gacggtcggc
    60841 gctggtcgga atcggagaaa aaggcaggca atcggctgcg atcgtcattg aaagaaattc
    60901 ggcagggcga aagcttgaca ggaaacgatt ggaaaaagag ttattggatc tgggcggaaa
    60961 gaatcctttg acagcggcaa taacgtcagt ccatttctgc gacagctttc ccgtcgatgt
    61021 ccggcacaat atcaagatcg accgtttgaa actggctgat gacgttgccc gggggaggat
    61081 atgaacatta tcgttaccgg agcgggaggt tttctgggaa gaaatctggc aaacggcttg
    61141 ctgtccaaag gacacaaggt ccggaacttc agccgcagga atcatctgcc attgcaggcc
    61201 atgggcgttg aaactatttg cggcgatttg caaaacgctt ccgaggttat ggcggcattt
    61261 cagggaatag atgcggtctt tcatgttgca tcgcgcgtcg gtatctgggg cgactatgat
    61321 gaatattacc ggacgaatgt tgtcggcact ggaaatgtca tcaaggcgtg ccgcgccaac
    61381 ggcataacaa aactgattta tacaagcacg cccagcgttg ttttcgacgg caatgatatt
    61441 ttgagagccg acgagaccaa accctatgcc ctgaaatttt ataatgacta ttcccggacc
    61501 aaaacgctgg ccgaacagaa ggttttgcag gctaatgatg aaacattatc tactgtcgcc
    61561 ctgaggcccc accagatcat cggtccggac gatccgcatc tcgtccctcg actggtcaat
    61621 gcggcaaaac ggggcagact gaaaatagtc ggaaccggca agaatcttgt ggatgtcacc
    61681 tatattcaaa atgccgttga ggcgcacctc ctggcgttcg aaaagttgtc tcctcaatca
    61741 tctgttgccg gacaggctta tttcatcggc caggaacggc cggtcgtgct ctgggatttt
    61801 atcaacaaga ttctgacgcg atataatctt tctcccatca caaaaagagt tcccgaaaaa
    61861 gtggcgttga acatgggggc cttgttcgaa gcctggtaca gattttttaa aattaacgga
    61921 gagccgccga tgacgcgatt tgtggcgctg cagttttccc gctcccatta tttttcccat
    61981 gaaaaagcga aaaacgattt cggttatgtc ccgaaaatat ccatagagca ggcgctggat
    62041 cttacaacag agaggggaat gaatgctttt aaataaaaac gataaaatta cacaacacta
    62101 cgatgtcatc atcatcgggg gcggactggg gggattgacc gccgccaaca aactggccaa
    62161 aaacggaaga aaagtattaa tcctggagtc gcacgataaa cttggtggct ttgctacctg
    62221 gttttatcgc aagaaacata tttttgatgt gtcgttgcat ggatttccgg tggggatgat
    62281 caaaacctgc cgcaagtatt ggaacaaggc gatagcggag aaaatcgttc aacttaaaag
    62341 aatgcgtttc atcaatccca tgtaccgatt ggagacggat tatacggttg aagattttac
    62401 cgaaaagctc attgcctgtt ttaacgtttc taaagagcag gtggataatt ttttcagcct
    62461 ggtcatgaac atgaattact acgataacaa ggcaatgacc aacggcgaac tgctggagag
    62521 tttcttccca ggcagaaaag acgtgcatcg ttttttgatc gagccgattg tgtacgccaa
    62581 tggttcgacg ctggaggatc cggccattac ctacggaatt gttttctcca atttcatgag
    62641 caaaggcgtt tatacgtttc agggcggaac cgacctcgtc atcggaatga tgcaggagga
    62701 gcttatgaaa aataatgtcg atattcgtct gaaatcaaac gtgggaaaaa ttgtggttga
    62761 aaacaatgtt tgtcggggag tcatgctcgg cggcgagttt gtttcttcca cgtcggttct
    62821 ttccaacggc aatctcatcg ggaccatttt taatctgacg ggagaagata attttaaacc
    62881 ggaatttatc gacaaggtta aaaaagtccg gctcaacacc agttcctgtc aggtgtatat
    62941 gggaatcaga cagggagaag cgattgagga tatcggcgat ctgatcttct actcggaagc
    63001 aaaggaattt gaaaccgatg agttgctgtc catgaacacc accagtcgga cattctcctt
    63061 ttactatccg cgactgcggc cccaactgga aaatgccgga tataccatcg tggcatcgac
    63121 caatgcgcgc tatgaagact gggcagaatt aagcaaagaa gattataagc aaaagaagcg
    63181 ggaattgatt gaggcaacgc tggtgagtct ggaaaaaatt attcccggca tccgacagaa
    63241 aattgactac gtcgaggcgg caacgccgtt gacgatcaag cgatatacgc atcatgaagg
    63301 aggctcctcg ttcggaacca aacacgaggg cttggatatt tccatgaaac tgcatcatga
    63361 aatcagcggt ctgtttcata ccggatcggt cggcattatc atgtccggct ggctgggcgc
    63421 cgccaattac ggcgtcattc aatcttatga agtggaaggc tatctggata aacttaattg
    63481 aggtgtttta tgtttgattt cagcaatcaa agagttattg ttaccggcgg aacccgtggg
    63541 atcggagcgg gtatcgccga ggcttttctg caggctggcg cgacggtgat tgccgtttat
    63601 tcagccaacg atgcggccgc gaatgaattt aaaaataaaa atgaacaatt ttcggataga
    63661 ctcgatattc aaaaacagga tatctcctcg tcgcaggatg ttaataattt gtttcagtat
    63721 ctggatgaaa aatatccctc attggaagtg ctggtgaata actcgggtat ccggcaggac
    63781 gcgatagcgg cgttaatgac cgatgagcaa tggaataaag ttttggaagt taatctgaca
    63841 ggaacgtttt atatgtcgcg ggaaattatt ccgcgcttta tgtccggtcg tttcggacga
    63901 atcatcaaca tcagttcgct gggcggggat atcggatttc aaggacaggc caattacgcg
    63961 gcatccaaag ccggccaact ggccattgcc aaatctttgt ccaaagaggt cgccaggaaa
    64021 gggattaccg tcaactgcgt ttctccggga tttatcgata cggaactcat cggggatctc
    64081 ccagaggagc agcgcaagga atacctcaag atgattccgc agaaacgttt cggcaaggtg
    64141 gaagaagtgg cccacgcggt gttgttcctg gcgacccgcg aggcctccta cattaatggg
    64201 acatcgatca ccgttgccgg aggactataa tggacgaagt tctggctgct ctgccgcagc
    64261 gtccgccgtt tctttttgtc gataaaatta tctgcagaga cggtcgttcc atcacgacgc
    64321 aacgaacgtt aagagccgat gaatattttt ttcagggcca ttttcccggc aatcccatca
    64381 tgccgggcgt gttgttgtgc gaagcctgtt tccagaccgg agccatttta atggtagcaa
    64441 gtcatcagcc gggcctgggt gttgtcaccc ggattagaga tatcaaattt aaacatttcg
    64501 cccgtccggg cgatacgttg accatgaagg ttacccttga tgatcaggtg gataacgctt
    64561 attatatgag cggtacgatt catatcggag ataaactcat tttaaaaatc atctttcagg
    64621 cggcgcttct tccgccggag caataagaga ggttgatatg gactttctgc aagtatcagg
    64681 aaaaacattt ctggtgaccg gtgtggccaa taaaaagagc gttgcgtttt ttgtggccaa
    64741 aggtctggtc gaaaacgggg cgcaggtgat tttttccgtt caaaatgaag aaaataaaga
    64801 cagagcgcaa aaacttttcc ccgacagcac cgtgttgatc tgcgatgttg aaaaaacggg
    64861 aagcatcaat cgtctgggag aatattttaa aaatcataat atcaaactcg ccggtttcgt
    64921 tcattccatc gcctttgcca attacagcga aggcctcaaa ccatttcatg aaaccaaacc
    64981 ggaagatttt ctgcaggcgg ccaatatctc ctgcttctcg ctggtggccg tcgccaatgc
    65041 cctgaagaat tcttttgaaa acgatgcttc cattgtcacc atatccatca gcaacacgcg
    65101 ggcaaccgcc tatgggta
//
LOCUS       DAONQZ010000007        62347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_498_length_62347_cov_15.477124, whole genome shotgun sequence.
ACCESSION   DAONQZ010000007 DAONQZ010000000
VERSION     DAONQZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 62347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62347
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_62347_cov_15.477124"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..2668
                     /locus_tag="PLR13_02545"
     CDS             518..2668
                     /locus_tag="PLR13_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC18240.1"
                     /translation="MPESDMDNTTPYDIQYLFEPKSIAVIGASQNKKKIGYAVFNNIV
                     SGGYQGNVFPVSPKGGDVDGHKIFSDILDIDEEIDCASIVIPADLVKDAVKKCALKKV
                     KFLQIITSGFSEVGNVHEEKEIFDIASQAGTRIVGPNMFGLYSRAASLNSTFSAAKIS
                     PGHVAILTQSGALGIAMISKTAVANLGLSAIISIGNKCDVDEADLLAYLIQNDDTKVI
                     FIYIEGVKKGERLIEALKVATGKKPVIVIKSGKSKRGAQAAASHTGSLAGSDDIFDAI
                     MKQCGVLRAECLEDAFNWCKFMISGSAPRTRNTVIVTNGGGVGVMATDACEKYGVSLY
                     DDQAALRGLYEAVTPSFGSTKNPIDLTGAAGGTEYTRAFSVTAESEDIGSTIGLYCET
                     ATFDSGNLAQMIQETYARHQKNGKPISYALIGGKQVENAITALIAERIPVFNDVEQAV
                     SCMGAFYRYHNYLDEVSRKIDEYAVDTAAINAVIDRAAGAGRTFLLANEGAAVLRAAD
                     IAMPQSKIARSIQEAVQFAGEIGYPVVLKVVSKDILHKSDAGGVLLDLDDEKEVIVGY
                     EAIMHHCRMYKPNAVIEGIEVAEMVKKGIELIIGARRDPSFGPVIMCGLGGIYVEIMK
                     DISFRAVPINRSIALSMLEEIRSYPLLLGARGEEKKDIESVIDTIIKIGSIIRTCERI
                     TDIEVNPIEVYEAKSGIKAVDARILISRMKEDTQ"
     gene            2665..3732
                     /locus_tag="PLR13_02550"
     CDS             2665..3732
                     /locus_tag="PLR13_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQC18241.1"
                     /translation="MKKIVVSSMRRNSGKTSIIAGIVSLMHDKKFAYAKPLGDRLIYR
                     KKRSWDYDASLMVNLLGRQGEMESHCEKITLGFDHSRLKYMYDKEGIERALAEIVTDI
                     GAENDVLFIESGKDLTAGAYLNLDAVSMTKSIDGVLVIVVSGDDDCVLDDMRFIEKYV
                     KLEGVHFGGVIINKISDIEEFEASCTPVIREMGLDVIGAIPYEEPLTYFPLDFLADKL
                     LARVLAGEEHLKNIARTILIGTSATFVADKEQIPARPELSGTGQLMITGGDRSDLILA
                     ALERDTAGIILTNDIVPHQNIISRANERGIPVLLVGTDTFQTARTIDDMEPLLRKQDT
                     GKISLLSQLIEKHTNMAEFLK"
     gene            4050..5945
                     /locus_tag="PLR13_02555"
     CDS             4050..5945
                     /locus_tag="PLR13_02555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC18242.1"
                     /translation="MEPEIFEEYLAGFKKAYPKKFVSEDEIFSHIRRGDHIFVASGCG
                     EPQYLVKAMIRYVESHPKAFFDAEIMHVYSLGLAPYTEPKYKANFRHNSFFIGNSTRS
                     SINAGMADYTPISLSQVPSLLASGVVQIDVALIQTSMPDEHHYLSLGVSVDVVKAATE
                     KAKIVIAQINPNMPRIHGDGFIHIRDVDFIIPYDEPLLEVSSPKTGDVIITIGKNVAR
                     LVQDGDTIQVGWGSLPNAVMFCLSNKKNLGVHTELLSDGLVDLIKAGVINNSRKTIDH
                     GKTVASFCMGNKSTYAFLDNNPSISLRTLDYTNNPVMMSRIDNMVAINSALEIDLSGQ
                     ATSESIGGIFYSGIGGHQDFMRGALFARNGKSILALKSTSKNDTVSRIVPALKEQAGV
                     TLNRGDVRYVVTEYGIAYLHGKNIRERAMSLIAIAHPKFRPWLIDEAKKRGLIFKDQA
                     FIPGKRGEYPEDLETYLTTKTGLQVFIRPVKISDESLLKDFFYSLSDKSIERRFISTR
                     KDMPHERLQNFVIIDYTKQIVLVAIKGDVDNSYISAVGQYGIDDATHTAEVAFAVRDS
                     EQNNGIGFELLKYLTYLAKRQGLLGFTAEVLVENKPMLHVFEKGGFDIKKKIDSGVYE
                     LTLKFKL"
     gene            5970..7307
                     /locus_tag="PLR13_02560"
     CDS             5970..7307
                     /locus_tag="PLR13_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQC18243.1"
                     /translation="MNPHVFREYDVRGVVDKDLNEDFVFHLGRAIGTYARAKRIRNMT
                     VGRDCRLSSESYHRFLSRGLNAAGIHVIDIGMCATPMLYFSIRHLRVGGGVMITGSHN
                     PPEFNGFKICIGHSTIFGPQIQDLRKIMESGRYAKGAGRCREKDIAKEYENYLFKRIK
                     IQRRIKVVVDGGNGVGGYFAVPLLKRFGCEVIPVHCEPDGTFPNHFPDPTVEENLTDL
                     IRLVRKNKADLGISFDGDADRLGVVNDKGEIIWGDKLLLLFARHILKNHPRAAIIGEV
                     KCSQVLFDDIEKHSGKAIMWKAGHSLIKAKMKEVKAVLGGEMSGHFFFADRYFGYDDG
                     IYAALRLLEILSRTGKKLSGLFADVPKTFATPEIRVDCTDSKKAKVVEGIKQHFKNTP
                     GIVDIDGIRIPFENGWALVRSSNTQPVIVLRFEAASVKTLNKIRGEVEALLHV"
     gene            complement(7366..8331)
                     /locus_tag="PLR13_02565"
     CDS             complement(7366..8331)
                     /locus_tag="PLR13_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18244.1"
                     /translation="MFALSPAITPAARRERSFHAAPITMSGTLYPFKVNDSLWILGNG
                     YFHLYLLKGAKQRALVETGISATADLVLSQLEALDVRPDYLVVTHPHSDHITGLDHLR
                     GAFPGAVVMIAAGAASFVAHPKAAPAIIAEDRHMFRALAARGLFSQTAVVTMPPSLSG
                     CRILTDGEALDLGGMSVNFLSVKGHSPGNLLAWIAGTKTLLVSDSLGNHYPGRGFFPT
                     FFTGYNDYMATIDRLESFRPKRMGLAHNGFFSTSGAIADIFCLSRKSAQDVRRYIEDC
                     AMDDEDLARSLFGFYYTDELAVYSPDNILNCCRLLVRRVRELHTA"
     gene            complement(8386..9348)
                     /locus_tag="PLR13_02570"
     CDS             complement(8386..9348)
                     /locus_tag="PLR13_02570"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HQC18245.1"
                     /translation="MERFRKLCVIILSLSFIFSVAACDNFSKFKEKMLSEEIISFPVK
                     QGNSWGFIDTTGKFVIQPQFDGAGRFSDGFAAVKIGDRWGFIDKTGEYAINPQFDVAF
                     LFHDGLASVGLGGGKVKVGFINKQGKYVINPQFDDAGWFSEGLTAVGKIDGQKVKAKV
                     GFIDKSGKYIIKPRFEDAGWFSEGLAAVKIDGKYGYIDKTGKFIIEPQYDLAFWFSEG
                     MATVKNGNKYGYIDKTGKFIIEPQYDFAFWFSEGLASVKSKGKYGFIDKSGRYVIEPQ
                     FDIAFWYENGLAPVGIGRGKVKVGFINKQGKYAINPQFDGVNVF"
     gene            complement(9598..9674)
                     /locus_tag="PLR13_02575"
     tRNA            complement(9598..9674)
                     /locus_tag="PLR13_02575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9638..9640),aa:Pro,seq:tgg)
     gene            complement(9678..10274)
                     /locus_tag="PLR13_02580"
     CDS             complement(9678..10274)
                     /locus_tag="PLR13_02580"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HQC18246.1"
                     /translation="MKIVFASGNLGKVREIREMLSCINIELVSLKDYPDIPEIVEDGK
                     TFLENALKKARTVSERTAQAVLADDSGLQVDALGGEPGIYSARYAGEGASDDDNINLL
                     LEKLKEVPPEKRTASFCCVLVLYNTDGTYESFESRWPGRIIDDRRGDNGFGYDPVFYV
                     PELNKTAAELPPEIKNRISHRGQSFLKLKQSLGRKMKK"
     gene            complement(10271..11002)
                     /gene="rph"
                     /locus_tag="PLR13_02585"
     CDS             complement(10271..11002)
                     /gene="rph"
                     /locus_tag="PLR13_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQC18247.1"
                     /translation="MTERKSGRRFDELRPIKLIKNYLNTAAGSVMVEFGNTKVLCAAS
                     IDEKTAPFLRNTGRGWVTAEYSMLPMSTQTRTARESVRGKIQGRTQEIQRMIGRSLRA
                     VTDLSAFGEKTIYLDCDVIQADGGTRTASVTGAFVALVGLFDQLKRDGVVQNIPVRDY
                     LSAVSVGIIDQQVMLDLEYEEDSRADVDMNFVMTGSGHFIEVQGTAEKIPFSREQMTQ
                     MTDVAERGIRQIIERQKELVGDLIG"
     gene            complement(11006..11695)
                     /locus_tag="PLR13_02590"
     CDS             complement(11006..11695)
                     /locus_tag="PLR13_02590"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQC18248.1"
                     /translation="MFHTKKILVLITFAVLACFGWGAAELIAAEKKYIILIDPAHGGK
                     DRGLKLNDDVSEKDVTLAVALQIKKELAQEPNLQIILTRETDKTLDREARQKMIKTLN
                     PDMVLNLHVNGGFGKEASGYEIYYPSCGADDPASMRKTDKTKPQSQCQNNSLKMAKLI
                     SDNFNALFPRKARGLRMADLSVTEGVLVPAVAVEMGFATNAVDQKNLMSKKTQAEMGK
                     ALAVSIKAFSR"
     gene            complement(11760..13913)
                     /locus_tag="PLR13_02595"
     CDS             complement(11760..13913)
                     /locus_tag="PLR13_02595"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQC18249.1"
                     /translation="MLRINDIIEKAQTYLPAEDLEMIEKAYIYSASVHQGQVRLSGEP
                     YLTHPMEVAGILTDMKMDAATIISGLLHDTIEDTLTTADQLEHEFGKDVSFLVGGLTK
                     LSRISFGSQEERQSENFRKMILAMSSDIRILMVRLADRIHNMRTLQYHPKEKRIYIAR
                     ETMDIYAPLANRLGINSMRMELEDLAFQYIAPDAYNDLTAKIVKSNEARNRYTEEVKN
                     IIQQEMEKYSVACKVEGRAKHLYSIYKKMHEQHINFDEVYDLIAFRIILDADKEKSCY
                     EVLSIVHALWKPVPGRFKDYIAMPKANNYQSLHTTVIGPYGERVEIQIRTRNMHEWAE
                     KGIAAHWRYKEGGSYKDNEEDQIRKLREVLEIQQDIKNPKEFMSDLKLALFPEEVYVF
                     TPHGDVKSFPKGATPIDFAYSIHTDVGNQCIGAKVNRSIVPLRYKLQNGDRVEIITQA
                     GHHPSKDWLKNVVTTRAITKIRNYINLEEKKNSIVLGRELLEKEFKRHNLKFSGIVKS
                     DDIKKIFSAFSVNQLDDLLFQVGLGRISAKRVAHYFIAGEEVEAAEPAREKIKKKKAA
                     SPSMGVSLIGIDDPDDIMVNFAKCCNPIPGDDISGYISRGRGVIVHTSNCPYIKRMDQ
                     ERIVDVQWSSREEHTYPVNIKVVCDDIKGILTEVSSIISSFDVNISYAEIETVNMIAT
                     CKFIIDISNLPQLNQIMSAIRQLKFVKAVEWLHHF"
     gene            complement(13941..15668)
                     /locus_tag="PLR13_02600"
     CDS             complement(13941..15668)
                     /locus_tag="PLR13_02600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQC18250.1"
                     /translation="MRYSEMFLPTVREVPSDAEIISHQLMIRAGLIRKLTSGIYSYLP
                     LGYRVIRKLEQIIREEMNRAGAQEVHLPMVQPAEIWQESGRWTYYGKELLRLRDRHDH
                     EYCLGPTHEEVITGLVRNDVKTYRQLPLNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDVDEEGAEKSYEKMFAAYNNIFRRCGLQFRPVEADSGSIGGKYSHEFMVMADSGE
                     DAMVFCEKCAYAANLEKAEVACPEKKIIFEDDWLPLEDVDTPDVRTIEEVSAFLKVTP
                     QNIVKTLIFNADGKPCAVLIRGDHEVNEVKVKNYLAAAELELASDDMIREVTGAPRGF
                     AGPVNIKAPILVDYSVIDMVNFVTGGNRQDGHLKNVNVGRDFKVEAFTDLRIVNAGDP
                     CPRCGQPIKTARGIEVGHVFKLGTKYSKAMKAVYLGKDGQEKIMIMGCYGIGIGRTVA
                     AGIEQNHDDNGIIWPMPLAPYQVIITPVNINEITIRETAEYLYNAMITEGVEVLYDDR
                     DERAGVKFKDADLIGIPLRVTVGHKNLQDGNVELKIRKTGANELCPLQDIVGRVREII
                     RQELNPDIA"
     gene            complement(15668..16762)
                     /gene="ispG"
                     /locus_tag="PLR13_02605"
                     /gene_synonym="gcpE"
     CDS             complement(15668..16762)
                     /gene="ispG"
                     /locus_tag="PLR13_02605"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQC18251.1"
                     /translation="MATVEHSSFIRRKKTKQIHLGGVAIGGDAPVIVQSMTNTDTRNV
                     EATVRQIKALEKAGCEIVRIALPDDKAAASVSAIKKRIRIPLIADIHFHDRLALEAIR
                     QGVDGIRINPGNIAPGKMGAIIRLARERRVVIRIGINSGSLQKDLLKKYGGATPEALV
                     ESAVRNLELFEDRGFDLIKLSLKSSDVPATIEAYRAIAARTDYPLHLGVTEAGSLIGA
                     AIKSSLGIGTLLYEGIGDTLRVSVTGDPVSEMGLAYGILRALGIRRVGVDIVSCPTCG
                     RCEIDLPSLTRRIEKALAGRKDYLKVALMGCVVNGPGEAADADIGLAGGKGQGLLFKK
                     GKAYKKVREKDFVNILLEEIDTIIEERKKD"
     gene            complement(16857..18260)
                     /locus_tag="PLR13_02610"
     CDS             complement(16857..18260)
                     /locus_tag="PLR13_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQC18252.1"
                     /translation="MGGIFGVVSSANCIKTLFYGTDYHSHLGTEHAGMAVLGPQGFYK
                     TVHSVSQVQFKSRFIDEYKKMDGFFGIGAIDDDTPQPLIFRSRFGVYAVAAVGLINNK
                     NQLVNELLKEGANFGERAGGDINNAEVVSCLINRGDNIVEGIASMRAVIEGSICAVVM
                     TSEGIYAARDKFGRFPLAIGKSKNPQDQSYVVATEASSFENLGYELTQFLGPGEIVLL
                     TTDGVKPVKPAGTEKKICSFLWIYTGYPSSSYEGISVEGARERCGAYLAKRDNIQADF
                     VSGVPDSGVGHALGYAMASGIPYRRALVKYTPGYGRSYTPPAQEIRDLVATMKLCAVR
                     EVINGNRIIICDDSIVRGTQLKNLTIKKLWDNGAKEIHVRPACPPLMFPCIYTSSTRT
                     TGELACRKAIRALEGKEIEHPSEYLDHHSEKHAKMIDWIRRDLNVTSLKYLAIDDMIA
                     AIGLPADQLCLHCWLGK"
     gene            complement(18277..19470)
                     /locus_tag="PLR13_02615"
     CDS             complement(18277..19470)
                     /locus_tag="PLR13_02615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQC18253.1"
                     /translation="MRLSARVGRISPSETLAITAKVNALRAQGRDVIGFGAGEPDFDT
                     PDNIKDAAIKAIQSGFTKYTPVGGTDELKDAIINKLKRDNRLEYKRSQIVVSCGAKHT
                     LYNLAQIYFDAGDEVIIPAPYWVSYPDIVTLADGTPVIVKTEEKDAFKMKPEQLAASI
                     TARTRALILNSPSNPTGAAYSPDELKAIAQVLLDKDILIITDDIYEKIFYAGFPFANI
                     ANVEPKLKDRVVVVNGVSKTYAMTGWRIGYAAAAEEIASAISKIQSQNTSNPTSIAQK
                     AAVEALSCDQTMVGDMAAEFKKRRDYIVAALNEIPGISCFSPEGAFYVFPNVSGIYGR
                     SCGGKVINGSADFIDYLLDEANVAGVPGAAFGSDSHIRLSYATSLKNIEEGIRRIREA
                     VSKLT"
     gene            complement(19474..19977)
                     /gene="coaD"
                     /locus_tag="PLR13_02620"
     CDS             complement(19474..19977)
                     /gene="coaD"
                     /locus_tag="PLR13_02620"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQC18254.1"
                     /translation="MQEDFTKRIAIYPGSFDPITNGHVDIIKRGKRMFDEIIVLIAYN
                     PNKVYLFNIEERMEMIREIFGETPGIRVDCYSGLLVDYLKMSGANIILRGMRALSDFE
                     YEFQMALMNRRQTKHIETVFLMSGFKWFFTSSKLIKEVASLGGSVKGLVPDNVYQRLT
                     EKYNTRG"
     gene            complement(19977..20534)
                     /gene="rsmD"
                     /locus_tag="PLR13_02625"
     CDS             complement(19977..20534)
                     /gene="rsmD"
                     /locus_tag="PLR13_02625"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQC18255.1"
                     /translation="MRITGGKARGKMLLFPSGSKERPTTDFLREALFNLTGSLNEKTF
                     LDLFAGSGSVGLEAASREARQIVLVENNQKLAGVIRKNIPNGLQDGRLTIMATDVAFA
                     LRDLFRKQCSFDIIFADPPYHRGLVAVTLELLGKYPVLAKGGILVMQHSVKEPIPETV
                     LGIFHRTDQRKYGENALTFFKMERE"
     gene            complement(20571..21353)
                     /gene="pssA"
                     /locus_tag="PLR13_02630"
     CDS             complement(20571..21353)
                     /gene="pssA"
                     /locus_tag="PLR13_02630"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQC18256.1"
                     /translation="MNVENAARKIRMKKGIYLLPNLFTSASLFCGFYSAIASFKEHFV
                     TAAIAILVSVIFDGLDGRVARLTNTTSKFGAEYDSLADVISFGIAPALLAYSWSLSVY
                     GKWGWVVAFLFVLCGALRLARYNIQIGIIESKVFNGLPIPAAASVIATTVIFFDYVGA
                     EGKFHNIFIMVFVIMISLLMVSSVKYYSFKDMSLLVRKPFTIFFWSTVLLIIIVAEPE
                     IMFFVIILGYALSGPVWWLIKFGFGKPKAVPENKNNAANGKP"
     gene            complement(21350..21994)
                     /locus_tag="PLR13_02635"
     CDS             complement(21350..21994)
                     /locus_tag="PLR13_02635"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQC18257.1"
                     /translation="MKHDSIIAAEGYPFVVFSLIVTVFVAFLGGNWWLILFFALVTVF
                     ILCFFRNPERSFPEDEKVLISPADGKIIKIEEVDVNGTLSGKFKKISIFMNVFNAHVN
                     RAPYDGKIEAVHYHPGKFFSANLDKASEDNERNEILIRTPDNRAVWAVQIAGLIARRI
                     VCWVTPGMPIRKGERIGIICFGSRVEVYLPVDSAISVKLKDKVKAGQTVLGYLS"
     gene            22250..23725
                     /locus_tag="PLR13_02640"
     CDS             22250..23725
                     /locus_tag="PLR13_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQC18258.1"
                     /translation="MTRPFKWPMAAGILVTIAFAVLLLIRLDFFQTQTPPVAGIKMVP
                     PQQPQDIWMTIFQNDRKIGFVHRMLTKNENGFAFNENVFMEISTMGMPQALNLATEGQ
                     LNADMTLSSFTFKLNSSLFQFHIRGRVADNKLILYTGAPNRQQKNEMPLQALPHISGN
                     IYDAAFHAGMDKGTTRGFSIFDPSSMSVRTINVTRNADEIIPIMGNRLLLKKYCADFM
                     GAKHCAWLDKDGQVLKETGMLGLSMEKTSRENALAEIDGSAGVDFTQIASIPSNVDIA
                     DPEKLRKLQIRISGITPPLQRQLHLDGDRQHYHDGVLTITREDPADKGPAGGWPADIA
                     VFLKPAALIQSDDPQIKALIGKIVRPEDSAFVKARKIINWVFLNIEKKPALSVPNALE
                     VLKNRSGDCNEHAVLTVALLRAAGIPARSEAGLVYLRGRFYYHAWCVLYINRWITADA
                     VFNQIPADVTHIRLLRGETDQQIRLIGVIGKTNLEVLSQTR"
     gene            23722..24456
                     /locus_tag="PLR13_02645"
     CDS             23722..24456
                     /locus_tag="PLR13_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18259.1"
                     /translation="MITLSELTKDYGTTVAVDKLSLTVNGGEIYGFIGPNGAGKTTTI
                     RMMGGILAPTSGSIVIGGVDMARNPVEGKRMIGFVPDRPFLYEKLTGMEFLEFSANLY
                     NVRRGLFPEKAKRLLQQFALWTWADELIEAYSHGMKQRLIIAAALLHDPQILIIDEPM
                     VGLDPEAVHMVKDILKDLAASQTTIFVSTHTLSIAEDLCHRIGLIHKGTLLAEGSLKE
                     LKQIARLGEARLEEVFLTIIKENTFL"
     gene            24453..26147
                     /locus_tag="PLR13_02650"
     CDS             24453..26147
                     /locus_tag="PLR13_02650"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18260.1"
                     /translation="MKTLLSLIGPRVRSSLNSLTRGNRKNVWSRLVMFGGLGLLFWAG
                     IFMIFYRVLLYFHSVQDFGDILAMKFVSMIILTFFMLLLFSNIINCLSHLYLSQDLPL
                     LHSLPVSANDIFFSRWIISAFDSSWMILAFSLPVFLSYGIIFHTGIFYYAIFTTVMIF
                     MCLIAAALSGILVLFGSKVLPAGRIRTILILLGALMMIVLIFVLRFTRPEQLVNPDSF
                     ASVVLYLNALKTPSSPVLPTTWITDAILAALKNDRATALLNAGLVVTFACMLVFISSM
                     LARNMYFPGFSKSQMTPRRLFAPLRYKGFSWETLLNFLPRESKAFAVKEIRTFFRDSS
                     QWPQLFLMTALIVIYIYNFSVLPLESYPIKTIYLQNVFSFLNIGLAAAVLAAVAARFV
                     FPAVSMESEAFWIVQSAPVSIGNFLWIKFFLYYIPLMMMAEGLVVASNILLNVSDFMM
                     LLSVLTVFFLVPAIVGLATGLGAVYADFKSENPANIVTGFGGLLFMILSFGLIALVII
                     LEAGPVYTIFMADVLGKNLTALKILWAVLSFALAIFLCLLATFYPIHLGRKRLTVK"
     gene            26467..27093
                     /locus_tag="PLR13_02655"
     CDS             26467..27093
                     /locus_tag="PLR13_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC18261.1"
                     /translation="MEKICIVKRRREYTQPEVKVTVTQPAISETAAPEVAVSRVDVKN
                     NAGLSENTARTTKESMTPELTIRSVGMEHEIKIPERKIVEEFVDNASSVIAITMTKEQ
                     SDLLLQSEYIKELLSGKKSDPALDIKVTPEGRLSLNYRFSDSLLLRMIAPNQVCQMLQ
                     VSRSFLQKLVHENKIKSYKLGRMRRFLLEDILEYLSSEVDFMQPSDDK"
     gene            27163..29190
                     /locus_tag="PLR13_02660"
     CDS             27163..29190
                     /locus_tag="PLR13_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18262.1"
                     /translation="MYCDYWNLTKPPFDNVPDASMYVDCHESMENVISETLFAIKEGN
                     ECLTVIVGEVGLGKTLSLRVIIDSLEPEKYKVALITNPALSFVQLLREIIGQITGKQC
                     EVKNKVDLLEIFNRLLFETADQGRKIVIVIDEANVLSPSNLDDLRLLTNMQDDERNLF
                     TLLLVGQMEFAKTLEHPKRANLFQRIGTYGHLEKLPSPEAVRTYIESRLRIAGTDRKI
                     FDDDAIPVIWEFSEHGVPRLINKICKLCIKAGETNEFQTISGDVAQQIADRFQKLSKT
                     SAQKRKAPAAEAQPAAEAPRKQEPERIPKPSRPAFPEKETPAVSAPVRPAPPRMEEPA
                     PMRPRMAPPEQPPARPTAPPVIDEAPPVRPRPVMPEQPPMRRPLHPGMEDLMPPRSRM
                     APPEQPPVSPALPSMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPARPRPVMPEQPP
                     VSPAPPRMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPARPRPVMPEQPPVSPAPPR
                     MEEPAPMRPRMAAPEEMPAYTAPVRQEEPIPVRPQAVPVPPKPSASVVPPPVSAPGNV
                     APPQGVRAADTAQQSYEEQPMPMEAVEEEDFEEVTIGQQSIKLAIPSHVIRQAQSSNK
                     ENKNKLAGYWSAQILKGNPQLTHSSFADPVSLWFEVKNVILKKFNSPAHMQ"
     gene            29268..30089
                     /locus_tag="PLR13_02665"
     CDS             29268..30089
                     /locus_tag="PLR13_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18263.1"
                     /translation="MNIANSKNINKDNYSPSDYENWGFIYLENKEFDQALKCFQKTIE
                     LEPHNPKNYIHLGYVYEKMDMLDSAKKSYEKALEVKSDYIEAILKIGHILEMQGDYHN
                     AVELYTRAIDIAPQAIEPRFCLATLYDRHDMYDEAAEEYENIIEINPKHTKAIHNLGR
                     IYFQDGDYARALKSFQKVLNIEPENTDAWNDLGSVYEITNNLIQAISTYRKALKVNPL
                     HEDTNYNLANAYFSLFLSNPNIVKIDDIVERLQLVLSQNPNNRKVWELLSKIKAS"
     gene            complement(30103..30786)
                     /gene="tsaB"
                     /locus_tag="PLR13_02670"
     CDS             complement(30103..30786)
                     /gene="tsaB"
                     /locus_tag="PLR13_02670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQC18264.1"
                     /translation="MRILAFDTSSQTASVAVLQDDDILYDVIIHTGLNHSETLLPAID
                     QACLRTKIPLPEFDVFACTLGPGSFTGLRIGAGTLKGFMLATGKPAVGVSSLAAIALN
                     AEKTSKKIYALMDAGRGQVYTACFQYGAGGMIEQQGGEQVANPSDIVYDADAVYVGSG
                     VIKYGAAFMPERQNGILSTAKQHCRASAVAVLGKAKYERGERLNAENFVPFYLRSADA
                     DQKKSFFGK"
     gene            complement(30788..31879)
                     /gene="rseP"
                     /locus_tag="PLR13_02675"
     CDS             complement(30788..31879)
                     /gene="rseP"
                     /locus_tag="PLR13_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQC18265.1"
                     /translation="MTTLISFIILLGILIFVHELGHFLVARMSGVGVQKFSLGFGPKI
                     IGRTIGETEYVLSWIPLGGYVKLMGESGTEELSPEDEKRSFARQNVWKRIMIVAAGPA
                     FNFLLAVVIFIAVMMYGLPTLTSDIGEVQKDSAAYQAGMLNGDKIISLDGKKIALWEE
                     IRDVVARAKDRQIEAVVQRGKETVRLQITPKLSKSKNLFGEDVSAYLIGVSPAGQTVI
                     ERKSPVDAVVSSVAKTWEISKLTVISVIKIFEGVISPKTLGGPIFIAQVAGAQVREGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHILFYLIEVVTRREVSAKVREMSQQIGFVV
                     LLMLMLFVIFIDIERLNIKFVNDVVKFFK"
     gene            complement(31876..33039)
                     /locus_tag="PLR13_02680"
     CDS             complement(31876..33039)
                     /locus_tag="PLR13_02680"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQC18266.1"
                     /translation="MKKITLLGSTGSIGLSALDIIEKNPKRFRIAALAAGENIQLLKR
                     QIERFKPEMAAVKNKETAVKLRRALRPGTRIRISYDTEGLQETAACSSADIVISAISG
                     AAGLLPTLAAIEAGKDIALANKETMVMAGRIVTRAAAKKNVRILPVDSEHSAIFQCLE
                     GSRKEDLKRIILTASGGPFLNVAARQLKKVTLRQALRHPRWKMGKKITIDSASMMNKG
                     LEIIEARWLFNTSFDQIDVLVHPQSLVHSMVEFIDGACLAQLGVPDMKIPIAYALTYP
                     ERIANDLKTLDLVQAGRLDFYKPDLKKFPCLKIAREAGVSGGTAPAVLNAADEVAVAA
                     LMQERLSFADLPKIIEKVLDAHDVIPDPSMDDILQADADARQQTEKIIGRITT"
     gene            complement(33040..33858)
                     /locus_tag="PLR13_02685"
     CDS             complement(33040..33858)
                     /locus_tag="PLR13_02685"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQC18267.1"
                     /translation="MAKTPSSLIKRWLTGIVLVPLLLLVIFFGSEAMFAAVVILFICG
                     GVWEYHHIVFGQGFVMEKIESLAFAVLIPLCVLSGNHQYLTALLALAVMLVFVLFIGS
                     IRESSFDFLSVAKVVFGIMYIPFLMSHFIAIRMLDQGVWWIMFVLVLAFAGDTVALYT
                     GKYFGKHKLVPLISPGKTVEGLIGLVVGSTVACLIYSHYLLPDVAVVHVAVMAFVGGM
                     IGQLGDICESAIKRGYGLKDAGSILPGHGGILDRLDCLMFIAPFVYYYRIFVIG"
     gene            complement(33896..34630)
                     /locus_tag="PLR13_02690"
     CDS             complement(33896..34630)
                     /locus_tag="PLR13_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQC18268.1"
                     /translation="MVKIDVNKLPRHIAIIMDGNGRWAKQHAMGRIRGHKKGAEAVRT
                     TVRTCREIGVKYLTLFAFSIENWERPDKEVKALMYLLEEYLNAELDELQKQEIRLTTI
                     GETDRLNETVREKLIETIDRTAQNDKMVLNLALSYGAKDEILAAVKTVVRDAQTGSLD
                     IDRLDKETFSRYLYTAQMPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFTR
                     EDLLKAIASYQKRERRFGLTSEQLQE"
     gene            complement(34809..35366)
                     /gene="frr"
                     /locus_tag="PLR13_02695"
     CDS             complement(34809..35366)
                     /gene="frr"
                     /locus_tag="PLR13_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQC18269.1"
                     /translation="MKETVFQKFKEDMEKTIAGLSKSFSRIRTGRASVSLLDGIKVDY
                     YGAPTPIAQVANVSVPESRLILIVPWDVSIIGAIEKAIQKSDLGLTPANDGKVVRLSI
                     PQLTEERRKEIAKNVKKMAEEGKVKLRNARRDANEELKKLKKDNKISEDELFTAQDEV
                     QKLIDQYIDKTDKILAAKEKEIMEI"
     gene            complement(35369..36094)
                     /gene="pyrH"
                     /locus_tag="PLR13_02700"
     CDS             complement(35369..36094)
                     /gene="pyrH"
                     /locus_tag="PLR13_02700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQC18270.1"
                     /translation="MSTKQKAAYKRVLLKLSGEALMGKQSTGVDPDVANYIADEIKSL
                     SRMGVQLGIVIGGGNIFRGIEASARGMDRTTADYMGMLATVINSLALQSALEERGLPT
                     RVQSSIEMHEIAEPFIQRRAIRHLEKGRIVIFAGGTGNPYFSTDTAAALRAVEIRADV
                     IMKATKVDGVYNADPVRDKKAVKFNKISYIDVLTKNLKVMDSTAISLCRDNLLPIIVF
                     NLQKKGNICKAVCGQKIGTYVGE"
     gene            complement(36094..36699)
                     /gene="tsf"
                     /locus_tag="PLR13_02705"
     CDS             complement(36094..36699)
                     /gene="tsf"
                     /locus_tag="PLR13_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQC18271.1"
                     /translation="MEITSTMVKELRTKTGAGMMDCKQALAESDGDFEKAIDFLRKKG
                     LSAATKRSSKAAKDGTVASYIHMGGKIGVMVEINCETDFVAKTEDFQTMARDIAMHIA
                     ASNPLYVRPEEIPESALEREKEIYRSQLREEKKPEKIWDKIIEGKLKKYYEDVCLTQQ
                     KFIKDTDITVATLINNMIAKTGENMVIRRFARFQLGEEIKQ"
     gene            complement(36749..37564)
                     /gene="rpsB"
                     /locus_tag="PLR13_02710"
     CDS             complement(36749..37564)
                     /gene="rpsB"
                     /locus_tag="PLR13_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQC18272.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGLFQSAYNFVIDTVSEGKEILFVGTKKQSQEAIKEEATRCGMPFVNQRWLGGMLT
                     NFVTIKKSIDRLNSLTDMFNDDSIKAFPKKEITKLQKEKEKMENVLGGIRTIKSLPAA
                     VFIVDPGKEHIAVNEAKKLRIPIVAIVDTNCNPDDIDYVIPGNDDAIRAIKLFTSKFA
                     DAVLEGKKRFEEKLAAESNKEADAAVETKPVEADIPESLEIDDSIDKSETYAE"
     gene            complement(37752..38951)
                     /gene="argJ"
                     /locus_tag="PLR13_02715"
     CDS             complement(37752..38951)
                     /gene="argJ"
                     /locus_tag="PLR13_02715"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HQC18273.1"
                     /translation="MIKNKLKISVPGFLAGGISVGIKKKDQKDLGLIYSTVPARAAAM
                     FTKNTFKAAPLLINEDRVKRGIAQAIITNSGCANAATGKEGIRDALIVSGALSRELAI
                     REELILLGSTGVIGKRLPVKKIEGGIGKLVKGLRETGIEDAEKAMMTTDKYPKIAIRK
                     GVIGAKDITICGIAKGAGMIEPNMATLLTYVMTDALIDYRALNTVFHQAINATFNSIS
                     VDGCMSTNDMAIILANGLAGNNPLEKVQARLQLFRDMLTEVLMELCQAVVKDGEGATK
                     FISIMVKGARSKSDARCAAYAISNSNLVKTACFGEDPNWGRIIAALGASGISMDKEKV
                     VLTIADLAVFSQNTPQEVLMSRLKDAFKKDRIDIEVDLGAGDKSYYVYTSDLSHEYVK
                     INAEYST"
     gene            complement(38970..41498)
                     /gene="secA"
                     /locus_tag="PLR13_02720"
     CDS             complement(38970..41498)
                     /gene="secA"
                     /locus_tag="PLR13_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQC18274.1"
                     /translation="MLDNILKKIFGTKNDRELKRLSLLLREINNLEPAMTALNDEELK
                     AKTPYFREKLKNGFSLDDILTEAFATAREASRRTLGMRPFDVQIIGGIALHEGKIAEM
                     KTGEGKTLAATMPLYLNALEGKGCHVVTVNDYLARRDAEWMGPIYKFLGLTVGVIIHG
                     LDDPERREAYHCDITYGTNNEFGFDYLRDNMKFTLDDYVQREFNYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYKINQIIPRLKKDKDYSIEEKSRTVVLTEDGVAHVEKLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKDGQVVIVDEFTGRVMPGRRYSDG
                     LHQALEAKENVKIERENQTLASITFQNYFRMYKKLAGMTGTADTEAAEFKKIYNLDVL
                     VIPTNMPMIREDHNDLIYKTEREKINAVIGEVKTLNKERRPVLIGTISIEKSEMLSKH
                     LLRAGIKHHVLNAKNHEKEAEIVAQAGQAGMVTISTNMAGRGTDIKLGEGVAELGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDRGSSRFYLSLEDDLLRIFGAERISGIMDKIGIE
                     EDQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNRQRKVIYEQRKKVLRGDN
                     LWADTEEMIEEIIDNILSEFVDEKTKKEDINFKGLDDALLKQFNIRFDLANTDQTGDG
                     QSAIREWIIQGVQKYLRDKEKEFGPELMNYLMKVIMLQAIDVQWKEHLLAMDHLREGI
                     GLRGYAQKDPVREYQREGYDMFMEMIARIKMDVLEKLCLIRIQREEEVEAIRQKQKQD
                     YVMSRGEDTPEKKTVKHEGPKTGRNDPCPCGSGKKYKKCCGASV"
     assembly_gap    41711..41810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41811..42367)
                     /locus_tag="PLR13_02725"
     CDS             complement(<41811..42367)
                     /locus_tag="PLR13_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18275.1"
                     /translation="MSDNFFTFMIIPNRKSGVKKISVPKVAIRNILIGAIVVVLIALF
                     VIYDYASIKRDRAELARLRAQTKEQSRQLQDMGMKIDQFADRLEALRQYDKQIRVLAH
                     ETNLSKKMPLGVGGSEEVRMKDLVDKDHEQIIAGMRRNFEKLHEEANERERSFTELLG
                     YLGQQKAVLASTPSLWPVKGWVTSE"
     gene            42663..43256
                     /locus_tag="PLR13_02730"
     CDS             42663..43256
                     /locus_tag="PLR13_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQC18276.1"
                     /translation="MSRPHQPDGSSQVLLVQRCGFWIFTRTIVYVMEQREGNWSAVSP
                     PMDAVIGRNGFAPPGEKREGDGKTPSGIFPLVMAFGYADSANTKMLYRQIKEDDIWVD
                     DSEAADYNRWTKQSVTQSTSYERMKRDDDLYKYGIVIAHNTNPVVQGRGSAIFVHVWK
                     DKSVPTAGCVAVSEENILRLLHWLDPAAAPLIIMGLK"
     gene            43277..44044
                     /locus_tag="PLR13_02735"
     CDS             43277..44044
                     /locus_tag="PLR13_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQC18277.1"
                     /translation="MKIRFLLAVTVSFSFFYFASAAHCSDLNKKPDRFVDIQTVIPGA
                     LLDIRYHGAHNFLGERVDGYLAPKCYLTKEAADALALVQKDLEAYSLTLKIYDCYRPQ
                     RAVHHFVRWAKDIENTKTRKEFYPTVDKRNLFTDGFIADRSSHSRGSTVDLTIVLLSA
                     PPQPDYTPGQTLSECYLPSGKRFADNSIDMGTGFDCFHELSHTANAKIGEQQKINRLL
                     LKSLMEKHGFKNYDLEWWHYTLKNEPYPDTYFDFIIE"
     gene            44025..45281
                     /locus_tag="PLR13_02740"
     CDS             44025..45281
                     /locus_tag="PLR13_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQC18278.1"
                     /translation="MISLSSENMNPTQPSFLKSYGFSILLIASIVIGAVLGFVFKADA
                     VRLKPLGDIFLNLIFTAIVPLVFFSIASAVAGMQNIRRLGKIMSAMIFVFIATGLIAS
                     AVMIAAVIFFPPAAGLNIGLGNGVDVEHLKASDQIVKAFTVGDFTELLSKKNMLALIV
                     FSVLTGLAASVTGERGKPFANFLNSGSAVMMKVISFIMYYAPVGLCAYFAYLVGVFGP
                     QLLDSYARAMMVYYPTAFLYFFIAFSLYAFIAAGIDGMRRFWKNIIPPSLIALATGSS
                     MAAIPANLEAADKNGVPRDIREIVIPVGATMHMEGSCLAAVLKIAFLFGLFNMPFSGA
                     ETLLTAFGIALLTGVVVSGIPGGGTIGELLIISFYGFPMEAFPIITMIGTLVDAPATM
                     LNAVGDNVSGMLVARILDKKNPVAPD"
     gene            45511..45873
                     /locus_tag="PLR13_02745"
     CDS             45511..45873
                     /locus_tag="PLR13_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18279.1"
                     /translation="MTNDKEYRLSASINDGIIEIILSGTVTRTNVSRLQKEIDSLRNE
                     KGSRLLIDVRTLNEGSGYHDVLYYIRRPDKATGKTAILDVPGNDRLETFLKTATLNTH
                     LEARYFSNLDLARNWLKT"
     gene            45971..46261
                     /locus_tag="PLR13_02750"
     CDS             45971..46261
                     /locus_tag="PLR13_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18280.1"
                     /translation="MMRRCHKCLNEISDNVYTGRQSQCPVCSADLHCCLNCSFYDAGA
                     YNDCREPQAERVLDKARSNFCDFFRFRQTETLSDAPSQNTPGDKLEALFKKS"
     gene            complement(46273..47508)
                     /locus_tag="PLR13_02755"
     CDS             complement(46273..47508)
                     /locus_tag="PLR13_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQC18281.1"
                     /translation="MKVDTMRRIDFYAGVPLCFFGSLFYKAVKWLSPRTKKDPKNVLF
                     IELSEMGSAILVDPAMRKLRDTTGAQLHFAIFKKNKPSLDLLQTVSPDNIYAIREDRL
                     SSFLMDTFKFLHWTRKRKIDTVIDLELFSRFTALLTGFSGASNKAGFHAYFNEGLYRG
                     DFLSHKVAYNPHMHIAKNFIALVNALLSPKEEIPYSKTRIQDAEISMAKVHITDTQKK
                     TVLDIIRGCFDAFDPSRHKIVLINPNASEMLVQRRWPQAYYGHLIKMILGQCSHAVVL
                     ITGDPREREEAERLKEQVQDERCVNFAGKVSFAQLPQLYSVSEFMVTNDSGPPHFAAI
                     TEMPTYVIFGPETPALYGSLGRTTPIYAGLACSPCVSASNHRKTACTDNVCLKVITSS
                     QVFETIRPVLDRINAETLL"
     gene            47812..48927
                     /gene="lptF"
                     /locus_tag="PLR13_02760"
     CDS             47812..48927
                     /gene="lptF"
                     /locus_tag="PLR13_02760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HQC18282.1"
                     /translation="MRKIIDRYIFKEIAFPFILILCVLTFVLLMGKILQIMDLFINKG
                     VSIFSIAKIFIFMLPYFMLFTIPIALLISILIAIGRLSSDNEITALKTSGVSMARIYY
                     PVAVASLLAFVFTLLVSYFVAPQSNFATKRLLFDIAQQNASVGIKEKVFNDSFKGFII
                     YTNKISEGNHALEEVIVSDKRTIGEQNTIIAPRAFLVSDPDTMRVKLRMENGSIHTVS
                     SDLKNYRKIDFQSYEINLDLYAALAEFDKASKAAKDMTMTELLQNMKKPGMSEKDVRE
                     LSIEAHKKFSIPLACIFFGLLALPLGMRSQRSVKARGFAVGLITVCLYYLLRIGGEAL
                     AETGYIPVAVGVWIPNISFAFIGIFMFYITSKEIYLF"
     gene            48979..50058
                     /gene="lptG"
                     /locus_tag="PLR13_02765"
     CDS             48979..50058
                     /gene="lptG"
                     /locus_tag="PLR13_02765"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HQC18283.1"
                     /translation="MKLLDKYILKEFLKFFTITFLAFIALFLIVDFFERIRMFLSHQA
                     TAAQMASYFIFSVPMIISYVLPPSILLATLMTFSSLTKFSEITAMKANGINIFRMALP
                     IVMFASFTAVFYFYFTELITPAAVQKTTYIKNIEVKKQKTPGFFKQNEIWYRSDNAIY
                     NFKMFDIGKNILHGVMITYLNKDFTLISRIDARRAEWKNGRWVFHDLLTTRFDDNRAP
                     VLERSKEKIIPLPEKPEDFKVMQKDVEKMGYFELRRYVQKIQSEGFDARKYNVDLHGK
                     TAFPFVILIMVLIAVPFALRSERSGGVMQSIGIGIFIGFSYWIVHAFCMSLGKSEMLP
                     VVLAAWAANILFGAAAAFLLYKVNT"
     gene            complement(50093..51517)
                     /gene="ahcY"
                     /locus_tag="PLR13_02770"
     CDS             complement(50093..51517)
                     /gene="ahcY"
                     /locus_tag="PLR13_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQC18284.1"
                     /translation="MAFLEIDPALKYKVADIALADWGRKEIKLAENEMPGLMALRKKY
                     GQKKPLKGMKIMGSLHMTIQTAMLIETLKTLGAEIRWASCNIFSTQDHAAAAIAKKGS
                     AAVFAWKGESLEEYWWCTEQALTWPDGSGPHLIVDDGGDATLFVHQGVKVERDPALLK
                     QKIDNKEFKIILDRLEKSFRRNPKKWQKIASTIKGVSEETTTGVHRLYQMQAAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRGTDIMISGKIVVVCGYGDVGKGCAQSMR
                     GFGARVIITEIDPICALQAAMEGYEVRTMEEVAPIGDIFVSATGCCDVITGRHMEMMK
                     NEAVVCNIGHFDSEVDMHYLETSKVCRKETIKPQVDKWTLKSGRSIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQCLAQIELATKKHKIGVYTLPKVLDEEVARLHLERLDARLSK
                     LTKKQADYLGITVDGPFKPDHYRY"
     gene            complement(51713..52069)
                     /locus_tag="PLR13_02775"
     CDS             complement(51713..52069)
                     /locus_tag="PLR13_02775"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQC18285.1"
                     /translation="MQRFMLKSKIHRATVTDADLHYEGSISIDEKLLEAASILPYEKV
                     AIYDVDNGERFTTYAIKGKRNSGIICLNGAAARKVAKGDLIIIASYVVVEEAETKNWK
                     PTCVFVDGKNKIKKKH"
     gene            complement(52094..52957)
                     /gene="panC"
                     /locus_tag="PLR13_02780"
     CDS             complement(52094..52957)
                     /gene="panC"
                     /locus_tag="PLR13_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQC18286.1"
                     /translation="MNVVKSVQKMQSFSENLRLEGKKIACVPTMGYFHEGHLALMREA
                     RKQADCVVVSIYVNPTQFGPQEDFSKYPRNFDRDMKMAQNVGVDVIFYPSNRAMYPPH
                     YQTYVAVEKVTQNLCGASRPGHFRGVTTVCNKLFNIIKPHVAVFGKKDFQQFVVVQRM
                     VRDLNMDLEIIGYPTVREADGLAMSSRNKYLSAEQRTSALVLFKSLQLAQKLYAKKET
                     SPRIIIDAVKKMIQKTPLTQIDYVKICDTTRLQDIAKITDQTVIALAVRIGSTRLIDN
                     HVFGEALKISY"
     gene            complement(53145..53546)
                     /locus_tag="PLR13_02785"
     CDS             complement(53145..53546)
                     /locus_tag="PLR13_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQC18287.1"
                     /translation="MGRYKVKKGENLSTIACRFSVSVSELKAINNLSSGQIYAGQRLK
                     IPQKEDGKDSGNEDSEKNLRTSAQKSKIDKKILSAKDINQLGPDKYIVTKNDNLHTIA
                     KKNNTNAARIMELNGLSVDAKIVPGQILVVQ"
     gene            complement(53552..55072)
                     /locus_tag="PLR13_02790"
     CDS             complement(53552..55072)
                     /locus_tag="PLR13_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQC18288.1"
                     /translation="MTGLTRNRLIGLIALICLFIVPLIGYAENSIDKKSPESSGDITK
                     QKIDQKIKLAALNNDDESSLFDFPQENDPLADVQEDDEKQDMMENALELLGEADKLWK
                     NGDIEETLDTLDEAYSLILNANGDSAIAQEKDDLRLLISQRILAVYSSKRDVLQGKNG
                     EIPLLMNGDVQKEITSFQTVERDSFLAAYERSGMYRSEIVKELKRSGIPEEFFWLPLV
                     ESLFKINAYSSARALGLWQFIPSTGYKFGLNRDEWVDERMDVQKSTKAAIAYLKELHG
                     MFGDWLTALAAYNCGEGRVLRVISRQRLNYLDSFWDLYRQLPNETARYVPRFLATLHI
                     VNNPQKYGFDLSHTARPIEFETVRVNRIMKFNDIAERIDTTEDILCLLNPELRHKMTP
                     DREYDLKVPGGMLDKFSFVYHEIPSSEKPSIASVQTYVKASDRTRRTYIKHRVKRGET
                     LYSISRKYKVSISSIRSLNKLSPKKKLIQGRRLLIPVASAKRHAKGLTVKKKFKKK"
     gene            complement(55242..56349)
                     /gene="prfB"
                     /locus_tag="PLR13_02795"
     CDS             complement(join(55242..56276,56278..56349))
                     /gene="prfB"
                     /locus_tag="PLR13_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQC18289.1"
                     /translation="MTTENILETISDLKRRIQHIGECLDVPELKMKIKDLEELSLKKD
                     FWNDQDYARSVLQKKTNLSEKVERWDRFSKDISDIENLAEMAVREKDEHVLQDIDEEL
                     EQLNAAVAQEELKMMLNGEQDAMNAIVSIHAGAGGTEAQDWTEMLLRMYLRWAEKKKF
                     AARIIDYLPGDEAGVKSVSFTLEGECSYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEVDDDIKIEINDDDLRIDTFRAGGKGGQHVNKTDSAVRITHVPTGIVVQCQNE
                     RSQHQNKAMAMKFLKSRLYERELQLKNQKIDEENKQKKDIAWGSQIRSYVLHPYKLVK
                     DHRTNVECGNAGKVLDGDIDEFIQAYLMMTTTTS"
     gene            complement(56346..57908)
                     /gene="lnt"
                     /locus_tag="PLR13_02800"
     CDS             complement(56346..57908)
                     /gene="lnt"
                     /locus_tag="PLR13_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQC18290.1"
                     /translation="MKNNFPEVSLADISRNLFRKNIFFAALSGILLFLSFPKYGSGVL
                     AWVALVPLFYALKNAATIRHGLLLGFITGIISYVGIIYWIAHVIVNYGHLPLHIGIAL
                     MLLLAAYLSLYLSFFAGCLVSFREKVPFYIIGPALWVSLEFCKSFILTGFPWENLGYS
                     QYGNTYLIQVADIFGVPGLSFLIVLVNAVLLDVIHRRTKKEYVAAGMVFVLIAGIYVY
                     GFFRLREIDATVTNAPGIDVSLIQGNIDQAVKWDQDYKQETLDIYERLSLKNKPGRGS
                     LIIWPETAVPFKFQNINRFRRQIIDLSLKTQSWLLFGSVSYEPGAYGNDFFNSAYLLS
                     PSGDVRGRYDKVHLVPYGEYVPFRDLMPFVKGLTSGIGDFSSGAGFDPLSLNDKKIGV
                     LICYEGILPFASRAYKNKAAELLVNITNDAWFGATSAPFQHFSMAVFRAVEMRLYLVR
                     AANTGISGIIDPAGRIIAKTGIFQEEALKGHAKFVTIPTVYERVGDIFIIGCFMMVSI
                     CFIFSLRRRSQK"
     gene            complement(57898..58731)
                     /locus_tag="PLR13_02805"
     CDS             complement(57898..58731)
                     /locus_tag="PLR13_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQC18291.1"
                     /translation="MNIKKNISKLFFWREQKYIKNKINAMLSVAQKSGLVDASSREMI
                     ENILEFSHTLVREIMIPRTDIVAVSTEAAIDDIIHEVIASRHTRIPVYRDTIDNIIGI
                     LNVKDILMFWSKPMTKEDLIACLSTPHYIPETKNTHLLFYELKENKKHMAIVIDEYGG
                     TAGLVTLEDMLEEIVGEIRDEHDGNVTDDRIIQSPDGSLIFDGRMEIEKIEENLNIDL
                     KKGRYETLGGLILDAIKRIPVSGEKFQIEGLDVTIDKADERSIKKVKIKKIETDNDDE
                     K"
     gene            complement(58748..59620)
                     /locus_tag="PLR13_02810"
     CDS             complement(58748..59620)
                     /locus_tag="PLR13_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18292.1"
                     /translation="MDYEGFIIRPPSEAYSLLLQVTTGCSHNKCTFCGTYRQKKFKIK
                     PIERIKKDLDEARQYEDVDRVFLCDGDALIIPQPKLKEILELINENLSDIERIGTYAN
                     AKSILRKSVEELKELKSLGLKIVYLGVETGNAELLKKIHKGVTCEQMIQAGRRIKEAG
                     IILSVTVLLGLGGREKSIDHALDTAKILTDMDPDYVGALTLMLIPETEMYEDYLAGRF
                     VLPDQFGFIRELYLMIEKSSFTDCYFTSNHASNYLPVKAHLPREKEKNLKMIGSVIHE
                     KDLSRLRPEYLRGL"
     gene            complement(59941..60813)
                     /locus_tag="PLR13_02815"
     CDS             complement(59941..60813)
                     /locus_tag="PLR13_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQC18293.1"
                     /translation="MKYWKITSVISAAICFLITHQAWAAREWVYLESNSSRESYYDKN
                     SIVKVDENVFRLWMKQVLSENGKIKTISKFRNTNKPDNSLDLISYILRQMDVDCRNNQ
                     YKDSALIFYDEKSNVLYSSPKGAIGKWDDIVEGSFAYQLKNVICKGPNLAEKPSAILS
                     ADPAEKPAEAGTPPQSLPAGTSDEDIRQVVSKWLAAWQSGDIATYRSCYDEDFKSKNM
                     TLEKWIAYKTGVRQKSTDIKIGIDDLKITADGDHAEAVFIQTYSSSLLNDKGKKTLKL
                     KKVGNEWKIYQEIM"
     gene            complement(61025..>62347)
                     /locus_tag="PLR13_02820"
     CDS             complement(61025..>62347)
                     /locus_tag="PLR13_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQC18294.1"
                     /translation="VVILLFAGTTGVRGETLRDSVKSVLQNNPDVTSVAYNRLARDQE
                     VRQAMADFFPRIETTLSDGYIDQHHPYLLDDEFHPRSATVRLNQNIFRGGATFAETKR
                     QKSRVISAAYQLQGVSENTALLACRVYLNYLRAIEQDNLAKENLLIHERFYDQIKLRS
                     QAGVDRRVDLDQITARLALAKSNLIVTQANIENAKTDYQAVMGYLPVNLVKPEPAYAE
                     IPATLQDAERIALASHPTLKSAKADIEARNYQHKTARALAYPTVDVGAGYTWGEDLNR
                     PAASYDYEDYLQLNATLSFNIFNGFINQARIKETSYLINEAEEIAKKTELQTIQSVRL
                     SYESYLADQRRVRELEQYVDSTDKAAEAFISQWSIGRRSLFDVLDSSAEKISAKSDLI
                     NAQYDKMYSAYRILSGLGNLVHTLGLQWPEESRVDTGAGIVDMSFLEK"
BASE COUNT        14759 a        16233 c        14936 g        16319 t
ORIGIN      
        1 ttcgcttccc ctgtcattcc cgcgtgcccc acacaactgt cattcccgca tgccccacac
       61 aactgtcatt cccgcgtgcc ccacacaact gtcattcccg cgtgcccctg gcgggaatcc
      121 aggtatgaaa tatgagtcac ctcctcgtct tcccggcgaa agccgggaac caggaatgaa
      181 tactggatgc cggtctgcac cgacatgacg aaaaaagaga caaaccggat accggccttc
      241 gccggtatga ccgacagggt tatggatgcc ggtctgcacc ggcatgacaa actttggatc
      301 cattccggag ggagcctggc ggaccatggt gccgccgtca acatccgcta cacttcgggg
      361 ataatttgca atggcaaacc cattggatag ttcgtcctgc acatttcagt ggagttgccg
      421 tgcaggggct ttatattagg cattgcaata tccggctcat ttcattagaa tcatttttgt
      481 cggcagtcgg caaacgttaa ccattttttc aatccatatg ccggagagcg atatggacaa
      541 caccaccccg tacgatatcc aatatctgtt tgagccgaaa agtatcgccg tgatcggcgc
      601 ttcccagaac aaaaagaaaa tcggctatgc cgttttcaac aatattgttt ccggcggata
      661 ccagggaaat gttttccccg tcagtcccaa aggcggtgat gttgacgggc ataagatttt
      721 ttccgatatt ctggacattg acgaggagat cgactgcgcg tctatagtga ttcccgccga
      781 tctggtcaag gatgcggtaa aaaaatgcgc cctgaaaaag gtcaagttcc tgcagatcat
      841 cacgtcggga ttttcagaag tcggaaatgt ccatgaggag aaagagatat ttgatatcgc
      901 gtcgcaggcg ggaacgcgta tcgtggggcc gaatatgttc ggcctgtact cccgtgccgc
      961 gtcgctgaac tcgacgttct ccgcagcaaa gattagtccg gggcatgtcg ccatcctgac
     1021 gcaaagcggc gcgctgggaa tcgccatgat ttcgaaaaca gccgtcgcca acctgggcct
     1081 ttccgccatt atttccatcg gcaacaagtg cgacgtggat gaagccgatc tgctggccta
     1141 tctgattcaa aacgacgata caaaagtcat ctttatctat atcgagggcg ttaaaaaagg
     1201 agaacggctg attgaagccc tcaaggttgc caccggaaag aagccggtga tcgttatcaa
     1261 atccggcaaa tcgaagagag gcgcgcaggc tgcggcttcg catacgggat cgctggcggg
     1321 ctcggatgac atattcgacg ccatcatgaa acaatgcggc gtgctccggg cggaatgcct
     1381 cgaggatgcg tttaactggt gcaagtttat gatttccggt tcggcgcccc ggacccgcaa
     1441 caccgtcatc gtgaccaacg ggggcggcgt cggggtgatg gcaaccgacg cctgtgaaaa
     1501 gtacggcgtc tctctgtatg atgatcaggc cgcgcttaga gggctttacg aggccgtcac
     1561 gccatcgttc ggatcaacga aaaaccccat cgatcttacc ggcgccgccg gcggaaccga
     1621 atatacacgc gcgttcagcg taaccgccga atctgaagat atcggttcga ccatcgggct
     1681 ttattgcgaa acggcgactt tcgattccgg aaatctggcg caaatgatcc aggagaccta
     1741 cgcccgccat cagaaaaacg gcaaaccgat cagttacgcc ctgatcggcg gcaaacaggt
     1801 ggaaaacgcg atcaccgctt tgatcgcgga gcgtattccc gtattcaacg atgtggaaca
     1861 ggccgtctcg tgcatgggcg ctttctaccg gtatcacaac tatcttgacg aggtttcccg
     1921 caaaattgac gagtatgctg tcgacaccgc cgcgatcaat gccgttatcg accgggccgc
     1981 cggagcggga agaacctttc tgctggccaa tgaaggcgcg gcggttttgc gcgccgcgga
     2041 tattgcgatg ccgcaaagta aaatagcgcg cagcattcag gaagccgtgc agtttgccgg
     2101 agaaatcggt tatccggtcg tcctgaaggt ggtctccaaa gatattctgc ataagagcga
     2161 tgcgggcggc gttttgcttg atctggatga tgaaaaggaa gtgatcgtcg gttatgaagc
     2221 gatcatgcat cactgcagga tgtataaacc caacgccgtt atcgagggta ttgaagtcgc
     2281 ggaaatggtc aagaaaggca tcgagctgat tatcggcgcg agaagagatc cctccttcgg
     2341 accggtcatt atgtgcgggc tcggaggcat ttacgtggaa attatgaagg acatttcgtt
     2401 tcgggcggtt cccatcaatc gcagcatcgc cctttccatg ctggaagaaa tcagatctta
     2461 tccgctgctt ctcggagccc ggggagaaga gaagaaggat attgaatctg tcatcgatac
     2521 gatcattaaa atcggttcga ttatcaggac gtgtgaacgg atcactgata ttgaagtcaa
     2581 tcccatcgag gtctatgaag ccaaaagcgg catcaaggct gtggacgcgc ggattttgat
     2641 aagcagaatg aaggaggata cgcaatgaaa aagatcgttg tttcttcaat gagacgcaat
     2701 tcgggaaaaa caagcatcat cgcgggaatc gtctcgctga tgcacgataa aaaattcgct
     2761 tacgccaagc cgctgggcga caggctgatc tatcgcaaaa aaagaagctg ggattacgat
     2821 gcgagcctga tggtcaatct tctcggacgc cagggagaaa tggaaagcca ctgcgaaaaa
     2881 atcacgctcg ggttcgatca ttcccggctg aaatatatgt atgataaaga aggcatcgaa
     2941 agagcccttg ccgagattgt gacggatatc ggcgccgaga acgacgtgct cttcatcgaa
     3001 agcgggaaag atctgaccgc cggcgcttat ctcaatctgg atgcggtgtc catgaccaag
     3061 tccattgacg gcgtactcgt gattgtcgtg agcggcgatg atgattgcgt gctcgacgat
     3121 atgaggttca ttgaaaaata tgtcaaactg gaaggcgtcc atttcggcgg cgttattatc
     3181 aacaagatca gcgacattga agagtttgaa gcgtcctgca caccggtcat ccgggagatg
     3241 ggcctcgatg tgatcggcgc gattccctat gaagagccct taacgtactt tccgcttgat
     3301 tttctcgccg acaagcttct ggccagagtg ctcgcgggcg aagagcattt gaaaaatatc
     3361 gccaggacga ttctcattgg aacctctgcg acgtttgttg ccgacaaaga acagattccc
     3421 gcccgtccgg aactcagcgg caccggccaa ctgatgatta ccggcggcga ccgaagcgat
     3481 ctgattcttg ccgcgcttga aagagatacc gcgggcatca tcctgacgaa tgacattgtt
     3541 ccgcatcaga acatcatttc cagggccaac gaacggggca ttcctgttct gcttgtggga
     3601 acggacacgt tccagaccgc caggacgatt gacgatatgg aacccctgct cagaaagcag
     3661 gacaccggca agatcagtct tttatcgcaa ttgattgaaa agcatacgaa catggcggag
     3721 tttctgaaat aacgggatga cctccggggt atagttgata gttcatagtt catagttgat
     3781 ggtgaaaaaa ggttcagggt tcaacggttg cggactgtgt cacttcgaac gtatgtgaga
     3841 agtcttattt ttcttcacta ttcacttttc acctttcaca tttttgtgcg aagccgatgg
     3901 atacaaaaaa acaagatttt tcttcgttcg ttcttagcaa atctcactag aaggctggtt
     3961 gacaggggca gcggtttaac ttataatact cgaaaaacat cgggataccg tcgtttttct
     4021 tttaatttaa accggggcta cagactgcca tggaacctga aattttcgaa gaatatcttg
     4081 ccggcttcaa gaaagcctat cccaaaaaat tcgtttcgga agatgagatt ttcagccata
     4141 tccgccgcgg agatcatatt tttgtcgcat ccggctgcgg cgaacctcaa tacctcgtca
     4201 aggcgatgat ccgttatgtg gaatcccacc cgaaagcttt ttttgacgcg gaaattatgc
     4261 atgtttactc gctgggactc gctccttaca ccgagcccaa atacaaagcg aatttccgcc
     4321 ataattcttt tttcatcggg aacagcacac ggagctccat caacgccggc atggccgact
     4381 atacgcccat ttccctttcg caggttccct ctcttttagc cagcggcgtt gtccagattg
     4441 acgttgctct gatacaaacc tccatgccgg atgaacatca ttacttaagc ctcggcgtca
     4501 gtgtggacgt ggtcaaagcc gcaacggaaa aggcaaaaat tgttatcgct caaatcaatc
     4561 ccaatatgcc ccgcattcac ggcgacggat tcatccacat cagggacgtt gattttatta
     4621 ttccttacga tgagccgctg ctggaagtat ccagtcctaa aacaggcgac gtcattataa
     4681 ccatcggaaa aaacgttgcc cgtctggtgc aggacggcga tacgattcag gtcggctggg
     4741 gcagtctgcc caacgccgtg atgttctgtt tatccaacaa aaaaaatctg ggcgttcaca
     4801 cggaactttt gagcgacggt ctggtggatt taattaaagc cggcgtgatc aataattcca
     4861 gaaaaaccat cgatcacggc aaaacggttg cgtctttctg catgggcaac aaatccacgt
     4921 acgcctttct ggataataat ccttccatat cgctgcggac gctggattat acgaacaatc
     4981 cggtgatgat gtcccgcatc gacaacatgg tggcgatcaa cagcgccctg gaaatcgatc
     5041 tgagcggcca ggcaacgtcg gaatccatcg gcggcatttt ttacagcggt atcggcggcc
     5101 atcaggattt tatgcggggc gcgctgtttg cccgaaacgg caaatccatt ctggcgctga
     5161 aatccacgtc caagaacgat acggtttccc gcatcgttcc ggcgctcaag gaacaggccg
     5221 gggtcacgct gaaccgcggc gatgtgcgct atgtggtcac cgaatacggc atcgcctatc
     5281 tgcacggcaa aaatatccgc gaacgcgcca tgtcgctgat tgccatcgcc catccgaaat
     5341 tccggccgtg gctgatcgat gaggcgaaaa aacgcggcct gattttcaag gaccaggcgt
     5401 ttattcccgg caagcgcggc gaatatcccg aggacctgga gacgtatctc accaccaaga
     5461 ccggcctgca ggttttcatc agaccggtta aaatcagcga cgagagtctt ttgaaggatt
     5521 tcttttattc gctgtcggat aaaagcatcg aacggcgttt tatttcgacg cgcaaggata
     5581 tgccgcatga gcggctgcag aatttcgtca ttatcgatta caccaaacag attgtgctgg
     5641 tggcgatcaa aggcgatgtc gataattcgt atatctccgc cgtcggccaa tacggaatcg
     5701 acgacgccac gcataccgcc gaagtcgctt tcgctgtgcg cgacagcgag cagaacaacg
     5761 gcatcggatt cgagttgctc aaatacctga cgtatctggc caaacggcag ggcttgctcg
     5821 ggttcaccgc ggaagtcctc gtcgagaaca agccgatgct gcatgtattt gaaaagggcg
     5881 gtttcgatat caagaagaaa attgacagcg gggtttacga gctgacgctg aaattcaaat
     5941 tataatttac caaaggagtt gaccgtttca tgaatcccca tgttttccga gaatacgatg
     6001 tgcgcggtgt tgtcgataag gacttaaacg aagattttgt gttccatctg ggacgcgcca
     6061 tcggcacgta cgccagggcg aaaagaatca ggaacatgac cgtcggacgc gactgccgcc
     6121 tgagctccga gagctatcat cggtttctga gccgcggcct caacgccgcc ggcattcatg
     6181 tgatcgatat cggcatgtgc gccacgccca tgctttattt ttccatccgt cacctgcgcg
     6241 tcggcggcgg cgtgatgatc acgggcagcc ataatccgcc ggaattcaac ggcttcaaaa
     6301 tctgcatcgg ccacagcacg attttcgggc cgcagatcca ggacctgcgc aaaatcatgg
     6361 aaagcggccg ctatgcgaaa ggcgccgggc gctgccggga aaaggatatt gccaaagagt
     6421 acgaaaatta tcttttcaaa cgcattaaaa ttcaacgaag gatcaaggtg gtggtggacg
     6481 gcggaaacgg cgtcggcgga tatttcgctg tgccgctgct caaacggttc ggctgcgaag
     6541 tgattcccgt acactgcgaa ccggacggaa cgtttcccaa tcattttccg gatccgacgg
     6601 tggaggaaaa cctgaccgat ctgatccggc tggtgcgcaa aaacaaggcg gatctgggca
     6661 tttcgttcga cggcgacgcg gatcgtctgg gcgtcgtcaa cgacaagggc gagattatct
     6721 ggggcgacaa actgctgctg ctgttcgccc ggcatattct gaagaatcat ccccgcgctg
     6781 cgattatcgg agaggtcaag tgttcacagg ttctttttga cgatatcgaa aagcattccg
     6841 gaaaggccat catgtggaag gcgggacatt ctctgatcaa ggcgaaaatg aaagaggtca
     6901 aagcggtgct gggcggagag atgagcggcc atttcttttt cgccgaccgt tatttcggat
     6961 acgacgacgg gatctatgcc gcgctgcgcc tgctggaaat tctgtcccgg acgggcaaaa
     7021 aactcagcgg tctctttgcc gatgtgccga aaacattcgc cacgccggaa atcagagtgg
     7081 attgcacgga cagcaaaaaa gcaaaggtgg tcgagggcat caaacagcat ttcaaaaata
     7141 cgcccggcat tgtggacatc gacggcatcc ggattccgtt tgaaaacggc tgggcgctgg
     7201 tgcgctcttc caatacgcag ccggtgattg ttctgcgctt cgaggccgcc tctgtaaaga
     7261 cgctcaacaa aattcgcgga gaggtggagg cgctgctcca tgtgtagcgg cggctgaacc
     7321 gaataacggg tgtcgactca aaataatcta tggcggatgg gccggctacg ccgtgtgtaa
     7381 ttctctgaca cggcgaacca gcaatcggca gcagtttaaa atattgtccg gagaataaac
     7441 ggccagttcg tccgtatagt aaaatccgaa aaggctgcgg gcgaggtctt catcatccat
     7501 cgcgcaatcc tcgatgtatc ggcggacatc ctgtgcggac tttcgcgata aacaaaatat
     7561 gtctgcaatc gctccggatg tggaaaaaaa tccgttgtgc gcaagcccca ttcttttcgg
     7621 ccggaagctc tccagacgat cgatggttgc catgtaatcg ttataaccgg taaaaaacgt
     7681 cgggaaaaag ccgcgtccgg gataatggtt gcccaggctg tccgatacca gaagggtttt
     7741 tgtgccggca atccaggcga gcaggtttcc cggggaatgg cctttgacag aaagaaagtt
     7801 cacggacatc ccgcccagat caagggcttc cccgtcggta aggattctgc atccggaaag
     7861 cgaaggcggc attgtgacga cggcggtttg gctgaacagg ccgcgggccg ccagcgcccg
     7921 gaacatgtgc cggtcttcgg caataattgc cggcgcggct ttcggatgcg cgacaaacga
     7981 tgcggcgccc gccgcaatca taacaacagc gccgggaaac gccccgcgca gatggtccag
     8041 tccggtgatg tgatcgctgt gcggatgtgt aacgacaaga taatccggcc ggacatccag
     8101 cgcctcaagc tgcgaaagca ccaggtccgc cgtcgcggat atgccggtct ccaccagcgc
     8161 gcgttgtttg gcgcccttca gcagataaag atgaaaatag ccgttgccca gtatccagag
     8221 gctgtcattg actttaaacg gatagagtgt gccggacatg gttatggggg ccgcatggaa
     8281 agagcgctcg cgcctcgccg ccggcgttat cgccggcgac aaagcaaaca attcgatctg
     8341 tgtgcggaag caatcccttt ctttttttag tttttctccc cgactttaga aaacattcac
     8401 accgtcaaac tggggattga tggcgtactt gccctgtttg ttgatgaacc cgaccttgac
     8461 cttgccgcgt ccgatgccga ccggcgccaa accgttttca taccaaaacg cgatatcgaa
     8521 ctgcggctcg atgacgtacc ggccgctttt gtcgatgaaa ccgtacttcc ccttgctctt
     8581 caccgaggcc aggccttcgg aaaaccagaa agcgaaatca tactgcggtt cgatgatgaa
     8641 tttgccggtt ttgtcgatat agccgtattt atttccgttt ttgaccgtag ccattccttc
     8701 ggaaaaccaa aaagcaagat catactgcgg ttcgatgatg aatttgccgg ttttgtcgat
     8761 atagccgtat ttgccgtcga ttttgactgc cgcgaggcct tccgaaaacc agcccgcatc
     8821 ttcaaaccgg ggcttgatga tgtatttccc gcttttgtcg atgaacccga ccttggcctt
     8881 gactttctgc ccgtcgattt ttcccaccgc agtaaggcct tctgaaaacc acccggcatc
     8941 atcaaattgc ggattgatga catacttgcc ctgtttgttg atgaatccga ccttgacctt
     9001 gccgccgccc aggccgacgg atgccagtcc gtcatggaac agaaaagcga catcaaactg
     9061 cggattgatg gcgtattccc cggtcttgtc gatgaatccc caccggtcgc cgattttcac
     9121 ggcggcgaat ccgtcggaaa aacgccccgc tccgtcaaac tggggttgga tgacgaattt
     9181 gcccgtcgtg tcgataaagc cccagctgtt tccctgcttc acgggaaagg agatgatctc
     9241 ctcgcttaac attttttcct tgaattttga aaaattatcg caggcggcaa cactgaaaat
     9301 aaaactcaaa cttaaaatga tcacgcataa ttttctgaaa cgctccattg caaaccctcc
     9361 cccatgtttt tgagctttcg tgaaacagct tttcaaaaag tttttatgaa tatggcgcgt
     9421 atcttaatcc atattggatt attttccaat caattatttt tacgcttttc catgcgacat
     9481 gatatgcgtt ttgcaacgtt tcatttttca ggatgatgca acggccggcc gcaggcgttg
     9541 cccgagcgga ctgtgcaaaa taaattaaaa aagattttac accgccggag ttttgaatgg
     9601 tcggggcgga gtgatttgaa cactcgacat cctgctccca aagcaggcgc gctaccaggc
     9661 tgcgctacgc cccggtttta ttttttcatt ttccgtccga gggactgttt cagtttcaag
     9721 aaggattgtc cccggtgact gattctgttt ttgatttccg gcggcagttc cgcggcggtt
     9781 ttgttcagtt ccggcacgta aaaaaccggg tcgtagccga aaccgttgtc gccgcggcgg
     9841 tcgtcaatga tccggcccgg ccaccgggat tcaaaagatt cgtaggtgcc gtcggtgttg
     9901 tataatacaa gaacgcagca aaaggaagcg gttctttttt ccggcggaac ctctttgagt
     9961 ttttccaaaa gcagattgat gttgtcgtcg tccgaggccc cttctccggc ataacgcgcc
    10021 gaataaattc ccggctcgcc gcccagcgcg tccacctgca gtcccgaatc gtcggccaga
    10081 acggcctgtg ctgtccgttc ggaaacggtt ctggcttttt tcagggcgtt ttccaggaag
    10141 gtttttccgt cttcgacgat ttccgggata tcgggataat ctttcaggga gacaagctcg
    10201 atgttgatgc aggaaagcat ttcccggatt tcccggacct tgccaagatt gccggaagca
    10261 aaaacgattt tcatccgatc aggtcaccca caagttcttt ctgccgttcg atgatctgcc
    10321 ggatgccgcg ctcggccacg tctgtcatct gtgtcatttg ttcccggctg aacgggattt
    10381 tctccgcagt gccctgcact tcgatgaaat gccccgaacc ggtcatgaca aaattcatat
    10441 ccacgtccgc gcgggaatct tcttcatatt ccagatccaa catcacctgc tgatcgatga
    10501 tcccgacact gacggccgac agataatcgc gcacgggaat gttctgcacc acgccgtctc
    10561 gtttcaattg atcaaacagg cccaccaagg cgacaaacgc gcccgtgacc gacgctgtcc
    10621 gggttcctcc gtccgcctga atgacatcgc aatccagata aatcgttttt tcgccgaagg
    10681 cgctcaggtc cgtcacggcg cgcagcgatc ggccgatcat acgctgaatt tcctgggtgc
    10741 gtccctggat ttttccccgg acggattccc ttgctgtgcg cgtctgcgtg gacatcggca
    10801 gcatggaata ttccgcggtc acccagcccc ggcccgtatt cctcagaaag ggcgccgttt
    10861 tttcgtcgat cgacgcggcg cagagcactt tcgtgttgcc gaattccacc atcacggaac
    10921 cggcggcggt attgagataa ttcttgatca gttttatcgg cctcagttca tcgaaccggc
    10981 ggcctgattt tctttcggtc acaactcacc tggaaaatgc tttgatgctg acggccagcg
    11041 ccttgcccat ttcagcctgc gtttttttgg acatcagatt tttctggtca acggcatttg
    11101 tggcaaagcc catttccacc gccacggccg gcaccagcac cccctcggtg acggacaaat
    11161 cggccatgcg caggcctctg gccttgcgcg gaaagagcgc gttgaaatta tccgaaatca
    11221 gcttggccat ttttaaagaa ttgttctgac actggctttg cggtttggtt ttgtcggtct
    11281 tccgcattga ggccggatcg tcggcgccgc aggacggata atagatttca tatccggagg
    11341 cttccttgcc gaatccgccg ttgacgtgaa gattcagcac catgtccggg ttgagggttt
    11401 tgatcatttt ttgtctggct tcccgatcca gggttttgtc cgtctcccgg gtcaggatga
    11461 tttggaggtt gggttcctgc gcaagttcct ttttgatctg cagcgccacc gccagggtaa
    11521 catctttttc ggaaacgtcg tcattgagtt tcagcccccg atcttttccg ccatgggccg
    11581 gatcgatcag aatgatgtat tttttctccg cggcaataag ctccgcagcg ccccagccaa
    11641 aacaggccag aacggcaaac gtgatgagca ctagaatttt ctttgtatga aacatcgtct
    11701 tctctctttt ttttagtcgg gcctgtttat aacagagtga ttttttttgt caatatgatt
    11761 taaaaatgat gcagccattc cacggccttg acaaacttca actgcctgat ggcggacatg
    11821 atttggttca actgcggcag attgctgatg tcgatgataa atttacaggt ggcgatcatg
    11881 ttgacggttt cgatttcggc atagctgata ttgacgtcga acgatgaaat gatggagctg
    11941 acttccgtca ggatgccttt aatatcgtca cagacgactt tgatgttcac gggataggtg
    12001 tgctcttcgc gggaactcca ctgcacgtcg acgatccgct cctgatccat tcgtttaata
    12061 taggggcaat tgctggtatg gacgatcacg ccgcggccgc gcgagatgta accggaaata
    12121 tcatcgccgg gaatcggatt gcagcatttg gcaaaattca ccatgatgtc gtcagggtcg
    12181 tcaatgccga tcagcgaaac gcccatggaa ggcgatgccg cttttttctt tttaattttt
    12241 tcccgggcgg gttctgccgc ttcgacttct tcgccggcga tgaaataatg ggcgacccgt
    12301 ttggcggaaa ttctccccag cccgacctgg aacaacagat cgtccagctg attcaccgaa
    12361 aacgcggaaa agattttttt aatgtcgtcc gatttgacaa tgccggaaaa tttcagatta
    12421 tgccgcttga attccttttc cagcagctcc cgtcccagaa ctatgctgtt tttcttttct
    12481 tccaggttaa tgtaattgcg gatcttggtg atggcccggg tggtcacgac atttttgagc
    12541 cagtccttgc tgggatggtg tcccgcctgc gtgatgattt ccacgcgatc gccgttttgc
    12601 agcttgtagc gcagcggcac gatgctgcgg ttgaccttgg cgccgataca ctgattgccg
    12661 acgtcggtgt gtatgctgta ggcgaaatcg atgggcgtgg cgcccttggg aaaggacttg
    12721 acgtcgccgt gcggcgtaaa tacgtagact tcctccggaa agagagccag tttcagatcg
    12781 gacatgaatt ctttcggatt tttgatgtcc tgctggatct ccagcacttc gcggagtttc
    12841 cggatctggt cttcttcgtt gtccttgtag gatccgcctt ccttgtagcg ccaatgtgcg
    12901 gcgattcctt tttcggccca ttcgtgcatg ttccgggttc tgatctgaat ttccacccgc
    12961 tcgccgtagg gcccgatgac cgtggtatgc agcgactggt aattgttggc cttgggcatg
    13021 gcgatgtaat ccttgaaccg tcccggaacc ggcttccaca gcgcgtgcac gatgctcagc
    13081 acttcataac aggatttttc cttgtccgca tcgagaatga tcctgaaggc gatcagatcg
    13141 taaacctcgt cgaaattgat gtgctgttca tgcatttttt tgtaaatgct gtagaggtgc
    13201 ttggcccgcc cctccacttt gcaggcaacg gaatatttct ccatttcctg ctgaatgata
    13261 tttttgactt cctcggtgta gcggttgcgg gcctcattcg atttgacgat cttggcggtc
    13321 aggtcgttat aggcgtccgg tgcgatgtac tggaaggcaa gatcttccag ctccatccgc
    13381 atggagttga tgcccagccg gttggccagc ggcgcgtaaa tatccatggt ttcgcgcgcg
    13441 atgtagatcc ttttttcctt ggggtgatac tgcagcgttc tcatgttgtg aatccggtcg
    13501 gcgaggcgca ccatcagaat gcggatatcc gacgacatgg cgagaatcat tttgcggaag
    13561 ttttccgatt gccgctcctc ctgcgacccg aaggaaatgc ggctcaactt ggtcagcccg
    13621 ccgaccagaa aggagacgtc ttttccgaat tcgtgctcca gttgatccgc cgtagtcagg
    13681 gtgtcttcaa tggtgtcatg cagcaggccg ctgatgatgg ttgcggcatc cattttcatg
    13741 tccgtcagga ttccggcgac ttccatcgga tgcgtcagat agggctcgcc ggacaaccgg
    13801 acctgcccct gatgaacgga tgccgaataa atataggcct tctcgatcat ttccagatct
    13861 tccgccggaa gataggtctg agctttttca ataatgtcgt tgatccgaag catgaactct
    13921 tcaaccctct cgtttcgcgg ctaggcgata tccggattca attcctgccg gatgatctcc
    13981 cggaccctgc cgacgatgtc ctgcagcggg cacaattcat tggcgcctgt ttttctgatc
    14041 ttcagttcaa cgttgccgtc ctgaagattt ttgtggccga cggttacccg cagcggaata
    14101 ccgatcagat cggcgtcctt gaatttgacg ccggcccgct catcccggtc gtcgtacaaa
    14161 acctccacgc cttccgtgat catggcatta tagagatatt cggcggtctc ccggatcgtg
    14221 atctcgttga tattcacggg ggtgatgatg acctggtagg gcgccagcgg catcggccag
    14281 atgatgccgt tatcgtcgtg attctgctcg ataccggcgg caacggttct gccgattccg
    14341 atgccgtaac agcccatgat catgattttt tcctggccgt ctttgcccag ataaaccgcc
    14401 ttcatggcct tgctgtattt ggtgccgagc ttaaacacat ggccgacttc aatgccgcgc
    14461 gccgttttga tgggctgtcc gcagcgcgga cagggatcgc cggcgttgac aattctcagg
    14521 tccgtgaacg cttcgacttt gaaatccctg ccgacattca cgtttttcag atggccatcc
    14581 tgccggttgc ctcccgtgac aaaattcacc atatcgatca ccgaataatc gacaagaatc
    14641 ggggctttga tattgacggg accggcgaaa ccgcgcggcg ccccggtgac ttcccggatc
    14701 atgtcgtcgg aggccagttc cagttcggcc gcggcgagat aatttttaac cttgacctcg
    14761 ttgacctcgt gatctccgcg gatcagcacg gcgcagggtt tgccgtcggc attgaagatc
    14821 agcgttttga cgatgttctg cggcgtgact ttcaagaagg cactgacttc ctcgatggtg
    14881 cggacatccg gcgtatccac gtcctccagg ggcagccagt cgtcttcaaa gatgattttt
    14941 ttctcgggac aggccacttc ggccttttcc agattcgccg cgtaggcgca tttttcgcaa
    15001 aacaccatcg cgtcttctcc ggagtcggcc atgaccatga actcgtggga atacttgccg
    15061 ccgatgcttc cggaatccgc ttccaccggc cggaattgca gaccgcagcg ccggaaaata
    15121 ttgttatacg cagcaaacat tttttcgtaa cttttctcgg cgccctcttc atcgacatca
    15181 aaactgtagg cgtctttcat gccgaactcg cggcagcgca tcacgccgaa ccgcggacgg
    15241 acttcgtcgc ggaatttggt ctggatctga tacaggttga gcggcagttg acggtaggtt
    15301 ttcacgtcgt tgcgcaccag gcccgtaatg acttcttcat gcgtcggccc cagacaatat
    15361 tcatgatcat gccggtcgcg gagtctcaga agttccttgc cgtaataagt ccaccgcccc
    15421 gattcctgcc agatttccgc cggttgaacc atgggcagat gcacttcctg cgcgcccgcc
    15481 cggttcatct cctcgcggat gatctgctcc agttttctga tcacccggta ccccagcgga
    15541 aggtaggaat agatgccgct ggtcagtttg cggatcaatc ccgcccggat catcaactgg
    15601 tgactgatga tttccgcatc ggaaggcact tcccggacgg tcggtaaaaa catttctgaa
    15661 taacgcatta atcctttttc ctttcttcaa tgatcgtatc gatttcttcg agtaaaatat
    15721 tcacaaaatc tttttctctg acttttttat aagcttttcc ttttttgaaa aggagcccct
    15781 gcccttttcc cccggccaga ccgatatcgg cgtccgccgc ttcgcccgga ccgttgacga
    15841 cgcagcccat cagggccact tttaaatagt ccttccggcc ggccagggct ttttcgatgc
    15901 ggcgcgtgag gctcggaaga tcgatttcgc accggccgca ggtcgggcag gaaacaatgt
    15961 ccacgccgac acggcggatg cccagcgcgc gtaaaatgcc gtacgccagc cccatctccg
    16021 aaaccggatc gccggtgacc gacacccgga gggtgtcgcc gataccctcg tacagcagcg
    16081 tcccgatgcc cagcgaagat ttgatcgcag ccccgatgag cgagccggcc tccgtgacgc
    16141 ccagatgcaa cgggtaatcc gtccgggcgg caatggcccg gtatgcttcg atcgtggcgg
    16201 gaacgtcgga agatttcagc gataatttga tcagatcgaa tccgcggtcc tcaaacagtt
    16261 ccaaattgcg cacggcgctt tccaccagag cctccggcgt ggcgccgccg tattttttca
    16321 gcaggtcttt ctgcagcgat ccggagttga tgccgatccg gataaccacc cggcgttccc
    16381 gggccagccg gatgatggcg cccattttgc ccggcgcaat attgccggga ttaatccgga
    16441 ttccgtctac gccctgcctg atcgcttcca gggccaggcg gtcatgaaaa tggatgtccg
    16501 caatcagcgg aatccggatt cttttcttga tggcggaaac ggaggctgcg gctttgtcgt
    16561 ccggcagcgc aatccgaacg atttcgcacc cggctttttc gagggcttta atctgtcgga
    16621 cggtcgcctc gacattcctc gtgtcggtgt tggtcatgga ctgaacaatc accggagcat
    16681 cgccgccgat tgccacgccc ccgagatgaa tctgttttgt ttttttcctt ctgataaacg
    16741 atgaatgttc cacagttgcc atttttccat tgattgaatc ggaagtcaat gacgacattt
    16801 gaattgatcc ggtaaaccgt ccgtcacgat gcggaaacga ggccgtcatc aaacgcttat
    16861 ttgcccagcc agcagtgcag gcacaactgg tccgccggaa gtccgatggc cgcgatcata
    16921 tcgtcaatgg ccagatattt caaggaggtc acgttcagat cccggcgaat ccaatcgatc
    16981 attttcgcgt gtttctcaga gtgatgatcc agatactctg acggatgttc gatttctttt
    17041 ccttccagcg cgcgaatggc cttgcggcag gccagttcac cggttgtccg cgtcgaggag
    17101 gtatagatac aggggaacat cagcggcgga caggccggac gcacatggat ttccttcgcg
    17161 ccattgtccc ataatttttt aatggtcaga ttcttcagtt gcgtgccccg cacgatggag
    17221 tcgtcgcaga tgatgatccg gttgccgttg atcacttccc gcacggcgca aagtttcatg
    17281 gtggccacca ggtctctgat ttcctgcgca ggcggcgtgt agcttcgtcc gtaaccgggc
    17341 gtgtatttga ccagcgcccg ccggtaggga atgcccgacg ccatcgcgta ccccagggcg
    17401 tgtccgacac cggaatcggg gacgccggag acaaagtccg cctggatatt gtcgcgtttg
    17461 gccagatagg cgccgcaccg ctcccgggcg ccttccacac tgatgccttc gtacgaggag
    17521 gatggatagc cggtatagat ccagaggaac gaacatattt ttttctcggt gcccgccggt
    17581 ttgaccggct taacgccgtc ggtggtcaga agaacaatct cgccggggcc gaggaactgc
    17641 gtcagttcat agccgagatt ttcaaacgag ctggcttcgg tggccaccac gtaggattga
    17701 tcctgcggat ttttgctttt gccgatggcc agcgggaatc gtccgaattt gtcgcgggcg
    17761 gcataaatgc cttccgaggt catcaccacg gcgcagatgg atccttcgat cacggcgcgc
    17821 atggaggcaa tgccctcgac gatgttgtcg ccgcgattga tgagacagga aacgacttcc
    17881 gcattattga tgtctccgcc ggccctttct ccgaagttgg cgccttcctt gagcagctca
    17941 ttgaccagtt gatttttgtt gttgatcagt cccaccgcgg ccaccgcata gacgccgaac
    18001 cgcgaacgga aaatcagcgg ctgaggcgta tcgtcatcaa tggccccgat gccgaagaat
    18061 ccgtccattt ttttatattc atcgataaaa cgggatttga actgaacctg ggacacgctg
    18121 tgcacggtct tgtaaaatcc ctggggcccg agcaccgcca tgcccgcgtg ttccgttccc
    18181 agatgcgaat ggtaatcggt cccgtaaaaa agcgttttga tgcaatttgc cgatgataca
    18241 actccaaaga tgccgcccat tgatttgtgc tcctaattag gtgagtttgg aaaccgcttc
    18301 cctgatccgc ctgatgcctt cctcgatgtt tttcagggag gtggcatagg ataaacggat
    18361 gtggctgtcg ctgccgaatg ccgctccggg cacgcccgcc acattggctt catccagcag
    18421 atagtcaata aaatcggccg aaccgtttat gaccttgccg ccgcaagaac gtccgtagat
    18481 gccggacacg ttggggaaaa cataaaaggc gccttccggc gagaaacagc tgatgccggg
    18541 aatttcgttg agggccgcga caatgtagtc cctgcgtttt ttaaattcag ccgccatatc
    18601 gcccaccatc gtctgatcgc aggacagcgc ttccaccgcc gctttctggg caatcgatgt
    18661 gggattggat gtgttctggc tttgaatttt gctgatcgcg gaggcgattt cttccgccgc
    18721 cgcggcgtaa ccgatgcgcc atcccgtcat ggcataggtt ttcgacacgc cgttgacgac
    18781 aaccaccctg tctttcaatt tcggctcgac gttggcgatg ttggcaaacg gaaatccggc
    18841 gtagaagatt ttttcgtaaa tatcgtcggt gatgatcaga atatccttgt cgaggagaac
    18901 ctgcgcaatc gccttgagtt cgtcgggaga atacgccgct cccgtggggt tggacggact
    18961 gttgagaatc agcgcccgtg tgcgggcggt gatggacgcc gccagctgtt ccggcttcat
    19021 tttaaaggca tccttctctt cggttttgac gatgaccggc gtgccgtccg cgagggtgac
    19081 aatgtccgga taggacaccc agtacggcgc gggaatgatg acctcgtcgc cggcgtcaaa
    19141 ataaatctgc gcgagattgt ataaggtgtg tttggctccg caggaaacga cgatctggga
    19201 gcgtttgtat tccagacggt tgtcgcgttt gagcttgttg atgatggcgt ccttgagttc
    19261 atccgttccg cccaccggcg tgtatttggt gaatccgctc tgaatcgcct tgatcgccgc
    19321 atctttgata ttatcggggg tatcgaaatc cggttcaccg gcgccgaaac cgatgacgtc
    19381 tcttccctga gcccgtaaag cattcacttt ggccgtgatc gccaaggttt ccgacggact
    19441 gattctgcct actctcgctg ataatcgcac aacttacccc ctggtattgt atttttccgt
    19501 taatcgctga tagacattgt ccggaaccag tcccttgacg gacccgccca gactcgccac
    19561 ttccttgatc agtttggaac tggtaaaaaa ccatttaaag ccgctcatca gaaagacggt
    19621 ttcaatgtgt ttggtctggc gtcggttcat caacgccatc tgaaattcat attcgaaatc
    19681 ggacaacgcc cgcatgccgc gcagaatgat gttggccccc gacattttga gataatcgac
    19741 cagcaaaccg gagtagcaat ccacgcggat gcccggtgtc tccccgaata tttcgcggat
    19801 catttccatt cgttcttcaa tgttgaacag atagacttta ttgggattgt aagcgatcaa
    19861 aacgatgatc tcatcgaaca tccgtttccc tcttttgatg atatccacat gtccgttggt
    19921 gatgggatcg aaggaccccg ggtatatcgc tattcttttt gtaaaatctt cctgcattat
    19981 tctctctcca ttttaaaaaa agttaaggcg ttttcgccgt attttctctg atcggtccgg
    20041 tgaaaaattc ctaagaccgt ttccggtatc ggttctttca cggaatgctg catcaccagg
    20101 ataccgcctt tcgccaggac cgggtatttg cccaacagct ccagggtcac ggcgaccaga
    20161 ccgcggtgat acggcggatc ggcaaaaatg atgtcgaacg aacactgttt tctaaaaaga
    20221 tcgcgaaggg caaatgccac atcggttgcc atgatggtca accgtccgtc ctgcaagccg
    20281 ttcggaatat tctttctgat cacgccggca agtttttgat tgttctccac cagaacgatc
    20341 tggcgcgctt cgcggctggc ggcctccagg ccgacactgc ccgatccggc aaaaagatca
    20401 agaaatgttt tttcgttcaa agaaccggtc aaattgaaaa gcgcttcccg taaaaaatcg
    20461 gtggtcggcc tttcctttga tcccgaaggg aaaagcaaca tctttcccct ggcttttcct
    20521 cctgtaattc tcatatccgg tttaaaaata gttttttaaa gatgatgccc ttacggtttt
    20581 ccattcgctg cattgttttt gttttccggg acggccttcg gtttgccgaa gccaaacttg
    20641 atcagccacc agacgggccc cgacagggcg tatcccagga tgatgacgaa aaacattatc
    20701 tccggttccg cgacaatgat gatgagcaga acggttgacc agaagaaaat cgtgaagggt
    20761 ttgcggacca gcagagacat gtctttaaaa ctgtaatact tgacgctgct gaccatcagt
    20821 aaagagatca tgatgacaaa gaccatgatg aagatattgt ggaattttcc ttccgctccg
    20881 acatagtcga agaagatcac ggtggtcgca atgaccgacg ccgcggcagg aatgggcagg
    20941 ccgttgaaga ccttgctttc gatgatgcct atttgaatgt tgtaccgcgc gagtctcaaa
    21001 gcgccgcaca gcacaaacag aaatgcgacc acccagcccc acttgccgta aacggaaagc
    21061 gaccagctgt aggccagaag ggccggcgct atgccgaagg agatgacatc cgccagggaa
    21121 tcgtattcgg cgccgaattt gctggtggtg ttggtcagcc gggcgacacg gccgtcgagc
    21181 ccgtcgaaga tgacggagac cagaatagcg attgccgccg tgacaaaatg ttccttgaac
    21241 gatgcgatgg cggagtagaa tccgcaaaaa aggctggccg aggtgaataa attgggcaac
    21301 aggtagatgc cctttttcat gcggattttc cgcgctgcat tttccacgtt catgataaat
    21361 atcctaaaac cgtttggccc gccttgactt tatctttcaa tttgaccgat atggcggaat
    21421 ccacgggaag atagacctcc acccgcgatc cgaaacaaat gatgccgatg cgctccccct
    21481 tgcggatagg catccccggc gtcacccagc agacgatacg ccgcgcgatc agaccggcaa
    21541 tctggaccgc ccagaccgcg cggttgtccg gcgtgcggat cagaatttca ttgcgctcgt
    21601 tgtcttccga cgctttatcc agattcgcgg aaaagaattt cccgggatga taatgaacgg
    21661 cttcaatttt gccgtcatag ggagcccggt tgacgtgcgc attgaaaaca ttcataaaaa
    21721 tgctgatttt tttgaatttc cccgagaggg tgccgttgac gtccacctct tcgattttga
    21781 tgattttacc gtcggcggga ctgattaaaa ccttttcgtc ttccggaaaa gaccgctccg
    21841 gattgcggaa aaaacagagg atgaaaaccg tcacaagagc gaaaaagagg atcaaccacc
    21901 agttaccgcc cagaaaggcg acaaaaactg tgacaatcag ggaaaaaact acaaaaggat
    21961 atccttcagc ggcgataata ctgtcatgct tcataaatac ttatcttttc cgaataaaaa
    22021 caagctttta atatcacatc cacataaccg gcaatcacga tctgcatttc ccgtcagacg
    22081 tcttgtcgtt aaacgagttt ttctgatata tcaattgacc gtgcttgtca ataaatcttt
    22141 aatttttata caaaaacatg ttggatttcc tcccatgttt gttattgcat cgctccggtg
    22201 tctcctgtat attaatcgat aatgattatt attttttaag gttttttgca tgacgcgccc
    22261 gtttaaatgg ccaatggccg ccggaattct ggtcacaatt gcctttgccg ttttgctttt
    22321 gatccgtctt gatttttttc agacgcaaac gcctccggtc gcggggataa aaatggtccc
    22381 gccgcaacaa ccgcaggata tctggatgac gattttccag aacgaccgga aaatcggatt
    22441 tgtccaccgg atgctgacga aaaatgaaaa cggatttgcc ttcaacgaaa atgtttttat
    22501 ggaaatcagc accatgggga tgccgcaggc gctgaatctc gccactgaag gacagctaaa
    22561 cgcggacatg accctttcct ccttcacttt caaattaaat tccagtctct ttcagtttca
    22621 tatccgcggc cgtgtggccg acaacaaact gatcctttac accggtgccc caaaccgtca
    22681 gcaaaaaaat gaaatgccgc ttcaggccct gccgcatatc agcggcaaca tctacgacgc
    22741 ggcctttcac gccggcatgg ataagggcac aacccgcgga ttcagcattt tcgatccgtc
    22801 aagcatgtcg gtccggacga tcaacgtcac gcgcaacgcc gatgaaatta ttccgatcat
    22861 gggcaatcgc ctgcttctga aaaaatactg cgccgatttt atgggcgcca aacactgcgc
    22921 atggctggac aaagacggac aggtgttaaa agaaaccggc atgctgggtc tttccatgga
    22981 aaaaacaagc cgggaaaatg cgctggcgga aatcgacggc agcgcaggcg tggattttac
    23041 gcaaattgct tccatcccct ccaatgtgga catcgccgat ccggaaaaac tccgcaaact
    23101 gcaaattagg atcagcggga ttaccccgcc gctgcagcgt cagctccatc tcgacggcga
    23161 tagacagcat tatcacgatg gcgttctgac catcacgcgt gaagatcccg cggataaagg
    23221 tcctgcaggc ggttggcctg cggatatcgc cgtatttctg aaacccgccg cattgattca
    23281 atccgatgat ccgcaaatca aggcgctcat cgggaaaatc gtccgtccgg aggattccgc
    23341 ttttgtcaaa gcacggaaaa tcatcaactg ggtttttctg aatatcgaaa aaaagccggc
    23401 tctgtcggtg ccgaatgccc tggaggttct gaaaaaccga tccggcgact gtaacgaaca
    23461 tgccgttctg acggtggccc tgttgcgggc agccggcatt ccggcccggt ctgaagccgg
    23521 tctggtgtat ctgaggggac gattttatta tcacgcctgg tgcgtgcttt acatcaaccg
    23581 atggataacc gccgacgcgg tgttcaacca gattcccgcg gatgtcacgc atatcagact
    23641 gctgcgcggc gaaaccgatc agcagatccg cttaatcggg gtgatcggca aaacaaacct
    23701 ggaggtgctg tcacaaacga gatgatcaca ttatccgaac tgaccaagga ttacggaacg
    23761 accgtcgccg tggacaaact cagcctgacg gtgaacgggg gggaaattta cggctttatc
    23821 ggtcccaacg gcgccggcaa aaccacaacc atccgcatga tgggcggcat tcttgcgccg
    23881 acttccggca gtattgtgat cggcggtgtg gacatggccc gaaatcccgt cgaaggaaaa
    23941 cggatgatcg gttttgttcc cgatagacct tttctgtacg aaaaactgac cggcatggaa
    24001 tttctggagt tctccgctaa tctgtataac gtccgccgcg gtcttttccc tgaaaaagcc
    24061 aaacgcctcc tgcagcaatt cgccttgtgg acctgggcgg atgaactgat cgaagcctac
    24121 tcccacggca tgaagcagcg gctgatcatc gccgccgcgc ttttacacga tccgcagatt
    24181 ctcatcatcg atgaaccgat ggtcggactg gatccggaag ccgtgcacat ggtgaaggat
    24241 attctgaaag acctcgcggc gagtcaaacc accattttcg tttccacaca tacgttgagt
    24301 attgccgaag atctctgcca ccgtatcggg ctgattcaca agggcacgct gctggccgag
    24361 ggcagcctca aggaattaaa gcagatcgcc cgactcggcg aagcgaggct tgaggaagtc
    24421 tttctgacga tcattaaaga gaacacgttt ctatgaagac ccttttatcc ctgatcggac
    24481 cgcgcgtgcg gtcttcgctc aacagtctga cccgcggcaa caggaaaaat gtctggtcgc
    24541 gccttgtcat gttcggcgga ctcggtcttt tgttctgggc gggcatcttc atgattttct
    24601 accgcgtcct gctgtatttc cacagcgtgc aggatttcgg agacattctg gccatgaagt
    24661 ttgtctccat gattattttg accttcttca tgcttttgtt gttcagcaac atcatcaatt
    24721 gcctctcgca cctttacctg agtcaggatt tgccgcttct gcattctctg ccggtatccg
    24781 caaacgacat ctttttttcg cgctggatca tcagcgcttt cgacagttcc tggatgattc
    24841 tggccttcag tcttccggtt ttcctgtcgt acgggatcat ttttcacacc ggcatcttct
    24901 attacgccat ttttacgacc gtcatgattt tcatgtgtct gatcgccgcc gccctgagcg
    24961 ggatcctggt gttgttcgga tcgaaagttc tgcctgccgg acgcatcaga accattctga
    25021 ttctgctggg agcgctgatg atgattgttc tgattttcgt tctgcgcttc acacggcccg
    25081 aacagctggt caatcccgac agttttgcat cggtggtcct ctatctgaat gcgctgaaga
    25141 cgcccagttc accggtcctg ccgacaacgt ggatcaccga cgccatcctc gccgctttga
    25201 aaaacgaccg cgcaacggcg cttctgaatg cgggcctggt ggtcaccttt gcctgtatgc
    25261 tcgtttttat cagcagtatg cttgcccgga acatgtattt tccgggattt tcaaaatcgc
    25321 agatgacacc ccgtcgtctg tttgcgcctt tgcggtacaa aggattctcc tgggaaacgc
    25381 ttctgaactt cctgccgcgt gaatccaaag cgttcgccgt caaggaaatc cggacatttt
    25441 tcagggattc ctcccagtgg ccgcaacttt tcctgatgac ggcgctgatt gtgatttaca
    25501 tctacaattt ttccgtactg ccgctggaga gttatccgat taaaaccatt tatctgcaga
    25561 acgtgttttc ttttttgaac atcgggctgg ccgctgctgt tctcgccgcc gttgcggccc
    25621 ggtttgtctt tccggccgtc agcatggaaa gcgaagcgtt ctggattgtg caatccgcgc
    25681 cggtaagcat cggcaatttt ctctggatca aattttttct ctattatatc cccctgatga
    25741 tgatggccga aggcctggtg gtcgcgtcca acattctcct caacgtttcg gatttcatga
    25801 tgctgttgtc cgtcctgacc gttttttttc tggttcccgc cattgtcgga ctggccacgg
    25861 gactcggagc cgtttacgcc gatttcaaat cggaaaatcc ggccaacatc gtaacgggtt
    25921 ttgggggact gctttttatg attttaagct tcggtctgat tgcgcttgtg atcatcctgg
    25981 aggccggacc ggtctatacc attttcatgg cggacgttct cggaaaaaat ctcacggccc
    26041 tgaaaatcct gtgggcggtg ctttctttcg cgctggcgat tttcctgtgt ctgctggcga
    26101 cattttatcc gatacatctg ggccggaaac gcttaaccgt aaaataaata ttttttcata
    26161 ggtgattttt ttgaaactca ggctacacgc atgccgaaac gacatggatg aacaaaattg
    26221 tcttgatttt acttaataaa taagctacat tgcactaaat taaatttaaa ttctacagtt
    26281 tccagctaag attgggaatg tgaagctttt tttgattgta atagaccgca aatgttttcc
    26341 cggctgagaa ctgattagag gagagaaact gattttttat tgatggattg tattgtaccg
    26401 gatcaatctg ttttgcagac ggatttccgt gaataattta tagcgatccc agaccacagg
    26461 agtcatatgg aaaagatttg cattgtaaag aggcgtcgag aatacacaca accggaagta
    26521 aaagtaacgg ttacgcaacc ggccatcagt gaaacggccg cccccgaagt tgccgtcagc
    26581 cgcgtcgacg ttaaaaacaa cgcgggatta tcggagaata cagcccggac aacaaaagaa
    26641 agcatgacgc ctgaattgac aatccgcagt gtggggatgg agcatgaaat caagataccc
    26701 gaacgaaaaa ttgtcgaaga attcgtggac aatgcgtcgt ccgtcatcgc catcaccatg
    26761 accaaggagc aatccgatct tttactgcaa tccgaataca tcaaagaact gctcagcggc
    26821 aagaaaagcg atcccgcttt ggatatcaag gtaacgcctg aagggagact gtccctgaat
    26881 taccgattca gcgattcgtt gcttttgcga atgatcgcgc cgaaccaggt gtgccagatg
    26941 cttcaggtca gccgcagctt tttgcagaag ctggttcatg aaaacaagat taaaagttac
    27001 aaactgggaa gaatgagaag gttcctgctg gaagatatcc tggagtattt gagcagcgag
    27061 gttgatttca tgcagccaag cgatgacaaa taaatgggtg cgtgttttat tggctgaaaa
    27121 taattttttt gcttttacaa ggcgaaaccg aggagaagaa agatgtattg tgattactgg
    27181 aatttaacta agccaccttt cgacaatgtt cccgatgcct ccatgtatgt ggattgtcat
    27241 gaatccatgg aaaatgtcat ctccgaaact ttatttgcca tcaaagaagg caacgagtgc
    27301 cttacggtta ttgtgggcga agtcggcctg ggaaaaacgc tatctcttcg cgtgatcata
    27361 gactcgctgg aacctgaaaa atataaagtg gcgttaatta ccaatccggc gctctcgttt
    27421 gttcagttat tgcgggaaat catcggtcag attaccggaa agcagtgcga agtcaaaaac
    27481 aaggtcgatt tgcttgaaat attcaaccgt cttctgtttg aaacggcaga tcagggcagg
    27541 aaaatcgtca ttgtcatcga tgaagccaac gttctgtctc cttccaatct tgacgatctg
    27601 cggcttctga ccaatatgca ggatgatgaa cgaaatcttt tcactctgct gctcgtcggc
    27661 cagatggaat tcgccaaaac gctggaacat cccaagcggg caaatctctt tcaacgtatc
    27721 ggcacatacg gtcatctgga aaagctaccc tctccggaag ccgtcagaac gtatatcgaa
    27781 tcccggctca gaatcgccgg cacggacaga aaaattttcg acgacgatgc catcccggtg
    27841 atctgggaat tttccgaaca tggcgtgccg cgtctgatca acaaaatctg caagttgtgc
    27901 ataaaagcgg gtgaaacgaa tgaatttcag accatatccg gtgatgtggc ccagcaaatc
    27961 gccgatcgtt tccagaaatt aagcaagaca tccgctcaaa aacgcaaagc gcctgccgcg
    28021 gaagcacaac ctgccgcaga agctccccgc aagcaggagc cggaaaggat accgaaaccg
    28081 tcgcgacctg ctttccccga aaaagagaca ccggctgttt ccgcgccggt ccggccggcg
    28141 ccgccgcgca tggaagagcc ggcgccgatg agaccgagaa tggctcctcc ggaacaaccg
    28201 ccggcaagac ccacagcgcc gcccgtcata gatgaagcgc ctccggtcag gcccagaccc
    28261 gttatgcccg aacagccgcc gatgcggcgc ccccttcacc cgggcatgga agacctgatg
    28321 ccgccaagat caagaatggc tccacctgaa cagccgcctg tcagcccggc tctgccgagc
    28381 atggaagagc cggcgccgat gagaccgaga atggctgccc ctgaagagat gccgctcaga
    28441 ccgacagcgc cgcctgtcat ggatgaagcg cctccggcca ggcccagacc ggtgatgccc
    28501 gagcagccgc ctgtcagtcc ggctccgccg cgcatggaag agcctgcgcc gatgagaccg
    28561 agaatggccg cacccgaaga gatgccgctc agaccgacag cgccgcctgt catggatgaa
    28621 gcgcctccgg ccaggcccag accggtgatg cccgagcagc cgcctgtcag tccggctccg
    28681 ccgcgcatgg aagagcctgc gccgatgaga ccgagaatgg ccgcacccga agagatgccg
    28741 gcatataccg cacctgtgcg ccaggaagag ccgataccgg tccgtcctca ggcggttcca
    28801 gtaccgccga aaccttcggc aagcgttgtc ccgcctccgg tgagcgctcc gggtaatgtt
    28861 gctcctccgc aaggcgtccg cgctgcggac acggctcaac agtcttatga agagcagccg
    28921 atgccgatgg aggcagttga agaggaagat tttgaagagg tcaccatcgg tcagcagtcc
    28981 attaaactgg cgattccgtc gcatgtcatc cggcaggcgc agtcatccaa caaggaaaat
    29041 aagaacaaat tagcgggcta ctggtcggcg caaattttaa agggaaatcc gcagttaacg
    29101 cattcttctt ttgctgatcc ggtatcttta tggtttgaag tcaagaatgt cattttgaaa
    29161 aaattcaaca gccctgcaca catgcagtga tttgatttca aatggatgaa aattcgaaaa
    29221 tctaaatata tttatgtctt aatcgggcgt gtcttgatca tcacgaaatg aatatcgcaa
    29281 attccaaaaa tatcaataaa gacaactatt cgccttcaga ttatgaaaac tggggtttca
    29341 tctacctgga aaacaaagaa tttgatcaag ccttgaaatg ctttcaaaag accatcgaac
    29401 ttgaaccgca caacccgaag aattatatcc atctgggcta tgtttatgaa aagatggata
    29461 tgctggattc tgcaaaaaaa tcctacgaaa aagccctgga ggtcaaaagc gattatatcg
    29521 aagctatttt aaaaatcggt catattctgg aaatgcaggg cgattatcac aacgccgtgg
    29581 aactgtatac ccgagcgatt gacatcgcac ctcaggcgat cgaaccgcgc ttttgcctgg
    29641 caacccttta cgacagacac gacatgtatg acgaggcggc ggaagaatat gaaaacatca
    29701 tcgagatcaa ccccaagcat accaaagcca tccacaatct tggacgaatc tattttcagg
    29761 acggggatta cgccagagcg ctgaagagtt tccagaaagt tttaaatatc gaacccgaaa
    29821 ataccgatgc ctggaatgat ctgggatcgg tttatgaaat caccaacaat ctgatccagg
    29881 ccatttcgac ttaccgcaag gcgctgaagg tgaatccgct tcacgaggat accaactata
    29941 atctggccaa tgcctatttt tccctgtttt tatccaatcc gaatatcgtc aaaatcgatg
    30001 atattgtcga acggttgcag ctcgttctct cgcagaatcc caacaataga aaagtctggg
    30061 aactgctcag caagatcaaa gcgtcatgat gcctgcggga agtcatttcc cgaaaaagga
    30121 ttttttctga tcggcatccg ccgagcgcaa atagaacggc acaaagtttt cggcattcag
    30181 ccgttccccg cgttcatatt ttgctttgcc taaaacagcc acggccgaag cgcggcaatg
    30241 ctgttttgcc gttgaaagaa ttccgttttg ccgttcgggc ataaatgcgg ccccgtattt
    30301 gataaccccg ctgccgacgt aaacggcatc ggcgtcataa acgatgtccg acggattggc
    30361 aacctgttcg ccgccttgtt gttcgatcat gccgccggcc ccgtattgga agcaggccgt
    30421 gtagacctgg ccgcgccccg catccatcag cgcatatatt tttttcgagg ttttctccgc
    30481 gttcagcgca atggcggcca gggaggaaac accgaccgcc ggcttgccgg tggccagcat
    30541 gaatcccttg agcgtgccgg cgccgatgcg cagaccggtg aaggatccgg gaccgagggt
    30601 gcaggcaaaa acatcgaatt cgggaagggg gattttggtc cgcagacagg cctggtcaat
    30661 ggcgggcaaa agcgtttccg agtgattcaa gccggtatga atgatcacgt catagagaat
    30721 atcgtcatct tgcaggacgg ccacggaagc cgtttgggat gaggtgtcaa acgccaggat
    30781 ccgcatatca tttaaaaaat tttacaacat cgttgacgaa ttttatgttt agccgttcga
    30841 tatcgatgaa aataacgaaa agcatcagca tcagcaaaac gacaaacccg atttgctggg
    30901 acatctctct caccttggcg ctgacctccc gtctggtaac gacctcgatc aggtaaaaca
    30961 ggatatggcc gccgtccaat accgggatgg ggaaaagatt gatcaccccc agattaatcg
    31021 acaaaatcgc catgaagaga acaaacggaa tgatgccttc cctgacctga gcgcctgcaa
    31081 cctgcgctat gaagatggga ccgcccagcg ttttcggcga aatcacgcct tcaaatattt
    31141 taattacgga gataaccgtt aatttgctga tttcccatgt tttggccacc gacgaaacga
    31201 cggcatcaac gggacttttc ctctcgataa cggtttgtcc cgcgggagac accccgataa
    31261 gatacgcaga aacgtcttcc ccgaaaagat ttttcgattt ggacaatttc ggtgtgattt
    31321 gcaaccgcac tgtttctttt ccccgctgca cgacggcttc gatctgtctg tctttagccc
    31381 gggcaacgac atcccggatc tcttcccata aggcaatctt tttcccgtcc agagaaatga
    31441 ttttatcgcc gttaagcatg cccgcctgat acgctgcgga atccttctgg acctcgccga
    31501 tatccgacgt cagcgtcggc aaaccgtaca tcatcacggc aatgaaaatg actaccgcca
    31561 gtaaaaaatt gaacgcggga cctgctgcga cgatcatgat tcgcttccag acattttgcc
    31621 tggcgaatga tcgtttttca tcttccggcg ataattcttc ggtgccggat tcacccatca
    31681 gcttaacata gcctcccaga ggaatccatg acagcacata ctccgtttca ccgattgtcc
    31741 tgccgataat tttcggtccg aagcccaggg agaatttttg aactccgacg cccgacatcc
    31801 gggcgaccag aaaatgtccc agctcatgga caaaaatcag aatccccagc aatatgataa
    31861 aagaaattaa agtggtcacg ttgttatcct tcctattatt ttttcggttt gttgtcttgc
    31921 gtcggcatcc gcctgcaata tgtcatccat tgaaggatcg gggataacat catgcgcatc
    31981 gagaaccttt tcgatgattt tgggcagatc ggcaaaagag agtctttcct gcatcaatgc
    32041 cgccacagcc acttcatcag cggcattcaa aaccgccgga gccgtgccgc cgctgacccc
    32101 tgcttcacgg gcgatcttca aacaggggaa ttttttcaga tccggtttgt aaaaatccag
    32161 ccggcctgcc tgcacgagat cgagtgtttt caaatcattg gcaattcttt cgggatatgt
    32221 caaggcataa gcgatgggaa ttttcatatc cggtacgccc agctgcgcca gacaggcccc
    32281 gtcgataaac tccaccatgg aatgcacgag gctttgcgga tgcaccagaa catcgatctg
    32341 gtcaaaagac gtattgaaaa gccaccgggc ttcaatgatc tccagcccct tgttcatcat
    32401 ggaggcggaa tcaatggtta ttttttttcc cattttccag cgcgggtggc gcagggcctg
    32461 acgcaacgtg accttcttta actgacgggc cgcaacgttt aaaaacggtc ctcccgaagc
    32521 cgtcaggata atccgcttta aatcctcttt tctgcttcct tccagacact ggaagatggc
    32581 gctgtgttcg ctgtccacgg gcagaatccg gacgttcttt ttcgccgctg cgcgcgtgac
    32641 gatccggccg gccatcacca tggtttcctt gttggccagt gcgatgtctt ttccggcctc
    32701 gatggcggcc agtgtgggta atagtcctgc cgctccggaa atggctgaaa tgacgatgtc
    32761 cgcggacgaa caggccgcgg tttcctgcag tccttccgta tcataactga tccgaatccg
    32821 ggttccgggt ctcagggccc ggcgcagttt gacggccgtt tctttgtttt ttacggcggc
    32881 catctccggt ttgaatcttt cgatctgccg cttcagcaat tgaatatttt caccggcagc
    32941 cagagccgca atccggaaac gcttcggatt cttctcaatg atatccagcg cactgaggcc
    33001 gatggagccg gtggagccca ataatgtaat ttttttcatt taacctatca caaatatccg
    33061 gtaataataa acaaagggag cgatgaacat cagacaatcc agacgatcga gaatgccccc
    33121 atggccggga aggatcgaac cggcatcctt gagaccgtaa cctctcttaa tggccgattc
    33181 acaaatatcc cccagttgtc cgatcattcc gccgacaaac gccatgaccg ccacatgcac
    33241 aaccgctaca tccggcagca gataatggct gtagatcaga caggcaacgg tgctgcccac
    33301 caccagaccg atcagtccct ccacggtttt gcccggactg atcagcggaa ccagcttgtg
    33361 ttttccgaaa tacttgcccg tatacagcgc cacggtgtca ccggcaaagg ccagcaccag
    33421 aacaaacatg atccaccaga caccctggtc cagcattctg atggcaatga aatgggacat
    33481 caggaaaggg atatacatga tgccgaacac caccttggcg acggaaagaa aatcaaaaga
    33541 cgattcccgg atggacccga taaataaaac aaacaccagc atgaccgcca gcgcaaggag
    33601 cgccgtcaga tactgatggt tgccggacaa aacgcataac ggaattaaaa cggcaaaagc
    33661 aaggctttcg attttttcca tgacaaagcc ttgtccgaac actatgtgat gatattccca
    33721 gacgcctccg caaatgaaca gaatcacgac ggcggcaaac atcgcttccg acccgaagaa
    33781 aatcaccagc aggagcaacg gaacaagcac aatgcccgtc agccatctct tgatcaggga
    33841 actgggtgtc ttcgccatga tttttcacct tctgccttta ttgttttgcg ggatgctatt
    33901 cctgcagttg ctcgctggtc agaccgaaac gcctttccct cttctgataa ctggcaatgg
    33961 ccttcagaag atcctcccgg gtaaaatcgg gccataatac atccgtaaaa tacagctccg
    34021 tataggccat ctgccagagc aggaaattgc tgatccggta ttctccgctg gtgcggatca
    34081 gcaaatcggg atcgggcatc tgagccgtgt acaggtaacg gctgaatgtt tctttatcca
    34141 ggcgatcgat atcgagactg ccggtttgag cgtcccgcac cacagtcttc accgccgcca
    34201 ggatttcatc cttcgccccg taacttaagg ccagattcaa aaccatcttg tcattttgcg
    34261 ccgtacgatc gatcgtctcg atgagttttt cccggaccgt ctcgtttaac cgatccgttt
    34321 ctccgatggt ggtcagacgg atttcctgtt tctgcagttc gtccagttcg gcattcaaat
    34381 attcttcaag cagatacatc agcgccttga cttccttgtc cggccgctcc cagttttcga
    34441 tggaaaacgc aaaaagggtc agatatttga cgccgatttc ccgacaggtc cgcaccgtcg
    34501 ttcttacggc ctccgctccc tttttatgcc cccggattct ccccatggca tgctgcttgg
    34561 cccaccggcc gttaccgtcc atgatgatgg cgatatgacg gggcaattta ttgacgtcga
    34621 ttttaaccaa acacgtatgc tcctgaatga tggtctatcc taatcgaccg ctgatttttt
    34681 ttcaacagtc aattcccgga taattttttt gaatggcatc ctgcctggac agaaataatc
    34741 aaaacaccgt attaaaaaca aatggggagt cggaaactcc ccatcgtcgt tttcgtatca
    34801 ggcgtttgtt aaatttccat gatctctttt tctttggccg ccagaatttt atccgtttta
    34861 tcgatatact gatcgatcag tttctgtact tcgtcctgcg cggtaaaaag ttcgtcttcc
    34921 gatattttgt tgtctttctt taatttcttt aattcttcgt tggcgtctct gcgcgcattg
    34981 cgcagtttca ctttcccctc ttcagccatt tttttgacgt ttttggctat ctctttgcgg
    35041 cgttcttccg tgagctgcgg aatgctcagt cgtacgacct tgccgtcatt ggccggggtc
    35101 aggcccagat ccgatttctg aatggctttc tcgatggcgc cgataatgga aacatcccag
    35161 ggaacaatta aaatcagacg gctttcagga acggaaacat tcgccacctg ggcaatcgga
    35221 gtcggagcgc cgtaataatc gactttgata ccatcgagca gcgaaaccga tgctctgccg
    35281 gttctgattc tgctgaacga tttgcttaat ccggcaatcg ttttttccat gtcttcctta
    35341 aatttctgaa aaacggtttc cttcatgatt attctcctac gtaagttccg attttttgtc
    35401 cgcaaaccgc tttgcaaata ttgcccttct tctgcaaatt aaaaacaatt atgggcaata
    35461 aattatcacg gcaaagcgat attgccgttg aatccattac tttaaggttt tttgttaaga
    35521 catcaatata acttatttta ttaaacttga ccgctttttt gtccctgacc gggtccgcgt
    35581 tataaacacc atccaccttg gtggctttca tgatgacgtc agcccggatc tccaccgctc
    35641 gcagcgccgc cgcggtgtct gtggaaaaat agggattccc cgtgccgccg gcaaatatga
    35701 cgatgcgtcc tttttccaga tggcggatcg cccgtctctg aataaacggt tcggcgattt
    35761 catgcatttc gatggatgac tgcacgcgtg tgggaagacc gcgttcctcc agcgcgctct
    35821 gcagggccag gctgttgatg accgttgcca gcatgcccat gtaatcggct gtcgtgcgat
    35881 ccatacctct ggcgctggct tcaatcccgc ggaaaatatt tccgcccccg atgacaatgc
    35941 cgagttgaac acccattctg gaaagcgact ttatttcatc ggcaatgtaa ttggccacat
    36001 ccggatcgac accggtggac tgtttcccca tcagggcttc gccgctgagt ttcaggagca
    36061 ctcttttata tgccgccttc tgctttgtgc tcattattgt ttgatttcct cacccagttg
    36121 aaaacgggca aaacgacgga taaccatgtt ttctcccgtt ttggcaatca tattgttgat
    36181 cagtgtcgca acggtgatgt cggtatcttt gatgaatttc tgttgggtca ggcagacatc
    36241 ctcgtagtat tttttgagtt ttccctcgat gatcttatcc cagattttct ctggtttctt
    36301 ttcttcgcgc aactgactgc ggtagatttc cttttccctc tccagggcgg actccggaat
    36361 ttcctccgga cgaacgtaaa gcggattgga cgccgcaatg tgcatggcga tgtcccgggc
    36421 catggtctga aaatcttccg ttttcgccac gaaatccgtt tcgcagttga tttccaccat
    36481 gacaccgatt ttgccgccca tgtggatata ggaggcaacg gttccatcct tggccgcttt
    36541 cgacgaccgc ttcgtcgcgg cggaaagccc cttctttctg agaaaatcga tggctttctc
    36601 aaaatccccg tctgattcag ccagcgcctg cttgcagtcc atcatgccgg cgccggtttt
    36661 cgttcttaat tctttcacca ttgttgatgt tatttccatg tttacttttc tcccttacat
    36721 gctattctaa attccagaca gattccgatt attcggcgta cgtttccgat ttatcgatac
    36781 tatcatcaat ttccagactt tccggaatat ccgcttccac cggtttggtc tccacggcgg
    36841 catccgcttc cttattcgat tcagcagcca gtttttcttc aaaacgcttt ttcccctcca
    36901 aaactgcatc ggcaaattta gaggtaaaca gtttgatggc cctgatggca tcgtcatttc
    36961 ccggaatcac gtaatcaata tcatcgggat tgcaatttgt atcgacgata gcgacgattg
    37021 gaattctcag tttcttagct tcattgacgg caatatgctc cttgccggga tcaacgataa
    37081 aaacggccgc cggcagagac ttgatggtcc ggatcccgcc caagacgttt tccatttttt
    37141 ccttttcctt ctgcagtttc gtaatttctt ttttgggaaa agctttaatg gagtcgtcat
    37201 tgaacatgtc cgtcagcgag ttcagacgat cgatgctctt tttgatggta acgaaattgg
    37261 tcaacatgcc gcccagccag cgctgattga caaaaggcat accgcaacgc gttgcttctt
    37321 ccttaatggc ttcctgtgac tgtttttttg tcccgacaaa gagaatttcc ttcccttcgc
    37381 tgacggtgtc gatgacaaag ttatacgccg actggaaaag ccccacggtc tgctgcaaat
    37441 cgatgatata aatgttgttt ctggctccga agatgtacgg tttcatcttg ggattccatt
    37501 tgtttgtctg atgcccgaaa tgaacaccag cctccagtaa aagtttcatt gaaatcgctg
    37561 acatatctct ctcctttttt gtttttttcc tccatcccct tcaccttggc cggccaactt
    37621 gaccttcaag caacaaaccg gcaatccggg gatgtgtgaa atttatccgg cggcaattaa
    37681 cacaaaccgc acaaataatc aagttttaat aacttttatc ggaaaataaa tattttgacg
    37741 cctcgataaa ctcaggttga atattccgcg ttgattttga catattcgtg cgaaagatcg
    37801 gatgtgtaga cataatagga tttgtcgcct gctcccagat ccacttcaat atcgatgcga
    37861 tctttcttaa aggcgtcctt cagtctgctc atcagcacct cctggggcgt gttttgcgaa
    37921 aaaaccgcga gatcggcgat ggtcaggacg actttctcct tgtccatgga tatgcccgaa
    37981 gcgccgaggg ctgcaatgat tcttccccag ttgggatcct ccccgaaaca ggctgtttta
    38041 accagattcg aattggaaat cgcataagca gcgcagcgcg cgtcggattt cgatctggcg
    38101 cctttgacca tgatggaaat gaattttgtc gcgccttccc catcctttac gaccgcctgg
    38161 cagagttcca tcaacacctc tgtgagcatg tcccggaata actgcagacg cgcctgaacc
    38221 ttttccaggg gattattccc ggccagaccg ttggccagaa taatcgccat gtcgttggtg
    38281 ctcatgcagc cgtcaacgct gatggaatta aacgtggcat tgatcgcctg atgaaatacc
    38341 gtattcaacg cccggtaatc aatcagggcg tccgtcatga catacgtcag cagggtggcc
    38401 atgttcggct caatcatacc ggcgcctttg gcgatgccgc aaatggtgat atcttttgcg
    38461 ccgatgactc ctttccggat ggcgatcttg ggatatttgt cggtggtcat catggctttt
    38521 tcagcatctt caattcccgt ttccctgagc cctttaacca gtttgccgat gccgccttcg
    38581 atttttttca ccggcagtct tttgccgatc acgcccgtcg accccagcag gatcagttct
    38641 tctctgatgg ccagttcccg ggacaatgca ccggaaacaa tcagagcgtc gcggattccc
    38701 tctttgcccg tcgcggcgtt ggcgcaaccg ctgttggtga tgatcgcctg ggcgatgcct
    38761 cttttcaccc ggtcttcatt gatcaaaagc ggcgccgcct taaaggtgtt ttttgtaaac
    38821 atcgccgccg cccgggcggg aacggtggag tagatcaggc ccagatcctt ctgatccttt
    38881 tttttaatgc caacggaaat accgccggcc agaaatcccg gtacgctaat ttttaatttg
    38941 ttcttgatca tgaaattagc tcctctcggt taaacggatg ctccgcagca ttttttatat
    39001 ttcttgccgc ttccgcacgg gcagggatcg tttcttccgg ttttcgggcc ttcatgcttg
    39061 accgtttttt tctccggcgt atcctcccct ctgctcatca cgtaatcctg tttttgtttc
    39121 tgccggatgg cctcgacttc ttcctctcgc tgaatccgga tcagacacag tttttccaga
    39181 acgtccattt ttatccgggc gatcatttcc atgaacatgt cgtacccttc gcgctgatat
    39241 tcccggaccg gatccttctg ggcatatccg cgcaaaccga ttccttctct taaatgatcc
    39301 atggccagca ggtgttcctt ccactggaca tcgatggcct gaagcatgat gactttcatc
    39361 aggtagttca tgagctccgg gccgaattct ttttccttgt cccgcagata tttctgcacg
    39421 ccctgaatga tccattcccg aatggctgat tgaccgtcac ccgtctgatc ggtattcgcc
    39481 agatcaaatc ttatgttgaa ttgtttgagc agggcatcgt ccagaccctt gaaatttatg
    39541 tcttccttct ttgtcttttc gtcgacaaac tcggacaaaa tattgtcgat gatttcctcg
    39601 atcatctcct ccgtgtccgc ccagaggttg tcgccgcgca gcactttctt gcgctgttca
    39661 tagataacct ttcgctgacg gttcatcacg tcgtcgtaat ccaggagatg tttccgaata
    39721 tcgaaattct gcccttccac gcgtttctgg gcattttcta tggcgcggga gatgtatttg
    39781 tgttcgatcg gctgatcctc ttcaatgccg attttgtcca tgattccgga aatacgctct
    39841 gcgccgaaga tgcgcaataa atcatcctcc agcgaaaggt aaaaccgcga cgaacctctg
    39901 tctccctggc gcccggagcg gcccctcaac tgattatcga tgcgccggct ttcgtgtctt
    39961 tcggtgccca gaatatgcag accgcccaac tccgccacgc cctcgcccag tttgatgtcc
    40021 gtcccgcgac cggccatgtt ggtggatatg gtaaccatgc ccgcctggcc cgcctgcgca
    40081 acgatctcgg cttctttctc gtgattttta gcgttcagca catgatgttt gatcccggcg
    40141 cgcagtaaat gtttgctcag catctcggat ttttcgatgg aaatcgttcc gatcaaaacc
    40201 ggccgccttt ccttgttcaa cgtcttaact tccccaatga ccgcgttgat tttttcccgt
    40261 tccgttttgt agatcagatc gttgtggtcc tcgcgaatca tcggcatgtt ggtaggaatc
    40321 accagaacgt cgaggttgta aattttcttg aactcggccg cttccgtgtc ggcggttccg
    40381 gtcatgccgg ccagtttttt atacatgcgg aaatagttct gaaaggtgat cgacgccagc
    40441 gtctgatttt cccgttcgat ttttacgttt tctttggcct ccagcgcctg atgcaatccg
    40501 tcgctgtagc gacggccggg catgactctt ccggtaaatt cgtcgacgat caccacctga
    40561 ccgtccttga ccagataatc cacatcccgc ttaaacaggg tatgggcgcg cagcgcctga
    40621 ttcacatgat gcacgatttc tatgttgcgc ggctcgtaaa gattctgcac attgagtaat
    40681 ttttccacat gtgcgacccc gtcttcagtc agaaccaccg tacggctctt ttcctcaata
    40741 gagtaatctt tgtccttttt taaccggggg ataatctggt tgattttata atatttgtcc
    40801 gttgattcct cggagggacc ggaaataatc agcggggtcc gggcttcgtc gatcagaata
    40861 ctgtccactt cgtcgacgat ggcataattg aattcgcgtt gcacataatc gtccagcgtg
    40921 aatttcatgt tgtcgcgcag atagtcaaaa ccgaattcat tattggtccc gtaggtaata
    40981 tcgcagtgat aggcttctct gcgttccggg tcatccagcc cgtgaatgat gacgccgacg
    41041 gtcagaccca gaaatttata aatcggcccc atccattccg catcgcggcg cgccagataa
    41101 tcgttaacgg tcaccacatg acagcccttt ccttcgaggg cattgaggta taacggcatg
    41161 gtggcggcaa gcgttttgcc ttcaccggtt ttcatttccg caattttgcc ttcgtgtaaa
    41221 gcgatgcctc cgataatctg cacatcaaac ggccgcatcc ccagcgtccg gcgcgaggct
    41281 tcccgcgccg tggcaaaggc ctctgtcaga atatcgtcca gggaaaatcc gtttttgagt
    41341 ttttccctga aataaggggt tttggctttt aattcctcat cgtttaaagc cgtcatggcc
    41401 ggttccaggt tgttgatctc tcttaaaagc aacgacaacc gtttcaattc ccggtcgttt
    41461 tttgtgccga atattttttt caatatattg tccagcattc aatcaatacc ctaaaattcc
    41521 atattttttt aaaaaaaaac cggccaatta accggtctat tcaatcagtg aatgtatcaa
    41581 tacaacttca ggtaaaatca gtcaatcatt atctcagata ttttctcgga tttacctcga
    41641 cattgttaac taaaacagca tagtgcaaat gtggaccggt actgcgtccc gtatcgccga
    41701 cataaccgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aattcggacg
    41821 tcacccatcc cttcaccggc cagagcgaag gggttgaagc cagaacggct ttctgttggc
    41881 ccaaataccc caatagttcg gtgaagcttc tctctctttc attggcttct tcatgaagct
    41941 tttcgaagtt tctgcgcatg cccgcgataa tttgttcatg gtctttatca accaaatcct
    42001 tcattctgac ttcttctgat ccgcccacgc ccaggggcat tttcttgctc aggttggttt
    42061 catgggccag aacccggatc tgtttgtcat actgcctcaa ggcttccagg cgatcggcaa
    42121 actgatctat tttcatgccc atatcctgca attgacggga ttgttctttc gtctgcgctc
    42181 tcaaccgggc cagttccgcc cggtcgcgct tgatgctggc ataatcataa atgacaaaca
    42241 gagcgatcag aacaacgacg atggcgccaa tgagaatatt acggatggca accttcggaa
    42301 cggatatttt tttgaccccg ctcttccggt tgggaataat cataaatgta aaaaaattat
    42361 cggacactgc ttctttcctg cgaaatttat tatgagatta tcgatcaagt gccattcaga
    42421 tgcactttgc tatcacagaa aaatatttaa agtcaagcat aacttcgtct gctgttatta
    42481 cttgattttt ttatagtttt accgtattct aggccgtcat ttccaaaata ttgaatgatt
    42541 ttatgaagat attttatccg ttaaaatttg tccttatatt ttcagtaatt atggcgtttt
    42601 catccgtttc cggatgcgcc ttttcctgtt tcaacacaaa atttgatgct gcggattttt
    42661 taatgtccag gcctcatcaa ccggacggtt cttcccaggt gcttcttgtg caacgctgcg
    42721 gtttctggat tttcacccga acaatcgttt atgtcatgga gcaacgtgaa ggaaactggt
    42781 cggcggtctc cccccccatg gacgccgtca ttggacgaaa cggttttgcg ccgccagggg
    42841 aaaaaagaga aggcgacgga aaaacccctt ccggcatttt ccccctggtc atggcgtttg
    42901 gttatgctga ctctgcaaac acgaaaatgc tctatcgcca aatcaaagaa gatgatatct
    42961 gggttgacga ttccgaagcg gcagattata accgctggac aaagcaatcc gtgacgcaat
    43021 cgacatcgta tgaaagaatg aaacgtgatg acgaccttta taaatacgga atcgtgatcg
    43081 cgcataacac caacccggtc gtccagggaa gaggcagcgc tattttcgtc catgtttgga
    43141 aggacaagag cgttcccacc gcaggttgcg tggcagtatc cgaagaaaat atcctcaggc
    43201 ttcttcactg gcttgatccg gcggcagcgc cgctgatcat catgggcctg aaataataat
    43261 ccgggagcag gataacatga aaatcaggtt tttactggca gtaacagttt cattttcttt
    43321 tttttatttt gcgtccgcgg cgcattgctc cgacctgaac aaaaagccgg accgttttgt
    43381 ggatattcaa acagtcattc caggcgcgct gcttgatatc cgttatcacg gtgcgcacaa
    43441 cttcctgggc gaacgggttg acggctatct ggccccgaaa tgttatctga cgaaagaagc
    43501 ggccgatgcg ctggcgctgg tccagaagga tcttgaggct tattcgctga ccctgaaaat
    43561 ctacgactgt tatcgcccgc aacgggcggt gcatcatttc gtccgctggg caaaggacat
    43621 cgaaaacacg aaaaccagaa aagaattcta tcctacggtg gacaaacgga atctgtttac
    43681 ggacgggttt attgccgacc ggtccagcca cagccgcggc agcaccgttg atctgactat
    43741 cgttttgttg tccgcgccgc cgcagcccga ttacaccccg ggacaaacct tatccgaatg
    43801 ctacctgcct tcgggaaaac gctttgcgga taacagcatc gatatgggca ccggatttga
    43861 ctgttttcat gaactgtcgc atacggcaaa cgcaaaaatc ggagaacaac aaaagatcaa
    43921 ccggctgctg ctcaaatcgc tgatggaaaa gcacggcttc aagaattacg atctggaatg
    43981 gtggcactac accctgaaaa acgaacctta ccccgacaca tattttgatt tcattatcga
    44041 gtgaaaacat gaatccgaca caaccatctt ttttaaaatc gtacggtttt tccattttac
    44101 taattgcttc gattgtcatc ggcgccgttt tgggctttgt ttttaaggcc gatgccgtac
    44161 gcctcaaacc cctgggcgat atcttcctga atctgatctt taccgcaatc gttccgctgg
    44221 tcttcttttc catagcctcc gccgtcgccg gtatgcaaaa tatccgacgg ctgggaaaaa
    44281 tcatgtctgc gatgattttt gtttttatcg caaccggcct gatcgcgtcg gcggtcatga
    44341 ttgccgccgt gatctttttc ccgccggccg caggcttgaa catcggtctg ggtaacggtg
    44401 ttgatgtcga acacctcaaa gcttccgacc agatcgtcaa ggctttcacc gtcggcgatt
    44461 tcacagagct tctttctaaa aagaacatgc tggccctgat tgttttttca gtattaaccg
    44521 gattggccgc ctctgtgacg ggtgagcgcg gaaaaccgtt tgcgaatttt ctgaattcgg
    44581 gaagcgccgt gatgatgaaa gtgatttcct ttatcatgta ttatgctccc gtcggcctct
    44641 gtgcctattt cgcttatttg gtcggcgttt tcgggccgca actgcttgac tcttacgcac
    44701 gggccatgat ggtttattac cccacggctt ttttgtattt tttcatcgct ttcagcctgt
    44761 acgcttttat cgccgccggt atcgatggca tgcgtcgctt ctggaaaaac atcattccgc
    44821 catctctgat tgccctggcc accggcagtt ccatggccgc cattcccgcc aatctggaag
    44881 ccgccgataa aaatggcgtt ccccgggata ttcgcgagat cgttatcccc gtcggcgcaa
    44941 ccatgcatat ggaaggctcg tgcctggccg ccgttttgaa gattgcgttt cttttcggcc
    45001 tcttcaatat gcctttttca ggtgccgaaa cccttttaac ggcttttggg attgctttgc
    45061 tgaccggcgt ggtggtcagc ggcattcccg gcggcggtac catcggcgaa ctgctgatta
    45121 tttcctttta tggtttcccg atggaagctt ttccgatcat tacgatgatt ggaacgcttg
    45181 ttgacgcacc ggcaacaatg cttaatgctg tcggcgacaa tgtttcgggt atgctggtgg
    45241 cacgcatcct cgacaagaaa aatccggttg cgccggattg aaaatacgct gatcaagccc
    45301 tctgaagagt tttctcccgc cccgggcctc ctcgttgcat ggcgaattca gacacgtcaa
    45361 aatcccactt tcgagggatt gatatttttt tacaatctga gtatatgcaa aattatattt
    45421 ttcattattg agagatgaat acaccgcttt tacaaaattc atcatcgtcc ggcggacgag
    45481 aatagcgtat cgcgatcgtg aggggaaaaa gtgacaaacg ataaggaata ccgcctgtca
    45541 gcatcgataa acgacggaat cattgaaatt attctttcag gcacggtaac aagaaccaat
    45601 gtttcgcggt tgcaaaagga gattgattcc ctgaggaatg aaaaaggcag tcggttgctg
    45661 atcgacgtgc gaacccttaa tgaaggctcg ggataccacg atgttcttta ttatatcaga
    45721 cgtcccgata aggcaacggg aaagacggct atcctggatg ttcccggcaa tgacagattg
    45781 gaaacttttt taaaaacagc caccctgaac acccatttgg aagcgagata tttcagcaac
    45841 cttgacctgg ccagaaattg gcttaaaaca taatgaaatc aagaagccct gctttccgtc
    45901 gcgctcggaa atgcattatt gctttcaaat tggcttgaca aaaattcaac acaacatgta
    45961 tttttttgac atgatgcgac gatgtcacaa atgcctcaat gaaatttcgg ataatgttta
    46021 taccggacga caatcccagt gccccgtctg cagcgcggat ttgcattgct gcctgaactg
    46081 ctctttttac gatgccggcg cgtataatga ctgccgcgaa ccgcaagcgg aacgcgtcct
    46141 ggataaagcc cgctccaatt tctgcgattt cttcagattc cggcaaacgg aaacgctatc
    46201 cgatgcaccc tctcaaaaca cccccggaga caaactggaa gcgctgttta aaaaaagctg
    46261 accgcgcttt ctttacagaa gagtttcagc attgattctg tcgagcacag gcctgatcgt
    46321 ttcaaaaacc tgcgatgagg taatcacctt cagacagaca ttgtctgtgc aggccgtctt
    46381 tcgatggttc gatgcactga cacacggcga gcaggccagt ccggcataaa tcggcgtggt
    46441 ccggcccagg gacccgtaaa gtgcaggcgt ttccggccca aagatgacgt aggtgggcat
    46501 ctcggtaatg gcggcaaaat gcggcggtcc ggaatcgttg gtcaccataa attccgagac
    46561 gctgtatagt tggggaagct gggcaaagga tactttgccg gcgaagttaa cacatcgctc
    46621 atcctgaacc tgttccttga ggcgctctgc ctcctcccgc tcacgcggat cgccggtaat
    46681 cagaacaacc gcatgggaac attgtcccag aatcattttt atcaggtgtc cgtaataggc
    46741 ctgcggccag cgccgctgaa caagcatttc actggcattg ggattgatta gaacaatttt
    46801 atgccgggaa ggatcgaaag catcaaagca tccgcgaatg atgtccagga ccgtcttttt
    46861 ctgggtatcc gtgatgtgca ctttggccat gctgatttct gcatcctgaa tcctggtttt
    46921 ggaatagggg atttcttctt tcggcgaaag cagggcattg actaaagcga tgaaattttt
    46981 cgcgatatgc atgtgcgggt tataggcgac tttatgcgac agaaaatcgc cgcgataaag
    47041 accttcattg aaataggcat gaaaacctgc cttgtttgaa gcaccgctga aaccggtcag
    47101 cagtgcggta aaacgggaaa acagttccaa atcgatgacc gtgtcgattt ttctctttct
    47161 cgtccaatgc aggaatttaa acgtgtccat cagaaaagac gaaagacggt cctcccggat
    47221 ggcgtaaata ttgtccggcg aaacggtttg cagtagatcg aggctgggct tgtttttttt
    47281 aaagatggca aaatgcaact gggcgccggt tgtgtcgcgc agttttctca ttgccggatc
    47341 cacaagaatg gcgctgccca tttccgacag ttctataaac aggacatttt tcggatcttt
    47401 ctttgtccgc ggggaaagcc atttcaccgc tttataaaac agggacccaa aaaaacacag
    47461 cgggacgcct gcataaaaat ctatccggcg cattgtatca accttcatag accgatccct
    47521 ttcacaacgg gtaagattcg gcatcaccat aaaaaaaatg attgaaactg tcaatcaatg
    47581 aatggaaggc caatcgtttt ttttcgaaat ccgtcggtca tttcacgaac gtccgccgcc
    47641 tgaatcatgc aaacatggtt tcagaatctc tgcatgtttt acttgagaaa attcatcatt
    47701 gcgtgttaat ttcaacccgg acaaaaaaca gggcatgacc cgggatcctt ttgatcgcga
    47761 cggcgctctt tttatgtctg gaaatccatt aaaaaaagag ttgatcaggc catgcgcaaa
    47821 atcatcgacc gctatatatt taaggaaatc gcgttccctt ttattctgat tctttgcgtc
    47881 ctgacgttcg ttttactgat ggggaaaatt cttcaaatca tggacttgtt catcaataaa
    47941 ggcgtcagca ttttttccat cgcaaaaatt ttcattttta tgctgcccta tttcatgctt
    48001 tttaccattc cgattgcgct tctgatctcc atcctcatcg cgatcggccg attatcctcc
    48061 gacaatgaaa tcacggcgct gaagacatcc ggcgtcagta tggccagaat atattatccg
    48121 gttgcggtcg cttccctgct cgcgtttgtt ttcacgctgc tggtcagtta tttcgtcgca
    48181 ccacagagca atttcgcgac caagcggctg cttttcgata tcgcacagca aaatgccagt
    48241 gtcggcatca aggaaaaagt ttttaatgac agctttaagg gctttattat ttataccaat
    48301 aaaatttccg aggggaatca cgctttggaa gaggtcatcg tttccgacaa acgcaccatc
    48361 ggcgaacaga acaccattat cgcaccccgg gccttcctgg tttccgaccc cgacaccatg
    48421 agggtgaagc ttcggatgga aaacggatct atccacacag tcagttccga tttaaaaaat
    48481 taccgtaaaa ttgattttca gagctatgaa atcaatctgg atttgtatgc cgccctggct
    48541 gaattcgata aagcgtcgaa agccgccaaa gacatgacca tgaccgaatt actgcaaaac
    48601 atgaaaaaac ccgggatgag cgaaaaagat gttcgggaat tgtccatcga agcccacaag
    48661 aaattttcca taccgctggc gtgcatcttt ttcggtctgc tggcgctgcc gctcggcatg
    48721 cgaagccagc gctccgtcaa ggccagagga tttgccgtcg gcctgatcac ggtttgcttg
    48781 tattatctgc tgcgcatcgg cggcgaagcg ctggctgaaa caggctacat ccccgtggcc
    48841 gttggcgtct ggattcccaa tatttcattt gctttcatcg gcattttcat gttttacata
    48901 accagcaaag agatttatct tttttaaaca tatttttatt gctcccggca cactggagac
    48961 tcatccgagg acgtcgacgt gaaacttctg gataaatata ttctgaaaga atttttaaag
    49021 ttcttcacga taacgtttct ggcgttcatt gcgctttttt tgatcgtcga ttttttcgaa
    49081 cgcatcagaa tgtttctgag ccatcaggcc acggcggcac aaatggcgtc gtactttatc
    49141 ttctccgttc ccatgattat atcctatgtg ttgcctccct cgatcctgct ggccacattg
    49201 atgaccttca gctccctgac gaaattcagc gaaatcaccg cgatgaaagc caacgggatc
    49261 aatatcttca gaatggctct gcccatcgtc atgttcgcct ctttcaccgc ggtgttttac
    49321 ttttatttta cggaactgat tacaccggcc gccgttcaaa aaacgaccta catcaaaaac
    49381 atcgaggtga aaaaacaaaa aacgcccggc ttctttaaac agaatgaaat ctggtaccgc
    49441 agcgacaatg ccatctataa tttcaaaatg tttgacatcg gaaaaaacat cctgcacggc
    49501 gtcatgatca cttatttaaa caaggacttt accctgatca gccgcattga cgcccgccgc
    49561 gccgaatgga aaaacggccg ctgggttttt catgatctgc ttacgacgcg cttcgatgat
    49621 aaccgtgccc cggttctgga acgctcaaaa gaaaaaatca tcccgcttcc ggaaaaaccg
    49681 gaagatttca aagtaatgca gaaggatgtt gaaaaaatgg ggtattttga actgcgccga
    49741 tatgtgcaaa agatacaatc ggaaggtttc gatgcccgca aatacaacgt cgatctgcac
    49801 ggcaagacgg cctttccttt tgtgattttg attatggttc tgattgccgt accctttgct
    49861 ctgcgctccg aacgaagcgg cggcgtcatg cagagcatcg gcataggcat ttttatcggt
    49921 ttttcctact ggatcgtcca tgccttctgc atgtctctgg gcaaatcaga aatgctgcct
    49981 gttgtgttgg ccgcctgggc ggcaaacatt cttttcggtg ctgccgccgc atttctttta
    50041 tacaaagtca atacctgagc gcaacgcgtt tattcttaaa gcatcccgga atttaatatc
    50101 tgtaatgatc cggtttaaaa gggccgtcca cggtaatgcc cagataatcg gcctgctttt
    50161 ttgtcagttt ggataatcgg gcatccagtc gttccagatg cagacgggcg acctcctcat
    50221 ccagcacctt gggtagcgta tatacgccta ttttatgttt tttagtggca agctcaatct
    50281 gggccaggca ttgattggta aagctgttgc tcatcacaaa actggggtga ccggttgcac
    50341 agccgagatt gaccagacga ccttcggcca gtacgatgat ggagcgtccc gatttcagcg
    50401 tccacttgtc cacctgcggc ttgatggttt cttttctgca gactttgctt gtttccagat
    50461 aatgcatgtc cacttcgctg tcgaaatggc cgatgttgca taccaccgcc tcgtttttca
    50521 tcatttccat gtgccggcct gtaatgacat cgcagcatcc ggttgcagaa acaaaaatgt
    50581 cgccgatcgg cgccacttcc tccatggtcc ggacctcgta tccttccatc gccgcctgca
    50641 aggcgcagat cggatcgatt tccgtaatga tgacccgggc gccgaatcct ctcatggact
    50701 gcgcacaccc cttgccgaca tcgccataac cgcagacgac aacaatcttt ccggatatca
    50761 tgatgtctgt gcctcgttta atcccgtcgg ccagcgattc gcggcaaccg taaaggttgt
    50821 cgaatttgga cttggtcacc gaatcattga cgttgatggc cgggaataaa agttctcccg
    50881 ccgcctgcat ctgataaagg cggtgaacgc ccgttgtcgt ttcttcggat acgcctttga
    50941 tggttgaggc gatcttctgc cattttttcg gattgcgccg gaatgatttt tccagacggt
    51001 ccaagatgat tttaaattct ttattgtcga tcttttgttt caacaacgcg ggatctcgtt
    51061 caactttcac accctggtga acaaagaggg tcgcatcgcc gccgtcgtcg acaatcagat
    51121 gcgggcccga tccgtccggc caggtcagag cctgctcggt acaccaccag tactcttcaa
    51181 gggattcgcc tttccaggca aaaacggccg ccgatccttt tttggcgatt gccgccgcgg
    51241 cgtgatcctg cgtcgaaaag atattgcagg acgcccagcg gatttccgca cccaatgtct
    51301 tgagggtttc aatgagcatg gcggtttgaa tggtcatgtg gagactgccc atgattttca
    51361 tgcccttgag cggtttcttc tgtccgtatt tttttcgcaa cgccatcaga ccgggcatct
    51421 cattttccgc cagtttgatt tcctttcttc cccagtccgc cagtgctatg tccgccactt
    51481 tatattttaa agcaggatcg atttccaaaa aagccatgtt tcctccaagt ttgatttcat
    51541 ttttttaagc agcacgttag tctgcatgat gttgcgtcaa atatacacat caataacaca
    51601 ggtcatgaat gtagtctaat atttttatgg ggattgaact tcagttttcg agacaaaaaa
    51661 tatccggcgg atatccatgg gcacattgat ccggtcagac accttcatac ggtcaatgtt
    51721 tcttcttgat cttgttttta ccgtcaacaa agacacaggt cggtttccag tttttcgttt
    51781 ccgcttcctc gaccaccaca taactggcaa tgatgatcag gtccccttta gccactttac
    51841 gggccgcagc gccgttgagg cagatgattc cggaatttcg ttttcccttg attgcatacg
    51901 tggtgaatct ctccccgttg tccacgtcat atatcgcaac tttctcataa ggcaaaatgg
    51961 atgctgcttc caagagtttt tcatcaatgg aaatgctgcc ttcgtaatgc agatcggcat
    52021 ccgtgaccgt tgcccggtga atttttgatt tcagcataaa tctctgcatg gtttaagtgt
    52081 ctccttaaaa aattcaatat gatattttca gcgcttcacc gaaaacatga ttgtcaatca
    52141 gtcgagtcga accgattctg acagccagag caataacggt ctgatctgtg atttttgcga
    52201 tatcttgcag gcgcgtcgtg tcacagattt tcacgtaatc gatttgtgta agaggtgttt
    52261 tttgaatcat ttttttcacg gcatcgataa tgatacgggg cgatgtctct tttttcgcgt
    52321 acagtttttg agccagttgg agagacttga ataaaaccaa tgcagaggtt ctttgttcgg
    52381 cggaaagata tttgttgcgc gagctcatgg ccagtccgtc cgcttccctg accgtgggat
    52441 acccgatgat ctccagatcc atgttcagat cacgcaccat gcgctggacg acgacaaatt
    52501 gttgaaaatc ctttttgccg aagacagcaa catgcggctt gataatattg aacaacttat
    52561 tgcatacggt ggtcacaccg cgaaaatggc ccggccggga tgctccgcac agattctgcg
    52621 tgactttttc aaccgccaca taggtctgat agtgcggagg atacattgcc cggttcgacg
    52681 ggtaaaagat gacatctacg cctacgtttt gcgccatctt catatctcga tcaaaattac
    52741 ggggatattt ggagaaatct tcctgaggcc cgaactgcgt cggattcaca taaatactga
    52801 caacaacaca gtccgcttgc ttgcgggctt ctctcatcaa tgcaagatga ccttcatgaa
    52861 agtagcccat ggtgggaaca catgcaattt tctttccctc caaacgcagg ttttcagaga
    52921 aagactgcat tttctgaact gactttacga cgttcatctt cgaataaaaa aagcctttca
    52981 tcgcaagacg aaaggcttta aaattttcga tgcattttcc ttccgtctca gtcctttcgg
    53041 atccaagcga atttagggtt tcattatcag tattcgtctt tttttgtcaa gaaaataaat
    53101 acggttccga ataagtcatg atatattcat acgttccgcg atgattattg aacaaccaga
    53161 atttgacccg gaacaatctt tgcatcaacg gacaggccat tcaactccat gattctggcc
    53221 gcattggtat tatttttttt ggcgatggtg tgcagattat cgtttttcgt gacaatatat
    53281 ttgtccggcc ccagctgatt gatatccttt gccgacaata tttttttatc gatttttgat
    53341 ttttgagcgg atgttctcag atttttttcg gaatcttcat tgccggaatc ctttccgtct
    53401 tccttttgag gaatcttcaa gcgctgacct gcgtaaattt gtcccgacga caggttattg
    53461 atcgctttga gttccgatac ggacaccgaa aaccggcagg ctatcgtaga aaggttttca
    53521 ccttttttaa ctttataacg acctatggac gttatttctt tttgaatttt ttttttacgg
    53581 tcaatccctt tgcatgccgt ttggccgacg caacaggaat gagcagtctt ctcccctgaa
    53641 tcagtttctt tttcggactt aatttattca aggatcgaat gctggaaatc gaaactttat
    53701 actttcgcga aatggagtaa agggtctcgc ctctcttgac acgatgcttg atgtaggtcc
    53761 tgcgtgttcg gtcggatgcc ttgacatacg tctggacgga cgcgatgctc ggcttctcac
    53821 tggacgggat ttcatgatac acaaaactga atttatccag catgcctccc ggaactttta
    53881 aatcatattc ccgatccggc gtcatcttat gcctcaattc ggggtttagc aggcacaaga
    53941 tatcttccgt cgtgtcgatt ctttcggcaa tatcgttaaa cttcatgatc ctgtttaccc
    54001 ggaccgtttc aaattcaatc ggcctggcgg tatggctcaa atcaaatccg tatttttgcg
    54061 gattgttgac gatatgcagc gtggccagga atcggggaac gtaacgcgct gtttcattcg
    54121 gcaattgcct gtacaaatcc cagaagctgt ccaggtaatt gagccgctgt cgtgaaatca
    54181 ccctcaggac cctgccctcc ccgcaattat acgcagccag cgccgtaagc cagtcgccga
    54241 acataccatg cagttctttc aaataagcga tggccgcctt ggtggatttt tgcacatcca
    54301 tccgttcatc aacccattca tcgcgattca gtccgaactt atacccggtc gaaggaatga
    54361 attgccataa cccgagtgcg cgggccgaag aataagcatt gattttaaag aggctttcaa
    54421 caagaggcag ccagaagaac tcttcaggaa tcccggaccg tttaagctct ttcacaattt
    54481 cagaacggta catgccggac cgctcatagg cggcaagaaa actgtcgcgc tcgacggtct
    54541 gaaaagaagt gatctccttt tgcacatcgc cgttcattaa caagggaatc tcgccgtttt
    54601 tcccctggag cacatcccgt ttggaggaat aaacagcgag aatgcgctgg gaaattaaaa
    54661 gacgcagatc gtctttttcc tgagcgattg ccgaatcgcc gttggcgtta agaattaacg
    54721 aataagcctc atccagtgta tccagggttt cttcgatatc tccgtttttc caaagtttgt
    54781 cggcctcacc caataactcc agtgcatttt ccatcatgtc ctgtttttca tcatcctcct
    54841 gaacatccgc caagggatcg ttttcctgcg gaaaatcaaa caggctgctt tcgtcgtcgt
    54901 tattcaacgc ggcgagcttt attttctggt ctattttttg tttcgtgatg tcgccggagg
    54961 actccggact ttttttgtca atagaatttt cagcatatcc gattaaaggg acgatgaaaa
    55021 gacagatgag agcaatcagg cctatcagac ggtttcttgt caatccggtc attcagaact
    55081 ccttattcat aaatacgaca cgcttctgca tatccttcca tcatggccgt accgtatggt
    55141 tcatctttat agcataattt ttcatttttc caaaaattaa atttcccttg gacaaaaacg
    55201 cgacgatcaa aggagaagtt gccgtgcact ggcccatccg attacgaggt cgttgtcgtc
    55261 atcatcagat aggcttgaat gaattcatca atgtctccgt ccagcacttt accggcgttg
    55321 ccgcattcga cattggtccg gtgatccttg accagtttgt agggatgcag aacataggac
    55381 cgtatctggc tgccccacgc gatgtctttt ttctgcttgt tttcctcatc gattttctga
    55441 ttttttaact gtagttccct ctcatagagc cgcgacttca aaaatttcat ggccatcgct
    55501 ttgttctgat gttgagaacg ttcgttttgg cactgcacaa caatccccgt cggcacgtgt
    55561 gtaattctta ccgccgaatc ggttttattg acatgctgcc cccccttgcc tccggcacga
    55621 aacgtatcga tgcgcaaatc atcgtcattg atttcaattt tgatatcgtc atcgacttcg
    55681 ggatatacaa acacggaggc aaacgatgta tgacgacgcg caccggcatc aaagggggaa
    55741 atacggacca gacgatgaat gccgatttcc gccttggcat atccgtacga acactctcct
    55801 tcaagcgtaa acgacacgct cttcacaccg gcctcatccc ccggcagata atcgatgatc
    55861 ctggccgcaa attttttctt ctccgcccaa cgcagataca tccgcaacag catttccgtc
    55921 cagtcctgcg cctccgttcc ccctgcgccg gcatgaatgg atacgatggc attcatggca
    55981 tcctgctcgc cgttgagcat catcttcagt tcttcctggg caacggctgc attcaactgt
    56041 tccagctctt catcgatatc ctgcaggaca tgctcgtctt tctctctgac ggccatttcc
    56101 gcaaggtttt caatgtcgct gatgtctttg ctgaatctgt cccatctttc cactttttcg
    56161 gacagatttg tcttcttctg gagaacggac cgggcataat cctgatcatt ccagaaatcc
    56221 ttttttaagg ataactcctc cagatctttt attttcattt tcaattccgg aacgtcaaag
    56281 acactccccg atatgctgaa ttcttctttt cagatcgctg atcgtttcca gaatgttttc
    56341 cgtggtcatt tttgactcct tcgtcttaaa ctgaaaataa aacagatgct caccatcata
    56401 aaacacccga tgatgaaaat atccccgact cgttcataca ccgtcggtat tgtgacaaat
    56461 tttgcatgcc ccttcaaggc ctcttcctga aagatacccg tttttgcaat aattcttccg
    56521 gcaggatcaa taattccgga aattccggta ttggccgctc tgaccaggta caggcgcatt
    56581 tccaccgctc tgaaaaccgc catggagaaa tgctgaaagg gcgccgacgt tgctccgaac
    56641 caggcgtcat tggtaatatt gaccagcagt tcggcggctt tatttttata ggctcgcgag
    56701 gcaaacggta aaatgccctc ataacagatc agaaccccta tttttttatc attcagggac
    56761 aggggatcaa atcccgcccc ggaactgaaa tccccgatac ctgacgtcag ccctttgaca
    56821 aagggcatga gatccctgaa cggaacatat tcgccatacg gtacgagatg gactttatca
    56881 tagcgtcctc tgacgtcacc ggagggagac agcagatagg cgctgttaaa aaaatcattg
    56941 ccgtatgctc cgggctcata actcaccgag ccgaacagaa gccagctttg agtttttaaa
    57001 gacaggtcaa tgatctgacg gcgaaaacgg ttgatgtttt gaaatttaaa cggaaccgcc
    57061 gtttccggcc agattatgag actgccccgg ccgggcttgt tttttaaaga tagccgttca
    57121 tagatatcca atgtctcctg cttgtaatcc tgatcccatt tgacggcctg gtcaatattg
    57181 ccctgtatca gcgacacgtc aatgcccgga gcgtttgtca ccgtcgcatc aatctctctc
    57241 agacggaaaa atccgtacac atagattccg gcaatcagaa caaaaaccat gccggcggca
    57301 acatattctt tttttgttct cctgtggatc acatccaaga gaacggcatt taccaaaaca
    57361 atgagaaagg acaaacccgg aacaccgaaa atatcagcaa cctgaatcag ataggtgttt
    57421 ccgtactgcg aataccccaa attctcccac ggaaaacctg tcagaataaa ggatttgcaa
    57481 aattccagag aaacccataa agccggcccg atgatgtaaa acggtacttt ctcacgaaag
    57541 gacacaagac atccggcaaa aaacgacagg taaagactca gatacgcggc cagaagcagc
    57601 atcagcgcaa ttccgatatg aagcggaaga tgcccgtagt ttacaataac gtgggcaatc
    57661 cagtaaatga tccccacata actgatgatc ccggtaataa aacccagaag aagtccatga
    57721 cggatggtcg cagcgttttt cagcgcgtaa aaaagcggca ccagggccac ccaggccaat
    57781 acaccggatc cgtacttcgg aaacgacaaa aacagcagga taccgctcaa cgcggcaaaa
    57841 aagatattct tccggaaaag attccgggaa atgtctgcca aagagacttc gggaaaatta
    57901 tttttcatcg tcgttgtcgg tttcaatctt ttttatcttg acctttttga tacttctttc
    57961 atcggccttg tctatggtaa cgtccagtcc ttcgatctgg aatttttctc cggaaaccgg
    58021 aatgcgcttt atcgcatcaa ggatcaaacc gcccaatgtt tcatatctgc cttttttcag
    58081 atcgatgttc aggttttctt ctattttttc aatctccatg cggccgtcaa atatgagaga
    58141 tccgtccggc gactgaatga tgcggtcatc ggtgacattg ccatcatgtt cgtcgcggat
    58201 ttctccgaca atttcctcca gcatgtcttc caaggtaacc agacccgccg tcccgccgta
    58261 ttcgtcgatg acgatagcca tatgcttttt attttccttc aattcataaa acaggaggtg
    58321 ggtgtttttt gtttccggaa tataatgagg cgtgctcaaa caggcaatca aatcttcctt
    58381 ggtcatcggc ttggaccaga acatgaggat gtccttgacg ttgaggatgc cgatgatatt
    58441 atcaatggta tcccggtaca cggggatccg cgtatgtctt gaggcaataa cctcatggat
    58501 gatgtcatct attgccgcct ccgtgctaac cgcgacaata tccgtgcgcg gaatcatgat
    58561 ttcgcgaacc agcgtgtggg aaaactcaag gatattctcg atcatctccc gggacgacgc
    58621 atcgaccaaa ccgcttttct gggcgacgga caacatggca ttgattttgt ttttgatata
    58681 tttttgttcg cgccagaaaa aaagtttgga aatgtttttt ttgatgttca aatatcttct
    58741 ccctgagtta taggccccgc aaatattccg gtctgagccg gctaaggtct ttttcgtgaa
    58801 tcacagagcc gatcattttc agattttttt ccttttcacg cggcaggtgc gctttgaccg
    58861 gcagataatt cgacgcatgg ttggatgtga aatagcagtc cgtaaaagat gatttttcga
    58921 tcatcaggta aagttcccgg ataaaaccga attgatcggg aagaacaaaa cgacctgcaa
    58981 gataatcctc atacatttct gtttcgggaa tcagcatcaa cgtcagcgcg ccgacatagt
    59041 ccggatccat atccgtcaga atttttgccg tatccagagc atggtcaata cttttttccc
    59101 tgccccccaa ccccaaaagc actgttacag aaagaataat gcccgcttct ttaatgcgac
    59161 ggccggcctg gatcatctgc tcacacgtta cacctttatg aatttttttc aatagttccg
    59221 cattgccggt ttcaacaccc agataaacaa ttttcagccc cagcgatttt aattctttga
    59281 gttcttcaac gctttttctc aaaatgcttt tcgcattggc ataggttccg atcctctcaa
    59341 tatcggacag gttttcgtta atgagctcaa gtatttcttt aagtttcggc tgcgggatga
    59401 tcagcgcatc gccgtcgcat aaaaacactc tgtcgacgtc ctcatattgt ctggcctcgt
    59461 caagatcttt tttaatgcgt tcaatcggtt taattttaaa tttcttttgc cggtaagtgc
    59521 cgcaaaacgt acatttatta tgtgaacatc ccgtggtcac ctgtaaaagc aaactgtaag
    59581 cttcgcttgg cggcctgatg ataaatccct cataatccat gatttaattt atacttcctt
    59641 cctgttccta ccgccttgac taatggaata gcagattaga gcctgatcag ggcagcaaat
    59701 gcattgtgat tggggcttta tatcagagca cggggaaaaa tcaagcagta tgcagaggaa
    59761 aaaagtttgt gtaattcaca atttcctgaa taaattatgg gatccccgat ataggatatt
    59821 tataacgtga acggcccgca caacggagga cttcggggcg gacacggaaa tcgacaatac
    59881 aggaaaaaac gggacggtca tgatccttcg gtgcgggctc atggctgcgt gcatgcggct
    59941 tcacatgatc tcttgatata ttttccattc attgccgact tttttaagct tcagcgtttt
    60001 tttaccctta tcgttcaata gggaagagct gtatgtctgg ataaaaaccg cctccgcgtg
    60061 atctccatcc gcggtaattt ttaaatcgtc aatgccgatc tttatatccg tgcttttttg
    60121 acgcacgccg gttttatatg cgatccactt ttccagcgtc atatttttcg atttaaaatc
    60181 ttcatcatag caactgcgat aggttgcgat gtctcccgac tgccaggccg ccagccactt
    60241 actgaccacc tgacgaatgt cttcatcgga agtaccggca ggcaaacttt gcggcggagt
    60301 tcccgcttca gccggctttt ccgcagggtc ggcggataaa atcgcagagg gcttctcagc
    60361 aagattcggt cctttacaaa ttacgttttt caactggtac gcaaacgacc cttcaacgat
    60421 atcatcccat ttccctatcg cacccttcgg ggaagaataa agaacgttgc ttttttcatc
    60481 gtaaaagata agggcgctgt ctttatattg attattccgg cagtcgacgt ccatctgcct
    60541 taaaatatag ctgatcaaat caaggctgtt atccggtttg ttcgtgttgc ggaatttcga
    60601 tatggttttt attttcccgt tttcgcttaa gacctgcttc atccataaac gaaagacatt
    60661 ttcatccact ttcacaatgg aatttttgtc ataatatgat tctcttgaag aattggactc
    60721 caggtagacc cattccctgg ctgcccatgc ctgatgggta ataagaaaac aaattgccgc
    60781 gctgatcacg cttgtaattt tccagtattt cataagccat gggatccggt catgcaaaga
    60841 tcaacacctc tgaacgcgac ctcttatcgg gttcagatat ccgcttgagc acgacattca
    60901 atttcctttc tgcacggtca tgggcggaat cttttcgtca tcaaaagatc atcaaaagat
    60961 catcaaaaga atatccgcct attcctgatg ttctcaatca agcgcattgt ttgacattgt
    61021 ccggttattt ttcaagaaag ctcatatcaa caatgcccgc accggtatcc acccgacttt
    61081 cttccggcca ctgcagcccc agggtgtgaa ccagattgcc caaaccgctc agaattctgt
    61141 acgcgctgta cattttatca tattgagcat tgattaaatc gctttttgcc gaaatttttt
    61201 cagcggacga atcgagaaca tcaaaaagac tacgtcttcc gatggaccat tgggaaataa
    61261 aagcctccgc cgctttatcc gtagagtcga catactgttc cagttccctg acgcgccgct
    61321 gatctgccaa atacgactca tacgacagcc tgacggactg aatggtctga agttcagttt
    61381 tcttggcgat ttcttccgct tcgttaatca gatacgatgt ttctttgatc cttgcctgat
    61441 taatgaatcc gttaaaaata ttgaaagaaa gcgtcgcatt gagctgtaaa taatcttcat
    61501 agtcataaga agcggccggc cggttcaaat cctctcccca ggtatatccc gcaccgacat
    61561 cgacggtggg ataggcaagc gcccgagccg ttttgtgttg ataatttctg gcttcaatat
    61621 ccgctttggc cgattttaac gtcggatgac tggccagagc aattctttca gcatcctgca
    61681 gcgtggccgg tatttcagcg taggccggtt ccggttttac caaattaacc ggcaggtatc
    61741 ccatcacggc ctgataatcc gttttggcat tctcgatgtt tgcctgggta acaatcagat
    61801 tggatttggc cagggccaaa cgggcggtaa tttgatccaa atcaactctg cggtcgacac
    61861 ccgcctgact tcttaacttg atttgatcgt aaaatcgctc atgaatcaga agattttctt
    61921 tcgccaggtt atcctgctca atggcccgca gataatttaa gtaaactctg cacgccagca
    61981 atgccgtatt ctccgaaacc ccctgcaatt gataggccgc agaaatcact ctggattttt
    62041 gacgttttgt ttccgcgaaa gtagcacctc cccggaatat attctggttg agtctgacgg
    62101 tcgctgatct cggatgaaat tcatcatcca gaagataagg atgatgttgg tcaatatagc
    62161 catccgacaa cgtggtctcg atcctgggga aaaaatcagc catggcctgc cggacctcct
    62221 ggtcccttgc cagtcgatta taggccacag aagtaacatc cggattattc tgaagcactg
    62281 attttactga atcgcgaagt gtttctcccc tgacgccggt cgttccggca aaaagcaaga
    62341 taacaac
//
LOCUS       DAONQZ010000008        57145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_593_length_57145_cov_16.712069, whole genome shotgun sequence.
ACCESSION   DAONQZ010000008 DAONQZ010000000
VERSION     DAONQZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 57145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57145
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_57145_cov_16.712069"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..780
                     /gene="rimP"
                     /locus_tag="PLR13_02825"
     CDS             307..780
                     /gene="rimP"
                     /locus_tag="PLR13_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQC18295.1"
                     /translation="MDFDVKEKIRLLAEPVVASEGMELIHVECIKMHTRWIIRLFLDK
                     EGGITLEDCTNISNQLGDIFDIREVVNGSYTLEVSSPGFDRPISRDQDFLKYRNSRVD
                     VKTGIKIDGVKNFHGVLLDYIEENGRKLILVEVSGKVYRIPRQEILKANLADEKK"
     gene            854..2137
                     /gene="nusA"
                     /locus_tag="PLR13_02830"
     CDS             854..2137
                     /gene="nusA"
                     /locus_tag="PLR13_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQC18296.1"
                     /translation="MLPELKRLIEQMGKDRGISKEIIVEALEAAMLTAASKKFGQNVE
                     IEAHYNDETGEVEVFQFKTVVDKVLHPETQISKEEARATLDEGAEPGDSLGMKIETSQ
                     FGRIAVQLAKQIIIQRVKDAECDNIYDEYKDRRGELVNGFVQRFEGGSIVVNLGRAEG
                     IVPPSEQIHREAYKRGERIRAYIVEVKKSPKGLQIILSRTHPSFLKALFEVEVPEISE
                     GLIDIINVAREPGKRGKIAVKSKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIIPY
                     SEDPAKYVSSALSPAKVNRIFIDDENRTMTVIVPDDQLSLAIGKNGQNVRLAVKLTGW
                     NIDMKSETMEQAQEEGKSGQNDLTKITGIGPAMAEKLFSKGIKSIAMVAATEPELLSQ
                     IPGIGEKTAQQWIETAGKILNEEIAGSKSDSSSEN"
     gene            2164..4797
                     /gene="infB"
                     /locus_tag="PLR13_02835"
     CDS             2164..4797
                     /gene="infB"
                     /locus_tag="PLR13_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQC18297.1"
                     /translation="MPKKRVYELAKELGMESKVLIARLEKIGISVKSASASLEEADAE
                     RAKKELTAGELREVVEQRIKTTVIRRRTVVTAVEKPPEEVPLEEAKPPEKEPAEDKLK
                     KAPPGKTEKVKKEKPGEEPAVPAETVSEKAVKEVVPPAAVGPAAAETKASLEQKAAPL
                     EKSAEEKAVKPETKEAPVAPVKPTIITKHKIIRPETGKAVPPKVPAAKPAEKISQPSA
                     EKPKKKGKYQVEVLIEEEKKIPTKKILEKKIEKRLKRHEEDQEVVFTKWREGKKAAPT
                     KMKKTEITVPKAIKRRIKIGETITVGELAKRMGIKVSEIINKLMSMGVMATINQAIDY
                     DTASIVAGEFSFQLESADVEFDESVLKAPSTPENLKPRAPVVTIMGHVDHGKTSLLDA
                     IRQTNVSDGETGGITQAIGAYHVHVNGRDIVFLDTPGHEAFTAMRARGAQVTDIVVLI
                     VAADDGVMNQTVEAINHARVAGVPIIVAINKIDKPGADPEKIKQALTEHNLLSEQWGG
                     DTIFCEVSAKKKINIEELLEMILLQADVMELKADPDRPVRGIIIESKLDKGRGPVATV
                     LIQEGTLRESDSFVSKTEFGRVRALINDQGRRIKEAGPSMPVEVIGFSSVPQTGAEFF
                     CVDDEKKARAIADYWTRKAREKELSSLSKITLEQLYQRIKEGAKEFNVIVKADVQGSI
                     EAISDALNKLSTDDIKLKIIHSSTGAISETDVMLASASQAIIIGFNVRPDARVVDVAQ
                     REGVEIKLYDIIYNVIADVRAAMEGLLEPEYREVVQGRAEVRELFKVPKVGTIAGCYV
                     TDGKIMRSNNLKLVRDSVVVFDGKILSLKRFKDDAREVLAGFECGIGIEGFNDIHPGD
                     VIEAYAIETLEKKLEPNADKR"
     gene            4867..5205
                     /locus_tag="PLR13_02840"
     CDS             4867..5205
                     /locus_tag="PLR13_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HQC18298.1"
                     /translation="MVVGYGIINLRIPESGSLKSKRSVLNRILKRAQNEFNVSIAETG
                     HLDNYKLAEISFAVVGNDSRYINGKADHLLRFIDHLQIAEIVNCKMEIMVVSSFLETA
                     DGDIQKYDEF"
     gene            5195..5557
                     /gene="rbfA"
                     /locus_tag="PLR13_02845"
     CDS             5195..5557
                     /gene="rbfA"
                     /locus_tag="PLR13_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQC18299.1"
                     /translation="MNFKRADRVAELIRADMSEIIMKDVKDPRLHSVTITAVKVSDDL
                     RNAKIYFVEMGKDECSVEIKAGLAQASGFIRRELGKKLQLRFVPELMFIHDTSFGYGN
                     RIEKLLAQIHSQENENDS"
     gene            5547..6497
                     /locus_tag="PLR13_02850"
     CDS             5547..6497
                     /locus_tag="PLR13_02850"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQC18300.1"
                     /translation="MIHDIIQAIHEGQNFLITAHVRLDGDALGSELAVYLMLKELGKK
                     AVVYNKDRTPERYQFLPATHNISHTLTNIEQYDTCIVLDCSDLSRVGDEAENIRKIKT
                     LINIDHHVSNNGFCALKMLDAKASSTGELVFRLMQDMRFKMSRDICTNLYAAIITDTG
                     SFRYSSTTKETFMAAGVLVGEGASPQRIAENIYESDSPARLKLMARALSTLSLDLESK
                     VGSMVVTQKDLLETGAAWEHTEGFVDIPRTVKGIEVSVLYTQRGEKNFKLSLRSKAKV
                     DVEKVAKRFGGGGHIHASSCWMDGDIETIKSQIIQAVREL"
     gene            6503..7438
                     /gene="truB"
                     /locus_tag="PLR13_02855"
     CDS             6503..7438
                     /gene="truB"
                     /locus_tag="PLR13_02855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQC18301.1"
                     /translation="MMNGVIVIDKPAGKTSHDVVLDVRRALGIKKAGHTGTLDPLATG
                     VLPVCVNEATKLAGFLAGDKKEYRATMLLGVTTDTLDIEGNITGQSDKVVSEEEIRSV
                     MMRMIGTINQVPPAYSAVKHCGKPLYKWARKGVFPDLKPREIKIFSLLVENISFPSVK
                     FRVACSKGTYIRSLCADIGEALGTGACLSGLRRLQSGIFSEAMAVNLNDYKFPEAKEK
                     LAEKILPMAELLPLLATIELEDQCAVKLRNGWQPSVEMLKEHDLPLLKAGDMIKFVSR
                     GNLLAVAEMLAPVSELAAFDGKRQAARILRVFNQP"
     gene            7496..7762
                     /gene="rpsO"
                     /locus_tag="PLR13_02860"
     CDS             7496..7762
                     /gene="rpsO"
                     /locus_tag="PLR13_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQC18302.1"
                     /translation="MLSSEKRKEVIDNYRLHEKDTGSPEVQIALLSARIEYLTEHFKA
                     HKKDHHSRRGLLKLVGQRRRLLNYIKENDIERYRTVIKRLNLRK"
     gene            7898..>10011
                     /locus_tag="PLR13_02865"
     CDS             7898..>10011
                     /locus_tag="PLR13_02865"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQC18303.1"
                     /translation="MSNVFSADFAGRNISLKANYVAAQADGAVLVYYGDTVVLVTAVS
                     LKSTREGVDFLPLTVDYQEMTFAAGKIPGGFFKREGRPNEREILTSRVIDRAIRPLFP
                     KGYFSETQLVATLLSVDKENDPDVASLIGASAALGISDIPFKGPIAGVRVGRINGEFV
                     ANMCPEKMKESEMSLFLVGRKVTPGTSGRPYDVELVMMEGEAKEVPEDIIVDAIKFGL
                     ESIRPVIDLQDQIQASIGKVKRPVEEPVPDEELVARVREEALPGLKEGYAMPRKLERY
                     SKLGDVRAEVIKNIGGDDAVLSKKVAGIIENLESRILRDMIIQDKKRIDGRSSVDIRP
                     ISSEAGVLPRAHGSALFSRGETQALAALTLGTSADEQRMDYVGGEEIRTFLLHYNFPP
                     FSVGEAKMLRSPGRREIGHGALARKALVPVLPSPEEFPYTIRIVSEILSSNGSSSMAT
                     VCGGILCLMDGGVPVKDIVAGIAMGLLKEGNEIVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQMDIKIDGLTEDILRKALAQAREGRMHIIGKIRETLAAPRGDISQYAPRITTV
                     KVKEDQVRTVIGSGGKNIRQIISETGVTIDVEDDGTVTIASADAEAAARAVAMVKWLT
                     EEAEIGKIYRGTVKKIVDFGAFVEILPGTEGLLHISQIAKERIAKVTDVLQEGDEVMV
                     KVLEIDKSGKIRLSRKEALGAEV"
     assembly_gap    10012..10111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10298..10741
                     /gene="dut"
                     /locus_tag="PLR13_02870"
     CDS             10298..10741
                     /gene="dut"
                     /locus_tag="PLR13_02870"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQC18304.1"
                     /translation="MKKVKVCIQRLPGNDDIPLPQYMTDQAAGMDIFSAVAEEEIILP
                     GQRKKIATGIAVALPDGYEAQIRPRSGLALKNGVTLLNTPGTIDADYRGEIGLIVINH
                     GEEPFIVTRGMRLAQMVVQRVCRVDWEETETLAKTVRGSGGFGHT"
     gene            10820..11989
                     /locus_tag="PLR13_02875"
     CDS             10820..11989
                     /locus_tag="PLR13_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HQC18305.1"
                     /translation="MKKFKVKKTIKEINEKIKEGRAVVVTAEEMIDVVERHGDVEAAR
                     KIDVVTTGTFGPMCSSGAFLNFGHTSPKIRASRVWLNDVAAYGGIAAVDCYIGATEVA
                     ENDPLNSVYPGEFNYGGGHVIEDLVSGNFVKLRAEGYGTDCYPARKFERNITIKDLRD
                     AILFNPRNAYQNYNCAVNLSDKTIYTYMGMLRPQMANAAYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGFVAWPGTQHNPKVLRGSNGVPREGAGTIAVIGNLKEMSADWLTGA
                     SILGYGVSLFVGIGIPIPVLDEEMARYTAVRDEDIYTQVYDYSMDYPKGSPKPLAEVS
                     YKDLRSGTIVLEGKKVITAPLSSYFKARQIAYTLKEWIECGRFILGEPQQNLPSL"
     gene            complement(12081..13421)
                     /gene="holA"
                     /locus_tag="PLR13_02880"
     CDS             complement(12081..13421)
                     /gene="holA"
                     /locus_tag="PLR13_02880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQC18306.1"
                     /translation="MEKTLTNLKKGIVAPCYLLYGEEEYLIKEALNKMIAVLVPEADR
                     DFSLFYLDGENTEINSLMDHLLTPSLLGDTKVIVVQDTTIFTSRDDLAKLAAKIRTTI
                     NENPTKAARYFLTFLNIAGFSLEDLYGTGWQKITDEQWSGLVKGDAGDEREKWLPRIL
                     DICQTAGLTDASTSKTEKLEEVLKAGLPAGNCLIFTAEAVDKRKSLYKIVANIGVVNY
                     FSPVKKEYARKEILQKEAQKLLDGCGKKMAPAAWIALGKKTGFDFRNSLSELEKLISF
                     VGERALIDKEDVEEAVGRTREEEIFALTNALSEKNQLAAISTLNHLLDQGIHQLMIMT
                     MLSREIRLLLQARVMVDSGKLPKFNQSMEYGWFQNTVYPVFSALNPTGRKNDILILNQ
                     HPFSVFNALRNCVRFTTSRLTDLLDELLSLERSMKTSASNPQLLLENFLIKFCA"
     gene            complement(13446..14099)
                     /locus_tag="PLR13_02885"
     CDS             complement(13446..14099)
                     /locus_tag="PLR13_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQC18307.1"
                     /translation="MDEKQYVIDALSVNEAWRVFRIMAEFVEGIETLSNVDNAVTIFG
                     SARVKPDDIYYQKTEKLAKLLVGERFNVITGGGPGIMEAANKGAAEAGGKSVGLNIKL
                     PFEQKPNDYANIHIDYKYFFVRKVMFVKYAVAYVIMPGGFGTMDELFEALTLIQTKKI
                     KSFPLILMGSEYWKGLIDWLNNAMLAEDKILPVDMELIQIVDEPEDVVKLIKKYVIV"
     gene            complement(14157..14348)
                     /gene="rpsU"
                     /locus_tag="PLR13_02890"
     CDS             complement(14157..14348)
                     /gene="rpsU"
                     /locus_tag="PLR13_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQC18308.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKQK
                     RKTIEAQRRLAKVQRRRRY"
     gene            complement(14760..15824)
                     /gene="hisC"
                     /locus_tag="PLR13_02895"
     CDS             complement(14760..15824)
                     /gene="hisC"
                     /locus_tag="PLR13_02895"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQC18309.1"
                     /translation="MIIRNLIAKAILTQNGYSAPEITDAIKLDANENPFTLQEPLKRR
                     LADTLRSIDFNRYPAAGAPDLRERFADYYGVKKNMIMPGNGSDELIQILCMAFRGKIK
                     GVLVPSPTFSMYKIIAVNTGNKVVDVPLDQKFDLDVDAIAARIKNNFPALIFLSSPNS
                     PTGNLFSRERIEAVIRKAPGVVVIDEAYSAFAGQSLLPLLKKYKNVVFLKTLSKLGMA
                     SIRLGFLIGNPDLIDQLDKVRLPYNVNALSQKAAGFFLDHEGEFIKQIKEIIQRRQEL
                     YREIKKIKWIVPYPSRANFIFFSCAFDANRIYKDLVSQGIIVKTLNLAPRMPDGIRVT
                     VGSKKENQAFLDALKNITPE"
     gene            complement(15831..16655)
                     /locus_tag="PLR13_02900"
     CDS             complement(15831..16655)
                     /locus_tag="PLR13_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HQC18310.1"
                     /translation="MGWRFLNYQTNNIFENMAIDEAIFRGVITSKTESTIRFYGSEPE
                     AVSIGYFQDAKKDLNLGQCRHDGIDVVRRITGGRAVFHCNEITYSVTAGSGERIFPDN
                     ISGTYRVISKCLVRGLGNLGIDARLAETGDERNQGEKVACCFSTSAENEILVNGRKIC
                     GSAQVRRRGGFLQHGLLFYRFDPEGTANLLLSSRSPEQIEKLKQSVTSINEYLCQPLT
                     AEEICETLKKSFCDELGIHLGKGSLTPAEEKLKNELMKKYTDGLWNIEREKYFKAV"
     gene            complement(16665..17060)
                     /gene="gcvH"
                     /locus_tag="PLR13_02905"
     CDS             complement(16665..17060)
                     /gene="gcvH"
                     /locus_tag="PLR13_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQC18311.1"
                     /translation="MKDFPEDIIYSCEHIWVKVDGNMATIGITDYAQERLGDILSVEF
                     IETDTYVERDETFGSIESVKEVVDLISPVSGTVVVVNEDILEDVSIINRDPYDTGWLV
                     VIEMDNLEQLEDLLAASNYHDFIAQEESE"
     gene            complement(17080..18144)
                     /locus_tag="PLR13_02910"
     CDS             complement(17080..18144)
                     /locus_tag="PLR13_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQC18312.1"
                     /translation="MPQRLSGILEKMSVCKIDGLIICNMSTVRYLCGFTGSEGLLFVG
                     PKKPILFVDGRYTTQAGMETEGIKIIECADRIEAVALMTRQLKLKHIGFEADFVTWSL
                     HNKLTAQAPETKFIPWTDELKTLRAIKDDDEIMIMKKAAEISSAAIQSVVNRITPGWT
                     EKATAWQLEIAARENGADGIAFETIVASGENSSLPHAKPGKRKIRKGDFVVIDFGVRY
                     QGYCSDETCTLAFGKLTDRQKNAYQVVKDAHDRALELLRADIPGAAIDQHVRSVFGKK
                     YVRYFTHGTGHGVGLEVHEAPRLSSLSDDILKPRMVFTVEPGLYLPGRWGVRIEDTVL
                     LKENSCEKLTKMDKRLTIIE"
     gene            complement(18155..18775)
                     /locus_tag="PLR13_02915"
     CDS             complement(18155..18775)
                     /locus_tag="PLR13_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18313.1"
                     /translation="MREGNFGEALLIAENHLLDCPADADALGICCEALINMGRLEDMR
                     NILLQTEECISALNLVCERSGDACREKGFHQEAAACYERFISLRPETEKASEIIEKMT
                     LLDQEDHPTVPEKGKRNIPAQDFFTVTLAQLYIDQGHLQNAKTILDEILKKDPDNRQA
                     LSMLNNLISAPAASGTVNGNTKKNDEMIKILSSWLKNIERLKMNAA"
     gene            complement(18933..19592)
                     /locus_tag="PLR13_02920"
     CDS             complement(18933..19592)
                     /locus_tag="PLR13_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18314.1"
                     /translation="MNKGISFLQIGQFNSALKELLEAEKYYSRNPKVHYYLGIAYHGK
                     GMREQAIEEFEKAVSLDKHYSEAHNYLGTLYSEIQLYDKAIREFEKALGNHLYDTPSM
                     ALYNMAWAYYSKKDYKTALDKYQDVLRTDPMTGLRPQVEKNIGLIHYEQNNDMEAIRH
                     LKKSTELDPSLVDAYFILGQSYLRNKDSKNARRAFENVVKLSPDSSFGLKARNYLQSL
                     Q"
     gene            complement(19795..21516)
                     /locus_tag="PLR13_02925"
     CDS             complement(19795..21516)
                     /locus_tag="PLR13_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HQC18315.1"
                     /translation="MKKYFPKIFAAFVIITWIFTCIMGNSFAAFNASEENTAGITMLK
                     SIMLAKLPGKERIHLVVSKQPLIDVKSKDNGSYLIRLEDTDIPESLCRPLGEGELNNI
                     ISIVPSRQQLQGKNWVYLTIYPHEAVPYSIRQEGENVLIDFNVSGIEERKARTISKAS
                     ASKKPLAKVSAEPEKTDDFKKEDLDVSVKKSPAKKYMDRIVSLDFQDADIKSVLRLMA
                     EYGNVSIVTSEEVKGNITLSMKNVPWTKALETILEVNGLTKKQEDNVIIVTSMERSKR
                     EETAREEVRAREQKMMAEKGLLKQVLIEAKIVEATEEFVRNLGVQWGFGTVQRQHIGS
                     SQYGLGASGGSNVATGNQQALTYPSQIGIRDADGNPLAMAAVNLPTSLASPAIGLIFG
                     GASGFLETQLAALETNSTGKIISAPKVVTMEGIKAIIKQGDEVPYITPASGTSPATVS
                     FKEALLKLEVTPKLTDEGKISMDIKASNDTPDWSKAALNPQGNPPIVKSEVESKVVIN
                     DGDTVVVGGILRSTESKAVSGWPWLQKIPVLGWLFKTDSTKRDKRQLLIFVTPKILNN
                     DFTAEGT"
     gene            complement(21531..22220)
                     /locus_tag="PLR13_02930"
     CDS             complement(21531..22220)
                     /locus_tag="PLR13_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HQC18316.1"
                     /translation="MKKIFLIFINCSLILLTTAFFAFAAEAQNTASPAPKSVAPVKGK
                     VQPAPAGTVVPGPARTALSPAAAKTPVPAPAKQAPAVSPAQQTTVSSAANATATPTVQ
                     TPMPAPTVPNQADTYSYNPAGKPDPFRPFIVIETVKKTKPEEKKIKPSIFPLQRAEAR
                     SYKVVGIVGNEDHRVAIAEDASKKFYPLLVGTRIGLHNGKVVDILPDRVIVEEYENNK
                     GNKVILKLHKN"
     gene            complement(22217..22939)
                     /gene="pilO"
                     /locus_tag="PLR13_02935"
     CDS             complement(22217..22939)
                     /gene="pilO"
                     /locus_tag="PLR13_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQC18317.1"
                     /translation="MAIDINTIKKIPPKTKVLIVIVGFLLIGYFYWFYFLSSAMEKRT
                     TLNTQLTEMQNKINEQKKIAMQLNKYKADVEALQASYKIALQKLPDQREIPSLFHSVA
                     SAGRDAGVEFVLFEPKASVPKSLESQGPAASKTAALLKPSDQRDQKPADSSEKKDGAA
                     PSTEPFYEEIPVGISVLGSYQNILYFFDKVSKLPRIVNVSDITIGDRKEIKDKGHLVT
                     GSCTIKTYMFIDKKEKTSEKTQ"
     gene            complement(22943..23491)
                     /locus_tag="PLR13_02940"
     CDS             complement(22943..23491)
                     /locus_tag="PLR13_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQC18318.1"
                     /translation="MIKINLLPYREKAKKDTFLRQIFIVAGSVVLFILLLAWVTIWIK
                     SSISNLETQIKDSEALITDLNKKIGDLEKFKKDKKELEQKIGVIATLEENRLTPLKTL
                     DNLAMLVPPKDIWLVRLTQKGNSLNIEGMGRDNIAVANFMKSIEQFEPVQSVDLVSSK
                     KTEISGNAVQQFNFACILKKGF"
     gene            complement(23488..24570)
                     /gene="pilM"
                     /locus_tag="PLR13_02945"
     CDS             complement(23488..24570)
                     /gene="pilM"
                     /locus_tag="PLR13_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HQC18319.1"
                     /translation="MVDLKEIFSGKKSLVGLDIGSSSVKLAEIISNAKGYYLNRFHEV
                     PLPKGIIIDGILADAPVLSSKIKELFKIAHCGRKGVVTSLAGNSVIIKKVTLEQMNET
                     ELRELIHDEAGKYLPFDNMDDVNYDFQILGENNYNPNQMDIVIVAAKKEIVSSYLEAV
                     TAAGQNVEIVDVAPFVLETLYEVNYEFDNEEIVVLINIGASTTGINVVKGGMSVFTRD
                     FALAGNSITESIQEKYQIPFEEAEKLKIEGFPEGSENDNLDLKNNILEFAQPICMEIE
                     RSIDYFRSTFGGEDIKQVLLAGGSSRIPNITKHLSELLNVKTEIMDPFLKINYNKRKI
                     DVKNLDSIKPIAATAIGLGLRKIGDK"
     gene            complement(24606..24833)
                     /locus_tag="PLR13_02950"
     CDS             complement(24606..24833)
                     /locus_tag="PLR13_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQC18320.1"
                     /translation="MSLLITFGNRGLVDNYLMSKRLAELKSQNSAITAENNALKKKII
                     LLRNDINYIEMIARNELGMVKKGDIVYRWAK"
     gene            complement(24930..25127)
                     /locus_tag="PLR13_02955"
     CDS             complement(24930..25127)
                     /locus_tag="PLR13_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQC18321.1"
                     /translation="MADNMKKNHVKKFRETIPLSISELARRAGLTPQTISKMEKGLST
                     RKNSELKVAKALQRPHEEVFP"
     gene            complement(25378..25947)
                     /locus_tag="PLR13_02960"
     CDS             complement(25378..25947)
                     /locus_tag="PLR13_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18322.1"
                     /translation="MSETTSDWFNKAQALSDGEKFSDPQKAIEYLNNAIRLQPNDAEA
                     YYNRGIAYDNLGRHQQAIKDYSQTVRLKPDYAEAFHNRGTLYNTTGQYQLAIADFNES
                     IRINPGEADNYIGLGFAYDQLGQHQQAITEYSKAIALKPDNARAYNNRGADYLSLGNH
                     QYGCLDAKKACELGNCKLHKIAEKQGVCR"
     gene            complement(26045..26713)
                     /locus_tag="PLR13_02965"
     CDS             complement(26045..26713)
                     /locus_tag="PLR13_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQC18323.1"
                     /translation="MDLIEAIQHRRSIRAFTDQPVPKETIMEILNIACRAPSAMNTQP
                     WEFIVVSGEPLNKLRAAIVDKLTSGAPITPDHQVVGWPDKSVYKDRQITLAKQLFKAM
                     DIAREDKEKRAWWMERGFRFFDAPAAIIIVSDKILGDHASLMDLGAVMQNICLTALHF
                     GVGTCIEDQGVMYPEVVRHCTGISSNKRLMIAIAVGYPDETFPANHVRSEREPAENLT
                     AWVE"
     gene            complement(26857..26954)
                     /locus_tag="PLR13_02970"
     tRNA            complement(26857..26954)
                     /locus_tag="PLR13_02970"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26917..26919),aa:Sec,seq:tca)
     gene            complement(27035..28273)
                     /locus_tag="PLR13_02975"
     CDS             complement(27035..28273)
                     /locus_tag="PLR13_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQC18324.1"
                     /translation="MIPVEKALKTILVNFQPLGLETVHLLKARGRVIGENIMSPRNIP
                     SADNSAMDGYAVRCADTRGAGKTTPVKLKIIEDIPAGKFPRKNIGKNETARIMTGAVI
                     PPGADAVIRQEDTRKDGRTVIIFASAARGENIRFAGEDVRKGESVIPKNSLLRPAHIG
                     MLAALGKSSLKVYQKPRVAIMSTGDELVSIRTNPPPGKIVNSNSYSLAAQVAECGAIP
                     LILAIARDKKSDLEKKFKKALNADVIISSGGVSVGDFDFVKNVMGEIGNAMHFWQVAM
                     RPGKPLAFGAIEGVPLFGLPGNPVSAMVSFEQFVRPSLLKMQGHKKIFRQTVTAICHE
                     EFEKAAGFRHFIRAVVRKEKDKYIAVTTGDQGSGILKSMVTANGLIVMGENETRIKKN
                     SQVTVQLLDDSLSWSDALQL"
     gene            28533..32045
                     /gene="mfd"
                     /locus_tag="PLR13_02980"
     CDS             28533..32045
                     /gene="mfd"
                     /locus_tag="PLR13_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQC18325.1"
                     /translation="MESFRLLLKQIDDGMMRIAVQGLAGAARSFLISLLYKKRQRPVL
                     VVCPEEKEAVAFARDLSLFLGEEHVFHYPALDFLTIDMFALQRDEELSRLEVLANLQI
                     QHKIIVVASANALMQKVMPVAEFSKHLRIIAPGDTINRDEFAAQLVAQCYKRESLVQE
                     KGEFSIRGNIIDIFPPTEKNPLRLEMLGDEIESIRTFNTSSQRSIGPLPAFVLSPAGE
                     AVMDHSTLELAVRNIRRRADELSFSRDIRSRLVAAVQEGLTNAFNPIFLPLFYESYDT
                     GSGLSDNTLSGLLEYLSRNTLVVLDDPLAVGHAMFNAGLAMDKLLFKAKSGGKFYLEK
                     ENTYFPPEAVTAGLDGFLQVALAGLEIESERYHVPVVFESDRFTCTGEPLAGEIREDA
                     TLRLTVEKIKSWFAGDMLVFLFCPLPEDVQRMKGLLSSYDLPAETLSGAEPVLDSLEG
                     WKGDARLILFVGDISSGFVMPAIKLAVLSEEEIFVKKARRLRSRPVRTGYFIKSFGDL
                     QEGDFVVHTDFGIGIYRGLKKIAVRKIENDFLVIEYSEGDKLYIPVNALEKIQRYVGP
                     DGYVPKIDRMGGTSWEAVKERVKKSVRDYAEELVSIYAARETLERKSFAPPDRIYEEF
                     CSTFEFEETPDQVKAIEDIHQDMDDVKPMDRLICGDAGFGKTEVAIRSAFRAVMDGKQ
                     VAVLTPTTILAEQHYQTFTRRFEDFPVRVGLLNRFKKPAEQKKIVEELKNQTIDIVVG
                     THRLLQKDVAFQDLGLVIIDEEQRFGVAHKEKLKKMRTLVDVLTLSATPIPRTLHLSL
                     VGIRDLSVINTPPKDRVSIKTYVMEFDEDAIKTAVEKELARQGQVFFVHDRVRSIYAI
                     ARLVQRLVPHAKVGVVHGQMKPAEIEKTMAGFIRQENNVLVCTTIIGSGLDISTANTI
                     IINRAEKFGLAQLYQIRGRVGRSSREAFAYLLLPQGAILSRAAMKRLQVIKEFSDPGS
                     GFRIAYNDLEIRGGGSVLGMSQSGHISAVGYELYTELIEKTIREVKGEKTEEDWMPEI
                     QLGISAFIPEDYVPDVHQRLILYKRISLAANDEDIRAIENELLDCYGEMPRVADNLLR
                     VISIRNVLKPLKGKKMGYDGKYLYIFFRENSPVDPGKIIALYRKKIKELRFTPDHKLF
                     VPAPAAADMDILVQADMLLKMLAQ"
     gene            32069..33076
                     /locus_tag="PLR13_02985"
     CDS             32069..33076
                     /locus_tag="PLR13_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQC18326.1"
                     /translation="MMIPTRWFSKFYSLAGKSKKVSPVRCGLLCLLLPGLMIACDKME
                     SFSRPHVATVNGAKIYLDEYQSLLNEKARMLSGDFFRQPAYVKKFEDEVLDAMIGEKI
                     MLLRARELNLSVSDAELEAKIKEIEKDYGNEFTGLFARQNINYEKWKEDFRKEILLQK
                     LIAADVHSKIIVSETEIEDYFKKHRHIYKRDLSARVSQIVVRDMQTAEQAMQRLKSKE
                     EFARVASEMSIGPEASRGGDLGFITKRVMPEPLDETIFKIPVQTISPIVQSSYGFHII
                     KVMEVRQAKERILPEAREEVIADIRLLKEKTAFAEWLEALKKKAVVQKKADIKINTSY
                     R"
     gene            33098..34072
                     /locus_tag="PLR13_02990"
     CDS             33098..34072
                     /locus_tag="PLR13_02990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQC18327.1"
                     /translation="MKFYGKYVFALLGIMLLCPLLHAEVVDKIIAVVNDEVITRLELN
                     AAFEPYRKNIENTYQGGDKDQFVLHAREEFLNRLIDNILIEQEAKKTGISASVREEDV
                     MGVLQDMLTKQNISMQDFLKNLAKDGSSIEEVKKEIRSQMIRMRLLRREVKDKIIVTD
                     EEIGAYYNQHRNDYEGKESVRMKQLLLLLPSGANAETKGKIRKEALHLRERIMAGESF
                     DLLVSTYSQGPAAAQGGDVGFIEKGTIIPEVEAVAFSLAVDQVSNVIESDAGFHIIKV
                     VDKRGAGLKPVAVVREEIKAKIEDEKLDKKFEEWISAIRAKSHIEIKN"
     gene            34086..36899
                     /gene="uvrA"
                     /locus_tag="PLR13_02995"
     CDS             34086..36899
                     /gene="uvrA"
                     /locus_tag="PLR13_02995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQC18328.1"
                     /translation="MDAIKIRGAAQHNLKNISLDIPRDQFVIITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFIGQMDKPNVESIDGLSPAIAIEQRTASHNPRSTVGTVT
                     EIYDYFRLLYAAIGVCHCYRCGREIKSQTIDGILENVLALPENTPFSVLAPMVRGKKG
                     EFQKELKKLQKEGFARVRIDGENRDLAEDISLEKNKRHDIDLVIDRLVLKNGIRKRLR
                     DSLEIALKHAEGLVRIVTAGGEEFLFSEKYACPDCGISLPALTPRMFSFNNPYGACPA
                     CNGLGSSMRFTEYLIVPDAGLSLRDGAIAPWAGRHSLYYYSILDALSSHYGFDVNTPF
                     NKLPEKIRKVLLYGSGSETIKFYHDRGNKRYFVHRPFEGAIRQLERRWRETASTAIRN
                     DLMRYIDLSACETCHGARLKEEILAVTVMGKNIYELCKMSIRDCHSFFERIELSPQEL
                     NITERIIREIKSRLQFLLNVGMDYLNLERATSTLSGGESQRIRLATQIGSGLTGVLYV
                     LDEPTVGLHQKDNLRLIDTLKKLREMGNTVLVVEHDADMIMACDHIVDIGPGAGTEGG
                     EIIFQGTPVQILNSETSLTGRYLSKKESINLPSEKRPLTGRHIILEGASENNLKNIDI
                     KIPVGVFTAVTGVSGSGKSTMVIETLYKVLARRLNQYNGSMGKIKRVVDLGGIERVIM
                     INQHPIGRTPRSNPATYTGVFSFIRDLFTNLPESRMRGYKPGRFSFNVKGGRCEACEG
                     NGLIKIEMHFLPDVYVTCEACHGKRFNRDTLDIKYKDKSIADVLDMTVNQALTFFSNI
                     PSIRSHLQLLGDVGLGYIHLGQSATTLSGGEAQRIKLARELSKKVNSNTLYILDEPTI
                     GLHFADIQKLLDVLMRLVNMGNTMVVIEHNLDVIKCADYVIDLGPEGGSGGGRIISSG
                     TVEKVARDEKSVTGKFLRDVITA"
     gene            37097..38209
                     /locus_tag="PLR13_03000"
     CDS             37097..38209
                     /locus_tag="PLR13_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQC18329.1"
                     /translation="MIVDDEDSILNAFKRILADENYDVHVANSGLEGLNKLRSATKPY
                     SIIISDQRMPEMSGVQFFSKAKEIFPDAIRILLTGYADSDAIIEAINKGGVHLYFTKP
                     WHEEEILLHIKQSLSKVEILAENKRLVELIREKNKELTELNKALEQKALEKTNDLLIQ
                     TEKLKESYQKSQIIMDGIVRTLSKIIETRDPYTAGHEDQVAKIACKIAREMKLPEEQI
                     SFIHIAATLHDIGKICVPSEILTKPSALNNLEKAIIKTHCKVANDVLANIEFPYPVAE
                     VIYQHHERLDGSGYPRGLRGNEIALEARIIGVADVIDAMASYRPYRPALGVDAAIEEI
                     VKYRGITYDPDVVDACLTVYKKTARTLIETNHRTVN"
     gene            complement(38241..38933)
                     /locus_tag="PLR13_03005"
     CDS             complement(38241..38933)
                     /locus_tag="PLR13_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQC18330.1"
                     /translation="MGTDIAANIAAVKQRIAAAAERGKRSPDSVKLLAVTKTVPVPVI
                     EEAIRAGITALGENYVQEAREKIAVIGRSIPWHMIGHLQTNKAKYAVILFDYVHSVDR
                     LDLAAEIDKRAGRAGRQINILIEINVSGEKTKNGIPAGKAVALLQDLSAMQHIAVKGL
                     MTMAPYSDDPETSRPYFRELKNLQQNIIRAGIAGIHMEELSMGMTDDFDVAVEEGATL
                     VRIGRAIFGERN"
     gene            complement(38938..39531)
                     /locus_tag="PLR13_03010"
     CDS             complement(38938..39531)
                     /locus_tag="PLR13_03010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQC18331.1"
                     /translation="MSITAHASLILASASPRRQEMLRSAGVHVKIIPAHVNEKYLTGE
                     TPRQHVRRLSSLKAMKVAVENPDACVLGADTIVVIDGTVLGKPRNKTQAKTMLKKLSG
                     RQHTVFTGFTVVRLSFGIRSTGVVQSVVRFKTIHPDEMEWYIAGDEPYDKAGGYALQG
                     QGACFVRSIRGSYTNVIGLPLCEVLETLKKLHVIDFC"
     gene            complement(39958..41034)
                     /locus_tag="PLR13_03015"
     CDS             complement(39958..41034)
                     /locus_tag="PLR13_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQC18332.1"
                     /translation="MYAVIMAGGRGVRFWPRSREKRPKHLLDITGEKTIIQETVNRIK
                     PLVPPTNILVVTGKKHARALMKQLPEIPPRNILIEPVGRNTAACIGLAALHIRKRSTD
                     DTMMVLPSDHAIADSGKYLSVMKAAAVVAAQEKGLVTIGITPSSVQTGFGYIEGGILC
                     KTIKNQDVFRVKSIREKPDFRTAAKFVRSGNFYWNSGMFVWQTSVILEEIKRWLPELY
                     AGLTLIDTSLGTAGETATVSRVYKKLGSVSIDYGVMEKAGNVFILPGNFGWSDVGSWD
                     ALWDISPKDKQNNVILSGSRTVLEDSKGSLIYNPRKLTALLGVRDLMIVETPDALLIC
                     KKGRSQDVKKIVERLEAGQLKKYL"
     gene            41095..42330
                     /locus_tag="PLR13_03020"
     CDS             41095..42330
                     /locus_tag="PLR13_03020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQC18333.1"
                     /translation="MSCNFKAENIFYATMITINYPEIILKKAREDAILRGHPWIFSGA
                     IGSVKGNPGPGDIVLARDAAGHPLALGFFNPSTDIAFRVLTRRCDENISPYFWQSRLH
                     DAYKLRQRLINEQTNAYRLINAEGDGFPGLIVDVYNTTLVFSIAVAGMEKQKNHLLNA
                     LIAQLKPTRIYEQSDSHSRKLEGLENRSGIAFGEDENSTVEIMENGLKFEVDVVSGQK
                     TGFFLDQRVNREKIGSLGRDAHVLNCFAYTAAFSVYCAEGGAKKVVSLDISKTACVAA
                     RKNLHLNWFSVENYPVIETDVFDYFRHTPENYNMIILDPPAFAKTKRDVPKAARGYKD
                     INLQAIKHLSKGGMLATFSCSNFIEEDLFYKIVLGAARDAGAELQLLSRLDAGPDHPV
                     AFGHPEGRYLKGLLLVKRN"
     gene            complement(42354..42752)
                     /locus_tag="PLR13_03025"
     CDS             complement(42354..42752)
                     /locus_tag="PLR13_03025"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HQC18334.1"
                     /translation="MKNTITVFFFIMCLLLAGCGAAVNLQGKVAGISSGRFFYQDGHL
                     VSDYKADIDAVWQACEQSVTDLKAKSIRKERKISTGVITSVIQDEKVTIKVEYVGRDM
                     TSVSVFVGAFGNTMASRLIHDRIIGNLAKN"
     gene            complement(42825..43409)
                     /gene="pyrE"
                     /locus_tag="PLR13_03030"
     CDS             complement(42825..43409)
                     /gene="pyrE"
                     /locus_tag="PLR13_03030"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQC18335.1"
                     /translation="MTIDQMKERLGEIILERSFKYSDNPPFTLASGRQSNYYFNCKPT
                     TLDPEGMNLIGTIIFDMLKDTDITAAGGLTLGADPIANALAVISYQKGKPIKSFIVRK
                     DAKDHGTKSAIEGSVRPGEKVAIIDDVITTGGSTITAIEQARKAGLSVVMVITLVDRE
                     EGGRENIMKHMDNIASILTRTEIMNLRERKAAQR"
     gene            complement(43435..44157)
                     /locus_tag="PLR13_03035"
     CDS             complement(43435..44157)
                     /locus_tag="PLR13_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQC18336.1"
                     /translation="MKYRHSAHQQIALIWALAFSVLIHFSAAAVLIGFELSGGAPAPS
                     DRNVLNMVWVSFDNKKADTGKAVQTGQQTIRQKKNTGRMTSVERPASPVGTVQLPLAS
                     RTAPAVESTFSEYENRPEINKSSVEARHSGGFEAVAQHPMSDIAIAYPLYRENTPPVY
                     PEMARLRGYEGIVLVFAEILPTGRVGDVKIRKSSGYAILDQSAIQAVKPWKFEPAKKA
                     GNPFTAWVELPIKFMLQHHPQS"
     gene            complement(44173..46572)
                     /locus_tag="PLR13_03040"
     CDS             complement(44173..46572)
                     /locus_tag="PLR13_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQC18337.1"
                     /translation="MVEIINIRNFLKKKIMILDGATGTELQKLGLPAGVCPEIWCLDN
                     PDIIGAVHKSYQKAGAQIIYTCTFGANRYKLKQFGAKHDVYAVNLKLAQLARKACGNQ
                     TLVAGDIGPTGLFVEPFGTLAFEEAVDAFKEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELADVFTIASMTYEKNGHTLGGTPPQSALITLQSLGADAVGCNCSAGPEQMID
                     FISAMKPYATVPLVAKPNAGMPLLKDGQTVFEMDPATFADFSVKLAAAGANMLGGCCG
                     TTPEHIRALRMSVTRKKPVLPLTKSVCALSSARRFLEIKDNDPLYIVGERINPTGKKS
                     LQQELLEGKLSIIRQMALDQENQGAVLLDINVGQPGIDEVQTIRNVISLLSTVSSLPL
                     VIDSSRIETIEAALRLYPGRMMINSISGEKEKLARLLPLAARYGAMFILLPLTGGEIP
                     RTAKKRRVVIDRIFRKAQAFGFTKKDFVVDGLTMAIASDADAARETLETIQWCKNIFQ
                     CKTILGLSNVSFGMPGRPWINAAFLAMAQHCGLTMAIANPASVEFMNVKNAGDALTAR
                     ERAAFHFINYFSQQTPAVAGLPSTAVSAGEKIVQAILEGNKDDITALLDEAMKSGKTA
                     SQLVDDYMIPSIVRVGDLYEKKIYFLPQLMAAAEAMKKALSHLDPYLQKATITTRGTV
                     LMATVRGDVHDIGKNIVALLLRNYGYRVIDLGKDVSEQAILDAAKKEKPDVIGLSALM
                     TTTMVNMKEVIALARAQGIRTDFLIGGAVVTDQYAKSIGAFYARDGVQAVKVVEKIIK
                     P"
     gene            complement(46675..49293)
                     /locus_tag="PLR13_03045"
     CDS             complement(46675..49293)
                     /locus_tag="PLR13_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18338.1"
                     /translation="MKKNNAPPTGNPQPLQVLMLNDSDKDVSRIVSELQKGGYHPVYE
                     RIETAAAMRQALRDKPWDIILCDSNYLKFGAVAAMALLEEEKTDIPLIVISGASSKDK
                     TIECRFPGTRNYIVKENYSRLCPVIARELNETKIQAKHKQTESQKEAAQEELRRSEER
                     YRKITTCIPDLIWTMDLAGHFTYVNEVVERTHGWTVDEYLKLALQDMVTPQQLAKDTA
                     MMAEQLARSSEPDFDRNTTLTFESEELRKDGTTFLAEVTASFLWSDDGKPIGIIGITR
                     DITERKKAEEALRKSEELHIKLVNAIPDVVVRTDLAGNILFANENALRIGGYSKDEFL
                     GQNLLNFVAPEDRERVIRNLHLMLESRLGPREYHLMTRDGRKILFEINGDVLRKEDGT
                     PFAVVNVCRNITERKQIEKALREKDERLRGITENLPGVIFQFYATQNGKYGITYLSEP
                     IDEFAKILSKFDTDHLDTVFPEFLSRIHEDDRERFLSSITQAAQTASRWNFEGRVAIQ
                     SGKIIWFQGLSVPTRLPDRVIFDGILLNISERKLAEELSHQSEEKFYKIFMTTPDCIA
                     IARLHDGLIIDVNQGFEKIVGWKREQVIGTKSFEPPMNIWLDKTERKAMIADLKTGRD
                     ILDREFKFRRREGSERFGIYSARAITIDEQECVIFILQDITDRKLMDIELKRTLESLR
                     KSVGTTIQVMVSAIEMRDPYTAGHQLRVTDIARAIAAEMRLSQDKIDGLRMAGVIHDI
                     GKLSVPAEILAKPTRLTKLEYSLIQEHSRSGYEILKNVESSWPLAEIVYQHHERMDGS
                     GYPRNLKGNDILIEARILAVADVVESMASHRPYRASLGIEAALEEIEKNKGILYDEHV
                     ADVCLILFRHKGFILA"
     gene            complement(49511..50131)
                     /locus_tag="PLR13_03050"
     CDS             complement(49511..50131)
                     /locus_tag="PLR13_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18339.1"
                     /translation="MESVIFLEKISIEPPREKIYQRLGFKKRITAVSPDQQKQTDLFI
                     REAASLIVLKGMYLRLAVTKNDGKQIFLSNNVHFSSEHLSIFLRECREAVFMGATAGH
                     AVMDAIKAKTRQDDLAAAVVYDATASEMTDAALDWIMNYLNHLLMREGQTLLPRRFSA
                     GYGDFHLENQGIIYKQLHMQNAGVKINPNFILIPEKSVTAIGGICR"
     gene            complement(50165..51271)
                     /gene="leuB"
                     /locus_tag="PLR13_03055"
     CDS             complement(50165..51271)
                     /gene="leuB"
                     /locus_tag="PLR13_03055"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQC18340.1"
                     /translation="MKNNNFKIAVFAGDGIGQEIINEALKVLKIISGKKNVDFQITEG
                     LVGGTAYDRYGTPLPEESLKLALKSDAVLLGAVGGPQWENLAYSVRPERALLGLRSSL
                     GIYANLRPIVVFEELLDASPLKKDIIRGIDIIIVRELTGDVYFGKPRGIKTSKNGKRK
                     GVNTMIYSEDEIRRIAVRAFEIARTRRKSLLSVDKANILETMELWRNVVTDVSRNYPD
                     VTLRHMYVDNCALQLIRDPSQFDVIVTTNLFGDILSDEAAMLTGSIGMLPSASLGEKT
                     ALYEPIHGSAPDIAGQNIANPLAAILSVALMLRHSLSMPAEAALIENAVTETLQEGLR
                     TRDIDQPGKKIVSTSAMGDAVAGNLNKRLAGIRP"
     gene            complement(51261..51764)
                     /locus_tag="PLR13_03060"
     CDS             complement(51261..51764)
                     /locus_tag="PLR13_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQC18341.1"
                     /translation="MIFKGKVWKFGDNIDTDAIIPARYLTTSDPEVLAVHCMEDADPD
                     FMKKMKKGDMILGGENFGCGSSREHAPISIKAAGVPCVVAKSFARIFYRNAFNMGLPI
                     FESKDIVDAVKEGNEITVDSARGIITINGTDKFFKINPIPPFMEELIANGGLMQHIAG
                     KRKKNEK"
     gene            complement(51934..53193)
                     /gene="leuC"
                     /locus_tag="PLR13_03065"
     CDS             complement(51934..53193)
                     /gene="leuC"
                     /locus_tag="PLR13_03065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQC18342.1"
                     /translation="MGNTITEKILMAHTGLRKISTGDLINARVDIALGNDVTAPIAIN
                     AFKQAGGEKVFNRHKVALVLDHFTPNKDINSAQQCKAVREFASQQKITHFYEGGLVGV
                     EHALLPEQGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGELWFKVPPS
                     IKFIIFGKLQKWVSGKDLILHIIGRIGVDGALYKAMEFTGETIARLTMADRFSMANMA
                     IEAGAKNGIFAPDEVTAEYIRKKAKRHYTFYSSDPDAVYADTLEINAATIEPQVAFPH
                     LPSNVKGVSKAGRVKIDQSLIGSCTNGRIEDLRIAAKILKNRKASSNVRLIIVPATPE
                     IYRQALKEGLIEIFLKANAVISPPSCGACLGGHMGILAEGEKAVATTNRNFVGRMGHT
                     KSEVYLANPAVAAASAVLGRIASPEEL"
     gene            complement(53245..54792)
                     /locus_tag="PLR13_03070"
     CDS             complement(53245..54792)
                     /locus_tag="PLR13_03070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQC18343.1"
                     /translation="MKKKKNRVYIFDTTLRDGEQSPGYSMNTEEKLMLARQLERLGVD
                     IIEAGFPIASEGDFDAVRQIAKEIKKSQVAGLARANKLDIDRAWQAIKGAAHPRIHTF
                     ISSSDIHLKYQLKKSREQVLKEAVAAVKLARSYTDNVEFSPMDATRSDKDYLVEMVSA
                     VIDAGACTVNIPDTVGYATPEEFGNLIAYLFAHVQNIGEAIIATHCHNDLGLAVANSL
                     AAIRQGARQVECTINGIGERAGNTAMEEVVMALQTRKDLFGLHTNIKTNQIYKTSRLL
                     TQITGIPVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQSVGISDTHIVLGKH
                     SGRNAISHHLKKMGYQLNDEQINKVAARVKELADIKKDIYDEDLEVILSEEIYRGEDK
                     YSLLYLNVVSGNVAIPTATMEMQVGQDIVRKAGFGNGPVDATFAAIRNITKTNYPLMK
                     YVVNAITGGSDAQGEVTVQLKHSSYSVVGRGAHPDVIVASAKAYINALNRMEFLKNNI
                     KKVKIVE"
     gene            55438..56835
                     /locus_tag="PLR13_03075"
     CDS             55438..56835
                     /locus_tag="PLR13_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HQC18344.1"
                     /translation="MKKFFLLPLAVFFLVAVGLNSFAEEKALTLSASEKLLFNFDFIP
                     VVLTASPDGKRIAYATRVEGKQFVIVDGKAQKRYDGIGSNVVFSPDSKRSAYRAQVGN
                     KQVVVIDGQEGKRYDTVGSNIVFSPDSQRVAYAAKTGKRCFIVVDGKEGKSYEGGIGS
                     NIIFSPDSKKIAYGVLSDKKQFVVVDQQEGTHYDSIGNNIVFSPDSRRMAYQAQSDKK
                     RFLVVDGREGKRYDDVRGDFVFSPDGKQVAYVVKSAGKWQVVVDEQEGKYYDGIGDDI
                     FFSPDSSRIAYQAQSDKKRFLVVNGQEGKGYERIGSTIVFSPDSKKVAYIAQIVNGKF
                     ASVIDGQEGKPYNNIGNGSLVFSPDSKRVAYAAQSGQKRFVVVDGKEGKHYDGVGSYT
                     LVFSPDSTKIVYVAQAENKQIAVVNGKAGEKEVNKYNSIVALGKKGFAFETSDRFRFL
                     AVKEKSVYSVEVKIN"
BASE COUNT        14108 a        13178 c        14564 g        15195 t
ORIGIN      
        1 ttcccaagct ggacgtcgcc ggttcgagcc cggtcgcccg ctccaatgaa actccaattt
       61 cgaaagcgta gcgcactgaa atgggtaatt tgaattgtcc cgccaatcca cgacttgtcg
      121 ggattggcag gacaaaggga ataataaggc ggataattta taaatatcat tgataaaaac
      181 gactttaatc tgccttaata ttttacttgc tttgtccata gggatgtgct agcaaaaaca
      241 cgaaatcatc gataaagtgg tattgatgag tgggcccgtg cccacttttt tattttctga
      301 gcgattatgg actttgatgt taaagagaaa atccggcttt tagccgagcc ggttgttgct
      361 tctgaaggca tggaattgat tcacgtggaa tgcataaaga tgcatacgcg ctggattatc
      421 cgtctttttc tggataagga aggcggcatt acactggaag attgtaccaa tataagcaat
      481 cagttaggtg atatttttga cattcgcgaa gtcgtgaacg gttcttatac gctggaggtt
      541 tcgtcgcccg gattcgaccg gccgatatcg cgcgatcagg attttttgaa ataccggaat
      601 tcgcgcgtgg atgtaaaaac gggcatcaag atcgacggtg taaaaaattt tcacggcgtt
      661 ttgttggatt atatcgaaga aaacggccgg aaacttattt tggtggaagt ttcgggcaaa
      721 gtttaccgga ttccgcggca ggaaatttta aaagccaatc ttgccgatga gaagaaatag
      781 atttttacag gaattaatgc cggcaggctg agattctaat atatcagcga agaaaaaagg
      841 gaggaacttt taaatgttgc cagaacttaa acgtctgatc gagcagatgg gtaaggacag
      901 aggaataagc aaggaaatta tcgtcgaagc cctcgaagcg gcaatgctta ctgctgccag
      961 taaaaaattc ggacagaatg tggaaataga agcccattat aatgatgaga ccggagaagt
     1021 ggaggtgttc cagttcaaaa cggtcgtgga caaggttttg catccggaaa cacaaatttc
     1081 caaagaggaa gcaagggcaa cgctcgatga aggagcggaa cccggcgaca gtctaggcat
     1141 gaaaatagaa accagtcagt tcggacggat tgccgttcaa ctggccaagc agatcatcat
     1201 tcagagagtg aaagacgccg aatgcgacaa tatctatgat gaatacaagg ataggagagg
     1261 cgaactggtc aacggctttg tccagagatt tgaaggcggc agcattgtcg tcaatctcgg
     1321 gcgcgcagaa ggcattgtgc ctccttcgga acagattcat cgagaagctt acaaacgcgg
     1381 cgaacgaatc cgcgcctata tcgttgaagt aaaaaagagc cccaagggat tgcagatcat
     1441 tctttcccgc acccatcctt catttttgaa ggcgcttttc gaggtggagg ttccggagat
     1501 ttccgaaggg ttgattgata ttatcaatgt ggcgcgggaa cccggaaaaa gaggcaagat
     1561 cgcggttaaa tccaaagaca aggatattga tcccgtcggt gcctgcgtgg ggatgcgggg
     1621 gtcccgcgtg caaagcgtag tacaggagtt gcgcggcgag aaaatcgata tcattcccta
     1681 tagcgaagat ccggccaaat atgtcagcag tgctctttct cccgctaaag tcaaccgtat
     1741 ttttattgat gatgaaaacc ggaccatgac tgtgattgtt ccggatgatc agctttctct
     1801 ggctattggc aagaacggcc agaatgtccg gctggcggtc aaactgaccg gatggaatat
     1861 tgacatgaag agcgaaacaa tggagcaggc tcaggaggaa ggaaaaagcg gacagaatga
     1921 tctgaccaag attaccggca tcggtccggc catggcggaa aaactgttca gcaaaggcat
     1981 aaagagtatc gccatggtgg cggcgacgga accggaattg ttgtctcaga ttccggggat
     2041 aggcgaaaag acggcccagc agtggattga aacggccggt aaaattttaa acgaggagat
     2101 cgctggttcc aagagtgatt catcatctga aaattaagaa atttttcatg gagattgttg
     2161 cgcatgccta aaaagcgggt ttatgaattg gccaaagagc ttggaatgga gagcaaggtt
     2221 ctgatcgctc gtttggaaaa aatcggcatt tcggttaaat cggcgtcggc ttctctggaa
     2281 gaggctgatg cggagcgggc caagaaagag ctgaccgccg gagaacttcg tgaagtcgtg
     2341 gaacagagga tcaagaccac ggttatcaga cgccgcacgg tcgtgaccgc tgtggagaaa
     2401 ccgcctgaag aagtccccct tgaggaagcc aaacctccgg aaaaagaacc tgccgaagac
     2461 aagcttaaaa aagcgcctcc gggaaaaact gaaaaagtta aaaaggaaaa gcctggggaa
     2521 gagcctgctg ttcctgcaga gacggtttcg gaaaaagcgg tgaaggaggt cgttcctccg
     2581 gcagcggttg gaccggctgc cgccgaaacg aaggcttctt tggaacagaa agccgcgccc
     2641 cttgaaaagt cggcagagga aaaagctgtc aaaccggaaa caaaggaagc gccggttgct
     2701 cctgtcaagc cgacgatcat taccaaacat aaaattatca ggccggaaac aggaaaggcg
     2761 gttcccccca aagtgccggc tgccaaaccg gcggaaaaaa tatcacaacc gtctgcggaa
     2821 aagccgaaga aaaaaggcaa atatcaggtc gaggtcttga tcgaagaaga gaagaagatc
     2881 ccgaccaaga aaatcctgga aaagaagatc gaaaagcggc tcaaacggca tgaagaggat
     2941 caggaagttg tttttaccaa atggcgtgag gggaaaaagg ccgcgccgac caagatgaag
     3001 aaaacggaga ttaccgttcc caaggccatt aaacgacgga tcaaaatcgg cgaaacgatt
     3061 accgttggcg aactggccaa acgcatgggc atcaaggtca gcgagattat caataagctg
     3121 atgtccatgg gcgtgatggc gaccatcaat caggccatcg attacgatac cgccagcatt
     3181 gttgcgggtg aattcagttt ccagctggaa tccgcggacg tggaatttga cgaatccgtt
     3241 ctgaaggctc cctccacacc ggagaatctc aaaccgcgtg ctccggtggt gaccatcatg
     3301 ggacatgtcg atcacggcaa aacctccctg ctggatgcca tccggcagac caatgtttcc
     3361 gacggtgaaa cgggaggcat cacgcaggcg atcggcgcct atcatgtgca tgtcaacggc
     3421 cgggatattg tgttcctgga tacccccggt cacgaggcgt ttacagcgat gcgggcccgc
     3481 ggagcgcagg ttaccgatat cgttgttctg atcgtggccg ccgatgacgg cgtcatgaat
     3541 cagaccgtcg aggccatcaa tcatgccagg gttgccggcg ttccgattat cgtggcgatt
     3601 aataaaatcg acaagccggg tgcggatcct gaaaaaataa aacaagcgtt gaccgaacat
     3661 aatttgcttt ccgaacagtg gggcggggat acgattttct gtgaagtatc cgccaagaaa
     3721 aaaatcaaca ttgaagaact gctggaaatg attctcctgc aggccgatgt catggagctg
     3781 aaggccgatc ccgatcgccc tgttcgcggc attatcatag aatccaaact ggataaggga
     3841 cgcggtccgg ttgcgaccgt cctgattcag gaaggcacgc tcagggaaag cgattcgttt
     3901 gtttccaaga ccgaattcgg ccgtgttcgc gcgctgatta atgatcaggg acggcgaatc
     3961 aaggaagccg gaccgtccat gccggtggaa gtgatcggat tttccagtgt gccgcagacg
     4021 ggagcggaat ttttctgcgt cgacgatgag aagaaagcgc gtgcgattgc cgattactgg
     4081 accagaaagg cccgggaaaa ggaactttcg tctctgtcca agatcaccct ggaacagctt
     4141 tatcagagga taaaagaagg ggcgaaggaa ttcaacgtta ttgtcaaggc cgatgtgcag
     4201 gggtcgatag aagcgatttc cgacgcattg aataaacttt caacggacga tatcaagctg
     4261 aagattattc atagttccac cggggccatc agcgaaacgg acgtgatgct tgcgtcggct
     4321 tcccaggcta ttattatcgg ctttaatgtg cgacccgatg cgcgggtggt ggatgttgcc
     4381 cagcgggaag gagtggaaat caagctctac gatatcattt ataatgtgat tgcggatgtg
     4441 cgggcggcca tggaaggtct tctggaacct gaataccggg aggttgttca gggcagagcg
     4501 gaagtgcgcg aacttttcaa ggtgccgaag gtcggtacca ttgccgggtg ttatgtcacg
     4561 gatggaaaaa tcatgcgcag caataatctc aagcttgtcc gtgacagcgt ggtcgttttt
     4621 gacggaaaga ttctgtcatt gaaacgtttt aaggatgacg cccgggaagt gctggccggt
     4681 ttcgaatgcg gtatcggcat cgaaggattt aatgatattc atccgggaga tgtcattgag
     4741 gcgtatgcga tagaaacgtt ggaaaagaaa ctggaaccga atgctgataa gcgatagttt
     4801 ttttatatcc gaacggttct aaaaaaatcg gggctcccga atgcttcagg tttccggaga
     4861 atgcttatgg ttgtcggata cggcatcatt aatctcagaa ttccggaaag cggctcttta
     4921 aaaagcaagc gaagcgtttt gaaccgaatt ttaaaacgcg cacaaaatga atttaatgtt
     4981 tccattgcgg aaacaggcca tcttgataat tataaactgg ccgaaatttc ttttgcggtt
     5041 gtcggcaacg attcccgtta tatcaacggg aaggcggatc atctgctgcg ttttatcgat
     5101 catctgcaga ttgccgagat tgtaaactgc aaaatggaaa ttatggttgt ttcgagtttt
     5161 ctggaaacag ccgacgggga tatacagaag tacgatgaat tttaaaagag cggaccgggt
     5221 cgccgaatta atccgggcgg atatgtccga aataatcatg aaggacgtga aagacccgcg
     5281 tctgcattcg gtaaccatta cggcggtcaa ggtgtcggat gatttgcgca atgccaagat
     5341 ttatttcgtg gaaatgggca aagacgaatg ctctgttgaa attaaagcgg gactggctca
     5401 ggcaagcgga ttcatacgcc gggagctggg caagaaattg cagttgcgtt ttgtgccgga
     5461 actgatgttt attcatgata cgtctttcgg ttacgggaac cgtatcgaaa aattgttggc
     5521 acagatccac agtcaggaaa atgaaaatga ttcatgatat tatccaagcg attcacgaag
     5581 gacagaactt tctgattacc gcccatgtgc ggctggacgg agatgcgctc ggttcggaac
     5641 ttgccgttta tctgatgctg aaagaactgg gcaagaaggc cgttgtctat aacaaggatc
     5701 gcacgccgga acgttatcag tttctgccgg ccacgcacaa catttctcat acgttaacga
     5761 atatcgagca gtacgatacc tgcatcgtgc tggattgcag cgatttgtcg agggtcggcg
     5821 atgaagcgga aaatatcaga aaaatcaaga cgctcattaa catcgatcac catgtttcca
     5881 ataacggatt ttgtgcttta aaaatgctgg atgccaaagc cagttccacc ggcgaactcg
     5941 ttttccgcct gatgcaggac atgcgcttca aaatgtccag agacatctgc accaatctgt
     6001 atgcggccat cattaccgat accggcagtt tccgctattc gagcacgaca aaggagacgt
     6061 tcatggcggc gggggtgctg gtgggggaag gggcgagtcc ccagcgcatt gcggaaaata
     6121 tttatgaaag cgattctccg gcccggctca aactgatggc ccgggcactg tcgacgttat
     6181 cgcttgatct ggaaagcaag gtcgggtcca tggtcgttac ccagaaagat ttgctggaaa
     6241 cgggcgccgc ctgggagcac acggaaggat ttgttgatat accccgcacg gtgaagggta
     6301 tcgaagtatc ggttctctat acccagagag gggaaaaaaa tttcaagctg agtttgcgtt
     6361 ccaaagccaa ggtcgacgtg gaaaaagtgg ccaaacggtt cggcggcggc ggccatattc
     6421 atgcctcgtc ctgctggatg gacggcgata ttgaaaccat caaatcgcaa attattcagg
     6481 cggtcaggga attgtagtgt taatgatgaa cggcgttatt gtgatcgata aaccggcggg
     6541 gaaaacctcg cacgatgttg ttctggatgt gagaagagct ctggggataa aaaaagccgg
     6601 ccatacagga acgctggatc ccctggctac gggcgtgctg cctgtttgcg tgaatgaagc
     6661 caccaaactg gccggttttc tggcgggcga caaaaaagaa taccgggcca cgatgttgct
     6721 gggtgtgaca acggacaccc tggatattga gggaaacatt acgggacaat ccgataaggt
     6781 tgtgtctgag gaagaaattc gatcggtcat gatgcggatg atcggaacca taaaccaggt
     6841 cccgccggcg tattccgcgg ttaaacattg cggcaagccc ctgtataaat gggcaagaaa
     6901 aggggttttc ccggatttga agccgaggga aatcaaaatt ttcagcctgc ttgtggagaa
     6961 tatttctttt ccgtcggtga agttccgtgt ggcctgttca aaagggacgt atatccggtc
     7021 tctttgtgcg gatatcgggg aagcgctggg aacaggggcc tgtctgagcg ggctgcgccg
     7081 tttgcaaagc gggatttttt cagaggcgat ggccgttaat ctaaatgatt ataagttccc
     7141 ggaagcaaaa gagaaactgg ctgaaaaaat attgccgatg gctgagcttc tgccgcttct
     7201 ggccaccatt gaactggaag atcaatgcgc ggtaaaactg cgcaacggct ggcagccttc
     7261 ggtggaaatg ctcaaggagc acgatcttcc cttgcttaaa gcaggagata tgataaaatt
     7321 cgtcagtcgc ggcaatcttc tggctgttgc ggaaatgctg gccccggtca gcgagctggc
     7381 tgcttttgac ggaaaaaggc aggcggccag aattctccgg gtgtttaatc agccgtgaat
     7441 gaagaacata aaaaccaaca taaaagatta taaattgtaa aaaggaggaa aacgcgtgtt
     7501 aagttcggaa aagaggaaag aagtcattga taattatcgg ctgcatgaaa aggatacggg
     7561 gtctcccgag gtccagatcg ctcttttgag cgccagaatc gagtatttga cggagcattt
     7621 caaggcgcat aagaaagatc atcattcgcg ccgaggtctg ctgaagctgg tcggccagag
     7681 aagaaggctt ctcaattata tcaaagaaaa tgacatcgag agatacagaa ccgttattaa
     7741 acgtttgaat ctcagaaagt aaaagaatta ttcaagggcg gaaggcataa ggcaagctga
     7801 ccgtaagcga atcgatcgtt gaacggttgt tgctttatgc gtttcgccct tgaatttgaa
     7861 aagaccagca ctcacaaaca attatggagg aaaatgaatg agtaatgtat tcagtgcgga
     7921 tttcgccggg cggaacattt ccctgaaagc gaattatgtg gccgcgcagg ccgatggggc
     7981 cgttttggtt tactacggcg acacagttgt attggttaca gcggtttctc tgaaaagcac
     8041 gcgggaaggc gtggattttc tgccgctgac ggttgattat caggaaatga cttttgccgc
     8101 aggaaaaatt cccggcggct tttttaaacg ggaaggacgt cccaacgaaa gggaaattct
     8161 gacatcgcgc gttattgatc gcgcgattcg tccattattc cccaagggat atttttctga
     8221 aacacagttg gtggccacgc ttttatccgt ggacaaagaa aacgatcccg atgttgcgtc
     8281 cctgatcggc gcttccgccg ctctggggat atctgacatt cctttcaagg ggccgatcgc
     8341 cggcgtgcgt gtcggccgga taaacggcga atttgtcgcc aatatgtgcc cggagaagat
     8401 gaaggaaagc gagatgagcc tttttctggt gggacgaaaa gtgacacccg gaacgtccgg
     8461 ccgtccctat gacgtggaac tggtcatgat ggaaggtgaa gccaaagagg ttccggaaga
     8521 tatcatcgtc gatgctatta aattcgggct ggaatcgatc cggccggtta ttgacctgca
     8581 ggatcagatt caggcatcta tcggcaaggt taaaaggccg gttgaggagc cggttccgga
     8641 tgaagagctt gttgctcgcg tgcgtgaaga agccctgccc ggcttgaaag aggggtatgc
     8701 catgccgcgc aagctggaac gttacagcaa actgggcgac gttcgcgccg aggttataaa
     8761 aaatatcggc ggagacgacg ccgtcctgag taaaaaagtt gccggcatta tcgagaacct
     8821 ggaatcccgc atcctgcgcg atatgatcat tcaggacaag aaaagaattg atggccgctc
     8881 ttccgttgat attcgtccga tttcttccga ggccggtgtg ctgccgcgcg cccacggttc
     8941 cgcactgttc agccgggggg aaacacaggc gctggcggcg ctgacgctgg ggacatccgc
     9001 cgatgagcag cggatggatt acgtcggggg agaagagatc aggacatttt tattgcacta
     9061 caactttcct cccttcagcg tgggggaggc caagatgctg cgcagtcccg gacggaggga
     9121 aataggacac ggagcgctgg cgcgcaaagc tctggtgccg gttctgccgt ctccggagga
     9181 atttccctac accatccgga ttgtttcgga aattttgtcg tccaacggtt cgtcatctat
     9241 ggccacagtc tgcggcggta ttttatgcct gatggacggt ggggtgcccg tgaaagatat
     9301 cgtggcgggc attgccatgg ggctcctcaa ggaaggcaat gagatcgtta ttctgtccga
     9361 cattctgggc gatgaagatc atgccggtga tatggacttc aaggtatgcg gtacggaaaa
     9421 gggcgtaacc gccatgcaga tggacatcaa gattgacggc ttgaccgagg atattctgcg
     9481 caaggcgctg gctcaggcca gagagggacg catgcacatc atcggtaaga ttcgtgagac
     9541 gctggctgcg ccgagaggcg acatttctca atatgcgccc cgtatcacga cggtgaaggt
     9601 caaggaagat caggtgcgca ccgttattgg ttccggcggc aagaacatcc gtcagattat
     9661 cagtgagacc ggtgtgacga ttgatgtgga agatgacgga accgtcacca ttgcctccgc
     9721 cgatgccgag gcagccgccc gtgcggtggc catggtgaaa tggctgacgg aagaagccga
     9781 gattggaaaa atttaccgcg gcaccgtcaa gaagattgtc gatttcggcg cgtttgtgga
     9841 aattctgccc ggaacggaag gcctgttgca tatttcacag atcgccaagg aaagaatcgc
     9901 caaggtaacc gatgttttac aggaaggcga tgaagtgatg gtcaaggtgc tggaaatcga
     9961 caagtccggc aagatccgcc tcagccgcaa agaagcactg ggcgcggaag tnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgacaagt ccggcaagat ccgcctcagc
    10141 cgcaaagaag cactgggcgc ggaagtcaag taatacattc ccggttaagg gttatggaaa
    10201 aacaagccgt tccgcatgca acgatacgtt ggtgcggaac ggatctcttt atcaaccatt
    10261 gtaagcagtt ctcccaataa ttatttctgg tcgaaacatg aaaaaagtca aggtttgtat
    10321 tcagagactt ccgggaaacg atgatatccc cctgccgcag tacatgaccg atcaagccgc
    10381 cggcatggat attttttcgg cggtggctga agaagaaatt attttgccgg gccaaagaaa
    10441 aaaaatcgca acaggcatcg ccgtcgctct gcccgatggc tatgaagcgc agatcaggcc
    10501 gcgcagcgga ctggcgctga aaaacggcgt taccctgtta aatacaccgg ggacgattga
    10561 cgccgattac cggggagaaa tcggtctgat tgtgattaat catggggaag agccctttat
    10621 cgtcacacga ggcatgcgtc tggcacagat ggtggtgcag cgcgtctgcc gggtggattg
    10681 ggaggaaacc gaaacactgg caaaaacagt ccggggcagc ggcgggttcg gccatacctg
    10741 aacgcttctt agcttttgca attggaaaca aagagtggta tgaaaatgga caatgacgat
    10801 gtgacgataa cgatacggta tgaaaaaatt caaagttaag aaaacaataa aagaaattaa
    10861 cgaaaagatc aaagaaggac gtgctgtcgt ggtgacagca gaggaaatga tcgacgttgt
    10921 cgaacgccac ggcgatgtgg aggccgcccg caagatagat gttgtgacca ccggaacgtt
    10981 cggaccgatg tgctcctcgg gggcgtttct caatttcgga catacgtctc ccaagatcag
    11041 ggcttcccgg gtgtggttga atgacgtcgc cgcttacggc ggtattgccg ccgtggattg
    11101 ttatatcggc gcgacggaag tggccgaaaa cgatccgctc aacagtgttt atcccggtga
    11161 atttaattac ggcggcggtc acgtcatcga ggacctggtt tccggaaatt ttgtcaagct
    11221 tcgtgccgaa gggtacggaa ccgattgcta tcccgcccgg aaattcgaaa gaaacattac
    11281 gataaaagat ttgagggatg cgattctgtt caatccccgc aatgcgtatc agaattacaa
    11341 ttgtgccgtg aatctgtccg acaaaacgat ctacacttac atggggatgc tgcgtccgca
    11401 aatggccaat gcggcatatt ccacgtccgg acaattaagt ccattattta atgatcctta
    11461 ttacagaacc atcgggatcg gtacgcgcat atttttgggt ggcgcacagg gatttgtcgc
    11521 ctggccgggc acgcagcata atccgaaggt gctgcgcgga tccaacggtg ttccccggga
    11581 gggagccggg acgattgccg tgatcggtaa tcttaaagaa atgtctgcgg attggctcac
    11641 gggtgccagt atcttgggtt atggggtttc tctttttgtg ggcattggta ttcccattcc
    11701 tgtgttggat gaagagatgg cccgttacac ggccgtcaga gacgaggata tctataccca
    11761 ggtatacgat tacagcatgg attaccccaa gggcagtcca aaacctctgg cagaggtcag
    11821 ttacaaggat ctgcgcagcg gcaccattgt tttggaagga aaaaaagtga ttacggcgcc
    11881 cctttccagt tacttcaaag ccagacagat cgcctatacg ttaaaggaat ggattgagtg
    11941 cggccggttt attctgggcg aaccgcaaca aaatctgccg tcgttgtaga atcacgagag
    12001 tgccggcgac gcgaaaacct tttcgcacgg gcatctcaaa tcgttggatc gagcagtgta
    12061 ccggcatttt ccggtaaaac tcaggcacaa aatttaatca aaaaattttc gagaagcagt
    12121 tgcgggttgg aggcggatgt tttcattgaa cgctcgaggc tgaggagttc atccagcagg
    12181 tcggtcaggc gggaggttgt aaagcggacg caattacgca gtgcgttaaa aacggaaaag
    12241 ggatgctggt ttaaaatcag gatatcgttt tttctgccgg tgggattcaa cgcgctgaag
    12301 accggataaa cagtgttctg aaaccagccg tattccatgg attggttgaa tttcggcagt
    12361 ttcccggaat cgaccatgac tctggcctgc agcaggagcc ggatttccct ggatagcatg
    12421 gtcatgatca ttaattgatg gatgccctga tccagaagat gatttaaggt gctgattgcg
    12481 gccagttgat ttttctcgct cagagcattg gtcagagcga aaatttcctc ctcccttgtt
    12541 ctgcccaccg cttcttccac atcctcttta tcgatgagcg cccgttctcc gacgaaggaa
    12601 ataagcttct caagttccga cagagagtta cggaaatcga aaccggtttt tttgcccagg
    12661 gcaatccatg ccgccggagc catttttttc ccgcagccat ccaatagttt ttgcgcttct
    12721 ttctgaagga tttctttgcg tgcgtactcc ttcttgaccg ggctgaaata gttgacgacg
    12781 ccgatatttg cgacgatttt atagagtgat ttccttttat ccacggcttc tgcggtgaaa
    12841 atcaggcagt tgcccgcggg caatccggct ttcagaacct cttccagttt ttccgtttta
    12901 ctggtggaag cgtccgtcag tccggccgtc tggcatatat ccagaattcg gggcagccac
    12961 ttttcccgct cgtcgccggc atctcccttg acaaggccgc tccactgttc atccgtaatt
    13021 ttctgccagc ccgtaccata aagatcctcc agggaaaatc ctgcaatatt gaggaaggtt
    13081 agaaaatatc tggccgcttt ggtcgggttt tcgttgatgg tggtacgtat tttggctgcg
    13141 agtttggcca gatcatcacg cgatgtgaaa attgtggtgt cttgcacgac gatgaccttt
    13201 gtgtcgccca gaagagaagg ggtcaggaga tgatccatca ggctgtttat ctctgtgttc
    13261 tcgccatcca gataaaacag actgaaatcc cgatcggcct ccggaacaag aacggcaatc
    13321 attttgttga gggcttcttt gatgagatat tcttcttcac cgtaaagaag ataacagggt
    13381 gcaacaatgc cctttttcag attggttaag gttttttcca tgacgcatca ggggctcaag
    13441 gacgattata cgataacgta ttttttaatg agtttgacaa catcttccgg ttcatcaacg
    13501 atctgaatta attccatatc aacgggaaga attttgtctt ccgccagcat cgcattgttc
    13561 agccagtcga tcaggccttt ccagtattcg cttcccatga gaatcagagg gaaagacttg
    13621 attttttttg tctggatgag cgtcagggcc tcgaaaagct catccatggt gccgaagccg
    13681 cccggcataa tgacgtaggc aaccgcgtat ttgacgaaca tgactttgcg gacaaagaaa
    13741 tatttgtaat caatgtggat gttcgcgtaa tcgtttggtt tttgttcaaa gggcagctta
    13801 atgttcaatc ccacggattt ccccccggct tctgcggcgc ctttgtttgc cgcttccatg
    13861 attcccggtc cgccgccggt gatgacgttg aatctttccc caaccaggag ttttgccagc
    13921 ttttcggttt tctggtagta gatgtcgtcg ggtttgactc tggcagaacc gaaaatggtg
    13981 accgcattgt cgacattgga gagcgtttca atcccctcga cgaattccgc catgatacgg
    14041 aaaacccgcc atgcttcgtt gacggacaga gcgtcaatga catattgctt ttcatccata
    14101 gatcgacacc ttcccttcga gcggtaatgg tatggaatag gaataaaaaa atgagtttaa
    14161 taacgacggc gtctctgaac cttggccagg cggcgctgag cttctatggt ttttcttttt
    14221 tgttttacag acggcttttc atacgcccgg cgaactttca gttccttgaa caagccgctt
    14281 ttcgaaagtt tgttcttcaa gattttcagt gctttttcca cgtcattgtc gattactttt
    14341 acttccaaat ttattcctcc ctgttaatag ttgttagtga tcgtgtgact tctgtcaacg
    14401 cttattcatc ttgtccgata cgtcccgggt tggcggattg tgtccggttg cctgcgccga
    14461 tcggataatt tgacttacaa atttcattgc gcttcgctgt gcttgttctt ggcgagtgtc
    14521 acatcccgtt tcgttccgcc cctccggagg cattcgatca gcaagtgtcg gtgcacttcg
    14581 tttctgaaaa ttggtctcaa attccacgtc gcgacgatcc tgggataatt cgactaacac
    14641 cacaaacggc actgcgttta ttttcactga gtttcataac aaaaaaaggg cagtacggca
    14701 aagcgaaaaa gctgccgtaa tccaccctgc aatgtcccac taattttaaa tcacgctcct
    14761 tattctggag taatgttttt taaggcgtcg agaaaggctt gattttcctt tttactgcca
    14821 accgttactc tgatgccgtc aggcatccgc ggcgcaagat tcagcgtctt gacgattatg
    14881 ccttgcgaaa cgaggtcttt atatatgcga ttggcatcaa aagcgcaact aaaaaaaata
    14941 aaatttgccc gggaagggta tgggacaatc catttgattt tttttatttc ccggtagagt
    15001 tcctggcgtc tctgaattat ctctttgatt tgtttgatga attctccttc atgatcgaga
    15061 aaaaatcccg cggctttctg agacagagca ttgacattat agggtaatct taccttgtcc
    15121 aactgatcga taaggtcggg attcccgatt aaaaagccca gacgtatgga agccatgccc
    15181 aatttggaaa gcgtctttag aaaaacaaca tttttatatt ttttcaacag cggaagcagg
    15241 ctttgtccgg caaaagcgct ataggcttcg tcaatgacga caacgccggg ggcttttctg
    15301 ataacggctt ctattctttc gcggctgaaa aggtttccgg tggggctgtt gggtgaactc
    15361 aggaaaatca acgcgggaaa attatttttt attctggcgg cgatggcatc cacatccaga
    15421 tcgaactttt gatccagagg aacgtccacc actttattac cggtattgac cgcgatgatt
    15481 ttgtacatgg aaaatgttgg agaaggaacc agcacgcctt tgattttgcc cctgaaggcc
    15541 atgcagagaa tctggatcag ttcgtcggag ccgttgcccg gcatgatcat attttttttt
    15601 acgccgtaat agtcggcaaa cctttcgcgt aaatccggag cgccggcggc agggtaacgg
    15661 ttgaaatcaa tcgaacgcag cgtgtctgcc agtctccttt ttaacggttc ctggagggta
    15721 aaaggatttt cattagcatc cagtttgatc gcatcggtga tttccggcgc cgaataaccg
    15781 ttttgtgtca atattgcttt tgctataaga ttccgaatga tcatgttgaa tcaaaccgcc
    15841 ttaaagtatt tttcccgctc gatattccac aagccatctg tatatttttt catcaattcg
    15901 tttttgagtt tttcctccgc gggcgtcaga gatccttttc ccagatgtat tcccagttcg
    15961 tcgcagaaac ttttttttaa tgtttcgcat atttcttccg ctgtaagcgg ttgacacaga
    16021 tattcgttta tcgacgtcac ggattgtttt aatttttcga tttgctccgg ggaacgggag
    16081 gaaagcagga gattcgccgt cccctccgga tcaaacctgt aaaaaagaag cccgtgctgt
    16141 aaaaatcctc cccgtcttct gacctgcgcg ctgccgcaga tcttgcggcc gttcaccaga
    16201 atttcattct ctgccgatgt tgaaaaacag caggcaactt tttctccctg gttcctttca
    16261 tcgccggttt cagccaagcg ggcatcaatg cccagattgc caagcccgcg gacgagacat
    16321 ttgctgatga ccctgtatgt tcctgatatg ttgtcgggaa aaattctttc tccgcttccc
    16381 gccgttacgg agtatgtgat ttcattgcag tgaaaaacgg cccgcccgcc tgttattctc
    16441 cgaacaacat caatcccatc gtggcggcat tgcccgaggt ttaaatcctt tttcgcatcc
    16501 tgaaaataac cgatggaaac agcttccggc tctgatccgt aaaatcttat cgtggattct
    16561 gttttactgg ttatgactcc gcgaaaaatt gcttcatcga tggccatgtt ctcaaaaatg
    16621 ttattcgttt ggtaattgag aaatctccag cccataaaga agaattattc gctttcttcc
    16681 tgcgctataa aatcatggta attggacgct gccagaaggt cttccagttg ttcgagattg
    16741 tccatttcaa tcacgaccag ccatcctgtg tcataaggat ctctgttaat gatactgaca
    16801 tcttcaagaa tatcttcatt gacaacgacg acggtgccgc tgacaggtga aattaaatcc
    16861 accacttcct tcacggactc aatcgagccg aatgtttcat cacgctccac ataggtatct
    16921 gtttcgataa actcgacgga aagaatatcg cccaatcttt cctgtgcgta atcggtgatg
    16981 cctatggtgg ccatatttcc gtcaaccttc acccagatgt gttcgcaact gtaaattata
    17041 tcttccggaa aatccttcat gatagaacaa cctctttctt tattctataa tggtaagcct
    17101 tttatccatt tttgtcagct tttcgcaact gttttctttc agtaacacgg tatcttcaat
    17161 tctgactccc catcgtcccg gaagatataa tccgggctcc acggtaaata ccatccgcgg
    17221 ttttaaaata tcgtctgaaa gggaagacag gcgcggtgcc tcgtgaactt ccagaccgac
    17281 accatggccc gtgccgtgcg taaaataccg gacatacttt ttcccgaaga cactgcggac
    17341 gtgttgatcg attgccgccc cgggaatgtc tgccctgagt aattccaaag cgcgatcatg
    17401 agcgtctttc accacctgat aagcattttt ttgtctgtct gtcaatttcc caaaagcaag
    17461 ggtgcaggtt tcatccgaac aatagccctg atatctcacg ccgaaatcaa tgacgacaaa
    17521 atcccccttc ctgatttttc ttttgcccgg ttttgcgtgg ggcagggaag aattttcacc
    17581 ggaggcaaca atggtttcaa aggcgatgcc gtcggcgccg ttttcccggg cggcgatttc
    17641 cagttgccag gccgttgcct tttccgtcca gccgggcgtg atcctgttga cgaccgattg
    17701 gatggcggct gaagaaatct ccgccgcttt tttcatgatc attatttcat catcgtcttt
    17761 gatcgcccgg agtgttttta attcatcggt ccagggtatg aattttgttt caggagcctg
    17821 agcggttaat ttattatgga ggctccaggt gacaaaatca gcttcaaaac cgatgtgttt
    17881 taatttcagt tgcctggtca taagcgcaac tgcttcgatt ctatcagcgc attctatgat
    17941 tttgattcct tccgtttcca ttcccgcctg agtggtgtaa cggccatcga caaacaaaat
    18001 gggcttttta gggccgacga agagaagccc ttccgagccg gtgaaaccgc acagatagcg
    18061 gacagtgctc atattgcaaa tgatcagacc gtcgatttta caaacggaca ttttttccag
    18121 aattccggaa agtctttgcg gcattttttt attattaagc ggcattcatt ttcaatcttt
    18181 caatattttt cagccaggaa gataaaatct tgatcatctc gtcgtttttc tttgtgttgc
    18241 cgtttacggt tcccgatgca gccggggcgg atatgaggtt gttgagcatg gacagggcct
    18301 gccggttatc ggggtctttt ttgagaattt cgtctaaaat ggtttttgcg ttctgaagat
    18361 gtccctgatc aatgtataac tgagccaggg taactgtaaa aaaatcctgt gcaggaatat
    18421 ttcttttacc cttttccggg acggtgggat ggtcttcctg atccaacaag gtcatttttt
    18481 caatgatttc agacgctttt tccgtttccg gccgcaaaga gatgaacctt tcgtagcagg
    18541 cggcagcttc ctgatgaaaa cctttttcac ggcaggcgtc gccggaacgc tcacaaacaa
    18601 gatttaacgc ggaaatacat tcttccgttt gaagaagtat gttacgcatg tcttccagtc
    18661 ttcccatgtt aataagcgct tcgcagcaga tgcccagggc atcggcatct gcgggacagt
    18721 ccagcaagtg attttcggcg ataaggagcg cttccccgaa atttccttcc cgcaaaaatg
    18781 cttctgcttt gcgtaaaagg agattttgtt ttttgtcaaa tctgctattt tccatgttta
    18841 atcaaataat taaatcaatg tgcaatttgg cgttacgtaa cacagcacat ggggagtgtc
    18901 aagaatggaa aagacaggat gttttacaca gattactgta atgattgcag gtaatttctg
    18961 gctttcaatc cgaaagaaga atcgggggac aacttgacga cattttcgaa tgcccggcgt
    19021 gcgtttttgc tgtctttgtt tctcaggtag ctctggccta aaataaagta ggcatccaca
    19081 agcgatggat ccagctcggt cgattttttt aaatgcctga tggcttccat gtcattgttt
    19141 tgttcataat ggataaggcc gatatttttt tctacctgcg gacgcaatcc cgtcatggga
    19201 tctgttcgga gcacatcctg atatttatcc agagcggttt tgtaatcttt ttttgaataa
    19261 taggcccagg ccatattata cagagccatg gacggcgtat catacaaatg attgccgagg
    19321 gctttttcga attctctgat ggctttgtca tagagctgta tttccgaata tagcgtgccc
    19381 aggtagttgt gagcttcgga ataatgcttg tccaaagaga ccgccttttc aaattcctcg
    19441 atggcctgtt ctctcatgcc tttgccgtga taggcaattc ccagataata atgcaccttg
    19501 ggatttctgg aatagtattt ttcagcttcc aggagttctt tcagagcgct gttgaattgt
    19561 ccgatctgca ggaaagaaat gcctttgttc aagaagatct cggactgttc tctgtgccag
    19621 ggtgtatatg tacaggaaaa acacagcagg attgctgaga acacaaatat taatacagct
    19681 ttgttcgcgg ttagtttcat tttaatataa accagtctgt tacaaatccg atactggatt
    19741 gcagatcaga gtcaaataaa agccgataat ctgatggccg gcagttggga tttttcaggt
    19801 tccttctgct gtaaagtcgt tattcaatat ttttggagtc acaaaaatca gcaattgccg
    19861 tttatctctt tttgtgctgt ccgttttaaa taaccagccg agaacgggaa ttttttgcag
    19921 ccacggccag ccacttacgg ctttgctttc cgttgatctc aaaatgccgc cgacgacgac
    19981 ggtgtctcca tcgttaataa ccaccttgga ctccacctcg cttttgacaa ttggaggatt
    20041 gccctgggga ttcagggctg ctttcgacca gtccggagta tcattgctgg ccttgatatc
    20101 catggagatt ttcccttcgt cggttaactt cggcgtcact tccagtttta ataatgcttc
    20161 cttaaaggaa acagttgccg gcgatgttcc cgatgccgga gtgatataag gcacttcatc
    20221 gccctgttta ataatggctt tgataccttc catggtcacc accttgggcg ccgaaatgat
    20281 ttttccggtg gaattcgttt ccagtgcggc aagttgcgtt tcaagaaaac cactggcgcc
    20341 cccaaagatc agaccgattg ccggcgaagc cagggatgtg ggcaaattga ctgcggccat
    20401 cgctaacgga ttcccatcgg catccctgat gccgatttga gaaggatatg taagcgcctg
    20461 ctggtttccg gtggcaacat tgctgcctcc ggatgcgccc aatccatact gactgctgcc
    20521 gatgtgttgt ctctgtaccg taccaaatcc ccattggaca ccgaggttcc tgacgaattc
    20581 ttcggttgcc tcgacaattt ttgcttcaat caagacctgc ttcaataatc ctttttcggc
    20641 catcattttc tgttctctgg cccggacctc ttctctggcg gtttcttctc gcttgctgcg
    20701 ttccatgctg gttacgataa taacgttgtc ttcctgtttt tttgtcagac cgttaacttc
    20761 aaggatggtt tccagggctt ttgtccaggg aacatttttc atggaaagcg taatgttgcc
    20821 tttgacttct tcgctggtca cgatgcttac attgccgtat tcagccatca ggcgtaaaac
    20881 gcttttgatg tccgcatcct gaaaatcaag agagacgatt ctatccatat attttttcgc
    20941 cggagatttt tttaccgaca cgtccaggtc ttctttttta aaatcgtcgg ttttttccgg
    21001 ttctgcgctg actttggcca ggggcttttt agaagcagag gcttttgata tcgttctggc
    21061 ctttctttcc tcaatgccgg aaacattaaa atcaattaaa acattttccc cttcctgtct
    21121 tattgaatag ggaacggcct catgcggata aatcgtcaga taaacccagt ttttcccctg
    21181 gagctgctgc cgcgaaggaa cgatactgat aatattgttg agttcccctt ctcccagcgg
    21241 gcggcagaga ctttccggga tatcggtatc ttcaagtctg atcaggtagc ttccgttgtc
    21301 tttgctctta acatcaatga gcggttgctt ggaaacaacc agatgaatcc tttcttttcc
    21361 gggcaacttg gccagcatga tacttttcaa catggtgatg ccggcagtgt tttcttcgga
    21421 agcattgaaa gctgcaaaag aatttcccat gatacatgtg aagatccatg tgataattac
    21481 aaaagccgcg aatattttag ggaaatattt tttcatgact ttacctcatt ttaatttttg
    21541 tgcagtttta aaattacttt gtttccttta ttattttcat attcttctac aatgaccctg
    21601 tcaggcagaa tgtcaacgac tttgccgtta tgcaatccga ttcttgttcc gaccagcaga
    21661 ggataaaact ttttagaagc gtcttcagcg atcgctaccc gatgatcctc attgccgaca
    21721 atgccgacga ctttataact tctggcttcc gctctttgca aagggaaaat ggacggtttg
    21781 atttttttct cttccggctt cgtttttttg acggtttcaa tgacaataaa aggtcggaac
    21841 ggatccggct tacccgcggg attgtaactg tatgtgtccg cctgattcgg cacggttggt
    21901 gccggcatag gggtttggac agttggcgtt gcagtcgcat ttgccgcgga agaaaccgtt
    21961 gtttgttgag ccggggaaac ggccggagcc tgttttgccg gagccggaac aggcgttttt
    22021 gcggctgcgg gcgaaagagc cgtccgggcc ggacctggaa caaccgttcc ggccggtgcc
    22081 ggttggacct tgcctttaac gggggctaca gactttggag caggcgaggc cgtattttgg
    22141 gcctctgccg caaaagcaaa aaaagcagtg gtcaacagga tcaggctaca attaatgaaa
    22201 atcaaaaata tttttttcat tgtgtttttt cacttgtctt ttctttttta tcaataaaca
    22261 tgtatgtttt tatcgtacag gatccggtaa caagatggcc tttgtctttg atttcttttc
    22321 ggtctccgat tgtaatgtcg gatacattaa cgattctggg cagtttggaa actttgtcga
    22381 aaaaataaag gatattctgg tagcttccta aaaccgatat tccgacaggt atttcctcgt
    22441 agaaaggttc cgtagacgga gccgcgccat ctttcttttc agaagaatcc gccggttttt
    22501 gatcgcgttg atcagaaggt ttcagcaagg ctgcggtttt cgaagccgca ggtccctggg
    22561 attccaggga tttgggcacg gatgctttcg gttcaaataa tacaaattcc actccggcgt
    22621 ccctgcccgc tgacgcgacg gagtggaaaa gtgaaggtat ctcccgctgg tcgggaagtt
    22681 tttgtaacgc tatcttataa cttgcctgca acgcttcaac atcggccttg tatttgttaa
    22741 gctgcatagc gatctttttt tgctcattga ttttattctg catctccgta agttgcgtat
    22801 ttaaagtggt tcttttttcc atggcggaag acagaaaata aaaccagtaa aaataaccga
    22861 ttaataaaaa tccgacaatc acaatcaata cttttgtctt agggggaatt ttcttgatgg
    22921 tattgatgtc gatggccata tttcaaaacc cttttttcag aatacaggcg aaattaaact
    22981 gttggacagc attgccggag atttccgtct ttttggaaga gaccagatca acggattgta
    23041 ccggttcgaa ctgctcaatg cttttcatga aatttgcgac agcaatgtta tcccggccca
    23101 tcccctcaat attgagagaa tttcctttct gagtcagccg gacaagccag atgtctttcg
    23161 gcggaacaag catcgctaaa ttgtccagtg ttttaagagg cgtcaggcgg ttttcttcca
    23221 gagttgcaat gacgcctatt ttctgctcca gttctttttt atcttttttg aacttttcca
    23281 gatcgccaat tttcttattc aaatcggtta ttaaagcttc ggaatctttg atctgcgttt
    23341 ccaggttact gatcgaagat tttatccaga ttgttaccca ggcgagcaaa agaataaaca
    23401 acacgacgga tccggcgaca ataaatattt gtctcaggaa cgtatctttc ttggccttct
    23461 cacgataggg taaaagatta atcttaatca tttgtcgcct attttcctca agcccaggcc
    23521 gatagcggtt gctgcaatcg gcttgatgct gtctaaattt ttgacatcaa tttttcgttt
    23581 gttgtaatta atctttaaaa aaggatccat gatttcggtt ttaacattga gcaattccga
    23641 taaatgtttg gtgatgttgg gaattctgga cgagcccccg gcgagcaaca cctgcttgat
    23701 atcctcgccg ccaaacgttg aacggaaata gtcaatggac ctttcaattt ccatgcaaat
    23761 cggctgggca aattccagga tgttattttt cagatccagg ttgtcgtttt cgcttccctc
    23821 gggaaatcct tcgattttaa gcttttccgc ttcttcaaag gggatctgat atttttcctg
    23881 gatactttca gtgatggaat tgcctgccag cgcgaaatct cttgtgaaca ccgacattcc
    23941 ccctttaacc acattaatgc cggtggtgct tgctcctata ttgatcagaa caacgatttc
    24001 ctcgttgtca aattcataat tgacttcata taatgtttcc aggacaaacg gagccacgtc
    24061 aacgatttca acattctggc cggctgctgt tactgcttcc agataactgc ttacaatttc
    24121 ttttttcgct gcaacaatga caatatccat ctgattcgga ttgtaattgt tttcgccgag
    24181 aatttgaaaa tcataattga catcgtccat gttatcaaaa ggcagatatt tcccggcttc
    24241 atcgtggatc agttcacgca attccgtttc gttcatctgc tccagcgtca ctttcttgat
    24301 gatgacggaa tttcccgcca gagacgtgac aacgccttta cggccgcaat gggcaatctt
    24361 gaaaagctcc ttgatttttg aggatagcac gggtgcatcg gcgagaatgc cgtcaataat
    24421 gatgcctttg ggcaggggaa cttcatggaa acggttcaga tagtatcctt tggcgttgct
    24481 gatgatttcc gcaagtttta cggagcttga accaatatcc aaaccgacga gactcttttt
    24541 tccggaaaat atttctttca aatccaccat caaagcacca taaattggtt atcgggaagt
    24601 catctttact ttgcccaccg gtaaacaata tctccttttt taaccattcc caattcgtta
    24661 cgggctatca tttctatgta attaatgtcg ttgcgcaata aaataatttt cttttttaat
    24721 gcgttgtttt ctgctgttat ggcgctgttt tgcgatttta attccgccag ccttttgctc
    24781 atcaagtaat tgtcaacgag acccctgttg ccgaatgtga tcaaaagcga cattaacagc
    24841 aaaaatataa ttagcaatct gccgacaatc ttcatcgaaa ttcaaaacta ctccggtacg
    24901 gaaatgattg aatatattat tcgccttcat caagggaata cttcttcatg tggcctttgc
    24961 agtgctttag cgaccttgag ttcagaattt tttctggtgg acaatccttt ttccattttg
    25021 gaaattgttt gaggtgttaa tccggcccgg cgagccagtt cggatatgct taaaggaatt
    25081 gtctcacgaa atttttttac gtgatttttc ttcatattat ctgccataga ttaaagcgac
    25141 acctcacttg cttctcataa ggaactaatt ttaaataaat gtcaagaatt ttttttaata
    25201 ttcattaata ttgcttaata ttcattaata ttgcttaata tcgattaatt ttgaaaaaga
    25261 acaatggcga gactgaacac gctaattcaa tgaataaaaa attgttaatt ctattgcgga
    25321 aaagaaatga tttataaaaa aagcgcaacg gtctgggacc ggatgttttt gagcatgtta
    25381 acggcaaacc ccttgctttt cggctatttt atgtaatttg caatttccca gttcgcaggc
    25441 cttttttgcg tcgagacaac cgtattgatg gtttcccagt gacagataat cagccccccg
    25501 gttgttgtag gccctggcgt tgtcgggttt cagcgctatg gctttgctgt attccgtaat
    25561 ggcctgctga tgttgaccaa gttgatcata agcaaaacca aggccgatgt aattgtcggc
    25621 ctctccggga ttgatgcgga tggattcgtt gaaatccgca atagccagct gatattgacc
    25681 ggtcgtgttg tataaggtcc ccctgttatg aaaagcctcg gcgtaatcag gtttgaggcg
    25741 gacagtctga ctgtaatctt tgatggcctg ctgatgccgg ccaaggttgt cgtatgcaat
    25801 tcccctgtta tagtatgcct cggcatcatt cggctgaagt ctgatggcgt tattcaaata
    25861 ctcgatggct ttttgcggat cactgaattt ttcaccatcc gataaagctt gagccttgtt
    25921 gaaccagtcc gacgtcgttt cgctcattgc cggggatccg agcaaagtaa agatacatag
    25981 aagtgtaatc atcatcttat acataaaaag gtcttcttcc gatttgcatt tcaacgcgga
    26041 tcatttattc cacccaggcc gtgagatttt ccgccggttc gcgctccgag cggacgtgat
    26101 tggcagggaa tgtttcgtcc ggatagccga cagcaatggc aatcatcagg cgcttgtttg
    26161 aagagatgcc ggtgcagtgg cggaccactt caggatacat gaccccctga tcttcaatac
    26221 aggtgccgac tccgaagtgc agcgccgtca gacagatgtt ttgcatgacc gcgccgaggt
    26281 ccatcaggga agcatgatcg cctaatattt tgtcgctgac aatgatgatg gccgccggag
    26341 catcaaaaaa acgaaatccc cgttccatcc accaggcgcg tttttccttg tcttcacggg
    26401 caatgtccat ggctttgaag agttgcttgg ccagcgtgat ctggcggtcc ttataaacgc
    26461 ttttatcagg ccagcccacc acctgatgat ccggtgtaat cggcgcaccg gacgtcagct
    26521 tgtcaacgat ggcggcacgc agtttgttga ggggctcgcc gctgacgaca atgaattccc
    26581 agggctgcgt attcatcgcc gacggcgcac ggcaggcgat attgagaatt tccatgatgg
    26641 tttctttggg caccggctga tccgtaaacg cccggatgct tctgcgatgc tgaatggctt
    26701 ctatcaaatc cattgtaaac ttttcctttt cctgtgttgt ccggaatgcc tcatcggttg
    26761 caaaaacaac ctcgccgttg aggtcagatt gtttaaatcg tttacgtctg aatgcagcgt
    26821 tgataacgtt gtcacaaatt tcgatgatga taatattggc ggaagtgcat gggaatcgaa
    26881 cccacctggg acggttttag cgccccacac cggatttgaa gtccgggagg cccaccagtg
    26941 accttggcac ttccgcggaa gaatctatcc ggaaagaaga aaagtgtcaa tcatcaaaag
    27001 tttatcagca gaattttcat ctcggatcgt attttcataa ttgcagagca tccgaccagg
    27061 ataacgaatc atcaagcaac tgaactgtga cctgggaatt ttttttgatt cgggtttcgt
    27121 tttctcccat gacaatgagg ccgttggccg tgaccatcga tttcaaaatg ccggagccct
    27181 gatcgccggt tgtcacggca atatatttgt ctttctcctt ccggacgacg gcacggatga
    27241 aatgtctaaa gccggcagct ttttcaaatt cttcgtggca gattgccgtg acggtctgac
    27301 ggaagatttt tttatggccc tgcattttca aaagcgaagg gcgcacaaat tgctcaaagg
    27361 aaaccattgc cgatacggga ttgccgggca ggccgaacag cggaacgcct tctatggcac
    27421 cgaacgccag gggtttcccc ggtctcatgg cgacctgcca gaaatgcatg gcattgccga
    27481 tttctcccat gacatttttt acgaaatcga aatcgcccac ggacacgccg ccggatgaga
    27541 taatgacatc ggcgttcagc gcttttttga attttttttc caaatcggat tttttatctc
    27601 tggcaatggc gaggatcaac gggatcgcgc cgcactccgc tacttgcgct gccagagaat
    27661 aactgttgct gttaacgatt ttgccgggcg gcgggttggt cctgatgctg accagttcat
    27721 cgcctgttga catgattgcc acgcgcggtt tttgataaac cttgagcgag gattttccca
    27781 gcgcggcaag catcccgata tgcgcgggac gcagcaagct gttttttggg atgaccgatt
    27841 cgcctttacg gacatcttca ccggcgaagc ggatgttttc ccccctcgcg gctgaagcga
    27901 aaatgatgac ggttctgccg tctttgcggg tgtcttcctg acggataacc gcgtccgccc
    27961 ccgggggaat cacggcgccg gtcatgatgc gggccgtttc gtttttgccg atgttctttc
    28021 ggggaaattt ccctgcggga atgtcttcga taattttcaa ttttacagga gttgtcttgc
    28081 ccgcgcccct ggtatcggcg caccgcacgg catagccgtc catggcggaa ttatcggcag
    28141 aggggatatt acgcggggac ataatatttt cgccgatgac gcgtcccctc gctttcagaa
    28201 gatggactgt ttccaggccc agcggctgga aattaaccag aattgttttt aaggcttttt
    28261 ctacaggaat catattcaat ctccgcggaa ttgatgcgtt ccagtttatc aaagatgccg
    28321 cgcttgtcaa tataaagata gatttttaat ggcaattttc ggaaaacaag ttttcaattg
    28381 aattatccgg gaaagaagaa tagaacacgt gcaaacatac ccgttccgga tcaaaagaac
    28441 tttttggatg cggaatgggt tttggtgttg tttgattttt tgggggattg attgccgtgc
    28501 ggaaaatgat cacaaggaaa aacccggaag atatggagtc gtttcgtctt ctgctgaaac
    28561 agatcgatga cgggatgatg cgcatcgccg tacagggcct tgccggcgcg gcccgttctt
    28621 ttctgatctc cttattgtat aaaaaacgac agaggcctgt actggtggtc tgccccgagg
    28681 agaaggaagc ggtggccttt gctcgcgatt tgtcgctgtt tctgggtgaa gagcacgtgt
    28741 ttcattatcc ggcgctggat ttcctgacca ttgacatgtt tgctcttcag cgtgacgagg
    28801 aattgtcccg tctggaggtg ttggccaact tgcagattca acacaaaatt attgtcgttg
    28861 cctctgcaaa tgcgttgatg cagaaagtga tgccggtggc cgagttcagc aagcatctgc
    28921 gaattattgc tcccggcgac accatcaacc gggatgaatt tgcagcgcag cttgtcgcgc
    28981 aatgctataa acgagaaagt ctggttcagg aaaaagggga attcagcatc cgcggaaaca
    29041 tcattgacat ttttcctccc accgaaaaaa atccgctgcg cctggagatg ctgggcgatg
    29101 aaatagagtc catcaggaca ttcaacacat cttcccagcg ttctatcgga ccgttgcccg
    29161 cttttgtatt atctcctgcc ggagaggccg tcatggatca ttcgacgctg gagctggcag
    29221 tgcgcaacat ccggcgccgt gccgacgaac tttccttttc ccgcgacatt cgcagccgtc
    29281 tggttgccgc cgttcaggag gggttgacca atgccttcaa tccgatattc ttgccgctgt
    29341 tttatgaatc gtacgatacc ggaagcggcc tttccgataa taccttgtcc ggccttttgg
    29401 aatatctttc aagaaataca ctggtcgttc tggatgatcc gctggctgtt gggcacgcga
    29461 tgttcaacgc tgggttggct atggataaat tgctgttcaa ggcaaagagc ggtggaaaat
    29521 tctatctgga aaaggaaaat acctatttcc cgccggaggc tgtaacggcc ggcctggatg
    29581 gcttcctaca ggttgcgctg gctggactcg aaatcgaatc tgaaagatat catgtgccgg
    29641 tagtttttga aagcgaccgg ttcacctgca caggggagcc gctggcaggt gaaatccgag
    29701 aggatgcaac gctacggttg accgtagaaa aaataaaatc atggtttgcc ggggacatgc
    29761 tggtttttct tttctgtccg ttgccggaag atgttcaacg gatgaagggg ttgctttcgt
    29821 cttacgatct gccggcggag acattgtccg gcgctgaacc ggttctggac agcctggaag
    29881 gttggaaagg cgatgcacgg ttgattttat tcgtgggaga catttcatca ggctttgtca
    29941 tgccggcgat aaagctggcc gtcttaagcg aagaagaaat cttcgtcaaa aaagcccgcc
    30001 gtctgcgctc gcggccagta cgcacggggt attttatcaa atcgttcggc gatctgcaag
    30061 aaggcgattt tgtcgtgcat acggatttcg gcatcggcat ttaccgcggt ttgaaaaaaa
    30121 tcgccgtgag aaagattgaa aacgattttc tggtcattga atattcggag ggcgataaat
    30181 tatacattcc cgttaatgct ctggagaaga ttcaacgcta tgtcggtccc gacggctatg
    30241 tgccgaagat cgaccggatg ggcggaacat cctgggaggc tgtcaaagaa agggtgaaaa
    30301 aatcggtccg cgactatgcc gaagaactgg tgtccattta tgccgcccgc gagacgctgg
    30361 aacgaaaatc gtttgccccg ccggacagaa tctacgaaga gttttgctcg acctttgaat
    30421 ttgaagagac gccggatcag gtgaaagcca tcgaagacat tcatcaggat atggatgacg
    30481 tcaagccgat ggaccgcctg atctgcggcg atgccggatt cggcaaaacc gaagtggcga
    30541 tccgctcggc gtttcgcgcg gtgatggacg gtaaacaagt tgctgttctg accccgacga
    30601 cgattcttgc ggaacagcac tatcagacgt ttacccgccg ttttgaagat tttccggtgc
    30661 gggtgggact gctgaatcgt tttaaaaaac ccgccgaaca gaaaaaaatt gtcgaagagc
    30721 ttaaaaatca gacaatcgat attgtcgtcg ggacgcatcg tcttttgcaa aaggacgttg
    30781 cgtttcaaga tctcgggctg gtcattatcg atgaggagca gcgtttcggc gtggcgcata
    30841 aggaaaagtt gaaaaaaatg cgcacgctgg tcgatgtgtt gacgctctcg gcgacgccga
    30901 ttcccaggac gctgcatctg tcgctggtcg gcatccggga tttgtccgtc atcaacacgc
    30961 cgcccaagga ccgggtttcc atcaaaacct atgtcatgga gttcgatgag gatgcgatta
    31021 aaacggcggt tgaaaaagaa ctggcgcgtc aggggcaggt gttttttgtc cacgaccggg
    31081 tgcgttcgat ttatgcgatt gcccgcctgg tgcagcggct cgtgccgcac gccaaagtcg
    31141 gagttgttca cgggcagatg aagccggcgg aaattgaaaa aaccatggcc gggttcatcc
    31201 ggcaagagaa caatgtgctg gtttgtacaa cgatcatcgg ttccggtctc gatatttcga
    31261 cggccaatac gattattatc aaccgcgctg aaaagttcgg cctggcgcag ctctatcaga
    31321 tacgcggccg cgtgggacga tccagccggg aggcgtttgc ctatctgttg ctgcctcagg
    31381 gagcgatact ttcccgcgca gcgatgaaac ggctgcaggt cattaaggaa ttttcggatc
    31441 cgggctccgg tttccggatt gcatacaacg atctggaaat ccggggcggc ggaagcgtac
    31501 tggggatgtc ccagtcggga catatttccg ccgttggtta cgagctttat actgaattaa
    31561 tcgaaaaaac cattcgcgaa gtcaaggggg agaaaaccga agaagactgg atgccggaaa
    31621 ttcagcttgg gatttccgca ttcatccccg aggattatgt gccggatgtc catcagcgtc
    31681 tgattttgta taaaagaata tccctggcgg caaacgatga agacatccgc gcgattgaaa
    31741 atgagttgct ggattgttac ggcgagatgc cccgtgttgc ggataattta ttgcgggtga
    31801 tctccatccg aaatgttctg aagccgctga aagggaagaa gatgggctat gacggtaaat
    31861 acctatacat cttcttccgg gagaattctc cggtcgatcc ggggaaaata atcgctcttt
    31921 accgcaagaa aatcaaagaa ttgcgtttta ccccggacca taaactgttt gtgccggcgc
    31981 cggctgctgc ggatatggac attctggttc aggcggatat gttgttaaag atgcttgccc
    32041 aatagcggta agcaggatat acagaaagat gatgattcca accagatggt tttctaaatt
    32101 ttattctttg gcgggaaaat ccaaaaaggt ttctccggtt cgctgcggtc ttttgtgtct
    32161 gctcttgccg gggttaatga tcgcctgcga taaaatggaa agcttttcgc gtccccatgt
    32221 cgccacggtc aacggtgcca aaatttatct tgatgaatac cagtcccttt tgaatgagaa
    32281 ggccaggatg ctttccggcg atttctttag acagcccgcg tatgtgaaaa aattcgagga
    32341 cgaagtcctg gatgccatga tcggggaaaa aataatgctc ttgcgtgccc gggaattgaa
    32401 cctgtcggtg agcgatgccg aactggaggc aaaaattaaa gaaatcgaaa aagattacgg
    32461 aaacgaattc accggtcttt ttgccagaca aaatatcaac tatgagaaat ggaaagagga
    32521 ttttcgaaag gaaattcttc tgcagaaact tattgcggcg gatgttcatt ctaaaattat
    32581 agtttccgaa acggaaatag aagattattt caaaaagcac cgtcacatct acaaaaggga
    32641 tttgtctgcc agagtgtcgc agattgtggt tcgggatatg caaaccgccg agcaggcgat
    32701 gcaacgattg aaatccaagg aagaatttgc ccgggtggct tcggagatgt ctatcggtcc
    32761 ggaagcctcg cgtggtggtg acctgggatt catcacgaaa cgggtcatgc cggaaccgct
    32821 ggatgaaacg atttttaaaa taccggtcca gacgatcagt ccgatcgtac agagttctta
    32881 tggatttcat attatcaagg tgatggaagt ccggcaggcc aaagaaagaa tcttgccgga
    32941 ggccagagaa gaagtgattg cggatattcg cctgctgaag gagaaaacgg cttttgcgga
    33001 atggctggaa gccttaaaga aaaaagccgt tgtccaaaaa aaagccgata ttaaaatcaa
    33061 tacatcatac aggtagcacg atcaagggag gtctgtggtg aaattttacg gaaaatatgt
    33121 ttttgcatta ctgggaatca tgcttttgtg tccgttattg cacgccgaag ttgtggataa
    33181 aatcattgcc gtggtcaatg atgaggtgat cacccggctt gagttgaatg cagccttcga
    33241 accgtatcgg aaaaatattg agaataccta ccagggtggc gacaaggacc aatttgttct
    33301 tcatgcgagg gaggaatttc tgaaccggct gatcgacaat atcctgattg agcaggaggc
    33361 gaagaaaacg gggatcagtg cttccgtccg ggaagaagac gttatgggtg ttcttcagga
    33421 catgctgacc aaacagaata tcagtatgca ggattttttg aaaaaccttg ccaaggacgg
    33481 cagctccatt gaggaggtca aaaaagaaat acggtcacaa atgatccgta tgcgattatt
    33541 aagacgggaa gttaaagata agattatcgt taccgatgag gaaataggcg cctattacaa
    33601 tcagcaccgc aatgattacg agggtaaaga aagcgtgaga atgaaacagc ttttattgct
    33661 gctgccttct ggtgccaacg cggagacaaa aggaaaaatc cggaaagaag ctttgcacct
    33721 gagggaacgt atcatggcgg gagaatcttt tgacctactg gtttcaacgt attctcaggg
    33781 gccggcggcc gctcagggcg gggatgtcgg gtttattgag aaggggacca tcatcccgga
    33841 agttgaggcg gttgcgttca gccttgccgt ggatcaggtc agtaacgtga ttgaatccga
    33901 tgcgggtttt catatcataa aggtcgtgga taaacgcggc gccggattaa aaccggttgc
    33961 cgtggtccgg gaggagataa aagcaaaaat tgaagatgaa aaactggata aaaaatttga
    34021 ggaatggatc agtgccattc gcgcaaaatc gcatatagag atcaagaatt aaagcaaaga
    34081 ataacatgga cgcgatcaaa atcaggggcg ccgctcaaca taatttaaaa aatataagct
    34141 tggatattcc ccgggatcaa tttgtcatca ttaccggtgt gagcggttca ggcaaatcat
    34201 cgctggcttt cgacaccatc tatgctgaag ggcagaggcg gtatgtggag tcactttccg
    34261 cttatgcccg tcagtttatc ggacagatgg acaaaccgaa tgtggaatcc atcgacggct
    34321 tgtctcccgc cattgccatc gagcagcgta cggccagcca taatccccgt tccacggtgg
    34381 ggacggtgac ggaaatttac gattatttcc gcttgcttta tgctgccatt ggcgtctgcc
    34441 attgttaccg gtgcggccgg gaaataaaat cacagaccat cgacggcatt ttggaaaatg
    34501 ttcttgcctt gccggaaaac acgccgttca gtgttctggc gccgatggtg agagggaaaa
    34561 aaggcgaatt tcagaaggaa ttgaaaaaac tccagaagga ggggtttgcc cgtgtccgga
    34621 tcgacggaga aaacagggac ctggcggaag acatcagcct ggaaaagaat aaacgccacg
    34681 atattgacct tgtgattgac cggttggtgc tgaaaaacgg tatcagaaaa agattgcggg
    34741 attctctgga gatcgcgttg aagcatgccg aggggctggt gcggatcgtg accgccggcg
    34801 gcgaagaatt tcttttcagc gaaaaatatg cctgccccga ttgcgggatc agcttgcccg
    34861 ctctgacacc gcgcatgttt tcatttaata atccctacgg agcctgtccc gcctgcaacg
    34921 gtctgggttc ttccatgcgt tttacggaat atctgattgt tcccgatgcc ggattgtctc
    34981 tgcgggacgg cgcaatagcg ccgtgggccg gtcgtcactc gctttactat tacagcattc
    35041 tggacgcgct cagcagccat tacggttttg atgtgaacac gccgttcaat aagctgccgg
    35101 aaaaaattcg gaaagttctt ctttacgggt cgggcagcga aacaatcaaa ttctatcatg
    35161 atcgcggcaa taaaagatat tttgttcacc ggccgtttga gggggcaatc cggcaattgg
    35221 agcggcggtg gcgggaaacc gcgtcaacgg cgattcgcaa tgatctgatg cgttatatcg
    35281 atttgagcgc ctgcgagacc tgccacgggg cgcgtttgaa agaagagatt ctggccgtta
    35341 cggtgatggg aaaaaatatc tatgaattgt gcaaaatgtc catccgcgat tgccacagct
    35401 ttttcgaacg gatcgaactc tcaccacagg aactcaatat taccgaaaga atcatccggg
    35461 aaatcaaaag ccggttgcag tttctgctca atgtcggaat ggattatttg aacctggagc
    35521 gagccacctc aacattatcc ggcggggaga gccagcgcat tcggctggcc acgcagatcg
    35581 gttccgggct gacaggcgtt ttgtatgttc tggatgaacc tacggtaggg ctgcatcaga
    35641 aagataatct gcgtttgatc gatacgctta agaaactgcg cgagatgggc aacacggtgc
    35701 tggttgtcga gcacgatgcc gacatgatca tggcctgcga ccatatcgta gatatcggac
    35761 cgggggccgg aacggagggg ggagaaatca tttttcaggg cacgccggta cagattctga
    35821 acagtgaaac gtctctgacc ggcaggtatt tgtcgaaaaa agaatcgatc aaccttccct
    35881 cagaaaaacg tccgcttacc ggaaggcaca ttattcttga aggcgccagc gaaaacaacc
    35941 tgaaaaatat cgatatcaaa ataccggtgg gtgtgtttac ggcggtaacc ggtgtttcgg
    36001 gatccgggaa gagtaccatg gtgattgaaa cactctacaa agtgctggcg cgccgtttga
    36061 atcagtacaa cggatccatg gggaaaatca aaagggttgt ggatcttggc gggattgaac
    36121 gcgtcattat gatcaatcaa caccccattg gccgcacgcc gcgctccaat ccggcaacct
    36181 acacgggcgt cttttcgttt atccgcgatc tgttcaccaa tcttccggag tccagaatgc
    36241 gcggatacaa accggggcgc ttcagcttta acgtgaaggg cggacgctgc gaggcgtgcg
    36301 aaggaaacgg tttaatcaaa atcgagatgc attttctgcc ggatgtttac gtgacctgcg
    36361 aagcctgtca tgggaaaaga tttaatcgcg acacccttga tattaaatac aaggataaaa
    36421 gcatagccga tgttctggac atgacggtca atcaggcgtt gacctttttt tccaatattc
    36481 cttccatccg gtcgcatctg cagttgctcg gcgatgtcgg gctgggatat attcatctcg
    36541 gacagtcggc gacgacacta tccggaggag aggcgcagcg gattaaattg gctcgggaac
    36601 tgagcaagaa agtgaacagc aataccctgt atatcctgga cgaaccgacc attggtctgc
    36661 attttgccga tatccagaaa ctgctggatg ttttgatgcg cctggtgaat atgggcaata
    36721 ccatggttgt gatcgagcat aacctggacg ttatcaagtg tgcggattac gtgatcgatc
    36781 tcgggccgga aggcggctcc ggcggcggca gaattatttc gtcgggaacg gtcgagaaag
    36841 tagcgcgaga tgaaaaatcc gtgacgggca aatttttgag agatgtgatc acggcctgaa
    36901 aatatgtcct ttttgatcct gtcatttttc cctgcttgca gaaacattga gtgcttcctc
    36961 aggtcattcg ttgtctgaaa gtaaaaatat cttgacatcc tcatgagaat ggttttttat
    37021 tttataaaat tcttcagcgg tgtcatcagg aaaggatatt aacctgtcta acgattccgg
    37081 atatgagcat tgcatcttga ttgttgatga cgaagattca attctcaatg cttttaagcg
    37141 cattctggcc gacgaaaatt acgacgtgca tgtcgcaaac agcggtttgg agggattgaa
    37201 caaactgcgt tcggccacaa aaccctattc tattattatt tccgatcaaa gaatgcctga
    37261 aatgagcggt gttcagtttt tctcgaaggc aaaagaaatt tttccggatg ccatccgtat
    37321 tcttctgacc gggtatgcgg actccgacgc cattattgaa gccatcaata aaggcggcgt
    37381 acatctttac ttcaccaagc cctggcatga agaagaaatt ctgctgcata ttaaacaatc
    37441 gctttccaaa gttgagattt tagcggaaaa taagcgtctg gtggaattga taagagagaa
    37501 aaataaagag ctgacggagt taaacaaggc tctggagcaa aaggctctgg agaaaaccaa
    37561 tgaccttttg attcaaacgg aaaaactcaa ggaaagttat caaaaatcgc agatcattat
    37621 ggacggcatc gtcagaacgc tgtccaaaat tatagagacg cgggatcctt atacggccgg
    37681 ccacgaagac caggtggcca aaattgcctg caagattgcc cgggagatga aattacccga
    37741 ggagcagatt tcgtttattc atattgccgc caccctccat gatatcggga aaatatgcgt
    37801 tccgtcggaa atattaacaa agcccagtgc attaaacaat ctggaaaagg ccatcatcaa
    37861 aacgcattgc aaagtggcca acgatgtgct ggcgaacata gaatttccct atccggtggc
    37921 ggaggtcatc tatcagcatc atgaacgatt ggacggatcg gggtatccga gggggttgag
    37981 gggaaacgag atcgcgctgg aagcccgcat tatcggcgtg gcggatgtca ttgatgccat
    38041 ggcttcctac aggccgtacc gtccggctct gggcgtggat gcggctatcg aggaaatcgt
    38101 caaataccgg gggatcacgt atgatcccga tgtcgtggat gcctgtctga cggtctataa
    38161 aaaaaccgcc cgaacgttga tcgagaccaa ccaccgcacc gtgaattgat ggccgtcttt
    38221 tcagcgcctg ggatttttac tcaattcctt tctccgaaaa ttgctctgcc gatgcgcacc
    38281 agagttgcgc cttcttcaac ggctacatca aaatcatccg tcattcccat ggaaagctct
    38341 tccatgtgta tgccggcgat tcccgcccgg atgatattct gttgaagatt tttaagttcg
    38401 cgaaaataag gacgcgatgt ttcgggatcg tccgaatacg gcgccatggt catcaatcct
    38461 tttaccgcga tatgctgcat ggcggaaaga tcctgaagca gcgcaaccgc ctttccagcc
    38521 ggaataccgt tttttgtttt ttcaccgctg acgttgattt caatcagaat gttgatttga
    38581 cgacccgccc gcccggctct tttatcgatt tccgcagcca ggtccagacg gtcgacggaa
    38641 tgaacgtagt cgaaaagaat caccgcatat ttcgctttgt tcgtctgcag atgcccgatc
    38701 atgtgccacg gaatgcttct gccgatgacg gcgatttttt ctctggcttc ctgaacgtaa
    38761 ttttctccca gggcggttat gcctgcccgg atcgcttctt cgatgaccgg aacggggacc
    38821 gttttggtaa ccgccagcag tttgacggaa tcaggagacc gtttgcctct ttcggcggcg
    38881 gcagcgattc tctgtttgac agcggcgata ttggcggcaa tgtctgttcc catatggcta
    38941 acaaaaatcg atgacgtgca gttttttcag cgtttccaaa acttcgcaga gcggcagtcc
    39001 gatcacattg gtgtaggaac cgcgaatgga gcggacaaaa caggcgcctt ggccttgaag
    39061 ggcatagcct ccggctttgt cgtaaggttc gtcaccggcg atgtaccact ccatttcgtc
    39121 cggatgaatg gttttgaagc gtacaacgga ctgaaccacc ccggtcgagc gaattccaaa
    39181 agacagccgg acaacggtaa atcctgtaaa gaccgtgtgc tgtctgccgc ttaatttttt
    39241 cagcatcgtt ttcgcctgtg ttttattgcg gggcttgccc agaaccgtgc cgtcgatgac
    39301 aacaatggta tcggctccca gaacacaggc gtcggggttc tcgacagcca ccttcatcgc
    39361 tttcaggctg gaaagcctgc gcacgtgttg gcgaggcgtt tccccggtga gatatttttc
    39421 attgacatgg gccggaatga ttttgacatg cacaccggcg gaccggagca tttcctgccg
    39481 tcgcggtgac gccgacgcta aaatcagtga cgcatgggct gtgatggaca ttcattcccc
    39541 ataatagaat ttttttcttg tatcacaaat ttaaccgttg ctgtcaattg caattatgat
    39601 tgcaatccgc cgggtattgg agttatgatg ttcgcaaata aaaccggtgt tttggggaat
    39661 gaagaaaaaa atcattatat ttgccgttct gatgcttctg ggcgtttttg tcagcgcggc
    39721 cgacgcgggc gaggtcgtga tgcagttgac cgtgcctgga tgtgccgcct gaggggccaa
    39781 gcaaaggata ggggctatcc tgaacggaat tcaaggcgtc aaacactatc aattcgagga
    39841 agggaatgtc gtgtccatta cctttgacga tcagagaacg tctgtggata tcattaccgg
    39901 agcactcgaa agaaaaggat tccccctcaa gggccgtccg gtatactgga aacgccgtta
    39961 aaggtatttt ttcagttggc ctgcttccag tctttcaaca atctttttaa catcctgaga
    40021 acgtcctttt ttacagatca gcagggcatc cggcgtttcg acgatcatca gatccctgac
    40081 tcccagtaag gcggtcaatt ttcgcggatt atagatgagc gatcctttcg aatcttcgag
    40141 aacggttctg ctcccgctga ggatgacatt attctgcttg tcttttgggg agatgtccca
    40201 taaagcatcc caactgccga cgtcgctcca gccgaaattt cccggcagga taaaaacgtt
    40261 accggctttt tccatcacgc cgtaatcgat ggagacggag cccagttttt tgtaaacgcg
    40321 ggacacggtt gccgtctcgc cggcggtgcc cagcgatgtg tcgataagtg tcagtcccgc
    40381 atataactct ggcagccagc gtttgatctc ttccagaatg accgaagtct gccagacaaa
    40441 catcccgctg ttccagtaga aattgccgct ccggacgaat tttgccgccg tgcgaaaatc
    40501 gggtttttca cggattgact taacgcggaa aacgtcttgg ttttttattg ttttgcataa
    40561 gattccccct tcgatataac caaaccctgt ctgaacactg gagggggtaa tgcctatggt
    40621 caccaatcct ttctcctggg ccgccacaac ggccgccgct ttcatcacgc tcagatactt
    40681 tccggaatcg gctatggcgt gatcggaagg cagaaccatc attgtatcgt ccgtcgatcg
    40741 cttacggata tgcaaggcgg ccagtccgat gcaggcggcg gtatttcggc ctaccggttc
    40801 gatcaggatg tttcgggggg gaatttccgg cagttgtttc attaacgccc gggcatgttt
    40861 tttacccgtc accacgagaa tatttgtcgg gggaaccaac ggctttatcc ggttaacggt
    40921 ttcctgaata atggtttttt cgcctgtgat gtccagaagg tgtttgggtc tcttttctct
    40981 gctgcggggc cagaatctga cgcctctgcc tccggccatg atcactgcgt acatgatttt
    41041 tctccctgtt tttttggcat taaaaataaa aatgatgaat gcttataata taatgtgtct
    41101 tgcaatttca aggcggaaaa tatattttat gccaccatga tcacgatcaa ctatccggaa
    41161 atcattctaa agaaagctcg agaagatgcc attctgcgcg gacatccctg gatattttcc
    41221 ggcgcgatag gctcggtaaa aggaaatccc ggccccggcg atattgttct ggccagagat
    41281 gcggcaggcc atccgctggc cctgggcttt tttaatccgt cgacggatat cgcttttcgt
    41341 gttttgacta gaagatgcga cgaaaatatt tcaccgtatt tctggcagtc ccgtttgcat
    41401 gacgcatata aattgcggca gagacttatc aatgagcaga caaatgccta ccgtctgatt
    41461 aatgccgaag gggacggatt tcccggattg attgttgacg tctacaatac gactctggtt
    41521 ttttcgatcg ccgtcgccgg aatggagaaa cagaaaaatc atcttttaaa tgctctgatc
    41581 gcccagctca aaccaacaag aatttatgaa caaagtgaca gccattcacg caagctggag
    41641 ggattggaaa acagaagcgg catcgctttt ggtgaagatg aaaattctac cgtggaaatt
    41701 atggagaatg gtctgaaatt cgaagttgat gttgtttccg gtcagaaaac cggatttttc
    41761 ctcgatcagc gggtgaaccg ggaaaaaatc ggttccctgg gccgtgatgc tcatgtgctg
    41821 aattgttttg cctataccgc ggctttttcc gtttattgtg cggaaggcgg cgccaaaaag
    41881 gttgtttctc ttgatatatc caaaacggcg tgcgttgccg ccagaaaaaa cctgcatctc
    41941 aactggtttt ctgtggagaa ttatccggtg atcgagaccg atgtgtttga ttatttcaga
    42001 catacgccgg aaaactacaa catgattatt cttgatccgc ccgcttttgc caaaaccaaa
    42061 agagatgtgc ccaaagcggc aagagggtat aaagacatta atttacaggc aatcaaacat
    42121 ctgagcaaag gcgggatgct ggccactttt tcctgttcca atttcattga agaagatctt
    42181 ttttataaga tcgttctggg tgccgcgcgg gatgccggtg ccgagttgca gcttttatca
    42241 aggctggacg ccggaccgga tcatccggtc gctttcggac accccgaagg acgttatttg
    42301 aaaggtttgc tgctggtcaa aagaaattga caatatttta tctgccatcg gaatcagttc
    42361 ttcgccagat tgccgataat tctgtcatgg atcagacggg aagccatggt attgccgaat
    42421 gctccgacaa agactgaaac ggaggtcata tctctgccga catactcaac tttgatggtg
    42481 actttttcgt cctgaatgac ggatgtaatg acgccggtgg aaattttacg ttcttttcgg
    42541 atacttttcg ctttcagatc ggtcacggat tgttcacagg cctgccagac cgcatcgata
    42601 tcggctttgt aatcgctgac cagatgtcca tcctggtaga aaaacctgcc ggaacttatg
    42661 ccggcgacct tcccctgtaa attcaccgcc gcaccgcaac ctgcaagtaa taagcacatt
    42721 ataaagaaga aaacggttat cgtatttttc atgatatcac cttccggagc gcttcaaata
    42781 atctgttgac gatcaatggc cgcggcatac ctgtataagg ggcgttacct ttgagcggct
    42841 ttcctttcgc gcaggttcat gatctctgtc cgagtcagaa tggaagcgat gttgtccatg
    42901 tgcttcatga tattttcccg gcccccttct tcccggtcga caagcgttat caccatgaca
    42961 acggacagtc cggctttgcg ggcctgttca atggccgtga ttgtggatcc gccggtcgtg
    43021 atcacatcgt cgataatggc aactttttct cccggccgga cactgccttc aatggcgctt
    43081 ttagtaccgt gatccttggc gtcctttctc acgataaacg atttgatcgg tttgcctttc
    43141 tggtaggaga taacggcaag cgcattggcg atgggatcgg ctcccagggt cagccctcct
    43201 gcggccgtaa tatcggtatc tttgagcatg tcaaaaatga tggtgccgat cagattcatc
    43261 ccctcggggt ccagggtcgt cggtttacag ttaaaataat aattgctttg cctgccggag
    43321 gcgagcgtaa aagggggatt gtcgctgtat ttgaaagacc tttcgagaat gatttctccc
    43381 agtcgttcct tcatttgatc aatggtcatt gtggttcctc cgttcggatg aaatttatga
    43441 ttgagggtga tgttgtaaca taaacttgat cggcagttca acccaggcgg tgaatggatt
    43501 tccagctttc ttcgccggtt cgaatttcca gggcttgacg gcctgtattg cagattgatc
    43561 cagaatagcg tatccggatg atttccggat cttgacgtcg cccacgcgtc cggtcggcag
    43621 aatttcggcg aagaccagaa cgatgccctc atatccgcgc aaacgtgcca tttccggata
    43681 aacgggaggg gtattctccc ggtacagcgg ataggcaatg gcgatgtcgg acatgggatg
    43741 ttgggcaact gcctcgaagc cgccgctgtg acgtgcctca acggatgatt tgtttatttc
    43801 ggggcggttt tcgtattcgg aaaatgtact ttcgacagcc ggagccgttc gggacgccaa
    43861 aggaagctga acggtgccga cggggctcgc cggccgttcg acggacgtca tccgtccggt
    43921 gttttttttc tgtcttatcg tttgctgccc ggtttgcacg gcttttcccg tgtcggcttt
    43981 tttattgtcg aaagacaccc agaccatatt caaaacattt cggtcagacg gcgccggcgc
    44041 accaccggac aactcgaacc cgataagaac cgctgccgcc gaaaaatgaa tcagcacgga
    44101 aaaagccaag gcccaaatga gagcgatttg ttgatgagcg gaatgcctgt atttcatttt
    44161 ttccccgaac gattacggtt tgattatttt ttcaacaact tttacggcct gaacaccgtc
    44221 acgggcatag aatgcgccga tggattttgc gtattgatcc gtaaccactg caccgccgat
    44281 caggaagtct gtgcggattc cctgcgcgcg tgccagcgca ataacctcct tcatgttgac
    44341 catagtggtg gtcatcagcg cggaaaggcc gatgacatcc ggtttttcct tttttgcagc
    44401 atctaaaata gcctgttcgg acacgtcttt gccgaggtca ataacacgat aaccgtagtt
    44461 tctcaacagc agcgcaacga tgttcttccc gatgtcgtga acatcgccgc gaaccgtcgc
    44521 catcagaacg gttcccctgg tcgttattgt cgctttctgc aaatacggat caaggtgcga
    44581 aagcgctttt ttcatcgctt ccgccgccgc catcaactgc ggcagaaaat aaattttttt
    44641 ctcgtagaga tcgccgacac gcacaatgga ggggatcata taatcatcga ccagttgaga
    44701 cgccgttttg ccggatttca ttgcttcgtc aagcaatgcg gtgatgtcgt ctttgttgcc
    44761 ttccagaatg gcctgaacaa ttttttctcc cgctgaaacg gctgtcgaag ggagtcctgc
    44821 gacagcggga gtctgctgcg aaaaatagtt gatgaagtga aacgcggctc tttcccgggc
    44881 ggtcaaggcg tccccggcat ttttaacatt catgaactcg acgctggccg gattggcaat
    44941 ggccatggtc agcccgcagt gttgagccat cgccagaaag gcggcgttga tccagggacg
    45001 gcccggcatg ccgaaggaaa cattggaaag gccgagaatc gttttgcact ggaaaatgtt
    45061 tttacaccat tgaatcgttt cgagcgtttc ccgcgcggcg tcggcatcgg acgcaatggc
    45121 catggtcagg ccgtcgacga cgaaatcctt tttcgtaaaa ccgaacgctt gcgctttgcg
    45181 aaatatcctg tcgatcacga cgcgccgctt tttcgccgtc cgggggattt ccccgccggt
    45241 cagcggcagc aaaataaaca ttgcgccgta tcgggcggcc aggggcagga gcctggccag
    45301 tttttctttt tccccggaga tggaattaat catcattctt cccggataga gtctcagggc
    45361 ggcctcaatg gtttcaatcc tggacgaatc gatgaccagc ggcagactgc tgacggtgga
    45421 caacagactg atcacgttcc ggatcgtttg aacctcatca atgccgggct gaccgacatt
    45481 gatgtccaga agaaccgcgc cctggttttc ctgatccagg gccatctgac ggatgatgga
    45541 taatttgccc tccaagagtt cctgctgcag cgatttttta ccggtgggat tgattctttc
    45601 cccgacgata tacaacgggt cattgtcttt aatttccagg aatctgcgtg ccgagctgag
    45661 cgcacaaacg gattttgtaa gcggcaaaac gggtttctta cgcgtgaccg acatcctgag
    45721 cgcgcgaata tgttcgggcg ttgttccaca gcagccgccc agcatattgg cgcctgcagc
    45781 cgccagcttg acgctgaaat cggcaaatgt cgcgggatcc atttcaaaaa cggtttgacc
    45841 gtctttcaga agaggcatgc cggcattggg cttggccaca agcggcaccg tggcgtacgg
    45901 tttcatcgcc gagatgaaat caatcatttg ttccggaccg gccgaacaat tgcagcccac
    45961 ggcgtctgcg cccagacttt gcaaagtgat gagcgcgctt tgtggcggtg ttccgcccag
    46021 cgtgtggccg tttttttcgt acgtcatgga tgcaatcgtg aaaacgtccg caagttcctt
    46081 gacggccagc agcgccgcgc gcgcttcctg aatgtcaatc atggtttcga tgacgatcag
    46141 atcgcatccg ccgtcaatta atcctcgggc ctgttccttg aacgcatcca ctgcttcttc
    46201 aaaagccagc gtgccgaagg gttcgacaaa caatccggtc ggaccgatgt ctccggcgac
    46261 gagtgtctga ttgccgcagg cttttctggc caattgtgcc agtttcagat tgacggcgta
    46321 aacatcgtgc tttgcgccga actgtttgag tttatagcga ttggctccaa aggtgcaggt
    46381 ataaataatc tgtgcaccgg ctttctgata cgatttgtgg acagcgccga tgatgtccgg
    46441 attgtccaga caccatattt ccgggcaaac acctgccggc aggcccagct tctggagctc
    46501 tgttccggta gcgccgtcca gaatcataat tttctttttt aaaaaatttc ttatattaat
    46561 aatttcaacc acggctatct cctgaaatgc aaagaatcag tttccggtga tcttatacat
    46621 caatggctgc ttttctgcaa ttttcatttc gttatttgac ggatgtgatg gatgttatgc
    46681 cagaatgaag cctttatgac gaaacaagat cagacaaaca tcggccacat gctcatcata
    46741 cagaatgcct ttgttctttt cgatttcttc cagagcggcc tctatcccca acgaagcgcg
    46801 gtagggccgg tgagaggcca tggattcaac aacgtcggcc acggcaagaa tgcgagcttc
    46861 gataagaata tcattccctt tcaagtttct gggatacccc gatccgtcca tcctttcgtg
    46921 atgctgataa acaatttctg ccagcggcca ggaggattcg acattcttca atatttcata
    46981 tccgcttcgc gaatgttcct gtatcagaga gtattcgagt ttggtcagcc tggtcggttt
    47041 tgccagaatc tccgccggga cggataattt cccgatgtca tggataacgc cagccatccg
    47101 gaggccgtcg attttatcct gggataatct catttccgcg gcaatggccc gggcaatgtc
    47161 tgtcacccgc aactgatgac ccgcggtgta gggatctctc atctctatgg cggacaccat
    47221 gacctggatg gtggtgccga ccgattttct cagactttcc agagtccgtt tcagttcgat
    47281 gtccattaat ttgcggtctg tgatatcttg caggataaaa ataacacatt cctgttcgtc
    47341 gatggtgatc gccctggcgg agtaaatacc gaaacgttcg gagccctccc tgcgccggaa
    47401 tttaaactcc cgatccagga tatccctgcc cgttttcaaa tccgcgatca ttgccttcct
    47461 ctctgttttg tccagccaga tgttcatggg cggttcgaac gatttggttc cgatgacctg
    47521 ttctcttttc catcccacaa tcttttcgaa tccttgattc acatcgatga tcagaccgtc
    47581 atgcaggcgg gcaatggcta tgcagtccgg cgtggtcata aaaattttat agaatttttc
    47641 ctccgattgg tgtgaaagtt cttccgccag cttgcgctcc gagatgttca ataaaattcc
    47701 atcgaaaatg acccggtccg gcaggcgtgt cggcacggat agcccctgaa accagataat
    47761 ctttccggat tggatggcga cgcgaccttc aaaattccag cggctggctg tctgggctgc
    47821 ctgggtaatg gatgacagaa atcgctcccg gtcatcttca tgaatacggg aaaggaattc
    47881 aggaaacacc gtatccaaat ggtccgtatc gaactttgac aatatttttg caaattcatc
    47941 gatcggttca ctgaggtagg ttattccgta tttgccgttc tgtgttgcat aaaactgaaa
    48001 aataaccccc ggaagatttt cagtgatgcc gcgcaatcgt tcatcttttt ccctcagcgc
    48061 tttttcaatt tgcttgcgtt cggtaatatt acgacacaca ttgacaacgg caaacggtgt
    48121 tccgtcttct ttgcgcaaaa catcgccgtt gatttcaaac aggattttcc gtccgtctct
    48181 ggtcatcaga tgatattctc tggggcccag tctgctttcc agcatgaggt gcagattgcg
    48241 aatgactctc tcgcgatcct ccggtgcaac gaagttaagc agattctgac ccagaaattc
    48301 atctttgctg taaccgccga tccgcagcgc attctcattt gcaaaaagaa tattgcctgc
    48361 caggtcggtg cgaacaacaa catccggaat tgcattgacc agtttgatgt gcagttcttc
    48421 acttttccgt aacgcttctt ccgccttttt gcgttccgta atatcgcgtg taatccctat
    48481 aatgccaatg ggcttgccat catccgacca caggaaagaa gcggtaactt ccgccagaaa
    48541 agtggttccg tccttgcgca actcttccga ttcaaacgta agcgtcgtat ttcgatcgaa
    48601 atcgggttca gaagaccggg ccaactgttc tgccatcatt gccgtatctt tggccagttg
    48661 ttggggtgtg accatatcct gcagggcaag cttcagatat tcgtcaaccg tccagccgtg
    48721 tgtgcgctcc acgacttcgt tgacatatgt aaaatgaccc gccaggtcca tggtccagat
    48781 caaatccggt atgcaggtgg taatcttacg ataccgttcc tcgctgcggc gtaattcttc
    48841 ctgtgccgct tccttctggg attctgtctg cttgtgcttt gcctgaattt ttgtttcgtt
    48901 cagctctctg gcaatgaccg ggcataggcg ggaatagttc tccttgacga tgtaattgcg
    48961 agtcccggga aagcggcatt cgatagtttt atctttgctg ctggcgccgg agatgacaat
    49021 caggggaatg tcggtttttt cttcctcgag aagagccatg gccgcgacag ctccgaattt
    49081 gaggtaattg ctgtcacaaa gaatgatatc ccatggtttg tcccggagag cttgccgcat
    49141 cgcggcagcg gtttctatcc gttcgtaaac cggatggtat ccgccttttt gaagttcgct
    49201 cacgatgcgc gacacgtcct tgtccgaatc gttcagcatt aaaacctgaa ggggctgcgg
    49261 attgccggtt ggaggggcat tatttttttt catacacctg ttcccctggg tgaaactgga
    49321 gtaaggatcg gactggttag aattctatca gttgcttttt gttttgctga ttgttatcag
    49381 ccttgatgag gcatccccta ctggtaaaca gcataagctt ttcatgtctg tttgtcaatt
    49441 gtgtaatcgt cggagaatca atcttggcac tgtaaggaag aacctttaac tttttgttgt
    49501 gcgtggcatt ttaacggcag attccgccaa tggcggtgac ggatttttcc ggtatgagaa
    49561 taaaattggg attgattttt acgccggcgt tctgcatgtg tagctgttta taaatgattc
    49621 cctggttttc cagatgaaag tcaccgtagc cggcggaaaa acggcgtggc aaaagcgttt
    49681 ggccttcccg catcagaagg tgattcagat aattcatgat ccagtccagt gcggcgtccg
    49741 tcatttcgct ggcggtggca tcataaacga ccgcggcggc cagatcgtcc tgccgggttt
    49801 ttgccttgat ggcgtccatg acggcatgcc ccgcagtcgc tcccatgaaa acggcctccc
    49861 ggcattcgcg aaggaaaatg gacagatgct cgctgctgaa atgtacgttg ttcgataaaa
    49921 aaatttgttt cccgtcgttc tttgtgacgg ccagccgcag gtacatcccc ttgaggacaa
    49981 taagggacgc ggcttcgcga atgaaaaggt cggtttgctt ttgctgatcc ggcgagacgg
    50041 cggttattct ctttttaaaa ccgagccgct gatatatttt ttcgcgggga ggttcaatcg
    50101 aaattttttc cagaaaaatg acggattcca tgctttcccc ttttgctcaa cgcgaagccg
    50161 gcctttaagg gcgaatcccg gccaaccgtt tgttaagatt cccggctacc gcgtctccca
    50221 ttgctgaagt gctgacaatt tttttgcccg gttgatcaat gtcgcgtgtc cggaggcctt
    50281 cctgtaaggt ttccgtaacg gcattttcta tcagggcggc ttccgccggc attgataaag
    50341 aatgccgcag catcagtgcc accgacaaaa tagcggccag cggattggcg atgttttgtc
    50401 ccgcaatgtc cggcgccgac ccgtgaatgg gttcgtacaa ggctgttttt tcccccagac
    50461 tggctgacgg aagcataccg atggatccgg tgagcatggc ggcttcatcg ctcaggatat
    50521 cgccgaacag gttcgtggtg acgataacgt cgaactgaga cggatcacgg atcagctgca
    50581 gggcgcaatt gtcgacatac atgtgtctca gcgtgacatc ggggtaattg cggctgacgt
    50641 cggtgaccac gttccgccag agttccatcg tttccaagat attggcttta tctacggaga
    50701 ggaggctctt tctgcgtgta cgggctattt cgaaagcgcg gacggcgatg cgacgaattt
    50761 catcttctga ataaatcatc gtgttgacgc ctttgcgttt tccgtttttc gatgtcttaa
    50821 tgccgcgcgg tttgccgaaa tagacatcgc ctgtcaattc gcgcacaatg ataatatcga
    50881 tgccgcggat gatgtctttt ttcagtggcg acgcatccag gagttcttca aaaacaacga
    50941 tcggcctcag gttggcgtaa atgcccaggc tgctgcgcaa gcccagcaac gccctctccg
    51001 gccggacact gtaagcgaga ttttcccact ggggaccgcc gaccgctccc agtaagaccg
    51061 cgtcgctttt cagggccagt ttcagactct cctccggcag aggcgttcca taacggtcat
    51121 acgccgttcc gccgacaagt ccttctgtga tttgaaagtc gacgtttttt ttgccggaaa
    51181 taatttttaa aaccttcagg gcttcgttta taatttcctg tccgatgccg tcgccggcaa
    51241 agacagcgat tttaaaatta ttatttttca tttttttttc ttttcccagc gatgtgttgc
    51301 atcagaccgc cattggcgat aagttcttcc atgaacggcg gaatggggtt gattttaaaa
    51361 aatttatccg tgccgtttat ggttatgatg ccgcgtgcac tgtccacggt gatttcattc
    51421 ccttctttca cggcatcgac aatatctttc gattcgaata tgggcagccc catgttgaag
    51481 gcattccggt aaaaaatgcg ggcaaaactt ttcgcgacga cacagggaac gccggccgct
    51541 tttatggaaa tgggcgcgtg ttcgcgcgac gagccgcaac cgaaattttc tccgcccaga
    51601 atcatatcgc cctttttcat ctttttcatg aagtccgggt cggcatcctc catacagtga
    51661 accgccagga cttcaggatc cgacgtcgtc agataacgcg ccggaatgat cgcgtcggta
    51721 tcaatattat ctccgaattt ccagactttt cctttaaata tcatgttaaa atccttttta
    51781 acgagactca atcttcagaa acaatgtgta tagaagattg taagttgtaa tgagtcttaa
    51841 attttaatga aactcagata ttacaggcgt agcgtagtaa tatctgcaag ttgaattatc
    51901 ctacgtgcga agctaagggg catgtgcgct ttcttacaat tcttccggag aggcaattct
    51961 tcccagtacg gctgaagcgg cggcaaccgc cggattggcc agatacactt cgcttttggt
    52021 atgacccatg cgaccgacaa aattccggtt tgttgtcgca accgcttttt ctccttccgc
    52081 caggatgccc atgtgtccgc ccagacaggc gccgcaggaa ggcgggctga tgacggcgtt
    52141 cgctttcaaa aaaatctcaa tcagcccttc ttttaacgcc tggcggtaaa tttcgggggt
    52201 tgccggaacg atgatcagtc tgacattcga ggaggccttc ctgtttttta aaatttttgc
    52261 ggcgattctc aggtcttcta tacgtccgtt ggtgcaggac ccgatcaggg actggtcgat
    52321 ttttacccgt cctgctttgc tcactccttt gacattggaa ggcaaatgcg ggaaggccac
    52381 ctgcggttcg atggtcgccg cgtttatttc aagagtgtcg gcataaacgg catcggggtc
    52441 ggaagaataa aatgtataat gtcgttttgc ctttttccgg atgtattctg cggtcacttc
    52501 gtcgggagca aagataccgt tttttgcgcc ggcttctatg gccatattgg ccatggaaaa
    52561 tctgtcggcc attgtgaggc gggcaatcgt ctctccggtg aattccatgg ccttatacaa
    52621 agcaccgtcc acgccgatgc ggccgatgat atgcaaaatc aaatcttttc ccgagaccca
    52681 tttctgcagt ttgccgaaga tgatgaattt gatcgagggc ggtaccttaa accacagttc
    52741 tccggtgagc atggctgccg ccaggtcggt gctgccgacg ccggtggcga atgcgcccag
    52801 agcgccgtac gtgcaggtat gactgtccgc accgataacc aggtctccgg gcagaacgat
    52861 cccctgttca ggcaataagg catgttcaac accgacaagg ccgccttcat aaaaatgcgt
    52921 tatcttctgt tgcgaggcaa attcgcgcac cgctttgcat tgctgggcgg aattaatgtc
    52981 cttattgggc gtaaagtgat ccaggacgag agccacctta tgtctgttga atactttttc
    53041 cccgcccgcc tgcttgaacg cgttgatggc tatgggagcg gtgacatcat tgcccagggc
    53101 aatatccact ctcgcgttga tcagatcgcc ggttgaaatt tttctcaggc cggtgtgcgc
    53161 cattaatatt ttttccgtaa tggtgttgcc cacgaggggt tctccttgta aaaagtgaaa
    53221 cggtctttag cgtattttaa ttcattattc aacaatttta acctttttaa tattattctt
    53281 taaaaattcc atgcgattca aggcgttgat ataagctttg gcggacgcaa caatgacatc
    53341 cggatgagcg ccgcgtccga cgaccgagta actgctgtgc ttgagttgca cggtgacctc
    53401 gccctgcgca tccgatcctc cggtgatggc gtttacaacg tacttcatca acggatagtt
    53461 ggttttggtt atgttgcgaa tggctgcgaa ggtggcgtcg accggaccgt tgccgaaacc
    53521 cgctttccgc acgatgtcct gacccacctg catttccatg gttgccgtgg gaatggccac
    53581 attgccgctg accacattca gataaagcag gctgtatttg tcttcgcccc ggtagatttc
    53641 ttcggacaga atgacctcga gatcttcatc gtaaatatct tttttgatat ctgccagttc
    53701 tttgacgcga gccgccacct tgttgatctg ctcatcattg agttggtatc ccatcttttt
    53761 caggtggtgt gaaatggcgt tgcgtcccga atgctttccc aggacaatat gtgtgtcgct
    53821 aatacccacg gattgcggcg tcatgatctc ataggtgatt ttttccttga tcaatccatc
    53881 ctgatggatc ccggattcat gggcgaacgc gttggcgccc acaatcgctt tgttgggctg
    53941 taccggtatg ccggtaatct gagtcagaag acgcgaggtc ttgtaaatct gattggtctt
    54001 gatgttcgtg tgaaggccga agagatcctt gcgggtttgc agtgccatga cgacttcttc
    54061 catggcggta ttgcccgccc gctcgccgat accgttgatg gtgcattcta cctgccgcgc
    54121 accctgtctg atggctgcca gtgaattggc cacggccagc cctagatcat tatggcaatg
    54181 cgtggcgatg atggcttcgc cgatattttg gacatgcgcg aagagatagg caatcagatt
    54241 gccgaattct tccggcgtgg catagccgac cgtatcgggg atattgacgg tgcaggctcc
    54301 cgcgtcaatc accgccgaaa ccatttcgac caggtaatcc ttgtccgaac gggtggcatc
    54361 catcggcgaa aattctacat tatcggtata agaacgtgcc agttttaccg cggcaaccgc
    54421 ttctttgagt acctgctcgc gactcttttt gagctgatac ttcagatgga tgtcggaaga
    54481 agaaataaaa gtgtgaatgc ggggatgtgc ggctcccttg atagcctgcc aggcgcggtc
    54541 aatgtcgagt ttattcgccc gggcaagacc cgccacttgg gattttttaa tttccttcgc
    54601 gatttgtctg acggcgtcaa agtcaccttc ggatgcgatg ggaaatcccg cttcaatgat
    54661 gtctacaccc agcctttcca gttggcgggc aagcatcagt ttctcttccg tgttcatgct
    54721 gtaacccggt gattgttcgc cgtctcttaa ggttgtatca aaaatgtaaa cacgattttt
    54781 cttttttttc ataatatctt ctcctctgat gactatatga atgatcccag taaccgaagg
    54841 ttcgtgggca atgtaccgta atttccccgc gtagtttcca tctatcgggc ggtatgccgc
    54901 tttttaaaaa acaaaaaagg ccgtggcttc cgcccacggc cttttaggtg atcagttgct
    54961 ttttctccta gtgatgacct aaaaacgatg ggcgggcggc ctgaggtaaa aggcgcaaca
    55021 aagcgagagc cagcagtaaa ggcagaatgt tgattgctat gaaattcttt ttcgctgttt
    55081 ccgtcatcgt ttttgcaatt ccaaaaattt tggaggcttt ataatgcaaa aaaagaatct
    55141 tgtcaaaatt ttttttgaat tcattaaaat aaaactgaca taaagaactg ataacaaatg
    55201 cactcccaaa gcggtagatg gcgtttaaca ggttacggat gatgcttttt ccccaccgga
    55261 tgatttttta atgctggaga tatgtcaata atacatttgc atgtaagaaa gaaatatact
    55321 aagaggacag cacaataaat ttttattcat tcgttacatg taaagaacaa tggctacaag
    55381 cacggatctg ttcttgagca ggttttttac atcaattaca aaaggggcta tttaaagatg
    55441 aagaaatttt ttttattacc gctggctgtt ttttttctcg ttgcagttgg tttaaattcc
    55501 tttgccgaag aaaaagcact tacgttgtct gcttctgaaa aactgctttt taactttgat
    55561 tttattcctg tcgtattaac ggcaagtccc gacgggaaac gtattgccta tgccacccgt
    55621 gtggaaggca agcagtttgt tattgtcgac ggcaaggcgc agaaacgcta tgacggtatc
    55681 ggttccaatg ttgttttcag ccccgacagc aagcgttctg catatcgagc ccaggttggc
    55741 aataaacagg ttgtggtaat cgacggtcaa gaagggaaac gctatgacac agtgggatca
    55801 aatatcgttt tcagtccgga cagtcaacgg gttgcctatg ccgccaaaac aggcaagcgg
    55861 tgttttattg tggtggacgg gaaggaaggc aaatcgtacg agggaggtat cggctccaat
    55921 attattttca gtccggacag taaaaaaatc gcttacgggg tgctatcaga taaaaagcag
    55981 tttgtcgttg tcgatcaaca agagggcact cattatgaca gtatcggcaa taatatcgtt
    56041 ttcagtcccg acagtcgccg gatggcttat caggctcagt cggataaaaa acgttttctg
    56101 gttgtcgatg gacgggaagg caagcgctat gatgatgtca gaggcgattt tgttttcagt
    56161 ccggacggca agcaggtagc ctacgtggta aaatcagccg gtaagtggca ggttgtcgtt
    56221 gatgagcagg aaggcaaata ttatgacggc atcggagatg atattttctt cagtccggac
    56281 agcagtcgga tagcgtatca agcgcaatcg gacaagaaac gttttcttgt ggtcaatgga
    56341 caggaaggta aaggatatga acgtatcgga tcaaccatcg ttttcagtcc ggacagcaaa
    56401 aaagtggctt atatcgctca gatcgtgaat ggcaagtttg cgtccgtcat tgacggtcag
    56461 gaggggaaac cgtacaacaa tatcggcaac gggtctcttg ttttcagccc ggacagcaag
    56521 cgtgtggcgt atgcagccca gtcaggacag aaacggtttg ttgtcgttga cgggaaagaa
    56581 gggaaacact atgatggagt gggaagttac acgctcgttt tcagtccgga cagcacaaaa
    56641 atcgtttatg ttgcgcaggc agaaaataaa caaatagccg ttgtaaatgg aaaggccggc
    56701 gaaaaagagg tcaataaata taacagcatc gttgctttgg ggaaaaaagg ttttgccttt
    56761 gagacatcag acagattccg ttttcttgcc gtaaaggaga aatccgtcta ttctgttgaa
    56821 gttaagataa attaaacgga ctcagggctg gaaaatcaat attcgtgtca gaggaagatc
    56881 ggattttatt tcaataaagt ttaaaaaatg ttaaactttt tgtctatacg ggatttttcc
    56941 acgcactaaa tttacctgca tgtttttaaa aaaatttact tgacaaaaac atctataaaa
    57001 attagattgc gaaattcgct tgagggtgta ggtgtgtttt tttacattct gatgcgcaag
    57061 ttattgaatt ttatattttt tgatgggatt ggctggcgta gctcaatcgg tagagcagct
    57121 gatttgtaat cagcaggttg cgggt
//
LOCUS       DAONQZ010000009        52064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_697_length_52064_cov_16.331423, whole genome shotgun sequence.
ACCESSION   DAONQZ010000009 DAONQZ010000000
VERSION     DAONQZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 52064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52064
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_52064_cov_16.331423"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1504
                     /locus_tag="PLR13_03080"
     CDS             377..1504
                     /locus_tag="PLR13_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HQC18345.1"
                     /translation="MKKHVLDEGLCTGCGACVGICPYQVIYHDRTVQLHGCDLVKGRC
                     YDYCPRTPCDVGAIRERYCAAGDLTPEIGAVKGYYFSRAADPGLRNIGQHGATVTAML
                     ELALSEGLIDAAVVSSRDQLSLRKGVIVQNREAIRTHAGSQFTVSATVAAFHQLVTKQ
                     TGQVGIVATPCQALALAKMKFNPGHEDIARVNQLTLVIGLYCGWTLSEEKYLKLLTDK
                     GIALESITEMDIPAGRNVLEIRTEDRLHSIAMEEAQTCIREACLYCMDSTAEFADVSV
                     GSARFSGNWEDVRQWNQLIVRTTKGKELTDLAVEKGVLELREAPADSLKELKEAAVNK
                     KKNALKNIIQKSGSAKNLLYLDSRDPAVLKYLEAEKKKSKK"
     gene            1808..2914
                     /locus_tag="PLR13_03085"
     CDS             1808..2914
                     /locus_tag="PLR13_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQC18346.1"
                     /translation="MSEDIAILIAYVLLALLVSFLCSIAEAVLLSITPSFIEEQKEKN
                     PKRAILLKRLKQDNVDRALAAILTLNTIAHTVGAIGAGAKATVVFGSAWFGIFSAVMT
                     LMILFLSEIVPKTIGAIYWPKLVVPTVFYVQTLIVTLFPIVWISEKMTKFISHGKNLH
                     IFSRDEFVAMARVGEQSGQIDDNEARIIKNLFRFGSLNVTDIMTPRTVIAALSEDKTV
                     ADAAEYVVKIPFSRIVIYKKDIDDITGFVLRDDVLLRDNQERGGEALKTLKREILVVP
                     ESAVLTDLLEIFLKERQHIAIIVDEYGGTTGLVTLEDLIETLMGLEIVDEMDTVVDMR
                     VLARKKWEERAGALGIKINVDGKDQSKKENVLPE"
     gene            2999..4942
                     /locus_tag="PLR13_03090"
     CDS             2999..4942
                     /locus_tag="PLR13_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQC18347.1"
                     /translation="MINKLLSPAKTEVLFMGNEAIARGALEAGVSVVSGYPGTPSSEV
                     IERLSEVARERNLYVEWSTNEKVALEVAAAASFSGLRSMCVMKQNGVNVASDFLLHLA
                     SSGTRGGIVLVECEDPGALSSVNEGESRYFARMLEIPMLEPANFQEAKDLTRWAFELS
                     EKIRNIVMVRSVTRMSHASGNVICGSLPAKFRNARFISDGSVMDPLRGPVVSTPVVIK
                     HGLQQKKLQTAAELFEKSPFNSYVGPKKPELLIITSSACFLYSREALEILGLQKRVGI
                     LKLSCTWPLPEKLLKKYLRLTKKIMIVEEVLPFLEENIKVAAMTMLKDIGIKTFYGKT
                     DKTLPSAGELNPEIVMRALSKIMKVKPKSLPAGYLKEVQKVAPKIAPRAQTFCPGCPH
                     RASFWNINTALKLDGREGIVCGDIGCYAMASLWPEIGFHTVRTLHSMGSGTGLASGFA
                     KLSAFGLDKPVMAVCGDSTFYHAVIPALINAQHNQADITFIVLDNAGTAMTGFQPHPG
                     INESAIGEPLPPVDIEAVCRAIGAKVKVSDPFDLEGTSRILTSFMAERGSMKVLIMKQ
                     ACALSPEKKGKKQYQMKVNEDLCLGDQCGCNRLCTRIFRCPALTWDRKKQKAVIDDVI
                     CAGCGVCYSICPQKAIARVEAEA"
     gene            4939..5550
                     /locus_tag="PLR13_03095"
     CDS             4939..5550
                     /locus_tag="PLR13_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HQC18348.1"
                     /translation="MKKSLKKNPTNIIITGVGGQGNVMASRVLAGMLVNAGFIVTIGE
                     TFGMSQRGGSVMSHLRVSSSSVLSPQIPQGRGDIVIALEPVEALRVLTKYGNPDVAVL
                     TNSRMVYPMGVITGEFTYPTIEEVKSMFEKISAKNWIIDATSVAVELGNPVLSNIVMI
                     GALAGTSLLPIDRRAFEKEIAKTIPAGKHQINLKAFDAGVKML"
     gene            5582..6325
                     /locus_tag="PLR13_03100"
     CDS             5582..6325
                     /locus_tag="PLR13_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18349.1"
                     /translation="MDADHGSSGKVTYNYRFIFKDGVEKEFNIALDSVTLNLLPTDKK
                     NYPEWTKLNCFRCSHCTLNDQCEYCPIAINMVDVVDYFRNYFSYDIVETIVTAQERKY
                     FRTVSLQHGVSSLIGIYMVTSGCPVMETLKPMVRFHLPFATIEETKYRAISMYLMAQY
                     FLYQHGRQPDWDLKKLAEAYENVRIVNEYFCKRLRMIQGQDASLNAVVLLDIFADSVN
                     FSMDGRMANDLEYLFKGYFESTPGANNAS"
     gene            complement(6398..7282)
                     /locus_tag="PLR13_03105"
     CDS             complement(6398..7282)
                     /locus_tag="PLR13_03105"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HQC18350.1"
                     /translation="MKAEDIKKILIAGAGTMGRSIGLSCVTSGYAIVLYDINDDIPAK
                     ARDIMNKKIDKMADFGMMPKDKAESFKNNITATTDLAAAGADADVVSESIPEDPDIKG
                     DFFAELNKVCPARTIFTTNASSLVPSMFADKTGRPEKFLAWHFHPGFKLVDVMGHPGT
                     SPETVETVQRFSELIGHSPIVLKQEKAGYVFNSLLNPWFLAALNLASRDIASPEDVDR
                     SWKEITASPLGPFEMMDYVGLETVWRITDFWAKKRNNHQAKLSADLLKKYVDRGELGM
                     KTGKGFYDYTQKKSPGES"
     gene            7608..9668
                     /locus_tag="PLR13_03110"
     CDS             7608..9668
                     /locus_tag="PLR13_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18351.1"
                     /translation="MSDKINIGPSGRNIIFCMVLTALILAAYLQVHQFDFVNIDDSVY
                     VTENDYIRSGLTLDGIRWAFTTLYAEFWHPLTWLSLMLDYELYGLHAGGYHLTNLFLH
                     IISSLLLFGMFQRMTGAFWPSAFVAAFFSLHPLRVESVAWVAERKDVLSVFFWMLTLY
                     FYALYTEKPVFRRYLAAVFFFACSLMSKPLTVTLPVIMMLLDYWPLKRFASRRQNLFL
                     WQLKEKLPFFLLSAVFSLIAIYAQPKFPIDGWPFSLESRIINAIISFVLYLQKLFWPY
                     DLAICYPFFGRAPAGQIIAAVLLFFAVSAAVVWMMKKSPCLLVGWLWFIITILPVIGI
                     IPAGNNAMADRYLYLPSIGIAVMIFFGIPLLVENEKIKKFFLPPAALIFFAVLSGMTW
                     HQCGFWKNSEALFRNASRINEENALAHINYGNALAGEGRLEEAVVYYNKAWGMLPETG
                     GNVMAYNKMGIAENKLRQLIDVSLGNVYTKQKKYQEAVHHYTRAIAMRPITPDHVRTY
                     NFRGTAYSRLGRKKDAIRDFNEAIRLDRRDYRAYNNRGLLYAETGMHRQALQDFNQAV
                     LMNPNNAELYKNRGIACASLKLYQNAFEDFEKALTLVPDDANIYYNRGVAYIELGRHQ
                     EAFSDFNEAIRLKADYTNAYHYRGGIYLMYGYAASGCDDARKACQLGNCEILEAAGSK
                     NLCR"
     gene            complement(9744..10685)
                     /locus_tag="PLR13_03115"
     CDS             complement(9744..10685)
                     /locus_tag="PLR13_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18352.1"
                     /translation="MRKDIRNPDIHSSPWLLDLAWFYIQRKMLGRKLPLLASFKITYR
                     CNLSCRACPFHLRSDREKEHMSRDAAVHIMDLLNQLGTRMIVFEGGEPLLWRDGSYRL
                     HDLILYARKKFLRVAVTTNGTMPLDVPSHTLWVSLDGQRETHNTLRSNSFDQVRANIQ
                     NTRHPRVFIHYTLNRRNWQDLPFVAQWVQDTPALKGMTVQFFYPYHQGEDDLSLPPEE
                     RRAAIEKLLQLKKSGLPILNSAGRLRAMIDNRWSCHDDILINVDPDGTITKGCYVKNR
                     GNINCDACGFTPVAEASGALDFIPGSLYAGWRLFLKM"
     gene            11037..12248
                     /locus_tag="PLR13_03120"
     CDS             11037..12248
                     /locus_tag="PLR13_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HQC18353.1"
                     /translation="MNDIYSAVKVTDHVYWVGAIDWTIRDFHGYTTPRGSTYNAYLVM
                     ADEITLIDTVKKPFMDEMLARIKSVVDPSKIKYIVSNHSELDHTGSLPDVIDFIKPQK
                     VFASGVGTKTLKDLFHDEREIIAVENGQSLNLGNMNLTFMETRMIHWPDSMFTYLDVD
                     ELLFSQDAFGMHLATLERFADEIPVSTLEYEGATYYANIVLPYSPIVLKALEKVAASG
                     LKISVIAPDHGPIWRKDIGWIIDLYKKWALQKPTAKAVVIYATMWHSTEKMARIISEA
                     LAQAGVKVSLISMNETHRSEAVYEVLDAGALIVGSPTLNNNILPQMADVMTYLKGLKP
                     VNKIGAAFGSFGWSGESVKHLEAMLKEMKVDIASESVAVKHVPGADELDQCQKLGKTV
                     AAKLLEKVNSQ"
     gene            12280..12438
                     /locus_tag="PLR13_03125"
     CDS             12280..12438
                     /locus_tag="PLR13_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQC18354.1"
                     /translation="MKKYVCGVCGYIYDPAEGDSEGNIPAGTPFEKLPENWVCPVCGA
                     AKDEFSPE"
     gene            12571..13065
                     /locus_tag="PLR13_03130"
     CDS             12571..13065
                     /locus_tag="PLR13_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQC18355.1"
                     /translation="MDIKEALSQAYTGEAKAALRLKVYAQKAEDEGYKQMAKLFRVIA
                     FSEEIHGARALKGLKEIKSTEENLAASFESETQVAEVAYDRFVKLAEAEGNKEAVLHF
                     SQSKDVEETHAKLYKQAMNHFMEERETDYFVCKICGYVADGVLPEECPVCGAKAKMFV
                     AFQG"
     gene            13146..13985
                     /locus_tag="PLR13_03135"
     CDS             13146..13985
                     /locus_tag="PLR13_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HQC18356.1"
                     /translation="MHIRYTVTVFLLIIYFSPGKAIGEEKPLWEIGAGMAFLQMPDYR
                     GSDESRFYPLPYPHVVYRGDFFKVDENRITGQIFKTDRVLLDFSIFGSVPVNSDDNAA
                     REGMDDLDPTFELGPALKIKLWEHKQHKTRMNLTLPARAFFSTDFSSVRHEGWVFSPR
                     INIVTDDLIPRTGLNLGVSAGPMFADSGYHEYFYEVEPRDARVTRPVYRADGGYSGST
                     VTIGLSKTYKHFIFHAFVSADFLQGASFEDSPLVKRETSMMSGFNVSWIFFKSEKMVN
                     AEK"
     gene            14050..14454
                     /locus_tag="PLR13_03140"
     CDS             14050..14454
                     /locus_tag="PLR13_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQC18357.1"
                     /translation="MIITRWLIITAAILLASQFVPGIEVDKLSTAVIAACVLGLINIF
                     IRPIVVILTLPLSILTLGVFYFFINAFLLELVAYFVSGFAIKNFFSAFAGSLIISIVS
                     WLANSFITSHKIVKKDDPDYIDLRKGDDGKWR"
     gene            14445..17102
                     /gene="mutS"
                     /locus_tag="PLR13_03145"
     CDS             14445..17102
                     /gene="mutS"
                     /locus_tag="PLR13_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQC18358.1"
                     /translation="MAVGATLDLTPAMKQYVKIKQQHPDCILLYRMGDFYEMFFEDAV
                     TAAPVLEITLTSRNKGKEDSVPLCGFPYHAASAYITKLVEKGFKVAICEQVEDPKKAK
                     GIVKREVIRIITPGLVVDEENLAPGENNYLACLCAEKNTLGLAFLDISTGEFQIGEFT
                     DRDYFFTVISGLDFKELLLPRNFPDRILLDTIEAQMPQLRINDLDENYFLTGQAGDVL
                     QRTFSTDVLDACGISEHPAALKASGAILSYVNETQKINPQHISAIRWYSAEKFLLLDD
                     TARRNLEIFTSIQNNTAAGSLFSLFSETQTPMGTRRLRWWMSYPLVDAEKIKTRLAAV
                     AELKDDPLRRANLRKTLSRIYDLERLAGRVSLKAANARDLVALKNSLLAVPEIKSLLS
                     DCTAPAICAIRSHITEMTDVVDVISRAIVDSPPQKIQDGGFIAAGYDEELDKLRLISR
                     DGKQWIAALEAEERKKTGINSLKVGFNNVFGYYIEVTKANAALVPDSYIRKQTLVNAE
                     RYINQALKEFEQTVLNAEERIKQKEQELFINLRDGLDRYIAEIQGNANLIADLDALAA
                     LADVAEKYHYVCPEIDDEETIDIQDGRHPVVESALKNEGFVPNDCLMDLRQNKLLVIT
                     GPNMAGKSTYIRQVALIVLLAQMGSFVPAAKAKIGIVDKIFTRIGASDSLIKGQSTFM
                     VEMTETAEILKNATPRSLVIIDEVGRGTSTFDGLSIAWAVAEYIHDFQGRGVRALFAT
                     HYHQLTDLVVTKPGAKNYNIAVKEWGEKIIFLRKIMEGGTSRSYGIEVARIAGVPADV
                     IMRAKEILRNLEKGEFDEIGMPRIVRGAREGKNANPQLSLFSRRETAIALEKKGQNLI
                     EALKNIDISSLSPLEALNKLNELKNKISD"
     gene            17572..18939
                     /locus_tag="PLR13_03150"
     CDS             17572..18939
                     /locus_tag="PLR13_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC18359.1"
                     /translation="MVVEKAVLIVDDDSVHRMILKKHLNDWGYQVNEADDGHVAVEAV
                     RRMPFDLIIMDICMLNVSGIEALGQIKAINPETPVIIMTAYASLETAVSALKKGAYDY
                     LTKPLNFDELRIAIGRATEHNRLKRENRYLRNRLDEKFNRHNLIGQSYPMVKLLETVA
                     QVAPTEANVLISGESGTGKELIANAIHFNSMRAETVFIKINCSALTETLLESELFGHE
                     KGAFTGAERRREGKFVQAHGGSLFLDEVSEMSKAMQVKLLRVLQERELTRVGGAEVIK
                     VDVRVIAASNKNLKEEVQNGNFREDLFYRLNVVSLNVPPLRERKEDIPILAQHFLELF
                     AGRNKKTIKAFTPRAMEKLLAYHWPGNIRELMNAVERSVVLSRTDYLDADDMTLVMEK
                     SHEVSGIAEQGEAIRNLSLESMEKKNILAVLADCRGNKSETARRLGITRKTLRAKLIK
                     YEMNR"
     gene            complement(18994..20226)
                     /locus_tag="PLR13_03155"
     CDS             complement(18994..20226)
                     /locus_tag="PLR13_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18360.1"
                     /translation="MNNFFIFIFVFVLSIAVIISFIYTMWRMNRDEQKLISIIHGSPI
                     PTFVISKNHKVVYWNRALQAASKIKPSDVLGTDQHWRAFYTSPRPCLADLILDGKTAQ
                     IAELYPDRWSRSKLLEDAYEVTQFYPDMGEKGKWYRITSAGLKDSRGNLFGVMETLED
                     ITEQKLAQDELLRRTKLESLGTFADGVAKDFDSLLTAILRNVFLAKISASDEDKMLEN
                     GLAVAERAGLQAKELAHRLITFAQGGYPVRKLEKIEPVLHEAAESVFKNSAITCNITI
                     AEDLWPCEIDATQIKQVIENVLINAREAMPDGGSVQMTTENTTTGTNIRSLGQGNYIR
                     IIIKDSGIGIPRENLPRIFDPYFTTKKTKEHGGIGLGLAISDSIIKYHNGLITVESIV
                     NSGTTFSIYLPAKTTNGS"
     gene            complement(20634..>21050)
                     /locus_tag="PLR13_03160"
     CDS             complement(20634..>21050)
                     /locus_tag="PLR13_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQC18361.1"
                     /translation="KTDYVNVDATCMWMVKNPEWFDVIVTDNMFGDIITDLGAMVQGG
                     MGIAAGGNINPEGVSMFEPIGGSAPKYTGKNIINPLAAICAGGMLLETLGEEKAAKRV
                     EAAVVKLLESGKLKSLDAGKMGMSTSEIGDWLSDNI"
     assembly_gap    21051..21150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21151..21485)
                     /locus_tag="PLR13_03165"
     CDS             complement(<21151..21485)
                     /locus_tag="PLR13_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQC18362.1"
                     /translation="MTSSKSYKIAVVPGDGTGPEVVAEGLKVLKAVAASESIRYELVH
                     YDLGGERYKKTGEILPDSVLEEFRKFDAIYLGAIGHPDVKPGILEKGILLRMRFELDQ
                     YINLRPVKL"
     gene            21824..22765
                     /locus_tag="PLR13_03170"
     CDS             21824..22765
                     /locus_tag="PLR13_03170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQC18363.1"
                     /translation="MGEGIGDYTKNIVDLRRHMWMAGVEVELRRLIWQIAVVGKYISA
                     KIHESNRKLAGLKNVYGEEQMALDRASDEILKNQLEFSGFVREYASEELENIVKIGTG
                     QEKYIVTADPLDGSSLVDTNLAIGTIIGIHKESVLDCGRNTMVAALYITYGPLITMIY
                     SAGKGAHEFVLNREGEYVLSQENIKLKEKGSIFSPGGLRRDWNPAHLQFIDKLENDGY
                     KLRYSGGFVPDINQVLIKKGGLFTYPALKKSPKGKLRLLFELQPMAFIMEQAGGLAID
                     GDQDILSVKVETINQLSPIYIGSTYEVQLAKEMLSAS"
     gene            22852..24243
                     /locus_tag="PLR13_03175"
     CDS             22852..24243
                     /locus_tag="PLR13_03175"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQC18364.1"
                     /translation="MIYKDKNELQKKYEKIIAIEGNDVSILNETALRGDLIDHLIYTA
                     VFSADQAAQQAACWLIRRAGAALGIVSASIQPLYEAMGREDVRGFTVPAINLRGLTYE
                     SAQAVFRAMMKGKVGPVIFEIARSEIDYTSQRPVEYTAAVIAAAIKTGYRGPVFLQGD
                     HFQINAKKFAADAAKETQTVKNLIWEAIEAGFYNIDIDTSTLVDLSKPTVTEQQQTNF
                     SLAAELTTMIRDLEPEGITISVGGEIGEVGGKNSTVEELEAYMDGYLAELKKQGESLK
                     GISKISVQTGTTHGGVPLADGSVAKVKIDFAVLEKLSAVARSKYGLAGAVQHGASTLP
                     DEAFDRFPAVGTAEIHLATGFQNIIYDSSAFPADLKARIYDHLKTAFQSEWKDKDTEE
                     QFIYKTRKKGFGPFKQDMWHLPADTRDKISAGLEKQFAFLFDKLKMNNTQDVVEKYVQ
                     PVDVAPDVPVSLK"
     gene            24240..25271
                     /locus_tag="PLR13_03180"
     CDS             24240..25271
                     /locus_tag="PLR13_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HQC18365.1"
                     /translation="MKAAILEQTTSLKKNKKPLKITEVAEPAVGDGDDVLVKVSVCGV
                     CHTELDEIEGRIFLPSFPVILGHQVVGVVAGQGRRARKFAIGDRVGIGWINRSCGQCP
                     FCLSGNENLCAGFKATGRDVSGGYAQYLTVSEKFAFPIPEIFSDSQAAPLLCAGAIGF
                     RSLRLANLTNGQRLGLTGFGASAHLVLKMARYLYPQTPLFVFARNPAERQFALDLGAV
                     WAGDTSDESPEKLHAVIDTTPVWKPVVEALKNLEPAGRLVINAIRKEDTDKDYLLQLN
                     YGRHLWMEKEIKSVANVALCDIENFLKLASDMRIVPDVQAYPLNDVNQALGELKEGKI
                     RGAKVLIIS"
     gene            25428..26993
                     /locus_tag="PLR13_03185"
     CDS             25428..26993
                     /locus_tag="PLR13_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQC18366.1"
                     /translation="MKIASVGEMRSMDRFAIEHLGIPEDVLMENAGRAAVSVLLSRTG
                     IRGKKFAIFCGSGNNGGDGLVVARLLHSRGGLVRVFLLNDSEKFRGAAATNFEIVKRL
                     PLETAQLTNARPAGRHVAHCDVVIDAILGTGLDRPVSGLVADTISLINKGGKKVLSLD
                     IPSGVNGNTGEIMGAAVTADYTVTFGLPKIGNLLYPGCDVNGELWVSHISFPPSLYDR
                     DDIPVRTNDAMALPPRPADAYKGAMGDVLFIAGAVNYYGAPYLSAMSFLKAGGGYARL
                     AAPRAVIPVIARRGREIVYLPQEETEAGSMALKNKKALLEHSAKADMVVIGPGLSLQD
                     ETVNLVREIAATVRKPMLIDGDGLTAVAGCPDSIRRRKAATILTPHMGEMARLTGKSL
                     KEIHADKIKVLRETVQTLNACIVLKGPHSLIGTRDGNVYVNLSGNAGMATAGSGDVLT
                     GCIAAMFGMGLSFDEATRKGVFLHGVAGDLAALKKGTDGMTAQDIMNALPQALKEDRS
                     GRLQDQYALPDGV"
     gene            27046..28389
                     /locus_tag="PLR13_03190"
     CDS             27046..28389
                     /locus_tag="PLR13_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQC18367.1"
                     /translation="MQINRLTLKFSGELSYLEAPFLKEYYTSSLPHIRMMMILGAMLY
                     AAFGVLDVLLMPQQVVALWIIRFIVIGPVLVGVLAISFLPIFEKYIQPILALVYFLAG
                     AGIIGMIVVAPPPVSYSYYAGLMLVFMWGYTLIRLFFTWASVAGWLQVIFYETVTVGL
                     NPTPLDVFLGNNFFFISANLVGMMACYAIEFYARRDFLMNQQVEEEREKVSRINEELE
                     DRVKQRTEDYKNLNQALLQEVAGHEQAKEKLQEALDSLKKSMGVTIQVMVSAVEARDP
                     YTAGHQIRSSNLACAIAAEMGLPQEKIDGIQMASSIHDIGKLSIPAEILTKPTKLTNL
                     EYSLIKEHSACGYEMLKNVESQWPLAEIIFQHHERMNGSGYPRQLKGDEILLEARILA
                     VSDVVESMASHRPYRPSLGIGAALEEIEKNRGILYDSAVVDACLKLFREKNFYLE"
     gene            28483..30264
                     /locus_tag="PLR13_03195"
     CDS             28483..30264
                     /locus_tag="PLR13_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18368.1"
                     /translation="MKKYYIMYDPTTVDLMGKIGVAGFTVEDTVEYLTGRKLEACNET
                     NYTEFLSFNTNLLRKGMLEQMAIEPYKAYYIANQICRADQDARVIIADDTRKTIGQII
                     AEEKQKPVAVFMSLISSTFPTACAVILILNKVNIPVVIGGIHVSTSFNDVDMYLRKYL
                     PHPQIVTQVIGAGDLPTIKNVMSDLARSNLKKEYHGNIPIEDGVWGCDRVIELPKIKP
                     YFINKFPVIGPLLSRIIETNVTTPFLGCPFSCSFCSISSFPEERRKFTSRSPEDFTCE
                     LLDKQKKGVTFKNRFYFISPDNLLFGGKKLHDVLDKMIESPLRINYAAQISIEVADSD
                     KLLQKLRLSGASHFFIGLESLDIRNLEYVGKNIVAKIKKEKISVEEYYASRIKKIQDH
                     GISVHGAFMFGMPYDYFHSLEDHSGRKIVEFCKKNKIGIQPTCLSNLPGSLDFLKGLE
                     THELIYGNPGSMDYFCSLTIADLTESNRKIPDALFNSPLVTFYMLYDTMEKIGSYISA
                     LHIAFGMAKKSWSVHSSKGAQSFKDRMVDAFAGIGFQLGASAYFELYKGLAYSTSSLP
                     GTFERLYQWEKNPTVRKMFKKYVENFV"
     gene            30278..30925
                     /locus_tag="PLR13_03200"
     CDS             30278..30925
                     /locus_tag="PLR13_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC18369.1"
                     /translation="MSKITRSEKEIEEVRNAILEEALTILREEGFEKLSMYQLGRRTN
                     MTAANLYNYFRNKDQLIIAIHKKTFELLYAMLSKAVRKEAEPMAKLKKIIKTFLTFAK
                     KYPNYYDIMFNRRVPQVSDYYGTSEESLAREEYESSARNLNLVMSIGKNVLSSSSEPD
                     VRDVEFYFIRTFSELHGIVSLYNSGILKVIVKNPERAMHRIVDDLLTAYIRRKYR"
     gene            31150..34224
                     /locus_tag="PLR13_03205"
     CDS             31150..34224
                     /locus_tag="PLR13_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQC18370.1"
                     /translation="MQNTLENSSDQNRRSGYWGLAVSASKVGIGKVLRKHQLLLEGFL
                     QFIAGRFNTSEALLEELKGTQGWINLCLGMRSEDSAIEAAIVFQDGVVRVLKTIPENV
                     ETVIIFKTSDHLLDMTDATPDEMYKMMLIGTIRTQGNIMLASLFNYLMALVFDKGQQK
                     AVDRQIEEHRKANKSVGRDVADTDCCRQERQRRKISRVAGGKVDPGVKYLEDPHLAGL
                     GLEDFPRLEQFRAEYFGKKKEAVVCHEYGKLITDFHLANGYEVDKDGKPWDPNLRKAE
                     SLKYILENRTPVIRTNDLLAGTYTTSPVSTCVGHPFSIGCYSWGELRSFSKRELMPYE
                     ISEESIHILHRHVFPYWAKRNIHELWRSRTNGGLPVQIHDRFFSVYYWKTISMSEVPP
                     GHEALIKLGTGGLIRKIEEELARDAHADDEKKNTLKAMIISLEGVNAYARNLARQALE
                     ESKTATNPQRKAELETMHRMLLKIPESPSETLHEAVQNIILMHLCLGMESTDDGPMYG
                     RLDQILQPYFESDMHKLTTPQKREAYIKQVIDILGCLYFIESSHQILAPDIGNWQNGG
                     SSPNGTITLGGVTPQGEDAVNDMTYILLKVTELLSLNNPNVHARYKPDKNSFAYLKRV
                     CDVNYITGATPCIHGDDAVMESLTARGWAVEDVRDWVVNGCVEPGIPGKHCSATSSIE
                     FNLVAVLEMALNNGKHPLMNWKLGPDTGIIGQGDFETFDDFWKAFKEQCEFLCEQSII
                     GNNQLGEIYQQHQPAPLISSMTEGCIESGRGVTRGGARYNSSGVSVIGMADVVDSLMA
                     IKTLVYDEKKITFAELKEAIDSNFAHHPRLHVMIKTRVPRFGSGNEESVTMANRVTSL
                     VHDYFASHQNYRGGPHATGWWSMANHTVYGRVTGASPSGRLAGEPFTPGLTPHPTASP
                     NLLDNLRDVAKLKPETLDNNIAFNVKIVPASSDSHERIIEIMANYADTYFRMGGMQTQ
                     FNVISTETLKDAMAHPEYYSDLMVRISGFVAYFTRLQRDLQLEVIRRAEYKI"
     gene            complement(34228..35202)
                     /locus_tag="PLR13_03210"
     CDS             complement(34228..35202)
                     /locus_tag="PLR13_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQC18371.1"
                     /translation="MQIQEIFSIYKEDLQQVEKCISDNAISEIKLIPEVAHHLIDSGG
                     KRFRPLLLLIASRLCGYHGEHRYAMGTVMEYIHTATLLHDDVIDQALIRRGKTSANKV
                     FGNSVSILVGDFLCFKAFMLMSEIGNNNIFELVSRMGNIMSEGEVFQLTKCGDINMTR
                     DDYLNIIEKKTAVLISASCAAGAILGSASPEKIDAMARFGKNAGMAFQITDDILDYAA
                     KGQTFGKSIGKDLEEGKITLPLIFALKQSTDEEKNTMKEILSRKKSSRKAALQVRTFI
                     EKYEGLELSRYTAAGYIKEAKRQLDVFEDCAEKKHLITVADYILSRNV"
     gene            complement(35225..36862)
                     /locus_tag="PLR13_03215"
     CDS             complement(35225..36862)
                     /locus_tag="PLR13_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQC18372.1"
                     /translation="MTTSPDRKETEQTGVSFNTKQTETTSVRPELAEMFARQKFGLDQ
                     NRPEAVEKRRQKNQRTARANVEDLCDAGSFMEYGALAMAAQRGRRSEEDLMARTPADG
                     LIAGIGTINGTFFKEEKARCMVMAYDYTVLAGTQGFFNHKKMDRMLKLAHEQSVPLVL
                     FAEGGGGRPGDVDAIGVMTAGLDLSTFAMFAGLSGKVPLVGIVSGPCFAGNAALLGCC
                     DVIIAAKNSNIGMGGPVMIEGGGLGNFKPEQIGPIDVQTQNGVVDIEVADDVEAVAAA
                     KKYLSYFQGTTPSWEAADPLQLRDLIPENNRRAYNMRTVIKALADTDSYLELRPKFGA
                     GMITGLIRIEGRPFGLIANNCLHMAGAIEAEGADKTVRLMQLCQAHGLPILSLCDTPG
                     FMVGPEIEKRAQVRHVCRMFVVGSHLTVPYFTVVLRRGYGLGAMAMAKGGFHESFFTA
                     SWPTGEFGAMGIEGAIKAGFKKELAAIEDPDQREQLYRDLVAQLYKRGKAVNIASYLE
                     IDAVIDPSETRKWIMRGIKSAATGKKQSPVHNYVDPW"
     gene            37044..37433
                     /locus_tag="PLR13_03220"
     CDS             37044..37433
                     /locus_tag="PLR13_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQC18373.1"
                     /translation="MTANLLKIFNPVKRNKTVLMKKSLSCLQCGKCCFVDFTAYAQQE
                     DYDRWRAENRQDILEMIDHRHLFWAGDRMISSDTGNAPGECPFLYNTGKVWLCSIYET
                     RPLICRDYQPGSSELCPQWKNRKKKEE"
     gene            complement(37444..38802)
                     /gene="rimO"
                     /locus_tag="PLR13_03225"
     CDS             complement(37444..38802)
                     /gene="rimO"
                     /locus_tag="PLR13_03225"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQC18374.1"
                     /translation="MKNAEKTKINIISLGCPKNLIDSEVMGGFLRQSGHALVDKHTDA
                     DIVIINTCAFINPAKEEAIDEILTVAEAKKNRPNLRIIVAGCLAQRYGRELLDQIPEA
                     DRVIGTGEVGNIVRHVTELIRCKNRRKAVITRPVYLMNCNHRRILPASAFSTYLKISE
                     GCSNRCSYCVIPSLRGAARSRPPDDILREAERLAQRGIREIIITAQDTTGYGWNLKGR
                     PRLSDLLNDLTQIKGIEWIRLLYAHPARITADLLSTIAENDKICSYIDLPIQHIDDAI
                     LNAMKRKVNAAEIRQVIAQIRHIIPDVALRTSLITGFPGETPARFNRMLDFVRQTRFD
                     HLGVFTYSREEGTEAAGLRYHVPEKEKERRRETIMHDQMEISASINTSLIGSVQEVII
                     EGKSDRPDYPYIGRCRRQAPEIDGVTYIKGPSPDIGHIVKCRITAADDYDLFGKIIRK
                     SS"
     gene            complement(38799..41054)
                     /locus_tag="PLR13_03230"
     CDS             complement(38799..41054)
                     /locus_tag="PLR13_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQC18375.1"
                     /translation="MEKKQTDNKRTREIKGAVCLALGLFLLLCLVSYHPQDPSFTHFV
                     AGDHTTHNFTGKAGSYTADSLIRLLGAASLLFPLMLFVCSFKYFRSSDFRIGTGRLIG
                     FLFFIFSFAGIMALLISGSISLYGESLKNGTGGLTGVAIVGLLRNYFNVAGTYIILFL
                     IFVVSLTFLVDFSMISFTEKISKSGAAFLLRWKERIMAFVDFVFNRMKSEPPSPPQPA
                     IVEKAPDVTKKAPAKKVEQTHFDFTKVKSDDKFQLPPFTLLEEAPQKDARVKRDALVT
                     NSRILEKKLSDFGVEGRVVEVTPGPVITMYELEPAPGVKINKIVNLADDLALALMAPS
                     IRILAPIPGKSVIGIEIPNLKRESVFLKEVIDNDSFTQSSFRLPIALGVNFVGTPVIA
                     DLTKMPHLLIAGTTGSGKSVALNAMICSILFKAQPEDVKFLMIDPKRLELSSYEGISH
                     LMHPVVTDPKKASQVLRWTVEEMERRYKVLNDLKAKSIDAYNELLLKGLKSKTVLAMP
                     KQIDPELESGQTIDETAREEQEIKHTKLPYIIVVIDELADLMMVAPRDVEESLTRLAQ
                     MARAAGIHLIIATQRPSVDVITGLIKANFPTRISFQVSSKIDSRTIIDQQGAEKLLGS
                     GDMLFIPPGTSKLSRIHGAYVSDKEISRIVDFIKMQAQPAYDDSIEKYALAAAQIQHE
                     GDDDFDAKYDEAVALVSELGQASISLVQRYMKIGYNRAARIIEKMESEGIVGPSDGAK
                     PRKVLIRKLPG"
     gene            complement(41093..>41809)
                     /locus_tag="PLR13_03235"
     CDS             complement(41093..>41809)
                     /locus_tag="PLR13_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQC18376.1"
                     /translation="SSERSGKRGTLNLLTVIVPGIVQEFTELFPVSRFEHLVIVQSFF
                     PGFHQPGGAFDAVLLLIIAAIPVAVIGYLFKDQMHNIFESARAAAFFLIITGILLFLA
                     DKMKKTRRDIKNMTVIDGILSGSARAVPFLTGISRSGTTITSGIFCNLNRNAAARFSF
                     LLSLPAVCGAVILKAGYLRQISSPGSYLYFAGFVCAALAGLLALKLFFLIIREARLKY
                     FAYYGWFFGTFTLLVKSSLF"
     assembly_gap    41810..41909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41931..42683)
                     /locus_tag="PLR13_03240"
     CDS             complement(41931..42683)
                     /locus_tag="PLR13_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18377.1"
                     /translation="MVHSALLVTLGVAITAFMSFWSVVFSVFPAADNKIHKVATLWAK
                     LLLMICNTKVDVIGKENILFGKPQIFMANHQSDFDILISLAHIPVQFRWIAKKELFAI
                     PVFGAAMRSAGYIEIDRSNREKAMQSIDEAALRIRSGKSVMTFPEGTRSREGEIKAFK
                     QGAFHLAVKSGVPIIPISIIGSGRIMPKRSLRIKPGQLKMVIGKPIDVQDVTIENRHA
                     LIEKVRNEIIKNYNAWQDIEMSPLKDLESGAH"
     gene            complement(42823..44655)
                     /locus_tag="PLR13_03245"
     CDS             complement(42823..44655)
                     /locus_tag="PLR13_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18378.1"
                     /translation="MQEIKTANAKKTPPVKSRKDTKEAFLYRPQPEEKHENWLQKSLF
                     LFQHNPASIVLLRLPEKKYIDINKTVLRTFGFSRQDVIGKTSDELDIFVEQDKRQIAL
                     KQLMQQGYIENFEMKFRRRDKTILDGLFSAEIIQHREKRYVLVVITDITSRNLAQQAQ
                     RENEEKYRTILETTEEGYYEIDLAGNFTFFNDPVCALLGYSENELRGMNNRQYTSRET
                     ARKVFQAFNKVYRTGRPTKEFDWEIIRKDGTKRYIEQSASLKKDSSGKPIGFRGIIHD
                     ITERKQMEDALRKSEENFRHSLDDSPLGVRIVTEEGETLYANQAILDIYGYTYVDELK
                     NTPLHKRYTAESYADFQQRQEKRLMGEAGPPEYEISIVRKDGEVRHVLALRKEIFWNG
                     QKRYQIIYQDITERRKAELKLQETLHTLRKSINTTIQVLGIASEARDPYTAGHQRRVA
                     DLARAIATEMKLPQDTIEGIRMAGAIHDIGKISIPSEILCKPTTLSELEFSLIKSHCQ
                     YSYEIIKDVESPWPLADIVYQHHERMDGSGYPRSLKGEDILIEARIIAVADVVEAIIS
                     YRPYRPALGIEIALAEIENNAGTLYDVEAVSACLKLFRNKNFKI"
     gene            complement(44790..45494)
                     /locus_tag="PLR13_03250"
     CDS             complement(44790..45494)
                     /locus_tag="PLR13_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18379.1"
                     /translation="MLNIKQLRYGTDNFSYLLYGKTQAVAVDGGAWKEILDFLKQNNL
                     MLKIVTNTHSHYDHTPGNDHLLQKTGAEFIRAADFQDHQPLVINAERILVYRTPGHTD
                     DSVCFHAGSFLITGDTLFNGTVGNCFSGNMNIFFLSIKRLMDLSGETMIYAGHDYILN
                     ALAFARHLEPRNKNIDLFRNACRSRNTTYSTLDEERGINPYLRFNEESIIHLLRKNNL
                     PHATEQERWNSLMSLE"
     gene            45738..>46296
                     /locus_tag="PLR13_03255"
     CDS             45738..>46296
                     /locus_tag="PLR13_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQC18380.1"
                     /translation="MQKLLTKKRALEIIAGFPRAGVLVIGDVMVDHFIWGNVSRISPE
                     APVPVVDVQKDNILLGGCTNVLNNIHSMGGRAYVSGVVGADTIGKQLLAELNDRKIET
                     KGIVVKKDRPTTLKTRIVAHGQQMVRFDKEDRKPISPVSIHKILDYVRSRVDKIGAIV
                     ISDYNKGVVSKELIQGIKKIVDHSGI"
     assembly_gap    46297..46396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46480..47013
                     /locus_tag="PLR13_03260"
     CDS             46480..47013
                     /locus_tag="PLR13_03260"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HQC18381.1"
                     /translation="MSKPSPAEPVKLFFSLFAQDAVIIDETIKELSRAYGDPDFISAL
                     QPFDYTDYYGGEMGENLIRRYLTMEKLIHPESLPDIKLATNDIEIKSAAGLKRRINID
                     PGYISKAHLILATGKSYTHRPYLRDGIYADLTLVYQGKKFCVLPWTYPDYADAKQLEM
                     LDRIRNRYLTQLKMTDH"
     gene            47054..47932
                     /locus_tag="PLR13_03265"
     CDS             47054..47932
                     /locus_tag="PLR13_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQC18382.1"
                     /translation="MIKSMTGYGRAEKVFQDKNIIVEAKSVNHRFLEIVLRTPSALFP
                     MEMDYKKKITERFKRGRIEVFIKFEGEGADTAKVNLNMEIARGYFNVLNRLREEFDLE
                     SPVTLKTLTAFRDIFTPPAETEIGSDFLCEVEKSFLEALEILAAMRQGEGLVLLQDMQ
                     MRLKMIDGVLNDIAVRAPQVVSEYQKRLSERIKELTEGFALDENRLAQEVAIMAERTD
                     ITEEIVRLRSHLGQFEVLLHSDESEGRKIDFLLQEMNREINTIGSKVGDVDITRHVID
                     VKSELGKLREQAQNIE"
     gene            47937..48539
                     /gene="gmk"
                     /locus_tag="PLR13_03270"
     CDS             47937..48539
                     /gene="gmk"
                     /locus_tag="PLR13_03270"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQC18383.1"
                     /translation="MSKGLFIVVSAPSGTGKSSICQGFMEAFPEIKFSVSYTSRPPRP
                     GEIDGKDYNFISREAFRKKIRQGEFIEWVENYGHLYGSSRRFIEEGVRDGRDLLLDIE
                     PRGAKKIKQEFRRGIYVFILPPSRQELLKRLKKRGHERENVIQKRYKQSESEIKEISW
                     YDYVIFNNDLKKAVNELIAIYTAEKCKRTRLNREIREFIK"
     gene            48568..48837
                     /gene="rpoZ"
                     /locus_tag="PLR13_03275"
     CDS             48568..48837
                     /gene="rpoZ"
                     /locus_tag="PLR13_03275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQC18384.1"
                     /translation="MARITIEDSLRVAKTRFGLVSLASKRAHQLLKSSQPLVVSKNRE
                     IVQALREIAAGKVVYANPEYLNGTKEDFKPIPDSTEFIGDEEYIE"
     gene            48818..49570
                     /gene="pyrF"
                     /locus_tag="PLR13_03280"
     CDS             48818..49570
                     /gene="pyrF"
                     /locus_tag="PLR13_03280"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQC18385.1"
                     /translation="MKNISSKNAGERLIFALDVQNYEDALSLVDLLSGHVGMFKIGKE
                     LFTAAGPKIIRAIKERNQKVFLDLKFHDIPNTVARAAEAAVKMHVDMFNVHASGGSRM
                     IRESVSAAWTCSDALGRARPILLAVTVLTSLNNDDLKEIGFQKTTNELVVHLAGLAKA
                     AGASGVVASAQDIVMLRKAFGEDFVIVTPGIRSAAEIRKDDDQKRTLSAYDAVAAGAD
                     YIVVGRPIRTAADPLKACRQIVQEIAEGLSAR"
     gene            49596..50351
                     /gene="rlmB"
                     /locus_tag="PLR13_03285"
     CDS             49596..50351
                     /gene="rlmB"
                     /locus_tag="PLR13_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQC18386.1"
                     /translation="MEFIYGINSIRDLLRLQKSGWEKIMIASGRTGPAVQDIIAMAEA
                     HNIPVEFCERKKLDKLAGTPNNQGMIAFRQTFAYADLETVMKNRHPACGHDLILILDS
                     VMDPQNLGSIIRTAHCWGANGVIIPEDRSAGVTAAVNKASAGSVHHIPVAKVVNLAQL
                     LDHLKDRGFWIFGAETHGGKKLTETDFDCPVALVMGGEAKGIRPLLKKKCDFLTTIPL
                     TGNFDSLNVSVAAGIILYEISRQRTRSTKQSGK"
     gene            50522..51730
                     /locus_tag="PLR13_03290"
     CDS             50522..51730
                     /locus_tag="PLR13_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQC18387.1"
                     /translation="MPVFIWEAVTKKDEAKKGEMEAVDEITVRSLLRRQGYKSIEVKR
                     KPKDLLEYLPFLQGKVKEKNVVIFCRIFSTMINAGLPLIQCLDLLAQQEQNKAFSKII
                     KSIKEDIEGGTSLTNALKKYPEIFDELFVNLIAAGEAGGILDIILQRLSSYMEKAMKL
                     KARVKGAMTYPAAVLVISCAVVALLLIKVIPVFQKMFEGMGGELPALTAALIAASQFA
                     QSYWYLGVAILVVIYIILSRFYRTEKGRWTIDSLILKSPVFGPLLKKVAVAKFARTLS
                     TMMSSGVPILEGLSIVSKTSGNVVIEDALMKTRQGISEGQSIAEPLTKTGIFPPMVVQ
                     MIAVGEATGALDSMLSKIADFYDDEVDAAVDAMTALLEPVMMVFLGGVVGGMIVAMYL
                     PIFKLASVVG"
BASE COUNT        13250 a        11928 c        13576 g        13010 t
ORIGIN      
        1 ccacggacat gctggttaac agccagccgc tctaccgact gagctactgg ggaacgtatt
       61 ttcaaagatt ccgaattttt gccccgggta ttgatccgac atgacggatt gccgaagcat
      121 ttttttctgg cgcttcataa tacaagaaga gacagatgtc aacatcaatt aaatatttat
      181 tcaaaatgat aaaaaaccgg atctttatat gaaaaatgcc gaattctttt gcaatgttcg
      241 gaaaattgac cgccgaaatg tttcatcagg ccgtatcatt tccttgcctc aaaaatcacg
      301 ataggatata aaagcctact tttatgaatc agaacggcaa ataaggttgg caatgatgac
      361 aggcagccag aaattcttga aaaaacatgt tttggacgag gggttatgca ccggttgcgg
      421 cgcctgcgtg ggcatttgtc cgtatcaggt gatttatcat gaccgaacgg tgcaattaca
      481 cggctgcgat ttggtaaaag gccgttgtta tgattattgt ccccggacgc cctgtgatgt
      541 cggagccatc agggaacgat attgcgcagc cggtgatctg acgccggaaa tcggcgcggt
      601 caagggatat tatttttcca gagctgctga tcctgggtta cgaaacatcg gccagcatgg
      661 agcaacagta acggctatgc tggagttggc cctgtctgaa ggcctgattg atgcggctgt
      721 tgtttcttcc cgggaccaat tatcgctgcg aaaaggcgtt attgtacaaa acagggaagc
      781 gatcagaaca catgccggca gtcaatttac cgtatcggcg acggtagccg cttttcacca
      841 actggtgacg aagcaaaccg gtcaagtcgg aattgtcgcc acgccgtgtc aggcgctggc
      901 cctggccaag atgaaattca atccgggaca tgaagacatt gccagagtca atcaactgac
      961 gctggtaatc ggtttgtact gcggctggac gctttccgaa gaaaaatatt taaagttact
     1021 gacggataaa ggcatcgctt tggaatccat aaccgaaatg gacatccccg ccggcaggaa
     1081 cgttctggag atccggacgg aagatcggct gcattcgatt gcgatggagg aagcgcaaac
     1141 ctgtatccgg gaagcttgtc tgtattgtat ggacagcacg gcggaattcg ccgatgtgtc
     1201 ggtcggttcg gcgcggtttt cgggaaattg ggaagacgtt cgccagtgga atcagttgat
     1261 cgtcagaacg acaaaaggga aagaactgac ggatctggcc gttgagaagg gcgttctgga
     1321 attgcgggag gcgcccgccg acagtttaaa agaactgaag gaagcggcgg tcaataagaa
     1381 gaaaaatgcg ttgaaaaata ttatccaaaa gagcggctcg gcaaaaaatc tgttatactt
     1441 ggacagtcgc gatccggcgg tgctgaaata tttggaagct gaaaagaaaa aaagtaaaaa
     1501 gtgaatggtg agaagtgaat atttgtgcct ctgttcagcc aagtcttttt gacttggctg
     1561 tcaataaaaa aatcagtcgg gtccaaaaga ctcgactgag cttgaggggg gggaacggcg
     1621 gggtaaacaa gagaaaatca atgaaatgtc aagaataatg aaatgatatt acaccctcgg
     1681 catgacaact tttatgaaat atattcataa gattttacgt ttgattttat tttctgtttc
     1741 catatttatg tttgcctcga gctctgcttt ttgctccgtg gcttcagggc ggttcagtat
     1801 cggtaatatg agtgaggaca ttgcgatatt aatagcctat gttcttctgg ctttattggt
     1861 ttcatttcta tgttcaatag cggaagcggt cttattaagt attacgcctt cgttcattga
     1921 ggaacagaaa gaaaaaaatc ccaagcgggc cattctgttg aagcgactga aacaggataa
     1981 tgtggatcgc gcgctggcgg cgattttaac actgaacacc atcgcgcata cggtgggcgc
     2041 catcggggcg ggagcaaaag ctacggtcgt gtttggaagc gcatggtttg gaatcttttc
     2101 agcggttatg acattaatga tcctttttct ttcagaaatc gttcctaaaa ccattggcgc
     2161 catttactgg cccaaactcg ttgtgcccac ggtattttac gtacaaaccc tcattgtaac
     2221 gctgtttccg attgtttgga tttcagaaaa aatgacaaaa tttatttcgc acggtaagaa
     2281 tctgcacatt tttagcagag acgagtttgt tgccatggca agagtgggcg aacaatccgg
     2341 acagatagac gataacgagg cgcggattat caagaatctc ttccggttcg ggtcgctcaa
     2401 cgtaacggat atcatgactc cacgcacggt gatcgcggca ctttccgagg ataaaacggt
     2461 agccgacgcg gcggaatatg ttgtaaaaat accattttca cgaattgtga tatataagaa
     2521 ggatatagac gatataaccg ggtttgtttt gagagatgat gtgctgctgc gggataatca
     2581 ggaaagaggt ggcgaggcgc tcaagacctt aaagagagaa atattggttg ttcccgagtc
     2641 tgcagtactt acggatttac tggaaatttt tctaaaagaa cgtcagcaca tcgccattat
     2701 agtcgatgaa tacggaggaa caacagggct tgttacgctc gaagatctga tcgaaacatt
     2761 aatgggtttg gaaattgttg atgaaatgga cacggttgtg gatatgcgtg ttttggccag
     2821 aaaaaaatgg gaagaacggg caggggcgct cggcataaaa attaacgtgg atgggaaaga
     2881 tcaatccaaa aaagaaaatg tgctaccgga ataataaaga agccgactct gtataataag
     2941 aaactaaact tccgcgcagc gtcggaaatg atagaaatac ttttaagtga attataaaat
     3001 gataaataaa ttattatcac cggccaaaac agaagtgttg ttcatgggca acgaagccat
     3061 cgcgcgcggg gctctggagg ccggcgtaag tgtcgtttcc ggttatcccg gcacgccgtc
     3121 ttcggaagtg atcgagcgcc tgtccgaagt ggcccgtgaa agaaatctct acgtggagtg
     3181 gtccaccaat gaaaaggtgg cgctggaagt ggccgccgcc gcatcgtttt ccggtttgcg
     3241 ttccatgtgc gtgatgaagc aaaacggcgt caacgttgcc tccgattttc ttctgcatct
     3301 ggcctcgtcg ggaacacgcg gcggcatcgt gctggtcgaa tgtgaagacc ccggcgcact
     3361 ctccagtgtc aacgagggtg aatcgcgcta tttcgcgcgc atgctcgaaa ttcctatgct
     3421 ggagcccgcc aattttcagg aagccaagga cctgacccgg tgggcctttg agttatccga
     3481 gaaaatccga aatatcgtca tggtgcgctc ggttacgcgt atgtcgcacg ccagcggtaa
     3541 tgtcatctgc ggcagtctgc cggctaaatt ccgcaacgcc cgctttattt ccgacggatc
     3601 ggtcatggat cctctgcgcg ggccggtcgt cagcacgccg gtggttatta aacacggcct
     3661 gcaacagaaa aaattacaga cagcagcgga gctttttgaa aagagtcctt tcaacagcta
     3721 cgttggtcct aaaaaaccgg agcttttgat catcaccagc agcgcctgct tcctatacag
     3781 ccgggaggct cttgaaatac tgggcttgca aaaacgtgtc ggaattttga aattgagctg
     3841 tacctggccg ttgccggaaa agcttttgaa aaaatatctg cgcctgacca aaaagattat
     3901 gattgtggaa gaggtactgc cgtttctgga ggaaaacatc aaagtagccg cgatgaccat
     3961 gctgaaagat ataggcatca aaacgtttta cgggaaaacg gacaagaccc tgccttcagc
     4021 cggcgaattg aatcccgaaa tagttatgcg ggctctgagc aaaatcatga aggttaaacc
     4081 caagtccttg cctgccggtt atctcaagga agttcaaaaa gtggcgccca agattgctcc
     4141 gcgggcgcaa acgttctgtc ccggctgtcc gcaccgtgcg tctttctgga acatcaacac
     4201 ggcgctgaaa ctcgacggcc gcgaaggcat tgtctgcggg gatatcggct gctatgcgat
     4261 ggcctcgctc tggccggaaa tcggtttcca taccgtgcgc acgctgcatt ccatggggtc
     4321 gggcacgggg ctcgccagcg gctttgcgaa gctttcggct ttcggtctgg acaagcccgt
     4381 tatggcggtt tgcggcgatt ccacgtttta tcatgccgtt attccggcgc tgatcaacgc
     4441 ccaacataac caggccgata tcacctttat cgtgctggac aacgcgggaa cggcgatgac
     4501 cgggttccag ccacatcccg gtattaatga aagcgcgata ggcgaaccac tgcctcccgt
     4561 ggatattgaa gcggtctgcc gcgcaatagg cgcaaaggtt aaagtgagcg atccgtttga
     4621 tctggaagga acaagcagaa tactgacatc gttcatggcc gagcgcggct ccatgaaagt
     4681 gttaatcatg aaacaggctt gcgctttgag ccctgaaaag aagggcaaga aacagtacca
     4741 gatgaaagtc aatgaagatc tttgcctggg cgatcaatgc ggctgcaacc gtttgtgcac
     4801 tagaattttc cgctgtccgg cgctgacctg ggaccggaaa aagcaaaaag ccgtgattga
     4861 cgacgtcatc tgcgccggct gcggcgtctg ctactccatc tgcccgcaaa aggcgattgc
     4921 cagagtggag gctgaagcat gaaaaagtct cttaagaaaa atccaacaaa cattattatc
     4981 accggtgtcg gcgggcaggg aaacgtcatg gcctcgcgcg tgctggccgg aatgctggtg
     5041 aacgcgggct ttatcgtcac catcggtgaa accttcggca tgtcccagcg cggcggctcg
     5101 gtaatgagcc atctgcgcgt gtcgtcatcg tcggttctga gtccgcagat tccgcagggc
     5161 aggggggaca ttgtgattgc gctggagccg gtggaggcgc tcagagtact caccaaatat
     5221 ggtaatcccg atgtagcggt gctgaccaat tcacgcatgg tttatcccat gggcgtgatc
     5281 accggcgaat ttacttatcc gacgattgaa gaagtcaaat cgatgtttga aaaaatatcg
     5341 gcaaaaaact ggatcatcga cgccacgtcc gttgcggtgg aattgggaaa tccggttttg
     5401 agcaatatcg tgatgatcgg cgcgctggca ggtacgtcac tgttgccgat tgaccgccgg
     5461 gcgtttgaaa aggaaatcgc caaaaccatt ccagccggta aacatcaaat caatctgaaa
     5521 gcttttgatg ccggggtaaa aatgctctaa cgtaatgtag ggctgtagaa ggggtgcaag
     5581 gatggacgct gaccacggtt cttcaggcaa agtaacatac aattacaggt ttatattcaa
     5641 agacggcgtg gaaaaagaat ttaacatcgc gttggacagc gtcactttga atttactgcc
     5701 gacggataaa aaaaattatc cggaatggac aaagctgaat tgtttcaggt gttctcattg
     5761 tacgttaaac gatcaatgcg aatattgtcc catagccatc aatatggtgg atgtcgtaga
     5821 ttatttccgc aattattttt cttacgatat cgtggagaca attgttacgg cacaggaaag
     5881 aaaatatttt cgtacggtgt ctttgcagca cggcgtcagt tctttgatcg gaatatatat
     5941 ggttaccagc ggatgcccgg tgatggaaac attaaagccc atggtgcgtt ttcacctgcc
     6001 gtttgccaca atagaggaaa ccaaatatcg ggcgatttca atgtatttga tggctcagta
     6061 ttttctgtat cagcatggac gtcagcccga ctgggattta aagaaactgg cggaagcgta
     6121 tgagaatgtg cgaatcgtta acgaatactt ttgcaaaagg ctcagaatga tccaggggca
     6181 ggacgccagt ctgaatgctg tcgtgctgct ggatattttc gccgacagcg tcaatttctc
     6241 gatggacgga cggatggcca atgatcttga gtatttgttt aaaggatatt ttgaatcgac
     6301 gccgggtgcc aataacgctt cctgaattac cttgaattgt ctacaaaatt ttaaaagcgt
     6361 ttccgctcta aaaacaaccg ttcagggttt gtttttttca ggattcgccg ggtgacttct
     6421 tttgcgtata atcgtaaaat cccttgcctg ttttcattcc cagctcgccc cggtccacgt
     6481 atttcttcag caggtcggcg cttaattttg cctggtgatt attgcgtttt ttcgcccaga
     6541 aatcggtgat tcgccatacg gtttccagac ccacgtaatc catcatctca aagggaccca
     6601 gcggtgaagc ggtgatttcc ttccaggaac ggtccacgtc ttcgggagac gcgatgtccc
     6661 gcgaagccag attcaacgcc gccaaaaacc agggattgag aagcgagttg aacacgtacc
     6721 cggctttttc ctgcttaagg acaatcggcg aatgtccgat taattcgctg aaacgctgga
     6781 cggtttcaac cgtttccggc gatgtgcccg gatgtcccat cacatccacc agcttaaaac
     6841 cgggatgaaa atgccaggcg agaaattttt cgggccggcc ggttttatcg gcaaacatcg
     6901 acggcaccag agaagaggcg tttgtggtaa agatggtccg cgccgggcag accttgttga
     6961 gttctgcaaa aaaatctccc ttgatgtcgg gatcttcagg aatcgattcg ctgaccacat
     7021 cggcatcggc cccggcggca gcaagatcgg ttgtcgccgt gatgttgttc ttaaacgatt
     7081 cggccttgtc cttcggcatc atcccgaagt ccgccatttt atcaatcttt ttgttcatga
     7141 tgtccctggc ctttgccggg atgtcatcgt tgatgtcgta caggacgatg gcatagccgc
     7201 ttgtgacgca ggaaagaccg atgctccggc ccatggttcc cgctccggca atgagtattt
     7261 ttttaatatc ttcagctttc atttttcccc gctttccgaa atagctgcaa aaaaaaatct
     7321 atggtcggag tcggatgacc gattttcaga atgaaccaaa ccaaacattt atctgcttta
     7381 tttcgtgtaa aaccgtttta tttatccggc gcaaaataat gctttttacc ggtcaagtca
     7441 acagatcgtt tatgaaaatt ccagaaatcc cggacaataa tatttaaaag gggctgcttt
     7501 caatagtgtt aatttcccga ataatattgt atgccttcag tggctgtgat caaaaaacgg
     7561 gtgaactata tatgcgcata tgatagaaga aatccaaaca ggtccccatg tcagataaga
     7621 taaacatcgg tccatccgga cgtaatatta ttttctgtat ggttttgacg gcgttgattc
     7681 ttgctgctta cctgcaggtg catcaatttg atttcgtcaa tattgatgac agtgtctacg
     7741 tgactgaaaa cgattacatc cggtccggcc tgacgcttga tggaatccgc tgggccttta
     7801 ccaccttgta cgccgaattc tggcatcccc tgacgtggct ttcgctgatg ctcgattatg
     7861 aattatacgg cttacatgca ggcggctatc atctcacgaa tctttttctt catatcatca
     7921 gctccttgct gctgtttggg atgtttcagc ggatgaccgg cgctttctgg ccgagcgcct
     7981 ttgtcgccgc ttttttctct ttgcatccgc tgcgggtgga atccgttgcc tgggtagccg
     8041 aacgtaaaga tgtattaagc gtttttttct ggatgctgac gttatatttt tatgctctgt
     8101 acacggaaaa accggtattc agaagatatc tggcggccgt cttctttttt gcctgctcac
     8161 tgatgagcaa accgctgacg gtgaccctgc cggtcatcat gatgcttctg gattactggc
     8221 cgctgaaacg ttttgcatca cgcagacaaa atctgttttt atggcagttg aaagagaaac
     8281 tgcctttctt ccttctttcg gcggttttct cccttatcgc catttacgcc cagccgaaat
     8341 ttcctatcga cggttggccg ttttcgctgg aatcaagaat catcaacgcg attatttcat
     8401 tcgttcttta tctgcagaaa ttgttctggc cgtatgatct ggccatttgc tatccgtttt
     8461 tcggccgggc tcccgccgga caaatcattg cggcggtttt actgtttttc gccgtcagcg
     8521 cggccgtcgt ttggatgatg aaaaaatcac cgtgtctttt ggtcgggtgg ctctggttta
     8581 tcatcaccat acttccagtc atcggcatta ttcccgcggg aaacaatgcg atggcggacc
     8641 gttatctcta tctgccgtcc atcggcattg cagtcatgat ttttttcggg attcctctgc
     8701 tggttgaaaa tgaaaagatc aaaaagtttt ttttgccgcc cgccgctttg attttttttg
     8761 ctgttctgtc cgggatgacc tggcaccagt gcggtttctg gaaaaacagt gaagcactgt
     8821 tcagaaacgc ctcgcggatc aatgaggaaa acgccctggc gcatatcaat tacggcaatg
     8881 cgctggccgg tgagggcagg cttgaagagg cggttgtcta ttacaacaaa gcctggggaa
     8941 tgcttcctga gacaggcggg aacgtcatgg cgtataataa aatgggtatt gcggaaaata
     9001 aactccgcca gcttattgat gtcagtctgg gcaacgtcta tacaaagcag aaaaaatatc
     9061 aggaggctgt gcatcactat accagggcga ttgccatgcg tcccataacg ccggatcatg
     9121 tgcggaccta taatttcagg gggacggctt acagccgcct gggacggaag aaagacgcca
     9181 tcagagattt taatgaagcc atccgtctgg accgacgtga ttacagggct tacaacaaca
     9241 gaggattact gtacgctgaa acaggtatgc accggcaggc gctgcaggat ttcaatcagg
     9301 ccgttttgat gaacccgaat aacgcggagc tgtataagaa cagagggatt gcctgtgcga
     9361 gcctcaaact ttatcagaat gcttttgaag attttgaaaa agcactgaca ctggtccctg
     9421 atgatgccaa tatttattat aacagaggcg ttgcttacat cgaactgggc cgtcatcagg
     9481 aggctttctc ggattttaat gaagccattc gactgaaagc ggattatacg aatgcctacc
     9541 attaccgagg cggaatttac ctgatgtatg gttatgctgc atcaggatgt gatgatgccc
     9601 gcaaggcctg ccaactgggc aattgcgaaa tactggaggc agcaggaagc aaaaacttat
     9661 gccggtgatt ggttccggtg ggtcttcgtt ctgtaaaaaa tcttttctta aaaagccggt
     9721 cagagggatg aatgacggct gtctcacatc ttcagaaaca ggcgccatcc cgcataaagc
     9781 gaacccggaa tgaaatccag cgccccggac gcctcagcaa cgggcgtgaa tccgcaggca
     9841 tcgcagttaa tgttgccgcg gtttttgacg tagcagcctt tcgtgatggt gccgtccgga
     9901 tcgacgttga tcagaatatc atcatgacag gaccagcggt tgtcgatcat tgctcggagt
     9961 cgccccgcgg agtttaatat gggcaacccg gatttcttca gttgcagcag tttttcgatg
    10021 gcggcgcggc gttcttccgg tggcagcgag agatcgtctt ctccctgatg gtaagggtaa
    10081 aagaactgaa cggtcatccc ttttaatgcg ggtgtgtcct gaacccattg tgctacgaac
    10141 ggcagatcct gccagttgcg gcggttcagg gtgtaatgaa taaaaacacg cgggtgacgg
    10201 gtgttttgaa tattggcgcg gacttgatcg aaggaattgc ttctcaatgt attatgcgtc
    10261 tccctttgac cgtccaaact tacccagagg gtatgggacg gcacatccag aggcatggtg
    10321 ccgttggtgg tcaccgctac acggaggaat ttttttctgg cgtaaagaat aagatcgtga
    10381 agacgataag aaccgtcccg ccacagcagc ggctctccgc cttcgaaaac aatcatccgc
    10441 gtgccgagct gattgagcag atccattatg tgcacggcgg catcccgcga catatgttct
    10501 ttttcgcgat cggaacgcag atgaaaagga catgcccggc agctcaggtt gcagcgatag
    10561 gtgatcttga agctggccag caaaggcagt tttcgcccca gcatttttcg ttgaatataa
    10621 aaccacgcaa gatcaagcag ccagggcgat gaatggatgt ccggatttct gatgtctttg
    10681 cgcaaaatgg tatcacgtgt ctccggtatt caggaaaata tcagccggca aaataaaagt
    10741 tcaagcagct tttttccggt tgaaagatga cgaaattttc ctttagaaac tataggaata
    10801 aacgagaggc atgtcaatac gattcgcgat catgccggtt ggcttgcgct ggccggaatg
    10861 atgaaaaaaa taatggcggc gcgaaaaaaa tctatattat tcaggctttg ttgccttttg
    10921 caatttctgc caatgcatcg aataaaaaat accgtcgatt ttatataagt attgattgac
    10981 atgaccggat gagaccttat attattctca acggaaattc atcaagaggt gggaccatga
    11041 acgatattta ttcggctgtt aaagtgacag atcatgttta ctgggttggg gcgatcgatt
    11101 ggaccatccg ggattttcac ggctacacca cgccgcgcgg cagcacatac aatgcttatc
    11161 tggtgatggc ggatgagatt accctgatcg acaccgttaa aaagcctttt atggatgaaa
    11221 tgcttgcccg gatcaaatcc gtcgtcgatc catccaaaat aaaatatatt gtgtccaatc
    11281 attctgaact ggatcatacc ggttctctgc cggacgtgat tgatttcata aaaccccaaa
    11341 aggtttttgc gtccggggtg gggacaaaaa cattaaaaga tctttttcat gacgagcggg
    11401 aaattattgc tgtcgaaaac ggacagtctc tgaatctggg caatatgaat ctgaccttta
    11461 tggaaacgcg catgattcac tggccggaca gtatgtttac gtatctggat gtcgatgaac
    11521 tgctgttttc gcaggacgct ttcggcatgc atctggcgac gctggagcgt tttgccgatg
    11581 aaattcccgt ttcgaccctc gaatatgaag gagcgacata ctacgccaat attgttctgc
    11641 cgtattctcc tattgtgttg aaagcgctgg agaaggtcgc ggcatcggga ttgaaaatca
    11701 gcgtgatcgc tcccgatcac ggaccgatct ggcgtaagga tatcggttgg attatcgatt
    11761 tatataagaa atgggctttg cagaaaccga cagccaaggc cgtggtgatt tatgcgacca
    11821 tgtggcactc taccgaaaaa atggcgagga tcatcagcga agcgctggct caggccggtg
    11881 tcaaggtcag ccttatttcc atgaacgaaa cgcaccgcag tgaagcggtt tatgaagtgc
    11941 ttgatgccgg tgcgctcatt gtcggctcgc cgacactcaa caacaacatt ctgccccaga
    12001 tggcggatgt gatgacttat ctgaagggtt tgaaaccggt caataagatc ggcgcggcgt
    12061 tcggatcttt cggctggagc ggagaatctg tgaaacatct ggaggccatg ctcaaagaga
    12121 tgaaggttga tattgcgtcc gagtccgttg cggtgaaaca tgttcccggc gccgacgaat
    12181 tggatcaatg tcaaaaactg ggcaaaacgg tggcggcaaa actgctcgag aaggtgaata
    12241 gccagtaaaa tatcgaaata tataaaggag gatgtgaaga tgaaaaagta cgtttgcggt
    12301 gtatgcggtt acatatacga tcctgctgaa ggggattcag aaggcaatat tcctgccggt
    12361 actccttttg aaaaattgcc ggaaaactgg gtctgtccgg tgtgcggagc ggccaaagac
    12421 gaattctcgc cggaatagtg gtcccggtaa aatgatattg cgagcctttt ctcagaaggc
    12481 tttgcaatct tgaaacaaga cttttccccg gcatggctgg actataactt gccggttgaa
    12541 gtggcaaaaa ataaaaggtt ggtaaaacgt atggatataa aggaagcgtt gagtcaggca
    12601 tataccggag aggcgaaggc ggctctccgg cttaaggttt acgcgcagaa ggctgaggat
    12661 gaaggctaca aacaaatggc gaagcttttt cgggttattg ctttttcgga agagattcac
    12721 ggcgcccggg ctctgaaagg tcttaaggaa ataaaaagca cggaagaaaa tcttgcggcc
    12781 agttttgaat cggaaacgca ggttgccgaa gttgcctacg accggtttgt gaaactggcg
    12841 gaagccgaag gcaacaagga ggccgtcctg catttcagcc agagcaagga tgtggaagaa
    12901 acgcacgcca aactttacaa gcaggccatg aaccatttta tggaagaacg cgaaaccgat
    12961 tattttgtct gtaaaatttg cggatatgtc gccgacggcg ttttaccgga ggaatgtccg
    13021 gtctgcggcg cgaaggcgaa aatgttcgtg gcgtttcagg gatgaggttt tttgaagcgg
    13081 gtttgcgata ttgagcaacc gcccggggta tttgacgggc gcaacctgat gaatagggca
    13141 aatacgtgca cattcgatat accgtcacgg tttttttatt gatcatttat ttttcacccg
    13201 ggaaagccat tggcgaagaa aagccgctat gggagattgg tgcgggaatg gcttttctcc
    13261 agatgcccga ttatcggggg tcggatgaaa gcagatttta tccgctgccg tatcctcatg
    13321 ttgtctatcg cggcgatttt tttaaagtcg atgagaaccg gattaccgga caaattttta
    13381 aaacggacag ggtcttgctg gatttcagta ttttcggctc cgtaccggtc aacagtgacg
    13441 acaatgccgc ccgcgagggg atggatgatc tggatccgac gtttgaattg ggaccggctt
    13501 tgaagattaa gctgtgggaa cacaagcaac acaaaacccg aatgaatctg acgctgcccg
    13561 cccgtgcttt tttttcgacg gatttttctt ccgtgcgtca cgagggatgg gtcttcagcc
    13621 cgcggatcaa cattgtgacg gatgatttaa ttccccgtac cggtttaaat ctgggcgttt
    13681 ctgccggacc gatgtttgcc gacagcggtt atcacgaata cttttatgaa gtggaacccc
    13741 gtgatgccag ggtgactcgt cccgtgtatc gcgctgacgg cgggtacagc ggttcaacgg
    13801 tgacaatcgg tttgagcaaa acctataaac actttatctt tcatgctttt gtcagcgccg
    13861 attttcttca gggcgcttct tttgaagaca gtccgctggt gaaaagggaa acgtccatga
    13921 tgagcggttt caacgtttcc tggatatttt ttaaatcgga aaagatggta aacgcggaaa
    13981 aatagccctc cgtgaaaaag ggttgaacgc gttgcctgat ttcagatatt gatgggtcct
    14041 gatatgatta tgattattac aagatggctg atcatcacgg cggcgattct gctggcttcg
    14101 caattcgtgc cggggatcga agtcgataaa ctttccaccg ctgtcattgc cgcctgcgtc
    14161 ctggggctga tcaacatttt tatcagaccg attgtggtga tcctgaccct gccgttgagt
    14221 attctgacgc tgggggtgtt ttattttttt atcaacgctt ttttgctgga actggttgct
    14281 tattttgtat cgggatttgc gattaaaaac tttttttccg catttgccgg ctcgctgatt
    14341 atcagcatcg tcagctggct ggccaattca tttatcacct cgcataaaat cgtcaaaaag
    14401 gacgatccgg actacattga tctgcggaaa ggggacgacg gtaaatggcg gtaggcgcga
    14461 cgctcgattt gactccggcg atgaaacagt atgtcaaaat caagcaacag catcctgatt
    14521 gcattctgct ctaccggatg ggcgattttt acgagatgtt cttcgaggac gcggtgacgg
    14581 ccgcgcccgt tctggagatc acgctcacgt cgcgcaataa gggcaaagag gacagcgtgc
    14641 cgctttgcgg tttcccgtat catgctgcgt ccgcctacat caccaagctt gtggaaaagg
    14701 gattcaaagt ggcgatatgc gagcaggtcg aggatcccaa aaaggccaaa gggattgtca
    14761 aacgggaggt tatccggatc attactcccg gactggtcgt ggatgaagaa aatctggctc
    14821 ccggtgaaaa taattatctg gcctgcctct gcgccgaaaa aaacaccctg ggtctggctt
    14881 ttctggatat ttcaaccggc gaattccaga tcggtgaatt cacggaccgc gattatttct
    14941 tcacggtaat ttccggcctg gattttaagg aattgctcct gccgcgaaat ttccccgaca
    15001 gaattttact cgacaccatc gaagcacaga tgcctcaatt gcgcatcaac gatctggacg
    15061 aaaactattt tctgaccggc caggccgggg atgttctaca gcggacgttt tcaacggacg
    15121 ttctggatgc gtgcggcatc agcgaacatc cggcggcatt gaaggcgtcc ggtgccatat
    15181 tgagctacgt caacgagacg caaaagatca atccgcagca catcagcgcg atcagatggt
    15241 actccgcgga aaagtttctt ctgttggatg ataccgcccg ccgcaatctg gaaatcttca
    15301 cgtcgatcca gaacaatacg gcggccgggt cgcttttttc tcttttcagt gaaacgcaaa
    15361 cgccgatggg gacacgccgt ttgcgctggt ggatgagcta tccgctggtg gatgcagaaa
    15421 aaattaaaac ccggctggcc gcagtggcgg agctgaaaga tgatcctctt cggcgggcga
    15481 atctgcgaaa aacattgtcg cgtatatatg atctggagag gctggccgga agagtgtcgc
    15541 tcaaagcggc caatgcccgc gatctggtcg ccctgaaaaa ttcgcttctt gccgttccgg
    15601 agatcaagtc cctgctcagc gattgcacgg cgccggccat ttgcgccatc cggtcgcaca
    15661 tcacggagat gaccgatgtt gtggatgtga tttcacgggc gattgtcgac agtccgccgc
    15721 agaaaattca ggacggcgga tttatcgctg cagggtatga tgaagaactt gataagttgc
    15781 gattgatcag ccgcgacggc aagcaatgga ttgctgctct ggaggcggaa gaacgcaaga
    15841 aaacaggcat caattccctg aaagtgggct ttaacaatgt gttcggttat tacattgaag
    15901 tcaccaaagc caacgcagct ctggttcccg actcttatat ccgcaaacag accctggtca
    15961 acgcggaacg gtatatcaat caggcattga aagaattcga acagacggtt ctcaacgcgg
    16021 aggaaagaat caagcagaag gagcaggaac ttttcatcaa tctgcgtgac ggacttgacc
    16081 ggtatatcgc ggagattcaa ggcaatgcaa atctgattgc cgatcttgac gccctggctg
    16141 cgctggcgga cgtggccgaa aaatatcatt acgtctgccc ggaaatcgat gatgaggaga
    16201 ccatcgacat tcaggacgga cgccaccccg ttgtggagtc ggctttgaaa aacgaagggt
    16261 ttgtgccgaa cgactgttta atggatttgc gccagaataa actgctcgtc atcaccgggc
    16321 cgaatatggc gggtaaatcc acgtatatca ggcaggtggc cttgattgtt ctgcttgccc
    16381 agatgggaag tttcgtcccg gcggccaaag caaaaatcgg aattgttgat aaaatattta
    16441 cacgtatcgg cgcctccgac agcctgatca agggacagag cacgttcatg gtggaaatga
    16501 cggaaacggc ggaaatcctg aaaaacgcta caccccggtc gctggtcatc atcgatgaag
    16561 tcggtcgcgg aacctccacc ttcgacggat tgagcattgc ctgggcggtt gccgaataca
    16621 ttcatgattt ccagggacgc ggtgtccgcg ctctctttgc cacgcattat catcaactga
    16681 ccgatctggt ggtaacaaaa cccggggcga aaaattataa tatcgccgtg aaggaatggg
    16741 gagaaaaaat aatttttctg cgcaaaatta tggaaggcgg gaccagccgc agttacggga
    16801 tcgaagtggc gcgcattgcg ggcgtgccgg ccgacgtcat tatgcgcgca aaggaaatat
    16861 tgcggaatct ggaaaaaggg gaattcgacg agatcggcat gccccgcata gtccggggag
    16921 cccgcgaagg caaaaacgcc aatccgcaat tgagtctttt ttcaagacgg gaaaccgcca
    16981 ttgctctcga aaagaaagga cagaacctca tcgaggcact gaagaatatc gatatttcct
    17041 ccctgtcgcc tctcgaagcg ctcaataaat tgaacgaact gaagaataaa atttctgatt
    17101 aaatcattta cgattcatga ggaaagcttt caatcctcca tcgttcttta tattccttgg
    17161 cgctcctggc actctttgat ttttttaaga gcttgtttat taacaaaata ctctgaaaag
    17221 gtttattcaa gaaaaagcgg gaggcttaat agcctcccgc ttttgctgag agttgaaacc
    17281 taggagaaga cttaaagggt ttcatggttt gcgtataatt tttatgtgca attgatgtgc
    17341 catagcttgc tgatttttta atatattgaa attacaagca ttattgaaat gttttaaaaa
    17401 atagattgtg ttttataaaa tattatggat aataatgatc caatttacgt atccaattta
    17461 gcattcatct ggataataat tatccagatg gtttcatttt ttaaaaaaga aatgaggtcc
    17521 gtctcatttt tttataaagg atgatgaaac aaagaaccag gggtaataat catggttgtt
    17581 gaaaaagctg tgctgattgt tgacgatgat tccgttcacc ggatgatact caaaaaacat
    17641 ttgaacgact gggggtatca agtcaatgaa gcggacgacg gtcacgtcgc ggtcgaagcg
    17701 gtccggcgaa tgccttttga tctgatcatc atggacattt gtatgttgaa tgtttccgga
    17761 atagaagcct tgggacagat taaagccatc aatccggaaa ccccggtgat tattatgacg
    17821 gcgtacgctt cattggaaac ggcggtcagt gccctgaaaa agggagctta tgattatctg
    17881 accaaaccgc ttaacttcga cgaactgaga attgccatcg gacgggcgac ggaacataat
    17941 cggctgaaaa gggaaaacag atatttaaga aacaggctgg atgaaaaatt taaccggcat
    18001 aatctcatcg gacagagtta cccgatggtc aagctcttgg aaacagtggc ccaggtggcg
    18061 cccacggagg ccaatgtgct gatttccgga gagtcgggca ccggcaagga attgattgcc
    18121 aacgccattc atttcaacag catgcgggcg gaaacggttt ttatcaagat aaactgttcg
    18181 gcgctgacgg aaacgcttct ggagtcggag ctgttcgggc acgagaaggg agcctttacc
    18241 ggtgcggaac gcaggaggga agggaaattc gttcaggctc acggcggcag cttatttctg
    18301 gatgaagtca gcgagatgtc caaggcgatg caggtcaaac ttctgcgtgt cttgcaggaa
    18361 cgcgagttga cacgcgtggg cggagcggaa gtgattaaag tcgatgtgcg ggtgattgcc
    18421 gcttccaata agaacttgaa agaagaagtt cagaacggaa atttccgcga ggatttgttt
    18481 tatcgcttaa acgttgtttc cttgaatgtg ccgccgctgc gcgaacggaa agaagacatt
    18541 ccgatactgg cgcagcattt tctcgagttg tttgccgggc gcaataaaaa aaccatcaaa
    18601 gcattcactc cgcgggcgat ggaaaaactt ttagcctatc actggccggg taacatacgg
    18661 gaactgatga acgctgtgga acggtcggtt gttctgtcac gaacggatta tctggacgcc
    18721 gacgatatga cgctggtcat ggagaaaagc catgaggttt ccggtattgc ggagcagggg
    18781 gaagcgatac gaaatttatc tctggaaagc atggagaaaa aaaatattct ggcggtgctg
    18841 gctgattgca gaggcaataa aagcgaaacg gcgcgccggc tgggcattac cagaaaaacg
    18901 ctgcgcgcga aattaatcaa atatgagatg aaccggtaat gcctcccgtt cagcgggtca
    18961 cgctcttgag aattgatgag acgaaaataa acgctaagaa ccgtttgttg tttttgcggg
    19021 cagataaatg gaaaaggttg ttccggaatt gacgatcgat tcaacggtaa ttaatccgtt
    19081 atggtattta atgatggaat cgctgattgc cagccccagg ccgattcccc cgtgttcttt
    19141 tgtctttttt gtcgtaaagt agggatcgaa gatccgggga agattttccc ggggaatgcc
    19201 gatgccgctg tccttgataa taattcggat gtaattcccc tgtcccagcg accggatgtt
    19261 ggttccggtg gtggtatttt ccgtggtcat ctgcacggaa ccgccatccg gcatcgcttc
    19321 tctggcgttg atcaagacgt tttcaataac ctgtttgatc tgtgtggcgt caatttcgca
    19381 gggccagaga tcttccgcga tggtaatatt gcaggtgatg gccgagtttt taaaaacact
    19441 ttccgccgcc tcatgcaaca caggttctat tttttccagc ttgcgcaccg ggtatcctcc
    19501 ctgagcgaag gtgataagac ggtgggccag ttccttggcc tgaaggccgg cacgttcggc
    19561 tacggcaaga ccgttctcca gcattttatc ttcatcactg gcggatattt tggccaggaa
    19621 aacattccgt aaaatcgcgg tcagcagact gtcaaagtct ttggcaaccc cgtccgcaaa
    19681 ggttcccagg gattccagtt tcgtccgccg gagcagttcg tcctgcgcta atttctgttc
    19741 tgtgatgtcc tccagtgttt ccatgacgcc gaacagattt cctctggaat ctttgaggcc
    19801 tgccgatgtt atgcgatacc atttcccctt ttcgcccatg tcaggataaa actgtgtcac
    19861 ctcgtaggcg tcttccagca gcttggatct gctccagcgg tctggataca gttcggcaat
    19921 ctgggcggtt ttcccgtcca gaatgagatc cgccagacag ggacggggcg atgtgtaaaa
    19981 cgcgcgccag tgctggtctg tgcccagaac gtcgctgggc ttgattttgc ttgccgcctg
    20041 cagcgctctg ttccagtaaa cgactttgtg atttttggat atgacgaacg tggggatagg
    20101 ggagccgtgg ataatgctga tcagtttctg ctcgtcccgg ttcatacgcc acatggtata
    20161 aataaaactg atgatgacgg cgatggaaag aacaaaaacg aaaataaaaa taaaaaaatt
    20221 gttcataaac aggatccgct atgtaaaaac caattcatct tcgatttgat ccggaatgcc
    20281 ttcaatattt aatggatttt gataacaagg ttaaattaaa aatgcaagaa ctgaaaataa
    20341 tttgtttcaa ccgggttttt tgccgttaaa aacatcaagc agcattctta aaagatgctc
    20401 aacagtggaa aacccgatta gcgatatcag atgcttgaac aaagataaag atgcaacttt
    20461 ttcgctgttg atgaagggaa atcgtgtgcg aaagaatttt tacagatctt cgggaaaatt
    20521 tccggccggg gaaagatgat cttatccgtt gtcttcaaaa cggtaaagat cgccgaaaga
    20581 cacaccaaac ggcttcctga tcaaaacgga tggcggaaag ataattaacc ggctcagatg
    20641 ttatcggaca gccagtcgcc gatttcgctg gtggacatgc ccatcttgcc ggcatcgagg
    20701 cttttgagtt tcccgctttc cagaagttta acgaccgcgg cttccactct tttggctgcc
    20761 ttttcttcac ccagcgtttc cagcagcata ccgccggcac agatggccgc cagaggattg
    20821 atgatattct tcccggtata tttcggcgca gaaccgccga tgggttcaaa catggagacg
    20881 ccttcgggat taatgtttcc gcctgctgcg atgcccatgc cgccctgaac catggcgccc
    20941 agatcggtaa tgatgtctcc gaacatgtta tcggtcacta tgacgtcaaa ccattcagga
    21001 ttttttacca tccacatgca ggtcgcgtcc acattgacgt aatcggtttt nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taaagtttga caggccggag attaatgtac
    21181 tggtcgagct cgaaacgcat tcgcagcaaa atgccttttt ccaggattcc cggtttgaca
    21241 tccggatgtc cgatcgcacc cagataaatg gcatcaaatt tcctgaattc ttcgagaacc
    21301 gaatcaggca ggatctcccc cgttttttta taacgttcgc cgcccagatc ataatgaacg
    21361 agttcgtatc gtatggattc ggatgccgcc acggctttga gcactttcag gccttcggca
    21421 acaacttccg gaccggttcc gtcgccggga accaccgcaa ttttataaga ttttgatgac
    21481 gtcacagaaa cctcccggat catgttcgat catgaaaaaa taaagaggcc gcactcataa
    21541 cgcaaggaaa ggtcaaagtc aatatttaag ccgttgcgtt ctccaaaata gttttcccgg
    21601 cccttaattt ttgaaaacag aaggggagat gataataata cctttattct gataatctac
    21661 aaagatatca aatcattatg gctgtgaagg gtagaaattc ttttctgatt ataccttaaa
    21721 tatttgcaag taaactcaaa gaatgttaat caataaagcc agcaattcat actggcaaaa
    21781 cgtgcccgca cagtcaggct ttttaactaa tgtggaggtg attatgggag aaggaattgg
    21841 cgattacaca aaaaacatag ttgatttaag aagacatatg tggatggccg gtgtggaggt
    21901 tgaacttcgt cgtctcatct ggcagattgc cgtcgtcggc aaatatatct cagcaaagat
    21961 tcatgagtcc aacagaaagc ttgccggact gaagaatgtc tacggcgagg aacagatggc
    22021 gctggatcgc gcttccgacg agatactgaa aaaccagctg gaattttcgg gttttgtccg
    22081 tgaatacgca tctgaagagc tggagaatat cgtaaaaata ggcaccggtc aggaaaaata
    22141 catcgtaacg gctgatccgc tcgacggctc atccctggtg gataccaatc tggcgatagg
    22201 aaccattatc ggcattcaca aggaatcggt tctggattgc ggacgaaata ccatggtggc
    22261 tgccctgtat attacttacg gtcctctgat tacgatgatt tattcggcag gcaagggcgc
    22321 ccatgaattc gtccttaatc gcgagggaga gtatgttctg tcgcaggaaa atatcaaatt
    22381 gaaagaaaag gggtcgatat tttcaccggg cggcctgcgc cgggactgga atccggcgca
    22441 tcttcagttt attgacaaac tggaaaacga cggctacaaa ctccgctaca gcggcggttt
    22501 cgttcctgac atcaatcagg ttctgattaa aaagggagga ttattcacgt atccggcttt
    22561 aaaaaaatcg ccgaaaggca agctgcggct gctgtttgaa ttgcaaccga tggccttcat
    22621 catggaacag gccggaggcc tcgccatcga cggcgatcag gatattcttt ctgtgaaggt
    22681 ggagacgatc aatcagctct cgccgatcta tatcggaagc acttacgaag tccaactggc
    22741 aaaagaaatg ctgtcggcat cgtgaaaaat aatcccggtg cggagatggt cggaacgggc
    22801 gggtgatttc cccgccggcg actgaataaa atataattgc aaggagtgat tatgatttac
    22861 aaagataaaa atgaattgca gaagaaatac gaaaaaatta ttgccatcga gggcaacgat
    22921 gtgagcattt tgaatgagac ggcactcaga ggagacttga ttgatcattt gatttatacc
    22981 gccgtgttca gcgccgatca ggcagcacag caagccgcat gctggctgat ccgccgcgca
    23041 ggcgctgccc tgggaattgt gtccgcctcc attcaacccc tttatgaagc gatgggccgg
    23101 gaagacgtca ggggatttac cgttccggca atcaatctcc gcggcctgac ctatgaatcg
    23161 gcccaggcgg ttttccgcgc gatgatgaaa gggaaagtcg gtcctgtcat tttcgaaatc
    23221 gcccggtcgg aaattgatta tacctcgcaa cggccggtgg aatacacagc ggccgtcatt
    23281 gctgccgcaa tcaaaaccgg atatcgcggg cccgtctttt tgcaggggga ccattttcaa
    23341 atcaacgcca agaaatttgc ggccgacgca gcgaaagaaa cccagacggt aaaaaattta
    23401 atctgggaag cgattgaagc gggattttac aatatcgata tcgatacgtc gacgctggtg
    23461 gatctttcca aacccaccgt cacagaacag caacagacga atttcagtct cgcagccgaa
    23521 ctgaccacca tgatccgcga tctggaaccg gagggaatca caatatcagt cggcggcgaa
    23581 atcggtgaag tgggcgggaa aaacagcacg gtggaagagc tggaggccta tatggacggt
    23641 tatctggcgg agcttaaaaa acagggtgaa tcgctcaaag ggatcagtaa aatcagcgtc
    23701 cagacgggaa cgacgcacgg cggcgttccg ctggcagacg gcagcgtcgc caaagtcaaa
    23761 atcgattttg cggttctgga gaaattatcg gcggtggccc ggtccaaata cggtctggcc
    23821 ggtgcggttc agcacggagc gtcgacatta cccgacgagg cgtttgaccg gttccccgcg
    23881 gtggggacgg ctgaaattca tctggccacc ggctttcaaa atattattta cgacagttcg
    23941 gctttcccgg ctgacttgaa ggccagaata tacgatcatc tgaaaacggc attccagtct
    24001 gaatggaagg ataaagatac ggaagaacaa tttatttaca aaacccgcaa aaagggtttc
    24061 ggtcccttta agcaggatat gtggcatttg ccggcggaca cccgcgataa aatcagcgcc
    24121 ggactggaaa aacagtttgc ctttctattt gacaaactga aaatgaacaa tacgcaggat
    24181 gtggttgaaa aatatgttca acccgtggac gtcgcgccgg atgttcctgt cagcctgaaa
    24241 tgaaagcggc gattttagag caaacaacca gcttaaagaa aaataagaag cctctgaaaa
    24301 taaccgaggt tgccgaacct gcggtgggcg acggtgacga cgtgctggtt aaagtgtcgg
    24361 tatgcggcgt ttgtcacacg gaactcgacg aaattgaagg cagaatcttt ctgccttcat
    24421 tccctgttat tttgggacat caggtggttg gcgttgttgc cggacagggc aggcgcgcgc
    24481 gcaaatttgc aatcggcgac agagtgggca ttggctggat taatcgatcc tgcggtcaat
    24541 gtcctttctg tctttccgga aatgaaaatc tatgcgccgg ttttaaggcc acgggcaggg
    24601 atgtctccgg cggctatgcc cagtatctga ccgtatccga aaaattcgcg tttcccattc
    24661 ccgaaatttt ttccgatagt caggccgcgc cccttctgtg tgccggcgcc atcggttttc
    24721 gttccctgcg tctggcaaat ctgacaaacg gacaacgtct cggtctgacc ggattcggcg
    24781 cgtcggccca tctggttttg aagatggccc ggtatcttta cccgcaaaca ccattgttcg
    24841 tgtttgcccg caatccggcg gaacgtcaat ttgcccttga cctgggtgcc gtctgggccg
    24901 gagatacatc cgacgaatct ccggaaaagc ttcatgccgt cattgatacg acgccggtct
    24961 ggaaaccggt ggtcgaagcg ttaaaaaatc tggaaccggc cggccggctg gtgattaacg
    25021 caatccgcaa agaggacaca gacaaagatt atcttctgca actgaattac ggacgtcatc
    25081 tctggatgga aaaagaaatc aaaagcgtgg ccaacgtggc cctttgtgat attgagaact
    25141 ttcttaaact ggcctccgac atgcgcattg ttccggacgt gcaggcctat ccgctcaacg
    25201 acgtcaatca agcgctcggc gaattgaaag aaggtaaaat ccgcggagcc aaggtcttaa
    25261 taatcagttg atttttcaac ggcaatctgc gtattattaa tatattaatt tgtaaatttt
    25321 tattaacgct accataaccg gaaaccgcaa cggatttaaa gtttgtggct cttttttatg
    25381 ccggataatt ggcaggaaat gggatcgagg tttgcggaga actcattatg aaaatagcca
    25441 gtgtcggtga aatgcgttcc atggatcgct ttgctattga gcatctgggc attcccgagg
    25501 acgtactgat ggaaaacgcg ggacgggcag ccgtcagcgt tcttttgtcg cgcacaggca
    25561 ttcgcggcaa gaaattcgcc attttctgcg gatccggtaa taacggaggg gacggtcttg
    25621 tcgttgcacg tctgctccat tcacgcggcg gccttgtcag ggtattttta ctcaatgaca
    25681 gcgaaaaatt cagaggtgcg gccgcgacga atttcgaaat cgtcaaacgg ttgccgctgg
    25741 aaacagcgca gctgacaaat gcccgaccgg ctggcaggca cgtggcccat tgcgacgttg
    25801 tgattgatgc catcctgggg acgggattgg atcgtccggt ttccggattg gttgcggaca
    25861 ccatttcact gattaataaa ggcggaaaaa aagttttatc gctcgacatt ccctccgggg
    25921 tcaacggcaa taccggagaa atcatgggag ccgctgttac agccgactat accgtcacgt
    25981 tcggccttcc caaaatcggc aacctgcttt atcccggttg tgacgtcaac ggcgaattat
    26041 gggtcagtca catttctttt ccgccgtcgc tgtatgaccg ggatgacatc ccggtgagga
    26101 cgaatgacgc tatggctttg ccgccgaggc ctgccgatgc ctataagggc gccatgggcg
    26161 atgtcctgtt catcgcagga gcggtcaatt attacggagc tccgtatctg tcggccatgt
    26221 cctttttgaa ggccggcggc ggttatgcaa gactggcagc gccgcgtgcg gtgattccgg
    26281 taattgccag gcgcggccgg gaaattgttt atctgccgca ggaagaaacc gaggcaggga
    26341 gcatggcttt gaaaaacaag aaggcgttgc tggagcattc ggcaaaggcg gatatggtgg
    26401 tcatcggtcc gggactttct ctgcaggacg aaacggtcaa ccttgtcagg gaaatcgctg
    26461 cgacggttcg taagccgatg ctgattgacg gcgatggtct taccgctgtg gcaggctgtc
    26521 cggacagcat tcgccggcgg aaagcggcaa cgattctgac accgcatatg ggagaaatgg
    26581 cgaggctgac gggcaaatca ttgaaggaaa tccatgccga taaaataaaa gtattgcggg
    26641 agacggtgca aacattgaac gcctgtatcg tgctcaaagg accgcattcg cttatcggca
    26701 cccgtgacgg gaatgtttac gttaatttaa gcggtaatgc gggaatggcg acggccggtt
    26761 ccggtgatgt gctgaccggc tgcattgcgg cgatgttcgg catggggttg tccttcgatg
    26821 aagcaacccg caagggggta ttccttcacg gggtcgccgg agatctggcg gcgttaaaaa
    26881 aaggaacgga cggcatgacg gcgcaggata ttatgaatgc gctgcctcag gcgctgaaag
    26941 aagacaggtc cggaaggctt caggatcaat atgcccttcc cgacggcgtt taataaatgt
    27001 tgtaaaatcg aacagggcgt tttacgatag gctatgaatc ttccaatgca aataaatcgg
    27061 ctgaccctca aattttccgg ggaattgtct tatctcgaag cgccgtttct gaaagagtat
    27121 tatacgtcgt ctctgccgca catccgcatg atgatgattc tgggagcgat gctttatgcg
    27181 gcgttcggtg ttctggatgt tctcctgatg ccgcagcaag tcgttgcgct ctggatcatt
    27241 cgtttcattg tcatcggtcc ggtgctggtg ggcgtgctgg cgatatcttt tttgcccata
    27301 tttgaaaaat acatacagcc catacttgca ctggtttatt ttctggccgg tgccggcatc
    27361 atcggcatga tcgttgttgc accgccgccg gtcagctatt catactacgc cggccttatg
    27421 ctcgttttca tgtggggata caccctcatc cggctctttt ttacctgggc gtctgttgcc
    27481 ggatggctgc aggttatctt ttatgaaacc gttaccgtcg ggctcaaccc gacgccgctg
    27541 gatgttttcc tgggcaataa tttcttcttc atcagcgcca atctggtcgg aatgatggcc
    27601 tgctacgcga ttgaatttta tgcccggcgc gactttctca tgaaccagca ggtggaagag
    27661 gagagggaaa aagtctccag gatcaatgag gaactggaag accgggtcaa acagcgtacg
    27721 gaagattata aaaaccttaa tcaggcgctt ttgcaggagg ttgccggtca tgagcaggcg
    27781 aaagagaaac tgcaggaagc tctggacagt ctcaagaaat ccatgggagt aaccattcag
    27841 gtcatggtgt ccgccgtgga ggcacgggat ccgtatacag caggccatca gatcagatcc
    27901 tccaatctgg cctgtgcgat tgcagcggaa atggggttgc cccaggaaaa aattgacggt
    27961 attcaaatgg ccagttccat tcatgatatc ggaaaattgt ccataccggc ggagatactg
    28021 acaaaaccga caaaattgac caatctggaa tattccctca ttaaggagca ctcggcctgc
    28081 ggatatgaaa tgctgaaaaa tgtggaatcg caatggccgc tggcggaaat catttttcag
    28141 catcatgaaa ggatgaacgg ttcgggatat cccagacagc ttaaagggga tgaaatactc
    28201 cttgaagccc gtattctggc tgtttccgat gttgtggaat ccatggcgtc ccatcgtcca
    28261 taccgtccgt cgctgggaat cggagccgct ctggaagaaa tcgaaaaaaa cagggggatt
    28321 ctctatgata gcgctgtcgt ggacgcctgt ctgaagttgt tcagagaaaa gaacttctac
    28381 cttgagtaaa gtttctgcca ttgaagatgc ctcattaaat tgtttcgtga gaactgaaaa
    28441 atgacaaaat cattcgctga aaatcgtgac acaaaaaaga atttgaaaaa atattatatt
    28501 atgtacgatc ccacgaccgt ggatctgatg ggaaaaatag gcgtggcggg cttcaccgtt
    28561 gaagatacgg ttgaatacct gacgggcaga aagctggaag cgtgcaatga aacgaattat
    28621 accgaattcc tgagcttcaa taccaacctg ctccggaaag ggatgctcga gcagatggcc
    28681 atcgagccct ataaagccta ctatattgcc aatcagattt gccgggcgga tcaagacgca
    28741 agagtcatca tcgccgatga tacccggaaa acgatcggac agatcattgc ggaagaaaaa
    28801 caaaaaccgg tggcagtctt catgtctctg atcagttcaa cgtttcccac cgcttgtgcc
    28861 gtgattctga ttttgaacaa agtcaacata ccggtcgtca tcggcggcat ccatgtttca
    28921 accagcttta atgatgtgga catgtatctg agaaagtatc tgccgcatcc ccaaatcgtt
    28981 acgcaggtta tcggcgccgg cgatttgccg accattaaaa acgttatgtc ggatcttgcg
    29041 cgttcgaatc tgaaaaaaga gtatcacgga aatatcccga ttgaggatgg cgtctgggga
    29101 tgtgaccggg tcatcgaatt acctaaaatc aaaccatact ttattaataa atttcccgtc
    29161 atcggcccgc tcctttcacg aatcattgaa acgaacgtga cgactccctt tttaggctgt
    29221 ccgttttcct gcagtttctg ttccatatct tcgttcccgg aagagcgaag aaagttcaca
    29281 tctcgttcac ccgaggattt tacctgtgaa ctgctggata agcagaagaa aggtgtcacg
    29341 tttaagaacc ggttctattt catctcgccc gataaccttt tgttcggggg gaaaaagctg
    29401 cacgatgttc tggacaaaat gatcgaaagt cctttacgga tcaattacgc ggcgcaaatc
    29461 agcattgagg tcgccgatag tgacaagctc cttcaaaaac tgaggctttc cggcgcatcg
    29521 catttcttta tcggattgga atcgctggat atcaggaatc tggagtatgt cggcaagaat
    29581 atcgtagcca aaatcaaaaa agagaaaata tccgtcgaag agtattacgc atcgcgaatt
    29641 aaaaaaattc aggatcacgg tatttccgtt catggtgcgt tcatgttcgg aatgccctac
    29701 gattattttc attctttaga ggatcattcc ggcaggaaaa ttgttgaatt ctgcaagaag
    29761 aataaaatcg gcattcagcc gacctgcctg agcaatctgc cgggctcgct tgattttctt
    29821 aaaggcctgg agacgcatga gctgatctac ggcaatcccg gttcaatgga ttatttctgt
    29881 tcgttaacca ttgccgattt gacggagagc aaccgaaaga taccggatgc cctgttcaac
    29941 agtccgctgg tgacttttta catgctgtac gacaccatgg aaaaaatagg atcctacatc
    30001 agcgctttgc atattgcttt tggtatggcg aagaaatcct ggagtgtcca ttcgtcaaaa
    30061 ggggcgcaaa gcttcaaaga ccgcatggtg gatgcttttg ccggcattgg atttcaactg
    30121 ggggcgagtg cctactttga gctgtacaag gggcttgcgt attccacaag ttcgcttccg
    30181 ggaacatttg aaagattata tcaatgggaa aaaaatccaa ccgtgaggaa gatgttcaaa
    30241 aaatacgtgg agaattttgt ctagagcggg aattgcattg tccaaaatca cacgcagcga
    30301 aaaagaaata gaagaggtac gaaacgccat tctcgaagaa gccctgacga ttctgagaga
    30361 agaaggattt gagaagcttt ccatgtacca actgggcaga cgaaccaata tgacggcggc
    30421 caacctctac aattatttca gaaacaaaga tcaactgatc atcgcaatcc ataaaaaaac
    30481 atttgagttg ttatatgcca tgctttcgaa ggctgtccgg aaagaagcgg agccgatggc
    30541 caaactgaaa aaaataatca aaacattttt gacgtttgct aaaaaatatc cgaattatta
    30601 cgacatcatg tttaatcgcc gggtgcctca ggtatccgac tattatggaa catccgaaga
    30661 atctcttgcc cgcgaggaat atgaaagctc ggcgagaaat cttaatctgg tcatgagtat
    30721 cggcaagaac gttctgtcct catcttctga gccggatgtc cgggacgtgg agttttattt
    30781 tatcaggacc tttagcgaac tgcatggcat tgtctctctt tacaacagcg gtattttaaa
    30841 ggtgatcgtt aaaaatcccg aacgtgcaat gcatcgtatc gtcgatgatt tactgactgc
    30901 ctacatcagg aggaaatacc ggtgatctca tagaaatatc agacgcaaga tttgacttcg
    30961 gctcaacggc ctttcctgcc aatccggtcc gctgttgcgg tggcggcgga tgattctttt
    31021 tcgatacgtt atcgctatca tcggtttctg aatcctgacg gaaaacgatt gacttttaaa
    31081 tttaatagcg ttaaattaaa gacaaaaaac tgacgcttct gcaagggatg tcaggcaaga
    31141 gaaaaagcca tgcaaaacac gctggaaaac agttcagatc aaaatcggcg atcaggctat
    31201 tggggtctgg cggtaagcgc atcaaaggtc ggcatcggta aagtgctgcg caagcatcag
    31261 ttgcttctgg aagggtttct gcaatttatc gccggccgat tcaatacttc cgaagcgctg
    31321 ctcgaggagt tgaagggcac gcagggctgg atcaaccttt gtctggggat gagatcggag
    31381 gacagcgcca tcgaagcggc gatcgtgttt caggatggcg tggtccgtgt gctgaagaca
    31441 attcctgaaa atgtggaaac ggtcattatc tttaaaacct ctgatcatct tctggatatg
    31501 accgatgcaa cccccgatga aatgtataaa atgatgctga tcggcaccat tcgaacacaa
    31561 ggcaacatca tgctcgcctc cctgttcaat tatctgatgg cgcttgtttt tgacaaggga
    31621 cagcagaaag ccgtggaccg acagattgaa gaacatagaa aggcaaacaa atccgtcggc
    31681 cgggatgtgg ccgacaccga ttgctgcagg caggaacgtc agagaagaaa aatcagccgc
    31741 gtagcgggcg gaaaagttga tcccggcgtc aaatatctgg aggacccgca tctggccggc
    31801 ttagggctgg aggattttcc gaggctggaa caatttcgcg ccgagtattt cggtaaaaaa
    31861 aaggaagcgg tggtctgtca cgaatacgga aaactgatca cagatttcca tctggccaac
    31921 ggatacgagg tggataagga cggcaaaccc tgggatccca acctgcgtaa agcggaaagc
    31981 ctgaagtata tcctggagaa cagaacgccg gtgatcagga caaacgatct gctggccgga
    32041 acgtacacca catctcccgt ttccacctgc gtcggacatc ctttctccat cggctgttac
    32101 agctggggtg agctgcggtc cttttcaaaa agggagctca tgccgtatga gatcagcgag
    32161 gaatccattc atattctgca caggcacgta tttccctatt gggcaaaaag aaatattcac
    32221 gaattgtgga gaagccggac aaacggcggt ctgcccgttc agattcatga caggtttttt
    32281 tccgtttatt attggaaaac gatatccatg tcggaagtgc ccccgggaca cgaagcgctt
    32341 atcaagctgg ggaccggagg gttgatcaga aagatagaag aagaacttgc cagagatgct
    32401 catgccgacg atgaaaagaa aaatacgtta aaggcgatga tcatcagtct ggagggtgtc
    32461 aacgcctacg cccgcaatct tgcccgtcag gcgcttgaag agtccaaaac ggcaacgaac
    32521 ccgcagcgta aagccgaatt ggaaaccatg catcggatgc tgctcaaaat tccggaaagt
    32581 ccatctgaaa cgcttcatga ggccgttcag aatattattc tgatgcacct ttgtctgggg
    32641 atggaaagca ccgacgacgg tcccatgtac ggaaggctgg atcagattct tcagccgtat
    32701 tttgaatcgg atatgcataa actcaccacc ccgcaaaaac gggaagcata catcaaacag
    32761 gtgatcgata ttctcggatg tctttacttt atagaatcca gtcatcagat tcttgctccg
    32821 gatatcggca actggcagaa cggcggctcg tcgcccaacg gcacgatcac gctgggaggc
    32881 gtgacgcctc agggggaaga tgcggtcaac gacatgacat atatccttct caaggtgacg
    32941 gaattgcttt cgttgaataa tcccaatgtt catgcccggt ataaaccgga taaaaacagt
    33001 tttgcctatc tgaaaagggt ttgcgatgtg aattacatca caggcgcgac gccctgcatc
    33061 cacggcgatg acgccgtcat ggaatccttg acggcgcgcg gctgggccgt tgaggatgtg
    33121 agggattggg ttgtcaacgg ctgcgtggaa ccgggcattc ccggcaagca ctgcagcgcc
    33181 acgtccagca tagaattcaa tctggtcgcc gtactggaaa tggcgctgaa taacggaaaa
    33241 catcccctga tgaactggaa acttggtccg gacacaggca tcatcgggca gggcgatttc
    33301 gagacctttg acgatttctg gaaagcgttc aaagaacagt gtgaatttct gtgcgagcaa
    33361 tccattatcg gcaataacca attgggcgag atctatcaac agcatcagcc cgcgccgctg
    33421 atttccagca tgaccgaagg gtgtattgaa agcggcagag gggtaacccg gggcggagcc
    33481 agatataatt cgtccggcgt atccgttatc ggaatggcgg atgttgtcga ttctctgatg
    33541 gcgatcaaaa cactggtcta tgatgagaaa aaaatcacgt ttgccgaact taaagaggcg
    33601 attgacagca atttcgccca tcacccccgg ttgcatgtca tgattaaaac cagagtgccg
    33661 cgtttcggtt cgggcaatga agaatccgta acaatggcca accgggtgac gtcattggtg
    33721 catgattatt ttgcctctca ccaaaattat cgaggaggac cccacgcgac cggttggtgg
    33781 tccatggcca atcatacggt ttacgggcgc gtcacgggag cctccccgtc gggccggttg
    33841 gccggcgaac cttttacccc cggactcaca ccgcatccga cggcatcgcc gaatctgctg
    33901 gataatctcc gggacgtggc gaaattgaaa cccgaaacgc tggataataa catcgccttc
    33961 aatgtcaaaa ttgttcccgc ttcctcggac agtcatgaaa ggattattga aatcatggcc
    34021 aattacgcgg atacgtattt caggatggga ggcatgcaga cgcagttcaa tgtgatttcc
    34081 acggagacgc tgaaagatgc gatggcccat ccggaatatt acagcgattt gatggtcaga
    34141 atttccgggt tcgtggccta tttcacaagg ttgcagcgtg atttgcagct ggaagtcatt
    34201 cggcgcgctg aatataaaat atagagttta tacgtttcgg gagagaatgt aatcggcaac
    34261 ggtaatcagg tgtttttttt cggcgcaatc ttcaaaaacg tcgagttgcc gtttggcctc
    34321 tttgatgtat cccgccgccg tataccgcga caattccagc ccttcgtatt tttcaatgaa
    34381 tgtccggacc tgcagagcgg ctttcctgga gctttttttt cgggagagga tctctttcat
    34441 ggtgtttttt tcttcgtccg tgctttgttt gagggcaaag atgagcggca gggtgatttt
    34501 gccttcctcc agatccttgc cgatcgattt gccgaatgtc tggcctttgg ccgcgtaatc
    34561 cagaatatcg tccgtaattt gaaaagccat gcccgcgttt ttcccgaacc gggccatggc
    34621 gtcgatcttt tccggggatg ccgagcccag gatcgctccc gcggcgcagg atgccgaaat
    34681 cagaacggcc gtcttctttt caataatgtt gagatagtca tcccgcgtca tgttgatatc
    34741 cccgcatttg gtcagttgga aaacttcgcc ttccgacatg atatttccca tccgggagac
    34801 cagttcaaaa atattgttgt tgccgatttc gctcatcagc ataaaggcct tgaaacacag
    34861 aaaatcgccc accagaatac tgacggaatt tccgaatact ttattggcgg acgttttgcc
    34921 gcgtctgatc agggcctgat caatgacatc gtcatgcagg agtgttgccg tatgaatgta
    34981 ttccatgacg gtccccatgg cataacggtg ttccccgtga tagccgcaaa gccgggacgc
    35041 gatcagcagc aaaaggggac ggaatcgctt cccgccgctg tcgatcagat gatgagccac
    35101 ttcgggaatt aattttattt cagagattgc attgtcactg atgcattttt cgacctgttg
    35161 aagatcctct ttgtagatgg aaaaaatttc ttgaatttgc atcgtaaaat ccgccttgtt
    35221 gtggttacca gggatcgaca taattatgaa cggggctctg tttttttccc gttgctgcgg
    35281 attttatgcc ccgcatgatc cacttccggg tctcggacgg atcgatcacg gcatcgattt
    35341 ccaggtagga tgcgatgttg acggccttgc cccgcttgta aagttgggcc accaggtccc
    35401 ggtatagctg ctcacgctga tccggatctt cgatggccgc cagctctttt ttaaaccccg
    35461 ccttgatggc cccctcaatg cccatggcac cgaattctcc cgtgggccag gaagccgtga
    35521 agaaggattc atggaaacct cctttggcca tcgccatggc gcccaggccg taaccgcggc
    35581 gcaggacaac cgtaaaatat ggaacggtca ggtgcgagcc gacaacaaac atccggcaga
    35641 catggcgcac ctgcgcgcgt ttttcgattt ccggacccac cataaatccg ggagtgtcac
    35701 acagagacag aatcggcagg ccgtgtgcct gacaaagctg catcagccgc accgttttgt
    35761 ccgcgccctc ggcttcaatg gcgccggcca tatgcaggca gttgttggcg atcaatccga
    35821 aaggccgccc ttcgatgcga atcaatcccg taatcatccc cgcgccgaat ttagggcgca
    35881 gttccagata tgaatccgta tcggcgagcg ctttaatgac ggttcgcatg ttgtaggcgc
    35941 gcctgttgtt ttcgggaatg aggtcgcgca gctgaagcgg atccgccgct tcccatgacg
    36001 gcgttgtccc ctgaaaatag gagagatatt tttttgctgc ggcaacggct tccacgtcat
    36061 cggcaacttc gatatctacc acgccgtttt gtgtctggac gtcaataggc ccgatctgtt
    36121 ccggtttaaa atttcctagg ccgccgcctt cgatcatcac cgggccgccc atgccgatat
    36181 tggaattttt cgctgcaatg atgacatcgc agcagcccag cagcgccgca ttcccggcaa
    36241 aacaggggcc ggacacgatc ccgacaagcg gcactttgcc gctgagtccg gcgaacatgg
    36301 caaacgttga caaatccagg ccggcggtca tcacaccgat ggcgtcgacg tctcccggcc
    36361 ttccgccgcc gccttccgcg aacaggacca gcgggacaga ctgttcatgg gcaagtttga
    36421 gcatccggtc catttttttg tgattgaaaa atccctgcgt gccggccaga accgtgtaat
    36481 cgtaggccat gaccatgcag cgggcctttt cttctttgaa gaaagtgccg ttgattgttc
    36541 cgatgcccgc aatcagtcca tcggcgggtg tcctggccat gagatcctct tcggaacgtc
    36601 tgccgcgctg cgccgccatc gccagcgctc cgtattccat aaaacttccg gcgtcgcaca
    36661 gatcctcaac atttgcccgg gctgttcgtt gatttttctg tctgcgtttt tccaccgcct
    36721 cgggtctgtt ttgatccaga ccaaatttct gccgtgcaaa catttcggca agttccggcc
    36781 gcacggatgt cgtttcggtc tgctttgtat tgaaggagac gccggtttgt tcagtttctt
    36841 tccggtctgg agatgtcgtc atgcgcgttc tgctccctgg gttgccccga cgtgttctat
    36901 gtcaaaattc atacaacaat tataggggta tttcaaaggg taaattcagc atttcttgcg
    36961 catcatgcgc ttatcggtcg gaagagaaac cgacgggcta tgaattcatc cggaaatgtg
    37021 agataatttt aaagtattga aagatgacgg caaatcttct gaaaatattt aatccggtga
    37081 aaaggaacaa aacggttttg atgaaaaaat cactctcctg tctgcaatgc ggaaaatgtt
    37141 gtttcgtcga ttttacggct tatgcgcagc aggaagatta tgaccgctgg cgagccgaaa
    37201 acaggcagga cattctggag atgatcgacc atcgccattt gttctgggcg ggcgacagaa
    37261 tgatatcttc ggatacgggc aatgcgccgg gggaatgtcc cttcctttac aatacaggca
    37321 aggtctggct ctgctccata tacgaaacaa ggccgttaat ctgccgtgac tatcagcccg
    37381 gatcgtccga attatgtccg cagtggaaaa acagaaaaaa aaaggaagaa taaaatccag
    37441 gggctaggaa gacttccgga tgatctttcc aaaaaggtca tagtcgtccg ctgcggttat
    37501 ccggcatttg acgatatgac cgatgtccgg cgaaggcccc ttgatatagg taaccccgtc
    37561 gatctcgggc gcttggcggc ggcatctgcc gatataagga taatccgggc ggtcgctttt
    37621 cccttcaatg atcacctcct ggacggaacc gatgagcgac gtgttgatgg aagcggaaat
    37681 ttccatctgg tcatgcatga tcgtttcacg gcgccgttct ttttcctttt ccggcacatg
    37741 atatcgcaag ccggcggctt ctgtgccttc ttctctggaa taggtgaaaa caccgagatg
    37801 atcgaaccgg gtttgtcgga caaaatccag catccggttg aaccgggccg gtgtctcgcc
    37861 ggggaacccg gtaatcaggg atgttctcag ggcgacatcg ggaatgatat gccggatttg
    37921 agcaattacc tgcctgattt ccgcagcgtt cactttgcgc ttcatggcgt tgaggatggc
    37981 atcgtcaata tgctgaatgg gcagatcgat gtaggaacat attttatcgt tttcagcgat
    38041 cgtgctgagc aaatcggcgg taatacgggc cggatgcgca taaagcagcc ggatccattc
    38101 gatcccctta atttgcgtca gatcgttcaa cagatcggaa agcctgggcc gccctttcaa
    38161 attccagccg taaccggtcg tgtcctgcgc cgtaatgatg atttccctga tgcctctttg
    38221 ggccagtcgt tcggcttcgc ggagaatatc atccggcgga cggctgcgcg ccgcgccgcg
    38281 cagggaaggg atgacacagt aagaacagcg gttggagcat ccttcagata tcttaaggta
    38341 ggtactgaat gccgatgccg gtaaaatacg ccggtggttg cagttcatta aataaaccgg
    38401 ccgggtgatc acagccttac ggcggttttt gcaccggatg agttctgtga cgtgcctgac
    38461 aatgttgccg acctcgccgg ttccgatgac ccgatccgcc tccggtatct gatccagaag
    38521 ttccctgccg tagcgttgtg cgagacatcc cgccacgatg atccgcaggt tcgggcggtt
    38581 tttttttgct tcggcgacgg tcaggatttc atcaatggct tcttctttgg ccggattaat
    38641 aaaagcgcag gtattgatga tgacgatatc cgcgtcggta tgtttgtcaa ccagggcatg
    38701 gccggactgg cggagaaatc ctcccatgac ttccgaatca atcagatttt tcggacagcc
    38761 cagactgata atattgattt ttgttttctc tgcgtttttc aaccgggaag ctttctaatc
    38821 agaacttttc tgggtttggc gccgtcggaa ggaccgacga tgccttcgga ttccattttt
    38881 tcaataatgc gggcggcgcg gttgtaccct attttcatgt aacgctggac cagtgaaatg
    38941 gaggcctgtc ccagttcgct gaccagtgcc acggcttcat cgtattttgc gtcgaaatca
    39001 tcgtcgcctt catgctggat ctgcgcggcc gccagggcat atttttcgat ggaatcgtcg
    39061 tatgcgggct gtgcctgcat tttgataaag tcgacgatgc gggaaatctc cttgtccgaa
    39121 acgtatgcgc cgtgaatgcg ggagagtttg gaggtcccgg gaggaataaa aagcatgtcg
    39181 ccggatccca gcagcttttc cgccccctgc tgatcaataa tcgtacgcga atcgattttg
    39241 gaggacacct gaaacgaaat gcgcgtggga aaattggctt taatcaggcc ggtaatgacg
    39301 tccacggaag gccgctgcgt ggcgatgatc agatgaattc cggcggcccg cgccatctgc
    39361 gccagccggg tcagcgattc ttccacatcc cttggcgcaa ccatcatcaa atcggccagt
    39421 tcatcgatca cgacgatgat ataaggcagt tttgtgtgct tgatttcctg ttcttcccgg
    39481 gccgtttcat cgatggtttg tccggattcc agttcggggt cgatttgttt cggcatggcc
    39541 agcaccgttt tgcttttcag ccctttgagg agcagctcat tataggcatc gatgcttttt
    39601 gctttcaaat cattgaggac cttgtaacgt ctttccattt cttccaccgt ccagcgcaac
    39661 acctgcgacg cctttttcgg gtccgtcacc accggatgca tcaggtgcga aataccttcg
    39721 tacgaggata gctccagccg tttgggatcg atcatcagaa attttacatc ttccggttgg
    39781 gccttgaaaa ggatgctgca gatcatggcg ttaagcgcga cgcttttccc ggagccggtg
    39841 gtcccggcga tgagcagatg aggcatcttg gtcaggtcgg caatcacggg cgtcccgacg
    39901 aaattaacgc ccagcgcgat cggcagccgg aaagatgatt gcgtgaagga atcgttgtcg
    39961 atgacctctt tgaggaaaac agattctctt tttaaatttg gaatctcaat accgatgacc
    40021 gatttgcccg gaatcggggc cagaatgcgg atgctcggag ccatcagcgc cagcgccaga
    40081 tcgtctgcga ggttgacgat cttgttgatt ttcactcccg gagcgggttc cagctcatac
    40141 atggtgatga ccggtccggg cgtgacttcc accacccgtc cctcgacgcc gaaatcggac
    40201 agtttctttt ccagaatccg ggaattggtg accagcgcat cgcgtttgac gcgggcatcc
    40261 ttttgcgggg cctcctccag cagggtgaat ggcggcaact gaaatttatc gtcgcttttt
    40321 accttcgtaa aatcgaaatg tgtctgttca actttcttgg ccggcgcttt tttggtcacg
    40381 tccggcgctt tttcgacaat agcgggttgc ggcggggacg ggggttcgga tttcatgcgg
    40441 ttgaagacga aatcgacaaa ggccatgatg cgctctttcc atcttaacaa aaaggcggcg
    40501 ccggatttgg aaattttttc cgtgaacgag atcatcgaaa aatcgaccag gaaagtcagt
    40561 gacacgacga aaatcaaaaa taaaataata taggttcccg ccacattgaa atagttgcgc
    40621 agcaaaccga caatggcgac gcctgtgagg cctccggtac cgtttttcaa cgattctccg
    40681 taaagggaga tgcttccgct gatcagaagc gccatgatgc cggcgaagga aaagatgaaa
    40741 aacagaaagc cgattaaacg gccggtgccg atgcgaaagt cggaagaccg aaaatattta
    40801 aaactgcaaa cgaacagcat cagagggaaa agcaacgaag cggcgcccag caggcggatc
    40861 agtgaatctg cggtatacga tccggccttg ccggtaaaat tatgcgtggt gtgatcgccc
    40921 gcgacaaaat gtgtgaaaga ggggtcctgc gggtgatagg aaaccaggca caggagcaaa
    40981 aagaggccca gcgccagaca aaccgcgcct ttgatttctc ttgttctttt attgtccgtc
    41041 tgtttttttt ccatgttgtg gccgaatcaa taatagtaat accgatatta tttcagaaaa
    41101 gagaagattt tacaagcagc gtaaacgttc cgaagaacca gccataatag gcaaagtatt
    41161 ttaaacgcgc ctcccggata atcaggaaaa acagtttcag ggcgaggagt ccggccaatg
    41221 ccgcacaaac gaaccctgcg aagtagagat atgatcccgg ggaggaaatc tgccgtagat
    41281 agccggcttt cagaatcacc gccccgcata ccgccggcag ggagagcaga aaagaaaagc
    41341 gtgccgccgc gttccggttc agattgcaga aaattccgga cgtgatcgtt gtccccgagc
    41401 gggaaatgcc cgtaagaaaa gggacggcac gggcgcttcc ggagaggata ccgtcgatga
    41461 cggtcatatt tttgatatcc cgtcgcgtct tcttcatctt gtctgctaag aacaaaagaa
    41521 tgccggtgat gattaagaaa aatgccgctg cccgggctga ttcaaagatg ttgtgcatct
    41581 ggtctttgaa cagatacccg ataacggcaa ccggtattgc ggcgataatc aataaaagca
    41641 ccgcgtcgaa ggccccgccc ggctgatgaa atcccgggaa aaagctctgt acaataacca
    41701 ggtgctcaaa cctgctgaca ggaaacagtt cggtaaattc ctgaacgatg ccgggaacga
    41761 taacggtaag cagattcaat gtgccccgct ttccagatct ttcagaggan nnnnnnnnnn
    41821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aacgataacg gtaagcagat tcaatgtgcc
    41941 ccgctttcca gatctttcag aggagacatt tcaatatcct gccaggcatt gtaatttttt
    42001 attatttcat ttctgacttt ttctatcagt gcgtgtctgt tttcaatcgt gacgtcctga
    42061 acgtcgatgg gttttccaat caccattttc aactgaccgg gttttatccg gagggaccgc
    42121 ttgggcatga tccggccgct gccgataatg gaaatcggaa tgatcggcac tcccgatttg
    42181 acggccagat gaaaggcgcc ctgtttgaac gctttgattt ctccttcgcg gcttctcgtg
    42241 ccttccggaa aggtcatgac ggacttgccg ctccggatgc gcagtgcggc ttcatcaatg
    42301 ctttgcattg ctttttcgcg gttgctccgg tcgatttcaa tatatccggc gctgcgcatg
    42361 gctgcgccga ataccggaat ggcgaacaat tccttttttg cgatccagcg gaattgcacc
    42421 ggaatatgcg ccagtgaaat taaaatatca aaatcgcttt gatgattggc cataaaaatc
    42481 tgcggttttc cgaacagaat gttttcttta ccgatgacat cgacttttgt gttgcagatc
    42541 atcagtaata gctttgccca caatgttgcc accttgtgaa ttttattgtc agcggcggga
    42601 aagacggaaa acaccaccga ccagaacgac atgaacgccg ttatggcaac acctaatgtg
    42661 accagtaacg ctgaatggac cataaacgtt ttcctgctca gacggattaa tgaataaatt
    42721 tttacttgat aataatagtg aagtcaatca tgaaaaaagt ttcaagaatg atgaagtcgt
    42781 cttcaaacgt atcggaggta gagaataaaa aaatcagggc gttcagattt taaaattctt
    42841 gttcctgaac agtttcaggc aggctgacac cgcttcaacg tcatagagcg taccggcatt
    42901 gttttcgatt tcagccagag cgatctctat gcccagcgcc ggccggtacg gacggtagga
    42961 aatgatcgct tccacgacat cggcgacggc aataatgcgc gcttcaatca gaatatcctc
    43021 gcctttgaga ctccggggat acccggaacc gtccatccgt tcatgatgct ggtatacaat
    43081 gtcggcaagc ggccaggggg attcgacgtc tttgatgatt tcgtaactgt actggcaatg
    43141 cgattttatc aaagaaaatt ccagttcgct caacgtggtt ggtttgcata atatttccga
    43201 cgggatggaa atcttaccga tatcgtggat cgctccggcc atgcggattc cttcgatggt
    43261 atcttgcggc agtttcatct ccgtcgcgat agcgcgggcc agatcggcca ctctccgttg
    43321 atgtccggcg gtatacggat ctcttgcttc cgaagcgatg cccagcacct gaatggtcgt
    43381 attaatggat ttgcgcagcg tatgaagggt ctcctgcagt ttcaactcgg cctttcttcg
    43441 ttcggtaatg tcttgataaa tgatttgata tcgtttttgc ccgttccaga atatttcctt
    43501 gcgcagtgca aggacatgcc gcacttcacc gtcttttctg acaatgctga tttcgtattc
    43561 gggcggaccg gcttccccca ttaaccgctt ttcctgccgc tgttgaaaat cggcgtaact
    43621 ctctgcggtg taacgtttgt gcagaggcgt gttttttaat tcatccacat aagtgtaacc
    43681 gtaaatatcg agaatggctt gattggcata gagcgtttcg ccttcctccg tgacaatgcg
    43741 tacgcccaga ggagaatcat caagggaatg acggaaattt tcctcgcttt ttcgcagggc
    43801 atcttccatt tgtttccgtt cggtaatgtc gtgaataatt ccgcggaagc cgatcggctt
    43861 tccggacgaa tcctttttca gggacgccga ctgttcgatg tagcgctttg tcccatcctt
    43921 ccggatgatt tcccagtcga attcttttgt ggggcgtccc gttctataga ccttgttaaa
    43981 ggcctggaaa acttttcttg cggtttcccg gctggtatat tgacggttgt tcattcccct
    44041 taattcgttt tcggaataac ccagcagtgc acagaccggg tcgttaaaaa aagtgaaatt
    44101 gccggcaaga tctatttcat aatacccttc ctcggtggtt tccagaatcg tccggtattt
    44161 ttcttcgttt tcgcgttgtg cctgctgtgc cagattccgg gatgtaatat ccgtaataac
    44221 aaccaggaca tatcgttttt cgcgatgttg gatgatttcc gcggaaaaaa gaccgtcaag
    44281 gatcgtcttg tcccttcgcc tgaacttcat ttcgaaattt tcaatataac cctgctgcat
    44341 cagttgtttc agtgcgatct gtcgtttgtc ctgttcgaca aaaatatcaa gttcgtcgct
    44401 ggtttttcct atgacgtcct gccgggaaaa accgaacgtc cgcaaaacgg ttttattgat
    44461 gtcgatgtat tttttttcgg gaagtcttaa cagaacaatg gaagcagggt tgtgctgaaa
    44521 aaggaaaagg gatttctgga gccagttttc atgcttttct tccggttggg gtcggtaaag
    44581 gaaggcttct tttgtgtctt ttctgctttt tactggaggt gttttttttg catttgccgt
    44641 ttttatttcc tgcattcgtc catccgtctc gagtcctttg tggattttcg aagatgttgc
    44701 gcaacaatgc cggaatgaaa atgaataagt atgataaaat ataaaataat ttattgcgtc
    44761 tgtcaaatat taaatgcccg gcggattttt tattccaggg acatcaggga gttccagcgt
    44821 tcctgctcgg tggcgtgagg cagattgttt ttgcgaagaa gatggatgat cgattcttca
    44881 ttaaaccgca ggtacgggtt aatgccgcgc tcttcatcca gggtggaata ggttgtgttc
    44941 ctggagcggc aggcattccg gaaaagatcg atatttttat tccggggttc cagatgacgg
    45001 gcgaacgcca gagcattcag gatatagtcg tggccggcat aaatcatcgt ttcaccggac
    45061 aggtccatca gccgtttgat ggaaaggaag aaaatattca tgttgccgga aaaacagttg
    45121 cccacggttc cgttgaaaag cgtgtccccg gtaatgagaa aactcccggc atgaaagcag
    45181 acggaatcgt ctgtatgtcc gggagttcgg tagaccagga tgcgttctgc gttgataaca
    45241 agaggctgat ggtcctggaa atcagccgcg cgaatgaatt cggcgcctgt tttttgcagc
    45301 aaatggtcgt ttcccggggt atggtcgtag tgcgaatgcg tgttggtgac gattttcagc
    45361 atgagattgt tttgcttcag aaaatcaagt atttctttcc atgcgccccc gtcgacggcc
    45421 acggcttgcg tttttccgta cagaaggtat gagaaattgt ccgttccata ccgtaactgt
    45481 ttaatattca acatggtctt atcccattcg attcgccgaa taacggcttt tcgggtttcg
    45541 tttttctccc gcaggcatat taatgaagat tccggggaca tgtcaagata atcataaata
    45601 gccgacgtcc tgttttttag aaaatgtttg acaaattcag atcttaataa taatatcacg
    45661 caactttgta aacgtacggg cgcgtgaagc gcttttttta attttcagca tgatggtttc
    45721 gggagttgcc ttcaaacgtg caaaaattac tgacaaaaaa gagagcgttg gaaatcatcg
    45781 ccggtttccc ccgcgcgggc gttctggtga tcggcgatgt catggtcgat catttcatct
    45841 gggggaatgt gtcgcgtatt tcgccggaag ctccggtccc cgtcgttgac gttcagaaag
    45901 acaacatttt gctcggcggt tgcacgaatg tgctcaacaa catccattcc atgggaggcc
    45961 gggcgtacgt ttcgggagtt gtcggggccg atacgatcgg caaacagctg ctggccgagt
    46021 tgaatgatcg taagattgaa acgaaaggca ttgtggttaa aaaagaccgt ccgacgacat
    46081 tgaaaacgcg aatcgtggct cacggacagc agatggtgcg gtttgacaag gaagacagga
    46141 aaccgatttc tccggtaagc atccacaaga ttctggacta tgttcggtcc cgggtcgata
    46201 aaataggagc gatcgtcatt tccgattaca ataaaggtgt tgtctcgaag gaactcattc
    46261 agggcataaa aaaaattgtt gatcattcgg ggatttnnnn nnnnnnnnnn nnnnnnnnnn
    46321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46381 nnnnnnnnnn nnnnnntgtc tggcgaatct ggctgccggc attgtggtcg agaaagtcgg
    46441 aacggccacc gtttcgcgac aggagctgat cgacgtatta tgagtaaacc gagtccggcc
    46501 gaaccggtga aattattttt cagccttttt gcgcaggatg ccgtaatcat agacgagacg
    46561 ataaaagagc tttcccgggc ttacggggac ccggatttta taagtgcgct tcagccgttt
    46621 gattacacgg attactacgg cggagaaatg ggcgaaaatc tgattcgccg gtatctgacc
    46681 atggaaaaat taatccatcc ggaatccctg ccggatatca aactggccac caatgacatt
    46741 gaaataaaat cggcagccgg tttgaagaga aggataaaca ttgatccggg ttacatatcc
    46801 aaggctcatc tgattttagc gaccggcaag agttacaccc accgtccata tttgagagac
    46861 ggaatttatg ccgatttgac gcttgtttat caggggaaaa aattctgcgt tctgccctgg
    46921 acctatccgg attatgccga tgcaaaacag ctggagatgc tggaccggat cagaaacaga
    46981 tatttgacgc agttgaaaat gacggatcat taaaaatagt gaacaacaat cgaacaaaga
    47041 caggtttatt gaaatgatta aaagcatgac cggctacgga cgggcggaaa aggtttttca
    47101 ggacaaaaat atcattgttg aagccaagtc cgtgaaccat cgttttctgg aaatcgtgct
    47161 gcgcacgccg tcggcacttt ttccgatgga gatggactat aaaaagaaaa tcaccgaaag
    47221 attcaagcgc ggacgcattg aagtattcat caagttcgaa ggcgaaggtg ccgacacagc
    47281 gaaagtgaat ctgaatatgg aaatagcgcg agggtatttt aatgtattaa acagacttcg
    47341 ggaagaattc gatctggagt cccccgtgac gctaaaaaca ttgaccgcct ttcgcgatat
    47401 ttttacgccg cccgcggaaa ctgaaatcgg ctctgatttt ctctgcgagg tggaaaaatc
    47461 gtttctcgag gccttggaaa ttctggccgc gatgcggcag ggggaagggc ttgtcctgtt
    47521 gcaggatatg caaatgaggc tgaaaatgat agacggtgtt cttaatgata ttgccgtgcg
    47581 tgcgccgcag gtcgtttcgg aatatcagaa acgcctctcg gaacgcatta aagaactgac
    47641 cgaagggttt gcgcttgatg aaaaccgttt ggctcaggaa gtggccatca tggcggaaag
    47701 gaccgacatt acggaagaaa tcgttcgcct gcgaagtcat ctgggccaat ttgaagttct
    47761 tctgcacagc gatgaatccg agggcagaaa aatcgatttt ctgctgcagg aaatgaaccg
    47821 cgagatcaat accatcggat ccaaggtcgg cgatgtcgac atcacgcgtc atgtcatcga
    47881 cgtgaaaagc gaactgggaa aattacgcga acaggcgcaa aatatagagt gaggctatgt
    47941 ccaaaggatt attcatcgtc gtttccgcgc cgtcaggcac ggggaagagc agcatctgtc
    48001 agggctttat ggaagcgttt ccggaaataa aattttcggt ttcgtatacg tcgagaccgc
    48061 cgcgtcccgg tgaaatagac ggaaaggatt ataattttat ttctcgtgag gcgtttcgga
    48121 aaaaaatcag gcagggcgag ttcattgagt gggtggaaaa ttacggccac ctgtacggat
    48181 cgtcgcggcg cttcatagaa gaaggtgtgc gtgacggcag ggatctgctg ctggatatcg
    48241 aaccgcgggg cgccaaaaaa ataaaacagg aattccggag aggcatttat gtttttattc
    48301 ttccgccgtc ccgtcaggaa ctattgaaaa ggctgaaaaa acgaggtcat gagcgtgaaa
    48361 acgttattca aaaaagatat aagcagtcgg aaagtgaaat aaaagagatt tcctggtatg
    48421 attacgtcat ctttaacaat gatctgaaaa aggccgtcaa tgaattgatt gccatttata
    48481 cggcggaaaa gtgcaagagg acaagattaa acagagaaat aagagaattt ataaaataaa
    48541 tatatacttg aaggagatgt aaacgctatg gcgagaatta ccattgaaga ttcgttgcga
    48601 gtggccaaaa cccggttcgg tctggtgtcc ctggcctcca aacgggcgca tcagctgctg
    48661 aagagttcgc agcctctggt cgtgagtaaa aacagagaaa tcgttcaggc gctgcgcgaa
    48721 attgctgcag gcaaggttgt ttatgccaat cccgaatatc tgaacggaac caaagaggat
    48781 ttcaaaccaa ttccggacag cacagagttt atcggcgatg aagaatatat cgagtaagaa
    48841 tgcaggcgag agattaatct tcgcgcttga tgttcagaat tatgaagatg cgttatcgct
    48901 ggtggacctg ttgtccggcc atgtcggaat gttcaagatc ggcaaagaac ttttcacagc
    48961 cgccgggccg aaaatcatcc gggcgatcaa ggaaagaaat caaaaagtat ttttagatct
    49021 gaagtttcac gatattccta acaccgttgc ccgtgctgcc gaagccgccg tcaaaatgca
    49081 tgtggacatg ttcaacgttc acgcgtcggg cggttcccgg atgatccgag agtcggtttc
    49141 cgccgcatgg acgtgttccg atgcactcgg tcgtgccagg ccgatcctgc tggcggtgac
    49201 ggtgcttacc agtctgaata acgacgattt gaaagaaatc ggctttcaaa aaacaacaaa
    49261 cgaactggtt gttcacctgg ccgggttggc gaaggcggcc ggagcatccg gcgtggtggc
    49321 ttcagcgcag gatattgtca tgttgaggaa ggccttcggc gaggattttg tcattgtcac
    49381 gccgggcatt cgcagtgcgg ccgaaatccg aaaagacgac gatcagaaaa gaacgctcag
    49441 cgcgtatgac gctgtggcag ccggcgccga ttatattgtc gtcggcaggc ctattcgcac
    49501 ggcagccgat cccttgaaag cctgccgcca gattgtccag gaaattgccg aagggctgtc
    49561 ggcacggtaa tgcctttccg atcgcggaag ataccatgga gttcatttac ggcataaatt
    49621 ccatcagaga tctcctccgg ctgcagaaaa gcggatggga aaaaatcatg attgcctccg
    49681 gaaggaccgg ccccgctgtg caggacatta tcgctatggc ggaagctcac aacattcccg
    49741 tggaattttg tgaacggaaa aaactggata aactggccgg aacacccaac aatcagggca
    49801 tgatcgcttt tcgtcaaaca tttgcttacg cggatctgga aactgtgatg aaaaatcgtc
    49861 accccgcgtg tggtcacgat ctgattctca ttctggacag cgtcatggat cctcaaaatc
    49921 tcggttcgat aatccggacg gctcattgct ggggggccaa cggcgtgatt attccggaag
    49981 acagatcggc aggggtgacg gcggccgtga acaaagcctc cgccgggagc gttcatcata
    50041 tccccgtcgc caaagttgtc aacctggctc aattgctgga tcacctgaaa gatcgggggt
    50101 tctggatttt cggtgcggag acgcacggcg ggaagaaatt gacggaaacc gactttgact
    50161 gtcccgtcgc tctggtgatg ggaggcgaag caaaaggcat caggcctctc ttaaagaaaa
    50221 aatgcgactt tttgaccacg atcccgctga ccggtaattt tgattcgttg aacgtgtctg
    50281 ttgccgcagg aattattttg tatgaaatat cccgtcagcg aacccggtca actaaacaaa
    50341 gcggcaaatg aattgatcgt caccgtcttt ttgatgcgtg catcgtgcgc agggaataag
    50401 cgattttgga aaatggcctg ccggttcgac gaaagacggt tatttaactt gttgaatttg
    50461 taaaaaatct ggtttaaaat gcgaaaactt taagttatcc gattggggga aaggactaaa
    50521 tatgcctgta tttatctggg aagcggtaac gaaaaaagat gaagccaaaa aaggagaaat
    50581 ggaagccgta gatgaaatta ccgttcgcag tcttttacgc cgccagggtt acaaatccat
    50641 tgaagtcaag agaaaaccca aagatcttct tgaatacctg ccctttttac aggggaaggt
    50701 taaggaaaaa aacgtcgtta tcttctgccg tattttttcc acgatgatca acgccggtct
    50761 tccgctgata caatgtctcg atcttctcgc ccagcaggag caaaacaagg ccttctccaa
    50821 aattatcaag tcgatcaaag aagacatcga gggcggaacg agtttaacga acgcgttgaa
    50881 aaaatatccc gaaatcttcg atgaactttt tgtcaacctc attgccgcag gtgaagcggg
    50941 cggtattctc gatatcattc tgcaaagact ttccagctac atggaaaagg cgatgaaact
    51001 gaaagcgcgt gtcaagggtg cgatgacgta tccggccgcc gttctggtga tctcctgtgc
    51061 ggtcgtggcc ctgctgctga tcaaggttat tcccgttttt caaaaaatgt ttgagggaat
    51121 gggcggggaa ttgccggcat tgaccgccgc gctgattgct gcgagtcagt ttgcacaaag
    51181 ttactggtac cttggcgttg ccattctggt agtaatttac attattcttt cgaggtttta
    51241 tagaacggaa aagggacgtt ggacgattga ttcgctgatt ctcaagtcgc cggttttcgg
    51301 gccgctcttg aaaaaagttg ccgtggccaa atttgcgcgc acgctgtcta cgatgatgag
    51361 ctccggtgtg cccattctgg aaggtttgtc catcgtcagc aaaacgtccg ggaatgtcgt
    51421 tattgaagat gcgctcatga aaacacgcca gggcatcagc gaaggtcagt ccattgccga
    51481 accgctgacc aaaacgggaa ttttccctcc catggttgtg caaatgattg cggtcggtga
    51541 agcgacaggc gcgctggatt caatgctgtc caaaattgcc gatttctatg acgatgaggt
    51601 cgatgctgcg gtggatgcca tgacggcgct tctggaaccg gtgatgatgg tttttcttgg
    51661 aggcgttgtc ggcggcatga ttgtggcgat gtatctgccg atcttcaaac ttgcatccgt
    51721 tgtcggttga tgcgaagctc ctgcctcaca ggatgccatc gcgggcatgg tgcctgtcgt
    51781 tttccacaga gggcgcctga cggtttgcgg ttggagacga tcctgtgagc tttgccggcg
    51841 ggattgtttc gcaaacgttt cagagaccgc gtcgcctata gcgaccccat ggtgccctgg
    51901 agggtattca ggtcattcac tttcgggata attcgatcaa cacatttttg agtacgccta
    51961 tcgtgccctt tgggctattg aggggatacg cttttgaaat cgggacttca taaaaatggt
    52021 cgggatggcg cgatttgaac gcgcgactcc tgcgtcccga acgc
//
LOCUS       DAONQZ010000010        50554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_733_length_50554_cov_16.999228, whole genome shotgun sequence.
ACCESSION   DAONQZ010000010 DAONQZ010000000
VERSION     DAONQZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 50554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50554
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_733_length_50554_cov_16.999228"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..427)
                     /locus_tag="PLR13_03295"
     CDS             complement(182..427)
                     /locus_tag="PLR13_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18388.1"
                     /translation="MNKGISGASLGVGGDIWTVDNPVPFKFSELLLPEIIIEDYGTEQ
                     DYQRVMRPAFDTLWNAAGYSESKYFNKNNLWVGRSKR"
     gene            complement(628..1797)
                     /locus_tag="PLR13_03300"
     CDS             complement(628..1797)
                     /locus_tag="PLR13_03300"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HQC18389.1"
                     /translation="MKTADKLAKIPPYLFMELRKKINKAKAEGIDVISLAIGDPVEAT
                     PDSIIDELCATSRDPLNHRYPTDEEKGMLAYRREIAKWYETRFGVTLNPENEILGLIG
                     SKEGCHHFIIARVNPDDIVLMTDPGYPAYRSSILLGGGVPYNVPILPQNNYLPVFEDI
                     PTDIAKKATAMFLNYPNNPTGACATLEFLDKLVEFARTYDIAVCYDNPYSEIVFSGQK
                     RISFLMAKGAKDVGIELNSLSKPFNMCGWRLGMACGNPELIAAISKVKENTDSGIFNP
                     IQFAGIKALQNEAPFVDKMIELYGKRRELVLKTLKKIGIEFNPPRGTFYLWVPVPKGM
                     TSLEFTNRLFDKTAVVVAAGTAYGQYGEGFVRISLTVPDERLREAMERIEKEFKQ"
     gene            complement(1976..2794)
                     /gene="dapF"
                     /locus_tag="PLR13_03305"
     CDS             complement(1976..2794)
                     /gene="dapF"
                     /locus_tag="PLR13_03305"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQC18390.1"
                     /translation="MIEFYKMSGSGNDFIIIDNMDLSLDVGDLSEFARKVCQRKISVG
                     ADGLVVIEPSDIADFKWRFFNADGSMAEMCGNAARCVARFAYMKGIAGQTMSWETIAG
                     IISAVVNDDVVKAKLTDPSPVESNIEIETDGLKFILDSIDTGVPHAVVFVDDLDTCEV
                     YRQGRNIRFHEHFAPRGTNADFVSLINRNKIRVRTYERGVEDETLACGTGVVAAVLAA
                     AQRNLVESPVDVLVRSSESLRVYFENQNGLCREIYLEGRVKMVYQGMLLEEAYQ"
     gene            complement(2891..4684)
                     /gene="gspE"
                     /locus_tag="PLR13_03310"
     CDS             complement(2891..4684)
                     /gene="gspE"
                     /locus_tag="PLR13_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HQC18391.1"
                     /translation="MSKIHTALKESAASAHGDASSGKSVFSSLDEKLIAAGVAPDTLS
                     EIRTAPANNGGGFFDQLIKKKVIPEIHLLKILSEHFGLSFWQQLPMESIDIDFTQRIP
                     IQYLKKHKIIPLITAENPVIALNDPSNFQPVDDLRQLLKNPNLSVVLSLQDAIMSAIN
                     MAYDMSRSSAKDYFEEMDQSSTDDLISKIDETADLLDEASDAPIIKLVNLILSGAIKD
                     RASDIHVEPYSANLKIRYRIDGILYDTLSLPRRIQSPLISRIKIMAKLNIAEKRLPQD
                     GRIEIKIADRLVDIRVSVIPTAFGERVVLRLLDKTANILMLSDLGMHDERIKLLNKLI
                     KSPYGIILVTGPTGSGKTTTLYAALSTINRPEINIITIEDPIEYQMDGVGQIQVNPKI
                     DLTFAAGLRSIVRQDPDVILIGEIRDRETAEIAIQSSLTGHLVFSTLHTNDAASAVTR
                     LIDMGIEPFLVTSSIVAIIAQRLVRVLCPHCKEVYEPDEESLANLGLSRSVLKNNTFY
                     RKKGCNLCMQTGFRGRSAIFEILTVDDEIKRLVLKTSDANQINELAIKRGMITLQQDG
                     IDKVLAGITTIEEVLRVTRTLSRTDDIVIEV"
     gene            complement(4705..6936)
                     /gene="gspD"
                     /locus_tag="PLR13_03315"
     CDS             complement(4705..6936)
                     /gene="gspD"
                     /locus_tag="PLR13_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HQC18392.1"
                     /translation="MKKSHVLILKAGFLIWVMILLVIISGFAGSALAARVAGGGEEIE
                     TTAMPDAAPEPAIEEEVDPAPEPELMNEPSRQNAEMDAPVMSTDNKDEKRSYKPIKVN
                     PSQKYVTLDFDNVDVSVLVKFVSELTGKNFIIDDKVKGKVTIVSPKKIPVEDVYKVFL
                     SVLEINGLTVVPSGNMIKIIPSVQAREKGLETRTKQDPLLPEDRMVTQIIPLERANPD
                     EVKRILDPLIPKSSSVLSYPPAGMLIITDYLSNIKRIQEIATALDVEGAGDQISYIPL
                     QNASASEVVKSLNTIFTQGPVRGKAATILKVVADSRTNSIILLASGAETENVRKLIAF
                     MDKDVPRGESNIQVYRLQNSVAEDLAKVLTSIVKEGGAAAGATATATGAKTAPTTVVS
                     KNVQIVPDKATNTLVVMAEREDYKIIENIIKQLDIPRPMVYIEALIMEVNANKDFKLG
                     VEWRGITEHGSVDGNPTAAFAGSGGAGPSGPGSYNIMDGLLTTTGMGVFPSGFSMGLV
                     GAGIKIGGVTFPSIGAVVQAYKTDSEVSILSTPQIMTLDNEEAEINVGSNVPYVTRQD
                     QTNVGTGTNYTNYSSYEYRDVGVILKITPNINQENFIRLKIDQQVTKVQSGADEARPT
                     TLKRTAKTTVIVKDNETVVIGGLVGDSTDDSTYKFPLLGDIPLLGWLFKTKTSSREKT
                     NLYVFLTPHIVRTQADAAKIYQEKRETMGEVVEGVIKLNEKADAKAPQVRPPVENETI
                     SNP"
     gene            complement(6944..7723)
                     /locus_tag="PLR13_03320"
     CDS             complement(6944..7723)
                     /locus_tag="PLR13_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18393.1"
                     /translation="MSYINDALRKAQQDKKSPYAAYEPLLSPSGKKNTGRRRWLALLG
                     FLALFFCVAVAGLFVLMSGPETKKATAKAPLTIPTAPVIETPVIEAPVQPAVQDIKVE
                     NKVEPVLKTTEKKTQKTEESAALKIRTADSVKLYAEALRKHQEGNLEQAKDLYKKVIE
                     IDPKNIEALNNLGVVYMKKKTYKWAIIRLNDALKIKHDYPDAHYNLACIYAQNNDLSR
                     SLSYLKNAIGFNPQVRQWAEKDKDLEVLADLPEFKKLLEKN"
     gene            complement(7757..8602)
                     /locus_tag="PLR13_03325"
     CDS             complement(7757..8602)
                     /locus_tag="PLR13_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18394.1"
                     /translation="MKENPFSLSPEPRYLFLSEQHRDALNCLIYGIKEKKGFVLLSGD
                     IGMGKTTVCRSLLASLDDSVETALIFNTAVSDIDLLETILEEYGVDIERESRSKKNYI
                     DRLNSFLLENFSAGKTAVLLIDEAQNLSHAVLEQIRMLSNLETETEKLIQIILIGQPE
                     INNHLMLPALRQLNERITVRYALKPLSPPEVREYIHHRLKVAHGPGDIRFTGAAYRLI
                     FNFSEGIPRRINTICDRALLIAYTKSITKIDRKIVRLAVHDIGTTYFQKTMSLGRKLL
                     ARLTV"
     gene            complement(8646..9497)
                     /locus_tag="PLR13_03330"
     CDS             complement(8646..9497)
                     /locus_tag="PLR13_03330"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQC18395.1"
                     /translation="MDTGVLISVAVVLVITILSYEATDLFYKVVRFPLTRHTAVIKNH
                     HESFVRNNRQHQQLQDYDIITERNLFLTTLRTAGGSESADGPFDADQKSMDFDLKGTI
                     AISSSSGFIIVEERGSKKQKLYRLGDKIGARKLAKITRNTAVLKNGEEEITVKIKETV
                     EDNLLSPAPGSRRNPSPSRNPALSRLGIDGNAKYLQSIMNQAVVRPFMSRGVRQGYII
                     SNIKPSSLYEKAGLQNGDIIVGINNNPLHNANDVMQVLNAMQSGSRLELTVKRRGRTE
                     TINYTLE"
     gene            complement(9578..10396)
                     /gene="gspN"
                     /locus_tag="PLR13_03335"
     CDS             complement(9578..10396)
                     /gene="gspN"
                     /locus_tag="PLR13_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HQC18396.1"
                     /translation="MKILKNKIFWFTIYGIFITLVFLYLLFPSDIAQSRLEESIASSG
                     LILKTGSLKPSLPLGLKMKNVRLGSGSSPNIYFQGDSLDVQFHPLNFFRDKKNIRLSG
                     KAYGGTFSGNFKTTAAGFHPPEEGMLKFKNIALDRYTLIKALTGKQITGKASGNWTQA
                     AGSGGRENSSATISLFLNKGSYPLAEPFLGLQRIDFDRGEIQARLENRVLKFEKFQIF
                     SPQMDCSLNGDIMLVDEFKNSPVNLTGEIIISGRKVKMNITIEGTIANPSIRYM"
     gene            complement(10393..10959)
                     /gene="gspM"
                     /locus_tag="PLR13_03340"
     CDS             complement(10393..10959)
                     /gene="gspM"
                     /locus_tag="PLR13_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HQC18397.1"
                     /translation="MIQELWNKLDATQRKLVAGAAVFVCIALILELAVFPFWEAKKKL
                     DRSISSNQKKLAEVKELSAEFNRLESKVSAMRRVASMRAANFTLFSHLEKKATQAGVR
                     NRIKNMNSSRGIQSASFEESLIDMKLEKVTIKQLVDFLYYAESPADLVRVKKIHIVKM
                     KDAPEYLSVQLQISSIQPSNQIPESGQP"
     gene            complement(10956..12470)
                     /gene="pilM"
                     /locus_tag="PLR13_03345"
     CDS             complement(10956..12470)
                     /gene="pilM"
                     /locus_tag="PLR13_03345"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQC18398.1"
                     /translation="MPNVLGLEIGRSTIKAVLMARKGLGGGRILDARILDVRDSEGIG
                     PALQKLAEDKNFAGIPCFVSVPSQDVLFRHVRLPFRDDNRIRKTLLFELEPLIPLPIE
                     NVIADYIALPREGLLVGAMPKETIREIIEQVEKNLGDVSVIDTSSSALAVQIPDHKKT
                     SVSIILDIGRNATTAAFCENESVIHIRSLAFSGEQITLAMAKDFSVDRNRAEQLKISG
                     DYPEAMEKDGEECRGLCSELKNTVEFMKINGVLHNDPAQILLTGGGALFGPLRKEIEK
                     NFTCPVELLNLISARQLEVEKNIMMRMNPSLLNAAAANALRSSAGKKSFNFRQGEFTA
                     RPSHLNFKNHLRWAGILAAVILLLAVVNQILDYSLKMRQIGGIKKDIARIFQKSFPDA
                     PSMVDPVQQLKTKIEEDKKTFGLGEGMPEATTADFLKELSALISPSLEIVFTALTYEN
                     RVISVKGEAKTVDDVTLIKDALAKSKSFKEVTMGPTSLTRDGGKVDFSLRIEVQ"
     gene            complement(12470..>13425)
                     /gene="gspK"
                     /locus_tag="PLR13_03350"
     CDS             complement(12470..>13425)
                     /gene="gspK"
                     /locus_tag="PLR13_03350"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HQC18399.1"
                     /translation="NPRGVALITVILIIVILIAVVIELNRSSRADIYDAANLSDGIKL
                     SYIAKSGFYGAAALLNNSPGAYTSLRDDWAKMETVSLQSNTLFPEGYFIARIEDEAGK
                     IPLNKLVDGEEYNEEIRTMLDRLLAQPEFGLKDRQIDEIIDAIKDWIDTDENTTGYGA
                     ESNYYESLDSPYQAKNAPFDCIEELLMVKGITKEIFYGTKDKPSLAKYVTADSEGLIN
                     INTAPKIVLRSLSDDISSELADRLDEYRMEKSHDVSGNQWYKQVPGMENITINQNLIT
                     VKSTYFKIVSTGKISNMSRTVTGIVATGDQAQIAIITWRQD"
     assembly_gap    13426..13525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13526..13760)
                     /locus_tag="PLR13_03355"
     CDS             complement(<13526..13760)
                     /locus_tag="PLR13_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18400.1"
                     /translation="MMNIFARHQDDQKKNDPQKSFLKFSILKGFMAFRKRRDGSTYQF
                     PFTAFRKLLFHNPLLSRDGSTYQFPFTAFRKLLF"
     gene            complement(13762..14430)
                     /locus_tag="PLR13_03360"
     CDS             complement(13762..14430)
                     /locus_tag="PLR13_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQC18401.1"
                     /translation="MRHCSSRPHLSGGGFTLVEILIAIFILSLVLATVYVSYTGTLKT
                     SHQLEKEGEIYKMARQSLERLAKDLTSLQMSEGSFYLNAEKKKTGRHQFHNIFFWSAA
                     HLTFGELEGEGRPATIGYYVTENPDGKSFSLWRSDATGMKPDETKKTEDGFIICKNIE
                     TFHLTFYDSAGGENDTWETSAGGGMHQGKVPEAVKIELALVNDDDAQKPYKFMTRVFL
                     PVNK"
     gene            complement(14433..14804)
                     /gene="gspI"
                     /locus_tag="PLR13_03365"
     CDS             complement(14433..14804)
                     /gene="gspI"
                     /locus_tag="PLR13_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HQC18402.1"
                     /translation="MTRMTEKGFTLMEVMVAMAILAIALVSIFQLQSQSISMATDSRF
                     MTTAALLAQSKMVEVETQSPLSNKTENGDFGPDYPQYAWQLIIGDTQLPQFKKIEVTV
                     TNKLFLRRGTYTLILYKRPDM"
     gene            complement(14815..15366)
                     /locus_tag="PLR13_03370"
     CDS             complement(14815..15366)
                     /locus_tag="PLR13_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQC18403.1"
                     /translation="MSIRTSTTGKWSKQGFTLVELLLVITLIGIVLVLAVPSTRDVLT
                     GDMLKKTSRQLIGLERKLRTEAVRDQLDYILHLDLSDSVYWITASDMTEIKQAEIKKQ
                     ALHLPEDVRIIDVVDEDNRKITAGEVKIVFKKDNICSPRVIHLAYKEDKMTIVMNPFM
                     GVTDIYDQYVEIPFNTSEGQNRR"
     gene            complement(15329..15781)
                     /gene="gspG"
                     /locus_tag="PLR13_03375"
     CDS             complement(15329..15781)
                     /gene="gspG"
                     /locus_tag="PLR13_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQC18404.1"
                     /translation="MKIFRRKKSRQAGFTLIELMVVIVILGVLAGMIIPRIMGRPDEA
                     RQAKARIQMEAMESALKLYKLDNGSYPSTEQGLKALVEAPTTGDLPKNWRQGGYLEKG
                     KVPKDPWGNDFVYLSPGSHGDFDLSCLGKDGEAGGEGVDQDINNWEVE"
     gene            complement(15810..17036)
                     /gene="gspF"
                     /locus_tag="PLR13_03380"
     CDS             complement(15810..17036)
                     /gene="gspF"
                     /locus_tag="PLR13_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HQC18405.1"
                     /translation="MSVFEYIALDEKGRQLKGFIDASGISAARQKLREENIFPVEINQ
                     TETKKESALAGALKINLWERVSANQVAVFTRQLSTLLGAGMPLVPSLSILMQQEKNPL
                     LKKSLAQIREQVNEGKSLTEAMAEFPRIFPSFYLNMVRAGEASGTINLVLERLADFSE
                     NQQALMNKIRSALAYPIIMFIMGSAVIFLLMTFVVPKITGIFTDMNQTLPLVTIVLIK
                     VSNFLKAFWWIILILLFGGLAAIRYMTTGTDSGRRAWDNIRLKIPVMGQINRKIAIAR
                     FCRTLATLLQSGVPLLTAMEIVRNIVNNVIIGDAIHQAARDLEEGKGLSGPLTKSGLF
                     PPLVTEMIAVGEQSGTLETMLNRIAAAYENESQASITVMTSLLEPIMILFMGLVVGFI
                     VVSILLPIFEMNQLVR"
     gene            complement(17207..18208)
                     /locus_tag="PLR13_03385"
     CDS             complement(17207..18208)
                     /locus_tag="PLR13_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQC18406.1"
                     /translation="MKKSLFYLLLISMLIFFVSACSDSDSDFDDTADAEYLTVQMRAD
                     AGNGEVSLDWQMLPRAETYNIYYMEDDEDDTNQPTSQKMKSEGTKIDGETNHIISAPY
                     VVTGLENGKTYWFALSAKNAEQAESDLSQAIYAIPRANPPLPAPKNVRANAGDKEATV
                     TWDAVTGAAGYIVYYYTSFANYQESPKITSTSYTFTGLTNKQTYLFFVAAVDSNDNNE
                     SGDSSASFVYTTTPSEDPPPSAPRNLRVINRGEGFVDLAWDAPLFHTGALTYNVYFFT
                     AGNITKSTAQKGIEALADTSTRAANLDKEKTHYFVVTAVDNNGESAESGEVSAIPDL"
     gene            complement(18362..19015)
                     /locus_tag="PLR13_03390"
     CDS             complement(18362..19015)
                     /locus_tag="PLR13_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC18407.1"
                     /translation="MEKDFDQMNYYEILDIGTNATSAEIRGAYNVALQMYQPDSLVSY
                     SFFSKGERDKILSLIDKAYFTLINEAQRAKYDNEMNLRETSVSADQHKAPPQPANIFE
                     FNRRDIASLTRKNNKSALKTKISQSQSIREILSRQKITGADLKAIREELGLAIETIHQ
                     ETKIRLDYLHDIEEDKTEKLPAPVFLKGFVKAYLRSLCIENADDISTAYMNTLPGKN"
     gene            complement(19037..19957)
                     /locus_tag="PLR13_03395"
     CDS             complement(19037..19957)
                     /locus_tag="PLR13_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18408.1"
                     /translation="MAHIWPIGGGKGGSGKSFVASSMGRLLAKSGKKTLLIDLDLGAA
                     NLHTMVDVPYPEKCFSDFVSKKIHDLEDTVLQTPFPNLFLISGAHDSLDIANLPYEKK
                     IKTLKAIVKLDYDYIILDLGAGTAFNTLDFFLASRNGIFITTPEPTSIENVYRLMRAI
                     YLRRIRHYFSAADFRTLEKKVREHLGEESFNKPENIVSIIKKEFPEKYALIRKDFNSF
                     RFKLILNQLRKQDNTVLGEQICKIIKKHLGINVEFAGNIAYDEHVHDAICQRVCFLDR
                     YPHTRAANDLRVLNRKMSNAFGEQLMLNYL"
     gene            20260..21579
                     /locus_tag="PLR13_03400"
     CDS             20260..21579
                     /locus_tag="PLR13_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQC18409.1"
                     /translation="MIHYEPEIRVALLQNYSEAHITLKGDFSSAHGRILEGCYTVTVD
                     DGWVLLADSSGKEIVRQKEILLQAGSRSFFTVSDVKIGIDFHWQRTQEQSFRGDLFLS
                     ASRDASFNLINNVHLENYLESVISSEMSAEAPLEFLRAQAITARSWLVSMLAKKQEAR
                     TSTPQRTGNEIVVWQDVNDHDGFDVCADDHCQRYQGITKIISENVHKAIESTRGHFLV
                     YGGKICDARYYKCCGGQTDVFDTAWEDKHYDYLSNISDDVKPHSPVCTEGDARRWLTG
                     RPSAYCNTSDEKLLKQILPAFDRETSDFFRWQVAYEREDLEDIIREKSGIDFGDLKNI
                     EPLERGPSGRISRLKIDGSKRTVMVGKELEIRRWLSPSHLLSSAFVVTVRRNTYGEVI
                     GFVFIGGGWGHGVGLCQIGAAVMASKGFQAEEILTHYFTGAQLKKLY"
     gene            21710..23026
                     /locus_tag="PLR13_03405"
     CDS             21710..23026
                     /locus_tag="PLR13_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQC18410.1"
                     /translation="MSETITETTRNAVRRNPWLWVPSLYLAESIPYTMAMTVSVVLYK
                     DMGLSNTQIAFYTSWLYLPWVIKPLWSPFVDIFRTKRFWILAMQLAIGASFAGVALTL
                     PTTDFVRYSLAVFWIMAFSSATHDIAADGFYMLGLDTPQQAAFVGVRVIFYRIATIAA
                     EGGLVFLAGMLQKYGYPAVGAWSLSFIVVAAVFLAFFIYHIFALPKPAEDISAPWDKS
                     KNFLAEYFRVLTLFFRRKDILIIITFFLLYRFAEAQLTKMVVPFLRDEISKGGLGLST
                     ADIGIIYGTVGVIALMLGGLLGGYVIYRNGLKYWLWPMVFIMHLPDLIFVYLSHYQPQ
                     NLWLVGSAVAVEKFGYGFGFTAYTMYMIMVAQGSYKTVFYAIGTGIMALGMMIPAMGS
                     GWIQDMLGYKNFFIWILITTIPGFIVAALVKIDPEYGKKINKEPRG"
     gene            23033..23749
                     /locus_tag="PLR13_03410"
     CDS             23033..23749
                     /locus_tag="PLR13_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQC18411.1"
                     /translation="MQNKQLNTAFILGAGLGTRLRPLTENTPKPLLEIGGHPMITYAM
                     AHLRAVGIKRFIINTHHCAEKYVEAFPEGNWQGTPITFCHEPVLLDTAGGIKNIEDLI
                     AEDERIIVYNGDIIANMPIELLIRKHFELKTSVTLALRSTGPLLNVSVDKDGFVCDMR
                     HILKNDGVKKCLFAGIYIVEKSFLKRLTTGKIESIVLPLVEMIKENPRSVGGVIIDDG
                     GWYDVGTVAEYEKLSKTDIR"
     gene            23854..24846
                     /locus_tag="PLR13_03415"
     CDS             23854..24846
                     /locus_tag="PLR13_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQC18412.1"
                     /translation="MQKEIQKFLTEHLGKTDFKLESIKKGGSDREFFRVSLPHGDSFI
                     FMRYGDEVVENANWAGINQFMASMDINVPRIILRDIPRHFILIEDLGDVDLWSLRFEP
                     WEERRNHYFQVLAQIHRLHSFDLKSTSPDVQLSESYGPRLYKWEHNYFLENLAWEVCK
                     IKLTSADAAKLNKELDALFESLQKIEPSLIHRDFQSQNIMIKNERPVLIDFQGMRQGC
                     LFYDLGSLLCDPYVTFEEKERDELLDFYYDIIRPAYSRDEFAHNFWAGSVQRLLQALG
                     AYGYLGLKKHKPAFLHHIPNGLENLLTAVDNAGGLKMLNDLATECETIIARRNI"
     gene            complement(24921..26714)
                     /locus_tag="PLR13_03420"
     CDS             complement(24921..26714)
                     /locus_tag="PLR13_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQC18413.1"
                     /translation="MVLHKIKQIVGWLRSFFLENITRWLVFGLLVGIVSGLGASAFYY
                     LLQAGRHLCFHLLAGYAVPAPAGEQVFDVPGILQFHPLIFFLLPALGGLISGLLVYHF
                     APEAEGHGTDAMIDAFHNKKGRIRTRVPVIKGLASIITLSSGGSAGREGPIAQIGAGI
                     GSAVARLLKLSNRERRILLLAGCGGGLSAIFRAPLGGALTAIEILYKEDMETEALVPT
                     VVSSITAYIIFTYFFGNTPIFFFPEYTFSNPRELIFYVLLGLICVPAGIFFIKIFYAV
                     RERIFLPLKIPRYVKPALGGLVVGCIGLVFPQVYGDGWGWIQLAILGKLSIGIMIAIA
                     LAKIVATSFTISSGGSGGVFGPTLFIGGMIGGTVGFSAHYFYPHIVTQPEAFVVVGMA
                     SFFAGVASAPIGTLLMCSEMTRGYGLIAPLMLVSIIAILFTRQHSIYTKQVQSRRESP
                     AHMAEFTVNLFAETQVKDFYKPEKVTPVREITTYGQLKRIFSASNVECFPVYNEDDVL
                     IGAINWDHARSIVFEEGLEDLVIAQDLMAPPQTVTPEDNFYDAFVKFMETNVEELLVV
                     SSDDPNRVLGVLRHDDLIAAYHAELNRRKYE"
     gene            complement(27162..28034)
                     /locus_tag="PLR13_03425"
     CDS             complement(27162..28034)
                     /locus_tag="PLR13_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQC18414.1"
                     /translation="MIIDCHYHLEERVFTVERLIGEMQKAGIDKVALMGSIIGPFTEP
                     PRILINLLQRLLENSFSRSIGKAFVSNFTDHGDVRILGKPYPLATDPDNEKVFNAVRK
                     YPDKFLGWIFVNPRGKNNQVEEYEKYKDEKGFIGVKAHPFWHHFAPVELAPVAERLAK
                     TGKPLLIHAGFGEEGNYEALLKKVPDLKLVLAHAGFPCYSDTWKKIKPLKNVYMDLSQ
                     TTYTSGKATRQAVDMFGAERLFFGTDGPFGFHGPDHQYDYGFIKRRIEGMFADVIIRE
                     KLLGGSFAKVVGLS"
     gene            complement(28071..30095)
                     /locus_tag="PLR13_03430"
     CDS             complement(28071..30095)
                     /locus_tag="PLR13_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC18415.1"
                     /translation="MNYRADTLCGLFHNQALRYGDRFVFLSGRFEADGRASDKFSSRT
                     WKQVREEAIALAKGLMALGIKKKDKVVIYSESRPRWIIADQAVQACRAIGVPLYPTVS
                     EEELDYMLEDSESCLAIVSTPEKARRVLKVTAGKAKMPVIMMSPWDDVGQKPEGVYTF
                     PEVFALGDPKVKLDVIEEGIRSVVPEDVASIIYTSGTTGKQKGVILTQSNWIHSMHQC
                     SGSEIMALTAQKDLHLKALVHLPLCHVYGRMSDYHTAGLKMGGELVFAESYQTIARDL
                     REVRPNIINSIPRLYEKTYEIVQSTLSRAKKPYQAIYNWAMSKGRIYVDCMATGKRMP
                     PHQLMLFGLANSLVFDRLKKEMCMDKLVMACSGGGKLSKDICIFYRALGIQLIEGYGL
                     TETTAINNLNAPEIMMEKPPTGFLKVLYDRIMALTLHLMVVRQSQGKSPYANPVMSLL
                     LSLCYNTLVYRLRVKPGFVGRVVPETEMRIAEDGEILIRGPQVFQCYWKREKDTADAF
                     TPDGFFMTGDIGVADEEGFLQITDRKKELFVTSGGKNVAPHPIEVALIERPYIDQACL
                     IGDGRKYLTAFIIPDFESLKRYAKDHGITCASQEDLANHPDIKALITKEVDHVNATLA
                     RYEQIKYFTILGRAFDVASGELTPTMKIKRRVVNEKYQQQIEEMYGKPNQ"
     gene            complement(30199..31692)
                     /locus_tag="PLR13_03435"
     CDS             complement(30199..31692)
                     /locus_tag="PLR13_03435"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HQC18416.1"
                     /translation="MRTKEQYIKGLRKMKRNLYFNGSKIDRDDEEQLPALNVMGFTFD
                     APHIPEFKDLCTAKSHLTGETINRFCHVHQNSDDLHRKQDMTRALCRTGRMCIQRCMG
                     VDAINGVNAASYEADKQNNGSTEYHKNFLKWLENFQRNDLVGCCAQTDMKGERLKRPA
                     EQTDPDMYLRVVEKKKDGIVVRGCKLHISEASIADEIIVVPTRILGPDEQQFAVSFAV
                     PADYDGVKQVVHIHKIRKRTHFKTGNPDIGYTDSYVIFDDVFVPWERVFLCGEYVHGG
                     ALAMLFALFHRHSYSGCKPAIGDMLLGTASLAAKMNGISKAHHVREILSRIIMVTELG
                     YAAGFTASAKSKPEIYVPGIGQMPYGPGSYIPHSIYANVGRCLTGEAVFDEQEMLCNI
                     AGGLPSTFPYEADLANPELKPLLEKYLKRNPKIPIADQIKFWLLFAEATCSSSTGFMC
                     YGSYHGGGSPIMEQIAITLQYDTKLREHLVNAAAGIEKLDYGRVTKI"
     gene            31962..32666
                     /locus_tag="PLR13_03440"
     CDS             31962..32666
                     /locus_tag="PLR13_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC18417.1"
                     /translation="MARHDIKEARKDNLIDAALRVFAEKGYSDTTMTDIARKAGVSTP
                     VLYEYFKTKEDLLFAIPEKFMEEPIRIMEYVAPYLRGAEAKIRGIVQGYLTVYDENPL
                     YASLVMLELKTNRKFLKAKAYGMVRKVARGLLEVIEEGIKDGTFKKDTDAFLVRSMLL
                     GTIEHLCIRKLLLGEPKNLPQYTDAIVNIILNSIKKESRQFSVNLNINDLLSPNIKTE
                     NIPANKGTSDPKRKKR"
     gene            complement(32719..36402)
                     /locus_tag="PLR13_03445"
     CDS             complement(32719..36402)
                     /locus_tag="PLR13_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQC18418.1"
                     /translation="MISENIRRLVKTVVHFAGRCHIYVGRRPDHVARPSLIFFPVLDG
                     YLCCGFAGLMTCGLKSQPPQTRADLTLVALWKKLKSANVKNPSRLPDVTETVNAMADT
                     VAQLKKEEAQEFLFFHPDYSSDLSVFAADLNRFIAEKENRLDEQTAFLTSAELESVNS
                     LIVMLKDIGWALEKDILANLPKILALTGAGNRADIRPAAFRKYRKLNLALNALDRLEV
                     RGRDSAGIDLTFAPESENDLKAALQRITEQGWLADYEKRTQPGDLLNLSVSVTKNTRG
                     TNAGIVVTFTYKTFSTVGKLGRNVAELRNSIRNDRILRCFADLETSCENVLMHTRWAS
                     VGSITEENCHPVNNHSMIRFACPSTGYPESDVQIHVVLNGDVDNYTPLRQFLETQGEM
                     IARPITTDTKIIPLQIEKFLHSGHTLTEAFRRAVNDLAGSHAIAMTSHLEPGKMFLAL
                     RGSGQSLYIGVSDDQYMFSSELYGLVEVMPRFLKMDGEAGGQICILDQNGPAGVRGIT
                     SYSYDGKILALTENMLGTAEITTRDIDRGDYPHFFLKEISESSLSVKRTLRGKYRMTA
                     TEAALQQVRFNLGEDIVPADIIAGLKKGRIKNIVIIGHGTAAVAGQAVADAFSLYLGS
                     SPLHITARVASELSGFGLKDDLSDTLVIPITQSGTTTDTNRAVAMAKERGAHVISIVN
                     RRQSDITAKSHGVFYTSDGRDIEMSVASSKAFYAQIVAGQVLALFFAQSLRTCTDEDI
                     IACLRNLESAPLLMNQIASDCASVASTVKDAIGKKYWAVTGSGPNKAAADEIRIKLSE
                     LCYKTISTDIVENKKHIDLSAEPLILVCTAGSPESVTADIVKDVAIFKAHKANVIVIA
                     DEGEKRFQGVADAVIAVPVAFPPLPVILNAMAGHLWGYYAACAIDEEAQIFRAFRARL
                     HDILMLKAREQLSVFDLTADVSVKGIINTFYQSFNTRRQQGAFSSLEGKTLSDLMLLL
                     KYAAGKLPLKDMALDFVNDQGIFSPFELLDTTLGTVIDELSRPIDAIRHQAKTVTVGT
                     SRKERPPEGIVFDLLKTLTFDIATLTYRDIMMIRRIQPVIASIPGYTIYDISALDQQG
                     NPTDDSLIAVRVKGGLAAGMKSRADKPAVLMGVKRTIVSTGHAYLGRGKTDNAPILII
                     PLRTQEAGIGNLLLLHVRFDEKLTLQEKIDALGYRYNDIRNLIDEYNLKWDDRFIDRF
                     SLEALFSEPIEIIAGRIKSQLNP"
     gene            complement(36410..39019)
                     /locus_tag="PLR13_03450"
     CDS             complement(36410..39019)
                     /locus_tag="PLR13_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQC18419.1"
                     /translation="MTPYARVSTGTSSLDDIINGLQMGDNVVLQVDDIEDYKKFVEPF
                     VKTSLASRHRLVYMRFAAHPPLLEANKKIKVYKLNANNGFESFSTQVHNIIKEEGRDV
                     FYVFDCLSDLLMAWATDLMIGNFFVITCPYLFELNTVAYFAILRSSHSFKTVARIRET
                     TQVLLDIHNSNGQLCVHPLKAWKRYTPTMFLPHIMENDKFVPILNSADAASFLSYLTN
                     KNTTGGVRNLDYWDRLFLKASNLLEDPDAVLDRQEMVDSLSRVMIGREKRMLSLVKEY
                     FSLEDLLEIKKRLIGTGFIGGKSVGMLMARNILRKDPAFDWDSELELHDSFYIGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFEVARELKSRMLHGIFPDEIKEQFQLMLEYFGQSPI
                     IVRSSSLLEDAFGNAFAGKYESYFCPNQGTPETRYKNFEDAVRKIFASTMNEDALTYR
                     VQRGMAHQDEQMALLVQRVSGSHRNRYFFPDIAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPLIKPHAKQEDIRRFSQNEVDVLNLKDNHFQTLPT
                     SDLLDYAIEPHIGMIAVRDAAVDEYYQSLGREAKETWILTFDELLENTPFARTMSDML
                     KKLAQVYRYPVDIEFTANFNAEGKPKINLLQCRPFQTKGHYSRVELPEKIEKSRILLR
                     QEANFMGGSVYQAISRIIYIDPRGYARLTVSEKHEVARLTGKLNKQIGKREKMPTILF
                     GPGRWGTTTPAMGVPVTFSEINNIMAIGEIAYQDGALIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKEGVFFNTSWMAKQPNILADLMPEDGRFAKVVKVFDMRNQDLRLLSDIVTQK
                     MICFFGDASAIGK"
     gene            39541..40773
                     /locus_tag="PLR13_03455"
     CDS             39541..40773
                     /locus_tag="PLR13_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQC18420.1"
                     /translation="MDSGNTAWVLTASALVLLMTIPGLAFFYGGLVRRKNVLSIMMQC
                     FVIVCVISLQWMLFGYSLAFGPDFHGIIGKLDWMGLAGVGATPNPDYSGGIPHYAFMI
                     FQAMFAIITPALIIGAYAERVKFSAFLVFTLLWATFVYDPLAHWVWGVGGWMRTLGAL
                     DFAGGIVVHVSSGISALVLALMLGKRIGYNHKPIRPHNLPFTVLGAALLWVGWFGFNA
                     GSALAADGLAANAFVTTHIATAAAGITWALIEWWHNGTPTILGAATGAVAGLVAVTPA
                     CGFVNPMNAMFIGMIVAVVCYIAVAVIKGKLGYDDSLDAFGVHGVGGTVGTIATGLFA
                     EKVVNAAGADGLLFGNAHQLYVQSLSLIVTVAFAAIMTFIIFKLVDALIGMRVEENNE
                     VVGLDLTQQSEAAYTVIE"
     gene            40786..41127
                     /locus_tag="PLR13_03460"
     CDS             40786..41127
                     /locus_tag="PLR13_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQC18421.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVDVNLMTVTNVLGQGRQKGVTE
                     IYRGAKEAGGLLNKVRLDIAVNEDFVEPTVKAITKGAHTGSVGDGKIFIVELAECIRI
                     SSGERGGLAIG"
     gene            41138..41341
                     /locus_tag="PLR13_03465"
     CDS             41138..41341
                     /locus_tag="PLR13_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC18422.1"
                     /translation="MSIRIHKAKTNALARCVSECEKAVIMHVLLKTKGDQSEAASLLG
                     IKESFLADKIHQYEIDCARFELK"
     gene            41363..42283
                     /locus_tag="PLR13_03470"
     CDS             41363..42283
                     /locus_tag="PLR13_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18423.1"
                     /translation="MKDNLKTGIIMVLLTLILALLPVMSWADEEIVPADPKQGDNAST
                     EVKPVEQDQPEDKVTGEVAASVLSAYIWRGQELTRHSVVIQPSVTAAYKGFAANVWGN
                     LDTKPYSALDVDYSSNFTETDVTLSYLHKFGILQAGIGYIYYGLAGAVQGGADLPDSQ
                     EVFVMLGLDTILAPTLTVYKEFDYYNQWYATLGISHTFALHEKVGLKFAAQASYLRSD
                     DEATYPEFDSASLATTDKFNNLHDGVVSVSLPIAVTGHLTISPVLTYVFPLSDDAKYE
                     MKARGLKGDIDPSHKEGDFLYGGIVVSYAF"
     gene            42332..42405
                     /locus_tag="PLR13_03475"
     tRNA            42332..42405
                     /locus_tag="PLR13_03475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42364..42366,aa:Gln,seq:ctg)
     gene            42466..43173
                     /locus_tag="PLR13_03480"
     CDS             42466..43173
                     /locus_tag="PLR13_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQC18424.1"
                     /translation="MRAHYFQHVPFEGLGSIESWLKKSGFKITSTRFYESAKLPDLKT
                     IDLLVVMGGPMSVNDEDRFPWLVAEKQFIWDAIAKSKPVLGICLGAQLIASALGAGVY
                     PNKVKEIGWFPIHGIESNGQSVFAFPESISVFHWHGETFDLPSGATLLAKSDACEHQA
                     FQVGKSVIGLQFHLETMPAAAQALVTNCRDELVPAPFIQTEEEILSTTPDQYTAINDL
                     MADILSFLLTQKMTAGL"
     gene            43413..44951
                     /locus_tag="PLR13_03485"
     CDS             43413..44951
                     /locus_tag="PLR13_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQC18425.1"
                     /translation="MEERESQLSIHTYEELAALHAIAQILAQPGDLHHQLALILKEMS
                     SRLGMQRGMISLVDRDNKESWLDVAHDVNIDGLEITYKHGEGITGKVAETGRPMAVAN
                     LGQETHFLDRTGARRHLNRAELSFLCVPIIYDFMVEGVLSADKVAVKVENLDKELALM
                     ASVAELMAKAVHIRALEEENKRLRKIIGSTRAPLMEIIGHSKAMQEVFGLVAQVADSN
                     TTVLITGETGTGKELIARAIHKNSVRKNGPMVQVNCAAIPDTLIESELFGHEKGAFTG
                     ALTQRRGRFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRVVNVNVRII
                     AATNRNLEQDILSDRFRADLFYRLNVFPIYLPPLRERGSDVIMLADHFLLKYSKEMGK
                     QVKRISPAAIEVFLSHKWPGNVRELENCIERGVLMTKTDVLDCEHLPPSLQLKDGMHD
                     RKGRGKLSSVVEAQERLLIIDALKETSGNQTKSAKLLGTTKRIIQYKISKMGIDPKNY
                     RKKS"
     gene            45216..46328
                     /locus_tag="PLR13_03490"
     CDS             45216..46328
                     /locus_tag="PLR13_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HQC18426.1"
                     /translation="MTSNDPVSPLAAIRAIDVMREGHDGSGVGLFLTDLGSDFQEFKN
                     EPILSGIFSNEGLKTLDRFMIDHDFMVKYKLSIRPAKNPPAGTPKRDNYVIRIYEYPE
                     EWDGLCRDEIEFRLMMVQLELRRMGEQDESMLVFSFWPDVIIIKEVGDPLAVAEYLGL
                     DRKELMARVILSQGRQNTNYAINIYACHPFFIQGMATVTNGENTAFVPIREFLMSRKF
                     PGYTGYHSDSETFTHILHYMYHQLGMGMEMYKHIITPLKDGELNRHPDGVWLRKLKQS
                     CRPLIIDGPNCVIGCLPDKSMFMVQDSKKLRPGVVGGRPGVFAFSSEMCGLDAALPDR
                     YVNLDFQPMKYETVIVRSERQEIEKWNQLEPLTHPH"
     gene            46292..47926
                     /locus_tag="PLR13_03495"
     CDS             46292..47926
                     /locus_tag="PLR13_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQC18427.1"
                     /translation="MESAGTVNPSTLSVKDLPWQIMWNKEKCTLCGRCTAVCPVRAIE
                     PGVHRKRALQVPVGLEEKPAQLLSVYFGIDQRTDAAHACVGCGMCTLVCPNGAIMPVR
                     AEETDKLRFHVNQGGEPRRRGGRRNVPDSVLDKIKFIRISMLTDPALDAGRHEFELRT
                     LLGRVLPPEELLKVHRENGWVPPVREIYPLVIGGMSFGALSPNMWEGLQMGVAYLNEE
                     LGMPVRICTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEIIHGLPQMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNKLIAAIRGVPQGVSLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTLAVLNNLTRNPYAAALAIDGEDGGTGAAYNVSMNHMGHP
                     IASNLRDAYLNLVKIGKQNEVPLFAGGGIGKNGNLAANAAALIMLGASGVQTGKYIMQ
                     AAAGCLGSETDRCNICNIGACPKGITSQDSRLYRRLDPEKVAQRLVDVFLSFDTELKK
                     IVAPLGRSTSLPIGMSDALGIADAGAAERLKIKYVL"
     gene            47948..50284
                     /locus_tag="PLR13_03500"
     CDS             47948..50284
                     /locus_tag="PLR13_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC18428.1"
                     /translation="MTKKESLKVTGQENGIRVESRILEERIQQAVAEGYRHIEITAYG
                     QHGIGGRLWRAGETPIKVDVTGTSGQRLGSMGFANTLIEVFGPVSDDVGWLNAGAKII
                     VHGNATNGVANAMAQGKVFIDGDIGARGMTMTKHNPRFDPPELWVFGKAGDSFAEFMA
                     GGIAVICGVGAENEDDVLGYRPCVGMVGGKIFFRGRQSSYSTQDARLITDLPDEEWRW
                     LTKNLKAFLQDVGKQYLCEELTARRSDWKLLSALKPHEKKGRALSPMTVYRKEAWEKE
                     LGTGGLIGDLTGADQSPIGLITTGDLRRFVPVWANEKYLPPCQASCPTGIPVQKRWEL
                     IRKGKMKDAVDLALRYTPFPATVCGYLCPNLCMQNCSRKIVSLPSVDVTVLGKASLEA
                     KEPKPLPATGKKIAIIGGGAAGLSVAWQLWMNGHDAVIFESRKHLGGKITQTIPQSRI
                     PDNVVEHELKRLAKNLRHIHLNASLTKEKFRKLKNEFDFIVIAAGAAKPRKLNVPGME
                     KALTALEFLKQSKGNGINVGKKVVIVGAGNVGCDAATEAFRLGAKTVTLIDVQQPASF
                     GTERKHAEAAGAIFLWPRFTKAITARGVELTDGEILPADTVIVAVGDVPDISFLPEEI
                     AVKNGFIAVDEQFRTSDDQIFAVGDVTKPGLLTEAIGAGRIAAGTIDALLRGAGETYD
                     KLPPIDVQRVKTEYFDSRIKAFSDPVSCAVNCASCGACRDCGMCETICPQNAISRKAL
                     NGENYEYVVDAELCMGCGFCAGVCPTGVWEIVDNKPLE"
     gene            50315..>50554
                     /locus_tag="PLR13_03505"
     CDS             50315..>50554
                     /locus_tag="PLR13_03505"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQC18429.1"
                     /translation="MKKTKKTTAAEREYVIEKEKFVPVSKYFGEDTFNQKTMKERLPK
                     ETFKKLMDAINEDKRLDLKTADVVAEAMKKWALEKG"
BASE COUNT        12267 a        12179 c        12728 g        13280 t
ORIGIN      
        1 ataaaaaatc cggcaattcc cagaacaacc agaaaaataa gaagaccaat cgcgattttt
       61 tttaggatac gcataatgcc tccttaatat tagaaaaaca ttaattgtca ttccagcaga
      121 ggccggaatc catttgttgt ttgttatttt gagccaaaca aagacttgat tccttttctc
      181 ctcatctctt acttcttcct acccataagt tatttttatt aaaatatttc gattcagaat
      241 aaccagctgc attccataaa gtgtcaaacg caggtctcat gactctttga taatcttgtt
      301 ctgttccgta atcctcaatt attatttctg gaagcagcaa ctcagaaaat ttaaatggaa
      361 cgggattgtc aacggtccaa atatcacccc ctactcctaa cgacgcacct gaaataccct
      421 tgttcaatcg agtcttttcg actcgattga ttttagatct gattaaatgg tcgagtcaaa
      481 aaaactccac ctaacaaaga ctttcttttc agtagctttg tattcaaagt aaaacgaggc
      541 ggcagcaagc agacaccgca ggcgtatatg acaatacgtc gaggatgact gtgcagctgc
      601 caacaaagtt atacgaagaa tacgaagcta ctgcttgaat tctttttcta ttctctccat
      661 cgcttcgcgt agccgttcat ccggcaccgt cagcgagatg cgcacaaaac cctcgccgta
      721 ctggccgtag gccgtaccgg cagcaacgac cacagcggtt ttatcgaaca ggcgattggt
      781 gaattccagc gaggtcatcc ctttgggaac aggcacccac agataaaacg ttcccctggg
      841 cggattgaat tcaatgccga ttttttttaa tgtcttaaga acgagttcgc ggcgctttcc
      901 atacaattca atcatcttgt caacaaaagg cgcttcgttc tgcagggctt tgatgccggc
      961 gaattgaatc ggattaaaga ttccagagtc cgtgttttcc ttaaccttgc tgatggcggc
     1021 gattaattcg ggattgccgc aggccatgcc cagccgccat ccgcacatat tgaagggctt
     1081 ggataaggag ttcaattcga tgccgacgtc tttcgcgcct ttggccatca gaaaactgat
     1141 gcgtttttgt ccgctaaaaa ctatttcgct gtagggattg tcgtaacaaa cagcgatgtc
     1201 gtaggttctg gcaaattcaa caagcttgtc taaaaattcc agggtcgcgc aggcgccggt
     1261 cggattgttg ggataattca aaaacatggc ggtggctttt ttagcgatgt ccgtcggaat
     1321 gtcttcgaat accggcagat aattgttctg cggcaggatg ggtacattat acggaacgcc
     1381 gccgcccagc aggatgctgg aacgataagc gggataaccg gggtcggtca tcaaaacgat
     1441 gtcatcggga ttgacgcgcg cgatgataaa atgatgacag ccttccttcg aaccgatcaa
     1501 tcccagaatt tcgttttcgg gattcagcgt aacgccgaag cgtgtttcgt accatttggc
     1561 aatctcgcgg cggtatgcca gcataccctt ttcttcatcg gtcggataac ggtgattcag
     1621 aggatcgcgc gacgtggcgc aaagctcatc aataatagaa tccggcgtgg cttccaccgg
     1681 atcgccgatg gccagtgaaa taacatcaat gccttcggcc ttagccttgt ttattttttt
     1741 cctcaattcc atgaacaggt aaggcggtat tttcgctagt ttgtccgcag ttttcaaaat
     1801 cgatatcctc caaaattaat tatgattaga atctaattgt cacaccggtc gtagatgacc
     1861 ctatagtgac cttcaggtta tttaggttac gaaggccgat gtccagaaat tgatatttaa
     1921 ttaaataata ctggattccg gtccgcaccg gaatgacaga tatattttat taagtttatt
     1981 gataggcttc ttccagaagc attccctgat agaccatttt gacacggcct tcaaggtaaa
     2041 tttcacgaca caggccgttt tgattttcaa aataaacgcg caggctttca ctgcttcgta
     2101 ccagcacgtc cacgggcgat tcaacaagat ttctttgcgc tgccgccaga actgccgcca
     2161 ccacacccgt tccgcaagcc agcgtttcat cttcgacgcc gcgttcgtag gtccggaccc
     2221 ttattttatt tctgttaatg agtgagacaa aatcggcatt ggttccccgg ggggcaaagt
     2281 gttcatggaa acggatgttg cgtccctgac gatatacttc gcaggtatcg agatcatcga
     2341 caaaaactac cgcgtgcgga accccggtat caatactgtc caagatgaat ttcagcccgt
     2401 ccgtttcgat ttcgatattg gattcaaccg gcgagggatc ggtaagtttt gccttgacca
     2461 cgtcgtcatt tacaaccgcg ctgatgatac cggcaatggt ttcccaagac attgtttgtc
     2521 ctgcgattcc tttcatataa gcgaaacgcg ccacgcatcg ggcggcattg ccgcacattt
     2581 ccgccatgga accgtcagca ttgaaaaaac gccatttgaa atcggcgatg tcggacggct
     2641 caatcaccac cagtccgtcg gcgcccacgg aaattttgcg ctgacagact ttgcgggcga
     2701 actcggataa atcaccgaca tccagggaca aatccatgtt gtcaatgatg atgaaatcat
     2761 tgccgctgcc gctcatttta tagaattcga tcatgtttta ttccgtttcc agtagcgaga
     2821 ctcactatcc cactccgctt cgcttcgggg ataattcggc taactcttca ctatagttca
     2881 gagagcctca ttaaacttcg atgacgatgt catcggtacg actgagagtg cgggtaactc
     2941 tcagcacttc ttcgattgtc gtgatgcctg cgagaacctt gtcgatacca tcctgctgta
     3001 atgtaatcat gccccgcttg atggccagtt cattgatctg attggcgtcg gatgttttca
     3061 gtacgagcct tttaatctca tcatcaacgg tcagaatttc gaaaatggct gaacgtcccc
     3121 ggaaacccgt ctgcatgcag agattgcagc ctttcttgcg gtaaaaagtg ttgtttttca
     3181 ggacggatcg gctcaaaccg agattggcca gcgattcttc atccggctca taaacctctt
     3241 tacaatgggg gcagagaacg cgaacgagtc gctgggcgat gatcgcgaca atagacgatg
     3301 tgaccagaaa aggttcaatg cccatatcga tcagacgggt aacggcactg gcggcatcgt
     3361 tggtatgcag cgtggagaag accagatgtc cggtcagcga ggactgaatg gcgatttcag
     3421 cggtttcccg gtcacggatt tccccgatga gaatgacatc aggatcctga cggacgatag
     3481 aacgcaggcc ggcggcaaac gtcagatcga ttttgggatt gacctggatt tgtcccacgc
     3541 cgtccatttg atattcgata gggtcctcga tagtgatgat attgatctcc ggacggttaa
     3601 tggtggaaag cgctgcgtac aacgtcgttg ttttaccgct gccggtgggg ccggtcacca
     3661 gaatgattcc gtaaggcgat ttaatcagct tattgaggag tttgatccgt tcatcatgca
     3721 tgcccagatc ggataacatt aaaatgttgg ccgttttgtc cagcaatcgc agaacgactc
     3781 tttcgccgaa ggcggtgggg atgaccgaca cgcggatatc caccaggcga tcggctattt
     3841 ttatttcgat gcgtccgtcc tgggggagac gtttttccgc gatattgagt ttggccatga
     3901 ttttgatacg tgatatcagc ggcgactgga tgcgcctcgg caggctgagt gtatcatata
     3961 aaatgccgtc aatgcggtag cggattttca ggtttgccga atagggctcg acatgaatat
     4021 cgctggcgcg gtccttgatc gcgccggaaa gaatgaggtt gaccagtttg atgatcgggg
     4081 catcgctggc ttcatccagc agatcggcgg tttcatcgat tttggaaatg agatcgtcgg
     4141 tggaggactg gtccatttct tcaaaataat ccttggccga tgagcggctc atgtcatacg
     4201 ccatattaat ggccgacata attgcgtcct gcaaagacag aacaaccgac agatttggat
     4261 tcttcaggag ctgtctcaag tcatcgacag gctggaaatt cgaaggatcg tttaatgcga
     4321 tcaccgggtt ttcggcggtg atcaatggaa tgattttatg tttttttaaa tattgaatgg
     4381 ggatgcgttg ggtaaagtcg atatcaatgc tttccatggg cagttgctgc cagaatgaca
     4441 gcccgaaatg ttcggaaagg atttttaaaa gatggatttc aggaatcact ttctttttga
     4501 tcagttgatc gaaaaatcct ccgccgttgt ttgccggtgc ggttcgtatt tcactgaggg
     4561 tgtccggtgc cactcccgcg gcgatgagct tttcatccag agacgaaaaa acggatttgc
     4621 cggacgatgc atcaccatga gcagacgccg ccgattcttt cagagctgta tggattttgg
     4681 acatatcgaa tcggaccctc aggcttacgg attggaaatg gtttcgtttt caacgggagg
     4741 acgcacctgg ggagccttcg catcggcttt ttcattcagt ttgatgacgc cctcgaccac
     4801 ttctcccatg gtctcccttt tttcctgata aatttttgcg gcatcggcct gcgtacggac
     4861 gatgtgcggt gtcagaaata cgtaaaggtt ggtcttttcc cgtgatgatg ttttcgtttt
     4921 gaaaagccag cccagaagag gaatgtcgcc cagcaggggg aatttgtacg tgctgtcgtc
     4981 cgtactgtcg ccgaccagac cgccgatgac gaccgtttca ttgtctttga cgatcaccgt
     5041 tgtctttgcc gttctcttca gcgtggtcgg tcttgcttca tcggcgcccg actgcacctt
     5101 ggtcacctgc tgatcgatct tcagccggat aaagttttcc tgattgatat tcggcgtgat
     5161 ttttaaaatg acgccgacat ccctgtattc atagctgctg tagttcgtat aatttgtgcc
     5221 ggtgcccaca tttgtttgat cttgtcttgt cacataggga acgttcgacc ccacattgat
     5281 ttccgcctct tcattgtcca gtgtcatgat ctgcggggtg gataagatgg aaacttccga
     5341 atcggtttta taggcctgaa cgaccgcacc gatgctgggg aaggtgacgc cgccgatctt
     5401 aatgcctgcc ccgaccagtc ccatggaaaa accgctggga aagacgccca ttccggtcgt
     5461 tgtcagcaaa ccgtccatga tattgtagga accgggaccg gaagggccgg cgcctcccga
     5521 accggcaaaa gccgccgtgg gatttccgtc aacggagcca tgttcggtga tgccccgcca
     5581 ttcgacgccg agtttgaaat ccttgttggc gttgacttcc ataattaaag cttcaatata
     5641 gaccatcgga cgcggaatat cgagctgctt gatgatgttt tcaataatct tgtagtcttc
     5701 ccgttccgcc atcacgacca gcgtgttggt cgctttatcg ggaacgattt gaacattttt
     5761 ggaaacgaca gtcgtcggtg ctgttttcgc tccggttgcc gttgctgtgg cgccggctgc
     5821 tgccccgcct tccttgacaa tgctggtgag cacttttgcc agatcttcgg cgacactgtt
     5881 ttgtaaacga taaacctgaa tattggattc tccgcgcggc acatccttgt ccataaatgc
     5941 gatcagcttg cgcacatttt ctgtttcggc gccgctggcc agcagaatga ttgaattggt
     6001 gcgcgaatcc gctacgactt ttaagattgt cgcggccttt ccgcggacgg gaccctgtgt
     6061 gaagatggta ttcaacgatt tgaccacttc cgaagcggaa gcattctgca gcgggatata
     6121 ggatatctga tcgccggcgc cttcgacatc cagcgcggtt gcgatttcct gaatccgttt
     6181 gatgttggaa aggtaatcgg taatgatcag catgcctgcc ggcggatagg aaagaacaga
     6241 actggatttc ggaataagcg gatccaggat tcttttcact tcatcgggat tggcccgctc
     6301 caggggaatg atctgagtaa ccatgcgatc ctccggcaac aggggatcct gttttgtcct
     6361 ggtttccaga cctttctccc gtgcctgcac agagggaatg attttgatca tattgcccga
     6421 gggcaccacg gtcaaaccgt taatttccag aacggacaga aaaactttat agacatcttc
     6481 cacgggaatc tttttggggg aaacgatggt gaccttaccc ttgactttgt cgtcaatgat
     6541 gaaattttta ccggtcaatt cactgacgaa ttttaccagt acgctgacat ccacattgtc
     6601 gaaatccagc gtgacatatt tctgcgacgg attgacttta atgggcttgt aagaccgttt
     6661 ttcatctttg ttgtccgtag acatgaccgg tgcgtccatc tcggcattct gacgggaagg
     6721 ttcattcatc agctcgggtt ccggcgccgg gtccacctct tcttctatcg ccggttccgg
     6781 agcggcatcc ggcatagccg tagtctcgat ctcttcaccg ccgccggcga cacgcgcggc
     6841 cagcgctgaa ccggcaaaac cgcttataat caccaacagg atcatgaccc atattaaaaa
     6901 accggctttc aaaataagaa catgtgattt tttcatcttg acgttaattt ttctccaata
     6961 gtttcttaaa ttccggcaaa tcggccagaa cctcaagatc tttgtctttt tctgcccact
     7021 gtctgacctg agggttaaag ccgatagcat ttttcaaata ggaaagactt cggctcaggt
     7081 cgttgttttg cgcgtagata caggccagat tgtaatgcgc atcggggtaa tcatgtttta
     7141 ttttcagcgc gtcgttgagt cgaataattg cccatttgta agttttcttc ttcatataaa
     7201 cgacacccaa attattcagc gcttctatat tcttcgggtc aatctcaata actttcttat
     7261 ataaatcctt tgcctgctcc aggttgcctt cctgatgttt ccgcagcgct tcggcgtaaa
     7321 gttttactga atcggctgtc cgtattttca atgcagcgct ttcttcggtt ttttgagtct
     7381 ttttttctgt cgtttttaat accggctcga ctttattttc aacctttatg tcttgcacag
     7441 ccggttgaac cggtgcctca atcaccggcg tttcaatcac cggtgccgtc ggaatcgtca
     7501 gcggcgcttt ggccgtggct ttttttgttt cagggccgga catcagaaca aaaagtcctg
     7561 ccaccgccac acaaaaaaac aatgccaaaa accccagcag ggccagccat cgcctccgcc
     7621 cggtattttt tttaccggaa ggcgacaaaa gcggttcata tgccgcatag ggtgattttt
     7681 tgtcctgctg tgctttgcgt aatgcgtcgt ttatgtagct cataatatcc ctttggtcaa
     7741 gactttccag cttcggtcag acggtcagac gcgccagtaa ttttctgccc agactcatgg
     7801 ttttttgaaa ataagttgtg ccgatgtcgt gaactgccag cctgacgatt ttgcgatcta
     7861 ttttcgttat gcttttggta taggcgatca ggagcgcccg gtcgcaaatc gtgttaatcc
     7921 gcctcgggat gccttccgaa aaattgaaaa tgagcctgta ggcagcgccg gtgaaacgaa
     7981 tgtcccccgg gccgtgagca actttcagac gatgatgaat gtattcgcgc acttccggag
     8041 gtgatagcgg tttcaaagca taccggaccg tgattctttc attcaactgc cgcagagcgg
     8101 gcagcatcaa atggttattg atttccggtt gtccgatcag aataatctga atcagttttt
     8161 ccgtttcggt ttccagattg gaaagcatgc ggatctgttc cagcaccgca tgcgatagat
     8221 tttgcgcctc atcgataagc agaacggctg ttttgccggc ggagaaattt tccagcagaa
     8281 aactgttcag gcggtcgata taattttttt tgcttcggga ttcgcgttcg atgtcgacgc
     8341 cgtattcttc gagaatggtt tccagtaaat cgatatcgga aacggcggta ttgaaaatga
     8401 gcgctgtttc caccgaatca tccagtgagg ccagcagact gcggcagacc gtggttttgc
     8461 ccatgccgat atcgcccgaa agcagaacga accccttttt ttctttgatt ccgtaaatca
     8521 agcagtttaa ggcgtcgcgg tgctgttcgc ttaaaaaaag gtagcgcggt tccggcgaca
     8581 agctgaaggg attttctttc aacccaaaat acgcggcata catggatatt tttctcccga
     8641 ggcaatcatt ccaacgtata attaatcgtt tccgtccgtc ctcttcgctt gacggtgagt
     8701 tccaaacggc ttccggactg catcgcattt aaaacttgca tgacatcgtt ggcgttgtgc
     8761 aacggattat tgttgattcc gacgatgatg tcgccgttct gaagcccggc tttttcgtaa
     8821 aggctactcg gcttgatatt ggaaatgatg tatccctgac gcacgcccct gctcatgaag
     8881 ggacgaacaa ccgcttgatt cataatggat tgcaggtatt ttgcgtttcc atcaattccc
     8941 agtctggaaa gagcagggtt tcttgaaggc gagggattcc tcctggagcc gggggcaggg
     9001 gaaagcaaat tatcttccac cgtctctttg atttttactg ttatttcttc ctcgccgttt
     9061 ttcagaacgg cggtgtttct tgttatttta gccagcttgc gggcgccgat cttgtccccc
     9121 aggcggtata acttctgttt tttgcttccc cgttcctcaa cgataataaa accggaagag
     9181 gaactaatgg ctatggttcc tttcagatcg aaatccatgc ttttctggtc ggcatcgaaa
     9241 ggcccgtccg cagattcgct tccgcctgct gttctcaacg tcgtcaaaaa cagattgcgt
     9301 tcggtgatga tatcgtaatc ctgaagctgc tggtgctgcc ggttgttcct gacgaatgat
     9361 tcatgatggt ttttgataac ggcagtgtgt ctggtcagcg ggaaacggac cactttataa
     9421 aacaggtcgg tcgcctcgta agacagaatg gtgatgacca gtacaacggc cacagagatc
     9481 aagacaccgg tatccaaaga tgaaacggag ggcattcttt tgaaagaccc gatacgttct
     9541 ttgatctgcg ctgtaatttc aggaaacatc atcttaccta catatagcga atagaaggat
     9601 tggcaatggt accttcaatg gtgatattca tttttacctt tctacccgat atgattattt
     9661 cgcccgttag atttaccggg ctgtttttga attcgtcaac aagcatgatg tcgccgttta
     9721 aagaacagtc catttgcggg ctgaagatct ggaacttttc gaatttcagg acgcggtttt
     9781 ccagcctggc ctgaatctcg ccacggtcaa aatcgattcg ttgcagtccc aggaagggtt
     9841 ccgccagcgg ataagatccc ttgttgagga aaagcgaaat ggttgcggaa gagttttcac
     9901 gtccccctga tcccgccgcc tgcgtccagt taccgctcgc cttgcctgtt atctgtttac
     9961 cggttaacgc tttgatgagc gtgtaccggt ccagcgcgat gttcttgaat ttcagcatcc
    10021 cttcctctgg cggatgaaaa ccggccgccg tggtcttgaa atttcccgaa aaggttccgc
    10081 cgtatgcttt tccgctcagg cggatatttt ttttatcccg gaaaaaattc aacgggtgaa
    10141 actgtacgtc gagggaatct ccctgaaaat aaatatttgg ggaggaccct gaacccagac
    10201 ggacattttt cattttcaga cccaagggca gggacggttt taatgagccg gttttcaaaa
    10261 tcaaaccgga ggaggcaatg gattcttcca gcctgctttg ggcgatatcc gagggaaaga
    10321 gcagatacag aaagaccagc gtgatgaata tcccgtaaat cgtaaaccag aatatcttgt
    10381 ttttcagtat tttcatggtt gcccgctttc cggaatttga ttcgacggct gaatggaaga
    10441 tatctgcaat tgcacactta aatattccgg agcgtccttc atttttacaa tatggatctt
    10501 tttgactctc actaaatccg ccggcgattc cgcgtaataa aggaaatcaa cgagctgttt
    10561 gatggtgacc ttctccagct tcatatcgat caaagactcc tcaaaggagg cggattgaat
    10621 gcctctggag gagttcatgt ttttgattct gtttctgacg ccggcctgcg tcgctttctt
    10681 ttccagatgg gaaaacagcg taaaatttgc cgcgcgcatc gaagcgactc ttcgcatggc
    10741 ggacactttc gattccaacc gattgaactc ggcggaaagt tctttgacct cggccagttt
    10801 tttctggttg ctgctgatgg atctgtccag tttcttttta gcttcccaga aggggaaaac
    10861 ggccagttcc agaatcagcg cgatacagac gaacacggcc gcgcccgcaa caagtttgcg
    10921 ctgtgtggca tccagtttgt tccacagttc ctgtatcatt ggacttcaat ccttaaactg
    10981 aaatcaactt tgccgccgtc acgggtcaga ctcgtcggcc ccatggtcac ctctttgaaa
    11041 gattttgatt tggccagcgc gtctttaatc aacgtcacat catcgaccgt tttcgcctct
    11101 ccttttaccg agatgaccct gttttcatac gtcagtgcag tgaagacgat ttccagggaa
    11161 ggcgatatca acgctgagag ctcctttaaa aaatcggctg ttgttgcttc cggcattcct
    11221 tcgccgagac cgaatgtttt tttatcttct tcgatttttg ttttcaattg ctgcaccgga
    11281 tccaccatgg acggagcgtc cggaaaactc ttttggaaaa tcctggcaat gtccttcttg
    11341 atgccgccaa tttgtcgcat tttcaggctg taatcgagaa tttgattcac cacggccagc
    11401 agcaggatca cggccgcaag gatgcccgcc cagcgcaggt gatttttgaa attcagatgc
    11461 gacggccggg cggtaaattc accctggcgg aaattgaatg attttttgcc ggccgaactt
    11521 ctcagcgcgt tggccgcggc cgcattcaat aaagacggat tcatccgcat catgatattt
    11581 ttttcaactt ccagttgtct ggcactgatc aggttgagaa gttccaccgg gcaggtaaag
    11641 tttttctcga tctctttgcg cagcggaccg aacagagccc cgccgcccgt cagcaggatt
    11701 tgtgcaggat cattgtgcag aacgccgttt atcttcatga attcgacggt gtttttcaat
    11761 tccgaacaaa gcccccggca ttcctccccg tccttttcca tagcctcggg ataatcgccg
    11821 ctgattttca gctgttccgc gcgattcctg tccacggaaa aatcttttgc catcgccagc
    11881 gtgatttgtt cgccgctgaa ggccagagaa cggatatgaa tcacagattc attttcgcaa
    11941 aatgcggctg tggtcgcatt gcgtccgata tcgagaatga tgctgacgga agttttttta
    12001 tgatccggta tctgtacggc cagcgccgaa gaagaggtgt caataaccga gacgtcgccg
    12061 agattttttt ccacctgttc aataatttcg cggatggttt ctttgggcat ggcgccgacg
    12121 agcagtcctt cgcggggaag ggcgatgtag tctgcaatga cgttttcaat gggcagagga
    12181 atgagcggtt cgagttcgaa gagcagcgtt ttgcggattc ggttatcatc gcggaagggc
    12241 aggcgaacat gacggaagag gacgtcctgg gaaggcacag acacaaagca ggggatacct
    12301 gcaaaatttt tatcttccgc caatttctgc agtgccggtc caattccttc gctgtcgcgc
    12361 acgtccagaa tgcgcgcgtc aagaatacga ccgccaccca agccttttcg ggccatcaga
    12421 acggctttaa ttgtgctccg gccgatttcc agtcccagaa cattcggcat taatcctgcc
    12481 tccacgtgat gatcgcaatc tgagcctggt cccccgtggc gacgatgccg gtcacggttc
    12541 ttgacatatt gctgattttg cccgtggata cgattttaaa ataagtgctt ttgaccgtaa
    12601 tcagattttg attaatggtt atgttttcca taccgggcac ctgtttgtac cattgatttc
    12661 cggaaacatc atggctcttc tccatgcggt attcatcgag cctgtcggcc agctcggaac
    12721 taatgtcatc gctgagagag cgtaaaacta ttttgggcgc cgtattgatg ttaatcaatc
    12781 cttcgctgtc tgcggtaaca tattttgcca atgatggttt gtctttcgtt ccataaaaga
    12841 tttctttggt tattcccttt accataagca attcttcaat gcaatcaaac ggagcgtttt
    12901 ttgcctgata gggagaatca agagattcgt agtaattgct ttccgctccg taaccggtcg
    12961 tgttctcatc tgtgtcgatc cagtctttga tggcatcgat gatttcatcg atttgtctgt
    13021 ccttcaggcc gaattccggc tgtgccagta accggtcgag catggttctg atttcttcat
    13081 tgtattcttc gccgtcaacc agcttgttca aagggatttt tcccgcttca tcttcgattc
    13141 tggcgataaa atacccttcc ggaaacagag tattggactg cagagatacc gtttccattt
    13201 tcgcccagtc gtcgcgcaga gaggtgtaag cgcccgggga attgttcaat agggcggcgg
    13261 ctccgtaaaa tcccgacttg gcgatatagc ttaattttat accgtcgctt aagttggctg
    13321 catcgtaaat atcggcgcgg gacgatctgt tcaactcgat caccacggca atcagaatga
    13381 caatgatcaa aatgaccgtg atcagggcaa cccctcgagg gttgtnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnngaaac aacaatttcc ggaacgcagt gaacggaaat
    13561 tggtaagttg aaccatcccg cgacagcagt gggttgtgaa acaacaattt ccggaacgca
    13621 gtgaacggaa attggtaagt tgaaccatcc cggcgttttc ggaaggccat gaaacctttg
    13681 aggatagaaa atttaagaaa tgacttttgg gggtcattct ttttttgatc atcctgatgt
    13741 ctggcgaata tgttcatcat tttatttgtt caccggtaag aatactctgg tcatgaattt
    13801 gtatggcttc tgcgcatcat catcgttgac cagcgcaagc tctatcttca ccgcttcggg
    13861 tactttgccc tgatgcatgc ctccaccggc ggacgtctcc catgtgtcat tttctccccc
    13921 ggccgaatcg tagaacgtca gatgaaatgt ttcaatgttt ttacagatga tgaatccgtc
    13981 ttctgttttt tttgtctcat ccggtttcat tccggtagcg tcggatctcc agagcgagaa
    14041 gctttttcca tcaggatttt ccgtcacgta atatccgatc gttgccggtc gcccctcacc
    14101 ttccagttct ccgaaagtca aatgggctgc cgaccagaaa aaaatgttgt gaaattgatg
    14161 ccgtcctgtt tttttctttt cggcgttcag gtagaacgat ccctcggaca tctgcagaga
    14221 cgttaaatcc ttcgccagcc gctccaggct ctggcgcgcc attttgtaga tttcgccttc
    14281 tttttccaac tggtgcgatg tttttagggt gccggtatag gaaacataaa cggtggccag
    14341 caccagagag agaatgaaga tagcgatgag aatttccacg agtgtgaatc cgccgccgct
    14401 taaatggggt ctggaagagc aatgtctcat agttacatat ccggcctttt atagaggatc
    14461 agtgtgtacg ttccgcggcg aagaaatagt ttgttggtga cggtgacctc gatttttttg
    14521 aattgaggca actgggtatc gccaatgatt aactgccagg catattgcgg atagtcgggg
    14581 ccgaaatcgc cgttttcggt tttattggat aaggggctct gtgtttcaac ctcgaccatt
    14641 ttgctctggg ccagaagtgc ggcggtcgtc atgaaacgcg aatccgtcgc catggaaatg
    14701 ctttgcgact gaagctgaaa gatggaaacc agcgcaatcg ccagaatggc catggcgacc
    14761 atgacttcca tcagagtaaa tcctttttct gtcattcgcg tcatagcctg gtcctcaccg
    14821 gcgattttgc ccttcggagg tgttgaaagg aatttccacg tactggtcgt aaatatcggt
    14881 gacacccata aagggattca tgacgatcgt cattttatct tccttgtaag ccagatgaat
    14941 cactctcggg gaacagatgt tatccttttt gaagacgatt tttacttcgc cggcggtaat
    15001 cttccggttg tcttcgtcca cgacatcgat aatgcggaca tcctcaggca aatgcagagc
    15061 ctgttttttg atttcagcct gtttgatctc tgtcatgtcg gaagccgtaa tccagtagac
    15121 ggagtcggat aaatccagat ggagaatata gtccagctga tcgcgaacgg cttccgttct
    15181 gagttttctt tccaggccga tcaactggcg cgacgttttc ttaagcatgt cgccggtgag
    15241 aacgtcccgt gtggacggaa cggccagcac cagaactatg ccgatcagcg tgataacaag
    15301 cagtaattca acaagcgtga atccctgctt actccacttc ccagttgttg atgtcctgat
    15361 cgacaccttc tcctccggct tcaccgtcct tgcccagaca ggataaatca aaatcgccgt
    15421 gactgccggg acttaaataa acaaaatcgt ttccccatgg gtctttgggc actttccctt
    15481 tttccagata tcctccctga cgccagttct tcgggagatc tcccgttgtc ggggcctcca
    15541 ccagagcctt cagcccctgt tccgtcgaag gataactgcc gttgtccagc ttgtagagtt
    15601 tcagcgccga ttccatggct tccatctgga ttcttgcctt ggcctgtctg gcttcgtcag
    15661 gacgtcccat gatgcgggga ataatcatac cggcaagaac acccaggatc acgatgacaa
    15721 ccatcagttc aatcagggtg aaccccgcct gtctgctttt ttttcttctg aaaattttca
    15781 taaaaccccc ttttttacgg atgtctaaat tatctgacca gctgattcat ttcgaaaatc
    15841 ggtaataaaa ttgagacaac gataaaaccc accaccagcc ccataaacag aatcatgatg
    15901 ggttccagca gcgacgtcat gaccgtgata ctggcctgtg attcgttttc ataagccgcg
    15961 gcaatacgat taagcatggt ctccagtgta ccgctttgtt ctccgacagc aatcatttcc
    16021 gtcaccagcg gtggaaacaa accgcttttg gtcaatggac cggacagccc tttgccttct
    16081 tcaagatctc tggccgcctg atgaatggca tcgccgatga taacattgtt gacaatgttc
    16141 cggacgattt ccatcgctgt cagaagcggc acgccgcttt gcaaaagcgt ggccagcgtt
    16201 cggcaaaacc gggcaatagc aattttgcgg ttgatttgtc ccatgaccgg aattttcagc
    16261 cggatgttat cccaggcgcg tctgccggaa tcggtgcccg ttgtcatata tctaatagcg
    16321 gcaagtccac cgaacaaaag gatcaggatg atccaccaga acgccttcag aaaattgctg
    16381 actttaatca gaacgatggt gaccaacggc aatgtctgat tcatatccgt aaaaattccg
    16441 gttatcttag ggacaacaaa cgtcatcaaa agaaagataa ccgcgctgcc catgataaac
    16501 atgatgatgg ggtaggctag ggcggaacgg attttattca tcagggcctg ctgattttca
    16561 ctgaagtcgg ccagccgctc taaaaccagg ttgatggttc ccgaagcctc gccggctctc
    16621 accatgttca gataaaaaga aggaaagatg cggggaaatt ccgccatggc ttccgtcagg
    16681 cttttgcctt cattgacctg ttcacggatt tgcgccaggg actttttcag aagcggattt
    16741 ttttcctgct gcatcagaat ggaaagcgac gggaccagag gcatgcctgc tcccagtaat
    16801 gtggaaagct ggcgggtaaa gacagccacc tggtttgccg aaaccctttc ccagagattt
    16861 atcttcagag ccccggccag agcggattct ttttttgttt ccgtctgatt gatttccacg
    16921 gggaaaatat tttcttcccg taacttttga cgcgctgcgg agatacctga agcatcgata
    16981 aatcccttca gttgtctccc tttttcatcc agcgcgatgt attcaaaaac actcattaat
    17041 ttttgctccg cctgccataa tgcctatggg cgcgtttaac ataaatttta ttacgattca
    17101 atcccgtgtc ttttgtttcg cattgtcccg actttctgtc acgtataata aaaaagggcc
    17161 taaaaagccc tttttatccg gaattgattt gcttaataat tcctttttat aaatcaggga
    17221 ttgcggaaac ttctcctgat tccgcgcttt ctccattgtt gtctacggct gtgacgacaa
    17281 aataatgcgt tttttcttta tcgagattcg cggcccgggt agacgtatcc gccagggcct
    17341 ctatcccttt ttgggctgtg gattttgtaa tgttgccggc ggtgaaaaaa tagacattat
    17401 aggttaatgc tcctgtgtgg aataatggag catcccacgc cagatcaacg aatccttcac
    17461 cgcgattgat gacccgaaga ttacggggcg cggatggagg cggatcttcg cttggcgttg
    17521 tcgtgtagac aaacgaagcg ctgctgtcgc cgctttcgtt gttgtcgtta ctgtcgactg
    17581 cggcaacgaa aaaaagatag gtttgtttgt tggtaagtcc tgtgaatgta tacgaggtgc
    17641 tcgtgatttt agggctttcc tgatagtttg caaaagacgt gtagtaatac acaatgtaac
    17701 cggccgcgcc ggttaccgca tcccacgtca cagtggcctc tttatcacct gcgttggcgc
    17761 ggacgttttt aggagcaggc aatggcggat tagcccgggg aatcgcataa atagcctggc
    17821 taaggtcgct ttccgcctgc tcggcatttt tggcggacag ggcaaaccag tacgttttcc
    17881 cgttttccag accggtaact acatacggcg cgctgatgat gtggtttgtt tctccatcta
    17941 tttttgtgcc ttcagatttc attttttgcg aggtgggttg attggtatcg tcttcgtcat
    18001 cttccatata ataaatgttg tatgtttcag ctctgggaag catctgccag tcaagcgaga
    18061 cttctccgtt gccggcatca gctctcattt gaaccgtcag atattccgcg tcggctgtat
    18121 cgtcaaaatc tgaatcgctg tccgagcagg cggaaacaaa aaagatcagc atactgatta
    18181 acagtaaata gaatagtgat tttttcattt atgctccttt tgaatcgtca taaaaaaccg
    18241 gaaagcacct ttggggacgg taaattttca gatgaatgag gactttcatt gccgacgctt
    18301 caggaatttt tcatgctatg attttaacaa ataatgacca ctaaagatta gcgctgtcaa
    18361 gttaattttt accgggtaac gtgttcatgt aggccgtgct gatatcatct gcattctcaa
    18421 tgcacaggct tcgcaaatag gctttcacaa atccttttaa aaagacgggt gccggcagtt
    18481 tttccgtttt gtcttcttca atgtcatgaa ggtaatcaag acggattttt gtttcctgat
    18541 ggatggtttc aattgccagc ccgagttctt ccctgatggc cttcaggtcg gcgccggtga
    18601 ttttctgccg ggaaagaatt tccctgatgg actggctttg ggaaattttt gtctttaatg
    18661 ccgatttgtt gtttttccgc gtcagggaag cgatatcccg gcggttaaat tcgaagatgt
    18721 tggccggttg ggggggggct ttgtgttggt ctgcggaaac ggacgtttcc cgcagattca
    18781 tttcgttgtc gtatttggct ctctgggctt cgttgatcag agtaaaataa gccttgtcga
    18841 tcagcgagag aattttatcg cgttctcctt tcgagaaaaa agaatagctg accagggaat
    18901 cgggctgata catttgcaac gcgacgttgt aggcgccgcg gatttccgca gatgtcgcgt
    18961 tcgtaccgat gtccaggatt tcgtaataat tcatctggtc aaaatctttt tccatgtgcc
    19021 gcctgattca attttttcag agatagttga gcatcaattg ttcaccgaag gcattgctca
    19081 ttttcctgtt taaaacacgc aaatcattgg cggcacgcgt gtgcggatac cggtccagaa
    19141 agcacacgcg ctggcagata gcgtcgtgaa cgtgttcatc gtaagcgatg ttgccggcaa
    19201 attccacatt gatgcccaga tgcttcttga taattttgca aatctgctcg cccagaacgg
    19261 tgttgtcctg tttgcgcagt tgattgagaa taagtttgaa acggaaagaa ttgaaatcct
    19321 ttctgatcaa cgcgtatttt tccggaaatt ctttcttgat gatcgaaacg atattttccg
    19381 gcttgttgaa tgactcctcg cccaggtgtt cccggacctt tttttcgagc gtcctgaagt
    19441 cggcagccga aaaataatgg cggatgcgac gcagatagat ggcgcgcatc agacggtaca
    19501 cgttttcaat ggacgtgggt tccggcgtgg tgataaagat gccgtttctt gaggccagga
    19561 aaaaatccag cgtgttaaaa gcggtacctg cccccaaatc cagaataatg tagtcataat
    19621 ccaacttgac aatggccttg agagttttta tttttttttc atagggaagg ttggcgatgt
    19681 ccaggctgtc gtgggcgccg ctgatcagaa aaagattagg aaagggtgtt tgaagaacgg
    19741 tatcttccag atcatggatt tttttggaga caaaatcaga gaaacacttt tccgggtagg
    19801 gaacgtccac catcgtgtgc agatttgctg ctcccagatc aagatcaatc agcagcgttt
    19861 tttttccgga tttggccaga agacgcccca tcgaacttgc cacaaagctc ttgcctgatc
    19921 ctcctttgcc tcccccgatt ggccaaatat gcgccataaa tcatccaatg agtaatttat
    19981 gaaagccata cacgataaaa caatgatatt caagcaatta gttccttcat ctgacaattt
    20041 tccgggaaat taaaaaagtc gttgcccata acgtccgcat agtccgggag tcgtcaggcg
    20101 atcactgtgc gttgaatgat tccgcacgcg gcgtcatgga aagtttctcg ttcgttgaat
    20161 ggcctgatgt tgcggagggc acgggaatgt cccggagagc acgatgggga atacattcct
    20221 ttcttgactt ctctttcttt tattcataaa aaacaaacca tgatccatta cgaacctgaa
    20281 atcagagttg cccttctgca aaattacagc gaagctcata tcaccttgaa aggggatttt
    20341 tcatctgcgc atggccggat attggaaggt tgctataccg ttaccgttga tgatggttgg
    20401 gtcctgcttg ccgattcgtc aggaaaagaa attgtgcggc agaaagaaat ccttttgcaa
    20461 gcgggaagcc gttctttttt taccgtatcg gatgtaaaaa tcggcattga tttccactgg
    20521 cagcggactc aggagcaatc gtttcgcggc gatttgtttt tatccgcttc acgagatgct
    20581 tctttcaatc tcatcaacaa tgttcatctg gaaaattatc tggagagtgt catctcttcg
    20641 gagatgtccg ccgaagcgcc tctggagttt ttgagagcac aggcaattac tgcgcgaagc
    20701 tggctggtgt cgatgcttgc caaaaagcag gaagcccgga catcaacacc tcagagaacc
    20761 ggaaatgaaa tcgttgtctg gcaggatgtc aacgatcatg atggttttga tgtgtgcgcc
    20821 gacgaccatt gccagcgcta tcagggaatc acaaaaataa tttcggaaaa tgtccataag
    20881 gcgatagaaa gtacccgcgg gcattttctc gtttatggag gaaaaatttg cgacgcgcgt
    20941 tattacaaat gctgcggcgg tcagacagat gttttcgata cggcgtggga agacaagcat
    21001 tatgattatt tgagcaatat cagcgatgac gtgaaaccgc attctcccgt gtgtacggaa
    21061 ggcgacgcca gacgatggtt gaccggcagg ccgagcgctt attgcaacac gagcgatgaa
    21121 aaattattga aacaaatcct gccggctttt gatcgggaaa cgtccgattt tttccgctgg
    21181 caggtcgcct atgagcggga agacctggaa gacatcatca gggaaaagtc agggattgat
    21241 ttcggagatc tgaaaaatat cgagccgctg gaacgcgggc cgtccggcag gatctcacgc
    21301 ctgaaaatcg acggctcgaa aaggacggtg atggtcggaa aggaactgga aattcgccgc
    21361 tggctttcac cgagtcatct attaagcagc gcttttgttg tgactgttcg gcgtaatacg
    21421 tatggagaag ttatcggttt tgttttcatc ggcgggggct gggggcacgg cgtggggctg
    21481 tgccagatcg gtgcggcggt gatggcctcc aagggatttc aagcggaaga aattctgacg
    21541 cactacttca ccggcgcgca gttaaaaaaa ctgtattgat aagccgtaat tgaattatca
    21601 gtgcgagtga gccgtccttc agatggtgtt gtagaataat attaagactt catcgcggca
    21661 tagctggact gtagctttca gttgaagtga cataaaaaag agaaaggaaa tgtccgaaac
    21721 aataacagaa acaacaagaa atgcggtccg gaggaatccc tggctttggg tgccttctct
    21781 ctatttagct gaaagcattc cctacacgat ggctatgaca gtcagcgttg tcctatacaa
    21841 agatatgggg ctttccaaca cgcagatcgc tttttacacc agttggcttt atctgccctg
    21901 ggtgattaaa ccgctctgga gtccgtttgt cgatattttc cgtaccaagc gcttctggat
    21961 actggcgatg caattggcta ttggcgcgtc gtttgccggt gtcgccctga cgctgccgac
    22021 aaccgatttt gtgcgctact ctctggctgt tttctggatt atggccttca gctccgccac
    22081 acacgatatc gccgccgacg gtttttacat gctgggactc gacacgccgc agcaggctgc
    22141 atttgtcggt gtgcgcgtta ttttttaccg tattgccact attgccgctg aaggcggtct
    22201 ggtgtttctg gccggtatgc tgcaaaaata cggttatccg gcagtcggcg cttggtcgct
    22261 atcctttatc gtcgtggcgg cggtttttct cgcgtttttt atttatcaca tatttgcatt
    22321 gccgaagccc gccgaagaca tcagcgcgcc gtgggacaaa tcaaaaaatt tccttgcgga
    22381 atatttccgc gttttgacct tattcttccg gcgcaaggat attctgatca tcatcacctt
    22441 ttttttgctt taccgttttg ccgaggcgca actgacgaaa atggtcgtgc cttttctgcg
    22501 cgatgaaatc agcaaagggg gattgggctt gagcacagcg gatatcggca tcatttacgg
    22561 tacggtcggc gtcattgcgc tgatgctggg cggtcttttg ggcggttatg tgatttacag
    22621 aaacggttta aaatattggc tgtggccgat ggttttcatc atgcacctgc cggatttgat
    22681 ttttgtgtat ttgtcccatt accagccgca gaacttatgg ctggtcggtt ccgccgtggc
    22741 ggtcgaaaaa ttcggttacg gattcggttt tacggcctac accatgtaca tgatcatggt
    22801 tgcacagggc agttacaaaa ccgtgtttta cgccatcggt acaggcatca tggcgctggg
    22861 catgatgatt ccggcgatgg gcagcggctg gattcaggat atgctcggct acaaaaattt
    22921 cttcatctgg attctgatta caaccattcc gggttttatt gttgcagcgc tggtgaagat
    22981 tgatcctgag tatgggaaga aaatcaacaa agaaccacgc gggtgaaact atatgcaaaa
    23041 caaacaactc aacacagcat ttattctagg ggcggggctg ggaacgaggc tcaggccgct
    23101 gacggaaaat acacccaaac cgcttctgga aatcggcgga catcccatga ttacttatgc
    23161 aatggcgcat ctgcgcgctg tcggtatcaa acggtttatt atcaacacgc atcactgcgc
    23221 ggaaaaatac gttgaggctt ttccggaagg caattggcag ggcactccga ttacgttttg
    23281 ccatgaaccg gttctgctcg acacggcagg cggcatcaaa aatatcgaag acctgattgc
    23341 cgaagatgaa cgaattatcg tttacaacgg cgacatcatt gccaacatgc cgatcgaact
    23401 gctgattaga aaacattttg agttaaaaac gtccgttacc ctggcgctgc gctcaacagg
    23461 tccgctgctc aatgtcagtg ttgataaaga tggctttgtc tgcgacatgc ggcatatttt
    23521 gaaaaatgat ggcgtcaaga aatgtttgtt tgccggtatt tatattgtgg aaaaatcttt
    23581 cctgaaacgg ttaacgacgg gaaaaatcga atcgattgtt ttaccgctgg tggagatgat
    23641 aaaggaaaat ccacgctctg tcggcggtgt tatcattgac gatggtggtt ggtatgatgt
    23701 tggtactgtc gctgaatatg aaaaattgag caaaacagat attcgttaaa tctcattcaa
    23761 aattgtcatg ccggcgaagg ccggtatcca gtatttttca gttttggatt agccgctcag
    23821 accgggtcat gacaacatta ttaagtgaag ctcatgcaga aggaaattca aaaattttta
    23881 actgaacatc tgggaaaaac cgattttaag ctggagtcga tcaagaaggg cggttcagac
    23941 cgtgaattct tcagggtgtc attgcctcac ggggacagtt ttattttcat gcgctacggc
    24001 gatgaggttg tggaaaatgc caactgggcc ggcatcaatc aattcatggc atctatggat
    24061 atcaatgtgc cgcggattat tctccgggat atccctcggc attttatttt gattgaggat
    24121 ctgggtgatg ttgatttgtg gtcgctcaga tttgaaccct gggaagaaag acgcaatcac
    24181 tattttcagg tgttggctca aattcatcgt ctgcattctt ttgatctgaa atccacttcg
    24241 cccgatgtgc aattatccga aagttacggt ccaaggcttt acaaatggga gcataactat
    24301 ttcctggaaa atctcgcatg ggaagtctgc aaaataaaac taacatctgc ggatgccgcg
    24361 aaactgaata aagagctgga cgcgcttttc gagagcctgc aaaaaataga accgtcgctg
    24421 attcaccgcg atttccagtc gcaaaacatc atgattaaaa atgaaaggcc tgtattgatt
    24481 gattttcagg gcatgcgtca gggctgtctt ttttacgatc tcggatctct tctctgcgat
    24541 ccatacgtca cctttgaaga gaaagaacga gatgagttgc tggattttta ttatgacatt
    24601 attcgtcccg cctatagccg cgatgagttc gctcataatt tctgggcggg ttccgtacag
    24661 cggcttttac aggcgctggg ggcgtacgga tatctgggac tcaaaaaaca caagcccgca
    24721 tttcttcacc acattcccaa cgggctggag aatttattga cggcagtgga caacgcgggg
    24781 gggcttaaaa tgcttaatga cttagcgacg gaatgcgaaa caatcattgc ccgtagaaat
    24841 atttagaatt aatctactat tttttagaag aacatgtccg caggacgaca aaaaagattt
    24901 ttttatcaat ccattcatgc ctattcatat tttcgtctgt tcagctccgc gtgatatgcg
    24961 gcaatcagat cgtcgtgcct taaaactccc agcacccggt tgggatcgtc ggaactcacc
    25021 accagcaatt cctctacatt ggtttccatg aattttacaa acgcgtcata aaaattgtct
    25081 tccggcgtta cggtttgcgg tggtgccatc aggtcctgcg caatgactaa atcttccagg
    25141 ccttcttcaa agacgatgga ccgggcgtgg tcccaattga tcgccccgat cagcacgtca
    25201 tcttcattgt acacgggaaa acattcgaca ttggatgcag aaaaaattct cttcagctgt
    25261 ccgtaagtcg tgatttcacg gacaggggtg accttctccg gcttgtagaa gtcctttacc
    25321 tgggtttcgg caaaaaggtt cacggtgaat tccgccatat gcgcgggcga ttcacgcctg
    25381 ctttgaacct gctttgtata gatggagtgc tgccgtgtaa aaagaatggc aatgatggac
    25441 accagcatca acggtgcaat gaggccgtat cctcgggtca tttccgaaca cattaacagt
    25501 gttccgatgg gcgcactggc cacccccgcg aagaatgacg ccatgcccac aaccacaaag
    25561 gcttccggct gcgtcacaat atgcggataa aaataatggg cggaaaatcc tacagtgccg
    25621 ccgatcattc ctccgataaa cagtgtcggg ccgaaaacac cgccggaccc accggacgat
    25681 attgtaaaac tggtggcgac aattttggcc aaggcgatcg ctatcatgat tccgatgctg
    25741 agtttcccca agatggccag ttggatccag ccccagccgt cgccgtaaac ctgaggaaag
    25801 acaaggccga tgcagcccac aaccagaccg cccagagccg gcttgacata gcggggaatc
    25861 ttcaggggca ggaaaatacg ctcccgcacc gcgtaaaata tcttgatgaa aaaaatcccg
    25921 gcggggacac agatcagacc cagaagcaca taaaagatca gctcgcgcgg attggaaaac
    25981 gtatactccg gaaaaaagaa aatcggcgtg ttgccaaaaa aatacgtaaa aatgatgtag
    26041 gcggtaatgg aacttaccac cgtgggcaca agggcttccg tttccatgtc ttccttgtaa
    26101 aggatttcaa tggccgtgag tgcgccgcct aaaggagcac ggaaaatcgc cgatagacct
    26161 cctccgcagc ccgccagaag aaggatgcgg cgctcgcggt tgctcaattt taaaagacgc
    26221 gcaacggccg aaccgatgcc ggcgccgatc tgggcaatcg gtccttccct tcctgcactg
    26281 ccgccgctgg aaagcgtgat aatagaggcc aatcccttga taacgggcac gcgcgtccgg
    26341 atgcgccctt ttttattgtg aaacgcatca atcatggcgt ccgtgccgtg tccttctgct
    26401 tccggtgcaa aatgatacac gagtaagccg ctgatcaaac cacccagagc gggcagaagg
    26461 aagaaaatca ggggatgaaa ttgtagtatg ccggggacgt cgaaaacctg ttcacccgct
    26521 ggtgcgggaa cggcataacc cgccagcaga tgaaaacaaa gatgacggcc tgcctgcaga
    26581 agataataaa aagccgaagc tcccagaccc gacacaatac cgaccagcag accgaacacc
    26641 agccagcgcg tgatattttc cagaaaaaaa cttcgcaacc agccgacaat ttgtttaatt
    26701 ttatgcaaga ccatcaaatc acctgatggg agaaagtcat gttttcccgt agagattttt
    26761 tgcaacttat cccgaattat tctccgatgc aaacgaaaag tctgatcgat ttcccgccgg
    26821 accgtttcat aatatgcaca agacctttac acgatgatca ttttctttcg tctccggcat
    26881 tcatctatct gcggcggcgg ttctgcgcca aaagcggata tcacacgtca ttcgtgagga
    26941 tgtattcagg accggatagg caaaacggtg tgaatgacat gcggaagagt caaaagtttt
    27001 ggattcctat caagtaacgg cgtgtgaggc ggcctggatg tcttgtaagc agaaaggcgg
    27061 cagtcggaat cggatcatcc agagacctgg acaccgaagg atggcgcggt tgatccacca
    27121 gatgtttttt tgtcattcat ttgaaatcat gtcaacattt cttaactcag accgacgact
    27181 tttgcgaagc tcccgcccaa aagcttttcc cggattatga catctgcaaa catgccctcg
    27241 attctgcgct tgataaagcc gtaatcgtat tgatgatcgg gcccgtgaaa gccaaacgga
    27301 ccgtccgtgc cgaaaaaaag tctttccgca ccgaacatat ccaccgcctg gcgggtggct
    27361 ttcccgctgg tataagtggt ttgagaaaga tccatatata catttttcag gggcttgatt
    27421 tttttccacg tatccgagta acaggggaaa ccggcgtggg ccagaaccaa cttaaggtcc
    27481 ggcacttttt tgagcagagc ttcataattt ccttcttcgc cgaatcccgc gtgaatcaga
    27541 agaggcttgc cggttttggc cagcctctcg gcaaccggag cgagttccac gggtgcgaag
    27601 tgatgccaga acggatgcgc cttgacgccg ataaatcctt tttcatcttt atatttttca
    27661 tactcctcga cctgattatt tttgccccgg ggatttacaa aaatccagcc cagaaattta
    27721 tccggatact ttctgacggc attaaatacc ttttcattat ccggatccgt cgcaagggga
    27781 tatggctttc ccaaaatcct gacatcgcca tggtcggtaa aattggaaac aaatgctttc
    27841 cctatacttc gcgagaaaga attttccagt aagcgttgta aaagattgat aagaatgcga
    27901 ggaggctccg taaatggtcc gataatgctg cccatgagcg caaccttgtc gatgccggct
    27961 ttctgcattt cgccgatcag acgctcaact gtaaaaaccc tttcttccag atgataatga
    28021 cagtcaatga tcatgctttt tctccataat aaacaataaa ctgattttat ttattgattc
    28081 ggtttgccgt acatctcctc aatctgttgc tgatattttt cattgacgac ccggcgcttg
    28141 attttcatgg tcggcgtcag ttcgccggag gcaacatcaa aggcgcggcc gagaatcgta
    28201 aaatatttta tctgctcgta acgcgccaga gtggcattga catgatcgac ttccttcgtg
    28261 ataagcgcct tgatatccgg atgattggcc aggtcctcct gtgaagcgca ggtgatgcca
    28321 tgatccttgg catagcgttt caggctttca aaatccggga tgatgaaagc cgtcagatac
    28381 ttgcgtccgt cgccgatcag acaggcctga tcaatgtacg gtctttcaat cagggccact
    28441 tcaatcgggt gcggagccac attcttgccg ccggaggtga caaacagttc cttcttgcgg
    28501 tcggtgattt gcagaaagcc ctcctcgtcc gccacaccga tatcgccggt catgaaaaat
    28561 ccatcgggcg taaaggcatc cgcggtatcc ttttcccgtt tccagtagca ctggaacacc
    28621 tgcggccccc tgatgagaat ttcgccgtct tcggcgatgc gcatttccgt ctcgggaaca
    28681 acacgtccga cgaagcccgg cttcacccgc aaccggtaaa cgagcgtatt gtagcagagc
    28741 gaaagcagca gggacatgac cggattggca tacggcgatt tgccctgcga ctgccgcacc
    28801 accatcagat gcaatgtcag ggccataatc ctgtcataga gaaccttgag aaaaccggtg
    28861 ggtggtttct ccatcatgat ttccggcgcg ttgagattat tgatggcggt ggtttccgtc
    28921 aggccgtaac cttcaatcag ttggatgccg agcgctctgt aaaaaataca gatatctttg
    28981 gacagttttc cgccgccgga acaggccatc accagtttgt ccatgcacat ttcttttttg
    29041 agacggtcga aaaccaggct gttggccaag ccgaaaagca taagctgatg aggcggcatg
    29101 cgttttcccg tggccatgca gtctacataa attctgccct tgctcatggc ccagttatag
    29161 atcgcctgat agggtttctt ggccctggaa agggtggact gaacgatttc atacgtcttt
    29221 tcataaaggc ggggaatgct gttgattata ttcggacgaa cctcccgcag gtcacgggca
    29281 atggtctgat agctctcagc aaaaacaagt tcaccgccca ttttcagtcc cgcggtatga
    29341 taatcgctca ttcgtccgta gacatggcaa agcggcagat gaaccagcgc cttcaaatgc
    29401 aaatcttttt gggccgtcag cgccatgatt tcggagccgg aacactggtg catggaatga
    29461 atccagttgg attgcgttag gatcacgcct ttctgcttac cggtcgttcc ggacgtgtaa
    29521 ataatcgacg cgacatcttc gggaacgacg cttcttatcc cctcttctat cacgtccagt
    29581 ttcaccttgg gatcacccaa tgcaaatacc tccgggaagg tgtaaacgcc ttcaggtttt
    29641 tgaccgacat catcccaggg gctcatcata atcacgggca ttttggcttt gcctgccgtt
    29701 actttaagca cccgtctcgc tttttccgga gtggagacaa tcgccagaca ggactcgctg
    29761 tcctcgagca tgtaatccag ttcttcctcc gaaacggtcg gataaagagg aacgccgatg
    29821 gcgcggcagg cctgaacagc ctgatcagcg ataatccagc gcggacggct ttcggagtaa
    29881 atcacaacct tatccttttt ttttattccc agcgccatca gccctttcgc cagagcaatg
    29941 gcttcttcgc gtacctgttt ccatgtccgc gaactgaatt tatcgctggc gcgtccgtcg
    30001 gcttcgaaac ggcccgaaag aaaaacgaat cgatccccgt aacgcagagc ctgattgtga
    30061 aaaagtccgc aaagcgtgtc cgcacgatag ttcatcacaa acctccttta ataaaagaat
    30121 ttgtttccct ttcttgtgtg ccgctgaatc aaaaggtgtt tagggttgcc cgaccggctg
    30181 cccgggcaaa aaatttatct aaatttttgt cacacggccg taatcgagtt tctctatgcc
    30241 ggccgcagca ttcaccaggt gttcgcgcag tttcgtatcg tactgcaagg tgatggcaat
    30301 ctgctccata atcggagagc cgccgccgtg ataagacccg taacacataa atcccgtact
    30361 ggatgaacag gttgcttccg cgaacagcag ccagaatttg atctgatcgg ctatcgggat
    30421 tttggggttg cgtttgagat atttttccag aagcggtttc aattccggat tggcgagatc
    30481 cgcttcataa gggaatgttg aaggcaggcc gccagctatg ttgcagagca tttcctgttc
    30541 atcaaaaaca gcttctccgg tgagacatcg acctacgttg gcatagatgg aatgcggaat
    30601 ataactaccg ggaccatagg gcatctggcc gatgccggga acataaattt ccggtttgct
    30661 cttggcggac gccgtaaatc cggccgcgta acccagctcg gtaaccataa tgatcctgga
    30721 aaggatttcc cgcacatgat gcgctttcga tatcccattc atttttgccg ccagcgaagc
    30781 tgtgcccagc agcatgtcgc cgattgccgg tttgcagcct gagtaactgt gacgatggaa
    30841 aagcgcaaaa agcatggcta aagcaccgcc gtgtacatat tcgccacaga ggaaaacccg
    30901 ctcccacggt acaaacacat catcgaaaat cacgtaggaa tcggtataac cgatatcggg
    30961 gtttccggtt ttgaaatggg tccgcttgcg aattttgtga atatgaacaa cctgtttgac
    31021 gccatcgtaa tctgccggca ccgcaaacga caccgcgaat tgctgttcgt caggaccgag
    31081 aattctcgtc ggcacgacga taatttcatc agcaatggat gcctccgaaa tgtgtagctt
    31141 gcatccccga acgacgatcc cgtctttctt cttttccaca acacgcagat acatgtcggg
    31201 atcggtctgc tcggcgggac gtttaagacg ttcccctttc atgtcggttt gagcgcagca
    31261 gcccaccaga tcatttcttt gaaagttttc cagccatttg agaaagtttt tatgatactc
    31321 ggtgctgcca ttgttctgtt tgtccgcttc ataacttgcc gcattgaccc cgttgatggc
    31381 atctacgccc atgcagcgct gaatgcacat tctgccggtg cggcagagcg cacgtgtcat
    31441 atcctgtttt ctatgcaggt catcggagtt ttgatgaacg tgacaaaacc ggttgatggt
    31501 ttcgccggta agatgagact tggcggtgca cagatccttg aattcaggaa tatgcggagc
    31561 atcgaacgtg aatcccatga cattcaaggc gggaagctgt tcctcatcat cccgatcgat
    31621 tttactgccg ttgaaataaa gatttctttt cattttccgc aggcctttga tgtattgttc
    31681 ttttgtcctc atcagacgct cctttcggtc atgtcttgta tttgcggtat ataattatca
    31741 acgtttaaaa tatatgtata aatattaatt catgataatt aaatgtcaag aaatatttat
    31801 tgacattagt tattaggtga attaatatta ttaacataaa taataattga tatttggttg
    31861 agaggtgttt tgggcgatcc cggcataaaa gtccatgaat gtgcttgtgt tttgacatga
    31921 tctgttttag ataagcggca ctgaacggat aaggagaggc tatggccaga cacgatatca
    31981 aggaagcaag gaaagataat cttattgatg ccgccctgcg ggtatttgcc gagaaaggtt
    32041 attcagacac aaccatgacc gacattgcca ggaaggcagg cgtgtccacg cccgtgttgt
    32101 atgaatattt caagaccaaa gaagatttgc tttttgccat tcctgaaaag tttatggaag
    32161 agccgatacg catcatggaa tacgtggctc cctatcttcg cggagcggag gcaaaaatca
    32221 ggggcattgt gcaaggatat ctgaccgttt atgatgagaa tcctctctac gcttccctgg
    32281 tgatgctgga gctgaagacc aatagaaaat ttttaaaggc caaggcatac gggatggtca
    32341 gaaaagtggc tagagggctt ctggaggtta ttgaggaagg aattaaagat ggaacattca
    32401 agaaagatac cgacgctttc cttgtccggt ccatgctttt gggcaccatt gaacatctgt
    32461 gcatccggaa gctgctgctc ggtgaaccca aaaatctgcc tcaatacacc gatgcgattg
    32521 tgaacatcat cctgaacagc attaagaagg aatcaaggca attctccgtt aatctgaata
    32581 tcaatgattt actgtctccc aacatcaaaa cagagaatat cccggcaaat aaaggcacgt
    32641 ctgatccaaa aagaaaaaaa cgataaaaat gtttagaact cattatcggt taatccggga
    32701 tttaccgtca atacggtact acggattcag ttgggattta atcctgccgg cgatgatttc
    32761 gatcggttcg ctaaatagcg cctccaatga aaaccggtca ataaaccggt catcccattt
    32821 gagattgtat tcgtcgataa gattgcgaat gtcgttgtat ctgtatccca gcgcgtcaat
    32881 tttttcctga agagtcagtt tttcatcgaa tcgaacatgc agcagcagga gattgccaat
    32941 tccggcttcc tgcgttctca acgggatgat cagaatgggg gcgttgtccg tttttcccct
    33001 gcccagatag gcatgccccg tgctgacgat tgttcttttc acccccatca gcacggcagg
    33061 cttgtcggcg cgcgatttca ttccggccgc aaggcctcct ttcacgcgta cggcgatcag
    33121 cgagtcgtct gtcggattgc cctgctggtc cagagcgctg atgtcataaa tcgtgtatcc
    33181 cggaatcgac gcaatgacgg gctggatgcg cctgatcatc atgatatccc gatacgtcag
    33241 agttgcgatg tcaaaggtga gcgttttcag cagatcaaaa acaatgcctt ccggcggcct
    33301 ttcttttcgg ctcgtgccga cggtaacggt ctttgcctga tgccgtatgg cgtcgatggg
    33361 acgcgacaat tcgtcaatga ccgtgcccag ggttgtatcg agcaactcaa acggtgaaaa
    33421 gatgccctgg tcgttgacga aatccagagc catatccttc agcggcagtt tgcccgctgc
    33481 atatttcagc agaagcatca gatcggagag agttttgccc tccagagaac tgaaggcgcc
    33541 ctgttggcga cgggtgttga acgactggta gaacgtgttg atgattcctt tgaccgacac
    33601 atccgccgtc aggtcaaaaa cggacagttg ctcacgcgcc tttagcatta aaatatcatg
    33661 aagacgggct cggaaggccc ggaagatttg tgcttcctca tcaatggcgc aggccgcgta
    33721 gtagccccat aaatgcccgg ccatcgcgtt aagaataacg gggagcggcg gaaacgcaac
    33781 cggaacggcg atcacggcat cggcgacgcc ttgaaaacgt ttttcgcctt catcggcgat
    33841 gacaatcacg ttggctttgt gagctttgaa aatggccacg tccttgacga tgtcggcggt
    33901 gaccgattca ggagaccctg cggtgcacac cagaatcagc ggctcggcgg acaggtcgat
    33961 atgttttttg ttttcgacga tgtccgttga aatggttttg taacagagtt cgctgagttt
    34021 gatgcggatt tcatccgcgg cggccttgtt cggaccgctg cctgtaacgg cccagtattt
    34081 tttgccgatg gcatccttca cggtcgaggc caccgacgcg cagtcggacg cgatttggtt
    34141 catcaggagc ggtgccgatt cgagatttct caaacaggcg atgatatctt catccgtgca
    34201 ggtccgcaga ctctgggcaa aaaagagcgc cagcacctgt ccggcgacga tctgggcgta
    34261 aaaagccttg ctggaagcga cggacatttc aatatcccgt ccatcgcttg tgtagaacac
    34321 accatgagat ttggccgtga tgtccgattg ccggcggttg acaatcgaaa taacgtgggc
    34381 gccgcgttct ttggccatcg ccacggcgcg attggtatcg gttgttgttc ccgattgtgt
    34441 aatcggaatg accagcgtat cggacagatc atctttcaga ccgaagccgc tcaactccga
    34501 ggcaacgcgc gctgtgatat ggagcggcga tgatcccaga taaagggaaa aagcgtccgc
    34561 caccgcctga ccggcaacgg ccgccgttcc gtgtccgatg atcacgatat tcttgattct
    34621 gccttttttc aatccggcaa tgatgtcggc gggaacaata tcttcgccga ggttgaatcg
    34681 gacctgctga agcgccgctt ctgtcgcggt catgcggtat ttcccccgca gggttctttt
    34741 gacggacaga gacgactcgg aaatctcctt caggaagaag tgcggataat ctccgcgatc
    34801 aatatcgcgg gttgtgattt cggctgttcc gagcatattt tccgttaacg ccagaatttt
    34861 gccgtcgtag gaataggacg tgatgccgcg cacgcctgcc ggaccgtttt gatcaagaat
    34921 acaaatctgg ccgcctgctt cgccgtccat tttgagaaaa cgcggcataa cttccaccag
    34981 cccgtagagt tcggaggaaa acatatattg atcgtcactg acgccgatat acagcgattg
    35041 gccgcttccc ctgagcgcga gaaacatctt gcccggctcc agatggctcg tcatggcgat
    35101 ggcgtgagag ccggccaaat cattgacggc acggcggaag gcttcagtca gcgtatgccc
    35161 cgagtggaga aatttttcta tctgcagggg aatgattttt gtgtctgtcg tgatcggccg
    35221 ggcaatcatc tcgccttggg tttccagaaa ctgccgcaac ggcgtgtagt tgtccacatc
    35281 gccgttgaga acgacatgga tctggacatc gctttccgga tacccggtag aaggacaggc
    35341 aaaacggatc atggaatgat tattcaccgg atgacagttt tcttccgtga tggagccgac
    35401 ggaagcccag cgggtatgca tcagaacatt ttcacaggat gtctcaaggt cggcaaagca
    35461 ccttaaaatg cggtcgttgc gtatcgaatt tctcagctcg gcaacattcc gtcccagttt
    35521 cccgaccgtg gagaacgttt tatatgtgaa cgtgacgaca atgccggcat tcgtcccgcg
    35581 ggtgtttttt gtgacggaga cagacagatt gagaagatct cccggctgag ttcttttttc
    35641 atagtcggca agccatccct gttccgttat ccgttgaagc gctgctttca gatcgttttc
    35701 gctttccgga gcgaaagtca aatcaatccc cgcggaatcc cggcctctga cttccaggcg
    35761 gtccaaagcg tttaacgcca ggttcagttt ccggtatttt ctgaatgcgg ccggccggat
    35821 atcggcacgg tttccggcgc ctgtcagcgc cagaattttg ggcagattgg ccagaatgtc
    35881 cttctccagc gcccagccaa tatccttgag catcacgatg agactgttga cggattcgag
    35941 ctccgccgag gtgaggaacg ctgtctgctc atccagccga ttttcttttt ctgcgataaa
    36001 acgattcagg tcggcggcga aaacggacaa atcagaggag taatcgggat gaaaaaaaag
    36061 aaattcctgc gcctcttctt ttttgagttg cgcgacggta tctgccatgg cgttgacggt
    36121 ttcggtgaca tccggcagcc tggatggatt tttcacgttg gcggatttaa gttttttcca
    36181 cagggcgacc aacgtgagat cggcccgggt ttgcggcggc tgacttttca ggccgcaggt
    36241 catcaacccg gcaaacccgc aacataaata cccgtcaaga accgggaaaa aaatcagaga
    36301 aggtcttgcg acatggtcgg gacggcggcc gacatatata tggcagcgac cggcgaaatg
    36361 aacaacggtt ttaacaaggc gacgaatatt ttccgatatc ataagtcaac tattttccga
    36421 ttgctgatgc atctccaaaa aaacaaatca tcttctgtgt aacaatatcc gacaggagcc
    36481 gtaaatcctg gttgcgcata tcaaaaactt taaccacttt cgcaaagcgc ccgtcctccg
    36541 gcatcaggtc ggccagaatg ttgggctgtt tggccatcca cgacgtgttg aaaaaaacgc
    36601 cctctttttc gggataaatg gccatataaa aaatgtccat ctctaccata tcctggaaaa
    36661 aatgtgttcc aaaagataaa tccggaatca gcgcgccgtc ctggtacgca atttcgccga
    36721 tggccatgat attgttgatt tccgaaaagg ttaccggaac gcccatggcg ggcgtggtgg
    36781 tgccccatcg tccgggaccg aacagaattg tgggcatttt ctcccgcttg ccgatctgtt
    36841 tgttcagctt gcccgtaagt cgcgcgacct cgtgtttttc tgaaaccgtc agtctggcat
    36901 agcctctggg atcaatataa atgatgcggg atatggcttg ataaacgctt ccgcccatga
    36961 aattggcttc ctgccttaag aggattctgg acttttcgat tttttccggc agttccacgc
    37021 ggctgtagtg tcccttggtc tggaaggggc ggcactgcag aaggttgatt ttgggttttc
    37081 cctctgcatt gaagttggct gtaaattcga tgtctaccgg atagcggtaa acctgcgcca
    37141 gttttttaag catatcggac attgtcctgg caaaaggcgt attttccagc aattcatcaa
    37201 aggtcaatat ccaggtttct ttcgcttccc gtcccagcga ctgataatat tcgtcaacgg
    37261 ccgcatcgcg tacggcaatc ataccgatat ggggttcgat ggcatagtcc agcaggtcgg
    37321 aagtcggcag tgtctggaaa tgattgtctt tcaaattcag gacatccact tcgttctggg
    37381 aaaaccgcct gatatcctcc tgtttagcgt ggggtttgat cagcggtgcg tcgagcgcca
    37441 caatacgggg atagtcgttt tccacgcgat tgacggctct tgtgcccagg ccgaagacga
    37501 tccgcagcat tccggctttc ggatccatgc ctttttgcca gacgaacgtg ttataggaaa
    37561 ggcccacacc ggcaatgtcg gggaagaaat atctgttgcg atgtgatccg gaaacgcgct
    37621 gcaccagcag cgccatctgt tcgtcctgat gagccatgcc gcgttgaaca cgataggtca
    37681 gagcgtcctc attcatcgtg gatgcgaaaa ttttccttac ggcatcctca aagtttttat
    37741 atcgtgtttc aggcgtgccc tgattcgggc agaaatagct ttcgtatttt ccggcaaagg
    37801 cgttgccgaa ggcatcttcc agaagcgaac tggaccggac gataatcggc gactgcccga
    37861 aatattccag catgagctga aactgctctt tgatttcgtc ggggaaaatg ccgtgaagca
    37921 tcctgctttt aagctcccgg gcgacttcaa aatatccctc gtcggttttt tgcgccatca
    37981 gcagtttcca ccagccgttt tgcaccatat atgaataaaa aatgtccgag ccgatataaa
    38041 aggaatcgtg caactccagt tcggaatccc agtcgaaggc ggggtccttg cggagaatat
    38101 tgcgcgccat cagcataccg accgatttgc cgccgataaa ccccgtgccg atgaggcgtt
    38161 tcttgatttc cagcaaatct tccagtgaaa agtattcctt gaccagcgaa agcattcttt
    38221 tttcacgccc gatcattact ctcgaaagag aatcgaccat ttcctgtcta tcaaggacgg
    38281 cgtcggggtc ttcaagaaga ttactggcct ttaagaacag gcggtcccaa taatccagat
    38341 tgcgcacgcc gcccgtggtg tttttgttcg tgagatagga aagaaaactt gccgcatccg
    38401 cgctgtttaa tatcgggaca aatttatcat tttccatgat gtgcggcagg aacatggtcg
    38461 gcgtgtagcg tttccatgcc ttaagcgggt ggacgcacaa ctggccgttg ctgttgtgaa
    38521 tatccagcag cacctgcgtt gtttcgcgaa tccgcgctac ggttttaaaa gagtggctgc
    38581 tgcgcagaat ggcaaaatac gcgactgtgt ttagttcgaa cagataagga caggtgatca
    38641 caaaaaagtt tccgatcatc agatcggtgg cccaggccat caacagatcg gacaggcagt
    38701 cgaagacata aaacacatcc cgtccttctt ccttgataat gttgtgcacc tgtgtcgaaa
    38761 acgattcaaa cccgttgttg gcgttgagct tgtatacctt gatttttttg tttgcctcca
    38821 gcagaggcgg gtgagcggca aagcgcatgt aaaccagccg atgcctggaa gcaagagagg
    38881 ttttgacgaa cggctcgaca aattttttgt aatcctcgat gtcgtcaacc tgcaggacaa
    38941 cattatcccc catttgaaga ccgttgatga tgtcatcgag gctgctcgtt cctgtgctga
    39001 cgcgtgcata tggtgtcata aatctttccc tcatccatta ttcggaacaa atttgtacca
    39061 taaataatcg ttccattttt atttcttctt gtcctaaacg gcgcctcttt tcaaaagaat
    39121 aataatttca agtattttca tcagcacaat gacgtttatc ggtgcatccg gcggtgtttg
    39181 atgaaaaatc agtttctgct tcctgataga tgaatcaatt tttgtgccag aatatttttc
    39241 ttcttgcatg gaacgaactg cattcgggag ggggaggtag aaattaaaaa tctttggacg
    39301 gcaattttgc ggaagaaaca tttctgaaac gttttttgag aagccggatt gtttttaaac
    39361 gggagaagtg tcaacatttc aggactcaaa aaccatgatt tatacagtat tgtatcaatt
    39421 tatcagcctc aaaattttcg tattaataaa taataccaat aacttatcac aataaaaaca
    39481 ttttggcatt gtccttgcca taatacagac gaaggataaa ttcatcagga ggtattgatt
    39541 atggattctg gaaatacagc gtgggttctg acagccagcg ccctggtttt gttaatgacc
    39601 attccggggc tggcattttt ttacggagga ctggttcgcc gcaagaacgt cctctccatt
    39661 atgatgcaat gttttgtcat tgtttgtgta atcagtctgc agtggatgct gttcggttat
    39721 tcgctggcat tcggcccgga ttttcacggc atcatcggta aactggactg gatggggctg
    39781 gccggagtgg gcgcaacgcc gaatccggat tattccggcg gcattcctca ttacgccttt
    39841 atgatttttc aggccatgtt tgcgatcatt acgccggcat taatcatcgg cgcttacgcg
    39901 gaacgcgtga aattttccgc cttcctggtc tttaccttat tatgggcgac ctttgtttat
    39961 gatccgctgg cccactgggt ctggggtgtc ggcggctgga tgagaacgct gggcgccctg
    40021 gattttgccg ggggaattgt cgtgcatgtc agttcgggaa tttctgcttt agtgctggct
    40081 ttaatgctgg gcaaaagaat cggctacaac cataaaccca ttcggccgca taatctgccc
    40141 tttaccgttc tgggcgccgc tctattgtgg gttggctggt tcggcttcaa cgcgggcagc
    40201 gccctggccg ccgatggcct ggccgccaat gcttttgtga ccacacatat cgccacggct
    40261 gccgccggca tcacctgggc tttgattgaa tggtggcaca acggcacgcc gaccattttg
    40321 ggcgcggcaa caggggccgt ggccggtctc gttgccgtca cgcccgcctg cggttttgtc
    40381 aatccgatga atgccatgtt tatcggcatg atcgtcgccg tcgtctgcta tatcgcggtt
    40441 gcggtcatca agggcaaact cggctacgat gactctctgg atgccttcgg cgtgcacggc
    40501 gtgggcggaa ccgtcggaac catcgccacc ggattgttcg cggaaaaagt tgtcaacgcc
    40561 gccggagccg acggtctgct tttcggcaat gcccatcagc tttacgttca gtctctgtcg
    40621 ctgattgtca ccgtcgcctt tgccgcgatc atgaccttca tcatcttcaa gctcgtggat
    40681 gcgctcatcg gcatgcgtgt ggaagagaat aatgaagtcg ttggtctgga tttaacgcaa
    40741 caaagcgaag ccgcttacac cgtgatcgaa taggaggaat gcattatgaa attagtcata
    40801 gccattattc agccgcacaa actggaagcg gtgaaaagag aactggaggc cgtggacgtc
    40861 aatctgatga ccgtcaccaa tgttctggga cagggccggc aaaaaggcgt cacggaaatt
    40921 taccgcggcg cgaaggaagc cggaggcctg ctcaataaag tcaggctgga tatcgcggtc
    40981 aatgaagatt ttgtcgaacc gaccgttaaa gcgatcacga aaggggccca taccggctcg
    41041 gtcggcgacg gaaagatctt tatcgtcgaa cttgccgagt gcataagaat cagcagcggt
    41101 gaacgcggtg gcttagccat cggttgaggc ggccgctatg agcatacgca tccataaagc
    41161 caaaacaaac gccctggccc gttgcgtttc cgaatgcgaa aaagcggtga tcatgcacgt
    41221 tctgctcaaa acgaaaggcg atcaatcgga agcggcttca cttttgggaa ttaaggaaag
    41281 ttttctggcg gataaaattc atcaatatga aattgattgc gcccgattcg aattaaaata
    41341 attgtaaagg agaatgaaaa agatgaaaga caatttaaaa actggaataa tcatggtttt
    41401 gctgactctg atcctggcat tgctgcccgt gatgagttgg gcggatgaag aaatagttcc
    41461 tgccgatccg aaacagggag ataacgcatc aacggaagtc aaacctgtgg agcaagatca
    41521 gccggaagat aaagtgaccg gcgaggttgc ggcaagcgtt ttgagcgcct atatctggcg
    41581 cggacaggaa ttgacccgtc acagtgtggt cattcaaccc tccgttaccg ccgcttataa
    41641 aggctttgcc gccaatgtct ggggaaatct tgatacaaaa ccgtactctg ctctcgatgt
    41701 ggattattca tccaacttca ccgagacgga tgttaccctg tcctatttgc ataaattcgg
    41761 gatcttgcag gcgggcatag gttacatata ttacggcctg gcaggagccg ttcagggcgg
    41821 cgccgatctt ccggattcac aggaagtgtt tgtaatgctt ggacttgaca cgattcttgc
    41881 tccgacgctg actgtttata aagagttcga ttactacaat cagtggtatg ctacgctggg
    41941 aatttctcat acttttgcgt tgcatgaaaa agttggtctg aaatttgctg ctcaagcaag
    42001 ttatttgaga agcgacgatg aggcaacgta tccggaattt gacagcgctt cactggccac
    42061 gaccgataaa tttaataatt tgcacgacgg tgttgtctcc gtttctctgc cgattgccgt
    42121 aaccggccat ctgacaatca gtcccgtgct gacctacgtc tttccgcttt ccgatgatgc
    42181 gaaatacgag atgaaagccc gcggactgaa aggagacatc gatccctccc ataaggaagg
    42241 cgattttctg tatggcggca ttgtagtgag ttacgcattc taaatttaaa tgccgatgcg
    42301 ttttggtaga tgatctttga atggattttg ctgatgggtc gtctaaaggt aggacaacag
    42361 gttctggccc tgtgaatcga ggttcgagtc cttgcccatc agccatttgc agcaaattta
    42421 ctgagatact aaaaatttca atgacaatcg gagaaaaaaa ctttcatgcg cgcgcactac
    42481 tttcagcatg ttcctttcga agggctgggc agcatcgagt cctggctgaa aaaatccggt
    42541 tttaaaataa ccagcaccag attctatgaa tcggccaaac tccccgatct taaaacgata
    42601 gatcttcttg tagtgatggg cggtccgatg agcgtcaacg acgaagatcg attcccctgg
    42661 ctggttgccg aaaaacaatt catctgggat gccatcgcta aaagtaaacc ggtgctggga
    42721 atctgcctgg gcgcgcagct tattgccagc gcgttgggcg ccggagtgta tcccaataaa
    42781 gtaaaagaga tcggctggtt tccgatccat gggattgaat caaacggcca atccgttttt
    42841 gcgtttccgg agtccatcag cgtattccac tggcacgggg agacgtttga tttgccgtcg
    42901 ggagcaacgc ttctggcaaa aagcgacgcc tgcgaacatc aggcgtttca ggtcggcaag
    42961 tcggttatcg gtcttcagtt tcacttggaa acgatgcccg cagcagcaca ggcgcttgtt
    43021 accaactgcc gcgatgaatt ggtcccggcg cccttcattc agaccgaaga agaaatactg
    43081 tcgacaactc ctgatcaata cacagccatc aatgatctga tggccgacat tttatctttt
    43141 ctgcttacac aaaaaatgac ggcaggactg taatcgagtt gtcctgtatc aaatactcat
    43201 aataaatatc tttacgtatt tatgatcttg cccggttcag cagcggcgat aatttcgcat
    43261 tctttctgca tttttttaca aaggtgtaat gatttggcgc attatttgcg atagagagat
    43321 tttaaagaaa aaacattggt tgaaatttga acaattttgt aacaaaataa attgctggcg
    43381 aaataaagat gactaaaacg tcaaaaaaat ctttggaaga gagagaaagt caattgagta
    43441 ttcataccta tgaggaattg gcagccctgc atgcgatagc gcaaatttta gcccaacccg
    43501 gagatttaca ccatcagctt gcactgattt taaaggaaat gagttctcgt ctcgggatgc
    43561 agcgcgggat gatatcgctc gttgaccgcg ataataagga atcgtggctg gatgtggccc
    43621 atgatgtgaa tatagatggc cttgagataa cctataaaca tggcgaaggc attaccggaa
    43681 aagttgcaga aaccggccgg cccatggcgg tggccaatct gggtcaggaa acgcatttcc
    43741 tggaccgcac cggtgcccgc cgtcatttga atcgcgcgga gctttccttt ttatgcgtgc
    43801 cgattatata tgatttcatg gtggaggggg ttctctcggc cgataaagtg gccgtcaaag
    43861 tcgaaaatct ggataaggaa ctcgccttga tggcatccgt cgccgaactg atggccaagg
    43921 ccgttcatat ccgcgctctt gaggaggaaa acaaacgttt gcgtaaaatc atcggcagca
    43981 cccgagcgcc tttgatggag attatcggac attccaaggc gatgcaggaa gtcttcgggc
    44041 tggtggcgca ggtggccgat tccaacacca ccgttttgat caccggagaa acgggaacgg
    44101 gcaaggaatt aatcgcccgt gccattcata aaaactcagt gcgtaagaac ggtcccatgg
    44161 tccaggtcaa ttgcgcggct attccggata ccctgattga aagtgaattg ttcggtcatg
    44221 aaaaaggggc gttcaccgga gccttgacgc agcgccgcgg gcgctttgag gaagccaacg
    44281 gcggcacaat ctttcttgac gaggtcgggg aactttcagc cgccgcccag gtcaaactgc
    44341 tgcgggtttt gcaggaaaaa cgttttcaac cgctggggtc gtcccgtgtg gttaatgtca
    44401 atgtccggat tattgccgcg accaaccgca atctggagca ggatatcctt tccgaccgct
    44461 tccgggcgga cctgttttac cggctgaatg tatttcccat ttatttgcct cctcttcgcg
    44521 agcgcggcag cgatgtgatt atgctggccg accactttct tctgaagtac agtaaagaga
    44581 tgggcaaaca ggtgaaacgg atttcccctg cggctatcga ggtttttctt tcccataaat
    44641 ggccgggcaa tgtccgcgaa ctggaaaact gtattgaacg gggtgttctg atgaccaaga
    44701 cggatgttct ggattgcgag catctgcccc cgtccctgca attgaaagac ggaatgcatg
    44761 atcgcaaagg tcgcggcaaa ctgtcttccg ttgtggaggc tcaggagaga ttgctgatca
    44821 ttgacgcgct taaagaaaca agcggtaatc agaccaaatc ggccaaactg ttgggtacga
    44881 ccaagcggat tatccagtac aaaatttcca agatggggat tgatcccaaa aattaccgta
    44941 aaaaaagcta aagatggcct tcccctgcca tgacctctct acatttttcg gtgcaaatcg
    45001 aacaaaaatg tttcactttt ctatctaaga acaaaaatgt cacttccatt gttttatctg
    45061 tacggcaatt ctccataaaa aatacctaaa agttgattaa ttcaggacat tttaattttc
    45121 aacattgatg gcacgacttt agcagttagc caaggcggtc caagtgtgct ctttttgtac
    45181 caaggaggaa agaaaatatg tgtcgcctgt ttggcatgac aagtaatgat ccggtttccc
    45241 cgctggcagc cattcgcgcc atcgatgtga tgagggaagg gcatgacggt tcgggtgtcg
    45301 ggttatttct gacggatctg ggaagcgatt tccaggaatt caaaaacgag ccgattcttt
    45361 ccgggatttt ttccaatgaa ggactcaaaa cgctcgaccg attcatgatc gatcatgatt
    45421 ttatggtgaa atacaaactt tccatcagac cggcaaagaa tcctcccgcg ggaacgccca
    45481 aacgcgataa ttacgttatc cggatttatg aatatcccga ggagtgggac ggattatgcc
    45541 gggacgaaat cgaatttcgg ctgatgatgg ttcaattgga attgcggcgg atgggcgagc
    45601 aggacgaatc gatgctggtg ttcagtttct ggcccgatgt gatcattatc aaagaggttg
    45661 gagatccgct cgctgttgcc gaatatctcg gactggaccg caaagagctg atggcccgcg
    45721 ttatcctgag ccagggacga cagaatacaa actatgcgat caatatctat gcctgccatc
    45781 cgttttttat tcaggggatg gcgactgtga caaatggaga aaacaccgct tttgttccga
    45841 tacgggaatt tctgatgtcc cgcaaattcc cgggttatac cggttatcat tcggactcgg
    45901 aaacgttcac gcatattctg cattacatgt atcatcaatt gggtatgggc atggaaatgt
    45961 acaagcatat cattacaccg cttaaggacg gggaactgaa ccgtcatccc gacggcgtgt
    46021 ggctcagaaa attgaaacag tcctgccgcc cgctgattat tgacggtccc aattgcgtca
    46081 tcggatgtct gccggataaa tcgatgttca tggtgcagga ttcgaaaaaa cttcgtcctg
    46141 gcgtggtggg cggtcgtccc ggggtgttcg cgttttcttc ggaaatgtgc ggcctcgacg
    46201 cggcccttcc ggacagatat gttaacctgg attttcaacc catgaaatat gaaacagtga
    46261 tcgttcgcag cgaacggcag gagatcgaaa aatggaatca gctggaaccg ttaacccatc
    46321 cacattaagc gtcaaagatc tgccctggca gatcatgtgg aacaaagaga aatgcaccct
    46381 gtgcggccgg tgcaccgcgg tgtgtccggt gcgtgcgatc gaaccgggcg tgcatcgcaa
    46441 acgcgcgctg caggttcctg tcggtctgga agaaaaaccc gcccagttgt tgtccgttta
    46501 tttcgggatt gatcagcgca ccgatgcggc gcatgcctgc gtcggttgcg gcatgtgcac
    46561 tctggtttgt cccaacggcg ccatcatgcc ggtgcgtgcg gaagaaacag acaaactgcg
    46621 gtttcacgtc aaccagggcg gcgagccccg caggcgcggc ggacggcgca atgttccgga
    46681 cagcgttctg gataaaataa aatttatccg gatttctatg ctgaccgatc cggcgctgga
    46741 tgcgggacgt catgaatttg aactcaggac cctgctgggc agagtgcttc cgccggagga
    46801 gcttttgaaa gtccatcgtg aaaacggatg ggtgccgccg gtgcgtgaaa tatatccgtt
    46861 ggttatcggc gggatgtctt tcggggcatt gtccccgaat atgtgggaag gcctgcagat
    46921 gggggtggcg tatcttaatg aagaactggg gatgcccgtg cgcatctgca ccggagaggg
    46981 gggatgccct ccgcgactcc tgaggtcccg ttttttgaaa tatgtcattc tgcaaattgc
    47041 cagtggctat ttcgggtggg atgaaatcat tcatggtctt ccgcagatga aagaagatcc
    47101 ctgcgcgatt gaaataaaat acggccaggg cgccaagccc ggcgacggcg gattgctgat
    47161 gtggtacaag gtcaataaac tgatcgccgc catccgcggc gtaccgcagg gcgtgagcct
    47221 gcccagcccg ccgacacatc agaccaagta ctcgatcgag gaagccgtgg ccaagatgat
    47281 tcagtccatg tccatggcct ggggtttccg cgtgccggtc tatccgaaaa tatccgcgac
    47341 cagcacgacg cttgccgtgc tgaataatct gaccaggaac ccttatgccg ccgcgctggc
    47401 cattgacgga gaagacggcg gaacaggcgc tgcttataat gtctccatga atcatatggg
    47461 gcatccgatc gccagcaatt tgcgcgacgc gtatctgaat ttggtcaaga tcggcaagca
    47521 aaatgaagtc cctctgtttg ccggaggcgg catcggcaag aacggcaatc tcgcggccaa
    47581 tgccgccgcc ctgatcatgc tgggtgccag cggtgttcaa accggaaaat atatcatgca
    47641 ggcggcggcc ggttgtctgg ggtccgagac ggaccgctgt aatatttgca atatcggcgc
    47701 ctgtccgaaa ggcattacct cgcaggattc gcgactctat cggagactgg atcccgaaaa
    47761 ggtcgctcaa cggctcgtcg atgtattttt gagctttgat acggagttga aaaaaatcgt
    47821 cgcaccgctg gggcgttcga catctctgcc gatcggcatg tccgatgcgc tggggattgc
    47881 cgatgccggc gcggctgaga gattaaaaat aaaatacgtt ctgtagaatg aaaacagtgc
    47941 agggattatg acaaagaaag aatcgttaaa agtaaccggc caggaaaacg gaatccgtgt
    48001 tgaatcgcga attctcgaag agcggattca gcaggctgta gccgagggct atcgtcatat
    48061 tgagataacg gcttacggcc agcatggtat cggcggccgt ctctggcggg ccggagaaac
    48121 gccaatcaag gtggatgtga cgggaacatc gggacagcgt ctcggttcca tggggtttgc
    48181 caacacgctc atcgaggtgt tcggtccggt ttccgatgac gtgggctggc tcaatgccgg
    48241 agcgaaaatc attgtccacg gtaatgccac caacggggtg gccaacgcca tggcccaggg
    48301 gaaagttttt attgacggcg atatcggcgc ccggggcatg accatgacca aacacaatcc
    48361 gcgttttgat ccgccggaac tctgggtgtt cggaaaagcc ggcgattcgt tcgcggaatt
    48421 catggccggc ggcatcgcgg tcatctgcgg tgtcggcgct gaaaatgagg atgacgtttt
    48481 gggttaccgt ccctgcgtgg ggatggtggg aggcaagatt ttcttccgcg gccgtcaaag
    48541 ctcttacagc acccaggatg cgcgactcat taccgatctt ccggatgagg aatggcggtg
    48601 gctgacaaaa aatctgaaag cctttctgca ggatgtcggc aaacaatact tatgtgaaga
    48661 actgaccgcc cgtcgttcgg actggaagct tctgtccgct ttgaaacccc acgagaagaa
    48721 agggcgtgcc ctgagtccga tgacggttta ccggaaagag gcatgggaaa aggaactggg
    48781 caccggcggt ctgatcggtg acttgaccgg cgccgaccag tccccgatcg gtctgatcac
    48841 gaccggtgat ttacgccgtt ttgttcccgt ctgggccaat gaaaaatatc tgccgccctg
    48901 tcaggcgagc tgtccgacgg gcattcccgt tcaaaaacga tgggaattga tccgcaaagg
    48961 aaaaatgaaa gacgcggtgg atcttgccct gcggtatacg cctttcccgg cgacggtttg
    49021 cggttatctt tgccccaatc tttgcatgca gaactgttcg cgaaaaatcg tcagcctgcc
    49081 gtcggtggat gtaaccgttc tggggaaagc gtctctggaa gcaaaagaac cgaaaccact
    49141 gccggccacc ggtaaaaaaa tagcgatcat cggcggcggc gccgcgggac tttccgtggc
    49201 ctggcagtta tggatgaacg gtcatgacgc ggtgattttc gagagcagaa aacatctggg
    49261 cggtaaaatt acccaaacca ttccgcagag ccgtattccc gataatgttg tggaacatga
    49321 gctcaaacgg ctggcgaaaa atctgcgtca cattcatctg aacgcgtctt tgacgaaaga
    49381 gaagtttaga aaattgaaga atgaattcga cttcatcgtc attgccgccg gagcggcaaa
    49441 accccggaaa ctaaatgtcc ccggaatgga aaaagcattg accgcgctcg agtttctgaa
    49501 acagagcaaa ggcaacggca tcaatgtcgg aaagaaagtc gtcatcgttg gcgccggcaa
    49561 tgtcggatgt gacgccgcga cggaagcttt tcgcctgggc gccaaaaccg ttacgctgat
    49621 tgacgttcag cagccggcat ccttcggcac ggaacggaaa catgccgaag cggcgggtgc
    49681 gatcttcctc tggccccgct ttaccaaggc catcacggcc agaggcgtgg aactgaccga
    49741 cggggaaatc ctgcctgcgg atacggtcat cgtggctgtc ggcgacgttc cggatatatc
    49801 gtttttgccg gaagaaatag ccgtcaagaa cgggttcatc gcggtggacg aacaattcag
    49861 aacaagcgac gatcagatat ttgccgtcgg cgatgtcacc aagcccggtt tgctcactga
    49921 agccatcggc gcgggaagaa tcgccgccgg aacaattgac gcattgctca gaggcgccgg
    49981 tgaaacatat gacaaactgc cgccgatcga tgttcaacgg gtgaagacag aatatttcga
    50041 ttcgcgcatc aaggcttttt ccgatcccgt ttcctgcgcc gtgaactgcg cgtcctgcgg
    50101 cgcctgccgt gactgcggta tgtgcgagac catctgtccg cagaatgcca tttcgcgcaa
    50161 agccctaaac ggtgaaaatt atgagtatgt tgttgatgcg gaactttgca tggggtgcgg
    50221 tttttgcgcc ggcgtttgtc cgaccggcgt ctgggaaatc gtggacaata aaccactcga
    50281 gtaaaaaaat ccaatagcga ggataagaaa gactatgaaa aaaacaaaaa agaccactgc
    50341 agcggagcgg gaatacgtta ttgaaaagga gaaattcgtt ccggtttcaa agtatttcgg
    50401 cgaggatacc ttcaaccaaa aaacgatgaa agagcgtctg ccgaaagaaa ccttcaaaaa
    50461 actgatggac gccatcaacg aagacaagcg gctggattta aagacagccg atgtcgtggc
    50521 tgaggccatg aagaaatggg ctctggaaaa aggg
//
LOCUS       DAONQZ010000011        49171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_763_length_49171_cov_13.898119, whole genome shotgun sequence.
ACCESSION   DAONQZ010000011 DAONQZ010000000
VERSION     DAONQZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 49171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49171
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_763_length_49171_cov_13.898119"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="lsrF"
                     /locus_tag="PLR13_03510"
     CDS             complement(<1..815)
                     /gene="lsrF"
                     /locus_tag="PLR13_03510"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HQC18430.1"
                     /translation="MPDADGIKEAKMFHCDMPQETQGFFLKGSSSLDWGMKNRLSRIF
                     NPKSGRTVMLAIDHGYFQGPTTGLERVDINILPLAPYADTLMLTRGILRSIIPPSLTQ
                     AVVLRVSGGASILKELSNEEIAVDIEDSIRLNVCAMAVQVFIGGEFERQSIINMTKMV
                     DIGNRYGIPTLAVTAVGKDMARDAKYFRLACRICAELGAHYIKTYYVEKDFETVTSSC
                     PVPIVMAGGKKIPELEALTMAYNAVQKGAAGVDMGRNIFQSDAPIAMIQAVRAV"
     gene            complement(1009..1302)
                     /locus_tag="PLR13_03515"
     CDS             complement(1009..1302)
                     /locus_tag="PLR13_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18431.1"
                     /translation="MKMFYVIYAEAADDDIIAAFKKTGFRAYTKISGLMGEGEETEPK
                     LGTHYWPGNNNALMLAVSDDKIKPLCELVRNLKVEHPRAGLRAFTFPLEEECV"
     gene            complement(1344..4451)
                     /locus_tag="PLR13_03520"
     CDS             complement(1344..4451)
                     /locus_tag="PLR13_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQC18432.1"
                     /translation="MWLADTSVKRPVFATMVIMALVVLGIVSYPSIGVDLFPKVDFPI
                     VTITTTLKGASPDVMDVDVADRIEGAVNTINGVKTITSTSTESVSRTIVEFVLERDID
                     QAVQDVREKVSTIRSKLPDDIEEPRIAKVDPDSQPILYMNLTGEKSIRDLSTYVDEVL
                     KEQLQRINEVGDVTFNGLRLRQVRIWLDAAKMRAYEVSPSDVVVALKRENVELPGGRI
                     ETQTKEYTVRIKGEFPSIPDFNDLIVSYYKGAPVKIKDIGRAEDGTEEKRSLARFNGI
                     PAVGIGIQKQSGTNTVAVAARVKKEVERINQTLPPGMKINIAMDQSVFIVRSIDEVQK
                     HLILGSLFAVLAVFLFLKNIRTTLISAVALPVSVISTFALINAFGFTFNNMTMLALSL
                     SVGLLIDDAIIVIENIYRHVEEGMGPVEAAKFATSEIGLAVMATTLAIVAIFLPVAFM
                     KGLIGRFFLQFALTIVFAVLVSLLVSFTLTPMMASIFLKPHNAPPPKGNVITRWSFWK
                     KAGDFLEKNYKRLETFYGRVLKFSLRHRAAMLFATLMVFMFSIFIMIFGLGKEFLPPE
                     DQGNFIIRLEAPIDYSVEQVEKYFRPAEKMVRDIPEIKSVFYVQGYQGYANRGIMIVG
                     LIPKADREKSQEDIKKIVRMNLKQIPGLKVSAEDVSLVGGGIRQVPIQYSIRGQDLNE
                     LQKYARQITTEFAKRPGIVDVDTSLEAGKPEFKVYIDRDKAADLGVDVATIAEAINLL
                     VGGELDIARYKDEKKGKRYDIRVRLNAEDRESSEAMQKIYVRARDGKLVELSNVVRIA
                     EGTGPSVINRADRQRAITIFASLEGKPLGEARNELDEIAGRILPADYVPKYYGMAEVM
                     QESFIFLLFALILGIIMAYMILASQFESFIHPATVLLSMPLSFIGAFGALFLTGKTLN
                     IFSLIGLILLMGLVKKNAILLVDYTNVLRERGMSRRDAILQAGPVRMRPILMTTFAMI
                     FGMLPIAFAVGEGAETRAPMGIAVIGGLLTSLILTLVVVPAAYDIFDDWQEYFKKRRA
                     KKTID"
     gene            complement(4438..5562)
                     /locus_tag="PLR13_03525"
     CDS             complement(4438..5562)
                     /locus_tag="PLR13_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQC18433.1"
                     /translation="MHLKNLVSFIEKKESRRNTVKFIFLISLTAIIAFSLMSCKKEKI
                     VEKEVLTNVRVSTSVKKNIRPYIETTGTLTADEEVTVSSEVDGIVRSVRVEEGSAVGK
                     GTLLAEVNDIDYALDEKRAQAALKQAQANLTNVKAEFQRKEALFKEELITQQQFDDIS
                     TRVALAEADLAKAKATLDTARERLARTKIYSPLRAMVKEKRVSVGDYVRTSMPLFQLI
                     KVDRLKLNFTVGEKELADLKTSQEVDFTVDSFPGRKFKGTVHLLYPHLEERTRTLQAE
                     AVVVNAENLLKPGLFARATIYTGALREVVVAPLTSLLYDDATVSLFVVEGNTARSRTV
                     KTGRKYGEDIEIIEGLNEKEQVVVVGQNNLSDGVKVHVAR"
     gene            complement(5596..6987)
                     /locus_tag="PLR13_03530"
     CDS             complement(5596..6987)
                     /locus_tag="PLR13_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQC18434.1"
                     /translation="MMTVLATALLSAANAVAQEYTLREIYQKALNTSEKMEIARENVY
                     VAQMSKDKALSLLIPRLTAYGTYNRFTEDKYNTANIMIQPEDSAAWGVRADQTFSLSA
                     RELDALRIAGQSISKSEYDLDWAKSDFLLAVASSFYDVLKAQKALDITVANLERLTKY
                     YHLVETRMKVGELTKTALLRAQGELSGAKSDYLKASNALKLARAALTRLTGVEPDFRL
                     KEETVSLSEACDIQGLRKSASDLRTDLKSYDMQTKMAGQQVKYARGAFWPSVGLFAIY
                     NRADQDPAGSTFNDENIYGGISLTFPFFEGGLRMAEFREARAKERQARLAYDDLKKSV
                     DIEVEAACLDLDTLKGSLKFLEDQQIFARDNYNAVMKQFENGLATSLDVMDANTLLLT
                     ADKNVVDALYNYQLASLTVKKSSGTLLQFVRDGDPAVKGSSGALWQLTDVGDLRAKKS
                     GGRLLLFFSIDES"
     gene            complement(7032..8375)
                     /locus_tag="PLR13_03535"
     CDS             complement(7032..8375)
                     /locus_tag="PLR13_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQC18435.1"
                     /translation="MIGKKIRILTIVVMIVFSSRLSFAQEVLSLEASIDLALKNSNIL
                     NIAKEGLNSATAQKREALTGFLPKFSTAYSYSRLNEAPFSRFQGLPPGPMYDLNGMET
                     PVGTKDNYNWIIEARQPLFAGGGILANYQASSIGEEVARLEETARIQDVVRDVKAAYF
                     NILRAQKINEATVQSVEMLKAHRDVAENYFKVGLIPKNDLLFADVELANGRQALVRTQ
                     NAVELAKSRLNTLLKRDISAPVDVMDILAYQPLKQTFEECLETARRLRPEMKISSLKV
                     QQAGKLVRMSQSEYYPALSLVGNYTRFGDEASVHGSDFKDAESWYIMAVANWNFWEWG
                     RTKFRVDASKARENQASESSKELIDQIALEIKNAYLLLQEAQNQISVSETLIKQAEEN
                     FRISEERYKERVATSTEVLDAQTLLTRAKSEYASALGDYNIRLAHLQRAMGNIWP"
     gene            complement(8365..8985)
                     /locus_tag="PLR13_03540"
     CDS             complement(8365..8985)
                     /locus_tag="PLR13_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC18436.1"
                     /translation="MERLERKEREFNMRRAEILEKAEKIFSVKGYHNVTMAEIASASG
                     FSTGSLYQFFEGKENLYSSMISEKLNLMYEETKKATDRADNVLDKIEMLVDAQLRFLE
                     ENADFCRLFIKGESTALSEAMTSMREKIIKDYFKYIGFIENLLKEGIQQKLLKNLPAG
                     DMAQSLVYLIRAVSMEWMLSLKNESPYSKKGFILDIFFNGVKNHDR"
     gene            9322..9597
                     /locus_tag="PLR13_03545"
     CDS             9322..9597
                     /locus_tag="PLR13_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18437.1"
                     /translation="MRKLMQTTLLTAFVVLVTTSFSYAQYSVTGDANFPFFHLGCLIV
                     GGLIIISLKRKYTKLYMSEAIGSFALYALLVSIFTAPVVDALKNLIN"
     gene            complement(9676..10188)
                     /locus_tag="PLR13_03550"
     CDS             complement(9676..10188)
                     /locus_tag="PLR13_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQC18438.1"
                     /translation="MDKKENIERINTNIADRFDKVEKKLSGAGSIAELFEMLLTQIEA
                     EFQVPFIWISVADTIKTGRVIDAFKSSRILKDRLNVIKADQFREIFFSGLQPILVNKN
                     LRPYYRLFPPNQKYFIKSLAMVPFKINGEVAGSWNNGDAIQNRYSPDMETSLLKKLSR
                     QLSARLDELL"
     gene            complement(10198..10965)
                     /gene="xth"
                     /locus_tag="PLR13_03555"
     CDS             complement(10198..10965)
                     /gene="xth"
                     /locus_tag="PLR13_03555"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQC18439.1"
                     /translation="MKLATYNVNSIRSRMHIIVPWLKAHSPDFFCMQETKVADTVFPA
                     AEFEALGYHVVFKGDKQYKGVAIVSKQKPQKVIYGFDSEPSDPDRMIIAAFNDLTIIN
                     TYVPQGQEIESSQFTYKLEWLARFKKYLHKNFRPQNQLIWCGDLNIAPERIDVHDPKR
                     LLGHVCFNPDVWKALEDIKSWGLTDVFRKHHPGIAGQYTFFDYRIRDSVKRGLGWRVD
                     HILATKALAERSKSSAIDLDSRLAKKPSDHLILYAQW"
     gene            11473..12813
                     /locus_tag="PLR13_03560"
     CDS             11473..12813
                     /locus_tag="PLR13_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HQC18440.1"
                     /translation="MQLIESVLQQISDILWGWPLIVLLVGTHIFLTFRLKFIQRYMFK
                     AIKMSISRKREGEGDISQFGALTTALAATIGTGNIVGVATAIAAGGPGAVLWMWLTGV
                     FGIATKYGEAVLSLKYRITNKEGLMAGGPMYVLERGMNARWLGLIFAALTAVAAFGIG
                     NMVQANSIASLVQETFNISPWITGLTMTVLTAVVILGGIKSIARVCEKLVPFMAVFYV
                     FGCLILLGMHYETIPETFKLIFQSAFSGHAVIGGFLGAGMKEAIRYGVARGLFSNESG
                     MGSAPIVAAAAQTKNAIRQALVSSTATFWDTVVVCAITGLVLVNSGEWIHGYKGAALT
                     KAAFSALPVVGPFVLSIGLLTFVFSTILGWSYYGEKAAEYLLGTKAIRPYRYLWVGAV
                     MLGSVLSLPIVWTFADIANGLMAVPNLISLLVLNKVIISETKHFLWEGKLDEPR"
     gene            complement(13196..14512)
                     /locus_tag="PLR13_03565"
     CDS             complement(13196..14512)
                     /locus_tag="PLR13_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18441.1"
                     /translation="MMKTAILLYHFFKSLIEIHLSARLPYGLQRKLCERRLIRSIRHA
                     YDHVPFYRQKYDAAGVDITSIRSADDIKRLPFVTKEEIRQNFPDGIVARGVNREACRY
                     SATTGSTGRSLPFIFTQATYAFYVALGVRMYTMIGYRPWHKMAYIKYTDLNYTRLGPF
                     FRAVHIKSTIPVEEQIAQLRKAGADLIIGYASLVYEIARNATPDDLKDIRPKFIGINS
                     ELSTEAQRTFISETFGCPVYDEYSTEETWMIASQCRCNNYHIFTDNVWVEFIDAKGQE
                     VAPGDMGEIVLTTTRSPAMPFIRYRIGDMGRFSNAECPCGMGFPLLQSFEGRADDCFI
                     LPSGKFVSSLKLLNTFTMYIKKYLHLLEEFKVTQVHSDLIVIKIVKGNQYDSAHLEEL
                     LADLRRLLDDSVSIKTEFVDVIEKTDGIKRKAIESLVSQKPTGSPS"
     gene            complement(14525..17683)
                     /locus_tag="PLR13_03570"
     CDS             complement(14525..17683)
                     /locus_tag="PLR13_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQC18442.1"
                     /translation="MWISDTSIKRPVLATMFILALVVLGLVSYPSIGVDLFPKVEFPL
                     VNITTGLAGASPEIMDVDVTDKIEEAVSSINGVKTIHSLSIDGVSSVIVEFELERDID
                     LAVQDVREQISAVRSQLPDDIIEPVIQKVNIDASPVLWLALTGDKSQRELSTYVDESL
                     KEKLQKIQGVGALSLAGMQKRQIRVWLDSNKLAAYGVSPGDVAAALKRENIELPGGRI
                     ESLTKEFSIKVKGSLKDVSAFNDLIIASYAGVPVRLRDIGRVEDGTQERRSYARFNGI
                     SAVGIGIQKQTGTNTVQVVDRVKEEVERIKKSLPPGMDIRIAFDQSVFIKQSMDQVQE
                     HLILGSILAIIAVFIFLRNFRTTLISAVALPVSIISTFAIMRIFDFTFNNLTMLALTL
                     SVGILIDDAIIVIENIYRHIEDGMSPREAASFATDEIGPAVMATTLAIVVIFLPVAFM
                     KGIIGRFFMQFSLTVVFSILVSLLVSLTLTPMLSSLLLHPRKKESLDSERPGTVMARI
                     GQTLERGYKKIEEAYRPILTFSLDHRRAVLMSALILFIFSLWITRFIGKEFKPGEDQS
                     QFMIRMESPIDYSVEHAGAMFSQAEKIVRNRTEVTSVFYGQGAGQGRLSQINQAFIQT
                     SLKPRSDRDKSQSEIMAELRKEFTAIPGLKTTIEEVAILGGGIRNVPVQYSISGGSID
                     ELRKYAKQIVGELSKIKGLVDIDTSIEAGKPELSVYIDREKATNMGVSVAAVTEAVNL
                     LMSGEVEVTKFKDTAKGRRYDVRMRLDAKDRMSPSDIGRLYVRAVNGELVRLSDIVTV
                     EEGGGPSSISRKDRQRTIWIFANLQDKPLGEAMQEIDAISARILPAGYVAGYSGEAEE
                     MGKSFKYLMFALLLGILLAYMVLASQFESLLHPVTILLSMPLSFIGAFAALLIFGKSL
                     SIVSFIGLVLLMGLVKKNAILLVDFTNTLRERGLSRREAILQAGPVRLRPILMTTFAM
                     VFGMLPVALGIGEGADLRAPMGITLIGGLLTSLFLTLAVVPAAYDIFDDWKENMIRRL
                     GSRGASPWIRPFGKNKKENNNN"
     gene            complement(17693..18889)
                     /locus_tag="PLR13_03575"
     CDS             complement(17693..18889)
                     /locus_tag="PLR13_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18443.1"
                     /translation="MTYSRQLSSTERAYVVYNECCPPFLNQLFWEGTGYFDLPKWREA
                     VKIASEANPGSRVVVKGITAFARLVDTGKTPPVIEVDGTHWDGLGPEGAPAKLSETLN
                     PFEGPTSEVVLIHGNPLRVVFRTMHATMDGRGTVTWVEDIFRGLRGEAPLGSDSRITE
                     FEIAATFQKQGRKGPVPEYIAPTGMPQGDQPGVIWRRRRYPGRYHNVLGQVATLLAGK
                     AWQYQDGKVRLAIPVDMRARVEGLRSTGNLTNLIYVEINPSTTPADVSQDIKRQLEAR
                     HDGMIYWGDRPIRFLPLWLLKKLIKSNIATKNRTGRYPNSGVLTNMGRIPVFSGGGFE
                     GKYTWGIPPGQEYSPIFIGMCNSDDDMTMIYSMPNVLAGGGRLETLMDHIQQGLVASK
                     ADRKKG"
     gene            complement(18886..20067)
                     /locus_tag="PLR13_03580"
     CDS             complement(18886..20067)
                     /locus_tag="PLR13_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18444.1"
                     /translation="MTFNDRAFVVLNQISPPTVNQFIFDGEGTLDPEKWRRAADAASE
                     ANPGTRLVLRGHLSWSRWVDSGINMRVREVDGRNWDGRGPENEPLLEESLPYRGNPTC
                     EIVLIQGPTPRVAFRSHHAVCDGRGTLFWAEDVFRTLRGEAVVGSTSNITDVELCKSF
                     QNQYRTPFPAEHIAPTGRAKGNDTGVTWRRCSLKGKMPHLLAQCARLAAEEAWRHGEG
                     VVRFGIPVDLRHRRPELRSTANLSFALYTEVKKETSPDDIARDIALQISRCDECRLTK
                     GDERLEYMPLWMMRYKAKNIIRERHRQGLYSLSGILSNLGRINLNRFCGGGFTARAFW
                     IIPPAAEYYPFFMVMTGYEGGSDLTISMPKVLASEGRLENTLKRIVHGLEGTNQREDT
                     I"
     gene            complement(20088..20897)
                     /locus_tag="PLR13_03585"
     CDS             complement(20088..20897)
                     /locus_tag="PLR13_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18445.1"
                     /translation="MSRALHLAVVMLVTAFSIQTAVAQEHFLHALGHFETLSTTQGIL
                     IAAPHGTYDIRTDVLAKDVARTLGAGYVIAGGFASPSPRINVNRPSEGAGHTCEKERR
                     TERARDVYDQYARLIDKIRDGKPLRLYVEIHGHTSIPFLNRLEIATTGITTEEARRLK
                     DRFPAFLAQAKAAYPAYPDLELRIEPLDKIYFGARCNKTTGYLSTRNMERALHMEIPR
                     PARTPEALKATAALVSSIVRATMTISESASGPGPKRATSDADKKSSGGPAR"
     gene            complement(21023..21964)
                     /locus_tag="PLR13_03590"
     CDS             complement(21023..21964)
                     /locus_tag="PLR13_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQC18446.1"
                     /translation="MNTKLWKQLLPLAVSGAILLYLFRHIDIRMCFNAMLTANVSLYI
                     PSLIALILITFLLDTQNLAETLKHFHYPLSWKNAFTLRGVTYLIMTIDYSVGMGVLIY
                     YLKNHLGIPVMRSTGLMTFFNGITQKALVYMAIIGLVILSPASGLLRNLLIFFIGFAL
                     LDILFIIVLKKLPSRGLALKIKSMNLLKVFHEAPWRTYGVLIFWRILYYACFVAFFYI
                     AVRAFNMQIPLVALIAYVPIILLVISLPIAPGGFGTAQATMLLLFKDYGTPLNIMAFG
                     LIYTTSILVLRYLIGLLYAKHIKGLPAGEDSTSEQRN"
     gene            complement(22143..23516)
                     /locus_tag="PLR13_03595"
     CDS             complement(22143..23516)
                     /locus_tag="PLR13_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18447.1"
                     /translation="MIKLQKGYLKISCVLMCLLALIFATRTWACDVAVVSGKVTTDGK
                     PLIWKNFDNSSSDRQQVAYFPAKKSGPGGYLMVYRFENGMKLLNNGSAITPSGGVNES
                     GFAIAGTSVYQDYNLMAEPVNINTVLMEEALATCTTLAEFESLLKRWPYYHWGTVISA
                     NFVVIDAKGGAALYECFTGHLNTGLNLMQFRKNDANTGKITNQYGWTLKRAQSNFIGF
                     HIMTNYNYYIPWNVGQDRKYRAEALLTQLATEKRINYRTVMREVAKDVVGKQVQTDLS
                     SETNYSTTYCLSRNATRSSIVVRGVASGDNPRLATMWVNLGEPSVGVFVPFFAAARGT
                     SELARVDTYANGAWRDYDDSCLLNQAISARETYGSLIYSSNEGDVIWGMYDNYINKVE
                     LAKVQQWTFQIEDTIIDNAESFLSSLQSNPAQITAFNLKDFADYCADYAYENFETGSA
                     DAFPWQY"
     gene            complement(23602..24555)
                     /locus_tag="PLR13_03600"
     CDS             complement(23602..24555)
                     /locus_tag="PLR13_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18448.1"
                     /translation="MKSMSVSSASEQRGHQSDSTDPLPVNAQDFFIQSHKGFNYYLID
                     LNTAEWNRLGDIIRGCEAVENDCWKAQEEFSTYLRKCDVLSYVEKNGRIVAFDAVSIL
                     NSGPHCVYNNEETMVLKAFRGRDLAQRLVIMTVEWHLTKAASIKNKDYFIFTSISANP
                     AVVNRYFNKSWLRVLFDSSFSPSPALIALKEEYCRKYGIGLVDQSYPFCLKNMFPGSN
                     GFDPNDPKYQFSPHVRANMPADFEHMDRGDAFAFMLMIPMKIVRLVTFVLMIKCFGKE
                     YFSSRGVGLFSSRRPLIPSFTDRRVPGLSGEPFSVKTADHG"
     gene            25532..27274
                     /locus_tag="PLR13_03605"
     CDS             25532..27274
                     /locus_tag="PLR13_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQC18449.1"
                     /translation="MNKRKKLGEILIEDSIVSEGQLMSALALQKKSGKMIGQTLIELG
                     YITEAQLVEAFRKKFSISYIDCDGVVVPEKFLKLVSMTMAVKKKILPTDVSGNTLFLA
                     MVNPLDYSTIDEVKFLTGKNITPLLTTESSLMKAIEKNYGYCSIEKIWKPLNDIHLDG
                     EMQFNLDKSIEFHKMEEEDESALRADDLYKIGEEPPIVKLVTLILVNAVKSRATDIHI
                     EPHGKYVSVRYRIDGDLSTVLKISKTYQEAMTSRIKIISTMNITIRRMPQDGRSTVKY
                     EGRMIDLRVSTIPSAHGETIVIRILDSVKGLIPLSKLGMPDNIIRDLLEMLNKPQGML
                     FVTGPTGSGKTTTLYSLLTQLSLESESVVTIEDPIEYELENIKQVPVNEKVGFTFAAA
                     LRSILRQDPDVIMVGEVRDSETAQISTKAALTGHLVLSSVHTNDTIATISRLRDLGIE
                     PYLISSALNAILAQRLVKKICENCRVEFEIPQYLFENYPYLKKLKIGISYKGKGCEAC
                     GFTGYKGRIGIFEFLRVDGNLRRTIPLYHSDQDLCIVARENGMKTLIEDACEKVEHGL
                     TTINEVLTKIHYYE"
     gene            complement(27305..27745)
                     /locus_tag="PLR13_03610"
     CDS             complement(27305..27745)
                     /locus_tag="PLR13_03610"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQC18450.1"
                     /translation="MRSDIQKIIQENRMCVMATVGRDAPHCSLMSYASGRDGREIYMA
                     TFKDTKKYHNLIANPNVSLLIDTRVVDAEGTPRALTVTGIVEAVDNDKSIEEIRETLL
                     RKHPDLRDFFNHPDTRMIVVRPTTLQLLDNVTDAYFEELRQPKR"
     gene            27877..29001
                     /locus_tag="PLR13_03615"
     CDS             27877..29001
                     /locus_tag="PLR13_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQC18451.1"
                     /translation="MKNKKPFLTEVRSQDVLSVSIPDTRSFAGAQRKSFRVWWIAFRY
                     HFVPASFLPAILGSVGAWVQTGLMNRWFFFLTVLGVTLNHIALNMTDDYFDYHAAVDQ
                     GGERRENPYTGGSGTLTGGLIKHGHMRLMFTLFYLITIGIGLYLCAAQTWWIIVFGLF
                     GVGCSYFYTAPPIRYGYRGLGEFSQLINFSFTIGMGAYVVQTVSFSWEIFWILLPLGF
                     MMFAMIVINEIPDRFDDAAAGKNTLVVRFGLKAAARFYGVAMMLAYAVILLAPPLNMA
                     GYWIYLGLLTLPWSVRAFLIMKAYGNEPDKLAPANLLTIRVHNFTGILLIIACLIQGL
                     QDRQVFQPVVFAAVILSAFYLPAAISIFFKKMPDGRRTEA"
     gene            29082..29801
                     /locus_tag="PLR13_03620"
     CDS             29082..29801
                     /locus_tag="PLR13_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HQC18452.1"
                     /translation="MKMDKETLSKHDGREGHKAYVAVEGRVYDVTGNRLWKNGVHMNR
                     HQAGTDLTEAIAASPHGKDVLSKLQDMGQMSPEKVAKPPFIPLWMHHWLEAYPFFKRH
                     PHPMVVHFPMAIFIIAPLFLAWYYLINPVPGLPAAVFYLYILGTLSLPAAIATGLLAW
                     LVNYSGQPNPLIIRKTIFSLLLLLCDGIVMAALIISPAILQDPSGSEWMIPVLIFLGL
                     PLASIIGEHGGRLVFPVLKNK"
     gene            30088..30546
                     /locus_tag="PLR13_03625"
     CDS             30088..30546
                     /locus_tag="PLR13_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQC18453.1"
                     /translation="MPSKKLKEFLNSQSVKYVIIAHSTAYTAQEIAQSAHIPGTELAK
                     TVIVYIDGKMAMAVLPASYSIDFDDLRKETGAKKVELASEAQFKDMFPDCEIGFVPPF
                     GNLYGMDVYVDEHLTRNKEIAFNAGGHLELYRMSYRDFEELVKPKVLKFT"
     gene            30597..31343
                     /locus_tag="PLR13_03630"
     CDS             30597..31343
                     /locus_tag="PLR13_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQC18454.1"
                     /translation="MRESVMELNIIRKAMAHKTISLHVLLTVFLVTNYSAITGAAETG
                     KKSLLPDRSVITLPAPVYQGRMSVEEALRKRESVRQFSQSPLTEQELAQLLWAAQGKT
                     RDWAARTAPSAGALFPLEVYVVIREGVFRYSSGDHRILRVVKTDVRRQLAAAALGQDI
                     IRQAPAVFVIAGVYKRTAEKYSNRAKRYVEIEAGHAGQNLLLQAVSLGLGAVPVGAFQ
                     DAHVKQALHLPVNHEPLYLIPVGRKRFVPP"
     gene            31505..31795
                     /locus_tag="PLR13_03635"
     CDS             31505..31795
                     /locus_tag="PLR13_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18455.1"
                     /translation="MGDFDKRKYIRYPAEPNELVTVYYMDEKTTKTGQRVGLGENESF
                     KGCCVVFVGDVDFKQGQEVFCECGTLPKTKAQIVWVKKLENQITKVGISFRE"
     gene            complement(32085..32690)
                     /locus_tag="PLR13_03640"
     CDS             complement(32085..32690)
                     /locus_tag="PLR13_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC18456.1"
                     /translation="MSGKTIHEKAEYRSAIRSRKLIKQAYVSLIKEKDIDKITVMDIV
                     TTADINRGTFYAHYSNVNAVGEQIGNEIISALLEFLDEFKNSRLIENPLPFLMNIARF
                     LEKDLEFYRILINSQRSIIFLNKLKGIFLDKIVSDEKKMSAIKNKEQFMVCVNLYASG
                     FVGLYQDWFNNRIKMSLDDLTLHFSEIIKNGFHAFLPKKNR"
     gene            complement(33543..34490)
                     /locus_tag="PLR13_03645"
     CDS             complement(33543..34490)
                     /locus_tag="PLR13_03645"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HQC18457.1"
                     /translation="MDKKISGQDAEKRMTPLQRDVAFGNATEPPFQNEYWNNHQEGIY
                     VDIVSGVPLFSSKDKFDSKTGWPSFTKPIKDNAVWRKTDFSHSMLRTEVRSTQADIHL
                     GHVFDDGPAPTGRRYCINSASLKFIPREKMETEGYGEYLSLFAAPSGKSTETAIFAAG
                     CFWGVEEYFSRVKGVVNSESGYTGGTVKAPSYEQVCSGKTGHAEAVRVTYDPTIVTYE
                     RLLQHFWELHDPTSLNRQGNDVGTQYRSAIFYTNPQQEKAARASAEKLARSGRYTRKI
                     VTEIQPAKDFYPAEDYHQDYLKKNPRGYCHIDLKKAKKD"
     gene            complement(34683..>35828)
                     /locus_tag="PLR13_03650"
     CDS             complement(34683..>35828)
                     /locus_tag="PLR13_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18458.1"
                     /translation="QKRWSYIPINRGRGCNWNCEFCVVNKQYGKYKAASIDTALRQLI
                     RYADMGYTKFFFTDDNFAQHPDEAIALCKAIGHYKRNFLKKIDLVVQVRSEVAENDAL
                     IEAMHFAGVSMLAIGYESPINEELRAMRKGVTVEKLIARSRKLSNVFYLHGMFIFGYP
                     LNDDAQGPSSFTVKQRAKIYKDFFKKSGIDTIQVLNAVPLPGSDLRTRLEREGRLLPL
                     KIVGWDKYDGLFLCYDPRPDSMDAFELQGMPGLLMKKKYLGGFIKRNLNYGNWLNWTY
                     IATIGFPIQFVEFYIRRFIRNLAERQRAGKRPDGAWSPRTIFAVPLISAWKDIKRIWR
                     NLFVRTYAGAIVNRWLKIYRKSRYGDKLRKFFLDSKITPKSRHRLNT"
     assembly_gap    35829..35928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35929..36238)
                     /locus_tag="PLR13_03655"
     CDS             complement(<35929..36238)
                     /locus_tag="PLR13_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18459.1"
                     /translation="MERDLTVIGIIPQYPKHSQQNIYARIKMPPVGIISVMSQIVRHP
                     RIRNIYAIDENNYRGPEDLNGLPDHFFLQQQQPAHLALLYGGMSNSIPRMFAIARQYK
                     S"
     gene            complement(36509..36709)
                     /locus_tag="PLR13_03660"
     CDS             complement(36509..36709)
                     /locus_tag="PLR13_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQC18460.1"
                     /translation="MSEGKVKWFNESKGFGFIEKEEGGDVFVHFSSIQGEGFKTLNEG
                     QRVSFDVAQGKKGQEATNVRAI"
     gene            complement(36993..37907)
                     /locus_tag="PLR13_03665"
     CDS             complement(36993..37907)
                     /locus_tag="PLR13_03665"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC18461.1"
                     /translation="MQCTPPDTAAACLLAAEADLFLKEYQQHYSAGETKPQQIGMPFF
                     LHAPGSDTGVLLIHGLMAAPEEVREWAEFLHQKGLTVYAPRLAGHGTSCKDLSTRRYE
                     DWLDSAERGHAILKKYCDKILVAGFSTGAGLALQSVILKPHDFEAVIAVSAPLKFKSF
                     SSRFAEVLNAFNRFCHSRGVNTLARDYIKNDADNPHINYLLCPVSAFVQVKKLMKKVN
                     RSLPDIRIPALVIQAKNDPKVAPQSGPEIFRRLGTNKKRFAWIDHDLHGIVRGNISGM
                     VFKEVELFLAEQNLIHIRPEMSNRLPGA"
     gene            complement(37919..38569)
                     /locus_tag="PLR13_03670"
     CDS             complement(37919..38569)
                     /locus_tag="PLR13_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQC18462.1"
                     /translation="MNVKTQELFNFYSHLAGMIAAVIGTVYLAIEASFSASGLVTALI
                     YGISVVFLFSASSLYHAFKKKENELSFWRRMDRLAIFFMIAGTFTPVCYFCLEGNQRW
                     AMIAFQWSLVGVGVISQIFFPRAPRTLYAMLYLFMGWSGLFIIKQVLASMSISQTVLL
                     FTGAGAFTAGGIIYAIKKPKLIPGIFSFHELFHILVLIGGALHYAMIYGVYAQLGA"
     gene            38861..39316
                     /locus_tag="PLR13_03675"
     CDS             38861..39316
                     /locus_tag="PLR13_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HQC18463.1"
                     /translation="MTKQETINSAVSFFNEGYACSQSVLLAFAGRFGLDEKQAKRIAS
                     TFGGGMGRLREKCGAVTGGFMVLGLAYGNADPNDMETKLTAYRKVRGLNRKIEDLHGS
                     SCCSELLKKHATTADVTERKHHQIICQKIVGGVAGILYDMLEEEPPRAR"
     gene            complement(39417..40238)
                     /locus_tag="PLR13_03680"
     CDS             complement(39417..40238)
                     /locus_tag="PLR13_03680"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQC18464.1"
                     /translation="MNEKTGNASSPGFYGRPTRQDGQLSAVPAAHLRLSAHFRMVIFI
                     ATLCLSFCALNCRAGVEQNDDVLRKKMVEEQLKARGIKNPQVLRAMSKVERHQFVPEA
                     LRKMAYADSALPIGEGQTISQPYIVAWMTEVINPQADMKVLEIGTGSGYQAAILAELC
                     HSVYTIEIIETLARRAEKILGKHYQNVHVRTGDGYQGWPEAAPFDAILVTCAPTKVPA
                     PLAKQLKEGGRMVIPVGNVKAQELYVLVKRNGRLEQQAIIPVLFVPMLDKEGEKY"
     gene            40478..41086
                     /locus_tag="PLR13_03685"
     CDS             40478..41086
                     /locus_tag="PLR13_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18465.1"
                     /translation="MKGYFKIISMFVGMCVLLCASWADAGVYYKTVQQPPAASEKIKA
                     GMDFSVLPIDYSAINPKDLGYTNNAEWDADKSAVPKAFADAFPVLLKEANITNKKISV
                     VSGGDKITQGVIAQVAVTKINLNWNAWTNRPDEYVCKITFTDAADGQKLFSGEVNVST
                     FSGNPFTSAWGSFSGRMQSAAYNMAWVLTKIMTQGRIEPAEH"
     gene            41236..42918
                     /locus_tag="PLR13_03690"
     CDS             41236..42918
                     /locus_tag="PLR13_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQC18466.1"
                     /translation="MTDETASTPTDFIRDIINDDLKTNKNNGKVATRFPPEPNGYIHI
                     GHAKSVCLNFGIARDYKGTCNLRLDDTDPAGESMEYVQSIINDIRWLGFDWEDRLFYA
                     SDYFEKLYQFAVELIKSGNAYVCSLNPDEVREQRGTFTEPGKESPYRNRSVEENLDLF
                     QRMRAGEFADGAHTLRAKIDMASPNIVMRDPVIYRIKREPHYRTGTQWVIYPMYDFAH
                     CLSDALEGIVYSICTLEFENNRPLYDWIVEKLITGLRPRQIEFARLNLSYTVLSKRRL
                     IELVEKKYVRGWDDPRMPTVSGMRRRGYTAEAIRNFCAGIGVAKNDNLIDVALLEHCV
                     REDLNEHAPRAMCILRPLRVVIDNYPDNQVEQIACANHPQKPDMGTRNVPFSKVLYIE
                     RDDFMEDPPKKYHRLGPGREVRLRNAYVIKFESMMKDEKTGDVTELHCSYDPATLNGP
                     PADGRKVPGVIHWVSAGHAVPAEVRLYDRLFSIENPGSEEDFLKYLNPDSLETLTTCR
                     AEQNLKNAAAGSRFQFERLGYFCVDAKDSTSERLVFNRVVPLRDSWAKIAGK"
     gene            42997..44217
                     /locus_tag="PLR13_03695"
     CDS             42997..44217
                     /locus_tag="PLR13_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQC18467.1"
                     /translation="MKTSGKTALLFTLYLSQGLPFGFQATALPVLLRDKGISLTTIGF
                     AGALALPWFFKPLWAPLIDRFWSARIGRRRSWIIPLQTMMVLTMFSSVFIAASGSIPL
                     LLLCIFLMNLCAATQDIAVDGLAVDILQRDELGPGNAAQVAGYKAGMVLGGGILVWLS
                     ARSGWESLFLGMAALAVLPLIVILCHREVEPDGSIVSKPPKFSDFISAMRNALQSPKA
                     GYFLLFIATYKIGESMIDMMFKPFLVDNGFTVQQIGLWVGTWGMASSLIGSVAGGWLA
                     ARISIVRALFLAASLRLIPLIGELWLSTFVPTAAQVVSVTVAEHFFGGMLTTALFAFM
                     MSRVDKKIGATHYTVLAAIEVFGKFPGTVFSGCLAENIGYTGLFSFGVVLSLLVLLLY
                     FPLRESTSPPKMNI"
     gene            44509..45156
                     /locus_tag="PLR13_03700"
     CDS             44509..45156
                     /locus_tag="PLR13_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQC18468.1"
                     /translation="MKKILSVIVGFMMLMMIPGISIGSDASPAKKAKAAKFKAAAVNK
                     PYVSVQGGVAFLTDSDVDAGIRSTAMDFDPGYALGVAAGYKMGKYRVEGEIGYQKNEV
                     DEALSGRRTSGDMKAYSFLVNGYYDIATKTRFTPYLTAGIGVAKVDGDFNRIAHVDDT
                     AFAYQVGAGVAYAVNRALAVDLKYRYMDTVDDFGFGNTNPEFSTHNVFLGLRYCF"
     gene            45389..47509
                     /gene="aspS"
                     /locus_tag="PLR13_03705"
     CDS             45389..47509
                     /gene="aspS"
                     /locus_tag="PLR13_03705"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQC18469.1"
                     /translation="MNYSQRVFCGELSQEDVGRTVQLCGWVDAYRDHGGLLFIHLRDR
                     SGIVQIVFSPDVTPANVCQTAATLRPEYCITVTGMARKRMEGTENPNIETGKLEVFVD
                     GMTILSESDPLPFVITDKAMVAGGETAGAENVSEDLRMQYRYLDIRRPAMQNNLLLRH
                     RIFQGVRQFLDSRGFVEVETPVLTASTPEGARDYLVPSRVHPQNFYALPQSPQLFKQL
                     LMIGGLERYFQLARCFRDEDLRPNRQPEFTQLDIEASFIDEEFLYELIEELTVKMFAV
                     GGIELKRPFARMSYAEAMDTTGSDRPDLRFGLRFVDVTDAFKSTNYSIFRQILQRGGC
                     IKGINIKGQSEKLSKNILQNEYAKDIAPSFGAKGMTWMRAENGKLESNIVQFFSEAEL
                     DELKRRFDVHDGDVLIMVADPSYSIVTSALGQLRLHLADRLGLIPENSFYPVWITEFP
                     LFEPTDEGGVTSSHHPFTAPDRTDFDPKNIEELLSLKSRAYDLVMNGEELGGGSIRIN
                     NRDLQRKIFAALGLTEEDMKEKFGFFLRAFDFGAPPHGGLALGMDRTVSMILQTASIR
                     EVIAFPKNRSAACPLTGAPSAVKREQLAELGLLNLGEKDVLPGEAHKENLLDRVSWVS
                     RLGIAEAERPVMEEILAQSETLAAQVHDLAGAEEPIRSVAPAAGKTRPGAEAIRSPLA
                     ETGQLLKSAPAVKGNYFKVANIIE"
     gene            47524..48720
                     /locus_tag="PLR13_03710"
     CDS             47524..48720
                     /locus_tag="PLR13_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQC18470.1"
                     /translation="MDSIFFYQNPEQSAGSGKGALQKLKVTVQPNIFVKGWPTEAGSA
                     ALAGFKALEDAAVVERLSGAGAVLIGSTAMSEFGFGLKGSQAGCALGKKSADVELMLD
                     MAGEARLAAAREGVCGFKPSYGLISRIGLVGLIPSMETCGILARDIKSVRDILETIAG
                     PDERDFSLPDEKMPDFSAQDIEPAKTTLGIIPEVVNGLPSDQREAFAKICDALKKDGF
                     QIKELSFPDFSHFLLVHQIVGSVEASSCAGRYDSVRYGQRVPGAKNWNEMYLQSRGAA
                     FGTLLKSYLFQGAFFQFEKYDAYDDACRLRARLVKEMQTVAGQADALILPTVNRAASD
                     KTGSLADLYAQFAYTAFANVTGRPALHIPGANGAGVQLSAKRLDDGRLLSLGQYLLSH
                     RQGGKS"
     gene            48720..>49171
                     /locus_tag="PLR13_03715"
     CDS             48720..>49171
                     /locus_tag="PLR13_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HQC18471.1"
                     /translation="MEFEAVIGLEIHVELNCATKLFCDCPNNPGDEPNANTCPTCLWF
                     PGAVPRLSRAALEKAALLCLGLNAELQPESAFDQKVYYYPDLPKGYQLSQAHLPLSRG
                     GWIDIVDEDGSTKRLRIHHIHMEEDVAKLVHEMEGRLPISLVDFNRAGA"
BASE COUNT        11731 a        12299 c        12587 g        12454 t
ORIGIN      
        1 acggcgcgca ccgcctggat catggcaatc ggcgcatccg actggaagat attgcgtccc
       61 atgtccacac cggcggctcc tttttgcacg gcgttgtaag ccatggtcag ggcttccagt
      121 tccggaatct ttttgcctcc ggccatgaca atcggcaccg ggcaggaaga cgttacggtt
      181 tcaaaatctt tttccacgta ataggttttg atatagtgcg caccgagttc cgcgcagatc
      241 cggcaggcca gccggaagta tttggcgtcg cgggccatat ccttgccgac ggccgtcact
      301 gcgagcgtcg gaatgccgta ccggttgccg atatcgacca tcttggtcat gttgatgatg
      361 gactgccttt cgaattcgcc gccgatgaaa acctgcactg ccatagcgca gacattcaga
      421 cggatggaat cttcgatatc cacagcgatt tcttcattag agagctcctt gagaatgctg
      481 gcgccgccgg aaacccgcag aaccacggcc tgggttaacg acggcgggat aatactgcgc
      541 aggatgcccc gggtcagcat gagcgtgtcc gcgtaaggcg ccaggggaag aatgttgata
      601 tccacccgtt ccagtccggt ggtcggtccc tggaaataac cgtggtcaat ggccagcatg
      661 actgtgcggc ctgatttcgg attgaatatc cgggaaagtc tgtttttcat tccccaatcc
      721 agcgagctcg atcccttgag aaaaaaaccc tgtgtttcct gaggcatgtc gcaatgaaac
      781 attttggctt ctttgattcc gtccgcatcc ggcatatttg ctttctcctt tcccgtgatg
      841 aatcgttgtc cagaaattcg tgaagacgtt tttgttagac gcaatcttgc acattttttt
      901 taattcgtca atttgacact ggcggcagac aatccatgtc cggcagtgag tcggaggatg
      961 cttctgaata aaacggcgat tgtccgccct gggtgggcac agcaggactt aaacgcactc
     1021 ctcctccagt ggaaaagtaa acgccctcaa accggcgcgc ggatgctcaa ccttcaggtt
     1081 tctgacgagt tcacaaagcg gttttatttt gtcgtcactg acggccagca tcagggcgtt
     1141 attattcccg ggccagtaat gcgtgcccag tttcggttcg gtttcttctc cttcgcccat
     1201 caatccactg atcttggtgt atgccctgaa tcctgttttt ttaaaggcgg caataatatc
     1261 gtcatccgcc gcttccgcat aaatcacgta gaacattttc atgatcgtca cctcttggtg
     1321 gaaaaggtat ttggaaacat tgatcaatca atcgtttttt ttgctcgtcg tttcttaaaa
     1381 tactcctgcc agtcatcgaa tatgtcgtag gcagcgggca cgacaaccag cgtcagaatc
     1441 agagacgtca aaaggccgcc gataaccgcg atgcccatcg gagcgcgggt ttccgcgcct
     1501 tccccaacag caaacgcgat cggcagcatg ccgaaaatca tggcaaaggt ggtcatgaga
     1561 atcggccgca ttcgtaccgg tccggcctgc aagatggcgt cgcggcgcga catgccccgt
     1621 tcccgcagaa cattggtgta atccaccagc aaaatggcat ttttcttcac cagtcccatc
     1681 agaagaatca gaccgatgag gctgaagata ttgagcgttt ttcccgtgag aaacaaagcg
     1741 ccgaatgctc cgatgaatga cagcggcatg gaaagcaaaa ccgttgccgg atgaatgaag
     1801 ctttcaaatt gtgaagccag aatcatgtac gccatgataa tacccagaat cagggcaaag
     1861 agcaggaaga tgaaagattc ctgcataacc tcggccatgc cgtaatactt cggcacgtaa
     1921 tccgccggca gaatccgtcc ggcgatttca tccagctcgt ttcgggcttc gcccagtggt
     1981 tttccttcca gagaggcaaa aatggtgatt gcccgctgac ggtccgcgcg gttaatgacg
     2041 ctggggcccg tgccttcggc gattctcacc acattgctca gttccaccag ctttccgtcg
     2101 cgggcgcgca cataaatctt ctgcatggct tcggaacttt cccgatcctc ggcgttgagc
     2161 cttacccgga tatcatagcg tttgcccttc ttctcgtcct tatacctggc gatgtccagt
     2221 tctccgccca ccagcaaatt gatggcttca gcgatcgtgg ccacatccac acccagatcc
     2281 gccgccttgt cgcggtcgat ataaaccttg aattccggtt tccccgcttc cagggaggta
     2341 tccacgtcca caattcccgg tcgtttggcg aattccgtgg tgatctggcg ggcatatttc
     2401 tgcaattcat ttaaatcctg tccgcggatg ctgtactgaa tcggcacctg tctgatgccg
     2461 ccgccgacca gcgaaacatc ttccgccgac accttcagtc cgggaatttg tttgaggttc
     2521 atgcggacaa tttttttgat atcttcctgg cttttttcac ggtccgcctt gggaattaac
     2581 ccgacgatca taatgccgcg gttggcgtat ccctgatagc cctgaacgta gaagaccgat
     2641 ttaatctccg gaatgtcgcg gaccattttt tcagccggcc tgaagtactt ttccacctgt
     2701 tccacggaat aatcgatggg cgcctccagg cgaatgataa aattgccctg gtcttcgggc
     2761 ggcagaaatt ccttgccgag gccgaatatc ataatgaaaa tgctgaacat gaaaaccatc
     2821 agcgttgcaa aaagcattgc cgccctgtgc cggagcgaaa atttcagcac gcggccgtag
     2881 aacgtttcca gtcgcttata atttttttcc agaaaatcgc cggctttttt ccaaaaggac
     2941 cagcgggtga tgacgttgcc tttcgggggc ggggcattgt gcggtttgag aaaaatcgag
     3001 gccatcatcg gcgtcagggt aaaggaaacg agcagggata ccagaacggc aaagacgata
     3061 gtcagggcga attgcaggaa aaaccggccg atcaggcctt tcatgaaggc caccggcagg
     3121 aaaatggcga caatcgccag ggtcgtggcc atgacggcca gaccgatttc cgatgtggcg
     3181 aatttggctg cttccacggg acccattcct tcttccacat gccggtaaat attttcgatg
     3241 acaataatgg cgtcatcgat cagaagaccc actgaaagcg acagcgccag catcgtcata
     3301 ttgttgaagg taaagccgaa tgcgttgatt aacgcaaaag tggaaatcac ggagaccggc
     3361 agggcaacgg cgctaatcag ggttgtgcgt atgttcttga gaaaaagaaa aacggccaga
     3421 acggcaaaga gactgccgag aattaaatgc ttctgcactt catcgatgga tcggacgata
     3481 aaaacggatt gatccatggc aatgttgatc ttcatgccgg gcggcaatgt ttgattgatc
     3541 ctttccactt ctttcttgac ccgcgcagcg acggccaccg tattggttcc cgattgtttc
     3601 tggattccga ttcccacggc cgggatgccg ttaaaacgcg ccagcgatct tttttcctcc
     3661 gtaccgtctt ccgctctgcc gatatctttt attttcaccg gcgcgccttt gtaataactg
     3721 acgatcagat cgttaaagtc cggaatgctg ggaaactcgc ctttgatccg gacggtatat
     3781 tccttggtct gtgtctcgat ccggccgccc ggcagttcga cattttcacg tttcagggcg
     3841 acaacgacgt cggagggcga aacttcgtac gcccgcattt ttgcggcatc cagccagatg
     3901 cgcacctgtc tgagccgcaa cccgttaaaa gtcacgtcac cgacctcatt gatccgctga
     3961 agctgttcct tcaatacttc atcgacgtag gtggaaagat cgcgtatcga cttctcgccc
     4021 gtcaggttca tgtataaaat cggctgggag tcgggatcga ctttggcaat gcgcggttct
     4081 tcgatgtcgt ccggtaattt gctgcggatg gtggatactt tttcacggac gtcctggact
     4141 gcctgatcga tatctctttc cagcacaaat tccacgatgg tgcgcgaaac gctttccgtg
     4201 ctggtggacg ttatggtctt aacgccgttg atggtgttga cggccccttc aattctgtcg
     4261 gccacatcaa catccatgac atcgggactg gcacctttga gcgtagtggt aatggtgaca
     4321 atggggaaat ccactttggg gaataaatcg acgccgatgc tggggtaact cacaatgccc
     4381 agcaccacca gagccatgat gaccatggtg gcaaaaacag gacgtttgac ggatgtatca
     4441 gcgagccaca tgaaccttta ctccgtccga caaattgttc tgaccgacca ccacaacctg
     4501 ttctttttca ttcaggcctt cgatgatttc aatatcttct ccgtatttcc ggcctgtttt
     4561 aaccgttcgg gatcgtgccg tgttgccttc cacgacgaaa agactgacgg tggcgtcgtc
     4621 atacagcaag gatgtcagag gagcgacaac aacctcgcgc aacgctcccg tgtaaatggt
     4681 ggctctggcg aacaaccccg gtttcagtaa attctcggca ttgacaacaa ccgcttccgc
     4741 ctgcagcgtt ctggttcgtt cttccagatg ggggtaaagc agatgtaccg tgcccttgaa
     4801 ttttctgccg ggaaacgaat caacggtaaa atcaacttcc tggctggtct ttaaatcggc
     4861 cagttctttc tcgccgacgg tgaaattcaa tttcagccgg tcgaccttga ttaactgaaa
     4921 taacggcata ctggtacgca catagtctcc gacagagact cttttttcct tcaccattgc
     4981 acgcagaggc gagtatatct ttgttcgggc cagcctttcc cgggccgtat ccagagtcgc
     5041 ttttgctttg gccaggtcag cctccgccag cgcaacccgg gtggagatgt catcaaattg
     5101 ctgctgggta atgagttctt ccttaaagag agcttccttt ctctgaaatt cggctttgac
     5161 attggtcaga ttggcctggg cctgtttcag agccgcctgc gctctttttt catccagcgc
     5221 ataatcaata tcgttgactt cagccagcag agtgcctttg cccacggcag acccttcttc
     5281 caccctgacg cttctgacaa tgccgtccac ttccgaactg accgtgactt cttcgtccgc
     5341 cgtcaaggtt cctgttgtct caatataagg ccggatgttt ttcttgaccg acgtcgaaac
     5401 acggacgttg gttaagacct ctttctccac gatcttttct ttcttgcagg acatcaatga
     5461 aaaagcgatg atcgctgtaa gtgaaatcag gaatataaac ttgactgtgt tgcgtcggga
     5521 ttcttttttt tcaatgaagc tgacaagatt cttcaaatgc ataacctttt ccctcctggc
     5581 cctaagtctt tttttctacg attcatcaat gctgaaaaac agcaataacc tgccgcccga
     5641 tttttttgcc ctcaaatcgc cgacgtcggt caactgccat aatgcgccgc tggatccttt
     5701 gaccgccgga tcgccgtctc tgacaaactg cagtagcgtt ccgcttgatt ttttcacggt
     5761 cagagaggca agctggtagt tatataaagc atcgaccaca ttcttatctg ctgtcagaag
     5821 cagtgtgttg gcatccatga catcgagact ggttgccaga ccgttttcga attgtttcat
     5881 caccgcgttg taattatccc gggcaaaaat ctgttgatct tccagaaatt tcagggaacc
     5941 tttcaatgtg tccaggtcga gacaggcagc ctccacttca atatccacgc tcttctttaa
     6001 atcgtcgtaa gccaggcggg cctggcgctc tttagccctg gcttcccgga attcagccat
     6061 tctcaagccg ccttcaaaaa acgggaaggt aagcgaaatg ccgccgtaaa tgttttcgtc
     6121 attaaatgtc gagccggcag gatcctggtc cgcccggtta taaatagcaa acaaccccac
     6181 actgggccag aaggcgccgc gggcgtattt gacctgctgc ccggccattt tcgtctgcat
     6241 atcgtaactt ttcagatctg tgcgcaaatc cgatgccgat ttgcgcaggc cctggatgtc
     6301 acacgcttcc gacagggata cggtttcttc cttcagccgg aaatccggtt ccacccccgt
     6361 cagacgcgtc agggcggcgc gcgccagttt cagggcattg gaggccttga gatagtccga
     6421 tttggcgccg gataattctc cctgggcacg cagaagcgcg gttttggtca gttcgccgac
     6481 cttcatccgc gtttcgacca gatggtaata tttggtgagt ctttccagat tggccaccgt
     6541 aatatccaga gctttttggg ctttgagtac gtcataaaaa gaggaggcga ccgccagaag
     6601 aaaatccgat ttggcccaat ccagatcata ttcactcttg gaaatggact ggcccgctat
     6661 tctcaacgca tccagttcgc gggcgctcag cgagaaagtc tgatcggcgc gcacccccca
     6721 tgctgccgag tcttccggct ggatcatgat attcgccgtg ttgtacttgt cttcggtaaa
     6781 ccggttgtag gttccgtagg ccgtcagccg ggggattaaa agcgacaggg ccttgtcttt
     6841 gctcatctgc gcgacataga cgttttcgcg ggcgatttcc attttttcgg acgtattcag
     6901 cgccttctga taaatttcac ggagagtgta ttcctgcgct acggcgtttg cggcagacag
     6961 cagcgccgtc gccagcacag tcatcataca gagcaaactt tttttcatca tgaattcctt
     7021 tcgcaaaaaa tttatggcca gatattgccc atcgcccgtt gcagatgcgc caaacggatg
     7081 ttgtaatcgc ccagggcact ggcgtattct gatttggcac gggttaaaag cgtttgcgca
     7141 tcaagtactt ccgtggacgt ggcgactctt tccttgtatc gttcttcaga tatccggaaa
     7201 ttttcctcag cttgtttgat caacgtttcc gagacggaga tctgattttg cgcctcctgc
     7261 agcaacaaat aagcgttttt gatttccaga gcgatctggt cgatcaattc tttggaggat
     7321 tccgaagcct ggttttctct ggctttgctg gcatcgaccc tgaattttgt tctgccccat
     7381 tcccagaaat tccagttggc gaccgccata atgtaccagc tctccgcatc tttaaaatcg
     7441 ctgccgtgaa cggacgcctc gtcgccgaat cgcgtataat taccgaccag acttaaagcg
     7501 ggataatatt cgctttgcga catgcgcacc agtttgccgg cctgctgaac tttgagcgag
     7561 gatattttca tttccggtct cagccggcgg gccgtttcga ggcattcttc aaacgtctgc
     7621 ttcagcggtt ggtaagccag aatatccatg acgtccacgg gggcggaaat atcgcgtttc
     7681 agcagcgtat tcaaacgcga tttggccagc tccaccgcat tttgtgtccg gaccagggcc
     7741 tgtcttccgt tggccagttc cacgtcggcg aaaagcaggt catttttggg aatcagtcct
     7801 accttgaaat agttttcagc gacatcgcga tgcgctttga gcatttccac cgattgaacg
     7861 gttgcctcgt tgattttctg cgcgcgcaga atattaaagt atgccgcttt gacgtcccgg
     7921 accacgtcct gaatgcgcgc cgtttcctcc aggcgggcaa cctcttcgcc gatgctgctg
     7981 gcctgataat tggccagaat tccgccgccg gcaaaaagcg gttgtctggc ttcgatgatc
     8041 cagttgtaat tgtctttcgt tcccacggga gtttccatgc cgtttaagtc atacatcggt
     8101 cccggaggca gtccctggaa tctggaaaaa ggcgcttcgt ttaaacggga gtaactgtag
     8161 gccgtgctga acttgggtag aaagccggtc aaggcctccc gtttctgagc cgtggcgctg
     8221 ttcaatcctt ctttcgcgat attcaggatg ttgctgtttt ttaaagccag gtcaatactg
     8281 gcttccagtg acagaacctc ctgggcgaag ctcaaacgtg acgaaaaaac aatcatcacg
     8341 actatggtca aaatccggat ttttttaccg atcatgattt ttaaccccat taaaaaaaat
     8401 gtccagaatg aacccctttt tggaataggg ggactcgttt ttcaatgata acatccattc
     8461 catggatacc gccctgatca aataaaccag ggattgagcc atgtcgccgg ccggcaggtt
     8521 ttttaacagt ttttgctgaa tcccttcttt cagcaagttt tcaataaaac cgatatattt
     8581 aaaataatcc ttgatgattt tttcccgcat ggatgtcatg gcttccgaca gagcggtgct
     8641 ctcgcccttg atgaataaac ggcagaaatc ggcattctct tccaaaaacc gcagctgagc
     8701 atcaaccagc atctcgattt tatcgagtac gttatccgcc cgatcggttg ccttttttgt
     8761 ttcttcatac atcaaattga gtttttcgct gatcatggat gagtaaagat tttccttgcc
     8821 ctcaaaaaat tggtagagag atcccgtgga aaatccggag gcgctggcga tttccgccat
     8881 ggtgacatta tggtacccct tgacggaaaa tattttttcc gctttttcca gaatctcagc
     8941 ccggcgcatg ttgaattcgc gttcttttct ttccagtctt tccatcgcaa aaactcacgc
     9001 tgtgaataat cgttcaaatt ttgaattgcg gttcactttt tttatagcat attgacgatt
     9061 tgtcaagcca aatatttcgg ataatgcaat taattcatcg ggaaaatatt aaaaacttct
     9121 ttctattatt ttctgaaatt taacgtttct ttaaacatca tagctattcc aggaaaattt
     9181 ttttccctag cgaaaaaaat tattattctt atattttaat agtttataaa ataataacgc
     9241 cttttatgca ttttggcata cttatttcat taaaaatgac caagagtttt caagatcatc
     9301 gaattaagga gaaaaagagc tatgagaaaa ttaatgcaaa ccaccctctt gaccgctttt
     9361 gtagttctgg taaccacctc tttcagttat gctcagtaca gcgttaccgg tgatgccaat
     9421 tttccgttct ttcaccttgg ttgtttaatc gttggcggtt tgatcatcat ttccctgaaa
     9481 agaaagtaca ccaagttgta tatgagcgaa gccatcggtt cttttgcgct ttacgcttta
     9541 ctggtgtcca tttttacggc gccggtggtt gatgccctta aaaacttgat caactaaccc
     9601 cttttcccct tcaaagatat acccttcagg aaccccgcgc agttcggcgg ggttcgcctg
     9661 tttgtgccac gcgttttaaa gaagttcatc cagccgggcg gagagctgtc gggacagttt
     9721 tttcaggaga cttgtctcca tatccggaga gtaacggttc tgaatggcat cgccgttgtt
     9781 ccagctgccg gcaacttctc cgttgatttt gaacggaacc atcgccagcg attttatgaa
     9841 atacttctga tttggcggaa ataacctata gtagggcctt aagtttttat tgaccagaat
     9901 cggttgcagg ccggaaaaga agatttcacg aaattgatcg gctttgatga cattgagcct
     9961 gtcctttaaa atacgggatg atttgaaggc gtcgatgacc cggcccgttt ttatggtatc
    10021 ggccacgctg atccagatga atggcacctg aaattccgct tcgatctgcg taagcagcat
    10081 ttcaaaaagt tcggcaatgc ttccggctcc ggacaatttt ttttcgactt tgtcaaaacg
    10141 gtcggcaatg tttgtgttga tgcgttcaat attttctttt ttatccattg cggcccctta
    10201 ccactgcgcg taaagaatca ggtgatcgga tggttttttc gccagtcgtg agtccagatc
    10261 gatggcgctg ctctttgacc tttcggccag tgcttttgtt gccaggatgt gatcaacccg
    10321 ccagccgagt ccgcgtttga ccgaatccct tatacggtaa tcgaaaaaag tatattgacc
    10381 cgcgattccc ggatggtgtt tgcgaaacac atccgtcaat ccccatgatt tgatgtcctc
    10441 gagagctttc cagacgtccg gattgaaaca gacatggccc agcagtcttt tcggatcatg
    10501 aacgtcgatc cgttcaggcg cgatattgag atcgccgcac cagatcagtt gattttgcgg
    10561 ccggaaattt ttatgcagat attttttaaa acgggcaagc cattccagct tgtatgtaaa
    10621 ttgggaactt tctatctcct gaccctgggg aacgtaagta ttgatgatcg tcagatcgtt
    10681 gaatgcggca atgatcatac ggtcggggtc ggacggctcg ctgtcaaaac cgtaaatcac
    10741 cttctgcggt ttttgttttg agacaatcgc gacgcctttg tactgcttgt cgcctttgaa
    10801 aacaacatga taccccaggg cttcaaactc tgccgcgggg aaaaccgtgt cggcgacttt
    10861 cgtttcctgc atgcagaaaa agtccggact gtgagctttc agccacggaa caatgatgtg
    10921 catacgggag cgaatggaat tgacattata agtcgcaagc ttcaatggaa tgtccctttg
    10981 ttgatttttt taattaatat catgaccggg cattgcactc aagttttttt gcttaaagct
    11041 accgtagctt ttttataaat ttgtcgtaaa aaaagatgtt cagaaacaca tccatctcat
    11101 cggggcagca tgcccgagca taaaattttt ttctgttctt tcctgtagaa gatgccccgc
    11161 aacgttgcca gatgttcttt ctcccgattc atcaaggtga aattatgctt tatgacggaa
    11221 tccgtcaact atttgcggtt ggttttagca tgaaaataat tttcttttcc gttgacagcg
    11281 cagcctccct tcttttataa acaatgggga aaaacaggtt tcccgaatgc ggtttcgtga
    11341 aacatcatct tcccttatca tccttttggt catttcttgt cggatatttt tgatgacggc
    11401 gatgaagcat cgaaaatcaa aagttgaggg ttcatactca acaggctgtt caaaaatatt
    11461 tatggagaca ttatgcaact catagaatct gttctgcagc aaataagcga tatcctctgg
    11521 ggctggcctc tgattgtgct tctggtcgga acgcatatct ttctgacgtt ccgtctcaaa
    11581 ttcattcaac gctatatgtt caaagccatc aaaatgtcca tttcacgaaa gcgcgaaggg
    11641 gagggggaca tcagccagtt cggcgcgctg accacggccc tggcggccac gatcggcacc
    11701 ggcaatatcg tcggtgtagc gacggcgatt gccgccggag gccccggcgc cgtgctgtgg
    11761 atgtggctga ccggcgtctt cggcattgcc acaaaatacg gcgaggcggt tttatcgctg
    11821 aaataccgca tcaccaacaa agagggctta atggccggcg gccccatgta tgttctggag
    11881 cgcggtatga acgcccggtg gctgggattg attttcgctg cgctgaccgc agtggcggca
    11941 ttcgggattg gcaacatggt acaggccaat tcgattgcgt cgctggtgca ggaaaccttc
    12001 aatatttcac cgtggataac cggactgacg atgactgtgc tgacggcggt tgttatcctc
    12061 ggcggcatta aatccatcgc gagggtctgc gaaaaactgg tgccttttat ggcggtgttt
    12121 tatgtcttcg gctgcttgat tttattgggc atgcattatg aaacgatacc ggaaacgttc
    12181 aaactgattt ttcaaagcgc tttttccgga catgccgtca tcggcggatt tctcggtgcg
    12241 ggcatgaaag aggcaatccg ctacggcgtc gcgcgcggcc tgttttccaa tgaatccggc
    12301 atgggcagcg cgccgattgt agcggcggcg gcgcagacca aaaatgcaat tcgtcaggcg
    12361 ctggtttcgt caacggcaac tttctgggac acggtagttg tatgcgcgat aaccggtctg
    12421 gttctggtca attccggcga atggattcac ggctacaaag gcgccgcgct caccaaggcg
    12481 gccttttcgg ctttgccggt tgtcgggccg tttgtgcttt ccatcggcct gctgacgttt
    12541 gttttttcaa ccattctggg ctggtcctac tacggtgaaa aagccgcgga gtatcttctg
    12601 ggaaccaaag ccattcggcc ttacaggtat ctgtgggtag gcgctgtgat gctcggttcc
    12661 gtcctgagtc ttcccattgt atggaccttc gcagatatcg ccaacggatt gatggcagta
    12721 ccgaatctca tctcactgct tgttctgaat aaggtgatta tcagcgaaac aaaacatttt
    12781 ctctgggagg gtaagctgga tgaaccccgc tgatttgaaa acgaaccggt aacgttttgt
    12841 ttgaaatcag tccaacgaac aataaaccct ttccgccgga ttgcttcctc aacggaagga
    12901 tccggtttat ggtcgcactg aacggattcg tcaaactgtt acagtgacga ttcctctgtt
    12961 gaagtcccgg atgtgccatg ggattgtcat ttgaccacgc ccggtgtgcc atatatcaat
    13021 gctgtccatc tcaagcggca ttgtcttcca acacgattca caataatacc agggtgcgaa
    13081 aaccttatcc acacaaggag gcacccgtca ccgaggcggc aacgattctg cttgatttga
    13141 tccgaatatg gcgccagcga ttaaacggaa catggatagc gatcccgctg atccgtcagg
    13201 aaggagaacc ggtcggcttc tgactgacaa gggattcaat ggctttgcgc ttgatgccgt
    13261 ccgttttctc gataacatcg acgaattcgg ttttgatcga gacggaatcg tcaagcagac
    13321 gacgcaaatc ggccagcagt tcttcgagat gggcgctgtc atactgattt cctttgacga
    13381 ttttgatgac gattagatcg ctatgcacct gtgtcacctt gaactcctcc agcagatgaa
    13441 ggtacttctt gatatacatg gtgaaggtgt tcagaagttt cagcgaggag acgaattttc
    13501 cgctcggaag aataaagcaa tcgtcggccc tgccttcgaa ggattggagt agcgggaacc
    13561 ccatgccgca gggacattca gcgttgctga atcttcccat gtcgccgata cggtagcgaa
    13621 tgaacggcat ggcagggctg cgggtagtcg tcagcacgat ttctcccatg tctcccgggg
    13681 caacctcctg acctttcgcg tcgatgaatt ccacccatac attgtccgta aagatatggt
    13741 agttgttgca ccggcactgg gaggcgatca tccaggtctc ttccgtcgag tattcatcgt
    13801 agacgggaca gccgaaggtt tcggagatga aggtccgctg ggcctcagtc gaaagctccg
    13861 agttgattcc gatgaacttg ggccggatat ccttcaggtc atcaggggtg gcgtttctcg
    13921 cgatctcgta caccagagag gcataaccga tgatgaggtc ggcccccgcc ttcctgagct
    13981 gcgcgatctg ttcttcgacg gggatcgtcg atttgatatg gacggcccgg aagaaagggc
    14041 ccagacgggt atagttcagg tcggtatatt tgatgtaggc cattttgtgc catgggcggt
    14101 atccgatcat tgtgtacatg cggaccccca gggcgacgta gaaggcatag gtcgcctgag
    14161 tgaagatgaa cggcagggac ctgcctgtgg atccggtggt ggccgaatag cggcacgcct
    14221 ctctgttgac gccgcgtgcc acgatcccgt cgggaaaatt ctgccggatt tcttccttgg
    14281 tgacgaaagg gagccgtttg atgtcatcgg cggaccggat agatgtaatg tccactcccg
    14341 ccgcatcgta tttctggcga tagaagggga catggtcata agcatgccgg atggagcgaa
    14401 tcaagcgtct ttcgcatagt ttcctctgga gtccataggg gagacgggcg ctgaggtgga
    14461 tttcaattaa ggatttgaaa aagtgataca gcaatatggc cgtcttcatc ataacggata
    14521 cctcctagtt attgttattc tcttttttat tctttccgaa aggccggatc cagggactgg
    14581 ctccccggct gcctaaccgg cggatcatat tttctttcca gtcgtcgaat atgtcatagg
    14641 ccgccgggac aacggccagg gttaagaaca gagaagtgag aagccctccg atcagcgtaa
    14701 tgcccatggg cgctcgcaga tcggctcctt ctccgatacc gagggcgaca ggcagcatac
    14761 cgaacaccat ggcaaacgtg gtcatcagga tggggcgcag tcggaccggt cctgcctgaa
    14821 ggatggcctc ccgccgtgac aggccccgct cacgcagtgt gttcgtaaaa tcgaccagca
    14881 ggatagcatt cttcttgaca agacccatga gaagcaccag gccgatgaag ctcacgatgc
    14941 tcagcgattt accgaagatc aggagagccg caaaagcgcc gataaacgac aggggcatgg
    15001 acaggagaat ggtcacggga tgaagaagac tttcaaactg cgaggccagc accatatagg
    15061 ccagcaaaat tcccagcagc agggcaaaca taaggtactt gaaggacttg cccatttctt
    15121 cggcctctcc ggagtaaccg gcaacgtatc cagccggcag aatcctggcg gatatggcgt
    15181 caatttcctg catggcctcg cccagcggct tgtcctggag attggcgaat atccagatcg
    15241 tgcgctgccg gtctttgcgc gaaatgctgc tcggaccgcc cccttcctcg acggtgacga
    15301 tgtcacttaa tctgaccagc tcaccattca cggcccggac atagagtctg ccgatatcgg
    15361 acgggctcat cctgtctttg gcgtccagcc tcatccggac atcgtatcgt ctcccctttg
    15421 ctgtatcttt gaattttgtg acctcgacct cgccggacat gagaagattc accgcttcgg
    15481 tgacggctgc cacgctgact cccatattcg ttgccttttc ccggtcaatg tagacactca
    15541 actccggttt ccctgcctcg atggaggtgt cgatatccac gagccccttt attttggaaa
    15601 gttcaccgac gatttgcttg gcatattttc tgagctcgtc gatggatccg ccgctgatgg
    15661 aatattgaac ggggacattc cggatcccgc ctccgagaat ggcaacctct tcgatggtgg
    15721 ttttcagccc ggggatcgcg gtaaattcct tccgcaattc cgccataatc tctgactggg
    15781 atttgtctct atcagaccgc ggtttcaggc tggtttggat gaatgcctga ttgatttgtg
    15841 acagtcgccc ctgacccgct ccctgaccat agaagaccga tgtcacctcg gttctgttcc
    15901 ggacaatctt ctcggcttga ctgaacatcg ccccggcgtg ttccacggaa taatcgatgg
    15961 gcgattccat ccggatcata aactggctct ggtcttctcc cggtttgaat tccttgccga
    16021 tgaaccgggt aatccagagg ctgaaaataa agaggatgag ggcgctcatc agcacagccc
    16081 ggcgatggtc aagactgaat gtcaggatgg ggcggtaggc ctcttcaatt tttttatatc
    16141 cgcgctccag cgtctgcccg atccgcgcca tcaccgtgcc cgggcgttca ctgtccaagg
    16201 attctttttt ccgcgggtgg agaagaaggg acgagagcat gggcgtgagg gtgagcgaca
    16261 cgagcaggga aacgaggatg gaaaagacga cggtcagcga aaactgcatg aagaaacgtc
    16321 cgatgatgcc tttcatgaaa gcgacaggca ggaatatgac cacaatggcg agcgtggtgg
    16381 ccatgaccgc gggaccgatc tcgtccgtgg caaaggacgc ggcctctctc ggagacatcc
    16441 cgtcttcgat gtggcggtag atattctcga tgacgataat ggcatcatca ataaggatac
    16501 cgacagacag ggtcagggcc agcatcgtca gattgttaaa ggtgaaatcg aatatcctca
    16561 tgatcgcaaa ggttgaaatg atcgaaacag gcagggcgac cgcgctgatc agggtcgtgc
    16621 ggaagtttct caggaagatg aacacagcga taatggccag gatcgatccc agaatcaggt
    16681 gttcttgaac ctgatccatg gattgtttga tgaacacgga ctggtcgaag gcgatccgga
    16741 tatccatgcc cggcggcaga cttttcttga tgcgctcgac ttcctctttg acacggtcca
    16801 caacctgaac ggtgttggta ccggtctgct tctggatacc gataccgacg gcggagatgc
    16861 cgttgaaccg tgcgtaggaa cgtctttcct gggttccgtc ctccactctg ccgatatctc
    16921 tcaatctcac gggaacgccg gcataggagg cgatgatcag atcattaaag gcggagacat
    16981 ctttgagaga ccccttgact ttgatggaga attccttcgt caagctctcg atacgcccgc
    17041 ccggcaattc gatgttttcc cttttcaagg cagccgcgac atcgcctggt gaaaccccgt
    17101 aggccgccag tttgttgctg tcaagccaga cccggatctg gcgtttctgc atgccggcca
    17161 gggagagggc gccgacaccc tggatttttt gtaacttctc cttgaggctt tcatccacat
    17221 aggttgagag ttcgcgttga gacttgtccc cggtcagggc cagccacagg acagggctag
    17281 catcgatgtt gaccttctgg atgaccggct cgatgatgtc atccggaagc tgtgaacgga
    17341 cggcggagat ctgctcccga acatcctgga cggcaagatc gatgtcccgc tccaattcga
    17401 actccacgat gacactggac accccgtcaa tactcagcga gtggatcgtc tttaccccgt
    17461 taatcgaact gaccgcctct tcgattttgt ccgtcacatc gacatccata atctcggggc
    17521 ttgcgccggc cagaccggtc gtgatgttga cgaggggaaa ttccaccttg ggaaagaggt
    17581 cgacgccgat tgacgggtag ctgaccaggc ccagaacgac gagagcgaga atgaacatgg
    17641 tggcgagaac aggccgcttg attgaggtat cagaaatcca catgatgtcg tatcatcctt
    17701 tcttccggtc cgccttcgag gcaaccagtc cctgttgaat atggtccatc agtgtttcca
    17761 gacggccgcc gccggccaga acgttcggca tggaatagat catcgtcata tcatcatcac
    17821 tgttgcacat gccgataaaa atgggggagt attcctggcc cgggggaatg ccccaggtat
    17881 attttccttc aaagccccct ccgctgaaaa ccggaatccg tcccatattg gtgaggaccc
    17941 cggagttggg gtagcggccc gtgcggttct tggtggcgat attgctttta atcagttttt
    18001 tgagcaacca gaggggtaga aagcgaatcg gccggtcgcc ccagtaaatc atgccgtcat
    18061 gccgcgcttc cagctgacgc ttgatgtcct gactcacgtc agccggtgtt gtggagggat
    18121 tgatttccac gtaaatcaga ttggtcaaat tccctgtcga acgcaggccc tcaaccctgg
    18181 cgcgcatatc caccggaata gccaggcgca ccttaccgtc ctgatactgc caggcttttc
    18241 cggccaggag agtggccacc tggcccagga cattgtggta gcgtccgggg tagcgccgtc
    18301 ggcgccagat tacgccgggc tgatctcctt gaggcatacc ggtgggagcg atgtattccg
    18361 gaaccggccc tttgcgtcct tgcttctgaa aggttgccgc aatctcaaat tcagtgatgc
    18421 gcgaatccga tcccaaggga gcttcgccgc gcagaccgcg gaaaatatct tccacccagg
    18481 tcacggtgcc ccggccgtcc atggtggcat gcatcgtgcg aaagacaacg cgcagggggt
    18541 tgccgtggat cagaacaact tcggatgtcg ggccttcaaa aggattcagg gtttctgaga
    18601 gcttggccgg agcgccttcc ggaccaagac cgtcccaatg ggttccatcc acctcgatca
    18661 ccggcggggt ttttccggta tcgacgagac gggcaaaggc cgtgataccc ttgacaacga
    18721 cgcgactacc cgggttggcc tcggatgcga tttttacggc ttcccgccat ttgggcaggt
    18781 cgaaatatcc ggttccttcc caaaataact ggttgagaaa cggcggacag cattcattgt
    18841 acaccacata tgcccgctcg gtggaactca gttgccgcga gtaagtcata tcgtatcctc
    18901 cctttggttt gtcccttcca gtccatgaac gattctcttc agggtgtttt caagccggcc
    18961 ttcactggcc agaactttgg gcatggaaat cgtcagatct gacccccctt catagcccgt
    19021 catgaccata aaaaacggat agtattcagc agccggcgga ataatccaga atgcccgtgc
    19081 tgtgaacccc ccgccgcaaa accggttaag attaatccgg cccaggttgg acaggatgcc
    19141 cgaaagactg taaagtcctt gccggtgccg ctcgcgaatg atattttttg ccttgtatcg
    19201 catcatccac aggggcatgt attccagacg ctcgtctccc ttggtcagtc tgcattcatc
    19261 gcatcgactg atttgcaaag cgatgtcccg ggcgatgtcg tccgggctcg tttccttttt
    19321 gacctccgta tacagggcaa aagatagatt ggctgtactg cgcagttccg gtctgcgatg
    19381 tcgcaaatcc acggggatac cgaaacggac aaccccttcg ccatgtcgcc aggcttcttc
    19441 cgctgccaga cgggcgcatt gtgccagcag atggggcatc ttgcccttga gggaacagcg
    19501 tctccaggtc acgccggtat cattgccttt tgcccgtccg gtgggggcga tatgctccgc
    19561 ggggaaaggc gtccggtact gattctgaaa actcttacaa agctccacat ccgtgatgtt
    19621 tgaggttgaa ccgacgacag cttcgccgcg cagggtccgg aaaacatcct cggcccagaa
    19681 cagcgtgccc cgtccgtcac acacggcgtg atgggagcgg aatgcgactc tcggggtcgg
    19741 accctggatc agaacaatct cgcaggtggg gtttccccga taggggagag actcttcgag
    19801 taatggttca ttctccgggc cccgtccgtc ccagttcctg ccgtccactt cgcggacgcg
    19861 catgtttatt cccgagtcga cccatcggct ccagctcagg tgacccctga ggaccagacg
    19921 ggtaccggga ttggcctcgg aagccgcatc cgccgccctg cgccattttt ccggatcgag
    19981 cgtcccttca ccgtcgaaaa taaattggtt gaccgtgggg gggctgattt gattgagcac
    20041 cacaaatgcc cggtcattaa acgtcatttt ccgagtatac cgtttcgtca tcgcgccggt
    20101 ccccctgacg atttcttgtc tgcatcggaa gttgccctct tgggtcccgg accggaggct
    20161 gattctgaaa tggtcatggt ggcgcgcaca atggaactga caagcgcggc ggtggctttc
    20221 aacgcctcag gagttcgcgc cgggcggggg atctccatgt gaagcgcacg ttccatgttc
    20281 cgtgtggaga gatatccggt cgttttgttg cagcgtgctc cgaagtagat cttatccagg
    20341 ggctctatgc gcagctccag atcggggtag gccggatagg ctgcctttgc ctgcgcaagg
    20401 aaagcgggaa agcggtcttt caggcgccgg gcttcttccg ttgtgattcc tgtggtggcg
    20461 atttcaagcc ggttgagaaa agggatgctg gtgtgtccgt gaatttcgac ataaagccgc
    20521 aacggcttgc cgtcacggat cttgtcaatc aaacgagcgt attggtcgta aacatccctc
    20581 gccctttcgg tacgccgttc tttttcgcac gtatgtcctg caccctccga ggggcggttg
    20641 acgttgatgc gcggagatgg cgacgcaaaa cctccggcga tgacatagcc tgcaccgagc
    20701 gtccgggcca catctttggc caggacatcc gtgcggatgt cataggttcc atgcggtgcc
    20761 gcaatgagaa tgccctgcgt cgtcgatagt gtttcaaaat gaccgagagc atgtaaaaaa
    20821 tgctcttgtg cgacagcggt ttgaatactg aaggcggtga ccagcatcac aaccgccaga
    20881 tgcaatgccc gggacatcgt ttggtcgcgg ccattctttc ttttacggag ggcgctgatg
    20941 agatacaggt ttcctctagg ttccttttga agacggggca ctgattgatc aaggggatat
    21001 ggtgcaggcc tgccgatcat cctcaattcc tttgttcact tgtgctgtcc tctcccgcag
    21061 gaagaccctt gatatgtttc gcatagagca ggccgatcag gtaacgcaac accaggatgg
    21121 aggtggtgta gatcagtccg aaagccatga tattcagggg cgtcccgtaa tccttgaaga
    21181 gaaggagcat cgtcgcctgt gccgtgccga atccgcccgg agcgatgggc agactgatga
    21241 ccaggagaat gatcgggaca taagcgatga gcgcaaccag cgggatttgc atgttgaatg
    21301 ccctgaccgc aatatagaag aaggcgacaa agcaggcata gtagagaatg cgccagaaga
    21361 tgaggacacc gtaggttcgc cagggagcct cgtgaaagac cttcagaagg ttcatgcttt
    21421 taattttgag ggccagaccg cgggacggca gttttttcaa cacgataata aaaagaatat
    21481 ccagcagggc aaagccgatg aagaagatca gcaaattacg cagcaatccc gaggccggag
    21541 aaagaatcac caagccgatg atggccatgt aaaccagagc cttctgggtg atgccgttga
    21601 aaaaggtcat cagtcccgtt gatcgcatga ccgggatgcc gagatggttt ttcagatagt
    21661 agatcagaac tcccatgccc acactgtagt cgatcgtcat gatgagatag gtgacgccgc
    21721 gcagagtgaa ggcatttttc caggaaaggg ggtaatgaaa atgcttcagc gtctcagcca
    21781 gattctgtgt gtccagaaga aaggtgatca gaatgagggc aatcaaagag ggaatataca
    21841 gggacacatt cgcggtcagc atggcattga aacacatgcg gatatcaatg tgccggaaca
    21901 aataaaggag gattgcgccg gagactgcca gagggagcag ctgtttccat agtttggtat
    21961 tcatgaggaa agggcgtgcc ctttcctcat gtggaaaatc catatccccc atcaccgctt
    22021 gagagtttga tatcttggct gtcttgtcca tctaattccg tcatcgaata cgtcttcagg
    22081 ggactctttc aaaaatatga ccacgggagg gatcttcgca gtgcccctcg tccatcatca
    22141 tattagtatt gccagggaaa ggcatccgca gagccggtct cgaagttttc ataagcataa
    22201 tccgcgcagt aatccgcaaa atcctttaga ttgaaagcgg taatctgtgc cgggttgctt
    22261 tggagagaag acaggaacga ttcggcatta tcaatgatcg tatcctcgat ctggaatgtc
    22321 cactgctgaa ccttggccag ttcgacctta ttgatgtagt tatcgtacat gccccagatg
    22381 acatctcctt cattcgatga ataaatgaga ctgccgtagg tttccctcgc gctgatggcc
    22441 tgattgagca gacaggaatc atcgtaatct ctccaggcac cgttggcata cgtatccacc
    22501 cgggccagtt ccgatgttcc cctggcggcg gcaaaaaacg ggacgaaaac acctacagag
    22561 ggctctccga ggttgaccca catggtggcc aggcgcgggt tgtctccgga tgcaacgccc
    22621 ctgacgacga tcgagctacg ggtggcattg cggctgaggc aataggttgt gctgtaattg
    22681 gtctcgctgg aaagatccgt ctggacctgc ttgccgacaa cgtccttggc cacctctcgc
    22741 ataacggtgc ggtaattgat ccgcttctct gttgccaact gggtcaggag ggcttcggct
    22801 ctatacttgc gatcctgtcc cacgttccag ggaatgtagt aattgtaatt ggtcataatg
    22861 tggaatccga tgaagttgct ctgagcccgt ttgagcgtcc atccatactg attggtgatc
    22921 ttgcccgtgt tggcatcgtt ctttctgaac tgcatcaggt tgaggccggt gtttagatgg
    22981 ccggtgaagc attcatacag tgcggctccg cctttggcgt cgatgacaac gaagttggcg
    23041 ctgataacgg tgccccagtg atagtaaggc catcttttga gcagtgattc aaactccgcg
    23101 agcgtggtac atgtcgccag ggcttcctcc ataagaacgg tattgatgtt caccggttcc
    23161 gccataagat tataatcctg gtacacggac gtgccggcaa tggcaaaacc tgattcattg
    23221 acgccgccgg aaggagtgat cgccgagccg ttgttcagca gtttcatccc gttttcaaac
    23281 cggtagacca ttaaatatcc gccgggaccg gactttttgg cggggaagta ggcaacctgc
    23341 tggcggtcgc ttgacgagtt gtcaaagttt ttccagatca gcggtttgcc gtctgtggtg
    23401 accttgccgg agacaacggc cacatcacag gcccatgtcc gcgtcgcgaa gataagggcc
    23461 aataggcaca tcaagacgca ggaaattttt aaataaccct tttgtaactt aatcattgga
    23521 gcctcctcat ttactggttg tttcaaacgt taattctctt acgtttagtt ttgaatatcg
    23581 tatattcttc tgcaaatcat tttatccgtg atccgcagtt ttgactgaaa aaggttctcc
    23641 ggacagaccc gggacccgcc ggtcggtaaa agacgggata aggggtcttc tgctgctgaa
    23701 cagtccgaca cccctgctgg aaaagtattc cttgccgaag catttgatca tcagaacgaa
    23761 ggtgaccagt cgtacgattt tcatggggat catcagcata aaggcaaatg cgtcgcccct
    23821 gtccatatgt tcgaaatccg ccggcatgtt tgcccggaca tggggcgaga attgatactt
    23881 cggatcattg ggatcaaaac cgtttgagcc gggaaacatg ttcttcaggc agaaaggata
    23941 gctctgatcc acaagtccga tcccgtattt acggcaatac tcttccttca gagcgattaa
    24001 tgcgggcgac ggggagaaag aggagtcaaa cagtacccgc agccatgatt tattgaaata
    24061 ccggttgacc actgcgggat tagccgagat ggacgtaaaa atgaaataat ctttgttctt
    24121 gattgaagcg gctttcgtga ggtgccattc caccgtcata atgaccagac gttgagcaag
    24181 gtctcttccc cggaatgctt tgagcaccat cgtttcttca ttattgtaca cgcaatgggg
    24241 accgctgttg agaatggaca cggcatcaaa ggcgacgatc cggccgtttt tttccacata
    24301 gctaagaaca tcgcatttcc gcagataggt tgagaattcc tcctgcgcct tccagcaatc
    24361 attttcaacc gcttcacatc cccgaatgat atctccgaga cgattccatt cggctgtatt
    24421 caaatcgatc aggtagtaat tgaatccctt gtggctctga atgaaaaaat cctgagcgtt
    24481 taccggtagg gggtccgttg aatcgctttg atgccccctc tgctcacttg ctgacgaaac
    24541 agacatgctt ttcatgtgta ttcctcctct taatgatgca gttattctaa agagtttgaa
    24601 tgccaataga tgacacctat cggctgacac ttttcgtcgt caaatccgat ccgttatccg
    24661 gacagatttt catggacgaa tctggtttag atttttacta atctctggtt gaatgccggg
    24721 tctcttcaga taggaggcaa cgtaccgctt gatcatgaac gcgaaacaac gtactgcttg
    24781 gtattattgc ataacaactt agtaaatata ttctataaag agcacgcaaa cgacctcttt
    24841 atgaataatc tttcacaaaa aacgcgatat cctcaaatta atcatttgaa ttgaatgttc
    24901 ggtcaatagt atgattgatg ggtaaagtca ataaaaattt tgtcatggaa taaaaaaata
    24961 tcataatcaa gagagcatgg agtggaaata accttctgaa attctagatt cgtcaatatc
    25021 ttcgcacatt taagcgaagg atgaaatgcc ctcaataaca tcctgacagg tctgtccaat
    25081 ttccacgggg aacaaatttc ataaccgggc atcgaatttg agtccagggg tattcacgat
    25141 aagaaatacc cggaagggaa acaggcattt tcattgagct atcccgacgc taaaagcttc
    25201 agaaaaagaa gcgctttcgg gttttttaca ataggtttac attaagtaca tttcaaacgc
    25261 ccgctgtttt tcagaaacga agaaacccgc agggatcgtg aagtttctgg aaggcaatgc
    25321 gtccggtctt gaagaccaat ttgccttatt gcggttttgc tgcgggggat ctgtccatac
    25381 ggattttgca tatcgatgtt cgaaaacagc ctttgatcat tccagacgac atatccgtca
    25441 aagccatgcg ccggaaaatc gtataatttt attcattttt aatattttaa agacgtgcta
    25501 taatgcaact taatcagata tctcaaacat tatgaataaa agaaaaaagc tgggtgaaat
    25561 cttaatcgag gacagcattg tttccgaagg ccagttgatg agtgcgcttg cccttcagaa
    25621 gaaaagcggc aagatgatcg gtcagacgct tattgagctc ggttacatca cggaagcaca
    25681 gcttgtcgag gctttccgaa agaaattttc tatttcctat atcgattgtg atggcgtggt
    25741 tgtgccggaa aaatttctta aactggtcag tatgaccatg gccgttaaga aaaagatcct
    25801 gccgaccgat gtgtcgggga atacgctttt cctggccatg gtcaatcctc ttgattattc
    25861 caccatcgat gaagtgaaat ttctcaccgg aaaaaatatt acccccctgc tgaccacaga
    25921 atcgtcgctc atgaaagcca ttgagaaaaa ttacggctat tgctccatcg aaaaaatatg
    25981 gaagccgttg aacgatatcc atctggacgg cgaaatgcag ttcaatctgg ataaaagtat
    26041 cgagttccat aaaatggaag aggaagatga aagcgcgctc agagccgatg acctctataa
    26101 aataggagaa gaaccgccca ttgtcaaatt ggtgaccctg attctggtca atgccgtcaa
    26161 atcaagggca acggacatcc acatagagcc ccatggaaaa tatgtcagtg tccgctacag
    26221 aatagacggg gacctgtcca ccgttctcaa aatatccaaa acgtatcagg aggcgatgac
    26281 ctcccggatc aagatcatat ccaccatgaa cattaccatc cgccggatgc cgcaggacgg
    26341 caggagtacc gtcaaatatg aaggacgcat gatcgatctg cgtgtttcca ccatcccgtc
    26401 ggctcacgga gaaaccattg tcatacggat tctggattcg gtcaagggac tcatcccgct
    26461 gagcaagctg gggatgccgg ataacatcat cagagatctt ctggagatgc tcaacaaacc
    26521 tcaggggatg ctgtttgtaa ccggtcccac aggcagcgga aaaaccacca cgctttattc
    26581 cctgctgaca cagctttcgc tggaatcgga gagcgtcgtg acgatcgaag acccgatcga
    26641 atacgagctg gagaacatca agcaggtgcc ggtcaatgaa aaagtcgggt ttacgttcgc
    26701 cgcggctctg cgttcgattc tcaggcagga tcccgacgtc atcatggtcg gcgaagttcg
    26761 ggattccgag accgctcaaa tttccacgaa ggcggcgctg accggccatc tggtactcag
    26821 ctcggtccat accaatgata ccattgcaac catctcccgt ttaagggatc tgggcataga
    26881 accttatctg atcagttccg ctctgaatgc catccttgca caaaggcttg tgaaaaaaat
    26941 atgtgaaaac tgccgggtgg aattcgaaat accgcagtat ctttttgaga actaccctta
    27001 tctgaaaaag ctcaaaatcg gaatatccta taaggggaaa gggtgcgaag catgcggctt
    27061 tacgggctat aagggacgga tagggatttt cgaattcctg cgggtcgacg gcaatctgag
    27121 acggaccatc cccctgtatc attccgatca ggacctttgc atcgttgccc gcgaaaacgg
    27181 catgaaaacc ctcattgaag acgcttgtga aaaggttgaa cacggtttga ccacgatcaa
    27241 tgaagttctg acaaaaatac actactacga atagaatttt tttcgggaaa ccttctgttt
    27301 cgtctcaacg cttaggctgt cgcagctctt caaaataagc atccgtcaca ttatccagca
    27361 gctgcagggt ggtcggccgg acaacaatca tccgggtgtc gggatgattg aaaaaatccc
    27421 tcaggtcggg atgcttccgg agaagcgttt cccggatttc ctcaatactt ttatcgttat
    27481 ccacagcctc aacgatgccg gtgaccgtca atgcccgggg agttccttcg gcatcgacaa
    27541 cacgcgtatc gatcagcaga ctgacattgg gattggcgat gagattgtga tatttttttg
    27601 tatctttgaa ggtggccatg tagatttcac ggccatcccg ccctgatgca tacgacatca
    27661 gagaacaatg cggggcatcc cggccgaccg tggccatcac gcacatcctg ttttcctgaa
    27721 tgattttttg aatgtcactg cgcatatcct ctctcctttc ttccggcact cggagtaatt
    27781 ttagcataaa ggcgacggaa aagggcagaa cttttaacgg ccaacgcctg caaaacattt
    27841 ttgacagacg gatgaaccct gtgttaaaaa cgatacgtga aaaacaaaaa acctttctta
    27901 acagaagtca ggtcgcagga cgttttatcc gtttccattc ccgatacccg gtcgtttgcc
    27961 ggagcccaaa gaaaatcgtt ccgtgtctgg tggattgcgt ttcgctatca ttttgttccc
    28021 gccagttttc ttccggctat cctggggtct gtcggcgcct gggtgcagac cggtctcatg
    28081 aaccgatggt ttttctttct cacggtgctg ggcgtaacgc tcaatcacat cgccctgaac
    28141 atgaccgacg actattttga ttatcacgcc gccgtcgatc aaggcggtga acgccgggaa
    28201 aatccttata ccggcggcag cgggaccctg accggcggcc tgatcaaaca cgggcacatg
    28261 cgcctgatgt tcacgttatt ttacctgatc accatcggga tcggcctcta tctgtgcgcg
    28321 gcgcagacct ggtggatcat cgttttcggc ctgttcggcg tggggtgttc gtatttttac
    28381 acggcaccgc ccatccggta cggatatcgc ggcctgggag aattctccca gttgatcaat
    28441 ttcagcttta ccatcggcat gggcgcctat gtcgtgcaga ctgtttcttt ttcgtgggag
    28501 attttctgga tcctgttgcc cctggggttc atgatgtttg ctatgatcgt catcaatgaa
    28561 atcccggaca gattcgacga tgccgcggcc ggtaaaaaca cgctggtggt gcgattcggc
    28621 ctaaaggcgg cagcccggtt ttacggcgtg gcaatgatgc tggcctacgc ggtcattctg
    28681 cttgcacctc ctctgaacat ggcaggctac tggatttatc tgggcttgct gaccctgccg
    28741 tggtccgttc gcgcattttt aatcatgaaa gcatacggca acgaaccgga caaactggcg
    28801 cctgcgaatt tactgaccat tcgcgttcat aatttcaccg gaatcttgtt gatcattgcc
    28861 tgcctgattc aggggctcca ggatcgtcaa gtgtttcaac cggttgtttt tgccgcggtc
    28921 atcctgtccg ctttttatct gccggcggcg atcagtattt tctttaaaaa aatgccggat
    28981 ggccgccgca ctgaagcatg ataatatcca gtcgcattcg gtcttgaaag ccgggcggga
    29041 aaacattaca atcattataa ttaatacggg gagggtatgc tatgaagatg gacaaggaga
    29101 ccttgtcgaa acatgacggc agggaaggcc acaaagcgta tgttgccgtc gaaggcaggg
    29161 tctatgatgt tacgggaaac cgcctttgga aaaacggcgt gcatatgaac cgtcatcagg
    29221 ccgggaccga tctgacggaa gccattgcag cctcgccgca tggaaaggac gttttgtcca
    29281 aacttcagga catggggcag atgagccctg aaaaagtggc caagccgccg ttcattcccc
    29341 tgtggatgca tcattggctt gaggcctatc cattttttaa acggcatccg catccgatgg
    29401 ttgttcattt ccccatggcc atttttatca tcgctcctct ttttcttgcc tggtattacc
    29461 tgatcaatcc ggtacccgga ctgccggctg ctgtttttta tctctacatt ctgggtacgc
    29521 tgtcgctgcc ggcagccatt gcgacaggcc tgctggcctg gctggttaat tattcgggac
    29581 agccgaaccc tctgattata cgcaaaacga tattttccct gttgctgctg ctttgcgacg
    29641 gaatcgtgat ggcggcgctc atcatcagcc ccgcaatcct gcaggatccg tccggaagcg
    29701 agtggatgat accggttctg atctttctgg ggctcccgct ggcatccatt atcggcgagc
    29761 acggcggcag gctggttttc ccggtgttga agaacaaata gcggcggacg tcggaagatg
    29821 ccattgcggg gcggtttata acgtatcgtt tcgtctcttt gatggtgacc gatcaaacca
    29881 tgaacgaatg attttttcac cgcaacctgc cggcggtttg ccgggaacag cccttttctt
    29941 tttattgtgt tattgactga taaggtttaa tatagcggtg tccctgttac aactccttcg
    30001 gatatttgcg cccctggttt tccggcaagg tacgttagtg aagtccataa caaacccaca
    30061 tgcacaagga taataaggag gcctgccatg ccatcaaaaa aactcaaaga gtttctgaac
    30121 agccaatcgg tgaaatacgt catcattgcc cattccacgg catacacggc acaggaaatt
    30181 gcgcagtctg cgcacattcc gggaacggag ctggccaaga ccgttatcgt ttacatagac
    30241 ggcaagatgg ccatggcggt tctgccggcc agctattcca tcgattttga tgatctccgg
    30301 aaggaaacgg gcgcaaagaa agtggaactt gcgtctgaag cgcaatttaa ggatatgttt
    30361 ccggattgtg aaatcggttt cgtaccgccc ttcggtaatc tttacggaat ggacgtttat
    30421 gtggatgagc atcttacccg caataaggaa attgccttca atgccggcgg tcatctggaa
    30481 ctgtacagaa tgtcttatcg ggacttcgaa gagctcgtaa aaccgaaagt gctgaaattc
    30541 acctgaacgg acatgatgcc attcgccgtc tcttccaaac ctccgtttat taaataatga
    30601 gagaaagtgt tatggagttg aacatcatcc gcaaggccat ggcgcataaa acaataagtc
    30661 ttcacgtcct cctgacggtt tttttagtga cgaattacag cgcaatcacc ggcgcggcag
    30721 agacaggaaa aaagagtctc ctgccggatc ggtctgtcat cacgcttccc gctcccgtgt
    30781 atcaaggcag gatgtcggtg gaagaagctt tgagaaaacg tgaatcggta agacaatttt
    30841 cacaaagccc attaacggag caggaattgg cccaacttct ctgggcggcg cagggaaaga
    30901 cgcgtgactg ggcagctagg accgcaccgt cggcgggcgc cctgtttccc ctggaggttt
    30961 atgttgtcat ccgggaaggc gtcttccgtt attcatccgg agaccatcgg attttgcgtg
    31021 tcgtcaagac agacgttcgc cggcagttgg ctgcggcggc gctcggtcag gacatcatcc
    31081 ggcaggcgcc tgcggttttt gttattgccg gtgtctataa acgaaccgca gaaaaatact
    31141 caaaccgcgc caaaagatat gtggagatcg aagcgggtca tgccggtcag aatcttctgc
    31201 tgcaggccgt gtcgctggga ttgggcgctg ttccggttgg cgcttttcag gacgcacacg
    31261 tcaaacaggc gctacatctg cccgtcaatc atgagccgtt gtatctgatc cccgtgggca
    31321 gaaaacgttt tgtccccccg tagaaaaaaa agtttcccgg aattttatat cgaagatttt
    31381 cttgacagaa aaacgcggct gccttaaact tttatgacat aaaaaggttt cgctgaaatt
    31441 ttcccaaaat caaatttgtc gttacaatgc ttttgaactg taaatagtca ggaaggtgta
    31501 ttttatgggc gatttcgaca agagaaaata catccggtat cccgcggagc cgaacgaact
    31561 ggttacggtc tactatatgg acgagaaaac cacaaagacc ggacaacgcg tggggctagg
    31621 cgagaatgaa agttttaagg ggtgctgtgt cgtttttgtc ggcgacgtgg atttcaaaca
    31681 gggacaggaa gtgttttgcg aatgtggaac gcttcccaaa acaaaagccc aaatcgtctg
    31741 ggttaaaaag ctggaaaacc agattaccaa agtcggaatc tctttccggg aataactccc
    31801 gactctaaag gtcaacgatt tcatcttcat gacggaacgg tttttatgat ccgcgctgaa
    31861 aaacggacac agcgcgaaaa tgaaagcgac gtttcaaaaa caaagcgtgt tgaaacccat
    31921 ggattgagcc gtctcccttg ccgacatgaa aaatgacata aatcggcagc cgcgacaagc
    31981 gttgctgctg gagaaataaa ataacctgaa cgttatggct gaattctttg agacctttat
    32041 cccggctcct gtcttcgacg caaagaaaaa gggaggaaag tcagttagcg gttttttttc
    32101 ggcagaaacg catggaaccc gtttttgatg atttcactga agtgcagagt tagatcatcg
    32161 agcgacattt ttatccggtt gttaaaccag tcctgataca gaccgacaaa tccggaagca
    32221 tacaaattga cgcaaaccat gaactgttct ttgtttttaa tggcagacat ttttttttca
    32281 tcgcttacta ttttatctaa aaaaattccc ttcaatttat tcaaaaaaat tatcgatcgc
    32341 tgggagttga tcagaattct gtaaaattcc aaatcttttt ccaggaaacg ggcaatattc
    32401 atcaaaaatg gcagagggtt ttcgatcagt ctgctgtttt taaattcatc aagaaactcc
    32461 agtaatgccg agatgatttc attgccaatc tgctctccga cggcatttac attgctgtaa
    32521 tgggcataaa aagtgccgcg attaatatcc gctgttgtga caatatccat gacggtgatt
    32581 ttatcgatat ccttttcctt gatcaatgag acatacgcct gcttgatcaa ttttctggat
    32641 cgaatagcgc tgcggtactc tgctttttcg tggattgtct ttccgctcat ttttgatctc
    32701 ctgtaaaaaa tctgcctaaa gtacgacaaa tttcatcttt gtgttcaatt ttatacaata
    32761 tcttcaaata ttgagtattg actgaaacac aagtgcctta tattgtactt atgttcaaaa
    32821 atcaacatgt gtttgacatt tgaacaaaat aattggccgg agcgcggccc tcgaaaagca
    32881 ggcgggcaac atgaaagggg agaaacaatc ctttcaacag aacctgaaaa gcaaactatg
    32941 acacgatcgg caatgaatgg atcatggttg cttaaaataa aaaatccgga ggcaatcgaa
    33001 cgacacaaaa aagcccctcc agcggttaaa accgcttctt cttattcctg tcctaagtaa
    33061 cccctgggtt acgacggcag gttcctttaa caccatccct ccagcaatgg agggatggtg
    33121 ccttttttta acgtcagacc gttttaaaac atcgcatttt tagaaaacaa aaaaagtatt
    33181 ttcgaaaaaa agagtgtcgg gaaaaaacga tgaacggctt taactccggt ttcaggaaac
    33241 tgacagccga tagtgcattt ctatgatggt tcgaccgtat tggtgaatgc cggccctgct
    33301 caattagttt ttttattttt gaagggatgg cggtttcccg tcgggaagga acgatttggc
    33361 atggcagcgt gaaaaatccc gaacggacat tttacatttg ctctccgggt ccaggaaatt
    33421 atgccgggcc gttactcaga tcgcaatacg acgtcttcaa gggcgcattc cttacgggcg
    33481 catggataaa tgattcgcct gaaaaaaatc cgttcctgcc gacggctcaa atattatttt
    33541 gcttaatcct ttttcgcttt tttcagatcg atatgacagt agccgcgggg atttttcttc
    33601 aggtagtcct gatggtaatc ttccgccgga tagaaatctt ttgcaggctg aatttccgtg
    33661 acaatttttc tggtatatct gccggatcgg gcaagttttt ctgcagaggc tcttgccgct
    33721 ttttcctgtt gcggattggt ataaaatatc gccgaccgat attgggtgcc cacatcgttg
    33781 ccctgcctgt taaggctggt ggggtcgtgc aattcccaga agtgttgcag cagccgttca
    33841 taagttacga ttgtcggatc ataggtaacg cggacagctt cggcatgtcc ggttttgccg
    33901 ctgcagacct gttcataaga gggggcttta acggttccgc cggtgtaacc cgattcgctg
    33961 ttgacgacgc cttttacccg gctgaaatac tcctccacgc cccagaaaca gccggcggca
    34021 aaaatggccg tttcggtcga tttgccggat ggagcagcaa ataacgacaa gtactcaccg
    34081 tagccttccg tctccatttt ctctcgggga ataaatttta gtgatgcgga gttgatacaa
    34141 tatcgcctgc cggtgggggc gggcccgtcg tcaaaaacat gcccgagatg aatgtccgct
    34201 tgcgtacttc tgacttccgt gcgtagcatg gagtggctga aatcggtctt tctccagacg
    34261 gcgttgtcct tgatgggttt tgtaaaactg ggccagccgg ttttggaatc gaatttatcc
    34321 ttcgaactga acaggggaac gccggaaaca atatcgacat agatgccttc ctgatgattg
    34381 ttccagtatt cgttttgaaa aggcggttct gtggcgttgc cgaaggcgac atcgcgctgg
    34441 agcggcgtca ttcttttttc ggcatcctgt cccgaaattt ttttatccat gattctccct
    34501 ccctgcaccc gttcggacgt aacttgcgcc attaaatata cccatatgaa tccaaaaagc
    34561 atgacaagaa taaagacggg cgtgtatttg tttttcatca tttcaccttg aaaagaaatt
    34621 taatcattgt ccgacaagag agcaagatca ttttaatcaa cggatgaatt taagcatttt
    34681 tttcaggtgt tgaggcgatg gcggctcttt ggggttatct tgctgtccag gaaaaatttc
    34741 cggagtttgt ctccgtaacg gcttttgcga taaattttta accagcggtt gacgatggcg
    34801 cccgcgtatg tcctgacaaa cagatttctc catattcttt tgatatcttt ccaggcgctg
    34861 atcagcggaa cggcaaaaat cgtccgcgga ctccaggcgc cgtcgggccg tttaccggca
    34921 cgttgtcttt ccgccaaatt acggataaac cggcggatgt aaaattcgac gaattgaatg
    34981 ggaaatccga ttgtggcaat ataggtccag ttcagccagt tgccgtaatt caaattccgt
    35041 ttgataaatc ctccgagata tttttttttc atcagcaggc cgggcatgcc ctgcagttca
    35101 aatgcgtcca tactgtcggg ccggggatcg tagcagagga aaagcccgtc gtatttatcc
    35161 cagcccacta ttttcagggg cagaagacgg ccttcccgtt ccagtcttgt tctgagatcc
    35221 gaacccggaa gcggaaccgc attcaatacc tgaatggtat cgataccgga ttttttaaaa
    35281 aaatctttgt agattttcgc ccgttgtttg acggtgaaag aagatggccc ctgtgcatcg
    35341 tcgttgagcg gatagccgaa aatgaacatg ccgtgcagat aaaaaacatt ggagagtttt
    35401 cgggaacggg caatcaactt ctccacggtt acgcctttac gcatggcccg gagttcttca
    35461 ttgatcgggc tttcgtaccc gatcgccagc atgctgacac cggcaaaatg catggcctcg
    35521 atgagggcgt cattttccgc cacttccgaa cgcacctgaa ccaccaaatc gatttttttc
    35581 aaaaaatttc gtttataatg gccgatggct ttgcacagag caatggcctc gtccggatgc
    35641 tgtgcaaaat tatcatcggt gaaaaagaat ttagtgtatc ccatatcggc atatcggatc
    35701 agttgcctca gcgccgtatc gatggacgca gccttgtatt ttccgtattg tttgttcacg
    35761 acgcagaatt cgcaattcca gttgcagccg cggccccggt tgatggggat atagctccat
    35821 cttttttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ctcttgtatt
    35941 gccgggcgat ggcgaacatg cgggggatcg aattactcat gccgccgtac aacagggcca
    36001 ggtgtgccgg ttgttgctgc tgtaaaaaga aatgatccgg cagaccattc aaatcctccg
    36061 gcccgcggta attattctca tcgatcgcgt agatattcct gatgcgggga tgacggacga
    36121 tctgactcat gacggatatg atcccgacgg gcggcatttt aatccgggcg taaatattct
    36181 gttgggaatg tttcgggtac tgcggaatga tgccgatcac ggttaaatct ctttccatga
    36241 tgaaaccctc cctgtaaatc tttcatcaga aaaggacggt tcgaaaataa acgttgggct
    36301 ggaaaatacg gctctttgga tgataaaaat aatccattca ccgcaaaaag tcaatattgg
    36361 cgatggacaa gctaaagcaa gaaggatgcc ttgccgcaaa ccatcaataa aaaaagcacc
    36421 ccggctgatt tttcaaacaa cacgggatgc tctatttatc tggtttctac cagggcgttg
    36481 tgcgcctggc agggggaagc gtcgctcatt agatggctct gacgttcgtg gcttcctgac
    36541 cttttttgcc ctgcgcaacg tcaaaactta cgcgctggcc ttcatttagg gtcttgaaac
    36601 cttcaccctg aatcgagctg aaatgtacga atacatcgcc gccttcttcc ttttcgatga
    36661 agccgaagcc tttgctttcg ttaaaccatt tgactttacc ttctgacata gttctcatcc
    36721 tcctttcata aattttcaaa agtcgaatca gcacgatgac agaactaaaa aagccaccaa
    36781 gactctaaag tacccggcgg ctaacctatg tgcttcaaaa tgtctaaatt caactctttc
    36841 cttcaaatct caatcttgtg gatctgaagt tggcgcgacc ttacggtatc gcggaggaat
    36901 agtcaagctt tatttacaaa acaaattctt ttctcaagtt atagtgtgcg ggaagagtcg
    36961 atttcatcat ataatgcagg acgttacagc gtttatgctc cgggcaaacg attgctcatt
    37021 tcaggccgga tgtgaatgag attctgctcc gcgaggaata attccacttc cttaaagacc
    37081 ataccggaaa tgttgccgcg aacaatgccg tgcagatcat gatcgatcca ggcaaaacgt
    37141 tttttgtttg tgcccagacg tctgaatatt tcaggaccgc tttgcggcgc gactttcgga
    37201 tcgtttttcg cctgaatcac cagtgccgga atgcgaatgt ccggaagcga ccggttgact
    37261 tttttcatca gctttttcac ctgaacaaat gcgcttaccg gacagagcag atagttgatg
    37321 tggggattat ccgcgtcgtt tttgatgtag tctctggcaa gggtgttaac gccccgggaa
    37381 tggcagaatc ggttgaacgc gttgagaact tccgcaaacc tcgacgaaaa gcttttgaat
    37441 ttcagcggtg cgctgacggc gatcacggct tcaaaatcat ggggttttaa aatgacggat
    37501 tgcagtgcaa ggcccgcccc ggtggaaaaa ccggccacca gaattttgtc gcagtatttt
    37561 ttcaaaatgg catggccgcg ctccgccgaa tccagccagt cttcatagcg gcgtgtcgaa
    37621 agatctttgc aagatgttcc atggccggcg agcctcggtg cataaacggt cagacctttt
    37681 tggtgcagaa actccgccca ctccctgact tcttccggcg cggccatcag gccgtgaatc
    37741 agaagcacgc cggtatcgga tccgggcgcg tgcagaaaaa acggcatgcc gatctgttga
    37801 ggctttgttt cgccggcaga ataatgctgt tgatactctt tgaggaataa atccgcttcg
    37861 gctgccagaa ggcaggccgc ggccgtgtca gggggtgtac attgcattag tgtatcgctc
    37921 aggcaccgag ctgcgcataa acgccgtaaa tcatggcgta atgcagagct ccaccaatca
    37981 gaaccaggat gtggaaaagt tcatgaaaac tgaaaatgcc cggaatcaat ttcggttttt
    38041 tgatggcata aataatgccg cctgccgtaa aggcgccggc gccggtaaac agcagaaccg
    38101 tctgggaaat agacatactg gccaggacct gtttaataat gaagagtccg gaccatccca
    38161 tgaataaata gagcatcgcg taaagtgtcc gcggcgcgcg cgggaaaaaa atctgagaga
    38221 ttacgccaac cccgacaagg ctccattgaa aagcaatcat cgcccatcgt tggttgccct
    38281 ccaggcaaaa atagcagacc ggagtaaatg tgcccgcaat catgaaaaag atcgccagac
    38341 gatccattct tcgccagaag gacagctcgt tttccttctt tttgaaggcg tggtatagcg
    38401 aactggctga aaaaagaaaa acaacggaaa tcccgtaaat cagagcagtg accagaccgg
    38461 atgccgagaa actggcttcg atggccagat acacggtgcc gataacggcc gcgatcattc
    38521 cggccaaatg tgaataaaaa ttaaacaact cctgggtttt gacattcata aaatcctatc
    38581 ctaaaattcg atttttaacc tgttgaatct cattcacaga tttgcataaa acacaagcaa
    38641 ttaatgttgc gcttcactaa ggattcttgt tggccgttcg cattcgacgc ctttcagagg
    38701 aaaaggagtc gcatggatat cggctctcag cggacgggaa gggcgcttga ttgccggaat
    38761 caaacggggt tcctgcttcc gattaaattt ttgacaaaaa gcatttcttc ggatatccct
    38821 gacgcgggga caaaccaatc gtctgtccga aaatacattc atgaccaaac aggaaacaat
    38881 caacagcgcc gtgtcttttt tcaacgaggg atacgcctgc tctcaatccg tcttgctggc
    38941 ttttgccggc cggttcggcc tggatgaaaa gcaagcgaag cggattgcct ccaccttcgg
    39001 cggcggcatg ggcagactgc gggaaaaatg cggcgctgtt accggcggct tcatggtgct
    39061 gggactggca tacggcaacg ccgatcccaa cgatatggaa accaaactga ccgcctatcg
    39121 gaaagtgcgg gggttgaacc ggaagattga agatcttcac ggcagcagtt gttgttccga
    39181 gttgctgaaa aaacatgcta caaccgccga cgtaaccgaa agaaagcatc atcagatcat
    39241 ctgtcaaaaa attgtcggcg gtgtcgccgg aatattgtac gacatgctgg aagaagaacc
    39301 gccgcgcgca agataagtct gaccggcaga tgccaaggga ttcgtcagcc ttcaggtttt
    39361 aaacatcaaa gacggaaatg ccggtgaata cctggcacag atgctcatca cgtcgatcag
    39421 tatttttccc cctccttgtc cagcatgggc acgaaaagga ccgggatgat cgcctgctgt
    39481 tcaaggcgtc cattgcgttt gaccaaaaca tacaactcct gcgctttgac atttcccacg
    39541 ggaatgacca ttctgccgcc ttccttgagt tgtttcgcaa gaggcgcagg cactttggtg
    39601 ggcgcgcagg tgaccagaat ggcatcaaaa ggcgcggcct ccggccatcc ctgatagccg
    39661 tcgccggtcc tgacatggac attttggtaa tgtttcccga gaatcttctc tgcgcgtctg
    39721 gccagcgttt caataatttc gatggtataa acactgtggc agagttcggc cagaattgcg
    39781 gcctgatagc cggaaccggt gccgatttct aaaaccttca tatccgcttg agggttgatc
    39841 acttctgtca tccaagcgac aatgtatggc tgagagatgg tctgtccttc cccgatggga
    39901 agagcggaat cggcgtaagc cattttgcgc agggcttcag ggacaaattg gtgacgttcc
    39961 accttgctca tcgcccgcag cacctgcgga ttttttatgc ctctggcttt gagctgctcc
    40021 tcaaccattt tttttctcaa gacgtcatca ttttgttcca caccggcccg gcagttcagc
    40081 gcgcagaagg ataaacataa cgtggcaatg aagataacca ttctgaaatg tgcggacaga
    40141 cgcaaatgtg cagccggtac agcggatagc tgcccgtctt gccttgtcgg ccggccgtaa
    40201 aagccgggcg aggatgcgtt gccggttttt tcgttcatga tcgttccagc cgtcaatatt
    40261 tttcatttca gaaacaggaa taagcatctc atttttacca tatcctggct tacaaatcag
    40321 cttttttaaa aaaaatgagt aaaaaaatcc ctgccggggg gacgtcagaa aaaatggaaa
    40381 aaagaataga aaaatgaacg tataactggt aaaaatgcat tcgaaagaaa gttcatgcat
    40441 tattgaaaca ttaaacgatt ttcagaggag gcagaaaatg aaaggttatt ttaaaatcat
    40501 ctcaatgttt gtcggcatgt gtgttctgct gtgcgcatca tgggcggatg ccggtgttta
    40561 ttataaaaca gtgcaacagc cgccggcggc ttccgaaaag atcaaggccg gcatggattt
    40621 cagcgttctg cccatcgatt acagcgccat caatccaaag gatctgggct atacaaataa
    40681 cgcggaatgg gatgccgata aaagtgcagt tcccaaggca tttgccgatg ctttcccggt
    40741 tctgttgaag gaagccaata tcaccaacaa aaagatatcc gtggtgagcg gtggcgacaa
    40801 gatcacccag ggtgtgattg cgcaagtggc cgtgaccaaa attaatctga actggaacgc
    40861 ctggacgaac cggcctgatg aatatgtctg caaaataacg tttaccgatg ccgccgacgg
    40921 gcagaaatta ttctcgggcg aggtgaatgt cagcacgttt tccggaaatc cctttaccag
    40981 cgcctggggc agtttcagcg gaagaatgca aagcgcagcc tacaacatgg cctgggttct
    41041 gacgaaaatc atgacgcagg gcaggataga gccggccgaa cattgattcg atttttcgac
    41101 tgaaagcaag catttttgaa aacatcaacg gcggatgcat gtgttgtttt gcatgctccg
    41161 ctcttgctat atttcagtaa atattatagg tccagagcgt attttttatg gaactgaagg
    41221 agtttgaata ttcccatgac agacgagaca gcctcaaccc cgaccgattt tatccgcgac
    41281 atcatcaatg acgatctgaa aacgaacaaa aacaacggga aagttgcgac ccgttttccg
    41341 ccggaaccga acggctatat tcatatcggt catgccaaat ccgtatgcct caatttcggc
    41401 atcgccaggg attataaggg aacctgcaat cttcggctgg acgacaccga tccggcggga
    41461 gaatcgatgg aatatgttca gtcgatcatc aacgatatcc gatggctggg gttcgactgg
    41521 gaagaccggc tgttttacgc ctccgattat ttcgaaaaac tttatcagtt tgcggttgaa
    41581 ctcatcaaat ccggcaatgc ctatgtctgt tctcttaatc ctgatgaagt cagagaacaa
    41641 cgcggaacgt tcacggaacc gggcaaggaa agcccttacc gaaaccgctc ggttgaagaa
    41701 aatctcgatt tgtttcaacg gatgcgcgcc ggcgaattcg ccgacggcgc ccataccctg
    41761 cgcgccaaaa tcgatatggc ctcgcccaat atcgtgatgc gcgatcccgt gatctaccgc
    41821 atcaagcgcg aaccgcatta ccgcaccggc acgcaatggg tcatttatcc gatgtacgac
    41881 tttgcgcatt gcctttccga tgcgctggaa gggattgttt actccatctg caccctggaa
    41941 tttgaaaaca accgtccgct ctatgactgg attgtggaga aactcattac cggtctgcgt
    42001 ccgcgtcaga tcgaatttgc ccgcctcaat ttgagttata cggtactgag taaacgaagg
    42061 ctgatcgaac tggtggagaa aaagtatgtt cgggggtggg acgatccgcg catgcccacc
    42121 gtgtcgggaa tgcgccgccg cggttatacg gcggaagcca tccgcaattt ttgcgccggg
    42181 atcggcgtgg ccaaaaacga taatctgatc gatgtggcgc ttctggaaca ctgtgtgcga
    42241 gaggacctga acgagcacgc tccccgggcg atgtgtattc tgcgcccgct gcgcgttgtc
    42301 atcgacaatt atccggacaa tcaagtggaa cagatcgcct gcgccaatca tccgcaaaaa
    42361 ccggacatgg gcacacgaaa cgttcccttt tccaaagtcc tctacattga gcgcgacgac
    42421 ttcatggaag atccgccgaa aaaatatcac cgtctcggtc ccggacgcga agtgcgtttg
    42481 agaaacgcct acgttataaa atttgaaagc atgatgaaag acgaaaaaac cggcgacgtc
    42541 accgagctgc actgcagcta tgatccggcg acactgaacg gcccgccggc cgatggacgc
    42601 aaggttccgg gcgtcattca ctgggtatcg gccggacacg ctgttcccgc cgaagtccgg
    42661 ctttacgaca ggttgttcag tattgaaaat cccggaagcg aagaggattt tctgaaatat
    42721 ctgaatccgg attcgctgga gacgctgaca acctgccggg cagagcaaaa cctgaaaaat
    42781 gccgcagcgg gcagccggtt tcagtttgaa agactgggat atttttgcgt ggatgcaaag
    42841 gattcgacat ccgaaaggct cgtgttcaac cgggttgtcc cgctgagaga ttcctgggcg
    42901 aagattgccg gcaaataatg aaaacgtttt gaaaacagcc cgttttgccg catgaagtag
    42961 atagcgatgt aatttctgaa tcgagagatc cgcaccatga agacaagcgg caagacggca
    43021 cttctgttta cgctctacct ttcacagggt cttcctttcg gctttcaggc cacggctttg
    43081 ccggttcttc tccgcgacaa aggcatttcg ctcacgacga tcggttttgc cggagcgctg
    43141 gccctgccct ggtttttcaa acccctctgg gcgccgttga tcgaccgctt ctggtcggca
    43201 agaataggac ggcgccgttc gtggattatt cccctgcaga ccatgatggt tttgacgatg
    43261 ttttcgtccg tcttcattgc cgcatccggt tcaattcctc tgctgctgtt gtgtattttt
    43321 ctcatgaatc tttgcgccgc cacacaggat attgccgttg acggcttggc ggtggacatt
    43381 ttgcaacgtg atgaactcgg tcccggcaat gccgcacagg tcgccggtta caaggcgggc
    43441 atggttctgg gcggcggcat actggtctgg ttgagcgccc gttccggatg ggagagcctg
    43501 tttctgggca tggcggcgct ggcggtcctg ccgctgattg tcattttgtg ccatcgggag
    43561 gtggaaccgg atggaagcat tgtcagcaag ccgccgaaat tttccgattt tatcagcgcg
    43621 atgcgcaacg ccctgcagtc tcccaaggcc ggttattttc ttttgttcat cgccacgtac
    43681 aagatcggtg aatcgatgat cgacatgatg ttcaaacctt ttctcgtaga caacggtttt
    43741 accgtccagc aaatcggatt gtgggtcggc acctggggaa tggcgtcatc gctgatcggg
    43801 tcggttgccg gtggatggct tgcagccagg atttccattg tacgcgccct tttcctcgcg
    43861 gcgtcattga ggttgatccc tctaatcggt gagctctggc tgtccacgtt tgtgcccacc
    43921 gcggcgcagg tggtgagcgt taccgttgcg gagcattttt ttggcgggat gctgaccacg
    43981 gcgcttttcg cgttcatgat gtcgcgtgtc gacaagaaaa tcggcgccac gcattatacc
    44041 gtgctggcgg ccattgaagt ttttggaaaa tttccgggga ctgttttctc gggctgtctt
    44101 gcggaaaata tcggttatac gggacttttt tctttcggag tggtgctgtc gcttctggtg
    44161 ctgctgctct atttcccgct gcgggaatca acgtcgccgc cgaaaatgaa tatataaaag
    44221 gaaattgatg aaaaaaatag tcatgatccc gaaaatgttt ttgaaattat atttttaccg
    44281 tcttcggaca gatagtttat aaattgaaaa aaataatcaa tgatcaagga tgtttacgcc
    44341 ggtccaggaa aaaccgtttt ccaaatcgaa ccgttcgggg cagaaattgc ttgacatgta
    44401 gagtcaaatt ttttatttat ataggcaatg aagaggttgt tttgttgtag tttccaagaa
    44461 atgtgaaaca cggttaagtc aaaaaatttt tttaaaagga gacgatttat gaaaaagatt
    44521 ttatcagtga ttgtcggatt tatgatgttg atgatgatac ccgggatttc aattggcagc
    44581 gatgcatcac ccgccaaaaa agccaaagca gcgaaattta aagcagcggc agtaaacaaa
    44641 ccgtatgtaa gcgtacaggg cggtgtggcc tttttaacgg acagcgatgt ggacgcaggc
    44701 atcagaagca ccgccatgga ttttgatccg ggttatgcac tgggtgttgc agccggttac
    44761 aagatgggca aatacagagt ggaaggcgag atcggctatc agaagaatga agttgacgaa
    44821 gctctttccg gccggagaac ttcaggcgat atgaaggctt attccttcct ggtaaacggc
    44881 tattatgaca ttgccaccaa gaccagattc acaccttacc tgaccgcagg tatcggtgtt
    44941 gcaaaagtcg acggcgattt taaccgcatt gctcatgtcg atgacaccgc cttcgcatat
    45001 caggtcggcg ccggtgttgc ttatgccgtc aatagagctc ttgctgttga tttgaagtat
    45061 cgttacatgg atacggttga tgattttggt ttcggaaaca ccaatcccga attctcaaca
    45121 cataatgtat tcctgggctt gagatactgc ttctaatcga ttttcagaaa tcgatccagt
    45181 gaagccagag gtctgtaaaa gacctctggc ttttgttttt tagttatctg tagaaatccc
    45241 aattatcaat atctgtgttt tgatccggat accggaggat tgtctgttta atgccttgaa
    45301 acatcggtac agttttggta tgctccgcgg cacaaaattg ttctatgaac aattttgcgt
    45361 ttttattttc atataaaggg cttgtcttat gaattattcc caacgtgttt tttgcggcga
    45421 actttcgcag gaggatgtcg gccggaccgt tcagctttgc ggctgggtgg acgcctaccg
    45481 cgatcacggc ggcctgctgt ttattcatct gcgcgaccgc agcggcattg tccagattgt
    45541 tttcagtccg gatgtcacac ccgccaatgt ctgtcagact gcggcaacgc tgcgtcccga
    45601 atactgtatt acggtaacgg gcatggccag aaaaagaatg gaaggcacgg aaaatccaaa
    45661 catcgaaacg ggaaagctgg aagtttttgt cgacgggatg acgattcttt ccgaatccga
    45721 tccgttgccg tttgtcatca ccgacaaggc gatggtggcg ggcggggaaa ccgcgggcgc
    45781 ggaaaatgtt tccgaggatt tgcgcatgca gtaccggtat ctggatatcc gccgtccggc
    45841 gatgcagaat aatctgctgc tgaggcatcg tatttttcag ggcgtgcggc agtttctcga
    45901 ttcgcgcggg tttgtggagg tggaaacgcc cgtgctgacc gcaagcacgc ccgaaggggc
    45961 gcgcgattat ctcgtcccca gccgtgtcca tccgcagaat ttttacgcgc tgccccagtc
    46021 gccgcagctc ttcaaacaac tgctgatgat cggcggcctg gagcgatact ttcaattggc
    46081 gcgctgtttc cgcgatgaag atctgcggcc gaaccgtcag ccggaattca cgcaactcga
    46141 tatcgaagcg tcatttatcg acgaggaatt tctctacgaa ctgattgaag agctgacggt
    46201 caaaatgttt gccgtcggcg gaattgaatt aaaacgtccg tttgcgcgca tgagttacgc
    46261 cgaggcgatg gacacgaccg gttccgaccg tcccgatctg cggttcggcc tgcgttttgt
    46321 ggatgtgacg gacgccttca agagcacaaa ttattccatc ttccgccaga ttctgcagcg
    46381 cggcggctgc atcaagggca tcaacatcaa gggccaatcc gaaaaactca gcaaaaatat
    46441 tttgcagaat gaatacgcca aagacatcgc gccgtcgttc ggcgccaagg gcatgacctg
    46501 gatgcgcgcc gaaaacggca agctggaatc caatatcgtg cagtttttca gcgaggcgga
    46561 actggacgaa ttgaagcgcc gcttcgatgt tcatgacggc gatgtgttga tcatggtggc
    46621 cgatccctcc tacagcattg tcacctccgc gctggggcag ttgcgtctgc atctggccga
    46681 ccgtctggga ctgattccgg aaaacagttt ttatccggtc tggattaccg aatttccgtt
    46741 gtttgaaccg acggacgagg gaggggtgac ttccagccac catccgttta cagcgccgga
    46801 ccgcacggat ttcgatccga aaaacatcga agaactttta tccctgaaat cgcgcgccta
    46861 cgacctggtc atgaacggcg aggaactggg gggcggcagt atccgtatca acaatcgcga
    46921 cctccagcgc aagattttcg cggcgctggg attgacggaa gaggacatga aggaaaagtt
    46981 cgggtttttc ctgcgggcgt ttgacttcgg cgcgccgccg cacggcggac tggccctggg
    47041 catggaccgt accgtgtcga tgattctgca gacggcgtcg atccgcgaag tcatcgcgtt
    47101 tccgaaaaac cgcagcgcgg cctgtccgct gaccggcgcg ccctccgcgg tgaaacgcga
    47161 gcaactggcg gagctgggac ttttgaatct gggtgaaaag gatgtgctgc cgggagaagc
    47221 tcataaagaa aacctgctgg atcgcgtctc gtgggtgtcg cgtctgggca ttgccgaggc
    47281 ggagcgtccg gtgatggagg aaattcttgc gcagtccgaa acgctggccg cgcaggttca
    47341 cgatctggcc ggcgctgaag agccgatccg gtcggttgcg cccgcggcgg gcaaaacacg
    47401 tccgggagcc gaggccatac ggtcgccgct ggccgagacg ggacaattgc tgaaaagcgc
    47461 gcccgcagtc aagggaaatt atttcaaagt tgccaatatt atcgaataga gggagacatc
    47521 ctgatggatt ccattttttt ctatcaaaat cctgaacagt cggcaggctc aggcaaaggg
    47581 gctttgcaaa aactgaaagt gactgttcaa cctaatatct ttgtgaaggg ctggccgacg
    47641 gaagcgggat cggcagcgct ggccggtttt aaagccctgg aagacgccgc tgtggtggag
    47701 cggctttccg gagcgggcgc cgttctgatc ggttccacgg ccatgagcga gttcggtttc
    47761 ggactcaaag gaagccaagc cggttgtgcg ctggggaaaa aatccgccga tgtggaactg
    47821 atgctggaca tggccggaga agcgcgtctg gccgcagccc gcgaaggcgt ttgcggcttc
    47881 aaaccgagct atggtttgat ttcgcgcatc ggtcttgtcg gcctgattcc ctccatggaa
    47941 acctgcggaa ttttagctcg tgatataaaa tccgttcggg atattcttga aacgatagcc
    48001 ggtccggacg aacgcgattt ttctcttccc gatgaaaaga tgccggattt ttcagcgcaa
    48061 gacatcgagc cggcaaaaac gacgctaggc atcattccag aagttgtgaa cggattgcct
    48121 tcagatcagc gcgaagcctt tgccaaaata tgcgacgcgc taaaaaaaga tggatttcaa
    48181 atcaaggaat tatcctttcc tgatttctcg catttcctgc tggttcatca gattgtcggc
    48241 tcggtggaag cctcttcctg cgcgggacgc tacgattccg tgcgttacgg ccagcgggtg
    48301 ccgggcgcca aaaactggaa tgagatgtat ctgcaatcgc gcggtgcggc cttcggaacg
    48361 cttttgaaaa gctacctgtt tcagggagcg tttttccagt ttgaaaaata cgatgcctac
    48421 gacgatgcct gcaggcttcg cgcgcgtctg gtcaaagaga tgcaaaccgt tgccgggcag
    48481 gcggatgctc tgatattgcc gaccgtcaac cgcgcggcat ccgacaaaac cggatcgctg
    48541 gccgatctgt acgcgcagtt tgcctatacc gcgtttgcca atgtgaccgg ccggcccgcg
    48601 ctgcatatac ccggcgcaaa cggcgcgggt gtgcagttgt ccgcaaaacg gcttgatgac
    48661 gggcgtctgc tttcgttagg ccagtatctg ttgagccatc gtcagggagg taagtcataa
    48721 tggaatttga agcggtcatc ggattggaaa ttcatgtcga actcaactgc gcaaccaaat
    48781 tgttctgcga ttgtcccaac aatcccggcg acgaacccaa cgccaacacg tgtccgacct
    48841 gcctgtggtt tccgggcgcc gttccccgtt taagccgggc cgcgctggaa aaggcggcgc
    48901 ttctgtgcct ggggctcaac gccgaactcc agcccgaaag cgcgtttgac caaaaggttt
    48961 attattatcc cgatctgccc aagggttatc agctttcgca ggcgcatctt ccgctgtcgc
    49021 gcggcggctg gatcgacatt gtcgatgaag acggatcgac caaacggctg cgcattcatc
    49081 acattcacat ggaggaggat gtcgccaaac tggtgcacga aatggaaggc cgcctgccga
    49141 tcagtctggt ggatttcaac cgggcgggcg c
//
LOCUS       DAONQZ010000012        49087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_766_length_49087_cov_16.898842, whole genome shotgun sequence.
ACCESSION   DAONQZ010000012 DAONQZ010000000
VERSION     DAONQZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 49087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49087
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_49087_cov_16.898842"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLR13_03720"
     rRNA            complement(<1..266)
                     /locus_tag="PLR13_03720"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            848..>2187
                     /gene="glpK"
                     /locus_tag="PLR13_03725"
     CDS             848..>2187
                     /gene="glpK"
                     /locus_tag="PLR13_03725"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQC18472.1"
                     /translation="MKKSYILSLDQGTTSSRAILYNHKGNIVGTAQKEFTQIYPKAGW
                     VEHDPMEIWGSQSGVVSELLATTAIAAEEIAAIGITNQRETTVVWEKRTGKPVYNAIV
                     WQCRRTADICDELKARGLEKYIRENTGLVLDAYFSGTKVKWILDHVEGARQKAQDGEL
                     LFGTVDTWLIWNLTRGKVHVTDYSNASRTMLFNIKKLKWDQKILKELDIPASMLPDVK
                     PSSMVYGHTDSRLFGAEIAIAGDAGDQQAALFGQACYTPGMVKNTYGTGCFMLMLTGE
                     KLVHSQHGLLTTIAWGINNKVEYALEGSIFVAGAAIQWLRDGMKLIYDAKDSEYFATR
                     VEDSLGVYVVPAFVGLGAPYWDMYARGAILGLTRGTTRNHIIRATLESIAYQTRDVLE
                     LMRNECGLDLCELRVDGGACANNFLMQFQSDILGVPVERPEIIETTAMGAAYLAG"
     assembly_gap    2188..2287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2288..3745
                     /locus_tag="PLR13_03730"
     CDS             <2288..3745
                     /locus_tag="PLR13_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC18473.1"
                     /translation="CGIARDLALRGIPHCLVEKGDFAAGATGACHGLLHSGGRYAVTD
                     PVAARECYEENLILRRIGRKCVEQTGGLFVRLPGDSKRFRELFLRGCEDVGIPAEVIS
                     STLAIDLVPNLNPDIEEAIRVPDCSIDPFRLCLLNIRTSELRGGMIFTRHKVTEIIKS
                     HGRCTGVKAVDQTTGEEKEIHAKIIVNATGAWADIVATLAGSEVPLTLVKGSLVITNH
                     RLTNMVINRLRQPSDGDIIVPNEAVCLAGTTSLAIENPDNLLIEASEVDTIISEAGRT
                     IPHFNKTRIIRAFSGVRPLLKSKKADSHEITRGFQIIDHQNGLLSIVGGKLSTFRLMA
                     EKTVDTIMTIFHLKKACETAEIPLDGQEELSGYPLSKRLADMTNIVCECELVTRQNVE
                     KIIQQTATRNVGDISHRTRLGMGPCQGGFCTFRALGIMNDMSIISPEQSMEMLRGFLQ
                     RRFKGIRYALWGDQLREEQLVEYIYLGILSMEKPQ"
     gene            3742..4950
                     /locus_tag="PLR13_03735"
     CDS             3742..4950
                     /locus_tag="PLR13_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit B"
                     /protein_id="HQC18474.1"
                     /translation="MSVTEYDAVIIGAGVAGLTAGTLLAERGLKVALVTKGEPTACLS
                     TGCVDVCSLSKNPLSGIRSLPPEHPYNLVEEKTVKQSLEKFLADMKDAGLPYSGSADA
                     NRLILSALGTSKISCLIPSTMTAAPQENHDGSVHIITFKGLKDFYPGYIVSRRRSTTF
                     SVYDAGVSTTMSIAARFEEKDFFERFIAWMTQRGIHEDGIAFPAVLGMESAADIVDTI
                     SERTGKPVFEIPTIPPSMPGRRLFNALKKTFRNKGGTIYWSWPVAGMEKTGNIVEAVT
                     TPSEGRPNSINARAFILCTGSFVSGGLTATREGILENIFNLPVYVPGPRDTWFDNDYF
                     SLNHNIGKAGIRADASMRPLDAPWKNIFVCGSILADTQILKNGCGHGLALATAHVAAQ
                     SCAEYLLDEI"
     gene            4940..6112
                     /locus_tag="PLR13_03740"
     CDS             4940..6112
                     /locus_tag="PLR13_03740"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HQC18475.1"
                     /translation="MKFEHCIRCTICVENCPVFRVNPEFPGPKQAGPDAQRFRSERDK
                     AQDGWVLLCSQCKRCEMACPCGVEPAQIILKAQQRYGNEHPQTPAHLLFANNYYLSAL
                     GSFTAPLANVVASTQTGKKIFQKMGISTYLPFPKFSFRTMRREKKRLNKGRKKVAFFY
                     GCFINFYRPDIGRKIIRLLASMNVDVVLPPQWCCGLPALGNGNLTLARYFAKKNAESL
                     SNYIDAGYDVIYTCTSCGLCLLHDYPGILEIPQGRKIAENSYDVHEYIIKLINEGYVK
                     PDFREVRRSIAYHIPCHLRALKIGYPAAKLMSLIPGLEYEILDDACCGLSGSYGFKKE
                     NALTAIQIGNRAVSLISKTGMDNIVSDCGSCRMQLAGLSGMPALDPAEIIFDSLGR"
     gene            6211..6909
                     /locus_tag="PLR13_03745"
     CDS             6211..6909
                     /locus_tag="PLR13_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQC18476.1"
                     /translation="MYKILIVDDEKDIVDLVSYNLEKEGFSTVKALDGESALQIIRAQ
                     KPDLIILDLMLPKMNGLDVCKTVRRNPETSGLPIIMLTAKSDEVDKVTGLEVGADDYV
                     TKPFSVKELTARVRSVLRRLKENELYAGKEKFSHAGLKINYESCLVLVNGKKVTLSPT
                     ELKLLFFLSRHPGRVYSRNQIIDHVWGDDTFITDRAVDVHIRRLRSQIEQDIENPKYI
                     LTVRGFGYKFADLK"
     gene            7022..7843
                     /locus_tag="PLR13_03750"
     CDS             7022..7843
                     /locus_tag="PLR13_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQC18477.1"
                     /translation="MFSILKKAALTIAVVALMSSVALAGSSIVIKGSTTVLPITQSTL
                     EAFMKKHPDVQMSLSGGGSGEGIKALIDKTAHIATSSREIKKEEIALAKTKNVNPVAH
                     VVAHDAIVPVVHPRNKVKNLSVDQLSQIYQGKITNWKEVGGDDLKIVVISRDSSSGTF
                     ESWDHFVMKKTKVTPRAQMLASNGAIVTAIAKNRYAIGYLGIGYLDKSVTPLQVNGIT
                     ATIQTALSKQYPFSRELYMYTDGNATGDVAKYLAFVKSAEGQKIVQKEGFVPLTK"
     gene            7884..8318
                     /locus_tag="PLR13_03755"
     CDS             7884..8318
                     /locus_tag="PLR13_03755"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQC18478.1"
                     /translation="MQNDSMKKNVLFLCTHNAVRSQIAEAFLNHLYGDRYSAFSAGSD
                     PTQIDPLVVTVMKETGMDLSANQSKSLSVFPDMHFDCVITVCDQVQESCPHFPRGDRH
                     IHKRFSDPSKFNGNAEDITHEYRRVRDEIKAWVEKEFHQAIK"
     gene            8325..9212
                     /gene="pstC"
                     /locus_tag="PLR13_03760"
     CDS             8325..9212
                     /gene="pstC"
                     /locus_tag="PLR13_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQC18479.1"
                     /translation="MTRQIKEIIIKYIFFIFSLVSVLVLGLIVFSLFREGIPLFGKIS
                     IKDFLFGMEWYPTYDPPSFGIFPLIVGSLIVTILSTFIAVPLGVLAAIYISEIAPQNI
                     KITLKSVIELLAGLPSVVLGFFGMVIVAPWMQETFDLPTGLNIVNASLILALMAIPTI
                     SSISEDALYAVPREFKEASYALGATKFETIARVILPAALSGISTAVMLGMARAIGETM
                     VVLMVAGGAAAIPESLFDSVRPMPASIAAEMGETPFRSLHYQALFAIGIVLFCLTLAF
                     NLIADYVSHKFKQVGSGTL"
     gene            9224..10102
                     /gene="pstA"
                     /locus_tag="PLR13_03765"
     CDS             9224..10102
                     /gene="pstA"
                     /locus_tag="PLR13_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQC18480.1"
                     /translation="MQILQTHAMKWRYFKQGLFFSCVRLSALMITLALGGIILYILIN
                     GISVISWDFITKPPTDAMTKGGIMPAILGTIYLTLGAIAIGLPLGIASAIYLTEYARQ
                     GRVVRLIKVGINCLAGVPSVVFGLFGLGFFVIFLKFGSSILAGSLTLGFLILPTIIGA
                     SEEALKAVPQTFREASLSLGVSKWQTIYRIVLPNALPGILTGSILGIGRAAGETAPIM
                     FTAAAFFTAKLPSSIFDEVMALPYHIYVLATAGTNIEATRPIQYGTVLVLVAVVLGID
                     LIAIIIRGYMRKNKRW"
     gene            10105..10860
                     /gene="pstB"
                     /locus_tag="PLR13_03770"
     CDS             10105..10860
                     /gene="pstB"
                     /locus_tag="PLR13_03770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQC18481.1"
                     /translation="MDSRIIQLNNVNFYYGESRALTDISMTFVKNKVTALIGPSGCGK
                     STLLRLLNRMNDLIDGTKVEGEILFESKNIYAPDVDPVEIRRKIGMVFQKPNPFPKTI
                     YNNIAYGPKLTGIAVDDMAALVEQSLKQAVLWDEVKDILNKSAMNLSGGQQQRLCIAR
                     ALAMKPDVLLMDEPTSALDPISTAKIEELIEELKKDYTIIIVTHNMQQAARISDETAF
                     FYLGNLVEYNKTEKIFTNPDVKQTEDYITGRFG"
     gene            10882..11565
                     /gene="phoU"
                     /locus_tag="PLR13_03775"
     CDS             10882..11565
                     /gene="phoU"
                     /locus_tag="PLR13_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQC18482.1"
                     /translation="MEERRHTSSHYEMELQDIKNNLIYLGALTEKAIHSAMTSLTDRD
                     TDLAKKVIANDYEIDKMDKDIEEKCIKMLALRQPTAIDLRFITTAIKITGHLERIGDM
                     AVNIAEKAIQLNEEPKLKPYIDLPNMSSIVEKMVAKALDALIKRDLQIADDVRQIEQE
                     VDDLNEQIFRELLTFMMEDSKSIHRALLIMHVSKNLERIADHAQGIADMVTYMITGEN
                     VRHLSRDEK"
     gene            11577..13316
                     /locus_tag="PLR13_03780"
     CDS             11577..13316
                     /locus_tag="PLR13_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18483.1"
                     /translation="MKKNLFYKIFFSYLVIICLSFFLLDIFVRDEVRKVLTSQIETEL
                     LSYAQLIDQTSLEKASGHLKQVAQISQSRITFMDARGRVFADSEKDIALLESHFNRPE
                     VQEARLRGTGKSVRYSDSLGIDMLYMAVAVKDAQKIKGYVRLARPLHHVQNVIDKVYQ
                     YILLTLFVVALIALGIALFFSYRLYEPIKIMEQFTEKLRRGDPVGSIILDTSDETKTL
                     ADNINYLVEELREKINIANEEKNKLMTAFTSMNEGVLILDEQGVIEFSSPVLSNMLAI
                     QYDDVVGKTLMEAFRNIDLQKAFQEFKEKRISVSREIILGHADKVVLNVSLSAVHGRP
                     DEEKTMIVFHDVTRLKKLEQVRMDFVANVTHEIKTPLTAIIGYLETIQSGAITNQEET
                     KKFIDIILKQAERLNRLVEDLLTISNIETKQTVLNFESVSLRKAIDTMLSLIEAKAEL
                     KNISVRNNVSDNFPPITANRDRLTQIFVNILDNAVKFTPNGGEISINASESGSYTVVS
                     IQDSGIGVPKDDISRLGERFYRVDKSRSRDLGGTGLGLSIVKHLMIAHGGRMEIESQP
                     GKGTKVSLYFPVK"
     gene            13591..14979
                     /locus_tag="PLR13_03785"
     CDS             13591..14979
                     /locus_tag="PLR13_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQC18484.1"
                     /translation="MTNAKRAEVHAIAMETVKAAEKVPEKYFVVDTSALEYGMDVFEK
                     LRQGGRNCVLIPYATWEELDSHKTGNDFKADIARNVIRNVRNLFRAKDHSVRFFQYDF
                     NLVKDADLKVDKNDHCIIATALNAKKKLGKAKVILVTQDAALEALALNFGLDVQSIKM
                     HQTDIKQHHTTRATINLQRSWISDKTFPLKLPEAKLAAQLEQVLYNEGIICYCDLDGV
                     WGPAFVAVRKGDYFRIIDRDIHLLGIKPRSNNGDVNWGQFIAMDLLRDATVPLVTLTG
                     KAGTAKTFLALLAAFEQASHYRQILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIY
                     DNINAIKELVDERMKEKINDWLANGKIEMMDLDKIRGRSIQKCYVIVDEIQNLPPSHV
                     KAIATRGAEGTKMVFTGDFSYDQIDVPWLDERSNGLSVLNAKMAGSRFFGSVHLDQAI
                     RSELTKEILERW"
     gene            15408..15653
                     /locus_tag="PLR13_03790"
     CDS             15408..15653
                     /locus_tag="PLR13_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQC18485.1"
                     /translation="MADSKSDFFTGLIVGGLIGVALGILFAPKSGKESREDIARKADE
                     LMAKAQEGYEKAAGKYSEMTGVESFRDYKTADNEDLA"
     gene            15898..17169
                     /locus_tag="PLR13_03795"
     CDS             15898..17169
                     /locus_tag="PLR13_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HQC18486.1"
                     /translation="MDGETALIFYYLKGNLNSYNALAGALEKYPDIKNIDVYFLSSEK
                     ELLQKISAISKKHRETIIGISFCTPQFLKIKDLINRVKKLAGETVFLAGGPHPAGAPL
                     ETLKLGFDFVISGEGEETLTELLSKINHDENNSDIKGLSYLDKNGEYVFTGRREAADI
                     NTFYPISHYFSKYGPIEITRGCPYACRYCQVSQLFGTTPRHRSIENILDCVKIMKRQD
                     STYFRFISPNAFSYGSEDGKRINNEKLENLLSDIRRLVGKSGKIFFGSFPSEVRPEHV
                     NAHTVKMVKKYANNDNLIIGAQSGSQRMLDCAGREYDVATIYNAVDVTRQAGLKAKVD
                     FIFGLPGETDEDIASTLNVIQDLSRSGAKIHAHTFMPLPRTAFENETAGKIGPALKKE
                     LNRLCANGIIYGQWQQQEKIAAGIAAKQDNA"
     gene            complement(17180..18868)
                     /locus_tag="PLR13_03800"
     CDS             complement(17180..18868)
                     /locus_tag="PLR13_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18487.1"
                     /translation="MDWKLKAVYKSILENETGYVKKIWGKDNTVCLVYPNHYRIGMAN
                     LGFQTVYQLLNAQPSFLCERAFLSVSGNEARCVSGTAGIFSLENQKALTEFDILAFSI
                     SFENDYPNILKIMDDAGMPLRSADRSEKYPLILGGGIAPTLNPEPLADFFDLFVLGEA
                     EDILPVFCRFFEASRRLGQRREQFLKDAQKQLHHVYVPGLYDVHYSPERKITSVVPRE
                     AGLPEKIKVEPVNDINAFRTQEVISASCTEMEDMFLVEVNRGCAHGCRFCAASFIYRP
                     VRFRGIEEMIGAIHDGLTRKNKIGLVGTAVSDHPDIPAVCRYIIAHNGRAGLGSLRID
                     RINEEMVGLIKANGIETVALAPEAGSQKMRDILKKKISEEQIMYATALLAEHGIANIR
                     LYFMIGLPREEESDIDAIIGLTKKIQHHVRLRFDGKKQFRRLTLSINQFIPKPATPFQ
                     WVPLLDVHIAGKRIKKIKEAFRKDKSVRVIHDVPKWNYVQALLALGDRRVGEILLAVN
                     RLDGNWGRGLKEVNVNPDFYVYREKSFDEILPWDIIDLGISRKALINENQKALK"
     gene            complement(18935..20092)
                     /gene="ftsZ"
                     /locus_tag="PLR13_03805"
     CDS             complement(18935..20092)
                     /gene="ftsZ"
                     /locus_tag="PLR13_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQC18488.1"
                     /translation="MFELTEVCSENLAKIKVIGVGGGGGNAVNTMIASCLQGVDFINA
                     NTDAQALGVSSAPVKIQIGSQITKGLGAGSDPEIGKQSALESRDEIRPYLEGADMIFI
                     TAGMGGGTGTGAAPVIAEIARECSILTIAVVTKPFQFEGKKRNMQAEEGIEQLREKVD
                     TLIVVPNQRLLSLGGRNLSLLEAFKKADDILYQAVKGISDLILVPGLINLDFADVRSV
                     MSNMGMAIMGTGIASGENRAVEAAQRAISSPLLEDNTIQGAHGILLNITGGSDMSLYE
                     VNEASSLIQEEADEDANIIFGTVIDQNMEDEIRITVIATGFDDFARKKNDSVGNVTRF
                     RGVGGREDLATPTFIRRERKLNSGEIAVVGLGDETENMDIQIPTFLRRQAD"
     gene            complement(20108..21325)
                     /gene="ftsA"
                     /locus_tag="PLR13_03810"
     CDS             complement(20108..21325)
                     /gene="ftsA"
                     /locus_tag="PLR13_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQC18489.1"
                     /translation="MGKRSNVLVGLDIGTTKTTAIVGDVTETGIDIIGIGTSQTKEMR
                     KGTVVNIDTMVESIKTAVEEAEHMSGCRINSAFVGISGSHIKGQNSLSIVSIKGREVD
                     EKDVQRVIEASEAIAIPVGRKILHILPQSYVIDGQEGIKDPIGMSGVRLEAKVHIVTG
                     AIAAIQDIEKSVNRVGLDIDGIVLEQLAAGEAVLSSDEKELGVVLVDIGGSNTSVTIF
                     SESSIKHTAILPVGGNYLTSDISTGLRTPLSEAEKIKIKHGCAMTSMIPKDETIRVPS
                     LGGREDREVSRQILGRIVEPRMEEILNLAYNEVVKFGFEDLLAAGVVLTGGTSLLEGI
                     SELAEKIFDMPARKGIPIGVGGLNDIVNSPAHAIGVGLIIYGRNQNILRGTSTGMLGS
                     ITRTVKKMFSEFF"
     gene            complement(21358..22197)
                     /locus_tag="PLR13_03815"
     CDS             complement(21358..22197)
                     /locus_tag="PLR13_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQC18490.1"
                     /translation="MLKKTGIQTKQNRIRRRFTSLSGDFFTAMTLLSMAVFLSVLFIY
                     VYNVMMSSPYFQIKEISVRGLKELTEKDILASANIKPQQNLLAVNKNSIIRRVCSNHW
                     VETVYVGKELPGKLVLDVRERIPLALVKRGGDFYLMDVKGHVFKRLGGSDEVDLPVIT
                     GAATGEATRSPLLLSALGLIQRISKSPAYAHLGTISEIHIDSVFGLALVSDNGLYLKL
                     GTDDFENKLRKLKAILTDLENRGMKTGFLCVDLSDHSKVTVKRKYVPEKTQDGDQNKN
                     YLI"
     gene            complement(22191..23120)
                     /gene="murB"
                     /locus_tag="PLR13_03820"
     CDS             complement(22191..23120)
                     /gene="murB"
                     /locus_tag="PLR13_03820"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQC18491.1"
                     /translation="MTSHCEIRGILQEIDAGKVLYDEPMSLHTSLAVGGKADALVVVE
                     HENQLARVIRKFRMEGINYFTAGNLTNVIVKDGGYRGAIILMTGMKEIQCKPVGGHEG
                     YILAQTGASLAKVVSRSVVEALSGVEFCSGIPGSVGGAVWMNAGAYGREMKDVIESVT
                     VLDTEGSKKTMIREAISFGYRTSSFPSGTIILGARFRLKKGDRATMKARISEILQWRQ
                     AKHPLEYPSAGSVFKNPPGHPAGKIIEDMGLKGEKAGGAEISSKHANFIVNKGGATAA
                     DVLALIRLVQSRAREEKNIDLETEVVVIGEDRC"
     gene            complement(23120..24532)
                     /gene="murC"
                     /locus_tag="PLR13_03825"
     CDS             complement(23120..24532)
                     /gene="murC"
                     /locus_tag="PLR13_03825"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQC18492.1"
                     /translation="MKKDKEKSLMQRKIKCIHFVGIGGIGMSGIAEVLVNLGYNVGGS
                     DVQPSETTARLQKLGAKIVIGHAAENVGNADVVVTSTAVKEHNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTILAEGNLDPTMVIGGKLASIGSNARL
                     GDGEIIVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIAEIKEAFLTFANIVPFY
                     GCTVLCNDNIHVREILPAIKRRMITYGIDTPADYSAEDIKFTKRKTCYSLVHKGEKLG
                     KIELIVPGLFNVYNSLAAIAVARELNLDLATIKEGLKSFSGVQRRLEIKGRVGEVTVL
                     DDYGHHPTEIKATLAAMRQIWKDRLIVVFQPHRYTRTRALFEEFTKAFDEVDILILND
                     IYPASEEPIPGITAAALCEAIRKAGHKHVEYIPGAEATVEYLLKTVHQKDTVATIGAG
                     SIYKIGETFITQLVARGKNT"
     gene            complement(24562..25647)
                     /gene="murG"
                     /locus_tag="PLR13_03830"
     CDS             complement(24562..25647)
                     /gene="murG"
                     /locus_tag="PLR13_03830"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQC18493.1"
                     /translation="MRIVIAGGGTGGHLFPGIAIAEEFIKRNAQTKVVFIGTKRGIES
                     RLLSRLGYDLETIDIEGIKGRGIRALVKSVFQIPKSLWQARGILKQFSPDMVVGVGGY
                     ASGPAVLAAYLMGIPTSIAEQNAIPGVTNKILGNVAGKIFVTYPRTQQCFPRRKVVIS
                     GNPVRASFAAQSFRTKEDRTHRQLLIFGGSQGAEAINRGVIDMLPRLSGMKDRIRIVH
                     QTGARQMEEVKKAYAQSGLQAVATDFIMDMAEAYAQADLIICRAGATSLAEITVAGKA
                     AVLIPYPWAANDHQLKNALAMSDAGAAVVIPEKELSGESLFNAIDGILRDENRRLLME
                     ENSLRMGKAEAAATIVDECMKLTDAKH"
     gene            complement(25702..26811)
                     /gene="ftsW"
                     /locus_tag="PLR13_03835"
     CDS             complement(25702..26811)
                     /gene="ftsW"
                     /locus_tag="PLR13_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQC18494.1"
                     /translation="METAYKKEDNYPDFVLLLVTLLLAVVGTAMIYSSSSIIALEKFN
                     DGQYFLKKQLVFVFLGFTIMVAMTKVPYTHLRKIAYPGMLLCLLLLSLLFVPGIGMRR
                     GGATRWLNLGGFSFQVTEMVKIMIVIFLSHLLTRKAFQITNFTKGVLVPLAATSVVMM
                     LILLEPDFGTTAIIATIMLMMLCIAGAQIKHLALLIGAFIPVGIALILYKGYRLTRLT
                     AFLDPWKDADNTGFQIIQSLISFGSGGTFGVGIGDGMQKLFYLPEPHTDFILSIIAEE
                     SGFIGVAMVITMFALFAYRGFRIALNAPDLFGSLLASGLTMVICMEAFINIAGVMGLI
                     PLKGLVLPFLSYGGTAFIMSMTAVGILLNISTQTS"
     gene            complement(26812..>28068)
                     /gene="murD"
                     /locus_tag="PLR13_03840"
     CDS             complement(26812..>28068)
                     /gene="murD"
                     /locus_tag="PLR13_03840"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQC18495.1"
                     /translation="IVVTDEKSPDHWDGGFKQIAGEPWLEVGEYNSVDVLNDADMVIP
                     SPGIPPGNPLLAEALKKNIAVVSEIELAYRFIRVPIIAVTGTNGKTTTTTLLGEILQK
                     SGKKVFVGGNIGNPLIEYADKNQDADFIVAEISSFQLQWIDQFRPFIAVLLNITSDHV
                     NYHGSFAEYRRIKARIFANQTGKDFAILNAEDDEQKKIERNIRARVAGFSSKHVLPSG
                     IFVKEENIVLSLPGMQEEKYPLSMINIPGLHNVENVMAAILAARFCGCDPSDTIRAVE
                     NFRGLPHRIEFVEEKNSVKFFDDSKGTNVGSVLRALETFTQPVILLLGGRDKDGDFET
                     LQPVLAAKAKKIVLFGEARERIASLIGQTVPVIKKTKLQEAVEVAYQEAVPGDVVLLS
                     PGCASFDEFANYKERGNFFRKVVENL"
     assembly_gap    28069..28168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28169..28972)
                     /gene="mraY"
                     /locus_tag="PLR13_03845"
     CDS             complement(<28169..28972)
                     /gene="mraY"
                     /locus_tag="PLR13_03845"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQC18496.1"
                     /translation="MIYHLLYPLHTTISFFNVFRYITFRTIFAAITALLICLIFGKWM
                     IRKLQDMQIGQQIRDDGPQSHLVKKGTPTMGGMLIIMAVVVSTLLWTNLADVYVWLIL
                     MVTLGFGLIGFIDDYSKLAGKSSRGVSGKTRLAAEIIIALFVSVILYVKPGFNAQVTI
                     PFFKTVLPDLGWGYVLLSTFIIVGAANAVNLTDGLDGLAIGPVVICFATYILFAYFAG
                     NIKTSNYLQIVHVSGAGELAVFAGAIVGAGLGFLWFNAYPAEVFMGDVGS"
     gene            complement(28989..30401)
                     /gene="murF"
                     /locus_tag="PLR13_03850"
     CDS             complement(28989..30401)
                     /gene="murF"
                     /locus_tag="PLR13_03850"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQC18497.1"
                     /translation="MLTSTSGTMMSGLPETVFRGVSTDSRSIKKGNLFVALKGDHFDG
                     HDYAESVFQGGAAGILAQDDEKIRRMKVDDTFAVIKVADTLFALGEMAHDWRKKFSIP
                     VIGLTGSSGKTTAKEMIASILGLKKNILKTEGNLNNLIGLPQTIFRLTDQHDMMVLEM
                     GTNTRGEIRRLTRIAEPDIGLITNVGPAHLEGFGSLQVVGEEKGDLFLNMPSSGIIVV
                     NLDDEAILTAAETWKGRKITFSMSPNADVTVFDIERNGAKGVRFNLAIRGNTQKVEMK
                     ITGLHHINNAMAAAAVAVAAGMDLKNIAEGLSAFRPFHGRMEIIKLRNGAYLLDDSYN
                     ANPASVREALMTLKDLKGNHHAYVFLGDMLELGAQADEMHRKIGMLIATIGVKTLFLQ
                     GDYAGMTAAGATEGGMASDSILFLSDKENGTDFLKKCLKKGDWILVKGSRRMNMDRIA
                     AQICEDFGRDKVSEENKTDY"
     gene            complement(30446..31969)
                     /locus_tag="PLR13_03855"
     CDS             complement(30446..31969)
                     /locus_tag="PLR13_03855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQC18498.1"
                     /translation="MKLTRLTEGLDMINMSGEASGDVSGVCFSADQCVPESLFVAIPG
                     LKRDGHDFIEEAISRGARFIVHEKVLTFPPFVRAIKVSDSRRALGILAKNYFGDPSAA
                     LTVIAVMGTNGKTTISYLLESILKEAGCKCGVLGTVNYRYNDKVYDAPNTTPESYEMQ
                     KILRAMADDGVTHVVAEVSSHAVDLKRVDDCDFALGIFTNLTRDHLDYHVTMENYFQA
                     KKRFFAEILPQSRKGYAQKMIINADDDWGRRILKDVPLPALTYGIENDCAVRSLNCKL
                     SLNGIEADIRLAGNNVLIQSPLIGKFNLYNILAAAGAAFILHIPVPVIKAGIENLSHV
                     PGRLESVPSAGGVHVFVDYAHTDDALRRVLQNLSELKKNRIITVFGCGGNRDKGKRPL
                     MGDAATRYSDLTIITSDNPRLENPLDIIADIETGIDRKDVKKSDPEMLKINGRARSYA
                     VIPERRKAIEAAIRAARRDDIVLIAGKGHEDYQILGTEKISFDDRVVARQILETKSY"
     gene            complement(31977..33953)
                     /locus_tag="PLR13_03860"
     CDS             complement(31977..33953)
                     /locus_tag="PLR13_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQC18499.1"
                     /translation="MAIESKKRMKFRIIMIFMFFFVLFVALISRAFQLQVLSGQKLKK
                     LATRQHTTVLQLQPERGIIYDRNGEKLAISIMADSVCADPSKVIDPAKASKQIAYILG
                     LDSQAVYKKLSAPRNFCWLARRISPEQTAAVVKADVEGVFLVKEPKRFYPNGELAAHL
                     LGFVGLDASGLEGLEKKYNDSLVGSPEKLEWARDAKGKKLFLRVEKTQTAKNENTNLV
                     LTIDNRIQYLVETHLKEAVIEKGAKGGLAIVMDPKTGEILALANQIGFNPNNIQGLSP
                     ERWRNRAITDYYDPGSTFKPFLVAAALEEKVIREQDRFFCENGNYRIADRVIHEANRK
                     RYGTLTVREILKYSSNIGSAKIAQKMGKEKFYNYIQKFGFGSKTGIDLPGESSGLVRP
                     VRDWVKVDTSMIAFGQGISVTAIQLISAVSAIANNGVLMKPYIVRGIADQDNRLVKLY
                     TPTVVRQVISPRVAKSVTNMLMDVVGAEDGTGKNAKIVNVAVAGKTGTSQKFDAARGV
                     YSSEKVRTSFMGFFPAEDPQVAILVVLDEPQKDKWGGVASAPVFKNIAEQILNCFKTD
                     IRETPVFEKEKTDKVQLVSAEQTLLPEIVENSDGPSMPNFMGLTIREALKKAKTESIE
                     LKISGNGWAVRQYPEPHAPLGHERVCSVVFELTD"
     gene            complement(33955..34302)
                     /locus_tag="PLR13_03865"
     CDS             complement(33955..34302)
                     /locus_tag="PLR13_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQC18500.1"
                     /translation="MAPSAQSADARVVPHLGEVEGASVGIKYPTFIVLAVVLMLVFVA
                     YVGSHIRMTELEYNIAAEISTQEKILEEQKKLKLEYAMLKSPQRIENLVRDRLQMSYP
                     DNEQVIVIRKSGG"
     gene            complement(34304..35239)
                     /gene="rsmH"
                     /locus_tag="PLR13_03870"
     CDS             complement(34304..35239)
                     /gene="rsmH"
                     /locus_tag="PLR13_03870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQC18501.1"
                     /translation="MTAEFYHQPVMVKEVIDSLIVSRAGVYVDGTVGGAGHSYAILKQ
                     TDALLIGIDCDDEALRFAEKRLQEFGSRKILLKENFVNLDHILKSLNIEKVDGVLLDL
                     GVSSRQLDASERGFSFSQQAPLDMRMDRSMTLSAYDIVNSFAQNELEKIIRFYGEEKM
                     ASRIARAISKKRQESPIATTTQLAAIVASCMPAGTRWRKIHPATRTFQAIRIAVNHEL
                     DNIKPAMHAAVDVLKTGGRLCMISFHSLEDRIVKNEIRSLEGRCVCPPDSPFCVCQRD
                     AKLKNLTKKAIMPSEEEIASNPRARSAKLRVAGRL"
     gene            complement(35243..35689)
                     /gene="mraZ"
                     /locus_tag="PLR13_03875"
     CDS             complement(35243..35689)
                     /gene="mraZ"
                     /locus_tag="PLR13_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQC18502.1"
                     /translation="MSVFRGQYHHTIDEKGRIIFPSKFREIFAEEYDNRMVITNWDQY
                     LMVFPYDEWLVIEEKVAHQSKLRKEVRSFQRFFMSGAIDCSIDGQGRVLIPPSLREYA
                     QLEKDIVLAGMLKNIEIWSKDRFEERMKQAVGEIDKCADFIADLGI"
     gene            complement(35894..37075)
                     /gene="mltG"
                     /locus_tag="PLR13_03880"
     CDS             complement(35894..37075)
                     /gene="mltG"
                     /locus_tag="PLR13_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQC18503.1"
                     /translation="MKNPDKKNILKKSNEENNAVDQMTSRAIAWKSKFYEIFIFLKKI
                     FSGRRWIRRTVLYSVAVILIFCIMLLDYAFSPIDVRDKTVVIDIPTGSSFMEATELLQ
                     KEGLITNRFFFYSLAAIKRASRNIRAGEYEINTLTNPITMINKLMHGDIKVYEVVIPE
                     DLSFRDIAVILDKHKLINKEIFFELARDQEFLKSLNIEAESIEGYLFPDTYHFNRSMN
                     TRRIMKKMVDTFWQKMTPSMIRRANQMGLSVHKLVTMASIIGKESGSALEKPSVAAVF
                     YNRLKRGMPLQSDPTAVYDMDDFEGKIFRSHLKRNSPYNTYRIKGLPPGPIANPGLDS
                     LKATLSPSSVDYLYFVSKKDGTHHFSSSLVEHNQAVNRYINLKDEQNQLLEYDIPVEE
                     Y"
     gene            complement(37089..37514)
                     /gene="ruvX"
                     /locus_tag="PLR13_03885"
     CDS             complement(37089..37514)
                     /gene="ruvX"
                     /locus_tag="PLR13_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQC18504.1"
                     /translation="MRILGLDYGEKRIGAAICDELGLTAQGLSTIIRKTKKHDGEILE
                     RLIRDFNVEKIVIGYPLKLDGSEGIQCEKVNRFAALLEKTFGLQVIRWPETLSTKEAE
                     EILINSGIRWGKRKKMVDKLAACLILQSYLDSVAPSLKK"
     gene            37681..39594
                     /gene="selB"
                     /locus_tag="PLR13_03890"
     CDS             37681..39594
                     /gene="selB"
                     /locus_tag="PLR13_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HQC18505.1"
                     /translation="MKHFVLGTAGHVDHGKTALIKALTGIDTDRLKEEKQRGITIELG
                     FASLKLPSGLTLGIVDVPGHEKFVKHMVSGAAGIDLVLMVIAADEGIMPQTREHLHIC
                     SLLGISRGIVALTKIDLVEKDWLNLVESEITDFLRGTFLESAPVIPVSAIKQEGLSEL
                     LQALDTIIAEITARTDDGIFRLPVDRIFTMKGFGTIVTGTLVSDHLKTGEDVQILPQN
                     ISARIRGIQVHNRPEEEAWAGQRTAVNLQGIEKSAIVRGNVLVRPHTVWPTQRLDIAV
                     DYLSANTKSLKNRALVRLHTGTNEIISRIILLDTDELAPGQQGYAQLILSEKDVVVAG
                     DHFVLRSYSPVTTIGGGVIIDPLPQKHKRFNRGAIEELMLLQKGTLPEKITVILDRTG
                     FKGTPLQQLAFRLGMKSRKIREVLDDLFSKKEAFLLDSEDTTVISARFFNRLEEMIIY
                     NLSGYHKKNPLKEGIPKEELKGSLANDISPKLFNMVLNTLNKKETIVSNKDNVRLAKH
                     QVELAGDLDALRQNIIGIYKTAGLMPPSLTDVINKFIDRKNKAQNIVSLMLKEGELIK
                     INEELCFSGEILSKLRNDYKAMLIKDGKATPASFKELTGLSRKYIIPLMEYFDMNKLT
                     VRVGDHRVLREKT"
     gene            39591..40364
                     /gene="fdhD"
                     /locus_tag="PLR13_03895"
     CDS             39591..40364
                     /gene="fdhD"
                     /locus_tag="PLR13_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQC18506.1"
                     /translation="MNDFQKIIKTIRCFSGSYSAVSGSRVKVIQEVMITVRVNKTPLA
                     NIACAGIHLEELAAGFLRSEKIITRRQDIENISLAKNRVDVLLKNKNFFPDVSVKNIA
                     SSGARGRSNVALLTPLNVPAGLSINTALALKLMDDLLNGSAMHNETGGTHCSALAFRK
                     KIAALREDIGRHNTIDMLGGYALLNKLNLHRAIILTTGRISSEIVYKIWNMGVPFIIS
                     HSAPTANAVTLAKQANITLIGYVRKGRMSIYSHERRVII"
     gene            40361..41350
                     /locus_tag="PLR13_03900"
     CDS             40361..41350
                     /locus_tag="PLR13_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC18507.1"
                     /translation="MNHKNVYLKDIKQGDKVASSFLVTEKNMAFSQKGSPYLTVRLKD
                     KTGEMECKVWENALEFDNVFKKGDVIFIEGRAASYKNSLQISILNIKNIPRQDIDPAD
                     YLPAVKTDVKTMFAELLAFAEKVNHRHLKALLNAFLQEEETAARFQRAPAAKGFHHTY
                     LGGLLEHTLSVVRLLDKVADHYSILDRDMLITGGILHDIGKIYEFSFESIIDYSDEGR
                     LIGHIVMGVEMIDQKIAAINGFPPQLAMEMRHIILSHHGEFEFGSPKRPKTLEALVVH
                     YIDDLDAKFNAFQTFMNESSSPDSDWTVYHRFLERFLYKKKNAAFDIPEDPKI"
     gene            41479..43488
                     /locus_tag="PLR13_03905"
     CDS             41479..43488
                     /locus_tag="PLR13_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC18508.1"
                     /translation="MAYRTDTLCGIFHNQALRYGDQVELLRAKFDQNGNPSAQWHSRT
                     WKETREEAIGLAKGLMVLGLKKSDRIVIFSESRPRWIIADQAIQACCAIGVPLYPTLT
                     VEELGYMTSDSGSKIVIASTQGRGEMALKAKAQGVPIEKIIVMCSWEGAKPEGVYTFD
                     EVIDLGKGKISDEEIEKNINSVTPEDVTSIIYTSGTTGKQKGAILTQKNWVSNMLQAS
                     NSTLMRKQRELGLHLTFLVHLPMCHVFGRTSDYHVGALQQSGILVFAESFEKIPKNIL
                     EVRPNVLISIPRLWEKIYDTTRSIVSRQDKRVQSIFNWATRLGEKYCEAMATATRLGF
                     LDLLQFALANILVFNKIKKTAGLDHVVLGISGGGKLSKEVCVFIRALGIQLAEGYGLT
                     ETSPVINFNEPEFATSFDISKASWFQKKMLDLTVDLMVTKQAKGLSPYTNPLRSLSLS
                     LAYNTLAHKLQIKPGTVGRPALWTEEKIAEDGEILVKGPQVFQGYWNLPDATKEAFTD
                     DGWFKTGDIGEFDKDGFLTITDRKKELFVTSGGKNIAPHPIELALTAKPYIDQVCLIG
                     DARKYISALVVPDFQELHRFSKHNGIPACSDKDLLANEQVKELIQKQVDEVNEALPRY
                     EQIKYYKLLDKPFSVETGELTPTLKLKRRIVFEKFKDDIESMYKD"
     gene            complement(43580..44380)
                     /locus_tag="PLR13_03910"
     CDS             complement(43580..44380)
                     /locus_tag="PLR13_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQC18509.1"
                     /translation="MNYKFIELEVKDHIATVTLAREKSLNAVSLELASEITDVFRKLS
                     AMDDVRVVILKSRARIFCAGLDLKDAAGGFTGTTRAPWDMIKKSQPLFDCCNVIEECP
                     KPVIAAVHGQCIGAGLHIISACDIRLCTQDATFCLKEPKIGLCADMGALQRLPLIVGQ
                     GYAREIAFTVAFYNAQQVEKMGLVNHVYADQAALYEAAREMARQITENAPLAIECTKE
                     VLNFSRGTSVSEGMSLAIQKNMFLLATDDLKEAFVAFMEKRQPDFKGR"
     gene            complement(44410..45069)
                     /gene="nadD"
                     /locus_tag="PLR13_03915"
     CDS             complement(44410..45069)
                     /gene="nadD"
                     /locus_tag="PLR13_03915"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQC18510.1"
                     /translation="MRWGLFGGTFDPIHFGHLRGAEEMLEIFNLNRIIFVPSSRPPHK
                     LEADITAFNHREQMIRLAIEDNVNFSFSEVENLRAGKSYSVETVEYILNKYMKDLELY
                     FIVGQDAFQAVTTWKDWERLLLLCNFAVMTRPGYDDMRLDAILPKTFAAKFIYDKKTD
                     GFKGPTGQAIYFRHTSFLDISSSRMRDMVRTGNSIRYLTPGKVRQYIMKHNLYKECRD
                     N"
     gene            complement(45066..46322)
                     /locus_tag="PLR13_03920"
     CDS             complement(45066..46322)
                     /locus_tag="PLR13_03920"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQC18511.1"
                     /translation="MDIKDSVEHIARNAALAAKKLSRVTETEKNTALLRMAEALERNS
                     KFLLDENAKDVNGAKKAGISKAMLERLTLKQSTIDQMAKGLREVATLPDPVGKIISMW
                     RRPNGLLVGKMRIPLGVIGIIYESRPNVTVDASALCLKAGNAVILRGGSESIKSNLAI
                     ASVLRKVLQDTSIPVEAIQVIPVTDREAVAAMLKMEEYIDLIIPRGGEDLIRAVVAQS
                     RIPVIKHYKGVCHIFVDASAEMAMAVDICLNAKTQRPGVCNAMETLLVHQTIAGKFLP
                     VVANKMKKAGVVIRGCEKTKSILKRIEKAVEDDWSAEYLDLVLAVKVVENMDEAIAHI
                     EKYGSLHTESIITGDYSNAQRFLNDVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAFGPMGLEELTTTKFIIYGNGQVRT"
     assembly_gap    46407..46506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46608..47675
                     /locus_tag="PLR13_03925"
     CDS             46608..47675
                     /locus_tag="PLR13_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18512.1"
                     /translation="MSRIRIKAGRKIYQLIKDGGFNFDLISTYFGPAVGPRWLIASGF
                     DLTLLERCSLGRSTPVQLVGSSAGAFRFAAWLQPEAVKSYRQLMEAYIHVTYTKKDTP
                     ATTLEKITGIINAYLENDALPFALNNKKYRLTIITARSRGLIAFQNVLLQKTGLIFCF
                     LLNYLSRENIYKFAERVVFYNASKPPAFCLKPQFRGIYVPLNEINFKYAVLASGAIPL
                     VIEGVRDIFGAPRGVYRDGGLIDYHLTHPFASGENDIVLFFHHQERIIPGWLDKKITR
                     RAPDPNILNNVLMVFPSQYFTESLPGGKVPDRTDLVTFIDDPQTRIRNWCKAVDLSAP
                     LGDDFLELVESGRLRDVVEPF"
     gene            47707..48636
                     /locus_tag="PLR13_03930"
     CDS             47707..48636
                     /locus_tag="PLR13_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18513.1"
                     /translation="MNLEEKIFLLQEIFKDCILCPRNCHVNRTKNERGYCGLGARIIM
                     DCALAHHGEEPPLSGTNGAGTIFLSSCNLGCIYCQNHQISHHITGQEMNSRQLADVML
                     DLQKRQCHNVEPVTPTSQMPLVMEALLIARKAGLKIPFVYNCGGYESPDILKRLDGMV
                     DIYLPDFKYGQEEDSMMFSAAPGYPWFAADSIKEMVRQVGDALELKNGIARKGIIIRH
                     LVLPGRIENSKAVLRLISRKISNNVFLSLMSQYTPMAKVRNHPDLKRRITKKEYEQVL
                     DYAEKLGFHNLFIQEVSDFDLTPDFSLDNPFES"
     gene            48646..>49087
                     /locus_tag="PLR13_03935"
     CDS             48646..>49087
                     /locus_tag="PLR13_03935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18514.1"
                     /translation="MDIDIREIYDRFAQTYDKNRYLFDMTDVMDDFFRRLPSVKGSLL
                     DLGCGAGEPFPAYFIQKEWQVTGVDFSSKMLELAHRYQPAMKTIAADIREVEFPGEQF
                     DAVTTIYCLFHIEYEKHEGIFRKIYRWLKPGGRGLFTYATKAYTG"
BASE COUNT        12537 a        12550 c        11322 g        12378 t
ORIGIN      
        1 caactagcta atggtacgcg gactcatcct tcagcgaaag cttgcaagca gaggctatct
       61 ttgactaccc gaccgtggtc aggcagtttt attcggtatt agctcacctt tcgataagtt
      121 attcccaact gaagggtaga ttatccacgc gttactcacc cgtgcgccac tttactcatc
      181 ggccgaagcc gattttcgcg ttcgacttgc atgtgttagg cacgccgcca gcgtttgttc
      241 tgagccagga tcaaactctc cagttttaat cctagaagaa agatttctct ttctattttt
      301 cgcgttactc aaataaattt gtttggaccc acaaattcat tttcccacta ttcaatttcc
      361 aaagagccct atcaaaaaag agcctatgct ttaaatctaa gcagctagat tcttttgtca
      421 agctttcaaa aaaccttgtt caaccagtta cgattggaag tgtccgataa ctgcgaatta
      481 aactatcggt taactcattg aagttttcaa cttatgtcca agcacttact ctgatgaaca
      541 gaaaaggcta tttactctct ctttgtggtt gtgtcaagaa aaaatttaat ttattttcac
      601 attattgata gtttaatttt ttcttattgt tttcaatcac ttggcagact gttttttatt
      661 tcaaagggca attcttatag cgattcatac ggattgaata aattattctt tatttcaatg
      721 taatattact ttaacacatc gcagttcatc attcaacatg aacttttttt atttgcaaaa
      781 acaaagcatt atgcaatact gcttttacaa atgcagccaa ttttatcatc ataccggggg
      841 cgcagacatg aaaaaatcct atattttatc ccttgatcag ggcactacca gttcaagagc
      901 catcctttat aaccacaagg gaaatattgt cggaacagca caaaaggaat ttacgcaaat
      961 ttatcccaaa gccggatggg tggaacatga cccgatggaa atatggggat cgcaaagcgg
     1021 tgtggtcagc gaacttcttg ccaccaccgc tattgccgcg gaagaaatcg ccgccatcgg
     1081 cataaccaat caacgagaga cgaccgtcgt ctgggagaaa cgaaccggga aaccggtata
     1141 caacgccatc gtctggcagt gccgccgcac cgcggatatc tgcgatgaac tcaaggcaag
     1201 gggcctggaa aaatatatcc gggaaaatac cggactggtg cttgacgctt atttttccgg
     1261 aaccaaagta aaatggattc tcgaccatgt ggaaggcgcc cgacaaaagg cgcaagacgg
     1321 agagctgctt tttggcaccg tcgatacctg gttgatctgg aatttaacca gaggaaaggt
     1381 gcatgtgacc gattacagca acgcttcccg gacaatgctt ttcaacatca aaaaattgaa
     1441 atgggatcaa aagatattaa aggaactcga tattcccgcc tccatgctgc cggacgtcaa
     1501 accgtccagc atggtttacg gtcatacgga ttcccgtctc ttcggcgcgg aaatcgccat
     1561 cgccggagac gcgggcgatc agcaggcggc gctcttcgga caggcctgct acacgccggg
     1621 tatggttaaa aatacatacg gcacgggctg cttcatgctc atgctgacag gagagaaact
     1681 ggttcattca cagcacggcc ttctgacaac catcgcctgg ggcatcaaca acaaagtgga
     1741 atacgcgctg gaaggcagta tcttcgtcgc cggcgcggcc atccagtggc tgcgcgacgg
     1801 catgaaactt atttatgacg ccaaagacag cgaatatttt gctacgagag ttgaagactc
     1861 gctgggcgtt tatgtcgttc cggcctttgt cggattaggt gctccctact gggacatgta
     1921 cgcgcgcggc gccattctcg gactgacgcg gggaacaacg agaaatcata tcattcgcgc
     1981 gacgctggaa tccattgcct atcaaaccag ggatgttctg gaactgatgc gcaatgaatg
     2041 cgggctggat ctgtgcgagc tgcgtgtgga cggcggagcc tgcgccaata attttttaat
     2101 gcagttccag tcggatattc tgggcgtccc cgtcgagcgg ccggaaatca tcgaaaccac
     2161 ggccatgggg gctgcttatc tggcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnntgc ggaattgcga gagatcttgc gttgcgcggc attcctcact gcctcgttga
     2341 aaaaggcgac ttcgcggcgg gcgcgaccgg cgcgtgtcac ggtcttttgc acagcggagg
     2401 caggtatgcc gtcaccgatc cggtagccgc ccgagaatgt tacgaagaaa atcttattct
     2461 cagaaggatc ggcagaaagt gcgttgaaca aacgggagga ctgtttgtgc gccttccagg
     2521 agattctaaa cgttttcgcg aattatttct gcggggatgc gaagacgtcg gaattccggc
     2581 cgaagttatt tcgtcaacac tggcgatcga tctcgttcca aacctgaatc cggacatcga
     2641 ggaagccatc cgggttcccg attgttccat tgatccgttc cggctctgcc tgctcaatat
     2701 ccggacgtcc gaactgcgcg gcggcatgat tttcacacgg cacaaagtaa cggaaattat
     2761 caaatcccac ggccgctgca cgggcgtcaa ggcggttgat cagaccaccg gcgaagaaaa
     2821 agaaattcac gccaaaatca tcgtcaacgc caccggcgcc tgggcagaca tcgttgcaac
     2881 gctggccgga tccgaagtgc ctttgacgct ggtgaaggga tccctcgtca tcacgaatca
     2941 ccgcctgacc aacatggtca tcaaccggct aagacaacct tcggacggcg acattatcgt
     3001 gcccaatgaa gccgtctgcc tggccggcac aacatcgctg gccattgaaa atcccgataa
     3061 tctcctgatc gaagcttccg aagtggatac aatcatcagc gaagccggac ggaccattcc
     3121 tcatttcaac aaaacgcgca tcatcagggc cttttcaggc gtacggcctc tgctgaaatc
     3181 aaaaaaagcc gacagccatg aaatcacccg cggctttcag attatcgatc atcaaaacgg
     3241 actattgagc atcgtaggcg gaaaactttc aaccttccgg ctgatggcgg aaaaaacggt
     3301 cgacaccatc atgacaattt ttcatctgaa aaaagcttgt gaaacggcgg aaattccgct
     3361 cgacggtcag gaagagttga gtggctaccc gctttccaaa cggctcgcgg acatgaccaa
     3421 cattgtctgc gagtgcgaac ttgttaccag acaaaacgtg gagaaaatta tccaacagac
     3481 ggcaacccga aacgtcggag atatttctca ccggacgcgt ctgggcatgg gcccctgtca
     3541 gggcggcttc tgtaccttca gggcgctcgg catcatgaac gatatgagca tcatatcgcc
     3601 cgaacaatcc atggagatgc tgcgcggatt tctgcagcgg cgtttcaagg gcatccggta
     3661 cgcgctctgg ggagaccagc ttcgtgaaga acagcttgtt gaatacatct atctgggcat
     3721 cctgtccatg gagaaaccgc aatgagtgtg actgaatacg acgctgtcat cattggagcc
     3781 ggcgtagccg gactgaccgc gggaacgctt cttgccgaac gcggtttgaa agtcgcgctg
     3841 gtgaccaaag gagagccgac tgcgtgtctt tccactggct gcgtcgatgt ctgttccctg
     3901 tcaaaaaatc cactctccgg cataaggagc ttgcctccgg aacatcccta caatcttgtt
     3961 gaggaaaaaa ctgtcaaaca atctcttgaa aaatttcttg cagatatgaa agacgccgga
     4021 ttgccttaca gcggttctgc ggacgccaat cgtctgattc tttccgcgct cgggacatcc
     4081 aagatttcct gcctgatccc ctccaccatg acggcggcgc ctcaggaaaa ccatgacggg
     4141 agcgtccata ttataacgtt caagggattg aaggatttct atcccggata catcgtctcc
     4201 cggcgccgca gcacgacctt ttcggtgtat gatgcaggcg tgtccaccac catgagtatc
     4261 gccgcccgtt ttgaagagaa ggattttttc gaaagattca tcgcctggat gacgcaacgc
     4321 ggcattcacg aggacgggat cgcctttcct gccgtgctgg gcatggaatc cgcggcagac
     4381 atcgtcgaca cgatatcgga acggacggga aaaccggttt ttgaaatacc gaccatcccg
     4441 ccgtccatgc ccggcagaag acttttcaat gcgctgaaga aaacgtttcg caataagggt
     4501 ggaaccatct actggtcatg gcctgtggca ggcatggaaa agacggggaa tattgtcgaa
     4561 gcggtaacga cgccctccga aggaagaccc aacagtatca atgcccgggc atttattttg
     4621 tgtacaggct cctttgtcag cggcggtctg accgccacac gcgagggcat cctggaaaat
     4681 attttcaatc tgccggttta tgttcccggc ccccgggata cctggtttga taacgattat
     4741 ttttccctga atcacaacat cggcaaagcc ggcatcaggg ccgacgcatc catgcgcccg
     4801 cttgatgctc cgtggaaaaa tatttttgtc tgcggcagca ttctcgccga tacccaaatt
     4861 ctgaaaaacg gctgcggcca cggtctcgcg ctggccacag cccatgttgc agcacaatcc
     4921 tgcgcggagt acctgctcga tgaaatttga acattgcata cgctgtacga tttgcgtgga
     4981 gaactgtccg gtctttcggg tcaatcccga atttcccgga ccgaaacagg ccggaccgga
     5041 cgcgcagcgt ttccgctcgg aaagagacaa ggcgcaggac ggctgggtgc tgctgtgcag
     5101 ccagtgcaag cgctgcgaaa tggcctgccc ctgcggtgtg gagccggcac agatcattct
     5161 gaaagcccag cagcgatacg gcaacgaaca tccgcaaacg ccggcgcatc ttctgtttgc
     5221 caataactat tatttgagcg ctctggggtc tttcaccgct cccctggcca atgttgttgc
     5281 gtcaactcag accggaaaaa agatttttca gaaaatgggg atctcgacgt atctgccctt
     5341 tccaaaattc agcttccgga caatgcgccg ggaaaagaaa agactcaaca agggcagaaa
     5401 aaaagtcgct tttttctacg ggtgtttcat caacttttac cgccccgaca tcggcagaaa
     5461 aatcatccgc ctgctggctt cgatgaacgt cgatgttgtt ctgccgcccc agtggtgctg
     5521 cggcctgccc gcactgggca acgggaatct gacgctggca agatatttcg cgaaaaaaaa
     5581 tgccgaatcc ctgtcaaatt acatcgacgc cggttacgac gtgatttaca cctgcacctc
     5641 ctgcgggctt tgcctgctcc atgattaccc gggcattctg gaaattccgc agggcagaaa
     5701 aattgctgaa aacagttatg acgtgcatga gtacatcatc aaactcatca atgaagggta
     5761 tgtcaaaccc gacttccggg aggtcagacg cagcatcgcc tatcatattc cctgccatct
     5821 ccgggctctg aagatcggtt atccggcggc aaaactcatg tcgctgatcc ccggcctgga
     5881 atatgaaatc ctcgacgacg cctgttgcgg actttccggc agctacggtt ttaaaaaaga
     5941 aaatgctctt acggcgatac agatcggcaa ccgcgccgtc tccctgatca gcaaaaccgg
     6001 catggacaat atcgtgtcgg attgcggttc ctgccggatg cagctggccg gactttccgg
     6061 catgcccgct ttggatccgg cggaaattat ttttgattct ctgggcagat gacgtttatg
     6121 aacggtttat aaagaacaca tgctttgtcc cgctctcata ttttgatatt tttaattttc
     6181 ttttgcaccc gaaaatggca atgaatgact atgtataaga ttctgattgt cgatgatgaa
     6241 aaagatatcg tggatctcgt ttcctataat ctggagaaag agggattttc tacggtcaag
     6301 gctctcgatg gcgagtccgc cctccaaatc atcagagcac aaaagccgga tttgattatc
     6361 cttgatttga tgctgccgaa aatgaacggt ctggatgtct gtaaaaccgt ccggcgcaat
     6421 ccggaaacgt cgggtctgcc gatcatcatg ctgacggcaa aaagcgatga ggtggacaaa
     6481 gtcaccggtc tcgaagtcgg cgccgatgac tatgtcacca agccgtttag cgtcaaggaa
     6541 ttgaccgcca gggtgcgcag cgtcctgcgc cgcctgaaag aaaatgagct atacgccgga
     6601 aaagagaaat tcagtcacgc gggacttaaa atcaattacg aatcgtgtct tgttctggta
     6661 aatggtaaaa aagttacgtt aagcccgacg gaactgaagc ttctcttctt tctatcgcgc
     6721 catcccggcc gggtctattc ccgcaatcag attatcgatc atgtctgggg tgacgacacg
     6781 ttcataaccg atcgcgctgt tgatgtgcat atccggcgac tccgttccca gatcgagcag
     6841 gatattgaaa atcccaaata tattcttacc gttcgcggct ttggctataa atttgccgac
     6901 ctgaagtaat cggccgtctg ctttttttgc gtttttttct aaactttctt taagctgtca
     6961 tcaaactgta acataaattc tgtagagtaa cggcaaattc aaaaagcagg aggaaagttc
     7021 aatgttcagt attcttaaaa aagcagcctt aacgatcgca gtcgtagccc tgatgagctc
     7081 agttgctctg gccggctcat cgatcgttat caagggatca accaccgtct tgccgataac
     7141 ccagagcacg cttgaagcct ttatgaaaaa gcatcctgac gtgcagatgt cgctttcggg
     7201 cggcggttcc ggcgagggga tcaaagccct gattgataag acggcacaca tcgccacatc
     7261 atcccgagaa atcaagaagg aagagattgc gctggccaaa accaaaaatg taaatccggt
     7321 tgcccatgtg gtcgcgcatg atgcgattgt tcccgttgtc catccaagaa acaaggtcaa
     7381 aaatttatcg gttgatcaac tcagtcagat ttatcagggt aaaattacca actggaagga
     7441 agtcggcggc gatgatttga agatcgttgt catttcccgc gattcttcat cgggcacatt
     7501 tgaatcatgg gatcatttcg tcatgaaaaa aaccaaggtc acgccgagag cgcaaatgct
     7561 ggcctccaac ggcgccatcg ttacggcgat tgccaaaaat cgttatgcca tcgggtatct
     7621 cggcatcggc tatctggaca aaagcgtgac ccccttgcag gtcaacggca ttacggcaac
     7681 catccagacg gcgctttcca aacaatatcc tttttcccgc gagctctaca tgtacaccga
     7741 cggtaacgca accggcgacg tggccaagta tttagccttt gttaaatccg ccgaagggca
     7801 gaaaattgtc cagaaagaag gatttgttcc gttgacaaaa taaaatgatt ttatgacgcc
     7861 aatacgcttg aaaagggaac ctgatgcaaa atgacagtat gaagaaaaac gtattgtttt
     7921 tatgcaccca taatgcggtg cggtcgcaga tagctgaagc gtttttgaat catctttacg
     7981 gggatcgtta ctcggcattc agtgccgggt ccgatcccac acagattgat cctctcgttg
     8041 tcacggtgat gaaggaaacc ggcatggatc tcagcgccaa tcagtcgaag agtttaagcg
     8101 ttttcccgga tatgcatttt gattgcgtca tcacggtttg cgatcaggtt caggaatcgt
     8161 gtccccactt cccgcgagga gatagacata ttcataaacg tttttccgat ccgtccaaat
     8221 ttaatgggaa cgctgaagac attactcatg aatacagacg tgtccgcgat gaaataaaag
     8281 cgtgggttga aaaagaattc catcaggcaa taaagtgaaa tgttatgacc agacaaatta
     8341 aagaaattat catcaaatac atctttttta ttttttcact ggtgtcggtc ctcgttctgg
     8401 gtttgatcgt attttctctt ttccgtgaag gcatcccgct gttcggaaaa atatctataa
     8461 aagattttct tttcgggatg gagtggtatc ccacgtatga tccgccgtct ttcggcattt
     8521 ttccgctgat cgtcggctca ttgatcgtca ccattttatc aacgttcatc gctgttcctc
     8581 tgggtgtgct ggcggccatt tacatttccg aaatcgcgcc gcagaatatt aagatcacgc
     8641 tcaaatcggt tatcgaactg ctggccggtc tgccgtccgt tgttctggga tttttcggca
     8701 tggtcatcgt cgccccctgg atgcaggaaa ccttcgacct gccgaccggt ttgaatattg
     8761 tcaatgcatc gctgattctg gcgctgatgg cgattcccac catatccagt atctcagagg
     8821 acgcgcttta cgccgtcccg cgggaattca aggaagcttc ttatgcgctg ggcgccacaa
     8881 aatttgaaac cattgccagg gtcattctgc cggccgccct ctccggcatt tcgacggcgg
     8941 ttatgctggg catggcccgg gccatcggtg aaacgatggt tgtgttaatg gttgccggcg
     9001 gagcggccgc cattccggaa agtctttttg actcggtgcg gccgatgccg gcaagcattg
     9061 ccgcggaaat gggcgagacg cctttccgca gcctgcacta tcaggcgctt tttgccatcg
     9121 ggattgtgct gttctgtctg acgctggctt ttaatttgat tgccgattat gtgtcgcaca
     9181 agtttaaaca ggtcggatcc ggaacgctat aaaaggtaac gtgatgcaga tacttcaaac
     9241 ccatgcgatg aaatggcgct attttaaaca aggattgttt ttttcctgcg tccgcctgtc
     9301 ggctctgatg ataacgcttg cgctgggcgg aatcatcttg tacatcctga tcaacggcat
     9361 ttctgtaatt agctgggatt ttatcacgaa gcctccgacg gacgcgatga ccaaaggcgg
     9421 tatcatgccg gctatcctgg ggacgattta tctgaccctg ggcgctattg cgatcggctt
     9481 gccgctgggc attgcctcgg caatctatct gacggagtat gccagacagg gccgggttgt
     9541 gcggttgatc aaagtcggca tcaattgcct ggccggcgtt ccctcggttg tgttcgggct
     9601 attcggatta ggtttttttg tcattttttt aaagttcggc tcaagtattc tggccgggtc
     9661 tctaacgctg ggttttttaa ttctgccgac gatcatcggc gcgtcggaag aagcgctcaa
     9721 agccgtgccg caaacgtttc gtgaagcgtc tttatcgctc ggtgtttcca aatggcagac
     9781 aatttatcga atcgttttgc ccaatgccct gccgggaatt ctgaccggtt ccattctggg
     9841 gatcggccgg gccgcggggg aaacggcccc catcatgttt acagccgccg cttttttcac
     9901 agccaagctt ccttcttcga tatttgacga agtcatggcg ctgccgtatc atatctatgt
     9961 cctggcgacg gccggaacaa acatcgaggc aacgcgaccc attcagtacg gaacagtgct
    10021 tgttctggtg gccgttgtcc tgggtattga tctcatcgcg attatcatcc gcggatatat
    10081 gaggaaaaat aaaaggtggt aagaatggat tccagaatta ttcaattgaa caatgtgaat
    10141 ttttattatg gcgaaagccg cgccctgacc gatatttcaa tgacatttgt caaaaataaa
    10201 gtgacggcgc tgatcggccc ttccggctgc ggcaagtcca cattgctgag gcttttaaac
    10261 cggatgaatg atctgattga cgggacaaag gtggaaggag aaatcctctt tgaaagtaaa
    10321 aatatttatg cccctgatgt ggatccggtt gaaatccgcc gtaaaatagg catggttttt
    10381 cagaagccca atcccttccc gaaaaccatc tacaacaaca ttgcctatgg cccgaaactc
    10441 accggcatcg ccgtggacga tatggccgca ctggtggaac aaagcctcaa acaggccgtt
    10501 ctctgggatg aagtcaagga tattctaaat aagtccgcca tgaatctctc cggaggccag
    10561 cagcagcggc tctgtattgc ccgggcgctg gccatgaagc cggatgtgct tttgatggac
    10621 gaaccgacgt cggcgctgga ccccatttcg accgccaaaa tcgaagagtt gattgaagag
    10681 ttgaaaaaag actatactat catcatcgtg acccataata tgcagcaggc agccaggatt
    10741 tccgatgaaa cggcgttttt ttatctgggt aatctggttg aatataataa aactgaaaaa
    10801 atatttacca atccggatgt caaacagacg gaagattata ttacaggaag atttggctaa
    10861 gcattaaccg gaggatcatc tatggaagaa agaagacata ccagttcaca ttatgagatg
    10921 gagctgcagg acataaaaaa caatctgatt tatctcggcg cactgacgga aaaagccatc
    10981 cattcagcca tgacatctct gacggacaga gacacggatt tggcgaagaa agttatcgcc
    11041 aatgattatg aaattgataa aatggacaag gatattgaag agaaatgtat taagatgctg
    11101 gcgctgcgcc agccgacagc catcgattta cgattcatca ccacagccat aaaaatcacc
    11161 ggccatctgg aacgaatcgg cgacatggcg gtcaacatcg cagaaaaagc catacaactc
    11221 aacgaagaac ccaaactcaa accgtatatc gatttgccca atatgtcgtc cattgtggag
    11281 aaaatggtgg ctaaagcgct ggatgccctg attaaacggg atttacagat cgccgatgat
    11341 gtccggcaga tcgaacagga agtggacgat ctcaacgagc agatattccg ggaacttttg
    11401 acgtttatga tggaggattc caaatccatt catcgggctt tattaattat gcacgtttca
    11461 aaaaacctgg agcggattgc cgatcatgcc caggggatcg cggatatggt aacgtacatg
    11521 atcacgggtg aaaacgtccg tcatctttcc agggatgaaa aatagaggca tatcgagtga
    11581 aaaaaaacct gttctataaa atatttttca gttatctggt catcatctgc ctgtctttct
    11641 ttcttctgga tatttttgtg cgggacgaag tcagaaaagt tctgacgtca caaattgaaa
    11701 cggaattatt gtcctatgca caactgattg accagacgtc tctggaaaag gcatccggcc
    11761 atctgaagca ggttgcccaa atctcccagt cgcgaatcac attcatggac gcccggggca
    11821 gggtttttgc ggattcggaa aaagacattg ctctgctgga gagtcatttt aaccgcccgg
    11881 aagtgcagga agcccggctc agaggcacag ggaaatctgt ccggtacagc gattctctcg
    11941 ggatcgatat gctctatatg gctgtcgccg tcaaagacgc acagaagatc aagggatacg
    12001 taagattggc gcgcccgctg catcacgtac aaaacgttat cgacaaggta tatcagtaca
    12061 ttctcctgac tttattcgtc gtggcgctga ttgcgttggg aattgcactg ttcttttcat
    12121 atcgtttgta cgaaccgata aaaatcatgg aacaatttac cgaaaaatta cggcgcggag
    12181 atccggtcgg gtccatcatt ctggatacgt cggatgaaac caaaaccctg gccgataaca
    12241 tcaattatct ggttgaagaa ctgcgggaaa agataaacat cgccaacgaa gagaagaaca
    12301 agctgatgac ggctttcacc agcatgaacg aaggcgttct gattcttgac gaacaaggcg
    12361 tcatcgaatt cagcagcccg gttttaagta acatgctggc gattcaatac gacgatgtcg
    12421 tcggcaaaac actgatggaa gctttccgca atattgattt gcagaaggca tttcaggagt
    12481 tcaaggaaaa acgcatcagc gtgtcgcgtg aaattatcct cggccatgcg gataaagtcg
    12541 tgctgaatgt cagcctctcc gccgttcacg gccgcccgga tgaagagaaa acgatgattg
    12601 tctttcacga tgtgacacgc ctgaaaaaac tggaacaggt ccgcatggat tttgtcgcca
    12661 acgtgacgca tgaaatcaaa acaccgctga cggcaattat cggctatctg gaaaccattc
    12721 aatccggcgc gattaccaat caggaggaaa cgaaaaagtt tatcgacatc attttgaaac
    12781 aggccgaacg cctcaaccgg cttgtggaag atctgctgac gatttccaat attgaaacga
    12841 aacaaaccgt tttgaatttt gaaagcgttt ctctaagaaa ggccatcgac actatgctgt
    12901 cgttgattga agcgaaggcc gaactgaaaa atatatccgt gcgtaataat gtcagcgata
    12961 atttccctcc gattacggcg aaccgggaca ggctcacgca gatctttgtc aatattctgg
    13021 acaacgccgt caagtttacc ccgaacggcg gcgagattag tattaacgct tctgaatcgg
    13081 gatcttacac ggttgtatcg atccaggatt ccggtatcgg cgttccgaaa gacgacatca
    13141 gccgtctggg ggaacgtttt tatcgcgttg acaaatcacg ttcacgcgat ttgggcggca
    13201 cgggtctggg attatccatc gttaaacatc tcatgattgc ccacggcggt agaatggaaa
    13261 tcgaaagcca accgggcaag ggtacaaaag tatctcttta tttccctgta aaatgaaagc
    13321 aatggacgat cgttttttta cttaaaatag acggcgtttt ggaattgtcc gtctgcagga
    13381 ggatgccgta ttaagaaaaa atgccgtttt gccccatatc ttgtggtcaa aatatccttg
    13441 acacacaaga taacgtttct tatactggaa atgccgaaaa tgaagttctt atcaactcaa
    13501 taacgaaacc ggctggtccg gttcaggagg tgtgcccgca gaacttctac ctttcataaa
    13561 tcacagttca ttaatcccag gagcgattct atgacgaatg ccaaaagagc tgaggtgcac
    13621 gcaatcgcaa tggagaccgt caaggcggca gagaaggttc cggaaaagta ttttgtggtg
    13681 gataccagcg cgctggaata cggaatggat gtttttgaaa aattgcgcca gggaggccgc
    13741 aattgtgtgc tgattcctta tgcgacgtgg gaggaactgg acagccataa aacaggaaac
    13801 gatttcaagg cggacattgc ccgcaacgtt attcgcaatg tccgtaatct atttcgcgca
    13861 aaagaccatt ccgtccgttt ttttcagtat gatttcaatc tggtgaaaga cgccgatctg
    13921 aaggtcgata aaaatgatca ctgtattatt gccaccgcac tcaatgccaa aaagaaactg
    13981 ggcaaagcca aagtcattct cgtgactcag gatgccgcgt tggaggccct ggctctgaat
    14041 ttcggtctcg atgtgcaatc catcaaaatg catcagacag acatcaagca gcaccatacc
    14101 actcgcgcga cgattaatct tcaacggagc tggatttcgg ataaaacctt tcccttgaaa
    14161 ctgcccgaag ccaaactggc tgcgcaactg gaacaggtcc tgtataatga aggcatcatc
    14221 tgttactgcg accttgatgg cgtctgggga ccggccttcg ttgccgtacg caagggcgac
    14281 tatttccgta ttatcgaccg ggacattcat ttgctgggga tcaagccgcg ttccaataac
    14341 ggcgatgtca actggggcca gtttatcgcg atggatttac tgcgcgatgc aactgttccc
    14401 ctggtgacgt tgaccggtaa ggcgggaacc gctaaaacct ttctcgcgct gctggccgct
    14461 tttgaacagg cgtcgcacta ccggcagatt ctggtcagcc gtgccaccat ccaactggga
    14521 aacaaggacc ggctgggtta ttcgccggga gacatcgacg caaaaatgaa accctggatg
    14581 catccgatct acgacaatat caatgccatc aaagaactgg ttgacgaaag gatgaaagaa
    14641 aagatcaacg actggctggc caacggaaaa atcgaaatga tggatctgga caagattcgc
    14701 ggccgctcca ttcaaaaatg ttatgtcatc gtcgacgaga ttcagaacct tccgccatcc
    14761 catgtcaaag ccatagcgac gcgcggcgcc gagggaacaa aaatggtttt caccggcgat
    14821 ttctcctacg atcagattga tgttccctgg ctcgacgaac gctccaacgg cctttcggtg
    14881 ctcaacgcga agatggcggg cagccgtttc ttcggcagcg tgcatctgga tcaggccatc
    14941 cgttcggaac tcaccaagga aattttggaa cgctggtaaa aaaacgctgc gtgaagggac
    15001 aagtgctttt cttgtgattc gatgctcttt gtttatgtcc catgcgtcga ttttaatcga
    15061 aagattcatc atccggtgcg atgctttgcg gtttctcttc acgacaaaaa tttacgttga
    15121 gcaatcctca catggttgac catcgtctgc ggccggaaga ataaaaatcc gaggcgtcca
    15181 tgcacaatag aaaaatattc ttgcatttcg tactcaaccc ggacccgcaa agagaatcat
    15241 gtccggatag atctgtcgga aggtctccgt ttctaatttt cctgttttat gctaaattaa
    15301 aactagcgta ttgaaatacg gggtaattcc ggtctgacaa gaggttcgtg tttgggagga
    15361 tgatccgtca gactgcaagg actttgttaa atttgaggag gtgttttatg gcagacagca
    15421 aaagtgattt tttcacaggc ctgattgtcg gcggcctgat cggcgtagcg ttgggaattt
    15481 tgtttgcacc caaaagcggc aaggaatcgc gcgaagatat tgcccgcaag gcggatgaac
    15541 ttatggccaa agcacaagaa ggatatgaaa aagctgcggg aaagtactcc gagatgacag
    15601 gcgtggaatc ttttcgcgat tacaaaacag cagacaatga agatttggcg taatgaacgt
    15661 ttacaggcaa tcttccgcaa ggattgtcat caatggctgt tcgggcgtct caatgtgcat
    15721 ttagagagca ttgagacgcc tggccgtcat gatctccgga cgattgccca aggcgcggtt
    15781 tttaaaacga gtcgaacctg attttcacga tgccccttgt cagaggggta tcgcgcttcg
    15841 ccacgaaacg tctcacacag acaaatgccc ctcaataatg aaacgaattt ccgatttatg
    15901 gatggcgaaa cggcattaat tttttactac ctcaaaggaa acctgaacag ctacaacgcg
    15961 ctggccgggg ctctggaaaa gtatccggac atcaaaaaca tcgacgtgta ttttctatcc
    16021 tctgaaaaag aacttcttca aaaaatatcc gccatttcaa aaaagcacag agaaacaatc
    16081 attggaatct ccttctgcac gcctcagttc ctgaagatca aagacctgat taacagggtg
    16141 aaaaaattag ccggcgaaac cgtctttctg gcaggagggc ctcatcccgc aggcgctcca
    16201 ctggagacgc tgaaactagg cttcgatttc gtaatcagcg gcgaaggaga agaaacgctt
    16261 acggaattac tgtccaaaat taatcatgat gaaaataatt cggacataaa aggtttgtca
    16321 tacctggaca aaaacggaga gtacgttttt accggcaggc gcgaagcagc agacatcaac
    16381 actttttatc ccatctcgca ctatttttcc aaatacggcc ctatcgagat tacgcggggc
    16441 tgtccttatg cctgccgtta ctgtcaggtc tcgcagttgt ttggcacaac accgcggcac
    16501 cgcagtatag aaaatattct ggattgcgtc aaaataatga aaagacagga ttcgacgtat
    16561 tttcgattta tttcccccaa cgctttttca tacggctcgg aagacggcaa aagaatcaat
    16621 aatgaaaaac tggagaacct gttgtcggac atcaggcgcc tggtcggaaa gagcggtaaa
    16681 atattcttcg gcagttttcc gtcagaagtc aggccggaac acgtcaatgc ccatacggtc
    16741 aagatggtga aaaagtacgc caacaacgac aatctgatca tcggggcgca atccggcagc
    16801 cagcgaatgc tggattgcgc cggacgcgaa tatgatgtgg caaccatata caacgccgtg
    16861 gatgtcaccc gtcaggccgg tttgaaggcg aaagttgatt tcatattcgg cctgcccggg
    16921 gagacagacg aggatattgc ctccacgctg aacgttatac aagacctgag ccggtcgggc
    16981 gcaaaaattc acgctcatac attcatgccg ctgccccgga cggccttcga aaacgaaacc
    17041 gccgggaaaa tcggtccggc gctgaaaaag gaactcaacc ggctttgtgc taacggcatc
    17101 atctacggcc agtggcagca acaggaaaaa atagcggcgg gaatcgcggc aaaacaggac
    17161 aatgcctgat catccgtcgt catttcaagg ccttttggtt ttcgttgatc agcgcttttc
    17221 tggatattcc cagatcaata atatcccagg gcagaatttc atcgaaagat ttctcacggt
    17281 aaacgtaaaa atccggattg acgttgactt ctttgagtcc ccggccccag ttgccgtcca
    17341 gacgattgac ggccagcagt atttctccaa cacgccggtc gcccagcgca agcagtgcct
    17401 gaacatagtt ccatttcggg acgtcgtgaa tcacgcggac agacttgtct tttcgaaacg
    17461 cctctttgat ctttttgatt cttttgccgg cgatatggac gtcgagcaga ggaacccatt
    17521 gaaacggcgt tgccggtttg ggaataaact gattgatgct cagcgtcaag cgccggaact
    17581 gttttttccc gtcgaaccgg agacgtacat ggtgctgaat ttttttggtc agaccgatga
    17641 tggcatcgat gtccgactcc tcttcccgtg gaaggccgat catgaagtac agccttatat
    17701 tggcaatgcc atgctctgcg agtaaggctg tggcatacat gatttgctct tcactgattt
    17761 tctttttcag gatatcgcgc atcttctgac tgccggcttc cggcgccagt gccacggtct
    17821 cgatgccgtt tgcttttatg agaccgacca tctcctcatt gatccggtcg attctcaggg
    17881 aaccgagccc cgcccggccg ttatgggcaa tgatgtaccg gcagacggcg gggatgtccg
    17941 gatgatcgga aacggcggtg cccacgagcc cgattttatt ttttcgcgtc aggccgtcat
    18001 gaattgcgcc gatcatttcc tcaatgccgc ggaaacgaac cgggcggtaa atgaaactcg
    18061 ccgcacagaa tctgcagcca tgtgcgcaac cgcggttgac ctcgaccagg aacatgtcct
    18121 ccatctcggt gcaagacgcg ctgatcacct cctgtgtgcg gaacgcattg atatcgttta
    18181 caggctccac ttttattttt tccggcaacc ccgcttcccg cggaaccacc gatgtgattt
    18241 ttctttccgg cgaatagtga acgtcgtaga gccccggaac gtaaacatga tgcagttgct
    18301 tttgcgcatc tttcagaaat tgttcccggc gctgccccag acggcgcgat gcttcaaaaa
    18361 aacggcagaa taccggcaaa atgtcctctg cttcccccag aacaaagaga tcaaagaaat
    18421 cggccagcgg ctccgggttc aacgtcggag caataccgcc ccccagaatc agaggatatt
    18481 tttccgagcg atctgcggat cgtagcggca taccggcatc atccatgatt ttcagaatat
    18541 tcgggtaatc gttttcaaag gagatggaaa acgccaggat gtcaaattcg gttaatgctt
    18601 tttgattttc caggctgaag attcccgccg ttccggaaac gcatctggcc tcgtttccgg
    18661 aaacggacag gaatgccctc tcacaaagaa aggaaggttg agcatttaat agttgataaa
    18721 cggtttgaaa ccccagattc gccatgccta tcctgtagtg attgggataa acaaggcaaa
    18781 cggtgttgtc tttcccccat atttttttta catatccggt ttcattctcc agtatgcttt
    18841 tgtatactgc ttttagtttc caatccatgg cttgatcacg gcctgcccgt gaaacggaac
    18901 atcccggata acgtttctgg tgaaagcatc atgatcagtc agcctgacgc cttaaaaacg
    18961 tgggaatctg aatatccata ttctccgttt cgtcgccaag acccacaacg gctatttcac
    19021 cgctgttgag ttttctttcc cgacggataa acgtcggggt agcaagatcc tcccgtccgc
    19081 cgacgccgcg gaaccgggta acgttgccga ctgaatcatt tttcttcctg gcgaaatcat
    19141 caaaaccggt ggcgataacg gtaatgcgga tttcatcttc catgttctga tcaatcaccg
    19201 tgccgaagat aatattggcg tcttcatccg cctcttcctg aatcagggac gaggcctcgt
    19261 tgacttcata gaggctcatg tccgacccgc cggtaatatt gagcagaata ccgtgggcgc
    19321 cctgaatggt attgtcttcc agcaacggag acgaaatcgc tctctgggcc gcttcgacgg
    19381 ctctgttttc accgctggcg atgcccgtac ccataatcgc catgcccata ttgctcatga
    19441 cgcttctgac atcggcaaaa tccagattaa tcaggcccgg cactaaaatc aggtcggaga
    19501 tgcccttgac ggcctgataa agaatgtcat ccgccttctt aaacgcttca agaagcgaaa
    19561 ggttgcggcc tcccaggctt aacaatctct gattcggaac aacgatcagc gtatccactt
    19621 tttccctgag ctgttcgatg ccttcctccg cctgcatatt gcgctttttc ccttcaaact
    19681 ggaacggctt ggtcacaacg gcgatggtca gaatgctgca ctcgcgggca atctctgcaa
    19741 tcaccggagc ggcgcctgtt cccgttcctc cgcccatgcc cgccgtgatg aaaatcatat
    19801 ccgcgccttc cagataagga cggatttcat cgcgggattc cagagcggat tgtttcccga
    19861 tctccggatc ggaaccggct ccgagccctt tggtgatttg acttccgatt tgaattttga
    19921 ccggtgcgct ggacacgccg agggcctgcg catccgtgtt ggcgttgata aaatcaacac
    19981 cctgcaggca ggatgcgatc atcgtgttga cggcattgcc gccgcccccg ccgacaccaa
    20041 tcactttaat ttttgccaga ttttcactac agacttccgt taattcaaac atgcgctctc
    20101 cttcctttca gaaaaattct gaaaacattt ttttaacagt tcttgttata cttccaagca
    20161 tcccggtgga tgttcctctt aaaatatttt gattacggcc gtaaatgatc agtcccacgc
    20221 cgatggcatg agccggcgaa ttgacaatgt cgttcaatcc tccgacgccg atgggaatgc
    20281 ccttgcgcgc cggcatatca aagatctttt cagccagttc gctgatgccc tccagcaacg
    20341 atgtcccccc ggtcagaacg acgcccgcgg ccagcagatc ctcaaaaccg aatttcacca
    20401 cttcgttgta ggccaggttt aaaatttctt ccatccgggg ttcgacaata cgccccagga
    20461 tctgccggga gacttcgcgg tcttcccttc cgccgaggct gggcactcgg atggtctcat
    20521 ccttgggaat catggatgtc atggcacagc cgtgtttgat tttgattttt tcggcttcgc
    20581 tcagaggcgt acgcagaccg gtggatatat ccgaggtcag ataattgccg ccgaccggca
    20641 gaatggccgt gtgtttgatg ctggattcgg aaaaaatggt cacggatgta ttggaccctc
    20701 cgatatccac gaggacgacc cccagttcct tttcatcgct gctgagcacg gcttccccgg
    20761 cggcaagttg ctccaggacg atgccgtcaa tatccagacc gacgcggttc accgattttt
    20821 caatatcctg aatggcggca atcgcaccgg ttacaatgtg aaccttggct tcgagacgga
    20881 caccggacat gccgatggga tccttgatcc cttcctgacc gtcaatcacg taactctgcg
    20941 gaagaatatg caggattttc cgtccgacag gaatggcaat ggcctcggaa gcttcaatca
    21001 ctctctgcac gtctttttca tccacttccc ttcccttaat ggaaacgata cttaaagagt
    21061 tctggccttt aatgtggctt ccggaaatcc cgacaaaagc cgaattgatg cggcacccgg
    21121 acatatgctc cgcttcctcc accgccgtct tgatggactc caccatggtg tcgatattga
    21181 caaccgtgcc ttttctcatt tctttggtct gcgatgttcc gatcccgatg atatcgatac
    21241 ccgtttcggt tacgtcaccg acgatcgccg tggtttttgt cgttccgata tcaagaccaa
    21301 ctaaaacatt gcttctcttt cccatcagcc ttttcacctc ttttttattt atactttcta
    21361 gatcagataa tttttattct gatcgccatc ttgcgttttt tccggaacat atttgcgttt
    21421 gaccgtcact ttggaatgat ccgacaaatc aacacacaag aaacctgtct tcattcccct
    21481 gttttccaaa tcggtcagaa tggcctttaa cttcctcagc ttattttcaa aatcatccgt
    21541 gcccagtttc aaatacagac cgttgtcgga caccagcgca agcccgaaca cggaatcgat
    21601 atgaatctcg gaaatcgttc cgagatgagc atacgcggga gatttagaaa tcctctggat
    21661 caaaccgagt gcgctcaaca gcagcggcga acgggttgct tcgcctgttg cagcaccggt
    21721 aatgaccggc agatcaactt catcgcttcc acccagtctt ttaaaaacat gacctttcac
    21781 atccatcaga taaaaatcac caccccgctt taccagggcc agaggaattc tctcccggac
    21841 gtccaggacg agttttcccg gcagttcctt gcccacgtaa accgtttcca cccagtgatt
    21901 ggaacagaca cggcggatga tcgaattttt gttcacggcc agaagattct gttgtggttt
    21961 aatattcgcc gatgccagaa tatccttctc cgtcagttcc ttcagtccgc gcacggatat
    22021 ttccttgatc tgaaaatacg ggctgctcat catcacattg taaacataaa taaacaggac
    22081 gctgaggaaa acagccatgc tcagcaatgt catcgccgtg aaaaaatctc cggacaagct
    22141 cgtaaaacgc cggcggattc tgttttgttt tgtttgaatg cccgttttct ttaacatctg
    22201 tcttccccga tcacgacaac ctcggtttcc agatcgatat ttttttcttc tctggctctg
    22261 gactggacca gacggatcag cgccagaaca tccgcagccg ttgccccacc tttattgacg
    22321 ataaagttgg cgtgtttgga cgatatttcc gcgccgcctg ccttttcacc tttgagtccc
    22381 atgtcctcaa taatctttcc agccggatgt ccgggaggat ttttaaaaac cgatccggca
    22441 ctcggatatt ccaggggatg ttttgcctgg cgccattgta aaatttcgct gattctcgct
    22501 ttcattgtcg cgcgatcacc ttttttcagt ctgaaccggg cccccaggat gattgttccc
    22561 gagggaaaag acgatgtgcg ataaccgaaa gagatcgctt cccggatcat tgtttttttg
    22621 ctgccttcgg tatcgagcac cgttaccgat tcaatcacat ccttcatttc ccgtccgtac
    22681 gctcccgcgt tcatccagac ggctccgccg acactgcccg gaatgccgga acagaactca
    22741 acgccgctca acgcctccac gaccgaccgg ctgacaacct tggccagaga ggctcccgtc
    22801 tgggccagga tataaccttc atggcccccc acaggtttgc actgaatttc cttcataccc
    22861 gtcatcaata tgatcgctcc ccggtatccg ccgtccttga caataacatt ggtcagattt
    22921 cccgccgtga aataatttat gccctccatc ctgaatttcc tgatgacccg cgccagttga
    22981 ttttcgtgct ccacgacaac cagggcatcc gcctttccac ccacggccag cgatgtatgc
    23041 agcgacatcg gctcgtcgta caatactttc ccggcatcta tctcctgtaa aatgcccctg
    23101 atctcacaat ggctggtcat tacgtattct ttcccctggc taccagttga gtaatgaacg
    23161 tttctcctat tttataaata ctgcccgctc cgatggtggc aaccgtatcc ttctgatgaa
    23221 ccgtttttaa gagatattcg acggtcgctt ccgcacccgg aatatattcc acatgcttat
    23281 gaccggcctt ccggatcgcc tcgcaaagcg cggcagcggt aatacctgga atcggctctt
    23341 cactggccgg ataaatgtca ttgagaatca gaatgtcaac ctcgtcaaat gctttcgtaa
    23401 actcctcaaa aagcgcccgt gtccgtgtat atctgtgcgg ctgaaaaacg acgatcagtc
    23461 tgtccttcca gatttgccgc atggccgcca gcgtcgcctt gatttccgtc gggtgatgac
    23521 cgtaatcatc aagaacagtt acttcgccga cccttccctt gatctccaac cggcgctgca
    23581 ccccggaaaa acttttcaat ccttctttga tggtggccag atccaggttc aactcccggg
    23641 caaccgctat cgccgccaga gaattataaa cattgaacag accggggaca attaattcga
    23701 tcttgcccaa tttctctccc ttatgaacca gactgtaaca cgtttttctt tttgtaaatt
    23761 ttatatcctc ggcgctgtaa tccgccgggg tatcgatacc gtaggtgatc atccgtcgtt
    23821 tgatcgccgg aagaatctcc cgcacatgaa tattgtcgtt acacaggacc gtgcacccat
    23881 aaaagggaac gatattggca aacgtcagaa acgcctcctt gatttcggcg atgcccggat
    23941 aataatccag atgctcccgg tcgatattgg tgatgacggc gatggtcggc gacaatttca
    24001 gaaacgaacc gtcgctttca tcggcttccg ccacaatgat ttcgccgtca cccaaccggg
    24061 cattgctgcc gatgctggcc agtttacccc cgatgaccat ggtcggatcc agattcccct
    24121 ccgccaggat ggtggaaatc atggacgtgg tcgtcgtttt gccgtgactc ccggaaacag
    24181 ccaccgaaaa tttcattttg agcagttccg ccagcatttc cgcacgcggg atgaccggaa
    24241 tatttttccg gtgcgcttcc agcacttcgg ggttatgctc cttcaccgcc gttgaggtca
    24301 ccacaacatc ggcattcccg acgttctccg ccgcatgtcc gatgacgatt ttagcgccga
    24361 gcttctgcag ccgggcggtg gtttccgacg gttgaacatc tgaaccgccc acattgtatc
    24421 cgagattaac cagaacctcg gcaatgccgc tcatgccgat tccgccgatt ccgacaaaat
    24481 ggatgcattt gattttgcgc tgcatcaggc ttttttcctt atcttttttc attgtttttc
    24541 cgctttccaa gcactctgtc tttaatgttt ggcgtccgtc agtttcatgc attcatcaac
    24601 gatcgttgcc gccgcctccg cttttcccat cctcagggaa ttttcttcca tcaatagccg
    24661 ccggttttca tcacgcagaa tgccgtcaat ggcgttgaac aggctttcgc cactcagctc
    24721 tttttccgga atcaccaccg cggctcccgc gtcggacatg gccagagcat ttttcagttg
    24781 gtgatcattg gccgcccacg gatacggaat caaaacagcg gccttgcccg caaccgttat
    24841 ttccgccagc gacgtcgcac cggcgcggca aatgatcaaa tccgcctgcg cataggcttc
    24901 agccatatcc atgatgaaat ccgttgctac cgcctgaaga ccggattgcg cgtaagcttt
    24961 tttaacttct tccatctgcc gcgcaccggt ctgatgcaca atgcgtatcc ggtctttcat
    25021 gccgcttaac cgcggcaaca tatcgatgac gcccctgttg atcgcttcgg caccctgtga
    25081 tccgccgaaa atcaggagtt gccggtgcgt tctgtcttct ttcgttcgga aactctgtgc
    25141 ggcaaacgac gccctcaccg gattgccgct gatcacaact tttcgacgcg ggaagcattg
    25201 ctgtgtccgg gggtaagtga caaatatttt gcctgcaacg tttcccagaa ttttgttggt
    25261 gacaccgggg atggcgtttt gttcagcaat ggacgtcggg atccccatca gatacgccgc
    25321 cagcaccgca ggacccgatg cgtaaccgcc gacaccgacc accatgtcag gactgaattg
    25381 tttcagaatt cctctggcct gccacaaact ttttggaatc tggaaaacac ttttcaccaa
    25441 tgccctgatc ccccgtccct tgatgccctc aatgtcaatc gtctcgaggt catagcccaa
    25501 tcggctgagc aatcggcttt caatgcctct tttcgttccg atgaacacca ccttggtctg
    25561 tgcatttctt tttataaatt cctccgcgat ggcaatcccg ggaaacaaat gaccgcctgt
    25621 tcctcctccc gcaattacaa tccgcattgt tcgctccaca caaagccact catgttttta
    25681 aataatatca tcacaccgct ctcacgatgt ctgcgtggaa atattgagca atattcccac
    25741 ggccgtcata ctcatgataa atgccgttcc gccatagctc aaaaacggca gaaccagtcc
    25801 cttcagggga ataagcccca tcacaccggc aatattgatg aatgcttcca tgcaaatcac
    25861 catggtcagc ccggacgcca gcaaacttcc gaacagatcg ggggcgttca gcgcgatccg
    25921 gaaaccacgg taggcaaaaa gggcaaacat ggtgatgacc atcgccacac cgatgaaacc
    25981 gctctcttcg gcaataatgg aaagaataaa gtccgtgtgc ggttcaggca ggtaaaatag
    26041 tttctgcatg ccgtcgccga tacccacgcc gaatgtcccg ccggaaccga aagagatcag
    26101 cgattgaatg atctggaaac ccgtattgtc cgcatctttc cagggatcca gaaaagcggt
    26161 cagtcgcgtc agcctgtatc ctttatacag gatcagagcg atgcccacgg gaatgaaggc
    26221 cccgatcagc agcgcaagat gtttgatctg cgcccctgca atacacagca tcatcagcat
    26281 gatcgtggca ataatcgccg ttgttccaaa atcaggttcc agcagaatga gcatcatgac
    26341 caccgacgtg gcagccagcg ggacaagcac cccctttgta aaatttgtta tctgaaaagc
    26401 cttccgtgtc agcagatgcg acaaaaaaat cacgatcatg atttttacca tttccgtcac
    26461 ctgaaaagaa aagcctccca gattcagcca gcgggtggcg cccccccggc gcatgccgat
    26521 acccggcaca aacaacaggg acagtaacag caggcacagc agcattcccg gataggcgat
    26581 ttttcttaaa tgcgtatacg gaacttttgt catcgcgacc atgatcgtga atcccagaaa
    26641 cacaaacacg agctgtttct ttaaaaaata ctggccgtcg ttgaacttct ccagggcaat
    26701 gatggaactg gaggaataaa tcatggcggt tcccaccacc gccagcagca gcgtaaccag
    26761 cagcagaaca aaatcgggat aattgtcttc ttttttgtac gcggtttcca tttacagatt
    26821 ttccaccact tttctaaaga aattacctcg ttctttataa ttggcaaatt catcaaaact
    26881 ggcacagccc ggtgataata gcacgacgtc gccgggcacg gcttcctgat acgcaacttc
    26941 cacggcttcc tgcaattttg tctttttgat gaccggaaca gtttggccga tcaatgaggc
    27001 aatgcgttcg cgcgcttcgc cgaataaaac gatttttttg gctttggccg ccagcaccgg
    27061 ctgcagggtt tcgaaatccc cgtctttgtc ccgtccgccc agcagcaaaa tcaccggctg
    27121 ggtgaacgtt tccagcgcgc gcaggaccga tccgacattc gtgcccttgg aatcatcgaa
    27181 gaacttgacg gaattctttt cttccacaaa ttcgatgcgg tgaggcaacc cccggaagtt
    27241 ttcaacagcc cggatcgtat ccgacggatc acatccgcag aaacgagccg ctaaaatcgc
    27301 cgccatcaca ttctccacat tgtgcaagcc gggaatgttg atcatgctca aaggatattt
    27361 ttcttcttgc atccccggca gactcaaaac aatattctct tctttaacaa atattcccga
    27421 gggcagaaca tgctttgaac tgaatccggc aacacgcgcc ctgatgttcc gttcgatctt
    27481 cttttgttca tcgtcttcgg cattcagaat cgcgaaatct tttccggtct gattggcaaa
    27541 gattctggct ttgatgcggc gatactcggc aaaagacccg tgataattga catgatcgct
    27601 ggtgatattc aaaagaacgg caatgaaggg ccggaattga tcgatccact gcagttgaaa
    27661 actgctgatt tcagccacga taaaatcggc atcctgattt ttatctgcgt attcaatcaa
    27721 cggattgccg atatttccgc cgacaaaaac tttcttcccc gatttctgca aaatttcacc
    27781 cagcaaggtg gtagtagtcg ttttaccgtt ggtgcccgtt acggcgataa tcggaaccct
    27841 gataaaccgg taggccagtt caatttcact gacgacggca atgttcttct tcaaggcctc
    27901 cgccagcaaa ggattaccgg gaggaatgcc gggagaagga ataaccatat ccgcgtcgtt
    27961 taaaacatcc acgctgttat attcgccgac ttccagccat ggctcgccgg caatttgctt
    28021 aaatccgcca tcccaatggt ccggtgattt ttcatcagtc accacgatnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag atccgacatc gcccatgaat acttccgcgg
    28201 gataagcgtt aaaccacaaa aatccgagtc ccgcaccgac aatcgccccg gcaaataccg
    28261 ccagttctcc tgctccggaa acatgaacaa tctgcagata attcgatgtc tttatatttc
    28321 cggcaaaata cgcaaaaaga atgtaggtgg caaaacaaat cacgaccgga ccgatggcca
    28381 gaccgtccag gccatccgtc aaattgacgg cattggccgc accgacaata ataaaggtcg
    28441 aaagcagaac atagccccaa ccaagatcag gcaataccgt tttgaaaaaa ggaatcgtta
    28501 cctgcgcgtt aaaacctggc ttcacataaa gaatcacact gacaaacaga gcaatgataa
    28561 tctcggccgc cagtctcgtt tttccggaga ctccccggga actcttgccg gccagtttgc
    28621 tgtaatcgtc gataaaaccg atcaaaccga agccaagcgt caccatgagt atcagccaga
    28681 catacacgtc cgccagattc gtccagagca acgtggatac gacgaccgcc ataataatta
    28741 acatgcctcc catggtggga gtgcctttct tcaccagatg actttgcggc ccgtcatccc
    28801 tgatctgctg accgatctgc atgtcctgaa gcttccgaat catccatttc ccaaaaatca
    28861 gacaaatcag cagcgccgta atggcggcaa agatcgtcct gaacgtgata taacgaaaaa
    28921 cgttaaagaa cgaaatggtg gtatgcagcg gatacagtaa atggtaaatc aatcttcttt
    28981 actcctgatt aatagtctgt cttgttctcc tcgctgactt tatctctgcc gaaatcttca
    29041 cagatctgcg ccgcaattct gtccatgttc atgcggcgcg aacctttcac cagaatccag
    29101 tccccttttt tcaggcattt tttcagaaaa tcagtaccgt tttccttatc ggacaaaaag
    29161 aggatgctgt cggacgccat gcctccttcc gtcgcgccgg cagcggtcat tccggcatag
    29221 tcaccctgga gaaacagcgt cttcacaccg atggtggcaa tcagcattcc tatcttccgg
    29281 tgcatttcat cggcttgtgc gcccagttcc agcatgtctc ccagaaaaac gtacgcatgg
    29341 tgattgccct ttaaatcttt cagggtcatc agggcttccc ggaccgatgc gggattggca
    29401 ttgtaggaat catccagaag atacgcgccg ttgcggagtt tgataatttc catccgtccg
    29461 tgaaacggac ggaacgccga gagaccttcc gcaatatttt tcagatccat ccccgctgcc
    29521 actgccaccg ccgcagcggc catcgcgtta ttgatatgat gaagtccggt tattttcatt
    29581 tccactttct gcgtattgcc ccggatagca aggttgaaac gaactccttt cgcaccgttt
    29641 ctttcgatat caaagacggt cacatccgca ttggggctca tgctgaacgt gatcttcctg
    29701 cctttccacg tctcagcagc cgtcaggatg gcttcatcat ccagattgac cacaatgatt
    29761 cccgacgatg gcatattcag aaataaatcc cctttctctt ccccgacaac ctgcagggac
    29821 ccaaaccctt ccagatgagc cggtcccacg tttgtaatca atccgatatc cggttcggca
    29881 atccgggtca gtcgtctaat ttctccccgg gtattcgtcc ccatctcgag aaccatcatg
    29941 tcatgctgat ccgtcaggcg gaatatcgtc tgcggcaatc cgatcaggtt gttcagattc
    30001 ccttccgtct tcaggatatt ttttttcaac ccgaggatgg aggctatcat ttccttggcc
    30061 gtcgtctttc ccgaagatcc ggtcaatccg atcaccggaa tggaaaattt ctttctccag
    30121 tcatgcgcca tctctcccag cgcgaataat gtatcggcaa cctttattac cgcaaaggta
    30181 tcatccactt tcatccggcg tattttttca tcatcctgcg ccagtattcc ggcagcgcct
    30241 ccctgaaaaa ccgactcggc ataatcgtga ccgtcaaaat gatccccctt cagggcgaca
    30301 aaaagattgc ctttttttat cgaacgggaa tctgtggaaa cgccgcgaaa aacagtttcc
    30361 ggcaatccgg acatcatcgt cccggacgtt gatgtcagca cgtcattcaa aaaaaataca
    30421 ggtgactcac tcttcattgt tcgtctcaat aactcttggt ttcaagaatc tgccgtgcaa
    30481 caacccggtc atcaaacgat atcttctccg tcccgagaat ctgataatct tcatggcctt
    30541 tgcctgcaat caaaacgata tcgtctctcc gggcggccct gatcgccgcc tcaatcgcct
    30601 ttctccgttc ggggatcact gcatatgaac gagcacgtcc gtttattttt aacatttccg
    30661 gatcgctttt tttaacgtct ttccggtcaa tgcccgtttc tatgtcagcg atgatatcca
    30721 gtggattttc caaacgcgga ttgtccgagg taatgatcgt caaatcgctg taccgggtgg
    30781 ccgcgtctcc catcaggggc cttttgcctt tgtctctgtt gccgccgcaa ccgaaaacgg
    30841 ttatgattct gtttttttta agctcggata aattttgaag aacacgccgc agcgcatcat
    30901 ccgtatgggc ataatcgaca aaaacgtgaa cccctccggc ggaaggcacc gattccaatc
    30961 tcccaggaac atgcgataaa ttttcgatcc ccgctttaat caccggcacg ggaatgtgta
    31021 aaataaaagc cgctccggca gcggctagaa tattgtacag attgaatttg ccgatgaggg
    31081 gtgattggat caagacattg tttcccgcca agcggatgtc cgcttctatg ccgttcagag
    31141 acagcttgca gtttaacgat cgtacggcac agtcgttttc tattccgtaa gtcagtgccg
    31201 gcaggggcac gtccttcaga attctccgtc cccagtcgtc atcggcgttg atgatcattt
    31261 tctgcgcata accttttctg ctttgcggaa ggatttccgc aaaaaatctt ttcttcgcct
    31321 gaaaataatt ttccatcgtg acgtgataat ccagatggtc gcgggtcaaa ttggtaaaaa
    31381 tacccagagc aaaatcacaa tcgtccaccc ttttcaaatc aacggcatgc gatgagacct
    31441 cggccacaac atgggtgacg ccgtcgtcgg ccatggcgcg gaggatcttc tgcatctcgt
    31501 aagattccgg cgtagtgttg ggagcatcat agaccttgtc gttatagcga taattgacgg
    31561 tgcccaggac accgcatttg catccggctt cttttaagat ggattccagc agataggaga
    31621 tggttgtttt gccgttggtg cccatcaccg caatcacggt cagcgccgcg gaaggatcac
    31681 cgaaataatt cttggccagg atccccagcg cccggcggct gtcggacacc ttgatcgctc
    31741 ttacgaatgg cgggaacgtc agaaccttct catgaacaat gaatctcgcg ccgcgggata
    31801 tggcttcctc aatgaaatcg tgcccgtcac gttttaatcc cggaatggca acaaacagag
    31861 actccggcac gcactggtcc gcagaaaaac aaacaccgga cacgtctccg gacgcctcac
    31921 ccgacatatt gatcatatcc aatccttccg tcaaccgagt cagtttcatg ccatcctcaa
    31981 tccgtcaatt caaaaaccac gctgcataca cgttcatggc ccagaggcgc gtgcggttcg
    32041 ggatactgac gcacggccca gccgttgccc gatattttca gctcgatgga ttcggttttt
    32101 gcttttttca gcgcttccct gatcgttaat cccataaaat tgggcatgga cggtccgtcg
    32161 ctgttctcga caatttcagg gagcaaggtt tgttccgccg acaccaactg aaccttatcg
    32221 gttttttcct tctcaaaaac gggggtttct ctgatatcgg ttttaaaaca gttcaggatc
    32281 tgttccgcta tatttttgaa tacgggcgcc gacgcaacgc caccccattt gtctttttgc
    32341 ggctcatcga gaaccaccag tatggcaacc tgaggatctt cagccgggaa aaatcccata
    32401 aacgatgtac gaactttttc cgatgaatag acgccgcggg cggcgtcgaa tttctgggat
    32461 gttcctgttt tgccggctac cgctacattg acaatcttcg catttttacc ggtgccgtct
    32521 tcggcaccga cgacatccat cagcatattc gtcacgctct tggccactct gggagaaatg
    32581 acctgacgca ccacggtggg tgtatagagt ttgaccaacc ggttatcctg gtcggcaatg
    32641 ccgcgcacga tatacggttt catcaaaacg ccgttgttcg cgattgcgga gacggcactg
    32701 atcaactgaa tggccgtaac ggaaataccc tgaccgaaag caatcatgga tgtatcaact
    32761 tttacccagt ccctcaccgg ccgcaccagt ccggaagatt cccccggcaa atcgattccg
    32821 gttttggatc cgaaaccgaa tttttgaata taattataaa atttttcttt gcccattttc
    32881 tgggcaatct ttgcggaacc gatgttactg gaatatttta agatctccct gaccgttaat
    32941 gtgccgtaac gttttctgtt ggcctcatga atcacgcgat cggcaatcct gtaattgccg
    33001 ttttcacaaa aaaatctgtc ctgttcgcga ataacttttt cttccagagc ggcggccacc
    33061 aaaaacggtt taaacgtgga ccccggatca taataatcgg taatggcgcg attccgccat
    33121 cgctcgggag acagtccctg gatgttgttc ggattaaacc cgatctgatt ggccagcgcc
    33181 agaatctctc ccgtcttcgg atccatgaca atagccagcc ctcccttggc gcctttttca
    33241 atcaccgcct cttttaaatg ggtctcaaca agatattgga ttcggttgtc gatcgtgagg
    33301 acaaggttgg tattttcatt ttttgcggtc tgcgtttttt ccacccggag gaaaagtttc
    33361 ttccctttgg catccctcgc ccattcaagt ttttccgggc tgcccactaa agagtcattg
    33421 tatttctttt ccaacccttc cagaccggaa gcatccaatc cgacaaaacc cagcagatgg
    33481 gcggccaact ctccattggg ataaaaccgt ttcggctcct tgaccagaaa aacgccctcg
    33541 acatccgctt tcaccactgc agcggtttgc tcaggcgaaa tccgcctggc cagccagcag
    33601 aagtttctgg gggcggataa ttttttatac accgcctgac tgtccaaccc gaggatgtac
    33661 gcaatctgtt tggatgcctt ggccggatca atgactttcg acggatcggc acagacggaa
    33721 tcggccataa tggaaatggc caatttttca ccgttgcggt cgtaaataat tccccgttcc
    33781 ggctgcaact gcaaaaccgt cgtatgctgc cttgttgcca gtttcttcag cttttgaccg
    33841 gataaaacct gaagctgaaa cgcgcgcgat atgagcgcca caaataaaac gaaaaaaaac
    33901 atgaaaatca tgatgattct gaatttcatt ctttttttcg attcaatcgc catgtcagcc
    33961 tcccgatttt cttataacaa tgacctgttc attgtcaggg taggacatct gcaatctgtc
    34021 gcggacgaga ttttctattc gttgcggcga tttgagcatg gcatattcca gtttcaattt
    34081 tttctgttct tccagaattt tctcctgggt actgatttcc gctgcgatat tgtattccag
    34141 ttcggtcatg cgaatatgcg agccgacata agcaacaaac acaagcatca gcacaacggc
    34201 gagaacaata aacgtcggat atttaattcc gacggacgct ccttcgactt ctcccagatg
    34261 ggggacaact cgtgcgtctg ctgattgagc ggatggggcc atcttacaat ctcccggcta
    34321 ctcttaattt agcgcttctc gcccgcggat tggacgctat ttcttcctcc gaaggcatga
    34381 tcgctttttt tgtcagattt ttaagtttgg catctctctg acagacacaa aacggactgt
    34441 cgggggggca tacgcaacgc ccttccagcg aacgaatctc atttttgaca atacggtctt
    34501 ccagagaatg aaaagagatc atgcaaagac ggccgccggt ctttaacaca tcaactgccg
    34561 catgcatggc cggtttgatg ttgtccaact cgtgattcac ggcaattctg atcgcctgga
    34621 acgttctggt cgcgggatga attttgcgcc atcgcgttcc ggcgggcatg caggatgcga
    34681 caatcgccgc cagctgtgtc gttgttgcaa tcggcgattc ctgtcttttt tttgatattg
    34741 ccctggctat tcttgaagcc attttttctt ctccgtaaaa cctaataatt ttttccagtt
    34801 cgttttgcgc gaagctgttg acaatatcat aagcgctcaa cgtcatgctg cgatccatgc
    34861 gcatatcgag cggcgcctgc tgactgaagc tgaaacctcg ttcagatgca tccagttgac
    34921 gggatgaaac acccagatcc agaagcacac cgtcaacttt ttctatgttt aaacttttca
    34981 gtatatggtc taagttaaca aaattttctt ttagtaaaat tttgcgtgag ccgaattctt
    35041 gcagtctttt ttcggcaaac cgcagagctt catcatcgca atcaatgccg atcagcaaag
    35101 catccgtctg cttgaggatc gcgtatgaat ggccagcccc tccaacggtg ccatccacat
    35161 aaaccccggc cctgctgacg ataagcgaat ctataacttc cttcaccatg accggctggt
    35221 gataaaactc ggccgtcatg gtttaaattc ccagatcagc tataaagtcg gcgcatttgt
    35281 caatttctcc gacagcctgc ttcatccttt cttcgaaacg gtccttcgac cagatttcaa
    35341 tatttttcag catgccggcc agaacaatat ctttttccaa ttgagcatat tcgcgcaaag
    35401 acggcggaat cagcacacgc ccctgtccgt caatactgca atcaatcgct ccggacataa
    35461 agaaacgctg aaacgatctg acttccttgc gcaacttgga ttgatgggcg accttctctt
    35521 caataaccag ccattcatcg taaggaaaga ccatgagata ctgatcccag ttggtaatca
    35581 ccatccggtt gtcatactct tcagcaaaga tttcacgaaa ctttgacgga aagattatcc
    35641 ttcccttttc atcaattgta tggtgatact gccccctgaa aacagacatc acttccctcc
    35701 acgataagcc actttactcc acgtggcaac tatagaggcg ctatttgtct ttgtcaagta
    35761 aaaaatgcaa ctttttttta aattaaatag cttaaaatta ctgaagaaat agggaaattc
    35821 tgataaaaaa atattttatg gcgagaaaaa caaaaaaaca ggaaattttt aaacaaaggc
    35881 tggatttttt attttaatac tcctcaacgg gaatatcata ttcaagtaat tgattttgtt
    35941 catcttttaa attaatatac cgattaaccg cctgattatg ttccaccaat gaactggaaa
    36001 aatggtgagt gccgtctttt ttggaaacaa aataaagata atcgacagag ctcggggaaa
    36061 gcgttgcctt tagcgaatcc aggccgggat tggcaatcgg ccccggaggt aaccctttga
    36121 tccgataggt attatatgga gaattcctct tcaggtgact acgaaaaatt ttaccctcaa
    36181 agtcatccat gtcataaacg gccgtcggat cgctttgcaa aggcattcct cttttcaacc
    36241 tgttgtaaaa tacagcggca acggaaggtt tctcgagagc actaccggac tctttgccga
    36301 tgatggaggc catggtcact aatttatgca cgcttaatcc catctgattc gccctccgaa
    36361 tcatggacgg cgtcattttc tgccagaatg tatcgaccat tttcttcatg atgcgacgtg
    36421 tattcatcga acggttaaaa tgatacgtat caggaaacag atacccttcg atagactccg
    36481 cttcaatatt caacgatttt aagaattcct gatccctggc cagctcaaaa aatatttctt
    36541 tattaatcag cttatgcttg tccagaatca ccgcgatgtc gcgaaatgac aaatcttccg
    36601 gaatcacaac ctcatagact tttatatcac cgtgcatgag tttgttaatc attgttatgg
    36661 ggttcgtcaa cgtattgatt tcatactctc cggcacgaat gttacgactg gccctcttga
    36721 tcgcggccag gctgtaaaag aaaaaacggt ttgtgattaa tccttctttc tgaagcaatt
    36781 cagtcgcttc catgaagctg gaacctgtcg ggatatcaat cacaaccgtt ttatctcgga
    36841 catcgatcgg actgaatgca taatccaata acataataca gaatatgagg atgacggcaa
    36901 cactgtataa aacagtgcgg cgaatccatc ttcttccgga aaatattttt ttcagaaaaa
    36961 tgaagatttc atagaattta cttttccaag caattgcccg tgacgtcatt tgatcgaccg
    37021 cgttgttttc ttcatttgac tttttcagga tattcttttt gtcaggattt ttcatggctg
    37081 ttcagaaatt atttttttaa tgatggagcg acagaatcca gataactttg aagaataaga
    37141 caggccgcca gtttatcgac catttttttt ctttttcccc accggatacc ggagttgatc
    37201 agtatttctt cggcttcctt ggtggacagc gtttccggcc acctgatcac ctgcaaaccg
    37261 aaggtttttt ccagcagcgc ggcaaaacga ttaacttttt cacactggat gccttccgaa
    37321 ccgtccagct tcaggggata cccgatgacg attttctcaa cgttaaaatc cctgatgagc
    37381 ctttctaaga tttccccgtc atgctttttt gtttttcgta tgattgtgga caatccctgg
    37441 gctgtcaacc cgagttcatc acagatggcc gcccctattc gtttttcgcc gtaatcaaga
    37501 ccaagtatac gcaaaaaata cctctttatt tttttctaac aattccatcc ggcattttca
    37561 agaattaatt tgtccagttt ttttcaacat gggtttttta tttttgatgt cttgaaatat
    37621 aaccttgtta aaaaatgaag agtttgttat ttttcgcttc cagataatca ggattaaaaa
    37681 atgaagcatt ttgttttggg caccgccggt cacgtcgatc acgggaaaac ggctttaatc
    37741 aaagctctta caggaattga cacagaccgt ctcaaagagg aaaaacaaag gggcatcacc
    37801 atcgaactcg gttttgcttc cctgaaattg ccttcgggtc tgacactcgg gatagtggat
    37861 gttcccggac acgaaaagtt tgtgaaacac atggtttccg gcgctgccgg cattgatctg
    37921 gttttaatgg tcattgccgc agatgaaggc atcatgcctc aaacaagaga acatctgcac
    37981 atctgttccc ttctgggaat ctccagggga atcgtagccc tgaccaagat tgatctcgtg
    38041 gaaaaagact ggcttaatct tgtcgagtct gaaatcaccg attttcttcg aggaacattt
    38101 ctggaaagcg caccggtcat tcccgtttcc gccataaaac aggaggggtt gtccgagcta
    38161 cttcaggccc tggataccat catcgcagag attaccgcaa gaacggacga cggtattttc
    38221 cgtctgcccg tggatcgcat ttttaccatg aagggtttcg gcacaatcgt caccggtacc
    38281 ctggtttccg atcaccttaa aacaggggaa gacgtacaga ttcttcccca gaatatttcc
    38341 gcccgcatca gaggcattca ggtacataac cggcctgagg aagaagcctg ggccggacag
    38401 cgtacagcgg tcaacctgca aggtattgaa aaatccgcca tcgtgcgagg caatgttctg
    38461 gtgcggccgc acaccgtctg gcctacgcag cggctggata tcgccgtcga ctatctttcc
    38521 gccaatacca aaagtcttaa aaaccgcgcg ctggttcggc tgcacaccgg cacgaacgaa
    38581 atcatttccc ggattattct tctggataca gatgaacttg cccccgggca gcaaggatac
    38641 gcccaactca ttctttctga aaaagacgtt gttgtcgccg gcgatcattt tgtcttgcgc
    38701 agttattccc cggttaccac catcggcggc ggcgtcatta tcgatccgct tccgcaaaaa
    38761 cataaacggt tcaaccgcgg agcgatcgaa gaactgatgt tgctgcaaaa aggcacctta
    38821 ccggaaaaaa ttaccgtcat tctggacagg accgggttta aaggaacccc gcttcaacaa
    38881 ttggccttcc gtctgggaat gaaatccaga aaaatacgag aggttctgga cgatttattt
    38941 tccaaaaagg aggcgtttct gcttgatagc gaggacacga cggttatttc cgcacgtttt
    39001 ttcaatcgac tcgaagagat gatcatttat aatctcagtg ggtatcacaa gaaaaatccc
    39061 ctgaaagaag gcattcccaa agaagagctg aagggatccc ttgccaatga tatttcaccg
    39121 aaactcttta acatggttct caacacgctc aacaaaaaag agacgatcgt cagcaacaaa
    39181 gataatgtgc gtctggccaa acatcaggtg gagcttgcgg gtgatctgga tgctctgcgt
    39241 cagaatatta tcggaatata caaaacagca ggactgatgc ctccttcgct gacagacgtc
    39301 atcaacaaat ttattgacag gaaaaacaaa gcacaaaaca ttgtcagcct gatgctcaaa
    39361 gaaggagaat tgatcaagat caatgaagaa ctatgctttt ccggcgaaat cctgagcaaa
    39421 ctgcgcaatg attataaggc aatgctgatt aaagacggca aggcaacacc ggccagtttt
    39481 aaggaactga caggtctttc ccgcaaatat atcattccac taatggaata ctttgacatg
    39541 aataaactga cggttcgggt aggagatcac cgcgtattaa gggaaaaaac atgaacgatt
    39601 ttcaaaaaat catcaagacg ataagatgct tttccggcag ctattctgcc gtgtccggat
    39661 cccgcgtcaa agtgattcag gaagtgatga tcaccgtccg ggtcaacaaa acccctctgg
    39721 ccaatattgc ctgcgccggc attcatctcg aagaactcgc cgccggattc ttacgatcgg
    39781 aaaagatcat cacccgccgg caggacattg aaaacatctc ccttgccaaa aaccgggttg
    39841 acgttctttt gaagaacaaa aatttttttc cggacgtgtc cgtgaaaaat attgcatcga
    39901 gcggcgcacg cggccgaagc aacgtcgctc tgctgacgcc gctgaacgtc ccggccggct
    39961 tgagtatcaa cacagcgctg gcactgaaac ttatggacga tcttttaaac ggttcggcga
    40021 tgcataatga aactggcggc acgcactgct cggccctggc ctttagaaaa aaaattgcgg
    40081 ctctgcgcga agatatcggc cggcacaata ccatcgacat gctgggcgga tatgctctgc
    40141 tgaataaact caatctccat cgggccatca tattgaccac gggcagaatt tcttcggaaa
    40201 tagtctataa gatatggaac atgggcgtac cgttcatcat ctctcattcc gcaccaacgg
    40261 ccaatgccgt gaccctggca aaacaggcca atataacgct gatcggctat gtcaggaaag
    40321 gacggatgag catatactct cacgaaaggc gggtcatcat ttgaaccata aaaacgttta
    40381 tctcaaggat atcaaacagg gcgataaagt tgcttcttct tttctggtaa cggaaaaaaa
    40441 catggccttt tcacaaaaag gctcccccta tctgacggtg aggctgaaag ataaaaccgg
    40501 cgaaatggaa tgtaaagtat gggaaaacgc cttggaattc gataacgtct tcaaaaaagg
    40561 cgatgtcatt tttatcgaag gacgggccgc gagctacaaa aactcgctgc aaatatccat
    40621 tctcaacatc aagaacattc cccggcaaga tattgatcct gccgattatc tgcctgcggt
    40681 aaagaccgac gtcaaaacaa tgtttgcgga actgctggcc tttgcggaaa aagtaaacca
    40741 taggcatctc aaggcattgc tgaatgcttt tctgcaggaa gaagaaactg ccgcccgttt
    40801 tcagcgagcg cccgcggcca aaggattcca ccatacctac ctgggaggtc ttctggaaca
    40861 tacgctttcg gtggtccgcc tgctggataa agttgccgat cattattcca tcctcgacag
    40921 ggatatgctg atcaccggag gtattcttca tgacatcggt aaaatttacg aattctcttt
    40981 cgagagcatt attgactaca gcgacgaagg aagattgatc ggccacatcg ttatgggtgt
    41041 ggaaatgatc gatcagaaaa tcgcggcaat caacggtttt ccgccgcagc tggcaatgga
    41101 aatgcgacac atcattctca gccaccacgg cgaattcgaa ttcggttctc ccaagcgtcc
    41161 gaagactctg gaagcgctcg tcgtccatta tatcgacgat ctggacgcca aattcaatgc
    41221 ttttcagacc ttcatgaacg agtcctccag tcccgattcc gattggaccg tgtatcatcg
    41281 ttttttagag cgttttcttt ataaaaagaa aaacgccgcc ttcgatattc ctgaagatcc
    41341 gaaaatttaa acttgatttt tgactaaaaa aaagcgtatg aaaacgcact gcaaatcaaa
    41401 aattaataaa caagaaaact atctaccaac accatcacga tagttcatta aatactttac
    41461 aggggggagg atgtagttat ggcatacagg acggatactt tatgcggcat ttttcacaat
    41521 caggcattgc gttacggtga tcaggttgaa ctgcttcggg ccaagtttga tcaaaacggc
    41581 aatcccagcg ctcaatggca ttcccggaca tggaaagaga cccgggagga agccatcggc
    41641 ctcgccaagg ggctcatggt cctggggcta aaaaaaagcg accgtatcgt tattttttcg
    41701 gaaagcagac ccagatggat tattgccgat caggccattc aggcctgttg cgccatcggc
    41761 gttccccttt atccgacgtt aaccgtggaa gagctgggct acatgacatc ggacagcgga
    41821 tccaaaattg tcattgcgtc cacacagggc cgcggagaga tggctctcaa agcaaaagca
    41881 cagggggttc ccattgaaaa aatcatcgtc atgtgctcct gggaaggcgc aaaacccgaa
    41941 ggggtttaca cgtttgatga agtcatcgac ttaggcaaag ggaaaatcag cgacgaagaa
    42001 atagaaaaaa atatcaacag cgtcactccg gaagacgtca cttccatcat ttatacatcg
    42061 ggcacaacgg gaaaacagaa aggcgccatt ctgacccaga aaaactgggt atccaacatg
    42121 cttcaggctt ccaattccac gctgatgcgg aaacagcgtg aactgggact tcacctgact
    42181 ttcctggttc acctgccgat gtgtcacgtc ttcggccgga caagcgatta tcatgtgggt
    42241 gctctgcagc aaagcgggat tctggttttt gctgaaagct ttgaaaaaat cccaaagaat
    42301 attctcgaag tcagaccgaa tgttttaatc agcattcccc gattatggga aaagatttat
    42361 gatacgaccc ggtcgattgt gtcccgtcag gataaacggg tccaatccat tttcaactgg
    42421 gccacccggc tgggtgaaaa atactgcgaa gcgatggcta cagcgaccag actgggtttc
    42481 ttggatctgc tccagttcgc gctggcgaac attctggttt tcaataaaat caaaaaaaca
    42541 gccggattgg atcatgttgt tctcggcata tcgggtggcg gcaagctatc caaagaagtt
    42601 tgcgttttta tccgcgctct gggcattcag ctggccgaag gttacggact gaccgaaacg
    42661 tcgcccgtca tcaatttcaa cgaaccggaa ttcgccacca gctttgatat cagcaaggcc
    42721 agttggtttc aaaaaaagat gcttgattta acggttgatt tgatggtcac caagcaggcc
    42781 aaagggcttt ctccctatac caatcctctc cgctccttga gtctttctct ggcatacaac
    42841 acgctcgccc acaaattgca gattaaaccc ggcaccgtcg gaagaccggc gttgtggacg
    42901 gaagaaaaaa tcgcagagga cggcgaaatt cttgtcaagg gacctcaggt cttccaaggt
    42961 tattggaatc tgccggacgc aaccaaggaa gctttcacgg atgacggatg gttcaaaacg
    43021 ggcgacatcg gagaatttga caaggacgga tttcttacaa ttaccgaccg caaaaaggaa
    43081 ctgtttgtca catcaggagg caagaatatt gcacctcatc cgatcgaact ggcccttacc
    43141 gccaaaccgt atatcgatca ggtctgtttg atcggagatg cgagaaaata catttccgca
    43201 ctggtcgttc ccgattttca ggaactgcat cgtttttcca aacacaacgg catcccggcg
    43261 tgttccgata aagacctgct tgccaacgaa caggtcaaag aactgattca gaaacaggtt
    43321 gatgaagtca acgaagcact gccccgctac gagcagatca aatattacaa actactcgat
    43381 aagcccttca gcgtcgaaac gggcgaattg actcccacgc tgaagttaaa acgcagaatc
    43441 gtttttgaaa agttcaagga cgatattgaa agcatgtaca aagattaaca tcgcggaagt
    43501 cttaaaaaaa gacatagggg gacggtcatc gtggaccgtc cccctatttt ttggtcatta
    43561 tgtcaatggg ggtgactctt tatctgccct tgaaatccgg ctgtcttttt tccatgaaag
    43621 caacaaaggc ttctttcagg tcatctgtgg cgagcaggaa catgtttttt tgaatggcca
    43681 gagacattcc ttcgctgacg ctcgtacctc tgctgaaatt aagaacctcc ttcgtgcatt
    43741 caatagccag cggagcgttc tctgtgattt gtcgagccat ttcacgggcc gcctcataaa
    43801 gcgccgcctg atcagcatag acatgattga ccagccccat tttctcaacc tgctgggcat
    43861 tataaaaggc aacggtgaac gcgatttctc tggcatagcc ctgccccacg atcagaggca
    43921 atcgttgaag cgcgcccatg tcggcgcaca agccgatttt cggttccttc aggcagaacg
    43981 tcgcatcctg agtacagagg cggatatcgc aggcggaaat aatgtgaagt ccggctccaa
    44041 tgcactgacc atgcaccgcg gcaatgaccg gtttgggaca ttcttcaatg acattgcagc
    44101 aatcaaacaa cggctgtgat tttttaatca tatcccatgg cgctctggtt gttcccgtaa
    44161 aaccaccggc ggcatccttc aaatccagac cggcacaaaa aattctggcc ctgcttttca
    44221 aaatgacaac tctgacgtca tccatggcac ttaattttct aaacacatcc gtaatttccg
    44281 aggcaagttc caggctaacg gcatttaagc ttttttctct ggccagggtt acggttgcga
    44341 tatgatcctt gacctccagt tcaataaatt tataattcat atttttctcc ttaaaaaaat
    44401 tttacacgtt caattgtccc ggcactcctt gtacaaatta tgcttcatga tgtattgacg
    44461 gaccttgccg ggtgtaagat atcggatgga attgccggtt ctgaccatgt ctctcatgcg
    44521 cgacgatgaa atatccagga aactcgtatg gcggaaatag atggcctgtc ccgttggacc
    44581 tttaaatccg tcggtttttt tatcgtagat gaattttgcg gcaaaggttt tcggcagaat
    44641 tgcatcaagc ctcatatcat cataaccggg acgggtcatc acggcaaaat tacataatag
    44701 caaaagcctt tcccaatcct tccaggttgt caccgcctga aacgcatcct gcccgacaat
    44761 aaaataaagt tccagatctt tcatgtattt attaagaatg tattcgactg tttccacgga
    44821 ataggattta ccggcgcgca gattttccac ctcggaaaaa gagaaattga cattgtcttc
    44881 aatagccaaa cgaatcatct gctcgcgatg attgaacgcc gtgatatccg cttccagctt
    44941 gtgcggcggt ctggaggatg gaacaaagat gatacgattc agattgaaaa tttcgagcat
    45001 ttcctccgcc ccgcgcaagt ggccgaaatg aatcggatcg aaagtgccgc cgaaaagtcc
    45061 ccatctcatg ttctcacctg tccgttcccg taaatgataa attttgttgt cgtcagttct
    45121 tccaagccca tggggccgaa agcatgaagt tttgtcgtcg agattccgat ttcggcgccc
    45181 agtcccagtt caaagccgtc gctgaaacgg gtcgacgcat tcactaggac ggtcgaagaa
    45241 ttgacatcgt ttaaaaagcg ctgggcattg gaataatcac ccgtaatgat cgattcggta
    45301 tgcagagagc cgtatttttc aatatgggca atggcttcat ccatgttttc caccaccttc
    45361 acagccaaaa ccaagtccag atattcggca gaccagtcat cttcaaccgc cttctcgatt
    45421 cgtttcaaaa tcgattttgt tttttcgcat ccccggatga cgacgccggc ttttttcatt
    45481 ttattggcaa ccacgggaag aaatttccct gcgatcgtct gatgcacaag caacgtttcc
    45541 atcgcgttgc acacgccggg gcgctgtgtc tttgcgttca aacaaatatc aacggccatg
    45601 gccatttcgg cgctggcatc cacaaaaata tgacagaccc ccttatagtg cttgatgaca
    45661 gggattcttg actgggcaac caccgcgcgg atcagatctt ccccgccgcg gggaatgatt
    45721 aaatcgatgt attcttccat tttcagcatg gcagccaccg cttcgcggtc cgtcaccgga
    45781 atgacctgaa tcgcttccac gggaatggat gtatcctgta atactttccg caaaacggat
    45841 gcaatcgcca gattggattt gatcgactcg gaaccgccgc gtaaaatgac ggcattgccc
    45901 gctttcaggc agagcgctga agcgtccacc gtaacgttcg gccgcgattc gtaaataatg
    45961 ccgatcactc ccaggggaat gcgcattttt cccaccagca aaccattggg ccggcgccac
    46021 atggaaatga ttttaccgac aggatcgggc agcgtagcaa cttccctcaa tcctttggcc
    46081 atttgatcga tcgttgattg tttaagggtc agacgttcca gcatggcttt ggagatgcct
    46141 gcttttttcg cgccgtttac atctttggcg ttttcatcca gaaggaattt tgaattccgt
    46201 tccaaggctt ccgccatgcg taaaagagcc gtgttttttt ccgtttcagt cacacgggaa
    46261 agtttttttg cggccagcgc cgcattccgg gcaatgtgct caacagaatc ttttatatcc
    46321 atgataatca tcctcacgtg aaatgaattt ccggattccg gaagcggaac cggaaatgtt
    46381 gtccaataac aaacggtatt ttttacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46501 nnnnnnaaat tatgaaccct cttctcacat aagaattcat aagagctgta agtgaatcta
    46561 caagacaaaa gtttacgtgt caaggccttt gcccgggcat aagatctatg agtagaatac
    46621 gaattaaagc cggcaggaaa atttatcaac ttatcaaaga cggcgggttc aactttgatc
    46681 tgatttcgac gtatttcgga ccggcggtgg gaccgcgctg gctgattgcc agcggcttcg
    46741 atttgacact tcttgaaaga tgttccctgg gccgttcgac ccctgtccaa ctcgtgggct
    46801 cttcagcggg cgccttccgt tttgccgcct ggctgcaacc ggaagccgtc aaatcctacc
    46861 ggcaactgat ggaggcttac attcatgtta cctacacaaa aaaagataca ccggcaacga
    46921 cgctggaaaa aataaccggc atcatcaatg cgtatctgga aaacgacgct ctgccttttg
    46981 ccctgaacaa caagaaatac cggctgacca tcattacagc gcgttcacgc ggccttatcg
    47041 cttttcaaaa cgtcttattg cagaaaacag gattgatttt ctgttttcta ttgaactatt
    47101 taagtagaga aaatatttac aaatttgccg aaagagtcgt tttttacaat gcgtccaaac
    47161 cgccggcttt ttgcctgaaa ccgcaattcc gcggcatcta tgtcccgctg aacgaaatca
    47221 atttcaaata cgctgtgctg gcttcgggcg caatcccgct ggtgattgaa ggtgtccgcg
    47281 acatttttgg cgctccccgc ggcgtgtacc gcgatggcgg cctcatcgat tatcatctga
    47341 cccatccgtt tgcgtccgga gaaaacgaca tcgtgctttt ttttcatcat caggaaagga
    47401 tcattccggg atggctggac aaaaaaataa cgcgccgtgc gcccgatccc aatatactga
    47461 ataatgtttt aatggtcttt ccttctcaat attttacgga aagccttccc ggcggaaaag
    47521 tccccgaccg cacggatctg gtcacattca ttgacgaccc gcaaacaaga atcagaaatt
    47581 ggtgcaaagc ggtggacctg tccgctccgc tgggcgacga ctttctggaa ctggtagaaa
    47641 gcggcagact ccgcgatgtg gtggaaccct tttaaccaaa ggatgctccg gacggaattt
    47701 ttaagaatga atttggaaga aaaaattttc ctcctgcagg aaattttcaa agactgtatc
    47761 ctttgtccgc gaaactgtca tgtgaaccgg acgaaaaacg aacggggata ttgcggtctt
    47821 ggggccagaa ttatcatgga ttgcgcgctg gcgcatcacg gcgaagaacc gccgctctcc
    47881 ggtacgaacg gcgcgggaac gatttttctt tcctcctgca acctgggctg catttattgt
    47941 cagaatcacc agatttccca ccacatcaca gggcaggaaa tgaattcaag gcaactggcc
    48001 gatgtgatgc tcgatctgca gaaacggcaa tgccacaatg tagaaccggt cacgcccacg
    48061 tcacagatgc cgctggtgat ggaggcgctg ttgattgcac gcaaggcagg attgaaaatt
    48121 ccttttgtgt ataattgcgg tggttatgaa agcccggaca tcctgaaacg gctggacggc
    48181 atggtggaca tttatctgcc ggatttcaaa tacggccagg aggaagacag catgatgttt
    48241 tccgccgccc ccggatatcc gtggtttgcc gcggactcga taaaagaaat ggtgcgccag
    48301 gtcggcgacg cgttggaatt aaaaaacggg atagcgcgga aaggcatcat cattcgtcat
    48361 ctggttctgc ccgggcgcat cgagaattcc aaagcagttt tgagattgat cagcagaaaa
    48421 atatccaaca atgttttttt aagcctgatg tctcaatata cgcccatggc caaggtccgc
    48481 aatcatccgg atttaaaacg ccgaattaca aaaaaagagt acgaacaggt tttggactat
    48541 gccgagaaat tgggttttca taacctgttt attcaagaag tcagcgactt cgacctgaca
    48601 ccggatttta gcctggataa tccattcgaa tcctgaaaag aatttatgga cattgatata
    48661 cgtgaaattt acgatcgttt tgcccaaact tacgacaaaa accgttacct gttcgatatg
    48721 acggatgtca tggacgattt cttcagaaga ctgccttccg tcaaaggcag tttgctcgat
    48781 ctgggttgcg gtgcgggtga accgtttccc gcctatttca ttcaaaagga atggcaggtt
    48841 accggagtgg acttttcatc aaaaatgctg gaattggccc accgttacca accggcaatg
    48901 aagaccatcg ccgcagacat ccgggaggtg gagtttcccg gcgaacaatt tgatgcggta
    48961 acaaccattt attgtctctt ccacattgaa tacgaaaagc acgaaggcat tttccggaaa
    49021 atttaccgct ggcttaaacc gggcggcagg ggacttttta cctacgccac aaaagcgtat
    49081 accggag
//
LOCUS       DAONQZ010000013        46555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_848_length_46555_cov_18.223828, whole genome shotgun sequence.
ACCESSION   DAONQZ010000013 DAONQZ010000000
VERSION     DAONQZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 46555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46555
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_848_length_46555_cov_18.223828"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..1658
                     /locus_tag="PLR13_03940"
     CDS             294..1658
                     /locus_tag="PLR13_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQC18515.1"
                     /translation="MEQVKVVLQDREIPKKWYNIMADLPTPMKPPLHPGTGQPVTPQD
                     LAAIFPMNIIEHEASNKRWIKIPEEVLQKYLLWRPSPLYRAYNFEKLLNCPVKIYYKN
                     EGVSPAGSHKPNAAVPMAYYNKVAGTKRITTETGAGQWGSALAMATQMFGLECRVYMV
                     KISYEQKPYRRLMMNTWGAECIPSPSTLTQSGRDFLAKYPDSPGSLGIAISEAIEDAV
                     TSKIKTHYALGSVLNHVLIYQSIIGLEAEKQMQKLGIYPDVVMGCCGGGSNFAGIAAP
                     FVRDKIHGKDVKIVGAEPSSCPTMTKGPFAYDFGDLAQKTPLLPMYTLGHDYVPAPIH
                     AGGLRYHGMAPIISHLVKEKLIEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAV
                     VDEAMRAKKEGKEKTILFNWSGHGIIDLAAYDAYFAGKLKAYELPGHEIERALKAIKN
                     FPKP"
     gene            1794..1997
                     /locus_tag="PLR13_03945"
     CDS             1794..1997
                     /locus_tag="PLR13_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18516.1"
                     /translation="MTFAYCLREGGNLPCVRIIRCWSPVFDIESFLKGHLSEKRWLKF
                     INTKAPDKITSLIELIEAAKAKK"
     gene            1994..3343
                     /locus_tag="PLR13_03950"
     CDS             1994..3343
                     /locus_tag="PLR13_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQC18517.1"
                     /translation="MKTLDLFDQGNSGNVKDAAPLAERMRPQNIYEYIGQKHLLGEGS
                     LLKRAIESDKLFSMIFWGPPGSGKTTLARILANETKSKFVSFSAVLSGVKEIRAVIDE
                     ARELLERKSIKTILFVDEIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEVIAPLL
                     SRTRVLVLKPFSEENLLAILEAALKNKQKGLGNIDVKIDANVLRYLVSLADGDARSAL
                     NNLEVLVSMVQNLPDDQRAISLKFAQDALLKKSLLYDKDGEEHYNLISALHKSLRGSD
                     PDAALYWLGRMLAAGEDPLYIARRMIRFASEDIGNADPQALVVAVAAQQAFHFIGLPE
                     GELALAQAAVYLATAPKSNALYVGYGKVKNTINTKGYLPVPLHIRNAPTKLMKDLNYG
                     KDYKYAHDYEESYVPQDYLPEEIKGELFYQPKGVGFEKTIKERLNYWRNKKKEGEKE"
     gene            4086..5873
                     /locus_tag="PLR13_03955"
     CDS             4086..5873
                     /locus_tag="PLR13_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18518.1"
                     /translation="MRLFRKKDIVVHRKAHNYNDPFLQPLAVAIVSAIFVALILITGF
                     LELRRSESNLIAFMENQALSTIGVLQRLTEENLKSIIGVPDKNAPHEKTSGRDEASYS
                     KQWVIQAITELAKDLDEQWRKGDMTDESLRQFASDNNFSYVGMLNAQGNAVFQSGPLQ
                     RDLLEENELTHKGRKLTTLELISKIGEKQGVGFIALSRKDNTGAVIISLDKSGSVYWI
                     LKIAGERGMDKIGEGHGIVYMQIFNDRDKLLSSVGQPAPALNHRIFNLKEILSGKIKI
                     VSRKVDYADNKILDLAAPLYLDKKIVGIVRIGLDRNSMDKSLVENRQHIFVFSIFIVL
                     IALLSMWLLYHDQNRHLAGIVEMERQLEKAERLSSLGQLAAGVAHEIRNPLNAISMAT
                     QRLKRDFVPSDPQKVDDFQNLSGIIRDEIKRLNGIIEEFLNFSKSRRLDFCKFSITEL
                     LQKIVSLMKEEALLRGITLETTWKQESVPISMDVNKLQQAFINLIKNAMESITAEKGR
                     IVISVDKEGKNYIVVKISDSGCGMTEEELEKIFNPEYTTKEKGVGLGIPLAFEIIRGH
                     GGDIRVTSRKNKGTTFEVILPRERLNEVS"
     gene            5895..7259
                     /locus_tag="PLR13_03960"
     CDS             5895..7259
                     /locus_tag="PLR13_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC18519.1"
                     /translation="MQKLSILIVDDEKSQRELLRDFLRSEGHTVTEADNGEKAIKMVL
                     GGHFDLILLDYKMPGMNGLEVLKEVKRINPEIDVVIITAYGTVETAVDALKAGAIDYI
                     TKPIVELDELIILVNRIAERRQLIQENRLLKDDLSKHGITADKIIYQSRQMAELINLA
                     GRVAPSKASVLIQGESGTGKELLARLIHQLSPRADKPIIIVNCVALHENLLESELFGH
                     EKGAFTGASSRRIGRFEEADGGTIFLDEIGELSPTIQVKLLRFLQEREFQRLGSNVNL
                     QVDVRFISATNRDLEIQVAEGTFRDDLFYRLKVVTMSLPPLRERKEDLPVLIDYFIDK
                     FAKENGKNIKGFTAEARDLLLKYDYPGNVRELENILERAVVIAREQYITTNDLPFKSD
                     FLLEDSEKKPFGALKDSLDELEKKLIIEAMEKTQNNQTRAADLLGISERMLRYKLKKY
                     GLKG"
     gene            7313..8038
                     /locus_tag="PLR13_03965"
     CDS             7313..8038
                     /locus_tag="PLR13_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18520.1"
                     /translation="MKPKLIFLYIFVPIFFLVMAHILGQLAIFNIIPCFRGYDLFSVF
                     FNLDGEANIPTLYTILEWFLCVIFLVLIAFFERNTQSSRYRYWLGLAIIFSFLLIDEF
                     ISFHEISDHILALFNIKIVLFPTNAWVLIYLVVLAPISLVYLRFMIHLPKKTKYLFVF
                     SFIIFMLGAAGIEALELRHVEIYGQDKYYYLYFVIVEETLEMMGLLIFIYALASYLAN
                     ELGISQIKILPEKISHESTDKSA"
     gene            complement(8314..8793)
                     /locus_tag="PLR13_03970"
     CDS             complement(8314..8793)
                     /locus_tag="PLR13_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQC18521.1"
                     /translation="MSVFAASDIVEVAIRIEENGVNFYKFAEQIAKKEDEKKLFAGLA
                     QAEVAHKKTFEKLFAGMEKSNLQESYEGEFGEYLRAYVDNNLIFTKEIMDKQLAKVTD
                     TTSALEFAIQRELDSILYYHEIKKMVPAAQHDALEKIIEEERKHFQALTAMKKRLAC"
     gene            complement(8865..9926)
                     /gene="corA"
                     /locus_tag="PLR13_03975"
     CDS             complement(8865..9926)
                     /gene="corA"
                     /locus_tag="PLR13_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQC18522.1"
                     /translation="MTSKYHKRSRKTGLAPGSLVHIGSPYAEKSKITLMQYNEGFFEA
                     KDLESLKDFQADKDKEIITWLNVDGLTDIQLFEEIGRIFGLHPLVLEDILNTAQRPKM
                     EDYGGYIYLVARNFHDQENGSLLFEQISIILGKNFILSFREKESNLFKSIREKISQNK
                     GRIRKENTDYLAHALLDNIVDNYFVILENLEEKIEALEDDLIRQPDSSSLQAIHMLKR
                     KLILLGKSLWPLREAISALERSDSALINKTTVVYFKDIYDHTIAIIDTVETFKDMLSG
                     ILDVYLDTVSNRLNEVMKILTVIATIFMPLTFIAGIYGMNFKYMPELDMRWGYFGILG
                     VMLLVALFMVRYFRKKNWF"
     gene            complement(9938..11398)
                     /locus_tag="PLR13_03980"
     CDS             complement(9938..11398)
                     /locus_tag="PLR13_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HQC18523.1"
                     /translation="MMLLESFLSAVKSIRVQDVLDVMIIAIMIFALLTWFKTRASRFV
                     LIGIILLGGVYIAARFFQLYLTTIVLQSFFAILLFVLVVIFQEDLRRFFERLAMLGSL
                     GKKFPEISGLKSTAEIIAQTAANLAKKKTGALIVLRGNDPLERHINGGTKLDGVVSGA
                     LLESIFDPHSVGHDGAVIIEDNRISQFGCHLPLSANTSKYSNLGLRHTAALGLSERSD
                     AICVVVSEERGTISLAYMENLTVVPSAAVLHETLEQFYIRNTPTKKSHPALNWLKENT
                     LEKVIAIAMACILWMAFGYQRDIVRRDFIVPIEYKNIPRNWQIDEMQVTDARVVLKGP
                     EQAFRLLDERSLKLSLDLSGIADKKREFNLSADMINVPSNLTIADIVPAKIHVTASKR
                     IPWTFPVHVNTQNALAHHLSLQRLSVAPSKVRVLISAQTDPDQIKIETEPVDLKRILS
                     TTTVDTKIVLPIGVALPEGQSPAARVQIKVKQKASS"
     assembly_gap    11640..11739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11898..13394
                     /gene="trpE"
                     /locus_tag="PLR13_03985"
     CDS             11898..13394
                     /gene="trpE"
                     /locus_tag="PLR13_03985"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQC18524.1"
                     /translation="MYQPNFAEFEKLAQKGNLIPVYREILADVETPVSVLMKLRKKDH
                     VFLLESVEGGEKWGRYSFLGTDAGVVFRVQGDRVIIDEKGNVTTRHHHGGPLNFLREL
                     LNRYKPVALPGLPRFYGGAVGYLGYEMVRYFEELPPSPPDDLNLDDAVFIISDSLMIF
                     DNVRHTIKVVACTFTEDEGSLEAGYAASCRKIEEMIEIISASASHKIAAATEGDVNFQ
                     SNMTCHQYKDAIEKAKEYIAAGDIIQVVLSQRFETEYLNSSIDLYRALRFINPSPYLF
                     FLKMDDLTLIGSSPEVMVRKEDDTMELRPIAGTRPRGRTEQEDRALSDELLSDEKERA
                     EHVMLVDLGRNDLGRVAQTGSVQVNQFMMVEKYSHVMHLVSNVRAQLTKGKDAFDVLS
                     ATFPAGTLTGAPKVRAMQIISELEKVKRGPYGGAVGYFSFSGNMDFCITIRTMILKNG
                     RIYVQAGAGIVYDSIPESEHQESLNKARGMLQAIKLAAGGFEISTNQA"
     gene            13484..14053
                     /locus_tag="PLR13_03990"
     CDS             13484..14053
                     /locus_tag="PLR13_03990"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQC18525.1"
                     /translation="MILMIDNYDSFTFNIVQYLEQMGEKVNTYRNDKIKTDDIRRLQP
                     RAIFLSPGPCSPAEAGVTVEVIRKFYQDVPIMGICLGHQSIGYAFGATVSRAGRIMHG
                     KISHVKHDGQTIFAGLPNPFPAGRYHSLIVYPETLPDCLEISALTDEGEIMGLRHKKY
                     PVEGIQFHPESVLTPQGKRIIRNFLKYTK"
     gene            14069..15079
                     /gene="trpD"
                     /locus_tag="PLR13_03995"
     CDS             14069..15079
                     /gene="trpD"
                     /locus_tag="PLR13_03995"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQC18526.1"
                     /translation="MIKEAIEKATMKTDLPEAEMMAAMDEIMEGVATPAQIASLITAL
                     RMKGETVEEVSGAARIMRQKATRINACSSTIIDTCGTGGDKLNTFNISTTAAFVAAAA
                     GLTVAKHGNRAVSSGCGSADVLEALGVNISADQEIVEECVQQIGIGFLFAPKLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLTEMFAHVLKNMGTKKAFVV
                     HGLDGLDEATITGETRVAELRDGIVRTYNINPVDYFGRLDSLESIRGGDPSANAQITR
                     DVLSGKNGPCRHVVLLNAALAIVAGEKAKDIKEGLAIATDCIDGGAAVKKLQGLIEMS
                     NS"
     gene            15293..16063
                     /gene="trpC"
                     /locus_tag="PLR13_04000"
     CDS             15293..16063
                     /gene="trpC"
                     /locus_tag="PLR13_04000"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQC18527.1"
                     /translation="MLDKIIETKKEEVAQLKKETTEAQLKKEIAGFAPCRDFKKALTS
                     SDCNIIAEVKCASPSRGRLVTDFDPLRIARIYEQNGAAAISMLTDEKYFSGHKNYLKQ
                     IRPTVQLPLLRKDFIVDPLQIFETRIIGADAVLLIVRVLGEKLADFISLSGEVGLSQL
                     VEVHSQEELDLALAAGAEIIGINNRDLDTFITDIETSVKLKKRIPENNLVVAESGIRK
                     RKDIELLIKAGIKTFLIGEHLVTAPDIGKKLRELKGES"
     gene            16065..16727
                     /locus_tag="PLR13_04005"
     CDS             16065..16727
                     /locus_tag="PLR13_04005"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQC18528.1"
                     /translation="MTQVKICGIASKGDAFAAVNCGADALGFIFYPPSPRYIKPDDAE
                     KIIRVLPDKVVKVGVFVNEQPKNIKKLVETCGLDMIQLHGDESPDDCRLFPSQVLIKA
                     VELKNEFDLTPACAYPVAAILVDNRHAGRYGGTGKKSNWDLAAGLRGKIPLILSGGLN
                     AENVADALKTVTPDALDINSGVEASPGKKDHQKLARIFDTIRAFDTKHENFQSIFKKR
                     DT"
     gene            16724..>17830
                     /gene="trpB"
                     /locus_tag="PLR13_04010"
     CDS             16724..>17830
                     /gene="trpB"
                     /locus_tag="PLR13_04010"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQC18529.1"
                     /translation="MKNTLPDKNGHFGIFGGRYAAETLMPALIELEKAYKIAQKDRGF
                     EKEFEWYQREYAGRVTPLYCARRMSESLRGAKIYLKREDLNHTGSHKINNTLGQALLA
                     RRMGKKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKTLGAEV
                     VPVKSGTATLKDAMNEAMRYWVSSVRDTFYVIGTTAGPHPYPGMVRDFQSVIGKETKR
                     QIMKFEGRYPDVMIACVGGGSNAMGTFYPFRNEKNIAMYGVEAAGHGMNTSEHSASIN
                     GGKAGVLHGNKTYLLQDAHGQVRDAYSIAAGLDYPGVGPEHAYFHATKKVKYVTITDK
                     EAIKAFSFLSLKEGIIPALESAHAIAYAMKLAPKM"
     assembly_gap    17831..17930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18072..18866
                     /gene="trpA"
                     /locus_tag="PLR13_04015"
     CDS             18072..18866
                     /gene="trpA"
                     /locus_tag="PLR13_04015"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQC18530.1"
                     /translation="MKPIENKFSELRARNEKALIVYLTAGDPSLDITKQLIFALEKSG
                     VDILEIGVPFSDPTADGPVIQAAAQRSLKAGTTLKGVLKMVADVRKTSDIPIVLFGYF
                     NPIFAYGVKNFAQAARHAGVNGVLVVDLPYEEAKELRDYTDAVGMDFISLIAPTTDKE
                     RLKKIASKASGFLYYISITGITGTAAPKIGNIKTEVSKIRKITKLPIAVGFGISRPEQ
                     AREIGRFADGVVIGSAVIRLIDENKNNGDLSKILSDYISNIKKELR"
     gene            19042..20829
                     /locus_tag="PLR13_04020"
     CDS             19042..20829
                     /locus_tag="PLR13_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQC18531.1"
                     /translation="MKLTLVWFLQATAVIGYVNYTYDGYFMRLFMHWIIVCLLLIVLM
                     IPFDLKSFKIVPSVVRRITLVLILSFVHYFLLCFYVMTILGYKSWSGPFTFELLSAYS
                     GHIDVLLNIYGLSVEAAIIIFLFIWLLIFAGYYYYSGVAAEEFTANENDATQPHSRWP
                     VLVLFLVMIFAIVYSNSYKSWQMSEPLHIAWLNGHGCLRQAPQGIFNKRPLSGEKQST
                     TSAEPYEGITPRPLVLITVDALRSDQMGVYGAPRETTPFLSHLWRGGKLRRIDNAYSI
                     CTTSFCGMIGILSSKYWHQLNEPPVNLADVLKKYNYRNSFLLSGDTTNFFGLRQVLGS
                     HIDLFCDGSIEPKKYANDDRSVLGWLQTYNWKSADRTFLYIHLMSAHIIGLRDPRFKK
                     WLPDKEWDIPKLFYSQRAYQNNYHNGVLQADNTIRQIFEILDREGMLQRALIVITADH
                     GEFLGEFDNFGHGFEPFEPMVRIPMLVYDGLQSNYPERSLSSQVDVAPTMLYAIGAAI
                     PKDWSGIPFQLAAPRKAVYTACYDVSGVVADIGGQRFKFLQKRKGGKEMLFDLDTQKA
                     ESINLAAKPDMRKILASMRELNQLTQKSK"
     gene            20856..21344
                     /locus_tag="PLR13_04025"
     CDS             20856..21344
                     /locus_tag="PLR13_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18532.1"
                     /translation="MARFIFLTAAMGTALSGGLLGYVLCPLFSWYFFKDLNFIKYHHY
                     IIRLVFAFWRQVVELLYNPDYREMFYIPWTDPPINAPDPKRVRVRALWQHSDKGCGLC
                     NNCCTRRACPLHDMKHNQCKSYGSFFWRYFNCGRYPENTKQIHYYECKKWERYNCLSE
                     NE"
     gene            complement(21409..22251)
                     /locus_tag="PLR13_04030"
     CDS             complement(21409..22251)
                     /locus_tag="PLR13_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18533.1"
                     /translation="MKVIPADASTVVLLRSCPDASVRGIEVLMVLRNRKSSFVPGYYV
                     FPGGGLENDDFADGMERFIVGMDRLAASKILSDMRYPEQALGAWVAGIRETFEEVGIL
                     LAQKKDGSPVTIRAENERQRFCDHRKALISGKMRFIEMLATEELLLPLDRLYYFSHWI
                     TPEPFPQRYDVRFFVTEIPSDQAVIHDGVELTDHVWVRPAEAIRQYEQGRLDMVLPQI
                     MTLENIKNCKTVAEVIARVRKRNVPAILTKIKRIDGKDVEVMPDGAVFENRPPAYSWP
                     DKSD"
     gene            complement(22331..23845)
                     /locus_tag="PLR13_04035"
     CDS             complement(22331..23845)
                     /locus_tag="PLR13_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQC18534.1"
                     /translation="MTTPIQQKIRDKKRTAKQIAEMVKSGDWINHGAVGGDSTACTEA
                     VAKRLGGGKSDLHDIEFWLAGNFYPHPELQEIDPMQQYHCVHEHFFFPWNRKAREDND
                     VTSWTPWGWAGGIWAHHYRFANGERKNRGIDWWFNAATAPDNHGYFNMSYGTNSSNIF
                     KQTAKKIVFEVRADYPWAKGCRFNTIHIDDIDYWVEVDCEKYKWPQMNEKGIVARPME
                     QEIASHIMTIMRDRDVVQLGIGSLPSACVAAMADAGFKYLGIHTEMLNYGLIKMIESG
                     QVTNAYKRLDQGKSVWTFAFPVDVKWYYETVHRNQALAVYDIDYTNNLDVLRRVDNMI
                     AINNTVAVNLLGQMTCSFYGKRPISGTGGFFQFIIFAAQSKGGRSVAAMTSCSKHNTP
                     RIVPFLPEGSVVDVPAQFAQYVCTEYGMVNLRGLNGYERARALISIAHPDDRAWLEKE
                     ARRHGLMPPQFPANFLPEEGGNRRYPSYSDRRSYKMPMGSSIWGYDWDPIQSGK"
     gene            complement(24110..24307)
                     /locus_tag="PLR13_04040"
     CDS             complement(24110..24307)
                     /locus_tag="PLR13_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18535.1"
                     /translation="MHYRICKTVTIVLFTMLAMFCLSCTEAKCKIDQTVCNYDCPSTI
                     GVKQACEQKCNLLYDICRSQK"
     gene            complement(24421..26241)
                     /locus_tag="PLR13_04045"
     CDS             complement(24421..26241)
                     /locus_tag="PLR13_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC18536.1"
                     /translation="MSEFKPYNDTTIPGIFRNQVEKSRDIIFLRIRDENKKWKEYTYG
                     DISGQVDALASYFLKKGIQPTDKIAIYANNRPEWAVADLATLAIGAADVTVYPTNSGP
                     EAEYILNDSRAKICMCAGKFQVDNLLKEKNNLHYLQEIIVFDEIDSRYPIVVKFSSVL
                     QIGKASLNLDEIDKRLRNIDVQETATLIYTSGTTGNPKGAMLTHSNLVSNVKQFVEHH
                     PFPPRGTCVSLLPLSHALERTVGYNSAMHEGCTISYTKGAETLLGDLKDVRPTLAVYV
                     PRVLEKIYEGVMSQLGNAPPSKQKLFHASMGVGKKALPYILYNKPVPFFLNLQYKLFE
                     KLIYSKLRKALGLDRMVTIGVGGAPLSNTINEFFQIIGVEIHLGYGLTETTPITHLNT
                     YKHLKPIKIGTCGPAFPRTECKIAEDGEILIRGPQVMKGYYNRPEDTAQVLTKDGWFY
                     TGDIGTIDEDGYLTITDRKKDILITAGGKNVSPQVIEGAFVMHPLIEQIAIMGDQKKY
                     LVALIVPCFTELIKWAKSKNINETDPTKLIAYPEVVQKYKETIDELNKPLGRVEQIKR
                     FSLINHAFSQESGELTPTLKIKRKIVLANYQDRIEEMYKE"
     gene            complement(26651..27088)
                     /locus_tag="PLR13_04050"
     CDS             complement(26651..27088)
                     /locus_tag="PLR13_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18537.1"
                     /translation="MPIKRIFFKTRLAFLMYGFACMMRLAVLISKDFRAHLATHDYSF
                     IMGSPEEDTYRYFQFIRGRVQSVRGRRPSDFALIWRDNRSGGKVMIDLAAGKRKALNR
                     AVVHGLLTLEGEGKYISLLIETMNRLNRLFRHERKTNNRVKAG"
     gene            complement(27161..27730)
                     /locus_tag="PLR13_04055"
     CDS             complement(27161..27730)
                     /locus_tag="PLR13_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQC18538.1"
                     /translation="MEQTFTITVNVQELFMEFIPKLAGQYIQMRGDQEELKGTELKIT
                     LDISGSVYSYIIQDGVGFSIREGAVDNPHVRIAFSPDTLSKMADMKNIDMLIAMQKQL
                     TRKKFDVLTALSGKGVFQIKNADDTVSEITAIFNGAEKPIAVLRLSMQDANMISGGKE
                     SPINLFMSGKMKIEGDIAFAMKLQPLFVA"
     gene            complement(27980..29413)
                     /locus_tag="PLR13_04060"
     CDS             complement(27980..29413)
                     /locus_tag="PLR13_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18539.1"
                     /translation="MMAIAVLGIAMVPVTGQAVSRVPLNASGIYSATADDGSKIFLYR
                     YAPYTTGTPKFNTSATPVLIFTGITMNMNQYLSCTPPDKKDVYSSVYVPPVDQAPEWA
                     LNEDKTDYEPYIKADKMRYYSLAHYLWLKGYDPWFVNYRGTGRGEVRSDGTNANSLTT
                     LDTWATQDAPAAIAKVKSVTGKRMFIGGHSTGGLVSYDYLQGAYMESGIASSAWAKKA
                     YYKLCYSLGYMPHVRASADLAKKRNEDVKGFIGIDPAGVPSLPNLLDTPLFWTIVGSR
                     LYLPLDYLSDNLIQVLPSKPLVGLLEGMFGLLNKAAAGDSAISDFFDYLNFWVVEDMD
                     PCLEDWTVRYAVGSAAIRGFGHYMDMGLNNTLREHYLNGKENYLSSKLVKGVSAPNPG
                     RDGYYYYSENMARMTVPMIVFSSSTGSLVSPQATYDFIISKKTPTAYDEWYVLDGTGH
                     FDLAMGKKMPTEMFPRLGAWLDTVDAL"
     gene            complement(29905..31989)
                     /locus_tag="PLR13_04065"
     CDS             complement(29905..31989)
                     /locus_tag="PLR13_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC18540.1"
                     /translation="MTNDIITLNEFDGTALLKKGGIPVMDSILARDDKEIDVAANQLG
                     FPVALKICSEKILHKSDRGGVALNIADTSSLADAFRDMNNRFSDVPHAFLVQKMARPG
                     TELILGARRDPVFGPVILAGIGGIFTEIFRDTAIDLAPVDNTIACAMLGRLRGAALLD
                     GYRSQKALDVVAIAEALSSLSRLISDRPDILEIDVNPFIAYPDGAVAVDALVRLGGSH
                     ARSARKHPEPATVVPFFNPASIAIIGASRSPGKGGNIILRNLQKAGFGGAIYPINPTM
                     KEILGLPSYAKVCDVPAPVDLAMIVIPKTAVPEALEDCKAKRVTNIILSTGGYSDAGK
                     AGAEEQKIIIEQARKADIRIMGPNSIGTLNPSAGMATSIVGLEPIKAGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDINESDLLEYLAEDWVTLTVGMYLEGVIDGR
                     RFIRAAEKASRKKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRTGLVRTHN
                     PEAFFDTLSAFEKLPLPRGNRMGVFSITGMGCVAATDAAEEYGIVLPELNPATLKKLA
                     EVIPAWAPVRNPIDTWSAIEQHGSKKTSIHIAQCMLDQRDMDALLIIFVLMPESMFDI
                     PEAFAELFRNHPDKPVFASYYGGTAREIAHVHEGFSRLGVPCYPTPERAMFAFGRMVE
                     YARHRGRIRTRP"
     gene            32199..32939
                     /locus_tag="PLR13_04070"
     CDS             32199..32939
                     /locus_tag="PLR13_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18541.1"
                     /translation="MKITFLGTNGWYDTDCGNTICTLIETKTAYILLDAGNGIYKADK
                     YIKKNLPVYLLLSHFHIDHMEGLHILAKFRFTSLEIIGQTGTKKTIGEFLSPKYSLPV
                     TRLPYPCNVTDINEGWHRRPFRFRALPLEHASRCFGYRLEMEGKVISYCTDTGYCKNS
                     EELSVNADLLISECAFLSGQQSKTWPHMNPQLAARLAVKSQAKRLALTHFDAALYTTR
                     AKRAKAQMQAKRIFPHTFAASDGLSIVI"
     gene            complement(33375..33911)
                     /locus_tag="PLR13_04075"
     CDS             complement(33375..33911)
                     /locus_tag="PLR13_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18542.1"
                     /translation="MKVFIIVGMPAAGKNIARIYAGSRDIVYYSTGDIVRAEVKARGL
                     EVDAATTSEVSDDLRGEDGLGVTRLALEEILKTGADVGFLEGMRSWQEIELIREKTDG
                     VVVAVLTPRGLRLERVCSRGRDDDSPLGFNERDQREIAYGTAIPIALADAYILNTGTM
                     DDAMEQLEAIVQREMTPS"
     gene            complement(33938..35134)
                     /locus_tag="PLR13_04080"
     CDS             complement(33938..35134)
                     /locus_tag="PLR13_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQC18543.1"
                     /translation="MNHVLRRKIFILSIMAVVAGLLIYGFLPKTRDVDIVFVTRGPLQ
                     VTIEEEGRTRLKDRFTISAPTAGYLQRIHAKVGDNVQQGQIVAVLEPLQSQALDPRSH
                     AAARAAVSAAEAALKAAVEREKVAAADAAYLDQRRERLKALYDKGSIARDQFDQIHAE
                     AQKARALQLSSAAEVHRAGSELEQARVALRNFSAVRSQGNAVAVSSPVNGAVFRIYHE
                     SEGAVNIGAPLMDLGDPKNLEVRVEVLSSDAVKIRQGTHVLFKRWGNDEPLTGSVRLV
                     EPAGFTKISSLGVEEQRVLVIVDITSPPEQWSVLGDGFRMEAHFTIWEDDNVLQIPAS
                     ALFRAGKDWAVFVEHDGKARKRVIEIGQRNGLTAQIISGLKENEKVIAYPDDTISEGT
                     KIRQRK"
     gene            complement(35170..37536)
                     /locus_tag="PLR13_04085"
     CDS             complement(35170..37536)
                     /locus_tag="PLR13_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQC18544.1"
                     /translation="MMKTLNRKLWRELWLMKGQVFAITLVVMSGVATFIMFISTMHSL
                     DFTRSKFYREYNFADVFVNLKRAPETLKYKINDIQGVHQVETRVCAQAKLDIRGFKES
                     VTARITSIPDDGDPLLNRLYIRKGRLPDPARDNEVAMNETFAQAHGLNPGDKFAAVIN
                     GKWKRLTVTGIVLSPEFILLMKTGAMTPDFKRYGALWMTRKAISKAYDMEGAFNDVVL
                     TVYPGTALSDVVRDLDNILGRYGGFGAYGRKDQISHRLLSEEFRQLKTSSRIYPTIFI
                     CVAAFLLNIVMSRTISTQREQIGTLKAFGYNNLSIAVHYATMVMMIIATGVLLGIAGG
                     IWFGKMLGALYMETYRFPFFHYILHPQVMIAVVVISVAAALSGTMHSLWNAARQTPAE
                     AMRPEAPVKYRVSLIEKTGIGRWLSQPSRIIFRNIERKPVKTVLAIIGIATACATMIC
                     SGFFRDCIDYMVHIQFVLSQKEDMTVSFVEPTSYRAVYELKRMEGISSGEAFRSVPVR
                     MKFGHRSYKTVINGIQPDSHLNILLDNHLKSISMPPSGILLTDYLGEILGVKAGDLLM
                     VEALEGSRAVRQVPVVAMAKQYLGVMGYMNLEALNRLINEGDAISGAYLVTDETHREK
                     LFQLFVQMPRVAEIVVRQNEIRSIYEIMAKAMLFFMFIATVMACSIAFGVVYNSARIS
                     LAERSRELSSLRVLGYTRGEIAYILLGELGFITLVAIPLGFIIGYWICAYVAHALTTD
                     LYRIPMMIELKTYAQAALVVIVSAVISGCIVRHKLNNLDLVEVLKTRE"
     gene            complement(37538..38254)
                     /locus_tag="PLR13_04090"
     CDS             complement(37538..38254)
                     /locus_tag="PLR13_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18545.1"
                     /translation="MNQQKEKFFQVQDLTKVYQMGEVAVHALRGVTMELYAGELVVLL
                     GPSGSGKSTLLNIMGGLDTATGGVVSYRGKELTKATEKELTEYRRFHVGFVFQFYNLI
                     SSLTARENVSVVTEIAPDPMAPQQALDLVGLSDRLNHFPAQLSGGEQQRVAIARAISK
                     KPAVLLCDEPTGALDSQTGIVVLQALERINRELGTATAIITHNVDIAGMADRVIHLSN
                     GHIASVEINKVKKSASELQW"
     gene            complement(38312..39049)
                     /locus_tag="PLR13_04095"
     CDS             complement(38312..39049)
                     /locus_tag="PLR13_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18546.1"
                     /translation="MKKIRCAPYWMVFVLTALVFFTTGPAASAAGLFSAFETVSRQDG
                     GLNTAIGYRYHQDTYEKGNGLKIRQNEIYSQAAYGADNAWEIYARLGIADLKIGDVFR
                     ESEEVFATLGGKVFYPVSGKLGVGAFVQGTYYLNDFSDTVERRKHVQLKDLWDVHFGV
                     ALQATLPCGSTMYAGPYLYYSEGTLSLSSNVSDWGYAAGRYSVQNTSIAGGFAGLDIP
                     LAKGFRLNVEGQYADRFSAGAAISYTY"
     gene            complement(39050..39979)
                     /locus_tag="PLR13_04100"
     CDS             complement(39050..39979)
                     /locus_tag="PLR13_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HQC18547.1"
                     /translation="MKSRLVFLVSFIWMLSGGAPGSAHAFVDLPPEQTSPPPVVLTVS
                     PDRPANQFAAAAVVDPMEEEYTGDEFDDYAGDNAKEEKVSIADPLEPFNRAMHEFNDK
                     MYFWAVKPVAQGYKAVVPEPARISVDNFFSNLGFPARFLSCLLQADFKGAATEAGRFT
                     INTVWGIGGLLDPSSGKELNLQEQNTDLGQTLGVWGVGQGFYIVWPVFGPSTPRDTVS
                     IAGNYFLDPVAYLNPWSARLGVNVLDKVNAASLRIGDYEALKNAAIDPYVAFRDAYIQ
                     YRYKQIKARKAKIFSGSSAAEPFPETVRPDKVD"
     gene            complement(40047..40643)
                     /locus_tag="PLR13_04105"
     CDS             complement(40047..40643)
                     /locus_tag="PLR13_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQC18548.1"
                     /translation="MKKLFTQTAIILVIVMFSLPVYAGAALDSVQKCVNEVIGIVGDP
                     KLKSESAKELKKEKLRVLYKQMFDEIEFSKRTLTRNWNNFTPAQRTEFVNLFEQVLET
                     AYGDKVLSYTNEKVVFYKETTLSATQAEVQSKIVTSSKEIPVFYRVILKDGKWKVYDV
                     VVENVSLVQNYRTQFNDILSKDKPEKLLEVLRNKVKQH"
     gene            complement(40678..41133)
                     /gene="mlaD"
                     /locus_tag="PLR13_04110"
     CDS             complement(40678..41133)
                     /gene="mlaD"
                     /locus_tag="PLR13_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HQC18549.1"
                     /translation="MKKYEIETVVGIFVFLGLLCIGYMTVKLGNVSFLEAASYPLEAK
                     FTSVNGLRIDSSVKVMGLTVGKVKQIAMDQDNQRAVVKLNIQKGIKIYDDAIASIKTE
                     GLMGDKYVSIDPGGGGSLLEPGGIILETQSPLDIETLISKYAFGDVKKQ"
     gene            complement(41138..42382)
                     /locus_tag="PLR13_04115"
     CDS             complement(41138..42382)
                     /locus_tag="PLR13_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC18550.1"
                     /translation="MNIPLIEFKNITKRFGEQTVLDQVNLQIYEGEVTTIIGLSGSGK
                     SVLLKHIIGLLKPDEGTILFRGEPINDMKKTELAEAMSKISYMFQSNALFGSMTVYEN
                     IALPLLENTAMKKNEIHDRVMARIEQTELTEAAQKYPSELSGGMQKRAALARALITDP
                     QVVLFDEPTTGQDPVRKNAILSMIAQYQKKFNFTAVMVSHEIPDVYFISNRILALYDK
                     SIVFQGTPEELSNFDHPFNDEVIRSLEGLQKELTGLYSRRQFKVLHRSQLQSRKSDEG
                     YCVVVFDLSDMNSIVSAIGYDRAQETIHSMGKYIDKHFDAIGGFSTRRKINEFVTVLP
                     FSDIAETESIVKDFMEDFQKSGMSEIWSEAQKNDPQTRCVDFSVKAGMAEGTPVAEID
                     SIVKLAKAHRKEIGRLQCAVKE"
     gene            complement(42390..43175)
                     /locus_tag="PLR13_04120"
     CDS             complement(42390..43175)
                     /locus_tag="PLR13_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18551.1"
                     /translation="MSKPFALIGRNVIRWINSIGAATIFLVLALLKTFHPKQFQKVIQ
                     QIYYIGARSTTIIMLVSLFTGMVLGLQSYHALVQFGAEGSLGTLVSLSLIRELGPVLT
                     AIMITARAGSAITAEIGVQRISEQIDALETMRIDPFRYLVTPRITASIVSFPLLTTVF
                     DLIGIIGGYVSAVALMGLNAGVYSHSIRSYVDMEDLTNGFVKSLVFAVIVATVCCYQG
                     YFVHMREDSKGAKAVGLATTSAVVISCVLILVADYVVTSLLMV"
     gene            complement(43317..44195)
                     /locus_tag="PLR13_04125"
     CDS             complement(43317..44195)
                     /locus_tag="PLR13_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC18552.1"
                     /translation="MTQTERILDIPSLNHHDIGGAHLPYLYYEGETPQILFAHATGFL
                     PWLWHPILEELTPPRRQAWAPYICNYRDCDPESGGLSWDVIARDLISFCSVQNISDNL
                     AVGHSMGATVVTIASALFGLQPRGMILIEPIFLPEDFYTIDIQLKDHPLASLSIRRKN
                     GWKDENAALEYLKSKSLFAGWDEQMLQFYLQYGMEKQAGGDLKLTCAPRNEAALFMGG
                     KSTNPWPLLEKLTCPVLILEGEKSSNREFIDLPKAASLLRDGRYQLIPDAGHLIPMEK
                     PKEIAKIIKEFLADVF"
     gene            complement(44282..44830)
                     /locus_tag="PLR13_04130"
     CDS             complement(44282..44830)
                     /locus_tag="PLR13_04130"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HQC18553.1"
                     /translation="MDSFVPKKIFFTKGVGTHKDELHSFERALRDAGIEKCNLVQVSS
                     IFPPRCKMISKTQGLKQLTPGAITYCVMSRCCTNEPKRLMAASVGCAIPADKSSYGYI
                     SEHHAFGQNEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTLNV
                     TQSTICKDNKYTTVIAAAVFVF"
     gene            44992..46380
                     /locus_tag="PLR13_04135"
     CDS             44992..46380
                     /locus_tag="PLR13_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HQC18554.1"
                     /translation="MKKDYTIRQNKLKGSGMKVKYVCVVLALFLVSCYLQPPDTLQDV
                     KDVTDLPQDHTARLADVPADKVLLTRDSQTAMNEHYDKTFFSVWHHPEPVHALPERLS
                     AIFTQFAAHPGYGENKRKHSTAWLKKLQQNAWLENYPNTLQPAITVRSTNLRMLPTNG
                     PHFYKPGGDINGWPFDNLQISSVAANTPILICHISRDRSWALVETSFAFGWIPAEDFA
                     RVDDPFMQTWQTSRYATVTRDKIPIVDANGNFSLRTSIGQMFPLITEMADKLEILIAS
                     ADANHQAIIQRVFISREIMNIKPLPFTYANATRIANELIDEPYGWGGLYGNRDCSAMT
                     RDFFAPFGIWLPRHSADQAKKIGIYIDLEAMEPQEKEKVILEQGIPYLSLLWRRGHIM
                     LYIGERNGRALIFHNVWGLRITDARGEEGRKIIGKAVITTLHPGKELTNIDPDALLIK
                     NLSAMTILAPAK"
BASE COUNT        11999 a        11484 c        11075 g        11797 t
ORIGIN      
        1 cagttgagaa accacgtgta agttgtcaaa atttcaactt gacatccatt tattcgctgt
       61 gctacggaat ttccaacttt aagaaacacg tgagccgact atgttcaaaa ccgtttccaa
      121 aaacaactgg tggtggcaga tttcaaatgt ctgcccataa tctcgggaaa atatgagcat
      181 gggcagggaa gcttcttccc atgactctcc ggtaatagtt aaagccatgg gaatacaatc
      241 ccgtggcttt cttaatttca ggatcaaaat actttatttg aaggagaaag aatatggaac
      301 aggtaaaagt agtattgcag gaccgtgaga tacccaaaaa gtggtataac atcatggcgg
      361 atctgcctac gccgatgaaa ccgccactgc atcccggaac cggtcaaccg gtaacccccc
      421 aggatctggc ggcaattttc ccaatgaaca ttattgaaca tgaggcctcc aacaagcgct
      481 ggatcaaaat tccggaagaa gtcctgcaaa aatatctatt gtggcgtcct tctcctcttt
      541 accgggccta taatttcgaa aaactgctca attgccccgt caaaatttat tataaaaacg
      601 aaggcgtgag tcccgcgggt tcacacaagc cgaacgccgc cgtaccgatg gcttattata
      661 acaaggtcgc gggaaccaag agaatcacga cggaaaccgg cgccggccag tggggcagtg
      721 ccctggccat ggctacgcaa atgttcggtc tggagtgccg tgtatacatg gttaaaatca
      781 gctacgaaca gaaaccctac cgtcgcctga tgatgaacac atggggcgcg gaatgcattc
      841 ccagtcccag cacgctgacg caatcaggca gagattttct ggccaagtat cccgattctc
      901 ccggtagcct cggcatcgcc atcagcgaag ccatcgaaga tgcggtcacc agcaaaatca
      961 aaacccatta cgcgctgggc agcgtgttga atcacgtgtt gatttatcag tccatcatcg
     1021 gcctggaagc ggaaaaacag atgcagaaac tcggcatcta cccggacgtg gttatgggat
     1081 gctgtggcgg cggaagtaat tttgccggca tcgccgcccc gtttgtgcgt gataaaattc
     1141 acggtaaaga tgtgaaaata gtgggcgcgg aaccgtcctc ctgtccgacc atgaccaagg
     1201 gcccctttgc atatgacttc ggcgatctcg ctcagaaaac accgcttctg cccatgtaca
     1261 cactcggcca tgactatgtg ccggcgccga ttcacgcggg cggactgcgc tatcacggca
     1321 tggcgccgat catcagtcat ctggtcaagg aaaaacttat cgaacccaga gcctacgatc
     1381 agttgaagac atttgacgcc ggcgtcaaat gggcccgttc cgaaggtttc atcccggcac
     1441 cggaaaccaa tcacgtactg gcagcggttg ttgacgaggc gatgcgcgcc aaaaaggaag
     1501 gaaaggaaaa aaccattctc tttaactgga gcggccacgg tatcatcgat cttgccgcgt
     1561 acgatgctta ttttgcaggt aaattgaaag cttatgaact gcccggtcac gagatcgagc
     1621 gagcattaaa agccattaaa aatttcccca aaccctagta aaccctgagt aacaacagat
     1681 aaggccgttg agaaatttat atcttgacgg cctttctttt ttcagtaaag ctgaacccct
     1741 tgaaaacaca acacgacaac aaaatgatcc gctgcccgaa gttaggagac gaaatgactt
     1801 ttgcttactg tctgcgtgaa ggaggcaatc ttccctgtgt tcgaatcatc cgctgctggt
     1861 ctcctgtttt cgatatagaa tcttttttga aggggcatct atctgaaaaa cgttggctca
     1921 agtttatcaa taccaaagct ccggacaaaa taacaagctt gatagaactg attgaggcgg
     1981 caaaagcaaa aaaatgaaaa ctctggattt attcgatcag gggaattccg gcaacgtcaa
     2041 agacgcagcg ccgctggctg agcggatgag accacagaat atttacgaat acatcggcca
     2101 gaagcatcta ctgggtgaag gatctctgct caaacgggcg atcgaatccg acaagctgtt
     2161 ttcaatgatt ttctgggggc cgcccggctc aggcaaaacc acattggctc gaattctggc
     2221 caatgaaaca aaatcaaaat ttgtcagttt ctccgccgtc ctttccggcg tcaaggaaat
     2281 ccgcgcggtc attgacgaag ccagagaatt gctggagaga aaaagcatca agaccatctt
     2341 atttgtcgat gaaattcacc gtttcaataa agcgcaacag gatgcttttc tgccgcatgt
     2401 ggaaagcggc ttgatcacat taatcggcgc aaccacggaa aatccctctt ttgaagtcat
     2461 cgctcctcta ctctcgcgga cacgggtgct ggttttaaaa cctttttcag aagaaaatct
     2521 ccttgctatt ctggaggccg cccttaaaaa caagcaaaaa ggcctcggca atatcgatgt
     2581 gaaaattgat gccaatgttc ttcgctatct ggtcagtctg gctgacggcg atgcccgaag
     2641 tgcgttgaat aatcttgaag tgctggtatc catggtacaa aacctgccgg acgatcagag
     2701 ggcaatatcg ctgaaattcg ctcaggatgc cctgctgaaa aaatcgcttt tatacgacaa
     2761 agacggcgaa gaacattaca atttgatttc cgccctgcat aagagcttgc ggggcagcga
     2821 tccggatgca gctctttact ggctggggcg gatgctggcc gccggagaag atccccttta
     2881 tatcgcgcgg cggatgatcc gctttgcttc tgaagacatc ggtaatgccg atcctcaggc
     2941 gctggttgtc gccgttgccg cccagcaggc atttcacttc atcggcctgc cggaaggcga
     3001 actggccctg gctcaggcgg ctgtttacct ggccacagcg cccaaaagca atgctcttta
     3061 cgtcggttac ggcaaagtta aaaataccat caataccaaa gggtatttgc ccgtgccatt
     3121 acacatccgg aacgctccga ctaaattaat gaaagacctg aactacggca aagattacaa
     3181 atacgctcat gactacgaag agagttatgt tccgcaggat tatttgccgg aagaaatcaa
     3241 gggagagtta ttttatcagc ccaaaggcgt gggctttgaa aaaacaatta aagaacgact
     3301 caattactgg cgaaataaga aaaaagaagg ggaaaaagaa taaagcgcct agaatcgcac
     3361 taagtttcgc atgtgggata tccccatgtg ctccgcacaa gggacaattc aacttaccaa
     3421 tttcaatgcg ttctgctttt gaaattggag tttcattgat tcgacttatg tctcaaactg
     3481 cacttcgctt gttctaccac ggcactacgt gcctggtata gttcggctaa cgcttcgaac
     3541 atcatttcat tcgttctcaa cgagtcctca ctatcagcga gtgtcaaatc ccgttacgct
     3601 acgcttcggg gataattcca cttgcgcttc gaacttcgct aagctcgttc tcagcgagtg
     3661 tcattaaaaa gtaagggagg cttggtcgga gcctccctta aacatgaaga gtgaaggttt
     3721 ttataccttc caggttattt tgttgtcggc taatcatacc cgttattttt cagtcgacaa
     3781 tttacccgcc cgtatttaaa gcatttgccg taccagaatt ctaaatattt tacataatat
     3841 taatattatt gacaaaatat gtatattaat gccttcttca gcgaatatcc gcgacttgtt
     3901 tagcggcaaa aaatgtcgaa tcatttttgt taatttttaa aaatcataat aattacaatt
     3961 aattaagttt ttttgacgct gtttgtccgg cattcgacaa aattgtcgga taaaaaagga
     4021 ttaatcgttg acttttcttt caaatagctt aaaatgaccg gcaaaatcaa ttattaagga
     4081 taacaatgcg tcttttcaga aaaaaagaca tcgtcgttca tcgcaaagct cataattaca
     4141 atgacccctt ccttcaacct ctggccgtgg ctatcgtcag tgcaattttt gtcgcattaa
     4201 ttctgattac aggatttctc gagcttcgac gcagcgaaag caacctgata gcctttatgg
     4261 aaaatcaggc tttgagcact atcggcgttc ttcagcgctt aacggaagag aatttaaaaa
     4321 gcattatcgg cgtaccggat aagaacgctc cgcatgaaaa aacgtccggt cgtgacgaag
     4381 catcctattc taaacaatgg gttatccagg ctattaccga actggccaaa gatttggatg
     4441 aacaatggcg aaaaggggac atgaccgatg agtccctgcg tcaattcgca tcggacaata
     4501 atttttcgta tgtggggatg ttgaatgctc aaggaaacgc tgtctttcag agcggccctt
     4561 tgcagagaga cctccttgag gaaaacgagc ttacgcacaa aggtcgtaaa ttgaccacgc
     4621 tggaactgat atcgaaaata ggagagaaac aaggagtagg atttattgca ttaagccgta
     4681 aggacaatac cggagcggtg atcattagcc tggacaaatc cggatccgtt tactggattt
     4741 taaaaattgc cggtgaaagg ggaatggaca agatcggcga aggccacggc atcgtttata
     4801 tgcaaatctt taatgaccgg gacaagctgc tcagcagtgt cggccagccg gcccccgctt
     4861 taaatcacag aatcttcaat ctaaaggaaa ttttgtccgg aaaaataaaa attgtcagcc
     4921 ggaaagtgga ttatgccgac aataaaattc tcgacctggc ggcgccgttg tatctggata
     4981 aaaaaattgt gggtattgtg cgcatcggcc ttgaccgaaa ctccatggat aagagtcttg
     5041 tcgaaaatag acaacatatt tttgttttct cgatatttat cgtcctgatc gccttgttgt
     5101 ccatgtggct gctttaccat gaccagaacc gccacctggc cggcattgtg gaaatggaac
     5161 gccaattgga aaaagctgaa cggctttctt ctctgggtca actggctgcc ggagtagccc
     5221 atgaaatacg caatccattg aacgccatca gcatggccac ccagagattg aaaagagatt
     5281 tcgtcccctc cgaccctcaa aaggttgacg attttcagaa tttatcgggc atcatcaggg
     5341 atgagattaa acgcctcaat ggaatcattg aagaatttct taatttttcc aaaagccgcc
     5401 gcctggattt ctgtaaattt tccatcacag aactgctgca aaaaatcgtt agcctgatga
     5461 aagaagaagc cctgttgcgc ggaattaccc ttgaaacaac ctggaaacag gagtccgttc
     5521 caatctccat ggacgtgaac aaacttcagc aggcatttat caatctgatt aaaaacgcca
     5581 tggaatccat aacggcggaa aaaggcagaa ttgtgatttc ggtggacaag gaaggcaaaa
     5641 attacatcgt tgtaaaaatt tccgattccg gttgcggcat gaccgaagaa gaactcgaaa
     5701 aaatctttaa tcccgaatac accaccaaag aaaaaggggt ggggctggga attccactgg
     5761 cctttgaaat catccgtgga cacggcggtg atatccgggt aaccagccgg aagaataaag
     5821 gcacaacatt tgaagtgatt cttccccggg aaagattgaa tgaagtttca taatcaaaca
     5881 aggatgacca tttcatgcaa aagctgagca ttctgattgt tgacgacgaa aaatcccagc
     5941 gggaactgtt acgcgatttt ctccgaagcg aaggccatac ggtcacagaa gcggataacg
     6001 gagaaaaagc aatcaaaatg gttttgggcg gccatttcga tctgattctt ctggattaca
     6061 aaatgccggg gatgaacggt ctggaagtgc tcaaagaagt caaacgaatc aatccggaaa
     6121 tcgatgtggt gatcatcacg gcatacggca ctgtcgagac ggcagtggat gccctgaagg
     6181 ccggcgccat tgactatatc accaaaccga tcgtggaact ggatgaactg attattctgg
     6241 tcaacagaat agcggagcga cgacaactca tccaggagaa caggctgctg aaggatgact
     6301 tgagcaaaca cggcattact gcagataaaa ttatttatca gagccggcaa atggcggaac
     6361 ttatcaatct ggccggccgc gtagcgccca gcaaagcttc cgttttgatt cagggcgaaa
     6421 gcggcacggg taaggaattg ttggcgcggc tgattcatca attgagtccc cgtgccgata
     6481 aaccgattat tattgtgaat tgcgttgcac tccatgaaaa tctgctggaa agcgagctgt
     6541 tcggtcatga aaaaggcgct tttaccggag cgtcctcgcg ccgcatcggc cggtttgaag
     6601 aggccgacgg aggcacgatc tttttagatg aaatagggga gctgagcccg accatccagg
     6661 tcaagctttt gcggtttctt caagaacgtg aatttcagcg gctgggcagc aatgtcaatc
     6721 tccaggttga cgtgcggttt atcagcgcca ccaatcgcga cctggaaata caagtggcag
     6781 agggaacttt ccgggatgat ttattttacc gtttaaaagt tgttacgatg tcgctgccgc
     6841 cgctcagaga gcgcaaggaa gatttacctg tcttgattga ttattttatc gataagtttg
     6901 ctaaagaaaa cggcaaaaac ataaaaggat ttacggctga agccagggat ttacttttaa
     6961 aatatgatta cccgggcaat gttcgggaat tagaaaacat tctggagagg gctgtcgtca
     7021 tcgcacgcga acaatacatt accaccaatg atctgccgtt caaatctgat ttcctgcttg
     7081 aagattcaga aaagaaacct ttcggcgcgt taaaagactc tctggatgaa ttggaaaaaa
     7141 aattaattat tgaggctatg gaaaaaaccc agaacaatca gacccgggcc gccgatctgt
     7201 tgggaatatc ggaacggatg ctgcgataca aactcaagaa atatggtctg aaaggatgaa
     7261 atgaagcatg tcaaatacga acgaccagaa tctgtcgcaa ataatcataa aattgaaacc
     7321 aaaattaata ttcctttata tttttgtgcc aatttttttt ttagtcatgg cacatattct
     7381 gggtcaactt gctattttta acatcattcc atgttttcgc ggttatgatt tattctcagt
     7441 gttttttaac cttgatggag aggcaaatat tcccacgctt tacactattt tggaatggtt
     7501 cctttgtgtc atttttctgg tcttaattgc tttttttgag agaaacacac agtcctcccg
     7561 ttatagatac tggttgggat tggcgataat tttcagtttt ctattgatcg acgaattcat
     7621 ttcctttcac gaaatttcag atcatatatt agctctgttt aatataaaga tcgtgctttt
     7681 ccctactaat gcatgggttc ttatttactt agttgttctt gcaccgattt ctctcgttta
     7741 tttacgtttc atgattcatt tgcctaaaaa aacaaaatat ctgtttgttt tttcctttat
     7801 catttttatg ctgggtgccg caggaataga agcgctggaa ttgcgccatg tggagatata
     7861 cggacaggat aaatattatt atctatattt tgttatagta gaggaaacat tggaaatgat
     7921 gggtttgttg atatttattt atgctctggc atcttacctg gcaaatgagc tagggatatc
     7981 gcagattaaa atactgccag agaagatttc gcacgaatcg actgataaat cggcatagcc
     8041 gctatcctca tgcctgccct gctatcaagc ccttgtaacc ccttgcgtta aagatttatt
     8101 ctcatacgcg cgtattgttc ttgaaatttt tcaaaaggat taggggaagg gcaaagccct
     8161 ctgattattt ttgctgatat cttgttttta agcgaaacaa aaaacgccat aagaaagctt
     8221 tttcccctga agaaaagctg tgaaaattca ttggctaacc ataaaaaagc cgccttttag
     8281 ggcggctttt ttatggttag ttgagttgaa tgactaacaa gctaaacgtt ttttcatggc
     8341 tgtcagggcc tgaaaatgct ttctctcctc ttcgataatc ttctccagcg catcgtgctg
     8401 tgccgcaggg accatttttt tgatttcgtg ataataaaga atggagtcca gttccctctg
     8461 aatggcaaat tccagggcag atgtggtatc cgtaactttg gccaactgtt tatccattat
     8521 ttccttggtg aagatcaaat tattatcaac gtacgcacgg agatattcgc cgaattcccc
     8581 ctcataactt tcctgaaggt tggatttttc cataccggca aaaagttttt caaatgtttt
     8641 cttatgagca acttccgcct gcgccagccc ggcaaaaagc ttcttttcgt cttctttctt
     8701 ggcaatctgt tcggcaaatt tataaaaatt aacgccgttt tcttcaattc tgatagcgac
     8761 ttccacgata tcgcttgctg caaaaacact catcttcatt gcctcctgat tcttaaataa
     8821 attctgggta aattttgcat attttatgat tgaatgcaac aaaattaaaa ccagttcttt
     8881 tttctaaaat atctcaccat gaacagtgcg acaagcagca tgacgcctaa aattccaaaa
     8941 tacccccagc gcatgtccag ttcgggcatg tacttgaaat tcataccgta tattccggcg
     9001 atgaatgtca ggggcatgaa aattgtcgca atgaccgtca gaattttcat aacttcgttc
     9061 aaccggttgc tgacggtatc cagataaaca tccaggatac cggaaagcat atccttgaat
     9121 gtctccacgg tatcgatgat cgcgatggtg tgatcataga tatccttgaa gtagacaacc
     9181 gttgttttgt taatcagcgc cgaatccgac ctctccagtg cgctgatagc ttcccgcagg
     9241 ggccacagcg atttgcccag cagaatcagt tttcgtttga gcatgtgaat tgcctggagg
     9301 gatgagctat cgggctgtct gatcagatcg tcttcgagag cttcaatttt ttcctccaga
     9361 ttttccagaa taacaaaata attatcgaca atattatcca gcagggcgtg ggcaagataa
     9421 tctgtgtttt cctttcgtat tctgcccttg ttctgactga ttttttcccg gattgatttg
     9481 aaaagattgc tttctttttc ccgaaaagag agaatgaaat ttttccccag aatgatactg
     9541 atctgctcga aaagcaaact gccgttttcc tgatcatgaa aatttctggc cacgaggtaa
     9601 atatagcctc cgtaatcttc cattttcgga cgctgagccg tatttaaaat atcttcgaga
     9661 acaagcgggt gcaacccgaa aattctgcca atttcttcga ataattgtat gtccgttaat
     9721 ccgtcaacat tcagccaggt gatgatctct ttatctttat ccgcctggaa atccttcagg
     9781 ctttccaggt cctttgcttc aaaaaaacct tcattgtatt gcatgagagt gatttttgat
     9841 ttttcggcat acggactgcc gatgtgcacc agacttccgg gcgcaagacc cgtttttctg
     9901 gatcttttgt gatacttgga cgtcatgtct gcctcattta agaagaagcc ttttgtttca
     9961 ccttaatctg cacccgggca gccggcgatt gtccctccgg taaagcaaca ccgatgggaa
    10021 gcacgatttt tgtatcaacc gttgtcgtgg ataaaattct ctttagatca accggttccg
    10081 tttctatttt gatttgatcc ggatccgtct gtgcactgat caaaacccga actttggacg
    10141 gcgctacact taaccgctgc agcgaaagat gatgggccag agcattttga gtgttgacat
    10201 gaacagggaa tgtccaggga atacgtttgg aggccgttac gtgaattttg gcgggaacaa
    10261 tatccgcaat ggtcagattg gatggaacat taatcatgtc ggcgctcagg ttgaattcgc
    10321 gttttttgtc ggcgataccc gatagatcca aggataattt caaagagcgt tcgtcaagca
    10381 gacgaaacgc ctgctccgga cctttcagca cgacccgggc atcggtgacc tgcatttcat
    10441 cgatctgcca gttgcgcgga atatttttgt actcaatggg aacaataaaa tcccgccgta
    10501 caatgtccct ctgatagccg aacgccatcc acaaaataca ggccatcgct attgcaataa
    10561 ctttttccag tgtgttttct ttaagccagt tcaacgcggg atgcgatttt ttcgtcggcg
    10621 tgttgcggat ataaaactgt tccagcgttt catgcaggac ggccgcgctg ggcaccaccg
    10681 taagattttc catgtaggcc agagaaatgg tccctcgttc ttcggatacc acaacgcata
    10741 tggcgtcgga tcgttcggaa agaccgagtg ccgcggtatg acgcaatccc aaattggaat
    10801 attttgaggt attggcggaa agcggcagat gacagccgaa ttgactgatc ctgttgtctt
    10861 ctataatgac ggccccgtca tggccgacgg aatgcggatc aaaaatgctt tccagcagag
    10921 ccccgctgac cacgccatcc agtttggttc ctccgttgat gtgcctttcc agggggtcat
    10981 tgccccgcag aacaatcaaa gctcctgttt ttttcttggc taggtttgcc gcagtctggg
    11041 caatgatttc cgccgtgctt ttcaagccgc tgatctccgg aaatttcttg ccgaggcttc
    11101 ccagcatcgc caacctttca aaaaagcgac ggagatcttc ctgaaaaatg accaccagaa
    11161 caaaaagcag aatagcaaaa aagctctgca gaacaatagt ggtgaggtaa agctgaaaaa
    11221 agcgcgccgc gatataaacg ccgcctaaca gtatgatgcc gatgagtacg aaacgggacg
    11281 cccgtgtttt aaaccaggtc aaaagagcaa agatcatgat ggcgatgatc atcacatcca
    11341 gaacatcctg cacccggatg ctcttcacgg cggataaaaa ggattccagt aacatcataa
    11401 atttcttccc gatacgccaa ttttgcaagg cttggaaaac ttatataaaa ataaacaaac
    11461 acgtgtcaag caggattaat aatcttctgt tgacaatgcc cccttttatc tcatatcttt
    11521 gccgccatga aaaaaatacg tcgcagtctc aaacccgcgg gaggttcaac gaccagagta
    11581 aaaccgcaac gggaaaatgt ttggtgcgaa agatacggtt attttatcga tctggatgcn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggtggtggaa cctcaaaggg
    11761 agggatttcc gccgctgatc ctttttaatt aaagtcaaag ccgtggagtg cctccgggac
    11821 cttcacggct tttttgtatc tacagattga agccgtgaat agatttcacg gctttagtcg
    11881 ttttagggag aatttttatg taccaaccga attttgcaga atttgaaaag ttagctcaaa
    11941 aaggaaatct gattccggtc tatcgggaaa tcctagccga tgtggaaacg cctgtttccg
    12001 ttttaatgaa acttcggaaa aaagaccatg tattccttct ggaaagcgtc gagggcggcg
    12061 agaaatgggg acgctattcg tttttgggaa cggacgccgg cgtggttttc agagtccaag
    12121 gcgatagagt tatcattgat gaaaaaggaa atgtgacaac ccgccaccat catggcggtc
    12181 ctttgaattt tctccgtgag ttgctgaacc gctataaacc ggtggcgctt cccggcctgc
    12241 cgcgttttta cggcggcgcg gtagggtatc tgggttatga gatggtgcgc tatttcgaag
    12301 aacttccacc cagtccgccg gatgatctga atctggatga tgctgttttt atcatcagcg
    12361 attccctgat gattttcgac aacgtgcgtc ataccataaa agttgttgcc tgcactttta
    12421 ctgaagatga aggatcgctt gaagcaggct acgcagcatc atgccggaaa attgaagaaa
    12481 tgattgaaat catttcagca tccgcatcgc acaagatagc cgcagcaaca gaaggtgacg
    12541 tcaattttca atccaatatg acctgtcacc agtataaaga cgccattgaa aaagccaagg
    12601 aatatattgc ggcgggcgac atcattcagg tcgtgctgtc tcagcgtttc gaaaccgaat
    12661 atttaaacag ctccattgat ttgtaccgcg cactgcgttt catcaatccg tcgccgtacc
    12721 ttttcttttt gaagatggac gatctgacgc tgattggctc ttcacccgag gtgatggtcc
    12781 gcaaagagga cgataccatg gagttgcgcc cgattgccgg gacaaggccg cgtggcagaa
    12841 cggaacagga agaccgcgcg ctgtccgatg aactgctgtc cgatgagaaa gagcgtgcgg
    12901 agcatgtcat gctggtggat ctgggacgca acgatctggg acgcgtggcg caaaccggca
    12961 gtgttcaggt caatcaattc atgatggtgg agaaatattc gcacgtcatg catctggttt
    13021 ccaatgtgcg cgcgcagttg accaaaggca aagatgcttt cgacgttctt tcagcaacct
    13081 ttccggcagg caccctaacc ggtgcaccga aagtgcgagc gatgcaaatc atcagcgaac
    13141 tggagaaagt caaacgggga ccttacggcg gagccgtcgg ttacttcagt ttcagcggca
    13201 atatggattt ctgcattacc atcagaacaa tgattctgaa aaacggcaga atttacgttc
    13261 aggccggagc gggcattgtg tatgattcca tccccgaatc ggaacatcag gaatcgctca
    13321 acaaagcccg cgggatgctg caggccataa agcttgcagc aggaggattt gaaatcagta
    13381 ccaatcaggc atgattagca taagaccatg taagctttca aaaattcata tcttaatatt
    13441 tgaaagcaat ttggtttaaa aacggccggc aggagaaata aatatgattt taatgatcga
    13501 taattacgat tcctttacat tcaacatcgt tcagtatctg gaacagatgg gcgaaaaagt
    13561 gaacacttat cgcaatgaca aaataaaaac ggatgacatc aggcgtcttc agccgagagc
    13621 gatttttctt tcgcccggtc cctgttcgcc cgccgaggcg ggagtaacgg ttgaggttat
    13681 acggaaattt tatcaggacg ttcccatcat gggcatctgc ctgggacatc agtccatcgg
    13741 ttacgcgttc ggcgccacag tatcgcgcgc cggaagaatc atgcacggga aaatatctca
    13801 tgtaaaacac gacggtcaaa ctatctttgc cggactgccc aatcctttcc ccgccgggcg
    13861 gtatcattcc ctgattgtct atccggaaac gctgcctgac tgtctggaaa tcagcgcact
    13921 gacggacgaa ggcgagatca tgggattacg gcacaaaaaa tatccggtgg aaggcattca
    13981 gttccatccg gaatccgttc tgacaccgca ggggaaacgc atcatcagaa attttttaaa
    14041 atatacaaaa taataaggag cgacgatcat gattaaagaa gcaatcgaaa aagccacgat
    14101 gaaaacggat ttgccggaag cggaaatgat ggccgcgatg gatgaaatca tggagggagt
    14161 tgctaccccc gcgcaaatag cctcgttgat cacagcgctg cgcatgaagg gagaaaccgt
    14221 ggaagaagtc tcgggcgcag cacgcatcat gcgccagaaa gccactcgta ttaatgcctg
    14281 ttcttcaact atcatcgaca cctgcggcac cggcggcgac aagctcaata cgttcaatat
    14341 ttccacgacg gcggcctttg ttgcagctgc ggcgggactg accgtcgcca agcacggcaa
    14401 ccgggccgtt tccagcggct gcggcagcgc cgatgttctg gaagcgcttg gcgtgaatat
    14461 cagcgccgac caggaaatag ttgaagaatg tgttcagcag atcggcatcg gatttctatt
    14521 tgcccccaaa ctccacggcg cgatgaaata cgccatcggt ccgcggcggg aaatcggcat
    14581 tcgaaccatt tttaatatgc tgggcccgct gaccaacccg gcgggagcta cagcccagtt
    14641 gcttggcgtt tacgatgcaa aacttacaga aatgttcgcg catgttttaa aaaacatggg
    14701 tacaaaaaaa gcgtttgtcg ttcacggact ggacggactg gacgaagcca ccattaccgg
    14761 agagacgcgt gtggccgaac tccgtgacgg gattgtccgg acatataata ttaatcctgt
    14821 tgattacttc ggacggctgg attccctgga atccattcgc ggcggcgatc cttcggccaa
    14881 tgcccaaatt acccgggatg tgctctcagg caaaaacggt ccctgccgtc atgttgtcct
    14941 gctgaatgct gccctggcga ttgttgccgg agaaaaggcg aaagacatca aggaggggtt
    15001 ggcgattgca accgactgca ttgacggcgg cgcagccgtt aaaaagctgc aaggactgat
    15061 cgaaatgagc aacagttagc gatactcgct gagcgcgaat gcaatgacgc gcgaagcgtt
    15121 agcggaacta tcccaggcgc aaagtaacct gaaggtcact ataggcacac tgaaacttgc
    15181 tgaacgtaaa aagactcgaa ttccagaaac gttgtgaact gaaattcgca aatcgaatta
    15241 tccaataacc aaaggctatt ggaaactatc ccaggagcag agcgacgtgg aaatgctcga
    15301 caaaattatt gaaacaaaaa aagaagaagt ggcgcaactg aaaaaagaaa caacagaggc
    15361 gcaactgaaa aaagaaattg ccggatttgc cccctgccgg gatttcaaaa aggcgttaac
    15421 gagcagcgac tgcaacatta tcgcggaagt caagtgcgcc tcaccatcac gaggccggct
    15481 ggtcactgat ttcgatcctt tgaggattgc gcggatttat gaacaaaatg gtgcggcggc
    15541 gatttccatg ctcaccgatg aaaagtattt ttccggacac aaaaattatc tgaagcaaat
    15601 aaggccaacc gtacaattgc cgttattgcg gaaggatttt attgttgatc ccctccagat
    15661 ttttgagacg cggataattg gagctgacgc tgttttgctt atcgtacggg tgttaggaga
    15721 aaaacttgct gatttcattt cgctctccgg ggaagttggc ttaagccaac tggtggaagt
    15781 ccactctcag gaagaactgg atttagcgct ggccgccggc gcggagatta tcggtatcaa
    15841 taaccgcgat ctggacacat ttattacgga tatcgaaacc agcgtcaaac tcaaaaagcg
    15901 gattccggaa aacaaccttg tcgtggcgga aagcggcatc cggaaaagaa aagatattga
    15961 attattgatc aaagcgggaa ttaagacctt tttaatcggt gagcatctcg tcacggcgcc
    16021 ggacatcggc aagaaactgc gcgagttgaa aggagagagc tgaagtgact caagtaaaaa
    16081 tctgcggcat agccagtaaa ggagatgctt tcgctgcagt aaattgcggc gcggatgcgt
    16141 tgggctttat tttttatccg ccctcgcccc gctatataaa accggatgat gcagaaaaaa
    16201 ttatccgtgt tttgccggat aaggttgtga aggtgggagt attcgtgaat gaacaaccga
    16261 aaaatataaa aaagctggtc gaaacctgcg gactcgatat gattcaactg cacggagacg
    16321 aatcgcccga cgattgccgt cttttcccgt cgcaggtatt gattaaggcg gttgagttaa
    16381 agaacgaatt tgatttaacc cccgcctgcg cctatcctgt ggcggccatc ctggtggaca
    16441 accgtcatgc cggacgttac ggcggcaccg gtaaaaaatc caactgggac ttggccgctg
    16501 gcctcagggg taaaataccg ctgattttat ccggaggact caacgccgaa aatgttgccg
    16561 acgctttgaa aacagtcact ccggacgctt tggacatcaa cagcggagtg gaagcatcgc
    16621 cggggaaaaa agatcatcag aagctggccc gtatctttga caccatccgg gccttcgata
    16681 caaagcatga aaattttcaa tccattttta aaaagaggga cacatgaaga atacattgcc
    16741 tgacaaaaac ggccatttcg gcattttcgg cggacgctat gctgccgaga cgctgatgcc
    16801 cgcattaatc gagctggaaa aggcttacaa aatcgcacaa aaggacaggg gcttcgagaa
    16861 agagtttgaa tggtatcaga gggaatatgc cggacgcgtc acgccacttt actgcgcaag
    16921 aagaatgtcg gaatctttac gcggcgcgaa aatttacctg aagcgcgaag atctgaacca
    16981 taccggctcc cataagataa acaatacgct ggggcaggcg ctgctggcgc gccggatggg
    17041 caagaagaaa gtcatcgcgg aaaccggcgc gggacagcac ggtgtcgcca cggcgacagt
    17101 cgcggcgctc ttcggaatgg aatgcaaaat atttatgggc gaagaggaca tccgccggca
    17161 ggcgcccaat gttttccgga tgaagaccct gggcgcggaa gtcgtgccgg tgaaaagcgg
    17221 caccgccacg cttaaagacg cgatgaacga agccatgcgc tactgggtat cttcagtgcg
    17281 tgacacattt tacgtgatcg gcacaacggc cgggccgcat ccttatccgg ggatggtacg
    17341 cgattttcag tcggtcatcg gcaaagaaac gaaaaggcaa atcatgaaat tcgaaggccg
    17401 atatcccgac gtgatgatcg cctgcgtggg gggcggcagc aacgccatgg gcactttcta
    17461 ccctttccgt aatgaaaaaa atattgccat gtatggcgtg gaagcggcag gtcatggcat
    17521 gaatacgtct gaacattcgg ccagcatcaa tggcggcaaa gccggcgttc tgcatggaaa
    17581 taaaacctat ctccttcagg acgcacatgg acaggtgcgc gatgcctatt ccattgccgc
    17641 cggcctggat tatcccggtg tgggtcccga gcatgcctat tttcatgcta cgaaaaaagt
    17701 caaatacgta acgataacgg ataaggaagc gattaaggct ttttcatttt tgtcgctgaa
    17761 ggaaggcatt attcccgcgc tggagagcgc gcatgcgatc gcctacgcga tgaaactggc
    17821 gcccaaaatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcgctggag
    17941 agcgcgcatg cgatcgccta cgcgatgaaa ctggcgccca aaatgactaa gaataaaatt
    18001 attgtgattt gtctgtcggg gcgcggcgac aaggacgctc agacgattat cgagaagggg
    18061 gttatgggct gatgaaacca attgaaaata aattttcgga attacgcgcc agaaatgaaa
    18121 aggcactgat tgtttattta acggcaggcg atccgtcgct tgatattaca aagcaactaa
    18181 tctttgctct ggaaaaatcc ggagtggaca tcctggaaat cggcgtacct ttctccgatc
    18241 ccacggccga cgggccggtt attcaggcgg cggctcaacg ctcgctcaaa gccgggacga
    18301 ctttgaaagg tgtgctgaaa atggttgccg acgtgcgcaa gacctccgat attccgattg
    18361 tccttttcgg ttacttcaat cccatctttg cctacggtgt gaaaaacttt gctcaagctg
    18421 cccggcatgc gggcgtgaac ggcgtgctgg ttgttgatct gccctatgaa gaagcaaagg
    18481 agctccgaga ttacacggat gctgtcggta tggattttat ttctctcatc gctcccacca
    18541 cggataaaga aagattaaaa aagattgcct cgaaagcttc aggatttctc tactacattt
    18601 ccatcaccgg gatcacgggc acagccgcgc caaagatcgg caatatcaaa acggaagtga
    18661 gcaaaatccg taaaataact aaattgccga tagccgtcgg tttcggaata tcccgacccg
    18721 aacaggcgcg agaaattgga cgttttgccg acggtgttgt catcggaagc gcggttatac
    18781 gtctgattga cgaaaacaaa aataacggcg acttgtcaaa gattctttcc gattacatca
    18841 gcaacattaa aaaagaatta cgataatgaa agcagattga tctcatcagt tttttcttgt
    18901 ttttcatgta ataaaatatt acgaatttgc acgttaattt aatttcgatc ttttccgttg
    18961 agcaaagcat tgagagggtt gcttttgaaa aatccccatc taaaaattct ttttctgaaa
    19021 tggctgccac cgaaacatgt catgaaatta acgctggtct ggttcttaca ggcaacggca
    19081 gttatcggat atgtcaacta tacatatgat ggatatttca tgcgcttgtt catgcattgg
    19141 atcattgtgt gcttgctgtt gatcgtatta atgattccgt ttgacttaaa atctttcaaa
    19201 attgttcctt ccgtcgtcag gcgaattacc ctggtgctca ttctgagctt tgttcattat
    19261 ttcctgcttt gtttttacgt tatgaccata ttgggctata aaagctggag cggtcctttt
    19321 acgtttgaat tattgagcgc ctattcggga catatcgatg tgctgctgaa tatttacgga
    19381 ttgtctgttg aagcggcgat cattattttc ttatttatat ggctgttaat attcgcagga
    19441 tattactatt acagcggtgt tgcggcggaa gaatttaccg caaatgaaaa tgacgcaaca
    19501 caacctcatt cccgatggcc ggttctggtc ttattcctcg tgatgatatt tgcaatcgtg
    19561 tattcaaaca gttacaaaag ctggcaaatg agcgaacctt tgcacatcgc ctggcttaac
    19621 gggcatggtt gtttgagaca agctccgcag ggaatattca acaaacgtcc tttgtccgga
    19681 gaaaaacagt caacaacatc agcggagccg tatgaaggaa taactccccg gccgcttgtg
    19741 cttatcacag tcgatgcatt gcgcagcgat caaatgggtg tgtatggggc tccccgggaa
    19801 accacgcctt tcctctcgca cttatggcgc ggaggaaaac ttcgtcgaat cgataatgca
    19861 tattcaattt gcaccacgtc gttttgcggc atgataggca tattgtcgtc caaatactgg
    19921 caccagttaa acgagccccc tgtgaatctg gccgatgtgc tcaaaaagta caattatcgt
    19981 aacagctttc ttttaagcgg cgacacgacg aattttttcg gtttacgcca ggtcttgggt
    20041 tcccatattg atcttttttg cgacggatcc attgaaccga aaaaatatgc aaacgatgat
    20101 cgatccgttt taggctggtt gcaaacatac aactggaaat cggcggaccg tacgtttctt
    20161 tatattcatc tgatgtccgc acacatcatc ggtttgcgcg atccgcgttt caaaaaatgg
    20221 ctgcctgaca aagaatggga tattccaaaa ctattttatt cgcagcgcgc gtatcaaaac
    20281 aattatcata atggtgttct tcaggctgac aacacgatca gacaaatttt cgagattctg
    20341 gatcgggaag gtatgttaca acgcgcatta atcgtcatta ctgccgatca tggtgagttc
    20401 cttggagaat tcgataattt cggtcatggt ttcgagcctt tcgaacccat ggtgcgtatc
    20461 cccatgctag tctatgatgg actgcaatcc aactatcctg aacgctcgct gtccagccaa
    20521 gttgatgttg cccctaccat gttatatgca attggcgccg caataccaaa agattggtcg
    20581 ggcattcctt ttcaacttgc agcgccgcgc aaggcggttt acaccgcctg ttatgacgtt
    20641 tcaggcgtcg tggctgatat cggcggacaa cgttttaagt ttttgcaaaa acggaaaggc
    20701 ggcaaggaaa tgcttttcga cctggataca caaaaggcag aatctatcaa tcttgccgca
    20761 aaaccggaca tgcgaaaaat tcttgcgtcg atgcgggagc ttaatcaatt aacgcaaaaa
    20821 tcaaaatagc gatgatgcaa aaaacaaaat ggtttatggc aagatttatt tttttaacag
    20881 cggcaatggg aactgcgctg tcgggcggtc tgctaggcta tgttttgtgt cctctgttct
    20941 catggtactt ttttaaagac ctaaatttta taaaatatca tcactatatc atccgccttg
    21001 tttttgcttt ttggcgtcag gttgtcgaac tgctctataa tccggattac cgggaaatgt
    21061 tttacatccc atggacagat ccaccaatca atgctcccga tcccaaacgt gttcgtgtcc
    21121 gcgcactgtg gcagcatagt gataaaggtt gcgggttgtg caacaattgc tgcacaagaa
    21181 gagcgtgtcc tcttcatgat atgaaacaca atcaatgcaa aagttatggt tcttttttct
    21241 ggcgttattt caattgcgga cgttatccgg aaaacaccaa acaaattcat tattacgagt
    21301 gcaaaaaatg ggaaaggtat aactgcctga gcgaaaacga ataaaaaatg ttattaatat
    21361 ttaatatgaa tcacttccgg gatatttcgc aggcgcaatg ccggaaaatt aatcgctctt
    21421 atcgggccag gaatacgcgg gcggacgatt ctcaaaaacc gctccgtccg gcataacttc
    21481 cacgtctttg ccgtcgatcc gtttgatttt agtcagtatg gccggtacat tgcgtttccg
    21541 gacgcgcgca atcacttccg ccaccgtttt gcaatttttg atattctcga gggtcatgat
    21601 ctgcggcaaa accatatcca gccggccctg ctcatattgc cttatcgcct cggcgggacg
    21661 aacccagaca tgatcggtca attcgacgcc gtcatgaatg acggcctgat cggacggaat
    21721 ttccgtcaca aaaaaacgaa catcatagcg ttggggaaaa ggttccggtg taatccagtg
    21781 cgaaaaataa taaagtctgt ccagaggcag aagaagctct tcggttgcca gcatttcaat
    21841 aaaacgcatt ttaccgctga tgagcgcttt gcggtgatcg caaaagcgct gccgttcatt
    21901 ttccgctcta atcgtcacgg gtgaaccgtc ttttttctgc gccagcaaaa tgcccacctc
    21961 ttcaaatgtt tcgcggatac cggccaccca tgccccgagc gcctgctccg ggtaacgcat
    22021 gtcggacagg attttggacg cggcaagacg atccatgccc acaatgaaac gttccatgcc
    22081 gtcggcaaaa tcatcatttt ccagacctcc gccggggaaa acataatacc ccggcacaaa
    22141 actgcttttg cggtttcgta aaaccatcag aacttcaatg cctcttaccg aagcatccgg
    22201 acaggaacgt aggagcacga cggtcgaagc atcggcggga ataactttca tattttaaca
    22261 cctttgaaaa ggacaaccgg ttcctcgtta caaattgaac acaggggccg ctgtactttt
    22321 gtctcttttt ttatttgccc gattgaatgg gatcccagtc gtaaccccag atggaactgc
    22381 ccatgggcat tttgtaactg cgccgatccg aataagacgg atagcgccgg ttgccgcctt
    22441 cttccggcag gaaattcgcg ggaaactgcg gtggcatcag gccgtgccgc cgggcttcct
    22501 tctccagcca cgcgcggtcg tcgggatgcg cgattgaaat cagcgcgcgg gcgcgttcgt
    22561 agccgttgag gccccgaaga ttgaccatgc cgtattccgt gcagacgtat tgggcaaact
    22621 gtgcgggcac gtccaccacc gatccttccg gcaggaaggg aacaatgcgc ggcgtgttgt
    22681 gtttgctgca cgacgtcatg gccgcaacac tgcgaccgcc tttggactgg gctgcaaaaa
    22741 tgatgaactg gaaaaatcca cccgtgccgg aaatgggacg cttgccgtaa aacgaacagg
    22801 tcatttgacc cagcaaattc acggcaacgg tgttgttgat ggcgatcatg ttgtcgacgc
    22861 gacgcagcac atcgagatta ttggtgtaat cgatatcgta aaccgccagc gcttgattgc
    22921 ggtgtacggt ttcgtaatac cacttcacat ccacgggaaa agcgaacgtc cagacgctct
    22981 tgccctgatc cagcctcttg tacgcgttgg tcacctgccc cgattcgatc attttaatca
    23041 gaccgtaatt gagcatttcc gtatggatgc ccagatattt gaaacccgcg tccgccatgg
    23101 cggccacgca cgccgaggga agcgatccga tgcccagttg cacgacatcg cggtcgcgca
    23161 tgatggtcat gatgtggctg gcgatttcct gttccatcgg ccttgcgaca atgccctttt
    23221 cattcatctg cggccacttg tatttttcgc agtccacctc cacccagtaa tcgatatcgt
    23281 caatgtgaat ggtattgaaa cggcaacctt tcgcccaggg gtaatcggct ctgacttcaa
    23341 aaacgatttt ctttgccgtc tgcttgaaga tgttgctgct gttggtccca taggacatgt
    23401 tgaaataacc gtgattgtcc ggtgcggtcg ccgcgttaaa ccaccagtcg ataccgcggt
    23461 ttttccgctc gccgttggcg aaccggtaat gatgcgccca gatgccgccc gcccagcccc
    23521 acggcgtcca gctggttacg tcgttatcct cccgcgcctt gcggttccag gggaaaaaga
    23581 aatgttcatg cacgcagtgg tactgctgca tcggatcgat ctcctgcaat tccggatgcg
    23641 gataaaagtt gccggcaagc cagaactcga tgtcgtgaag atcgcttttc cctccgccca
    23701 gacgcttggc gaccgcttcg gtgcaggcag tggaatcacc gccgaccgcg ccgtgattaa
    23761 tccaatcgcc ggacttgacc atttcggcaa tctgcttggc tgttcttttt ttatctcgga
    23821 tcttctgctg tatcggtgtc gtcatagcct tctctcttac tttatttaca ctgccattta
    23881 atcttgcaat gtctccatca accggctcag ggtgcagaca ttcaacacca agaaggtcat
    23941 tgagcctttt ttataatata aataacaagt tggcggaaag tcttacacta gccacggaat
    24001 tttagcaaga cgattcagcc tgattacttc attattgatg aacggacgtg acgactgacc
    24061 tcccggcgtg gtggcatctt gcaaacattg aacttcagat caaggaatat catttctgac
    24121 ttctgcaaat atcatataac agattgcatt tctgctcgca ggcctgtttc actccgatgg
    24181 ttgacggaca atcataattg catactgtct gatcgatctt gcatttcgct tcggtgcacg
    24241 acagacagaa cattgccagc atcgtgaata atacgattgt gaccgtttta cagattctgt
    24301 aatgcatatt cattcctcct tccatgacgt ctgtgtgaag tataacaaaa aagatgcaaa
    24361 aggggtaatt cggaattgct gtcccgaatt accccctcat tgttcacaca attttttagg
    24421 ctattcttta tacatctcct cgatacggtc ttggtaatta gccagaacga tcttgcgctt
    24481 gatcttgagc gtaggcgtca gttcgccgct ttcctggctg aatgcgtggt taatgagaga
    24541 aaatcgtttg atctgttcca cgcgacccag cggtttgttc aattcatcga tggtctcctt
    24601 gtatttctgg acaacctccg gataggcgat cagttttgtc ggatcggtct cgttgatatt
    24661 tttcgatttg gcccatttaa tcagttcggt gaaacagggc acgatcaggg caacgaggta
    24721 ttttttctgg tcgcccataa tggcgatctg ctcgatgagc ggatgcatga caaatgcgcc
    24781 ctcgatgacc tgaggtgaca catttttgcc gcccgcggta atcaaaatat cttttttgcg
    24841 atcggtgatc gtcagataac cgtcttcgtc gatggtgccg atgtcgccgg tgtaaaacca
    24901 tccgtctttc gtgagcacct gcgctgtgtc ttccggccgg ttgtaatatc ctttcatcac
    24961 ctgaggtccg cggatcagga tttcgccgtc ttctgcaatc ttgcattccg tgcgcggaaa
    25021 ggccggaccg caggtgccta tcttgatcgg cttgagatgc ttgtatgtgt tcagatgcgt
    25081 gatcggcgtg gtttcggtca gtccgtaacc cagatggatt tcaacgccaa tgatctgaaa
    25141 gaactcattg atggtgttgg ataacggcgc gccaccaacc ccgatcgtca ccatgcgatc
    25201 aagccccagg gccttgcgca acttggaata aatgagtttt tcgaaaagct tgtattgcag
    25261 attcaggaaa aacggcaccg gtttgttgta aagaatataa ggcagcgcct ttttaccgac
    25321 gcccatcgat gcgtgaaaca gcttctgttt actcggtgga gcgtttccca gctggctcat
    25381 gacgccttca taaatttttt ccaggacacg cggcacgtat acggccagcg tcggcctgac
    25441 gtctttcaag tcgcccagca gggtctcggc acctttggtg taggagatgg tgcacccttc
    25501 atgcatggcg gaattgtagc cgactgtgcg ctccaacgca tgggaaagcg gaagaagcga
    25561 cacacacgtt cccctggggg ggaacggatg atgctcgacg aattgtttta cgttggatac
    25621 cagattgctg tgggtcagca tggcgccttt gggattgccc gtcgtgcccg aggtataaat
    25681 tagggttgcc gtttcctgaa cgtcgatatt tctcaaccgt ttgtcgattt cgtcgagatt
    25741 gaggctggct ttaccgatct gcaagacact ggaaaacttc acgacgatcg gatacctgga
    25801 atcaatttcg tcgaaaacga tgatttcctg cagataatga agattatttt tttccttcaa
    25861 cagattgtcc acctggaatt tcccggcgca catacatatt tttgcccggg aatcattcag
    25921 gatgtattcg gcttccggcc cggagttggt cggatagacg gtgacgtcgg ctgctccgat
    25981 ggccagcgtg gcgagatcgg ctacggccca ttcaggccgg ttgttggcat aaatggcgat
    26041 tttatctgtc ggctggatgc cttttttcag aaagtacgaa gcaagtgcat cgacctgccc
    26101 gctgatatcg ccgtacgtgt actctttcca ctttttgttt tcatcgcgta ttctcagaaa
    26161 aattatgtcg cgggatttct ccacctgatt tctgaaaatg cctgggattg ttgtgtcgtt
    26221 gtaaggttta aattcactca taatatctct cctgtatttt atgtctgtcc cggcttcagg
    26281 tccggacgat ccgaacacca tcagccggct caggtaattt cacgttttga tttgaaaact
    26341 gcagtactga caaagctaga aggggaactg atagaataca aacagataag gtcaggtctt
    26401 gaaatacaag ttttcattca tgtttaactc ttatgatcat cgcatgatca taattaataa
    26461 tactgacttc ctataaacat tgcaagaaaa aacaggaaat gtcggcggga gcaaggttgg
    26521 acaaaaaaca ggggggttga tttttgctaa tataaacaat atattggtga tatatttcgc
    26581 cccgaagaac gtctttttca gattatcaga aatccgttta tttgctttat tttatgcctg
    26641 cttttacgaa tcaaccggct ttgacacgat tgttcgtttt ccgctcatgt ctgaacaaac
    26701 ggttaagacg attcatggtt tcaatcaaca gcgaaatgta ctttccttct ccttccaggg
    26761 tcagtaaacc atgcacgacg gcgcggttca gcgccttgcg tttgcccgct gccaaatcga
    26821 tcatgacttt accgccggat cgattatccc gccagatcag ggcaaaatcc gatggccggc
    26881 gaccccggac cgattgcacc ctgccgcgga taaattgaaa atagcgataa gtatcctctt
    26941 ccggggagcc catgataaat gaatagtcgt gagttgccag atgcgcacga aaatctttac
    27001 tgataagaac agccagccgc atcatgcagg caaaaccgta catcaggaaa gcaagccggg
    27061 ttttaaaaaa gattcttttt atgggcataa gcattcagac gcattgaatc gccatattga
    27121 aaaacgtcca atgtaaaatt ttttaaccgg gccccggttt tcaagcaaca aacaacggct
    27181 gcagcttcat ggcaaaagca atgtcgcctt cgattttcat tttaccggac atgaaaagat
    27241 taatgggaga ttctttcccg ccgctgatca tgttggcatc ctgcatggag agccgcaaaa
    27301 cggcgatcgg cttttctgcg ccgttgaata tggcggttat ttcggaaacg gtatcgtcgg
    27361 catttttgat ctgaaaaaca cccttcccgc ttaatgcggt caacacatca aattttttcc
    27421 tggtgagctg tttctgcatg gcgatcaaca tatcaatatt cttcatgtcc gccattttgg
    27481 acagggtatc gggagaaaag gcgatccgaa cgtgcggatt gtcgacagcg ccttcccgga
    27541 tgctgaaacc gacgccatcc tgaatgatat aactgtagac gctgccggat atgtccaggg
    27601 ttattttcag ttctgtccct ttcaattcct cctggtctcc ccgcatctga atgtattgtc
    27661 cggccaactt ggggataaac tccatgaaca actcctgaac attaactgta atcgtgaacg
    27721 tctgttccat aatcccttcc tccagagatt taatttgtgt gcttttttat caaaggcacc
    27781 cacttttttt ggattttttt tctgttgagg gtccgatcgc gcaatgttcg caggtgcatt
    27841 ggatagtcca acaaatgatt gaacgggggt tttcaaaaag cgtcattgtt tcgggaggat
    27901 gcccgcctgg caggcatcct cccggtttga agtgatttaa aaattctgct ggccgatgta
    27961 tctatgccgc agttgccttt tataatgcgt caactgtgtc cagccacgct cccagccgcg
    28021 ggaacatttc ggtcggcatt ttcttgccca tcgccaaatc gaaatggccg gttccgtcca
    28081 aaacatacca ctcgtcatac gccgttggcg tttttttcga tatgataaag tcgtaagtcg
    28141 cctgcggact caccagggat cccgtgctcg atgaaaaaac gatcatcggt acggtcatgc
    28201 gtgccatatt ctcgctatag tagtagtaac cgtctcggcc ggggttgggt gccgaaaccc
    28261 ctttgaccaa tttcgacgat agatagtttt cttttccgtt taagtagtgt tcacgaaggg
    28321 tattgttcag gcccatgtcc atgtaatgac cgaagccgcg tatggcagcg ctgccgacag
    28381 cataacgcac cgtccaatcc tccaggcagg ggtccatatc ctcgaccacc cagaaattca
    28441 gatagtcgaa gaagtcgctt atcgcagaat caccggcggc cgccttgttc agcagcccga
    28501 acatgccttc cagcaaaccg accagcggct tgctcggaag cacctgaatt aaattatctg
    28561 aaaggtaatc cagcggcaga tacaatctcg atcctacaat tgtccagaaa agaggggtgt
    28621 ccagaaggtt cggcagcgac ggcacgcctg cggggtcaat accgataaag ccttttacat
    28681 cctcattcct ttttttggca agatcagcgc tggctctgac atgcggcata tagccgaggg
    28741 aatagcataa tttatagtaa gctttctttg cccaggccga ggaagcaatg ccggattcca
    28801 tataggcgcc ctgcagataa tcataggaaa caagacctcc cgtgctgtgt ccgccgataa
    28861 acatgcgttt gcctgtcacc gattttacct tggcgattgc tgcgggagca tcctgcgtgg
    28921 cccaggtatc aagggtcgtg aggctgttgg cgttggttcc gtcgctcctt acctcaccgc
    28981 ggcctgtgcc tcgataattg acaaaccagg gatcataccc cttaagccag aggtagtgcg
    29041 ccagactgta ataacgcatt ttgtcggctt tgatgtacgg ttcatagtct gttttgtcct
    29101 cattcaacgc ccattcagga gcctgatcaa ccggaggaac ataaacgctg ctgtagacat
    29161 ccttcttatc gggaggcgta caggaaaggt actggttcat attcatggtg atgccggtaa
    29221 agatcagaac cggcgtagcg ctggtgttaa acttcggggt accggtcgtg tacggcgcat
    29281 agcgatacaa aaatatcttg ctgccgtcat ccgccgtggc gctgtagatg ccgctggcat
    29341 tcaggggaac tctggatacg gcctggccgg tcaccggcac catggcgatt cccaaaacgg
    29401 cgatcgccat cataatcaaa aggtttttgt aaaaaatggg tttacttttt ggacgtctgc
    29461 ttttagacca agccacctgc ttcattcctg tttccccctc ttttggattt ataaaaaact
    29521 tgtcaaaata agttttttaa ttcacgattc aagccccgcc gtacagcacc tgtacagacc
    29581 atttcgcgtg cggaaaaaat cagataatat gtgtttttgg atttcgtact gtatgagact
    29641 tttttgaggt tttgtcaaat aaaaaatgta attattttaa agaaatggat tgtggttcga
    29701 tatggattct tcatagattt acgaacaaaa caaaagcgga ccgggtgcgg atcagcaacc
    29761 ctgctcaaac gcaaggccgt gagttccggc aagtccaagg acagatctat taccgacggc
    29821 gtcgtcatgg gcgacgccgc tgcgacgagt gaatttaaga cacggattca tcaaggaagc
    29881 cgtcctcaat tgccattcat caatttaagg ccgggtgcgg attctaccgc ggtgccgcgc
    29941 gtattccacc atgcggccaa acgcaaacat ggcgcgctcc ggtgtgggat aacagggaac
    30001 gcccagcctg gaaaatcctt catggacatg agcaatttcc ctggcggtgc ccccatagta
    30061 actggcaaag accggcttgt ccggatgatt gcggaatagt tcggcaaacg cttccggaat
    30121 gtcgaacatg ctctccggca taagaacaaa aataatcaac agggcatcca tatccctttg
    30181 atccagcatg cattgggcaa tatggatcga tgtctttttc gaaccatgct gttcgatggc
    30241 cgaccaggta tcaatgggat tgcgcaccgg cgcccatgca ggaatcactt ctgccagttt
    30301 tttgagcgtc gcgggattca gttcgggcag aacaatgccg tattcctccg cggcatccgt
    30361 tgcagcgacg caacccatgc ccgtgatgga aaacacaccc atgcggtttc cccgaggcag
    30421 ggggagtttc tcaaaggcgg ataaggtatc gaaaaatgct tcgggattgt gtgttcgcac
    30481 gagacccgtt ctgcggcaga cagcgtcgaa caccgcatcc gatcccgcaa gcgagcctgt
    30541 atgcgacgca acagccgccg ctccggcttc actgcgtcct gctttcatca cgacaaccgg
    30601 ttttttcctg ctggcttttt cggcggctct gatgaaacgc cgtccgtcaa tcacgccttc
    30661 caggtacatg ccgacggtca gggttaccca atcctcggcc aggtattcca gaagatcgct
    30721 ttcgttaatg tctcccttat tgccgatgca agccaccttg gccagcccga agggcttggt
    30781 gtctgctatc cagcgcgccc agcctgacga aaaaaccccc gactggccga tgatcgatac
    30841 gccgcctgcc ttgattggtt caaggccaac gatggacgtc gccatgccgg cggagggatt
    30901 aagcgtcccg atgctgttgg gacccatgat ccggatatcc gctttgcggg cctgttcgat
    30961 aataattttt tgttcctcgg cgccggcttt gcctgcgtcc gaatacccgc ccgtactcag
    31021 aatgatgttg gtgactctct tcgccttgca gtcttccaaa gcctccggca ccgcagtttt
    31081 gggaatcaca atcatggcca aatccaccgg cgccggcaca tcgcagactt ttgcgtaaga
    31141 gggcagaccc aggatttcct tcatcgtggg attgatcgga taaatggcgc ccccgaaacc
    31201 ggccttttgc aggttgcgca gaataatgtt gccgcccttg cccggacttc gagaggcacc
    31261 gataatcgct atggaggccg gattgaaaaa gggaacgacc gtagcaggct cgggatgttt
    31321 gcgggcggat ctggcatggc tgccacccag ccgcacgaga gcatctacag ccacggcacc
    31381 atccggatac gcgatgaaag gatttacgtc tatttccaga atgtccggtc gatcggatat
    31441 taatcgggag agggatgaga gggcttcggc aatggcaaca acatccagag ccttctgaga
    31501 gcggtagccg tcgagcagcg ctgctcctct gaggcgcccg agcatcgcgc acgcaattgt
    31561 attgtccacc ggggcaagat cgatggcggt gtcgcggaaa atttcggtaa agatgccgcc
    31621 gattcccgcg aggatcaccg gcccgaacac gggatcacgc cttgccccca ggatgagttc
    31681 agtccccggc cgcgccatct tctgcaccag gaatgcatga ggaacatcgg aaaatctatt
    31741 gttcatgtcc cggaaagcat cggccagcga ggaggtatcc gcaatgttca gcgcaacgcc
    31801 accccggtcg ctcttgtgaa gtatcttctc ggaacaaatc ttgagcgcta cggggaaacc
    31861 cagttgattg gccgcaacgt caatttcctt atcatccctg gctaaaatac tatccataac
    31921 agggattccg cccttcttga gcagtgccgt cccgtcaaac tcgttgagtg ttatgatgtc
    31981 atttgtcaaa actaaaacac ctcctgattc cactgttttg aaacaaacgt tttcataatg
    32041 aacacacgcc gcgatatctt gtcaagccag agttcgtctt ttccggtgat ttcgaatgac
    32101 ttcaatgaat ctctacaaga ttttttcatt ctcattaaaa acgattttat aatttgaaaa
    32161 gtatttcgta atcttataag taacaatacg ggggcaagat gaagattaca tttttaggaa
    32221 ccaacggctg gtatgatacg gattgcggca ataccatttg cacgctgatc gaaacaaaaa
    32281 cggcctatat tcttctggac gccggaaacg gcatctataa ggcggacaaa tacatcaaga
    32341 aaaatctgcc ggtttacttg ctgctcagcc atttccacat cgatcacatg gagggtctgc
    32401 acatcctggc gaaattcaga ttcacatcct tggagattat cggtcagacc ggcaccaaaa
    32461 aaacgatcgg ggaatttttg tctccaaaat attctctgcc ggtcacccgg ttgccttatc
    32521 cctgtaacgt aactgatatc aatgaaggct ggcacaggcg tccttttcga ttccgggcgc
    32581 tgccgcttga gcacgcctcc agatgtttcg ggtaccggct ggagatggag ggcaaagtca
    32641 tcagttactg caccgacacc ggatactgta aaaactccga ggaactttcg gtaaacgccg
    32701 atttgctgat ctcggaatgc gcatttctca gcggtcaaca gtccaagacc tggccccaca
    32761 tgaacccgca actcgccgcc aggctggccg ttaaatcaca agcgaagagg ctcgccctga
    32821 cgcatttcga tgccgcctta tatacaacaa gggccaaaag agcaaaagcg cagatgcagg
    32881 caaaaaggat atttccgcac acgtttgctg cgtctgacgg attaagcata gtgatttaac
    32941 agcataatga tgattccgga tttgtcctcg ataatcgaat aatttaaaaa agtctttgga
    33001 atatcttctg taagcggcat cctgtttgtg aagatttgcc ttgcttcttt aagcttcatc
    33061 accttgttaa ttgtctgctc gttcgccagg ttcggggcaa gacgtaatgt gttgtatttc
    33121 ggaaatcttg acccgcactt tttaagcagt gctttgttca tcgcaaaatc ggaacccgga
    33181 cgatcgaata gccgcagaca tctctcggcg tcggagaacg ggaatcaatg aaactgtcag
    33241 tcttttttgg gaaatcccgg gaagggcata acggttcatt cagcgacttc gatacatgat
    33301 aacgtttaaa tacggttctt tttctgtacg cttcttcacg ccgctgaacc gggacgaatt
    33361 aatcttttat gatttcatga gggagtcatc tctctctgga caatggcctc cagttgttcc
    33421 attgcatcat ccatcgtgcc ggtatttagg atatatgcat cggcgagggc aatgggaata
    33481 gccgttccat aagcaatttc acgctggtcc cgctcgttaa agcccagagg agaatcatca
    33541 tctcgtcctc gcgaacagac gcgctccagc cgcagtcctc tgggagtcag gaccgcaacg
    33601 accacaccat ccgttttttc gcgaatgagt tctatttcct gccaggaacg catgccttcc
    33661 agaaaaccaa catcggcgcc ggtcttcaga atttcttcca gggcaagccg ggtgacgccc
    33721 aggccatctt caccccgcag atcatcggac acctcggatg ttgttgcggc atcgacttcc
    33781 aatccgcgtg ctttgacctc ggcccggaca atatcgccgg tcgagtaata tacgatgtcc
    33841 cgtgatcctg cataaatccg ggcgatattc ttacccgctg ccggcatgcc gacaatgata
    33901 aaaactttca tatctcccct cgcaaaagtg ttttagttta ttttctctgt cgtatctttg
    33961 tgccttcgct gatcgtatcg tcagggtatg ctatcacttt ttcgttttct tttaaaccgg
    34021 aaatgatctg agccgtcagt ccgtttctct gaccgatctc gataacgcgt ttgcgtgctt
    34081 ttccatcgtg ctcaacgaaa accgcccagt cttttcccgc acggaaaagg gcgcttgccg
    34141 gaatctgcag aacgttgtca tcttcccaga ttgtaaagtg cgcttccatc cggaaaccgt
    34201 caccgagaac gctccactgt tccggcggcg acgtaatatc gacaattacc agaacccgct
    34261 gttcctcaac gccaaggctc gatatcttcg taaatcccgc cggctccacc agacgcacgc
    34321 tgcccgtcag cggctcatca ttaccccagc gtttaaacag cacgtgtgtt ccttgtctga
    34381 ttttcaccgc atccgacgaa aggacttcca cccggacttc aagatttttc gggtcgccga
    34441 gatccatcaa cggcgcgccg atgttcaccg caccttcgct ttcatgataa attctaaaca
    34501 cggctccgtt caccggcgaa gatacggcaa cggcattgcc ctggcttctt acggctgaaa
    34561 aattccgcaa ggccactctg gcttgttcca gttcggaacc tgccctatgg acctccgctg
    34621 cagacgataa ttgcagggcg cgcgcttttt gcgcttcggc atgaatctgg tcaaactgat
    34681 ctctggcaat ggagcccttg tcatacaagg cttttaatct ttccctgcgc tgatccagat
    34741 acgcggcatc cgccgcggcc actttttcgc gttccacggc tgccttcaaa gccgcctcgg
    34801 cggcggaaac cgccgcacgt gctgcggcgt ggctgcgggg atcgagcgcc tgcgactgca
    34861 ggggttcgag tactgcaaca atctggccct gttgcacatt gtcgcccact ttggcatgaa
    34921 tgcgctgcag gtatccggca gtgggcgcag aaatggtaaa gcggtccttc aggcgggtgc
    34981 ggccttcttc ttcaatggta acctgaagcg gccctcgcgt gacaaaaaca atatccacat
    35041 cccgcgtctt gggaagaaaa ccgtaaatga gcaggccggc aacgaccgcc atgatactga
    35101 gaatgaatat ttttctgcgt aacacgtgat tcatgaaatg atcctctgac ccatctgagt
    35161 ttttttattt tattcccggg ttttgagcac ctcgacaaga tcgaggttgt tgagcttgtg
    35221 acggacgata caaccggata tcaccgccga tacgataacc accaacgccg cctgcgcata
    35281 cgttttaagc tcgatcatca tcggtatgcg gtatagatcg gttgtcagag cgtgagccac
    35341 ataagcgcat atccagtatc ctataataaa tcccaaggga atggcaacaa gagttataaa
    35401 ccccagttca cccagaagaa tataggcaat ctcgccccgg gtatatccca gcacgcgcag
    35461 agaagacagt tcgcggctgc gttccgccag ggaaattctg gcgctgttgt acaccacacc
    35521 gaaagcaatg gaacaggcca tcactgttgc aataaacata aagaagagca tcgccttggc
    35581 catgatttcg taaatgctgc gaatctcgtt ttgccggacc acgatttcgg ctacccgtgg
    35641 catttgcaca aacaactgga aaagtttttc tctgtgggtc tcatccgtta ccagataggc
    35701 gccggagatg gcatcgcctt cgttgatcag gcggttgagc gcttccaggt tcatgtaccc
    35761 catcaccccc agatactgtt tagccatagc aacaaccgga acctggcgga cggcccggga
    35821 cccttccagc gcctcgacca tcagcagatc tcccgctttc actccgagaa tctctcccag
    35881 ataatccgtc aacagaatac cggacggcgg catggaaata ctcttcagat ggttgtccag
    35941 caaaatgttc aggtggctgt ccggctggat gccgttgatg acggttttat aactgcgatg
    36001 accgaatttc atccgcaccg gaacactccg gaatgcttcg ccgctgctga taccttccat
    36061 gcgtttcagt tcatataccg cccggtagga cgtcggttcg acaaagctta cggtcatatc
    36121 ttccttctgc gacaggacga attgaatgtg caccatgtaa tcaatacagt cccggaaaaa
    36181 accgctgcag atcatcgtcg cgcaggcggt tgcgatgccg atgatcgcca gcacggtctt
    36241 gaccggcttt cgttcaatgt tgcggaagat aatacgcgac ggctgcgaca gccagcggcc
    36301 gatgccagtt ttctcaatca gcgataccct gtatttcacc ggcgcttccg gtcgcatcgc
    36361 ttctgccgga gtctgccggg cggcattcca caacgaatgc atcgtcccgc tgagcgcagc
    36421 ggcaacactg atcacgacga ccgctatcat cacttgcgga tggaggatat aatgaaaaaa
    36481 cgggaaccgg taggtttcca tgtacagcgc ccccagcatt ttgccgaacc agataccgcc
    36541 ggcaatcccc agcaacacgc ctgtggcaat aatcatcatc accatcgtcg cgtaatgaac
    36601 ggcaatgctg agattattat agccgaatgc tttgagcgtg ccgatctgct cgcgctgcgt
    36661 gctgatggtt ctgctcatga caatgttcag cagaaaggcg gcgacacaga tgaatatcgt
    36721 ggggtaaatt ctggaactgg tcttgagctg ccggaattcc tcactcaaca gccggtggga
    36781 aatctgatcc ttgcgtccgt aagcgccaaa gccgccgtac cgtcccagaa tgttgtccag
    36841 atcccgcaca acatcactca atgccgttcc cggataaacg gttaaaacaa catcgttgaa
    36901 cgcgccttcc atatcgtagg ccttgctgat agccttgcgg gtcatccata atgcgccgta
    36961 ccgtttgaaa tccggcgtca tggcgcccgt cttcatcagc aaaatgaatt ccggagaaag
    37021 aacaatgccg gtgacggtca gtctcttcca tttcccgtta atgaccgccg caaacttatc
    37081 gcccggattc aggccatgcg cctgcgcgaa ggtttcgttc atggccactt cattatcccg
    37141 tgcgggatcg ggaagacgtc ctttacggat gtaaagacga ttcagcagag gatcaccgtc
    37201 atcgggaatg gaggttatcc tggcagtcac cgattccttg aatccccgga tatccagctt
    37261 ggcctgggcg caaaccctgg tttccacctg atggacgcct tgaatgtcat tgatcttata
    37321 tttgagggtt tccggcgccc gctttaaatt gacgaaaaca tcggcaaaat tgtattcgcg
    37381 gtaaaacttg ctgcgggtaa aatcgagaga atgcatggtg ctgataaaca tgatgaaggt
    37441 ggctacgccg ctcatcacca ccagcgtaat ggcaaacacc tgccctttca tcagccacaa
    37501 ttcccgccag agttttcggt tcagcgtttt catcatctta ccactgcagc tcgctggcgg
    37561 atttcttcac tttgttgatt tcaacactgg caatatggcc gttgcttaaa tgaatgacgc
    37621 ggtcggccat gcccgcgata tccacgttat gggtaatgat ggcggtagcc gtgcccagtt
    37681 cgcggttgat acgctccagg gcctggagca ccacaatgcc tgtttgggaa tcgagcgcgc
    37741 cagtcggttc gtcgcacaac agcacagccg gttttttgga aatggcccgg gcgatggcca
    37801 cgcgctgctg ctcgccgccg gaaagctgcg caggaaaatg gttcaacctg tcagacaagc
    37861 cgaccaggtc aagcgcttgc tggggcgcca tgggatcggg agcaatctcc gtaacaactg
    37921 aaacattttc ccgggccgtc aggctggaaa tcagattgta gaattgaaaa acaaatccga
    37981 catgaaaacg gcggtactcg gtcagttcct tttccgtggc tttggtcagt tcttttcctc
    38041 tgtaggacac cacaccgccc gtggctgtat cgaggccgcc catgatgttc agcagggttg
    38101 atttgccgct tccggacgga ccgagaagaa caaccagttc gccggcatag agttccatgg
    38161 tcaccccgcg caaggcatga acggccacct cgcccatttg ataaactttg gtaagatcct
    38221 gtacctggaa aaatttttct ttctgctgat tcatccgact caccggttgt ctgacgggct
    38281 gcgggttgac ttataatgtg caggaataat cttaatatgt atagctgatg gcggcgccgg
    38341 cggagaacct gtccgcatat tgtccttcaa cgttcagacg aaaacctttg gcaagcggga
    38401 tatccagacc cgcaaaacct ccggcaatgg acgtgttctg aacagaataa cgaccggcag
    38461 catatcccca gtccgacaca ttggaggaaa gagacagcgt tccttccgag taatataaat
    38521 acggccccgc atacatcgtc gagccgcagg gcagcgtcgc ctgcaaggcg acaccgaaat
    38581 gaacatccca gagatccttc agttgaacat gcttcctgcg ctcaacggta tcggaaaaat
    38641 cattcaaata ataggtcccc tggacaaatg cgccgactcc cagcttcccg ctgaccgggt
    38701 aaaaaacctt tccgcccaga gttgcaaaga cctcttcgct ttctctgaaa acgtcgccta
    38761 ttttcagatc ggcaatcccc agccgggcat atatttccca ggcattgtca gcaccgtagg
    38821 ccgcctgcga ataaatctca ttctgcctga tcttcaaccc gttgcctttt tcatatgtat
    38881 cctggtgata ccggtaaccg atggcggtat tcaatccgcc gtcctgacgg gaaacggtct
    38941 caaaggcgct gaacaatcct gcggcgcttg ccgccggacc ggttgtaaaa aaaactaacg
    39001 ccgtcagaac aaaaaccatc caatatggtg cgcaacgtat ttttttcatt taatccacct
    39061 tgtccggccg gaccgtctcc ggaaacggtt ctgctgccga tgatccggag aaaatttttg
    39121 cctttctggc tttaatttgc ttgtaacggt actgaatgta ggcgtcgcga aaagccacat
    39181 acggatcgat ggccgcattt ttcagcgctt cataatcgcc gattctcagg gatgcggcgt
    39241 tgaccttatc caggacattc acacccaggc gggccgacca gggattcagg taggcaacag
    39301 gatccagaaa ataattgccg gctatgctga cggtatcccg gggggtggag ggaccgaaga
    39361 ccggccagac aatataaaaa ccctgtccca cgccccagac gccgagagtt tgtcccagat
    39421 ccgtgttctg ctcctgtaaa ttcaactcct tgccggaaga cggatccagc agaccgccga
    39481 tgccccagac ggtattaatg gtaaaacgtc ccgcttccgt cgctgcacct ttgaaatcag
    39541 cctgcagcag acagcttaaa aatcgtgcgg gaaatccgag attggaaaag aaattatcca
    39601 cactgatgcg cgcgggttcg gggacaacgg ctttatagcc ctgagctacg ggcttgaccg
    39661 cccagaaata cattttatcg ttgaactcat gcattgccct gttgaaaggt tccagaggat
    39721 cggcgatgga cactttttct tctttagcat tgtcccccgc atagtcatcg aattcgtccc
    39781 ccgtgtattc ctcttccatg ggatcaacca cagcggccgc ggcgaattga tttgcgggcc
    39841 ggtccgggga gactgtcagg accacaggcg gcggagatgt ctgctccggc ggcagatcaa
    39901 caaatgcatg agcgctgccg ggtgcgcccc ccgacagcat ccatataaac gatacgagaa
    39961 ataccaaacg agatttcatg tcatccgacc gtatgaaagg ttatcagccg gcgtgctgca
    40021 acacagccgc agggtcagca ggttttttaa tgctgcttaa ccttgtttct gagaacttcc
    40081 agcaattttt ccggtttgtc cttggacaaa atatcgttga actgcgtccg ataattctga
    40141 accaggctga cgttttccac cacgacatca tatactttcc attttccgtc ttttaaaata
    40201 acgcggtaaa aaacaggaat ttccttggaa gaggtgacga tcttgctttg cacttccgcc
    40261 tgggttgccg aaagcgtggt ttctttataa aagacgacct tttcattggt atagctgagc
    40321 actttatcgc cgtacgctgt ctccagcacc tgctcaaaaa gattgacaaa ttccgtccgc
    40381 tgggcaggcg taaaattatt ccagttgcgc gtcagggtgc gtttggaaaa ttcaatttca
    40441 tcgaacatct gtttgtatag aacccggagt ttttcttttt tcagttcctt ggccgactcg
    40501 ctcttgagct tgggatcgcc cacgataccg atgacctcgt tgacgcattt ctgaacggaa
    40561 tccagcgccg cgcccgcata aacaggcaga gaaaacatta caatcaccag gatgatggct
    40621 gtttgggtaa aaagtttttt catgaatttt tcctccctca acgtcgtaat aaccgatcta
    40681 ttgtttttta acatcaccaa aagcgtattt gctgatcagc gtctctatgt ccaacggtga
    40741 ctgcgtttcc agaataattc caccgggctc cagcagagag cctccgccgc ccggatcgat
    40801 gctgacatat ttgtctccca tcaatccttc cgttttgatc gaggcgatgg catcgtcata
    40861 aatcttgatg cctttttgaa tatttaattt caccacagcc cgttgattgt cctgatccat
    40921 ggcaatttgt ttcactttgc ccacagtcag acccatgacc tttacggaac tgtcgatccg
    40981 taaaccgttg acggacgtaa atttagcttc aaggggatag gacgcggctt ccaaaaaaga
    41041 cacatttccg agtttcacgg tcatatatcc gatacagaga agaccgagaa acacaaaaat
    41101 tcccacgaca gtttcaatct cgtatttttt catgtgctta ctccttgacg gcacattgca
    41161 gccgtccgat ttcttttcga tgagctttgg ccagttttac aattgaatca atttcagcga
    41221 ccggtgtccc ttccgccata ccggctttga ctgaaaaatc aacgcacctc gtctgaggat
    41281 cgtttttttg agcttcgctc catatctcac tcatgccact tttttggaaa tcctccataa
    41341 agtctttgac aatgctttcc gtctcggcaa tatcggaaaa aggaagcact gtcacgaatt
    41401 cattgatttt acgacgggtg gagaaaccgc cgatggcatc aaaatgttta tcgatgtact
    41461 ttcccatcga atggatggtt tcctgcgccc tgtcgtaacc gatggcggaa acaatcgaat
    41521 tcatatccga taaatcaaac acaaccacac aatagccttc gtcggacttg cggctttgta
    41581 actgggaacg gtgcaaaacc ttaaattgtc ttctggaata aagaccggtt aattcctttt
    41641 gcaaaccttc cagactgcgg atcacttcat cgttgaaagg atgatcaaaa ttactcaatt
    41701 cctccggtgt tccctgaaag acgatgcttt tgtcatagag ggcgagaatc cggttggaaa
    41761 taaagtacac atcgggaatt tcatgactga ccatgaccgc cgtaaaatta aattttttct
    41821 gatactgggc gatcatgctc aaaatcgcgt ttttccggac agggtcctga ccggttgtcg
    41881 gttcatcgaa caaaaccacc tggggatcgg taatcaacgc ccgtgccagc gccgctcttt
    41941 tttgcatgcc tccggaaagc tccgaaggat atttctgtgc ggcttctgtc aattccgtct
    42001 gttcgattct ggccatgacc cggtcatgaa tttcgttttt tttcatggcc gtattttcca
    42061 gaagcggcag cgcaatgttt tcataaaccg tcatggaacc gaacaaggca ttgctttgaa
    42121 acatgtagct gattttggac atggcttcgg ccagttctgt tttcttcatg tcgttgatcg
    42181 gttctccccg gaaaagaatg gttccttcat cgggtttgag caagccaatg atgtgcttga
    42241 gcagcacgct cttgcctgaa ccgctcaggc cgataatagt ggttacttcc ccttcgtaaa
    42301 tctgcaaatt gacctgatcg agaacggttt gttctccgaa tcgtttggtg atatttttga
    42361 attcaatcaa cggtatgttc atatcttttc tataccatca ggagtgacgt tacgacgtaa
    42421 tccgcaacca gaatcaatac acaggaaata accaccgctg aggtggtggc cagaccgacc
    42481 gccttggcgc ctttgctgtc ctcgcgcata tgaacaaaat atccctgata acagcacacg
    42541 gtcgcgacaa tcaccgcgaa cacaagcgat ttgacaaacc cgttggtcag gtcttccata
    42601 tccacgtagg accgaatgct gtgtgaataa acgccggcat tcaatcccat cagagccacc
    42661 gcgctgacat aaccgccgat aatgccgatc agatcgaata cagtggttag caggggaaaa
    42721 ctgacgatcg acgcggtgat ccggggagtc accagatagc gaaaaggatc gatgcgcatg
    42781 gtttccaaag cgtcaatctg ctcggaaatc cgctgcacgc caatttcggc tgttattgcc
    42841 gatccggccc tggccgtaat cataatcgcg gtcagcacag gccccagttc ccgaatcagc
    42901 gacagcgaga ccagcgttcc cagcgatcct tccgcgccga actgcaccag cgcatgatag
    42961 gattgtagtc cgagcaccat gccggtaaac agactgacca gcatgataat cgtcgtcgat
    43021 cgcgccccga tataataaat ttgctgaatg accttttgaa actgcttggg atgaaaggtt
    43081 ttgagtaaag ccagcaccag aaaaatagtg gccgctccga tgctgttaat ccaccggatg
    43141 acattccgcc cgatcaaggc aaatggtttg ctcaccgttc ccctgaacct ctgtgtgtct
    43201 ccagtcctat tctgcttgta atcatccaaa gctatcaaca gatttcttcc ctttcattgt
    43261 tcccggaatg ctagcgtaaa aattttacat atttaaagca tttcgtcaat gatttcctaa
    43321 aagacgtccg ccagaaattc ttttataatt ttggcgattt ctttaggttt ttccatggga
    43381 atcagatgac cggcatcggg aatcaactgg taccggccgt cccggagaag cgatgctgcc
    43441 ttgggcagat cgataaactc tctgttggag cttttttccc cttccagaat caggacggga
    43501 caggtcaact tttccagcaa cggccaggga ttggtgcttt taccgcccat aaaaagagcc
    43561 gcttcgttgc ggggcgcaca ggtaagcttc aaatcgccgc cggcctgttt ttccatgccg
    43621 tattgcagat aaaattgcaa catctgttcg tcccagcccg caaaaagcga ttttgatttc
    43681 agatactcca gcgcggcatt ttcatccttc cagccgtttt ttcttcggat ggacaaagag
    43741 gccaggggat gatctttgag ctggatatcg atggtataaa aatcttccgg aaggaaaatg
    43801 ggttcaatca gaatcatccc gcggggctgc agaccaaaaa gcgcggaggc aatcgtcacc
    43861 accgtggcgc ccatcgaatg cccgacagcc agattatcgc tgatattctg aacgctgcaa
    43921 aagctgatca ggtctctggc aatcacatcc caggaaagcc cgccgctttc gggatcgcaa
    43981 tcgcggtaat tacatatata tggcgcccac gcctgcctcc tgggcggcgt cagctcctcc
    44041 aggatgggat gccaaagcca cggtagaaaa ccggtggcgt gggcaaaaag aatttgcgga
    44101 gtctctccct cgtaataaag atacggtaaa tgcgctccgc cgatatcgtg gtggttcaga
    44161 gacggaatat ccagaattct ttccgtttgg gtcatagtcc gcaccgggga aaaggtcgca
    44221 tgtcaccggt gtatgtctcc cttcggggat tcatacgccg tttatacgct tttaaaaata
    44281 tttaaaacac aaataccgcc gctgcaatca cggtggtgta tttgttgtct ttgcaaattg
    44341 tcgactgggt cacatttagc gttcgaacga tcttgccgct gattttaaaa atttccttct
    44401 tttcgtccca gctctcatca acattaaaat cgatgcccaa tgtggaggcc agcatggccg
    44461 ctgcaagatc ttccgcgtaa tcgccggcct gtttttcatt ctgaccgaaa gcatggtgtt
    44521 cgctgatgta accgtaggat gatttatcgg cgggaatggc gcaaccgacc gaagcggcca
    44581 tgagtcgttt gggttcgttc gtacagcaac ggctcataac gcagtacgtg atggcgcccg
    44641 gcgtcaattg tttcagcccc tgagttttgg agatcatttt acagcgtggc ggaaagatgc
    44701 tggacacctg caccaggttg catttctcta ttccggcatc acgcaaggca cgctcaaagg
    44761 aatgcaattc gtctttgtgc gtgcccactc cttttgtaaa aaaaatcttt ttgggcacaa
    44821 aactatccat ggttcatctc cttcttctac ccgtaaacgg cgccgtattt ttaaaacttt
    44881 tcgccctgcc cgtcgaaaat ccaaaagttt gtatagcagg gttcgatccc ttattccata
    44941 aattatttat aaaatacaga aaatttttcg ccgtttcatc ttgcccaaaa catgaaaaaa
    45001 gactatacaa tacgccagaa taaattgaag ggatcgggca tgaaagtaaa atacgtctgc
    45061 gttgttttag cgctgttttt agtgtcctgt tatttgcaac ctcccgatac cctccaggac
    45121 gtaaaagatg tcaccgacct gccccaggat cacaccgccc gtctggcgga cgtgccggca
    45181 gacaaagtcc ttctgacccg tgatagtcaa actgcgatga atgaacatta cgacaaaacg
    45241 tttttttccg tgtggcacca tcctgaaccg gtgcacgcac tgccggaaag attgtctgcc
    45301 attttcacac aatttgccgc ccatccgggc tatggcgaaa acaagagaaa gcattccacc
    45361 gcatggttga aaaaacttca gcagaacgcc tggctggaaa attatcccaa tacgctgcag
    45421 ccggcgatca ccgtccgatc gacgaacctg cgcatgctgc ccacgaacgg tccacatttt
    45481 tataaaccgg gcggcgacat caacggctgg ccgtttgaca atcttcagat atcttcagtg
    45541 gcggccaaca caccgattct gatatgccac atcagcaggg accggtcatg ggccctggtg
    45601 gaaaccagct ttgccttcgg atggattccg gctgaggatt ttgcacgcgt tgacgatccc
    45661 tttatgcaga catggcaaac ctcccgctat gccaccgtca cccgggataa aatcccgatc
    45721 gtcgacgcaa acggcaattt cagtctccgg acctctatcg gacaaatgtt tcctctgatc
    45781 accgagatgg cggataaact cgaaattctc atcgcctcgg cggacgcaaa ccatcaggcc
    45841 attatccaac gcgttttcat ttcgcgggag atcatgaaca tcaaaccgtt gccgtttacc
    45901 tatgcaaacg caaccagaat tgccaatgaa ctgattgacg aaccctacgg ctggggcggt
    45961 ctttacggaa accgcgattg ttccgcaatg acgcgggatt tttttgctcc tttcggcatc
    46021 tggctgccca gacattcggc ggatcaggca aaaaaaatcg gcatttatat tgacctggag
    46081 gctatggaac cgcaagaaaa agaaaaagtg attctggaac agggcattcc ctatttgagc
    46141 ctgctgtggc gcaggggaca tatcatgctt tacatcggcg aacgaaacgg gcgggcactg
    46201 atttttcaca atgtctgggg actgagaatc acagacgccc gcggcgagga aggacgcaag
    46261 atcatcggca aggccgtcat cacgaccttg catcccggca aagaattaac aaacatcgat
    46321 cccgatgcct tgctgatcaa aaatctttcc gccatgacga tactggcgcc ggcaaagtaa
    46381 atagcatgtc ttcccgttga cgccgctgtt taagaaaata ggtcataagg gacgacgtat
    46441 taaacaacac cttcactgaa aatggcatcg tacaaattta aaattctgct tgaatatgac
    46501 ggaaccggtt accgcggctg gcaaacgcag aaaaacgccc gcagcgttca ggaaa
//
LOCUS       DAONQZ010000014        43721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_952_length_43721_cov_18.791394, whole genome shotgun sequence.
ACCESSION   DAONQZ010000014 DAONQZ010000000
VERSION     DAONQZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 43721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43721
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_43721_cov_18.791394"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..4228
                     /gene="hrpA"
                     /locus_tag="PLR13_04140"
     CDS             170..4228
                     /gene="hrpA"
                     /locus_tag="PLR13_04140"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HQC18555.1"
                     /translation="MQSIRQDIRQIRQNLDSVMIADFYNIVRQLKNIEAHGARDEKSA
                     FRKLGYLKRIADLSTTRYRGRKQNSPAVQFPQNLPIITKKREIIESIQKHQVTIITGE
                     TGSGKTTQIPKMCLAAGLGLRGMIGLTQPRRLAAVSIAQRIASELGEECGQSVAYKIR
                     FEEKSSAEPFIKVMTDGILLAETVRNRDLLAYDTIIVDEAHERSLNIDFILGYLRTLL
                     SRRKDLKVIITSATIDTEKFSEAFNKAPVIKVSGRMYPVEVRYRPVDPDKEEKGETTY
                     IDEAVACVEQLQSERRYEDILIFMPTEQDIRETCGLLTKRCSGLVLPLYARLSGSQQR
                     LIFAAAEQQKIIVATNIAETSLTIPGIKYVIDAGLARILEYNPRSQTTGLPIKPISAS
                     SAEQRKGRCGRVQNGICIRLYSQEEFSQKPVYTSPEIMRSNLAGVILRMLELNLGAVT
                     SFPFIDKPQPKSIRDGIDILRDLGALTRDNSAEETNQYMLTETGWKMAEFPLDPRASR
                     MLIEAQKENCINEIAVIAAALSIQDPRERPYEKADEASKAHTQFAHPESDFLTYLNIW
                     NRYHGSLESLTSQSKQRKFCRDHFLSYKRMIEWRDIYHQILDIIDGKSKTKSRKHHRI
                     EINQDIADRIHRCVLSGYLSNIARKKEKNFYNAAKSREVMIYPGSGLFNRAGQWIVAG
                     EISMTSRVFARNVGNIKSEWLEKLGGDNCRYTYAAAHWEKNRGQVVALEKVTLFGLTI
                     VESRPVAYEKINPEEAKSIFIREALVTGEVARRIPFLEHNLSLYERVQTMEEKLRRRG
                     IVADEETIANLYEQRLPVLSDIRSLQNLIKEKDSDDFLRFREEDLIAREPDAEEISQY
                     PDRIKIGDAAFNCRYNFEPGKNDDGVTVKVPLGFISKAATANIDRHLPSLLKEKAVHL
                     LKSLPKSIRLKLPPPAKIVELLVEDKSNLDKPLPQALSQLLLDKYKIKVPKDDWATEK
                     LPDYLNVRYSVIDEKGNEIKESRDIHLLQKELTGTVSTSAMDKIRGEWEKEGITGWDF
                     GDLPQQIPLRGTHGLIGYAYPALTISDDSIDLRLFADEKQSAFHHLHGIAALYDIHFA
                     DKMKQLKKNVALTGAMKTSATGIGPPKQLEQSIINRVRKDLFYKPWRRQEEFIAHAGC
                     LNSQILPYGQQVLIAIEPVLKACADTCTVIGKLMTKNRNNNPVLNFIKDIQTEISRLV
                     PVDFPELYTFERMKELPRYCKALALRAERGNLNLSAAQRKMQDVLIYSQKLQQMVNGR
                     VQPSLFDLAVVKKQSSPKDPTEPKGTGFKEIILLKENITIDCPDEKKTMVEEFFWMIE
                     EYKVSLFAQELKTPYPVSPKKLNQLMEDIEKY"
     assembly_gap    4304..4403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4721..5245)
                     /locus_tag="PLR13_04145"
     CDS             complement(4721..5245)
                     /locus_tag="PLR13_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HQC18556.1"
                     /translation="MKSKSDVLKYLELAERQARDKKKKSNMKTYAYKYLELAEKHAEK
                     IAARWVKDVRNNTRTQTYKKLNEKQLVDQCTEFYQQFSKMFIYEKITDDVTKYFQKYA
                     LECYALDIPAKEAIYALILMRRNIWLYAEFQMIFNSAIAKQESLDTLNRTILLFDYAV
                     YEVTREYLELMKTT"
     gene            complement(5284..5904)
                     /locus_tag="PLR13_04150"
     CDS             complement(5284..5904)
                     /locus_tag="PLR13_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQC18557.1"
                     /translation="MPKIELPPTKSSLRKLKEDLAFAYEGYDLLNQKREILAIEIVRK
                     IDVIRKIEAEFQDIVNRLYLAYRQAAVDMGSEAVTLQSCSEMRSYYLHMNLTKLMGLK
                     LLNFTLDLKNNLPAFTLPPTTSAYDNAKEQSRKALHIVVQYATIMKSVIMLSRELKKV
                     QRRVNALEKIFIPQHEEAKKYITDRIEEMEREEIFVKKLIRQRQQR"
     gene            complement(5904..7295)
                     /locus_tag="PLR13_04155"
     CDS             complement(5904..7295)
                     /locus_tag="PLR13_04155"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQC18558.1"
                     /translation="MTMEIEYTNLSGISGPLIFVDGIKGVGYGDIVEIRDASGDLRIG
                     QTLEVDEQRAVVQVFEGTSGLSVKETRVTFVGRALEIPVDREMLGRVFDGLGRPVDGA
                     PQVITDRMMNVNGLPINPWSREYPRDFIQTGISAIDGMNTLIRGQKLPIFSGSGMPHN
                     RIAAQIARQARILTAEEEFTVIFAAMGVKFDVARFFIDSLEESGVLTHSAIFLSLADS
                     PSIERLVTPRSALTLAEYLAFEEGMHVLVILTDMTNYCEALRELSSSRGEIPSRKGYP
                     GYLYSDLASVYERAGRLKGGKGSITQMPILTMPSDDISHPVPDLTGYITEGQIVLERE
                     LFNKGIYPPIAGLPSLSRLMKDGIGEGRTRKDHGQVAAQLFASYAKVKRIRALAAIVG
                     EEELSDLDKIYLKFGTAFEEAFLRQAYHENRSIEQTLEIGWKMLSLLPRDELYRIARE
                     DIQRYYMSETEKT"
     gene            complement(7306..9069)
                     /locus_tag="PLR13_04160"
     CDS             complement(7306..9069)
                     /locus_tag="PLR13_04160"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQC18559.1"
                     /translation="MDEILKGNVISVNGPIVKVVGLTGIKMFDIAEVGQERLIGEVIR
                     LAGEITIVQVYEDNTGLKPGDDVISYGRPLSVLLGPGLIANIYDGIQRPLVGISEVCG
                     SYIKKGIKLPPLDVNKKWDFQPLVKTGEDINEGTLVGEIKESPLVVHRVLVPPGIAGR
                     LTAIVNAGPYTIEDDIFEIDTGYGKYNGKLAAYWPVRKPRPSRNRKKPFMPLITGQRI
                     IDTFFPIAKGGTAAIPGGFGTGKTMTQHALAKWCNADIIVYIGCGERGNEMTDVLTDF
                     PKLIDERNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRDMGYSVAVMADST
                     SRWAEALRELSSRLGDMPAEEGFPPYLGTRLAEFYERAGLVETLSGSNGDITIIGAVS
                     PPGGDFSEPVTQHTSRFVRCFWALDKILADARHYPSISWIDSYTEYLVDIEDWWKNLD
                     SEWLTTRNEVMNILLEDHRLEQVVKLVGSDALPPSEQYILFCAEMIKNAFLQQNSFDS
                     QDKFCSPEKQLKLLKCLLVLYQKGMTLIQEGVPVKDISQSDVVSEMVRLKSAVPNKEL
                     VEIDDYLKRLEQELMSIKSSS"
     gene            complement(9095..9700)
                     /locus_tag="PLR13_04165"
     CDS             complement(9095..9700)
                     /locus_tag="PLR13_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18560.1"
                     /translation="MDNTTLENSIREEASRAITAIREKEALEMRQRDEVFSMEMEDFR
                     RQAEAETESHIRQEISKLENKALLERKKMKLQNAEQFINRIVDEVMKNIRNDRLYKQF
                     LLEAIRDGVKISGADVDIKLSNEDIFLKKDIVAAIAAMDGRKKVSITEDSKVQWGGCL
                     IRDKETGRIFNHTLERIYFRKSLLIRQQVMSLLMRKAEGNQ"
     gene            complement(9710..10015)
                     /locus_tag="PLR13_04170"
     CDS             complement(9710..10015)
                     /locus_tag="PLR13_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQC18561.1"
                     /translation="MEKIYIIGDMHTVSAFRLAGVEGMVSTADSASVRLEEIIKKRDA
                     GIVLMTNDLAAALQERIREINLNRQNPVVIEIPGMDDIGGLRMSAMNYISEALGIRL"
     gene            complement(10228..10563)
                     /locus_tag="PLR13_04175"
     CDS             complement(10228..10563)
                     /locus_tag="PLR13_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HQC18562.1"
                     /translation="MIKNKLFKHFVFAVFTGFIWFALLPTVINAAENVQVNSGANWMG
                     LAYIGAGLAVGLACIASGFAVARIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLI
                     IAIMILNKL"
     gene            complement(10565..12451)
                     /locus_tag="PLR13_04180"
     CDS             complement(10565..12451)
                     /locus_tag="PLR13_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQC18563.1"
                     /translation="MFLKSDIRHITIALEKAYYSDVYLALGQAGIIHLARFSEKDSGT
                     DSGLQKEEALTREIISNTEYALNALMIAPEESEIFMPAVDKSGDLIFVSRIKKTIEQA
                     VRLRAKIREKENIIIRQMGYAEALSKMAIDPVAIKKARLVKILFGTVENMTDDVPPNS
                     RFMLNKINNYVLGICLPDDYPALFRFLKNYGFIDLSDDVTPVSLESLKKRREGLKRRR
                     DMMDAYLDRLKTEQGRWLQNLNKSYHHYEEILKAMRMSLFSAKALFITGWVDIRNKQR
                     LLKILEKICGNRFIFSERKDPDAPVHLRNMKLFKPFELIVKTMGIPSNREIDPTPLTA
                     ITFVLMFGLMFGDLGQGLVLLLCGIMLKKYGQKKIKENLKQAGGILVACGFSAAICGL
                     IYGSVFSSEHLLPALWFHPTANIMTLFAATILIGILFIMTGLLVNIINNILNADYTEA
                     LLDKRGLVILVIYAATVLFAFNYQRSGQVPAIWEISAFIMLPLTLFALRGVIGPAFFH
                     QESPHSASEYIIETVMEIVETGLSLFANTISFIRVGAFALSHAGLSIVTYTLAGIADP
                     GLQSAGAILIIITGNIFIIGFEGLICGIQSMRLEYYEFFSKFFQGDGVVFSPFTLKAQ
                     ASEV"
     gene            complement(12460..13410)
                     /locus_tag="PLR13_04185"
     CDS             complement(12460..13410)
                     /locus_tag="PLR13_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQC18564.1"
                     /translation="MRNYADQANLHARIYAMKGRLLSLQDYAVMAREQRTFAPKSSSV
                     QDLIDAKENLFREQIAPVIALVEAYDKYTPVFLAYLRQFETHNAKVLLAKAAGKDILE
                     QWYDISPFASLDKSLLNKNLSMDDVTSLLTNTYLGADFKPSSSYRRMQMNLDISSARN
                     IYNASSLISDQARKEFQEMILRRIAVQMVIRSYRLRVYYHWSEKDTRLYLEKINNRLN
                     IHAESQIHSIDEDLNQHIETLRKSSGQEPSVIDIEQHLEQIYYLWLSSIFYRDFHSIY
                     GVIAYLGLLLIQIKNLFRIIDGRRFGFSADAILSKIICKE"
     gene            complement(13416..13724)
                     /locus_tag="PLR13_04190"
     CDS             complement(13416..13724)
                     /locus_tag="PLR13_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18565.1"
                     /translation="MDDVITRIVDIEKQCSADVEATQLEYGKNIEAHKRFLEAKKTRE
                     RAEILSAENKRQSQAVEDAKRNIEAVSADFITDMENLFRDQGLNEAIKKDIVSILLGG
                     "
     gene            complement(13899..14825)
                     /locus_tag="PLR13_04195"
     CDS             complement(13899..14825)
                     /locus_tag="PLR13_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18566.1"
                     /translation="MQSAAGAEIDGSFIVCPLLSEQILAMKENAVLNKILKAVRIGER
                     SGAKVVGLGALMGVAGKGGQRIAEQTPAAITTGSSLASAAILETLDRAAAMRNVDLSK
                     AKVAIVGATNAIGQNCALGFLNRVDTVFLLARNADRLAELKEFLNAQKSKTNIIDQGP
                     ALEDVITEADLVIFTTSAIEVPSAATAKNLKKGAIICDIPSPRNIAREICDQRKDILV
                     IDGAVIEPPPTAQLGLKLPIKDGYIYACMAETMILAFEGQTQDDFSTGFRPDLHKVAR
                     IKALAAKHGFNIKFTSFGVPVQNIDKSLFSRL"
     gene            complement(14991..15425)
                     /locus_tag="PLR13_04200"
     CDS             complement(14991..15425)
                     /locus_tag="PLR13_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQC18567.1"
                     /translation="MKILAVNISQKKGGKKNNIGCGLFLENLGLESDAHAQADIIRQV
                     SLLAKESIEKIRAKGLDVQYGDFAENLTTEGIDLPSLPVGTRLQAGANVLLEVSQIGK
                     VCHNRCNIFYTVGDCVMPREGIFAKVLIGGEIKINDEIQISK"
     gene            complement(15453..16514)
                     /gene="mnmA"
                     /locus_tag="PLR13_04205"
     CDS             complement(15453..16514)
                     /gene="mnmA"
                     /locus_tag="PLR13_04205"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQC18568.1"
                     /translation="MGDKVLVAMSGGIDSSVAAWMLLNEGYNVAGVTMHFGALTEGDG
                     KRSCTSAAIDDAKKVCACLNIPHYVFDYASRLGNEVIADFISEYQKGRTPNPCVRCNR
                     HLKFGSLLDKAQELGFNYLATGHYAAIEKNEGGYCLTRPKDKKKDQTYFLYAIPYERL
                     KNILFPLAQLTKDEVRNLAMKNSLPVTAKQESQDICFVTQKNSQTFLLERVKDIAPGP
                     IVDVRGNVLGRHRGIIFHTIGQRGGLGISHPTPLYVLSIHPGNNRIVVGDKKDLMGKA
                     LIASGINRLAKSWPQQMHAKIRYRKKEALCEVREEKEKLKVIFAEEQEAITPGQSVVF
                     YDHDCVLGGGIIEKVIPFG"
     gene            complement(16587..17006)
                     /locus_tag="PLR13_04210"
     CDS             complement(16587..17006)
                     /locus_tag="PLR13_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQC18569.1"
                     /translation="MKISTRTRYGVRLMTALARNYGKDPVFLKNIAKEENISEKYLSL
                     IIIPLRGGGLVNSVRGAYGGYNLNKDPSEITLKEVVDVLEGDRFLVDCVNNPAACPRV
                     TTCASHDVWAVVGEKISETLSSITLDRLAKMNQKKEK"
     gene            17293..17853
                     /locus_tag="PLR13_04215"
     CDS             17293..17853
                     /locus_tag="PLR13_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18570.1"
                     /translation="MNIKGSSFVTTKAIITEAFGEERWNAFHAGLTQKDGFFNNMIMS
                     VTLIPVEKMIVFFNELCREFFNNDKTQYAMFGKSGAKFVLSPTGPYKSYMLAKDARQF
                     VELNMPKIWSTYFDGGTFTSNFDNNTAHITITGIPVKNNYFEELVMGFNQQAFKIFGE
                     KTTAVKVSSIAAGDDDFRFQLKLKDS"
     gene            complement(18095..18472)
                     /locus_tag="PLR13_04220"
     CDS             complement(18095..18472)
                     /locus_tag="PLR13_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQC18571.1"
                     /translation="MLKKIEAIIREDKVNDVMCALKEIGIVGMSMFEVRGHGRQGGVQ
                     LVGRAGTYQVNLLPKIQLNIVLSERNLEAALETILKAAYTGETGDGIIFVTPVEDVIR
                     VRTRERGEGAMIYPGDIDERRKK"
     gene            complement(18533..19756)
                     /locus_tag="PLR13_04225"
     CDS             complement(18533..19756)
                     /locus_tag="PLR13_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQC18572.1"
                     /translation="MDLQISAGDTGWILVCCSLVLLMTPALAFFYGGMVRRKNVLSTL
                     TLSYIFMSLIGVQWVLFGYSLSFGPDIGGLIGGLDYIGFQGVGAEPNAAYSRTIPHEL
                     FAAFQMMFAVITPALITGAFVERVKFKSFLMFSLLWATLVYNPLCHWVWGQGGWLKQM
                     GVLDFAGGTVVHIAAGFSALAFALVIGPRKGHGSVPMEPNNIPLTVLGTGLLWVGWFG
                     FNAGSALAANGVAVNALVTTNTSAAAAGLVWMLLSWLDGRPSTLGIATGMVVGLAAVT
                     PASGFVTPLAAMVIGAVSAPLAYYAIRFRERRKLDESLDVWACHGMGSTWGTVATGLF
                     ATVAVNADGANGMFFGNAGQLAIQITAVLVTILFSFGMTYILAKVLHVSIGLRVSPME
                     EEVGLDISSHGERSY"
     gene            20111..21655
                     /locus_tag="PLR13_04230"
     CDS             20111..21655
                     /locus_tag="PLR13_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HQC18573.1"
                     /translation="MTAPLIIAMSQGKEITLLPQMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGVPVFMADVKGDLSGIGAAGVASEKLLKRLEGIGVKDYQPRANTTVFWDVYG
                     KSGHPVRATISDMGPLLLARLLNLNDTQSGVLQIVFKIADDNGLLLIDLKDLRAMVQF
                     VGDNAKKFKTEYGNVSAASIGAIQRGLLTIEEQGGDKFFGEPMLDISDLIQTDSEGRG
                     VMNILAADQLYNNSKLYSTFLLWMLAELFEQMPEIGDPEKPKLVFFFDEAHLLFSDAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLAQLGNRVQHALRAFTPRDQKAV
                     QTAASTMRQNPAFDAEQAITELGVGEALISFLDEKGRPNIVERCNVIVPFSRMGAMTP
                     DERLAAIKASTIYGHYEKTVDRESAYEKLREAQQEEETPEEKPAIKVERSAAPAKSEE
                     EKDSGGMGDALSKMIFGSKGPRGGQREGMVQAAAKSVARSIGSSLGRQIIRGVLGSIL
                     GGSRGR"
     gene            21991..25245
                     /locus_tag="PLR13_04235"
     CDS             21991..25245
                     /locus_tag="PLR13_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18574.1"
                     /translation="MIEIDMRIIFAEYGLSVLLCLIVIVSLWWQNRKRAPEIILWLID
                     YILQFAGIMLIALRGILPDFMTIVVANLFIVGGTVILYEGLCRYVGQESRQKHNYVML
                     AIFTSVHIFFTYIAPHMGLRTINFSVALFYICIQGAWLMLYRVASPLRQATKITGIVF
                     AAFCIVNIIQIIINLIKTPSDNAFVSGAAGVIAVLIYETLFFALTFSLFLLISRSLAI
                     ELENELTRRMRTAEELRQNQEKFAKAFQTSPYAISITQMKDGKFLDINDAFVNITGYS
                     REEIMKESSMGLNLWVDKEDREEMVSILRQGLPVVRKEYRFRKKNGEILTGLYSSQII
                     NITEEPCLLSSINDITEQKQAEWTLKERIKELNCLYGISAVLDEPGISLNDIMNKAVK
                     LLPAAMRFPDITGARIILEETVFQTGLFKETPWQLTEKITVNGKPEGQVNVCYPAERF
                     QTDEKPFLDSERQLLKAVAEQLGHFIEQKRAEEQIQRNATRLTGLLNIMQHRTQTTQE
                     FLDYALDEVIRLTESKVGYIYLYNENSRQFVLNSWSKDVMKECTIPNPQTRYELAKTG
                     IWGEAVRQRKPIIVNDFPADHPLKKGYPEGHAPLFKFLTVPVFDQDQIVAVVGIANKA
                     RDYDETDVLQLTLFMDAVWKSVDLKMGEAKLRKSEERFKQLAEVFPETIFEADAKGNL
                     TYANEHGLKHFGLTEQDIAQGVNIFDFVSPNDRKAAWEKIQNRIQGTQKGYLEYEALK
                     KDGSPFHALALSVPIAVDGVPVGIRGFILDITKRKQAEEALRKSEENYHFIADHTADH
                     IWTMDMFMRFTYSSPSVIKTLGYTADEFMARKIDEFFTPESLVDAQKLLAEELEREND
                     PTADPNRSRTLPSHLRHKNGHWVWLESSLTFIRDASMKPIGVLGVTRDITERKKSEEQ
                     IKHLATHDLLTDLPSLRLARDRMVSAMNMARRYKKSAAIMFLDLDGFKNVNDTLGHDA
                     GDYVLKQVAQRLLSCVRETDTVARVGGDEFLIIATEIHHPDNAAHIAEKVIQTVSQPI
                     MINGEQAVVGTSIGIALYPEHSEEMDRLIKLADEAMYKVKNAGKNNFRFVNDVN"
     gene            complement(25487..26080)
                     /locus_tag="PLR13_04240"
     CDS             complement(25487..26080)
                     /locus_tag="PLR13_04240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18575.1"
                     /translation="MTVKPFNKKHVCPWWLCFTFDNPMRRIFHDPVTILGSYVRRGDT
                     AVDIGPGMGFFTIPLAKLVGPAGKVIAVDIQPRMLSALAARAQKHGVAAIIRTHLAEP
                     DSLALREKADFILAFWMAHEVPDLKKFFSEIRKLMKPQGWFFLVEPLAHVSRKDFLQT
                     IDLAKQSGLLMKECPKIRMSQSALFTPDKIERHEQPL"
     gene            26267..27652
                     /locus_tag="PLR13_04245"
     CDS             26267..27652
                     /locus_tag="PLR13_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQC18576.1"
                     /translation="MSPESISQTLTGLRAFFDAGKTQDVDARIARLKKLKEGIRRYEP
                     EIMASLYDDLRKPLFESYAGEIGILYTEIDHVIRHLPSWSRSRRAATPLIHFLSRSRI
                     DQKPYGVVLIIGPWNYPFQLVMAPLIGAIAAGNCALIKPSEFSPATSATIEKMISELF
                     DPGDVTVFQGDAQATQKLLEEKFDYIFFTGSTAVGRSIMTAAARHLTPVTLELGGKSP
                     CIVHEDAALQYGIKRIAWGKFFNAGQTCIAPDYVLIHRKIKADFTEKMIRTVFSFYGD
                     DPMRSPDYARIINERHFNRLLELLKSGRILFGGRYARKDLYISPTIIDRVSPEEPLMQ
                     EEIFGPLLPLIEYGDIDEAIAFINQRPQPLALYCFSEDKEIQEEVLQKTSSGGGCIND
                     TISHIGSQELPFGGIGASGMGAYHGKAGFDTFTHRRGILFRSNLVDIPLRYPPYRDRV
                     SILQLLFRLIR"
     gene            27714..28184
                     /locus_tag="PLR13_04250"
     CDS             27714..28184
                     /locus_tag="PLR13_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQC18577.1"
                     /translation="MAEKTIIILGKSVKNFTLADQFGQDFMLSRMTGRRVLLSFHPLA
                     WTPVCTRQMQSLEKNKKVFDKLNTIAVGLSVDSVPSKAAWAKAIKVKHTKLLADFWPH
                     GGVAKSLGLFRDQNGFSQRANVILDEKGKVCFVTVYPVADLPDINEIIAFLKNA"
     gene            28334..28621
                     /locus_tag="PLR13_04255"
     CDS             28334..28621
                     /locus_tag="PLR13_04255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HQC18578.1"
                     /translation="MITLIATLKVKDGNMDEAIQVLKEIVPQVKASEPGCLDYIPHTV
                     RGDDKTIIFYERYSDKDALKLHSANLMKNMEKLFPLLEPGMDVKTCYEIIR"
     gene            28688..30430
                     /locus_tag="PLR13_04260"
     CDS             28688..30430
                     /locus_tag="PLR13_04260"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQC18579.1"
                     /translation="MDVRRTINALKDVNIAKMFTTIARLLHFRLTWTRRDLDYLPAGL
                     GPKFVSARRAAAMIPDGATLTIGGFAATGRASIFYWALRDAFDRSGHPRNLTVIGACP
                     QGGRGKTPGMIEELDAPGIITRYIVGHGETAKALLQLADDGQLELHTMSQGALAFLIE
                     AQARGLASIQTSVGLDTFLDPAVGCGSAVSPGAPNNFITAKNGKLEYRIPPIDRALFL
                     APYADKDGNIYFKDAALILDYKDAAAAARLNGGKVIVSVSKIIPRDNSQIAIPADNVD
                     AIVVNPRNEQVGGILQTQFMPHFTAGSSEVDHEAVKLMRLINTFLDITPYRGKVVDIL
                     ARLCADTFVKTTGPGAIVNIGIGIGEEVARMLYESGLYRDITFTSEGGAYGGLPASGV
                     YFGAAISPQRILSTAEIFHIYEERLDTCVLGFLQVDSAGNVNVSHRGPKIIDFVGPGG
                     FTDIVEYARTIIFVGNWMDEARYGLKNGLVRVKKPGVPKFVDKVDQITFNARKALKAG
                     RKVLYVTTVGVFKLTEEGLELVQIMPGINIEKDILQNSTARFIIPGSPIPEVAPEIVS
                     GKRFELKFDKKDLA"
     gene            complement(30508..31011)
                     /locus_tag="PLR13_04265"
     CDS             complement(30508..31011)
                     /locus_tag="PLR13_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18580.1"
                     /translation="MRKIFAIFISLFFIASLTLAVTGCSKIEEDSANLQKAAAELKAQ
                     AEAKKAEAEMKAQADAQQAEADLKAQAEAMKAEAELKAKEMAKKATVKVKQVKKDVEK
                     VANKVANKVATDVKSAGQEVKEAAKETVVDVKSAGTEAKEAVKEKAVEVKSAVNDAAT
                     DVKNAGQ"
     gene            complement(31192..32421)
                     /locus_tag="PLR13_04270"
     CDS             complement(31192..32421)
                     /locus_tag="PLR13_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18581.1"
                     /translation="MISIFSTLRIALRALWVNKMRSALTMLGIIIGVSAVIIMLAVGT
                     GVSRKISSQISSIGSNLIIIMPGSTTQGGVRMGGGSQSTLTTGDAEAILRECSAVAAV
                     APMQFGTAQVVFGNQNWSTGIQGTTPGILEVKDCGLSAGRNMSDQDIRSATKVCLLGQ
                     TVVDNLFGSMDPLGQVIRIRKIPFTVIGVLESKGQSLGGQDQDDTIIVPLSTAQKKLF
                     GRTIPGMVRSIMVKARSSEELSVAERQITDLLRQRHRIGPNREDDFTVMNLTQVLEMA
                     EQTANTFALLLGAIASVSLVVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRIQF
                     IVEALTLSMIGGITGIIIGGTASFILSNVFEIPSVVSLPSVLLAFCFSGLVGIFFGFY
                     PAYKASLLNPIDALRFE"
     gene            complement(32418..33110)
                     /locus_tag="PLR13_04275"
     CDS             complement(32418..33110)
                     /locus_tag="PLR13_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18582.1"
                     /translation="MTLIETRQLVKTYDVGDEGVHALENVSVKIDKSEFVAIMGPSGS
                     GKSTFMNIIGCLDQPTAGDYFLDGQTISGLSRDDLAAIRNQKIGFVFQGFNLLSRTSA
                     LENVELPLLYNHVPARQRKEESLKALKMLGLEERAYHHPNQLSGGQQQRVAIARALVN
                     QAPILMADEPTGNLDTKTSIEIMELLVQLNRDSGTTIILVTHEPDIADFSKRIIRFVD
                     GHVVSDEEVQKS"
     gene            complement(33110..34294)
                     /locus_tag="PLR13_04280"
     CDS             complement(33110..34294)
                     /locus_tag="PLR13_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQC18583.1"
                     /translation="MKKKIIVISVLLIAAFAAGILFYVNRDGKKAVYKTEKISRGEVK
                     SVVTATGTVNAVTTVSVGTQVSGTIRTLFADFNSPVKKGQLLAQIDPATFRAQVDQAR
                     ANLLSAQANLEKAGVAVVDARRTYERNKELFSQNFIARSDLDTAETALHAAQAQQKVS
                     EAQVAQARASLRMAETNLQYTNIISPVNGTVISRSVDVGQTVAASFQTPTLFSIAQDL
                     TQMQINASVDEADIGQIRAGQDVSFTVDAYPDTDFSGKVSEVRNAPTTVSNVVTYDVI
                     IKVDNPELKLKPGMTANVSITIETRPDVLRVPNAALRFKPAEIGEKGVSAKELQDIKG
                     TKVWVLENNQPKPVGVTLGLSDGNFSEIVKGDLRTGQEIVTDSLNKKGSSTTQRPPPR
                     FM"
     gene            complement(34291..35565)
                     /locus_tag="PLR13_04285"
     CDS             complement(34291..35565)
                     /locus_tag="PLR13_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQC18584.1"
                     /translation="MQKKIMLLFLIVLIPFAAASEPAGGTASYDLDQCIAVALDKHPS
                     LKASAGDIRARESRVGQARSKYYPWLDVSAGYQKIGPTTPPGDSIDSYDQYSTGINLG
                     VTLFDFGKTSANVAIQDLNVNASRADYDDVRAQIILNVKKAYYTLLQNQRNRDVAVDT
                     MQQFQQHLDQANAFFRVGTKPKFDVTKAEVDLGNARLNVLKAENAVRAARITLKDVMG
                     IPENADFTIVDNLSFQKQAWILDDILNKAYAVRPDLLSIIARREAAERSIDLARKDYL
                     PTLSGSAGYGFSGRDFPLGRGWNAGASLSFPLFSGFSTNYQIDEAEANLAILTANEET
                     VRQAIHLDVQQAYLNVQDTAEQIAMAEMTVVQAQENYDLASGRYRSGVGSPIEVADAT
                     IMLNNARAGLNTALCNYRIAQAVLEKSIGAKP"
     gene            complement(35583..36218)
                     /locus_tag="PLR13_04290"
     CDS             complement(35583..36218)
                     /locus_tag="PLR13_04290"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HQC18585.1"
                     /translation="MVLASGKTNSKTRTAIIEAAGKIFAEEGYLKATVRDICSSAGAN
                     LAAVNYYFGDKKGLYLSVLKYYQEMAFQAYPPDLGIRASQKPEEKLKIYIRMFLKRIM
                     DDGRPVWFGRLLAREFSEPTCALDVLVNETIRPSFQLLTEIVADVLGKTPREKMVRLC
                     SMSIVGQCLYFRHSQPIMTRIYPEATFGAEQIDEITEHIASFSIGGLRKAQ"
     gene            36432..37424
                     /locus_tag="PLR13_04295"
     CDS             36432..37424
                     /locus_tag="PLR13_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HQC18586.1"
                     /translation="MNTKNMLRYISALTVIAAAVYFFYLQFKKNADAIGAWKFSINPL
                     EMSLAVIFGSCALLVGPLVWRLFVNSYLKKKLNYTESFVLYCTSAMFKYIPGKIWTYA
                     AQIALLSSKGISKVALLYINLVSFICLLFVAVPFFLYYYFIYVQEMSLGATLLILTLF
                     MLMDVVFIIANHTIVNYLIRPINRLFKLNIKPIRTDKIIFVYVQMLYSLACVLLGIAL
                     YFLARGVNMPLPFSQIIAMMATICVSLILGLLAFFTVGGLGVREGAMFFMLKQFSNIE
                     AALILPVISRFLITLVELLMAVTAIIIGLKYGYFHDIEKVKPAADSALPRESAG"
     gene            37461..38030
                     /locus_tag="PLR13_04300"
     CDS             37461..38030
                     /locus_tag="PLR13_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQC18587.1"
                     /translation="MMTFTDLLKNRRSIRDFQDKNVPMNLIEEILQESTLAPSASNNQ
                     PCRFSIVHCRKTIKALSDESKANLLEDYAGNKKSLLPQYIDMLKDENFNVFYNAPCVI
                     FIIGSKSVKSLDVDCALAASYIMFSAVERGLGTCWVNLGSNIRDPQSRALLAIPDGCR
                     IVAPIILGYPKTIPPVSERHAPEILRVIS"
     gene            complement(38166..>38446)
                     /locus_tag="PLR13_04305"
     CDS             complement(38166..>38446)
                     /locus_tag="PLR13_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18588.1"
                     /translation="CGYGPRYLHSTGQLHKGDAGNGLFIQLTQDFSLDVDIPDNIGEE
                     KSSLTFGALKAAQAKGDRQALTEAGRRIIRIHFKKDPVDGLKTIVNGL"
     assembly_gap    38447..38546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38547..39599)
                     /locus_tag="PLR13_04310"
     CDS             complement(<38547..39599)
                     /locus_tag="PLR13_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18589.1"
                     /translation="MTETRIDYSLGEYQAAVKSALDKMCRDNVIERIRSKDYTLWKFR
                     PDEIVNRLGWIDAPAETLAKINDIRSVVDALQKDKISDIVLIGMGGSSLAAEVFGNIF
                     GSKPGYPRLHVVDTTDPVFISEVTQHLNLEKTLFLVSSKSGSTLEITSLFKYFYNLCL
                     KKFGGEAGRRFIFITDEGSPLIKTAESISARHIFLNNPDIGGRYSALSLPGIVPAALI
                     GIAVETLLQNVSSNLEALMSAGASLGAALGMLANQGRNKMTFVLPPHWKHFGDWLEQL
                     IAESTGKEGRGILPVLNETLLDASGYGKDRIFVIFQNKNEEEPSQMETLKKDGHPVIT
                     VKINDDYELGQQMYIWE"
     gene            complement(39741..41798)
                     /gene="tkt"
                     /locus_tag="PLR13_04315"
     CDS             complement(39741..41798)
                     /gene="tkt"
                     /locus_tag="PLR13_04315"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQC18590.1"
                     /translation="MSDKNELEKQCIDAIRFLAADAIEKAKSGHPGMPLGAAAAAFTL
                     WTKHLKHNPKNPQWPDRDRFVLSAGHASMLLYALLHLTGYDLTLDDIKTFRQWGSRTP
                     GHPEYKHVPGVEVTTGPLGQGLANAVGMAIAEAHLAARFNGEDQKIVDHFTYVMAGDG
                     DMMEGVAGEACALAGHLRLGKLIVLYDDNKVTLAGSAALSSTENIEQRFKAYGWQVIK
                     VADGNDVAAIDKAIKKAKREAEKPSLICIASTIGYGAPCKQGSCDAHGSPLGADELQK
                     AKEALCWLADSPFSVPENVYTFFRKAVSRGKKDEKKWQESFNNFRQNEPALAAGFDRI
                     MKGDLSDGWESALSEFPNGSKDVATRKAGEIVMQSIAGKIPELAGGSADLNPSCFTWL
                     KGMGDFQSPKLSKEGLHGMVGGAWDYSGRNIHFGVREHAMGSIAVGMALHGGIIPYTA
                     TFLTFADYMRPPMRLAAMMGLRAVFVFTHDSIGVGEDGPTHQPVEQIMNLRQVPNMTV
                     IRPADANETLEAWKIAITNIKGPTTLIFSRQNLPVLDRSVYASASGTKQGGYVLWESA
                     SDPDVILIATGSEVSLTLTAARKLAEAGTKTRVVSMPSWELFDRQPRDYRDKVLPPQI
                     SARIAVEAGIKLGWEHYVGLNGKIIGMETFGASAPGPVLYEKFGFTVEKIIEAARDLL
                     HRS"
     gene            42005..43216
                     /locus_tag="PLR13_04320"
     CDS             42005..43216
                     /locus_tag="PLR13_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQC18591.1"
                     /translation="MTISAFEPATLAGIKLKNRIIRSATYEGMADEKGFPTEKLKKLY
                     INLAKGDVGAIITGYAATQADGKVSNAMAMVDHDDKINVFKEITDAVHEYGTPVIMQI
                     AHCGRQTRSRITGFPTVAPSPVRNMYFMEDKPKALSEEGINEIIDNFVAAIVRAKRAG
                     FDGVQLHLAHGYLLSDFLSSHANRRKDRWGGSTENKYRIVGEIFKRARGKVGDYPILV
                     KMNAYDGRRKGMRIEEAVVIARMLENSGCAAIEVSCGCVEDGLFTIRGEKLPVEAVVE
                     YNFNFKNYPDFAKKAIVWFAKTFLKTPKPISKYNLDAAIQIKKAVNIPVIVVGGINNV
                     ADVEDIIGNDKADFVSMSRPFIIEPDIVSKFKNRTQTTSKCIMCNFCVITIEKEPLKC
                     RYGKLPKTKSA"
     gene            complement(43248..>43721)
                     /locus_tag="PLR13_04325"
     CDS             complement(43248..>43721)
                     /locus_tag="PLR13_04325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18592.1"
                     /translation="YLDFETDERFDLILMIMCDFCALSPAQRKTMLAKFHRLLEPAGS
                     VLLDVYSLNAFNEREEKALYEPNLLDGFWSPDKYYGFLNTFKYEKEKVVLDQYTIIEA
                     ERTRTIYNWLQYFSPEDVEREFSACGFAIENYYADVAGTPFSAQSREFAIIARKL"
BASE COUNT        11134 a        10985 c        10150 g        11252 t
ORIGIN      
        1 ttggttcctg ccaactactc taacatattt tgtccaactt gaggggtgca gtccactccg
       61 cttacagcag aattaggata aactttcaat ttttgctgca catccgccat tgacagtata
      121 cctctccata tgaaataaag cactctatta attcttccgg agaaaagagt tgcaaagcat
      181 aaggcaagat attcggcaaa tcaggcagaa tcttgattcg gttatgattg ccgattttta
      241 taatattgtc cgccagctta aaaacatcga agcgcatgga gccagggatg aaaaatcagc
      301 tttcagaaag ttaggctatc tgaaaagaat tgccgatctt tccaccactc gctaccgcgg
      361 tagaaaacaa aattctcccg cggttcaatt tccgcaaaat ctgccgatca tcacgaagaa
      421 aagagagatt atcgaaagca ttcaaaaaca tcaggtcacg atcattaccg gcgaaacggg
      481 ttcgggaaaa acaacgcaga tccccaaaat gtgtctggcc gctggcttgg gcctgcgggg
      541 catgatcggc cttacccagc ccaggcgcct ggccgcggtc agtatcgccc agcgcattgc
      601 ctcggaactg ggcgaggaat gcggtcaatc cgttgcctac aaaatccgct ttgaagaaaa
      661 aagctccgcc gaacctttca ttaaagtcat gacggacggc attctgcttg cggaaacggt
      721 caggaacaga gacttgcttg cttacgatac catcatcgtc gatgaagctc atgagcgcag
      781 cctgaacatc gattttattc tgggttactt gcgcacactg ctttccaggc gaaaagattt
      841 aaaggtcatc attacttcag ccaccattga tacggaaaaa ttttccgagg ctttcaataa
      901 ggcgccggtt atcaaggttt ccggccggat gtacccggtg gaggttcgct atcggcctgt
      961 cgatcctgac aaggaagaaa aaggcgagac aacctatatc gatgaagcgg tggcctgtgt
     1021 ggaacagttg cagagcgaac ggcgttacga agatattctg attttcatgc cgactgagca
     1081 ggacattcgg gaaacctgcg ggctgttgac caaaagatgt tcagggttgg tgcttcccct
     1141 gtacgcgcgg ctatccggtt ctcaacaaag gcttatcttt gccgctgccg agcaacagaa
     1201 aatcattgtc gctaccaata tcgcggaaac atcgctgacg attcccggca ttaaatatgt
     1261 gattgacgcg ggactggcgc gtatcctgga atacaatccg cgcagccaaa ccaccggtct
     1321 gccgatcaaa ccaatctccg ccagcagcgc cgagcagcga aaggggcgtt gcggccgtgt
     1381 gcaaaacggt atttgcatcc ggctttattc gcaggaagag ttttctcaaa agcccgttta
     1441 cacatcgccg gaaatcatgc gttccaattt ggccggtgtt attctgcgga tgctggaact
     1501 taatctcggc gcagttacgt ccttcccttt tatcgacaaa cctcaaccca aaagcatccg
     1561 tgacggaatc gatatcctgc gtgatctggg agcgctgacc agagacaaca gcgctgaaga
     1621 aacaaaccaa tacatgctga ctgaaaccgg ctggaagatg gccgagtttc cgctggatcc
     1681 ccgcgcgtct cgaatgctga ttgaagcgca gaaagaaaat tgcatcaacg agatcgccgt
     1741 cattgccgcc gccttgagca ttcaggaccc gcgtgaacgt ccttacgaaa aagccgatga
     1801 ggcatccaaa gcgcatacgc agttcgcaca tccggagtcg gactttttaa cctatctcaa
     1861 tatctggaat cgttatcatg gttctctgga gagcttgacg tcgcagagca aacaaagaaa
     1921 gttttgccgc gatcattttc tctcctacaa gcggatgatc gaatggcgcg acatttacca
     1981 tcaaattctg gacatcattg acgggaaaag caaaacaaaa agcagaaaac atcacaggat
     2041 tgaaatcaat caggacattg ccgaccggat tcatcgctgt gttttaagcg gctatctttc
     2101 caacattgcc cggaagaagg agaaaaactt ttacaacgcg gccaagagca gggaggtgat
     2161 gatttacccc ggctccggtt tgttcaaccg cgccggacag tggattgtag cgggggaaat
     2221 cagcatgacc tcacgggtct ttgcccgtaa tgttggcaac atcaagagcg agtggctgga
     2281 aaaactgggc ggtgacaact gccgctacac gtatgccgcc gctcactggg agaaaaaccg
     2341 cgggcaggtt gtcgctctgg agaaggtaac ccttttcggc ctgaccattg tggagagccg
     2401 tcccgttgcg tatgaaaaaa tcaatcccga agaagccaaa tcgatattca tccgggaagc
     2461 gcttgttacc ggagaggttg cccgccggat tccttttctg gaacacaatc tgtcattata
     2521 cgagcgtgtc cagacaatgg aagagaaact gcgccggcgc ggcattgtcg ccgatgaaga
     2581 aaccattgcc aatctttacg aacagaggct gccggtgctt tccgatatcc ggtctctgca
     2641 gaatctgatt aaagagaaag acagtgacga ctttctgcgc ttccgggaag aggacctgat
     2701 tgcccgtgaa ccggatgctg aagaaatatc gcaatatccc gatcggataa aaatcggcga
     2761 tgccgctttc aactgccgct ataattttga accgggaaaa aatgatgacg gcgtcaccgt
     2821 taaagtgcct ctgggtttta tttccaaagc cgcgacagca aacatcgatc gccatctgcc
     2881 ttcgttatta aaggaaaagg ccgttcactt attgaagtcg cttcctaaat ccattcgcct
     2941 caaattgccg ccgcccgcta aaattgttga gcttttggtg gaagataaat cgaatttgga
     3001 caagcctttg cctcaggcgc tcagccagtt gcttctcgac aaatacaaga taaaggttcc
     3061 taaggatgat tgggcaacgg aaaaactgcc ggattatctg aatgtccgct acagcgtcat
     3121 tgatgaaaaa ggaaacgaaa ttaaagaaag ccgggacatt catctgctgc aaaaagaact
     3181 gaccggtacg gtcagtacat cggccatgga taaaatcaga ggtgagtggg aaaaagaggg
     3241 aatcactggc tgggatttcg gggacttgcc ccagcagatt ccccttcggg gaactcacgg
     3301 cttgatcggt tacgcctatc cggcgctgac gattagcgat gattccattg atctgcggct
     3361 tttcgccgac gaaaaacaaa gcgccttcca tcatttgcac ggtattgcgg cgctctacga
     3421 cattcacttc gccgacaaga tgaaacaact taaaaagaat gtcgctttga ctggcgcaat
     3481 gaagaccagc gctaccggca tcggccctcc caaacaactg gaacaatcca tcattaaccg
     3541 ggtcaggaaa gatttatttt acaagccgtg gcgaagacag gaggaattta tcgcgcacgc
     3601 cggttgtctg aattcccaaa ttctgcctta tggccagcag gtattgatag cgatcgaacc
     3661 ggtattgaaa gcctgtgccg atacctgtac cgttatcggc aaactcatga cgaaaaatag
     3721 aaacaacaat cctgttttga attttataaa agacatccaa acagagatct cccgtcttgt
     3781 gcccgtcgat ttccccgagc tttatacctt tgaacggatg aaggaactgc cgcgctactg
     3841 caaggcgctt gctctgcgag ccgaacgggg aaatttaaat ctttccgcgg cgcaaaggaa
     3901 aatgcaggac gttttaattt attcgcaaaa actgcagcag atggtaaatg gccgtgtcca
     3961 gccaagtctt tttgacttgg ctgtcgttaa aaaacagtcg agtccaaaag acccgactga
     4021 gcctaaagga accggattta aagaaattat tcttttaaaa gaaaacatta ccatagattg
     4081 tccggatgaa aagaaaacaa tggttgaaga attcttctgg atgatagaag aatacaaggt
     4141 ctctctgttc gctcaggagc ttaaaacccc ctaccccgtc tcgccgaaga aattgaatca
     4201 gttgatggaa gacattgaaa aatattagtg atttatttaa tccttgttca gccaagtctt
     4261 tttgacttgg ctgtcgttaa aaaacagtcg agtccaaaag accnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnncagccaa gtctttttga cttggctgtc gttaaaaaac
     4441 agtcgagtcc aaaagacccg actgagctta tgcgccttag ttcgatttta tcattaattt
     4501 tttactgcca gataaactgc ggtaagaatg ccttctccta gcagaacata ttgtcataat
     4561 cttagccacc gattctctaa tggcaaagca atgacgattc gagacactgc caaaaaaacg
     4621 tttctagggt tgcggaagtc accaggcata aaccagcacc tcatgcaccc gtgcctaatc
     4681 gatggcatga ttcaccgtca gcaataatga atttcgaccg ctaagtagtt ttcatcaatt
     4741 ccaggtattc cctggtgacc tcatacaccg cgtaatcaaa cagcaggata gttcggttga
     4801 gggtatccag ggattcctgc ttggcaatag ctgagttgaa gatcatttga aattccgcgt
     4861 atagccagat attccgtcgc atcaatatca gcgcgtaaat agcttctttc gcgggaatgt
     4921 caagagcata acattccagt gcgtatttct gaaaatactt cgtcacgtca tccgtaattt
     4981 tttcataaat gaacatctta ctgaattgct gataaaactc tgtacactga tcgactaatt
     5041 gcttttcatt cagcttttta taagtctgcg ttcgggtgtt gtttctaacg tcctttaccc
     5101 atcttgcggc aatcttctcc gcgtgttttt cagccagttc cagatattta taggcgtatg
     5161 ttttcatgtt tgatttcttt ttcttgtccc gggcttgtct ttctgccagc tccaaatatt
     5221 tcaaaacatc tgatttcgat ttcatagttc atctcctttt attagcggac tgaaggaatt
     5281 gatttagcgt tgttggcgct gtcgaataag ttttttcacg aatatttcct cgcgttccat
     5341 ttcctcaatc ctgtcggtga tgtatttttt agcctcctca tgttgtggaa taaagatctt
     5401 ctccagagcg ttcacccgcc gttgcacttt tttcagttct cttgaaagca tgatgacgga
     5461 tttcattatt gtcgcgtatt gcaccacgat gtgaagagct ttccgagatt gttcttttgc
     5521 gttatcataa gcagatgttg ttggcggcaa agtaaaagcg ggaagattgt tcttgagatc
     5581 aagagtgaaa tttaacagtt tcagtcccat cagttttgtt aaattcatat gaagataata
     5641 gcttctcatt tcggaacaac tttgtaatgt aacagcttcg gaacccatat cgacggcagc
     5701 ctgtcgataa gccaaatata aacgatttac aatatcctga aattcggctt caattttccg
     5761 gatgacatca atcttccgaa cgatttcaat agccagaatt tcccgtttct gattaagcag
     5821 atcatagcct tcgtaagcga aagccagatc ttccttgagt tttcgcagcg atgattttgt
     5881 cgggggtaat tctattttcg gcattacgtt ttctccgtct cagacatata gtatcgctgg
     5941 atatcctctc tagcaatgcg atacaattca tcacggggaa gcagagaaag catcttccaa
     6001 ccaatttcca gggtctgttc aatggaacga ttctcatgat aagcctgacg caaaaaagcc
     6061 tcctcaaaag cggttccgaa tttcaagtaa attttgtcca gatcggaaag ttcctcctct
     6121 cccacgatgg ccgccaacgc tcttatgcgt ttgactttag catacgaggc aaagagctgc
     6181 gcggcaacct ggccatggtc cttgcgtgtt cgaccttcac cgataccgtc tttcatcagc
     6241 cgggaaaggg aagggaggcc ggcaatcggc ggatagattc ctttattaaa cagctctctt
     6301 tccagcacaa tctgaccttc ggtgatatac cctgtcagat ccggaacagg atgggaaatg
     6361 tcgtcgctgg gcatggtcag aatcggcatc tgggtaatgg agcctttacc gcctttgagt
     6421 cgaccggcac gttcatagac tgaagccaaa tcgctgtaaa gatagccggg ataacctttt
     6481 cgtgacggaa tctcaccacg cgaactggag agttccctca aggcttcgca gtagtttgtc
     6541 atatccgtta aaatcaccag tacatgcatc ccttcttcga aggccaaata ctcagcaagc
     6601 gttagcgctg aacgaggcgt tacgagccgt tcgatggatg gtgagtcggc aagactcagg
     6661 aatattgcgg aatgcgtgag aactcctgac tcttcgaggg aatcgatgaa gaatctggct
     6721 acgtcaaatt tgactcccat cgcagcaaag ataacggtga actcctcttc tgcagtcaga
     6781 attcttgcct gcctggctat ctgggcggca atacgattgt gcggcatacc gcttcccgag
     6841 aaaatcggga gtttctggcc gcggatcagc gtgttcatgc cgtcaatcgc cgatatcccg
     6901 gtttgtatga aatctctggg atattctcta ctccagggat taatcggcag accgttgaca
     6961 ttcatcatcc tgtctgtgat gacctgcgga gcgccatcaa caggtctgcc caggccgtca
     7021 aaaactctgc ccagcatttc acgatcaaca ggtatttcca acgcccttcc cacaaaggtg
     7081 actcttgttt ctttcaccga aaggccgctt gtcccctcga agacctgaac aacggctctc
     7141 tgttcatcca cctccagcgt ctgaccgata cgtaaatctc ctgaagcatc gcggatttct
     7201 acaatgtcgc cgtatccgac gcctttgatt ccgtcgacga aaatcagggg cccggaaatt
     7261 ccggaaaggt ttgtatattc aatttccatg gtcattttac aatcctcacg atgacgattt
     7321 tatcgacatc aattcctgtt ccagtctttt caagtaatcg tcaatctcaa caagttcttt
     7381 attgggaact gccgacttaa gccgtaccat ttccgaaaca acgtccgatt gactgatatc
     7441 tttgacgggc acaccttctt gaatcagcgt catgcctttt tggtagagga ccaaaaggca
     7501 ctttaaaagt ttgagttgct tttccggact gcagaacttg tcctgcgaat cgaaagaatt
     7561 ctgctgcagg aaggcattct tgatcatttc ggcgcagaag agaatatact gttcagaagg
     7621 cggcaatgcg tcactgccga caagcttcac gacctgctcc aaacgatggt cttccaaaag
     7681 aatattcatg acctcatttc tggttgtcag ccattcgctg tcgagatttt tccaccaatc
     7741 ttcaatatcg acaaggtatt ccgtataact gtcgatccag ctaatcgacg ggtaatgcct
     7801 ggcgtcggct aaaattttat caagcgccca gaagcatctc acaaatcttg atgtgtgttg
     7861 cgtgaccggt tccgaaaaat ctccgcccgg aggagaaacc gctccgatga tggtaatgtc
     7921 gccgttactg ccgctcagcg tttccacaag accggccctt tcgtaaaatt cggccaaccg
     7981 tgtacccaaa taaggcggga atccttcttc ggccggcata tcgcccagcc tgctggaaag
     8041 ttcccgcagc gcttccgccc agcgtgatgt ggaatcggcc atgactgcca cgctgtagcc
     8101 catatcgcgg tagtactccg caatcgtcac gccggtatag atgcttactt cgcgggcggg
     8161 aaccggcata ttggatgtgt tggcaatcat cacggttcgt tcgatcagag gccggccgtt
     8221 gcgttcatca ataagtttcg ggaagtctgt gagaacatct gtcatctcat tgccgcgttc
     8281 gccacagccg atataaacga taatatcggc gttacaccat tttgccagag catgctgtgt
     8341 catggttttt cccgtgccaa agccgccggg aatggctgcc gttcctcctt tggcaatggg
     8401 gaaaaacgta tcgataattc tctggcctgt aattaacggc ataaaaggtt tttttctgtt
     8461 tcggctggga cggggcttgc gcaccggcca ataagcggca agtttcccgt tgtatttgcc
     8521 gtaacctgta tcgatttcaa agatatcgtc ttctatcgtg taaggcccgg cattcacgat
     8581 agcagtcaat ctccctgcaa tgcccggcgg aacaagcacc cgatgtacaa caagcgggct
     8641 ttctttaatc tctccaacca gagtcccctc gtttatatcc tcaccggttt tgaccagtgg
     8701 ctggaagtcc cattttttat tgacatcgag gggcggaagt ttaatgcctt ttttaatata
     8761 gcttccgcat acctcggaaa taccgacgag aggccgttga ataccatcgt aaatattggc
     8821 tatcagtccc ggccccagga gcacagaaag aggacgtccg tatgaaatga cgtcgtcacc
     8881 cggcttcaag cctgtgttat cctcatacac ctggactatc gtgatttctc cggcaagcct
     8941 gatgacttct ccgatcagtc gttcctgtcc cacttcggca atgtcgaaca tttttattcc
     9001 ggtcagcccg acaaccttga caataggccc attcaccgaa atcacatttc cttttaatat
     9061 ttcgtccaca tacgctcctt aaaagttaac tgcgtcattg atttccttcc gctttccgca
     9121 tcagtaaact catgacttgc tgcctgatca gcagagattt tctgaaatag attctttcca
     9181 gcgtatgatt aaaaatacga cctgtttcct tatcccggat gagacatccg ccccattgga
     9241 ctttcgaatc ttccgtgatg gaaactttct ttcttccgtc catagccgcg atggctgcga
     9301 cgatatcttt cttcaggaaa atatcctcgt tgctgagttt tatatctaca tcagcacccg
     9361 atatttttac gccatcgcgg attgcttcca gaagaaattg tttgtaaagg cgatcgttac
     9421 gaatgttttt catgacctca tcgacaatgc gattgataaa ttgctctgca ttctgaagct
     9481 tcatcttttt tcgctcgaga agcgctttat tttccagttt agaaatttcc tgccggatgt
     9541 gactttctgt ttccgcttca gcctgcctcc ggaagtcctc catctccatc gaaaagacct
     9601 catccctctg cctcatctcc agagcctctt tttccctgat ggcggttatt gcgcgagagg
     9661 cttcttcacg gatagaattt tccaatgttg tattgtccat gctctatcct tacaaacgaa
     9721 taccgagagc ctcggaaata taattcattg ccgacatacg cagccccccg atatcatcca
     9781 tgcccggaat ttctatcacc acagggttct gcctattgag gttaatctct ctgatacgct
     9841 cctgtaatgc tgcagcaagg tcattcgtca tcaagacgat accggcatct ctttttttta
     9901 taatttcttc gagtcgcacc gatgcactgt ctgccgttga gaccatccct tccaccccgg
     9961 caaggcgaaa cgcgctgacc gtatgcatgt ctccgatgat gtatatcttt tccatgacat
    10021 tccctttcca agtcaagcgc tacctttgtt ggcgtcgtcg gcttcctcga cgtataacct
    10081 aaaggtcact gggttagcaa tacgcctgcg gagcctcctc cttgccccgc tgaagcggga
    10141 cagttcaacc atttaatttc ggttcacttc gtttccgtaa tttaagtttc actgccgccg
    10201 cggtatcatc ctgatttgaa aatgtaatta cagtttattt aaaatcatga tcgcaataat
    10261 caaaccgtaa atcgcaatgc cttccgccag cccgagaaaa acgagcgttc ttcccatgag
    10321 ttccggcttt tcactcactg cgccaatggc ggcagaccct atccgtgcta cggcaaagcc
    10381 gctggcgata caggccaacc ccacagccag ccccgcaccg atgtaagcca aacccatcca
    10441 gtttgctccg gagttaacct gaacattctc ggctgcatta atgacggtgg gcaacaatgc
    10501 gaaccaaatg aaaccggtga agactgcaaa cacaaaatgc ttgaacaatt tattcttgat
    10561 catcttacac ctccgatgct tgtgccttta aggtaaatgg agaaaagacg actccatctc
    10621 cctggaaaaa tttgctgaag aattcataat attcaagacg catggattga atgccgcaaa
    10681 ttaaaccttc aaaaccgatg ataaaaatat taccggtaat gatgattaag atcgcacccg
    10741 ctgattgcaa accggggtcg gcaattccgg caagcgtgta ggtcacgata ctcaaccccg
    10801 catgtgaaag agcaaaagca ccgactctga taaatgatat cgtgttggca aacagactca
    10861 gcccggtttc cacaatttcc atgacggttt ctatgatata ttctgaagca ctgtgcgggc
    10921 tctcttgatg aaaaaatgcc gggccaatga cgcctctgag tgcaaacaat gtcaggggca
    10981 gcattataaa agcgctgatc tcccatattg caggaacctg accggacctt tgataattga
    11041 acgcaaaaag gacagtagcg gcataaatta ccagaatcac cagtccccgc ttatccagaa
    11101 gggcttccgt gtaatccgcg ttcaggatat tattgatgat gttcacaaga agaccggtca
    11161 tgataaacaa aatgcctatg agaatcgttg cggcaaacag tgtcataatg tttgcagtcg
    11221 ggtgaaacca aagggctgga agcaggtgtt cgctggaaaa aacgctgcca taaattaaac
    11281 cacaaatggc ggcggaaaat ccacaagcga ccagaatgcc gccggcttgt ttcagatttt
    11341 cctttatttt tttttggcca tactttttca gcattatccc gcaaagcagg agcaccaatc
    11401 cctgacccag atcaccaaac atcagaccaa acatgagcac aaaagtgatt gcggtaagcg
    11461 gtgtcggatc aatttctcta ttagacggaa tacccattgt tttcacgatc agctcgaacg
    11521 gcttaaaaag tttcatgttc cgcagatgaa caggggcatc cggatcttta cgctcagaga
    11581 aaataaatct attgccgcaa atcttttcca gaattttcaa aagccgctgc ttgtttcgga
    11641 tgtctaccca acccgtaata aacaatgcct tcgctgaaaa tagcgacatc cgcatcgcct
    11701 tgagtatttc ctcgtagtga tggtaggatt tattcagatt ctgaagccag cggccttgtt
    11761 ctgtcttcag tcgatcaaga tatgcatcca tcatgtcgcg gcgccgtttc agaccttccc
    11821 gccgcttttt taaactttcc aaagaaacag gagttacatc atctgataaa tctataaatc
    11881 catagttttt taaaaaacga aagagagccg gataatcatc aggcaagcaa ataccgagaa
    11941 cgtaattgtt aattttgttt aacatgaaac gactgttcgg tggaacatca tccgtcatat
    12001 tttccaccgt gccgaaaagg attttaacaa gcctcgcctt ttttatcgcg acaggatcga
    12061 tagccatctt actcaatgct tcagcgtacc ccatctgtcg aatgataatg ttttcctttt
    12121 ccctgatctt tgccctgagt cgcacggctt gttcaatggt tttttttatc cgggaaacaa
    12181 aaatcaaatc gccgctctta tcgacagccg gcataaatat ctcggattct tccggggcaa
    12241 tcatcagtgc attcaacgcg tattctgtgt tcgaaataat ttctctggtt aatgcctctt
    12301 ctttctgaag tccgctgtcc gttccggaat ccttctctga aaaacgcgcc agatgaatga
    12361 tacctgcctg acccaatgca aggtatacgt ccgaataata ggctttttcc aaagcgatcg
    12421 taatgtgacg tatgtcagat tttaaaaaca tttttttaat tattctttac atattatttt
    12481 actgagtatc gcatcggctg aaaaaccgaa acgccggccg tcgataatac gaaacaggtt
    12541 ttttatctga atgaggagta accctagata agcaataaca ccgtaaatcg agtgaaagtc
    12601 tcgataaaaa attgatgaaa gccataggta ataaatctgc tcaagatgct gttcgatatc
    12661 aatgacagat ggttcctgcc cgctgctttt gcgcaacgtt tcaatatgct gatttaaatc
    12721 ctcgtctatt gaatggattt gagattccgc atggatgttt aagcgattgt ttattttttc
    12781 caaataaagc cgggtgtcct tctcgctcca gtgatagtaa acgcgaagcc tgtatgaccg
    12841 gatcaccatt tgtaccgcaa ttcttctcag gatcatttcc tgaaattctt ttctggcctg
    12901 gtcggaaatt aaagatgaag cgttatagat atttctcgcg gaactgatat ctaaattcat
    12961 ctgcattcgc cggtaactgg aagagggctt gaaatcggct ccaagataag tattggtaag
    13021 aagtgacgtg acatcatcca tggatagatt cttgttcaac agacttttat ccagggaagc
    13081 aaaaggactg atatcatacc attgttccag aatgtccttt cctgctgcct ttgccagaag
    13141 aaccttggcg ttgtgtgtct cgaattgacg gagataagca agaaatactg gagtgtattt
    13201 gtcgtatgct tcaaccagtg cgataacagg tgctatctgt tccctaaaaa gattctcttt
    13261 tgcatcaatc agatcctgaa cgcttgatga ctttggtgca aacgtccgtt gttccctggc
    13321 catgacggca tagtcctgca gagagaggag ccgccctttc atggcatata tacgggcgtg
    13381 aagattagcc tgatcggcgt aatttctcat gaccactagc ctccaagtaa aatggaaact
    13441 atgtcttttt ttatcgcctc gttcagcccc tgatctcgga aaagattttc catatccgta
    13501 ataaaatctg cagagacggc ctctatgttt cgttttgcgt cttctacggc ttgagactgt
    13561 cttttgtttt ctgcggataa aatctcggcg cgttctcggg tcttctttgc ttcaagaaac
    13621 cgtttgtggg cttcaatgtt tttcccgtac tcaagctgtg ttgcttctac atcagcagag
    13681 cattgctttt caatatcgac aatccgggtg attacatcat ccatatcggc cacccatgtc
    13741 ttgatatttt ggttgttaaa agtttttacg tacaggttga aaacatttga agacaaaact
    13801 cctgtgatgg agcatgccct tatgcttgct tgatagtaaa tgatttttac ttatttatat
    13861 tataggtatt acgatatggt atgtcaatga gatattgtct ataagcgtga aaacagactt
    13921 ttatcaatat tttgaacagg cacgccaaag ctggtgaatt tgatgttgaa gccgtgcttt
    13981 gcagcaagcg ctttgatccg ggcaacctta tgcaaatccg gtctgaagcc tgtggaaaaa
    14041 tcatcctgcg tctgtccttc aaaagcgagg atcatggttt ccgccatgca ggcgtaaata
    14101 tatccgtctt tgatcggcag ttttaatccg agctgcgcgg tcggcggggg ttcgatcact
    14161 gcgccgtcaa taacaaggat gtctttacgc tgatcgcaga tttcccgggc aatgttacgc
    14221 ggggagggaa tgtcacagat aatcgctcct tttttaagat tcttagctgt tgctgcggaa
    14281 ggcacctcga tggcggacgt ggtgaagatg acaagatcgg cttccgtgat gacatcctcc
    14341 aacgcaggac cttgatcaat aatatttgtt tttgattttt gagcgttgag aaattccttc
    14401 agctccgcga gccggtcggc gttcctggct agcagaaaaa ccgtatccac cctgttgaga
    14461 aatcccagag cgcaattctg gccgatggcg ttggttgcgc cgacgatggc tacttttgcc
    14521 ttggagagat ccacattacg catcgccgcc gctctgtcca gcgtctccag aatggctgcc
    14581 gaggccaggc tcgaacccgt cgtgatcgcc gcgggcgttt gctccgcaat cctctgccct
    14641 ccttttccgg caacacccat caaggctccc agcccgacaa cctttgctcc gcttcgttcc
    14701 ccgatccgca ccgctttcag gatcttattt aaaacggcat tttccttcat agcaagaatc
    14761 tgttcagaaa gcagcggaca gacaatgaag ctgccatcaa tttccgcacc agcggcagat
    14821 tgcaaatttt ttgcacgatg aattgtaaaa ggcggcaaca accttacaat tatttttatc
    14881 aacgcctgag gtgcaagtct tgaaaggaaa caataatcgt aaacattttg aatattgaag
    14941 ggattgagta caaatgcaaa tttgatcata taaaccgatt cagcgatatt ttatttactg
    15001 atttggattt catcattgat tttaatttct ccgccgatga gcactttagc gaatatacct
    15061 tcccggggca tgacgcaatc gcccacagta taaaaaatat tgcatcgatt atggcagacc
    15121 ttgccgattt gcgaaacctc cagcagaaca ttggcgccgg cttgcaacct tgtaccgaca
    15181 ggaagcgacg gcagatcaat tccttcggtc gtcagatttt cggcaaaatc gccgtattga
    15241 acgtcaaggc ctttggctct gattttttca atgctttctt tggccagaag actgacctgc
    15301 cggatgatat cggcctgggc atgggcatcg ctttccagcc ccagattttc taaaaaaaga
    15361 ccgcagccga tattattctt ttttccccct tttttctgag agatgttcac cgccagaatt
    15421 ttcatttttg tttcttttac ctgtattttg ccttatccga aagggatgac tttctcaatg
    15481 atcccgccgc ccagaacgca atcgtgatcg taaaagacta ccgattgacc cggtgtaatt
    15541 gcctcctgtt cttcggcaaa gataactttc aatttttctt tttcttcccg gacttcgcaa
    15601 agcgcttctt ttttccggta tctgatcttc gcatgcatct gctgcggcca ggatttcgcc
    15661 agcctattga tgccggacgc gataagcgct ttgcccatca agtctttctt atcaccgaca
    15721 acgatgcggt tgttcccggg atgaatggac aatacataca acggcgtggg atggctgata
    15781 cccagaccgc cccgctggcc gatagtatga aagataatcc cgcgatgcct gcccagcaca
    15841 ttgccccgca catcaacaat cggacccggc gcgatgtctt ttactctttc caaaagaaat
    15901 gtctgagaat ttttctgagt cacaaaacaa atatcctgac tctcttgctt tgccgtcacg
    15961 ggcagagaat ttttcatcgc caaatttctc acttcatcct tcgtgagttg agcaaggggg
    16021 aataatatat tttttaatct ctcataggga atggcataga gaaaatacgt ctgatctttt
    16081 tttttatctt tcggccttgt aagacaataa ccgccttcgt ttttttcaat ggccgcgtaa
    16141 tggccggtcg ccagataatt gaaacccagc tcctgcgcct tgtccagcag tgaaccgaat
    16201 ttcagatggc ggttgcagcg aacacaggga ttaggcgttc tccctttctg atattcgctg
    16261 ataaaatcgg caatgacctc atttcccagc cgggaggcgt aatcaaaaac ataatgggga
    16321 atgttaagac aggcgcaaac tttttttgcg tcgtcaatcg cggcggacgt gcaggatctt
    16381 ttcccgtctc cctcggtcaa ggcgccaaaa tgcatggtca cgccggcaac attgtaacct
    16441 tcattaagca gcatccatgc ggccaccgat gaatcaatcc cgccgctcat ggccaccaaa
    16501 actttatcac ccattcttat cctcaggatc aaatatcaaa ttcatgtttt gcagcagtta
    16561 tcttttcttt caaatattct gcataattat ttttcttttt tctgattcat cttcgcaagc
    16621 cgatcaaggg ttatggagct caaggtttct gaaatttttt ctccaacaac cgcccagaca
    16681 tcatggctgg cacatgtcgt cacgcgggga caggcggccg gattattaac gcaatcaacc
    16741 agaaagcggt ctccttccaa aacatcgaca acctccttta aagttatctc cgagggatct
    16801 ttgttcaggt tatatccgcc gtaagctcct cggaccgaat tgaccagacc accccctcgc
    16861 aaagggataa taatcagact caggtatttt tcggatatgt tttcttcttt ggcaatattt
    16921 ttgaggaaaa cggggtcttt gccgtaattg cgggccaggg ccgtcatcag tcggacgccg
    16981 tagcgcgttc gtgttgaaat tttcattacg ctgtcctttt acatcatact tactgatact
    17041 atcattatac tagtaattaa agtaacagat atgtcgtgtc aagagaaaat gattgtttta
    17101 tgaaacggca atatcgtcgg cctcttcgat ttatctttct aaaaacttaa ttcatgaaga
    17161 ttatacccgc acctcccatg cattcaatat acgcaaaaac atttcgtacc tgatcaacga
    17221 attaatattt ttacttgcaa cctcatacct tgagtattaa atttacaaaa aaatttatgg
    17281 agggcgctta atatgaatat taaggggagt tcctttgtca cgaccaaggc tatcataaca
    17341 gaagcgttcg gcgaagagcg atggaatgca tttcacgccg gtcttaccca aaaagatggt
    17401 tttttcaaca atatgattat gtccgttacc cttattccgg tagagaagat gattgttttt
    17461 tttaatgaac tatgcaggga atttttcaac aatgataaaa ctcaatacgc catgtttgga
    17521 aaatcgggcg cgaaatttgt tctctctccg acaggtccct acaaatctta tatgcttgcc
    17581 aaggatgcca ggcagtttgt cgaactgaac atgccaaaaa tctggtcaac ttattttgat
    17641 ggcggtacgt ttacgtcaaa tttcgataac aacaccgctc atatcactat caccggaatt
    17701 ccagtgaaga ataactattt cgaggagctc gttatgggat tcaaccagca ggcgttcaaa
    17761 atattcggtg aaaaaacaac cgcggtcaaa gtcagtagca tcgcagcagg tgatgatgat
    17821 tttcggtttc agctaaaact gaaggattca tgaccacaaa cataaatggt tgctttcgcg
    17881 ggattgcgag aaaagctttc tatattaaac caggcatgaa tttgtggcta aaccattcat
    17941 tagtcttccg ggatcgggtg gtccggattt ttttgttatc gatgaaaagg ataatgaaaa
    18001 aataaggatg ttttatctgc accgatactt caatgaatcg gcactttatt tttttctcag
    18061 gtttcctaat cagcagaaaa tcaaatctct ctgtttattt ttttcttctt tcgtcaatat
    18121 cacccggata tatcatcgca ccctctccgc gctcacgcgt gcggacgcga atgacatcct
    18181 ctaccggtgt gacaaaaatg attccgtcgc ccgtctcgcc ggtgtaggcc gctttaagaa
    18241 tcgtctccag agccgcttcc aggttgcgtt cgcttaaaac gatattgagt tgtatcttgg
    18301 gaagcagatt gacctgatag gtaccggcgc gtccgaccag ctggacgccg ccctggcggc
    18361 cgtgtcccct cacttcaaac atactcatac ccacaatgcc aatctctttc agggcgcaca
    18421 tgacatcgtt cactttatct tcgcgaataa tggcttcgat ttttttcagc atggtaacct
    18481 ctccggttta aaaataaaaa actgatcaat gtttccattc aagcatgttc tgttagtagg
    18541 accgttctcc gtgtgaactg atatccagtc ccacttcttc ttccatcgga ctgacgcgca
    18601 atccgatgct cacgtgcaga accttggcca gaatataggt catgccgaac gaaaagagta
    18661 tggtgaccaa aacggcggta atctgaatgg cgagttggcc ggcattcccg aaaaacatgc
    18721 cgttggcgcc gtcggcatta acggccacgg tagcaaataa tcctgtagcg acggtgcccc
    18781 aggtgctgcc catgccgtga caggcccaga catccagaga ctcgtccagt ttgcgccgtt
    18841 cccggaaacg gatggcataa taagcaagag gagcagacac ggctccgatc accatcgcgg
    18901 ccagaggcgt tacaaaaccg ctggccggcg tcacggctgc gagtcctaca accatgccgg
    18961 tggcgatgcc cagcgtgctc ggtcttccgt ccagccagga aagcagcatc cagaccagcc
    19021 ccgcggccgc cgccgacgta ttggtcgtca ccagagcgtt caccgccacg ccgttggccg
    19081 cgagagcgct gcccgcatta aacccgaacc agccgaccca aagcaaaccg gttcctaaaa
    19141 ccgtaagcgg aatattgttc ggttccatcg gaacgcttcc gtgacctttg cgcggaccga
    19201 tgaccaatgc aaacgccagg gcggaaaaac cggcggcaat atgaacgact gttcctccgg
    19261 caaaatccag aacgcccatc tgtttcagcc atccgccctg cccccagacc cagtgacaaa
    19321 gaggattgta aaccaatgtt gcccagagca ggctgaacat caggaagctc ttgaatttta
    19381 ctcgctccac aaaagcccct gtaatcagcg ccggcgtaat gacggcaaac atcatctgga
    19441 aagcggcgaa aagctcatga ggaatggttc tagaataagc ggcgttcggt tcggcgccga
    19501 cgccctgaaa gccgatgtaa tccaatccgc cgatcagtcc gccgatatcc gggccgaaag
    19561 aaagagaata tccgaaaagc acccactgca cgccgatcag cgacataaag atataactta
    19621 acgttaaggt ggatagaaca ttttttcttc tgaccatgcc gccgtaaaaa aacgccaggg
    19681 cgggggtcat caagagcaca agagaacagc aaaccagtat ccatcctgta tcgcccgcgc
    19741 ttatctgtag atccataatg ttcctcccga tgaaaataat taatgattag caataatcct
    19801 gccatcacac attccctata aaatacgtaa ggttatgaat ttggcacggg aggaaatttc
    19861 tacatctttg tgctcttcgc ctaaagacag aacacaattg taacagttta aagccgacaa
    19921 cctttcgctg atcagaattt ttatacaaaa tgtaacaatt ctgtaaaaca gtttaacgtc
    19981 tttatgaaac atccatcaga ataattgaaa taaattcccg cctggacaat cacgatttca
    20041 tcattgacat gctatatcat tatccctata ctttcgaagt cttaaataaa tatgccccaa
    20101 ggagaaaacc atgaccgcac cgctcattat tgccatgtcg caggggaagg aaattacatt
    20161 actgccgcaa atggccaacc gccacggttt gataaccgga gcaaccggaa ccggtaagac
    20221 tgtaacgttg cagaaaatgg ctgaatcctt tgcgtccatc ggtgttccag ttttcatggc
    20281 cgacgtcaag ggcgatcttt ccggcatcgg cgcggccggc gttgcctcgg aaaaactgct
    20341 caaacgtctg gaaggcatcg gcgtgaaaga ttatcagcct cgggccaaca cgacggtttt
    20401 ctgggatgta tacggaaaat ccggtcatcc ggtgcgcgca accatatccg atatgggacc
    20461 attgcttctg gcgcgtctgc tgaatctcaa cgacacgcaa tccggcgttt tgcagattgt
    20521 cttcaaaatc gccgacgaca acggattgct gctgatcgat ttgaaagatc tgcgggccat
    20581 ggttcagttt gtgggagaca atgccaaaaa attcaagacg gaatacggaa atgtctccgc
    20641 cgcctccatc ggcgcgatac aacgcggact gctcaccatt gaagagcagg gcggcgacaa
    20701 attcttcggc gaaccgatgc tggacatttc ggatctgatc cagacggaca gcgagggacg
    20761 cggggtcatg aacattctgg ccgctgatca gctctacaat aattccaaac tttattcgac
    20821 gtttctgctc tggatgctgg ctgagttgtt tgagcagatg ccggaaatcg gcgatcccga
    20881 aaagcccaag ctggtcttct tttttgatga agcacatctt ttgttcagcg acgcgcccaa
    20941 ggcgcttctg gaaaaaatcg agcaggttgt ccgtctgatt cgttccaagg gtgtgggcgt
    21001 ttattttgtc acccagaatc cgctggatat tccggatacg gttctggcac aattgggcaa
    21061 ccgtgtacag cacgctctga gagccttcac gccgcgtgat cagaaagcag ttcagacagc
    21121 ggcttccacg atgcgccaga atccggcctt tgatgccgaa caggccatta ccgaactggg
    21181 tgtcggagaa gcgctgattt ccttccttga tgaaaaaggg cgaccgaaca tcgtcgaacg
    21241 atgcaatgta attgttcctt tctcacgcat gggcgccatg acgcccgatg agcgcctagc
    21301 cgctatcaag gcttccacga tttacgggca ttatgaaaaa accgtcgatc gcgaatccgc
    21361 ctatgaaaaa ctgcgggaag cacaacagga agaggaaacg cctgaagaaa aacccgccat
    21421 taaagtggag cgttccgctg ctccggcaaa aagtgaagaa gaaaaggaca gcggaggcat
    21481 gggggatgct ctcagcaaaa tgattttcgg cagcaaaggc ccccgcggag gacagagaga
    21541 aggcatggtg caggccgcgg ccaaaagcgt ggcgcgcagc atcggttctt ctttgggacg
    21601 ccagattatc cggggagtgc tgggatcaat tctgggcggt tcccgcggtc gctaatgaaa
    21661 atttcccgat tttaacggca caccgctttg gtgaagaaac aattgctttt cacaagatat
    21721 tgatttagaa ttcataatat aagtcaatag caatgtttga aaaaccgcaa aagcaattgt
    21781 ttttcatcct ttgcatttgc aatcaggcaa tgctgtaatc attagatttc acacatgtta
    21841 tgtaattttg tcacttcttg attctttacc aacgaattat ccgggaaaat tgaaggatag
    21901 gaccccttat tttccaccac cttaaaacat gacgacgcat aaaatatttt tcaagaacag
    21961 acgtgatgtt gccatcaaag gaatgttacc atgattgaaa ttgatatgag gatcatcttt
    22021 gccgaatatg gcttgtctgt attgctttgc ctgatcgtta ttgtttctct ctggtggcaa
    22081 aaccgcaaac gtgctccgga aatcattctt tggcttatcg attatattct tcaatttgcg
    22141 ggcattatgc tcatcgcatt gcgaggcatc ctgccggact ttatgacaat cgtcgtcgcc
    22201 aatctcttta ttgtcggcgg aacggtgatc ctttacgaag gtctttgccg ttacgtgggg
    22261 caggaaagtc gccagaaaca caattatgtc atgctggcca ttttcacctc ggttcacatt
    22321 tttttcacat atattgcccc tcatatgggt ttgcgcacga taaatttttc agtcgcgctt
    22381 ttttatatct gtatccaggg cgcctggctg atgctttaca gggtggcctc cccgttgcgt
    22441 caggccacaa aaataacggg aattgttttt gcggcgtttt gcattgtcaa tatcatccaa
    22501 atcatcataa atttgattaa aacaccgtcc gacaacgcct ttgtatccgg cgctgcgggc
    22561 gtcattgcgg tcctgattta cgaaacatta tttttcgcct tgacattttc tctttttctg
    22621 ctgatcagcc ggagtcttgc catagaactt gagaacgaac ttacccggcg gatgcggacc
    22681 gcagaggaat tgcgtcaaaa tcaggaaaaa ttcgccaaag cgtttcagac atcgccttat
    22741 gccatctcca ttacgcaaat gaaagacggc aaattcctgg atataaacga cgcgtttgtt
    22801 aatattacag gttactcgcg tgaagaaatc atgaaggaat catccatggg attgaatctc
    22861 tgggtggata aagaagatcg tgaagaaatg gtttccattc tccggcaagg attgccggtc
    22921 gtacgaaagg agtacaggtt ccgcaagaag aacggggaga tattgaccgg tttgtattcg
    22981 tcgcagatca tcaacattac cgaggaaccc tgtcttttat ccagcatcaa cgatatcacc
    23041 gagcaaaagc aggcggagtg gacgcttaaa gaacgaatta aagaattaaa ctgtctctat
    23101 ggtatttcgg ctgtgctgga tgagcctggc atatcattaa acgacatcat gaataaggcg
    23161 gtcaagcttc tgcctgcggc tatgcggttc cccgatataa caggggccag gattattctg
    23221 gaagaaaccg tttttcaaac aggccttttc aaagaaacgc cttggcagct cactgaaaaa
    23281 attaccgtaa acggcaaacc ggaaggtcag gttaatgtct gttatccggc agaacggttt
    23341 cagacggatg aaaaaccttt tttagattcc gagcggcaat tgcttaaagc cgtagccgaa
    23401 cagcttggcc atttcatcga gcaaaaacgc gccgaagaac aaatccaacg caacgcaacg
    23461 cgcttgaccg gcctcttaaa tatcatgcag catcgtactc agacaacaca ggagtttctt
    23521 gattacgcgc tcgatgaagt catcagactc acggagagca aggtcggtta tatttattta
    23581 tacaatgaaa acagccggca atttgtcctg aactcctggt caaaggacgt catgaaagaa
    23641 tgcaccatcc cgaatccgca gacgcgttat gaactggcca aaacgggaat ctggggagaa
    23701 gcggtacgcc agcgcaaacc gatcattgtg aatgactttc cggccgatca tcctctcaag
    23761 aaaggatatc ctgaaggcca tgcgcccctt ttcaagtttt taaccgtgcc ggttttcgat
    23821 caggatcaaa tcgtcgctgt ggtcggcatc gcaaacaagg cccgcgacta cgacgaaacc
    23881 gatgtgctgc aactcaccct gtttatggat gccgtctgga aatcggtgga ccttaaaatg
    23941 ggcgaggcga aattgcgcaa aagcgaagaa aggttcaagc aacttgccga agtgtttccc
    24001 gaaacgattt tcgaggcgga cgcaaaaggc aatctgacgt acgccaatga acacggcctc
    24061 aaacatttcg gtcttaccga acaggacatc gcccaaggtg taaacatttt tgattttgta
    24121 tcgccgaatg atcgtaaagc agcttgggag aaaatacaga acagaataca gggcacccaa
    24181 aagggatacc tggaatatga agctctgaaa aaagacggca gtccttttca tgctctggcg
    24241 ttatcggtac cgatcgcggt ggacggcgta ccggtcggta ttcgcggctt tattctggac
    24301 attaccaaac gcaagcaggc ggaagaggct ctgcgtaaaa gcgaggaaaa ttatcatttt
    24361 attgccgatc acaccgccga tcacatctgg acgatggaca tgttcatgcg ctttacttac
    24421 tccagcccct cggtaatcaa aacactgggc tatacggctg acgaattcat ggcgcgaaag
    24481 atcgacgaat ttttcacgcc cgaatcgctg gttgacgctc agaaattgct cgcagaggaa
    24541 ctcgaacgag aaaacgatcc gaccgccgat ccgaaccgca gtcgaacact tccatcccat
    24601 ctccgccata agaacggaca ttgggtctgg ctggagagtt ctctgacgtt tattcgcgat
    24661 gcgtccatga agcccatcgg tgttttaggt gtcacccggg atatcaccga acgcaaaaaa
    24721 tccgaagagc agatcaaaca tctggcaacg cacgatctat tgacggatct gcccagttta
    24781 cgattggcca gagatcgcat ggtctcggca atgaacatgg cgcgccggta caaaaagtca
    24841 gcggcgatca tgttcctcga cctggatggt tttaagaacg tcaatgacac gctcggccat
    24901 gatgccggag attacgtgct caagcaggtt gctcagcgtc tgctttcctg cgtgcgcgaa
    24961 accgatacag tcgcccgcgt cggaggcgat gaatttttaa tcattgcaac ggaaattcat
    25021 catcccgata acgcagcgca cattgctgag aaagtgattc aaaccgtttc ccagccgatt
    25081 atgatcaacg gtgaacaagc cgtggtcggc accagtatcg gcattgcgct ttatcccgaa
    25141 cacagtgaag aaatggaccg gctgattaaa ctggctgacg aagccatgta taaagtcaaa
    25201 aacgccggca aaaacaactt ccgttttgta aatgacgtaa attagttttc cttccatgag
    25261 ccgcaccgtg acgtaaaggc gtttcctagc cggcagctcg acggctgcct agtgaattcg
    25321 gatatagaga tgatggatca gcgccgcctg aatcatcggc gatgccggat gttgattctc
    25381 ccgaatgtgc cggcatgcat gccgccgaga tggattgtcg acaaaattcc cgccggcagg
    25441 gaagcaaggc aacgtgcctg aagccggtga tttccaaaaa tcaattttaa agaggctgtt
    25501 cgtgcctttc tattttatca ggcgtaaaaa gagcactctg actcatgcgg attttcggac
    25561 attccttcat cagcaagccc gactgttttg ccagatcgat cgtctgtaaa aaatctttcc
    25621 ttgacacatg cgccaaaggc tcgaccagaa aaaaccatcc ctgcggtttc atcagcttgc
    25681 ggatttctga aaaaaatttt ttcaggtcgg gaacctcgtg cgccatccag aatgccagga
    25741 taaaatcggc tttttcacga agggcaagag aatccggctc ggccagatgc gttctgatga
    25801 ttgcggcaac gccgtgcttt tgagcgcgcg cggcaagcgc ggaaagcatt ctgggttgaa
    25861 tatcaacggc gataacttta cccgcagggc cgacgagttt ggccagggga atcgtaaaaa
    25921 agcccatacc cggaccgata tccactgccg tatcgccccg gcggacgtaa gaaccgagaa
    25981 tcgtaacggg gtcatgaaaa attcttcgca taggattatc aaacgtaaaa cacaaccacc
    26041 aggggcaaac atgctttttg ttgaacggtt ttacagtcat ggtttttcaa aagacaattt
    26101 tcagagaaga tttttttatt ctttacgctt atttttagtg aaattcaaca gggattgctg
    26161 cgatccatga aaacaatggt tcgtctggaa atattccttt gacactctgc gctcttctcc
    26221 ttatactttt atcacaggaa ggacatatgg tgaatggagg atgttgatgt cgccggaatc
    26281 catatcgcaa acgctgaccg gactgcgggc attttttgac gcggggaaaa cacaggacgt
    26341 tgatgctcgc atcgcccggc tcaaaaagct caaggaaggc atccgccggt acgaaccgga
    26401 gatcatggcc tcgttgtacg acgatctgcg caaacctctt tttgaatcct atgccggcga
    26461 aatcggcatt ctttatacgg aaatcgatca tgtcatccgt caccttccgt catggtcacg
    26521 ctcccggcgt gcggcgacac ccctcattca tttcttgtcg cgaagccgta ttgatcagaa
    26581 accttacggg gttgtcctga ttatcggtcc gtggaattat ccatttcagt tggtcatggc
    26641 tccgttaatc ggtgccattg ccgcgggcaa ttgtgcactg attaagcctt cggaattttc
    26701 accggcaaca tcggcaacga ttgaaaaaat gatcagcgag ttgtttgatc cgggggatgt
    26761 cacggttttt cagggagacg ctcaggccac gcaaaaactt ctggaggaga aattcgatta
    26821 tatctttttt acgggaagca cagccgtcgg ccggagcatt atgaccgcgg ccgcccgcca
    26881 tctcactccg gtaaccctgg aattgggagg gaagagcccg tgcattgttc acgaagatgc
    26941 cgctcttcag tacggaatta aacgaatagc ctgggggaaa tttttcaatg ccggtcaaac
    27001 ctgtatcgct ccggattatg ttttaattca tcggaaaatt aaagccgact ttaccgagaa
    27061 aatgatcagg acggtttttt cattttacgg cgacgatccg atgaggagtc cggattacgc
    27121 ccggattatc aacgaacgtc acttcaaccg tctgctcgag cttttgaaaa gcgggcggat
    27181 tctgttcggc ggaagatatg cccgtaaaga tttgtatata tcgcccacca tcattgaccg
    27241 ggtttctccg gaagagcctc tgatgcagga ggaaattttc ggaccgctgc ttcccttgat
    27301 tgaatatggc gatatcgacg aggcgattgc ctttatcaat cagagaccgc agccgctggc
    27361 gctttattgt ttttccgagg acaaagaaat tcaggaagag gttctgcaga aaacatcgtc
    27421 cggaggcggc tgcatcaacg ataccatttc gcatatcgga agccaggaac ttccgttcgg
    27481 cggtatcgga gccagcggta tgggcgccta tcacggcaag gccggtttcg ataccttcac
    27541 gcaccggcgc ggcattctgt ttcgctcgaa tctggtggat atccccttgc gttacccgcc
    27601 gtatcgtgac agagtatcaa tactgcaatt attgttcagg ctgatccgat agaatgttct
    27661 ttgtctgaaa aaccgctgcg ctttatgggg cagctttttc cgggaggtga ctcatggctg
    27721 aaaaaacaat cattatactg ggcaagtccg ttaaaaattt taccctggcg gatcaattcg
    27781 gacaagattt tatgctttcc cgaatgacag ggcgacgcgt gcttttatct tttcatccgc
    27841 tggcgtggac gccggtctgc acccggcaga tgcagtcgct cgaaaaaaac aaaaaagtat
    27901 tcgataagct caataccatt gccgtcggtt tgagcgttga cagcgtcccg tccaaagcgg
    27961 cctgggcgaa agccatcaag gtaaaacata ccaaacttct ggctgatttc tggccccacg
    28021 gcggcgtggc caaaagtctg ggattgtttc gcgaccaaaa tggtttttcg cagcgcgcca
    28081 atgtcattct cgatgaaaaa ggcaaagtgt gttttgtcac agtctatcct gtcgccgact
    28141 taccggacat caacgaaatc attgcttttc tgaaaaacgc ataatcaact caacctcccc
    28201 gttttccggc aagacagaat ggggaggtta tctattcatt tgatattttt caataaatat
    28261 ttctttgaca cacaccggcc ttactcatat actgccgcca cagattgtga aatataaaaa
    28321 aaggagaaaa tgaatgatta cactcattgc gacgttgaaa gtcaaagacg gcaacatgga
    28381 cgaggctatt caagtcctga aagagatcgt cccgcaggtg aaggcgagcg agcccggatg
    28441 cctggactat attccgcata cggtccgcgg cgatgacaag actattattt tttacgaaag
    28501 atacagcgat aaagatgcgt tgaaactgca ttcggccaat ttgatgaaaa atatggagaa
    28561 acttttcccc ctgctggaac cgggtatgga cgtcaaaacc tgttatgaga tcatccgctg
    28621 atcctgtttt tctgtcacga cagtttttgt atccccgtaa ccatagagca ggcattttgg
    28681 agcattaatg gatgtaagac gaacgatcaa cgcactcaag gacgtaaaca ttgccaaaat
    28741 gtttacaacc atcgcccgcc ttctgcattt ccgcctgacc tggaccagac gcgatctgga
    28801 ttatctaccc gccggtctgg ggccgaagtt tgtcagcgcc cgccgggcgg cggccatgat
    28861 ccctgacgga gcaacgctga ccataggcgg ttttgccgca acaggacgcg cctccatctt
    28921 ttactgggct ttgcgcgacg catttgatcg cagcggacat ccccgcaatc tgaccgtgat
    28981 tggcgcctgc ccgcagggcg gccgcggcaa aacaccggga atgatcgagg aacttgatgc
    29041 gccgggaatc atcacccgct atatcgtcgg gcatggagaa accgccaaag cgcttctgca
    29101 actggccgat gacggccaac tcgaactgca caccatgtca cagggagccc tggcgttttt
    29161 gatcgaggcg caggcccggg gacttgcctc cattcagacc tcggtcgggc tggacacctt
    29221 tctcgatccc gctgtcgggt gcggttccgc ggtgtctccc ggagctccaa acaatttcat
    29281 cacggcaaaa aacggaaaac tggaatatcg tattccaccc atcgaccggg cgctttttct
    29341 ggcgccttac gccgacaagg acggcaacat ctattttaaa gatgctgctc tgattctgga
    29401 ttacaaagat gcggccgccg ccgcacgcct caacggcgga aaagtgattg tctccgtatc
    29461 caaaattatt ccccgggaca attcacagat cgccatcccg gcggacaacg tcgatgcaat
    29521 cgttgtcaac ccgcgcaatg aacaggtcgg cggaattctg caaactcagt tcatgccgca
    29581 ttttacagcc ggctcgtccg aagtcgatca cgaagccgtc aaactcatgc ggttaattaa
    29641 tacctttctc gatattacac cgtatcgcgg caaggtcgtc gatatcctcg cccgcctgtg
    29701 cgccgacacg ttcgtgaaga cgaccggtcc cggggccatc gtgaacatcg ggatcggcat
    29761 cggagaggaa gttgcccgaa tgctttatga atccggtctg tacagggaca tcaccttcac
    29821 ctcggaaggc ggcgcctatg gcggccttcc ggcatcaggc gtgtacttcg gcgcagccat
    29881 cagcccgcag cgcatcctga gcactgcgga aatttttcat atctatgaag aaagacttga
    29941 tacctgcgta ctcggttttc tacaggtgga ttcagccggg aatgtcaatg tctcccatcg
    30001 tggaccgaaa attattgatt tcgtgggacc gggcggtttc acggatatcg tcgaatatgc
    30061 ccggaccatc atctttgtgg ggaactggat ggatgaagcc cggtacggat taaagaatgg
    30121 acttgtccgt gttaaaaaac cgggcgttcc gaaatttgtc gacaaagtgg atcagattac
    30181 cttcaatgcc cggaaagctc ttaaagcagg ccggaaagta ctgtatgtaa caacggttgg
    30241 cgtctttaaa ctgacggagg aaggtcttga gctcgtccaa attatgcctg gaattaatat
    30301 cgaaaaagat attctgcaaa acagcaccgc tcgttttatt attccgggtt cccccatccc
    30361 cgaggtggcg ccggaaatcg tcagcggcaa aagatttgaa cttaagttcg ataaaaagga
    30421 cctcgcctga acctgcggac gcaaaaaaat cggacatttt ctttaaaaat gtccgattcg
    30481 tctgtttgat catctcgctt agggcgctta ctggccggca tttttgacat cggttgccgc
    30541 atcgtttacg gcggatttga cttcaaccgc tttctctttt acggcttcct tcgcttccgt
    30601 accggcgctc ttgacgtcaa ccaccgtctc ttttgcagct tccttcactt cttggccggc
    30661 gcttttgaca tccgtggcaa ctttgttggc caccttgttg gccacttttt cgacgtcttt
    30721 tttaacctgc tttactttca ctgtagcctt tttcgccatc tctttggctt ttaattccgc
    30781 ttcggctttc atcgcctcgg cctgggcttt taaatccgcc tccgcctgct gcgcatcagc
    30841 ttgtgctttc atttcggctt cagccttttt cgcctccgcc tgcgctttca attcagcagc
    30901 ggctttctgc aagttcgcag agtcttcttc aattttactg caacccgtca cggctaaagt
    30961 taaagaagcg ataaagaaca gagaaatgaa aatagcaaat atttttcgca tcaactggga
    31021 acctcctttc aagaatttag aacctggctt catgttccaa gtttttgtat cgatggaacg
    31081 ctatacgcgg cagggagctt tgtcaagagt caaaaataag aattttcatc aggaacggac
    31141 ctgtccgttt ttatacatct ggatgaaaac ctcggcctcc cgatatgctg cttattcaaa
    31201 tctcaaagca tcgatcggat tgagcaaaga ggctttgtaa gccgggtaga aaccgaagaa
    31261 tatgcccacc agaccggaaa aacaaaatgc cagcagaacc gatggaagcg atacgacaga
    31321 cgggatttca aaaacattgg acaggatgaa ggaagcggtg ccgccgatga tgatgcccgt
    31381 aataccgccg atcatggaaa gggtcagcgc ctccacaata aactgaatgc ggatatccca
    31441 ggtcttggcg cctacagcca tgcgaatgcc gatttcccgc gttctttccg tgaccgaaac
    31501 cagcatgatg ttcatgatgc cgatgccgcc gaccacaagc gagacagagg cgatcgcacc
    31561 caacagcagg gcaaaggtgt tggctgtctg ctccgccatt tccagtacct gcgtcaaatt
    31621 catgaccgta aaatcatctt cccggttcgg cccgatgcga tgccgctgac gcagcagatc
    31681 cgtgatctgc ctttccgcca cggatagttc ttcgctgctt cttgccttga ccatgatcga
    31741 ccgcaccatt cccggaatgg ttctcccgaa gagttttttc tgtgcggtgc ttagcggaac
    31801 aatgattgta tcatcctgat cctgaccgcc cagagactgt cctttggatt cgagcacccc
    31861 gatgaccgta aagggaattt tcctgatgcg gatgacctga ccgagcggat ccatactgcc
    31921 gaaaagatta tccacaaccg tctgtcccag aaggcatact tttgtggcgc tgcggatatc
    31981 ctggtcggac atgtttcttc ccgccgacaa accgcagtcc ttgacttcca gaatccccgg
    32041 cgtcgttccc tgaatgccgg tagaccagtt ctgattcccg aaaaccacct gggcggtgcc
    32101 gaactgcatg ggagccacgg cggcaaccgc tgaacattcc cgcaatatgg cctccgcatc
    32161 gccggtggtt aatgtggatt gacttccgcc acccattctg acgcctccct gcgtggtgct
    32221 gcccggcatg atgatgatca gattgctgcc gatactggag atttgagagg atattttccg
    32281 gctgaccccc gtgcccaccg ccagcatgat aatcacagcg ctgacgccga tgatgatgcc
    32341 cagcatggtg agcgccgacc gcattttgtt gacccacaac gcccttaaag caatgcggag
    32401 tgtagaaaaa atactgatca tgatttttgc acctcctcat cgctgaccac atgtccgtca
    32461 acaaaccgga tgatgcgttt actgaagtcg gcgatatccg gctcatgtgt aaccagaata
    32521 atggttgtgc ctgaatcccg gttcaactga accagaagtt ccatgatttc aatgctggtt
    32581 ttcgtatcca gattgcccgt cggttcatcg gccattaata taggcgcctg attcacaagc
    32641 gcgcgggcaa tggcaacgcg ctgttgctgt cctcctgaca gttgattggg atggtgatac
    32701 gcccgttcct ccagacccag catttttaac gcttttaagg attcctcttt gcgttgcctt
    32761 gccgggacgt gattataaag caaaggcaac tcgacgtttt ccagcgccga cgttctggac
    32821 agcaaattaa atccctgaaa gacaaaaccg attttctgat tgcggatcgc cgccagatcg
    32881 tcccgtgaca gaccgcttat cgtttgtccg tccagaaaat aatcccccgc agtcggctga
    32941 tccagacacc cgatgatatt cataaaggtg gatttgcccg aaccggacgg ccccatgatg
    33001 gcgacaaatt cacttttgtc gattttgacg gagacgtttt ccagcgcgtg aacgccttca
    33061 tcgcccacat catacgtttt gacaagctgc cttgtttcga tcagagtcat cacataaacc
    33121 tcggaggcgg gcgctgtgtg gtgctgctgc cttttttatt caggctgtcc gtgacaatct
    33181 cctggcccgt gcgcaggtcg cctttcacaa tttcagaaaa attcccgtcg ctcaatccga
    33241 gcgtaacgcc gacgggtttg ggctgattgt tttccagaac ccagactttc gtccctttga
    33301 tgtcctgcag ctctttcgct gatacgcctt tttccccgat ctccgcgggt ttaaaacgaa
    33361 gagccgcatt cggaacccgc aggacatccg gacgcgtttc tatggtgatg gaaacattgg
    33421 ccgtcatccc cggctttaat tttaactcgg gattatccac cttgataatg acgtcgtacg
    33481 tcaccacgtt ggagacggtg gtcggcgcat tgcgcacctc ggaaaccttg ccggaaaagt
    33541 ccgtgtccgg ataggcatcc accgtaaacg agacgtcctg tcccgcccgg atctgaccga
    33601 tatccgcttc atccacactg gcgttaatct gcatttgcgt gagatcctgg gcaatgctga
    33661 aaagcgtggg cgtctggaaa ctggcggcca ccgtctggcc gacatctacg ctgcgggaaa
    33721 tcaccgtgcc gttcaccgga gaaataatat tggtgtactg taaattggtt tccgccattc
    33781 tcagagaggc ccgcgcctgc gcaacttgcg cctcgctgac tttctgctgg gcttgtgcgg
    33841 catgcaaagc cgtttccgcc gtatcgagat cgctgcgtgc gataaagttc tgggaaaaaa
    33901 gttccttgtt tcgttcgtag gttcgccggg cgtcaacgac ggcaaccccc gctttctcca
    33961 gattggcctg ggccgaaagc aggtttgctc tggcctgatc cacctgcgcc cggaacgtgg
    34021 ccggatcaat ctgtgcgaga agttgcccct ttttcaccgg cgaattgaaa tccgcaaaaa
    34081 gagttctgat ggttcccgat acctgcgtgc cgacagaaac agtcgtgacg gcattcacgg
    34141 tgccggtagc ggtcacaacg gatttgactt caccgcgcga aattttttcc gttttataga
    34201 cggctttttt cccgtctctg ttgacataaa ataatatccc ggccgcaaag gcggcaatca
    34261 gcaatacgga gatgacaatg attttcttct tcatggtttt gctcctatgg atttttccag
    34321 aacggcctgt gcgatcctgt aattacaaag cgccgtattt aaaccggctc tcgcgttatt
    34381 gagcataatc gtcgcgtcgg caacttcaat gggactgccg acgccgctcc gataacgccc
    34441 cgacgccaga tcgtaatttt cctgagcctg aacgaccgtc atttcagcca tggcaatttg
    34501 ttcagcggta tcctgaacat tcagataggc ctgctgaaca tccaagtgaa tggcctggcg
    34561 gacggtctct tcgttggctg tcagtattgc cagattggct tcggcttcgt caatctgata
    34621 gttggtcgaa aaccccgaaa aaagcggaaa actgagagac gcgcccgcgt tccagccgcg
    34681 gcccagcgga aaatcacggc cggaaaagcc gtatccggcg ctccccgaca gggtcggaag
    34741 ataatctttt ctcgccagat cgatcgaccg ctcggctgcc tcgcgtctgg ctataatcga
    34801 aagaagatca ggacggacgg catacgcctt gttcaaaata tcgtccagga tccatgcctg
    34861 tttctgaaaa gacaggttat cgacaattgt aaaatcggcg ttttccggaa tgcccatgac
    34921 gtccttcaac gtgatccgtg cagcacgaac ggcattttcc gccttcaaaa cattcagccg
    34981 cgcattgcct aaatcgactt ccgctttggt cacatcgaat ttcggtttgg tgccaacgcg
    35041 gaaaaaagcg tttgcctgat ccagatgctg ctggaactgc tgcatcgtat cgacggccac
    35101 atcccgattg cgctggttct gcaaaagcgt gtaataagct tttttcacat tcagaatgat
    35161 ttgcgctctg acatcgtcat aatcggcgcg cgacgcgttt acgttcaaat cctgaatggc
    35221 gacatttgct gacgtcttgc cgaaatcgaa aagggtcacg cccagattga tccccgtcga
    35281 atactgatcg tacgaatcaa tggaatcgcc gggcggcgtg gtcggaccga tcttctgata
    35341 gccggcggac acatccagcc acggatagta tttggacctg gcctgcccga cccggctttc
    35401 cctggcccgg atgtcgccgg cggacgcctt gaggctggga tgcttgtcca gagcaacggc
    35461 aatgcactga tcaagatcat acgaggccgt tccgcctgcc ggctccgaag cggcggcaaa
    35521 cggaatcaag acgattaaaa aaaggagcat tatctttttt tgcatttttt caccctggct
    35581 gattattgcg ctttcctcag gcctcctatg gaaaaggagg cgatgtgttc ggttatttca
    35641 tcgatctgtt ccgccccgaa ggttgcttcc ggataaattc tggtcatgat cggctgcgaa
    35701 tggcggaaat aaagacattg accgacaata ctcattgaac acagccgcac cattttttcc
    35761 cggggcgttt tgcccagcac gtcggcgacg atttccgtaa ggagttggaa agaaggacgg
    35821 atggtctcgt tgaccagaac atccagcgcg caggtcggtt cgctgaattc ccgcgccagg
    35881 agcctgccga accagacggg acgtccgtca tccataatcc gtttcagaaa catccggata
    35941 taaattttaa gcttttcctc cggtttctga gaggcccgga tacccagatc gggcggatac
    36001 gcctgaaaag ccatctcctg ataatacttc aacacggaca gatacaatcc cttcttgtcc
    36061 ccgaaataat aattgaccgc cgccagattg gccccggcgc tgctgcaaat atcccggacg
    36121 gtcgccttga ggtaaccttc ttcggcaaag attttccctg ccgcctcaat gatcgcggtg
    36181 cgtgtttttg aatttgtttt cccggaggct aaaaccattt ttcaaacacc tgttttaaac
    36241 aagcgtatga atattgaacc gctttgtcaa ggtttttttc acaaaaacga tcaacgtccc
    36301 gtttttcggg gcattttttg acaacagcgc ctatttgtca tatacagtta aaccgcttaa
    36361 aaaattttct tgaacgtccg gcagttaagc ggatttcaat cacctgcccg gccttagacg
    36421 gagacaacgg agtgaataca aaaaacatgc tgcgatatat ttccgcttta acggttatcg
    36481 ccgctgcagt ttactttttt tacctgcaat tcaaaaaaaa cgccgacgcc atcggcgcct
    36541 ggaagttcag cattaacccc ctggagatga gcctggcggt aattttcggc agttgcgccc
    36601 tgcttgtcgg acctctcgtc tggcgacttt ttgtcaacag ctatttgaag aaaaaattaa
    36661 actatactga aagctttgtg ctttactgca catccgccat gttcaaatac atcccgggaa
    36721 aaatatggac ctatgcggct cagatagccc tgctttcatc caaaggaatt tctaaggtcg
    36781 cattgcttta tatcaatctc gtcagcttta tctgtctgct tttcgtcgcc gtgccgttct
    36841 tcttgtacta ctatttcatt tacgttcagg aaatgtccct cggggcaact ctgctgatct
    36901 taacgctgtt tatgctgatg gatgttgttt tcattatcgc gaaccatacc atcgtcaatt
    36961 atctgattcg gcccatcaac cgattgttca agttgaacat caaaccgatt cgaaccgaca
    37021 aaatcatttt tgtctatgta cagatgcttt actcgctggc gtgtgttttg ctcgggatcg
    37081 ccctgtattt tcttgcccgg ggagtaaaca tgccattacc gttttcgcag atcattgcca
    37141 tgatggcgac aatctgcgtt tcgctgattc ttggattgct ggcttttttt accgtgggcg
    37201 gactcggcgt gcgggaagga gccatgtttt tcatgctgaa gcagttttcc aatattgaag
    37261 cggccctgat cctcccggtg atctcccgat ttctcataac ccttgttgaa cttttaatgg
    37321 ctgttaccgc aataataata ggtttaaaat acggctattt tcatgatata gaaaaagtga
    37381 agccagcagc cgactccgca ttaccgcgcg agtcggctgg atgatcaccg gacagttttt
    37441 taacaacgga ggtaataacc atgatgacct ttacggattt attaaaaaac agaaggtcca
    37501 ttcgcgattt tcaggacaag aacgttccca tgaaccttat cgaggaaatc cttcaggaat
    37561 cgacgttggc gccttcggcc agcaacaatc agccctgccg gttttccatc gtccattgca
    37621 gaaaaaccat caaggcgctt tccgatgaaa gcaaagcaaa tctcctggag gattacgccg
    37681 gcaataaaaa atctctcctg ccgcagtata tcgatatgct gaaagatgaa aatttcaatg
    37741 tcttctacaa cgccccctgc gtgattttca tcatcggttc aaagagcgtc aagtcgcttg
    37801 acgtggattg cgcgctggcg gcaagctaca ttatgttttc tgccgtcgag cgcggactgg
    37861 gaacctgctg ggtcaatctg ggatccaaca tccgggatcc tcagtccagg gccctgctgg
    37921 ccattcccga cggctgccgg attgtcgcgc caattattct cggttatcca aagacaattc
    37981 cgccggtatc cgaacgccac gcgccggaaa ttctaagggt gatcagttaa atgaaataat
    38041 gtatcattcc cgcacaggcg gtaatccagg cgccgttccc gcgtaagcgg gcggccaggt
    38101 ctttatacaa ctggattgcc cgctccagcc tgacaactga ataactcgcc aatattgaga
    38161 tttgcttaga gaccattgac tattgttttt aacccatcga ctggatcttt tttgaaatgt
    38221 atacggatga ttcttcgccc tgcttccgtc agcgcctgcc ggtcgccctt cgcctgcgcg
    38281 gctttgagcg cgccgaacgt gagggaggat ttttcttctc cgatattgtc cggaatatca
    38341 acgtctaaag aaaaatcctg cgtcaactga ataaacaagc cgtttccggc atcgcccttg
    38401 tgcaactgcc cggtggaatg cagatagcgc ggaccgtatc cgcaggnnnn nnnnnnnnnn
    38461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38521 nnnnnnnnnn nnnnnnnnnn nnnnnnttcc cagatgtaca tttgttgacc cagctcataa
    38581 tcgtcgttaa ttttaacggt aatgacggga tgaccgtcct ttttcagagt ttccatttga
    38641 gagggctctt cttcattttt attttggaaa ataacaaaaa tccggtcttt cccatatcct
    38701 gaagcatcca aaagcgtttc gttcagcaca gggagaattc ccctgccttc cttgcctgtg
    38761 ctttccgcaa tcagttgttc cagccagtcg ccaaaatgtt tccagtgcgg cggcaggaca
    38821 aacgtcattt tgttgcgacc ctggttggcc agcattccta aagcggcgcc cagagaagcg
    38881 ccggcgctca tgagcgcttc caaattggat gaaacgtttt gcagcagcgt ttccacagca
    38941 atgccgatca atgccgccgg aacgattccc ggcagcgaca gcgccgagta acgtcctccg
    39001 atatcgggat tgttgaggaa aatatgacgg gcggagatac tttcggctgt tttgatcagc
    39061 ggacttccct catcggtgat aaaaatgaac cggcgtcccg cttcgccgcc gaattttttc
    39121 agacacaggt tataaaaata tttgaagagc gatgttatct ccagggttga acctgatttt
    39181 gatgaaacaa gaaataatgt tttttcaaga tttaaatgtt gtgtaacttc agaaataaaa
    39241 acgggatccg tcgtatcgac gacatgcaaa cggggatagc ccggtttgct gccgaagatg
    39301 ttgccgaaaa cttctgcggc cagcgatgac ccgcccatgc cgattaaaac gatatcgctg
    39361 attttatctt tttgcagggc atcgacaacg ctgcggatgt catttatttt tgccagcgtt
    39421 tcagccggag cgtcaatcca tcccaggcga ttgacgattt catcgggccg gaatttccag
    39481 agggtataat cttttgaccg gatgcgttca atgacgttgt cacggcacat tttatccaag
    39541 gcgcttttaa cggcggcttg atactctccc agagaataat caatgcgtgt ttcagtcatt
    39601 tagggaaact ccaatgtaat gagactcgct aactacacga agcgttagtt gtagagacgc
    39661 tcgctaaaaa aagcgtagcg cagtttgaag cgttagcgga actatccaat cccgatttat
    39721 cgggattgga aactatcccg tcaggagcga tgaagcaagt ctcttgccgc ttcaataatt
    39781 ttttcaacgg taaagccgaa tttttcgtaa agaaccggtc cgggtgcgga agcgccgaat
    39841 gtctccatgc cgatgatttt tccgttcaaa ccgacatagt gttcccaccc cagcttgatg
    39901 cctgcttcca cggcaatgcg ggcggaaatc tgcggcggaa gaaccttatc gcgatagtcg
    39961 cgcggctgac ggtcgaatag ttcccaactg ggcatggaaa caactctggt tttcgtgccg
    40021 gcttccgcaa gcttgcgggc tgcggtcagg gttagtgaaa cttccgaacc ggtggcaatc
    40081 agaatcacgt cgggatcgga cgcggattcc cacagaacat aaccgccctg tttggtgccg
    40141 gacgcggatg cgtaaacgct tctgtccagc acgggaaggt tctggcggct gaagatcagt
    40201 gtcgtcggcc cttttatatt ggtaatggcg attttccagg cctccagtgt ttcattggcg
    40261 tccgccggac ggattaccgt catgttgggt acctgacgca gattcataat ttgttcgaca
    40321 ggctggtgtg tcgggccgtc ttctccgacg ccgatgctgt cgtgcgtaaa gacgaataca
    40381 gcgcgcaggc ccatcatcgc cgccaggcgc atgggcggac gcatgtaatc ggcaaaggtc
    40441 agaaaggttg ccgtgtaggg aatgatgccg ccgtgcaacg ccatgccgac ggcaatcgag
    40501 cccatcgcat gttcgcgtac gccgaaatga atattacgtc cggagtaatc ccaggcgccg
    40561 ccgaccatgc cgtgcagccc ttctttggat aatttaggac tctgaaaatc gcccatgcct
    40621 ttgagccagg taaaacagga cggattcaga tcggcggagc cgcccgccag ttcgggaatt
    40681 ttgcctgcga tggactgcat cacgatttcg cccgctttgc gggtggccac gtcttttgat
    40741 ccgttgggga attcgctcaa agccgattcc cagccatcgg acaggtcgcc tttcatgatg
    40801 cggtcaaatc ccgccgccaa agcaggttca ttttgtctga agttgttaaa cgattcctgc
    40861 catttttttt cgtctttttt gccgcgcgac acagctttgc ggaaaaacgt ataaacgttt
    40921 tcgggaacag aaaaaggaga atcggccagc cagcagagag cctctttggc cttctgcagc
    40981 tcgtcggcgc ccagcggtga gccgtgcgca tcgcagcttc cctgtttgca gggtgcgccg
    41041 taaccgatcg tggatgcaat gcaaatcaat gaaggctttt cagcttcgcg cttggctttt
    41101 ttaatggcct tgtcgatggc ggcaacgtcg ttgccgtcgg cgactttgat cacctgccag
    41161 ccgtaggcct taaaacgctg ttcgatgttt tccgtagagg aaagggccgc ggatcccgcc
    41221 agcgtcactt tattatcgtc atacagaaca atcagttttc ccaggcgcag atggccggcc
    41281 agagcgcagg cttcaccagc cacgccttcc atcatatcgc cgtcaccggc catgacatag
    41341 gtgaaatgat cgactatttt ctgatcttcc ccgttgaaac gcgcggcaag atgcgcttcg
    41401 gcaatcgcca tacccaccgc attggccagt ccctgaccca gcgggccggt tgtaacttcg
    41461 acaccgggca catgtttgta ttccggatgc cccggcgtgc ggcttcccca ctgccggaaa
    41521 gttttaatat cgtcgagcgt caaatcgtaa ccggtcagat gcagaagagc gtataaaagc
    41581 atggaggcat gaccggcgga aagcacaaaa cggtcgcggt cgggccattg cggatttttg
    41641 gggttatgtt tcaggtgttt tgtccagagc gtaaacgctg cggctgctgc gcccagcggc
    41701 ataccgggat ggccggattt ggccttttcg atcgcatcgg cagcgagaaa gcggatggca
    41761 tcaatacatt gtttctctaa ctcgttttta tcagacatga agactccttt aattatcgta
    41821 aggatggtga tgtactaaca catttgaagt tttttttaaa cttttcttgg aataaatttt
    41881 accgcggtgc acggaaaatg attgcaaaat aaacatttag agattatgca ttataaacaa
    41941 taagttagct tatgtctaaa ccaatgattc aactcatcaa aataaacggg ggaagttatc
    42001 atatatgaca atttccgcat ttgaaccggc tacattggca ggaatcaaat tgaaaaaccg
    42061 gataatccgt tcggcgacct atgagggcat ggccgatgaa aaaggattcc ccaccgaaaa
    42121 actaaaaaaa ctttacatca atctggcaaa aggggatgtc ggcgcaatca taaccggata
    42181 cgccgctact caggcggacg gcaaggtcag caacgccatg gccatggtcg atcatgacga
    42241 caaaatcaat gtttttaaag aaattacaga tgccgttcat gaatacggca cgccggttat
    42301 catgcaaatc gcccactgcg gcaggcaaac ccgctccaga ataaccggtt ttccgaccgt
    42361 tgccccctcc ccggtgcgca atatgtattt catggaggat aaaccgaaag ccttgtccga
    42421 agagggcatc aacgaaatca tcgacaattt tgttgccgct atcgtccggg caaaacgagc
    42481 cggatttgac ggggtccagc ttcatctggc gcacggctac ctgctgtccg attttttatc
    42541 ctcgcatgca aatcgccgta aagaccgctg gggcggctca acagaaaaca aataccgcat
    42601 cgtcggcgag attttcaaaa gagccagggg aaaagtcggc gactacccga tccttgtaaa
    42661 aatgaacgcc tatgacgggc ggcgaaaagg catgagaatt gaagaagccg ttgtcatcgc
    42721 ccggatgctg gaaaactccg gctgcgcggc catcgaagtg tcctgcggtt gcgtggagga
    42781 cggccttttc accatccgcg gagaaaagct tcccgtggaa gccgtcgtgg aatacaattt
    42841 caatttcaaa aattatccgg actttgcgaa aaaagccatc gtctggtttg ccaaaacatt
    42901 tctgaaaacg cccaaaccga tttcgaaata taatctcgac gcagcaatcc aaattaaaaa
    42961 agccgtgaac attcccgtga tcgttgtcgg cggaatcaac aatgtcgcag atgttgagga
    43021 cattatcggg aatgataagg cggattttgt ctccatgagc cggccgttta tcatcgagcc
    43081 ggacattgtg agcaaattca aaaaccggac gcagactaca tcaaaatgca tcatgtgcaa
    43141 tttttgcgtg atcaccatcg agaaagaacc gctgaagtgc cgctacggca aactgccgaa
    43201 aacaaaaagc gcttaaaacc atcttccgga tcagcatgtt gtgcgcgtta aagtttcctt
    43261 gcaataatcg caaattccct gctctgcgca gaaaaaggcg tgcccgccac atcggcataa
    43321 taattttcga tggcaaaacc gcaggcggaa aactccctct ccacatcttc aggactgaaa
    43381 tactgcaacc aattatagat tgtgcgcgta cgctcagcct caatgatcgt gtattgatcg
    43441 aggaccactt tttccttctc atacttgaag gtattcagaa aaccgtaata tttatcggga
    43501 gaccagaagc catcgagaag attgggttca tacagagcct tttcttcccg ttcgttgaag
    43561 gcgtttaagg aatacacatc aaggagaacc gaaccggcgg gctcgagaag ccgatgaaat
    43621 ttggcaagca tcgttttcct ttgagcgggg ctgagggcgc agaaatcgca cataatcatc
    43681 aaaatgaggt caaagcgctc gtccgtttca aaatccagat a
//
LOCUS       DAONQZ010000015        43202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_976_length_43202_cov_13.721302, whole genome shotgun sequence.
ACCESSION   DAONQZ010000015 DAONQZ010000000
VERSION     DAONQZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 43202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43202
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_43202_cov_13.721302"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..841)
                     /locus_tag="PLR13_04330"
     CDS             complement(326..841)
                     /locus_tag="PLR13_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQC18593.1"
                     /translation="MSELFEKVKLGISKSVAKASVKSKEFLDVTKLKNQISSLQNKKQ
                     DALEELGNIAYAMFLKNNIDTDIIKNKGNEISKIDEQIKNLQNEIVEVQKRAQDSLRE
                     DVPSANTCACGAVIPEGAKFCGVCGSKVETASMSGEHPKDPILESRCKQCQTELEPGA
                     KFCAMCGAKVS"
     gene            1094..1822
                     /locus_tag="PLR13_04335"
     CDS             1094..1822
                     /locus_tag="PLR13_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQC18594.1"
                     /translation="MPNIQFFALAESVCKKLNLPPIPFPVRKGRDEIIFNPQGVALDL
                     LLDELEQYLIEKPEEKIFYKDAFSRLAAIEGIRLGEEGFHDHAAHYFELGLALDPANL
                     ALRSNYALSLQSAGRKEEAMRQYRFLLQQPVISGQCLVLIPAARLFLDCGDPVTAHQI
                     VKHCASFMPVDNEFWDLFAEARQRCGIKRWMAPRQKQQEIMRQATAPDYKSAPTGKKK
                     NFCPACGNRIKPEARFCGGCGRAL"
     gene            1812..5192
                     /locus_tag="PLR13_04340"
     CDS             1812..5192
                     /locus_tag="PLR13_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18595.1"
                     /translation="MRCKNDFSVECMKAKAFAVQSVLYAFRVFTPVLILLIILLLFVS
                     HAIADASDYRDKGKENWVLFTFILKDAKTGEPIKGRVYYQSDLAADWFHPDNTKPLLP
                     PGIYRVVGWERHYWQSEQKLIVNPLKGLQQQIVIDLEPVSSPRDGKSGSPLKVPPILS
                     NLIITGNAAVKMPHMNASWFGQSSMATFHVYTNAWSEYPGGIGGGSRNSTGHIDYYFF
                     LHTEDAQKHYERLRPGFKVIPNSSQHVSGPTPVSLGDEALQISIDTERPDGKGLLYES
                     RKYLIRDGHFVMNMYINREIGLDFLTSPEEILKRVRALDVRQVKQMLEPEPYPWPEIT
                     PTPGSGKDVDSLGSKNEVADYASVSPEKALIAVAGVSMVLTLGVLVQLWGMGGLQSPG
                     DIRGALEGLFSSSGGEVTETESSVPETGAVQEVEVIPKEPEALVPETVIQESRVVETV
                     IPDPVPSGFEYQGKVWYKPPWDKGGPYWMDKSDYDAMRSMMRRGMVWSDRWGWVTPED
                     GKTMEAQRVAAWDEYKSNTDKDIKVLTDRIAESQEKLAEIREKQAELERIEQLRERLT
                     ELERQRELDNSFATQLSDTWDNYVEGVNRDLDALPGELGDMALDAARGIRDTTGALIT
                     AAGDTVSDLTNGDNWKAVGKAAVDTAKDLILHPLESSVKVGDVVKDAGSIAIKAADVA
                     GNVAVAIVSDPFAFAESVVGIDNWKKVLDPNVPVGERVSRALYGAVDTALNFASGGTK
                     TAAAGVEAGMDALKTADTVSDAAKAANAADMAADAARITPKLDDPARVQVWEVGRQAG
                     QQKADKLAEAIKSGKPSEIKQALVECQKDKHAIGALNRADDTVKHAYNQQMKQLITDD
                     VDKVVKEKIAQRYGLDPKDVEIVKPTNPTSKPKVGSDLDFTVKVKAKPGEIVPDPNKP
                     GSFIHVKPGDNLTKDVPLKDAQEIYNRELYSKVTGEPIPTDPEKFKAFQAEADKLAHE
                     MDHVAGHRVGAESYGASQQDLEVILQKSGGRFTDPEQAGMVASYKSTHLYQEAESITK
                     LDPGKAEQLVADGMRQTTKQYSNLIETRVAALQNQGIEVAVDPRLTKAVDIFKQVETK
                     GLPPAKAEALLKSQLNMSKDDVANMLGAQLAKLHRVKA"
     gene            5219..5683
                     /locus_tag="PLR13_04345"
     CDS             5219..5683
                     /locus_tag="PLR13_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18596.1"
                     /translation="MWAVFLKIVSYFFLLLFVLGVVLMLLTWRKEKFVKPKWILFGQI
                     VAFMALVVFTMLSRNPLGFWSWLLIFLAGLCGGYFYGRTVKVKKSARGIMMNYTLPYV
                     ITWGVLLFLTQFLTISTGRIPVIVLGLCVLNTGLNLAMNGQVVWNYNRMKKT"
     gene            complement(5747..6934)
                     /locus_tag="PLR13_04350"
     CDS             complement(5747..6934)
                     /locus_tag="PLR13_04350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQC18597.1"
                     /translation="MSIAKKIKSSISNSSMIRKMFEEGAVMKRKYGADKVFDFSLGNP
                     NVEPPAVFKRELIRLAAKPLPLKHGYAPNAGFADTRQAIAKQVSKTCDLNLSADHIVM
                     SCGAAGALNVIFKSLLDPNDEVIVPMPYFVEYPFYIDNYGGVTKFVRTRKDFSLDIEA
                     ISKAINKKTKAVLINSPNNPTGKVYSSKNIQALAALLKKKGKTFHKTIYLVSDEPYNE
                     IVYDRVIVPSVLKSYTNSIVACSYSKTLSLPGERIGYIAVNPEIDDCRDLISALILCT
                     RILGFVHAPALMQRIVVKLQNAAVDVNVYQNKRDLLCKGLSKAGYKFAKPQGAFYLFV
                     KSPLPDDVAFTKILLQKNILVVPGSGFGAAGYFRIAYCVDDKTIVNSMRGFTEAIRET
                     SGK"
     gene            complement(6943..7254)
                     /locus_tag="PLR13_04355"
     CDS             complement(6943..7254)
                     /locus_tag="PLR13_04355"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HQC18598.1"
                     /translation="MSDKKQNVITGGTVLITLGILIYLSKSGSFPFGQTWPVLLIVIG
                     ICTLIQKFRDLGGWIITLAGTGFLFYEFYGFDVSQYSKYVLPGIIILLGIFVLLRRKK
                     G"
     gene            complement(7313..8068)
                     /locus_tag="PLR13_04360"
     CDS             complement(7313..8068)
                     /locus_tag="PLR13_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQC18599.1"
                     /translation="MLGTLSMPTLVVIVLVVMFLVSAIKILSEYERAVIFRLGRIRDA
                     KGPGLIIIIPGIDKMVRVDMRTITMDVPPQDVITKDNVSIKVSAVVYFRVMDANSAVT
                     NVENYLYATSQLAQTTLRSVCGQMELDEILSEREKINMNLQEILDRSTEPWGIKVSLV
                     EVKHIDLPEEMKRSMAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMETQPMSLQL
                     RYLQTLNQIASENNSTTVFPIPIDLFKPFLKDK"
     gene            complement(8098..9381)
                     /locus_tag="PLR13_04365"
     CDS             complement(8098..9381)
                     /locus_tag="PLR13_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HQC18600.1"
                     /translation="MAVFIFAGLLAWAEEKNPIFNVITVDGVITSPTAKYIASGIEEA
                     VRDNAGGLIILLDTPGGMDTAMRDIAKSILNSPVPVIVYVSPSGARAASAGVIITQAA
                     HIAAMAPGTNIGAAHPVAIGLGNSGGEKDKTMTEKIENDAAAYARSIAKTRGRSEEWV
                     EKAVRKSESITAEEALKLNVIDYVAADVNMLLAAIHQKEIQLIGGKKIISTKNAVIRD
                     KKMGTRQGILSAISDPNISYLLLLIGLAGLYFELSTPGAILPGVIGGISLLLAFFGLS
                     TLPVSYTGILLIIFGVILFIAEIKVMSHGMLTVGGIISLVMGSLLLFDTPEPALRLSL
                     QVLIPAVVVVSIFFIVIIMLAVKAQMRKHFTGAEAMIDLEAEVTSDIEKEGDVFVKGE
                     YWKAVSDASIKKGAKVRIVKVDGMKLVVEEIKNQK"
     gene            complement(9427..10146)
                     /locus_tag="PLR13_04370"
     CDS             complement(9427..10146)
                     /locus_tag="PLR13_04370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQC18601.1"
                     /translation="MSNITRNELQAELLHAVRGLKGQVIHNESLAVGVDWSRSSRTVV
                     AKAKTGGQSRGEMLEAIHQEMADCQLCPLGRTRKNLVFGTGNPGARIVFVGEAPGADE
                     DEQGIPFVGRAGQLLTEIIVKGMKLQRKDVYICNILKCRPPGNRNPQPDEISRCEPFL
                     KKQLQLIAPEVICALGTFAAKTLLKTDVPISVLRGRFHSYEGIKLMPTYHPAYLLRNP
                     AAKKQVWEDVKIIMKELNLPY"
     gene            complement(10157..11368)
                     /gene="coaBC"
                     /locus_tag="PLR13_04375"
     CDS             complement(10157..11368)
                     /gene="coaBC"
                     /locus_tag="PLR13_04375"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQC18602.1"
                     /translation="MLENKKIVLGITGGIAAYKAAELTRRLVKAGAHVKIIMTKSALE
                     FITPLTMQTLSQNRVYTDMFASDDEYDMAHIALAEFADAFVVAPATANIIGKIASGIG
                     DDLLSTTIMAENKPTLICPAMNDKMLANPIVQDNIRRLKKNGYAVMDSAVGELACKTE
                     GKGRLPEIDAILAEIENIMTPQDLAGVRILITAGPTEEPLDPVRFITNLSSGKMGYAL
                     ASAAQRRGARVTLVSGPTSLPLPPLEKIVTVRTAREMHKAVLQHYKSARVIIKAAAVA
                     DYRPKIVANEKIKKEGKTLFIELERNPDIIAEIGKKKGNRILVGFAMETQNLLTNARG
                     KLVKKNMDMIVANNLRQEGAGFRTDTNIITVIDRAGKTESLEKMTKIDAAGAILNRIK
                     KLISEKSKGKK"
     gene            11698..13011
                     /locus_tag="PLR13_04380"
     CDS             11698..13011
                     /locus_tag="PLR13_04380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQC18603.1"
                     /translation="MKWFRFDIRILIALVVVAALGWFIFGNYIEIGNPVIKLDSDVKA
                     IGRQKTVGITFSDSKSGLSRITAEIIQDNKGHVLADKKIPQKGVAHESLSLNIASTQL
                     ALHDGPAVLKISATDHSLLKNQTVLSQSVTIDTIPPMINLLKNINYINQGGTGFVAYQ
                     PSKPLSSTGVYINDYLSPAHAVLIDNKPSYITYFALPMTADKAATSIMIFARDEAGNE
                     TRVSVPCVIKEKKFRSDKMHLSEAFLQRKMPEFQAMVPSLQGKTLLDVFTYVNSQMRK
                     ENFSAIQKICMKSAPKELWEGEFLRMSNAAPMALFGDKRSYAYEGKVVGESVHEGVDL
                     AANARSPIEASNKGIVVFTGALGIYGNTVIIDHGLGLFSLYAHLSSIDTTVGKTVAKG
                     EKIGLSGMSGLAGGDHLHFSIMVGGKFVDPKEWWDPHWIKDNITR"
     gene            complement(13039..13905)
                     /gene="rapZ"
                     /locus_tag="PLR13_04385"
     CDS             complement(13039..13905)
                     /gene="rapZ"
                     /locus_tag="PLR13_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQC18604.1"
                     /translation="MKNLRVVIITGISGSGKSTALRALEDVGFFCVDNLPVILLPKFL
                     AITLASSPEIKRVALVMDLRQRSFLDKYSRIFASLKKKGYKIEILFLESSDESLVHRF
                     SETRRVHPLLERGIIMEGISLEREKLSSLKKMADKVIDTTSFNVHQLKDVVQRSFSVF
                     SKNKRMVINVQSFGYRYGLPVDADLVFDVRFLPNPYFIEKLKNFDGNNRNVQNYVLRN
                     RESKQFLEKIKDLMNLLTPLYEKEGKVLLNIAIGCTGGKHRSVVMANKMSAFFTSLKY
                     HVNVNHRDVNKH"
     gene            complement(14030..14473)
                     /locus_tag="PLR13_04390"
     CDS             complement(14030..14473)
                     /locus_tag="PLR13_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQC18605.1"
                     /translation="MEFINAGLEAKNKDEVLAELVNVIIEGGLKLNSSSIIDILKQRE
                     NLGSTGIGEGVAIPHGKISNLNDIVVAFGRSEKGIAYDSLDGKPVHLFFLLLAPENSA
                     GQHLKILAKISKMLKDVYFRNKLIAAKSRDELYQIIIDQERTCIV"
     gene            complement(14565..15098)
                     /gene="raiA"
                     /locus_tag="PLR13_04395"
     CDS             complement(14565..15098)
                     /gene="raiA"
                     /locus_tag="PLR13_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQC18606.1"
                     /translation="MMKISVTFRNGEGENWQKNYAEEKMQKLKKYLDAPAEAHVVVSM
                     EKFRNVTEINLNSNGWNINAKEEAKDMRVALDRCVEKIEKQLKKQREKIRQHKPKTIR
                     RAQEKPLNEEEIEEPTASKIMETRKVILKPMSFDEAIMEIEETNDRFVLYRDTSSENV
                     SMIYRGDDGRYTLIETK"
     gene            complement(15359..16843)
                     /gene="rpoN"
                     /locus_tag="PLR13_04400"
     CDS             complement(15359..16843)
                     /gene="rpoN"
                     /locus_tag="PLR13_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQC18607.1"
                     /translation="MAFELKQHLKLSQQLIMTPQLQQAIKLLQLSRQELVEAINQEME
                     ENPLLEEISTDENSDEALIGEFENDGLPVERDTIKAVEHTEELNVEKGAGTDEFDWAS
                     YLEDYGPVGMTYGGKDGEETSWDNVLTEGQTLSKHLTWQMKLSSFSEDEERVGSQIIG
                     NLDPNGYLCATAQEIAQLENVSEELVERVLQKVQEFDPPGIAARNLQECLLIQARMLG
                     VKNRIIEVIIRDFLKELELKNYAQIAHKLKVPLKEVEMAVLLISEMNPKPGSIYSEDK
                     AQPIIPDVYVVKTGDEYKIILNDDGLPRLRISNFYREVMAGISSHSHEEAENGKKYIK
                     DKVQSATWLIKSIQQRQNTIYKVAESIVKFQKEFFDKGIDYLRPLILRDVANDIEMHE
                     STISRVVNNKYMHSPQGIFELKYFFGSSIRTTTQGTIASKSVQEEIRQLISSEPPRKP
                     YSDCEMVQLLKAKGIHIARRTVAKYREMMGILPSSKRKKYFKAI"
     gene            complement(16867..17589)
                     /gene="lptB"
                     /locus_tag="PLR13_04405"
     CDS             complement(16867..17589)
                     /gene="lptB"
                     /locus_tag="PLR13_04405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQC18608.1"
                     /translation="MSDLSAKGLTKIYNKRKVVNQIDLLIAPGEVVGLLGPNGAGKTT
                     TFYMIVGLVKPDAGEIYLDGENITGNPMYRRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLDLSEAERQERLRELLKELDLTALAKNYAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQKIIEKLKAKGIGIIISDHNVRETLSCCDRAYIVNNGEILVEG
                     PPDTIAQSELARKFYFGDSFNL"
     gene            complement(17599..18162)
                     /gene="lptA"
                     /locus_tag="PLR13_04410"
     CDS             complement(17599..18162)
                     /gene="lptA"
                     /locus_tag="PLR13_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HQC18609.1"
                     /translation="MIRKHTYIFLMLFFFFCYVEICPGKNTLTARTDKPIQITAARME
                     AFKNRSQIVFSGNATVVQGDSVLKAEKLILHYRQQPKTEKAQKARDNQAGDLEKIEAR
                     GNVSVVQGTRKATADEAVYVQAENKIIMTGNAELKEGKSSIRGERVIILLDEDKGIVE
                     GGERMRVKAVIYPQERRQTENQSTMKN"
     gene            complement(18170..18727)
                     /gene="lptC"
                     /locus_tag="PLR13_04415"
     CDS             complement(18170..18727)
                     /gene="lptC"
                     /locus_tag="PLR13_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQC18610.1"
                     /translation="MIKNRKKTIVFFSVVTVFLAVFLAAFLIFRDSINKPKTLLKALQ
                     DNVDLQIKGFVYTEVGENKGKWEVKAASASYDKKQNLAVLEQVRVTVITADGKKYEMK
                     ADKGRIFTDKKNMNISGNVVITSDDGDNFFTEYVQYSDEERKFFTDAPVRMESRRIRI
                     TGQGLAIFLNSGELSIPSMVKANIH"
     gene            complement(18853..19386)
                     /locus_tag="PLR13_04420"
     CDS             complement(18853..19386)
                     /locus_tag="PLR13_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQC18611.1"
                     /translation="MKKNLKEKLKKIKLLMLDVDGIMTDGRIIMNDEGCETKHFNVRD
                     GHGLKILQRFNIKVAIITGRQSKVVSHRARDLDITDVYQKVYNKKEVFEKILKKYKLS
                     PDETAFMGDDIVDIPVLNRVGFSATVADASDVVKKQVDYITGHKGGKGAVREVCEMIL
                     QAQGKWAEIAARYEFLK"
     gene            complement(19409..20248)
                     /gene="kdsA"
                     /locus_tag="PLR13_04425"
     CDS             complement(19409..20248)
                     /gene="kdsA"
                     /locus_tag="PLR13_04425"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQC18612.1"
                     /translation="MKKIKMGNVTIGDNKKFILIAGPCVLEDEKKAMAIALYLKKLTE
                     RLKMPFIFKASYDKANRTSIKSFRGPGLKKGLAILQRIREKLGVPVLSDVHRFEEIDP
                     ASKVLDVMQVPAFLCRQTDFVTEMAKKARVINVKKGQFLAPWDVANIIEKARAAGNDN
                     IMITERGATFGYNNLVFDVRSLPIMRKMGYPVIFDATHSVQLPGGRGASSGGQRDMVT
                     YLARAAVAAGVDGVFFEVYPDPEKALCDGANSLYLDSLEDLLVTLMKIDALVKTPSNG
                     KNQ"
     gene            complement(20251..22269)
                     /locus_tag="PLR13_04430"
     CDS             complement(20251..22269)
                     /locus_tag="PLR13_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18613.1"
                     /translation="MIHQDRQDRRPKLNGRLPVKTLSIAAAVVVCIAVVALLFYQGFF
                     LSLAFQMSRIKNTFSYYVLKEKPQFYYLDVEKNGQDMRIYRNDVLEITYRDEVVVKAV
                     ASDDLSGKYTYVRFEGLSSRSSDLGVLMKGIDFVNKLIRTGGMSGRSGTVNNYQILVH
                     YLNDTIASVPLKIVITPQDWFRFAKESSNVEQQIDYLKKAIELNQNDVRVRKILAGIY
                     AHHGRTDDAKKLYQQVLTIQSDDTTAMLELVKIHIKQKEFGAAAKLAGDLIKINPKET
                     EGHLALGLALEEKKAWSKAAQHYREAVRLEPHNDAARFRLADAYKNGNMMSNAIDEYQ
                     YIVKHSREPEEALFALGDIYLKLKKTDEAIKCYREILRRQPKNPVAHANLAVAYAGSG
                     KAQEELQSLKKAAALNPNEPTIRFNLGAVYERKKMYGDAAREYRHVLKINPSDTDALE
                     RLADLSLKNKQYSQAVNYYERLKTKSSRKASVYANLGFAYGELKKYQAAASNYEKAIQ
                     LGAKEPVLRANLADTYEKMGMKKKAIAVYEKMPASSKDASTALADLYLKEKNYQKAIA
                     IYQKLAKQEPKKAGSYANLGFAYAAAGNYDQAIKHYNTALKFDSDDADIYDNLGEAYE
                     KKGLYQKALETYKKAHALNPESPKAAQRIPRLNIQLLQERKRQNTGSE"
     gene            complement(22325..23215)
                     /gene="xerD"
                     /locus_tag="PLR13_04435"
     CDS             complement(22325..23215)
                     /gene="xerD"
                     /locus_tag="PLR13_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQC18614.1"
                     /translation="MRALLMKYLDYLFIERGMAQNTLESYSRDLHRYLAFVQTNRKIS
                     DIDNVTPEIVVDYLTKLREEGLAANSMNRALAALRGFYKYLLREKVIAQNPLAHIELA
                     KVWMKLPETISKEEMKTILAQPGNRNNAAIRNTAMLELLYATGIRVSELINLTMNSIN
                     WQVGFLIVMGKGSKERIVPIGRIAYDCVRRYVDEIRPKFMQKKSTDVLFLSRFGKKFT
                     RQGFWKIVVACARQAGLQKKVYPHTFRHSFASHLLEGGADMRTVQVMLGHSDIATTQI
                     YTHITRERLKEVHRKFHPRA"
     gene            23332..23772
                     /locus_tag="PLR13_04440"
     CDS             23332..23772
                     /locus_tag="PLR13_04440"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HQC18615.1"
                     /translation="MKKIVIASDHGAVALKSEIIAFLKTKQCELKDLGVDSDDPVDYP
                     DIAVLTCTEFKRGGYDFGILLCGTGIGVSMTANKIKGIRCALIHDLFTAEMAKAHNDA
                     NFIAFGGRVKYNVPVTKMIEKFMETEFAGERHCRRVGKIMDAEK"
     gene            23805..24665
                     /locus_tag="PLR13_04445"
     CDS             23805..24665
                     /locus_tag="PLR13_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18616.1"
                     /translation="MSDILDKKKIRKVFRNVQKHLSIAQLIRRFSTNKEDIRKAALNG
                     VNLSRCRSVLELGCAFGAFTEALRNKLHDGATITGVDIISEYKPFFLDACRRAGYEGT
                     FLSSGIEKIKKIPSGFFDLVICSYALYFFPEIIPEIARIMKPSGFFITITHSSNDMRE
                     IVDIVKRILGRHHASGTNRSLPIEIIFDQFSAENGESLLQPFFKTIEAIDFKNTLVFQ
                     SQEIRYFMDYFQFKKSFFLSEQDANNQTIIRQLFKELQETLSKNQIVSMCKDDRIFIC
                     SQPSTGKDSP"
     gene            24662..>25204
                     /locus_tag="PLR13_04450"
     CDS             24662..>25204
                     /locus_tag="PLR13_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQC18617.1"
                     /translation="MKKQRKHCLYCGGKTVKKYEDNVRRDFCPVCNSYFYENPLPVVS
                     SILEDSRQILLVKRGKRPSKGLWCLPTGFAEAGESIEEAALRELEEESGIKGKIIKLL
                     DVDSYKSRFYGDLLFLTFVVQQTGGKLCAGDDCSQARFWPVHKLPPLAFRSNKRALDA
                     YIKSRKDYWAIFDSIEHADKK"
     assembly_gap    25205..25304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25305..25658
                     /locus_tag="PLR13_04455"
     CDS             <25305..25658
                     /locus_tag="PLR13_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQC18618.1"
                     /translation="PGFEKDFVESVKEGIMKQEQYDWFCKATNSLYGATPERQLAFAC
                     KNTGFFAMALMLAAKDAGLDSHPMDGFDIDGVHKAFNIPDQYWIPCLLAIGHFDKTKT
                     LLPPKWRKSYEEIVY"
     gene            25726..25977
                     /locus_tag="PLR13_04460"
     CDS             25726..25977
                     /locus_tag="PLR13_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQC18619.1"
                     /translation="MAAKIKIYTLSTCSHCKATKKFLNDNSLPFDCVDVDLLQGDARQ
                     KMLEEVMQYNPDRSFPTILIGDKVIIGFKENAIKEALGI"
     gene            25977..26309
                     /locus_tag="PLR13_04465"
     CDS             25977..26309
                     /locus_tag="PLR13_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HQC18620.1"
                     /translation="MTPAELYDMMKKVQEPKGYFFNKDKDWVLSILGDLLVNKERYGY
                     MCCPCRLAAGNREKDADIICPCQYRPDDVREYGSCFCNLYVSAEWNEGKIEHQYVPER
                     RPKEKIGF"
     gene            26572..26721
                     /locus_tag="PLR13_04470"
                     /pseudo
     CDS             26572..26721
                     /locus_tag="PLR13_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(26957..27721)
                     /locus_tag="PLR13_04475"
     CDS             complement(26957..27721)
                     /locus_tag="PLR13_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQC18621.1"
                     /translation="MTYHSLIVEKKKKYAVIKLNRPHEMNAISKEMHQELYDVFADLE
                     TDSDVRAVVLTGGEYVFSAGMDIKEMNNLPDKESDKFLKSMMMYLKKIYAFKKPVIAA
                     VGGIALGGGFNLVTVCDLVVASESAIFCHPELKFGFNPFFYPLSQIVGITKAKEIVML
                     GEPIGANEAMKLGLVNKVEPPERFMEVAESIADTLSQRSPRAFEELKNLCSIVPRMDK
                     MAAMDIESSICALLFTRDERKEQMREVLSKDKSGRK"
     gene            complement(27718..28002)
                     /locus_tag="PLR13_04480"
     CDS             complement(27718..28002)
                     /locus_tag="PLR13_04480"
                     /inference="COORDINATES: protein motif:HMM:NF001222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HQC18622.1"
                     /translation="MTKNDLIKKLQEELKTYSLKDVTYVVNIIFDSMIDAIKRGERIE
                     LRGFGSFEVRERKPRMGRNPKTGTQVKLTERKVPFFKTGKELRIMVDNKK"
     gene            complement(28016..28912)
                     /gene="sppA"
                     /locus_tag="PLR13_04485"
     CDS             complement(28016..28912)
                     /gene="sppA"
                     /locus_tag="PLR13_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQC18623.1"
                     /translation="MRKHPVLFGMLILFVFGLIFYLSFYNAGFNMGKTSRFSLKNKIG
                     VVPVEGMIADSLEIVEQLDKFGKDRSITAVVLRVDSPGGGVAASQEIYDAILELKKTK
                     KVVVSMGSIAASGGLLIACAADKIVANPGTITGSISAIMQFANFEELLKKVGVKSAVV
                     KSGEYKDIGSPLRDMTPEEKVIVQDLVDDIYNQFVDVIVKERKLSREKVIEIADGRVF
                     SGRKAKELGLIDYLGDMSSAAKLADKLAGGNGEFDLVYPSKKRASIFDYVLEGAASKI
                     SASLKEKMEMNSGLSYLYYPGR"
     gene            complement(28916..30676)
                     /locus_tag="PLR13_04490"
     CDS             complement(28916..30676)
                     /locus_tag="PLR13_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQC18624.1"
                     /translation="MVNNNNSNLSNKNEAGEKSLPKNNITSGKEEMDFKELYEQSLNQ
                     FQYGDIATGKVVQIKDDRIMVDVGWKTEGFIPIDEVRDAQGNVTISIDDEIDVFIDRR
                     DSEGNLVLSRDKASKVKIWDEIKNACENDTVVEGVVVEKVKGGLSVDIGIIAFLPGSQ
                     VDIRPVKDLDKFVGQTLDFKVLKFDRKRNNVVLSRRSIVASEREAEKQDILKNIQEGN
                     VVEGIIKNITDYGVFIDLGGVDGLLHVTDISWGRIVKPSEMFQKGDKITTKVLSFDAE
                     KERVSLGLKQLTANPWEKITETYPVNSIVRGKVVNLTDYGVFVELASGVEGLVHISEM
                     YWTREIKHPSKVLNIGEEIDVKVLEVNAQNKRISLSLKQTTPNPWEKLKEKYPVGTIV
                     KGIVRNITNFGVFVGIEEKIDGLIHVSDISWKHRVNHPSEYFKKGQEVEAVVLNIDVQ
                     NEKFSLGVKQIEKNPWDELAQKYAPGSVVTGKITNFTDFGIFMEIEEGIEGLVHISEI
                     SQKRVKTSSELYSVGDTVSAVVKSIDPKSKKIRLSIKELEAPTPTPSSNQYINNTENV
                     GSNLSQALADVKINTKQKGE"
     gene            complement(30787..31455)
                     /gene="cmk"
                     /locus_tag="PLR13_04495"
     CDS             complement(30787..31455)
                     /gene="cmk"
                     /locus_tag="PLR13_04495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQC18625.1"
                     /translation="MKKKAVITIDGPAGAGKSTISKILAKRLGYIYLDTGALYRGLAY
                     KALKIRISIDSSAELSELCSTTKVDLKNVNGRMRVYVDGEDVENRIRTEEVGLIASKI
                     STFVVVREKLLDLQREAGLRGGLVAEGRDMGSVVFQDADYKFYLDATLAERTKRRHKE
                     LLLKGSTAEYQSIQNDMSIRDKQDQERAIAPLKPPEGSIIVDSDNLSIEEVAEKIMSM
                     ISKK"
     gene            31550..32992
                     /locus_tag="PLR13_04500"
     CDS             31550..32992
                     /locus_tag="PLR13_04500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQC18626.1"
                     /translation="MCKFIQNQYIYLGMIVLMLLLTSDICRAEFTIEDENKLGREFYE
                     QLEQHQMILHNKVLTDYITGIGNLILDQTTRTPFKFNFFILDSSAINAFATPGGYIYI
                     NKGLLLVAENEAQLAGVIAHEIGHANARHVASIISKSQKLNMAALAAMLAGVFLGGGG
                     EASAAITAFSLAGSSSMTLRYMREHEEEADRLGIDYLVHAGYNPSAMVDFLKIMKQYE
                     FLSKTMPSYLQTHPGTDDRIIYMDGLIMTRYRQPGKNSIIGNLCRVQASVPLSASELS
                     RRQRQLETSLAKDPQNTDLLYNLAMIEDQLGANSSALEHLQKALVQSPDDHEILKSIG
                     LLHLKTGRYEQAQNYLLRAKKSDPRNDEITFALGKTYFSSGKYREALDCFLNIRDRAS
                     NEAANLNYFTAMSYGKLQNKGESHYYFGLHFKQANKKDSALFHFREALNFFPPGSERN
                     AEIHRAVKELTAGKDKQKRTIKKDEQNSFL"
     gene            33382..34056
                     /locus_tag="PLR13_04505"
     CDS             33382..34056
                     /locus_tag="PLR13_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQC18627.1"
                     /translation="MNILIFGPNGSGKGTQGAIVQKKYGVPHIETGVIFRQNISKKTP
                     LGEKAKAFIDRGELVPDDITIPMILDRLKEDDCKKGWLLDGFPRNLAQAEALWDALQK
                     ENIKLDSVIEIVLDRQIAKNRIMGRRLCKIDNNHPNNIFIDAIKPHEHDGKTVCRVCG
                     CEDLSTRADDQDTAAIDKRHGIYYDTKTGTLAAVNFYKAKVKVIEVDGSVGVKEVTDE
                     LMKKLG"
     gene            34124..35920
                     /gene="lepA"
                     /locus_tag="PLR13_04510"
     CDS             34124..35920
                     /gene="lepA"
                     /locus_tag="PLR13_04510"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQC18628.1"
                     /translation="MENIRNFSIIAHIDHGKSTLADRLIQFTGVCNERNFQDQILDNM
                     DIERERGITIKSNAISLPYRAKDGKTYILNLIDTPGHVDFSYEVSRSLASCEGVLLLI
                     DAAQGVQAQTLANLYMALEHDLEIIPVINKIDLPSADIDRVLEEIDSELGLDPFTHIK
                     CSAKEGAGIEEVLEAIVLRIPPPKGSPANPLAALIFDAKYDSFRGTIIHCRVFEGTIK
                     SGDIIKFMSNGATYKVEEVGHFLLNRTKREKLSAGDVGYIIAGVKTVSDVRTGDTITL
                     QNRPCVQPLPGFKDVKPVVFASIYPIASDDYQDLADSLEKYKLNDASFIYEKDSSAAL
                     GQGFRCGFLGLLHLDIVQERLEREFNLSIILSVPSVRYRFSLTDKTVIYVDNPAHYPD
                     PIEIDKGEEPYIRSSMMLPERYLGAVMKLCMEKRGVNSVMNYTGPGRVELSYEMPLAE
                     VIYDFYDRFKSVTQGYGSFDYDIIDYRESNLVLMDILVNGEKVDALSQIAHRDRARAR
                     ALLACERLKEEIPRQMFKIAIQGAIGGEIIARTTISAFRKDVTAKCYGGDITRKRKLL
                     EKQKAGKKRMKMIGSVSIPQSAFLAVLKSDTD"
     gene            35959..37086
                     /gene="hemW"
                     /locus_tag="PLR13_04515"
     CDS             35959..37086
                     /gene="hemW"
                     /locus_tag="PLR13_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQC18629.1"
                     /translation="MRSEKAGLYIHVPFCLSKCAYCDFYSVKTLHLIPDYIAAVQKEM
                     KLYRNVFSSFDSIYIGGGTPSLLAPQYLDEIFTALNKYFIIDKNAEITLEANPGDLSP
                     VYLKALRDTGINRLNIGVQSFNDKILKCLGRRHSAQEAMASINAARKAGFRNLGIDLI
                     YGVYGLGLQGWEKILQQALILAPEHFSCYQLSLHEKTPLHKTYLSAGWHLPDDNAELK
                     FFLTTSEILENVGYRHYEVSNFARKDKYTSRHNQKYWQHVPYLGLGPAANSFLNNQRW
                     WNKASVKRYLNAMARGKTPVEASETLTVNQLRLEALFLGLRCKSGIDLKRFKDQYGTD
                     LMTEKKSIMDALMKNKLIELKNGFLCPTRSGMALADSLALI"
     gene            complement(37184..37876)
                     /locus_tag="PLR13_04520"
     CDS             complement(37184..37876)
                     /locus_tag="PLR13_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HQC18630.1"
                     /translation="MMKIGGLQRVSLIDYPGYISAIIFTQGCNFKCPYCHNPELVNPD
                     LFRTPIKVSEVIDFLESRKGKLDAVSICGGEPTIQDNVISFIRQIKKIGFAVKLDTNG
                     SVPNVLKTLIADNMLDFIAMDVKAPLDKYRDIVKANVNQDAIQESIRLVLNAKIPYEF
                     RTTIVESQLDENDILQIGKMINGAGHYVLQNFMAKKTLDKKFAKEKSFSEEKLRKIKK
                     QLEQQIPSVTIR"
     gene            complement(37945..40032)
                     /locus_tag="PLR13_04525"
     CDS             complement(37945..40032)
                     /locus_tag="PLR13_04525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HQC18631.1"
                     /translation="MNLKIRKRDGRLVKFNAEKITNAIAKAGNATGDFDIKEARKLTI
                     KVLNLAEKLFDSKILSVEEIQDVVEEVLLSSPYRKTAKAYIIYRDQHSRLRDIANRME
                     VDLVDQYLKKLDWKINENSNMDYSLQGLNNYISSEVSKVYWLNEIYTPEIHKAHTEGD
                     FHIHDLSLLSVYCVGWDLYDLLLQGFQGVSGKVESKPANHLRSALGQVVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDNLSMKEIKQALQEFIFNINVPTRVGFQTPFTNVTLDV
                     TVPKYYADQNVIIGGKPQKETYREFQEEMNLFNKAFLQVMAEGDAKGRVFTFPIPTYN
                     VTKDFNWDDPNLQDLWEMTAKYGVPYFSNFINSDMNPEDARSMCCRLRIDNRELEMRG
                     GGLFGSNPLTGSIGVITINMPRIAYLSKSKKDFLTRLEHLMELAKESLETKRKVLEKF
                     TYGNLYPYTKYYLRGVKERFGEYWKNHFSTIGLVGMNEACLNLLGVNVATDKGQVFTK
                     QVLDFMRNKLISFQKETGNNYNLESTPAEGTSYRLAKIDKEKFTDIITASEGKSKKAE
                     PFYTNSTQLPVDYTDDIFEALDLQDEIQVKYTGGTVFHIFAGERIDDPQSVKKLVQKI
                     CFNYHLPYITFTPTFSVCPSHGYIKGEQEKCPTCADDCEVYSRVVGYLRPVKQWNKGK
                     QEEFDLRQEFKFK"
     gene            complement(40226..42259)
                     /gene="fusA"
                     /locus_tag="PLR13_04530"
     CDS             complement(40226..42259)
                     /gene="fusA"
                     /locus_tag="PLR13_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQC18632.1"
                     /translation="MASKSKIARTRNIGIVAHIDAGKTTVTERILYYTGKSYKMGEVH
                     DGEAVMDWMPQEQERGITITSAVTTCVWRNHDIHVIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVGGVEPQSETVWHQADKYGVPKIAFINKMDRVGADYFNVIGMMRERFSSLPV
                     AIQIPAGSEDNFRGVLDLINQKMIVWNDSTSGSEMSKQDVPSEFADHVKLQRDKMIET
                     LAEVDDSLADKYLGGEEITIAEIEEALRKATISLKIVPVLCGSALRNKGIQPVLDAII
                     NYLPSPEDIPPVKGMNPVTKEEEIRRSSEKDPLAALAFKIMMDEGRKLTYLRIYSGKI
                     QVNNEIYNPIKKRREKIARLLRMHANKRERIDSASAGDIIAVLGLKSTTTGDTLCAEQ
                     KPILLELIEFYEPVISQAIEAKTPADQEKLTAALDKLVEEDPTLKVKYEDETAQTIIS
                     GMGELHLEIIVDRLLREFNCHVNVGRPRVVYRETIQKKVEIEGIFEKELGEKKHFGHV
                     RIALSPRKRGSGNDVIIKLDDSVIPQDFHEVIEEGIREAMMNGVLNGYPVMDTEVAII
                     GGSFREGESSAQGYKIAASTAFRDGCGQAEPIMLEPIMMVDVITPSEFMGDVIGDINA
                     RKGEIQAVNPKGTVSEIKAKVPLKAMFGYSTDLRSSTQGRAVFSMIFSEYNKI"
     gene            complement(42269..42892)
                     /locus_tag="PLR13_04535"
     CDS             complement(42269..42892)
                     /locus_tag="PLR13_04535"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HQC18633.1"
                     /translation="MSTKKQLRSESIKEKKIKKSTRVFYLSLIVGGGVVLLVIFFITI
                     FSALFPPVDTDVMTKKERRVVKIYFSDQQERFLKAEKRHVIKESDIALQAQEIVKALL
                     DGSKTGLVNTFPKNVSLRDVKIKDHDVAYVNFSKDLIKAYQGSSAAEMNTIYSLTNSI
                     TQNIPEIKRVKILADGKELSSIQGHISTGKFFSPDLELIIPEQSPNN"
BASE COUNT        10945 a        10759 c         9336 g        12062 t
ORIGIN      
        1 cgcgtatccg atttaaaacg cgtgccgcct ttgccgatga agtatcctcc gatcgcaatg
       61 cccattaaaa aacatatccc gacaatcagc gccgctatga ttacaattcg attttcattg
      121 tgcgtcattt tacgataccc tccccgatat tgtattattg taaaaaaaat tcacgatgat
      181 ttttaaaata taagtaacga tagcattata cgttacttaa gaaaaagagc gggaaacaat
      241 acttcatagg ttgtaaggtt ttcttatggc gccatttttc atgaacattt tgccttcctt
      301 aacgcctttt aaaaaaggat ttattttatg atacttttgc gccacacatc gcgcagaact
      361 tagcccccgg ttccagttct gtctgacatt gtttacatct gctttctaaa attggatctt
      421 tgggatgttc gccagacatt gatgccgttt ccactttgct gccgcagacg ccacaaaact
      481 ttgcaccctc ggggataacg gctccacatg cgcatgtatt cgcggacggg acatcttccc
      541 ttaaagagtc ctgagccctc ttctgcacct caacaatctc attttgcaga ttctttatct
      601 gctcatcgat cttcgaaatc tcattgccct tatttttaat gatatctgta tcaatgttgt
      661 tcttcaaaaa cattgcataa gcaatattgc ccaattcctc cagcgcatcc tgcttcttat
      721 tttgaagaga agatatttga tttttcagtt ttgttacatc aagaaactct ttggacttga
      781 cgcttgcctt tgcgacactt ttacttatgc ccagcttgac cttttcaaat aactctgaca
      841 tccgaatctc ctttttttac agacattaat catggatgat aaaattataa attgaatgat
      901 ttcacgtgtc aatgagttta aaagtaacaa attacctcaa aaatattcaa atattcatgg
      961 tgtaatacga tcgtcattcc ccgaaaaact caggccgaac cgacattttt tatgtcttga
     1021 atcttcttga cacacaaaaa catttttttc tataattacg cacagaaaaa acattttcta
     1081 aaagaataaa cgtatgccga atatacagtt ttttgcgtta gccgaaagtg tctgcaaaaa
     1141 gctcaatctc ccgccgattc cttttcctgt ccgcaaaggc cgtgatgaaa taatatttaa
     1201 tccacagggt gtcgccctcg atttgcttct ggatgagctg gagcagtatt taatcgaaaa
     1261 gcccgaagaa aagattttct acaaagatgc attctcgcgg cttgctgcca tcgaaggaat
     1321 ccgtctgggc gaggaaggat tccatgatca cgccgcacac tattttgaat tgggactggc
     1381 ccttgatccg gccaatctag cccttcgatc caattacgca ctttcgctcc aaagtgccgg
     1441 ccgcaaggaa gaggccatgc gtcagtaccg ctttcttctg cagcagccgg taatttccgg
     1501 tcaatgtctt gttttgattc cggccgcccg tctctttctg gattgcggtg atccggtcac
     1561 ggcgcatcag attgtcaagc actgcgcgtc tttcatgccg gtggataacg aattctggga
     1621 tctatttgcc gaagcccgac agcgctgcgg aatcaaacgg tggatggcac cccgccaaaa
     1681 gcaacaagaa attatgcggc aagcaaccgc gcctgattat aaaagtgcgc ccacgggcaa
     1741 gaagaaaaat ttctgcccgg cctgcgggaa tcgcatcaag cccgaagcca gattctgtgg
     1801 aggttgcggt cgtgcgctgt aaaaatgatt tttcggttga atgcatgaag gcgaaggcat
     1861 ttgcagtgca gagcgtgcta tacgcatttc gcgttttcac tccggttctc attttgttaa
     1921 tcatcctatt gctttttgtt tcccatgcta tcgcggacgc ctctgattat cgcgataaag
     1981 gaaaggaaaa ctgggtcctg tttacattta tactcaagga cgccaaaacc ggcgaaccca
     2041 taaaaggccg cgtttattat cagtccgacc tggccgcgga ctggtttcat ccggataaca
     2101 ccaaaccact tttgcctccg ggcatctatc gagtagtggg atgggagaga cattactggc
     2161 aatccgagca aaaactgatt gttaatccac ttaagggatt gcagcagcaa attgttattg
     2221 atctggagcc ggtgtcatct ccaagagacg gcaaatcggg ttcgcccttg aaggttccgc
     2281 cgattttgtc gaacctgatc atcaccggaa acgcagcggt aaaaatgccg catatgaatg
     2341 cgtcatggtt cgggcagagc agtatggcca cttttcatgt gtatacgaat gcgtggtcgg
     2401 aatatcccgg cggcatcggt ggaggctcaa gaaacagcac aggccatatt gattactact
     2461 ttttccttca tacggaagac gctcaaaaac attatgaacg acttcgtccg ggtttcaagg
     2521 taattcccaa cagttcgcaa catgtttctg gcccgactcc ggtttcgctc ggtgatgagg
     2581 cacttcagat cagcatcgac acggagcgtc cggatggaaa gggccttttg tatgaatcac
     2641 gaaagtatct catccgcgat ggtcatttcg tgatgaatat gtacatcaac agagaaatcg
     2701 gcttggattt cctgacctcg cctgaggaaa ttctcaaacg tgtccgtgcg ctcgatgtac
     2761 ggcaggtcaa gcaaatgctg gaacccgagc cttatccctg gcctgaaatt accccaacac
     2821 ccggttcagg aaaagatgtt gattctttag gatctaaaaa tgaggttgcc gattatgcct
     2881 ctgtttcccc ggagaaggcg cttatagctg tggccggcgt gtccatggtg ttgaccttgg
     2941 gggtattggt gcagctttgg ggaatgggtg gccttcagag tccgggagat attcgcggag
     3001 cgctggaggg tcttttcagt tcctccgggg gtgaggtgac agagactgaa tcttctgtac
     3061 ctgagaccgg ggctgtgcag gaagtggagg tcatcccgaa agagccggaa gcactcgtac
     3121 ccgagaccgt tattcaggaa tcccgtgttg tggaaacagt tatccccgat cccgttccgt
     3181 cgggcttcga gtatcagggc aaagtctggt ataagccgcc gtgggacaag ggcggtccat
     3241 actggatgga taaatctgat tacgatgcca tgcgctccat gatgcgccgg ggtatggtct
     3301 ggtcggatcg ctggggctgg gtgactccgg aagacggaaa aaccatggaa gcccagcgcg
     3361 tcgcggcctg ggatgaatat aaatcaaaca cggataaaga catcaaagtc cttaccgaca
     3421 ggattgccga atcgcaggaa aagcttgccg agatccggga aaagcaggcc gaacttgaaa
     3481 gaatcgaaca gttaagagag cgcctgactg agctggaaag acagcgggaa ctggacaact
     3541 catttgccac gcagcttagt gacacctggg acaattatgt cgaaggagtg aacagggatt
     3601 tagacgccct tcccggtgag ttgggcgata tggcactaga tgccgcccgc gggataagag
     3661 acacaaccgg cgcattgatt acagccgccg gggatactgt ttctgattta accaatggag
     3721 acaactggaa agccgtgggc aaggcggcgg tggatacggc caaagatctg attctccatc
     3781 cgttggaatc ttctgttaag gttggtgatg tcgtcaaaga cgccgggagc atagccatca
     3841 aggccgcgga cgttgccgga aatgtggcgg ttgccattgt ttccgatccg tttgcctttg
     3901 cggaatcggt cgtgggcatt gacaactgga aaaaagttct tgatcctaat gtgccagtgg
     3961 gagagcgtgt cagccgcgcg ttatacggag cagtagacac tgctttgaac tttgcctcgg
     4021 gtggaaccaa aacggcggct gccggcgttg aagccggtat ggatgcgctc aaaacagccg
     4081 atacggtatc ggatgcagcc aaagccgcaa atgcagcaga catggcggca gacgcggcac
     4141 gcattacccc gaagctcgat gacccggccc gtgtgcaggt ctgggaagtg ggccgccagg
     4201 caggccagca gaaagcggac aaactggccg aagcgatcaa aagcggcaag ccctctgaaa
     4261 tcaagcaagc tcttgtggaa tgccagaaag acaagcacgc catcggagcg ctcaataggg
     4321 cggatgatac cgtaaaacat gcgtataatc agcaaatgaa acagcttatc acggatgatg
     4381 tggataaggt ggtaaaggaa aaaatcgctc agcgctatgg gctggatccc aaagatgtgg
     4441 agattgtcaa gcccacgaat cccacgtcca aaccgaaggt tggcagcgat cttgatttta
     4501 ccgtcaaagt aaaagcgaaa cccggtgaga tcgtgcccga tcctaataag ccgggaagct
     4561 tcattcatgt gaagccgggg gataatctga ccaaggatgt tcccctcaaa gacgcccagg
     4621 aaatttataa tcgggaactc tacagcaagg taacgggaga gccgattccg acggatcctg
     4681 aaaagtttaa agcctttcag gccgaagccg acaaactcgc gcatgagatg gaccatgtag
     4741 cgggacatcg cgtgggagcg gaatcctacg gcgcctcgca gcaggatttg gaagtcattc
     4801 tgcagaaatc cggcggccgg tttacggatc cggaacaggc gggaatggta gcttcctaca
     4861 agtccacgca tctttaccag gaagcggaaa gcatcacaaa actggatccc ggtaaagccg
     4921 aacagcttgt cgccgacggc atgcggcaga ccactaaaca atacagcaat ctgatcgaaa
     4981 cacgggtggc cgcgctgcag aaccagggca ttgaggttgc cgtggatccg cgcctgacga
     5041 aggcggtgga catttttaaa caggtggaaa ccaaaggcct gcctccggca aaagccgaag
     5101 cgttattgaa aagccagttg aatatgtcca aggacgatgt agccaatatg ctcggcgcgc
     5161 agctggctaa actgcacagg gtgaaagctt aatcatgaag atgagggaat tttaaattat
     5221 gtgggctgtt ttcctgaaaa ttgtttcgta ctttttcctg ctcctgtttg tgctgggcgt
     5281 ggtgttgatg cttttgacct ggcgcaagga aaaattcgtc aagccgaagt ggattctttt
     5341 cgggcagatt gtggcgttta tggcacttgt ggtctttaca atgttgtccc ggaatccact
     5401 gggattctgg agctggttgc tcatctttct ggccgggctg tgtggcggat atttttacgg
     5461 ccgaacggtg aaagtgaaaa agtctgcacg cggaatcatg atgaactaca ccctgcccta
     5521 tgtcatcacg tggggcgtgc ttttattcct cacgcagttt ttgacgatca gcacgggtcg
     5581 tattcctgtg attgtgctgg gcctgtgtgt gctcaatact ggattaaatc tggcgatgaa
     5641 tggccaagtc gtctggaatt ataaccggat gaaaaaaacc tgacccctaa ttacgcatca
     5701 aaagaaggct tttgtttgac tttgtcattg atcagaattg catatgctat ttgccggaag
     5761 tttccctgat cgcttctgta aatccccgca tggaattgac aattgttttg tcatcaacgc
     5821 agtaggcgat cctgaaatat ccggcagcgc cgaaaccgct tcccggaaca accaggatat
     5881 ttttctgcaa aagaatttta gtgaatgcaa catcatccgg tagcgggctt ttgacaaaca
     5941 gataaaaggc cccttgcggt ttggcaaatt tataaccggc ttttgacaac cctttgcaca
     6001 gcagatcgcg cttgttctga tacacgttta catccaccgc tgcattttgc agtttcacga
     6061 caattctctg catcaatgcc ggtgcgtgaa caaatcccag aatccgggtg cataaaatca
     6121 gagcactgat taaatcccgg caatcgtcaa tttccggatt gacggcaata tagccgattc
     6181 tttcccccgg cagagaaagc gtcttggaat aggaacaggc aacgatactg ttcgtgtagg
     6241 atttcagcac gctggggact attacccggt cataaacgat ttcattatag ggttcgtcgg
     6301 aaaccaggta aatggtttta tgaaacgttt tgcccttttt cttcaaaagg gcggcaagcg
     6361 cctgaatgtt ttttgaactg taaacctttc ccgtcgggtt attcggtgaa ttgattaaca
     6421 ccgcttttgt ctttttgttg atggcctttg agatcgcctc gatgtccaga gaaaaatctt
     6481 ttctggttct gacgaatttt gttacgcctc cgtagttgtc gatgtaaaat ggatattcga
     6541 cgaaataagg catggggacg atgacttcat cattgggatc cagtaatgat ttgaaaataa
     6601 cattcaacgc accggctgcg ccgcaggaca tgacaatatg gtcggcgctt aaattcagat
     6661 cgcaggtttt actgacctgc ttggcaatag cctgccgtgt atccgcaaaa ccggcgttcg
     6721 gcgcgtatcc gtgcttgagg ggcaatggct ttgcggccag acgaatcaat tccctcttga
     6781 agactgccgg aggctcgacg ttgggattcc ccaggctgaa atcaaagact ttatcggcgc
     6841 cgtattttct tttcatcacg gcgccttctt caaacatctt tctaatcatc gaagaattcg
     6901 aaatcgatga tttaattttt tttgcaattg acaacgaatg tattatcctt tctttcttct
     6961 gaggagcaca aaaatgccca gcaaaatgat aataccgggc aacacatatt tcgaatactg
     7021 ggaaacatcg aatccgtaga actcataaaa gagaaacccc gtcccggcaa gcgttataat
     7081 ccatccgccg agatctctga acttttgtat tagcgtgcag ataccgatga caatcaacaa
     7141 aaccggccag gtctggccga acgggaaact tccggacttg ctcagataaa tcaaaattcc
     7201 caacgtaatt aaaaccgttc cgcccgtaat cacattctgt ttcttatcgc tcatgcaaga
     7261 atctcctttt cgtgtattgg ttatttaaag cgtctctgct tttttccgga aattatttat
     7321 ctttcaaaaa aggtttaaac aaatcaatcg gtatcggaaa taccgttgtg gaattatttt
     7381 ccgaagctat ctgattcaat gtctgcaaat agcgaagctg taaggacatt ggctgggttt
     7441 ccatcaaagc agccgcttcg atcagcttct gcgctgcttg aaattcgcct tcggcattga
     7501 tgaccttggc tcttcgttct ctttccgctt ccgcctgctt tgccatagac cgtttcatct
     7561 cttccgggag gtcaatatgt ttgacttcta ccagcgacac cttaatgccc cagggttcag
     7621 tgctgcggtc gagaatttcc tgaagattca tattaatttt ttcccgttcg gaaagtattt
     7681 catcgagttc catctggccg cagacacttc tcaaggtggt ttgagcaagc tgcgatgtgg
     7741 cgtaaagata attttccaca ttcgtcacgg cggaattggc atccatcacc cggaaataaa
     7801 caaccgcact gactttaatg gaaacgttgt ccttcgtaat aacgtcctgc ggaggaacat
     7861 ccatggtaat ggtccgcata tccacccgga ccattttgtc aatgccggga ataatgataa
     7921 tcaagcccgg ccccttggca tcccggattc gaccgagtcg gaaaataacc gccctttcgt
     7981 attcgctcaa tattttgatt gcggagacca aaaacattac gaccagaacg atcacgacca
     8041 gcgtcggcat agataaagtt cccaacatat gtattcctcc tctttttatt ctaatcctta
     8101 tttttgattt tttatttctt caacgaccag tttcatgccg tcaaccttga cgattctcac
     8161 cttggcgcct tttttgatgg acgcgtcact gacggctttc cagtattctc ctttgacaaa
     8221 aacatcgccc tctttttcga tgtcgctggt tacttcggct tccagatcaa tcatggcttc
     8281 cgcgccggta aaatgttttc tcatctgcgc cttcactgcc agcataatga tgacgataaa
     8341 gaaaatagag acaaccacaa cggccggtat caatacctgc aaggaaagac gcaaggccgg
     8401 ctccggcgta tcgaacagca ataacgatcc cataaccaat gaaataatcc ctcctaccgt
     8461 cagcatgccg tgactcatga ctttaatttc cgcgataaat aaaatgacgc cgaatattat
     8521 cagcaaaatt cccgtataac ttaccggcag cgtggacaag ccgaaaaacg caagcaaaag
     8581 cgaaatgccg ccgatcacgc ccggcagaat cgcgccggga gtggacagtt cgaagtaaag
     8641 ccccgccagt ccgatcagta atagaagata tgatatattg ggatccgaaa tggcggaaag
     8701 aataccctga cgcgtcccca tttttttatc gcgaatcacc gcgttttttg ttgatattat
     8761 ttttttgccg ccgatcaact gaatctcttt ctggtggatg gcagccagta acatgttaac
     8821 atccgccgca acataatcga tgacattcag cttcaaggct tcctccgccg tgatcgattc
     8881 gcttttacgc accgcttttt cgacccattc ttccgaccgg cctcttgttt tggcaatgct
     8941 tctggcatag gcggcggcat cattttcaat tttttccgtc atggttttat ctttttctcc
     9001 gcccgaattc ccgagaccga ttgccacagg atgcgcggcg ccgatattgg ttcccggcgc
     9061 catcgctgca atgtgagccg cctgggtgat gatgacgccc gcagaagcgg cccgcgctcc
     9121 ggacggcgat acataaacga tcacgggtac cggtgaattc agaatgctct tggcgatatc
     9181 gcgcatcgcc gtatccatgc cccccggcgt atccagcaga atgatgagcc ccccggcatt
     9241 atcccgaacg gcttcctcga tgcctgatgc aatgtatttc gcggtcggtg acgtaatgac
     9301 accatccacc gtgataacat tgaaaatagg atttttttcc tctgcccacg ccagaagtcc
     9361 tgcgaaaatg aaaacagcca ttaatgcata tcggatttgc ttcattgcct tacctcgttt
     9421 gatggctcaa tacggaaggt ttaattcctt catgatgatt tttacatctt cccatacctg
     9481 cttcttcgcc gccggattgc gcagaagata ggccggatga taggtcggca ttaattttat
     9541 tccttcatag gaatgaaacc ttccacgcag cactgaaatg ggaacatctg tttttagcag
     9601 cgtcttggcg gcaaacgtcc ccaatgcaca aatcacttcc ggtgctatca actggagttg
     9661 tttcttgaga aaaggttcgc acctgctgat ctcatccggc tgcgggttgc ggtttcccgg
     9721 aggacggcat ttgaggatat tgcagatata aacatccttt ctctgaagct tcatgccttt
     9781 aacaataatt tcggtcaaaa gctggcctgc tctgcccaca aaagggatgc cctgctcatc
     9841 ttcatcagcg ccgggagctt caccgacaaa aacgatccgc gctccgggat tgcctgttcc
     9901 aaaaacaaga tttttccggg ttctgcccag cggacagagc tgacaatcgg ccatctcttg
     9961 gtgtatggct tccagcattt cccctctgga ttgccctccg gtcttggctt tagcaacaac
    10021 ggtccgtgat gatctgctcc aatccacgcc aaccgccaga ctctcgttat ggatgacctg
    10081 tcctttcagc cccctgaccg catggagtaa ttcggcttgt aattcgtttc tggttatgtt
    10141 cgacatcgtt cgcctgttat ttcttgcctt tgcttttttc agaaatcaat tttttaatac
    10201 gattcagaat cgcaccggcc gcatcgattt ttgtcatttt ctccagcgac tccgtcttac
    10261 cggcgcggtc gatgacggta ataatgttgg tgtcggtgcg gaaaccggca ccctcctgac
    10321 gcagattatt ggcaacgatc atgtccatgt ttttcttgac cagttttccc cgggcattgg
    10381 ttaaaagatt ctgcgtttcc atggcaaaac cgaccagaat ccggtttccc ttttttttgc
    10441 cgatttccgc gatgatatct ggatttcttt ccagttctat aaatagcgtt ttcccttcct
    10501 tttttatttt ttcattggcc acgattttcg gacgataatc ggctacggcg gcggccttga
    10561 taatcaccct tgcactttta taatgctgca gaacagcttt gtgcatttct ctggccgtac
    10621 gcaccgtgac gattttttcc aacggcggca gcggcagtga cgtggggccg ctgaccagcg
    10681 tcacgcgggc tccccgtctt tgcgcagcgg aggccagagc gtaacccatt tttcccgacg
    10741 acaaattcgt gatgaaacgc accgggtcga gcggctcttc tgtaggaccg gcagtgatca
    10801 gaattctgac gcccgccaag tcctgcggcg tcataatgtt ttcgatttca gcaaggatcg
    10861 cgtcaatctc cggcaagcgt cccttgccct ctgttttaca ggccagttcg cctacggcgc
    10921 tgtccatgac tgcgtaaccg ttttttttca gtctccggat attgtcctga acaatcggat
    10981 tggccagcat tttgtcgttc atcgccggac aaatcagcgt cggtttgttc tcggccatga
    11041 tcgtggtgga aagcaaatcg tcgccgatgc cggaagcaat ttttccgata atgtttgccg
    11101 ttgccggagc aaccacaaac gcgtcggcaa attcggccag cgcgatatgt gccatgtcat
    11161 attcgtcatc agaggcaaac atatccgtgt aaactctgtt ttgcgaaagc gtctgcatgg
    11221 taagaggcgt aatgaattcc agagcgcttt ttgtcatgat gatttttaca tgggcacctg
    11281 ctttcacaag ccggcgggtc aattccgccg ctttgtaagc cgcaatgcct ccggtgattc
    11341 caagaacaat tttcttattt tccagcatat ggttcgactt ccagaaattt ttgattaatt
    11401 tttttgaaaa cagctcgtgg cgcttggccg ttgtctatga atataacaat gctttttcac
    11461 aatatcaagg tataattatt attgatatct tgaaaatttt ataaggaaat ggtcccatgt
    11521 agcttaattc ttcactgtgc ttgcgccgct ccgcttcgct gcggggataa ttccacttac
    11581 gcttcgagct tcattacatt cgctcttagc acgtggcatt aatttccctt gcaaatcctc
    11641 cggaatgcta ttataaaccg agcaattcta tttacatcac aacgttagga gattattatg
    11701 aaatggttcc gttttgatat cagaattctc atcgcgctcg tggtcgtcgc cgccctgggc
    11761 tggtttatct tcggtaatta tatcgaaata ggaaatcctg tgattaaatt agacagtgat
    11821 gtgaaagcca tcggcagaca gaaaactgtc ggtattacgt tttccgattc caaaagcggt
    11881 ctttcccgga tcacggcgga gatcattcag gacaacaaag gtcacgttct ggccgataaa
    11941 aaaatcccgc aaaagggcgt ggcccatgaa tccctgagtc tgaatatcgc cagcactcaa
    12001 ctggcgctcc atgacggccc cgcggttttg aaaatatcgg cgacggatca ttcgcttttg
    12061 aagaatcaga cggtgctgtc gcagtccgtc acaatcgata cgattccacc gatgatcaac
    12121 cttctaaaga atatcaatta catcaatcag ggaggcacgg gctttgtcgc gtatcagcct
    12181 tcaaaacccc tctcatcgac gggcgtttat atcaatgatt acttgtcgcc ggcgcatgcc
    12241 gtcttgatcg acaataaacc ttcgtacatc acgtatttcg cgctgccgat gactgccgat
    12301 aaagcagcta caagcattat gatctttgcc cgggacgaag ccggcaatga aacccgtgtg
    12361 tccgttccct gcgtgattaa ggaaaagaaa ttccgttccg acaaaatgca tctttcggaa
    12421 gcgttcctcc agcgtaaaat gccggaattc caggccatgg ttccttcgct tcagggcaaa
    12481 acgcttctgg atgtattcac ctatgtcaat tcccagatgc gaaaagaaaa cttttccgcc
    12541 atacagaaaa tctgcatgaa atcggctccc aaagaactct gggaaggcga attcctgcgc
    12601 atgagcaatg ccgctcctat ggccctgttc ggcgataagc gcagttatgc gtatgaagga
    12661 aaagtggtgg gtgaatccgt tcatgaaggc gtggatcttg ctgccaacgc gcgcagcccc
    12721 attgaggcat ccaataaagg gattgttgtc ttcaccggag ctctgggcat ttacggcaat
    12781 acggttatta tcgaccacgg tctgggatta ttcagtcttt acgcccatct ctcatccatc
    12841 gacacgaccg tgggcaaaac cgtggcaaaa ggcgaaaaaa ttggtctttc cggcatgtcc
    12901 ggactggcgg ggggagatca tctgcacttc agtattatgg tcggcggaaa atttgtcgac
    12961 cccaaagaat ggtgggatcc ccactggatc aaagacaaca tcacccggta aagcagcgtt
    13021 tcaaccggcc atcggctttt aatgtttatt gacatcccgg tgattgacat tgacatggta
    13081 cttcagcgag gtaaaaaaag cgctcatttt attggccatc acaactgatc gatgcttccc
    13141 gcccgtacaa ccgatggcaa tattgaggag caccttccct tctttttcat aaagcggggt
    13201 caagagattc attaaatctt tgattttttc cagaaactgt ttgctttccc tgttgcgcaa
    13261 gacgtagttt tgcacgttcc tgttattacc gtcaaaattt ttcaactttt ctataaaata
    13321 agggttgggt aaaaatcgga catcgaaaac aaggtcggca tccaccggca atccataacg
    13381 gtaaccgaac gactggacat tgattaccat tctcttgttt tttgaaaata ccgaaaaaga
    13441 cctctgaaca acgtccttta attgatgcac attaaaggac gttgtatcga tcaccttatc
    13501 cgccattttc ttcagggaag ataacttttc gcgttccagc gaaattcctt ccataataat
    13561 gccccgttcc aggagagggt ggacacgtct ggtctcgctg aagcggtgga caagagactc
    13621 gtcgctggac tcgagaaaaa gaatctcgat tttgtatcct tttttcttca ggctcgcaaa
    13681 aatccggctg tatttatcca gaaagcttcg ttgccttaaa tccataacca gcgccacgcg
    13741 cttgatttcc ggtgaagaag ccagcgtgat ggcaagaaat ttcggcagta aaataacggg
    13801 aaggttgtcg acgcagaaga atccgacatc ctccagcgcg cgcaatgccg tacttttacc
    13861 cgacccggaa atgcccgtta taataacaac ccgcaaattt ttcattttat ttcccctgga
    13921 tgtgccgaat ttttagcaca cattttataa attttacaac aataaaaagc cgcaggcccc
    13981 caacggattc ctgcggcaaa agaaaaaacg ttttgatgtc ttgtcccgtt cagacaatac
    14041 aggtgcgttc ctgatcgatg ataatctgat acaattcatc ccgggatttt gccgcgatga
    14101 gtttgttacg gaagtaaacg tctttgagca ttttggatat tttggccaga attttcaggt
    14161 gctggccggc agagttttcc ggcgcaagca aaagaaaaaa gagatgcacc ggtttgccgt
    14221 cgagcgaatc ataagcaatg cccttttcgc tgcgtccgaa agcaacaaca atgtcattca
    14281 gattggatat ctttccgtga ggaatggcaa ccccctctcc tatgcccgtg gaaccgagat
    14341 tttcacgctg ctttaaaata tcaatgattg aagaactgtt cagttttaaa ccgccttcaa
    14401 tgataacatt cacgagttcc gcaaggactt catccttgtt tttcgcctct aaaccggcat
    14461 taataaattc cattttaaaa atctgattca gctgcattgt tcaaccatat cttccatgtc
    14521 tattgacttg cttgatgatc acctctagaa aagctccgac gctttcattt cgtttcgata
    14581 agagtgtaac ggccgtcatc ccctctgtag atcatactga cattttcaga cgacgtatcg
    14641 cgatacagca caaaacggtc atttgtttcc tcgatttcca tgatggcttc atcaaacgac
    14701 atgggcttta agataacttt tcgggtttcc atgattttac ttgccgtcgg ttcctcgatt
    14761 tcttcctcgt tgagaggttt ttcctgagca cgtctgatcg tcttcggttt gtgttgtctt
    14821 attttctctc tctgtttctt gagctgtttt tcgatttttt caacacaccg gtccagagca
    14881 acgcgcatat ccttggcttc ttctttcgcg ttgatgttcc atccgttgga gttcaaatta
    14941 atttccgtca catttctgaa cttttccata gagacaacta catgagcttc ggcgggcgca
    15001 tccagatatt ttttcagttt ctgcattttt tcttcggcgt aatttttttg ccagttctcc
    15061 ccttccccgt tcctgaacgt taccgaaatc ttcatcattg ctcctcctta cataattaaa
    15121 ttcatcgtcc tgataacctt catcgaccga ttgactttct ttgcgccggc aatcaaaggt
    15181 gcgaacctct ttgccaaacc gctgcataga attgaagcct cggctgcgcc gtgtttttca
    15241 acctgccaat cccgatgctt cgcttcggca ccagagtttc accataaagg gacccgtcat
    15301 atattgggct caaaactaaa agtcaaccaa aatcttacac cggttgtgtc tttgtccgtc
    15361 aaatcgcttt aaaatatttc tttcgcttcg atgaaggaag aatccccatc atttcccggt
    15421 acttggccac cgttcggcgg gcgatatgaa ttccctttgc cttcagtaat tgaaccattt
    15481 cgcagtcgct gtagggttta cgcggcggtt ccgagctgat cagttgccga atttcttcct
    15541 gcacgctttt cgatgcaatc gttccctgtg ttgtcgttct gatcgaactt ccgaagaaat
    15601 acttcaattc gaaaattccc tgcggcgaat gcatgtattt attattaacc acgcggctga
    15661 tggtggattc atgcatctcg atatcattgg caacatcccg taaaatcaaa ggtctgagat
    15721 aatcgatgcc tttatcgaaa aattcttttt gaaattttac aatactttcc gccaccttat
    15781 atatggtgtt ttgacgctgt tgaatgctct tgatcaacca ggttgccgat tgtaccttgt
    15841 ccttgatgta ttttttcccg ttttcggcct cttcatgact atgactcgaa atgccggcca
    15901 ttacttcccg gtaaaaatta ctgatgcgca gtcgcggcaa gccatcatcg ttcaaaataa
    15961 ttttatattc gtctcctgtt ttgacaacat agacatcggg aatgatcggc tgcgctttgt
    16021 cttccgaata aatgctcccc ggcttcggat tcatttcgct gatcagcaat accgccattt
    16081 ccacttcctt gagcggaact tttaatttat gggcgatttg agcatagttt ttcagttcca
    16141 gctctttgag aaaatctctg attatcactt caataatccg gttttttacg cccagcatcc
    16201 tggcctgtat caacaggcat tcctgaagat tacgcgcggc aattcccgga ggatcaaatt
    16261 cctgaacttt ttgaagcact ctttccacca gctcttcact gacattttcc agttgtgcaa
    16321 tttcctgagc cgttgcgcat aaatatccat tgggatccag attgccgata atctggctgc
    16381 cgacccgttc ttcgtcttcc gaaaaggacg acagtttcat ctgccaggtc agatgctttg
    16441 ataacgtctg accttctgtc agcacattat cccaggatgt ttcctcccca tccttgccgc
    16501 cgtacgtcat gcccaccggg ccgtaatctt ccaggtagct cgcccagtcg aattcgtccg
    16561 ttccggctcc tttttcaaca ttcaattctt ccgtatgttc gaccgctttt atcgtatcgc
    16621 gttcgacggg caggccatca ttttcaaatt ccccgatcaa tgcctcgtcc gaattttcat
    16681 cagtggaaat ttcctccagc aggggatttt cttccatctc ctgattgatc gcttccacaa
    16741 gttcctgacg ggacagctgc agcagcttga tggcctgctg gagctgaggg gtcatgatca
    16801 gctgctggga aagttttaga tgctgtttta actcaaatgc cataatcgga attcagtaca
    16861 agcctgttat aaattaaatg aatctccaaa ataaaatttt cgggccagtt cgctctgcgc
    16921 aatggtgtcc ggcggtcctt ccaccaggat ctctccattg ttgacgatat acgcccggtc
    16981 acagcaggaa agcgtttccc gcacattatg atcggagata atgatgccga tgcccttggc
    17041 tttcagtttt tcaataattt tctgaatatc ggcaacggcc agcggatcaa tgcccgcaaa
    17101 cggctcatcc agaagaatat atttgggaga ggtcaccaga gcccgtgtaa tttcgacccg
    17161 ccgtctttct ccaccggaaa gagaataggc ataatttttt gccagtgcgg tcaggtccag
    17221 ctcctttaaa agctcgcgaa gccgctcctg tctttccgct tcactcaaat caagagtttc
    17281 cagaatcgcc aggatgtttt cctccacagt cagcttccgg aaaatcgacg gttcttgagg
    17341 cagataattc aggcctttgc gtgctcgccg gtacatggga tttcccgtaa tgttttcccc
    17401 gtcgagataa atctctcccg catccggctt gaccaatccc acaatcatgt aaaacgtcgt
    17461 cgtttttccg gcgccattgg gccccagcag accgaccact tctcccggtg caatcaataa
    17521 gtcgatctga ttgaccactt ttctcttgtt ataaattttc gttaaacctt tagccgataa
    17581 atcgctcatt ttaaatcatc agtttttcat ggttgactgg ttttctgtct gtctgcgctc
    17641 ctgcggatag ataaccgcct tgacgcgcat gcgctctccg ccttccacaa tacctttatc
    17701 ttcatcaagc agaataatca cccgctcgcc tctgatcgaa ctttttcctt ccttcagttc
    17761 cgcattcccc gtcatgataa ttttattttc cgcctgaaca tatacggcct catcggccgt
    17821 tgccttccgc gttccctgca ccacggaaac atttccccgg gcctcgattt tttccagatc
    17881 cccggcttga ttgtcgcgcg ccttttgcgc cttctctgtt ttcggctgtt gccgataatg
    17941 caaaataagt ttttccgcct ttaatacact gtcgccctga acgaccgtgg catttcccga
    18001 aaaaacgatc tggctccgat tcttaaacgc ctccattctt gcggctgtaa tctgaatcgg
    18061 cttgtccgtg cgggcggtca atgtattttt acccgggcag atttcaacgt agcaaaagaa
    18121 aaaaaacagc attaaaaaaa tgtatgtgtg tttacggatc attcttttct caatgaatgt
    18181 ttgccttgac catcgacgga atactcaatt ccccgctgtt caaaaaaatg gccaatccct
    18241 gcccggttat tctgatgcgt ctgctttcca ttctgaccgg cgcgtccgtg aaaaattttc
    18301 tttcctcatc gctgtattgg acatactccg tgaaaaaatt atcgccgtca tccgacgtga
    18361 tcacgacatt gccgctgatg ttcatgttct tcttatccgt aaaaatccgg cccttatccg
    18421 ccttcatttc atacttttta ccatccgccg ttatcaccgt tacccggacc tgctccaaaa
    18481 cagcaagatt ttgtttttta tcgtatgacg ccgatgccgc ttttacctcc cattttccct
    18541 tattctctcc aacttccgta tagacaaacc ctttaatttg cagatcaacg ttatcctgca
    18601 gcgccttcaa taacgttttc ggcttattga tgctatccct gaagattaaa aaagctgcca
    18661 gaaaaaccgc cagaaaaacc gttacaacgg agaagaaaac tattgttttt tttctgtttt
    18721 ttatcacaat ccgaaacatc ccgcagaaac tttttttatt tgtttttatt atatcactca
    18781 gaatgcaggg tgtaaagcta taaggggtgt actgcgttat aaaaaccgtt taaaaaaagc
    18841 catgacgaaa tgttatttta aaaattcata tcgcgcggca atctccgccc actttccctg
    18901 agcctgcaga atcatctcac agacttcccg tacggcgccc tttccgcctt tatgtccggt
    18961 tatataatca acctgctttt tgaccacgtc ggatgcgtcg gcaacggtcg ctgaaaaacc
    19021 gaccctgttc aacaccggga tatccacaat gtcgtccccc ataaaagccg tttcgtctgg
    19081 cgagagcttg tattttttta gaattttctc aaagacttct ttcttgttgt atactttctg
    19141 atagacatct gtaatgtcca gatctcttgc ccgatgcgag acaactttgg attgacgacc
    19201 ggtaataata gccactttga tattaaagcg ctgcaggatt ttcaatccat ggccatcgcg
    19261 gacattgaaa tgtttcgttt cacacccttc atcattcata ataatccgcc catccgtcat
    19321 gatgccatcc acatccagca tcagcagctt tatttttttg agtttttcct tgagattctt
    19381 tttcattttt tattcagcct tttttctttt attgattttt cccgtttgac ggcgttttga
    19441 ctaaagcgtc gattttcatc agagtgacaa gaaggtcttc cagcgaatcc agatagagtg
    19501 aatttgcacc gtcgcacagc gctttctcgg gatcgggata gacttcaaaa aagacaccat
    19561 caacaccggc agccacggca gcgcgcgcaa gatacgtcac catgtctctc tgtcctcccg
    19621 aagacgcccc ccggcctccg ggcaactgca ccgaatgcgt agcatcaaaa atgaccggat
    19681 accccatctt acgcatgatc ggcagggaac gcacatcaaa aacaagatta ttataaccga
    19741 acgttgcgcc cctttccgta atcatgatgt tgtcattgcc tgccgcacga gccttttcga
    19801 tgatattggc cacatcccac ggtgccagaa actgtccttt tttgacattg atcacacgcg
    19861 ccttttttgc catttcggtg acaaaatccg tttgacggca taaaaatgcc ggcacctgca
    19921 tgacgtccag aactttcgag gccggatcga tttcttcgaa acgatgaacg tcggacagta
    19981 ccggcacacc cagtttttcc ctgatgcgct gcaatatcgc cagacctttt ttcaatccgg
    20041 ggccccggaa ggatttaatc gacgtgcggt tggccttgtc gtaagacgct ttgaagataa
    20101 aaggcatttt cagcctctcc gtgagttttt tcaaataaag cgcaatcgcc atcgcctttt
    20161 tctcatcttc cagaacgcag ggtccggcaa tcaatataaa ttttttatta tctccgattg
    20221 tgacattacc catttttatt tttttcattt ttattcactc cctgtatttt gccgctttct
    20281 ctcctgtaat aattgaatat tcaatcgggg gatccgttga gcggcttttg gcgattccgg
    20341 attgagtgca tgagcttttt tataggtttc cagggctttc tgatatagcc cttttttctc
    20401 atacgcttct ccgagattat cataaatatc ggcatcgtcg ctatcgaatt tcaaggccgt
    20461 attataatgt ttgatggcct gatcgtaatt accggcagcc gcataggcga acccgagatt
    20521 ggcgtaagat cccgcctttt taggttcctg tttggccagc ttctgataga ttgcgatcgc
    20581 cttctgataa tttttttcct tcaggtaaag atctgccagc gcggtcgacg cgtcttttga
    20641 cgacgcaggc atcttctcgt acacggcaat cgccttcttt ttcatgccca tcttttcgta
    20701 ggtatccgcg agattggcac gcaaaaccgg ttccttcgcg cccagttgaa tggccttttc
    20761 gtaattcgaa gccgcagcct ggtatttttt taattctcca taggcaaaac cgagattcgc
    20821 ataaaccgag gccttgcgcg atgattttgt tttcagcctc tcataatagt tgaccgcctg
    20881 gctgtactgt ttgtttttta gtgacagatc cgccagcctc tccagggcat cggtatcgct
    20941 gggattgatc ttcagaacat ggcgatattc tctcgccgca tcgccataca ttttttttct
    21001 ttcgtaaacg gcaccgagat tgaaccggat ggtaggctcg ttgggattaa gcgcggccgc
    21061 ctttttcaga ctttgcagtt cctcctgcgc cttgccgctt ccggcatatg ccaccgccag
    21121 attcgcatgg gctaccggat ttttaggctg tcgtctcaaa atttcccgat aacatttaat
    21181 cgcctcatcg gtttttttga gtttcagata aatatcaccg agcgcaaaca gcgcctcctc
    21241 cggctcccgg gaatgtttga cgatgtattg gtattcgtca atggcgttgc tcatcatatt
    21301 gccgtttttg tatgcgtccg ccagtctgaa tctcgccgcg tcgttatgcg gttccagccg
    21361 gacggcttcc cggtaatgtt gcgccgcttt ggaccatgct tttttttcct cgagagcaag
    21421 tcccagcgcc agatggccct ccgtttcttt gggattgatt ttaatgaggt cgccggcgag
    21481 cttcgctgca gcgccgaatt ccttttgttt gatatgaatt ttcaccagct ccagcatggc
    21541 ggttgtgtca tcggattgga tcgtcaggac ctgctgatac agttttttcg cgtcatccgt
    21601 tcttccgtga tgcgcataaa taccggccag aatcttgcgc acacggacat cgttttgatt
    21661 gagttcgatg gctttcttta aatagtcaat ctgctgttcg acgttggaag attctttcgc
    21721 aaacctaaac cagtcttgag gtgtaatgac aattttcagc ggcacggacg cgatcgtgtc
    21781 attcagataa tgcacaagga tttgataatt gttcaccgta ccggaacgac cgctcattcc
    21841 tcccgtcctg atcagcttat tgacaaaatc aatacccttc atcaaaacac cgaggtcgct
    21901 gcttctgctg ctgagccctt caaaccggac gtaggtatac ttgccggata aatcatcact
    21961 ggccaccgct ttgacgacaa cctcatcgcg gtatgtaatt tccagcacgt cattccggta
    22021 tatgcgcatg tcctgaccgt ttttttccac gtccaaataa tagaattgcg gcttctcctt
    22081 cagaacataa tacgaaaagg tatttttgat cctggacatc tgaaaagcca gcgacaaaaa
    22141 aaatccctga taaaaaagaa gcgccacaac ggcgatacaa acaacaacgg cggctgcaat
    22201 ggatagcgtt tttaccggca accttccatt taattttggt cttctatcct gtctatcctg
    22261 atggatcatc gcatttctcg tttctgcatg acgatgcttt tgataacggc tttcctgttg
    22321 ttcttcaagc ccgcggatga aatttgcggt gaacttcctt caaccgttcc ctcgtaatgt
    22381 gggtatagat ttgcgtggtt gcgatatccg aatgtcccag cataacctgc acggtacgca
    22441 tatcggcgcc gccttccaga agatgcgacg caaaggaatg cctgaacgta tgcggataaa
    22501 ccttcttctg caatcccgcc tgacgcgcac aggcaaccac gatcttccaa aacccctgcc
    22561 tggtgaattt ttttccgaac cggctcaaaa acagaacatc cgtgcttttt ttctgcatga
    22621 atttcggccg gatttcatcc acataacgcc tgacacaatc ataagctatt ctgccgatcg
    22681 ggacaattct ttctttactg cctttcccca tgacaatcag aaatcccacc tgccagttga
    22741 tgctgttcat cgtcaaatta atgagttccg acacacggat gcccgtcgcg tataaaagtt
    22801 ccagcatggc ggtattgcga atggcagcat tattccggtt tccgggctga gccagaatgg
    22861 ttttcatttc ttccttgctg atcgtttccg gaagcttcat ccagactttc gccagctcga
    22921 tatgggccag cggattttgg gcaataacct tctccctcag cagatatttg taaaatcccc
    22981 tcaaagcggc cagcgctctg ttcatggaat tcgccgccaa accttcttct ctaagctttg
    23041 tcagataatc cacgacgatt tccggcgtta cgttgtctat atcgcttatt ttccggttgg
    23101 tctgcacaaa ggccagatac cggtgcagat cccggctgta ggattccagc gtgttctgag
    23161 ccattccgcg ttctatgaac agataatcca gatatttcat taataatgcc cgcatatgac
    23221 tttttaactt aattatcttt ctgaagcaaa gattttttat tgacttattt ctgaatatca
    23281 taaaaatagg aacagaaacc tctctaaaac gttactgaca aggaaccgct catgaaaaaa
    23341 attgttatcg cttccgatca cggcgccgta gctttaaaat ccgaaatcat cgcttttttg
    23401 aaaacaaaac agtgcgagtt gaaggatctc ggagtcgaca gcgatgatcc ggtcgattat
    23461 cccgatatcg ccgtgctgac ctgtacggaa ttcaaacgag gcggatatga tttcggcatt
    23521 cttttgtgcg ggacgggaat cggcgtatcc atgaccgcga ataaaatcaa aggaatccgt
    23581 tgcgctctga ttcacgacct ctttaccgca gaaatggcca aagcccataa cgacgccaac
    23641 tttatcgctt tcggcggccg ggtaaaatat aatgtgcccg tcacgaaaat gattgaaaaa
    23701 ttcatggaaa ccgaatttgc cggggaaagg cactgccgga gagtcggtaa aatcatggac
    23761 gctgaaaaat aatattcaga cagctttttc attcaggaat ccaaatgtcg gatattcttg
    23821 ataaaaaaaa gattcgcaag gtgtttcgca acgttcagaa acacctgagc attgcccagc
    23881 ttatccgccg tttttccacc aacaaagaag atatccgaaa agcagccctg aacggggtca
    23941 acctgtcccg atgcaggagt gttttggaac tgggctgcgc gttcggtgct tttaccgagg
    24001 cgctgagaaa caaactgcat gacggcgcaa ccattacagg cgttgatatc atctccgaat
    24061 acaagccttt ttttctcgat gcctgccgac gggccggtta cgaaggaacc tttctatctt
    24121 ccggaatcga aaaaataaaa aaaatcccat ccggattctt tgatctggtg atctgcagtt
    24181 acgctttata cttttttcct gagatcattc cggaaattgc ccgaatcatg aaaccaagcg
    24241 gtttttttat tacgattacg cattccagca acgacatgcg ggaaatcgtt gacatcgtca
    24301 aaagaattct cgggcgacat catgcttccg gaaccaaccg gtctctgccg atagaaatta
    24361 ttttcgatca gttttcggct gaaaacggcg agtccttgct tcagcctttc ttcaagacga
    24421 tagaggccat cgactttaaa aatacgctgg tatttcagtc tcaggaaatc agatatttca
    24481 tggactattt tcaattcaag aaatcttttt tcctctcgga gcaggacgcc aacaatcaaa
    24541 cgatcatccg tcagctcttc aaggaattgc aggaaacact atcgaagaat caaattgtca
    24601 gtatgtgcaa agacgacaga attttcatct gttcgcaacc ttcaaccggc aaggactccc
    24661 catgaaaaaa cagcgcaagc attgccttta ttgcggcggc aaaaccgtca aaaaatatga
    24721 agataacgtc cggcgcgatt tctgtcccgt ttgtaattct tatttttatg aaaatccgct
    24781 gccggttgtt tcctcaattc tggaagattc ccgtcagatt cttctggtta aaagaggcaa
    24841 gcgcccgtca aaaggattgt ggtgcctgcc gacgggattt gccgaagccg gagaaagtat
    24901 cgaggaagcg gccctgcgcg aattggaaga agaaagcggc ataaaaggaa aaattataaa
    24961 attactcgac gtcgattcct ataaaagtcg cttttacggt gatttgctgt ttctgacatt
    25021 tgtcgttcaa caaacgggcg gaaagctttg cgccggcgat gattgctcac aggcccgatt
    25081 ctggcctgtc cataaattac ctcccttagc cttccgctcc aacaaacgcg ctctggatgc
    25141 ctacattaaa agcagaaaag actactgggc tatttttgat tccatagagc acgcggacaa
    25201 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccggt tttgaaaaag
    25321 attttgtcga atcggtcaaa gagggcatca tgaagcagga gcagtacgac tggttctgca
    25381 aggcaaccaa ttctctttac ggagctacgc cggaacgtca actggcgttt gcctgcaaga
    25441 ataccggttt tttcgctatg gcgttgatgc tcgctgccaa agatgccggc cttgattctc
    25501 acccgatgga cggattcgat attgacggcg ttcacaaagc gtttaatatc cccgaccagt
    25561 actggattcc ctgcttattg gccataggcc atttcgacaa aacaaaaacc cttcttccgc
    25621 caaaatggag aaagagttat gaagaaatcg tgtactgaaa aataaccaat tgccattttt
    25681 ataaaatccg gttaaataag aattactaaa ttcaaggaga agaagatggc cgctaaaatt
    25741 aaaatttaca cgttgagcac atgcagtcac tgcaaggcaa caaagaaatt cctgaacgac
    25801 aacagccttc cttttgattg cgtggatgtc gatttgctgc agggcgatgc ccgtcagaaa
    25861 atgctggaag aagttatgca gtacaatccc gaccgttctt tcccgaccat tctgattggt
    25921 gacaaggtca tcatcggttt caaggaaaac gctattaagg aggctttggg tatttaatga
    25981 ctcctgctga actctacgac atgatgaaaa aggtccagga accgaaaggt tactttttca
    26041 ataaagataa agattgggtt ttaagcatcc tcggcgatct tttagtcaac aaagaacgtt
    26101 atggctatat gtgttgtccc tgccgtctgg cggctggcaa ccgggaaaag gacgccgata
    26161 ttatctgtcc ctgccaatac cggccggacg atgtcaggga atacggcagt tgtttctgta
    26221 atttatacgt ttcagccgaa tggaatgaag gaaaaatcga acatcagtac gttccggaaa
    26281 gaaggcccaa ggaaaaaatc ggcttttaag tccttgtcaa ttcaatgatt ttcaaattct
    26341 gtcggaaggc cgggcaattt ttttcatctt ttctcttgat ttccgtaaat aaattattat
    26401 attcaagcaa aacttaatca ttaaggagtt ttctccatga taaatatgta taagtgcaag
    26461 aaaaagaaca ctctgatcag tgaagtctgc accgatacaa cctgcgaatg gcgtctgaaa
    26521 aacgaatcct ttctaaattg cacatgggtc gcctgcaatt acggtccgtt tacgctggaa
    26581 gaagtcggcg acatgatggg cgttacccgc gagcgaattc gtcagattga agccaaagct
    26641 ctgaagaaac tccaacataa aaagagacgt gatcaactca aagattttgc cgcaccgggt
    26701 aacgactggg acagcctgta actctatctc gccttcacag tcaatattct atacctgcca
    26761 gtttctcttc ggagatattt ttgtgacaca tccgttcggg ataaattaaa ccaaaaaatt
    26821 gtatgctatt caggggaatt gattttcagg tctgatctat gggccttgat tttttgcggt
    26881 gtacttttat gagcattatg attgttcagt tatgactcac ggatttctgt tcgcgccgct
    26941 ccagaatctg ttttctctat tttcttcctg atttatcttt tgaaagcact tctctcattt
    27001 gctcttttcg ctcatcgcgt gtaaagagca gagcgcagat tgacgattca atatccatgg
    27061 ccgccatttt atccatgcgg ggaacgatgg agcacagatt tttcaattcc tcaaaagctc
    27121 tcggagaacg ttgtgacaaa gtatccgcga tactctccgc cacttccatg aatctttccg
    27181 gcggttccac tttattgacc agtcccagtt tcatagcttc attggcgccg atcggttctc
    27241 ccagcataac gatttccttc gccttggtga tgccgacaat ctgactcagc gggtaaaaaa
    27301 acggattaaa tccgaatttc aattccggat gacagaagat ggcgctttcg gatgcgacga
    27361 caagatcgca aacggtaacg agattaaaac ccccgcccag agcgatcccg cccacggcag
    27421 caatcaccgg ttttttaaac gcatatatct ttttcaagta catcatcatg gacttcagga
    27481 atttatcgct ttctttatcg ggcaggttgt tcatttcctt gatatccata cctgccgaaa
    27541 agacatactc tccgccggtc agcacgactg cccgcacatc ggaatcggtt tccagatcgg
    27601 caaatacatc atacaattcc tgatgcatct ctttgctgat cgcattcatt tcatgagggc
    27661 gattcaactt aatcacggcg tacttctttt tcttttcaac aatcaaacta tggtaagtca
    27721 ttttttatta tcaaccatga tacgaagttc ttttcctgtt ttgaaaaacg gaacttttct
    27781 ttctgtcagt ttcacctgag tgcctgtttt gggattgcgg cccatgcgcg gttttctttc
    27841 ccggacctcg aaactgccga accccctcaa ctcaatgcgt tcgccgcgtt ttatcgcgtc
    27901 aatcatcgaa tcaaaaatga tgttgacaac gtacgttaca tccttgaggg aatacgtttt
    27961 gagttcttcc tgtaattttt tgatcaaatc gttttttgtc atgataaact ttcctttatc
    28021 ttccgggata atataagtaa cttaaaccgc tattcatctc cattttttcc ttgagcgacg
    28081 cgcttatttt actcgccgca ccttccagaa cataatcgaa aatcgacgct cttttctttg
    28141 aaggataaac cagatcgaat tcgccgttgc cccctgctaa tttatccgcg agctttgcgg
    28201 cggaagacat gtcgcccaaa taatcaatca atcccaattc cttcgcttta cggcctgaaa
    28261 aaacccgtcc atcggcgatt tcgatcactt tttcccggga cagttttctt tctttcacaa
    28321 tcacgtccac gaactgattg taaatatcgt ccaccagatc ctgaacgatg actttttctt
    28381 caggcgtcat gtcccgtaac ggagagccga tatccttgta ttcgccgctt ttcaccacgg
    28441 cggattttac gccgactttc ttgagcagct cttcgaaatt ggcaaactgc atgatagccg
    28501 aaatactgcc ggttatggtg ccgggattgg cgacaatttt atcggctgcg cacgcgataa
    28561 gcaacccgcc ggacgctgca atcgatccca tggaaacgac aactttcttt gtttttttga
    28621 gctcgaggat ggcatcgtat atttcctgag aagcggcaac gcctcctccc ggcgaatcca
    28681 ccctgagaac caccgcagta atagagcggt ctttgccaaa tttatccaac tgttcgacaa
    28741 tttccagcga atcggcaatc atgccttcaa caggcacaac gccgattttg ttttttaacg
    28801 aaaaccggga tgtctttccc atgttaaaac cggcgttgta aaaagacaga tagaagatca
    28861 gaccaaacac aaaaaggatc aacattccaa acaacacggg atgttttcgc atggtttact
    28921 cgcccttttg tttcgtatta atttttacgt cagcaagagc ttggctgaga ttggaaccga
    28981 cattttccgt attattaata tactgattgg atgacggggt cggcgtcggt gcttccagtt
    29041 ctttgatgct caatctgatt tttttcgatt tcggatcgat actcttcacg acagcggaaa
    29101 ccgtatcgcc taccgaataa agttccgaag atgtcttgac gcgcttctga ctgatttcag
    29161 aaatatgcac caatccttcg atgccttcct caatttccat aaaaatgccg aaatcggtaa
    29221 agttggttat cttgccggtc acaacactgc ccggagcata tttctgggcc agttcatccc
    29281 atggattctt ctcgatctgc ttgacgccga gagagaactt ttcattctgc acgtcaatat
    29341 tcaagacgac tgcctcaact tcctggcctt ttttgaaata ttcggagggg tgattaaccc
    29401 ggtgtttcca ggaaatatcc gacacgtgaa tcagaccgtc aattttttct tcaattccga
    29461 caaacacgcc gaaattcgtg atatttctga caatgccttt tacgattgtg ccaaccggat
    29521 atttttcttt gagcttttcc cagggattcg gcgtcgtctg tttcaggctc agggaaattc
    29581 tcttgttctg agcattgacc tccagcacct tgacgtcgat ttcttctccg atattgagca
    29641 ctttagacgg atgttttatt tctcttgtcc agtacatctc cgagatatga accagacctt
    29701 caacaccgga tgccagttcc acaaaaacac cgtaatcggt caaattgaca accttgcctc
    29761 ttacaataga gttcaccgga taggtttccg ttattttttc ccagggattt gccgtcaact
    29821 gcttcaggcc cagagatacc ctttccttct ccgcatcaaa ggaaagaact ttggtcgtaa
    29881 ttttatcgcc tttctgaaac atttccgacg gcttgacaat tctcccccaa gaaatatcgg
    29941 tcacgtgcag cagtccgtca acaccgccca gatcgatgaa aacaccgtaa tccgtaatat
    30001 tcttgatgat cccttcaaca acgttgcctt cctggatgtt tttcagaata tcctgctttt
    30061 ccgcttctct ttccgaagcc acaatggagc gtcgggacaa aaccacattg ttgcgctttc
    30121 tgtcgaactt cagcacttta aaatccagcg tctgacctac gaatttatcc agatccttta
    30181 ccggacggat atccacctgc gatcctggaa gaaaagcgat aatgccgata tcaacggata
    30241 atcctccctt gaccttttcc acgacaacgc cttccacgac ggtatcattt tcgcaggcat
    30301 ttttaatttc atcccatatt tttacttttg acgccttgtc acgggacaaa accagatttc
    30361 cttccgagtc tcttctgtca ataaacacat cgatttcatc gtcgatggaa atggttacgt
    30421 tgccctgcgc gtctctgact tcgtcgatcg gaataaaacc ttccgttttc cagccgacat
    30481 cgaccatgat ccgatcgtct tttatctgca caacttttcc ggtggctata tcaccgtact
    30541 gaaattgatt aaggctttgt tcgtaaagct ctttaaaatc catttcttct ttaccggaag
    30601 ttatattgtt cttcggtaag gatttttcac ctgcttcatt tttatttgat aagtttgagt
    30661 tgttattgtt aaccattaaa ccaatacccc ctgtttttta tgaaacccag taatttttaa
    30721 ggtttaacat accaaatatt aaatatcaac ataaatcatg caagtaaaat ttatttaaat
    30781 accaggctac ttttttgaaa tcatggacat aattttttcg gcaacctcct caatcgacaa
    30841 attatcggaa tcgacgatga tcgacccttc gggcggcttc aacggcgcaa tggccctctc
    30901 ctgatcctgt ttgtctctga tcgacatatc gttttgaatt gactgatatt ctgccgtcga
    30961 acctttgagt aagagttcct tatgccgtct ttttgttctt tcggcaagtg tcgcatccaa
    31021 ataaaattta taatcggcat cctggaaaac caccgaaccc atatctcttc cttcggcgac
    31081 aagacctccc cgcaatccgg cttcccgctg caggtcaagc agtttttcgc gaacaaccac
    31141 aaaggtggat attttagagg caatcagacc gacttcttcc gtccggattc tgttttcaac
    31201 atcctcaccg tccacgtaaa ccctcatccg gccgttgaca tttttcaaat cgactttcgt
    31261 ggttgaacat aattcggaca gctctgcgct gctgtcaatg gagattcgga tttttaaggc
    31321 cttataggcc agacctctgt aaagagcccc tgtatcgagg taaatataac ccagtcgctt
    31381 tgccaggatc ttgctgatcg tgcttttgcc cgctccggca ggaccatcta tggtgatcac
    31441 agcttttttc ttcatcacgt ataatccttt ttgttatttt taaatgaaaa cttgtcttta
    31501 aaatccaaag aatgtaacat tgcttgaaat gtgaaatcgg aaaatgctta tgtgcaaatt
    31561 cattcaaaat caatatattt atttgggcat gattgtattg atgctgctgt tgacgtcgga
    31621 catttgtcgt gctgaattta ccattgagga tgaaaacaaa ctgggcaggg aattctatga
    31681 gcaactagaa cagcatcaga tgattctcca taataaagtg ctcacagatt atatcaccgg
    31741 tatcggcaat ctgattctgg atcaaaccac ccggacacct ttcaaattta atttttttat
    31801 cctcgacagt tccgccatca atgcgttcgc cactcccgga ggttatattt acatcaataa
    31861 aggattgctg ctggttgcgg agaatgaagc ccagttggcc ggtgtcatcg cgcacgaaat
    31921 cggtcacgcc aatgcgcgtc atgtcgccag cattatttcc aaatctcaga aattaaatat
    31981 ggcggcgctg gctgccatgc tggccggtgt tttcctcggc ggaggaggag aagcatcggc
    32041 cgcgattacc gcattttctc tggccgggtc cagcagcatg acgttgcgct atatgcgtga
    32101 gcatgaagaa gaagccgacc gcctgggaat agattatctc gtccacgcag gatataatcc
    32161 gtcggcgatg gtcgattttc tgaaaatcat gaaacagtac gaatttctct ccaagaccat
    32221 gccatcttat ctacagacac atcccggaac cgatgaccgt atcatttaca tggatggttt
    32281 gattatgact cgataccggc agccagggaa aaacagcatc atcggcaacc tgtgtcgggt
    32341 ccaggcaagc gttcctttaa gcgcctccga actgtcaaga cggcaaagac aactggagac
    32401 gtctttagcc aaagacccgc aaaataccga cctgctctac aacctggcca tgatcgaaga
    32461 tcagttggga gccaacagct ctgctcttga gcatcttcag aaagccctcg ttcaatcgcc
    32521 tgacgatcac gagattctga aaagcatcgg cctgcttcat ctcaaaaccg gccgatatga
    32581 acaggcgcag aactatctcc tgcgtgcaaa aaaatcagac ccccgcaatg atgaaattac
    32641 ctttgcgctc gggaaaacgt atttctcgtc cggaaaatac cgggaagcgc tggattgttt
    32701 tttgaatatc cgggacaggg cgtctaatga ggcggccaat ctaaactact ttacggccat
    32761 gagctacggc aaactccaaa ataaagggga atcgcattat tatttcggtt tgcatttcaa
    32821 gcaggccaac aaaaaagaca gcgccctttt tcatttcaga gaagccctga attttttccc
    32881 cccgggttcg gaacgaaacg ccgaaattca ccgggccgtc aaagaactga cggcaggcaa
    32941 agacaagcaa aaaagaacaa taaaaaaaga tgaacaaaat tcgtttctat gaccggatcg
    33001 aacttgactt cgtcagaagt ctgcatattt taacgaaaaa gaggattcca taaaatgccc
    33061 atatatgaat ttcgatgcaa aaagtgcaaa catatctttg aaagcctgat tttctctcct
    33121 aaagaagaaa aaggcatgat ctgtcccaaa tgcaaagcga aaaatgcgga aaaaatcatg
    33181 tcggtttttg cgggcggaaa atccggctgc tcatcgtgca catcaacgtc atgttccggt
    33241 tgttcttcct gcggcggcca ttgagtcgtc cgtatccggt tttggcgttt tttccggttc
    33301 tttcttttat tattgctaaa atgcttacaa tacattataa gactttcact ttaatattta
    33361 ccatgaaagg agtctcacaa cgtgaacatt ttaatttttg gccccaacgg cagcggcaaa
    33421 ggaactcagg gcgcaatcgt gcagaagaag tacggcgtgc cccatattga aaccggcgtc
    33481 attttccggc agaatatttc gaagaaaacg ccgctgggag aaaaagcaaa ggcttttatt
    33541 gaccgcggcg aactcgtccc tgatgatatt accatcccca tgattctgga ccgtctcaag
    33601 gaagacgatt gcaaaaaagg atggcttctg gacggcttcc cccgcaatct cgctcaggct
    33661 gaagcgctct gggatgctct gcagaaagaa aacatcaaac tggattccgt catcgaaatc
    33721 gttctggacc gccagattgc caagaaccgc atcatgggac gcagactttg caaaatcgac
    33781 aacaaccatc ccaacaacat tttcattgat gccatcaaac cgcatgagca tgacggcaaa
    33841 accgtatgcc gggtctgcgg ttgcgaagat ctgtccaccc gcgccgacga tcaggataca
    33901 gccgccatcg acaaacgtca cggcatctac tacgacacca agaccggcac actggccgct
    33961 gtgaatttct acaaggcaaa agtgaaagtt attgaagttg acggtagcgt tggcgtgaaa
    34021 gaagttacag atgaactgat gaagaaactc ggataaaaac ttaagacacg tgacaaaaaa
    34081 agccccccag cattccttgg agggcttttt ttaaaaatac tttatggaaa acataagaaa
    34141 tttcagcatt atcgcccata ttgatcacgg caaatcaacg cttgccgacc gtctgattca
    34201 atttaccggc gtctgcaatg aaagaaattt ccaggatcag attctggaca atatggatat
    34261 cgaacgggaa cggggcatta ccattaaaag caatgccatc tccctgccct atcgcgccaa
    34321 agatggaaaa acgtacatcc tcaatctgat cgatacaccg ggacatgttg atttttcata
    34381 tgaggtttcc cgatcgctgg catcctgtga aggcgttttg cttctgatcg atgccgccca
    34441 gggcgtgcag gcgcaaacac tggcgaatct ttacatggcg ctggaacatg atctggaaat
    34501 cattcccgtc atcaataaaa tcgatctgcc ctctgccgac attgaccgcg tactggaaga
    34561 aattgattcg gaattggggc ttgatccctt cacgcatatc aaatgctccg ccaaggaagg
    34621 cgccgggatt gaagaagttc tcgaagcgat tgtcctgcgc atcccgccgc ccaagggaag
    34681 ccccgcaaat cctctggccg ctctgatttt cgacgccaaa tacgattcct tccgcggcac
    34741 aattatccac tgccgcgtct ttgaaggaac catcaaaagc ggcgacatca tcaaatttat
    34801 gtccaacggc gccacttaca aagtggagga agtgggacat tttctgctga accgcaccaa
    34861 acgcgaaaaa ctttccgccg gtgacgtcgg ctacatcatc gccggcgtca aaaccgtttc
    34921 tgatgtgcgc accggtgaca ccatcacgct tcagaacagg ccatgcgttc agcctttgcc
    34981 cggtttcaag gacgtcaaac cggttgtttt tgcttccatc tatcccatcg cgtccgatga
    35041 ttatcaggat ctggccgact cgctcgaaaa atacaagctc aatgatgcat cttttattta
    35101 tgaaaaagat tcttcggccg ccctgggaca gggcttccgt tgtggatttt taggcctgct
    35161 tcatcttgat atcgtacagg aaagactgga acgggaattt aacctctcca ttattctttc
    35221 cgtacccagc gtacgctacc gcttttcgct cacagacaaa accgtcattt acgtggacaa
    35281 tcccgcgcat tatcccgatc cgatcgaaat cgacaaaggt gaggagcctt acattcgctc
    35341 ctccatgatg cttccggaac gctacctggg agcggtcatg aaactgtgta tggaaaaacg
    35401 cggcgtgaac tctgtcatga attatactgg tcccggtcgt gtggaattat cctacgagat
    35461 gccgcttgcc gaagtgattt atgattttta tgaccgcttc aaatccgtca cccagggcta
    35521 tggctctttt gattacgaca tcatcgatta ccgcgagagc aatctggttt taatggacat
    35581 cctggtcaac ggtgaaaaag tcgatgcgct ctcccagatc gctcatcgag atcgggcgcg
    35641 cgcaagagct ctgctggcct gtgaaagact gaaggaggag attcctcgcc agatgttcaa
    35701 gatcgccatt cagggtgcca tcggcggtga aatcattgcg cgcaccacca tcagcgcctt
    35761 tcgcaaggat gtgacagcca aatgctatgg cggcgacatt acgcgcaaac gaaaactgct
    35821 cgaaaaacaa aaagcgggaa aaaaacgtat gaagatgatc ggttcggtca gcataccgca
    35881 gagtgcattt ctggcagttc tgaaatccga taccgactag atcttgacac caaccgatct
    35941 tttctttgat tgattcttat gcgaagcgaa aaagccggcc tttacattca cgttcctttt
    36001 tgcctgagca agtgcgccta ttgcgatttc tattccgtca aaacgcttca cctgattcct
    36061 gactatatcg cggccgtcca aaaggaaatg aaactgtatc gcaacgtctt ttcttcattc
    36121 gattccatct atatcggcgg cggcacgcca tccctgctgg ccccgcaata cctggatgaa
    36181 atattcacag cattaaacaa atattttatc attgataaaa acgctgaaat cacactggag
    36241 gccaatccgg gagatctttc cccggtatat ttgaaagcgc tgcgcgacac agggattaat
    36301 cgtctgaata tcggggttca atcttttaac gataagattt taaaatgcct tggccgacgg
    36361 cattcggctc aggaagcgat ggcgtcgata aacgccgcca gaaaagccgg attcagaaat
    36421 ctgggtatcg acctgattta cggcgtttac ggactgggtc ttcaaggatg ggaaaaaatt
    36481 ctgcaacagg cgttgatact ggcgcctgag catttctcct gctaccagct ttccctccac
    36541 gaaaaaacac cgcttcacaa aacatatctt tccgccggat ggcatcttcc ggatgacaat
    36601 gcggaactga aatttttttt aacgacatcc gaaattttgg aaaatgtcgg ttaccggcat
    36661 tacgaagtct ccaactttgc ccgaaaagac aaatatacat cccgtcataa tcagaaatac
    36721 tggcagcacg ttccctatct tggtctggga ccggccgcga actcttttct gaataatcaa
    36781 cgatggtgga acaaggcatc tgttaaacgt tatttaaacg ccatggcccg cggcaagacg
    36841 cccgttgaag catcagaaac acttaccgtc aatcaactgc ggctggaagc tctgtttctg
    36901 gggctacgct gcaaatccgg catcgatcta aagcgcttca aagatcaata cggcaccgat
    36961 ttgatgactg aaaaaaaatc tattatggat gctttaatga aaaacaagct catagagtta
    37021 aaaaacggtt ttctttgccc gacgcgttcc ggaatggctc ttgccgacag cctggcttta
    37081 atctgagtgg cagctgatac agaacttgac atatcggcta caagtacctg ttttacatta
    37141 cgtatataaa tagacagaca ggaagtgtct gtaagataat gtatcaccgg atggttacag
    37201 aaggaatctg ctgctcaagt tgttttttaa tttttcgcag tttctcttcc gaaaaagatt
    37261 tttccttcgc gaacttttta tccagtgtct tcttcgccat aaaattctgc agcacgtaat
    37321 gaccggcgcc gttgatcatt tttccgatct gcaagatatc gttttcatcg agctgagatt
    37381 ccacaatcgt tgtgcggaat tcatagggta tctttgcgtt taaaaccagc cggatgcttt
    37441 cctgaatagc atcctgattc acattggcct tgacaatgtc cctgtacttg tctagaggcg
    37501 ccttgacatc catggcgatg aaatccagca tgttgtcggc aatgagggtt ttgagaacat
    37561 tcggcacaga gccgttggta tccagtttaa ccgcaaatcc tatttttttg atctgcctga
    37621 tgaaagagat cacattatcc tgaatggtcg gttcgccccc gcaaatcgat actgcgtcaa
    37681 gttttccttt tcgcgattca agaaaatcaa tcacctcact gactttgata ggtgtgcgaa
    37741 ataaatcggg attgaccaat tcgggattat ggcaataggg acatttgaaa ttacatccct
    37801 gggtgaatat aattgcggaa atataccccg gataatcaat aagggacact ctctgcaaac
    37861 cgccgatctt catcatacgc ctctcataga ttaaaaaaac aaacgcgccg ccgcgtctga
    37921 tgattctaga ctctcatgat ccgtttattt gaatttgaat tcctggcgca agtcaaactc
    37981 ttcctgcttg cctttattcc actgcttgac cggccttaag taaccgacga cgcgggaata
    38041 gacttcacaa tcatccgcgc aggtgggaca cttctcctgt tcgcctttaa tgtatccatg
    38101 agacggacag acgctgaatg tgggagtgaa ggtgatgtac ggcagatgat aattgaaaca
    38161 aatcttctga accagtttct tgacggattg aggatcgtca attctttcgc cggcaaatat
    38221 gtgaaacacc gtgccgccgg tatatttgac ctgaatttca tcctgcaaat ccagcgcttc
    38281 aaagatatcg tcggtataat ccaccggcaa ctgggtggaa ttggtgtaaa acggttccgc
    38341 ctttttcgat ttgccttcgc tggccgtaat aatatccgtg aacttctctt tatcaatttt
    38401 cgccaaccgg tacgacgtac cctctgccgg cgtcgattcc agattataat tattaccggt
    38461 ttctttctgg aaactgatca atttgtttcg catgaaatcc aaaacctgct tggtgaatac
    38521 ctgtccctta tccgtcgcaa cgttcacgcc cagcagattc agacaagcct cgttcatgcc
    38581 gaccagtccg atcgtggaga aatgattctt ccagtactcg ccgaaccgtt ctttcactcc
    38641 gcgcagataa tacttcgtat agggataaag atttccgtac gtgaacttct ccagcacctt
    38701 tctcttggtc tccagacttt ccttcgccag ttccatcaga tgctccagac gcgtcaaaaa
    38761 atcctttttg gattttgaaa gataagcaat ccgcggcatg tttatcgtaa tgacaccgat
    38821 agatccggtc agaggattgg agccgaacaa accgccgcct ctcatttcca gctcgcggtt
    38881 gtcgattctc aggcggcagc acatgctgcg ggcgtcttca ggattcatgt ccgaattaat
    38941 aaaattggaa aaatagggaa cgccgtactt ggctgtcatt tcccagagat cctgcaagtt
    39001 gggatcgtcc cagttaaagt ccttggtaac gttgtaggtg ggaattggaa atgtaaacac
    39061 cctccccttg gcgtcgcctt ccgccatcac ctgcaaaaaa gccttattga aaagattcat
    39121 ttcttcctga aattctctgt aggtctcttt ctgcggcttt ccgcctatga ttacattctg
    39181 gtcggcgtag tatttcggca ctgttacatc cagggtgaca tttgtaaaag gcgtttgaaa
    39241 tccaaccctt gtcggcacat taatattgaa aataaattcc tgaagggcct gttttatttc
    39301 tttcatagac aaattatcgt agcggatgaa tggagcaagc aaggtatcaa aattggaaaa
    39361 ggcctgagcg ccggcggctt cgccctgcaa cgtataaaag aaattcacca cctgtcccag
    39421 cgcgctgcgc aagtgattcg ccggtttgct ctccactttt ccggacacgc cttgaaatcc
    39481 ctgcagcagc agatcataaa gatcccaccc cacgcaataa acggacagca gactcaaatc
    39541 gtgaatgtga aaatcgcctt ccgtatgcgc tttgtgaatt tccggcgtgt aaatttcatt
    39601 gagccagtaa accttgctga cttccgaaga aatataatta ttcaatccct gcagggagta
    39661 atccatgttg ctgttttcat tgatcttcca gtcaagcttc ttcaggtatt gatcgacgag
    39721 atcaacttcc attcgattgg cgatatctct caaacgggag tgctgatctc gataaatgat
    39781 gtacgccttt gctgtcttgc ggtacggaga gctcagcaga acttcttcaa caacatcctg
    39841 aatttcttct acggacagga ttttgctgtc gaagagtttc tcggccagat tcaggacttt
    39901 gatcgttaat ttacgcgcct ctttgatgtc aaaatcgcct gtggcatttc cggccttggc
    39961 aatggcattg gtaatttttt ccgcgttaaa tttaaccagt cgcccgtctc tttttctaat
    40021 tttaagattc ataaaaacac tccatccgat gaaaaaaata atataagaat aaatccaaaa
    40081 tagcgctata tattggggtt gaaaaggata gtaccactat atatagtaat tttcaaggga
    40141 ataataaata atttttaaag tggctattcc gatggaaact caaagagata ggctgatttt
    40201 agtgcaaaaa aaatacgaat tacgcttaaa tcttgttgta ttcggaaaag atcatggaga
    40261 aaacggccct cccctgtgtc gaggacctca aatcggtgga atacccgaac atggccttca
    40321 agggaacctt cgccttaatt tcgctgaccg ttcctttggg attaacggcc tgaatttcac
    40381 ccttccgggc attaatgtcg ccgatcacat ctcccatgaa ctcgctgggc gttatcacat
    40441 cgaccatcat aatcggttca agcatgatgg gctccgcctg accgcagccg tcgcgaaatg
    40501 cggttgaagc ggctatttta tacccctgcg ctgaagattc gccctcacgg aaagaaccgc
    40561 cgataatcgc gacttccgta tccatgacag ggtaaccatt taacacgccg ttcatcattg
    40621 cttcccggat gccctcctcg atgacttcat ggaaatcctg aggaatgacg ctatcgtcaa
    40681 gttttatgat cacgtcattt cccgaacctc tcttgcgcgg ggacagggca atccggacgt
    40741 gaccgaaatg cttcttttcg ccaagttctt tttcaaatat accttcgatt tccactttct
    40801 tctggatggt ttcgcggtag acaaccctgg gccggcccac attcacatgg cagttgaatt
    40861 cccgcagcaa acggtcaaca ataatttcca gatgcagttc ccccatgccg gaaatgatcg
    40921 tctgggcggt ttcatcttcg tatttcacct tcaacgtcgg atcctcttcc accagcttat
    40981 ccagcgccgc cgtcagtttt tcctgatcgg ctggtgtttt ggcttcaatg gcctgactga
    41041 tgaccggttc ataaaattca atcagttcca gtaaaatcgg cttctgctcc gcgcaaagcg
    41101 tatcgccggt ggtagtggat ttcaatccca gaacggcaat gatatctcct gcggaggccg
    41161 agtctatcct ttcacgtttg ttggcgtgca tgcgtaaaag gcgggctatc ttttcccgcc
    41221 gtttttttat cggattatag atttcgttat tgacctgaat ttttccggaa taaattctca
    41281 gataggtgag ctttcttccc tcgtccatca taattttgaa cgcgagcgct gccaaagggt
    41341 ccttctcgga acttcgtctg atctcttctt ctttcgttac cggattcatt cctttgacgg
    41401 gcggaatatc ttcaggagac ggcagatagt tgataatggc atccagaacg ggttgtatcc
    41461 ctttgttgcg cagggctgag ccgcacaaga ccggaacaat tttcagagat atcgtcgctt
    41521 ttctcagcgc ctcttctatc tcagcaatgg taatttcttc accgcccagg tacttatcgg
    41581 caaggctgtc gtcaacctcg gcaagcgttt cgatcatttt atcgcgctgc aacttcacgt
    41641 gatcggcaaa ttccgacggc acgtcctgct tagacatttc cgacccggat gtcgaatcgt
    41701 tccagacgat cattttctga ttaatcagat ccagcacgcc acgaaagtta tcttcactgc
    41761 cggcgggaat ttgaatggcg acgggcagag aggaaaaacg ttcccgcatc attccaatca
    41821 cattaaaata atcggctccc actcggtcca ttttgttaat aaacgcgatt ttcggaacac
    41881 cgtatttgtc agcctggtgc cagacggttt cggattgcgg ttcgacaccg cccaccgcgc
    41941 agaagacgac cacggccccg tccagcacgc gcaatgaacg ttccacttct atggtaaaat
    42001 ccacatgccc cggtgtatcg atgacatgaa tgtcatgatt gcgccagaca caggtcgtca
    42061 cggccgatgt tatggtgatg cctctttcct gctcctgcgg catccagtcc attaccgctt
    42121 caccgtcatg cacctcgccc atcttgtagg atttgccggt atagtaaagg attctttccg
    42181 taaccgttgt ctttccggcg tcaatgtgtg cgacaatacc gatattgcgc gtccgggcta
    42241 tttttgattt ggatgccata tgcgtcgatt aattgttagg ggattgctcc gggataatga
    42301 gttcgagatc cggcgaaaaa aacttgcctg ttgaaatgtg gccctgaatc gaggaaagct
    42361 ccttaccgtc agccagaatt tttactcttt tgatttccgg aatattctgc gtgatggaat
    42421 tcgttaacga ataaatcgta ttcatctccg cggcagaact gccttgatac gccttgatca
    42481 gatctttgct gaaattaaca tacgcaacat catgatcctt aattttgaca tcccggagcg
    42541 aaacgttttt gggaaaggta ttcaccagtc ccgtcttgga accgtcaagc agagccttga
    42601 cgatttcctg agcctgcaac gcaatgtcgc tttccttgat gacatggcgc ttttcagcct
    42661 tcaaaaaacg ctcctgctga tccgaaaaat aaattttaac aacccgcctt tcctttttgg
    42721 tcatgacgtc cgtatcgacg ggggggaaca gtgcgctgaa tatggtaata aaaaaaatga
    42781 cgagcaagac tacaccgccg ccaacaatca gcgaaaggta aaatacccgg gttgattttt
    42841 ttatcttctt ttccttgatg ctctctgacc ttagctgttt cttggtcgac ataaaatact
    42901 ccattcctca gaaaaacgct gccaatctat ggcattttat atgaactgtc aagaagaacg
    42961 agcgttgata aaaaaaacag cccctgcatt gagacatcag aaacgcagga agctgtttta
    43021 ttcttacaac tctatcgttc tgttcgcctg aaaatcctta atggctcatg atccccttcc
    43081 tacttggctg ccgggatagt ttccaattcc gatttatcag aattggatag ttcgacttac
    43141 gcctcgagct tcgtttcact cgccctcagc ttgtctcaag acccccattc gcttcgctca
    43201 ca
//
LOCUS       DAONQZ010000016        40787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1073_length_40787_cov_14.933615, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000016 DAONQZ010000000
VERSION     DAONQZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 40787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40787
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_40787_cov_14.933615"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..326
                     /locus_tag="PLR13_04540"
     CDS             96..326
                     /locus_tag="PLR13_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQC18634.1"
                     /translation="MEKIIKIHVKKLPEGVYLATSKDVPGLVAQGRTATETLEIARDV
                     AKKLLEARKERRGKIKISEIKDSFDYPMIVSA"
     gene            923..2416
                     /locus_tag="PLR13_04545"
     CDS             923..2416
                     /locus_tag="PLR13_04545"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQC18635.1"
                     /translation="MNVFVKKGMLAETKSQAVILTLFEDGKKLSGIALEIDKKSGGLI
                     SKLMADGDFEGKASQVAVAYPCGNLPSKRIALVGLGKQKEFNLEKLRAAFAKVMRHLR
                     GMNVKEASTSVNLNLIPGKKDQIVQAAVEGALLGLYQYTPYKTVGREEFKEMDEFNII
                     ADAKEYAVIEPSVKKAKIIADAVCFVRDMISAPSNEMTPSIMAQKAQEVAKRKNVTCT
                     VLDRKKMQELGMNALLAVASGSQEEPKFIILEYSGGRKKDAPVVLVGKGLTFDSGGIS
                     LKPSDKMEEMKTDMSGGAAVMGAIMAAADMQIPLNVVALIPASDNMPSGSAFKPGDIL
                     KSYSGKTIEILSTDAEGRLLLADALAYASKFKPEAVIDLATLTGACVIALGDDVIGML
                     GTGEKLKKEINRAAQDTGELVWELPLWEQYHELIKSDIADYKNAGNRAAGTITAAAFL
                     SKFVGDYPWAHLDIAGPAWADKDKPYIPKGASGIGVRLLVEFLRNRS"
     gene            2426..3415
                     /locus_tag="PLR13_04550"
     CDS             2426..3415
                     /locus_tag="PLR13_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18636.1"
                     /translation="MPPLLQIKDLRTVFDTVHGRIKAVDGVSLDIASGETLGVVGESG
                     CGKTMLSLSIMRLIPPNGKIINGEILFSGVDLLKLSEEDMRARRGKDIAMIFQEPMTS
                     LNPVFRVGDQIAEAIRLHQQLPAKQAMALSVELLREVGIAEPQKRARDYPHNLSGGMR
                     QRVMIAIAMSCHPQLLLADEPTTALDVTIQAQILDLISNLKQKNNMAVLFITHDLGVV
                     AQAAEKVAVMYAGKIVEYSTVENLFAKPLHPYTQGLLESIPARCSDAKEKQAYLKTIP
                     GSVPDLYRMARSCRFHDRCPFAFAECELQDPPLLAVEDGHLVSCWKYGSEKNN"
     gene            3431..4399
                     /locus_tag="PLR13_04555"
     CDS             3431..4399
                     /locus_tag="PLR13_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC18637.1"
                     /translation="MSSVLVDIQKVDKKYTLGGNVFGAQKRIIHAVKNVDLQIIRGET
                     LGLVGESGCGKSTLARLITRLENPTSGVIAYKGENIFTFTGESLKTYRRSVQIIFQDT
                     YSSLNPRKSALSMIREPLTIHRLGSKKEREETALSIMAKVGLKKEQAHRYPHEFSGGQ
                     RQRIGIARALVLNPELIIADEPVSSLDVSIQAQILNLLRDLKNDFHLTYLFVSHDLNV
                     IRYISDRVAVMYLGKIVELAGGEDIYNRPLHPYTRMLLSAVPSCDPGKRKTSIAIKGE
                     ARETDNEGCAFRNRCPYRIDVCDKTDPALDAINGQSFCACHRAGAI"
     gene            4475..5089
                     /locus_tag="PLR13_04560"
     CDS             4475..5089
                     /locus_tag="PLR13_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18638.1"
                     /translation="MREEKILIKNNALVIEALLHRASGERGAVICHPHSLMGGSMYNN
                     VVEALQEAFASEGISTLRFNFRGVGSSTGSYSEGVGEKEDIWAAGNYLKTSGLREIFF
                     AGYSFGAWVGAGVIAQPDHPFCSSVFVAPPIDYFEFDWPPLKNKIDLMICGDADSFCP
                     WEPLKDASKKINALLEVIGGADHFFMGKEKKLVILLRDYLAKKT"
     gene            5341..5541
                     /locus_tag="PLR13_04565"
     CDS             5341..5541
                     /locus_tag="PLR13_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQC18639.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAITGTGFKTLYEG
                     QKIKFDIEQGNKGPSATNVTPL"
     gene            complement(5683..7026)
                     /locus_tag="PLR13_04570"
     CDS             complement(5683..7026)
                     /locus_tag="PLR13_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQC18640.1"
                     /translation="MNKRLDLGKEAILPLILKMSWPSIIAMTAMSLYNFIDTFWLARF
                     SSQALAALTVCFPIQLLAAAIGVGTGIGAGSFSARMFGAEKNLEAQKTAGQTFFLSML
                     FGFGSILFVLLFGDSMLVFFGASDEILPLSHDYLFLAVYSSPFLFFSMMTAIMLRVEG
                     RPLLSMNVVLIALISSAILDPFLIFGIGPFPRMGIRGAALAGIIAHLIMFILSLYFLQ
                     LKSSKYDFRLKYTRPDLQIIKSIYSTGFPSMVTNFVISIVLVIYNHVLGNFGFQAIAA
                     FGICFRVTGLATMVLFGVGAGLMPIVGYNLGARLFQRLRESVSVALKVSVVFSAVSVI
                     ILELFSSQIITFFSSDPHLTAIAIPALRIHVAALIFSAPIIIYVNMFIGLGKGTLAMI
                     ILFIRDILVFVPLLIILPLFWGITGAWMALPLAGFIELFLVIYWGEKELRKLETR"
     gene            7094..8005
                     /gene="miaA"
                     /locus_tag="PLR13_04575"
     CDS             7094..8005
                     /gene="miaA"
                     /locus_tag="PLR13_04575"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQC18641.1"
                     /translation="MAKNLIVILGPTASGKTRLAAQLACDFQGEVISADSRQVYRTMN
                     IGTGKDLKEYVIDGRQIPYHLIDIEDPENEFNVFEFQKRFYAVFSAIRQRKKLPVLTG
                     GTGLYLEAVLCAYDMPEAPINEEIRRELSEKSIEELQDLLCRLKPHLHNRTDLDDKKR
                     LLRAVEIEKARIRNNQDLRDRPEITAAVFGVRWERSVLRNRITKRLEERLENGMIEEV
                     ADLHAAGLSWLRLESFGLEYRFISEYLQQRLTFDEMKNRLNIAIHQFAKRQETWFRRM
                     ERKGVQINWIDNGDYLLLAEKVAKHLK"
     gene            8002..9357
                     /locus_tag="PLR13_04580"
     CDS             8002..9357
                     /locus_tag="PLR13_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18642.1"
                     /translation="MKKRSAFDDYLHTYSVGEKWKLCAVPDQNFSQVVVIPAYAEKDL
                     LFRTLASLAVNVPDALENSFILCVVNHKEDSPASVMENNKITMAYLNALIRRKSLKTF
                     QLTKDVTALLQCISDSKMKLGYIDAASRGYEIPRNTGGVGMARKIGMDMALRLLEKNA
                     PQNGAIVSLDADTLVEPNYLSVVRDYFRKNVKTAIMAYAHQMPENPAEQAAICCYEIF
                     LRYWVLGLRYAKSPWAFHSIGSTMAVAPQSYLAVRGMNKRAAGEDFYFLNKLAKVGRI
                     DYIRETCVHPSARVSTRVPFGTGKRIERFLAGDAAKEYYLYDPQIFSILSGWLSLVHR
                     MIDRDVDDILMEAKRIHPALKAFLEEARFPLRWSAIRRNTKDQETLLNHFHVWFDGFR
                     TLKMINYFSRVAYPPVDMFSALKRIFNLLDLPALSPEVAEDIPSLGEQMEILRFLRTI
                     T"
     gene            9514..9936
                     /locus_tag="PLR13_04585"
                     /pseudo
     CDS             9514..9936
                     /locus_tag="PLR13_04585"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
     gene            10006..10515
                     /locus_tag="PLR13_04590"
     CDS             10006..10515
                     /locus_tag="PLR13_04590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQC18643.1"
                     /translation="MKPTEKDINKFYNDNREKLFVEPESVHVRHILVSVGKDDSEKVK
                     AEKKAKIDDLRKQLVGGANFAEIAGQHSDCPSKQAGGDLNFIRRGQMVKAFEDAAFAQ
                     EKNAIGPVIKTEYGYHIVQVLDRRPAKKIALSEVKSKIADYLERQKKMEAFNNLMAKL
                     KAQAKITVY"
     gene            complement(10540..11358)
                     /gene="panB"
                     /locus_tag="PLR13_04595"
     CDS             complement(10540..11358)
                     /gene="panB"
                     /locus_tag="PLR13_04595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQC18644.1"
                     /translation="MSTHSKITRKTTADIRKMKTTGEKIAMLTAYDYAMASILDESDI
                     DMLLVGDSLGNVMLGYDSTLPVTMDDMLHHTKAVARAVKKAMVVADMPFLSYQVSPEA
                     ALANAGRFLKEADAQAVKLEGGREFADIVHKMTYAGIPVMAHLGLTPQSVHQLGGYKV
                     QGKKDDAAETIMQDARILEESGAFAVVLECVPEKLAAEITSALSIPTIGIGAGVYCDG
                     QVLVVHDMLGMQEKFTPKFVKKYANLNLEIKKAVKTYIGEVKNSNFPDTEHSFK"
     gene            complement(11345..12241)
                     /locus_tag="PLR13_04600"
     CDS             complement(11345..12241)
                     /locus_tag="PLR13_04600"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HQC18645.1"
                     /translation="MKKKELNNFAIIGTGMVGTAIGSLLKKSGYNVVSICDKSSTALK
                     RAVSYTGGKPCKTIPDAVGHADSILITTPDDHIASACREIASASSIAGKYVFHMSGAG
                     GLDLLESSKKAGAFVASIHPLQSFSSIDQAIKNIPGSYFGVTADRYSLIPAKHIIHKL
                     GGIPMVISNDQKPLYHAAACFASNYLVSLMNIVESIYRSIGIREKDAQRAYLPLVYGS
                     LKNIEKSGSISSLTGPIARGDYGTIRKHLRTIDKNLPRYSSLYASLGLITVKIAQQKG
                     TLNAGQARKINALLKGGKHEHS"
     assembly_gap    12515..12614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12862..13509
                     /locus_tag="PLR13_04605"
     CDS             12862..13509
                     /locus_tag="PLR13_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQC18646.1"
                     /translation="MMKKIDLMIFDLDGTIASTGDDLVLCINHTLKTLNLRERTKKDI
                     IGFVGDGISKLMERALGSRYREYQEEAMRIFSAYYAEHLLDNTKLYPHVEEVLRNFEN
                     KPKVVLTNKRYPYALMIVRGLKIEKYFTEVIGADSTPFLKPDRRIIDSVLEKHPAERH
                     RALIVGDGVNDIAVARNSGIMSCAFLNGLGRRDDLLKAKADYYCEDIREVNRLFR"
     gene            13512..14927
                     /gene="tilS"
                     /locus_tag="PLR13_04610"
     CDS             13512..14927
                     /gene="tilS"
                     /locus_tag="PLR13_04610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQC18647.1"
                     /translation="MIKKVKQTIEKFDLLKKGERVVVAVSGGPDSTACLTVLASIAGD
                     MDLELIVAHFNHGLRGKESDRDEQFARRLAKKLGLVFYAGKLNKKIRVKGISPEDFYR
                     RQRYAFLEKTAAKHRAQKIALGQHLNDQAETVLLNLLRGSGLEGLKGFLPIREGRIIR
                     PLIEVSAQEIISFLEQSGMAYCRDSSNESSQYLRNQIRAELIPFLKGKYNPRIEENLA
                     QMAEILRREDDFIRQHAAAAAKKTMQQKKAGSVALNIHAVKKIPEAIRWRLLKMLLED
                     LTPSGDGISFLHVNAVNDLTQKNASGKKITLPAGIEARREYDQLILEKKRTRPKKNQY
                     AYVMSVPGTVHVKERGLTIKATLVKKIKTDLSRMKNTACLDWSKIAPPLVVRNRRDGD
                     WIQPLGMRGRQKIKKYFIDHKIPVAQRDDLLLLVDRLSVVYIEHRHISDRVKISPATK
                     SVLKLAITPDRPVGKGSKPPG"
     gene            15005..16828
                     /gene="ftsH"
                     /locus_tag="PLR13_04615"
     CDS             15005..16828
                     /gene="ftsH"
                     /locus_tag="PLR13_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQC18648.1"
                     /translation="MNQFQKNIALVLTVLLVFLLVWQLYNQPKTGTKEIIYSEMLTLL
                     DKGEISEVTIQGDHINGKLKNNSLFKSFAPKDDQLVEKFSGKGVKITAKPVEESAWLT
                     ILISWAPMILLIGIWIFFMRQMQAGGGKAMAFGKSKARLITDKSKRVTFADVAGIDEA
                     KSELEEVIDFLKDPKKYTKLGGRIPKGLLLVGPPGTGKTLLARAIAGEAEVPFLTISG
                     SDFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFESNEGVILISATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREKIFEVH
                     ARKTPIAGDVDFAVLARGTPGTSGADIENLINEAVLNAARANKEKVNMSDFEFAKDKI
                     LMGSERKTMVISDEEKRNTSYHEIGHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLP
                     VDEKHTYPRRYLENSISILLGGRAAEELVLKDFTTGAGNDIERATNLARKMVCEWGMS
                     DKMGPLSYGKKEEQIFLGREFATHKDYSEETAKNIDAEVMAIVARNYERSKQLLTDHI
                     DLLHKISGELLEKEVLSGAEIDTLIGDALPKAHRSEPQTSV"
     gene            16891..18096
                     /gene="folP"
                     /locus_tag="PLR13_04620"
     CDS             16891..18096
                     /gene="folP"
                     /locus_tag="PLR13_04620"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQC18649.1"
                     /translation="MKIIDIQSTQEAAALFRRIGVDAYGIGAMAPKAVNLNILLEKQP
                     CKIANILKQEMLALGGDAAVARGSVACSVETTDVLMMGTLKQLSRLAAKIARQPFGLD
                     SIARDILRILKNAGQKRFILKTSRRKITLGDRTRIMGILNVTPDSFSDGGTFLSAERA
                     VEYGLQMEAEGADILDVGGESTRPGSAAVPVHVELKRVLPVIKALSRKIKIPLSIDTK
                     KARVAREAVEAGAEIINDISALNGDRKMAGTAAETGAALVLMHMRGNPENMQKGNLAY
                     DDLLGDITDYLSASIGKAIRAGVSKDAMVIDPGIGFGKTVADNYRIIRRLADLRTLGM
                     PILVGPSRKSFIGNVTGDEPRQRLEGTAATVSAAIMNGCHIVRVHDVAAMKKVAAVTD
                     AMTHPRKVI"
     gene            18096..18479
                     /gene="acpS"
                     /locus_tag="PLR13_04625"
     CDS             18096..18479
                     /gene="acpS"
                     /locus_tag="PLR13_04625"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQC18650.1"
                     /translation="MVYGIGVDLVENRRMERIIDRWGVKFLNRIFSTEEMAYCGKHAY
                     PVTHYSARFAAKESFLKALGIGLGMGIKLSDISVVNHEGGQPFLNLSGDAQSYIQKRR
                     IHKIHLSLTHTKKYSAAMVLLETID"
     gene            18499..18987
                     /gene="tsaE"
                     /locus_tag="PLR13_04630"
     CDS             18499..18987
                     /gene="tsaE"
                     /locus_tag="PLR13_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQC18651.1"
                     /translation="MSQDDKTVEIISVSTENTMEIGRSIGEGLQDGDVVALSGELGSG
                     KTCFTGGLARGLGVDEKYRITSPTFTLINEYPARHRLYHFDVYRISSYSELDDLGCEE
                     YLGTEGVVVIEWAEKIKKILPEKTVYIDFEYVDENRRKMIIRASADRLNELIKDIKTE
                     GV"
     gene            18990..20210
                     /locus_tag="PLR13_04635"
     CDS             18990..20210
                     /locus_tag="PLR13_04635"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQC18652.1"
                     /translation="MALIVQKFGGTSVANIEKIKNVAEKVIKVKQAGHQVVVVLSAMA
                     GETDRLINLAHSATDLPDEREYDSLISTGEQVTVTLLAIVLNAMGCKARSFLGFQVKI
                     LTDQAYKKARISLIDTENIKKELKKGTAIVIAGFQGVDKDNNITTLGRGGSDTSAVAL
                     AAALRADQCDIYTDVDGVYTTDPNVCNKARRLEKISYDEMLEMAMMGAKVLQPRSVEM
                     AKKYNVPVYVKSTFSDKGGTLVTREDKDMEREIISGVTYDRDQVKITVVHVPDQPGVA
                     ASLFSPLSDKNIFVDMIIQNASSEGYTDLTFTVSKKDLKEAQKIVESTAKKVGAKKIE
                     VDDQVAKISIIGVGMASHSGVAAKMFKTLAAEGVNIMMISTSEIKISCIIQRKYMELA
                     VSVLHDAFGLDKKK"
     gene            20182..20778
                     /locus_tag="PLR13_04640"
     CDS             20182..20778
                     /locus_tag="PLR13_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQC18653.1"
                     /translation="MLSDWIRKNNIVFISESRLKGLIVLSLMVAVIPFVSLMITSWTP
                     YRIPVYADTCDHCCVIEIVGDDQRAGIYHVPSGMSVNRLLKTAGVEKQFEKDFTVKTG
                     MKLVIRSASDRPGITVAEIQNTAKISVGLPIDVNKAGEEDLILIKGIGPVTAKRILEL
                     RTRLNGISDIRQLMEIKGIKEKRMKHIEKYLYVEKKNV"
     gene            complement(20780..21409)
                     /locus_tag="PLR13_04645"
     CDS             complement(20780..21409)
                     /locus_tag="PLR13_04645"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQC18654.1"
                     /translation="MQNNIYRLFKLHITLFSKIVIAALLFFFAAAFTDIARADIYKYE
                     DNEGVLHLTNVPSDTGAKYVMVMREKRILFQPQLDVNKYDHIINKTADKFKLDSALIK
                     AIIKAESNFNHRAVSPVGAQGLMQLMPKTASQLNVDDSFHPEKNIEGGARYLRYLLNL
                     YKGNLSLALAAYNAGEGAVAKYNYNIPPYRETQNYVRRVLSFYRSFSGN"
     gene            complement(21535..22287)
                     /locus_tag="PLR13_04650"
     CDS             complement(21535..22287)
                     /locus_tag="PLR13_04650"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQC18655.1"
                     /translation="MQLSLENKVALVTGGSRGIGRAIALGLAKSGADVVIASRKIGDL
                     ENVAGEIKSLGRKCLPVATHIGRLEEIKNLVDKAAAEFGRIDILVNNAATNPTMVPAM
                     DIDDRAWDSIMNLNLKGLFFLSQAVARIMKEKGGGRIINVASVAGLSPDILPVYSISK
                     AGVIMATKVMAQQWAIYNIRANAIAPSLTKTKFSEPLWTNQDILNIAISRTPLGRPAE
                     PDDMVGAVIFLASDASDYITGQVLAIDGGMTI"
     gene            complement(22314..22775)
                     /gene="ybeY"
                     /locus_tag="PLR13_04655"
     CDS             complement(22314..22775)
                     /gene="ybeY"
                     /locus_tag="PLR13_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQC18656.1"
                     /translation="MKIQIENRQTLHKIERRKIRSAVSKIFKILECTDRHISILFTDD
                     EQIREINRCYLGRDKATNVISFSMREGEYGHINPQILGDVVISVETARRDAIRAKLML
                     EEEIDFLLIHGILHLLGYNHENTSRKEAKIMYDKERDVFRQMNSRKNMPAQ"
     gene            complement(22732..25092)
                     /locus_tag="PLR13_04660"
     CDS             complement(22732..25092)
                     /locus_tag="PLR13_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQC18657.1"
                     /translation="MKYFESLRIKMTEFFRNYLEKAAPYFGFVRNSLFQRWMIAVVLC
                     LILALIMAPELNFSKPQFRIGMIAPKNIKADFAFLVEDPQAAEEKKIQDAENINPIYN
                     YNSQIADNIQKKIVKAFSLAAERYNNVPEKQQQPDKIMDLQKEKKRLENNLGAYLTPE
                     EFYILNEYRFSEELQNKFSQLIISFYDNRFIASDAFGKSEKQKGVVIRNLRTQISEDI
                     RDTSALLNMNEIDDPLLKTINMIFRNDEKPLKDAAFSITKKIIEPNLAFNKGATEKKK
                     MAIIGDAAATFFQVQKNEMIVREGEKIGYLELAKLNAYFKDDQDNRFTRVAIVLGFFF
                     TAMFLSIVLYLWRTRNWVKTSDRSNLDLLVFGIIALLQILFIKIGIFMSIAINRSFSF
                     IPVDACYYAIPFAVGAMTIAILINRNVALIISALTSFLIGFLFDEKIVFPLFSFLGSI
                     AASYQIVRIRQRSVFLKIGLFLGIINMAAIVCLNLITGNFFNDLPIRLIMGFIGGIVT
                     GILVAGITPLFESLFGFITDIKLLELANLNQPLFQQMIIEAPGTYHHSIIVSSLAESA
                     AEAISANALLTKVSAYYHDIGKLKKPQYFIENQPNSDNRHDKLSPKMSSLIIMSHVKD
                     GCELAAKAKLGRQIINIIRQHHGTSIISFFYDKAKKDKNESLRSLSENDFRYPGPKPQ
                     TKEAGLIMLADVIEASSRTLTNPTPARIKSLVRERIEGIYMDGQLDECELTLSNLNTI
                     AETFTKILTGIFHHRIDYPEAPQKEGNGKKESNENTNRKQANPAQD"
     gene            complement(25095..26093)
                     /locus_tag="PLR13_04665"
     CDS             complement(25095..26093)
                     /locus_tag="PLR13_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQC18658.1"
                     /translation="MIKALKNEDAEKIVFADQHLLKNLCGEHDKNLKFIEKLEPVKVK
                     AWGNSVTISGRNGNVHKIKTMLQQMYALMEKGWHIQSADIDYAHRILTEDVTFELASI
                     FLNTVFISSQKRVITPKSIAQRLYIEYMRNADMVFSIGPAGTGKTYLAMAMAVSCLME
                     RKVQRIILARPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMLDMERATALIHKGL
                     IEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFGSKAVITGDITQVDLPT
                     EKVSGLIEAENILKKSAGIRFVHFSEIDVIRHPLVQDIIKAYAHLTKSKNTNHHNV"
     gene            complement(26261..27505)
                     /locus_tag="PLR13_04670"
     CDS             complement(26261..27505)
                     /locus_tag="PLR13_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HQC18659.1"
                     /translation="MIDEKLFQKISQRIDAYRDGMIELQTALCAHPAVGPENGGDGEL
                     VKANFLKDRLLQMGFKNFQHYDAPDNRVSSGIRPNFVTTIEGKNKNRFVWIITHMDIV
                     PPGELRLWSSDPYKAYVKDGRIYGRGVEDNQQDLVASIFAAKALMDEGVVPENSIKLA
                     FVSDEETSSSKGLFYMIDSTDRLFQDNDWIVVPDSGNAEGSLIEVAEKSMLWICFKTK
                     GRQCHGSTPHLGNNAFVAGSHLVTSLIKLRKMFPDTDPLFDPPQSTFEPTKKEANVGN
                     INTIPGEDVFCMDCRVLPEYDLKDVMNAIQKICRKIEKKFNVRIETSMVQYVQSAKPT
                     PSDAPVVLALQDAIDAVYHVKAFTGGVGAGTVASYVRKKNYPVAVWSKTNQFAHQPDE
                     NCPVENMIGNARVFAYLFLRKS"
     gene            complement(27527..28969)
                     /locus_tag="PLR13_04675"
     CDS             complement(27527..28969)
                     /locus_tag="PLR13_04675"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQC18660.1"
                     /translation="MDKMISKIYPVFLVGVFLFLMMATVRPSSANQADAARPSPDQLQ
                     YEPLFFELPKAHRVVLENGIILYILEDHDLPLVTINAFVKTGSMHDPAGKEGTAELTA
                     QVMRTGGTTRLSSREIDRQFDLIAASASIAMAMESAHVGFSVLTTHLDKGLDLLSQIL
                     IHPVFEQGKLELARQLQLEEIRRIKDDPQKLAMREFSRLIYHQDPRGRFPSSASLTEI
                     KRDDLTAFHSRFFLPNNIMFAVSGDITKEKAVEKIKQYFGGWNTKNSATDFPAPVQQP
                     QTGMYFIEKDIPQSTIISGQFATGKNHPDFHAFTVLDFIVGSGGFPSRIMSTVRNNEG
                     LAYSAGSFYRARPDYGIFGSYAFTKTSSTMKALSLINTVLENIKAVPVAGQELTWAQK
                     SINDGFLFSFATPEQIVRQQMTIEYEKLPPDFLTSYRDKINRVTVQDLHHAAVKYLDQ
                     TKNTVLILGDGKNPDHPLPPGGYTVIAPID"
     gene            complement(28976..30481)
                     /locus_tag="PLR13_04680"
     CDS             complement(28976..30481)
                     /locus_tag="PLR13_04680"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQC18661.1"
                     /translation="MKACKILKAALLPAFIFIFIAAGSGHASDLKEKVLKQKLPNGIT
                     VLMLKRHLSPTVSLYIRYRVGAVDETKGESGAAHFLEHIMFKGTTSIGTNDYQAEKAL
                     MEEIKNAGRRLDAEKRKGQTADPETLANLSAVLKKLQDEHKKYFLPNEIDRLYTENGG
                     LDMNASTGQDVTSYHVSLPANKIELWARIESDRLLHSVFREFYTERDVVMEERRQRVE
                     SDPEGKLYEKFMSAAYTLHPYGRPVLGWTEDLMNLSPDELEKILEKYKDPRNIVIAVV
                     GDIKPEPTLKLIEKYFGGIPPAAEKTESYIPAEPPQTEERRVNVAFDANPSLIIGFRK
                     PNAPAREDYVFDVIETILTRGRTSRLYTTLVIDRQIAQSVSAVNGLPGSRFPNLFALF
                     AQPRHPHSNIELENDILKEIEKLKTQPVSDEELMKAKNHIKMSYLQSLDANADIASIL
                     SYYEVLLGDYRYFADYLDNIDKVTAADIRQIAARYLNSNNRTTAFLNKEGG"
     gene            complement(30468..30704)
                     /locus_tag="PLR13_04685"
     CDS             complement(30468..30704)
                     /locus_tag="PLR13_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18662.1"
                     /translation="MKVLIWIKNVLILLVSLFFLLIGINTLMGSYRINNPMEFLMYFF
                     SASLLILVCIVGILYFIFRLFPQKQEGLNKDESV"
     gene            complement(30701..32068)
                     /locus_tag="PLR13_04690"
     CDS             complement(30701..32068)
                     /locus_tag="PLR13_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18663.1"
                     /translation="MSRKILLINPWIHDFAAYDFWIKPLGLLYLGSLLRKNNHRIDLI
                     DCLDPFHPQMAASGLKKPRRYASGDGKFYRQIIDKPAPLKWVDRSYCRYGILPDVFAA
                     ELKMHRDADMILMTSMMTYWYPGVFEAIRIIKDTLPQIPVVLGGKYATLCYDHASNFS
                     GADYVIAGAGEKQILDLLEKVFSEKTLFVPDENNLDSCPYPAFDLISKTGQLPVITSR
                     GCPYRCSYCSSHILTPGFRRRDPVKVADEIEYWHRKTGVVNFSFYDDALLVNPEAMIV
                     PLLDELQRRNLSCRFHCPNGLHLREITKELAERLYHAGFKTIRFGFETSDLDRQRQTG
                     GKVKNEELRQAVEHLKNAGYKTEDLGIYLLCGTPGQTVREVINSIEFVQECGAKPLLA
                     EYSPIPGTAMWQEAVACSPFDILHEPLYHNNSLLPCGNDDFSFARYTELKKKLKNGSR
                     TGAPS"
     gene            32266..33021
                     /locus_tag="PLR13_04695"
     CDS             32266..33021
                     /locus_tag="PLR13_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HQC18664.1"
                     /translation="MKKTTLTSIFFRSFFIHATLNFRRMQNMGFGMALIPMIRELRLQ
                     KKDAVKILTAHLQMFNTHPYLSAPIIGSIVRLEEDHVAKGENSDTASIRQSLMASYAA
                     IGDIFFWGAFRPFAAIVAVLLVHIGLAMAPVIFLLIYTPAHLWVRLKGFIEGYRKGKT
                     GFEFIRALNLPAAAVRIRWITLMVLTGLMIWLARDGGYWPFGETYGVVAKLIALATVM
                     LCYILIKKGISQVYIVYGAVLAFVIISWTGWIS"
     gene            33018..33287
                     /locus_tag="PLR13_04700"
     CDS             33018..33287
                     /locus_tag="PLR13_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQC18665.1"
                     /translation="MTETKTLRLKNKLGMHARAAASFVKVAQQFQSEIYIEHNGQTVD
                     GTSILDILTVACPYGGELTIKADGADAAAAIEELVKLIENKFGEE"
     gene            33295..35076
                     /gene="ptsP"
                     /locus_tag="PLR13_04705"
     CDS             33295..35076
                     /gene="ptsP"
                     /locus_tag="PLR13_04705"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQC18666.1"
                     /translation="MSPDQTTKTFVLKGVGVSPGIAIGKCYRYDPLDSQISFYKLKDE
                     SLIPEEISRFKNALKESEQQLLKIQKNLKKANVAEPVYVIDVHILILKDKIFINRTIK
                     YIREMSVNAEWALRMTLDHYKEVFEGVEDDYISSRVSDVQYVVQRILRNLSGGKSEIV
                     WETENRGVIIVSHDISPADTAQMKLDKILGFATDSGGRTSHTAIVARSLELPAVVGLD
                     DITRFVHEDDDLIVDGTAGMVVINPYPDMLKRYEEKKRHYDAANDEYLKYGRQPAVSL
                     DQHRIQIGCNIEFIEEIPSAVAHGAENIGLYRTEFLYIYRENLPTEEDHFTNYRQVVT
                     NKNLAWSTIRTFDLGGDKFPNYQKQSKEMNPQMGMRAIRFCLKEVDIFKIQLRAIWRA
                     SALGNIKILFPMISSVDEIREAKKILEEARRELIDEGMKIPEKIDIGAMIEVPSAVVI
                     ADKIAREVDFFSIGTNDLIQYSLAIDRANERLTYLYEPLHPAVLRQIKKVVDAAHKAK
                     IEVSMCGEMAGDPLCVLVLLGMELDELSMNHLAIPRIKKIIRESTLKESKSMLKRALS
                     FNTASEVRSYVQKYMARRFPEDFKTED"
     gene            35120..37741
                     /locus_tag="PLR13_04710"
     CDS             35120..37741
                     /locus_tag="PLR13_04710"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQC18667.1"
                     /translation="MEENNHPVKSNQGIQYYSGLLYDNQNFLQFRLAKTFLSRVSIAD
                     EYVENIKKLSGEGVVVYALNQRSELNSLILYELLQRNDMPVPVYCHGMNMSLAHPLPQ
                     MLKFFWSSLKRRMGRERKAWQGKLAFIEKTIMEKKSIVIHLGESEFIENRSAESAMST
                     LLDIQKKAGFPVFVVPMLVSYGRRREKEDEGLINILFGQTEHTGALRRLITFIRYSRS
                     AFVIPSEPVNLSEYLKTTKKTATDAMVHELRGELIDRIDKEKTAIVGPALKSREELIG
                     MVLSDESLKAFIEKHAQEEKKDKARIKKDARRYLYEIAADYSETFIAIWKKVLTWLWN
                     DIYDGLSIDVEGMAKIRNISKKMPFVIVPCHRSHIDYLLIHYVFYINNIQLPFIAAGN
                     NLSFFPMGYIFRKSGAFFLRRSFRGNKVYSEAFTKYLAILLKEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMVLQAYQEKYCENFAAIPVYIGYDRVIEEKSYLKELSGETKTPENT
                     AEIIKTSKILTKRFGRVYVNIGEPMIMKDYLAQQAKPLEDMSLEERQSLYRKIGYEIV
                     LEINNVSVVTPFALVAAVILSHDRRGISYNDLLYVLNEFYEYLTVKQVKFAETFAHRD
                     KAIIDALNMFVQAGFIEKIEAEKDEVEEMQEVVYSLKEERRLNLEYYKNNILHFFVPL
                     CFVATSIIKNNEDLISLNRIQGDYGFLKKLLWNEFIFDKHKDEAEEVNEVLSYLYDRK
                     IIVSVEQDGQTLIEIKGRGNKRLKPLADLIHNYLESFWIVMRSCLYLKKNPLPKKEWL
                     KKIMALGDRMYRKGEVLRKEALSQANYQNVIIYLEDADLIKSFRDEKIDKKDVLYTLA
                     ENRAEMEVLRRRLFRFL"
     gene            complement(37854..39167)
                     /gene="eno"
                     /locus_tag="PLR13_04715"
     CDS             complement(37854..39167)
                     /gene="eno"
                     /locus_tag="PLR13_04715"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQC18668.1"
                     /translation="MPEIIHIHAREILDSRGNPTVETEVTTLSGITARAAVPSGASTG
                     EHEMLELRDGNKKRYNGKGVEKAVDNILHKIGPEIIGMDCRRQRDIDFTMIEMDGTAN
                     KGKLGANAILAVSMACAKAGAEASGLPLYRYLGGVNSHVLPVPQSNILNGGQHADNNV
                     DIQEFMILPVGAPNFKEGIRMNAEVFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEA
                     LACIMTAIDKANYKAGRDIMIALDCAASSFYEKGKYILAAEAKPRKSSEELVKFYAEL
                     VAKYPIISIEDGLAEDDWSGWKVMTEELGGTIQIVGDDLFVTNVKRLNKGIDLGIANS
                     ILIKLNQIGTLTETLETMSRAKECGYTCVVSHRSGETEDTFMADIAVATNCGQIKSGS
                     LSRTDRLAKYNQLIRIEEELGDAAVYRGKETFYNMKQPAKSGRKR"
     gene            39314..40072
                     /locus_tag="PLR13_04720"
     CDS             39314..40072
                     /locus_tag="PLR13_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18669.1"
                     /translation="MNQDTKDRNTKLFLHMKKWLEKAVLDYKMIQKGDRLLIGVSGGA
                     DSLALLDLLDSPMLFIPDFSFVAVNIDMGFDPAYKKYDRLEKYFQKNNYPYVMEKTDI
                     GTVAHSDVNKKNPCFLCSRLRRKRIFEIADAEGCNKIAFAHHRDDIIETLLINMFYGR
                     EISTMMPNQSIFGGKLHIIRPLVYLSEALVKKYSRERRFPVLKNDCPTSKTSKRVYIK
                     NLLNKLERDNKEIRDNIYKAMSHVKPDYLLPQKK"
     gene            40162..40638
                     /gene="smpB"
                     /locus_tag="PLR13_04725"
     CDS             40162..40638
                     /gene="smpB"
                     /locus_tag="PLR13_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQC18670.1"
                     /translation="MAKEKTAQKLIASNKMARVNYEIGDIYEAGLVLTGTEVKSLRAG
                     KANLKDSYAVVKDDEVYIKEMHISPYDFGNRSNHNPLRTRKLLLHKREIKKLYGKSRE
                     KGLSLIPLKLYFKNGKVKVEIGIGKGKKLYDRRQDIKQREERRSLSRDFKSRQIKV"
BASE COUNT        10724 a         9268 c        10614 g        10081 t
ORIGIN      
        1 tgaaaagaga gtataaatcc ggtctgtgct tgacatttac tgtattttat ttaagaatac
       61 aggcatacaa agattaatag aatgtaagga aaatcatgga aaaaatcatt aaaattcatg
      121 tcaagaaatt gccggaaggg gtttatttgg caacgtccaa agatgttccc ggtttggttg
      181 ctcagggacg aaccgccacg gaaacactgg agattgcccg tgatgttgcc aaaaaattac
      241 tggaagccag aaaagaacgc cgtggcaaga taaaaatatc cgagattaaa gactcatttg
      301 attatccgat gattgtgagc gcgtaacgtg ggacgattgg cgggtttccg gtatcgtgaa
      361 atcgtaaagc gtcttaaatc gtttggcttt gcttttgaca ggcaagcggc gggaagtcat
      421 gaaatctggt acaattcatt gaacaatctt tatacaacca tacccaatca tcccggagat
      481 ataccggaag gaacgctgag agccatatta aagcaagctg gtattacccc gaatgatttc
      541 ttaaagaaca aataaggcac atttcccctt ttgatgcagg gcgagatcta acctaaatac
      601 cctaaagggc actatagggt catattgtcc gatagcgccg acttgccata tcggactgat
      661 cgggggatca gtctctacct ttattgtgag actcaaattt caaaaacgta gtgcattgaa
      721 atttgtaagt cgaacaatcc cgcgcagcgg agggtttaat accccgcagc ttgctgcgga
      781 atagtttcca aagcttgctt tggggttcat acccgtgatt cctaaacatg aattctgctt
      841 aatatttgac tttttcagtt tatatgctat cgtccgtcac acattgttac aacgaagcaa
      901 ttcttagcag gagggtcttg aattgaacgt ttttgtcaaa aaaggaatgc ttgcggaaac
      961 caaaagccag gcagtgattc tgacgttgtt tgaagacggc aagaaattat cgggcattgc
     1021 gctggagatt gataaaaaaa gcggcggtct tatcagcaaa ttaatggccg acggtgattt
     1081 tgaaggcaag gcgtcgcagg tagccgttgc ttatccctgc gggaatcttc cgtcgaagag
     1141 aatcgctctg gtcggtttgg gaaagcaaaa ggaattcaat ctcgaaaaat tgcgcgcggc
     1201 atttgccaaa gtgatgcgtc atcttcgcgg gatgaatgtc aaggaagcgt cgacgtccgt
     1261 gaatttgaat cttattcccg gcaagaaaga tcagatcgtc caggcagcgg tggagggggc
     1321 tttgctcggg ctgtatcaat ataccccgta taaaaccgtc ggacgggaag aattcaagga
     1381 gatggatgaa ttcaatatca tcgccgatgc gaaagagtat gccgtgattg agccatctgt
     1441 gaaaaaagcc aaaattattg ccgacgcggt ttgctttgtg cgcgatatga tttctgcgcc
     1501 ctccaacgaa atgacgcctt cgatcatggc acaaaaagca caggaagtgg ccaagagaaa
     1561 aaatgtgacg tgtacggttc tggatagaaa gaagatgcag gaattgggta tgaatgctct
     1621 gctggcggtg gcatccggaa gccaagaaga accgaagttt attattctgg aatattccgg
     1681 cggcagaaaa aaagacgctc ccgttgtgct ggtgggcaaa ggactgacgt tcgacagcgg
     1741 gggaatctcc ctcaaaccgt cagataaaat ggaggagatg aagacggata tgtcgggcgg
     1801 cgccgcggtg atgggagcga ttatggcggc tgcggatatg cagattcctc tcaatgttgt
     1861 tgcattgatt cctgcgtcgg acaatatgcc gagcggatcc gcgttcaagc cgggcgatat
     1921 tttaaaatcg tattccggca agaccataga aattctcagt acggacgccg agggacggct
     1981 gcttctggcc gatgcgctgg cctatgcttc taagttcaaa ccggaagccg tcatagacct
     2041 cgccacgctg acgggcgcct gtgtgatcgc gctgggcgac gatgtcatcg gaatgctggg
     2101 aacgggtgaa aagttgaaaa aagaaatcaa tcgcgccgcg caagacaccg gagagcttgt
     2161 ttgggaactg ccgctgtggg agcaatacca tgagctgatc aagagcgata tcgccgatta
     2221 caagaatgcc ggcaaccgtg ccgcgggcac cattacggcc gccgcatttt tgagcaaatt
     2281 tgtcggcgat tatccctggg cgcatctgga cattgccggg ccggcctggg cggacaagga
     2341 taaaccgtat attcccaaag gcgcgtcagg gatcggtgtc cggttactgg tagaatttct
     2401 gcgcaaccgt tcctgatatc agtctatgcc gccactgctg caaataaaag atctcagaac
     2461 cgtgtttgat accgttcatg gccgcataaa agccgtggac ggggtatctc tggacatcgc
     2521 gtccggggaa accctgggcg tggtcggcga atccggctgc ggcaaaacaa tgctttccct
     2581 ttccattatg cgtctcattc ctcccaacgg caaaattatc aacggtgaaa ttctattttc
     2641 gggcgtggat ttgctgaaat tatccgaaga agacatgcgc gcccgccgcg gcaaggatat
     2701 tgccatgatc tttcaggaac cgatgacgtc gctgaatccg gttttccgcg tcggcgatca
     2761 gatagccgag gcgatccgtc tgcatcagca gctgccggcg aaacaggcca tggcgttgag
     2821 cgtggagctt ttgcgagaag tgggaattgc agagccgcag aaacgggcgc gggattatcc
     2881 gcacaatctc agcggcggca tgcgtcagcg cgtcatgatc gccatagcga tgtcctgcca
     2941 tccgcaactg cttctggcgg acgagccgac cacggcgctg gatgtcacca ttcaggcgca
     3001 gattctggat ctgatttcca atctgaaaca gaaaaataat atggcggtgc ttttcattac
     3061 gcacgatctg ggcgtcgtcg ctcaggcggc cgagaaagtg gctgtcatgt acgcgggaaa
     3121 aattgtcgaa tattccaccg tggaaaatct ctttgcaaag cccttgcatc cctatacgca
     3181 ggggctgctg gaatcgatcc cggcgagatg ttccgatgca aaggaaaaac aggcgtatct
     3241 aaaaacgatt cccggctccg ttcccgatct ctatcgtatg gcccggagtt gccgtttcca
     3301 tgaccggtgc ccctttgctt ttgctgaatg cgaattgcag gatcccccat tgcttgccgt
     3361 ggaagacggt catctggttt catgctggaa atacggatcg gaaaaaaata attaatgaaa
     3421 agagcaaata atgtccagcg ttcttgtcga cattcagaag gtagataaaa aatacacgtt
     3481 aggcggcaat gtgtttggcg ctcaaaagcg aatcattcac gccgtcaaga acgtcgattt
     3541 gcagattatt cggggggaaa cgctggggtt ggtcggcgaa tcgggttgcg gtaaatcaac
     3601 gctggcccgt ctgatcacca ggctggagaa tcccacatcg ggcgtcatcg catataaagg
     3661 tgaaaatatt tttacgttca cgggcgagtc tttaaagacc tatcgccgca gcgtgcagat
     3721 catttttcag gatacgtatt cctcactgaa tccgcgaaag tcggctttaa gcatgatccg
     3781 ggagcctttg accattcatc gcctgggaag caaaaaagag cgcgaggaaa cggcgctgtc
     3841 gattatggcg aaagtcgggt tgaaaaagga acaggcccat cgctacccgc atgaattcag
     3901 cggcgggcag cggcagcgta tcggcattgc ccgtgcgctt gtattgaatc cggaattgat
     3961 tattgccgat gaaccggttt cctcgctgga tgtttccatt caggcgcaga ttctgaatct
     4021 gcttcgggat ttgaaaaacg attttcattt gacgtacctt tttgtttccc atgatctcaa
     4081 cgtcatccgg tatatttccg accgggtggc cgtgatgtac ctggggaaga ttgtcgaact
     4141 cgcgggcggc gaagatattt acaaccgtcc gctgcatcct tatacacgca tgctgctttc
     4201 ggccgtgccg tcctgcgacc cgggtaaaag aaaaacgtcc attgccataa agggcgaagc
     4261 gagggagacg gataacgaag ggtgcgcttt ccggaaccgc tgtccctaca gaatagacgt
     4321 ctgcgataag acggatcctg ctctggacgc cataaacggc caaagctttt gtgcctgcca
     4381 cagggccggt gcaatttaaa atagcctttg tttcctttct gtcatttcgt gtatcatggc
     4441 ggaaagttca atttcaaaaa ggtctccgtg tgttatgcgg gaagaaaaga tcctgataaa
     4501 gaataatgct ctggtgattg aagccttgct gcatcgggca tcgggcgaac gaggtgcggt
     4561 gatctgccac ccgcattcgc tgatgggcgg ttcgatgtat aataatgtcg ttgaagcgct
     4621 gcaggaggcg tttgcatcgg aagggatttc caccttgaga ttcaactttc ggggcgtggg
     4681 ctccagcacc gggtcgtata gcgaaggcgt gggagagaag gaagatattt gggctgccgg
     4741 caactatctg aaaacgtcag gtctgcgcga gatatttttt gcggggtatt cgttcggagc
     4801 ctgggtcggt gccggggtga ttgcgcaacc ggatcatcct ttttgcagca gcgtctttgt
     4861 agcgcctccg atagattatt ttgaatttga ctggccgcct ttaaaaaata aaattgattt
     4921 aatgatctgc ggcgatgccg attctttctg tccgtgggag cctttgaagg atgcgtcaaa
     4981 aaaaataaat gcgttgctgg aagtcattgg cggcgcagat cattttttta tggggaaaga
     5041 aaaaaaactt gttattcttt tgcgggatta tctggctaaa aaaacataaa ttaaataaaa
     5101 aaacttgatt tcaaaaaaaa aagatgataa tatttcatac aacgcataaa aaaccgaata
     5161 taagaaacac gtcaaagata ggatgagaac ggtttttttt aggaagttgt tagtgttaag
     5221 ttgtataata aataagacaa ccgcggagga ttcttaaaaa atatagaatt cttgcggttt
     5281 tttttgtttc ctgtacaaac attaaaacag gaaatcattt tttaaaggag ggttttagct
     5341 ttggcagaag gaaaagtaaa gtggttcaac gagaagaagg ggtttggttt tattgaaaat
     5401 gatgaaggtg gtgacgtgtt tgtacattat agtgcgatta ccggaacggg atttaagacc
     5461 ctttatgaag gtcagaaaat caagttcgat attgaacagg ggaataaagg tcccagcgca
     5521 acaaatgtta cgccactgta atactcgcaa ttgatttatt ctaactgaaa aaatgccccg
     5581 tgatgaacgg ggtatttttt ttcaacgatt ccggatctta agcagccgca ttggatcaca
     5641 ggaccgggcg tgaagtatgc cgtcttgtat atcaaaaaat atttaacggg tttcaagttt
     5701 ccgaagctct ttttctcccc agtaaataac aagaaataat tcaatgaagc cggccagggg
     5761 caatgccatc caggctccgg tgattcccca gaataaaggc aggataatca gtaacggaac
     5821 aaagaccaga atatcccgga tgaaaagaat aatcatggcc agcgtccctt tgccaagtcc
     5881 aatgaacatg ttgacgtaaa tgatgatggg agcggaaaag atcagggctg cgacatggat
     5941 gcgtaacgcc ggtatggcaa tggcggttaa atggggatcg ctggaaaaaa aagtgatgat
     6001 ctgggaagaa aacaattcca ggatgatcac ggaaaccgcg gaaaaaacaa cagagacttt
     6061 caaggcgacg gagaccgatt cccgtaatcg ttgaaagagc ctcgcgccca ggttatatcc
     6121 gacaatcggc attaatcccg cgccgacgcc gaacagtacc atggtggcca ggccggtaac
     6181 ccggaaacaa atgccgaagg ccgcgatggc ctgaaaaccg aaattgccga gaacatgatt
     6241 gtagatcacc agaacaatgc tgatgacaaa attggtgacc atcgagggga aaccggttga
     6301 ataaatggac ttgatgatct gcagatcggg acgggtgtat ttcagtctga aatcatactt
     6361 ggatgatttc aattgcaaaa aataaaggga caagataaac atgatcaagt gcgcgatgat
     6421 accggccagc gcagcgcctc ggattcccat gcgtggaaag gggccgatgc cgaaaatcag
     6481 aaaaggatcg aggatcgcgc tggaaatcag ggcaatcagg acaacgttca tggataaaag
     6541 aggacggcct tcgacacgca gcatgatggc cgtcatcatg gaaaaaaaga gaaaaggcga
     6601 gctgtaaacg gccagaaaaa gataatcatg actcagaggt aatatttcgt cggacgcgcc
     6661 gaaaaagacg agcatggaat ccccgaaaag aagcacgaaa agaatgcttc cgaagccgaa
     6721 cagcatggaa agaaaaaatg tctgcccggc ggttttctgt gcttccagat ttttttcggc
     6781 gccaaacatc cttgcggaaa atgaaccggc tccgatgccg gttccgacgc ctatcgcggc
     6841 ggcaagcagt tgaatgggaa agcacacggt cagtgcggca agtgcctgag agctgaaacg
     6901 ggcaagccag aaggtgtcga tgaaattgta aagagacatg gctgtcatcg caatgatcga
     6961 cggccagctc atttttaaaa tcagcgggag gattgcctct tttcctaaat ccagtctctt
     7021 attcattatg ggtcatttca tttgaatgcg tttagttgct agcatataaa gaattagaat
     7081 atcaagataa aatatggcaa aaaatcttat tgttatcctc ggtccaacgg catccggcaa
     7141 aacccgcctg gcggcacaac tggcctgtga ttttcaggga gaagtgattt ccgcggattc
     7201 ccgccaggtg tacagaacca tgaacatcgg caccggaaaa gatttaaagg aatatgtgat
     7261 tgacggccgg cagattcctt atcatctgat tgatattgaa gacccggaaa acgaatttaa
     7321 tgtattcgag tttcagaaac gattttatgc ggtgttcagc gctatccgcc aaagaaagaa
     7381 actgccggtc cttacgggcg gaacagggct gtatctggaa gcggttcttt gcgcatatga
     7441 tatgcccgaa gcgccgatca atgaagaaat caggcgtgaa ttatcagaga aatcgatcga
     7501 agagcttcag gatcttctgt gccgtttgaa gccgcatctc cataacagaa cggatcttga
     7561 tgataaaaaa cgactgctcc gggctgtcga aattgaaaaa gcgcggattc gcaataatca
     7621 ggatttgagg gacaggcctg aaattacagc ggctgtcttc ggggtgcgat gggaacggtc
     7681 ggtcctcaga aaccgcatca caaaacggct tgaagagcga ctggaaaacg ggatgattga
     7741 agaagttgcc gatttgcatg cggccggttt gagctggctt aggctggaaa gtttcggcct
     7801 ggaataccgt tttatttcgg aatatctgca gcagcggctt acgtttgatg aaatgaaaaa
     7861 tcggttaaac atcgcgattc atcagtttgc caaacgacag gagacatggt tccggcgcat
     7921 ggagagaaaa ggtgttcaaa ttaactggat cgataacggc gactacttgc ttctggcaga
     7981 aaaggttgcc aagcatttaa aatgaagaag cgttccgcat tcgatgatta cctgcacacg
     8041 tactccgtcg gcgaaaaatg gaagttatgt gccgtcccgg atcaaaattt ctctcaggtt
     8101 gtcgtcattc cggcgtacgc cgagaaagac ctgcttttcc gcacgctggc ttctttagcc
     8161 gtcaatgtgc ctgatgcgct ggagaattct tttatcctgt gcgttgtcaa tcacaaagaa
     8221 gattcccccg cttccgtcat ggaaaacaat aaaataacca tggcgtattt gaacgctctg
     8281 atcagacgga agtctctgaa aacgtttcag cttaccaaag acgtcacggc tcttttgcag
     8341 tgcatatcgg attcaaaaat gaagctggga tatattgacg ctgcctcgcg gggatatgaa
     8401 ataccgcgca atacaggcgg ggtcggcatg gccaggaaaa taggcatgga catggctttg
     8461 cgccttttgg agaaaaatgc gccgcaaaac ggcgcgatcg tcagcctgga tgccgatacc
     8521 ctggttgaac cgaattatct ttccgtcgtc cgggattatt tcagaaagaa tgttaaaacc
     8581 gcgattatgg cctatgcaca ccagatgccg gaaaatcccg cggaacaggc ggccatttgc
     8641 tgctatgaaa ttttcttgcg ttactgggtt ctggggctgc ggtatgcaaa atctccctgg
     8701 gctttccatt ccatcgggtc gaccatggcg gttgccccgc aatcgtatct ggccgtaagg
     8761 gggatgaaca aaagggcggc gggagaggat ttctactttc tcaacaagct ggcaaaagtc
     8821 ggccgcatcg actacatcag ggaaacgtgt gttcatccgt cggcgcgcgt atcaacgcgg
     8881 gttcccttcg gaacaggcaa acgcatcgag cgctttcttg cgggagacgc cgcaaaagaa
     8941 tattacctgt acgatccgca gatattttca atattgtccg gctggctcag tcttgtacat
     9001 cggatgattg accgggacgt ggatgacatc ttgatggagg ctaaacggat tcatcccgcc
     9061 ttaaaggcct ttctggaaga ggcccggttt cctctgcgct ggtccgccat acggcgcaac
     9121 acaaaagatc aagagactct tttgaaccat tttcatgtct ggttcgacgg gttcagaacg
     9181 ttaaaaatga tcaattattt ttcccgggtg gcttatcctc cggtggatat gttttcagca
     9241 ttgaagcgga ttttcaacct gttagatctg cctgctttga gtccggaagt cgcagaagat
     9301 attccttctt tgggcgaaca aatggaaatt ctgcgctttc tgagaacaat cacctgaacg
     9361 tcatttagta ccggaaaatt ctcttgacgg atacggataa tttacatata acacgtgaaa
     9421 atctttaaaa tttgcaaaat tttcgggagg aaattcatga aaacttggat cggctcaaaa
     9481 tggcccataa tttttgtttt gtttgttttt gtcatggctg ccggttgttc ggatgccaaa
     9541 aaaggtgcgg agaacgcagt tggtgcaaac agtgttgaaa gtttggatgc aggacagccc
     9601 tcgacggtta ctgatgcggc tgcccaaaac attccggcag gcgatctcgc cgtcacggtt
     9661 gacggaaaac atatgaccaa ggccgaactg gataaggcgg tcaaagaaaa attcgagctt
     9721 atcaaaaaac aggtgccggc cgacaaacaa aaagcattca gggaaaatct cagaaagcag
     9781 ttggtggacg tctttgtcat gaagacactt ctggccaacg aagtggagaa aagaaaaatc
     9841 gtagctactg atcaggatgt caaagcggcc atgaatcaaa tcaaagccag ccttccgccg
     9901 aataaaaatg tcgacgactt tcttaaggaa aataaaattt ccaaagaaga cattattctg
     9961 gccgtcaaga tcgataaatt cagaaatgcg gaagggggca aggcgctgaa acccacggaa
    10021 aaagacatca acaagtttta taatgacaat cgtgaaaaac tatttgtcga accggaaagc
    10081 gtgcatgttc gccacattct ggtatctgtc ggcaaggacg acagcgagaa agtcaaggcg
    10141 gagaaaaaag caaaaattga cgatctgcgc aaacaattgg tcggcggtgc aaattttgcg
    10201 gaaatcgccg gtcagcattc cgattgcccc agcaagcaag ccgggggcga tttgaatttt
    10261 atcaggagag gccaaatggt gaaagcgttc gaggatgccg cttttgcgca ggagaaaaat
    10321 gccatcggtc cggtcatcaa gacggaatac ggttaccaca tcgttcaggt gctggaccgc
    10381 agaccggcga agaaaattgc cttaagcgaa gtcaaaagca aaattgccga ttatctggaa
    10441 agacagaaaa aaatggaagc cttcaacaac ctgatggcca agctgaaggc gcaggccaaa
    10501 ataaccgttt attaaacatc attacaacgt tttacagagt tacttgaaac tgtgttccgt
    10561 atcggggaag ttgctgttct tgacttcccc gatatatgtt ttgaccgctt tcttgatttc
    10621 cagattgagg ttggcgtatt ttttgacaaa tttcggcgtg aatttttcct gcatgcccag
    10681 catatcgtga acgaccagca cctgtccgtc gcaataaaca ccggcgccga tgccgattgt
    10741 cggaatagac agggcgctgg tgatttcagc agccagtttt tccggcacgc attccagcac
    10801 aacggcaaac gcgcccgatt cttccagaat cctggcatcc tgcatgattg tttcagcggc
    10861 gtcgtctttt ttgccctgga ctttatatcc tcccaactgg tgaaccgatt gcggtgtcag
    10921 acccagatgc gccatgacgg gaattcccgc atacgtcatt ttatgcacga tgtccgcaaa
    10981 ttcccggccg ccttccagtt tcacagcctg ggcatcggct tccttcagga accgtcccgc
    11041 attggcgagc gccgcctcag gagatacctg atacgataaa aaaggcatat ccgcgacgac
    11101 catcgccttc ttaaccgcac gcgcaacagc tttggtatgg tgaagcatgt cgtccatcgt
    11161 gaccggcaag gttgaatcgt agccgagcat gacgttcccc agagaatcgc cgaccagaag
    11221 catgtcaata tcgctttcat ccagaataga ggccatggcg taatcgtaag ccgtcagcat
    11281 cgcaattttt tctcccgttg ttttcatctt tctgatatca gcggttgttt tacgcgttat
    11341 tttactatga gtgctcatgc tttcctcctt ttaaaagggc gttgattttt cttgcctgtc
    11401 cggcgttgag ggttcctttc tgttgcgcaa tcttgacggt tatcaatccc agagaagcat
    11461 ataacgaaga ataccgggga agatttttat caatggtccg cagatgtttc cggatggtgc
    11521 cgtaatctcc gcgtgcgatg ggaccggtca gggaggatat ggaaccggat ttctcgatgt
    11581 ttttcaaact gccgtatacc aggggcaagt aagctctttg tgcgtctttt tccctgatgc
    11641 ctatggatcg gtaaatcgat tccacaatgt tcatcagcga tacaagataa ttggaagcga
    11701 aacaggcggc cgcgtgatag agcggtttct gatcgtttga aatgaccatg gggatgccgc
    11761 ccagtttatg aatgatatgt ttggccggaa ttaaggaata tctgtcggcg gtaacgccga
    11821 agtagcttcc gggaatgttt ttgatggcct ggtcgatgga tgaaaagctt tgtaacggat
    11881 gaatgcttgc cacaaaagca ccggcttttt ttgacgattc cagaaggtca aggccgccgg
    11941 cgccgctcat gtgaaacaca tattttcccg cgatggatga cgcggaggcg atttcccggc
    12001 aggccgaggc aatatgatca tccggggttg taatcagaat agaatcggcg tgtccaacgg
    12061 catccggaat cgttttgcac ggttttcctc cggtatagga aacggctctt ttaagggcgg
    12121 tcgaagattt gtcgcagatc gaaacgacgt tgtaaccgga ttttttcaac agagagccta
    12181 tcgcggtgcc gaccataccc gtgccgatga tggcaaagtt atttaactct tttttcttca
    12241 tgtgatcacg catcgctgtg catctgacta actgaaacca tatccctgcc gcgtcagagg
    12301 cagggacaaa catcgctgcg ctcgttttca gcaagtctca aatcccacgt cgctacgctc
    12361 ctgggataat tcgctctaag attttccgtg cactacgttt cggaaaatca aggctcatga
    12421 cgacttacaa atttcaatgc gctatgcttt tgaaatttga gtctcaaatc ccacgtcgct
    12481 acgctcctgg gataattcgc tctaagattt tccgnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnggataa ttcgctctaa gattttccgt gcactacgtt tcggaaaatc
    12661 aaggctcatt aaaaaaggcc ttccgcaagg gaagaccttt atccgacatt tcagttcaat
    12721 accgtctcag tcgataagag agatccaagc ggttagaact tatctaacac cgacccaaag
    12781 cttagtcaaa cactttttca taaagaatct tgtgtttacg gacgcacttg gttatataac
    12841 ttaacaaatg cgaagaggtt catgatgaag aaaatagatc tgatgatatt cgatttggac
    12901 ggtaccattg cctcgacggg cgatgacctg gttttgtgca tcaaccatac cttaaaaacc
    12961 ctgaacctca gggaaagaac gaaaaaggac attatcggct ttgtcggcga cggaatcagc
    13021 aaattaatgg agcgggcttt gggaagccgc tatcgcgaat accaggaaga ggccatgcgg
    13081 atattttccg cgtattacgc ggagcatctt ctggataata cgaaactgta tccccatgtg
    13141 gaagaggttt tacggaattt tgagaacaaa ccgaaagtcg ttctgaccaa caaacggtat
    13201 ccttatgcgt tgatgattgt cagagggctg aagatagaaa aatatttcac ggaagtcatc
    13261 ggcgccgatt caactccttt tttaaaaccg gatcgtcgta ttatcgatag cgttctggaa
    13321 aaacatccgg ccgaacggca cagagccctc atcgttggcg acggcgtcaa cgatattgcg
    13381 gtcgccagaa attccggaat aatgagttgc gcttttttaa acggattggg ccgtcgcgac
    13441 gatttgctca aagcgaaggc cgattattac tgtgaagaca ttcgggaagt gaatcggctt
    13501 tttagatagt tatgatcaaa aaagtgaaac agaccatcga gaagtttgat ctgctgaaaa
    13561 aaggagagcg ggttgtcgtt gccgtttccg gcggacctga ttcgaccgct tgcctgaccg
    13621 ttctggcttc gatcgccggg gacatggacc tggaactcat cgttgcgcat ttcaatcacg
    13681 gcttgagagg aaaggaatcc gaccgggatg aacagtttgc ccgccgcctg gctaaaaagc
    13741 tggggcttgt tttctatgcg ggaaaactga ataaaaagat tcgtgtcaaa ggcatttcgc
    13801 cggaagattt ttaccggcgt cagcgatatg ccttcctgga gaaaacggcg gcaaagcacc
    13861 gggcgcagaa aattgcgctg gggcagcatt taaatgatca ggcggaaacg gttttgctga
    13921 atttattgcg gggcagcggt ttggaagggc tcaaagggtt tctgcccatc agggaggggc
    13981 ggatcattcg tccgctgatt gaagtatccg cacaggaaat catttctttt ctggagcagt
    14041 cgggcatggc ctattgccgg gatagttcca atgaaagcag tcaatatcta cgcaatcaga
    14101 tccgggcaga gctgattcct tttttaaaag ggaaatacaa tccgcgaatc gaagagaatc
    14161 tggcgcaaat ggctgaaatt cttcgccggg aagatgattt tatccgtcag catgcggccg
    14221 cagccgcaaa aaaaaccatg cagcagaaaa aggccggttc tgtggcgttg aacatccatg
    14281 cggttaaaaa aattcccgaa gccatccggt ggcgattgct gaagatgctt ctggaggatc
    14341 tgacgccttc aggcgacggc atatcgtttc ttcatgtgaa tgcggtgaat gatctgacgc
    14401 agaaaaacgc atccggaaaa aagatcacgc ttccggccgg aatcgaagcg agacgcgaat
    14461 acgatcagtt aatcctggag aagaaaagga cgcgtccaaa aaaaaatcaa tacgcctatg
    14521 tgatgagcgt tccgggaacg gtccatgtga aagagagggg gctgacgatt aaggcgacac
    14581 tggttaaaaa aattaagacg gacttatccc gcatgaaaaa tacggcgtgt ctggattgga
    14641 gtaaaatcgc tcccccgctg gtggtcagaa acagacgcga cggagactgg atccagcctt
    14701 tggggatgcg cggccggcag aaaattaaaa aatatttcat cgatcataag attcccgttg
    14761 ctcaaagaga cgacctcctg ctgctggttg accgtctttc cgtcgtctat attgagcata
    14821 ggcatatcag tgaccgtgtc aagatttcac cggcaacaaa atcggtcctg aaactggcga
    14881 tcacgcctga ccggcctgtc gggaaaggtt caaagccgcc cggataaaat ccatagaaat
    14941 tatttgagat aaattcaata ttattataag agtaaaataa ctgttctaaa ggagattaaa
    15001 tatattgaat cagtttcaga aaaatatagc gcttgtcctg accgtcctgc tggttttcct
    15061 gcttgtctgg cagctttaca atcagcctaa aacgggaacc aaggaaatca tatacagtga
    15121 gatgttaacc ctgttggaca agggagagat cagcgaagtc accattcagg gcgatcatat
    15181 caacggaaaa ttgaaaaaca acagtctttt taagtcgttt gcgcccaaag acgaccaact
    15241 ggttgaaaag ttcagcggga aaggcgtcaa gataactgcc aaaccggtgg aggaatccgc
    15301 ctggctgacc attttgattt catgggcgcc gatgattctg ctgatcggta tctggatctt
    15361 ttttatgcgt cagatgcagg ccgggggcgg aaaggccatg gctttcggta aaagcaaggc
    15421 gcgcctgatc accgataaat ccaagcgggt tacgtttgcc gatgtggccg gaattgatga
    15481 ggcgaaatcc gaattggaag aagtgattga tttcctgaag gatcccaaaa aatacaccaa
    15541 gctcggcggc aggattccca agggattgtt gctggtcggc cctcccggaa cgggaaaaac
    15601 gcttctggcc cgggccattg ccggagaggc ggaagtgccg tttttgacca ttagcggctc
    15661 ggattttgtt gaaatgtttg ttggcgtcgg cgcctcacgt gttcgcgatt tgttcagtca
    15721 ggccaaaaaa aacgctccct gtattatttt tatcgatgaa atcgacgcgg tcggccgcca
    15781 tcgcggcgcc ggtctgggag gcggccacga cgaaagggaa cagacgctca accagctgct
    15841 ggtggaaatg gacggcttcg aatccaatga aggcgttatc ttgatttcag ccaccaaccg
    15901 ccccgatgtc ctcgatccgg cgttgctgcg gccgggacgt tttgaccggc aggttgtcgt
    15961 gccgctgccc gacgtcagag gccgggaaaa aatcttcgag gtgcacgccc gcaagacccc
    16021 gattgcggga gatgtggatt ttgccgttct ggcccgggga acgcccggca cgtccggagc
    16081 cgatattgaa aatctgatca acgaagcggt gctcaacgcc gcccgggcca ataaagaaaa
    16141 ggtgaacatg agcgatttcg agtttgccaa agacaagatc ctgatgggct cggaaagaaa
    16201 aaccatggtg atcagcgatg aagagaaacg caatacctcg tatcatgaga tcggccatac
    16261 gctggtcgcc cgtctgattc cgggaacgga tcccattcat aaagtgacca tcattccccg
    16321 cggtcgcgcg ctgggactga cccagcagct gcccgtggat gaaaagcata cctatccgag
    16381 acggtatctg gaaaacagca tttccatttt gctgggcggc agagctgccg aggaactggt
    16441 cttgaaagac ttcaccaccg gcgccggcaa cgacattgaa cgcgcgacaa atttggcccg
    16501 gaaaatggtc tgtgaatggg gcatgagtga caaaatgggg ccgttgagtt acggcaaaaa
    16561 agaagagcag atctttctgg gacgggaatt tgccacgcac aaggattata gcgaagaaac
    16621 ggcaaaaaac atcgatgcgg aagtcatggc cattgtagcg cgcaattatg aacgatcaaa
    16681 acagcttctc accgatcata ttgatcttct ccacaagata tcgggtgaac ttctggaaaa
    16741 agaagtttta agcggcgcgg aaatagacac cctgatcggc gatgcgttac ccaaggcaca
    16801 caggagcgag ccgcagactt cagtctgatc actatatcac ttcaaccatc cgttcataat
    16861 attgggaatc ttgaaatttg gtgatttctg gtgaagataa ttgatattca aagcacacaa
    16921 gaggcggctg cgctgttcag gcggatcggg gtggatgcct acggcatcgg cgcgatggcg
    16981 cccaaagcgg tcaatctgaa tatcctgctg gagaaacagc cgtgtaaaat tgccaatatc
    17041 ctgaagcagg aaatgctggc tctcggcggt gatgcggcgg ttgccagagg aagtgtcgcc
    17101 tgcagcgtgg aaacgaccga tgttttaatg atggggacgc tgaaacagct ctcccggctg
    17161 gcggcgaaga tagcccggca gccttttggt ctggatagca tcgcccggga tattttgcgg
    17221 attctgaaaa acgccgggca gaaacggttt atcttaaaaa catcccggag gaaaatcacg
    17281 ctgggtgaca gaacccggat tatgggaatt ctgaacgtca ccccggattc gttttccgac
    17341 ggcgggacct ttctatcagc ggaaagggcg gttgaatatg gattgcagat ggaggcggaa
    17401 ggagcggata tccttgatgt cggcggagaa tcaacgcgtc ccggttctgc cgcagtaccg
    17461 gttcatgtcg agctcaaaag agtccttcct gtgatcaaag cgttgagccg gaaaataaag
    17521 attcccctct ccatcgacac gaaaaaagcg cgtgttgccc gggaagctgt cgaggccggg
    17581 gcggaaatca tcaacgacat cagcgccctg aacggcgatc gtaaaatggc cgggacggct
    17641 gcggaaacgg gcgccgctct tgtgttgatg cacatgcggg gcaatccgga aaatatgcag
    17701 aagggaaatc tggcctatga tgatctgctg ggagacatca cggattattt gagcgccagt
    17761 atcgggaaag ccatccgggc aggggtgagc aaagacgcaa tggtgattga tccgggaatc
    17821 ggtttcggca aaacggttgc ggataactac agaatcatca ggaggcttgc agacctgaga
    17881 actctgggga tgccgatact ggttggacca tcgcgcaagt cctttatcgg gaatgtgaca
    17941 ggggatgaac cgcggcaaag gctggaaggc accgcagcga cagtttccgc ggcgattatg
    18001 aacggatgtc acatcgtccg ggtgcacgac gtggcggcca tgaaaaaagt ggctgcggtg
    18061 acggacgcga tgacacatcc ccggaaggtg atctgatggt ttacggaata ggggtcgatc
    18121 ttgtcgaaaa cagacgcatg gaaagaatca tcgatcgatg gggtgtcaag ttcttaaaca
    18181 gaatcttttc caccgaggaa atggcctact gcggcaaaca tgcttatccg gtgacgcact
    18241 acagcgcgcg gtttgccgcc aaagagtcat tcctgaaagc gctgggaata ggtttgggaa
    18301 tgggcataaa actaagcgac atttccgtcg tgaatcatga aggcggccag ccgtttctga
    18361 atctctccgg cgatgctcaa tcctacattc aaaaaagaag gatccacaag attcatttga
    18421 gtctgacgca tacaaaaaaa tattccgcag ccatggtttt actggaaaca atcgattaaa
    18481 agccggtgaa atgacaagat gagtcaggat gataaaacgg ttgagataat atccgtaagt
    18541 actgaaaata caatggaaat agggcgctct atcggagaag ggctccagga tggagacgtg
    18601 gtcgcgcttt cgggggagct gggttcggga aaaacctgtt ttaccggggg gctggcccgc
    18661 ggtttgggcg tggatgaaaa atacaggatc accagcccga cgttcacatt gattaatgaa
    18721 tatccggcgc gtcataggct ttatcatttc gacgtttacc ggatcagcag ttattctgaa
    18781 ctcgacgatc tgggatgcga agaatatctt ggaacagaag gcgttgttgt tatagaatgg
    18841 gccgagaaga tcaaaaagat tctgccggaa aaaacggttt acatcgattt tgaatacgtt
    18901 gatgaaaacc ggagaaaaat gatcatcagg gcgtccgcag acaggttaaa cgagttgatc
    18961 aaagatataa aaacggaggg tgtttaaata tggcattaat tgttcagaag ttcggcggaa
    19021 catcagtcgc gaatattgag aaaataaaaa acgtggctga aaaagtgatc aaggtcaaac
    19081 aggccggcca tcaggttgtc gttgttttgt ccgccatggc gggggagacc gacaggctga
    19141 ttaacctggc tcacagtgcg acggatttgc ccgatgaaag agaatatgac tccctgattt
    19201 ccaccggcga acaggtgacc gtaacccttc tggccatcgt tttgaatgcg atgggctgca
    19261 aggccagatc gtttctgggc tttcaggtga aaatattaac cgatcaggct tataagaaag
    19321 ccagaatttc gctgatcgat acggaaaaca tcaaaaagga acttaaaaaa ggaaccgcca
    19381 tcgtgattgc ggggtttcag ggggtggata aagacaataa cattaccacg ctgggacgcg
    19441 gcgggtccga taccagcgcc gtggcgctgg cagcggcatt gagagcggat cagtgcgaca
    19501 tttatacgga tgttgacggt gtttatacca ccgacccgaa cgtttgcaac aaagccagac
    19561 ggctggaaaa gatttcctac gatgaaatgc tggagatggc gatgatgggt gccaaagtcc
    19621 tgcagccgcg gtcggtggaa atggctaaaa aatacaatgt gccggtttat gtcaaatcaa
    19681 cattttcgga taaaggaggg acgcttgtga ccagggagga taaagatatg gaaagagaaa
    19741 tcatttcggg tgtgacgtat gatcgggatc aggtcaagat tacggttgtg catgtgccgg
    19801 atcagccggg agtggcggcg tctttgttca gcccgttgtc ggataaaaat atttttgtgg
    19861 atatgattat tcagaatgcc agttcggaag gctatacgga tctgactttc accgtatcca
    19921 aaaaggattt gaaggaagcg cagaaaattg ttgaaagcac ggcgaaaaaa gtcggagcga
    19981 agaaaattga agtcgatgat caggttgcca aaatatccat tatcggcgtc ggcatggcca
    20041 gtcattccgg cgtggccgcg aagatgttca aaacgctggc ggctgaaggc gtgaatatta
    20101 tgatgatcag cacatcggaa atcaagatat cctgcatcat tcaacgcaaa tacatggaac
    20161 tggcggtgtc ggtgctgcac gatgctttcg gactggataa gaaaaaataa cattgtattt
    20221 atttcagaga gcagactgaa ggggctgatc gttttgagcc ttatggtggc ggtcatcccc
    20281 tttgtttctc tgatgatcac ctcttggact ccttaccgga ttcccgttta tgccgacacg
    20341 tgcgatcact gctgtgtgat cgagatcgtc ggcgatgatc aaagggccgg aatttaccac
    20401 gttccatcgg ggatgtcggt caatcgcctg ctgaaaaccg cgggcgtcga aaagcaattc
    20461 gaaaaggact ttaccgtcaa aaccgggatg aagctggtga ttcgttccgc atcggaccgt
    20521 ccgggtatta cggtggcgga aatacaaaac acagcgaaaa tctctgtcgg attacccatc
    20581 gatgttaata aagccgggga agaagatttg attcttatca agggcatcgg accggtgacg
    20641 gcgaaaagaa ttctggaatt gcggacgcgg ctcaacggaa tcagcgacat ccggcagttg
    20701 atggagatca agggcatcaa ggaaaagaga atgaaacata ttgagaaata tctgtacgtg
    20761 gaaaagaaaa acgtctgaat cagttgccgc tgaacgatct gtaaaaactc aggacgcgtc
    20821 tgacataatt ttgtgtttcc cggtaaggcg gaatgttgta gttgtatttg gccacggcgc
    20881 cttcgccggc attataggcg gcgagagcca gcgacagatt gcctttgtat aaattgagaa
    20941 ggtatcgaag gtaccgcgcg cctccttcga tgtttttttc aggatgaaaa ctgtcatcca
    21001 cattcagttg cgatgcggtt ttcggcatga gctgcatcag gccctgagcg cctacggggg
    21061 acacggcccg gtgattgaaa ttcgattccg ccttgatgat tgctttgatt aaagcggagt
    21121 ccagtttgaa tttatcggcg gttttattga tgatgtggtc gtatttatta acgtcgagct
    21181 ggggctgaaa cagaatgcgt ttttccctca tgaccataac gtattttgcg ccggtatcgg
    21241 aaggaacatt ggtcagatgc aacacgcctt cattatcttc gtatttgtag atatcggcac
    21301 gggctatgtc cgtgaacgcg gcagcgaaaa aaaataacag tgccgcgatg acgattttgg
    21361 aaaataatgt tatatgaagt ttgaaaagcc ggtaaatatt attttgcatt tgattataat
    21421 actttatcat attggaaagt tcaatatttt tttgatcacg cacacaccgg tgcttgatcc
    21481 ctccgtataa accggatgct ttcgggtaaa cagcgtgaaa aaatcgagat ggcctcaaat
    21541 ggtcatgccg ccgtctatgg cgagcacctg ccctgtgatg tagtcggatg catcggatgc
    21601 cagaaagatg acggccccga ccatatcgtc cggttctgcc ggacgcccca gcggtgttct
    21661 gctaatggcg atattcagga tatcctgatt ggtccataac ggttcgctga actttgtttt
    21721 ggtcagactg ggtgcgatgg cattggcccg gatgttgtag atcgcccatt gttgcgccat
    21781 gactttcgtg gccataatga cgcccgcctt gctgatggaa taaaccggca ggatatcggg
    21841 agaaagaccg gcaaccgatg ctacgttgat aatacgtccg cctccttttt ccttcatgat
    21901 acgggccacc gcctggctca agaaaaacaa ccccttgagg ttcagattca tgatggaatc
    21961 ccaggcgcgg tcgtcaatat ccatcgccgg caccatggtc ggattggttg cggcattgtt
    22021 gaccagaata tcgattctgc cgaattcggc agcggctttg tcgacaagat tttttatttc
    22081 ttccagacgg ccgatatgcg ttgctaccgg aagacacttt cttcccagcg attttatttc
    22141 tccggccacg ttttccagat cgccgatttt gcggctggcg atcaccacat ccgctccgga
    22201 tttggccagt cccaacgcga ttgctctgcc gatgcctctg ctgccgccgg tgaccagcgc
    22261 cactttattt tccaacgata attgcatggt gttgcctcct gtgaaaataa tgattattgt
    22321 gccggcatat ttttgcggct gttcatttga cgaaagacgt ccctttcctt gtcatacatg
    22381 atttttgcct ctttccggga cgtattttca tgattgtaac ccagcagatg caaaatgccg
    22441 tggattaaaa gaaaatcaat ttcttcttcc aacatcaact ttgcccggat ggcgtcccgc
    22501 cgggcggttt ccacggaaat caccacatcg cccaggatct ggggattgat atgtccatac
    22561 tcgccttcgc gcatggaaaa ggaaatgaca ttggtcgcct tatcccttcc cagatagcac
    22621 ctgtttattt cccggatttg ttcatcatcc gtaaaaagga tgctgatgtg ccggtccgta
    22681 cattcgagga ttttgaagat tttggaaacg gcgctgcgga tttttctgcg ttcaatcttg
    22741 tgcagggttt gcctgttttc tatttgtatt ttcattggac tctttcttcc cgttgccttc
    22801 cttctggggc gcttccggat aatcgatacg atgatggaag atgccggtca ggattttggt
    22861 aaacgtctcc gcaatggtat ttaaattgct gagcgtcaat tcacattcat ccagttgacc
    22921 gtccatgtaa attccttcga ttctttcccg cacgagcgac ttgattctgg ccggggtcgg
    22981 attggtcagg gtgcgtgaag aggcttcaat gacgtcggcc agcatgatca gaccggcttc
    23041 tttggtttgc ggtttggggc cggggtagcg aaaatcgttt tccgataggg agcgaaggga
    23101 ttcattcttg tccttcttgg ctttgtcata gaaaaagctg ataatgctgg ttccatgatg
    23161 ctgacggatg atgttgataa tttgacggcc cagtttggct ttggcggcca gttcgcagcc
    23221 gtctttgacg tgagacatga tgatcaggct gctcattttc ggcgataatt tgtcgtgccg
    23281 gttatccgag ttcggctggt tttcaatgaa atactgcggt tttttcagct tgccgatatc
    23341 atggtagtac gcgctgacct tggtaagcag agcattggcg ctgattgctt cagccgccga
    23401 ttccgcgaga gacgaaacga taatgctgtg atgatatgtg cccggcgctt caataatcat
    23461 ttgctgaaaa agcggctggt tgagattggc caactccaac agtttgatat ccgtaataaa
    23521 accgaacagg ctttcaaaca gcggcgtgat ccccgccaca agaatgccgg tcacgatacc
    23581 gccgatgaat cccatgatca atctgatcgg cagatcattg aaaaaatttc ccgtgatgag
    23641 attaaggcag acaatcgccg ccatattgat aatgcccaga aagagaccga tcttcaggaa
    23701 gacggaacgc tgacggatgc gcacgatctg ataggatgcg gcaatcgatc ccagaaaaga
    23761 aaacagcggg aaaacgattt tttcatcgaa gaggaatccg atgagaaaag acgtcagcgc
    23821 gctgataatc agcgcgacat tccggttgat cagaatagcg atcgtcatgg cgcccacggc
    23881 aaaggggatg gcgtaataac aggcatccac cggtataaaa gagaacgacc ggttgatcgc
    23941 gatggacata aaaattccga ttttgataaa aaggatttgc agcagggcga tgatgccgaa
    24001 aaccagcaaa tccaggttgg accggtcgga cgttttgacc cagtttcttg tgcgccacag
    24061 ataaagcacg atggaaagaa acatggccgt gaagaaaaat cccagcacga tcgcgactcg
    24121 ggtaaatcgg ttgtcctgat cgtctttaaa gtaggcgttt agtttggcca gttccagata
    24181 gccgattttt tctccctccc ggacgatcat ctcatttttt tgtacctgga aaaacgtggc
    24241 ggctgcatca ccgatgatcg ccattttctt tttctcagtg gcgcccttgt taaacgccag
    24301 gttcggttca ataatttttt tcgtgatgga gaatgcggca tctttcaagg gtttctcatc
    24361 attccggaaa atcatgttga tggttttaag cagagggtcg tcgatttcat tcatgttcag
    24421 cagggcagac gtgtccctga tgtcttcgga aatctgggtt ctcaaattcc ggataaccac
    24481 gcctttctgc ttttccgatt tgccgaaagc atcgctggcg ataaaccggt tgtcataaaa
    24541 ggaaatgatc agttgggaaa atttattctg caactcctcc gaaaatctgt attcgttcaa
    24601 aatatagaat tcttccggtg tcaaataggc gcccagattg ttctccagac gttttttctc
    24661 tttttgcaga tccatgattt tgtcgggctg ctgctgtttt tcagggacat tgttgtatcg
    24721 ctccgcggca agagaaaaag ccttgacgat ctttttttgg atattatcgg caatctgact
    24781 gttgtaattg taaataggat tgatattttc cgcgtcctgg atttttttct cctcggcggc
    24841 ctgcgggtcc tccaccagaa aagcgaagtc cgccttaatg ttctttggag caatcatgcc
    24901 gatacggaat tgcggtttgg agaaattcag ttccggcgcc atgatgaggg ccagaatcag
    24961 acacagcacc acggcgatca tccagcgctg gaaaaggctg tttctcacga aaccgaaata
    25021 aggtgctgct ttttccagat agttcctgaa aaattccgtc atttttattc tgagcgattc
    25081 aaaatatttc atgatcacac gttatgatga ttggtatttt ttgatttagt cagatgggca
    25141 taggccttga taatgtcctg aaccagcggg tgccggatga catcgatttc cgaaaaatgc
    25201 acaaaacgga tgccggcgct ttttttcaaa atgttttccg cttcgatcag accggatact
    25261 ttttccgttg gcaggtccac ctgcgtgata tcgccggtaa tgacggcttt ggacccgaag
    25321 cccaatcggg tcaaaaacat tttcatctgt tccgacgtgg tgttttgcgc ttcatcgagt
    25381 ataataaacg agtcattcag ggttcggccg cgcatgaaag ccagcggcgc cacttcaatc
    25441 agacctttgt gaatcagagc ggtggccctt tccatgtcca gcatgtcgta gagcgcatca
    25501 tagagagggc gaagataagg gttgaccttt tccgccaggt ctccgggtaa aaatccgagc
    25561 ctttcaccgg cttccacggc cggacgcgcg agaataatgc gttggacctt tctttccata
    25621 aggcaggaca ccgccatggc catggccaga taggtttttc cggtgcctgc cgggccgatg
    25681 gagaagacca tgtcggcatt tctcatatat tcaatgtaaa gccgctgggc gatgcttttg
    25741 ggagtgatca cgcgtttctg cgaggaaata aaaacggtgt tcaggaaaat actggccagc
    25801 tcgaaggtaa cgtcctcggt cagaatacga tgcgcataat cgatatcggc ggattgaatg
    25861 tgccagcctt tttccatgag ggcatacatc tgctgcagca tcgttttgat tttatggacg
    25921 ttgccgtttc ggccggagat ggtgacactg ttgccccatg ctttgacttt gaccggttcc
    25981 agcttttcaa tgaattttaa atttttatca tgttcgccgc agagattttt cagcagatgc
    26041 tgatcggcaa agacaatttt ctccgcgtct tcatttttta atgcttttat caaaatagtt
    26101 atcaacctct acaataggtg atcaatattt cattcaacgt tggtatctat cattttgatt
    26161 tcggcattgc aatacctatt gataaatccg taaatgcttg aattataagt gctttatgca
    26221 tcatggtatg atgatgtggt ctgttttcat aaggatgccc tcatgatttc cgcaaaaaca
    26281 gataggcgaa gaccctggca ttcccgatca tattttcgac ggggcagttt tcgtcgggtt
    26341 gatgagcaaa ctggttggtt ttggaccaga cggcaaccgg atagtttttc ttgcggacat
    26401 aggaagcgac ggttccggct cccacaccgc cggtaaaggc tttcacatga tagacggcgt
    26461 caatagcgtc ttgcagcgcc aggaccaccg gagcatccga cggcgtcggt tttgcggact
    26521 gaacatactg aaccatggac gtttcgatcc gcacattgaa ttttttctca attttccggc
    26581 agatcttttg tatggcattc attacatcct taagatcgta ttccggcaat acccggcaat
    26641 ccatacagaa cacatcttcc ccgggaatcg tgttgatatt gccgacattg gcttctttct
    26701 ttgtcggttc aaacgtgctt tgcggcggat caaaaagagg atcggtgtcg ggaaacattt
    26761 ttcgcagttt gatcaggctg gtgaccagat gcgatccggc aacaaaggcg ttgttgccca
    26821 ggtggggggt cgatccgtgg cattgtctgc ctttggtttt gaaacagatc cagagcatgc
    26881 ttttttccgc aacttcgatg agcgatcctt cggcgttgcc ggagtcgggc acgacaatcc
    26941 aatcgttatc ctgaaaaagt ctgtccgtag aatcaatcat ataaaacaat cccttactgc
    27001 tggaggtttc ttcgtcggac acgaacgcca gtttgatcga gttttcggga accacgcctt
    27061 catccatcag cgctttggcg gcaaagatgg aggcgaccag atcctgctga ttgtcttcca
    27121 cgccgcggcc gtaaatccgg ccgtctttca cgtaggcttt gtacggatcg ctgctccaga
    27181 ggcggagttc gcccggcggg acaatatcca tatgggtaat gatccagaca aacctgtttt
    27241 tgtttttccc ctcgatagtc gtgacaaaat tggggcggat tccggaagac actctgttgt
    27301 ccggagcgtc gtagtgctgg aagtttttga atcccatctg cagcagacga tctttcagaa
    27361 aattggcttt cacaagttct ccgtcgccgc cgttttccgg tccgacggcg ggatgcgcgc
    27421 aaagcgccgt ctgcaattca atcatcccgt cacgataagc atcgatccgt tgcgatattt
    27481 tttgaaataa tttttcgtcg atcataattc atcctttgat ggtgatttag tctatagggg
    27541 cgataacggt gtagccgccc ggcggaaggg gatggtccgg atttttgccg tctcccagaa
    27601 tcagcaccgt attctttgtc tgatccaggt atttaacggc ggcatgatgg aggtcctgca
    27661 cggtgacccg gttgatttta tcccggtagc tcgtcagaaa atccggaggc agtttttcat
    27721 actcgatggt catttgctgc cggacgattt gttccggggt ggcaaatgaa aagaggaacc
    27781 cgtcattgat tgatttttgc gcccaggtca gttcctgacc ggcaaccgga acggctttaa
    27841 tgttttccag aacggtattg atcagggaaa gagccttcat ggtcgacgat gtttttgtaa
    27901 aggcgtagct gccgaaaatt ccgtaatcag gcctggcgcg gtaaaaactt ccggcactgt
    27961 aggccagccc ttcattattg cgcacggtgc tcatgatccg ggaaggaaat ccgccgctgc
    28021 cgacgatgaa atccaggacc gtgaaggcat gaaaatcggg gtggtttttt ccggtggcga
    28081 attggccgct gataatcgtc gactggggaa tatccttttc gataaaatac ataccggttt
    28141 gcggttgttg caccggcgcg ggaaaatccg ttgcactgtt tttcgtattc cagccgccga
    28201 aatactgttt gattttctca acggcttttt cctttgtgat gtcgccgctg acggcaaaca
    28261 tgatgttgtt cggcaggaag aaacggctgt gaaacgccgt cagatcatcg cgtttgattt
    28321 ccgtaagcga tgcgctggac ggaaaacgtc cgcgggggtc ttgatgatat atcagacggg
    28381 agaattcgcg catggccagc ttttgcgggt catccttgat gcgcctgatt tcctcaagtt
    28441 gtaattgccg ggccagttcc agtttcccct gttcgaaaac gggatgaatc agaatttgcg
    28501 acaacagatc cagccccttg tcaagatgcg tggtgagaac ggaaaaaccg acatgcgccg
    28561 attccatggc catggcaatg gaagccgatg ccgcaatgag atcaaattgt ctgtcgattt
    28621 cccggctgct taatcgggtt gtaccgccgg ttctcatgac ctgagcggtc agctcggccg
    28681 tgccttcttt accggcagga tcatgcatcg agccggtttt cacaaaggca ttgatggtca
    28741 ccagaggcag gtcgtgatct tcgagaatgt aaaggatgat gccgttttcc aaaacgaccc
    28801 ggtgcgcctt cggcaattca aaaaacaagg gttcgtattg aagctggtcg ggagaaggcc
    28861 ttgccgcgtc ggcctggtta gcgctactgg gcctgacggt cgccatcatt aaaaacagaa
    28921 acacgccaac cagaaaaacc ggatatattt ttgaaatcat tttatccatc agacgctacc
    28981 cgccttcttt attgagaaaa gctgttgtgc ggttattgct gttcaaatac cttgccgcga
    29041 tttgtcgaat gtctgcggca gtgactttgt caatattgtc gagataatct gcaaaatatc
    29101 tgtaatcccc cagcagaacc tcataatagg aaagaatgga agcaatatcg gcattggcat
    29161 cgaggctctg aaggtaggac attttgatgt gattttttgc cttcatcagt tcctcgtcgg
    29221 aaacgggttg agtcttcagc ttttcaattt ccttcagaat atcattttcc agctcgatgt
    29281 tgctgtgcgg atgacgcggt tgagcgaaca gggcaaatag attcgggaat cttgaaccgg
    29341 gcaaaccgtt gacggcactc acgctctggg caatctgccg atcgatgaca agggtcgtgt
    29401 acaggcggct ggttcgtcct ctggttaaaa ttgtttctat gacatcgaag acgtaatcct
    29461 cccgcgccgg agcattcggt ttgcgaaagc cgatgatcag cgacggattc gcgtcgaagg
    29521 caacattgac ccggcgttct tcggtctgag gcggttctgc gggaatataa ctttccgttt
    29581 tttcagccgc gggcggaatg ccgccgaaat atttctcgat taatttcagt gtcggttccg
    29641 gtttgatatc gccgacaacc gcaatcacaa tgtttcgggg atccttatat ttttccagaa
    29701 ttttttccag ttcatcgggg cttaaattca tcaagtcttc tgtccagccc agaaccggcc
    29761 tcccgtaagg atgaagggtg tacgcggcgc tcatgaattt ttcataaagt tttccctccg
    29821 gatcggattc cacccgctgc cgtcgttctt ccatgacaac gtcgcgttcc gtataaaatt
    29881 cacggaagac cgaatggagg agacggtccg attcaattct tgcccagagt tcgattttat
    29941 tggcgggaag actgacgtga taggaggtga catcctgacc ggtggacgca ttcatatcga
    30001 gcccgccgtt ttcggtatag agccggtcga tttcgttggg taaaaagtat tttttatgtt
    30061 cgtcctgcaa cttttttaat acagcggaaa ggttggccag ggtttccgga tcggccgttt
    30121 gtcctttccg tttttcggca tccagacgcc gtccggcatt tttgatttct tccatcaggg
    30181 ccttctcggc ctgataatcg ttcgtgccga tggaggtggt tcccttgaac attatatgtt
    30241 ccaggaaatg cgccgcaccg ctctcgcctt ttgtttcatc caccgcgccc acccggtagc
    30301 gaatgtagag ggatacggtg ggacttaaat gccgcttaag catgagcacg gtaatgccgt
    30361 tgggcagctt ttgtttcaga accttttctt tcagatcgga ggcgtgaccg gagcctgcgg
    30421 caataaaaat aaaaatgaaa gccggcagaa gagccgcttt cagtatttta cacgctttca
    30481 tctttgttca acccttcctg cttctgcgga aataaacgaa agataaaata aaggattccc
    30541 acaatacaaa ccagaatcag aagcgatgcg gaaaagaaat acatcaaaaa ttccatcgga
    30601 ttattgatcc ggtacgaccc catcagcgta ttgatgccga tgagcagaaa aaacagcgac
    30661 accagcagaa tcagcacatt tttaatccat atcaaaacct tcatgaaggg gcgcctgtcc
    30721 ttgaaccgtt tttgagtttc tttttcaatt ccgtataacg cgcaaaactg aaatcatcat
    30781 ttccgcaggg aagcaatgaa ttgttatgat acaggggttc gtgaagaatg tcaaagggtg
    30841 aacaggccac ggcctcttgc cacatggcgg ttccgggaat cggggagtat tcggccagca
    30901 ggggcttcgc gccgcattcc tgtacaaatt caatactgtt gatgacctcc cggacggtct
    30961 gaccgggcgt tccgcacagc aggtaaatcc ccaggtcctc tgttttataa ccggcgtttt
    31021 tcagatgttc aaccgcctga cgcagttctt catttttgac ttttccgccg gtttgccgct
    31081 gccggtccaa atcggatgtt tcaaaaccga aacggatggt cttgaatccg gcatggtaca
    31141 gccgttcggc aagttctttt gtgatctccc ttaagtgcaa tccgttggga caatggaacc
    31201 ggcaggataa atttcttctt tgtaattcat cgagcagggg cacgatcatt gcctccgggt
    31261 taaccagcag agcgtcatcg taaaaactga aattcaccac gccggttttc ctgtgccagt
    31321 attcgatttc atcggcgacc ttcaccggat cgcggcgcct aaatcccggc gtcagaatgt
    31381 gagacgaaca gtaactgcag cggtaagggc atccccggga cgtgatgacc ggcagttgcc
    31441 cggttttgga gatcagatca aaggcggggt aggggcagga atccagattg ttttcatccg
    31501 ggacaaacaa cgttttttcg ctaaatacct tttccagcaa atcgagaatt tgtttttcgc
    31561 ccgcccccgc gatgacgtaa tccgcccccg aaaagttgga cgcgtgatcg taacagagcg
    31621 tcgcgtattt gccgcccagg acaaccggaa tctgcggcaa ggtgtctttg atgattctga
    31681 tcgcttcaaa aacgccgggg taccagtagg tcatcatgga cgtcattaga atcatgtcgg
    31741 catcccggtg cattttcagc tcggcggcga aaacgtccgg cagaataccg taacggcagt
    31801 aactgcgatc aacccatttc agcggcgccg gtttgtcaat gatctgccgg taaaatttac
    31861 cgtctccgga agcataacgc cgcggtttct tcagccccga tgccgccatc tgcggatgaa
    31921 acggatcgag gcagtcgatc aaatcaatcc ggtggttgtt tttacgcaaa agactgccca
    31981 ggtatagcag gccgagaggt ttgatccaga agtcataggc cgcaaaatcg tgaatccagg
    32041 gattaatcaa aaggattttt ctgctcatcg gctggttctt atcacaatgc aggttcattt
    32101 acaaaagcct aaacgtttat gattcggccc ggcgcagact gccgcaggag gggcaaaatg
    32161 aaggggcgtg atcaattgac ttcattcatc ttttatgcta atcagttcca ccacgcataa
    32221 aagttattga aaatatgtag ttatttattt tgaggcctga aaagcgtgaa aaaaacgaca
    32281 ttgacaagta ttttttttag gtcttttttt attcatgcga cgcttaattt ccggcggatg
    32341 cagaacatgg ggttcggcat ggcgctcatc ccgatgatcc gggagttgcg cctgcagaaa
    32401 aaggatgcgg taaaaatctt aaccgctcat ttgcagatgt tcaatacgca tccgtacctg
    32461 tcggcgccga tcatcggttc gatcgtgcgt cttgaggaag accatgttgc gaaaggagaa
    32521 aattcggaca ccgcgtccat cagacagagc ctgatggcct cttatgcggc aatcggcgat
    32581 atcttttttt ggggagcctt cagaccgttt gccgcgatcg ttgcggtgct gctcgtgcat
    32641 atcgggttgg ccatggcgcc ggttatcttt cttctgattt acacgccggc gcatttatgg
    32701 gtcagattaa aaggatttat cgaagggtac cgcaagggga aaaccggttt tgaatttatc
    32761 cgcgccctga atttacccgc tgccgccgtc agaatacgct ggataacgct gatggttctg
    32821 accggtttaa tgatctggct tgcccgagac ggcgggtact ggcccttcgg ggaaacatac
    32881 ggggtggtcg caaaactgat tgccctggct acggtaatgc tttgttatat tttaataaaa
    32941 aaaggaattt cacaggttta tatcgtttat ggcgctgtgt tggcattcgt gattatttcc
    33001 tggacgggat ggattagttg acggaaacga aaaccttacg attaaaaaac aagctgggga
    33061 tgcacgcgcg ggcggcggcg tctttcgtga aagtggctca gcagtttcaa tcggaaattt
    33121 atattgagca caacgggcaa accgttgacg ggaccagcat cctggatatc ctgaccgtgg
    33181 cctgcccgta cggcggagag ctgacgatca aagccgacgg cgcggacgca gccgcggcga
    33241 ttgaggaact ggtcaaactg atcgaaaata aatttggaga agaataaaca tcatatgagt
    33301 ccggatcaga ccacaaaaac ttttgtcctc aagggagtgg gtgtttcccc cgggatcgct
    33361 attggaaaat gctaccgcta tgatccgctg gacagtcaaa tttcttttta taaactcaaa
    33421 gacgaatctt taatcccgga agagataagc cgttttaaaa acgccttgaa ggaatccgag
    33481 cagcaactgc tgaagattca gaaaaatctt aagaaagcca atgttgcgga accggtttat
    33541 gtcatcgacg ttcacatcct gattttgaaa gacaaaatct ttatcaaccg caccatcaaa
    33601 tatattcgcg agatgagcgt caatgccgaa tgggcgctgc gcatgaccct ggatcattat
    33661 aaagaggtgt tcgaaggcgt tgaagacgat tacatcagca gccgggtcag cgatgtgcag
    33721 tatgttgtcc agcggatttt gcgcaatctg tccggcggaa aaagtgaaat cgtctgggaa
    33781 acggaaaacc ggggcgtcat tatcgtatcg cacgacatct caccggccga tacggcgcag
    33841 atgaaactgg acaaaattct gggatttgcc acggacagcg gcgggcgcac ctcgcatacg
    33901 gccattgtcg cgcgctctct ggagctgccg gccgttgtgg ggctggatga tatcacgcgc
    33961 tttgttcatg aggatgatga cttgatcgtc gacggcacgg ccggcatggt ggtgatcaat
    34021 ccctatcccg acatgctcaa gcggtatgag gaaaagaaac gccattacga cgccgcaaac
    34081 gatgaatatc tcaaatacgg caggcagccc gcggtcagcc tggatcagca ccgtattcaa
    34141 atcggatgta acatagaatt tatagaagaa attccttccg ccgtcgctca cggcgctgaa
    34201 aatatcggcc tgtaccgcac ggaatttctc tacatttacc gcgaaaactt gccgaccgaa
    34261 gaggatcatt tcacgaatta ccgccaggtc gtgaccaata aaaatctggc ctggtcgacg
    34321 atccggacgt ttgatctggg cggcgacaaa tttcccaatt atcagaaaca gagtaaggaa
    34381 atgaatcccc agatgggcat gagggcgatc cgcttctgcc tgaaggaagt cgacattttc
    34441 aagattcagc tccgggccat ctggcgcgcc agtgcgctgg gcaacattaa aattctcttc
    34501 ccgatgattt ccagtgtgga cgaaatccgg gaagccaaaa aaattctgga agaagcccgc
    34561 cgggaactga tcgatgaagg aatgaagatt cctgaaaaaa ttgacatcgg tgcgatgatt
    34621 gaagtgccgt cagcggttgt gattgccgat aaaatcgcgc gggaggttga tttcttcagt
    34681 atcgggacca atgatctgat ccagtattcg ctggcgattg accgcgccaa cgaacgcctc
    34741 acctatctgt acgagcccct gcatccggct gtcttgagac agattaaaaa agtcgtggat
    34801 gcggcgcaca aggcgaagat cgaggtgtcc atgtgcggcg aaatggcggg tgatccgctt
    34861 tgtgtgctgg tccttctcgg tatggaactc gatgaattga gcatgaatca tctggccatt
    34921 ccgagaatca agaaaattat cagagaatcg acattgaaag agtcgaaaag catgttgaaa
    34981 cgagcgcttt ccttcaatac ggcttcggaa gtccgctctt atgtgcagaa atatatggcg
    35041 aggcgtttcc ccgaagattt caagacggaa gattaatgta cgggaccgcc cgtcaaagtt
    35101 gtgtggagaa ataacgacga tggaagaaaa taatcatccg gtaaaatcca atcagggcat
    35161 tcaatactat tcgggtctgc tttacgacaa tcagaatttt ctgcaattcc ggctggccaa
    35221 gacgtttctg tcgcgtgttt ccattgccga cgaatacgtt gaaaatatca aaaaactttc
    35281 cggcgagggc gtggtcgttt atgccttaaa ccagcgcagc gaactcaaca gccttattct
    35341 gtatgagctg ctccagcgca acgacatgcc cgttccggtc tattgccacg gcatgaacat
    35401 gtcgttggct catccgttgc cgcaaatgct gaaatttttc tggtcgtctt taaaacgccg
    35461 gatgggcagg gaacgaaaag cctggcaggg caaactggcc ttcattgaaa aaacaatcat
    35521 ggagaaaaag agcatcgtga ttcatctggg cgaatcggag ttcattgaaa accggtcggc
    35581 ggaaagcgcg atgtccacct tgcttgatat tcagaaaaaa gccggttttc cggtttttgt
    35641 cgtgccgatg ctggtatcgt acgggcgccg ccgtgaaaaa gaagacgagg ggctgatcaa
    35701 tatccttttc gggcagaccg agcataccgg ggcgctgcgc cgtctgatca cgtttatccg
    35761 atattcccga agcgcatttg tcattccctc ggagccggtg aatttgtcgg agtatctgaa
    35821 aacgaccaag aaaaccgcca cggacgccat ggttcatgaa ctgcgcggag aactgattga
    35881 tcgcatcgac aaggaaaaga cggccattgt cggtcccgcc ctgaaatcgc gggaagaact
    35941 gatcggcatg gttttaagcg atgaatcgct gaaagccttt atcgaaaagc atgcgcagga
    36001 ggagaaaaaa gacaaggcca ggattaaaaa agatgcacgc cgttatctgt atgaaatagc
    36061 ggccgactac agcgaaacct tcatcgccat ctggaaaaaa gtgcttactt ggctgtggaa
    36121 cgatatttac gacggcctgt ccattgatgt ggaaggcatg gccaaaataa gaaatatttc
    36181 caaaaaaatg ccgtttgtga ttgtgccctg ccatcgcagt cacattgatt atcttctgat
    36241 tcattacgtc ttctatatca ataacattca gcttcctttt atcgcagccg gaaacaacct
    36301 gtcgtttttc ccgatggggt acattttccg gaaatccggc gcattctttt tgagacgaag
    36361 ctttcgcggc aacaaggttt attccgaagc ctttaccaaa tatctggcga tccttctcaa
    36421 ggaaggactg ccgctggaat ttttcattga aggcgggcgc agccggaccg gcaaaatggt
    36481 gatgcccaaa tacggccttc tttccatggt gcttcaggcc tatcaggaga aatactgcga
    36541 aaactttgcc gcgattccgg tgtatatcgg ttacgaccgg gtgatcgaag aaaaatcata
    36601 cctgaaggaa ctgtccggcg aaaccaagac gccggaaaac acggccgaga tcattaaaac
    36661 cagcaaaata ctgaccaaac gattcggccg cgtgtatgtg aacatcggcg aaccgatgat
    36721 catgaaagac tatctggcgc agcaggcaaa accgctggaa gacatgtccc tggaggagag
    36781 gcaaagcctc taccggaaaa tcggttatga aatcgttctg gaaatcaaca atgtgtccgt
    36841 ggtcacgcct tttgctctgg tggccgccgt tattttaagt catgaccgcc gcggcatttc
    36901 ctacaacgat ctgctgtatg ttttgaatga attttacgaa tatctcaccg tcaaacaggt
    36961 gaaatttgcg gaaaccttcg cccatcgcga caaggccatc atcgatgcgc tgaacatgtt
    37021 tgtgcaggca ggcttcattg aaaaaattga agcggaaaaa gatgaagtcg aggaaatgca
    37081 ggaagtcgtc tactcgctga aggaggaacg aagactcaat ctggaatatt ataaaaacaa
    37141 tattctgcat ttctttgtcc cgctgtgttt tgtcgccacc tccatcatca aaaacaatga
    37201 agacctgatt tcgctgaatc gcatacaggg cgactacggg tttctgaaaa aattattatg
    37261 gaatgaattt atttttgaca aacacaagga cgaagcggag gaagtcaatg aagtgctgtc
    37321 ttatttatac gaccggaaaa tcattgtctc ggtcgaacag gacggacaga ccctgatcga
    37381 aatcaaaggc cggggcaaca aacgacttaa accgctcgcc gatctgattc acaattatct
    37441 ggaatccttc tggatcgtga tgagaagctg tctgtatctg aaaaaaaatc ctctgcccaa
    37501 aaaagaatgg ctgaagaaaa tcatggcgct gggcgaccgg atgtaccgca agggagaggt
    37561 gctgagaaaa gaagcgctat cgcaggccaa ttatcaaaac gttatcatct atctggaaga
    37621 cgcggatctc atcaaatcgt tccgggatga gaaaatcgat aaaaaagatg ttttatacac
    37681 gctggctgaa aacagagcgg aaatggaagt gctgcgccgc cgccttttca gatttttata
    37741 atcattgacc gccgccgatg cgcaagccgc atcgccgagg gggttgatcg gatcgggttc
    37801 agaccgggag agccggcctt attcaaaagg ccggctcact gcatgttgcg gggttatctt
    37861 tttctgccgg atttcgccgg ttgtttcatg ttatagaagg tctctttgcc gcgatacaca
    37921 gccgcatcac cgagttcttc ttcgatgcgg atcaactggt tgtatttggc cagccggtcc
    37981 gtgcgggaga gcgagccgct tttgatctgg ccgcagttgg tggccacggc gatatccgcc
    38041 ataaaggtgt cttccgtctc gccggagcgg tgagaaacca cgcacgtgta gccgcattcc
    38101 ttggcgcggc tcatggtttc cagcgtttcg gtcagcgtac cgatctgatt gagtttgatg
    38161 agaatagagt tggcgattcc cagatcaatg cctttgttga gacgtttgac gttggtaacg
    38221 aagaggtcgt ctccgacgat ctgaattgtg ccgcccagtt cttccgtcat cactttccac
    38281 ccggaccagt cgtcttccgc caggccgtcc tcaatggaaa tgatcgggta tttggcaacc
    38341 aactcggcat aaaatttgac cagctcttca gacgacttcc ggggcttggc ttccgccgcc
    38401 agaatgtatt tccctttttc atagaaagaa ctggctgcgc agtccagggc gatcatgata
    38461 tcccggcctg ctttataatt ggccttgtcg atagcggtca taatgcatgc gagagcttct
    38521 tcattggatt tcagattcgg agcaaagccg ccttcgtcgc cgacagaggt attatagcct
    38581 ttacctttca ggacagactt caacgcatga aagacttccg cgttcatgcg gatgccttcc
    38641 ttgaagttgg gcgcgccgac cggcaggatc atgaattcct ggatgtccac gttgttgtcg
    38701 gcatgttgtc cgccgttgag aatgttggac tgcggaacgg gcagaacatg ggagttgacg
    38761 ccgcccagat agcgatacaa cggaagcccg gatgcttccg cgccggcctt ggcgcaggcc
    38821 atggagacgg cgagaatggc gttggcgccg agtttcccct tgttggcggt gccgtccatt
    38881 tcgatcatgg taaaatcgat gtcgcgctgt ctgcggcaat ccattccaat gatttccgga
    38941 ccgattttgt gcaggatgtt atcgacggct ttttcaacgc ctttgccgtt gtatcgtttt
    39001 ttgttgccgt cgcgcaattc cagcatttca tgttctccgg tggacgcgcc ggacggcacc
    39061 gcggcccgcg cggtgattcc cgatagcgtc gttacctcgg tttcgacggt cggattgcct
    39121 ctggaatcca gtatctccct ggcatgaata tggatgatct ccggcataaa acacctccct
    39181 gatgaacttt cctggaatgg taacagagag gccttgcgat ttcaacagat tgatgaaaca
    39241 aacttgatta cttttcataa ctcatataga tagaacccgg tccgtgagaa aaatggagtc
    39301 aatggaattt aagatgaatc aggatacaaa agacaggaac accaaacttt tcctgcatat
    39361 gaaaaaatgg ctggaaaaag ccgtgctgga ttacaaaatg atccaaaagg gcgatcgtct
    39421 gttaatcggc gtgtccggcg gggcggacag tctggcgctg ctggacctgc tcgactctcc
    39481 gatgcttttc attcccgatt tttccttcgt ggccgtcaac attgacatgg gattcgaccc
    39541 cgcctataaa aaatacgaca gactggaaaa atattttcag aaaaataatt atccgtatgt
    39601 gatggaaaag accgatatcg gcactgtggc gcattccgat gtgaataaaa aaaatccctg
    39661 ctttttgtgt tcccggctaa gacgaaaaag aatattcgag atagccgatg ccgagggctg
    39721 caataaaatc gcctttgccc atcaccgcga cgatattatc gaaaccctgc tgatcaacat
    39781 gttttacggg cgtgaaatca gcaccatgat gcccaatcag agcatcttcg gcggtaagct
    39841 gcacatcatc cgcccgctgg tttatctgag cgaggccctg gttaaaaaat acagcaggga
    39901 acggcgattt cctgttttga aaaatgactg cccgacaagc aaaacatcaa agagggtgta
    39961 tatcaaaaac cttctcaata aactggaacg ggacaacaag gaaatccgcg ataacattta
    40021 caaggccatg agccacgtca aaccggacta tttgctgccg caaaaaaagt gaaaagagaa
    40081 tggtgaatag tgaatgttgt atggtagaca gtctgaagaa aaaactatag caatacatcg
    40141 tatttcatgt aaaattgcat tatggcaaaa gagaaaacag cacaaaaact gatcgcgtcc
    40201 aataaaatgg cgcgggtgaa ttatgaaatc ggcgatatct acgaagcggg tcttgtttta
    40261 accggcacgg aagtcaaatc gttacgagcc ggcaaagcca atctcaagga cagctatgcc
    40321 gttgtcaaag acgatgaagt ctacatcaag gaaatgcaca tcagccccta cgatttcggc
    40381 aaccgctcca atcacaatcc gctccggacc cgcaaactgc ttttgcacaa gcgcgaaatc
    40441 aaaaaactct acgggaaatc gcgcgagaag ggattgtcgc ttattccgct caagctttat
    40501 ttcaaaaacg gcaaggtcaa agtggaaatc ggcatcggca agggcaagaa actttatgac
    40561 cgccgtcagg atatcaagca gcgcgaagaa agacgctcac tatcccgcga tttcaaatcc
    40621 aggcagataa aagtctagtg attatgaatc catatgacac ggattaaggt gaaatttgac
    40681 aaaacacttc taagtgttta tattacatga aaatacgtaa taggggggcg tcacggtttc
    40741 gacggggaca tttgaagttg tagaagcgtg ccgaggttcc tacaggc
//
LOCUS       DAONQZ010000017        37016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1236_length_37016_cov_13.937258, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000017 DAONQZ010000000
VERSION     DAONQZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 37016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37016
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_37016_cov_13.937258"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..453
                     /locus_tag="PLR13_04730"
     CDS             190..453
                     /locus_tag="PLR13_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18671.1"
                     /translation="MPPAEMRHERVVVETDRYRVEGDITLPQEGYRSRLSDYLNRSDQ
                     EFIILVNVELLSLDGSGRDWRAPVLMLARQHIRLVVPVGGPNA"
     gene            663..1448
                     /locus_tag="PLR13_04735"
     CDS             663..1448
                     /locus_tag="PLR13_04735"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQC18672.1"
                     /translation="MNVKDLFDLKGKISVVTGGGRGIGKFIATGLAEAGSDVVIASRK
                     MENLEKEVKELENLGIRALAVKCDLAKKEDIGNLVKAAMDKFGTIDILVNNAGLTWGA
                     PTLDYPLDKWEKIFDVNVKGVWILSQQVARIMKEKGGGKMINVSSIYGSRGSNEEEHP
                     AVAYNPSKFAVEGLTKTLALKLARYKIYVNCIAPGFFKTDMMGHLFKPEMKPILDLTV
                     GAIPLKMYGEEDDVKGLAVFLASKASNYITGGIFPVDGGFSAQ"
     gene            1525..2625
                     /locus_tag="PLR13_04740"
     CDS             1525..2625
                     /locus_tag="PLR13_04740"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQC18673.1"
                     /translation="MKKIKHSFFLLLSLIVVACAPAYTLNHSSPVKPLSLPADAAAHY
                     WAQNEWWYYTGHLKAEDGREFGYELTFFKRITSEDKVPHFFIPIPAHWLKDLGMMGHF
                     VITDITHQKFMTQEVNNFFGLPSKADDTKYDLMIGTWTAREEEGKHLLYAEMDDGRIE
                     LELQPVKVAALHGRQGIVDKGGGNSNYYYSLTNMKTSGTLTIGGESLDVHGKSWMDHE
                     YGTMKLTYPQSGWDWFSIQLENHYELMIYVIRSEGDLKNSLVGGTFVLPNGKTIPVKA
                     RDIEIRNLDYWHSEKTDSDYPSSWEIIVRPFKLKLKCQPVMAEQEVNLQPMPYWEGAI
                     SVSGTFHGKSVKGQGYIELVGYSRKYPIQYIN"
     gene            2763..3512
                     /locus_tag="PLR13_04745"
     CDS             2763..3512
                     /locus_tag="PLR13_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQC18674.1"
                     /translation="MSGHSKWSTIKRKKGAIDAKRGKIFTKIIKEITLAARLGGGDIE
                     GNSRLRQAVMLAKEENMPKDNIERAIKKGTGGGEGAAAYEEVTYEGYGPGGAAVLVEI
                     MTDNKNRTVAEIRHIFSKHGGNLGENGCVAWMFAKKGSIVIDKKVIDEDALMELVLEA
                     GAEDVKTEDSEYEVITDPGSFEAVKKILDDKGIKHLEARIGMIPSNTVKLEANKAEQM
                     LKMMEKLEDNDDVQNVYSNFDIDEEVMKKLS"
     gene            3548..4033
                     /gene="ruvC"
                     /locus_tag="PLR13_04750"
     CDS             3548..4033
                     /gene="ruvC"
                     /locus_tag="PLR13_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQC18675.1"
                     /translation="MTLRILGIDPGSHVTGYGVIEKKGNYLKHVVHGEIKPKRNSSLS
                     EILVYIYQQLSSLIAQTVPQAVALENIFYGKNVRSLIKQAQVRGVVIFAGADKDLNIF
                     EYSPLEVKKAVVGYGRAEKRQVQVMVKAILNLPETPPPDAADALATAICHANFLKDKP
                     I"
     gene            4033..4641
                     /gene="ruvA"
                     /locus_tag="PLR13_04755"
     CDS             4033..4641
                     /gene="ruvA"
                     /locus_tag="PLR13_04755"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQC18676.1"
                     /translation="MIALISGTIAYKGISQIIVDVRGVGYRVFIPLTTFYELPETGQT
                     ISLHIHTNVKEDAIHLYGFHTLQERDLFQLMISVSGIGPKVAMNILSGIAAPELLRAI
                     SGGNVSKLVNVPGVGKKMAERLVLELKEKVLKKMAIEEAPEDQTQHLADDLMQEDVLS
                     ALMNLGYRNNVARDALNKVLRTSEAKLSMDKILKETLKILAG"
     gene            4664..5689
                     /gene="ruvB"
                     /locus_tag="PLR13_04760"
     CDS             4664..5689
                     /gene="ruvB"
                     /locus_tag="PLR13_04760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQC18677.1"
                     /translation="MEKSKKNSLISPSCAEEENDFEVTLRPTMLKDYVGQEKVKSNLA
                     IFIEASRKRREALDHVLLYGPPGLGKTTLAYIIAREMNVDIKVTSGPVIERPGDLAAI
                     LTNMHENEVLFIDEIHRLSHVVEEILYPAMEDFHIDILIGQGPSARSMKLDIPRFTLV
                     GATTRAGSLTSPLRDRFGMHFRLEFYSPKELAVIIKRSASILGVKLTASGAEELAGRA
                     RGTPRIANRLLKRVRDYAEVKADGTIDGKIAQEALTAFEVDQKGFDQMDRRLLLTLIE
                     KFNGGPVGIESLAAAIGEERVTIEDVYEPFLIQEGYLQRTARGRIACSRAYEHFGLKA
                     SGQTEIF"
     gene            5724..6266
                     /locus_tag="PLR13_04765"
     CDS             5724..6266
                     /locus_tag="PLR13_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HQC18678.1"
                     /translation="MKILMHICCGPCTIYPLKELRTQGHEVTGVFYNPNIHPYQEYKK
                     RYQTLSQYADQIYLKIIWPEDYLMEEFLKGVAFKGKERCSYCLQDRLTFTAQMALQEK
                     YDAFTTTLLYSKYQKHDFIHQAGDNLSQKMGVSFYYQDFRVGWEEGVKISKELEMYRQ
                     PYCGCIYSEKERYYKQNKHS"
     gene            6459..7388
                     /gene="lpxC"
                     /locus_tag="PLR13_04770"
     CDS             6459..7388
                     /gene="lpxC"
                     /locus_tag="PLR13_04770"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQC18679.1"
                     /translation="MNLQRTIKKEINCFSIGLHTGRKVNMKIKPAPADTGIVFIRKDL
                     PEAEPIYARYDNVCDTTLATTLGSNGITVSTVEHLLSAFSGMGVDNAVIELDSFEVPV
                     MDGSALPFVNMLKEVGTHVQKKHKKMLVVKKSVCVNAGDAKAQLLPSEQFKITYEIDF
                     KHPVIGVQSYSMTFSDEEYEKNICAAQTFGFLKDLEFLQARGLALGGSLKNAIVLDDQ
                     KIINKEGQRIPDVFVKHKILDAIGDLFLLGMPIIGHFVAYKSGHRLNNLLLRELISRR
                     DCWEIVTDVNKAENLKSHPFKIPSFRTLDAVQA"
     gene            7548..8117
                     /locus_tag="PLR13_04775"
     CDS             7548..8117
                     /locus_tag="PLR13_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HQC18680.1"
                     /translation="MKKIKNKIGLVLIFLFMGAMFFPSQTCALEPKIKDLLITNDAEN
                     VLLYARLLNGFKPQMELAILAGIPTTFTMWIEVYQERSPMWDKKIIRKEIKRSIKYDN
                     LKKTFSVVSEKKDPDIFSDMESAQKAMSDYNGIVAVPMSSLKKGQSYYTLVKIKMDKV
                     RLPLHMEYVFFFVSLWDFETPWYRQNFTY"
     gene            8171..10390
                     /locus_tag="PLR13_04780"
     CDS             8171..10390
                     /locus_tag="PLR13_04780"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18681.1"
                     /translation="MENKPGRSIIDEKELSKRRRERIIMVVVGVLAIIVTLMASQLFN
                     KENLPLSTNIFVYGLTSINFILIVLLMFLIIRNIVKLFYEHRRGVIGSRLRTKLVAAF
                     VGLSLVPTILLFLFAINFLSYSIDFWFNIKIGDALNRSLEVAQVYYQQMEEHAKFNAR
                     QISADITKNRLYEQDRVEYLKSFVTQRQKNYKAGMVETFFDFQQEKLTFADAEDPDIL
                     PVDPTPKMMENIYLGKEESIIHPAEKGDVIVGIVPVFSYAVPTEVIGRVSVSYMIPKG
                     FVDKLRSIADASEQYRKIELLKKPIKLNYMVTLSIVTLVIIFLAIWFGLTLSKGITGP
                     VQDLVQATNRITQGDLDTRIDIDADDEIGTLVKSFNQMTKDLKKSKTSLEEANLNLEQ
                     RRKYMETVLRNVSAGVISVDKTGIITTINRAAEHMFDIRSEKILFRNYRELMIPEHIS
                     LVDEFLQEMKEKNESVLEKQLEIMLQEKALTVLLTITTIQDEEGNDSGIVVVFEDLTL
                     LQKAERAAAWREVARRMAHEIKNPLTPVQLSAQRLQRKYEDKLGEDGSVFKECTQTII
                     DQVEVLKNLVNEFSRYARMPVTSPSPNDLNAVVSEAVSLFQDAHKDIVFDLKTADGLP
                     KFDLDAEQINRVMINLLDNAVAAMNKKNGHIEIAVSYDEQHRRASVSVADDGPGVPSS
                     YKRKVFEPYFSTKKSGTGLGLAIVSSIISDHHGQVNVSDNSPTGTIVSFQLPVSKAS"
     gene            10531..11901
                     /locus_tag="PLR13_04785"
     CDS             10531..11901
                     /locus_tag="PLR13_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC18682.1"
                     /translation="MKETILVVDDEESICQSLKAILSDEGYQVLVAGSGEEAVKVVEE
                     EMPRLVLLDIWLPGMDGLDTLKAIKKIDAQTMVIIMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKIIILVNNALSLIRLKEENILLKQKVTHQYELTGNSPVIVDLKEMISIVAPT
                     NAWILIMGENGTGKELVARSIHRLSLRADKDIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGNRLIDMDVRV
                     LAATNKDLEEEMKAGRFRQDLYYRLHVIPLVVPPLRDRKEDIPALAERFILDFTRKEG
                     MEPKILSDDAIELLMKHDWPGNVRELKNIIERMIILTPSNDITAKDIPPLNTKEAKSV
                     FPGTPVAAADSFRDAKMDFERQFILKKLEENDGNISRTAEAIGMERSNLHKKLRSLNV
                     VIKGDA"
     gene            complement(11898..12884)
                     /locus_tag="PLR13_04790"
     CDS             complement(11898..12884)
                     /locus_tag="PLR13_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQC18683.1"
                     /translation="MNKKVGFGILISALLVYLSVRGISLQDVLNDLNKIELTYVILFL
                     FLIILMQYLRSYRWGVILQPLEKIDQVSLFSVTSVGFLAIAAIPARMGELARPYLISQ
                     KSSVKMSSALGTIIIERVLDSFTVLGIAILLLFFIDLPSWMIHSSIIFFLLALAIFCF
                     ILFMILRRKETLQLVNKVLAMLPGRLADKIDELLYNFTNGLQIVTNINLLLYLIFLSV
                     TVWLLDVLAIYVLFLAFGFHLPVIASFVLMIILIVGIAIPTAPGFIGNWHFACVLGLS
                     LFNVAKVEALSFAVVYHFLSMIVVVVLGVIFIPFNKFSISEMKNQINNEKQV"
     gene            complement(12916..13320)
                     /locus_tag="PLR13_04795"
     CDS             complement(12916..13320)
                     /locus_tag="PLR13_04795"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HQC18684.1"
                     /translation="MKRNVFLSFISCILIPFLFSGCVPLMVGGAAAGAGAGTYLYING
                     ELKTDYNKSFDQVWKACEKTIAGMHGIDVAPEKEIARGTIQTIINDEKVKFDIAYKAK
                     NLTTVAIRVGVIGNKLSSQLIHDRIADNLVKE"
     gene            complement(13417..14481)
                     /gene="dnaJ"
                     /locus_tag="PLR13_04800"
     CDS             complement(13417..14481)
                     /gene="dnaJ"
                     /locus_tag="PLR13_04800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQC18685.1"
                     /translation="MTKKDYYDILNVPRTASEDEIKKSYRAIAMQCHPDRNPGNKKAE
                     ERFKEAAEAYEVLSDREKREIYDHYGHEGLNSTGFRGFSGFDDIFSHFSNIFDDVFGY
                     GNARGRSHSYATAGADLRYDLTISFLDAAFGTTTAIDLEKLTTCHHCQGTGAAPGSSP
                     EICRTCRGRGQVIQSSGFFTISSTCPHCNGHGKVISTPCENCRGTGKDRIIKTVQLKI
                     PAGVETGSRLRLRGEGETGSQGGPDGDLYVFINVEEHEFFNRSGDDVICRAPISFVQA
                     ALGGTIEVPTLQGKEKMKIPRGTQNGKTFRLRGKGIPHIRGFGRGDQIVEVCVEIPTT
                     LSKKQEELLIEFEKLNQPTR"
     gene            complement(14757..15638)
                     /locus_tag="PLR13_04805"
     CDS             complement(14757..15638)
                     /locus_tag="PLR13_04805"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQC18686.1"
                     /translation="MIKSIQKTDFHELNLISRGKVRDIYSVGNYLLIVTTDRISAFDV
                     IMPDPVPGKGVILNQMSAFWFEKMKDIIPHHIVSTNPAEFPAETAPYRDSLEGRSMLV
                     KKATPLPVECIVRGYISGSGWKDYQRDRTISGITLPAGLKESSRLPEAIFTPTTKAPE
                     GEHDSPISRKEMADIIGAELTASVINISLAIYQRAASMALKAGIIIADTKMEFGLLDG
                     NLMLIDELLTPDSSRFWPADEYKEGHAQKSYDKQFLRDYLLSINWNQKPPAPKLPSEI
                     IENTRKKYEEALKRLVG"
     gene            complement(15654..16283)
                     /locus_tag="PLR13_04810"
     CDS             complement(15654..16283)
                     /locus_tag="PLR13_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18687.1"
                     /translation="MFVKQMQVSSMAVFAYIVGDPITGDALVIDPADNIKGIVAEAQK
                     NNLRINYIVNTHGHVDHISGNTEMQKATGAKIIIHQDDAIMLTHTPAMILRMFGAKAS
                     PPADRLVKEGDILSAGNVELRVIHTPGHSPGGICLYTPGFVFTGDTLFVEAVGRTDLP
                     GGSWQELSRAVREKIFTLPDDTKVMPGHNYGSTPTSTVGHEKRYNPFFR"
     gene            complement(16371..17714)
                     /gene="rsmB"
                     /locus_tag="PLR13_04815"
     CDS             complement(16371..17714)
                     /gene="rsmB"
                     /locus_tag="PLR13_04815"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQC18688.1"
                     /translation="MKKTIRHQAVEILNQVHAAHDFAGPLINDCLDKYNLSGTPDGRL
                     LTHLVYGVLRFRGHLDWLLKKLYRGDFAKMQEDIKNVLRVSLYQLRFSERLPDFAVVN
                     EGVEIAKIINPDKSSLVNAILRNFLRRGQNLSFPSEKKNPVEYLAAFHSHPEWMIQTW
                     IKTFGLQETKALCSADNTLPPLTLRVNTLKISRNDLKEKLAAAGFESQTTPYSPNGII
                     LSTAVKPIQKTEFFDKGLLRIQDEGAQLIACLVNPERNESILDACAGAGGKSSHLAAL
                     LENKGFILAFDRNQEKITELKKEIIRLGITIIETQAGNVSAGLPDAFRERFDCVLVDA
                     PCSGLGTLRRNPEIKWRIMQNDILSFAAQQKKILQQASAAVKRGGRLIYCTCSVLPEE
                     NENIVEHFLRVNNQFSLGKPSSTIIRPLTDSSGFFRTFPHRHNMDGFFGALLIRQ"
     gene            complement(17711..18301)
                     /locus_tag="PLR13_04820"
     CDS             complement(17711..18301)
                     /locus_tag="PLR13_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18689.1"
                     /translation="MFNTPFVKSLLRGISLLLLKMLGWKTSGKIPREPKFVLIVAPHT
                     SNWDLFYGILLAFALKIDARYIAKKELFRGPFSFLMSWTGAIPVERAARHHIVDQMAA
                     LLKEKEKCILALAPEGTRHKTDAWKSGFYYIALKAQVPIQLAFIDYARKTGGTGPLIY
                     PTGHPEHDMSMIRDFYQSVRGKHPENTSTVGIHPES"
     gene            complement(18349..19287)
                     /gene="fmt"
                     /locus_tag="PLR13_04825"
     CDS             complement(18349..19287)
                     /gene="fmt"
                     /locus_tag="PLR13_04825"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQC18690.1"
                     /translation="MTNPRILFMGTPAFALPALRLLHGQRYPIAGVVTQPDRPKGRGL
                     KEIASPVKILAQEFGLNVYQPEKVKDPSFMELLQQLHPDMITVAAFGQILPKSVIEYP
                     RLGCLNIHPSLLPRYRGAAPINWQIINGEKKTGVTIMLMDEGMDSGDILLQEESQLDC
                     EENFGELHDRLAKLGAALLIQTIDQVISGTASRQKQETSGVTFAPRLTRETGKILWTS
                     NASDIVNLIRGLSPLPAAYTFMDGLPLKIFSAVARHENVQQPPGTICAIEADSLSVAT
                     ADGYVIVKDVQLAGKKRMPIADFLRGYHLKIGNSLS"
     gene            19518..20666
                     /locus_tag="PLR13_04830"
     CDS             19518..20666
                     /locus_tag="PLR13_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQC18691.1"
                     /translation="MFLAATFLFGFSQSIVDSTFNNFLNETYLIDNLQRGILEIPREL
                     PGLLVVAVSAIVFFLCSRRQAALANLLCAFGILLIGFYSPNFSVMLIWLFIFSIGQHL
                     FLPLNQSIGMELAVKGQTGRRLGQFSAVANVAMIIGSFSIFVGFQYFDLDFKTSYFAA
                     FLGFSSVAVLIFMMKKDEPVPVRMKFNLRKEYTLYYWLTILYGTRKQLFLTFAPWVLV
                     TVFHQKTQVVATLLTVGCVIGIFFKPMLGKAIDKLGERMILASEAVILVFVCIGYGFS
                     KSIFTETTALYAVFVCYIVDQMLAAVSMARATYLQKIAVRPEDVSQTLTMGVSIDHIF
                     SIGIAVLGGFIWLKFGYQYLFLTGAVIAVLNFFSALQIKTKTEKLTTP"
     gene            20705..21211
                     /locus_tag="PLR13_04835"
     CDS             20705..21211
                     /locus_tag="PLR13_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18692.1"
                     /translation="MKKIKVTVNDLMQQNYVYYLQEPVGKNFHPQFTPDLTPGKMLEM
                     GVFGGKYMTDCKDEFPKRWFTRAKLCHERHDPDLNYFGVTASQPLSVWREKGWIYHED
                     PRGWFQWYCRYYMGRRCSDDERQIKRWKAMKRHISQIRKHCQKGDINCRPRQRQALLN
                     WAYDSRNI"
     gene            21278..21736
                     /locus_tag="PLR13_04840"
     CDS             21278..21736
                     /locus_tag="PLR13_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HQC18693.1"
                     /translation="MTETWLRITNPDNPPNDAMLSAWIGNKAYKYLQQVTKLIVALYP
                     DVFVPEWIYGGKKHGWSLRYKKSKSFCTLIPEKSRFALLIVFGAQERANLETVKDRLS
                     QHTRRDYDRAATYHDGKWLLLPVDNDQVVEDLKILLAVKRKPLTAECIIK"
     gene            21869..22651
                     /locus_tag="PLR13_04845"
     CDS             21869..22651
                     /locus_tag="PLR13_04845"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQC18694.1"
                     /translation="MTINIKTEEHIMLIEIDRPQKYNAMTREMYTQLARAYYRLDRDD
                     DLWVGVVYANGPHFTSGLELTDWSDVFGSGAGFPLSGENEIDPFYMMNEERCRKPLIY
                     AVQGYCFTWGPEVMLNADIRVAASDTKFAMLEVRRGFFPCGGATIRLARDIGWSNAMR
                     YLLTGDQWSAEEAYRMGLVQYVTETGKQLEKAMELARKVASAAPLGVRNILQNARLAE
                     REGEQAAKKVIYQEVSKVMKSEDMKEGLQSFIERRAAVFKGK"
     gene            22708..23280
                     /locus_tag="PLR13_04850"
     CDS             22708..23280
                     /locus_tag="PLR13_04850"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HQC18695.1"
                     /translation="MDPTFFNAGVFKFVILPLLIFSTRICDVTLDTLRIIYVSRGLKL
                     LAASIGFIEVLIWLFAITQIFKNLTNPIYYVAYAGGFAMGNYVGILIEEKMAVGTVVI
                     RTITQKDAGVLIKWLKAEGYGVTYIDAQGAFGPVKVLFTIVKRKDIDHVLGMIRTCNP
                     LAFYTIEDIRSSSKGVFPIVKSRKNRYKEP"
     gene            23356..23559
                     /locus_tag="PLR13_04855"
     CDS             23356..23559
                     /locus_tag="PLR13_04855"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HQC18696.1"
                     /translation="MECKKEKNLDNCNCTYEPCSRKGACCDCLSYHLKQRQLPACCFP
                     KDAEKTYNRSFEYFTELVSQRRI"
     gene            complement(23687..24682)
                     /locus_tag="PLR13_04860"
     CDS             complement(23687..24682)
                     /locus_tag="PLR13_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQC18697.1"
                     /translation="MDHSIKIEDLNDPVMKHASTDFVSIYSDNTVGEALDCIRNCQTQ
                     STILYFYVLDGKARLVGTIQTRRLLTSPLDVKIEEIMSKNLVVIPASATLVEALEFFI
                     FHRYLAFPITDDHEKMLGVIDINVFTEEMIDIEEQGQVHSVFDTLGIKISEVRHKSPW
                     SVFRYRFPWLLATITSGTVCALLVGFFEATLAQSLVLAFFLTLVLGLGESVSMQTMAI
                     TVHSMHHSKAESGWYFQTMQRELTRTFLMALACAFIVGFIVVVWKNDFTAGLIISSGI
                     LTSLLFACFLGVSLPYSLHKLKLDVHVASGPLTLALADICTVIVYFSLAAFILSS"
     gene            complement(25039..26766)
                     /locus_tag="PLR13_04865"
     CDS             complement(25039..26766)
                     /locus_tag="PLR13_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQC18698.1"
                     /translation="MDELSVLITFAVGLAAALFFGYIAVRLKLSPIIGYLLAGIIVGP
                     FTPGVVVNRTIAEQFAEIGVILLLFGIGLRFHLRELIAVWRLAVPGALIQSTLSTSAL
                     AVLLHFMGWDWASGFILGMAISVASTVVMAMVLTEWHDLHAKIGHIAIGWTVVEDILT
                     VALLLLLPIIFSPDRSMDQNIGAVLGLAGIKIVGLLAIVVVLGKWIIPWALERIAKTR
                     SRELFTLAVLVLAVGIAVGSAKLFGVSMALGAFLAGLAVGRSEFAARAGIDALPMRDA
                     FAVLFFVSVGMLFDPLSLIQVPFVIATVLLVVVIIKPLAAALTVRMLGQPLAAAIPIG
                     AAFSQVGEFSFILGTVALQLKIINDAGWNALVAASIISIALNPYVYRWARRLSSKTNK
                     ALPVVKSQPQTVNPSHCILIGYGPIGQIVHNLLTERGNEITVIDLNLKSVRQLNAQGY
                     KAIYGDVLRPGTLEDAGIATAGSLIISTDIEDASEIIKNARLLNPDLQIMVRCAHLRD
                     AAALRQAGATVVAAGEAEVGVALSEVVTAADQMACVMAAEHRETVRRILYDKAFQQNE
                     TVSKKTRKK"
     gene            27204..28136
                     /locus_tag="PLR13_04870"
     CDS             27204..28136
                     /locus_tag="PLR13_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQC18699.1"
                     /translation="MKQFKSILYVTGENGDQQSAIARAVSLAENNQADLTVIDVIPTV
                     MGEYSEEIMSSRMQFLESFTEPYNKRLDIRISVETGTVFIEIIREVLRNKHNLVIKPA
                     ENPDFLKRLFGSTDIHLLRKCPCPVWIMKLPEKSNYSCIMAAVGFKPLETPETEENLN
                     REILDLASSLALSDFASLHVVHAWEAFAAHYIRSRGDKEHEAMAVYVEKERSLHQNAL
                     NVLMEKLSKRVGDDVYQELSPSLHLLQGDAQKMIAALAAKLQADLVVMGTIARTGISG
                     LIIGNTAEAILDQLECSVLAVKPPGFVTPVKLEE"
     gene            28173..28697
                     /locus_tag="PLR13_04875"
     CDS             28173..28697
                     /locus_tag="PLR13_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18700.1"
                     /translation="MKRLSSKSTFFIKRIFPPLWFGCIIFFALMALSAESKGIHVDDI
                     FIVLLAAIFMVLIGYFIMRHLVFDLIDEAYDEGASLLFKNNGQSVRVDLTAIKNVSYT
                     VLINPPRVTLSLRHETQFGDELTFSPSGSFIPFKKNEEILGLIDRIDRARGGSDNTLP
                     IQEGQICKENSTNC"
     gene            28670..29083
                     /locus_tag="PLR13_04880"
     CDS             28670..29083
                     /locus_tag="PLR13_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18701.1"
                     /translation="MQRKFNELLIIGLGGTIFFGSFFAGEYLGASESNKDSWWTPMTM
                     ALSLDQTRPEFELYLKKELLQKHIEKGTLLVANDGENLSKLVLGDIKIRLNNWNKVKA
                     EKLKYAVITAFFLGASIALLIIGLMRFLADKEDAQ"
     gene            29155..29550
                     /locus_tag="PLR13_04885"
     CDS             29155..29550
                     /locus_tag="PLR13_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18702.1"
                     /translation="MKLLRYLWASPYTLLGLFFVPLALLTKGRMKTVDGVLEIHGGFV
                     TWFLKHGLPVRGYIGALTLGHVVLGYNENLLSAYRRHEHAHVKQYEMLGPLFLPVYLA
                     ASIWAWLRGRGAYKGNYLEKKAKEHSREF"
     gene            29624..29995
                     /locus_tag="PLR13_04890"
     CDS             29624..29995
                     /locus_tag="PLR13_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQC18703.1"
                     /translation="MKKTVNKQNLKTEFYMSEKCYITELSNSADDPDVSIARARVEPG
                     VTTRSHRLKGITERYFIISGRGAVEIGELPPQEVNPGDIVLIPPLCPQRITNIGKNDL
                     IFLAICSPRYMQDAYEDVEDF"
     gene            complement(30005..32413)
                     /gene="priA"
                     /locus_tag="PLR13_04895"
     CDS             complement(30005..32413)
                     /gene="priA"
                     /locus_tag="PLR13_04895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQC18704.1"
                     /translation="MYVEIALNIPSDKLFSYEVPTELEQDIVVGKRVFVPFGNKKRTG
                     FITDIRHSCGLNNIKSVAEVLDDEPLFHTSDLNFYKWIAGYFIYPLGKTLAELIPAGP
                     EKKDFLWITPLISETDLHLTPAQNTLMAFLKQHPRGTTLTGIAKISGLKNALSAAKKL
                     QQAGLVSLEKKHGKPLSPQSEKMVALDEQKLPDVKLTGKQRMLVEWMQAKGSVSWGEL
                     IEGSGVTGAVLKALLDKGLIHLTKQEKIRTASFDSHIGRHKNATVLNDRQNAAMEEIS
                     RYLEKNIFTPILLHGVTGSGKTEIYLKAIENVLQTGGSAMYLVPEIALTPQLISRVAG
                     RFDRERIAILHSGIAESVRYDQWRQIRRGRIHLVIGTRSALFAPLTNLRLIIVDEEHD
                     TSYKQDDRLCYNARDLAVVKAKMSSAVVIAGSATPSIHTYFNAQTQKYHYLELPGRID
                     DKPMPVVEIVDMKLQQAKAGKVPVLSDSLIEQIDNTVRNKEQVLLFLNKRGFDTFLVC
                     ADCGYSFRCPNCAVSLKNHLAEGIVKCHYCDYTIQSMPLCPSCKSSRILSYGTGTQKL
                     EKEVQKFFPDARIDRMDSDTTTRKGSQEKILQALDKREIDILIGTQMITKGHDFPFIT
                     LVGVVSADTSLNMPDFRAAERTFQLITQVAGRGGRGSAPGRVIIQTFNPDHYALRHAQ
                     NHDYKSFYREEIKFRLDLQYPPFGRIINLRLSSIKKDVLIEEAKRLGKLAKKLCAGYG
                     NTVEIIGPAESPLSKLRGRFRWQMLIKGKDINALHQIAKDVMHKNKNTQIKMMADVDP
                     ESFM"
     gene            complement(32589..32975)
                     /gene="dksA"
                     /locus_tag="PLR13_04900"
     CDS             complement(32589..32975)
                     /gene="dksA"
                     /locus_tag="PLR13_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQC18705.1"
                     /translation="MEKQINVKQKQEKMKAYKAMLTQKINELLSEAGKTVSEMTNGKE
                     NYPDPTDRASLESDRNFELRIRDRERKLIMKMQEAIKRIDDGTFGICEVCGGPISEKR
                     LMARPVTTLCIDCKTKQEKMEKLKGE"
     gene            complement(33104..33598)
                     /gene="rfaE2"
                     /locus_tag="PLR13_04905"
     CDS             complement(33104..33598)
                     /gene="rfaE2"
                     /locus_tag="PLR13_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQC18706.1"
                     /translation="MNKILDRTTLQRKLDTLRQKGRKIAFTNGCFDILHVGHVRYLRE
                     ASKTGDVLVLALNSDSSVRAIKGEKRPLVPATERAEILAALDFIDFITIFDELTPLDV
                     IKSLKPDILIKGGDWPEEKVVGREEVKKWKGKVVIIPEVQGKSTTNIVEKILQVYGPQ
                     NQKG"
     gene            complement(33616..34359)
                     /locus_tag="PLR13_04910"
     CDS             complement(33616..34359)
                     /locus_tag="PLR13_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQC18707.1"
                     /translation="MNLAEAFNDLSQVIFPPQCLGCAEIIPSVKNQIFCRDCAKHVHF
                     IAGSICPVCGTTFPGSPAENHLCEDCLKKQPYFSQARAVFCYEAVILDAIHQFKYKRD
                     IATGEILADFMADYPFPDIAFDDYSLIVPVPLHVKRLRERGFNQSLILARRLAKRKQI
                     PVDFLVLKRHRFTSTQTQSNKQERKINIRGAFEVRGIKTIAGRNILLIDDVFTTGATV
                     NECSKTLMKAGAKKVSVLTLARVLQHVSF"
     gene            complement(34356..34751)
                     /gene="rsfS"
                     /locus_tag="PLR13_04915"
     CDS             complement(34356..34751)
                     /gene="rsfS"
                     /locus_tag="PLR13_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQC18708.1"
                     /translation="MPAKKNVTRERLILCINAVLQKKAKDIIVLNLSKLSAFTDYMII
                     CSGTSEREVQAIAQAIQLFLKKADIRPLGVEGEANGQWVLLDYDDVVISVFYEPFRTF
                     YDLENLWDAPRMQVDENKKEIKRLSREMA"
     gene            complement(35164..35496)
                     /gene="trxA"
                     /locus_tag="PLR13_04920"
     CDS             complement(35164..35496)
                     /gene="trxA"
                     /locus_tag="PLR13_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQC18709.1"
                     /translation="MNKQDIKHAGDGDFEKTVLKNDKLVLVDFWAPWCGPCRAIGPIL
                     EELAGEYGDRIDIVKVNVDDNPQTASQFGIKSIPTLLVMKGGTVKDTSIGLSSKDKLA
                     DMIKRHIH"
     gene            35636..36316
                     /locus_tag="PLR13_04925"
     CDS             35636..36316
                     /locus_tag="PLR13_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQC18710.1"
                     /translation="MELLKQIQNFGLFQGLSAENLKLMASQALHKKFKQGDLLIGEED
                     PIRAFYVVTAGQLKLYKSSSEGREQTLSLLKPGDPFGLCTAFATESFPANAMAIEKSA
                     VLIIPGELMENVAKKEPVLLLNIIQILSQRLKESMTLIESLSLKEIPARLSTYILDAL
                     SRTSEDKRNKLELTISHRELSKMLGATPEALSRAFKKMSNDKILKVEGRVITVLNREE
                     MKTLAEGD"
     gene            complement(36350..>37016)
                     /locus_tag="PLR13_04930"
     CDS             complement(36350..>37016)
                     /locus_tag="PLR13_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC18711.1"
                     /translation="SWGFSCLIQHEGQNILFDTGGDGLVLAANMAKLGIDPASLDLLM
                     ISHQHWDHTGGIYYILKEKRNLRICLPHSFSARFKEDMKRYGAEVIDINEAREVITGL
                     HSTGDMEALIREQSALLKTQAGTVVITGCAHPGIVHIAETAKRILPDDALALVMGGFH
                     LLDNADEDILKIIARFRELGVRYAAASHCSGPNARELFARKYGEHFIRLGAGSTIALE
                     DLR"
BASE COUNT         9852 a         8291 c         9142 g         9731 t
ORIGIN      
        1 cttattttcc ggaacccctt ttttaatcaa ttttatattt ttgacaatgt gctgttgaat
       61 atttcattga cttacacatt caaattttct ataattttat tagaaatggt ggaaatttta
      121 aaaagtttgc gacgatcatt ggctgattga taaaaacata tctgatattg cgttgcggag
      181 gtcatagtaa tgccgcctgc ggaaatgagg catgaaagag ttgtagtgga aacggaccgc
      241 tatcgcgtgg aaggagatat aacactgcct caggaagggt atcgcagccg cctttccgat
      301 tatttaaacc gaagtgatca ggaatttatc atccttgtaa acgttgaatt gttatcgctt
      361 gacgggtccg gccgcgattg gcgtgccccg gtgcttatgc ttgccagaca gcatatcagg
      421 ctggttgtcc ccgtgggcgg acctaatgct taaggtgaga gaaatcgtga atggccgctc
      481 ttttgcagaa cgttgaaaat cattcctttt aaattcatcc ggcattggaa cagtttatct
      541 gaaacccgta caatgacatt tatcttccat aaaataataa ccgcagatgt tgtaaaaacg
      601 atcatggcag gatcgtaaag aatattttca atgaataaaa aacatgtgaa ggagtttgta
      661 aaatgaacgt caaagatttg tttgatttga agggcaagat ttccgtcgtg acaggcggcg
      721 gcagaggcat cggtaaattc atagcgacgg ggcttgcgga agccggttca gatgtcgtca
      781 ttgcgtcgcg caagatggaa aacctcgaaa aagaagtaaa ggaactggaa aatctgggta
      841 tccgggctct ggcggtgaaa tgcgatttgg ccaaaaaaga agatatcggc aatctggtca
      901 aagccgccat ggataaattc ggcacgatcg acatcctggt caataatgcc ggactgacat
      961 ggggagcgcc gacgctggat tatccgctgg ataagtggga aaaaatattt gacgttaatg
     1021 tcaagggcgt gtggattctt tcccagcagg ttgcccggat tatgaaggaa aaaggcggcg
     1081 gcaagatgat caatgtgtct tccatctacg gttcgcgcgg ctccaatgaa gaagagcatc
     1141 cggccgttgc ttataatccg tccaaatttg ccgtggaagg cttgaccaaa acgctggccc
     1201 ttaaactggc ccgttacaag atttatgtga attgcattgc tccgggtttt ttcaaaacgg
     1261 atatgatggg ccatttattc aagccggaga tgaaacccat tctcgatttg acggtcggcg
     1321 ctattccgct caagatgtac ggggaagaag acgatgtaaa gggattggcc gtatttctgg
     1381 catccaaggc gtccaattac attaccggcg gtattttccc cgtggacggc ggattttccg
     1441 cgcagtaaaa agtccggatg ggtttgcgaa agaggcatga cgccgaagat ttcacgggct
     1501 ttgaaaagtc atgaggtaaa aaacatgaaa aaaataaaac attcgttttt ccttttgctg
     1561 tctttgattg tggtagcctg tgctcccgct tatacgctca atcattcttc accagtcaaa
     1621 cccctcagtc tgcccgctga cgccgccgct cattactggg ctcagaatga atggtggtat
     1681 tacacaggac atttgaaagc ggaggatgga agggaattcg gatatgaatt gaccttcttc
     1741 aagcgaatta ccagtgagga taaggttccg cattttttta ttcccatccc ggcgcactgg
     1801 ctcaaagatc ttggaatgat ggggcatttt gttattaccg acattaccca tcagaaattt
     1861 atgacgcaag aggtgaataa tttcttcggc ttgccgtcaa aagcggacga tacaaaatac
     1921 gatctcatga tcggcacctg gacggcccgt gaagaagaag ggaaacatct tctgtacgct
     1981 gaaatggatg acggccggat cgaactggaa ctgcagccgg taaaggtcgc cgccctgcat
     2041 ggacgacagg gcatcgtcga taaaggaggg ggcaattcca attattatta ttcattgacg
     2101 aacatgaaaa cgtccggcac tttaaccatc gggggcgaat cgcttgatgt gcatggaaaa
     2161 tcatggatgg atcatgaata cggaaccatg aagctcactt acccgcagag cggatgggat
     2221 tggttcagta ttcagctgga gaaccattac gaattaatga tctatgttat cagaagtgaa
     2281 ggcgatctga aaaacagcct ggtcggtgga acctttgttc tgccgaatgg aaaaaccatt
     2341 cccgtcaaag cgcgggatat cgaaatcagg aatctggact actggcacag tgaaaaaacc
     2401 gactccgatt atccgtcttc atgggaaatc attgtcaggc ctttcaagct aaaactgaaa
     2461 tgtcagccgg ttatggctga gcaggaagtc aatttacaac ccatgcccta ctgggaaggg
     2521 gccatatccg tcagcggaac gtttcatggc aaatccgtca aaggtcaagg gtatattgaa
     2581 ctggtgggct attccagaaa atatccaatc caatacatta attaaaatga ttccgccatg
     2641 gaacgataaa cgaaccttta tttttaccat gaaattattc ttccacataa ggcaataggc
     2701 tttacaagcc tattgcctta tgttattaga aaaacgatga aaaaaccatt aaggagaact
     2761 gtatgtccgg ccattccaaa tggagcacga ttaaaagaaa aaagggtgcg atagatgcca
     2821 aacgcggcaa aatatttact aaaattatca aggaaataac tctggcggca aggctgggcg
     2881 gaggggatat tgaaggcaac tcgaggttaa ggcaggctgt catgctggcc aaggaagaaa
     2941 atatgcccaa ggataatatt gagcgggcga tcaaaaaagg taccggcggc ggcgaaggcg
     3001 cggcggccta tgaagaagtc acctatgaag gttacggacc gggcggtgcg gcggttcttg
     3061 tggaaatcat gacggataat aaaaacagaa ctgttgcaga aatcagacat atcttttcga
     3121 agcatggcgg caatctcggt gaaaacggct gtgttgcgtg gatgttcgcc aagaagggga
     3181 gtattgttat cgacaagaaa gtgattgatg aagatgctct gatggaactg gttttagaag
     3241 ccggggcgga agatgtgaaa acggaggaca gcgagtacga agtgattacc gatcccgggt
     3301 cctttgaagc ggtcaagaag attctggatg acaaggggat taaacatctg gaagccagga
     3361 tcggcatgat tccgtctaat accgtaaaac tggaagccaa caaggcggag cagatgctga
     3421 agatgatgga gaaactggaa gataacgacg atgtccagaa cgtatattcc aatttcgata
     3481 ttgatgaaga ggtcatgaag aagttgagtt gatatccata aaaaccattt ttaaaaatat
     3541 ggaatgaatg acattgcgaa ttctggggat agatccgggc agtcacgtca ccggttacgg
     3601 cgtcattgag aaaaaaggaa attatcttaa gcacgttgtt catggtgaaa taaaaccgaa
     3661 aagaaactcg tctttgtcgg aaatcttggt atatatttat cagcagcttt cctctctgat
     3721 tgcgcagaca gttccccagg ctgttgcgct ggaaaatatt ttttacggga aaaatgtacg
     3781 cagtttgatt aaacaggcgc aggtgagggg cgtggtgatt tttgccggcg cggataagga
     3841 tttgaatatt tttgagtatt cgccgctgga agtcaaaaaa gcggttgtgg gctacggcag
     3901 agcggagaag cgacaggtac aggtgatggt taaggctatt ttaaatctgc cggaaacgcc
     3961 tcccccggat gcggcggacg ccctggcaac ggccatatgc catgccaatt ttttaaagga
     4021 caaacccatc tgatgattgc tttaatcagc ggaaccattg cctacaaggg catctctcag
     4081 attattgtag acgtgcgggg cgtcggttat cgggttttta ttcctctgac gactttttat
     4141 gaactgccgg aaaccggcca gaccatttcg ctgcatattc atacgaacgt caaggaggat
     4201 gccattcatt tgtacggttt tcacacgctt caggaaagag acctttttca actgatgatt
     4261 tccgtcagcg gcatcgggcc gaaggttgcc atgaatattc tttccggcat cgcggcgccg
     4321 gaactgctca gggccatctc cggaggcaat gtcagtaaac tggtgaatgt tccgggcgtc
     4381 ggtaaaaaaa tggccgagcg tcttgtcctg gagttgaagg aaaaggttct gaaaaaaatg
     4441 gcaatcgaag aggctcccga agatcaaacg cagcatcttg cggatgatct catgcaggaa
     4501 gatgtccttt ccgctttaat gaatctgggg tatcgaaaca atgttgcccg ggatgcgctc
     4561 aacaaggttc tgcgcacatc cgaagcaaaa ttgagtatgg ataaaattct gaaagagaca
     4621 ttgaaaattc tagccgggta aaaagacgta taaggaatca gcgatggaaa aatcgaaaaa
     4681 aaattccctc atcagtcctt catgcgcgga agaagaaaat gattttgaag tcaccttaag
     4741 accgaccatg ttaaaagatt atgtcggtca ggaaaaggtg aaaagcaatc tggccatatt
     4801 catcgaagcc tccagaaaac gccgggaggc ccttgaccat gtccttcttt acggtcctcc
     4861 gggactcgga aaaacgacgc tggcctatat tatcgccaga gaaatgaacg ttgatatcaa
     4921 ggtcacatcg ggaccggtta tcgaaagacc gggagatctg gcggcgattc tgacgaatat
     4981 gcatgaaaat gaagtgcttt ttattgatga gattcaccgt ctttcccacg tcgtggagga
     5041 aattctgtat ccagccatgg aagattttca catcgatatc ttgatcggac agggaccgtc
     5101 tgcccgatcc atgaaactgg atattccccg attcactctg gtgggggcaa cgacgagagc
     5161 gggatcgctg acctcgcccc tgcgggatcg tttcggcatg cattttcgtc tggaattcta
     5221 ttcgccgaag gaactggccg taatcattaa acggtcggcc tccattctgg gtgtgaaact
     5281 gacggcttcc ggcgccgagg aattggccgg acgtgcgcgt ggcacacccc gtatagccaa
     5341 ccgcttgctc aagcgcgttc gcgattatgc cgaagtgaag gcggacggaa ccatagacgg
     5401 gaaaatagcg caggaagcgc tgactgcttt tgaagtcgat cagaaagggt ttgatcagat
     5461 ggacagaagg ctgctgttga cgctgattga gaaattcaac ggcggtccgg tcggtatcga
     5521 gagtcttgcc gccgccattg gagaagaaag ggtcaccatt gaagatgtct atgaaccatt
     5581 cctgattcag gaaggttatc tgcaaaggac ggcacgcgga cggatcgcct gttccagggc
     5641 ttatgaacat ttcggcctga aggcttccgg acagactgaa attttttaag cggacaacgt
     5701 atcgcaacgg atgttgttgt aagatgaaaa tattaatgca tatttgctgc ggaccctgca
     5761 cgatttatcc tctgaaagaa ttaagaacac agggacacga agtcaccggt gttttttaca
     5821 atcccaacat tcacccgtat caggaataca aaaagcgtta tcagacctta agccaatacg
     5881 cggatcaaat atatttaaaa atcatctggc ccgaagatta cttgatggaa gaatttttaa
     5941 agggcgtcgc tttcaaagga aaagaaaggt gctcttactg tctgcaggat cgtctgacgt
     6001 ttacagctca gatggccttg caggaaaagt acgatgcgtt cacaacaaca ttgttataca
     6061 gcaagtatca gaaacatgat ttcatccatc aagccggaga taatctcagt caaaagatgg
     6121 gagtatcttt ttactatcag gacttccgcg tggggtggga ggaaggcgtt aagatatcca
     6181 aagaactgga aatgtaccgt cagccgtatt gcggttgcat ttacagtgag aaagaacggt
     6241 attacaaaca gaataaacat tcctgagatc aattgttttt tcataatcgc tgtgtcaaat
     6301 ttgccacact ttaatcgaaa taagttggtc ttgctgcgta ttgtctttaa atagttcaat
     6361 aattacaatg atttcttttt taataaatat catcaaatat ggcatgttct ttgcctttga
     6421 taaatttgaa gaaagattag atatcctggc ataaaattat gaacttacaa cgtacaatca
     6481 aaaaagagat aaattgtttc agtatcgggc tgcatacagg gcgaaaggtt aatatgaaga
     6541 ttaagccggc acctgcagat acggggattg tttttatccg caaggatctt cccgaggctg
     6601 aaccgattta tgcgcggtat gacaatgttt gcgacacgac cctcgctaca acgctggggt
     6661 ccaacggtat taccgtttcg acagtggagc atcttttgtc ggcattcagc ggcatgggtg
     6721 ttgataacgc ggtcattgaa ctggattcgt ttgaagtccc ggtgatggac ggaagtgcgc
     6781 ttccgtttgt gaatatgctc aaagaagtgg gaacgcatgt acaaaagaaa cataaaaaaa
     6841 tgctggtggt aaagaaatcc gtttgtgtga atgcgggaga tgcaaaagcg cagttactgc
     6901 cctcggaaca atttaagatc acctatgaaa tcgatttcaa acatcccgtc atcggggttc
     6961 aatcctacag catgactttt tctgacgagg agtatgaaaa aaacatttgt gcggctcaga
     7021 ccttcggatt tttaaaggac ctggagtttt tgcaggccag aggtctggca ttgggcggat
     7081 ctctgaaaaa tgccattgtg cttgatgatc aaaagatcat taataaagaa ggacaaagaa
     7141 tccctgatgt ttttgtgaaa cataaaatcc ttgatgccat cggcgatttg tttttgctgg
     7201 ggatgccgat tatcggtcat tttgtcgcgt ataaatcagg ccaccgtttg aacaacctct
     7261 tacttcggga actgatcagc agaagagatt gctgggaaat cgtcaccgat gtaaacaagg
     7321 cagaaaatct taaaagccat ccattcaaaa ttccttcatt tagaactctg gatgccgttc
     7381 aggcgtaatc caaattcatc ttgcttttta tggtcatgtt gcctagtatt gcacgttgat
     7441 ccgccaaatg agctgaaccg ttatttatcg gttacttgga tcggatcatc gatgctgttt
     7501 aattttgcag ccgcaacggg tcctgctgcg tgggctgatg aattggtatg aaaaaaataa
     7561 aaaataaaat cggtctggta ttgatttttc ttttcatggg cgcgatgttt ttcccgtcac
     7621 agacgtgcgc tctggaaccg aaaattaagg atcttctgat taccaatgat gcagaaaatg
     7681 ttcttttata tgcccgtctg ctgaacggtt tcaaaccgca aatggaactg gcgattttag
     7741 caggcattcc gacgacgttt accatgtgga ttgaagtgta ccaagagcga tcccccatgt
     7801 gggacaaaaa aatcatccgg aaggaaatta aacgatcgat caaatatgat aacctgaaaa
     7861 aaaccttcag cgtcgtcagt gaaaagaagg atccggacat tttttcggat atggaaagtg
     7921 cgcaaaaagc gatgtctgac tacaacggta tcgttgccgt tcccatgtcg tctttaaaaa
     7981 aggggcaaag ctattatacg ctggttaaaa taaaaatgga caaggttcgt ttgccgctgc
     8041 acatggaata tgtctttttc tttgtttcgc tgtgggactt tgaaacgccc tggtacaggc
     8101 aaaactttac ttattaacac ggatcagttt tgtcatgata tgaatgattt caaaaccgtt
     8161 actgtgatct atggaaaata aacctggcag aagcattatt gacgaaaaag aattaagtaa
     8221 acgacgccgc gaacgaatta ttatggtggt cgtcggcgtt ctggccatta ttgttaccct
     8281 gatggccagt cagctgttca acaaggaaaa cctgcctctt tcgacgaaca tttttgttta
     8341 cggcctgacc agtatcaatt tcattctcat tgttctgttg atgtttctca ttatcagaaa
     8401 cattgtcaaa ctgttttacg aacaccgccg cggggtcatc gggtcccgct taagaacaaa
     8461 actggtagcc gcattcgtcg gcctgtcgct tgttccaacc atcctgcttt ttttgtttgc
     8521 gatcaatttt ctttcttaca gtattgattt ctggttcaac attaaaatcg gcgatgcatt
     8581 aaaccgttcg ctggaagttg ctcaggttta ttaccagcag atggaagagc atgccaagtt
     8641 caacgcccgt caaatcagtg cggatataac aaaaaaccgc ctttacgaac aagatcgtgt
     8701 tgaatatctc aaaagcttcg tcacgcaacg tcaaaaaaac tacaaagcgg ggatggtgga
     8761 aacatttttc gatttccagc aggaaaaact gacgtttgcc gatgcggagg atccggatat
     8821 actgccggtg gatcccacgc cgaaaatgat ggaaaacatt tatctgggca aggaagaatc
     8881 aatcatccat ccggcggaaa agggcgatgt gattgtcggc atcgtgccgg tattttccta
     8941 tgcagtgccg acggaagtca tcggccgggt gtcggtgagc tacatgatcc ccaaggggtt
     9001 tgtcgataaa ttaagaagca ttgccgatgc gtcggagcaa tatcgcaaaa tcgaactgct
     9061 gaaaaaaccc attaaactca attatatggt cacgctgtcg atcgtgacgc tggtgatcat
     9121 atttttggcc atctggttcg gtttgacatt gtcaaaaggc attaccggcc ctgtgcagga
     9181 tctggtgcag gcaaccaaca gaatcacgca aggagacctg gacacccgca ttgatattga
     9241 cgcggatgat gaaataggga cgctggtgaa atcattcaat caaatgacga aagatttgaa
     9301 aaagagtaaa accagccttg aagaagccaa tctgaatctg gaacaacggc gcaaatacat
     9361 ggaaaccgtg ttgcgcaatg tttccgccgg ggtgatttcc gtggataaaa cgggtatcat
     9421 tacgacgatc aaccgtgcgg cggaacacat gtttgacatc cggtcggaga aaattctgtt
     9481 tcgcaattac cgggaactga tgattcccga acacatatcg ctggtggatg aatttcttca
     9541 ggaaatgaag gaaaaaaatg aaagcgtgct ggaaaaacag ctggaaatca tgctgcaaga
     9601 gaaagcgctg accgttttgc tcacgatcac aaccattcag gatgaggaag ggaatgattc
     9661 cggtatcgtc gttgttttcg aggatttgac attgctgcaa aaagcggagc gcgctgcggc
     9721 atggcgtgaa gtggcccggc gcatggcgca tgaaattaaa aatcctctga cgccggttca
     9781 actctccgca cagcggcttc agcggaaata tgaagataaa ctcggggaag acggttccgt
     9841 gttcaaagaa tgcacgcaga ccattatcga tcaggtggag gtattgaaaa atctggtgaa
     9901 tgaattctcg cgttacgcga ggatgccggt tacctcgccg tctcccaatg atttgaatgc
     9961 tgtggtctcc gaagccgtct cgctgtttca ggatgcgcat aaagacatcg tctttgattt
    10021 gaaaacagcc gatggtttgc cgaaattcga tctggatgca gagcagatca accgagtcat
    10081 gatcaatctt ctggataacg ctgtcgcggc gatgaataag aaaaacggac acattgaaat
    10141 cgccgtcagt tatgatgaac aacatcgccg tgcttctgtt tccgtcgccg atgacggtcc
    10201 gggagtacct tccagctata aacggaaggt ctttgagcct tatttttcca ccaagaaatc
    10261 gggaaccgga ctggggttgg ctattgtcag ttcgatcatt tccgatcatc atggtcaggt
    10321 gaatgtcagc gataacagcc ccacgggaac gattgtctct ttccaactgc cggtttcaaa
    10381 agcgtcgtga gactcacgtc tgataaggcc cgggcagcgg aaacagtcac cggaagattg
    10441 cggaacgtcg cagcgcatgt acccgaaggc ttgcgttacg gttcattccc gtgatatata
    10501 aaaaaaagtt tacgcgtaga aagagtgagc atgaaggaaa ccattcttgt tgttgatgat
    10561 gaagaaagca tctgtcagtc cctgaaagca attctcagcg atgaggggta tcaggttctg
    10621 gttgccggca gcggtgaaga agccgtaaaa gtggtcgaag aggagatgcc gaggctcgtt
    10681 cttctcgata tctggcttcc ggggatggac ggtctggaca cgctgaaagc catcaagaaa
    10741 atcgatgccc agaccatggt cattatcatg tccggtcacg ggacaattga gacagccgtg
    10801 aaagcgacaa aactcggcgc tttcgatttt attgaaaagc ctctttcgct cgataaaatc
    10861 atcatcctgg tcaataatgc cttaagtctg atccgtctta aagaagaaaa cattttatta
    10921 aaacaaaaag taacccatca gtatgaactt accggaaaca gccctgtgat cgtggatctc
    10981 aaggaaatga taagcattgt tgcgccgacg aatgcctgga tcctgatcat gggtgaaaac
    11041 ggaacaggta aagaactggt ggcgcgttcg attcatcgtt tgagcctgag agcggacaag
    11101 gatattgttg aggtgaactg cgcggcgatt ccggaagaac tcatcgaaag cgaattattc
    11161 gggcacgaaa aaggcgcctt caccggggcg acggaaaaga aacgcgggaa atttgatctg
    11221 gcgcacgaag gcacgatctt tctggatgaa gttgccgaca tgagtttgaa ggcgcaggcc
    11281 aaaattttac gcattctgca ggagaaaaaa ttcgagcggg tcggcggcaa cagattgatc
    11341 gacatggatg tgcgcgttct ggccgcaacc aataaggatc tggaagagga aatgaaagca
    11401 ggccgtttcc gtcaggatct ttattaccgc ctgcacgtca ttccgctggt tgttccgccg
    11461 ttacgcgaca gaaaagaaga tattccggcg ctggctgaaa ggtttatcct ggattttacc
    11521 cggaaggaag gtatggaacc gaaaatactc agtgatgacg ccatcgaact gctgatgaaa
    11581 catgactggc cgggaaatgt ccgcgaattg aaaaatatta ttgaacgaat gattattctg
    11641 accccctcca acgacataac cgccaaggat attccgccgc tcaatacgaa agaggcaaag
    11701 agcgtatttc ccggtacacc ggttgccgcc gctgattctt tcagggacgc caaaatggat
    11761 tttgaacgac agtttattct gaaaaaactg gaagaaaacg atggaaacat ttccagaacc
    11821 gcggaagcca tcggtatgga acgcagcaac ctccataaaa agctcaggag cctcaatgtg
    11881 gtcatcaagg gggacgctta aacctgtttt tcgttgttga tctggttttt catttcggaa
    11941 attgaaaact tgttgaaggg tataaagata acgcccagca ccaccacaac gatcatggaa
    12001 agaaaatgat aaacgacagc aaacgaaagc gcttccactt ttgccacgtt gaagaggctt
    12061 aaccccaaga cacaggcaaa gtgccagttg ccgatgaaac cgggcgccgt cggaatggct
    12121 atccccacaa tcagaataat cattaaaaca aacgaagcga ttaccggaag atgaaatccg
    12181 aaagccagaa aaagtacata aatggccagc acgtccagca gccagaccgt gaccgaaaga
    12241 aagatcagat ataaaagcag gttgatattc gtgacaattt gcaacccgtt ggtgaagttg
    12301 tacagcaact cgtcgatttt atcggccagc cggcccggaa gcatcgccag tactttgttg
    12361 accagttgaa gggtttcttt cctgcgcagg atcataaaaa gaataaaaca gaatatcgcc
    12421 agcgccagca aaaagaaaat aatgctggaa tggatcatcc atgaaggcaa atcgatgaaa
    12481 aagagaagaa ggatggcaat gcccaggacg gtaaagctgt ccagaactct ttcaatgata
    12541 atggtaccca gcgcggacga catttttaca gaactttttt gggaaattaa atatggtctt
    12601 gccagttccc ccatccgtgc cgggatggcc gcaatcgcca gaaagccgac cgatgtgacc
    12661 gagaaaaggg agacctggtc gattttctcc aggggttgca gaataacgcc ccagcgatag
    12721 gaccgcagat actgcattaa aatgatcaga aaaagaaaga ggataacgta ggtgagttca
    12781 attttattca gatcattcaa aacatcctgc agactgattc ctctgacgga caagtagacc
    12841 agaagcgcac tgatcagaat gccaaagccg acttttttgt tcatgaattt acccggccat
    12901 tatgctcaca agccatcatt ctttgaccag gttgtccgca attctgtcgt gtatcagttg
    12961 agaagaaagc ttgtttccaa taacgccgac gcgaatcgcc accgtggtta ggttctttgc
    13021 cttgtacgca atatcgaatt tgactttttc atcgttgatg atcgtctgaa tggttccccg
    13081 ggcgatttct ttttccggtg ccacgtcaat gccgtgcatt ccggcgattg ttttttcaca
    13141 ggccttccat acctgatcga acgatttatt gtaatccgtt ttgagttctc cgttgatgta
    13201 taaataggtg ccggctcccg caccggcggc cgctcctccg accatcagcg gtacgcatcc
    13261 ggaaaaaagg aaagggatca atatgcatga gataaaggat agaaaaacat ttcttttcat
    13321 gtgtcttcct cccgtgattc gttggcggta tttaaagcac atgtcgtcgg tgattgcaac
    13381 ggcaattatg gggaaacagg gggtgccgca gaaaagtcag cgagtgggtt gattcagttt
    13441 ttcaaactcg attaaaagtt cttcctgctt tttactcagc gttgtgggaa tctccacaca
    13501 cacttcgacg atctgatcgc cgcgtccgaa cccgcggatg tgcgggatgc ctttgcccct
    13561 gagcctgaag gttttgccgt tttgtgttcc tctgggaatt ttcatttttt ccttgccctg
    13621 cagcgtgggc acttcgatgg tgcccccgag cgccgcctgc acaaaggaga tcggcgcccg
    13681 gcaaatgacg tcatctcccg acctgttgaa aaactcgtgc tcttccacgt tgatgaaaac
    13741 ataaagatcg ccgtcgggac cgccttgact gcctgtttcc ccttctccgc gcagacgcag
    13801 acgcgaaccg gtttcaacac cggcgggaat tttcaattga acggttttga tgatccgatc
    13861 ttttcccgta ccccggcagt tttcgcaggg ggtcgatata accttgccat gcccgttgca
    13921 gtgcggacag gtggagctga tggtaaaaaa gccgctggac tgtatcacct ggcctcgtcc
    13981 cctgcaggtg cggcagattt ccggggagga cccgggcgcc gctcctgtgc cctgacaatg
    14041 atggcatgtg gtcagttttt ccagatcaat tgctgtggtg gttccgaaag cggcatccaa
    14101 aaaagagatg gtcagatcgt aacgcaaatc ggcgccggca gtggcgtacg aatgagaccg
    14161 cccccgagca ttgccgtaac cgaaaacgtc atcaaagata ttgctgaaat gagaaaaaat
    14221 atcgtcgaac ccgctgaagc ccctgaatcc ggtgctgttt aatccttcgt gaccgtagtg
    14281 atcgtaaatt tctctttttt ctctgtcgct taatacttcg taggcttcgg cggcttcttt
    14341 aaatctttcc tccgcctttt tattgccggg gtttctgtcc ggatggcact gcatcgctat
    14401 tgcacggtag ctttttttta tttcgtcctc ggaagccgtt ctcgggacat tcagtatgtc
    14461 gtaataatcc ttcttcgtca tgcgcttttt aagtcaacct cgttttgtat tattttttta
    14521 agagcctcaa tttctgtcct gttgtctgtt ttctgcatgg cctgaagagc acgggagaaa
    14581 ttttattgtt cgattggttt ttcgccgatt ctttcccggt ctgagggttt tacgtccatg
    14641 tgagggcttg gccatctgct gaaagagcag gacatctccg ttgccaaacg cgacatctct
    14701 gattttatgc ttgcgtgtaa tttaacaatc aattggcgtt cgtcaatatt cgaatatcac
    14761 ccgaccaatc gtttcagggc ctcctcgtat ttttttcttg tgttttctat gatttccgaa
    14821 gggagtttag gcgccggcgg cttctggttc cagttaatgg aaagcaggta atcccttaag
    14881 aattgtttat cgtaactttt ttgcgcatga ccttctttgt attcgtcggc cggccagaaa
    14941 cgcgaagaat cgggagtcag cagctcgtca atcaacatta agttcccgtc caggaggccg
    15001 aattccattt tcgtgtcggc aataatgatt cctgccttca gcgccatgga ggctgctctc
    15061 tgatagatcg ccagggaaat atttattacg ctggcggtca gttcggctcc gataatatct
    15121 gccatttctt ttctgctgat cggcgaatca tgttctccct ccggcgcttt ggttgtcggc
    15181 gtaaaaatcg cttccggtaa tcgggaagat tctttcaacc cggccggtaa tgtgatgccg
    15241 gagatagtcc tgtctctctg ataatccttc cagcccgagc cgctgatata gccgcggaca
    15301 atacattcca ccggaagggg tgtggctttt tttaccagca tgcttcttcc ttccagactg
    15361 tcgcggtagg gagccgtctc tgccggaaac tcggcggggt tggtggagac aatgtgatgg
    15421 gggatgatat ctttcatttt ttcaaaccag aaagcggaca tctgattcag aatcacgccc
    15481 ttgccgggga cgggatccgg cataataacg tcaaaagcgg aaatgcgatc ggtggttaca
    15541 atcagcagat aattaccgac ggaataaata tcgcgcacct tgccgcgact gatcagattc
    15601 agttcgtgga agtctgtttt ctgtatgctt tttatcataa aaataaattc cttttagcgg
    15661 aaaaatggat tgtatctttt ttcatgtccg acggtggagg tcggtgtgct gccgtaatta
    15721 tgtccgggca tgactttggt atcatcgggt aaggtaaata ttttttccct gacggctctg
    15781 gataattcct gccacgagcc gcccggtaaa tccgttctgc cgacggcttc cacaaaaagc
    15841 gtatctccgg taaagacaaa gccgggcgtg taaagacaga ttcctccggg agaatgcccc
    15901 ggtgtatgaa tcaccctgag ttcgacgttg cccgcggaaa ggatgtctcc ttccttaacc
    15961 agcctatcgg ccgggggaga tgcttttgcg ccgaacattc tcaagatcat ggccggtgta
    16021 tgcgtcagca ttatggcgtc gtcctgatgg atgataattt tcgcgcccgt tgctttctgc
    16081 atttccgtat tgccgctgat gtgatccaca tggccgtgcg tattgacgat ataattaatc
    16141 cgcaaattat ttttttgagc ttccgccacg atgcctttaa tattatccgc cggatcgatg
    16201 accagtgcgt ctcccgttat cgggtcgcct acaatatagg caaataccgc catggaactg
    16261 acctgcattt gtttaacgaa catggcttgt ctccctggaa aaaagtgact ggctaataac
    16321 attatttgac gtaaaacgtc aatcgaattt tattctataa attgttgaat ttattggcgt
    16381 atgagtaagg ctccgaagaa tccatccatg ttgtgtctgt gcggaaatgt ccggaaaaat
    16441 ccgctgctgt ccgtcaacgg acggatgatg gtcgaagaag gcttacctaa agaaaattga
    16501 ttatttaccc ttaaaaaatg ctccacgata ttttcatttt cttccggaag gaccgagcag
    16561 gtgcaatata tcagccggcc tcctcgttta acggcagcgg aggcctgttg cagaatcttt
    16621 ttttgttgtg ccgcgaagga gaggatatcg ttttgcatta tgcgccattt gatttccgga
    16681 ttgcggcgca gcgttcccag tccggagcag ggtgcatcca ccagaacaca atcgaatcgt
    16741 tccctgaagg catccggcag accggcgctt acattgccgg cctgcgtttc gatgatggtg
    16801 attcccaatc ggatgatttc ctttttcagc tcagtgattt tttcctggtt gcgatcaaac
    16861 gcaagaatga aacctttgtt ttcaagaagg gccgccagat gagaggattt gcctcccgcg
    16921 cctgcgcatg catcgagaat ggattcattt ctttcgggat tgaccaggca ggcaatcaac
    16981 tgcgcccctt catcctggat gcgcagtaat cctttgtcga aaaattctgt tttctggatc
    17041 ggcttcacgg cggtgcttaa aatgattcca ttcggggaat agggggtggt ttgggattcg
    17101 aagccggccg ccgcaagttt ttcctttaaa tcgttgcgcg atattttcaa cgtgttgacg
    17161 cgcagcgtca gcggcggcag tgtgttgtcg gcagagcaca gcgcctttgt ctcttgcaga
    17221 ccgaatgttt tgatccatgt ctgaatcatc cactccggat gcgaatgaaa ggcggccagg
    17281 tattcgaccg gatttttctt ctcggaagga aaggatagat tctgaccgcg gcgcagaaag
    17341 tttcttaaaa tagcgttcac cagcgaactt ttgtcgggat tgattatttt ggcaatttca
    17401 accccttcat tgaccacggc aaaatccggc aaccgctcgc tgaatctgag ctgatacaga
    17461 cttacgcgca aaacattttt gatgtcttct tgcatttttg caaagtcgcc gcgatacagt
    17521 tttttcaata accagtccag atgaccgcgg aaacgcagaa cgccgtaaac aagatgggtg
    17581 agaagacgcc cgtcgggggt tccggataaa ttgtatttgt caagacaatc gttgatcaat
    17641 ggaccggcaa aatcatgggc ggcatggacc tgatttaaaa tctcgacggc ctgatggcga
    17701 atagttttct tcatgattcg ggatggatac ccaccgtgct tgtattttcg ggatgtttcc
    17761 cccggacgct ctgataaaaa tcgcggatca tgctcatatc gtgctccgga tgcccggtcg
    17821 ggtatatcag cggtcccgtc ccgccggttt tgcgcgcata atcaataaat gccagctgga
    17881 tgggaacctg cgctttgagc gcgatataat aaaaccctga tttccaggca tccgttttgt
    17941 ggcgggtgcc ttccggcgct aaagcaagga tgcatttttc tttttctttc agcagggcgg
    18001 ccatttgatc gacgatatgg tgacgcgctg ctctctcaac cggaatggct cccgtccagc
    18061 tcatcaaaaa ggaaaaagga ccgcggaata attctttctt cgcaatatac cgggcgtcga
    18121 ttttcagggc aaatgccaga agaattccat aaaacaaatc ccaattggag gtatgcggcg
    18181 cgacgatcag gacgaacttt ggctcccggg ggattttccc ggatgttttc cagcccagca
    18241 ttttcagcag cagcagggag attccccgca gcaaagattt tacgaaaggc gtgttaaaca
    18301 tggtatggtt cataaaagcc ccaggccttc ttcataaaaa attttatttc agctcagcga
    18361 gtttccaatc ttcaggtgat agccgcgcag gaaatcagca atgggcattc ttttctttcc
    18421 cgccagttga acgtctttta cgatcacata gccgtcggcg gttgcgacag acaaactgtc
    18481 ggcttcgatc gcgcagatgg ttccgggggg ctgctgtacg ttttcatgac gggcaaccgc
    18541 actgaaaatt ttcaggggga gtccgtccat gaatgtgtag gcggcaggca aagggctcag
    18601 cccccgaatg agattgacaa tgtccgatgc gttgctggtc cagagaattt ttcccgtttc
    18661 tcttgtcaac cgcggtgcga acgttacgcc ggaggtttcc tgtttttgcc ggctggcggt
    18721 tccgctgatc acctggtcaa ttgtttgaat cagcagcgca gcgcccagtt tcgcgagacg
    18781 atcgtgaagc tcgccgaaat tttcttcgca gtccagttgt gattcttcct gcagaagaat
    18841 atcgccggaa tccattcctt cgtccatcag cataatcgtg accccggttt ttttttcgcc
    18901 gttgatgatc tgccagttga tcggcgccgc gccgcgatac cggggcagca gggaagggtg
    18961 gatattgagg caacccagcc gcggatattc tataaccgat ttgggcagaa tctgtccgaa
    19021 cgccgcaacc gtgatcatgt ccggatggag ttgctgcagc aactccataa aagacggatc
    19081 ttttactttc tccggttgat aaacgttcag accgaattcc tgagccagaa tcttgaccgg
    19141 cgaggcaatt tctttcagcc ctcttccttt cgggcggtcg ggctgggtga caacaccggc
    19201 aatcggatag cgctgtccat gcaggaggcg caaagccgga agagcgaatg cgggcgtgcc
    19261 cataaaaaga atgcgagggt tggtcatcgg gaaaatatcc tttatttttt taatgtaaac
    19321 acatatacaa aatcatcggg attgctcttc ctgtccgcac atggcgggat ggctcaattg
    19381 caaatttcga tatacgttgt tttcgaaatt ttagtctcat aatccctgca aggtgtcaag
    19441 gattacgaat tattaggggt aagattatca ttactcgcac taaacatacc ctcgcgaaat
    19501 taccgcggga tttccgtttg tttctggcgg cgacctttct gtttggcttt tcgcagagta
    19561 ttgttgattc cacattcaat aattttttga atgaaacgta tctgatcgat aatctgcagc
    19621 ggggcatttt ggaaattccc cgcgaattgc cgggacttct ggtcgttgcg gtttccgcga
    19681 ttgtattttt tctgtgttca cggagacagg cagcgctggc caatctcctg tgtgccttcg
    19741 gcattctact catcggattt tattcgccga acttttccgt tatgctgatc tggcttttca
    19801 tcttcagtat cggacagcat ttgtttctgc cgttgaacca gagcataggc atggaactgg
    19861 cagtcaaggg acaaaccggc aggaggctgg gacagttcag tgccgttgcc aatgtggcaa
    19921 tgattatcgg cagcttctcc atctttgtcg ggtttcaata ttttgatctg gacttcaaga
    19981 catcatactt tgcggctttt ctcggattca gttccgttgc ggttttgatc ttcatgatga
    20041 agaaagacga accggttccc gtgaggatga aattcaacct gcgcaaggaa tatacgcttt
    20101 actactggct gaccattttg tacggaacac gcaaacaatt gtttctgacc tttgcgccct
    20161 gggtgctggt aaccgtcttt catcagaaaa cgcaggtggt ggcaacgctg ctgaccgtcg
    20221 gctgtgtgat cggcatattt ttcaagccca tgctcggcaa ggcgatcgac aaactgggcg
    20281 agcggatgat tctcgcgtca gaggcggtta tactcgtttt tgtctgcatc ggttacggtt
    20341 tttcgaaaag tatttttacg gagaccacgg cgctctacgc agtgtttgtc tgttacattg
    20401 tcgatcagat gctggcggcg gtatcgatgg cgcgggcgac gtatctgcag aaaatagccg
    20461 tcagaccgga agatgtttcg caaacgctga ccatgggcgt cagtattgat catatctttt
    20521 ccattggcat agcggtgctc ggcggtttta tatggctgaa gtttggctat cagtatttat
    20581 ttttgaccgg tgcggtgatt gccgtgctga attttttttc cgccttgcag atcaaaacaa
    20641 aaacggaaaa actgacgacc ccttaatatc aaagtggtca agaacattct gaggagatca
    20701 acacatgaag aaaataaaag taactgtcaa tgatttaatg cagcagaatt atgtttatta
    20761 tcttcaggag ccggtcggga aaaactttca tccgcaattt acccctgatt taacgccagg
    20821 aaaaatgctg gaaatgggcg tattcggcgg aaaatatatg accgattgca aggatgagtt
    20881 tccgaaacgc tggtttactc gcgcgaagct ttgtcatgaa cgacatgacc ccgatctgaa
    20941 ttatttcggt gtaaccgctt cacagccttt atccgtctgg cgtgaaaagg gctggatcta
    21001 tcatgaagat ccccgtggct ggtttcaatg gtattgccgg tattatatgg gccggcgatg
    21061 ttccgatgat gaacggcaga ttaaaagatg gaaagcgatg aagagacaca tttctcaaat
    21121 caggaaacat tgtcaaaaag gcgatatcaa ctgccgtccc agacaaaggc aggcgctgct
    21181 gaactgggct tacgatagca gaaacattta gggatcgagt tgtaatcatt attttcacag
    21241 actaaaacgc acagaccatt tcttttatga gggatatatg acggaaacat ggttacgaat
    21301 aaccaatccg gacaatccgc cgaatgatgc tatgctgagt gcatggatag gcaataaagc
    21361 gtacaagtac ttgcagcagg ttacaaaact cattgtggcg ctatatcccg atgtctttgt
    21421 gccggaatgg atctatggcg ggaaaaagca cggctggtcg ctccgctata agaaaagcaa
    21481 atcgttttgc accttgattc cggagaagag tcgttttgcg ctgctcattg ttttcggtgc
    21541 acaagaacgg gcaaaccttg agaccgtcaa agacagactt tcgcaacaca cccgaaggga
    21601 ctacgatcgg gcggcaacgt atcacgacgg gaaatggctg cttctccccg tcgataatga
    21661 tcaggtcgta gaagatctga aaattcttct ggctgtaaaa cgcaagcctc tgacggcgga
    21721 atgcataatc aaataacctg ataaattatc ttgagacacg tggtataccc atgcatggct
    21781 cctttgcaat cgcccgaatt tcttccgttg acacaagtag tccctttccc tatactccta
    21841 acattactat cttaaaggag taccttccat gacgatcaat attaaaacag aagagcatat
    21901 tatgctcatt gaaatcgacc ggcctcaaaa atacaatgcc atgacgcgcg aaatgtacac
    21961 gcagctcgct cgggcctatt acagactgga tcgcgatgat gatttatggg tcggggtggt
    22021 ttatgccaac ggaccgcatt ttacctccgg tctggaactg accgactggt cggatgtctt
    22081 tggcagcgga gcgggtttcc cgttgagcgg agaaaatgaa attgatcctt tttatatgat
    22141 gaatgaagaa cgttgccgaa aaccgcttat ttatgcggtg caggggtatt gcttcacatg
    22201 ggggccggaa gtgatgctca acgcggatat tcgcgtggcg gcatccgata ccaagtttgc
    22261 catgctggaa gtgcgccgcg gatttttccc gtgcggtggc gcaacaatcc ggttagccag
    22321 agatatcggc tggtccaacg ccatgcgcta tcttttaacc ggcgaccagt ggagtgccga
    22381 agaagcctat cggatggggc tggttcagta tgtcaccgaa accggcaagc aactggaaaa
    22441 ggcgatggag cttgcgcgaa aagtagccag tgccgcaccc ttgggtgtgc gtaacatttt
    22501 gcaaaacgcg cgcctggcgg aaagagaagg agaacaggcg gcgaaaaagg ttatttatca
    22561 ggaagtatcc aaagtgatga agagcgaaga tatgaaggaa ggcctgcaat ccttcatcga
    22621 acgccgcgca gcggttttca aagggaaatg atccatccgt tcaatgatgt gatgcgataa
    22681 cttctgaaga aaatgcgagg aagataaatg gatccgactt tttttaatgc cggagtattc
    22741 aaatttgtta ttctgcccct gctgattttt tccacgcgta tctgcgatgt gacgctcgat
    22801 acgttacgca ttatttatgt gtcgcgtgga ctaaaattat tggcagcgtc catcggattt
    22861 attgaagttc tgatctggtt gtttgctatt actcaaattt tcaagaatct gacaaatcct
    22921 atttactatg tcgcgtatgc gggcggtttt gcgatgggca attatgtcgg gattttaatt
    22981 gaagaaaaaa tggcagttgg cactgtcgtg atccgaacca ttacacagaa ggatgctggg
    23041 gtattaatta aatggctaaa ggcggaggga tatggcgtta catacataga cgcccagggc
    23101 gcttttggtc ctgtaaaagt tctttttaca attgtaaaaa gaaaagatat tgatcatgtt
    23161 ctggggatga tccgcacgtg taatccgttg gcattttata ctattgaaga cattcgttcc
    23221 tccagcaagg gagtattccc gattgttaaa tccaggaaga acagatacaa agaaccgtaa
    23281 cagaagcgct gagaataaaa gtgtttaatg tgaagaaata ttttcagaaa aagttagtcg
    23341 tgaaggatag agtcaatgga atgtaaaaaa gaaaaaaatc tcgataattg caattgcacc
    23401 tatgagccct gcagcagaaa gggggcctgt tgtgactgcc tgtcttatca cttgaagcag
    23461 aggcaactgc ccgcctgctg ctttccgaag gacgcggaaa aaacctacaa caggtctttt
    23521 gaatatttta ccgaactggt atcgcagcgc cggatataaa aagcatgatg ttcctgcaag
    23581 ggaagcacgt gaagtcttat cgggaggcag gtatttatga tgccgctgac gatgtttgtt
    23641 gaaatcgccg caattttgag tttcacaatc gtgatcttca ctggaattat gacgataaaa
    23701 taaacgcagc cagggaaaag taaacaatca ctgtgcagat atcggccagc gccagcgtca
    23761 ggggtccgga agcgacgtga acgtcaagtt ttagtttgtg caaggaataa gggaggctca
    23821 cacccaaaaa acatgcaaag agcaacgacg ttaaaattcc tgatgaaatt atcaggccgg
    23881 ccgtaaaatc gtttttccat acaacaacga taaatccgac aataaacgcg caggccaaag
    23941 ccattaaaaa cgtgcgcgtc agttcccttt gcattgtttg gaaataccag ccggattctg
    24001 ctttactatg atgcatggaa tgaaccgtta tggccatggt ctgcatggag acgctttcgc
    24061 ccaggcctaa aaccagcgtc agaaagaaag ccaaaaccag gctttgcgcc agcgtggcct
    24121 cgaaaaaacc caccagcagc gcgcaaacag tgccgctggt aatggtagcc aaaagccagg
    24181 ggaagcgata acggaaaacg ctccagggcg acttgtgtcg cacttcggaa attttaatac
    24241 caagagtatc aaacacgctg tgcacctgtc cctgctcctc gatatcaatc atttcctcgg
    24301 taaacacgtt gatatcgata acccccagca ttttttcatg gtcatcagtg attggaaacg
    24361 ccagataacg gtgaaaaata aaaaattcca gcgcttccac taaggttgcg ctggcgggga
    24421 ttacaacaag attttttgac atgatttctt caatttttac atccaacgga gacgtcagga
    24481 gtcttcttgt ctggattgtt ccgaccaaac gggctttgcc atcaagaaca taaaaataaa
    24541 gaatggtgga ttgtgtctgg cagttgcgga tacaatccaa tgcctcaccc acggtattgt
    24601 cgctatagat agacacaaaa tctgtgctgg cgtgtttcat cacggggtca ttgagatctt
    24661 ctatttttat ggaatgatcc atgcgatgcc tttcctgtat agaatcagaa taacctcaat
    24721 gttgaatata tacggctttt ttaactaaca caaaccattt catttatcag atattaatca
    24781 tgattaatac ggctcatcag aacgctgaag aatgatatag cttttaagta taaaattaag
    24841 aagcggaaaa gtccctgtga caatcttctg cgccttatgg aattgcatga caacagtgac
    24901 gctatcgtct gagatggcga aacggggttg acgatgctct ttcccgatgg atgcggaaaa
    24961 aacttacaac aggtcttttg aatattttag ccaaccggta tcgcagcacc ggatgtagaa
    25021 aaaagccgtt ttcaaatgtt atttctttct tgtttttttc gaaaccgttt cgttctgttg
    25081 aaatgctttg tcatatagaa tacgtcggac ggtttctctg tgttcagcag ccataacgca
    25141 ggccatctga tcggctgccg tgaccacttc tgacagggca acgccgactt cggcttcacc
    25201 ggccgcaacc acggtagcgc ctgcttgacg cagagcggct gcgtcacgga gatgagcgca
    25261 acgcaccatg atttgcaaat caggattgag aagacgcgcg ttcttgatga tctcggaagc
    25321 gtcttcaatg tcggtgctga taatcaggct gccggctgtt gcgatgcccg catcttcgag
    25381 cgttcccgga cgcagaacat caccgtatat ggccttgtat ccctgggcgt tcagttggcg
    25441 aaccgatttc aggttgaggt cgataaccgt gatctcgttg ccgcgttccg tcagaagatt
    25501 gtgaacgatt tgtccgatgg gaccgtagcc gatgaggatg cagtgactgg gattaactgt
    25561 ttgcggttga cttttgacca ccggaagggc tttatttgtc tttgaagaca accggcgtgc
    25621 ccagcggtag acataaggat tcaacgcgat cgagatgatg gaggcggcga ccagcgcatt
    25681 ccatccggca tcgttgatga ttttgagttg aagagcgact gtgccgagaa taaaactgaa
    25741 ttctccaacc tgcgaaaagg cggctccgat ggggatagcc gcagccagag gctggccaag
    25801 catccgaacc gtcaaagcgg cggcaagcgg cttgataata acgaccacaa ggagcacagt
    25861 tgcgataacg aaaggcacct gtataagact cagcggatcg aacagcatcc cgacggaaac
    25921 gaagaagagc actgcaaaag cgtcgcgcat cggcagggcg tcgatgcccg cccgcgcggc
    25981 aaattcggaa cgccccacgg ccagaccggc aagaaaagcg ccaagggcca tcgaaacgcc
    26041 aaacagtttt gcggaaccga cggcaatccc aacggccaga accagcacgg caagcgtaaa
    26101 gagttcccgg gaccgtgttt tggcgatgcg ttccaacgcc catggaataa tccatttccc
    26161 gagaaccacg acaatagcca acagtccgac aatctttatt ccggccaatc cgagaacggc
    26221 tccaatgttc tgatccatgc ttctgtccgg ggagaaaata atgggcagaa gaagcaggag
    26281 tgcaacggtg agaatgtctt ctacaacggt ccagccgatg gcgatgtgcc cgatcttggc
    26341 gtgcagatca tgccattccg tcagtaccat ggccatgact accgtgctgg ccaccgaaat
    26401 agccatgccc aggatgaatc cggatgccca gtcccagccc atgaaatgca gcaagacagc
    26461 cagggcggat gtggacaagg tgctttgaat caacgcgccc gggacggcta aacgccatac
    26521 cgcgatcagt tcacgcagat ggaagcgcaa tccgattccg aagagcagca ggatcacgcc
    26581 gatttccgca aactgctccg caatggtccg gttgacgaca acaccgggag tgaaaggacc
    26641 gacgataatg cctgccagca gataaccgat gatgggagat aatttcagac ggacggcgat
    26701 atatccgaag aaaagagcgg cagcaagtcc aacagcaaag gttatcagaa cgctcaattc
    26761 atccatcgct tatcccttta agaaaaaatg tttgaaaaag acatgatgtt gttatttccc
    26821 gcattagagg cgtcaatgct gtgactgaga aaagataaaa aaacactaca atctaactat
    26881 aataatttat aagaaaaatc aattttttta agtcgttatc aaacggtttt cttgtacata
    26941 cggcgcgcat ctccggaaat gttcattgac atgcatggtt atgttaacta tatttgtaca
    27001 aaagatggaa tttgttcttc tcatgcacac gctgctgcga cgcaatacac attgatcatg
    27061 attcattaga tggtatagat catttctcga ttacgataaa actttcggac cgcgcagata
    27121 tccagcgatc ccggtcattc ttttaataaa gctctgttga ccgctgaaaa atctggaaag
    27181 ggcaataatg aaaggagtga tatatgaaac aattcaaaag cattctctat gtcacgggag
    27241 aaaacggcga tcagcaatcc gcgattgcgc gcgccgtgtc actggccgaa aacaatcagg
    27301 ctgatttaac cgtcatcgat gttattccca cggtgatggg tgaatattcc gaagaaatca
    27361 tgagtagccg gatgcagttt ttggaatcct tcaccgaacc ttataataaa cggttggata
    27421 ttcgaatcag tgtggaaacg ggtacggttt ttatcgagat catccgggaa gtgctgcgca
    27481 acaaacataa tctggttatt aagccagcgg agaatcccga tttcctgaaa aggttattcg
    27541 gaagcacgga tattcatttg ctgcgcaaat gtccatgccc ggtctggatt atgaagttgc
    27601 cggaaaaatc gaattacagc tgtattatgg cggctgttgg ttttaagccg ctggaaactc
    27661 cggagacgga agaaaatctt aaccgggaaa ttctcgattt agccagttct ctggctctgt
    27721 ccgattttgc gtctctgcat gtggttcacg cctgggaagc atttgccgcc cactacataa
    27781 gaagccgcgg cgacaaggag cacgaagcca tggcggtgta tgttgaaaaa gagcgctctc
    27841 tccatcagaa cgcactgaat gttcttatgg agaaactgag caaacgtgtg ggtgatgatg
    27901 tttaccagga gctttcgccg agtctgcacc ttctccaggg agatgcacag aaaatgatcg
    27961 cggcgttggc agccaagctc caggcggatc tcgtcgtgat gggcacaatt gcccgcaccg
    28021 gtatttccgg actcatcatc ggcaacactg cggaagctat tcttgaccaa ctggaatgtt
    28081 ctgtgcttgc ggttaaaccg cccggatttg tgacgccggt caaacttgaa gaataatgcc
    28141 ggtcccgtca gcggatccag agggagggca gattgaaaag actttcatcg aaaagcacat
    28201 tttttattaa aagaatattc cccccgctgt ggttcggatg catcatcttt tttgcgctga
    28261 tggcattgtc tgccgagtcg aaaggcattc atgtagacga tatcttcata gtattgcttg
    28321 ccgctatttt catggtactg atcggctact tcatcatgag gcatctggtg tttgatctga
    28381 tagacgaggc atacgatgaa ggggcgtcgc ttctttttaa aaataacggg caatcggttc
    28441 gtgtggattt aaccgccatt aagaacgtca gctatacggt gctcatcaat cctccaaggg
    28501 tgacgttgag cctgcgccat gagacgcagt tcggtgatga attgactttc tctccgtccg
    28561 gcagtttcat tccgttcaag aaaaatgaag agattctggg actgattgac cgcattgaca
    28621 gggccagagg cggatcagac aacactttac cgattcagga ggggcaaata tgcaaagaaa
    28681 attcaacgaa ctgttgatta tcggtttggg cggaacaata tttttcggtt cattttttgc
    28741 cggagaatat ctgggcgcct ccgaaagcaa caaagatagc tggtggacgc cgatgaccat
    28801 ggctctttcg ctggatcaaa cacggccgga attcgaactt tatcttaaaa aagaactgct
    28861 gcaaaagcac atcgaaaaag gcactctcct ggttgccaat gacggggaaa acctgtcaaa
    28921 attggttttg ggtgatataa aaatccggct caataactgg aacaaggtga aggcggaaaa
    28981 attaaaatat gctgttataa ccgccttttt tttaggagcg agcattgcgt tgctgatcat
    29041 cggtttgatg cgatttcttg ctgataaaga agatgcacag tagcggttga atccttatat
    29101 ggagaaaccc aaatttacga taatcatttt tcacaacaag gcacctctca accaatgaag
    29161 cttttgcgct atctgtgggc atcgccgtat actctcctgg gacttttttt tgttccgctt
    29221 gcgttattaa cgaagggcag gatgaaaacg gttgacggcg tactcgaaat ccatggagga
    29281 tttgttacat ggtttctcaa acacggcttg cctgtccgag gatatatcgg agcgttgaca
    29341 ctgggacatg ttgtgctggg atacaacgaa aatcttctat ctgcgtatcg ccgtcacgaa
    29401 cacgcgcatg tcaaacaata cgaaatgctt gggccgttat tcctgccggt ttatctggcg
    29461 gcatcgatct gggcgtggct cagaggcaga ggggcatata aaggaaatta tctggaaaaa
    29521 aaggcgaaag agcacagccg tgaattttaa aaaactcttt taagatgatg tcggaaagtg
    29581 tatttccttt caggaatatc tgtgcggaga aaataattaa acaatgaaaa aaacggttaa
    29641 caagcaaaat ctgaaaaccg agttttatat gtcggaaaaa tgttacatca cggaattatc
    29701 caattctgcc gacgatcctg atgtatccat tgcccgggca agggtggagc cgggcgtgac
    29761 aacgcgcagt catcggctga aaggaattac ggaacgttat tttatcatca gcggcagggg
    29821 agctgtggaa atcggagaac tgccgcccca ggaagtcaac cccggcgata tagtcttgat
    29881 tccgccctta tgtccgcagc gcattaccaa catcggcaaa aacgatttga tttttctggc
    29941 catctgctcg ccgcgttata tgcaggatgc gtatgaagat gtcgaagact tttaaccctg
    30001 atgattacat gaagctttcc gggtccacat ccgccatcat ttttatttgc gtgtttttat
    30061 ttttatgcat aacgtctttt gcgatctgat gcagggcgtt gatgtctttc cctttgatca
    30121 gcatctgcca gcggaagcgc ccccggagct tggaaagggg agattccgcc ggaccgataa
    30181 tttctaccgt gtttccatac cccgcgcaca gcttcttcgc cagtttgccc agacgtttgg
    30241 cttcctcaat caatacgtct tttttgatcg aagacaggcg caaattgatg atccggccga
    30301 acggcgggta ttgcagatcc agacgaaatt ttatttcttc cctgtaaaag gatttataat
    30361 catggttttg agcgtgcctc agcgcatagt ggtcgggatt gaacgtttga ataatgactc
    30421 ggccgggtgc gcttccccgt cctcctcgtc cggcgacttg cgtgattaac tgaaacgttc
    30481 tttcggcggc gcggaaatcc ggcatgttca gggaagtgtc cgcggaaaca acgcccacca
    30541 gggtaatgaa cggaaagtcg tgtcctttgg tgatcatttg cgtgccgatg agaatgtcaa
    30601 tttccctttt gtccagtgcc tgaagaattt tttcctgaga ccctttacgg gtggtcgtgt
    30661 cggagtccat tctgtcaatc cgggcgtcag gaaagaattt ctgcacttct ttttccagtt
    30721 tctgcgtgcc cgttccgtaa cttaaaatgc ggctgctttt gcaggaaggg caaagcggca
    30781 tcgattgaat cgtatagtcg cagtaatggc atttgacgat gccttccgca agatgatttt
    30841 tgagggagac ggcgcaattg ggacagcgga aactatatcc gcagtcggcg caaactaaaa
    30901 atgtatcaaa cccgcgcttg ttgagaaaaa gcagaacctg ttctttgttc cggacggtat
    30961 tgtcgatttg ctcaatcaaa ctgtccgaaa gcaccggcac ttttcccgct ttcgcctgct
    31021 gtaatttcat atcaacaatc tcgacgacag gcatcggttt gtcatcgatt cttccgggaa
    31081 gttccagata atgatatttt tgggtctgcg cgttgaaata cgtatgaatg ctcggcgtag
    31141 cggaaccggc gatgacaacg gcggaagaca tttttgcctt cacgacggcc aggtcccggg
    31201 cattatagca gagccggtca tcctgcttgt aagaagtatc gtgttcttca tccacgatga
    31261 tcaatctcaa attggtcagc ggagcgaaaa gagcggaacg ggtgccgatt accagatgaa
    31321 tgcgaccgcg tctgatctgc cgccactggt catagcgtac ggattcggcg atgccgctgt
    31381 gcagtatggc aatgcgttcc cggtcaaacc gtccggcaac ccgcgaaatc agctgaggcg
    31441 tgagggctat ttccggtacg agatacatgg cagagccgcc ggtttgtaaa acattttcaa
    31501 tggcttttaa atagatttcc gttttcccgc tgccggttac accgtgaagc agtatgggcg
    31561 tgaaaatatt tttttccaga tagcgtgaaa tctcttccat tgccgcattc tgccggtcgt
    31621 tgagaacggt tgcgttttta tggcgtccga tgtgcgaatc gaatgaagcg gttcggattt
    31681 tctcctgttt cgtcagatgg atcagacctt tatccagtaa ggctttgagc acggcgccgg
    31741 taacgccgga accttcaatc aattctcccc atgataccga gccttttgcc tgcatccatt
    31801 cgacaagcat tcgctgtttg ccggttaatt tgacgtcggg cagtttttgt tcgtcgagcg
    31861 caaccatttt ttcgctttgc ggggacaacg gtttgccgtg ttttttctcc agagagacca
    31921 atcccgcctg ctgaagtttt tttgccgccg aaagagcgtt tttcaggccg ctgattttcg
    31981 cgatgccggt gagcgtcgtg ccccgcggat gttgtttcag gaacgccatc agtgtatttt
    32041 gtgcaggcgt taaatgcaga tcggtttccg atatcagcgg ggttatccag agaaaatctt
    32101 ttttttccgg tcctgccgga atgagctcgg ccagggtctt acccagcgga taaataaaat
    32161 agccggcaat ccatttgtaa aagttcaggt cgcttgtatg aaacagcggc tcatcgtcaa
    32221 gcacttccgc aacggatttg atattgttca ggccgcagga atgacggata tcggttatga
    32281 atccggtgcg ttttttattg ccgaaaggaa caaagacgcg tttgccgacg acaatgtcct
    32341 gctccagttc agtgggaact tcatacgaga agagtttatc cgatggaatg tttaaggcga
    32401 tttctacata cataactatt ccatgaagtt caaaaatata ccgttagtaa ccgatcctgt
    32461 ccgggaaatc catttttctg ataccatctt tagtccaaaa aggaaattat ttatcggtag
    32521 ctattgtttt aaaaataaaa aaacgctccg gttggggagc gttttttatc aagcggaata
    32581 ttttaaattt attcgccttt gagcttttcc atcttttcct gttttgtttt acaatcgata
    32641 cagagggtcg tgaccggtct tgccatcaat cttttctctg atatgggacc gccgcagact
    32701 tcgcagatac cgaaagtgcc gtcatcgatc cttttaatgg cttcctgcat tttcatgatc
    32761 agtttgcgtt ctctgtcccg gatacgcaat tcaaaatttc tgtcggattc aagagacgcc
    32821 ctgtcggtgg gatcgggata gttttctttc ccgttggtca tttccgaaac agttttgccg
    32881 gcttcactca gcaactcgtt gattttttga gtcagcatgg ctttgtaagc cttcattttt
    32941 tcctgtttct gttttacatt tatttgtttt tccaaaatca tcactccttg caagaacaac
    33001 ttggccgtca aatctacatg atgaaaattt atttgtaaag agaaattttt gtccgcagtt
    33061 ttttcaggca atatgcctca attcaatgat agtatgaata aaatcaaccc ttttggtttt
    33121 gcggaccata aacctgcagg atcttttcca caatgttggt ggtggattta ccctgcactt
    33181 cgggaataat gacaactttg cctttccatt tcttaacttc ttcgcggccg acaacttttt
    33241 cttccggcca gtctcctccc ttgatcagga tgtccggctt cagggatttg atgacgtcca
    33301 gaggcgtcaa ttcatcaaat attgtaataa agtcgatgaa gtcgagcgcg gcaaggattt
    33361 ccgctctttc tgttgccgga acgagaggtc ttttttctcc tttgatggct ctgactgacg
    33421 aatcgctgtt taaagccaga accagcacat cgccggtttt gcttgcttcg cgcaggtagc
    33481 ggacatgacc gacatgcaga atatcaaaac atccgttagt gaaagcgatt ttccttcctt
    33541 tttgccgcag tgtatccagc tttctttgca gggttgtcct gtctaaaatt ttattcatta
    33601 ccgctccttt acgttttaaa atgagacatg ttgtaacact ctggccaggg tcaatacgga
    33661 aacttttttt gctccggcct tcatcagggt tttggaacat tcattaacgg tggcgccggt
    33721 agtaaagacg tcgtcgatga gcaggatatt gcgtccggca attgttttta tccctctgac
    33781 ttcaaaagct cctctgatat ttatctttct ttcttgttta ttcgactgag tctgggttga
    33841 cgtaaatctg tgccgtttca acaccaagaa gtcaacagga atttgttttc tcttcgccag
    33901 acggcgggcc agaataagcg attgattgaa ccctctttcg cgcagtcttt taacatgcag
    33961 aggaacggga acaatcagcg aataatcgtc aaaagcaatg tccggaaaag gataatcggc
    34021 catgaaatcc gccagaattt cccctgtagc gatatcccgt ttgtatttaa attgatgaat
    34081 cgcgtcgagg atcaccgctt cgtaacaaaa gacggccctc gcttgagaaa aataaggctg
    34141 tttcttcaga caatcttcgc acagatggtt ctccgccgga ctgccgggaa aggttgtgcc
    34201 gcaaacggga cagatgctgc cggcaataaa atggacgtgt ttagcgcaat cccgacaaaa
    34261 aatctgattc ttcacggacg ggatgatttc agcgcatccc agacattgag gcggaaaaat
    34321 tacttggctt aaatcattga acgcttcagc cagattcatg ccatttctct gcttaacctt
    34381 tttatttcct ttttgttctc gtcaacctgc atccggggag catcccagag attttcaaga
    34441 tcgtaaaacg ttctgaacgg ttcataaaaa acggaaatga caacgtcgtc gtaatccagc
    34501 agaacccatt ggccgttggc ttcgccttcg acgcccagag gtctgatgtc cgcttttttc
    34561 aggaataatt gaatggcctg ggcgatagcc tggacttccc tctccgacgt tccgctgcag
    34621 atgatcatgt aatctgtgaa agctgaaagt ttgcttaaat ttaaaacaat gatgtctttt
    34681 gctttttttt gtaatacagc gttaatacac aaaatcaggc gttctctggt cacgtttttc
    34741 tttgctggca aataaacctc ctgtccggca ctgaattttc cgaatatatg ccacaaatga
    34801 cggaaaatga caactttgct gtttttttgc atgaagatta taaaaaatcg tcatggggta
    34861 ttcaggacag tttctgtttt ctttgcacat gtttttacac aggatgaacc ccttgcgtcc
    34921 gattcgagaa acaggccctg acgttgttta tgaaacaata tttacccgtt taaagcagtt
    34981 tctgctttcg tgttaagaat cggttgatgt tttttctttc tttctgtcct cgaccggaca
    35041 gccgggtttc attcaggtgg atacacccaa tttaggcaag agccatccct gcaacaaaaa
    35101 ccataccgca acaatgacgg ccaatatcaa aaaatcattc atttcctttg ccccctggtg
    35161 ttatcagtgg atatgccgtt taatcatatc ggctagttta tccttcgagg acaaaccgat
    35221 cgacgtgtcc tttaccgttc cccctttcat gaccagcagt gtcgggatgc ttttgatacc
    35281 gaattgactc gcggtttgag gattgtcatc cacgttaacc ttcacaatgt ctatccggtc
    35341 gccgtattct ccggccagtt cctccagaat cgggccgatc gcacggcaag gtccgcacca
    35401 cggcgcccag aaatccacta aaaccagctt gtcatttttt aaaacggttt tttcgaaatc
    35461 accgtcaccg gcatgcttga tgtcctgctt attcatttgc tcccctccct tgatataaaa
    35521 gtatttgatt tatcaatccg caattgttaa atattcgtat attcataatc gcaagcatat
    35581 gtcccggttt gtggaatgtc attgatctga atcaatgtgg agaaaaatat ttgccatgga
    35641 acttcttaaa caaatacaga actttgggct gtttcaaggc ctgtcggcgg agaacttgaa
    35701 actcatggca tcgcaggcgt tgcataaaaa attcaaacag ggtgatttgc tgatcgggga
    35761 ggaagatccc atccgggcgt tttacgtggt gaccgccggg cagttaaagc tttacaaaag
    35821 ttcatccgag ggcagggaac agacgttatc gcttttaaaa ccgggagatc ctttcggtct
    35881 ttgtacagcc tttgccacgg aaagctttcc cgccaatgcc atggctattg aaaaaagcgc
    35941 agtattgatt attccgggag aattgatgga gaatgtcgct aaaaaagaac cggtgctgct
    36001 tctcaacatc attcaaatac tttcccagcg cctcaaggaa tccatgacgc tgatcgaatc
    36061 gctttcgctg aaggaaatac cggcaaggct gtcaacctac attcttgacg cactttcaag
    36121 gacgtcagaa gacaagagaa acaaactgga attaaccata tcccaccggg aattatccaa
    36181 gatgcttggc gcaacgcccg aggcgctttc ccgtgcgttc aagaaaatga gcaacgataa
    36241 aattctgaag gtcgagggac gcgtcattac cgttttgaac agggaagaga tgaaaacgct
    36301 ggccgagggc gattaagtta ttccagtgct aaacattgat gatccggggt tatctcaaat
    36361 cctccagagc gatcgtactg cctgcgccga gacgaatgaa atgctctccg tatttgcggg
    36421 cgaagagctc gcgggcatta ggaccgctgc agtgagaggc ggccgcgtaa cggacgccga
    36481 gttcccggaa ccgggcgatg attttcaaaa tgtcttcgtc agcattgtcg agcaaatgaa
    36541 aacctcccat gaccagggcc agtgcatcat ccggtaaaat acgcttcgcg gtttcggcaa
    36601 tatgcacgat gcccggatgc gcacatccgg taatcacgac ggttcccgcc tgcgttttca
    36661 gaagggcgct ctgttcccgg atcagggcct ccatgtcgcc ggtactgtgc agaccggtga
    36721 tcacttctcg tgcttcattt atgtcaatca cttctgcgcc gtagcgcttc atatcttctt
    36781 tgaagcgggc ggaaaacgaa tgaggcagac aaatccggag atttcgtttt tctttcagaa
    36841 tataatagat gcctccggta tgatcccaat gctggtgaga gatcatcaga agatcaagcg
    36901 atgccggatc aatgcccagc tttgccatat ttgccgccag gacaagacca tcgccgccgg
    36961 tatcaaaaag gatgttttgc ccttcatgct ggatcagaca ggaaaatccc cacgag
//
LOCUS       DAONQZ010000018        33557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1430_length_33557_cov_16.150618, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000018 DAONQZ010000000
VERSION     DAONQZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 33557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33557
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_33557_cov_16.150618"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLR13_04935"
     CDS             <1..615
                     /locus_tag="PLR13_04935"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQC18712.1"
                     /translation="STFIEPLACVVRAQRLARVKKGQTVLVIGCGMSGLLNVKLAVTK
                     GCRIIASDINRKKLDIALEMGAHIAINAADDISKRLVDQTGRKADIVFLCASADAAVE
                     QAWQCVDKGGVIVLFAVPAPDKKVVVPVNDFWMKEIAILTSYYCGPSDIIEAMKLIEW
                     HHVAIDDLITHRLPLTEIVKGFQLVADGRESIKVIIKPQEQAAL"
     gene            complement(622..1308)
                     /locus_tag="PLR13_04940"
     CDS             complement(622..1308)
                     /locus_tag="PLR13_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18713.1"
                     /translation="MNLKPIYRLFAVVMIFSVFCLSGCAGPRLYSVGMYYDAEQSVIP
                     AYVKNDGQTSDAVVAVSEFADTRQIDDPLVIGRVIQNSGEKTLVFPRNVRATRAVANG
                     IKQYLKKAGYKITEINEPWNLKEDKMPMGKAKILIGGNIEELEIICRKGKLTNSYDTT
                     MTLAVVIADMGQGRILYKTRVKSSYSREHILFSENILGQQADKVLSDTIEKLFEDQKV
                     AQTIKDVMVR"
     gene            1473..2126
                     /locus_tag="PLR13_04945"
     CDS             1473..2126
                     /locus_tag="PLR13_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18714.1"
                     /translation="MTEATQQALTGLRDLSMIKWYVIPLLAIVFYIYTKEIKDARQNG
                     DWNAVLAGLTIFGVDFFNETWNGWVMYLTQRSAFWTTPGDTALRTLVGWNIEIMFMFL
                     IIGIIFYHTLSESKKDKILGLPEKWFWAISYSIFCVFVECLLNIGGHLVWEYPFWYLS
                     FKGVWLIFLIGYFHFFCFAILVISLKSIKAKLTTVGIIYAAPIIMNVLAFGFWGWNY"
     gene            complement(2130..2666)
                     /locus_tag="PLR13_04950"
     CDS             complement(2130..2666)
                     /locus_tag="PLR13_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQC18715.1"
                     /translation="MKPIGPLMWEHRLIEKMLTAMMRHMDQVEKTRKVNPLVIDMAVD
                     FIRVYADRTHHGKEEEILFRDLAKKDLPADLKNIMQELLDEHVWGRKTTAALVEAKNK
                     YLQGNQAELFRLIDTARQLGNFYPKHIEKEDKHFFYPCQEYFNKEEQTKMLDEFWEFD
                     RKMIHEKYNKIYDDYSGL"
     gene            2935..3879
                     /locus_tag="PLR13_04955"
     CDS             2935..3879
                     /locus_tag="PLR13_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQC18716.1"
                     /translation="MMLLKRTPLLILLCLVLIFSCQQAKDPGRNDKKLNVIATIFPVY
                     DFARHIGGDKITLAMLLPPGTDAHHYELKPDDIIKVTNADVFLFTNFELEQWAYKIIG
                     ASDKNTRLQAVETGNGAMMLRLNKEEDDHEAHPAGFDPHIWLDMDNAQKMVDNITSAF
                     IKKDPRNSDYYLKNAGDYKRQLADMDQKYRTTLAACKTRTVLHAGHWAFAYLTSRYHL
                     KYIAAYNVFADAEPAPQKIFALIDQIKNENVSHVYYEDMMNPRLAKTIARETGTGLLK
                     LNNGHDVTRTDLEKGETFLALMEKNLVNFQKGLQCPEK"
     gene            3864..4613
                     /locus_tag="PLR13_04960"
     CDS             3864..4613
                     /locus_tag="PLR13_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQC18717.1"
                     /translation="MSGEIIKVDHLSFCYNGTKVINDISFTVQRGDYLGIAGSNGSGK
                     STLIKNILGILEPHQGHITIFDQPVASFDQWHRIGYLPQRINALHYHFPCTVAEIVQL
                     GIKKRNPDDLKRTLDLMGIAHLSSCLIGELSYGEQQRVMLARALIRKPDLLIFDEPTT
                     ALDPETRDVFYALTAKANEHGTTVVLVTHDTGTIGKYARNLLYLDKKIIFFGTFRDFC
                     ASPDMTGFFGPNSQHMICHQHDQKGPATPWN"
     gene            4604..5431
                     /locus_tag="PLR13_04965"
     CDS             4604..5431
                     /locus_tag="PLR13_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQC18718.1"
                     /translation="MELSGIFQYAFVQRAFLGGILIAAIASILGVFLVLRRFSLIGDG
                     LSHVTFGSVAVVMLLGISPAHMTLAALPLVMLSSLAILKLTGSKRIQGDAAIGIISSF
                     GIAAGIILTSISGGYNLDLFSYLFGNILTVTLTEIFFAIIVFLTVVATVIILYDDLLA
                     ITFDEELARTMGVQTGKINTVLFLLTAVAAVLAMKVAGIMLVSAMLIIPPLTALQLSL
                     SFRMTIFTSIIFSVLSVILGITMAFLLNLPAGGTIVVLNIFFLLCVFFARKLMAFGR"
     gene            5438..6364
                     /locus_tag="PLR13_04970"
     CDS             5438..6364
                     /locus_tag="PLR13_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18719.1"
                     /translation="MTLPFQAYRSSIIFIIALFCGMVFDEGRQAGRILILPALMIILT
                     ITLLRFPRGFFRHPASLLFSSIRGNLLNYLVLGNFILLTGAFLIQKQELWIGLALVAA
                     MPASLEIILLGILLRIEKNYVFTSISGTYLGALLIVPLVSLCFFKYIQINYWNIAVLI
                     LCLIALPLVLSRLIVEKDWDRILRKHENTLIDFCHFIVFYAISANSSMFLKKLSDDLV
                     FISLIALTSTFLFYFLIRRIGLYFHGQENKVNFFILLGTMKDYGLAGGIALTLFNPEV
                     AVPPLVFAVFTFIYMNWLSFKARRIEDDCGDV"
     gene            6539..7129
                     /locus_tag="PLR13_04975"
     CDS             6539..7129
                     /locus_tag="PLR13_04975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQC18720.1"
                     /translation="MAITLPELPYAKDALAPVISANTLDFHYGKHHKAYVDNLNKLIA
                     GTDLDQKSLEEIINIAAKDSAKAGIFNNAAQVWNHTFYWKCLKKTGGGRPSGAIAAKI
                     NEMWGNYDKFVEELKNAGATQFGSGWVWLVSDKTRLQITKTANADTPLAHGQKALLTI
                     DVWEHAYYLDYQNRRPDYLSAVIQNLINWDFVNANL"
     gene            complement(7214..7495)
                     /locus_tag="PLR13_04980"
     CDS             complement(7214..7495)
                     /locus_tag="PLR13_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQC18721.1"
                     /translation="MNKSDLIEALSTKLNLTEKKAIDVVNLIFKGFTDELKKNGRIEI
                     RGFGSFVVKKYGSYTGRNPKTGSKIQVEPKKLPFFKVGKELKVRVDKKK"
     gene            complement(7607..8335)
                     /locus_tag="PLR13_04985"
     CDS             complement(7607..8335)
                     /locus_tag="PLR13_04985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQC18722.1"
                     /translation="MKERLQKVIAAAGITSRRHAEELITRGRVSVNGVVVTKLGEKAD
                     AQKDIIRVDGKIISTEKVKYYIVLNKPAGFVTTLHDPQNRPTVVDLLGSVPERVYPVG
                     RLDYDSKGLLLLTNDGDFAQKIQHPRFQKPKIYKVKIQGHLSRQDVQQLSRGINVDDD
                     FFKPENLKVDKMNDRSCWLRLTLKEGKNRIIRRGFEKIGYRVALLVREAIGDLALGTL
                     KEGEWRHLTRKEISQLLDSAVKSK"
     gene            complement(8332..8997)
                     /gene="scpB"
                     /locus_tag="PLR13_04990"
     CDS             complement(8332..8997)
                     /gene="scpB"
                     /locus_tag="PLR13_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQC18723.1"
                     /translation="MEKIKAVIEALIFASDTPLSPERIRAVFPEVEKTEIREIIDQLV
                     SEYHDRQGGILLQEVAGGFQFRTNPDLSLWIKKLKTAKPQSLSPQALETLAIIAYKQP
                     IVKSEIEDIRGVDVGGPLKGLLEKKLVRIVGRKDVPGKPIIYGTTRKFLEVFNLKDIM
                     DLPNIRELKELHQQQELLDPDQESSESEQETIVEEEAVAKQHDDENAEENAEEKIEEV
                     PDL"
     gene            complement(8998..9774)
                     /locus_tag="PLR13_04995"
     CDS             complement(8998..9774)
                     /locus_tag="PLR13_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQC18724.1"
                     /translation="MDNNNLGHPMDPMDNEQRLNYAIKLDIFEGPLDLLLYLIKKNEI
                     DIYNIPIALITEQYFQYLKMIKALNLDLAGEYLVMASTLIHIKSRTLLPPPEEPEEEE
                     EDPRAELVRQLLEHKTFKEIAVSLGNRPLLERDVFTRAAALPEVEVKSTDEEEEFIEV
                     SVFELIEAFHRIISQMDKKELMEIDLEKLSLTDIINEIMEQLTVTKNITFEDLLKGKK
                     DRRRIIYTFLAILELIKLRMIKAYQTSMFGVIRIFPAVES"
     gene            complement(9784..10773)
                     /gene="trpS"
                     /locus_tag="PLR13_05000"
     CDS             complement(9784..10773)
                     /gene="trpS"
                     /locus_tag="PLR13_05000"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQC18725.1"
                     /translation="MERKRILSGMRPTGKLHLGNLHGALRNWIALQESGQYDCFYFVA
                     DWHAITSEYSSTEGIQDNVINMVIDWLSVGLDPQKSTLFLQSAVKEHAELFLLLSMIT
                     PLAWLERNPTYKEMKTELANKDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHVE
                     LTREIARRFNFLYREIFPVPEPILTNVPKLLGTDGRKMSKSYDNSIYISDRGKNLQHK
                     ISSMFTDPQRMRKKDPGNPDICNVFTFHGLYSSPEKVKEIDVECRRAGIGCTDCKKML
                     AQRVAENLQPVHERMDYYVSHMDEINGVIEEGNRKAAKIARQTMSEVRAAMKI"
     gene            complement(11056..11328)
                     /locus_tag="PLR13_05005"
     CDS             complement(11056..11328)
                     /locus_tag="PLR13_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQC18726.1"
                     /translation="MTKAELIGVIAKSAGITKDKAGKALDTFQDAVTKELKKSGKLAL
                     VGFGTFSVVKRKAREGRNPQTGKTIKIPAKKVVKFKAGKELAGKVK"
     gene            11739..12659
                     /locus_tag="PLR13_05010"
     CDS             11739..12659
                     /locus_tag="PLR13_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQC18727.1"
                     /translation="MQSVHFEKIKSWFSLSRPAFHTVGILPFILGTILAYKINLVFDP
                     AVFALGVSAVILIMLSTYHMGEYFDYREDEISQRLYKSRFAGGSGVVPAKTVKRSVAL
                     RTGLISFIIAGIIGLTLQFILKTGPYTLLLGCLGAFPGFFYSTQPVRLVQKGVGEIFI
                     GFCYGWLPVASAYYIQTGFVHSVIHWITVPIGLTIFNVIFLNEYPDYEADKATGKKNL
                     LYRLGKRKGKVLFFVFSLLASTAVFSSPVFGVPFKAVYLYLPVAIVSLFIAVMMLRDQ
                     YENRKTMELLCGLNIAVNLGTSLVYILAYI"
     gene            12665..13891
                     /locus_tag="PLR13_05015"
     CDS             12665..13891
                     /locus_tag="PLR13_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18728.1"
                     /translation="MTNVKKKVKAFYNPPSLLLKQLGSSLLMAYLNNWRNYPRWGFQY
                     GFRSIPVANGALGMGCIGYPGHPVWEVTGACNLKCIHCHAVSDKAGKDELSTDEGKRL
                     IDSIASVSAFRTLVYTGGEPLVRPDIFELLRHSQQAGLANIIATNGTLIDEEMAFKLR
                     DHGVVCHAISLDAATPAIHDMVRNKPGAFDRTLRAIKATKKAGILLQINTTAMEYNMP
                     HLPELIDFADRCGAGIMLMYQLVAVGRGEKIEKATLKKSANKHLSEMIAEKQKNATTI
                     IEPVAGPQYWPYLLEKKGFTEGLRLKLAGKVFHGCAAGRGFVYVKANGDVWPCPFVEV
                     NAGNIRDRNFKAIYEESPVFQNLRRREELLKGACGECHYKTVCGGCRGRAHAYSGDYL
                     QEDPRCYIRETTQNHS"
     gene            complement(14007..15164)
                     /locus_tag="PLR13_05020"
     CDS             complement(14007..15164)
                     /locus_tag="PLR13_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HQC18729.1"
                     /translation="MRYSLKILLLILMAFFLGGCMRAVSQPSFKTLDSLIPVDATQVA
                     FSDDLEPASLETAIDRSIRFYEGRGRNNVYQIEDRQIDAQQFKETLLAFRALLGDAKD
                     PETLGKRMAEAFDVYQVTGTDDESRVLFTGYYEPLLEGSLQKTEKYKYPLYRVPPDLV
                     RKGTRIGRMQNDKFVPYYSRREIDVDGVLRGKNLELLWVSDPVDLFSLHIQGSGKIKL
                     ENGDLLTVGFANTNGRPFRSITKFLLDKGTIQHHEVTYRHDFLRGKRDEEIYEALSHN
                     ERYTFFRFLEKDPVGSLGETVTPDRTIATDPEYFPEGALAFIRLRKPVFNTDGHVKER
                     VEFSRFVLSQDKGAAIKGPGRVDLFCGFGEKAEKTAGTLKERGELYLLLKK"
     gene            complement(15161..15619)
                     /locus_tag="PLR13_05025"
     CDS             complement(15161..15619)
                     /locus_tag="PLR13_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQC18730.1"
                     /translation="MLRKARIDDVKIIHRLINLSSGKGEMLPRSLMDIYGSLRDFFVY
                     YDEDESRIIGICAMNIIWANLAEIRSLYVEESHRGKGVGRVLVEACISEAITLGLFKV
                     FTLTYKKDFFLKLGFKEIDRNLLPEKIWSDCFRCSKYPDYCDEVAMIVEL"
     gene            complement(15613..17325)
                     /gene="recN"
                     /locus_tag="PLR13_05030"
     CDS             complement(15613..17325)
                     /gene="recN"
                     /locus_tag="PLR13_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQC18731.1"
                     /translation="MLNELSIKNFAIIDELHVTFGEGLNVLSGETGAGKSIIIGAVSL
                     LLGDRATSEMIRTQADTATVEAVFDISADKPLRDKIDAMGFPVSDELIIRRVISHTGK
                     NKVFINGQAATLANLAAVSERLINICGQHEHQIILAAENHIDILDEFGGCLSERAVYT
                     EHFNQYQQIRSGIEKLEELSKSADARTELYEFQLKEIQDINPQPNEDTVLSDEKKVLV
                     NIQRLSEWAGNAFDLLYGDKNSVIVMLKEVQRQIKEIKKIDAGLKISEAEMENNFVAL
                     QEAALLLRDYGKGLFFDAERLAAIEERLDLIHRLKRKYGGSLEAVFQKKQDMEEALRK
                     VSSLDEELQKMIREEEAVKSRLRQSARNLSESRKKAAKTLEDKVGKEIRALNMPHASF
                     FVHFKNRADDNTDFFGPRGADDIEFDLSANKGEDARPLNKVASGGELSRIILALKNVL
                     SGTGSVLSMVFDEVDNGIGGAVAEIVGRKLRAVSANHQVICITHLPQIACFGDKHMFV
                     SKKVVKGRTLTFVNELDSEQKIEEISRMLGGVDVTPTTREHAREMLQNAGSHNLKVSG
                     GDTC"
     gene            complement(17318..18208)
                     /locus_tag="PLR13_05035"
     CDS             complement(17318..18208)
                     /locus_tag="PLR13_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQC18732.1"
                     /translation="MKIKKIGIAANIEKEKIADHVRSLKKWLEERGAKVFLETEISGK
                     ILSDGGYSWSELARKAEVAVVLGGDGTILRSARYLAVHRVPILGINMGGLGYLTEVNL
                     NEIHSTMELILKGKFVTEKRMMLDVSVQHGKTVAGIGSVLNDVVMNRGDLSRMNELEV
                     EVDGEYLTTYKGDGLIVSTPTGSTAYSLSAGGPIVFPGKDLILINPICPHTLTNRPII
                     FSEDAHLRITLWSKDKGAMLSLDGQETHKIKSGDVVIVKKSRHATMLILSPYRSYGEI
                     LRSKLGWGDLPSGAHKRKNA"
     gene            18385..19464
                     /gene="rdgC"
                     /locus_tag="PLR13_05040"
     CDS             18385..19464
                     /gene="rdgC"
                     /locus_tag="PLR13_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HQC18733.1"
                     /translation="MGLLKGGFTFSRFSVDGQLPQAFLNFVNNRIRANSFKNSFKSTE
                     EKTMGWVSLTDILDTDFKNANYSLGAYLIFSLRIDRKLIPPKLLKIKLMEEERRFLAE
                     SGKNKIFRQQSTALKDKVKLDLLAKLDAIPFFYDVCWAVEQNTLYFSSLSDKVIDDFA
                     DLFKKTFALNLKRFSPQENHLIRKDADPAESTSLIGREFLTWLWFKSEERNGRIVLPN
                     KEECEIHFLKRVSLEAGEGEYSQGVVCHGIHAELKEGKEAIRQGKKVKEAGIKLLHNN
                     NEWDFMFKADSFDFQSLKMPQTDWQDTPEDPSGSLLERIYLIENAVKTIDTIYESFLH
                     IRQSPQWKEKELKLLHKWLEKNPKD"
     gene            complement(19469..20521)
                     /locus_tag="PLR13_05045"
     CDS             complement(19469..20521)
                     /locus_tag="PLR13_05045"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HQC18734.1"
                     /translation="MIKVLVVDDSALVRKVLTEELSRHKDIEVVGGAIDPYVARDKIV
                     RLKPDVITLDIEMPRMDGLSFLSKLMKFYPMPVVVVSSLTPRDSQNAIRALELGAVEV
                     ICKPGSSLSTADLSVRLVEAIRAAARAKIAKPAVELVERESVPGYKIQLKTTHKVIAI
                     GASTGGTIAIEKILSRLPMNIPGIVVVQHMPAYFTASFAERLNALCKIEVREAKDGDT
                     AAPGLALIAPGGYHMLLERSGAFYQVRIKGGPMVHHQKPSVDVLFHSVAKHAGANAMG
                     VLLTGMGADGADGLLAMRQNKAYTIAQDEATSVVYGMPGEAVKLGAACDELPLDEIPG
                     RIITRLSGEDKKREDK"
     gene            complement(20518..20997)
                     /locus_tag="PLR13_05050"
     CDS             complement(20518..20997)
                     /locus_tag="PLR13_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HQC18735.1"
                     /translation="MKTKIEKILEEATIKTFEDICFMYLEPELKDSQAALEPDAAAEV
                     EFHGAYNGRLVIASRGGLFSAIASNILSSDHPSLQEKKDALGEIANIICGNIVPSLGR
                     RKAGGYKIQSPRHLGRDELLKEEKSKPSAVVILNFNQGRADIKLFVDDYISDQEIND"
     gene            complement(20981..21373)
                     /locus_tag="PLR13_05055"
     CDS             complement(20981..21373)
                     /locus_tag="PLR13_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQC18736.1"
                     /translation="MALNTLVVDDSSVMRTIIIKTLKLAGLPLGDIWEAKNGEEGLHV
                     LQDHWVDLAMIDIHMPVMDGEEMLIKVRENDQYKNLPVIVISSESDPGKIEKMLELGA
                     TFIHKPFTPEVLKEVIVEVTGVSHENED"
     gene            complement(21429..21911)
                     /locus_tag="PLR13_05060"
     CDS             complement(21429..21911)
                     /locus_tag="PLR13_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HQC18737.1"
                     /translation="MVYTVGVADLKISDESADMLITYALGSCLGVTVYDFRKKTGGLL
                     HCMMPDSGIDQNKAAGNPYLYVDSGMKAMMDKFYQKGSRKSDLIIRVAGGSSSKLNEN
                     EDFFQIGRRNFVSLRKYLWNEGLMLKAYDVGGYGSRTVTLELESGKMLIKSQTFLKQL
                     "
     gene            complement(21913..22812)
                     /locus_tag="PLR13_05065"
     CDS             complement(21913..22812)
                     /locus_tag="PLR13_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQC18738.1"
                     /translation="MHSEWNNLSTDYYSLKLTSEQFDKISRLVYQISGIDLHEGKEEL
                     VKARLIKRLRHLKFHDFNQYLKHIANDKSGTEIGAMVDILTTNKTNFFRESEHLDFLK
                     HEIIPGWVKGPVRIWSAGCSSGEEPYTIAIVLLESIPDTATLDIKILATDISDRMMEK
                     ARQGLYDEESLAIMSRHLKHKYFQKTDTGSGYKYKVIPKIQSMVSFAKLNLMEHWPMK
                     GPFDVIFCRNVMIYFDKSTQEKLVGRFWHLLKEDGYLMVGHSESLSFLQHEYRYMRPA
                     VYQKIRKAHSVVKKTGKVRTDVK"
     gene            complement(22881..24983)
                     /locus_tag="PLR13_05070"
     CDS             complement(22881..24983)
                     /locus_tag="PLR13_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQC18739.1"
                     /translation="MINNMKMGVKLAGVFAIIFVVVTAMMLSGAAVRMKALRMLDSNY
                     RDYVNDIKNIGTIEVGLVNMEKDLHLYVNSPAARENALIQIQEGITSTENSFQSLKSS
                     KRDSEEQKYLSEFEVAWPEIQRGYKAVIKAVDEGKNDDANSILADKSYLNEAGRKALA
                     VVKNLNQYSVNKNETRVKQYVKGRGGWSGILWIFSGFGALALIGLGTMVHRNITRPIK
                     TIVLMMQEIEKGHLSKRLKMNRKDEIGSLSRSMDMFADNLQKNIVYSMQQISEGNIDV
                     EPKVTDSKDEIGPALIKMINAIGSMSNEVKRCCLNALEGNLQNRADVTPYQGSFRQII
                     QAFNDTLDAVMNPINEAALVLTKVADKDMTARMAGDYKGGHAKIKESVNMVVDNLDGA
                     LQQVAISADQVASASVQVSTGGQSLSQGASEQASSLEEVSSSLQEMSSMTKQNTLNAR
                     EAKGVAEQARGSAERGVESMSRMSSAINQIKSSSDATSKIVKTIDEIAFQTNLLALNA
                     AVEAARAGDAGRGFAVVAEEVRNLAMRSAEAAKNTASLIEEAVRNSENGVTINTEVLT
                     NFQEITEKINKVSQVVAEIAAASEQQDQGINQVNKAVEQMNQLTQQNAANAEESASAA
                     EEMSSQSEEMRAMVAGFRLTKTVALTAGPDAIKKTEYRLPDVAPGKKGDKASAGLHPD
                     PRKVIPLDDKDHDILQNF"
     gene            complement(25094..27079)
                     /locus_tag="PLR13_05075"
     CDS             complement(25094..27079)
                     /locus_tag="PLR13_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQC18740.1"
                     /translation="MLNDIKVGVKLIGSIVTVCLIAAAVILYGIKVRNDVVHKVDSVH
                     RQYEINIRDIGNVRVSLEKMGGDIYRYFGAPADRQKMSKEINETISSVDGTMQSYKNR
                     SIDVDEKKLIADFESAWSEMQRRYKEGMKAVDEGRQNDINQFIGAGSAAMQAREKTSA
                     TLRDLNNYSVNKNESAVKATIDNVGGLSSIMLILSAIAIALAIVTIVFLTESITKPLK
                     KGVQMMAEMKKGHLSNRLDIKRRDEIGLLANAMDEFADHLQKNIIFNMQQISEGNINV
                     APVVIDEKDEIGPALKKIIETINSLLNEVDMLTKSATEGKLDVRGNEQDYKGAYQGIV
                     QGVNRTLDAVIDPINEASAVLERIADKDMAARMVGDYKGGHAKIKKSLNLAVENLDRA
                     LQQVAIGADQVASASVQVSTGGQSLSQGASEQASSLEEVSSSLQEMSSMTKQNTLNAR
                     EAKGVAEQARGSAERGVESMSRMSSAINQIKSSSDATSKIVKTIDEIAFQTNLLALNA
                     AVEAARAGDAGRGFAVVAEEVRNLAMRSAEAAKNTASLIEEAVRNSENGVTINTEVLT
                     NFQEITEKINKVSQVVAEIAAASEQQDQGINQVNKAVEQMNQLTQQNAANAEESASAA
                     EEMSSQSEEMRAMVAGFKLTGSGYTERESIAYEPKDF"
     gene            complement(27134..27667)
                     /locus_tag="PLR13_05080"
     CDS             complement(27134..27667)
                     /locus_tag="PLR13_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQC18741.1"
                     /translation="MNNANAVKNDQSANADSRAGKYLTFFLASEEYGVEILKVQEIIG
                     RMPITPVPLTSRYIRGVINLRGKIHPIMDLKIKFGMEETQITDETCIIVIKTTSMMMG
                     ILVDKVSEVINIASGDIEDTPSFGGDVNPEYLLGVGKTGGRVRLLLDIEKVITASDII
                     NMKKAAAGEGKDAMAEL"
     gene            complement(27695..29764)
                     /locus_tag="PLR13_05085"
     CDS             complement(27695..29764)
                     /locus_tag="PLR13_05085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQC18742.1"
                     /translation="MNLNRLAISLIELNPENPADLEEFKKNLLSCYNNTQGRIQNILK
                     EACFEADAMVDQVPELQRNIIARLGVLLEQAMGSDETDISATTEQTSQVEPAREPDSI
                     DVQHVMLSADLDADLLNEFIIENMEWATMAESAILDWEQNPGNQELLNTIFRGFHTIK
                     GTSAFVNLDCVKDLAHQVESVLAKVRDGEHVFTRSYANLALKSLDVIKSILEQMKSSG
                     PGQQIELPRGYQGLLESLRNFVPDEQVQEMAIPAEESMVEPEAAAPAYTKTNRGKIAK
                     KEGEASSEQSASSNKIREQMTESTVRVKVDRLDKLLDTVGELVIAQTMVGQDNLIVSG
                     KYHELNKKISQAGKIVRELHDLSMFLRMVPLKGTFQKMVRLTRDLSLKHGKMVSCITE
                     GDDIEIDRNMVDMVADPLIHLIRNAVDHGIEFPEERRRAGKSENGRIYLTAGHAEGSI
                     MLTVWDDGRGMNRQTIVNKAIQKGLIDSAERMTDQEVWQMIFLPGFSTAEKVTEVSGR
                     GVGMDVVKQAVEALRGRIEVQTTEGQGCTFIMRMPLTMAITDGMIVKVGSQRYILPTA
                     NIHKAVRPQESDIHTVNQRAEMLSFRDEMVPVIRLYRFFDIPDSLTDLTSGLLVVIGE
                     GKQRCALFVDELLGQTQVVTKLLGKGMNNVPGIAGGAIMGDGTVGLILDASGIVSIAR
                     QQTNTTT"
     gene            30182..30562
                     /locus_tag="PLR13_05090"
     CDS             30182..30562
                     /locus_tag="PLR13_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQC18743.1"
                     /translation="MIKKILIVDDSPISVKIIKSCVPKDMGYELYDAADGQMGVEKYK
                     EIKPDLTFMDLTMPVMTGFQALEEIMKMDTKAMVIILTADVQIKAIAKAHDLGAFSVL
                     KKPPSKESVAGAIKEAEEALASRG"
     gene            30566..31216
                     /locus_tag="PLR13_05095"
     CDS             30566..31216
                     /locus_tag="PLR13_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HQC18744.1"
                     /translation="MSQMDNIQGLITSAETDLLQEIMNIAFGRAASDLAEFIDIFVVL
                     SVPQIKLLPAYQLPDYINSEIMDYDQDRVSVVEQSFWGKFKGNALLVFPSGTGKRMIS
                     LFDGKDEFFQSDPSLEQEKETFLEIGNILIGACIGKLAELLGDIATYSPPRVLVEKSL
                     HRVYESIINPDSMAIVLRTVFSFNEKNISGFMFVLTKQESFAWLKNALHKFLEQYE"
     gene            31982..>33557
                     /locus_tag="PLR13_05100"
     CDS             31982..>33557
                     /locus_tag="PLR13_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18745.1"
                     /translation="MTHLKLVEAMQKPDFYPHRPANVEFIQTHISYVFIAGDYVYKVK
                     KPLKFDFLDFTTREKRKFFCEEELKLNRRLAPDTYLDVVSLSRNEAGDITLDQGIEIV
                     DYAVRMKKLPGDRMLKTLLARGTADEKIMDALARKIVLFHQQARTDSRIDKMGDIENI
                     RHNHEENFAETEKFIDVTISEYQYSLIKDFANKFLIENKKHFEQRITDHKIRDCHGDL
                     HLDHICINDEITIFDCIEFNERFRWGDVAEDVAFLTMDIDFNGWPHHAENFIQSYLKY
                     SGDTGLLKLLNFYRCYYAYVRGKVTSLRLLQEDTTADEQRDITETAEHYFDLAYTYAA
                     RLDKPVLILTAGLMGSGKSYQARRLAVRLGTEIIRTDELRKALFNIRTTERRHERFGD
                     GIYSDDISRLVYEKALALAEQKINLGKAVIIDASFKKRTERLKALALAKKLQIRFFVL
                     ECFCPDDITQKRLEKRVLDNDNVSDGRWELFQQQKDNFEPIDEITADCRFKIDTSEKP
                     EVFRHEMIRKIKLAEFE"
BASE COUNT         8327 a         8517 c         7262 g         9451 t
ORIGIN      
        1 agcaccttca ttgagccgct ggcctgcgtt gtcagagcgc aacggctggc ccgcgtaaaa
       61 aaaggacaga cggttctggt aatcggctgc ggcatgtccg gcctgctcaa cgtaaaactg
      121 gccgtcacca aaggctgccg gatcatcgcc tccgacatta accgcaagaa actcgatatc
      181 gccctggaaa tgggagccca tattgctatc aacgcagccg atgatatttc aaaacggttg
      241 gtagatcaga ccggcagaaa ggccgatatt gtttttctgt gtgcttccgc cgatgcggcg
      301 gtggaacagg catggcaatg cgttgacaaa ggcggcgtca tcgttttgtt cgccgtcccc
      361 gcaccggata aaaaagtggt tgtaccggtc aacgacttct ggatgaagga aattgccatc
      421 ctgacgtcgt attactgcgg tccttctgac attattgaag caatgaaact gatagagtgg
      481 catcatgtcg ccatcgatga tctgatcacg caccggttgc cgctgacgga gatcgtaaag
      541 ggctttcaac tggttgccga cggcagggaa tccatcaaag tcatcattaa accgcaggag
      601 caggcggccc tttaatgtcc attaacggac catgacatcc ttaatggtct gcgctacctt
      661 ttggtcctca aaaagctttt caatggtatc ggacagaacc ttgtccgcct gctgtccgag
      721 aatattttcg gaaaacaaaa tatgttcccg gctgtaagaa ctcttcaccc gcgttttgta
      781 cagtatcctg ccttggccca tatccgcgat aacgacggcc agcgtcatcg tcgtatcgta
      841 ggaattcgtc agttttcctt tccggcagat gatttccagt tcttctatat taccaccgat
      901 cagaattttg gccttgccca tcggcatttt atcttctttc aaattccacg gttcattgat
      961 ttcggtaatt ttatacccgg cttttttcaa atattgcttt atcccgttcg caacagccct
     1021 cgttgctctg acattgcggg ggaacaccag ggttttttcg ccgctgtttt gaatcacgcg
     1081 gccgatgacc agcgggtcgt caatctgacg tgtatcggca aattcggaaa cggccacaac
     1141 agcgtcggat gtctgcccgt catttttcac atacgcgggg atcaccgatt gctctgcgtc
     1201 ataatacata ccgacactgt agagtctggg tccggcgcag ccggatagac aaaaaacaga
     1261 aaaaatcatt acgacggcga aaagacgata tatcggctta agattcataa acactccctt
     1321 cattatgatc aatcaggatc ataaaccctg acttttttct ttttattttt gaaagatata
     1381 taatataatt ataaaagttt agcaaagtaa aagatgggaa gcattgacaa ccttaaccga
     1441 ttttttaact caaaaaattt aaggagggca ttatgacaga agcaacccag caagctttaa
     1501 ccggcctgag agatctttcc atgatcaaat ggtatgtaat tcctcttttg gctatcgtct
     1561 tttacattta caccaaggaa atcaaagatg cacgacaaaa cggagactgg aatgcggttc
     1621 tcgccggcct gactattttc ggcgtggatt tcttcaacga aacatggaac ggctgggtca
     1681 tgtacctgac gcagcgctcc gctttctgga caacccccgg cgataccgcc ttgcggacac
     1741 tggtcggatg gaacatcgaa attatgttca tgttcttaat catcggaatt attttctatc
     1801 atacgttatc cgaaagcaaa aaagataaaa tcctggggct gcctgaaaaa tggttctggg
     1861 caatcagcta cagcatcttt tgcgtttttg tcgaatgcct gctcaacatc ggaggccatc
     1921 tggtctggga atatcctttc tggtatctgt cctttaaagg cgtctggctc atttttctga
     1981 tcggctattt ccactttttc tgctttgcca ttctggtcat cagcctgaaa tccataaagg
     2041 ccaaactgac gaccgttggc atcatctacg ccgcgccgat tattatgaac gtactggcat
     2101 ttggattctg ggggtggaat tattagccgc taaagcccgg aatagtcgtc ataaatttta
     2161 ttatattttt cgtgaatcat tttgcggtcg aattcccaga attcgtcaag catcttggtt
     2221 tgctcttcct tgttgaagta ttcctgacag ggataaaaga agtgtttgtc ttccttttcg
     2281 atatgcttgg gataaaaatt gcccagttgt cttgccgtat caatcaggcg gaataattcg
     2341 gcctgattgc cctgcaaata tttatttttt gcctccacca gcgcagccgt tgtctttctg
     2401 ccccagacat gttcgtcaag cagttcctgc atgatgttct tcaaatcggc aggaagatct
     2461 ttttttgcca gatcccggaa aagaatctct tcttccttgc cgtgatgcgt tcggtcggca
     2521 tagacacgga taaaatcaac ggccatatca ataaccagcg gattcacctt ccgggtcttt
     2581 tccacctgat ccatatggcg catcattgca gtcagcattt tttcgattaa ccggtgttcc
     2641 cacatcaaag gtccgatggg tttcataggt ctgcctcctt tttttgaaca tgatatgttt
     2701 ctatatacca tagaggagga tttttttcat tgccttctta ttcatggggc aatatatgtt
     2761 gcatcaccat tgcatttctg ataattttca aaaaccaggc tggatacgaa agaatttgac
     2821 gtcccggcaa aaaaccgcat ataaaaacgt cagatttcat tcgtcgcgtg tttgatgaaa
     2881 ggacacgaca taatttattt gctatgatca accctgccct gagaaagaag attgatgatg
     2941 cttttaaaaa gaaccccgct gttgatcctg ctttgtttgg ttttaatctt ttcctgccag
     3001 caggccaaag accccggccg gaatgacaag aaactcaatg ttattgccac aatttttccc
     3061 gtttacgatt ttgcgcgcca tatcggcggt gataaaatca ccctcgccat gctcttgcct
     3121 ccgggaacag acgcgcatca ttacgaactg aagccggatg atatcatcaa agtcaccaac
     3181 gcggatgttt ttctctttac gaattttgaa ttggagcagt gggcctataa aatcatcggc
     3241 gcctccgata aaaacacccg tctgcaggcg gtggaaaccg ggaacggcgc catgatgctg
     3301 aggctgaaca aggaagaaga cgatcatgag gcacatcctg ccggttttga tccgcatatc
     3361 tggctggata tggataacgc tcaaaaaatg gtggacaata taacgagtgc gtttattaaa
     3421 aaagatcctc gcaacagtga ctattacttg aaaaatgccg gtgactacaa acgccagttg
     3481 gcggacatgg atcaaaaata ccggacaaca ctggccgcct gtaaaacgag aacggttctg
     3541 catgcgggtc actgggcatt tgcctatctg acgtcacgat atcatctgaa atacattgcg
     3601 gcctataatg ttttcgctga tgcagaaccg gcaccgcaga aaatctttgc cttaatcgat
     3661 cagatcaaaa atgaaaacgt atcccacgtc tattatgaag acatgatgaa tccgcgtctg
     3721 gcaaaaacca ttgcccgaga aacgggcacc ggtctgctga aactcaataa cggacacgat
     3781 gtcaccagaa cggatctgga aaaaggagaa acctttcttg ctttaatgga aaaaaatctc
     3841 gtgaattttc aaaaaggatt acaatgtccg gagaaataat caaggtcgat catctctcgt
     3901 tctgttacaa cgggacaaaa gtaatcaacg atatttcctt taccgtccag agaggagact
     3961 atctgggcat cgccggttcc aacggatccg gcaaaagcac tctgatcaaa aatatactgg
     4021 gcatccttga acctcaccag ggacatataa caatctttga tcagcccgtt gcatcctttg
     4081 atcagtggca cagaatcggt tacctgccgc aaagaatcaa cgcgctgcat taccatttcc
     4141 cctgcacggt cgcggaaatc gtacaactcg gcatcaaaaa aaggaatccg gatgatctta
     4201 aaagaaccct tgatctgatg ggtatcgcgc atctttcctc ctgcctcatt ggagaactat
     4261 cctacggtga acagcaacgg gtcatgctcg cccgggcttt gatcagaaaa ccggatttgc
     4321 tgatttttga tgaaccgaca accgctttgg atccggaaac gagagatgtt ttctacgccc
     4381 tgaccgcgaa ggccaatgag catggaacga cggttgttct tgttactcat gacacaggga
     4441 cgattggaaa atatgcgcgc aatcttctct acctcgacaa gaaaataatc tttttcggaa
     4501 ccttcaggga cttttgcgca tcgccggata tgacgggatt tttcggaccg aacagccagc
     4561 acatgatttg ccatcaacac gatcagaaag gacccgcaac gccatggaac tgagcggcat
     4621 ttttcagtat gcattcgttc agagagcctt tctgggaggc atactcattg cggcgatagc
     4681 ttccatcctg ggcgtatttc ttgttctgcg ccgcttttcc ctgatcggcg acggtctttc
     4741 acacgtcacg ttcggaagtg tcgccgtcgt catgctgctg ggtatcagtc ccgcgcatat
     4801 gacgctggcg gcgctcccgc tggtgatgct gtcgtcgctg gccattctga aactgacagg
     4861 ttcaaaacgg attcagggcg acgcggccat cggaatcatt tcttccttcg gtattgctgc
     4921 aggcattatc ctgaccagta tctccggcgg atacaacctt gatcttttca gctatctgtt
     4981 cggcaacatt ctgaccgtaa ccctaacgga aatctttttt gccatcatcg tttttctgac
     5041 cgtcgtggcg acagtgatca tcctgtacga tgatcttctg gccatcacat tcgatgaaga
     5101 actggcccga accatgggcg ttcaaaccgg aaaaattaat acggttctct ttctgctcac
     5161 agcggttgcg gcggttttag ccatgaaagt cgccggtatt atgctcgttt cggccatgtt
     5221 gattatcccc ccgctaaccg ccctgcaatt gtctttaagt ttcagaatga ccatttttac
     5281 gtcaattatt ttctcagtat tatcggtcat attgggaatt acgatggcct ttttgttgaa
     5341 tttaccggcc ggaggaacca ttgtggtctt aaacatattt tttcttttat gtgttttttt
     5401 cgccagaaaa ttaatggctt tcgggaggtg acgccgaatg acccttccct ttcaggcata
     5461 ccgttcaagc atcattttca ttatcgcgct gttttgcggt atggttttcg acgaggggcg
     5521 tcaggcggga agaatactta ttctgccagc cctgatgatt attctgacca ttacgttact
     5581 gagatttcca cgcggttttt tccgtcatcc cgcttctctt ttgttttcct ccatccgggg
     5641 caaccttttg aattatctcg ttttgggaaa tttcatcctt ctaaccggtg cctttctgat
     5701 tcaaaaacag gaactctgga tcggcctggc gctggtcgct gccatgcctg catcgctgga
     5761 aattattctt ctgggaatat tactgcgtat tgagaagaat tatgtcttta catcaatatc
     5821 gggaacctat ctgggtgccc tgctgatcgt gcctttagtc agcttatgct ttttcaaata
     5881 tatacagata aactactgga acattgccgt gcttatcctg tgtctgatcg ccctgccgct
     5941 ggttctttcc cggctgattg tagagaaaga ttgggaccgg attctcagga aacatgaaaa
     6001 tacgctgatc gacttctgtc attttatcgt tttttatgcc atttccgcaa acagcagcat
     6061 gtttttaaaa aagctttcag acgatcttgt gtttatatcc ctgatcgctt taaccagcac
     6121 ttttttattt tattttctca ttagaagaat cggtctctac tttcacggtc aggaaaacaa
     6181 ggtgaatttt ttcatcctgc tcggaaccat gaaagattac ggactggccg gcgggatagc
     6241 cctgaccctt tttaatccgg aagtcgccgt gccaccgctt gtttttgccg tatttacgtt
     6301 tatttacatg aactggctca gtttcaaggc aagacgcata gaagacgatt gcggcgatgt
     6361 gtgaaaaacc tcaaatatca atttgacaaa tttaaaacac gacctattat aaaatacgaa
     6421 cacgtgttca gtgtattccg atgctctttg taatcggaat aagtctgtta agtatttttc
     6481 aacttctctt gaatgcagca agaaggtaaa aatcaactct caaaaaagga gaataattat
     6541 ggccattaca ttgccggaat tgccctacgc aaaagatgcg ttggcgcccg tgatcagcgc
     6601 caataccctg gattttcatt atggtaaaca ccataaggct tacgtggaca acttaaacaa
     6661 gctcattgcg ggaacggatc ttgatcaaaa atcgctggaa gaaatcatca acattgccgc
     6721 taaagattcc gcaaaggccg gcattttcaa taatgccgct caggtctgga atcacacctt
     6781 ctactggaaa tgtctgaaga aaacgggggg cggccgtccc agcggcgcca tcgcggctaa
     6841 aattaatgaa atgtggggga attacgataa atttgttgaa gagctgaaaa atgcaggcgc
     6901 cacacagttc ggcagcggat gggtctggct tgtttcggac aaaacccggt tgcagataac
     6961 gaaaaccgcc aatgccgaca cgccccttgc gcatggtcag aaagctcttt tgaccatcga
     7021 tgtctgggaa catgcttact atctggatta ccagaatagg cggccggatt atctttccgc
     7081 cgtgattcag aatctgatta actgggattt cgttaatgcc aacctatagg cagggcgtct
     7141 tcaatcgtta aaacaataaa aggaggcttt aaaaatgcct ccttttttat aggataaaaa
     7201 cagacaggaa tgattatttc tttttatcga ctctcacttt aagctctttc ccgactttaa
     7261 agaagggcaa ttttttaggt tcgacctgaa ttttcgatcc ggttttcgga tttcttccgg
     7321 tatatgatcc gtattttttc acaacaaaac tgccaaatcc tctgatttca atacggccgt
     7381 ttttctttaa ctcatcggta aaacccttaa aaatgagatt cacgacatca attgcctttt
     7441 tttccgtcag atttaacttc gtgcttaaag cttcaataag atcggatttg ttcatatctc
     7501 ctccttaaac tgatggcaac tcatactagc cagatgacac tgtattttga tattctctat
     7561 gcgtgtgttt attatttatt ttttggcgaa agtcaaggaa attttgctat ttggacttga
     7621 ctgcactgtc cagcaattga ctgatttcct tccgggtcag atggcgccac tcgccctctt
     7681 tcaaggtacc caacgcaaga tcaccaatcg cctcgcgcac aagcagggcg actctgtaac
     7741 cgattttttc aaaccctctc cggataattc tgtttttccc ttctttgagc gtaagacgca
     7801 accagcagct cctgtcgttc attttatcca ctttgagatt ttccggtttg aaaaaatcat
     7861 cgtcaacgtt aatgcccctg ctcagttgct ggacatcttg tctggataaa tgtccctgga
     7921 ttttgacttt gtaaattttt ggtttctgaa aacgggggtg ctgaattttc tgggcaaaat
     7981 cgccgtcatt cgtcagaagc aacagacctt tggaatcgta atccaacctt cccaccggat
     8041 aaaccctctc cggcacactg cctagcaaat ccacaaccgt gggcctgttc tgaggatcat
     8101 gaagggttgt tacaaatccg gccggtttat ttaaaacaat ataatatttt actttttccg
     8161 tggagatgat cttgccgtca acgcgaataa tatctttctg agcatccgct ttttctccca
     8221 gtttcgttac gacaacaccg ttgacgctga cccgtccccg ggtgattaac tcttcagcgt
     8281 gacgccgcga ggtaatgccg gccgctgcaa taactttctg caagcgctct ttcataaatc
     8341 aggaacttct tctatctttt cttctgcatt ttcttctgca ttttcatcgt catgctgttt
     8401 tgcgacagcc tcttcttcta cgatggtttc ctgttcggac tcgctgcttt cctgatccgg
     8461 atcaagaagt tcctgctgct gatgaagttc ctttaactcg cgaatattgg gcaaatccat
     8521 aatgtctttg aggttaaaga cctccagaaa tttccgggtt gtcccgtaaa taatcggttt
     8581 tccgggcaca tctttacggc ccacaatacg aacaagtttc ttctccagca gacctttgag
     8641 cggacctccc acatccactc cgcgtatgtc ttcaatttcc gatttcacaa tcggttgctt
     8701 ataagcgatg atggccaggg tctctaacgc ctgcggggac agagactgcg gtttggcggt
     8761 tttgagcttc ttgatccaca ggctgagatc gggattggtg cggaattgaa aaccgcccgc
     8821 aacttcctgc aggagaattc ccccctgacg gtcatggtat tccgagacca gttgatcaat
     8881 gatttcccgt atctctgttt tttccacctc cggaaaaacg gcgcgaattc tttcaggtga
     8941 caacggggtg tcggaagcaa aaatcagagc ttcgatgacg gctttaatct tttccattta
     9001 cgactccaca gccggaaata tccggataac accaaacatt gaagtttgat aggctttaat
     9061 cattctgagt ttgatcaatt ccaggatggc taaaaatgta tagataatgc gacggcggtc
     9121 ttttttcccc ttaagcagat cttcaaacgt gatgtttttg gtcaccgtca actgttccat
     9181 gatttcgttg atgatatcgg tcagcgacag tttttccaga tcaatttcca tcaattcttt
     9241 cttatccatc tgagagataa tacgatggaa ggcctcgatc agttcaaaaa cgctgacctc
     9301 aataaactct tcctcttcat ccgttgattt gacctccact tcagggagag ctgccgcacg
     9361 ggtgaagaca tctctttcca gaagaggccg gtttcccaga ctgaccgcaa tctccttgaa
     9421 ggttttatgt tccaggagct gtctgaccag ttcggcacgc ggatcttcct cttcctcttc
     9481 gggttcttca ggcggcggta aaagcgttct ggatttgata tgaataagcg tggaggccat
     9541 caccagatat tctccggcca ggtccagatt cagcgctttg atcattttca ggtactggaa
     9601 atactgctcg gtaatcagcg caatggggat gttgtaaatg tcaatttcat tttttttgat
     9661 cagataaagc aacagatcca gcggaccttc aaagatatcc agcttgatgg cataattcaa
     9721 acgctgctca ttgtccatag gatccatggg atgacctaaa ttattattat ccatgacgga
     9781 aattcaaatc ttcattgcgg cccggacttc actcatcgtc tgacgggcga tttttgccgc
     9841 ttttcgattg ccttcttcaa taactccgtt gatttcatcc atgtgactta cataataatc
     9901 catgcgctcg tgaacgggtt gcagattttc ggcaacgcgc tgggccagca ttttcttgca
     9961 gtccgtacac cctatccccg ctctgcggca ttcgacatcg atctctttca ccttctccgg
    10021 tgaagaatat aatccatgga aagtaaaaac attgcatatg tcaggattac ccggatcttt
    10081 ttttctcatg cgctgaggat cggtaaacat ggagctgatt ttatgctgca gatttttacc
    10141 gcgatcgctg atgtaaatgg agttgtcgta ggatttgctc atctttctgc cgtcggtccc
    10201 cagcagcttc ggaacattcg ttaaaatcgg ttccgggaca ggaaatattt cccggtaaag
    10261 gaaattaaac cggcgtgcta tttcccgggt caattcgaca tggggaagtt gatcaacgcc
    10321 taccggaacg ccataggctt tgtacatgat aatgtcggca gcctgcagca ccggataacc
    10381 caggaaaccg aacgtcgata aatccttatt ggccagctcg gttttcattt ccttgtaggt
    10441 gggattgcgt tccagccagg ccagcggcgt aatcatagaa agcagcagaa aaagctccgc
    10501 gtgttctttg accgcggact gcagaaacag agtgctcttt tgaggatcaa ggccgacact
    10561 gagccagtcg atgaccatgt tgatgacgtt gtcttgaatg ccttccgtgg agctgtattc
    10621 gctggtgatc gcatgccagt cggccacgaa ataaaaacag tcgtattggc cgctttcctg
    10681 caatgcaatc caattgcgca atgcgccgtg aaggtttccc agatgcagct tcccggtggg
    10741 cctcatcccg ctgagtatcc tcttacgctc caaaacaact ccttttgcat atgttactcg
    10801 gcatcacagg ccgtcgattc ctgtttcgcc gtactgtccc gccgagcgtc gctcgcggga
    10861 tagttttcca cggcttatgg ctgtggaatc attcgaccag caagcttccg ttaacgacgt
    10921 ttgtgaaggt tgggtctcat ggcaactgac aaatttcaat gcttcgtttt taaaaattcg
    10981 ggtctcacta acgaactaag gcctccggct atgacagaat acggaggcct tagttttaaa
    11041 gaaccaattg atttgttatt taactttacc ggccaattct ttgcctgcct tgaacttaac
    11101 gactttcttc gcgggaatct tgatcgtttt gccggtctga ggattcctgc cctctctggc
    11161 ttttctttta accaccgaga acgtgccaaa gccgaccaga gccagtttgc cgcttttctt
    11221 gagctctttc gttaccgcat cctggaaagt atccagagcc ttgcctgctt tatccttggt
    11281 aatgcctgct gatttcgcta taaccccgat taattctgcc ttcgtcatct tctaaaatcc
    11341 ccctttcgta aaatcaaatt ttgaatttta ttatcaaccg ccttcgcggc cataaccaaa
    11401 accatttgaa acagataaat cgctctggca aacatcacga aaaatgcttc gttattcttt
    11461 attttgcaaa ctaacgttac tcaaaccggc ttgttctctg gtttagaaag gagccatccc
    11521 cctcaagctc gcgctacgac tagcacaata aagattaaca atcaagaaaa aaatgttttt
    11581 ttaatgcttt ttcccgtccg tgtgtgctgc ggcgatggca tcctgatcgt cgtgctgcct
    11641 gcaccgccag tgtcccatgg aagtcggcaa tcggctgcca tgctttacaa aaggtacctg
    11701 ttatgttaag acctgcacct tattttttgc gggggtctat gcagagcgtc catttcgaaa
    11761 aaataaagtc ctggttttca ctctcccgtc cggcgtttca caccgtcggt atcctcccgt
    11821 ttattctggg aacgattctg gcatataaga tcaaccttgt cttcgacccg gctgtgtttg
    11881 cgctgggtgt ttcggcggtt atcctgatca tgctttctac ctatcacatg ggagaatact
    11941 ttgattaccg ggaagatgaa atatctcagc ggttgtataa aagcaggttt gcaggcggat
    12001 caggcgtcgt tccggcaaaa acagtgaaac gttcggtggc tttacggacc ggattgatct
    12061 cctttattat cgccgggatc atcggactga ctcttcaatt tatcctgaag acaggcccct
    12121 ataccctgct tttaggctgt ctgggcgctt ttccagggtt cttctattcc acccaaccgg
    12181 tcaggctggt gcagaaaggc gttggggaaa tttttatcgg tttctgttac ggatggcttc
    12241 cggttgcctc tgcctattat atacaaaccg gcttcgttca ttcggtcatt cactggatca
    12301 ccgtccccat cggcctgacg atcttcaacg tgatcttcct caacgagtac ccggattacg
    12361 aagcggacaa ggccaccggc aagaaaaacc ttctttatcg cctggggaaa cgcaagggga
    12421 aagtactctt cttcgttttt tctctcctgg catcgacggc cgtgttttcc tctcccgttt
    12481 tcggagtgcc gttcaaagcg gtttatctct atctgcctgt agcgattgta tctttattta
    12541 ttgccgtaat gatgttacgt gatcaatacg aaaacagaaa aaccatggag ctattgtgcg
    12601 gtctgaatat agcggttaac ctgggaactt ctctggttta tattctggca tatatttaag
    12661 cgaaatgacg aacgttaaaa agaaggtcaa agcattttat aatcctccct cgttactgct
    12721 caagcaactg ggcagttctc tgttgatggc ttatctcaat aattggagaa actatcctcg
    12781 ctggggcttt caatacggtt tccggtccat cccggtggcc aacggcgcct tggggatggg
    12841 ctgcataggt tatccgggac atcccgtatg ggaagtcacc ggcgcgtgta atctgaaatg
    12901 tattcattgt catgcggtaa gcgataaggc gggcaaagat gaattgtcca ccgacgaagg
    12961 caaaagactg attgacagca ttgcctctgt aagtgcattc cgcaccctgg tttatacagg
    13021 cggcgaaccg ctggtgagac ccgatatttt cgagctgctc cggcactcgc agcaggccgg
    13081 tctcgccaac atcatcgcca cgaacggcac gctgatcgac gaagaaatgg cttttaaact
    13141 cagagatcac ggtgtggtct gtcatgcgat cagcctggat gctgctaccc ctgccattca
    13201 cgacatggtc cgcaataaac ccggtgcctt cgaccggacg cttcgcgcta ttaaagccac
    13261 aaagaaggcc ggcatccttt tgcagatcaa cacgacggct atggaataca atatgccgca
    13321 ccttcccgaa ctgatcgatt ttgcagaccg ttgcggtgcc ggcatcatgc tgatgtacca
    13381 actggtcgcc gtgggccggg gtgaaaaaat cgaaaaagcc acgttaaaga aaagtgccaa
    13441 caaacattta agtgaaatga tcgctgaaaa acagaaaaac gcaacaacca ttattgaacc
    13501 ggttgccggt cctcagtatt ggccgtacct gctggaaaaa aagggattca ccgaaggact
    13561 ccggctgaaa cttgccggta aagtgtttca cggttgcgca gccggacgcg gttttgtgta
    13621 cgtcaaagcc aacggcgatg tctggccctg tcctttcgta gaggttaacg ccggcaatat
    13681 ccgtgacaga aattttaagg ccatttacga agagtcgccc gtatttcaga atttgcggcg
    13741 ccgggaagag cttttaaaag gcgcctgcgg cgaatgccat tacaaaaccg tctgcggcgg
    13801 ctgccgagga cgggcgcatg cctacagcgg agattatctg caagaagatc cccgatgtta
    13861 tatccgggaa acaacccaaa atcattcgta gcttttctca aggatcgcca tgaatccgga
    13921 cgaaacaaga agcttctgcc tttgcgtcgt gcaagcccct ccctgacgtg gaacagctct
    13981 agcggccatg cgctggaaaa acggatttat ttcttcagca gcaagtagag ttcgcctctt
    14041 tccttcagcg tgccggccgt cttttccgct ttttcaccga atccgcaaaa caaatccacc
    14101 ctaccgggtc ccttgatggc ggcgcctttg tcctgactga gcacaaagcg ggaaaattcg
    14161 accctctcct tgacatgtcc atccgtattg aaaacaggct tgcgcaaacg gatgaaggcc
    14221 agggctcctt ccgggaaata ttccggatcg gtcgcaatcg ttcggtccgg tgttaccgtt
    14281 tcgcccagag atccaacggg atctttttcc agaaagcgaa aaaatgtgta ccgttcgtta
    14341 tggcttaaag cctcataaat ttcctcgtca cgtttccccc gtaaaaaatc atgacggtac
    14401 gtgacttcat gatgctgtat cgtgcctttg tccagcagga acttcgtaat gctgcggaaa
    14461 ggccggccgt tggtattggc aaaaccgacc gtcagaaggt ccccgttttc cagtttgatt
    14521 ttgccggacc cctggatgtg cagagaaaat aaatccaccg gatccgaaac ccagaggagt
    14581 tcaaggtttt tgccccggag aacaccgtcg acatcaattt cccggcggct gtaataggga
    14641 acaaatttgt cgttctgcat ccgtccgatc cgggttccct ttctcaccag atcaggcggt
    14701 acccgataca gcggatattt gtatttctct gttttttgca gagacccttc caggagcggt
    14761 tcgtaataac cggtaaaaag cacacggctt tcatcatccg tcccggtgac ctgataaaca
    14821 tcgaatgctt cggccatcct ttttcccaat gtctccggat ccttcgcatc gcctaacagg
    14881 gcgcgaaaag cgagaagcgt ttccttgaac tgctgcgcgt caatctgcct gtcttcgatc
    14941 tgataaacgt tattgcgtcc gcggccctca taaaaacgga tgctgcggtc aatcgctgtt
    15001 tctagcgatg caggctccaa atcatcactg aaagcaacct gcgtggcatc cacaggaatc
    15061 agcgagtcca gcgttttgaa agacggttgg gaaacggcac gcatgcaacc gccgagaaag
    15121 aaagccatca agattagcag caggattttc agtgaatatc tcatagttcc acaatcatcg
    15181 ccacttcatc acagtaatcc gggtatttgg aacagcggaa gcaatccgac cagattttct
    15241 ccggcagcag gttccggtcg atttctttaa atccaagctt gagaaaaaaa tcttttttat
    15301 acgtcagcgt aaagaccttg aagagcccca gcgtaatggc ctcggagatg caggcctcca
    15361 caagcacccg gccgacgcct tttccccggt gggactcctc aacatacagc gaccggattt
    15421 ccgccaggtt tgcccagata atattcatcg cgcatatgcc gataattcgg gattcatcct
    15481 catcataata gacgaaaaaa tcccgcagag aaccatagat gtccatcaga gaacgcggca
    15541 gcatctcccc tttgcccgac gataaattga tcaggcggtg aatgattttg acgtcatcga
    15601 tgcgcgcttt tctcagcatg tgtctcctcc cgatactttg aggttatgtg agccggcgtt
    15661 ttggagcatt tcgcgggcat gctcccgcgt tgtcggtgtc acatcgacgc cgcccagcat
    15721 tctgctgatt tcttctattt tctgctcgct gtcaagctcg tttacaaaag tgagcgttcg
    15781 gcccttcacc acttttttgg aaacgaacat gtgtttatct ccaaagcagg cgatctgcgg
    15841 caaatgcgta atgcagatga cctgatgatt ggcggacaca gctctcaatt ttcttccgac
    15901 aatttccgcc accgcaccgc caatgccgtt gtccacttcg tcgaaaacca tggacaatac
    15961 cgacccggtg ccggagagaa catttttcag ggccagaata attctggaca attcccctcc
    16021 cgaagccacc ttatttagcg gtctggcgtc ttcgcctttg tttgccgaaa gatcaaattc
    16081 gatatcgtcg gctcccctag gcccgaagaa atccgtgttg tcgtcggcac ggtttttaaa
    16141 atgaacaaaa aaagaggcgt gcggcatatt caaagcgcgt atttctttcc cgactttgtc
    16201 ctccagcgtc ttcgccgctt tctttctgct ttcggacaga ttccgcgcgc tctgccggag
    16261 tctgcttttc accgcttctt cttcccggat cattttctgc aactcttcat ccagcgagga
    16321 gaccttccgg agggcttcct ccatgtcctg ttttttttga aaaacggctt ccagggaacc
    16381 gccgtatttt cttttcaaac gatggatcag atcaagccgc tcctcgatgg cggcaagccg
    16441 ttcggcatcg aaaaaaaggc ccttgccgta atcgcgcaga agcagagccg cttcctgcag
    16501 cgcgacaaaa ttattttcca tctcggcttc ggagattttt agaccggcgt ctattttttt
    16561 tatttcttta atctgacgct gaacttcttt taacatgacg atgacggagt ttttgtcgcc
    16621 gtaaagcaga tcaaaggcat tacccgccca ttcggaaagc ctctggatgt tgaccagaac
    16681 ctttttttca tccgacagta ccgtatcctc gttcggttgc ggattaatat cctgaatttc
    16741 cttcaactga aattcataca actccgttct tgcgtctgcg cttttgctca actcctctag
    16801 tttttcaatt ccagaccgga tctgctgata ctgattgaaa tgttccgtgt agaccgcgcg
    16861 ctcagacagg cagccgccaa actcatccag aatatcaata tgattttcag cagccagaat
    16921 gatctgatgt tcatgctgcc cgcaaatatt gatcaaccgt tcgctgacgg ccgccaaatt
    16981 ggccagggtt gccgcctgtc cgttgataaa aactttattt ttgcccgtat gggagattac
    17041 ccggcggata atcaactcat ccgaaacagg aaaacccatg gcgtcgattt tgtcccgcag
    17101 gggcttatcg gctgaaatat caaaaaccgc ttcgacggtt gcggtatccg cctgcgttct
    17161 gatcatctcc gacgttgctc tgtctcccag aagcagactc accgcgccga tgatgatgga
    17221 ttttcccgcg cccgtttctc cggagagaac gttcagtcct tcaccgaacg taacatgcag
    17281 ttcgtcgata atggcaaaat ttttaatgct caactcgtta agcatttttt cttttatgcg
    17341 ctcccgaagg taaatccccc cagccgagct tggaacgcag gatctctccg tagctgcggt
    17401 atggggaaag aatcagcatc gtggcatgcc ttgatttttt cacgatcacg acatcgccgg
    17461 attttatttt atgcgtttcc tgaccgtcca gcgacagcat tgcgcctttg tctttggacc
    17521 agagggtaat ccgaagatga gcatcctctg aaaaaataat cggtcggttt gtcagggtat
    17581 gggggcaaat gggattgatc aggatcaaat cttttccggg aaatacgatg ggacctccgg
    17641 ccgacaacga ataagcggtt gacccggtag gcgtggaaac gatcaagccg tctcccttgt
    17701 aagttgtcag atattccccg tccacttcca cttccagttc attcattctg gataaatcgc
    17761 cgcggttcat gacaacatca tttaaaacac tgccaatgcc ggccacggtt ttcccgtgtt
    17821 gtacactgac atcaagcatc attctctttt cggtcacaaa ttttcccttg agaatcaatt
    17881 ccatggtaga atggatttca tttaagttca cttccgtaag gtagccgagg ccgcccatat
    17941 tgatgcccag aataggaacc cggtgaacag ccagataacg cgccgagcgc agtatcgtac
    18001 cgtcgccgcc cagaaccacc gccacttccg ctttccttgc cagttcactc cagctgtatc
    18061 cgccgtccga aagaattttc ccggaaatct ccgtctccag aaacactttt gcgcctcgtt
    18121 cttccagcca ctttttcaaa gatcggacgt ggtctgcaat tttttctttt tcaatattgg
    18181 cggcaatgcc gatttttttg attttcattg atttcaccct tgaaatatga aggtccctaa
    18241 cacaaaaaaa aacctctgtc cacggataaa tagtctgcgg atgcatttat aaactgattg
    18301 atatttaaga gaaatccgga gtcattactt cttgacacat ccggcccgga taatataggc
    18361 tgcggcaaaa atggggattg aaaaatgggt ttactcaagg gtggttttac cttctcgcgt
    18421 ttcagcgtcg atggccagtt accgcaggcc tttttaaatt ttgtcaacaa ccggatccgg
    18481 gccaattctt ttaaaaattc attcaagtcg acggaagaaa aaaccatggg atgggtttct
    18541 ttaaccgaca tactggatac cgattttaag aatgccaatt attcactggg cgcctacctg
    18601 attttttcct tgcgcattga ccgcaagctc atcccgccga agctcttgaa gatcaaactg
    18661 atggaagagg aaaggcgttt tcttgccgaa agcggcaaga ataaaatatt ccggcaacaa
    18721 tcaacggctc tgaaagacaa ggttaaactt gatctgctgg ccaaactcga cgccatccct
    18781 tttttttacg atgtttgctg ggcggtggaa caaaatacac tttatttttc ttcactttcc
    18841 gacaaggtta tcgatgattt cgcggattta tttaaaaaaa cgtttgcatt gaatctgaaa
    18901 cgcttttccc cgcaggaaaa tcacctgatc agaaaggatg cggatccagc cgaatcgacg
    18961 tccttgatcg gccgcgagtt tctcacctgg ctgtggttta aatcggaaga acgtaacggc
    19021 agaatcgtgc tgccgaacaa agaggaatgt gaaattcatt ttctgaaaag agtttcgctg
    19081 gaagccggcg aaggcgaata ctctcagggt gtcgtctgtc acggcattca cgcagaactc
    19141 aaggaaggca aggaagcaat ccgccagggg aagaaagtca aggaagcggg aataaaattg
    19201 cttcataaca ataacgaatg ggattttatg ttcaaagccg attcgtttga tttccagtcg
    19261 ctgaaaatgc cgcaaaccga ctggcaggat acgccggaag acccgtcagg cagcctgctg
    19321 gagagaatct atctgatcga aaatgccgta aaaaccatcg ataccatcta tgaatctttt
    19381 cttcacatca ggcaatcgcc gcagtggaag gaaaaagaac tcaaactgct tcacaagtgg
    19441 ctggaaaaaa atcccaaaga ttaaatcgtt atttatcttc tcttttttta tcctcgccgg
    19501 ataaacgcgt gatgatccga ccgggaattt catccagcgg taactcatca caggccgctc
    19561 ccagtttaac cgcctctccg ggcatcccgt aaacgacaga cgtggcttca tcctgggcaa
    19621 tggtatatgc tttattttgc cgcatggcca gcaggccgtc cgcgccatcg gctcccatac
    19681 ccgtcaaaag aacacccatc gcattggctc cagcgtgttt ggccaccgaa tgaaaaagaa
    19741 catcgacgct tggtttctga tgatgaacca tcgggcctcc cttgattctg acctgataaa
    19801 atgctccgct gcgctccaga agcatatgat atccgccggg agcaatcaga gccagtccgg
    19861 gagccgccgt atcgccgtct ttagcttcgc gcacttcgat tttacacaac gcattcagtc
    19921 gctcggcaaa actggccgtg aaatacgccg gcatgtgctg gaccacgacg attccgggaa
    19981 tattcatggg cagccggctg aggatttttt caatggcaat ggtaccgcct gtggatgccc
    20041 ctatggcaat gaccttatgg gtcgtcttga gttgaatttt atatcccgga acgctttctc
    20101 tttccaccaa ttcaacagcc ggtttggcaa ttttggcccg tgctgccgcg cgtatagctt
    20161 ccaccaggcg tacggaaaga tctgcagtgg acaaactcga tcccggttta cagatgactt
    20221 ccacagctcc gagttccagg gctcgtatcg cgttctgact gtcgcggggg gtcagagaac
    20281 tgaccaccac aaccggcatc ggatagaatt tcatcagctt ggacagaaag gaaaggccgt
    20341 ccattctcgg catttcaata tccagggtaa tcacgtcggg cttaagacgg acaatcttgt
    20401 cacgcgcaac ataaggatca atggcgcctc ccaccacttc gatgtcttta tggcgggaca
    20461 actcttccgt caatactttg cgaacaagcg ccgaatcatc aacaaccaga actttgatca
    20521 atcgtttatc tcctggtccg aaatgtagtc atccacaaat aatttgatat ctgcccgccc
    20581 ctgattaaaa tttaaaatca cgacggccga aggtttactc ttttcctctt tcagcaattc
    20641 atccctgccg agatgtctgg ggctttgaat cttgtatcct cccgcttttc ttcgaccgag
    20701 cgacggcaca atgtttccgc agatgatatt ggcaatctcg ccgagggcat cttttttctc
    20761 ctggaggctt ggatgatcgc tacttaaaat attcgaagca atggctgaaa acaagccccc
    20821 tctgcttgcg atcaccaaac gtccgttgta agcgccatga aattcaacct cagcagcagc
    20881 atccggctcc agagcagcct ggctgtcttt gagttccggt tcaaggtaca taaaacagat
    20941 atcttcaaac gttttaatcg tggcttcttc cagaatcttt tcaatcttcg ttttcatggc
    21001 taacccccgt aacttcaacg ataacttctt taagaacctc cggtgtaaac ggtttatgga
    21061 taaatgtagc gcccaattcc agcatttttt ctattttacc cggatcgctt tccgaactga
    21121 tgacgatgac cggaaggttc ttatactgat cattttctct gacttttatc agcatttcct
    21181 caccgtccat taccggcatg tgaatatcga tcatcgccaa atccacccag tgatcctgta
    21241 aaacgtgcag tccttcttct ccgtttttcg cttcccagat atcacccaaa ggcagccccg
    21301 ccagtttcaa tgtttttatg atgatagttc gcataacaga gctgtcatca accaccagag
    21361 tatttaaggc catttcactc tctcctgtct aatctcttta gcatttcatt tctcattcca
    21421 agaaatcctt aaagttgttt gaggaaagtt tgagacttaa tcagcatctt tccgctttcc
    21481 aattccagcg tgacggtacg cgaaccgtaa ccgcccacgt cataagcttt cagcatgaga
    21541 ccttcattcc ataaatattt ccgtaaactg acaaaattgc ggcggccaat ctggaaaaaa
    21601 tcctcatttt cgttgagttt tgaacttgat cccccggcta cccggatgat cagatcgctt
    21661 tttcgtgaac ctttctgata aaatttatcc atcatggctt tcattccgct gtccacgtat
    21721 aaataaggat tgcctgctgc cttgttttgg tcgatcccgg aatccggcat catacaatgc
    21781 aacaaaccgc ccgtcttttt tctgaaatcg taaaccgtca cccccagaca gctgcccagc
    21841 gcatatgtaa tcaacatatc ggcactctcg tcggatatct tcaggtcggc aactccaact
    21901 gtatagacca tattacttca cgtccgttct aacctttccg gtttttttga cgaccgaatg
    21961 cgccttcctg atcttttgat aaacggccgg cctcatgtaa cgatactcgt gttggaggaa
    22021 gctcaggctt tccgaatgtc cgaccatgag atacccatct tcttttaaaa gatgccagaa
    22081 cctgcccacg agtttttcct gtgtcgattt gtcgaagtaa atcatcacgt tgcggcagaa
    22141 aataacgtca aaaggcccct tcatcggcca gtgttccatc agattgagct tggcaaaact
    22201 gaccatcgac tgtattttgg gaatcacttt gtatttataa cctgatcccg tatctgtttt
    22261 ttgaaaatac ttgtgtttca agtgacgcga cattattgcg agactttcct catcataaag
    22321 accctgccgg gctttttcca tcatcctatc cgagatatcc gtcgccagta ttttaatatc
    22381 gagtgtggcg gtatcaggaa tggattccaa taaaacgatg gcaatcgtgt acggttcttc
    22441 acccgaagaa catccggcac tccagattct gaccggaccc ttaacccaac cgggaataat
    22501 ttcgtgtttc aaaaaatcaa ggtgctctga ttccctgaaa aaattcgtct tgttggtggt
    22561 gaggatgtcc accatcgccc cgatttccgt tccgctctta tcgttggcaa tatgcttcag
    22621 gtactgatta aaatcatgaa atttcaagtg cctgagccgt ttgataagcc gggccttgac
    22681 cagttcttcc ttgccttcgt gaagatcgat tccggaaatc tgataaacca gtctgctgat
    22741 tttatcaaat tgctcggaag taagttttag agaataataa tccgtcgaaa gattattcca
    22801 ttccgaatgc ataatatctt ttcttttgtg ttggcagatt tctacttgtc cgaaaaaaat
    22861 ttcataacga ccggtacgaa ctagaaattc tgcaatatat catgatcctt atcatccagc
    22921 ggtatgacct tgcggggatc aggatgaaga ccggctgacg ctttatcacc ttttttccct
    22981 ggagcgacat ccggcagacg atattccgtt tttttaatgg catccggacc ggccgttaat
    23041 gcaacggtct tcgtcagtct gaatccggcg accatggccc gcatttcctc ggactgcgaa
    23101 gacatctcct cggcggcgct tgccgattct tccgcattcg ccgcgttctg ctgcgtcagc
    23161 tggttcatct gttccaccgc cttgttcacc tggttgatcc cctgatcctg ctgctccgat
    23221 gcggccgcga tctccgccac cacctgactg accttgttga tcttctcggt gatctcctgg
    23281 aaattcgtta aaacctccgt gttgatagtg acaccgttct ccgaatttct gaccgcctct
    23341 tctatcagac tggccgtgtt ctttgccgct tccgcgctcc gcatcgccag atttctcact
    23401 tcttccgcaa ccaccgcgaa tcctcttccc gcatctccgg cacgcgccgc ttccaccgca
    23461 gcattcaacg ccagaaggtt cgtctgaaat gcaatctcat ctatggtttt tacaatcttc
    23521 gatgtggcat ccgaagatga tttaatctga ttgatggccg atgacatccg gctcatgctc
    23581 tccacgcccc tctccgcact gccccgagcc tgttccgcaa cccctttcgc ctctctcgcg
    23641 ttcagggtat tctgcttcgt catggacgac atttcctgca ggctgctcga tacttcttcc
    23701 agagaactcg cctgctcgct ggctccctga gataacgact gcccgccggt gcttacctgg
    23761 acgcttgccg acgctacctg atccgcactg atcgcaacct gttgcagagc cccgtcgaga
    23821 ttgtccacga ccatattaac ggattccttg atcttggcat gaccgccttt atagtcgccg
    23881 gccattcgcg cggtcatgtc tttgtcggct accttcgtca gcaccagggc cgcctcattg
    23941 atcggattca tgacagcatc cagcgtatcg ttaaaagcct gaatgatttg acgaaaagat
    24001 ccctgataag gcgtgacatc cgccctgttt tgaagattac cttccaaagc gttgaggcaa
    24061 cacctcttga cttcattgct catcgaacct atggcattga tcattttaat aagtgcggga
    24121 ccgatttcat ccttactgtc cgtcactttc ggctcaacat cgatattgcc ctctgatatc
    24181 tgctgcatac tgtatacaat atttttttgc agattgtctg caaacatatc catggatcgg
    24241 cttaaactgc cgatttcgtc ctttctattc attttcaggc gtttagagag atggcctttt
    24301 tcaatctcct gcatcatcaa tacgattgtc ttaatcggcc ttgtaatgtt tctatgaacc
    24361 atcgtcccca aacctattaa ggcaagagcg ccgaagcctg aaaatatcca caaaataccg
    24421 gaccagccgc ccctgccttt gacgtactgt ttcaccctgg tttcgttttt gttgacactg
    24481 tattgattca agtttttaac taccgcaaga gcttttctgc ctgcttcgtt caaataactt
    24541 ttatcggcaa gaatactgtt tgcatcatca tttttccctt catccaccgc tttgattacc
    24601 gccttataac cgcgctggat ttcaggccac gctacctcaa attcggaaag atatttctgt
    24661 tcttcagaat ctctttttga gcttttcaag gactgaaaag aattctctgt agatgtgatt
    24721 ccttcctgaa tttgtatgag agcattttcc cgcgccgcag gggagttgac ataaagatga
    24781 agatccttct ccatattgac gagaccgacc tcgatggttc caatattttt tatatcgttc
    24841 acataatcac gatagtttga atcgagcata cgcaatgctt tcattcttac cgccgcacca
    24901 gatagcatca tcgccgttac cactacaaaa ataatagcaa atacaccggc aagcttaacc
    24961 cccattttca tgttattaat catttcgccc tccgattaaa gtttcactta tactatcttg
    25021 gtttaattca ataaaggcat cccctgtctg cagagaataa atttcttctt gttcacctta
    25081 aatgaacatt ctgtcagaag tcttttggtt cataggcaat gctttctctt tcagtatatc
    25141 ctgaacccgt gagtttaaat ccggcgacca tggcccgcat ttcctcggac tgcgaagaca
    25201 tctcctcggc ggcgcttgcc gattcttccg cattcgccgc gttctgctgc gtcagctggt
    25261 tcatctgttc caccgccttg ttcacctggt tgatcccctg atcctgctgc tccgatgcgg
    25321 ccgcgatctc cgccaccacc tgactgacct tgttgatctt ctcggtgatc tcctggaaat
    25381 tcgttaaaac ctccgtgttg atagtgacac cgttctccga atttctgacc gcctcttcta
    25441 tcagactggc cgtgttcttt gccgcttccg cgctccgcat cgccagattt ctcacttctt
    25501 ccgcaaccac cgcgaatcct cttcccgcat ctccggcacg cgccgcttcc accgcagcat
    25561 tcaacgccag aaggttcgtc tgaaatgcaa tctcatctat ggtttttaca atcttcgatg
    25621 tggcatccga agatgattta atctgattga tggccgatga catccggctc atgctctcca
    25681 cgcccctctc cgcactgccc cgagcctgtt ccgcaacccc tttcgcctct ctcgcgttca
    25741 gggtattctg cttcgtcatg gacgacattt cctgcaggct gctcgatact tcttccagag
    25801 aactcgcctg ctcgctggct ccctgagata acgactgccc gccggtgctt acctggacgc
    25861 ttgccgacgc tacctgatcc gctccgatgg ccacctgttg caaagctctg tccagatttt
    25921 caacagccag attaagcgat ttcttgatct tggcatgacc gcctttatag tcgccgacca
    25981 tgcgcgctgc catgtctttg tcggcaattc tctccagcac ggcagacgct tcattgatcg
    26041 gatcaatcac cgcatccagc gtccggttga cgccttgcac aataccctga taagctcctt
    26101 tatagtcctg ttcattaccg cgcacatcga gtttgccttc cgtggccgat ttggtcagca
    26161 tgtccacctc attgagcaga ctattgatgg tttcgataat ttttttcagt gccggtccga
    26221 tctcatcctt ttcgtcaata acgaccgggg caacattaat attaccctcg gatatctgct
    26281 gcatgttgaa tatgatattt ttctgaagat gatcggcaaa ttcatccatg gcattggcca
    26341 gcaggccgat ctcgtccctg cgtttaatgt cgagtcgatt ggaaaggtgt ccctttttca
    26401 tctccgccat catctgcacg ccttttttca gcggtttggt aatggattcg gtcagaaaga
    26461 cgatcgtgac aatcgccagg gctatcgcaa tcgccgataa gatcagcatg atgctcgaaa
    26521 gccccccgac attgtcaatc gttgctttaa ccgccgattc attcttattg acactgtaat
    26581 tattgaggtc gcgaagcgta gctgaggttt tctcccttgc ctgcatagca gcgcttccgg
    26641 cccctatgaa ctggtttatg tcattctgtc tgccttcatc caccgccttc atgccttcct
    26701 tgtaacggcg ttgcatttcg gaccacgccg attcaaaatc ggcaatcagt ttcttttcat
    26761 caacatcgat gcttctattt ttataggact gcatcgtgcc atccaccgaa ctgatggtct
    26821 cattgatttc cttactcatc ttctgccgat ccgccggtgc accaaagtat cgataaatgt
    26881 ctcctcccat tttttcgagg ctgactctga cattcccgat gtcacggata ttgatttcat
    26941 actggcgatg caccgaatcc actttgtgca cgacgtcgtt tcgtacttta ataccgtata
    27001 gaatcactgc cgccgctata agacaaacgg taacaatgct tcctatcagc ttaactccca
    27061 ctttaatatc gttcagcatg cttatctccc ttcctgtagc gtcagtttca aatcgttatg
    27121 gaatattgac gtcctataac tcggccatgg cgtctttgcc ttctccggca gcagccttct
    27181 tcatattgat aatatcggag gcggttatga ctttttcaat atccaggagc aggcggacgc
    27241 gtccgccggt tttgcccact cccaaaagat attccggatt aacatcgccg ccgaaagaag
    27301 gtgtatcctc aatatccccg gatgcaatat taatcacctc agaaacttta tcgaccagaa
    27361 tacccatcat catactggtg gtcttgatga caatgataca ggtctcgtcg gtaatctgag
    27421 tttcctccat gccgaatttg atctttaaat ccataatggg atggatttta ccccgcaaat
    27481 tgatgacgcc ccggatatat ctggacgtca gcggtacagg agtaatcggc atccggccga
    27541 taatctcctg aacctttaat atttcgactc cgtattcttc actggccaga aaaaaagtca
    27601 gatacttacc ggcccgggaa tcggcattgg cagattgatc gttttttact gcatttgcat
    27661 tgttcatgcg ttctcctcct gtttggcctc tttgttaagt tgttgtattg gtctgctgcc
    27721 gggcgatact gacgattccc gaggcatcca gaatcaaccc cacggttccg tcgcccataa
    27781 tcgctccacc ggctatgccc ggcacgttat tcattccttt gcccagcaat ttcgtcacaa
    27841 cttgggtctg ccctaagagt tcatccacga aaagagcgca gcgttgtttc ccttcgccga
    27901 tcaccaccag caggccggat gttaaatcgg taagggaatc agggatatcg aaaaagcgat
    27961 acagtctgat gacaggaacc atttcatccc ggaatgacag catttccgct cgctggttaa
    28021 ctgtgtgaat atcggattcc tgcggcctga ccgctttgtg aatatttgcc gtcggcaaaa
    28081 tatatcgttg ggagcccacc ttgacgatca tcccgtcggt aatggccatc gtcagaggca
    28141 tcctcatgat gaaggtacag ccctgacctt cagtggtttg aacctctatc cggcccctca
    28201 gggcttcaac ggcctgcttc accacatcca taccgacacc ccggccggaa acctccgtca
    28261 ccttttcagc cgttgaaaat cccggcagaa atatcatttg ccagacttcc tgatccgtca
    28321 tgcgttcggc agaatctatc aatccttttt gtatcgcttt attcacgatg gtctgacggt
    28381 tcataccccg gccgtcatcc cagaccgtca gcataatgct gccctcagca tggccggctg
    28441 tcagataaat gcgcccattt tctgattttc ctgcccggcg tctttcctcc ggaaattcaa
    28501 tgccatggtc aaccgcattg cggatcaaat ggatcagagg atcggccacc atgtcaacca
    28561 tgttgcggtc tatttcgata tcatctcctt cggtaataca gcttaccatt ttaccgtgct
    28621 tcagggaaag gtcccgggta agccgcacca ttttctggaa cgtgcccttt aagggcacca
    28681 tccgcaaaaa catgctcaag tcgtgaagtt cacggacgat ttttccggcc tgagatattt
    28741 ttttattcaa ctcatgatat ttgccgctga caatcagatt gtcctggcct accatggtct
    28801 gggcaatgac cagttcaccc acggtatcca gcaatttatc caaccggtcc acttttactc
    28861 tgaccgttga ctcggtcatc tgctcgcgaa tcttatttga ggaagcggat tgttccgacg
    28921 atgcctcccc ttcctttttt gctattttcc ctctgttcgt tttcgtataa gccggagccg
    28981 ccgcttcggg ttcgaccatt gattcctctg ccggaatcgc catttcctgc acctgctcat
    29041 cgggaacaaa gtttctcagt gattccagta atccctgata accgcggggg agttcgatct
    29101 gctggcccgg accggatgat ttcatttgtt ccagaatgct cttaatcaca tccagtgatt
    29161 tgagagccag attggcatag cttctcgtga aaacatgttc tccatctctg actttggcca
    29221 gcacggattc cacctgatgg gcaagatctt tgacacaatc cagattgaca aaggcggatg
    29281 ttcccttgat ggtgtggaat cctcgaaaaa ttgtattgag taattcctgg ttaccgggat
    29341 tctgttccca gtccagaatt gcggattcgg ccatcgttgc ccattccata ttttcgataa
    29401 tgaattcatt caataaatcc gcgtcaagat cggcggacaa cataacgtgc tggacgtcaa
    29461 tgctgtccgg ttcccgggca ggttctacct gagacgtttg ttccgtcgtt gcagaaatat
    29521 cggtttcatc gcttcccatt gcctgttcca gcaaaactcc cagacgggct ataatgttgc
    29581 gctgaagttc cggaacctgg tcgaccatag catccgcttc aaaacaagct tctttcaaaa
    29641 tattctgaat acgtccttgg gtattgttgt aacaggaaag aagatttttt ttaaattctt
    29701 ctaagtcggc aggattttca ggattcagtt ctatcaatga tatggccaga cgatttaagt
    29761 tcattttcga gataattgcc ccatcaaaaa ttaaatttta agaatctgtt tgacacaaaa
    29821 gagagtattc ccatgaataa acaatggctt tcattaatca ttacgttttt tacgtttttt
    29881 gtcaatatga ttgttatgtt cctaattcgt ttataattat agtatttccc cccattagca
    29941 caccgaaacc gtcaagcaat caaccgttgc gaatcgccga tagctgttcc gaaaatatta
    30001 atcatacatc atccaaaaaa atcttcatgc cggaagacaa acactattga aaaaatctat
    30061 agaatctgtt tataaaatat aatgttattt tatattgccg tatgttaaaa aacaaattaa
    30121 taaaattctt cttttccgat gaagtattat atggtaacga ctacacgcag aagaggtttt
    30181 tatgattaaa aaaattttga ttgtcgacga ttcaccgata tccgtaaaaa tcattaaaag
    30241 ttgcgttccg aaagatatgg ggtatgaatt gtacgatgcg gccgatggtc aaatgggtgt
    30301 ggaaaaatac aaggagatta aacccgacct cacctttatg gatttaacca tgccggtcat
    30361 gaccggattt caggctttgg aagaaattat gaaaatggat acaaaagcca tggtgatcat
    30421 tctgaccgcc gacgtgcaaa tcaaggccat tgccaaagca catgacttgg gcgctttttc
    30481 agtattaaaa aaaccgcctt caaaagaatc tgtcgcaggg gctatcaaag aagcagaaga
    30541 agccctggct tcccgtgggt aaattatgtc gcaaatggat aacattcagg gtttgattac
    30601 aagcgcagaa accgatcttc ttcaggagat tatgaatatc gctttcggcc gggccgcctc
    30661 ggacctggct gaatttatag atatttttgt tgttctcagc gttccgcaga tcaaattgct
    30721 gcctgcatac cagcttccgg actatatcaa tagtgaaatc atggactatg accaggatcg
    30781 ggtatccgtc gtcgaacaga gtttctgggg caaattcaag ggcaacgctc ttttagtttt
    30841 tccttccgga accggaaaaa ggatgatttc tctttttgat ggcaaggatg aattttttca
    30901 atcggatccc tcgctggaac aggaaaaaga aacctttctg gaaataggca atatattgat
    30961 cggcgcatgc atcggaaaac tcgcggagct tttgggcgac attgccacgt attcgcctcc
    31021 ccgcgttctc gttgaaaaaa gcctacatcg cgtttacgaa agcattatca accccgatag
    31081 tatggcgatc gttttaagaa cggttttttc ttttaacgaa aagaatatca gcggcttcat
    31141 gtttgtgctc accaaacagg aatcatttgc ctggctgaaa aatgcgttgc acaaattcct
    31201 cgaacaatac gaatagcatc ctgaaaagca aaaattgctg aagacgatgt atggaacatt
    31261 ctttcatgac tgacgcagcg gcttcataac ggtccccagc agtttcatct tgtgatgata
    31321 gtcgtctgca tccacgtctt tctgcagttt catccctgaa caaaatccgg acacgggcaa
    31381 acggctttgg aatcatgaat ctatcctgac tttgggaaat catgacgaca ggataataat
    31441 atcaatatcg ttaaaagaaa caaaccgtcg aaaggaattg ctgatgcaac ggacaaatca
    31501 gaatgtttcc tctgtgcaga taatgatcga tccattcgaa ttcattttct atcgtcagac
    31561 ggaaggttga gcaataaaat cggaccaacc ggaatatcat ttttccggcg gttacggttt
    31621 ctgccacatc acgcagcctc ttcaacatct cctttttccc gttttttgcg gttctaaaat
    31681 aatcatgaaa ggttttcccg tatacgtcgg aatttaattc agcatgctgt caacgcacct
    31741 ccgtctccgg aggtgtcgcg cagatgaatg ctatcaaata gggcatatcg tggtataata
    31801 agaagcataa aattttgaag cgtacattca acgatgcagt cccggaatgt cgattttgta
    31861 tataatcccg tgtaccgcta tgcgaaaccg gactctcgac aacttcctga ttcttgcctt
    31921 gattatggtt tcgcgattgt ttaaaaaaat tttagaaatt attttacaac gggcataaac
    31981 gatgacgcat ctcaaattag tcgaagccat gcagaaaccg gatttttatc cccatcgtcc
    32041 ggctaatgtg gaattcatcc agacgcatat ttcctatgtt tttatcgccg gcgattatgt
    32101 ttataaagtc aaaaaacctt taaagtttga ttttctggat ttcacaacgc gggagaaacg
    32161 aaaatttttc tgcgaagaag aattgaaatt aaacagaaga cttgcccccg acacatattt
    32221 ggatgtcgtc tcgctatcac gtaatgaggc gggcgatata acccttgatc agggcatcga
    32281 gatcgttgat tatgcggtgc gcatgaaaaa actccccggc gacaggatgc tcaagacgct
    32341 tctggccagg ggaacggcgg atgaaaaaat aatggatgcc ctggcccgta aaatcgtctt
    32401 gtttcatcag caggcccgaa cggacagccg catcgataaa atgggcgaca tcgaaaacat
    32461 tcgccacaat catgaagaga attttgcaga aacggaaaaa tttatcgatg tcaccatttc
    32521 cgaatatcaa tacagcttga ttaaagactt tgcaaataaa tttctgatcg agaataaaaa
    32581 acatttcgag cagagaatca ccgatcacaa gatccgagac tgtcatggcg atttacacct
    32641 ggatcatatc tgtatcaatg atgagatcac cattttcgac tgtattgaat tcaatgaacg
    32701 atttcgctgg ggagatgtcg ccgaagatgt ggcttttttg accatggaca tcgatttcaa
    32761 cggctggccg catcatgccg aaaattttat acaatcctac ttgaaatatt ccggtgatac
    32821 cggcctgctg aaactgttga atttctatcg gtgttattac gcttacgtcc gcggcaaggt
    32881 caccagtctt cgtctgctgc aagaggatac aactgcagac gaacaacggg acatcaccga
    32941 aacggcagaa cactatttcg atctggccta tacatacgcc gcccgactgg ataaaccggt
    33001 gctcatactg accgcggggc tgatgggatc gggtaaaagt tatcaagccc gccgtcttgc
    33061 cgtgcgcctg ggaacggaaa ttatccgtac cgacgagctt cgcaaagcat tgttcaacat
    33121 caggacgacc gaaagacggc atgagcgctt cggtgacggc atttattccg atgatatttc
    33181 ccgtctggtg tatgaaaaag ccctggcgct ggccgagcag aaaataaatc tgggaaaggc
    33241 tgtcatcatc gacgcttcct ttaaaaaacg gacggagcga ttaaaggccc tggcgctggc
    33301 taaaaaactt cagatccggt tttttgtcct ggaatgcttc tgtccggacg acatcaccca
    33361 aaaaagactt gaaaagagag ttctggataa cgataacgta tcagacggac gatgggaact
    33421 ttttcaacag cagaaggata attttgaacc tatcgatgag ataacggcgg attgccgctt
    33481 taagattgat acttccgaaa aaccggaagt cttcagacat gaaatgataa gaaaaatcaa
    33541 actggcggaa tttgaaa
//
LOCUS       DAONQZ010000019        33487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1433_length_33487_cov_14.656975, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000019 DAONQZ010000000
VERSION     DAONQZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 33487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33487
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1433_length_33487_cov_14.656975"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLR13_05105"
     CDS             complement(<1..866)
                     /locus_tag="PLR13_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC18746.1"
                     /translation="MIISILAVSTSFILLRLLLNPLVNFFNKAQKMPVIAKTQKDEQN
                     NISGDPSRMNEFFENVTTVLGKVEAEELFPDIVGSSTSMRNIFTQILKVAPTDSTVLI
                     SGESGTGKELIASSIHEHSERKDKPFIKINCVAIPEGLLESELFGHEKGSFTGATEQK
                     KGKFEIANRGTVFLDEIGDMPLNTQAKLLRVLQEKEFERVGGTKMIKVDIRFIAATNK
                     NLQDMIKHGSFREDLYFRLNVFSINIPPLRERKEDIILLANHFMKSLPVKLRLSNSAL
                     KGIIDYSWPGNVR"
     gene            complement(1114..>2169)
                     /locus_tag="PLR13_05110"
     CDS             complement(1114..>2169)
                     /locus_tag="PLR13_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQC18747.1"
                     /translation="KALGKSKVKPSDLVLFTKQFHSMLVAGIPILRILSILEFQSENP
                     SIREATVQIVKDINQGSSLSDALHKFPKIFDNLYCDMIRAGEISGNVPLVLERLIYII
                     EHEAKVKADIKSALRYPMIVIVALIGAFIGLLTFVLPTFANVFVRAGLTLPWPTRISM
                     SIYGVLANYWYLIFGIVLALIVGLHFYFKTSNGRFVRDSFLLDMPIVGPVFTKAALSR
                     FASIFAILQTSGVPIMQSLTILSATMGNEALTRAFDNVREKIKEGAGIATPLKSVKYF
                     TPMVIDMLAIGEESGKIDEMMRAVASHYDAEVEYAVKGLSDAITPILIVGLAAVVGFF
                     ALAIFMPMWDLTKMIKT"
     assembly_gap    2170..2269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2270..2489)
                     /locus_tag="PLR13_05115"
     CDS             complement(<2270..2489)
                     /locus_tag="PLR13_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18748.1"
                     /translation="MPTFQYTYEAVNQMGEEVSGKLEAESMENANSILVSRQLIPINI
                     SEQDAYGSGWLKALGKSKVKPSDLVLFTK"
     gene            complement(2505..4208)
                     /locus_tag="PLR13_05120"
     CDS             complement(2505..4208)
                     /locus_tag="PLR13_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQC18749.1"
                     /translation="MKTRKRLGEILVNQGILSEEMLAKVLSEQRKTNLKFGQYLIRQG
                     IVKEKQILDLLSDQLNIKKYQVNEYPLDFDLIRYIPFDYAQKNQVAPLRIKGNLLEVG
                     TADPLEIKVLDHIEKITNMEVEPVLCSEMEINQLISSMYGVQSDLGGIMDGLKIESKT
                     EDDTELQESLQITSLQDQAGEALVVRLTNSIFAQAVRSKASDIHISPRHNTVQLRYRI
                     DGKLIEMPSPPKSVFLSVIARIKILANMDITVSRVPQDGRFTMKTENKEINVRVSSLP
                     TIHGENLVLRLLDMSAGIYTLDSLGMDAQDMAKIESMCQKPYGMILSTGPTGSGKSTS
                     LYAILNAINKPDINIITLEDPVEYRVNDIRQVQLNTKAGMTFASGLRSILRQDPDVIM
                     VGEIRDAETASVAVQAAQTGHRLLSTVHTNDSAGVITRFIDMGIEPFLIASVLIVSFA
                     QRLVRTVCPYCRESYRPSDKALADFGITSREAENANFQRGKGCTQCNNTGYKGRTGVF
                     EVLAIDEAIQDMIVNQRSSPEITRAAVAAGKLKTLQKDAANKVMKGITTLEEAVAAVM
                     A"
     gene            complement(4225..5784)
                     /locus_tag="PLR13_05125"
     CDS             complement(4225..5784)
                     /locus_tag="PLR13_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18750.1"
                     /translation="MEYFNILNLKKEPFSNSPEPEFLFQLPQYSDCLQKLELAVRLRR
                     GINVIIGDIGTGKTTLCRKLIQNFSPSSKDAGKTEIEPHLLLDPAFKSNLEFLQTFAS
                     MLGINDSEPETESEWHLKEKIKDCLFDKGVDEKKIVVLMIDEGQKIPDDCLETLREFL
                     NYETNEFKLLQIIIFAQKEFYDILKKKANLLDRVNLLYRLRPLNFKQMKAMINYRLKV
                     AGHFEKHPPVFSFSGFLAVYLATGGYPRKVVFLCHQVLLKMIIREEKKAGWFLVRSGR
                     ENMKAPLSRKLGWGLAGLLFLIVLGISAGAVALQGLHSGKEKQGRLASTGSGINESAP
                     AMSVVHPSGDSTVQAMDVPDEKMPDALGNISMAKRRTLWWVSYNIYGDNTPQFMEKIF
                     AVNPHIKNKDVVAEGTIITLPAVPADSSPLNPGDILIALESGQDLEIMYNLFRNHPHE
                     QILPPLAFLSLWNKKKGFEFAVVINQCFKNEEAAWQAINKLPPSLAAKAKILSQWDAS
                     TVFFNRRAPEY"
     gene            complement(5920..7443)
                     /gene="pilQ"
                     /locus_tag="PLR13_05130"
     CDS             complement(5920..7443)
                     /gene="pilQ"
                     /locus_tag="PLR13_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HQC18751.1"
                     /translation="MTLVFCCTALMFVWGCAGGVKNEKKDAFFEKWTTLAENSQGHSP
                     SAHPKKIDTSALIAKKDPASPMVGASDEAKKLPTSPIHLTMRQADLKAVLRAMAKSVN
                     KNLLVKNELKGEISVDFKGVAWDEAFTGLLRTYGLSYVWEGDIIRVMTADDLKQELER
                     KVQMRDIQWVEPLLEPVVVSVDYADSKKLAETAQELLTKDKDGKPRGSIKVDDHSNSL
                     IISAMRDDLTKILPIIEKLDKPTSQILIKANIVETTKGVARDLGVMWGGYNRSTQGKE
                     DLIITGGGVSPAGPIGGTGTAPQRSGLGVNFPVDTTNLSSVIAPMGSLGLLFGRIDGN
                     LLEVQLQALQQDNKLNILSSPSITTLDNQEASTKNGERIPYVTTETSGGTTTQTTKFE
                     DVVMELKITPHVIDGKNLKMKILVKKDEVDMTRRSSMGDPYIIKKQTETELIVKDGET
                     IVISGLTKQTNTYSQSGVPWLKDIPVLGWAFKSDGKGENMEEVLIFITPHILPVGNS"
     gene            complement(7499..7999)
                     /locus_tag="PLR13_05135"
     CDS             complement(7499..7999)
                     /locus_tag="PLR13_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18752.1"
                     /translation="MAKLKKREIVILAIAALFVLYAVYVYLIADRQKGKKVKPDQETV
                     KVEAVVSGITGELNKSKISDFDHYVIQRTQTDWDKNPFLKRELYRAWLAKDSKGKDGV
                     AAVPIIYSGYIETGKNRLAVLNGIEYRVGEALQEEGFVLKKIMPSKVVIFDKRIGSNL
                     EIPLQE"
     gene            complement(8016..8555)
                     /locus_tag="PLR13_05140"
     CDS             complement(8016..8555)
                     /locus_tag="PLR13_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18753.1"
                     /translation="MMKNFNLNAQQKKSILYAVICSGIVLIIVLVGILPYSFKVSNQQ
                     NENEKLTSQIKEQRELGPVYKTLTGNVEQTDVLILPHAQKTALPRQDITIIQKDFQAA
                     AQKSGLKIISYAPEINTSASPSASFLHNLELKGELAGFRKLLIALGALPYVERIEEIS
                     IQQNAGAMDFKMKIWTAVQ"
     gene            complement(8555..>9756)
                     /locus_tag="PLR13_05145"
     CDS             complement(8555..>9756)
                     /locus_tag="PLR13_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18754.1"
                     /translation="GITIVPFAMQNIFRSKWIPASEEIFASLFIGDNYSRIDVYDKEN
                     LVMTRGIKTGSLSSMAEAVVAGFSGRKGDLKLDQTEAKKIVCTMRPESGGAKEADDRQ
                     KFAREDILNMIAPVWERLARQIDLTLKTSPIGNKKVEKMYVLSPINFDQSLLDYMGNQ
                     LDTRIEIFDPFKNRKSVISEQSLSSSERILISPALGFALSDNSYTPNAIFTFEEKRQD
                     IKSKRNNKIIFMAFLAALMVCIGTLFYQGTKFSTLKKNNLALEKELALFSPMVSQETI
                     LQEVNRIKLQRDAVRQYAQKYLGVAVIGEISDTTPENVRLINLRLRQAAGSPAAAGQK
                     TEHNGLVLEGIIFNKKDMLESDLTQYVLKLENSPLFNTVSVQNKNIVNFDKKDVIHFV
                     LSAKLGS"
     assembly_gap    9757..9856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9857..10545)
                     /locus_tag="PLR13_05150"
     CDS             complement(<9857..10545)
                     /locus_tag="PLR13_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18755.1"
                     /translation="MSNTSKDINSTEKLLNVIRGKDEASFGGLGKQKISLTPDKKTGI
                     FQSGFLEKFLSKKKYTVGIDIGREFICLVKTAQDSNHQPILVDKKIITIDPRFVIGSP
                     EFNQLVKTSVLNFCGNVADCDIWTKISTSQVGVYFFTIPRVPKKQIQKVIFWTAKKEG
                     FYDEEKQIFDFEIHGELVEQGTPKYSVMFYTAQKSEIARIESFADNLGINLKGITIVP
                     FAMQNIFRSKW"
     gene            10777..12174
                     /locus_tag="PLR13_05155"
     CDS             10777..12174
                     /locus_tag="PLR13_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQC18756.1"
                     /translation="MNKKKKIIISCLILLLIGALFYFMNIYSTVAADIKLRGSSSPPL
                     TEKKQIAKPASSDHSSYKGQIPDSIVAFSSGYAVVVDKEHQKLYVFYKSGKYSKVFET
                     VCSTGKNPGSKRIAGDAKTPNGIFFATRILENPGPSETYGSLAFTLDYPTLSDKRAGK
                     DGNNIWIHGTTKPLLPQQSNGCVVLSDEDMKKLANFIHLNRTPVIISESINWVDQDYL
                     SDSRDEFEKILWSWNKAFVEKNLKEIDSLYAEGAQIRGKKREDLHYKIKNLDFIKKHF
                     VLQPRDIAILQEGDNAVIIFDQIFDVKDNNTFQGFYNKLVLEKINENWYVVDESSISE
                     PADRRLATAKDTQKDAVSPEKIAEKNIRSLVRKWRASWESGSMKTYRACYASDFQSRN
                     MGLDAWIAHKIKVRQKSGNIKISVNNLQISMSGNTARATFVQQYSSSILRTKGSKTLE
                     LKKSADGWKIHREIM"
     gene            complement(12375..12848)
                     /locus_tag="PLR13_05160"
     CDS             complement(12375..12848)
                     /locus_tag="PLR13_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC18757.1"
                     /translation="MSYTITEKCNGCGACARVCPADAITGEKKKIHSINAALCIECGA
                     CGRVCPQGGVLDADGVLCVMIKRSEWPKPEIIVGKCIACGICVGTCPAGCLAMSDRPR
                     NQGVDAFPYLKDAKSCIGCGFCALECPVDAIVMQKPEKAKKESAEMKAAGSAASA"
     gene            complement(12860..14815)
                     /locus_tag="PLR13_05165"
     CDS             complement(12860..14815)
                     /locus_tag="PLR13_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQC18758.1"
                     /translation="MSKAYMGKILMVDLSSKTMTEETIPDSVYQQYLSGMGLGAYILY
                     NRIPAGADPLGPDNILGFCSGLLTGTGSLFSGRWTLVGKSPLTGGWGDANCGGTFSPA
                     IKHCGYDAIFFKGISPNPVYLYVKNGKTFLKDASDLWGMDAVETEEKLKKQYGERAGV
                     AVIGPAGEKVSLISGVCTDKGRIAARSGLGAVMGVKKLKAVVLDGVQRIQAQDPKSMK
                     SLSQKCIKWTNFPMPLGLLPGVGLALMGTFMGATPVMFAQDGLLYKTILRTWGTGGMN
                     QMSIEMGDSPLKNWEGSSRDFHLGKSKTVNPDIIKKAEIVKYHCYSCPLGCGGICATK
                     GKYSHTHKPEYESILALGGLCMNEDLDSLFYLNELLNRAGMDTISAGATVAFAIECYE
                     KGILTKEDTDGLELKWGNTEAIVALIRKMVDREGIGDILADGSKVAAAKIGRGAEECT
                     VHAGGQEPAMHDGRGDPGFNVHYSVEPTPGRHTIGAHLYYEMFQLWKRVKGLPKPNLL
                     YMKGSKYKINKEKAEASAACSKFMSMGNGAGMCMFGLFIGAKRIPTFDWLNAATGWTF
                     TPEEYMTIGERIQTIKQNFNIRHGIEPRKNFISSRAQGKIPQKKGPNKGRFVDVEKLA
                     ADYWEQFGWDRTNGKPKEETLKKLGIK"
     gene            complement(14837..16084)
                     /locus_tag="PLR13_05170"
     CDS             complement(14837..16084)
                     /locus_tag="PLR13_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC18759.1"
                     /translation="MILPDYYEFCCRVKIVAGHDALEKIPALLAEMGSKKPLIITDKG
                     VAAAGLVDIVVNALKDGVTIGGIADNVPPDSDLKVVNQLAGLYRDNACDAIIAVGGGS
                     VMDTAKGVNIVVSENATDLMAFSGAGALNRRLKTLVAIPTTAGTGSEVTLVAVIKDHE
                     KHLKMAFTSYFLLPSVSILDSRMTMTMPPFITAMTGMDALSHAVEGYYCLQKNPLSDA
                     HSLAAVNMIHHYLPTVTKNPADKDGRLAMAVSACLAGISFSNSMVGMVHNLGHATGSV
                     CGVPHGICMAILLPYGLEYNMHKRGDTIGELLLPMAGEDVYLKTSKADRADKVVSLIR
                     QMNQDLNEITGGRHARFFSEIKDRDGKQMVPREILPEIAKAALLDGATIYNPEDADYE
                     DNLLVLEAAWEGKPLDRNKIKKG"
     gene            complement(16103..17260)
                     /locus_tag="PLR13_05175"
     CDS             complement(16103..17260)
                     /locus_tag="PLR13_05175"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC18760.1"
                     /translation="MNITNGFAFICPFKTNSGNKALEHLPVELAGFNAFKPLIITSRQ
                     LAGWRAISTLKNAFGDSGMTLGLFDDVTQTADLNVVEQIRDIFVKGQYDVIIALGGGI
                     IVDVAKIVNLAVSQKVADACQLSPATAIRDPLGPLVVIPTFAVNGMETSKYAVLSGKT
                     FTSVHLMPDLVVLDPRLTRAKDGKTVAEAGIAAFGRTLEAYIAPDKNPFMDAYAFTAM
                     RFIRENLALAVKKRCNKKESLAVANAVAMSGCVISNTDQNVLHRLGQVFQNTVHVHPG
                     VIIGMCLYPLLDDTMKKGNSLVSNLLLLLGGDDKYAAMPEAGRTGAALREVYGFTYEI
                     YSMLKGKMPQTIREAGIPQYLMDDVLEVINQEPDGTYLRTVIDRVGGSTKA"
     gene            complement(17276..17797)
                     /locus_tag="PLR13_05180"
     CDS             complement(17276..17797)
                     /locus_tag="PLR13_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18761.1"
                     /translation="MREIHAGGKLLKKIYVAIFLWFTGRAIQAAAKVDKEVQKEFDAL
                     PDEFTFALGCLPNGPWMVVGKQKGKVKYFGGDLKCRPVHLRLVIKSIDAAFLILSFQE
                     STALATTRNRLMTDGEVPYALSVIRILDIVEVYLLPKIIASLAVKRYPSWWSFGRKLA
                     GRVGVYTRTLLGI"
     gene            complement(18149..18571)
                     /locus_tag="PLR13_05185"
     CDS             complement(18149..18571)
                     /locus_tag="PLR13_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18762.1"
                     /translation="MAQINFAPGVEDAGLPVMIADIIKGNLEAKPQRQGDFNALRGNI
                     YMQAHDAEVDMTLVFEKGSLTVHSGKVGDPKISISTDSDTLLALANIDVKFGLPYYFD
                     KVGFEVLKKLFLSRELKLAGLLTHPIMLTRFTKLMSCR"
     gene            complement(18615..19778)
                     /locus_tag="PLR13_05190"
     CDS             complement(18615..19778)
                     /locus_tag="PLR13_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC18763.1"
                     /translation="MAYDRDLNFIYRNPTRLIYGENSINEVGLEADALKCSRAFLVTD
                     KGIVNAGLTERVEKALGRKLVGTFDGCIQDSDLRIINDAAEIARGKGADILVSVGGGS
                     VIDTTKGIAIVLKEGGKIEDYKGQQCLTRPQTPHIVIPTTAGTGSEVTYFAVIKDQVN
                     HQKHPYCDDNIIPNVGILDPTMTAGLPPMLTATTGMDAFSHALEAIHATPCSPVADGM
                     ALHAIRLIMEYLPLCVENGKDLFARGQQLMASTIAGIAFTNAQVGLVHAIAHTLGGLF
                     QVPHGLANSLMLPHVVRYNMDTCADKYVMIAQAMNIYAKGMSDEEASEAVAEAITALT
                     KRMGVPQRLRDVGVPEDGLVQLAEGTLSDGAIVYNPKPVFEAEEVLGIIKQAW"
     gene            complement(19908..20486)
                     /locus_tag="PLR13_05195"
     CDS             complement(19908..20486)
                     /locus_tag="PLR13_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQC18764.1"
                     /translation="MPYTYGTPEWDEAYNKRVEERLANEPKPFIIFLPEWLKEWEKYM
                     QNDAKYKEVALPNWENAIVIHITPAPQFGLDHDIFVRMDLWHNGECRSIRYIPKSEVG
                     KNPKDFIITASIDRWFAVGRKQLDVVKGMMQGKLKLKGDLPTVVKAVKPAVRIVETVG
                     EVGGKYEDELTPEETEKFRATVNELLPEFGLV"
     gene            complement(20571..22448)
                     /locus_tag="PLR13_05200"
     CDS             complement(20571..22448)
                     /locus_tag="PLR13_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQC18765.1"
                     /translation="MANCESGYAKKLLEINLTEVKIQKAPLDESLLRNYLGGGGLAAH
                     LFLNRFTPDSDALSADNPLIIMTGPLVGTLLPGTSRFAVCARSPQTGFWGIGTCGGTF
                     GPELKFAGYDGIFIEGKSEKPVYLLIEDDNVEIRDAADLWGEPVYKVTDILKERHGGK
                     RKPKVLVIGPGGENLVRFAAIANDKAHYIGRTGMGAVMGSKMLKAVVVSGTGKVLPAL
                     PEEYKALRENLLEKIKENQFGQVLRSMGTAASMDPGMMMGDVPIRNWTKGEFDGSVNI
                     GGPTMTEKYLTKVHACYACPIACKRVVRVEGGPFQTEEGPGPEYETCCTFGSFMENDN
                     LEGIIKANETCNQYGVDTISCGATIAFAMQCFDSGILSTEDTDGIELNWGNIESALKM
                     VEKIAKREGIGNVLAEGSAKAAQIIGKGSEQYVVAVKGLELPAHDPRGFHGMGLEYAV
                     GYRGACHLQHMSHTIEQGMTDQADAGFKADYEGPSSDGKAEMVVLSQNIGVPPTSACL
                     CLFVYDCIAVKDFADMIHVVTGWDYSIDDLIKTGEKIWLLMRGITNLLGSRADDDTLP
                     PNVMKPLEEGMTAGSVPDIEKMLTEFYELRGLDASGLPRRDVLEKAGLNDLAQKLHGA
                     A"
     gene            22895..24235
                     /locus_tag="PLR13_05205"
     CDS             22895..24235
                     /locus_tag="PLR13_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18766.1"
                     /translation="MKKILFTLLMVFLFFTPSIFAEMVQDIPAGTAEDYYNRGNKYRE
                     QGNLDQAIYDYSKAIKANSKYSKAYYNRANSYVKQGKLAEAIEDYTKAVEINPLYTEA
                     YYNMGNTYEKQGDLAQAVASYTKALETNPRYSAAYCNRGNVYQAQGNFQQALDDYNKA
                     VELNPNFAGVYSNRGNVYQAQGNTDKAIADYNKAIELSPNFAGFYANRGNIYQSQGNL
                     QQAIADYNTAIEKNPDDTSSFYNRGLAYYGLEEYAKSLADYSTALNQNPDKEAYDDFL
                     KYFPEKRTSDNQDIRGAIVQVFEDKLNLPKKIAAPVAAAPADAIPPAAAAAVVADTKV
                     SSTPEEDIKGSVNTWLNSWQSGDMATYRSCYDERFESRKMNLDAWIDYKNNVRNRSKN
                     ISIQIDDLKISVDGDRAKAVFTQSYSSSLLKDKGTKTLELKKVGDNWKIYREIM"
     gene            24351..24971
                     /locus_tag="PLR13_05210"
     CDS             24351..24971
                     /locus_tag="PLR13_05210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18767.1"
                     /translation="MNISKQKIDQVKGFLDEREAACLYRLALEAGKKGPCLEIGSYCG
                     KSSVYLGTACKENGTVLFSLDHHTGSEEQQPGEEYFDPDLLDRESGKIDTFKFFRKTI
                     FDFDLDDVVIPVVGRSATIGRVWKTPLSLIFIDGSHAYDSVLSDYEIWGKNLMPGGYL
                     LFHDIFSDPAKGGQAPYRVYQQAVDSGRYTEMPMCESLGILIRKQE"
     gene            complement(24974..26548)
                     /locus_tag="PLR13_05215"
     CDS             complement(24974..26548)
                     /locus_tag="PLR13_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18768.1"
                     /translation="MRIAVIAPPYPLEEMPSPPLGVTYVAAAFAGAGADVRIFDYIVS
                     GYSREKIENQLASFSPDAVGATSVTLNFYDAQKILRDVKQYNHEIITIMGGPHVSFTV
                     EETLKHYPEIDLIVIGEGEATIAELTPVLRQKEKWREVRGIAYREGDEIVRTPKRDFV
                     TDIDNIPLPARHLLPISRYRALGFPVSLITGRGCPYSCIFCLGRKMVGSKVRKRNSRL
                     VLDEIEQIIGYGFDRINIADDLFTTDKERVREICAGIKERSLKFPWSAFARVDTVSQE
                     VFDLMAEAGCDSVSFGVETGNPDMLKRIKKGIRLEQVYSAVKMCQQAGMIAHASFIIG
                     LPGETKETLHQTDQFAKSTKAIYGYHFLAPFPGTTVREKIGDYDLQILTDDWSRYDAN
                     DAIVRTSFLNPEEMKAFGDGYEKEMTDDWNKIVRRYHEGRGSVEDKMQVEGHWRMKLT
                     YRLLKEDIIEQCGLIEPECCRGSKENALRELCNRIGKITQADPGVIDNTLYDFAGRGY
                     LKAEMSDQGCRWSWSK"
     gene            complement(26552..27274)
                     /locus_tag="PLR13_05220"
     CDS             complement(26552..27274)
                     /locus_tag="PLR13_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC18769.1"
                     /translation="MRKDHRPYYIKRFYNRFQKWYARYYIAPQCEYFGRGLVFMKPWH
                     VEIFGGPVSLGDYTTVIATSDRKVRFTVWPVWMGQGKIEIGQCCLICPGTRIMSATSV
                     TMGDGCMTAQSVSIGDADWHDIYDRSMPVGNTQAVTIGNNVWIGDSAMVSKGVTIGDN
                     AVIGAGSVVVKDIPANAIAAGNPARVIKYLDPDRPIRTRKDWLSDPAGLARDFEVIDR
                     AIMKGNTMAGWFRSVLFPRQGD"
     gene            complement(27369..28403)
                     /locus_tag="PLR13_05225"
     CDS             complement(27369..28403)
                     /locus_tag="PLR13_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18770.1"
                     /translation="MELELSVSEASAPVSVEKIGSFIISLQKENGEIPWSAGGKTDPW
                     DHVESAMGLSAAGFYKEAERAYEWLASTQLEDGSWWSEIQDGKIINSCKESNFAAYVA
                     VGVFHHYLVTRDEKFLKKMWHTIAQGIQFAINLQAPDGEIYWARNKHGAVDKMALLTG
                     SSSIYMSIKCALAVADRLGKKRAGWRKAMDALGAAIKNRPDLFNMIKSRYSMDWYYPI
                     LCGAVTGAEAQKRIAHLWEKFVVPDWGVRCVSDRPWVTMAETAEFILTLASIGDLARA
                     KTIFSWLHDKRFSDGSFWMGVTFPDGVIWPEEKTGWTAAAVILASDALNEMTPAGKLF
                     NHQFWASISK"
     gene            complement(28409..29110)
                     /locus_tag="PLR13_05230"
     CDS             complement(28409..29110)
                     /locus_tag="PLR13_05230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC18771.1"
                     /translation="MITVEIDKLDLKPDSVVLDAGCGLGRHLRHLARIPELKIFGIDK
                     NTWALQETAKSMDTQPDARSKDYLVSIADINRLPFADASFDCIICSEVLEHIPDHQKA
                     IQELDRILKPNGTLVVSVPRYFAERICWFISRDYHNEEGGHIRIYRKKQLHRMLTGQG
                     FKCWKINYKHALHSPYWWLKCLVGLKNEDHPLVRSYRKFLEWDIMKKPRPVRMLEELL
                     NPLMGKSIVYYLRKG"
     gene            complement(29091..30350)
                     /locus_tag="PLR13_05235"
     CDS             complement(29091..30350)
                     /locus_tag="PLR13_05235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQC18772.1"
                     /translation="MKGKSKSQPLKICLLTYRGNPNCGGQGVYIKYLSKALSDLGHEV
                     DVLSGPPYPHLDPPVRLHRLPSLDLYNVEHPFRVRNARALMNPLNMYEFLTMCTGRFP
                     EPYTFGVRAYNYLQRHRKKYDIVHDNQCLAYGIGKIAQNVVPTIATIHHPITVDRQEE
                     FKSATSKRKINKIKKWYSFIRMQKEVARQLSHIVTVSECSKIDIAEEFSIPSSKFRVV
                     PNGINKEFFYPVQNGSRPQNSIIVTNSADTPLKGLRYLLEAVYRIRKKQPVKLTVIGE
                     PKKNGVVKKLVAELGIEDIVHFTGRIANEEFADYYSKATIAVVPSLYEGFGLPAAEAM
                     ACGVPLVSTSGGALPEVVGDAGIIVPPADADALAREILLLFNHPDQRKKMAQAGIARV
                     DAIFNWSKAAGDMVEVYREAIHDYRGN"
     gene            complement(30340..30996)
                     /locus_tag="PLR13_05240"
     CDS             complement(30340..30996)
                     /locus_tag="PLR13_05240"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HQC18773.1"
                     /translation="MIDLHTHSIFSDGELIPAELAQRAWAAGYKFLAITDHADHSNID
                     FIIPRIIRACFKINQAGKINVFPGVEITHVAPAQIAGLANEARKLGAKVVVVHGETIV
                     EPVPAGTNLAALKADIDILAHPGLLTEKEARLAAERGIYLEITTRRGHSFTNGHVAQT
                     ARKCKAKLMLNNDAHAPADFAGRERAQRIASGAGLNAREVSAMFENSRRMVKKLQYER
                     "
     gene            complement(31109..31540)
                     /locus_tag="PLR13_05245"
     CDS             complement(31109..31540)
                     /locus_tag="PLR13_05245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQC18774.1"
                     /translation="MIVNYDVYLEKLKAGQKGINPFLDFLGAALVELKDGYACFKMPV
                     RPEYLQGAKAMQGGLMVALADETIAHAMMTQLTPEEGLTTIELKSNFLAGVSGGELTA
                     TATVFKKGKSLMIGDCLVTDNKDKNVCRVSATFLLLKEKSK"
     gene            complement(31613..32689)
                     /locus_tag="PLR13_05250"
     CDS             complement(31613..32689)
                     /locus_tag="PLR13_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18775.1"
                     /translation="MMKVENIVLRRGGVVVLAIPEFHVQPGRILALIGPNGAGKSTLL
                     LMMAGLLKPQQGKIYFQGRAMENRADLNFLRRNVSVVFQEPLLLNSNVFDNIALGLKF
                     RKMSRDEINNKVQKSLEAFGISHLEKRYARALSGGEAKRVSLARALAIEPQMLLLDEA
                     FNSLDPPSRETIIEDLKQILEKTKMTAVLALHDREETLRLAQDVCVMHAGNIVQSGTT
                     ARIFNQPDSEFVASFVGTETILEGWVRNCTAGNMLVDVEGRQIEAIGDCSIGRKVFCC
                     LRPENVIISSRTQEEVSVRNSFEARVSKIIRQGFFNKLILDCGFPLVAYITIPSCEDL
                     GIAPGVTVVASFKATSVHVIPREK"
     gene            complement(32701..33399)
                     /locus_tag="PLR13_05255"
     CDS             complement(32701..33399)
                     /locus_tag="PLR13_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC18776.1"
                     /translation="MELIIQGIIKAFELIVTFDPEVLGITWLSLKISGTATFISLFIG
                     VSIGTAVALNNFFGKRFVISLINTGMGLPPVVVGLFVTMMIWRSGPFGFLEILYTPYA
                     MIIAQAIIATPIVMGISLASIQNLPPNLRLQILSLGASRLQMVWILIKEAKLPLLAAV
                     MAGFGGVISEVGASIMVGGNIKGYSRVLTTATVMETGKGNFDIAIALGIILLILAFMI
                     NALLTQIQQRQRPR"
BASE COUNT         8352 a         8635 c         7457 g         8843 t
ORIGIN      
        1 cgcacatttc ccggccagga atagtcaatg atacctttta atgctgaatt ggaaagtctc
       61 aacttgacag gcagggattt cataaaatga ttggccaata aaataatgtc ctctttccgc
      121 tctcgaagcg gcgggatatt gatggagaag acatttaaac gaaagtaaag gtcttcccga
      181 aatgaaccat gtttgatcat gtcctgtaag tttttgttgg tggcggcaat aaaacgaatg
      241 tccaccttaa tcattttggt gccgcctacc ctttcaaatt ctttctcctg caatacccga
      301 agaagcttgg cctgagtatt gagcggcata tcgccgattt catccagaaa taccgtgccg
      361 cggttggcga tttcaaattt tccttttttc tgctctgtgg ctccggtaaa ggaacctttc
      421 tcatggccga acaactcact ttccagcaac ccttccggta tggcaacaca gttgattttt
      481 atgaacggtt tgtcttttct ttcgctgtgt tcatgaatgc tggatgctat taattccttg
      541 ccggttccgc tttcaccgga tatcaggacg gttgaatccg ttggcgcgac ttttaaaatt
      601 tgtgtaaaaa tgttgcgcat cgacgtgctg gaccccacga tatcgggaaa tagttcttct
      661 gcttcaacct tgcccagaac cgttgtcaca ttttcaaaaa attcattcat ccggcttgga
      721 tctccgctga tattgttttg ttcatctttt tgtgttttgg caataacagg cattttttgc
      781 gccttgttga aaaaatttac aagagggttg agcaacagac gaagcagaat aaaacttgtc
      841 gaaacggcaa gaatagatat tatcataata atataaagca gattgataat atctttccgg
      901 ccaaaatgat cctgagaatg aaataattga taggttagga taacaccaag aatggctatg
      961 cctgtgtaga tcaaaggaac taacacatat aagctgatat aaactttctt ttttttcatt
     1021 ttaacctttg ggattaaaaa taaaaagaga cgtccttaaa ttttttgccg gctgacatgc
     1081 gaagattcac tgataaattt ttgcacgttt catttaggtt ttaatcatct tggtaagatc
     1141 ccacatcggc ataaagatgg cgagcgcaaa aaagcccacg accgctgcca ggccgacgat
     1201 cagaatcggg gtaatggcat cggaaagtcc cttcaccgcg tattcaacct ccgcatcata
     1261 atgcgaagca acggcccgca tcatctcatc tattttgcct gattcttcgc caatggcaag
     1321 catgtcaata accatcggtg taaaatattt aaccgatttc aacggggtgg caataccggc
     1381 gccttcttta attttttcac ggacattgtc aaaagcacgc gtcaatgctt cgtttcccat
     1441 ggttgcagaa aggatggtca gagactgcat gatgggaacg ccgctggttt gcaatatggc
     1501 aaagatgctg gcaaaacgcg acagagccgc cttggtaaaa acgggaccga cgatgggcat
     1561 atccagtaaa aacgaatccc ttacaaatcg tccgttactg gtcttgaaat aaaaatgcaa
     1621 cccgacaatc agggccaata ctatcccaaa aatcagatac cagtaattgg caagaacacc
     1681 atatatggac atggatattc ttgtcggcca gggtaacgtc aaaccggccc gcacaaaaac
     1741 atttgcaaag gtgggcaaca caaaggttaa aagaccgata aaggcaccga tcagggcaac
     1801 aataacaatc atcggatacc ggagagctga tttgatatca gccttaactt ttgcttcatg
     1861 ctcaatgatg tatatcaatc gttcaaggac aagcggaaca tttccgctta tttcaccggc
     1921 tctgatcata tcgcagtaaa gattgtcaaa aattttgggg aacttatgca aggcatcgga
     1981 aagagacgat ccctgattaa tatccttgac gatttggacg gttgcttccc tgatgctcgg
     2041 attttccgac tggaattcca ggatggacaa aattcttaat atcggaattc ccgcaaccag
     2101 catggagtgg aattgtttgg tgaataaaac caggtcggac ggtttgacct tgctttttcc
     2161 cagagccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tttggtgaat
     2281 aaaaccaggt cggacggttt gaccttgctt tttcccagag cctttaacca gccgcttcca
     2341 tacgcatctt gttcggaaat gtttatcgga ataagttggc gggaaacgag aatagagttt
     2401 gcgttttcca tggattcggc ttccagtttt ccggaaacct cttctcccat ctgattcact
     2461 gcctcgtagg tatattgaaa tgtgggcata ttctcctctt tgccttaagc catgaccgcg
     2521 gcgacagctt cttccagggt tgtaatgcct ttcatgacct tgttcgccgc gtctttctgt
     2581 aatgtcttga gtttaccggc agcaaccgcc gcgcgcgtaa tttcagggct tgaccgctga
     2641 ttaacaatca tgtcctgaat cgcttcatca atagccagaa cttcaaaaac acctgttctc
     2701 cccttataac cggtattgtt gcactgtgta cagcctttac cccgctggaa attggcgttt
     2761 tcagcttccc gagacgttat gccgaaatcg gctagcgctt tatccgacgg acggtaggac
     2821 tccctgcaat aaggacacac cgtgcgcacc aatcgttgcg caaaagaaac gatcagaacc
     2881 gaagcaatca gaaagggttc aattcccatg tcgatgaacc gggttatcac gccggccgaa
     2941 tcattcgtat gcacggtgct taaaagccga tgaccggttt gcgccgcctg aaccgctacg
     3001 gacgccgttt cggcatctct gatttcgccg accatgatta catcgggatc ctgacgaaga
     3061 atggaccgca atccgcttgc aaatgtcatt cccgctttgg tattcaattg cacctgccgg
     3121 atatcgttta cccggtattc gacaggatct tccagtgtta taatattaat atccggtttg
     3181 ttgatggcat ttaatatggc atacagactg gtgctcttgc cgctgcccgt cggtcccgtg
     3241 cttaaaatca tgccatacgg tttctgacac atgctttcta ttttagccat gtcctgggcg
     3301 tccatgccca ggctgtcaag ggtataaatg ccggcgctca tatccagaag acggaggacc
     3361 agattctccc cgtgaatggt gggaagggac gaaacccgga cgttgatttc cttgttttcc
     3421 gttttcatgg taaatctgcc gtcctgaggc acacgggaca cggtgatatc catattggct
     3481 aaaatcttga tccgggcgat cacggacaaa aaaaccgatt tgggaggaga cggcatttca
     3541 atgagcttgc cgtcaatgcg ataacgcagc tgaaccgtgt tgtgccgggg actgatgtgg
     3601 atgtcgctgg ccttgctgcg tacagcctga gcgaaaatcg aatttgtcag ccgcaccacc
     3661 agcgcttctc ccgcctgatc ctgcaaagac gtgatttgaa gggattcctg aagctcggta
     3721 tcgtcttccg ttttgctctc aattttcaga ccatccatga tgccgcccaa atcggattgc
     3781 acgccataca tgctactgat gagctgatta atctccattt cggaacagag cacgggttcc
     3841 acttccatgt tggttatttt ttcaatatga tcgagaactt tgatctccaa aggatcagcc
     3901 gttccaactt ccagcaaatt ccccttgatt ctcagaggag ccacctggtt tttctgcgca
     3961 taatcgaaag ggatgtaacg gatcaaatca aaatcaagag ggtattcgtt cacctgatac
     4021 ttcttgatgt tgagctgatc actcaacaaa tccaaaatct gtttttcctt gacaatgccc
     4081 tgccgtatta aatactgacc gaatttcaga ttggttttcc gttgttcact taatactttg
     4141 gcaagcattt cttcgcttaa aataccttgg ttgaccagta tctcacccaa tcgttttctg
     4201 gttttcatgc atcccccaat cagatcaata ttccggcgcg cggcggttga aaaagaccgt
     4261 gctcgcgtcc cattgagata aaattttcgc tttcgccgct aaagacggtg gcagtttgtt
     4321 tatagcctgc catgccgctt cctcattttt aaagcattgg ttaatcacaa cggcaaactc
     4381 aaagcctttc tttttattcc acaacgaaag aaacgccaat ggcggcagta tttgctcatg
     4441 aggatgattg cgaaaaagat tatacataat ttccagatct tgtccacttt ctaaagcgat
     4501 caggatgtcg cccggattca agggactgga gtcggcggga acggccggca aagttattat
     4561 cgttccttcc gcaacaacat ccttgttttt aatatgagga tttacggcaa aaatcttttc
     4621 catgaattga ggcgtgttgt cgccgtaaat attgtatgac acccaccaga gcgttcttct
     4681 ttttgccata gagatatttc ccagagcatc gggcattttc tcatccggga catccatagc
     4741 ctgaaccgtt gaatccccgg acggatgaac aacggacatt gccggtgcag attcattaat
     4801 gccggaaccg gttgaagcca accgcccctg tttttcttta cctgaatgga ggccctgcag
     4861 cgcaaccgca ccggctgaaa ttcccaaaac aatcaaaaat aaaagcccgg cgagtcccca
     4921 tcctaatttc cgggataggg gcgctttcat gttctcccgg ccacttcgca ccagaaacca
     4981 cccggctttc ttttcttcgc gaatgatcat ctttaaaaga acctgatgac acaaaaaaac
     5041 taccttgcgc ggatatcctc ctgtcgccag ataaacagca agaaagcctg agaagctgaa
     5101 cacaggggga tgtttttcaa aatggccggc caccttcaga cggtaattga tcatggcctt
     5161 catttgctta aaatttaacg gacgaagccg gtaaagaagg ttaacccggt ccagaagatt
     5221 ggctttcttt ttaagaatgt cgtaaaattc cttctgcgca aagataatga tctgtaaaag
     5281 tttgaattcg ttggtttcgt aattcagaaa ttcacgcagt gtttccaggc aatcgtcggg
     5341 aattttctgt ccttcatcga tcatgagaac aacgattttt ttttcgtcca cacccttatc
     5401 gaacaggcag tctttaattt tttcctttaa gtgccattcg ctttctgtct cgggttctga
     5461 atcgttgatg cccagcatgg acgcaaacgt ttgcagaaat tccaggttgc ttttaaatgc
     5521 gggatccagc aaaagatgcg gttcaatttc cgttttgccg gcatccttcg aagacggtga
     5581 gaagttctgg atgagtttac ggcataacgt tgttttgccc gtgccgatat ctccgatgat
     5641 tacattgatt cctcgacgca gacggacagc cagttccagc ttttgtaaac agtcgctgta
     5701 ctggggaagc tgaaaaagga actccggttc gggcgaattg gaaaacggct ctttttttaa
     5761 attgagaatg ttgaaatatt ccatttttaa cggccatgtt aacgtttcaa taccggccat
     5821 tgtttagcga cacggccgct ccgccggtat aatgccggtt ttgcccgttt tgtggatgat
     5881 gagttatctg atgctcaact acgtaccgaa taaccgatgt caactgttgc caaccggcaa
     5941 aatatgcggc gtgataaata taagcacttc ctccatattt tcacccttgc catccgattt
     6001 gaacgcccag cccagaacgg gaatatcctt taaccaaggc acgccgcttt gtgaatacgt
     6061 attggtctgc ttggtcaaac cggagatcac gatggtttca ccgtctttga caatcaattc
     6121 ggtttcagtc tgctttttga tgatgtaggg atctcccatg gaagatcttc gcgtcatatc
     6181 aacttcatct ttttttacta aaatcttcat ctttaaattc ttaccgtcaa tgacatgcgg
     6241 tgtaatcttc agttccatca caacatcttc gaatttggtg gtttgcgttg tcgtaccgcc
     6301 gctcgtttcc gttgtgacgt aaggaattct ttcaccattt tttgtagatg cttcctggtt
     6361 atccagcgtt gttatggacg ggctggataa aatatttagt ttgttatcct gctgcagggc
     6421 ctgtaactga acctccagca agttaccgtc gatcctgccg aacaacaacc ctaacgatcc
     6481 catgggagca ataacggatg acagatttgt tgtatccacg gggaaattca cacccaatcc
     6541 cgatcgttgc ggagccgttc ctgtgccgcc gataggcccg gccggagaaa caccgccacc
     6601 cgtgatgatg agatcttcct taccttgcgt acttctgttg tatccgcccc acatcacgcc
     6661 cagatctctg gccacgcctt tggtggtttc cacgatgttg gctttgatta aaatctgaga
     6721 ggtcggttta tccagttttt cgatgatcgg cagtatcttt gtcagatcat cgcgcatggc
     6781 ggaaatgatc aacgagttgc tatgatcatc gactttgatg gacccgcgcg gttttccatc
     6841 tttatctttt gtaagaagtt cctgggctgt ttccgccagt tttttggaat cggcgtaatc
     6901 cacactgaca acaaccggct ccaaaagcgg ctcgacccat tgaatatcac gcatctgaac
     6961 tttgcgttcc aattcctgtt taagatcatc cgccgtcata actctgatga tgtctccctc
     7021 ccagacataa gacaaaccgt aggtccgcaa caatccggta aatgcttcat cccaggcgac
     7081 ccctttaaaa tcaacactga tttccccttt cagctcgttt ttcaccagca ggttcttgtt
     7141 caccgatttg gccattgccc gcaacaccgc cttgagatcc gcctggcgca tggtcagatg
     7201 aatgggcgat gtgggaagtt tctttgcctc gtcactggcg cccaccattg gacttgcagg
     7261 atcttttttt gcaatgaggg ccgacgtgtc aatctttttg gggtgcgccg aaggggaatg
     7321 accttgagaa ttttcagcaa gcgtcgtcca cttctcgaaa aaagcgtcct ttttttcatt
     7381 tttgactccg ccggcacagc cccatacaaa catgagtgct gtgcaacaaa aaaccaaagt
     7441 catgattttt ttattcatta tagagtttct caaggcgata acctccaatt gacaaatttt
     7501 attcctgcag cgggatttcc agattacttc cgattcgttt atcaaatatt acaactttgg
     7561 aaggcatgat ttttttcaag acaaaccctt cctcctgcaa tgcttcacca actctatatt
     7621 cgatgccgtt aagcacggcg agtctgtttt tccccgtctc tatataaccc gaataaatga
     7681 tcggaacggc ggcaaccccg tctttgcctt tgctgtcttt ggccagccag gctctgtata
     7741 attctctttt caggaaagga ttcttatccc aatccgtctg cgttctctgg attacataat
     7801 gatcaaaatc ggaaatctta cttttattca actcaccggt tatcccgctg acgaccgctt
     7861 caaccttcac cgtttcctga tcaggcttca cttttttgcc tttctggcga tcagcaatca
     7921 gataaacata gacggcatac aagacaaaca gcgcggctat tgctaaaatg acaatttctc
     7981 ttttcttcaa tttggccata aatcccttta cctgcctatt gaacggctgt ccaaatcttc
     8041 attttaaaat ccatggcgcc ggcgttttgc tgaatgctga tttcttcgat tctttccaca
     8101 taaggaaggg cgcccagcgc gatcagcaat ttgcggaatc cagccagttc ccctttcagt
     8161 tccaggttat gtaaaaacga tgcggatgga ctggccgatg tattaatttc cggagcatac
     8221 gaaatgattt ttaatccgga tttctgcgct gccgcctgaa aatccttctg aatgattgta
     8281 atgtcctggc gaggcagcgc cgttttctgc gcatgcggca gaataagaac atcggtctgt
     8341 tccacattgc cggtcaatgt cttatagaca ggcccgagct ctctttgctc cttgatttgt
     8401 gacgttaatt tttcattttc attctgctgg ttggatactt tgaacgagta aggcaatatg
     8461 cccaccaaaa caataattaa cacaattccg ctgcagatca cggcgtagag aatacttttc
     8521 ttctgttgcg cattcagatt aaaatttttc atcatcacga acccagtttg gcacttaata
     8581 caaaatgaat gacgtctttc ttatcaaagt taacaatatt tttattttgc accgacaccg
     8641 tattgaaaag aggagaattt tccagtttta aaacgtattg tgtcaaatcg gattccagca
     8701 tatccttttt attaaaaatg atgccttcga gaacaagacc gttatgttcc gtcttttgac
     8761 cagccgctgc gggagaaccg gcggcttgcc gcaggcgcaa attaatgaga cgcacgtttt
     8821 ccggggtcgt atccgaaatc tctccgatca ccgcgacccc cagatatttt tgcgcatact
     8881 gccggaccgc gtctctctgt aatttgatcc tgttcacttc ctgaagaatc gtttcctgcg
     8941 acaccatcgg actgaaaagc gccagttcct tttccagggc cagattattc tttttcagcg
     9001 tactgaattt tgtcccctga taaaataacg taccgataca aaccatcagc gccgccagga
     9061 aggccatgaa aataatctta ttatttcgtt tcgatttaat gtcctgacgt ttttcttcaa
     9121 aagtaaatat ggcattgggc gtgtaactgt tatccgacag ggcaaacccc agagcgggag
     9181 aaatcagtat tctttcggaa gaacttaacg attgttcgga aataaccgat tttctattct
     9241 tgaaaggatc aaatatttca attcgggtat ccaattgatt gcccatgtaa tcaagcaaag
     9301 actggtcaaa attaatcggc gacaaaacat acatcttttc gactttttta ttgccgatcg
     9361 gcgacgtttt cagtgttaaa tcaatctgac gcgccaatct ctcccaaacg ggggcaatca
     9421 tattcagaat atcttccctg gcaaatttct gccggtcatc ggcctctttc gctcccccgg
     9481 attcgggcct catggtgcaa acgatcttct tggcttcagt ctgatccagc ttcaaatctc
     9541 cttttcttcc gctaaagccc gcaacaaccg cctctgccat actggaaaga ctgcctgttt
     9601 ttatccctct ggtcatgacc agattttctt tgtcataaac atcgatgcgc gaataattat
     9661 caccgataaa cagactggca aagatttctt ccgaggcagg aatccatttc gaccggaaaa
     9721 tattctgcat ggcgaaagga acgatggtaa ttccttnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnatcc atttcgaccg gaaaatattc tgcatggcga aaggaacgat
     9901 ggtaattcct ttaagattga tgccgagatt atcggcaaag gattctattc tcgcaatttc
     9961 cgatttttga gcggtataga acatgaccga atatttaggc gtcccctgct ctaccagttc
    10021 gccatggatt tcaaaatcaa aaatctgttt ttcttcatcg taaaacccct cttttttggc
    10081 cgtccagaaa atgacttttt gaatttgctt tttgggaaca cggggaattg tgaagaaata
    10141 aacgccgacc tgacttgtgg atatcttggt ccagatgtcg caatcggcga cattaccgca
    10201 aaaatttaag accgacgttt tgaccaactg attgaattcc ggagaaccta tgacgaaccg
    10261 gggatctatt gtgatgattt ttttatcgac taaaatcggc tgatgattgc tgtcttgagc
    10321 tgtttttacc aggcagataa attcacgccc gatatcgata ccgaccgtat acttcttttt
    10381 ggaaaggaat ttttcaagaa atcctgattg gaaaataccg gtttttttat ccggcgttaa
    10441 agaaattttc tgttttccga gcccgccgaa agacgcctcg tctttcccgc gtatcacatt
    10501 gagaagtttt tccgtagagt ttatgtcttt gctggtatta gacaaaataa tctcctggtc
    10561 gttgacgcat gcaccaatct aataaatcat ttttttaaat ttataatgta attccaaatt
    10621 cctgtaaatc attttattag attctttgtt ctcacaaaaa aaagaaagcc tcacagaaga
    10681 gcaattttta ttttgtatta atttcttgaa tgattatcag ttttttgcta aaagaacaat
    10741 acttttttta aaaaaactct gaatttacct gttcttatga ataagaagaa aaaaataata
    10801 atctcttgtc tcatccttct gctgatcggc gctctgttct acttcatgaa tatttattcc
    10861 accgttgctg cagatattaa attaagaggc tcttcatcgc cgcccttgac ggaaaaaaag
    10921 caaatcgcga aaccggcgtc atcagaccat tcttcctaca aaggtcagat accggattca
    10981 attgttgcct tttcgtcagg atacgcggtc gttgtcgata aagaacatca aaaattatat
    11041 gtcttttata aatcaggcaa atactccaag gtcttcgaaa cggtttgttc caccggtaaa
    11101 aaccccggtt ccaaaaggat tgcgggagat gcaaaaaccc ccaatggaat tttcttcgcc
    11161 accagaatcc tggaaaaccc gggaccttcg gaaacgtacg gttcactggc ctttacgctg
    11221 gattatccga ctctgtccga taaacgagca ggcaaagacg gcaataatat ctggattcac
    11281 ggaaccacca agccactgct tccgcaacaa tccaacggtt gtgtcgtgct aagtgatgaa
    11341 gatatgaaga aactggccaa cttcattcat ttgaacagaa cacccgtcat tatctccgaa
    11401 tccatcaatt gggttgatca ggattatctt tccgactcca gagatgaatt cgagaagata
    11461 ttatggtcgt ggaacaaagc atttgttgaa aaaaatttaa aggaaattga ttctttgtat
    11521 gctgaaggcg cccagatcag gggaaagaag cgagaagacc ttcactacaa aattaaaaat
    11581 ttggatttca ttaaaaaaca ttttgtttta cagcctcgcg acatcgccat cctgcaggaa
    11641 ggcgataatg ccgtcattat ttttgatcag atttttgacg tgaaggacaa caatactttt
    11701 cagggattct ataataaact ggttttagaa aaaataaacg aaaactggta tgttgttgat
    11761 gagtcctcga tatccgagcc tgcggacagg cgcctggcaa cagccaaaga cacgcaaaag
    11821 gatgccgtct cccccgaaaa aattgccgaa aaaaacatcc ggagcctggt cagaaaatgg
    11881 cgggcaagct gggaatcggg cagcatgaaa acatatcggg cctgttatgc gtctgatttt
    11941 caatcccgta acatgggact cgatgcctgg atagcgcata agattaaagt tcgccaaaaa
    12001 agcggcaaca ttaaaatcag cgtcaataat ttacaaattt ccatgagcgg caatacagcc
    12061 agggcaacat ttgtgcaaca gtacagttct tccattttaa gaaccaaagg ttcgaaaact
    12121 ctggaattga aaaaaagtgc cgacggatgg aaaatacata gggaaattat gtagagattg
    12181 ttgtccactt aaaccagcag cgttgtccat aaaaaaaccg gtgttttata gcaccggttt
    12241 tttaatttgg ttagaaaaac gacggcagtc atattttcag tatttcgtca tcctcctgtc
    12301 ctgcgatacc gtgttaacct tcgggacatc acgtttacgc aggcatgacg gtattgcttt
    12361 cttaatgtaa tgcctcacgc ggacgctgcc gaacccgccg ctttcatttc agcagactct
    12421 ttttttgcct tttccggctt ttgcatgacg atggcatcta ccggacattc caaagcgcag
    12481 aatccgcaac cgatgcagga tttggcgtcc ttcagatatg ggaaagcgtc cacgccttga
    12541 tttcgcggcc ggtccgacat agccaggcat ccagcgggac aggtcccgac acagatgccg
    12601 caagcgatac attttcccac gatgatttcc ggcttgggcc attcagatcg tttgatcatg
    12661 acgcacaaaa cgccatcggc atccaagacg cctccctgag gacaaaccct gccgcaggcg
    12721 ccgcattcga tgcatagcgc tgcgttgatc gaatgaattt tctttttttc accggtaatg
    12781 gcatccgcgg gacacactcg agcgcaggcg ccacagccat tgcatttttc cgtaattgta
    12841 tatgacatgc tgttacctct tatttgattc ccagtttctt caatgtttcc tctttgggtt
    12901 tgccgttggt cctgtcccaa ccaaactgtt cccagtaatc tgcggcaagt ttttccacat
    12961 cgacaaaccg tcccttattg ggaccttttt tctgaggaat cttcccctgt gcgcggctgc
    13021 tgataaagtt ttttctcggt tcgatgccgt gccggatgtt aaaattctgt ttgatcgtct
    13081 gaatccgttc gccgatggtc atgtattctt caggggtaaa cgtccagccg gtcgcggcat
    13141 ttagccagtc gaacgtggga atccttttcg ctccgataaa aaggccgaac atgcacatgc
    13201 ccgcgccatt ccccatgctc ataaatttac tgcatgcggc ggaagcttcg gccttctcct
    13261 tattgatctt atatttgctg cctttcatat agagaagatt gggcttgggc aatcctttga
    13321 cccgcttcca gagctgaaac atttcgtaat ataaatgcgc gccgatcgta tgccgacccg
    13381 gcgtgggttc cacactgtaa tggacattga agccgggatc gccgcgcccg tcatgcatgg
    13441 ccggttcctg acctcccgca tgaacagtgc actcttccgc gccccggccg atttttgccg
    13501 cggcaacttt ggatccatcg gccagaatat cgcctatgcc ttcccggtcg accatttttc
    13561 tgatgagggc gacaatcgct tccgtattgc cccatttcaa ttccaaaccg tccgtatctt
    13621 ccttcgtcag aatacctttt tcgtagcact caatggcgaa ggccaccgtt gcgcccgcgg
    13681 aaatggtgtc catgcccgcc cggttaagaa gttcgttcaa ataaaagagg ctgtccagat
    13741 cttcattcat gcacaaccct cccaacgcga ggatgctttc atattcgggt ttatgagtat
    13801 gggaatactt tcctttggtg gcgcaaatgc cgccgcatcc cagcggacac gaataacagt
    13861 gatattttac gatttccgcc tttttaatga tatccggatt gacggttttg gatttaccca
    13921 ggtggaaatc ccgactggaa ccttcccagt ttttcaaggg tgaatcgccc atctcaatgg
    13981 acatttgatt catgcctccc gtgccccatg ttcttaaaat ggttttgtac aggagaccat
    14041 cctgggcaaa catgaccgga gtcgccccca taaatgttcc cataagagcc aggcccacgc
    14101 ccggaagcag gccgagcggc atggggaagt tggtccactt gatacatttt tgactcagac
    14161 ttttcattga tttagggtct tgagcttgaa tccgttgcac accgtcaagg accactgctt
    14221 taagtttttt cacccccatg acagcgccta atcccgatcg ggcggcgatt ctgcctttgt
    14281 cggtacaaac accggaaatc aaagaaacct tttctccggc agggccgatg accgccacgc
    14341 ccgctctctc gccgtactgt ttcttgagtt tctcctccgt ttcgacggca tccattcccc
    14401 acaggtccga ggcatctttg agaaacgtct tgccgttttt tacatataaa taaaccggat
    14461 tggggctgat gcctttgaag aaaatggcgt catagccgca atgcttgatg gccggagaaa
    14521 acgtgccgcc gcaattcgcg tcgccccagc ctccggtcag aggcgacttg ccgaccagcg
    14581 tccatcgtcc tgaaaataaa ctgcctgtgc cggtcagcag accggaacag aagcccagaa
    14641 tattgtccgg ccccagaggg tcggcgccgg ccggtattcg gttgtaaagg atgtaggctc
    14701 cgagccccat gccggacaga tattgctggt acacactatc cggtattgtc tcttccgtca
    14761 tggttttgct ggacagatcc accatgagga tttttcccat ataagctttg ctcataaaaa
    14821 ctctctcttg taatgtttat ccttttttaa ttttattccg gtcaaggggc ttgccttccc
    14881 atgccgcttc cagcactaat aaattatctt catagtccgc atcttcggga ttataaatgg
    14941 ttgctccgtc aagcagggct gctttggcaa tttcaggaag aatctcccgt ggaaccatct
    15001 gcttcccatc gcggtctttg atctccgaga agaaccgcgc atgtcgcccg ccggtgatct
    15061 cgttgagatc ctgattcatc tgccggatca gggaaacaac cttatccgcc cgatccgcct
    15121 tggaggtctt gagatagacg tcctcgcccg ccatgggcag cagcagttcc ccgatggtat
    15181 ccccgcgctt atgcatattg tattcgagac catagggcaa taaaatggcc atgcagattc
    15241 catgaggaac gccacagaca gaaccggtgg catgaccgag attatggacc attccgacca
    15301 tggaattgga gaaagagatg ccggcaagac acgcgcttac cgccatggcc aggcgaccgt
    15361 ctttatccgc aggatttttg gtcaccgtgg gcagatagtg gtgaatcata ttgacggcag
    15421 ccagagagtg ggcgtcactc aacggatttt tctgaaggca gtaatatcct tcaacggcat
    15481 ggctcaaggc atccataccc gtcatggctg taatgaaagg cggcatggtc atggtcatcc
    15541 gggaatcgag aatggataca ctgggcaaca aaaaataact ggtaaaggcc atttttaaat
    15601 gcttttcatg atctttgatc acggcaacca gtgtgacttc cgatcccgtt cccgccgtgg
    15661 tcggtatggc aacgagagtc ttcaggcgcc gattcagggc cccggcgccg ctgaaggcca
    15721 tcagatcggt ggcattctcg gaaacgacga tgttgacccc tttggccgta tccatcacgg
    15781 aaccgccgcc gacggcgata atggcgtcac aggcgttatc ccggtaaaga ccggcgagct
    15841 ggttgaccac cttcaaatcg gagtccggag gaacattgtc ggcaatacct ccgatcgtaa
    15901 caccatcctt gagagcattg acaacgatat ccaccagacc ggcggcggca acgcctttat
    15961 cggtgatgat cagcggtttt tttgacccca tttcggcaag cagcgccggt atcttttcca
    16021 gcgcgtcgtg tcccgcaaca atttttacac ggcagcaaaa ttcataataa tccggtagta
    16081 tcatgggtta tatcctttcc gttcaggcct tggttgagcc gccgacacga tcgatgaccg
    16141 tgcgcaggta agtcccgtcc ggctcttgat tgatgacttc caatacatcg tccatcaggt
    16201 actgcgggat acccgcttcc ctgatggtct gcggcatttt gcccttcagc atactataaa
    16261 tctcatacgt aaatccatat acctccctca gagccgctcc tgtcctccct gcctccggca
    16321 tggcggcgta tttatcgtct ccgcccagca gaagcagtag gttggataca agagagttgc
    16381 cctttttcat ggtatcgtca agaagaggat aaaggcacat tccgatgatc acgcccggat
    16441 gaacatgaac cgtattctga aatacctgcc ccagtctgtg aagcacgttt tgatcggtat
    16501 tggaaataac acatcccgac atggccacgg cattggccac cgcaagggac tcttttttat
    16561 tacagcgttt cttgacggcc aaggcgaggt tctcccggat aaagcgcatt gcggtaaaag
    16621 cataggcgtc cataaaagga tttttatccg gagcaatgta cgcctccagg gtgcggccga
    16681 atgcggcaat acccgcttcg gcaacggttt tcccgtcttt tgcccgggtc agtcgcggat
    16741 caagcaccac gagatcgggc attaaatgaa ccgaggtaaa tgtctttccg cttaaaacgg
    16801 catatttcga cgtctccatt ccgttgacag caaacgtcgg aatgacgacc aggggtccca
    16861 ggggatcgcg aatggccgtt gccggcgaaa gctgacacgc atcggcaact ttttgtgaaa
    16921 ccgccaggtt cactattttc gccacatcaa caatgatccc gccgcccagg gcgatgatga
    16981 catcgtattg ccccttgaca aatatgtctc tgatttgttc gacaacgttc aaatcggccg
    17041 tctgtgttac atcatcaaaa agaccaagcg tcattcccga atcaccgaac gcgtttttca
    17101 acgttgaaat ggccctccag cccgccaact gacggctggt gatgatcagg ggcttgaaag
    17161 cattgaagcc ggccaactct acgggaagat gctccagagc tttgtttcct gaattcgtct
    17221 tgaacggaca gataaatgca aaaccgtttg taatattcat tttatattcc tttcattaaa
    17281 tccccaacaa agtgcgcgtg tagacgccga cccgtcctgc gagtttgcgg ccgaacgacc
    17341 accatgaggg ataacgtttc acagccagag aagcgatgat cttgggcaga agataaactt
    17401 ccacaatatc aagaatccgt atgaccgaca gggcataagg aacttcgccg tccgtcatca
    17461 gacgattacg ggttgtcgcc agagccgtgc tttcctgaaa agacaatatt aaaaatgcgg
    17521 catcaatact tttgataacg agacgaagat gaacaggccg gcatttgaga tcgccgccga
    17581 aatattttac cttgcccttt tgcttgccga ccaccatcca cggaccgttg ggcagacaac
    17641 ccagcgcaaa ggtgaattcg tcaggaagag catcaaattc tttttgaact tccttgtcga
    17701 cttttgccgc cgcctgaatg gctctgccgg taaaccacag gaatatagcc acgtaaattt
    17761 ttttcagaag tttccctccc gcatgaattt cccgcataat caaatcctcc cccttatctt
    17821 ctcttggaaa acagcatgat gctgcatatg ctttaacata tctagagatt attgaatcta
    17881 acattcatta caacgggcat gacactcatt gataagaaac caaagaatct aatgcgaatt
    17941 ataacggaca tgagtcttct tttttgctgt gatgcgattt agattagaac catttttttt
    18001 tacgatataa acaattttcg atttgaagtc aaagtaattt ttttggggtc gtcagacgca
    18061 ataagacggt ttttaacaac aacgcgcctg aatgcgcgcg ctgttgttaa aatttttaaa
    18121 atgatctatc catggcgttt ggcaggcttc aacgacagga catgagcttt gtaaatctgg
    18181 tcagcatgat cgggtgagtt aagagtcctg cgagtttaag ctcccggctt aaaaacagct
    18241 tcttcaacac ttcgaaaccg actttatcga aataataggg caggccgaat ttgacatcaa
    18301 tattggccag tgccagcagt gtatccgaat cggtgctgat ggatatcttg ggatctccca
    18361 ccttgccgct gtgcacggtc agactgcctt tttcaaagac caaggtcatg tcgacttccg
    18421 cgtcatgcgc ctgcatgtag atattgccgc ggagggcatt aaagtcgccc tgccgctgcg
    18481 gtttggcctc caaattgccc ttgatgatat cagcaatcat gacgggcaga ccggcatcct
    18541 ccacgccggg ggcaaaattg atctgagcca tagctcctcc ttcatatctt tgaatgaata
    18601 ttaatgaatc caaactacca tgcctgtttg atgatgccga gaacctcttc tgcttcaaaa
    18661 acaggtttgg gattataaac aatggcgcca tcggataacg tcccttcagc caactgaacc
    18721 agaccgtctt caggaacacc gacatccctc aaccgttgcg gtacgcccat tcttttggtt
    18781 aatgcggtta tggcttcggc caccgcttcg ctcgcctctt catcactcat ccccttagcg
    18841 taaatattca tggcctgggc gatcatgaca tatttatccg cgcaggtgtc catgttgtaa
    18901 cggacgacat gcggaagcat caggctgttg gctaatccgt gaggcacctg gaaaagccca
    18961 cccagcgtat gggcgatggc gtggacaaga ccgacctggg cattggtaaa ggcgattccg
    19021 gcgatggtag acgccattaa ctgctgacct cgcgcaaaca ggtctttgcc gttctctacg
    19081 cacaggggca gatattccat aatcaatcgg atggcgtgca gcgccatgcc gtccgcaacc
    19141 ggcgagcagg gtgtcgcatg aatggcctcc agtgcgtgac taaaagcgtc catgcctgtg
    19201 gtggccgtca gcatcggcgg caatcccgcg gtcatggtcg gatcgagaat cccgacattg
    19261 ggaatgatat tatcatcgca ataggggtgt ttctggtggt tgacctgatc cttgatcacc
    19321 gcaaagtacg tcacctcact gccggtgcct gccgttgtgg gaatgacaat atggggcgtt
    19381 tgcggacgcg taagacactg ctgcccctta taatcctcaa ttttaccgcc ttcctttaaa
    19441 acaatagcaa tccctttggt tgtatcaatc acgctgccgc cgccgacact gaccagaata
    19501 tcggctccct tgccccgggc aatttcggct gcatcattaa taatgcgcaa atcggaatcc
    19561 tgaatgcacc cgtcaaaagt tcccaccagt tttctgccca gcgctttttc cacccgttcg
    19621 gtcagtccgg cattcacgat gcccttatcc gtcaccaaaa aggccctgct gcatttcagc
    19681 gcatctgctt ccagtcccac ttcgttgatc gaattctccc cgtaaattaa tcttgtcgga
    19741 ttgcgataga tgaagttgag atccctgtcg tacgccatag ttcacctctc cttgaatgat
    19801 tgagtcatta atttatgagc atattgaaac gaaattataa aaatcagaca aacaccggat
    19861 atcggcgaaa taactccttc ctccatccgg tgtcactaag accgtttcta aacaagacca
    19921 aattcgggca gcaattcatt aacggtggcc cggaattttt ctgtttcttc aggcgtcagt
    19981 tcgtcctcat acttccctcc cacttcaccc actgtttcca caatgcgaac ggcgggcttg
    20041 accgccttga cgaccgtagg aaggtctccc ttgagtttga gtttcccctg catcattccc
    20101 ttgaccacat ccagttgttt tctcccgacg gcaaaccagc ggtcaatgga ggcagtgata
    20161 ataaaatctt tgggattttt cccgacttcc gatttgggaa tgtaacggat ggagcggcat
    20221 tcgccattgt gccagagatc catcctcaca aaaatatcat gatccagacc gaattgaggc
    20281 gccggcgtaa tatgaatgac gattgcattt tcccagttgg gcagggccac ttctttgtac
    20341 ttggcatcat tttgcatata cttttcccac tcttttaacc attcaggcaa aaaaatgata
    20401 aaaggttttg gctcattagc cagacgctct tccactctct tgttgtaagc ttcgtcccat
    20461 tccggtgttc catacgtgta cggcatatcc tttctccttt tccaaatttt attttattga
    20521 aaaccttcca ttccgatacg tttgtggaat ggatcagttg aacaatcccg ctatgcagca
    20581 ccatgaagtt tttgagccag gtcattgagg cctgctttct ccagaacatc tctgcgggga
    20641 aggccgctgg catcaagccc tcggagctca taaaattctg tgagcatttt ctcgatgtcg
    20701 ggaaccgacc cggcggtcat gccttcttcc agcggtttca tgacattcgg cggcagcgtg
    20761 tcgtcatccg cccgtgagcc gagcagattc gttatcccgc gcatcaaaag ccagatcttt
    20821 tctccggttt tgatcaaatc gtctatggaa taatcccagc ctgtcacgac atgaatcatg
    20881 tccgcaaaat ctttaacggc aatacagtca tacacaaaaa gacataaaca ggccgacgtg
    20941 ggcggcacgc cgatattctg agaaaggaca accatctccg cctttccatc gctggaggga
    21001 ccttcataat cggctttgaa cccggcatcc gcctgatccg tcattccctg ttcaatggta
    21061 tgactcatgt gttgaaggtg gcaggcgccg cgatatccca cagcgtattc cagtcccata
    21121 ccgtgaaacc cgcggggatc atgcgccggc agttccagtc ccttgaccgc caccacatac
    21181 tgttcggaac ccttgccgat aatttgcgcc gccttagcgg aaccttccgc caggacattt
    21241 ccgatgccct cgcgcttggc aatcttttcg accattttca gagcggattc gatatttccc
    21301 cagttcagtt cgattccatc ggtatcctcc gtggaaagaa taccgctatc aaagcactgc
    21361 atcgcaaagg caatcgtggc cccgcaggaa atggtatcta caccgtactg gttgcaggtc
    21421 tcattggcct taataatccc ctccagatta tcattttcca taaaggagcc aaatgtgcag
    21481 caggtctcgt actccggacc gggcccctct tcggtttgaa acggtccgcc ttccactctg
    21541 acgacgcgct tgcaggcaat cggacaggca tagcaggcat gcaccttggt caggtatttc
    21601 tcggtcatcg tcggtccgcc gatgttcacg gagccatcaa actcaccctt ggtccagtta
    21661 cggatcggca cgtctcccat catcattccc gggtccatcg aagccgctgt tcccatgctg
    21721 cgcaaaacct ggccgaactg gttttctttg attttctcaa gaaggttttc ccgaagggct
    21781 ttgtactctt ccggtaaagc cggcaaaacc ttgcctgtac cgctgaccac aacggcttta
    21841 agcattttag agcccatgac ggctcccata ccggttctgc cgatgtaatg cgctttgtca
    21901 ttggcaatgg ccgcaaagcg caccagattt tctccaccgg gtccgatcac cagcactttc
    21961 ggtttgcgct tgccgccatg ccgttccttt aatatgtcgg tcaccttata aaccggttct
    22021 ccccacagat ccgcagcgtc gcggatttcg acgttgtcat cttcgatcaa aagataaacc
    22081 ggtttttctg atttcccttc gataaaaatt ccatcgtatc cggcaaactt gagttccggt
    22141 ccgaacgtac cgccgcatgt gccgattccc cagaaaccgg tttgcggcga tcgggcacac
    22201 acggcaaaac gggatgtgcc cggaagcaat gttccgacaa gcggtccggt catgatgatt
    22261 aacggattgt cagcggagag ggcatcggag tcaggggtaa agcggttgag aaagaggtgc
    22321 gctgccagac cgcctcctcc caaataattt cttaaaagag attcatccag aggcgccttt
    22381 tgaattttta cctcggtgag attgatttcc agcagttttt ttgcatatcc tgattcacaa
    22441 tttgccatac cgtctcctga aaacaataat tcagtttcct gcttgtaatt atgggaactt
    22501 ttcctgaata ataagaacgt gtattgaaga agacattcaa cagccttgca gatgcttcaa
    22561 catcttcaat aatacagcgg tgttttcata aaatcccccc agatttatga actacactga
    22621 atgacaattt tttacagaat gtgccgcttc taacacaatt cgatttgtta cacaagatga
    22681 aattcaaaca gattaatttt tttcaacggt cctttatgct ttttctaaca tttgatgtat
    22741 cagccgtctt ttgctgtttc aaggctcgca acaaacctga tgaatcacgt tcccggcaag
    22801 aattagcttg acaacaccaa cattttttgt ttaattttct gcacttttca aggatatcaa
    22861 ccaaaattaa tttaaaaaaa ggaaggcaat aattatgaaa aagatattat ttactttgtt
    22921 aatggtattt ttatttttca cgccatcaat ttttgccgaa atggtccagg atatccccgc
    22981 gggaaccgcg gaagattact acaatcgggg aaataaatac agagaacagg gtaatctcga
    23041 ccaggctatc tacgattatt ccaaagcgat caaggcgaac tccaaatact ccaaggctta
    23101 ttacaaccgc gccaattcct atgtcaaaca gggcaaactc gctgaagcca ttgaagacta
    23161 cacaaaggct gtggaaatca atcccctgta cacggaagcc tactacaaca tgggcaatac
    23221 ttacgaaaag cagggcgacc tggctcaggc agtcgccagt tatacaaagg ctctggaaac
    23281 caatcccaga tactcagccg catactgcaa cagaggcaat gtgtatcagg cacagggtaa
    23341 tttccaacag gcgcttgacg attacaacaa agccgtcgag cttaacccga attttgccgg
    23401 cgtgtattcc aacagaggca atgtgtatca ggcacagggc aacaccgaca aggccattgc
    23461 cgactacaac aaagccatcg aactcagccc gaattttgcc ggattttacg ccaaccgggg
    23521 caatatttat cagagtcagg gaaatcttca gcaggccatc gccgactata ataccgccat
    23581 cgaaaaaaat cccgatgata cgtcctcctt ctacaacaga gggctggctt attacggcct
    23641 ggaagaatat gcaaaatcgc tagccgatta ctcaacggcg cttaatcaga atccggacaa
    23701 ggaagcctat gatgactttc ttaaatattt cccggaaaaa agaacatccg acaaccagga
    23761 tatccggggg gccattgttc aggtcttcga agataaatta aatttgccga agaaaatagc
    23821 cgctcctgtc gccgccgctc ccgccgatgc gataccgccg gcagccgcgg cagcagtcgt
    23881 tgccgacaca aaggtgtctt caacgcctga agaggatatc aagggttccg tcaacacgtg
    23941 gcttaacagc tggcaatcgg gagacatggc gacttaccgc agttgttacg atgaacgttt
    24001 tgaatccagg aaaatgaatc tggatgcctg gatcgattat aaaaataatg tacgcaacag
    24061 aagtaagaat ataagcattc aaattgatga tttgaaaata tccgtggacg gagatcgcgc
    24121 aaaggccgtc tttacgcaat cctacagttc ctcgctcctc aaagacaaag ggaccaaaac
    24181 ccttgagctc aaaaaagtgg gcgacaactg gaagatctat cgggaaatca tgtaaatcgg
    24241 acggtgggat gaagcgcgtt ttgacataaa agtccattgt aacataaaac caaatggcaa
    24301 atgcttttta aaaagcattt gccattttca ttgatccggc aacgtcagca atgaacataa
    24361 gcaaacagaa gatcgatcag gtcaaaggat ttctggacga acgcgaagcg gcctgtctgt
    24421 accgtctggc cttagaagcc ggcaagaagg gaccttgtct cgaaatcgga agctactgcg
    24481 gcaagtcttc cgtctatctg ggcacggcct gtaaagaaaa cggaaccgtt ttgttctccc
    24541 ttgatcacca caccggttca gaagaacagc agccgggcga agaatatttc gatcccgatt
    24601 tgctggacag ggaaagcgga aagatcgata cgtttaaatt cttccggaaa accattttcg
    24661 attttgacct ggacgatgtt gtcattcccg tagtcggccg ttcggcaacc atcggccgcg
    24721 tgtggaaaac gcccctgagc ctgatattca ttgacggcag ccacgcctat gactccgtgc
    24781 tcagcgatta cgaaatctgg ggaaaaaatc tgatgcccgg cggatatctt ttatttcatg
    24841 atatcttttc cgatccggcg aaaggcggac aggcgcctta ccgggtttat cagcaagcgg
    24901 tagattccgg acgttacacg gaaatgccca tgtgcgaatc actgggaatc ctgatccgca
    24961 agcaggaata atttcatttg gaccaggacc agcggcatcc ctgatcggac atttcagctt
    25021 tcaaatatcc gcgtccggca aaatcgtaaa gggtattgtc aatcacgccg gggtccgcct
    25081 gggtgatttt tccgattctg ttacaaagtt cccgcaacgc gttttctttc gagccccgac
    25141 agcattccgg ctcgatcaag ccgcattgct cgatgatgtc ctctttcaga agccgatagg
    25201 tcagtttcat ccgccagtgt ccttctacct gcattttatc ttcaacggag ccccggcctt
    25261 cgtgatatct ccggacgatt ttgttccagt cgtctgtcat ttctttttca tagccgtctc
    25321 cgaacgcctt catctcttcg ggattcagga aggatgttct gacaatcgcg tcgtttgcgt
    25381 cgtagcgcga ccagtcgtcc gtgaggatct gcagatcata gtcgccaatc ttctcccgca
    25441 ccgtcgttcc cggaaaaggc gccaggaaat ggtaaccgta aatcgctttg gtactcttgg
    25501 caaactgatc ggtttgatga agggtttcct tcgtctctcc cggcagtccg ataataaagg
    25561 aagcatgggc aatcatgccc gcctgctgac acattttcac cgccgaatag acctgctcca
    25621 gccggatccc tttcttgatc cgtttgagca tgtccggatt tcccgtttcc acgccgaaac
    25681 tgacgctgtc gcatccggct tcggccatca ggtcaaagac ctcctggctg accgtatcca
    25741 cgcgggcaaa agcgctccag ggaaatttca acgatcgttc cttgattccc gcacagattt
    25801 cccggaccct ttccttgtcg gtggtaaaga gatcatcggc aatattaatg cggtcgaaac
    25861 cgtaaccgat gatctgttcg atctcatcca gaaccaggcg ggagtttcgt ttgcgaactt
    25921 tggagccgac cattttgcgt cccagacaaa aaatgcacga ataaggacat ccgcgaccgg
    25981 tgatcagact gacgggaaat cccaatgcgc gataccgtga aatgggcaat aaatgccttg
    26041 ccggcagagg aatattatcg atgtccgtta cgaaatctct tttaggcgtg cggacgatct
    26101 catcgccttc ccgataggcg attcccctca cctcgcgcca tttttctttc tgcctgagca
    26161 cgggcgtgag ttcggcaatg gttgcctcgc cttcgccgat gacgatcaga tcgatttccg
    26221 gataatgctt cagcgtttcc tcgaccgtga acgatacgtg cggcccgccc atgatggtga
    26281 tgatctcatg attatactgt ttaacatcac gcagaatctt ctgcgcatcg taaaaattca
    26341 aggttaccga tgtcgccccg acggcatcgg gtgaaaacga cgccagttgg ttttcaattt
    26401 tttcacggga atatccggac acaatataat caaagatccg gacatccgca ccggcaccgg
    26461 cgaaggccgc ggccacatac gtcacgccca gcggcggcga cggcatttct tcaagcggat
    26521 atggcggagc aataacagct atacgcattt ttcaatctcc ctgacgagga aacagcacgg
    26581 accggaacca gccggccatg gtatttccct tcataattgc acgatcaatc acttcaaaat
    26641 cccgggccag cccggcggga tcggacagcc agtcttttct cgttctgatc ggcctgtccg
    26701 gatcaagata tttgatgacc ctggccggat tgcctgccgc gatggcgttg gcgggaatat
    26761 ccttcaccac caccgatccg gcgccgatga cggcgttatc gccgatcgtc acccctttgg
    26821 aaaccatggc actgtcgccg atccagacat tgttgccgat cgtcaccgcc tgcgtgttgc
    26881 cgaccggcat gctgcgatcg taaatatcat gccaatccgc atcgccgata ctgacacttt
    26941 gggccgtcat gcaaccgtca cccatggtaa ccgatgtcgc ggacatgatg cgtgtcccgg
    27001 gacagatcag gcagcattgg ccgatttcaa tcttgccctg ccccatccag accggccaca
    27061 ccgtaaaccg caccttccgg tcggaggtgg caatcaccgt cgtgtaatcc cccagggaaa
    27121 ccggcccgcc gaatatttcc acatgccacg gcttcataaa taccagcccg cgtccgaaat
    27181 attcgcattg cggagcgata taataacgcg cgtaccactt ctgaaacctg ttatagaagc
    27241 gtttaatgta atatggacgg tgatcttttc tcactgctga accttttgat attggattat
    27301 tgtagcagat attttttaaa aaccgggcgg ggacatcatc ggaaacagcc gctttgcaaa
    27361 aagcattttc acttgcttat cgacgcccag aattgatgat tgaaaagctt tcctgccggc
    27421 gtcatttcat tcagagcgtc cgaagccaga atgacggctg ccgccgtcca gcccgttttt
    27481 tcctccggcc agataacgcc atccggaaac gtcaccccca tccagaacga gccgtcggaa
    27541 aatcgtttat catgaagcca gctgaatatg gttttcgccc gggcaagatc gccgatcgac
    27601 gccagcgtca gaataaattc cgccgtctcg gccatggtca cccacggacg gtcgctgaca
    27661 cagcgcacgc cccagtcggg aacaacaaat ttttcccata aatgggcgat tcgtttctgc
    27721 gcctccgcac cggtgacggc tccgcataaa atcggataat accagtccat cgaatagcgc
    27781 gacttgatca tgttgaagag atccggcctg tttttaattg ccgcgcccag cgcgtccata
    27841 gccttgcgcc agcccgcgcg ttttttcccc agccggtcgg ctaccgccag cgcgcattta
    27901 atgctcatgt aaatggaact ggagccggtc agcaacgcca ttttatccac cgcgccgtgt
    27961 ttgtttctcg cccagtagat ttcgccgtcg ggcgcctgca gattgatcgc aaactgaatc
    28021 ccctgcgcaa tggtatgcca catctttttc aggaacttct catcgcgcgt caccagatag
    28081 tgatggaaga cgccaaccgc aacataggcg gcaaaattcg attctttgca ggaattgata
    28141 atttttccgt cctgaatttc cgaccaccaa ctgccgtctt caagctgagt cgacgccagc
    28201 cattcatagg ccctttccgc ctccttgtag aaaccggccg cggaaagccc catggcgctt
    28261 tctacatgat cccacggatc ggtctttccg ccggctgacc aggggatctc gccattctct
    28321 ttttgaagcg aaataataaa agaaccgatt ttttccacag agaccggagc gctcgcttca
    28381 ctgacggaga gttcaagttc cattccaatc aaccttttct aagataatag acaatgctct
    28441 tgcccatcaa cggattcagc aattcttcga gcatccgcac cggccgcggt tttttcataa
    28501 tgtcccactc cagaaatttc cgataacttc tgaccagcgg gtgatcttca tttttgaggc
    28561 ccaccaggca cttgagccac cagtatggtg aatgcaacgc atgcttgtaa ttgattttcc
    28621 agcacttgaa tccctggccc gtcagcatcc tgtgcagctg tttttttctg taaatccgga
    28681 tatggccgcc ttcttcatta tgataatcgc gggaaataaa ccagcagatt ctttcggcga
    28741 aataccgcgg cacactgacc acgagcgtcc cgttcggttt caggattcgg tccagctcct
    28801 ggatggcttt ttgatgatcg ggaatatgct ccagaacttc cgaacagatg atgcagtcga
    28861 aagacgcgtc ggcaaaaggc agccggttga tatcggcaat ggacaccaga taatccttcg
    28921 accgggcatc gggctgcgta tccatggatt tcgcggtttc ctgaagcgcc caggtgtttt
    28981 tatcgatgcc gaatattttc agctccggaa ttctcgccag atgacgcaga tgtctgccca
    29041 gtccgcaacc ggcatcgaga acgacgctgt cgggctttaa atcaagttta tcaatttcca
    29101 cggtaatcat gtattgcctc tcggtacact tccaccatgt cgccggcagc tttcgaccag
    29161 ttgaaaatgg catcgaccct tgcgataccg gcctgggcca ttttttttct ttgatcggga
    29221 tgattgaaca ggagcaggat ttctctggcc agagcatctg catccgcggg aggcacaata
    29281 attccggcat ctcccacaac ttccggcagg gcgccgccac tggtgctgac gagaggcacg
    29341 ccgcacgcca tcgcctcggc cgcgggcagt ccgaatcctt catataaaga cggaacaacg
    29401 gcaatcgtgg ctttggaata ataatcggca aattcttcat tggcgatgcg cccggtaaaa
    29461 tggacgatat cttcgatacc cagttcggcg acgagtttct taacgacacc gtttttcttc
    29521 ggctctccga ttaccgtcag tttcaccggc tgtttttttc tgatccggta aacggcttcc
    29581 agcagatagc gcagcccttt caaaggcgta tccgcgctgt tggtaacaat aatggagttt
    29641 tggggacgcg atccgttctg aaccggataa aaaaactctt tgttgatccc gttgggcacc
    29701 acgcgaaact tggacgacgg aatggaaaat tcctcagcaa tgtctatttt ggaacattcc
    29761 gaaaccgtca cgatatgcga aagctggcgg gcgacttctt tctgcatccg gatgaaggaa
    29821 taccattttt tgatcttatt aattttcctt ttgctggtcg ccgacttgaa ctcttcctgg
    29881 cggtcgacgg tgatgggatg atggatcgtc gctatcgtgg gaacgacgtt ctgcgctatt
    29941 ttaccgatcc cgtaagcaag acactggtta tcgtggacga tatcatactt cttccggtgt
    30001 cgttgcagat aattataagc ccggacgccg aaagtatacg gttcgggaaa ccggcccgta
    30061 cacatggtca gaaattcgta catattcagc ggattcatca gggcgcgcgc gttgcgcacc
    30121 cgaaagggat gttcgacatt ataaagatcc aggctgggaa gtctatgcag acgcaccggt
    30181 ggatccagat gcggataagg cggtcccgac aacacatcga cttcatgccc cagatcactg
    30241 agtgccttac tcaaatattt gatgtatact ccctgcccgc cacaattggg attgccccgg
    30301 taggtcagca gacaaatttt caacggctgt gatttggatt tacctttcat actgcaactt
    30361 ttttaccatc cttcgcgaat tttcaaacat agcggatact tctcttgcat tcagtccggc
    30421 gccgcttgca atgcgttgcg ctctttcccg gccggcaaaa tcagccggcg cgtgggcatc
    30481 attattgagc atcagcttcg ctttgcattt ccttgccgtc tgcgccacat gcccgttggt
    30541 gaaactgtga cctctccgcg ttgtgatttc gagataaatt cccctttcgg cggcaagcct
    30601 cgcttctttt tccgtcagga gtccgggatg cgccaggata tcaatgtctg ctttcagggc
    30661 agccagattt gttccggcag gcaccggctc cacaatcgtt tccccgtgta caaccaccac
    30721 cttcgccccc agttttctgg cttcattcgc caaaccggcg atctgagcgg gcgcaacatg
    30781 cgtgatttcg actccgggga aaacgttgat ctttcccgcc tggttaattt tgaaacaggc
    30841 cctgatgatc cggggaatga tgaaatcgat attggaatga tccgcgtgat cggtaatggc
    30901 cagaaactta taacccgcgg cccaggctcg ctgcgccagt tctgcgggta tcaactcgcc
    30961 atcgctgaaa atggaatgag tatgcagatc aatcacttaa aaaatcctga ttctaaatat
    31021 acgacagatt tagaatgctt aatctgatga ttactagcaa atttaggacc gggagtcaat
    31081 aaggatgtgc agaccgctaa gcattgaact acttggattt ttcctttaac agcagaaacg
    31141 ttgccgaaac gcggcagaca tttttgtctt tattgtcggt aaccagacag tctccgatca
    31201 tgaggctttt ccccttttta aaaacggttg ccgtcgccgt gagttccccg ccgctgacgc
    31261 cggccagaaa attgcttttc agttcgatgg tggttaagcc ttcctcagga gttaactgcg
    31321 tcatcatcgc atgagcgatc gtctcatccg ccagcgcaac catcaggcct ccctgcatgg
    31381 ctttggcccc ctgcagatac tccgggcgaa ccggcatttt aaaacaggcg taaccgtcct
    31441 tcagctccac cagcgccgca cccagaaaat ccagaaaagg attaatcccc ttttgaccgg
    31501 ctttcagttt ttccagataa acgtcgtaat tcacaatcat tcacgctttc cgagcaaatt
    31561 cactgcatcg gtttataaat cattttattt cattttccaa tagattaaaa aactattttt
    31621 cccgggggat cacatgaacg gacgtggctt tgaaggaggc caccaccgtg acgccgggag
    31681 caatgcccag atcctcgcac gacggaatgg tgatgtaagc gaccagggga aaaccgcaat
    31741 ccagaatgag cttattgaaa aatccctgac gaatgatttt agataccctg gcctcgaaac
    31801 tgttgcgcac gctgacttct tcctgcgtcc ggctggatat gataacattt tccggccgca
    31861 aacagcagaa tactttccgg ccgatggaac aatcgccgat cgcttcgatt tgcctgcctt
    31921 cgacatcgac aagcatattt cccgctgtac agttccgtac ccagccttcc agaatcgttt
    31981 ccgtgcccac aaagcttgcc acaaattccg aatcgggctg attgaagatc cgggcggtag
    32041 tgccgctttg cacgatattg ccggcgtgca tgacacagac atcctgcgcc agacgcagcg
    32101 tttcctcccg gtcgtgcagc gccagcacgg cggtcatctt cgttttctcc agaatctgtt
    32161 ttaaatcttc aatgatggtt tcccgcgacg gcggatccag cgaattgaac gcctcatcca
    32221 gcaggagcat ctgcggttcg atggccagcg cgcgcgccaa tgaaactctt ttggcctctc
    32281 cgccggagag cgccctggca taccgttttt ccaggtgact gatgccgaaa gcttccagag
    32341 atttttgaac cttgttgttg atctcgtcac ggctcatttt ccggaacttc aggcccagag
    32401 caatattgtc aaaaacattg ctgttcagaa gcagcggttc ctgaaataca accgatacgt
    32461 tccggcgcag gaaattcaga tccgcccggt tttccatcgc ccttccctga aaataaattt
    32521 ttccctgctg cggcttcaag agtcccgcca tcattaaaag cagcgtcgac ttgcccgcgc
    32581 cgttgggacc gatgagcgcc aggattcttc ccggctgtac gtgaaattcg ggaatagcca
    32641 ggacaacgac gccgccccgc ctcagaacaa tattttccac tttcatcatg acgttctgat
    32701 tcatcgcgga cgctgccttt gctgaatttg cgtgagcagg gcattaatca taaaggcgag
    32761 aatcagcaga ataatgccca gggcgatggc aatatcgaaa tttcccttgc cggtttccat
    32821 gaccgtggcc gtggtcagca cgcgcgaata gcctttgatg ttaccgccga ccataatgga
    32881 ggcgcccact tcggaaatca cacctccaaa tccggccatg accgccgcca gcagcggcag
    32941 tttcgcctct ttgatcaata tccacaccat ttgcaggcgg ctcgcaccca gagacaaaat
    33001 ctgcaggcgc agattcggcg gaagattttg aatagaagcc agcgagatgc ccatgacaat
    33061 gggcgtggca ataatcgcct gcgcgataat catggcatag ggcgtgtaaa gaatttccag
    33121 aaagccgaaa gggccgcttc gccagatcat catcgtcacg aaaagaccga caaccacggg
    33181 cggcagcccc atgcccgtat tgataaggct gataacgaat cgctttccga aaaaattatt
    33241 taaagcaacc gccgttccga tggaaacgcc gatgaaaaga ctgatgaacg tggctgtgcc
    33301 cgatatcttc aaagacagcc acgtgattcc caggacttca ggatcaaagg tgacgattaa
    33361 ctcaaaagct ttgatgatcc cttgaatgat taattccaca aaacacccgt taccttacga
    33421 cagaatcaaa cgcctaagtt cagccaaggc tttttgactt ggctgtttac aacataaaca
    33481 tcctttg
//
LOCUS       DAONQZ010000020        32835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1482_length_32835_cov_15.848444, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000020 DAONQZ010000000
VERSION     DAONQZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32835
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_32835_cov_15.848444"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLR13_05260"
     CDS             <1..332
                     /locus_tag="PLR13_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18777.1"
                     /translation="ASSATLSIGDSDGFTGYTGAGWLGANGIAISGNVAVISGTAVID
                     VGTNSASETRVNMTLPTVTLGAMNVTATMKLSNAMTMTGANVLGSLNLRGFSTQLTGG
                     SVQIYAH"
     gene            399..887
                     /locus_tag="PLR13_05265"
     CDS             399..887
                     /locus_tag="PLR13_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18778.1"
                     /translation="MKRLILLAIAVFWVMIPWVSSAKTVISDGELDAVIAEQGVSINF
                     TALTVGGTTTLTSASWGDSDGFGSGSTYPNSGYAGLNAVAIAGNLSVINGAMNVDVGT
                     SGTRTIVNMLLPTITLGTMSMNATMMMSQDVNLGSGNDLAYVTVQNLAATLNGTIKVY
                     AH"
     gene            918..1982
                     /locus_tag="PLR13_05270"
     CDS             918..1982
                     /locus_tag="PLR13_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18779.1"
                     /translation="MFKNNIARIIFCAVAVLLFLVPSLYSKMKPVSEEDLDLVTAESG
                     VTLGLDITASATAGNVGIYQSSSQAEGIFLSSLTMIEKLDDQVTGPEEPFRVLTNVNI
                     DVGTSGGRTWLNMSGLNFPDYTGNVSNSTSPYAAPNAVSLYFTNLQAKDSATTTYTLA
                     HNVYLYGMYIGKNVNLWTSGTDYVNFTPGDTFMRVSNHSTSTSSGIDIIQEMGLVMRY
                     LMIRPNTSDTRRWFVDGMILSRHTNANYNATADPVAWPNLSGSAKIGGLIPTYTYDTS
                     DYSITRNGYKSTTATIDVGRSSGGETAIRMNLPMAMSLNWKHLYIDQGGTGAKNLDTG
                     ATLLQNVSVYKAQLTIRDIY"
     gene            2063..2845
                     /locus_tag="PLR13_05275"
     CDS             2063..2845
                     /locus_tag="PLR13_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQC18780.1"
                     /translation="MGALVIAAIFAVIVSFTSSVYPLQEQSQSEINLTVPGPGMGNLR
                     VDVKPASELDKGHLIKQQYDFSCGSAALAILLNHYLGEKFTERQVIAGLMHYGNVGQI
                     QERKAFSLLDMKKFVTELGYQGEGYRAELDDLKTLTTPCILPISIMSYRHFVVFRGIH
                     DGHIFLADPWRGDISFTLDDFYDHWYEKVIFVVTPPEGGRTVSTLKLKPEDLVYITED
                     AARESMFGNQLNPALAAAEKEYKFMINPTADPKGMQYYRQKK"
     gene            complement(2899..3723)
                     /locus_tag="PLR13_05280"
     CDS             complement(2899..3723)
                     /locus_tag="PLR13_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQC18781.1"
                     /translation="MSRKVKAIILTGNGTNCEMEMAHACKMAGADTADIVHLSELLYG
                     ERRLDDYAFLNLPGGFLDGDDLGSAKAGANRFLHAGIRETEEMLFDQLIRFIRAGKLI
                     LGVCNGFQLMIKLGLLPALDGNYARQSATLTFNDSGRFEDRWVYLKTDPQTPCVYTQN
                     TDVLYLPVRHGEGKFIPQNETLLDRIEAAHLCALKYSTPEGHPTAQYPFNPNGSANAI
                     AGVCDESGRLFGLMPHPEAYLSRVNHPRWTREKLPEEGQGLVFFKNAVDFIRSRNF"
     gene            complement(3737..6709)
                     /locus_tag="PLR13_05285"
     CDS             complement(3737..6709)
                     /locus_tag="PLR13_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQC18782.1"
                     /translation="MISRIEVGFKKGIRDALGEKIRKRMIENFSLPVEHVATIDVYTI
                     AGDLTPGELNKAALGPLSDPVIQNFSINRPLAAGFDCLIEVGYRPGVTDNVGKTAREA
                     IKLILGRDNDIAVYTSRQYLICGQITEGDAEKIASGLLANDLIERYQIFFAGDFDTVR
                     GVPAYIPQVTGKAKPVVEEIRLSDARNDDLMTISRERVLALNLDEMKAIRDYFLDPRV
                     IAGRKKKGLGKNITDVEMECLAQTWSEHCKHKIFNSYIDYTDGKKQQAVDSLFKTYIK
                     GSTEKIRRAKGKKDFCLSVFVDNAGIIKFNDNYNLVFKVETHNSPSALDPYGGALTGI
                     VGVNRDPFGTGLGAKLIFNTDVFCFASPFHQKKLPPRILHPRRIYEGVREGVEHGGNK
                     SGIPTVNGSIVFDERFLGKPLVYCGTAGIMPARIQGKTTHQKDIRPGDLIVMTGGRIG
                     KDGIHGATFSSEELHAGSPVTAVQIGDPITQKKMTDFLLAARDRGLYRAITDNGAGGL
                     SSSVGEMATYSGGCELDLTHAPLKYQGLQPWEILISEAQERMTLAIDPKKIKPFLEMA
                     GKMDVEATVLGKFTASGKFHVLYGSQTVAWLDMHFLHAGVPQLRLHARWKQPRHHEPR
                     FSPPKHYGTELKNMLARLNICSKESVVRQYDHEVQGGSVVKPLVGAQNDGPSDAAVIR
                     PLLDSMEGVVVAHGICPRYSDIDTYHMTACAIDEAIRNAVAVGADIDHLAGLDNFCWC
                     DPVKSEKTPDGEYKLAQLVRANMAIYDYTTAFGVPCISGKDSMKNDYQIGRTKISIPP
                     TILYSVIGFIRDVRQAVTMDAKHPGDLVYIVGETKEELGGSEWFALKNGIGNTVPRVN
                     AANALKIYRALHRAMQKGVVASCHDCSDGGMAVAMAETALAGMLGMDIDLKHVPYTGR
                     KRNDYLLFSETASRFIVTVHPENQKLFDKIMTGNVTGLIGCVTEKPFLEIRGLEGKTM
                     IRETLKALKKAWQKTLNF"
     gene            6950..8239
                     /gene="rmuC"
                     /locus_tag="PLR13_05290"
     CDS             6950..8239
                     /gene="rmuC"
                     /locus_tag="PLR13_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQC18783.1"
                     /translation="MELILMIVVIILVLGGFALLLWRTGRRFADFSLLMGRIDNLRDF
                     QERTDRSVREEIARSRAEARTQAQQERTEITGSFKLFEDSIQNSLSDLTAATEKKIEA
                     VRLIIDTKLREIQEDNSRQLDRMRETVDEKLQTTLEKRLGESFRQVSERLEMVHQGLG
                     QMRELAADVGNLQKVLTNVKARGTWGEVQLGALLEEMLSPEQYLKNVRISETGGDVVE
                     FAIKLPGQSDLPDDCVLIPVDAKFPIEDYQRLLEAQERGDVAAADESVRQLETGIKKA
                     ARDIFQKYIAPPKTTDFGIMFLPSEGLYAEVIRRTALVGVLQREYHIVVTGPTTFAAL
                     LNSLQMGFRTLAIQKRSGEVWKVLGEVKTAFGRFGDTLDNVRKKLEQAANSVDDAQKK
                     TKTLSNKLKAVEASTDTRPTDDGITDDMPSHRASEHE"
     gene            8272..9672
                     /locus_tag="PLR13_05295"
     CDS             8272..9672
                     /locus_tag="PLR13_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC18784.1"
                     /translation="MNDITMERRHEDRRHDDRRRGERRKYERREDVRKMNQELIREQM
                     EKYRVLLKDIKDAYFEVDLLGNFTVVNSAMCSALGYGPEELIGSKYRKLVDKDGAKKL
                     QQAVERLHKNDEPVNLLDMEAAKKDGTKIIYEAMVSVIRETDGVVVGFRGVGRDVTRR
                     RQMEAELKRNQHDLIRKNQEIDQSRKDIQQALVSLEKAHEELKASQLKILQQEKMASI
                     GQLAAGVAHELNNPMSFISSNLSTLEKYINRLIQFIRVQSEAVASLQDAAVVRRVDEK
                     RAEVKLDYMVEDVRGLLKESLEGAQRVKKIVYELNSFSRMDEEEYKKADIHECIESAI
                     TIVWNELKYKSKLEKHYGDLPATMCYPRQMNQVFVNLLINAVNAIVDKGTITIKTWYE
                     DHAIRIDVSDTGAGIPRENLIRIFEPFFTTKAVGKGTGLGLSITYEIIQRHRGEITVA
                     SEEGRGTTFSIKIPVV"
     gene            9913..11055
                     /locus_tag="PLR13_05300"
     CDS             9913..11055
                     /locus_tag="PLR13_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQC18785.1"
                     /translation="MSKNIFRIIFVLIFSFLTAGVVLAAPPVKIGALFAVTGPASPLG
                     EPERNTAVMMVDEINKAGGIKGRKLELIVYDTQGDATKAVQAVNKLIKDDKVVAIIGP
                     STTGDSMAVIPVIEKSQVPLISCAAGIKITDPVKKWVFKTAQNDVLAVMRIYKNLQKQ
                     KISKVAILTVSDGFGSSGREQLRLQAKEFDISIISDETYGPKDTDMTVQLTKIRNSAA
                     QAVICWGTNPGPAIVARNARQLGIKIPLYMSHGVSSKKFIELAGEAAEGIMLPSGKVI
                     VADQLPDSDPQKKILLNYVSKYKERYKVEGDHFGGHAYDAVMLLKRAMEQGGFTPAAI
                     RDQLEKTKKFAGIGGTFSFSSRDHAGLTSDAFVLVRVEKGDWKLIN"
     gene            11265..12149
                     /locus_tag="PLR13_05305"
     CDS             11265..12149
                     /locus_tag="PLR13_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC18786.1"
                     /translation="MDNFQQLFQYVLTGLSNGAIYALIGFGFAVIYNSTGIINFAQGE
                     FVMLGGMLTVFLLTVLHVSLVPAIALAIVVSTLVGILFERFAIRPLKDAQPLSLIIIT
                     IGASIFIRGVAMLVWGKDTHALPAFSSHDPLSIAGATILPQHLWILGVTILIIAASRL
                     FFNHSIIGKAMRACSYNPRAAGLVGINVKSMVLLSFSLSAAIGSLAGIIIAPLTMTSF
                     DVGVMLGLKGFCAVIIGGMSSGMGTVLGGLLLGLLEALGAGFISASYKDAIAFIILLL
                     ILFIRPEGLFRKKETERF"
     gene            12199..13170
                     /locus_tag="PLR13_05310"
     CDS             12199..13170
                     /locus_tag="PLR13_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC18787.1"
                     /translation="MLKEKKHQIFIFVIFALAVLSVPFFLQGNYLLNVLVFVGINTML
                     AVGLNLLLGYAGQISLGHAAFFGMGAYISGIVTSRYPVEPFLIIFLAAVCVGLIAFVI
                     GFPILKLKGHYLAMATLGFGIIMYIIFNETVDWTGGPSGLSGIPSLKIGPVVFDNDQS
                     NYYLVWVFTLVVMMLSINLSNSRIGRALRAIHDSEVAARVMGVNARFLKVQIFTVSAV
                     ISAVAGSLYAHVMTFIAPASFGFNFSVELLTMIVIGGLGSVYGSFLGAVILTMLPEFL
                     RVFQDLDIVIYGLMLILMTMYMPGGLISGIEMAAEFFSSRLRKGKGE"
     gene            13167..13922
                     /locus_tag="PLR13_05315"
     CDS             13167..13922
                     /locus_tag="PLR13_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18788.1"
                     /translation="MMLRLSNITQIFGGLNALEDVSFSVKEGSITGIIGPNGAGKTTL
                     FNIVTGLYTQTGGDVFLGGENISFLETEELAGRGLVRTFQNVELFRKMTVLENVMVGL
                     HTKSSSGIFSCAFKLPRQIREEKAIREKSMVWLKYTGIAELAEMTAGNLPFGKGRLLE
                     ISRALAAGPKIILMDEPAAGLNSRETAELALLIRKIRQSGVTVVLVEHDMDLVMDVCD
                     AIVVLNLGKKLAEGTPREIQENPAVISAYLGEG"
     gene            14036..14779
                     /locus_tag="PLR13_05320"
     CDS             14036..14779
                     /locus_tag="PLR13_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC18789.1"
                     /translation="MLKIKNINTYYGQAQALKNVSLHLAEGEIVTLIGANGAGKTTLL
                     NSLSGVVPTRSGQIIFNDAPINNLPVHQIVRLGISQVPEGRQVFKPVSVEDNLDLGSY
                     LHHRILGGARDVKKNKEMVYDLFPILRERRKQLAGTLSGGEQQMLAIGRAFMTRPKLM
                     LLDEPSMGLAPMVVQEIFRVIASLSREQKTTVLLVEQNARAALKMAGRGYVLETGHMI
                     LEGTAAELLNNKDVQRAYLGRDKKEIWER"
     gene            14859..16160
                     /locus_tag="PLR13_05325"
     CDS             14859..16160
                     /locus_tag="PLR13_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HQC18790.1"
                     /translation="MNIWDQTHECMARDELEQLQLERLQAVVNRVYKNVTHYRKIFNE
                     TGIIAEDIRSLADLSRLPFTTRDDLQVNYPYGMFAVPLREVVRIHSSSGTTSKPIVMG
                     YTKNDIKIWSNLAARFMTAAGVTPDDVVQITFRYGLFTGAFGVHYGAEAIGASVIPMG
                     TGNTDKQIMIMQDYKSTVLVSTSSYAIALADRLDQLGIDPKSLSLKTGLFGGEPWSEK
                     MREEIENRLLLKATDNYVISELIGPGVAGECLCKKGMHIYEDAFIPEIIDPETGAVLP
                     AGAEGELVLTTLTREAFPLIRYRTGDVTSLDYTPCECGRTLVRMKKIMRRSDDMIIVR
                     GVNVFPAQIEDALFSVAQGETPYQIVVEREGAMDNLEVVVEVTDKIFSLELQKQRSFL
                     ETIKKRLASVTGINVVVRLVESKSIPRQQGRIQRVLDKRQI"
     gene            complement(16173..17237)
                     /gene="bioB"
                     /locus_tag="PLR13_05330"
     CDS             complement(16173..17237)
                     /gene="bioB"
                     /locus_tag="PLR13_05330"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQC18791.1"
                     /translation="MNFVDHLKNKIIRQSRFITPAEAMELFIEASGFCWSVLAAASEI
                     REHFKGKKIIFCGITNAKSGRCPEDCMFCAQSSHYKTTAHSYPLKKSAQIIADAEKAV
                     RNGAECFGIVTSGKQLKSKKERLEIIRAIQGVRETGLFPCASLGMIDKATAMELKAAG
                     LFRYHHNLETSRSFFKTVCTTHDYEESVKTLCAAKEAGLSVCSGALIGMGENISHRIE
                     LAQTLRKLDVDSVPLNILHPVKGTPLSHMRALPPMEILMTLAVFRFMLPDRDIRLCGG
                     KEKNLRQLLPLGIVAGANALMTGDYLTTAGQNSSLDHEMIADLGLTATRERITCTCNI
                     EKHSRPVARQKRRPVQKHPS"
     gene            complement(17234..17938)
                     /locus_tag="PLR13_05335"
     CDS             complement(17234..17938)
                     /locus_tag="PLR13_05335"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HQC18792.1"
                     /translation="MDKRLAYRIFFLFLFIALSAIIFIHYDLHHLFRDRDRLVNFILS
                     YPYDQFIFILVQIIQVVAAPIPGELTGIIGGYLYGPLWGTLYSTIGLTIGSWIAFMLA
                     RFFGEPLLENVVKKEVFDKFDHFMEHKGLLVSFLLFLIPGFPKDYLCYIMGVSRIPVG
                     TFIIISTAGRIFGTIMLSITGNVARNGQYILLTVILILGVTVFIAVYYYHDQLINILK
                     KEGAKLGHVKKERKSK"
     gene            18181..19539
                     /locus_tag="PLR13_05340"
     CDS             18181..19539
                     /locus_tag="PLR13_05340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC18793.1"
                     /translation="MAKIPQKLERKKSDIYKDAPIAKFGERKPDFSTMGRKMKNPHAR
                     FREVVCVEACRTPYGRSGGALKNFSAMELGAMAIKEVLRRTGGKVAPSDVDYIFMGQV
                     VPAGCGQIPGRQATILAGVPEFVPSITVNKVCSSGIKTVDLAFQMILLGRAEICIAGG
                     QESMSNCPFVLPDMRWGAKMALPNGRVVDSMVYDGLWDAFYNRHMAIHGSEVADEFGF
                     SRQEQDEWALNSQMNAVRAMQEGRLDDEVFPVEVRQGKAILIMDKDEGPRPETTLEGL
                     AKLPPVFNHTSTVTGRPGSVTAGNAPGVNDGGDVCLLMSRAKADELGLKPIFTLVDYA
                     EVSQPTKDIATVPGLAIKKILDQNDMTLDQMEVIEINEAFAAVALVSCRSILGMSRET
                     MFKKVNVNGGAIAYGHPIGATGARILMTLGYELRRRGGGWGVCGICSGHAQGDAMLIR
                     VD"
     gene            19761..20531
                     /locus_tag="PLR13_05345"
     CDS             19761..20531
                     /locus_tag="PLR13_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC18794.1"
                     /translation="MAGNPDMSIVVPVYGSDITLMPLYCRTVAAVSPISENFEFILID
                     DCGPGKPWEIIGAMAKKDSRVIGLKLAKNVGQHNAIKAGVDVAHGQWVVVMDCDLQDR
                     PEEIPRFWGKAQEGYNVVIGRRVERRDETIKRIFSYIFHGLLKWVTRQNSDAAQSNFG
                     IYSRQVIDKIKKLPEQSFYLPLLVRRAGFDVIPLDVEHDKRPYGKSGYTFSRRLSLAV
                     DILASSGDHPLKMFSLFRFFNASRGKPLYVVTDQIRMT"
     gene            20653..21804
                     /gene="rffA"
                     /locus_tag="PLR13_05350"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             20653..21804
                     /gene="rffA"
                     /locus_tag="PLR13_05350"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQC18795.1"
                     /translation="MKNKIEIPFNKPYIADKELIYIARAVMENNHTAGDGPFTKKCQI
                     WLEEKLHCRKALLTHSCTAALEISAMLADIQPGDEIIMPSYTFASTANAFVLRGGIPV
                     FIDIRPDTLNLDEALIEAAVTPRTRVILPVHYAGVPCEMDVIADIARRHQLFVIEDAA
                     HGILCSYKGRPLGSIGHFGACSFHETKSMISGEGGALLINDEKYVERAEIIREKGTNR
                     SQFVRKGLSQYTWVDVGSSYLPSDIIAAFLYAQMENVDQMISRRNAIYDVYWKQLEPL
                     EEKGLIRLPTQRDDCFRNGHIFYVITGSLRERIDLASFLKDRGISAFFHYVPLHSSPA
                     GKVYARTCGALEVTTAISNCLLRLPLYYAMTEEDVQAVVRAIMDFYRVR"
     gene            21870..23771
                     /locus_tag="PLR13_05355"
     CDS             21870..23771
                     /locus_tag="PLR13_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18796.1"
                     /translation="MDEREENGSNPVAAGNDAPQHPLRASSFFAFSEKLSASVNLRWL
                     MISLLPVLILLKYPVDRIDYDVWWQMAHGQYYLTHHTLKMDLSMFSWTPTDPTWIYNT
                     CLGSIAVYLFYHWAEGFGLWLFQWLIFSGVFLSFYLFLRSIHQRLDINSATVIAAVAM
                     ACSISCRYYKPELLSALLFCWTAHIFFHIKIRGNRYLFYLFPPIFAFWVNLHGAFIVG
                     LAFLGLAFAGEFLNRIFFPKKSLKTEDLMHFGLALILSGAATLLNPYGIDYLKSLVPT
                     IMNAVGVGDYSGPYDKFVLAYASLWPYLRKMGPELFGVGMTAWILTLMMMSIAFLVAY
                     DLMKHKSCDFALCIVSLALYWKGMETSRATYFFPIVFFFIFYYLLVSRLQMTKFGSKT
                     TVFSIMVFIFFFGMVSFFNIKYGADDKWFGRDLDGYVPVEEVEFLKKYKLPGPIFNDY
                     VIGGYLLWKLYPDYKVFIDPRGGLYRNQVFPDYMEFTIRHVSRDDIRRFREKYPFNIA
                     ILHYRQMALIFDFLKAGDDWRLLYFEKNAAILIHQSLLPLIEPIAGNISLSPIRFKHV
                     RNPEILTNVFNFYVRLDPRAGRYIYYVFKRNVSDGFFMKKEILTAMDSNIRLKEKAFQ
                     ERPDLFFHE"
     gene            23845..24618
                     /locus_tag="PLR13_05360"
     CDS             23845..24618
                     /locus_tag="PLR13_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC18797.1"
                     /translation="MSHGKDPCEVIMSSDAFKMFISEIDQERFGIKTARVVDMTADRL
                     PSVLDFCVSHAVKLLIARCSISDLGAAQSMEKQGFLLMDTLVYYTFDLLRRPVSSSDD
                     DVHFRPIRRGEENVVERVAIESFRGYFGHYHADPRLDRDKCDDVYVDWARKACVAKGS
                     DENFMVAEIQGRIVAFGVFRMNNPDEGELFLGGIHPDFRGQGIYQSFLHRAKAWCLSK
                     DARRMVISTQLNNIAVQKILVKFGFEITRGCYTFHKWFD"
     gene            24827..26560
                     /locus_tag="PLR13_05365"
     CDS             24827..26560
                     /locus_tag="PLR13_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18798.1"
                     /translation="MTAILVPLLIFLAYPLPRGDYDLWWQMALGKYYLTHHTLVIDHS
                     IFSWTPADSGWIYNTFLGSIILYLMYHCMGGFGLWLFQWLIFLGIFLSFYLFLRSTGQ
                     RLDVTALTVIAAIGITCSMVCSFYKPELFSALLFSWTVFIFFSVKVTRRKHLFYCYPV
                     LFAFWVNLHGAFILALIFLALIFTGEILNRIIFPRESFTSDELIHFGIAYFLSLAVTL
                     LNPYGITYHWSIYHGLTSEIYELNSKYISAYVSLWPYLKSVRSLFVLQGQVAWLFFLM
                     MMILGCIFLFDFIKKKSCDFALLFIFLATSWGSMRGMRGGYLLSFTFFFMFVYWIHRF
                     HWKTLTGKATLISLIIFFFLFINVANQNIRYGAYNRWFGAGLDESVPVAEVAFLKKYK
                     LEGPIFNDYLLGGYLIWALYPDYKVFIDPRLVPYHKQVAPDYWQLERMLPTPEDMRRF
                     NQRYPFKTAIIHYNRHFLIEDFLKAGWKLVYFEKNAAVLVHESMLAAVPPEVQWVDLG
                     PSRFRNVKDPVVLLNVFNIYVNVYPEASRVIYDIYRSNVSTWYEPREQHLRAMEEDMR
                     KMQSLQRKAKS"
     gene            complement(26601..28343)
                     /locus_tag="PLR13_05370"
     CDS             complement(26601..28343)
                     /locus_tag="PLR13_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQC18799.1"
                     /translation="MFITLAAVLFFGLHQAEGAFNESMAIDTKAISLANNVTADPPGT
                     ASIHYNPAGLSFMGDGSYISAGLIPAVLKMSGTFESDPNHQAFHDYQGNVIVDPLAGR
                     SSSTSTGRMFIPILNDTAPAVMGPIFGLSYRKPGSKWTFGYTTYAQYGGGWEYGDDDP
                     AMYGAKSVYLQHLIYAAPAISYRINNNLAVGASFGIGQTAMGLELKMRGPNEIVNITK
                     ILGDATQGMANPLFDVTVPLPLFAGGVGVYDNIGDLSLSLRDDFSPSYNLGVLWEPFD
                     WVALGLCYQSAVKSNVSGKFSLKYSEDWQRMVAWSGSTAVMQISSIIFDLPYTATDEQ
                     KGTVSTTLEFPQIVNVGVKLKPTKRLSLLGEVHWAEWSAVKENDLKFDQKVQFLQMAK
                     FMGYSGGSYNMKVAREFKDTLNWGVGVEYQLLDWLQLRAGYENRTSSTIDEYYDLMYA
                     LPSMRYFGAGMGIQWKDIDIDLALGYMISKSFKIRPGQSKNMNSYTLGDGLNTSYRGL
                     SYEQEVSVYLASLKATMPLHIVTDMIFGLLPFKFGSSKGSKVADLKEPVDSSANFMNN
                     MRYKNKYYFIEDSE"
     gene            complement(28555..29790)
                     /locus_tag="PLR13_05375"
     CDS             complement(28555..29790)
                     /locus_tag="PLR13_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQC18800.1"
                     /translation="MKRKFVIVLSLLFFLTFTPLAALPVMANEPLEGIQLALSGVSAD
                     DANAAADQEAVKAPKETTDVQETKAVEAPPVAEPQTPVTTQEGSAKSIEQELKDLKEE
                     IQKLRGENEARKKLEIPEEEKSQTIDDILSAAGRQYSLLKKGTVGLSYTFNYAYYSGD
                     AIDESTTVLRRVNHNLTNTLALEYAFFNNLTVSSNFPFVYKYNKVGTDESLDVTDLGD
                     ISIGFSWQPFKAGGRIPTTILSLSLSLPTGSSPFEINYEDELATGSGYYGIGAGVSFS
                     KVLDPLVAFGNLNYTYGIPESGLSQILYEDPSDPASKIYLEKVEPGSSIGVSMGFGYA
                     LSYQASLNMSAQFSYSFGSKYKSNTGTTETGSSLSSSFNVGTGWRITPSRSIYATVSM
                     GLTNNDPDVSLSLRLPYEF"
     gene            complement(30268..31383)
                     /locus_tag="PLR13_05380"
     CDS             complement(30268..31383)
                     /locus_tag="PLR13_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQC18801.1"
                     /translation="MILFLLIFLSLYGGINFYAFLKAKTLFHFSGKIEIIIIISMIFL
                     ILAPIIIKLAEKIHWETLARSIAYCGYLWMAFVFLFFVIHVSFDLIRPLLKIIFQTPG
                     GDSLKNILFFTALFLSLSFVVYGFFDAKNIQVKKLEIHTEKLPPGHETLRIVQISDVH
                     IGLIVRGKRLQNILEKVREAKPDILVSTGDLLDGELDNIASEAKRMMEIKPKFGKYAI
                     MGNHEYYAGIEKSLAFTKSAGFEILRDESKKIAGIHMIGLDDPTGRGYGVSEKNPSLS
                     NLFPVKKSKEFILLLKHQPTIREDKNFDLQLSGHTHGGQIFPFMFLTRIFFPKNSGYH
                     QLSDNKSVYISKGAGTWGPPVRFLAPPEITIIDVIKK"
     gene            31483..31558
                     /locus_tag="PLR13_05385"
     tRNA            31483..31558
                     /locus_tag="PLR13_05385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31516..31518,aa:Thr,seq:cgt)
     gene            complement(31750..>32835)
                     /locus_tag="PLR13_05390"
     CDS             complement(31750..>32835)
                     /locus_tag="PLR13_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18802.1"
                     /translation="TFPEQTLQYFEEADFAIRGEAEKGLTDLITHLSQPDQIAKDQIP
                     GLIWRESGAIRCNPPLFIENLDALGSPDWDLINPCHYPFQTSYFARSKIVAPLIMTRG
                     CPYHCTFCSCRAITGHKIRAHSVSYMIHQIKLLQSRFGIREVCFIDDNFLARKDLVME
                     LCEKILAENLDIRWSCFGIRLDLVDEQVLDLMEKSGCYLLTVGIESGSQRILDHMKKK
                     LTLNLIEEKLKLIKTKNGINVIGNFIIGYPAENEDDIRKTIKFAGKLPLYGANFFPFH
                     PTPGTEIYERLIAEKKLGPMNWQNMGQDMKPYVPEGISRQQFTWLFALAFINFYLRPK
                     VIRNVLRATKSLDKLKYIAYRIFKIIR"
BASE COUNT         8099 a         7565 c         8516 g         8655 t
ORIGIN      
        1 ttgcgtccag cgccacgctg tccatcggcg attccgacgg ctttaccggt tacaccggcg
       61 cgggctggct gggcgcaaac ggtatagcca tttcgggcaa tgtcgcagtc atcagcggca
      121 cggcggtgat tgacgtcggc accaacagtg cgtcggaaac gcgggtgaac atgaccctgc
      181 cgacggtcac cctgggcgcg atgaatgtca cggcgaccat gaaactcagc aacgcgatga
      241 ccatgaccgg cgcaaatgta ttgggcagtt tgaatttgag aggtttttcc acgcagttga
      301 cgggaggatc cgttcagatt tacgcccatt gattttagat ataaaaaaat tatatcgccg
      361 cttcaaaggt gtaaaaactg cttttaaaaa gggaaattat gaaacgattg atcctgttgg
      421 caatagcggt tttctgggtg atgattccct gggtgtcgtc ggcgaaaacc gttatttccg
      481 atggcgaact ggacgcggtg attgccgaac agggcgtcag cattaatttc accgccctga
      541 cggtcggcgg aacaacgact ttgacatccg catcgtgggg ggattctgac ggtttcggtt
      601 ccggatcgac gtatcccaac tccggatatg cggggcttaa cgcggttgcg attgccggca
      661 atctgagcgt gattaacggc gccatgaatg ttgatgtcgg caccagcggc acccggacga
      721 tcgtcaatat gctgctgccg accatcacgt tgggcacgat gagcatgaat gcgaccatga
      781 tgatgagtca ggatgtgaat ctgggcagcg gaaatgatct ggcctatgtg accgtgcaga
      841 atctggcggc cacgttaaac ggcacgataa aagtgtacgc tcattaatga ttttgaatgc
      901 agcgcggaga gaagttcatg tttaaaaata acatcgccag aataattttt tgcgccgtgg
      961 cagttttgct gtttctggtt ccatcgctgt attcaaaaat gaagccggta agcgaggagg
     1021 atctcgattt ggtgaccgcc gaaagcggcg tcaccctggg cctcgatatt acggccagtg
     1081 caacggccgg caatgtggga atttatcaga gttcctccca ggctgaaggg atttttcttt
     1141 cctccctgac gatgatagaa aaactcgacg atcaagtgac cggacctgaa gaaccatttc
     1201 gtgtgttgac taatgttaat attgatgtcg gcaccagcgg cggcagaacc tggctgaata
     1261 tgagcggcct gaattttccc gattatacgg gaaatgtttc taattcaacg tcaccctatg
     1321 ccgcacctaa tgcggtcagt ttatatttca caaacttaca ggccaaggac tctgccacca
     1381 ccacctatac tttagcgcac aacgtctatc tttacggaat gtatatcggc aagaacgtca
     1441 acctctggac cagcggaacc gattacgtca attttacgcc cggcgatacg tttatgaggg
     1501 tttccaacca ttccacgtca acgtcctcgg gaattgatat tattcaggaa atggggcttg
     1561 tgatgcggta tctgatgatc aggcccaaca cctccgatac gcgccgctgg tttgtcgacg
     1621 gcatgattct ttcccggcac acgaacgcaa actacaatgc aaccgccgat ccggtcgcct
     1681 ggcccaatct gtcgggcagc gccaaaatag gaggcctgat accgacatat acgtatgata
     1741 ccagcgacta ttcaattacg cgtaatggtt ataaatcaac tacggcaacc attgatgtcg
     1801 gcagaagcag cggcggggaa acggcgatcc gaatgaattt gcccatggcg atgtctctca
     1861 attggaaaca cctctacatt gatcagggcg ggacaggagc aaaaaatctg gatacggggg
     1921 ccacgctttt gcagaatgta agcgtttaca aagcgcaact gaccatcaga gatatttatt
     1981 aaaaactccg gttttatagt gttcgtcttt tacattacca atcagattta ttgagcgccg
     2041 gtttggaggt ttttttcatc ccgtgggagc attggtcatt gccgcaattt ttgcggtcat
     2101 tgtgtctttt acatcgagtg tctatcctct tcaggagcag tcccaatcgg aaatcaatct
     2161 gaccgttccc ggaccgggga tgggcaatct gcgcgttgat gtcaagcccg catccgagct
     2221 ggacaagggg catctgatca agcagcagta tgacttttcc tgcggctccg ccgccctggc
     2281 cattcttctg aatcattatc tcggcgaaaa attcacggag agacaggtga ttgccggcct
     2341 gatgcattac ggcaacgtcg gccagatcca ggaacgcaag gctttttccc tgctggatat
     2401 gaagaaattt gtcacggaac tcggttacca gggcgaaggc taccgggccg aactggatga
     2461 cctcaagacg ctgacaacgc cctgtattct gccgatatcc atcatgagtt atcgtcattt
     2521 tgtggtgttc cgcgggattc atgacggcca tatttttctg gccgatccct ggcggggcga
     2581 catcagcttt acgctggatg atttctatga ccactggtac gaaaaggtta ttttcgtggt
     2641 tacgcctccg gaaggcggca gaaccgtcag cacgttgaaa ttaaaaccgg aggatctggt
     2701 gtatatcacg gaagatgccg cccgcgaatc catgttcggc aaccaactca atccggccct
     2761 ggccgctgcg gaaaaagaat acaaattcat gattaatccg acagccgatc ccaaggggat
     2821 gcagtactac cggcagaaaa aataaacgtt ttcggcaaaa acctctgatt tttcgggagt
     2881 gatgtcgttt aggacgttct aaaaattcct gcttctgata aaatccaccg catttttgaa
     2941 aaaaaccagc ccctggcctt cttcgggcag tttttcccgt gtccagcggg ggtgattgac
     3001 gcggctcaga taggcctccg ggtgcggcat cagaccgaaa agccttcccg attcatcgca
     3061 cactccggca atggcattgg ccgatccgtt gggattgaac ggatattgcg ccgtcggatg
     3121 tccttccggc gtcgagtatt tcagggcgca cagatgcgcc gcttcaatcc ggtcaagcag
     3181 ggtctcgttt tgaggaatga attttccttc gccgtgacgg acgggaaggt aaaggacatc
     3241 cgtgttttgc gtataaacgc agggcgtttg cggatcggtt ttcagataga cccagcggtc
     3301 ttcgaagcgc cccgaatcgt tgaaggtcag cgtggcgctc tgacgcgcgt aattgccgtc
     3361 cagcgcgggc agcagtccca gtttgatcat cagctggaaa ccgttgcaga cgccaagaat
     3421 cagttttccc gcccggatga accggatgag ctgatcaaag agcatctcct cggtttcccg
     3481 gatgccggcg tgcagaaaac ggttcgcgcc cgctttggcc gatcccagat cgtcgccgtc
     3541 cagaaatccg cccggcaggt tcaggaaggc gtaatcgtcc agccgccgct cgccgtaaag
     3601 cagttcgctt aaatgaacga tatcggcggt gtcggcgccg gccattttgc aggcgtgcgc
     3661 catttccatt tcgcagttgg tgccgttgcc ggtcaggatg atggctttga ctttacgcga
     3721 cataaaaaat ccttctctaa aaattaagcg ttttctgcca cgcttttttc agagccttta
     3781 atgtttcccg gatcatcgtt tttccttcga ggcccctgat ctccagaaaa ggtttttctg
     3841 tgacgcagcc gatgaggcct gtgacgtttc cggtcatgat tttatcaaac agtttttgat
     3901 tttcgggatg cacggtaacg ataaagcggc tcgctgtttc ggaaaacagg aggtaatcgt
     3961 ttctttttct gcccgtatac gggacgtgtt tcaggtcaat gtccatgccg agcatgcccg
     4021 ccagggccgt ttccgccatc gccacagcca tgcctccgtc ggagcagtca tggcaggagg
     4081 caacaacgcc tttttgcatc gcccggtgca gggcgcggta tatcttcagg gcgttggccg
     4141 cattgacgcg cggcacggta ttgccgattc cgtttttcaa tgcaaaccat tccgacccgc
     4201 ccagctcctc tttggtttcg ccgacaatgt acaccaaatc gccgggatgc ttggcatcca
     4261 tggtgacggc ctggcggacg tcgcggatga atccgatgac ggagtagaga atcgtcggcg
     4321 gaatggaaat ctttgtgcgg ccgatctgat agtcgttttt catgctgtcc ttgccggaga
     4381 tgcagggaac gccaaacgcg gtggtgtaat cgtaaatggc catgttggcg cggaccagct
     4441 gggccagctt gtattcgccg tcgggtgtct tttcggattt caccgggtcg caccagcaga
     4501 aattatccag ccccgccaga tgatcgatgt cggcgccgac cgccacggca ttgcggatgg
     4561 cttcgtcgat ggcgcaggcg gtcatgtggt acgtgtcgat gtcgctgtag cgcggacaga
     4621 tcccgtgcgc aacgacaacg ccctccatgg aatccagcag cggccggatg acggcggcat
     4681 ccgacgggcc gtcattctgc gccccgacca gcggcttgac gacggagccg ccctgcactt
     4741 catgatcgta ctggcgcacc accgattcct tggagcagat gttcaggcgg gccagcatgt
     4801 tttttaattc cgttccgtaa tgcttcggcg gtgaaaagcg cggttcgtga tgccggggtt
     4861 gtttccagcg ggcatgaagg cgcagttgcg gaacgccggc atgcaaaaag tgcatgtcca
     4921 gccaggcaac ggtttgggag ccgtacaaaa cgtgaaattt tcctgaagcg gtgaattttc
     4981 ccagaacggt tgcttccacg tccatttttc cggccatctc cagaaacggt tttatttttt
     5041 tcgggtcgat ggccagtgtc atccgttcct gcgcttcgga gatgagaatt tcccagggct
     5101 gcaatccctg atatttcagg ggagcatgcg tgagatccag ttcgcagccg ccggaatagg
     5161 tggccatttc acccacggag gaggaaagac cgccggcgcc gttgtcggta atggcccggt
     5221 acaggccgcg gtcgcgcgcc gcgagcagaa aatcggtcat cttcttctgg gtgatggggt
     5281 cgccgatctg cacggccgtc accggggagc ctgcgtgcag ttcttcggac gaaaaggtgg
     5341 cgccgtggat gccgtccttg ccgatgcgtc cgccggtcat gacaatcaga tcgcccggcc
     5401 ggatgtcttt ctgatgggtg gttttgccct ggatgcgcgc gggcatgatg ccggccgttc
     5461 cgcagtacac caggggtttg cccaggaatc gctcgtcaaa aacaatgctg ccgttgacgg
     5521 tcgggatgcc gcttttgttc ccgccgtgtt ccaccccctc gcgcacccct tcgtaaatgc
     5581 ggcgcggatg caggatgcgc ggcggcagtt tcttttgatg aaacggcgac gcaaagcaga
     5641 atacatcggt attgaatatt agtttcgcgc ccagtcccgt cccgaacgga tcgcggttca
     5701 cgccgacgat gccggtgagc gctccgccgt acgggtcaag tgcggaagga gaattatgcg
     5761 tttccacctt gaaaacaagg ttgtaattgt cgttgaattt tatgatgccg gcattgtcca
     5821 cgaaaacgga aaggcagaaa tccttttttc ctttggcccg ccggattttt tccgtggatc
     5881 ccttgatgta ggttttaaaa agagagtcga cggcctgttg cttcttgccg tccgtataat
     5941 caatgtaact gttgaatatt ttgtgtttgc agtgttccga ccaggtctgg gcgaggcatt
     6001 ccatttccac atccgtgatg tttttcccga ggcctttttt cttccgtccg gcaatgactc
     6061 gtggatcaag gaagtagtcg cgaatggcct tcatttcgtc aaggttcagc gccagaacgc
     6121 gttctctgct gatggtcatg agatcgtcgt ttcgggcatc ggaaagacgg atttcttcaa
     6181 cgaccggttt cgctttgccg gtcacctgcg ggatataggc cgggacgccg cggaccgtgt
     6241 cgaagtctcc ggcgaaaaaa atctgatacc gctcgattaa gtcgttggcc agaaggccgg
     6301 acgcgatttt ttccgcatcg ccttcggtga tctggccgca aatcaggtac tggcgggagg
     6361 tgtagacggc gatgtcgttg tccctgccca ggatgagttt aatggcttca cgggcggttt
     6421 tccccacgtt gtcggtgacg ccggggcgat acccgacttc aatcagacaa tcgaaaccgg
     6481 cagccagcgg gcggttgatg gaaaagttct ggatcaccgg gtcggaaagc ggtcccaaag
     6541 cggctttgtt cagctctccc ggcgtcagat cgccggcgat ggtgtaaacg tcgatggtgg
     6601 ccacatgttc cacgggaagg gagaaattct caatcattcg ctttctgatt ttttcaccca
     6661 gagcatcgcg gatgcctttt ttgaaaccga cttcaattct gctgatcatg ttcaaccttg
     6721 acgttttaat tttttctgat gaacggacga tgcgttatac ccaaaagcgg caaattatgc
     6781 aacttacaaa taggcttttg cgtcaaaatt catcctaacg tcatgaaatg gccttttatg
     6841 gataaaatct ggtcatttcg cttgacaggg gactttttta atcgttaaat gctttcgctg
     6901 tttccgtagt attctttcct aaaaaactgc tgactgatag cggagaaaaa tggaattaat
     6961 cctgatgata gtcgttatca tccttgtgct gggcggtttt gcgcttttgt tgtggcgtac
     7021 gggacgccgt tttgccgatt tttcattatt gatgggcaga atcgacaacc tgcgggattt
     7081 tcaggagaga accgaccgtt ccgtgcgcga agaaatcgcg cgatcgcggg cagaagcccg
     7141 gacccaggcg cagcaggaac gaacggagat aacggggtct ttcaaattat ttgaagattc
     7201 catccagaac agcctgtcgg acctgacagc cgccacggaa aagaaaatag aggcggtccg
     7261 gctgatcatc gataccaagc ttcgggaaat tcaggaagac aatagccgcc agctggaccg
     7321 gatgcgggaa accgtggatg aaaaactgca aaccacgctg gaaaagcgcc tgggagagtc
     7381 cttccggcag gtgtcggaac ggctggaaat ggttcaccag gggctgggac agatgcgcga
     7441 actggccgcc gatgtcggca atttgcagaa ggttttaacc aacgtcaagg cgcgcggcac
     7501 atggggagaa gtacaactgg gcgcgctttt ggaggagatg ctttcccccg aacagtatct
     7561 gaaaaatgtc cggatcagcg aaaccggcgg cgatgttgtg gagtttgcca tcaagcttcc
     7621 cggtcaaagc gaccttcccg acgattgcgt actgattccg gtcgatgcaa aatttccgat
     7681 cgaggattac cagcgcctgc tggaggctca ggagcgtggg gatgttgccg cggcggacga
     7741 atctgtccgc cagctcgaaa caggcattaa aaaagccgcc agagatattt ttcaaaaata
     7801 cattgctccg ccgaaaacca cggatttcgg cattatgttt cttccgtcgg aaggacttta
     7861 cgccgaagtc atacgccgga cggctctggt gggcgttttg cagcgcgaat atcatatcgt
     7921 ggtgaccggt cccacaacgt ttgccgcgct tttgaacagt ctgcagatgg gtttccgcac
     7981 gctcgccatt cagaaacggt ccggcgaagt ctggaaggtt ctgggagagg tcaaaacggc
     8041 tttcggaaga ttcggcgata cgctggataa tgtgcgcaag aaactggagc aggccgccaa
     8101 ttcggtcgat gatgcgcaga agaaaacaaa aacactttcc aataaactga aagccgtgga
     8161 agcgtcgacg gacacacggc cgacggacga cgggataacg gatgatatgc cgtcgcaccg
     8221 ggcaagcgaa catgaatagt caacgcaata cctctggagg ggatcgaaac catgaatgat
     8281 ataacgatgg aacgccgtca tgaagatcgc cggcatgacg accgtcgacg cggtgagcgc
     8341 cggaaatatg agcgccgcga agacgtgcgc aaaatgaatc aggaacttat tcgcgagcaa
     8401 atggaaaaat accgcgtgct gctcaaggat atcaaggatg cgtattttga ggtcgatctt
     8461 ctcggtaatt tcaccgtcgt caacagcgcg atgtgcagtg ctctgggata cggaccggag
     8521 gaattgatcg gctcaaaata ccgtaaactg gtggacaagg acggtgcaaa aaaacttcag
     8581 caggccgtgg aacggcttca caaaaacgac gaaccggtca atctgctgga catggaggcg
     8641 gccaaaaaag acggaacgaa aattatttac gaggcgatgg tttccgtgat cagagaaacg
     8701 gacggcgtgg tcgtgggatt cagaggggtc ggccgggatg tgacccgccg ccggcagatg
     8761 gaagctgaat tgaaaagaaa tcagcacgat ctgatcagaa agaatcagga aatcgatcag
     8821 agccggaaag acattcaaca ggcgttggtc agccttgaaa aggctcatga ggaattgaag
     8881 gcgtcgcagt taaaaatcct tcagcaggag aagatggcat cgatcgggca gcttgccgcc
     8941 ggcgtagcgc atgaactgaa caatcccatg tcctttattt ccagcaatct cagtacgctg
     9001 gagaaataca tcaaccgtct gattcagttt atccgcgtac agtccgaggc cgtcgcatcg
     9061 cttcaggatg ccgccgttgt gcgccgtgtc gatgagaaac gcgcagaggt taaactcgat
     9121 tacatggtcg aagatgtccg gggactgctg aaagaatctc tggagggggc gcaacgggtc
     9181 aaaaaaattg tctatgaact caactccttt tcccgcatgg atgaagagga gtataagaag
     9241 gcggatattc atgagtgcat cgagagcgcc atcaccattg tctggaatga attgaaatac
     9301 aaatcaaaac tcgaaaagca ttacggcgac ctgccggcca ccatgtgtta tccccggcag
     9361 atgaatcagg tgttcgtaaa tctcctgatc aatgccgtca acgcgattgt cgataaagga
     9421 accattacga ttaagacgtg gtacgaagac catgctatcc gtattgatgt ttcggatacg
     9481 ggtgccggca taccgcgtga aaatctgatc cggatcttcg aacctttttt taccaccaaa
     9541 gcagtgggga aggggaccgg tctgggactg agtattacct atgaaattat tcagcggcat
     9601 aggggagaga taacggttgc cagtgaggaa ggcaggggca caacgttttc gataaaaatt
     9661 cctgttgtgt gaagaccgga agatttttta aaaatattcc ggtctgccgg cggcggggat
     9721 gaatttccgt ttgacacaga aagtgttctg tggtttacaa ctcgtcaaaa atattaagct
     9781 attggaaaga cagaccgggc gaatttttaa tcaaaggggt tagtcgtttc accggcgtat
     9841 tgccggcatt ggagctttta agttttttta ttcgtttgaa tggttgtgga ataaacttaa
     9901 gtggaggtgt gtatgtcgaa gaatattttc agaattattt ttgtattgat tttcagcttt
     9961 ttaacggcgg gggtggtgtt ggcggctcct cccgtaaaaa tcggcgcgct gtttgccgta
    10021 accggtccgg catcgccgct gggcgagccg gaacgcaata cggcggtgat gatggttgat
    10081 gaaatcaaca aagccggcgg aatcaagggc cgcaagctgg aactgattgt ctatgatacg
    10141 cagggagatg ccaccaaagc cgtccaggcg gttaataaac tgatcaagga tgacaaagtc
    10201 gtggcaatca tcggtccgag cacgacaggc gacagcatgg ccgttattcc ggttattgaa
    10261 aaatctcagg ttccgctgat ctcctgcgcg gcgggcatta aaatcaccga tccggtaaaa
    10321 aaatgggttt tcaaaacagc gcagaacgat gttctggcgg tcatgcggat ttataaaaat
    10381 ttgcaaaagc agaaaatttc caaagtggct attctcacgg tctccgacgg attcgggtct
    10441 tccggacgcg aacagctgag attgcaggcg aaagaattcg atatcagcat tatttccgat
    10501 gaaacgtacg gtccgaaaga cacggatatg accgtgcaat tgacaaaaat acgcaacagc
    10561 gcggcgcagg ccgtcatctg ctgggggacg aaccccgggc cggccattgt tgcccgaaac
    10621 gccagacagc tggggatcaa gattcccctg tacatgagtc acggtgtttc ttccaagaaa
    10681 ttcattgaac tggccggaga agcagcggaa ggcattatgc tgccttcagg caaggtgatt
    10741 gttgctgatc agttgccgga ttcggatccg cagaaaaaaa tcctgttgaa ctacgtgagc
    10801 aaatacaagg agcgctataa ggtggaaggc gatcatttcg gcggccacgc atatgacgca
    10861 gtcatgctgc tgaagagagc catggaacag ggcggattca ctcccgcggc cattcgcgac
    10921 cagttggaga aaaccaaaaa atttgccggt atcggcggaa cgttcagttt ctcttcccgg
    10981 gatcatgcgg gtctgaccag cgatgccttt gttctggtca gggtcgaaaa gggcgactgg
    11041 aaattaatca attaaccgat ccggcattcg gattcatttt tttataaacg gcgcggaacc
    11101 aaaaccgcgc cgttttctgt tcgtccagtg actcaaacat aaattgatac catttctaaa
    11161 ttaaaaatga tattgaatcc acgattgcct gcgttttaca gacggcggaa tcggcggcca
    11221 cccggcctgt gtgacgcgta ttcgatgcag agacggaatg ggatatggac aattttcaac
    11281 agctatttca atatgttctg accggtttat ccaacggtgc catttatgcc ctgatcgggt
    11341 tcggatttgc cgtgatttat aattccaccg gaatcatcaa tttcgcccaa ggggaattcg
    11401 taatgctcgg cggcatgctg actgttttcc tgctgaccgt tttgcatgtg tcgctcgtgc
    11461 cggccatcgc actggccatt gtcgtgtcga cccttgtcgg aattttattc gaacgtttcg
    11521 ccatccggcc tttaaaagac gctcaaccgc tgagtctgat cattatcacg atcggcgcca
    11581 gtatttttat ccgcggagtg gcgatgctgg tctggggaaa agatacacac gccctgcccg
    11641 ctttttccag tcacgatcct ctctcaatcg ccggcgccac gattctgcct caacatctct
    11701 ggatccttgg cgtgacgatt ttgatcatcg cggccagcag gctatttttt aatcacagca
    11761 tcatcggcaa agccatgcgc gcttgttcct ataatccgcg cgcggccggg cttgtcggca
    11821 ttaatgtgaa gagcatggtg ctgctttctt tttccctgag cgccgccatc ggttctctgg
    11881 ccggtattat tatcgcgccc ctgacgatga cgtccttcga tgtcggggtc atgctcggcc
    11941 tgaagggatt ttgcgcggtc attatcggcg gcatgagcag cggcatggga acggtgctgg
    12001 gcggtctttt gctggggctg ctggaggctc tgggcgccgg ttttatttcc gccagttaca
    12061 aagatgccat cgcgtttatc attctgctgt tgattctatt tattcgtccg gagggattgt
    12121 tcagaaaaaa ggaaacggag cggttttagg cgccggccgc ggataaaccg tttacggatt
    12181 ggtgcattat atttgatcat gctaaaagaa aaaaaacatc agatattcat cttcgtcatt
    12241 ttcgcactgg ccgtgttgtc ggtcccgttt ttcctgcagg ggaattatct tttaaacgtg
    12301 ctcgtgtttg tcggcatcaa caccatgctg gcggtgggac tgaatctgct cttgggatat
    12361 gccgggcaga tttctctggg gcacgccgcc tttttcggca tgggcgctta catctccggc
    12421 atcgtgacgt cccggtaccc tgtcgagccg tttctgatca tttttctggc ggcggtctgt
    12481 gtcggcctga ttgcttttgt gatcggattt cccattttga aactcaaggg gcattatctg
    12541 gccatggcca ccctgggctt cggcattatt atgtacatta ttttcaatga aaccgtggac
    12601 tggacaggag gaccttcggg cttgtccggc attcccagtt tgaaaatcgg tccggttgtt
    12661 ttcgataatg atcagagcaa ttattacctg gtgtgggttt ttacgctggt tgtgatgatg
    12721 ctctcgatta atttgtccaa ctcgaggatc ggccgcgcac ttcgcgcgat ccatgattcg
    12781 gaagtggccg cacgcgtgat gggcgtcaat gccaggttcc tgaaggtgca gatttttacg
    12841 gtttctgccg tgatttcggc cgttgccggc agtctttacg ctcacgtcat gacgtttata
    12901 gcgcccgcgt cgttcggttt taatttttct gtggagctgc tgacgatgat tgttatcggt
    12961 ggtttgggca gcgtttacgg gtcgtttctg ggggcggtca tcttgaccat gctgccggaa
    13021 ttcctgcgcg ttttccagga tctggacatc gttatttacg gattgatgct gattctgatg
    13081 acgatgtata tgcccggcgg tctgatcagc ggaattgaga tggcggcaga atttttttcg
    13141 tcccgcctga gaaaaggcaa aggtgaatga tgcttcgact cagcaacatc acgcagattt
    13201 tcggcggact gaacgccttg gaggatgtgt cgttttcggt caaggaagga tccattaccg
    13261 gcattatcgg tcccaacggg gccgggaaaa ccacgctttt caatatcgtg accggccttt
    13321 acacccagac cggaggggat gttttcctcg gcggagaaaa tatttctttt ctggaaaccg
    13381 aagaacttgc cggccggggg ctggtgcgca catttcaaaa tgtggaactg ttccggaaaa
    13441 tgaccgttct ggaaaacgtg atggtcggtt tacacacgaa gagttcaagc ggcattttct
    13501 cgtgcgcttt taaactgccg cgtcagatca gggaagaaaa agctatccgg gaaaaaagca
    13561 tggtatggct taaatatacc ggcattgccg agctggcgga aatgacggcc ggaaatctgc
    13621 ctttcggcaa gggacggctg ctggaaatct cccgggcgct ggctgccggg ccgaagatca
    13681 ttctgatgga tgaaccggcg gcaggattga acagcaggga gaccgccgaa ctggcgttgc
    13741 ttattcgcaa aataagacaa tccggcgtaa cggtcgttct ggtagagcac gatatggacc
    13801 tggtgatgga tgtctgtgat gctattgtgg tcttgaacct gggtaaaaaa ctggctgagg
    13861 ggacaccgcg ggaaattcag gaaaaccccg cagtcatctc cgcgtatctc ggtgaaggat
    13921 aatgaggttt ttaaaaaatt gtccgatgct gcaacgtgca acaaaaatgc gtatcaaccc
    13981 ttgtctcgac cgcgtctaat cgccggtatt tttagggagc cgttgggtaa acaccatgtt
    14041 aaagataaag aatatcaata catattacgg acaggcgcag gcgttgaaga acgtctcgct
    14101 gcatcttgcc gagggcgaaa ttgtcacact gatcggcgcc aacggggccg gcaaaaccac
    14161 cctcctgaat tctttatcag gggttgtgcc cacgagaagc ggtcagatta tcttcaacga
    14221 cgcgccgatc aataatcttc cggttcatca gattgtgcgg cttggtattt ctcaggttcc
    14281 ggaaggccgt caggtgttta agcctgtctc cgtggaagat aatctggatc tggggtccta
    14341 tctgcatcac aggatactcg gaggcgcccg tgatgttaaa aaaaataaag agatggttta
    14401 tgatctgttc cccatcctcc gggagagacg aaaacagctg gccggaacct tgtccggcgg
    14461 agagcagcag atgctggcta tcggccgcgc tttcatgacc cggccgaagc tgatgctgct
    14521 ggatgaacct tccatggggc tggcgccgat ggtggtgcag gagattttcc gggtgatcgc
    14581 ctcgttgtcg agagaacaga agaccaccgt tcttctggtg gaacaaaatg cgcgcgcggc
    14641 gctgaaaatg gccggccgcg gttatgtttt ggaaacagga cacatgatcc tggaagggac
    14701 agctgctgaa ctgctcaaca acaaagatgt tcagcgagcg tatctcggca gggataaaaa
    14761 agaaatatgg gaaaggtaag cagccgaagt ttggagaaga acggtccgtc aacgtcatca
    14821 atatttttca acaactttcc ttttacggag gttattttat gaatatatgg gatcagactc
    14881 acgagtgtat ggcgcgcgat gagctcgagc agctgcagct ggaaagactt caggccgtgg
    14941 tcaaccgggt ttacaaaaat gtcacgcact atagaaaaat atttaacgag acaggcatta
    15001 tcgcggaaga tatccgttcc ctggccgatt tgagccggct gccgtttacc accagagacg
    15061 atttacaggt caattatccc tatgggatgt ttgccgttcc gctcagagag gtggtgcgca
    15121 ttcattcttc ctccggcacc accagcaagc cgatcgtgat ggggtacacg aaaaacgata
    15181 tcaagatatg gtcgaatctg gcggcgcgtt ttatgacggc tgccggtgtg actcccgatg
    15241 atgtggtgca gattactttc cggtacggac ttttcaccgg cgcgttcggc gtgcattacg
    15301 gggccgaggc gatcggtgcc tcggtcatcc cgatgggaac cggaaatacg gacaagcaga
    15361 ttatgatcat gcaggattat aaatcaacgg ttctggtcag cacatccagt tatgccattg
    15421 ccctggcgga tcgtcttgat caactgggta tcgatcccaa atccctttcc ctgaagacgg
    15481 gactcttcgg tggcgagccc tggtcggaga agatgcgcga agaaattgaa aacaggcttt
    15541 tgttgaaagc gaccgacaac tacgttattt ccgagctgat cggtcccggc gttgccggtg
    15601 aatgtttgtg caaaaaaggc atgcatattt atgaagatgc ctttattccg gagattattg
    15661 atcctgaaac gggggcggta ttgcctgcgg gcgccgaggg cgaactggtg ctcacgacac
    15721 tgacccgtga ggcttttcca ttgatccgct accgcacggg agatgtgacc tcgttggatt
    15781 atacgccgtg cgaatgcggc cggacgctgg tccgcatgaa aaaaatcatg cgccgttcag
    15841 acgatatgat cattgtccgc ggcgtcaatg tctttccggc gcagatcgaa gatgccctgt
    15901 tcagcgtggc tcagggggaa acaccctatc agattgttgt ggaaagggaa ggggccatgg
    15961 acaatctgga agttgtggtg gaggtgacgg ataaaatatt ttctctggag ttgcagaaac
    16021 aaagatcgtt tctggaaacg attaaaaaac gtctggcttc ggtcacgggg attaatgtcg
    16081 ttgtccgcct ggtggaatca aaaagcattc cgagacagca aggccggatc caacgggtgc
    16141 tggataaacg acagatataa acgacaccgg gattatgaag ggtgcttttg aaccggtcgt
    16201 cttttttgac gggccaccgg ccggctgtgc ttttcgatgt tgcatgtgca ggtaatccgt
    16261 tcccgcgtcg cagtaagccc cagatcagca atcatctcgt gatccagaga actgttttgc
    16321 cccgccgttg tcagataatc accggtcatc agagcattgg cgcccgccac aatacccagg
    16381 ggaagcaatt gacgcagatt tttttccttt ccgccgcaga gccggatgtc cctgtccgga
    16441 agcatgaaac ggaaaacggc gagggtcatc aggatttcca tgggcggtaa cgctcgcata
    16501 tgactcaaag gtgtgccctt gaccggatga aggatattca gaggaacgga gtcgacatca
    16561 agttttctca aggtttgagc cagttcaatg cgatgagaaa tgttttcccc catgccgatc
    16621 agagcgcccg aacaaaccga caaaccggct tctttagccg cacagagtgt tttgacgctt
    16681 tcctcgtaat catgcgtggt gcagacggtt ttaaaaaaac tccgcgatgt ctccaggtta
    16741 tgatgataac ggaacagccc ggccgctttg agctccatag ccgttgcttt gtcgatcatt
    16801 cccagtgagg cacaggggaa aagacctgtt tcgcggacgc cctgaatggc tctgatgatt
    16861 tccagccttt cttttttcga ctttaactgt ttgccgctgg ttacgatgcc gaaacattcc
    16921 gctccgtttc tgaccgcctt ttcagcatcg gcaatgattt gtgcggattt tttcagagga
    16981 tacgagtgtg ccgtcgtttt gtaatgcgag gattgcgcgc agaacatgca gtcttccggg
    17041 catcgtccgg atttggcgtt ggtgattccg cagaaaataa ttttctttcc cttgaaatgt
    17101 tcccggattt cggaagcagc ggccagaacc gaccaacaga agccggacgc ttcaatgaac
    17161 agctccatag cctcggccgg ggtgatgaaa cgcgattgcc ggataatttt gttttttaag
    17221 tgatcgacga aattcatttt gactttctct ctttttttac atgaccgagt ttggcccctt
    17281 cttttttcag tatgttaatg agctggtcat gataataata aaccgcaata aaaaccgtta
    17341 cacccagaat cagaataacc gtcagaagga tgtactgccc gttgcgggcg acgttgccgg
    17401 taatcgaaag catgatggtg ccgaaaatgc ggccggctgt ggagatgata atgaacgttc
    17461 cgaccggtat gcggctgact cccatgatgt agcatagata atccttggga aagccgggaa
    17521 tgagaaaaag caggaaggag accagcagac ccttgtgttc cataaagtga tcaaatttgt
    17581 caaaaacctc tttcttaacc acattttcca gaagcggttc cccaaaaaac cgggccagca
    17641 tgaaggcaat ccatgaccca attgttagac cgatggttga ataaagagtt ccccacagcg
    17701 gtccgtaaag ataaccgccg atgatgccgg tcagttcgcc aggaatcggg gcggcaacga
    17761 cctgaatgat ctgcacaaga ataaaaataa actggtcgta aggataggaa agaataaaat
    17821 tgaccagcct gtcccggtct cggaatagat ggtgtaaatc ataatgaata aagatgatgg
    17881 ccgacaaggc aatgaacaaa aataaaaaga aaattctgta agccaggcgt ttgtccatgg
    17941 atggttttga tccttacaat tgcttgattt gaaccggttg gcgatttttt atagcattat
    18001 tctgctttcg tcaatcccat tattggatcc atccggcggt ttttttgttc atcttccgga
    18061 aaaatccgtt aaacccgttg attttaaaaa tgccctgtgc aaaaacaagt gaaaaatttt
    18121 tcatcctggt ttatagggtg ttttatgaaa agcgaaacac aaaaagggga ggaaaattgt
    18181 atggctaaaa tcccgcagaa actggaaaga aaaaaatcgg atatctataa agacgcgccg
    18241 atagcgaaat tcggagagag aaaaccggac ttttcaacaa tgggccggaa aatgaagaat
    18301 cctcatgccc ggtttcgaga ggtggtctgt gtcgaggcct gtcgaacgcc ttacggccgt
    18361 tcgggcggtg cgttaaagaa tttttcggct atggagctcg gcgccatggc cattaaagaa
    18421 gtcttgcggc gcaccggggg caaagtggcc ccgtcggatg ttgactatat ttttatggga
    18481 caggtagttc ccgccggctg cggccagata ccgggtcgac aagctacgat tcttgcgggt
    18541 gtgccggaat ttgttccgtc tataacggtc aacaaggtct gttcgtccgg catcaaaaca
    18601 gttgatctgg cttttcagat gattcttttg gggcgcgcgg agatctgtat tgcaggcggg
    18661 caggaaagca tgagcaactg tccttttgtt ctgccggaca tgcgctgggg agcgaaaatg
    18721 gctctgccca acggacgggt tgtcgattca atggtttacg acggcctctg ggatgcgttc
    18781 tataaccggc atatggccat tcatggctcg gaagttgcgg atgaattcgg attttccaga
    18841 caggagcagg atgaatgggc gcttaattcc cagatgaacg cggtacgtgc catgcaggaa
    18901 ggcagactgg atgacgaagt ttttccggtt gaagtccggc agggcaaagc aatattgatc
    18961 atggacaaag acgaggggcc gcgtccggaa acgacgcttg aggggctggc gaaacttcct
    19021 cccgtattta atcatacgag taccgttacc ggccggcccg gcagtgtaac cgccgggaat
    19081 gctcccggcg tcaatgacgg cggagatgtc tgtctgttaa tgagcagggc gaaagctgat
    19141 gagctgggat tgaagcctat ttttaccctc gtggattacg ctgaagtttc ccagccgacc
    19201 aaggacatcg ccactgttcc ggggttggcc atcaaaaaga ttctggatca gaatgacatg
    19261 acgcttgatc agatggaggt cattgaaatt aatgaagcct ttgccgcagt cgcactggta
    19321 agctgccgtt cgatactggg catgtccagg gaaacaatgt ttaaaaaagt caacgtcaac
    19381 ggcggggcga ttgcttacgg tcatcccatc ggagcgaccg gtgctcggat tttaatgacg
    19441 ctgggttacg aattgagaag acgaggcggc ggctggggcg tctgcgggat ttgttccggg
    19501 cacgcacagg gcgacgccat gctgatcagg gtggactgaa atctgttccg aaaggctctt
    19561 gagggaaatc tccgggatta tgaaaaacca aatcattttt gattgctttt tgctaaagcg
    19621 gctttttctt acagtccggt tttgaaaaaa aatcaacata caaaagagtt gaaaatgatg
    19681 gtaagttatg taaaggttca tgtccaaaac cttaaaatat cagtaaattt tgttggtcaa
    19741 tcatttgcaa ggggttcttg atggctggaa atccggatat gtccattgtt gtgccggtct
    19801 atggaagcga tatcaccctg atgcctcttt attgtcggac tgttgctgcg gtcagcccga
    19861 tttcggaaaa tttcgaattt attctgatcg atgactgcgg accgggaaaa ccatgggaga
    19921 tcatcggcgc catggcgaag aaggattccc gggtaatcgg tctgaaactt gcgaagaacg
    19981 ttggccagca caatgcgatt aaagccgggg tggatgtggc gcacggacaa tgggtagtcg
    20041 tcatggattg tgatctgcag gaccgtccgg aagagattcc ccgcttctgg ggaaaagcgc
    20101 aggaaggata taatgttgtg atcggccgtc gggtggaaag gcgcgatgaa accatcaagc
    20161 gcatattttc atatatcttc cacggtcttt tgaaatgggt aacccgccag aatagtgatg
    20221 cggcgcagtc caatttcggc atttattccc gacaggttat tgataaaata aaaaaattgc
    20281 ctgaacaatc tttctatctg ccgttgctgg tcagacgggc gggcttcgac gtgatacccc
    20341 ttgacgtgga acacgataaa cggccttatg gaaaatcggg ctatacattc agccgaagat
    20401 tatctctggc tgtggatatt cttgcttcct ccggcgatca tccgttgaaa atgttttcgt
    20461 tgtttcgatt tttcaatgcc tccagaggga aacctctcta cgtggtgaca gatcagatcc
    20521 ggatgacgta agaaagcaat acccttattg agtttcgacc ctgcccggtc ggggttatgc
    20581 cttcggacag gagcctgatc gtctttagaa aaatcacggc tgaaaatgga cacggtgtgg
    20641 agaaaatgaa ctgtgaagaa taaaatcgag attcctttta ataagccgta tatcgctgac
    20701 aaggaactta tttatattgc ccgggcggtg atggaaaaca accatactgc cggagacggt
    20761 ccctttacaa aaaaatgtca gatctggctg gaagaaaaac ttcattgccg caaagcgctc
    20821 ctgacgcatt cgtgtacagc ggctctggaa atttcggcaa tgctggcgga tattcagcct
    20881 ggcgatgaaa ttatcatgcc gtcctatact tttgcatcga cagccaatgc ttttgttttg
    20941 cggggcggaa tccctgtatt tatcgatatc cggcccgaca cgctcaatct tgatgaggcg
    21001 ctgattgagg ccgctgtgac gccgcgcacc agagtcattc tgccggtgca ttacgccggc
    21061 gttccctgtg agatggatgt tattgcggat atcgcccgtc ggcatcagct gttcgtgatt
    21121 gaagacgcgg cgcacggcat cctgtgttcc tataaaggac ggccattggg aagcatcgga
    21181 catttcggcg cctgctcatt tcacgaaacg aaaagcatga tttcggggga aggcggcgca
    21241 ttgctgatca atgatgaaaa gtatgtcgaa cgggcggaaa tcatccggga aaagggcacc
    21301 aaccgcagtc agtttgttcg gaaaggctta agtcaataca cctgggttga tgtcgggtct
    21361 tcctatcttc ccagcgatat cattgccgca tttctttatg cccagatgga aaacgtcgat
    21421 caaatgatct cccgacgcaa tgcgatttat gatgtatatt ggaagcaatt ggagccgctg
    21481 gaggaaaaag ggctgatccg gcttccgact caacgcgacg attgtttccg caacggtcac
    21541 attttttatg tgatcaccgg atctttaaga gaaagaatcg acctggcctc ttttttgaaa
    21601 gaccggggga tctcggcctt ttttcattat gtgccgctgc acagttcccc ggccgggaaa
    21661 gtatacgcaa ggacgtgcgg cgccttggag gtaacgaccg cgatcagcaa ctgtcttttg
    21721 cgtttgcccc tttactacgc gatgacggaa gaagatgtcc aggctgttgt cagggccatc
    21781 atggattttt atcgggtccg ttgatgccgg atatgctgca gggtaaatga atttctattg
    21841 tgaaaaacac gagggggaaa atccattcta tggacgaaag ggaagaaaac ggaagcaacc
    21901 ccgttgctgc gggcaatgac gcccctcaac atcctttacg ggcgtcgtct ttttttgctt
    21961 tttctgaaaa actttcagca tcagtcaatc tgaggtggct gatgatctcc cttcttccgg
    22021 ttctgatttt actcaagtat ccggtggacc ggattgatta tgatgtgtgg tggcagatgg
    22081 cgcacgggca atattatctc acgcaccata cactgaaaat ggatctctca atgttttcct
    22141 ggacgccgac ggatccaacc tggatttata atacctgtct gggaagcatc gccgtttatc
    22201 tgttttacca ttgggcggag ggattcggct tgtggctttt tcaatggctt atattcagcg
    22261 gcgtttttct ttctttttac ctttttctgc gatcaatcca tcagcgtttg gatatcaaca
    22321 gcgcgacggt gatcgccgcc gttgccatgg cctgttccat ctcctgtcgt tattacaagc
    22381 ccgaattgtt gtccgcactc ttattctgtt ggacggcaca tatttttttt catatcaaga
    22441 tcaggggcaa ccggtatctt ttttatcttt tcccgccgat tttcgctttt tgggtcaatc
    22501 ttcacggagc ctttattgtc ggtctggcgt ttctcggcct ggcgtttgcc ggcgagtttc
    22561 tcaatagaat ttttttcccg aaaaaatcgt tgaagacgga agatctgatg catttcggcc
    22621 tggcccttat tctttccgga gcggccacgt tgctcaatcc ttatgggatt gactatttga
    22681 agagccttgt cccgacaatc atgaacgctg tcggcgtggg agattattcc ggcccgtatg
    22741 acaaatttgt tctggcctat gccagcctct ggccttattt aaggaaaatg gggcctgagt
    22801 tattcggtgt cggaatgacg gcctggatat tgacgctgat gatgatgagc attgcgtttc
    22861 ttgttgccta tgatctgatg aaacataaat cgtgtgactt tgctttatgc atagtcagtc
    22921 tggctttgta ttggaaaggc atggaaacaa gtcgggcaac ctattttttc ccgattgtct
    22981 ttttctttat cttttattat ctgctggttt cccgtttgca gatgaccaaa ttcggcagca
    23041 agacgacggt cttttcgatc atggttttca ttttcttttt cggcatggtt tcttttttta
    23101 acataaaata cggggcggac gataaatggt tcggaaggga tcttgacggt tacgtgccgg
    23161 ttgaggaagt tgaatttctg aaaaaatata aactgccggg accgatattc aacgattacg
    23221 ttatcggcgg atatttgttg tggaagcttt atcctgatta caaggtgttt attgatccgc
    23281 gcggcggcct ttacagaaat caggtatttc ccgattatat ggaattcacc atcagacatg
    23341 tcagccggga tgatatccgt cgattcagag agaaatatcc ttttaacatt gccatccttc
    23401 attacagaca gatggcgctt atttttgatt ttttaaaagc cggagacgac tggcgtttat
    23461 tatattttga aaaaaatgcg gccatcctga tacatcaatc gctgttgccc ctcattgagc
    23521 cgatagcggg caacatcagt ctcagcccga tccgtttcaa acatgtccgg aatccggaaa
    23581 ttctgacaaa tgtttttaac ttttatgtcc ggcttgatcc aagggcgggt agatacattt
    23641 attatgtctt caagagaaat gtaagtgacg gtttcttcat gaagaaggaa attttgaccg
    23701 ccatggattc caatatccgt ttaaaggaaa aggcgtttca ggaaaggccc gatttgtttt
    23761 tccatgaatg aaggaaaaat aatttgagca atgaaatatt tctagactta tgtcataaac
    23821 gggtcaatag tgataaagaa ccgattgagt catggaaagg atccctgcga ggtcatcatg
    23881 agttcggatg cattcaagat gtttatttcg gaaattgatc aggaacgttt tggtattaag
    23941 acagccagag ttgttgatat gacggcggat cgccttccct ctgtcctgga tttctgcgtc
    24001 agccatgcgg taaaattatt aattgcacgt tgcagtatat ctgatcttgg cgcggcgcaa
    24061 tccatggaaa agcaaggctt ccttctgatg gataccctcg tttattatac atttgatctt
    24121 ctgaggcggc cggtttcttc ttcagatgat gatgttcatt tcaggccgat ccggcgggga
    24181 gaagaaaacg ttgttgagcg ggtagcgata gagtcgtttc gcggctattt cggacattat
    24241 catgccgacc caagactgga ccgtgataaa tgcgatgacg tttatgtgga ctgggcaaga
    24301 aaagcctgcg ttgcaaaagg ttccgatgaa aattttatgg tagccgagat tcaaggcaga
    24361 atcgtagctt ttggcgtttt ccgcatgaat aatccggatg agggcgaatt gtttctggga
    24421 gggattcatc ccgatttccg ggggcagggc atctatcagt catttttgca cagagccaag
    24481 gcgtggtgct tgtcaaaaga cgccaggaga atggttattt ccacccagct caataatatt
    24541 gccgtgcaaa aaattctggt caaattcgga ttcgaaatca ccagagggtg ctatacattc
    24601 cataagtggt ttgattagcg ttttgcagtc tggtgttgaa agaacttata aagagaaaga
    24661 acaataattt tttctcatta cctggagtcg catgggcgaa aaggaaataa tttccgataa
    24721 cgatttattg aagactcaaa gtcggaaaag caaccgccga ccatcgataa cttcttgtct
    24781 ggttgccgtt tctgaaagac tggctgcttc ccccggtctg aaatggatga cagctatctt
    24841 agtgcccctg ctgatttttc tggcgtatcc gctgccgcgc ggcgattacg atttatggtg
    24901 gcagatggct ctgggaaaat attatttaac ccatcatacc ctggtgatcg atcattccat
    24961 tttttcctgg acgccggcgg attccggctg gatttataat acgtttcttg gaagcattat
    25021 tctttacctc atgtatcatt gcatgggcgg cttcggtcta tggttgtttc agtggctgat
    25081 ctttctcgga atttttcttt ctttttacct tttcctgcga tcgacaggcc aacgactgga
    25141 tgttacagcg ttaaccgtta ttgccgccat cggcatcacc tgttccatgg tttgtagttt
    25201 ttacaaaccg gagctgtttt cggccttatt gttttcctgg acggtcttta tctttttttc
    25261 cgtcaaagtg acccgaagaa aacatttatt ctattgttat ccagtgctct ttgcattttg
    25321 ggtcaacctg cacggtgctt ttattttagc ccttattttt cttgctctga tatttactgg
    25381 cgaaatttta aatagaataa tctttccgag agaatcattc accagcgatg aactgattca
    25441 ttttggcata gcatactttt tgtctctggc ggtgaccttg ttgaatcctt acggtattac
    25501 ttatcattgg agtatttacc atggattgac ctccgagatt tatgagctga acagcaaata
    25561 tatttcggcg tatgtctcct tatggcctta tttgaaaagc gtcagaagcc tttttgtcct
    25621 acagggtcag gtcgcctggc tgttcttttt gatgatgatg atcctcggat gtattttcct
    25681 ttttgatttt atcaagaaga aatcctgtga ttttgcgctg cttttcatct ttctggcaac
    25741 atcctgggga agcatgcggg gaatgagagg cggatacctt ctttctttta cttttttctt
    25801 catgtttgtg tattggattc accggttcca ttggaagacc ctgaccggaa aagcaaccct
    25861 catatcgttg atcatttttt tcttcctgtt tatcaatgtg gccaatcaga atatcagata
    25921 tggcgcctat aaccggtggt tcggagccgg gcttgacgag agtgtccctg ttgcagaagt
    25981 ggcgtttttg aagaagtata aactcgaagg gccgatattt aacgattacc tgctcggcgg
    26041 ttatctgatc tgggcgcttt atcccgatta taaggttttt attgacccgc gtcttgtgcc
    26101 ctatcataaa caggttgcac ctgattattg gcaactggaa cgcatgctgc caacccctga
    26161 ggacatgagg cgttttaatc aacgctatcc ttttaagaca gccatcattc attacaacag
    26221 acatttttta attgaagact ttttaaaggc gggatggaaa cttgtctatt tcgagaaaaa
    26281 tgccgctgtg ctggttcatg aatcaatgct ggccgccgtt ccgcccgaag tgcaatgggt
    26341 tgacctgggg ccctcgcgtt tcaggaatgt gaaggatccg gtggttttgc tgaatgtctt
    26401 caatatttac gtgaacgtat atcccgaggc aagccgggtt atttatgata tttacagaag
    26461 caatgtcagc acatggtacg aaccgagaga gcagcatctc cgcgcgatgg aagaggatat
    26521 gcggaaaatg caatcactcc agcgtaaagc caaatcgtaa gcactccttt gacccgacca
    26581 accggagtcc gattccaatc ttattcgctg tcttcaataa aataatactt gtttttataa
    26641 cgcatattat tcatgaaatt agcggatgaa tccaccggtt ctttaagatc tgcaaccttg
    26701 ctgcccttgg atgaaccgaa tttaaaaggc agtaaaccaa aaatcatatc ggtcacgata
    26761 tgaaggggca ttgtggcttt caacgaggcc aggtaaacag agacttcctg ttcataactc
    26821 aacccccggt aagatgtatt cagaccgtct cccagcgtat aggagttcat gtttttactt
    26881 tgcccgggtc tgattttaaa tgatttactg atcatataac ccagcgcgag gtcgatatcg
    26941 atatctttcc attgaattcc cataccggcg ccgaaatagc gcatggaggg aagggcgtac
    27001 ataagatcgt aatattcatc aatagttgaa ctcgtacggt tttcgtaacc ggcccgcaat
    27061 tggagccaat ccagcaactg gtattcaaca cccacgcccc agttcagcgt atccttgaat
    27121 tctctggcca ctttcatatt gtatgatcct ccgctgtaac ccatgaattt ggccatctga
    27181 agaaattgaa ccttttgatc aaatttgaga tcgttttcct tgaccgcgga ccactccgcc
    27241 cagtgcactt cgcctaaaag agacagtcgc tttgtcggtt tgagtttgac gccgacattg
    27301 actatttgag gaaattccag agtcgtcgag acggttccct tttgttcatc tgtggccgtg
    27361 taaggcaggt caaaaatgat cgacgaaatc tgcatgacag cggtactgcc cgaccaggcc
    27421 accattcttt gccagtcttc actgtatttc agactgaatt tgccggatac atttgattta
    27481 acggcactct gataacaaag tccaagcgct acccagtcaa agggttccca caatactccc
    27541 agattataag agggagaaaa atcatctctt aagctcaatg ataaatcgcc tatattatca
    27601 taaaccccga cgccgccggc gaaaagcggc aggggaaccg tgacatcgaa aagcggattg
    27661 gccatgccct gtgttgcgtc ccccagaatt ttcgttatgt tgacgatttc attgggacct
    27721 ctcattttca gttccagtcc catggctgtc tgcccgatgc cgaacgaggc gcccacggcc
    27781 agattgttgt tgatccggta gctgatggcc ggagcggcat aaatcagatg ctgcagatac
    27841 acggattttg ctccgtacat cgccggatca tcgtcgccat attcccatcc accgccgtac
    27901 tgggcatagg tcgtgtatcc aaacgtccat ttggagccgg gctttctgta ggataagccg
    27961 aatatcggac ccataacggc cggcgccgtg tcgttcagaa tgggaatgaa cattctgccc
    28021 gtgctggtgc tgctggatct tccggcaagc ggatcaacaa tgacatttcc ctgataatca
    28081 tgaaaggcct gatggttggg gtcggattca aatgtccctg acattttgag aaccgcagga
    28141 atgaggcccg cactgatgta actgccgtcc cccataaaag agagtccggc agggttataa
    28201 tgaatagatg cggtccccgg gggatcagcc gtcacgttgt tggccagtga aatggctttg
    28261 gtgtcaatgg ccatggattc attgaaagcg ccttctgcct ggtgcaggcc gaaaaataaa
    28321 accgccgcca atgtaatgaa caaagaatag atcggcacat acaaaagcaa tttattcgaa
    28381 tttaaaattt tccttcggaa aaaagataat accggtaaag ataatgataa ataggtaatc
    28441 aaccttttga tctttgaaag tattgtcatc atccctcccc cattactatg ttgtaagata
    28501 aaacgtaaaa aaacattttt ttctgatcgt taaaaagttc ttatagagat tctcctaaaa
    28561 ttcatacggt aaccgcaaag ataaagacac gtcgggatcg ttattggtca gccccatgct
    28621 gacagttgca tatattgatc ttgaaggagt tattctccaa ccggttccca cgttaaatga
    28681 tgaagagagg gaactgcctg tttcagtggt gccggtgttg gatttgtatt tgctgccgaa
    28741 ggaataactg aattgcgcgc tcatattcaa agaagcctga taggacaggg cataaccgaa
    28801 tcccatggac actccgatgc tgcttcccgg ctcgactttt tccagatata ttttcgatgc
    28861 cgggtcagaa ggatcctcat acagaatttg agacaggccg ctttcgggta tgccgtaggt
    28921 gtagtttaaa tttccgaagg cgaccagcgg gtccaacacc ttgctgaacg aaacccccgc
    28981 gccaattccg taatagccgg aaccggttgc cagttcgtct tcataattaa tttcgaaggg
    29041 gctgcttcct gtggggaggg acaaactcaa tgataatatg gttgtgggga tgcgacctcc
    29101 ggctttaaac ggctgccacg agaatccgat agagatatct cccagatccg ttacgtcaag
    29161 agattcatcg gtgcccactt tattgtattt gtaaacgaaa ggaaaattag aactgacggt
    29221 gagattatta aaaaaagcgt attcgagagc aagggtattg gtcagattat gattgactct
    29281 gcgcaaaacc gttgttgatt catcaatggc atcgccggag taatacgcat agttgaacgt
    29341 ataggacagc ccgacagtgc ctttcttcaa aagagaatac tgccggcctg cggcagaaag
    29401 tatgtcgtca atggtctggc ttttttcttc ttcggggatt tccagctttt tgcgcgcttc
    29461 gttttctccc cgtaattttt gaatttcctc tttaaggtct tttaactcct gctcgatgct
    29521 ctttgccgat ccctcctgag tcgtgactgg cgtttgcggt tcggcaacgg gaggagcttc
    29581 gaccgctttt gtttcttgaa cgtccgttgt ttctttcgga gctttgactg cctcctgatc
    29641 agcggccgca tttgcatcgt ccgcgctgac accgcttaac gcaagctgaa tcccctcaag
    29701 aggttcattg gccataaccg gcagggccgc aagaggggta aaggttaaaa aaaataataa
    29761 tgacaaaaca atgacaaatt ttcttttcat caatatcctt tgggttaagt agtttggtta
    29821 cgtgataata tacttttaaa ataaaatatg ccgagaaacg ccatcactac ttttcttcat
    29881 gtcatttatc gaatgcataa aaatatttac ctttttctta aaaaacatat tgaaaagcga
    29941 aaaaagctcg tcaaaatttt ttaacaactc ctccccgttt cttaaatcac acagttacct
    30001 ttttgttaaa agccggtgtt taaagaaaaa aagggctttt gattgaagca ataaacttat
    30061 ttttacaaag tggcgaaata atatacaagc atgtctcggt tgtcaaggag aaacagcatt
    30121 ttatgaatga aaatataaac gtttttttaa gcacttaagc cctatagaac aattttttta
    30181 cagggatgcc cgtactttga aatcgctaat gaaaacaaca gcgctttgga gcgcttcgtt
    30241 tctttaaata ttggatatta aagagcatta tttttttatt acgtcaataa ttgtgatttc
    30301 cggaggtgca aggaaacgaa cgggcggccc ccatgtgccc gctcccttgc ttatataaac
    30361 ggatttgtta tcggataatt gatgatatcc gctgtttttt gggaaaaaaa tcctcgtcag
    30421 gaacataaat gggaaaattt gtcccccatg ggtatggccg gaaagctgca agtcgaaatt
    30481 tttatcttct ctgatggtcg gctggtgttt taacaataaa ataaactctt tacttttttt
    30541 aaccgggaac agattggata agctggggtt tttttctgaa acaccgtaac cgcgtccggt
    30601 cggatcatcc agcccgatca tatgtattcc ggctattttt ttgctctcat ctcttagtat
    30661 ttcaaaaccg gcactttttg taaatgccag agatttttca attcccgcgt aatattcatg
    30721 attgcccatg atggcgtatt ttccaaactt tggttttatt tccatcattc tttttgcttc
    30781 agacgcgata ttgtccagtt ctccgtccag caaatctccc gtggaaacca gaatgtccgg
    30841 ttttgcctcc cgtacctttt caaggatgtt ttgcaggcgt ttacccctga caatcagccc
    30901 gatgtgaacg tcggatatct gcacaatacg taatgtttca tgcccgggcg gtaatttttc
    30961 agtatggatt tccagctttt tgacttgtat gtttttcgcg tcgaagaacc cgtacaccac
    31021 aaaagacaaa gaaaggaaaa gagccgtgaa aaaaagaatg ttttttaaag agtcgcctcc
    31081 cggggtctga aatattattt ttaataacgg ccggatcaga tcaaaggaaa catgaataac
    31141 gaaaaataaa aaaacaaaag ccatccacag gtacccgcaa taagctattg atcgggccag
    31201 cgtttcccaa tgaatttttt ccgccagttt tatgataatc ggagccagaa taagaaaaat
    31261 cattgaaata attatgatga tttcaatttt gccggaaaag tggaagagag tttttgcctt
    31321 cagaaaagcg taaaaattaa tgccgccgta aagacttaaa aaaatcagta ggaaaagaat
    31381 catggtctcg accttgcttg caaaaagatg taaaaacttg acaatcaata acccttaaga
    31441 taagagtttg acctctttat aagaggctga ttcattccgg atgccgatgt agctcagccg
    31501 gtagagcaac tgattcgtaa tcagtaggtc accagttcga ttctggtcat cggctccaga
    31561 gaatcaatga gccccgtcat cgggaatcat cttttttaac tacacagaat tttaaaatcc
    31621 gtcaacataa caaaagactt gatcctctgt gcatggactc ataaaccgga gggagataaa
    31681 ccgctgtaaa gggttaactg gtatgacgtt gataccgata attttcatgc gattaagata
    31741 gagaaaaaat catctgatga ttttaaaaat tctgtatgcg atatatttca atttatccag
    31801 agatttggtg gcccgaagaa cattccgaat gactttcggc cgcaaataaa aattaataaa
    31861 tgccagcgcg aataaccacg taaattgctg gcgggaaatg ccttccggaa cgtaaggttt
    31921 catatcctgt cccatgtttt gccagttcat tggtccaagt tttttttctg ctatcagcct
    31981 ttcgtaaatt tcagtgcccg gcgtgggatg aaacgggaag aagttggcgc catatagagg
    32041 aagctttccg gcgaatttta tggttttgcg gatgtcgtct tcattctcag caggataacc
    32101 gatgataaaa ttgccgatca cgttgatacc gtttttagtt ttaatcagtt ttaatttttc
    32161 ttcaatcaga ttaagcgtca gctttttttt catatgatcg agaatccgct gggatccgga
    32221 ttcgataccc acggttaata aatagcagcc ggatttttcc atcaggtcca gcacctgttc
    32281 gtcaaccagg tccagacgaa tgccgaaaca agaccaccgg atgtccaggt tttcagcaag
    32341 gatcttttca cacaactcca tgaccagatc tttccttgcc agaaaattat cgtcaatgaa
    32401 acatacctct ctgatgccga agcgcgattg cagtagcttg atttgatgga tcatgtagga
    32461 aacgctgtgg gccctgatct tatggccggt gattgcgcgg caggaacaga aggtgcagtg
    32521 gtaagggcat ccgcgggtca taataagcgg cgcgacgatt tttgaccggg caaagtaaga
    32581 tgtctggaaa ggataatggc agggattgat taaatcccaa tccggcgaac cgagggcgtc
    32641 aagattttcg atgaacaagg gaggattgca cctgatggct ccgctttctc tccagatcaa
    32701 tccgggaatc tgatcttttg caatttgatc cggctgagac aggtgcgtga ttaaatcggt
    32761 cagtcctttt tccgcttcac ctctgatggc aaaatcggct tcttcgaaat attgcagcgt
    32821 ctgttcggga aacgt
//
LOCUS       DAONQZ010000021        32805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1484_length_32805_cov_17.321832, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000021 DAONQZ010000000
VERSION     DAONQZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32805
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_32805_cov_17.321832"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..570)
                     /locus_tag="PLR13_05395"
     CDS             complement(28..570)
                     /locus_tag="PLR13_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18803.1"
                     /translation="MPANEPQKLPKTWTTEMLNAFHKLINSPLPVSFTVSEKNKLTTF
                     FGKQNPSSHARVACHVSYKGNISVPEFENVGVVFNPAKNLTDEYKNFIGYIEIVNGLK
                     EDTIPVPEIRVFIQEDSGVCDVFSEMLIESKTLHAKLSPIYIYLEQSYCLKDKPPEEL
                     LKKRIPIKEISTFQKISCVE"
     gene            complement(906..1739)
                     /locus_tag="PLR13_05400"
     CDS             complement(906..1739)
                     /locus_tag="PLR13_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18804.1"
                     /translation="MRLERETKEFFEKYRQRYLDNRQRFSDGLNKTLAEKKDWIAHIS
                     YQPYKWLFVLPLLIATTLICGVLAIVTALLFSPRTGGIFGMIWARINSFCTPMTVHVV
                     GADNIEPGTSYVITSNHQSMYDIYVLYGWLGIDFKWVMKKELKKVPVLGLACKIIGHI
                     YIDRSDSKSAVDTIKAARAKIVGGTSVLFFPEGTRSVDGKMKQFKKGAFKMAIDLGIP
                     ILPVTINGTRNILPKGSLDLTPGGATMIIHKPIPVTDYHDGNLPELMEHTRRRMNAFL
                     D"
     gene            complement(1777..2265)
                     /locus_tag="PLR13_05405"
     CDS             complement(1777..2265)
                     /locus_tag="PLR13_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18805.1"
                     /translation="MAQKITISVPDELYEKMQAWKSSLNFSRVFQNAVSKIIQKKENL
                     TSKIRNEIEFSSIVDRLKKEKVDYELNIAEWGKKDGLEWCKTAHYREIQYALAWTACE
                     NPNQDEELGDYFSDIFKKYKAQVAATGKKAQEAWSDFSEKYLKGWREGVELFWHEVKD
                     KL"
     gene            complement(2625..4526)
                     /locus_tag="PLR13_05410"
     CDS             complement(2625..4526)
                     /locus_tag="PLR13_05410"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HQC18806.1"
                     /translation="MADKSKYAGVYSESINQPDIFWGKAAEDVVWNKKWDKVLDDSKK
                     PFYRWFSGGELNTCYNALDYQVESGRGEQNAIIYDSPVTNTVQKITYKELLDKVSKFA
                     GAISALGVKKGDTVIIYMPMIPEALVAVLSCARIGAVHSVVFGGFAPNELAIRIDDAK
                     PKIIISASCGIEGKKTIPYKPLLDEAIKIAAHKPQKCVIYQRPQVKADMDSARDLDFN
                     DVMKNAKAAGCVSVAATDPLYILYTSGTTGKPKGVMRDNGGHAVALKWSMKNIYDVKP
                     GDVFWAASDVGWVVGHSYIIYAPLLYGCTTIVYEGKPVGTPDPGAFWRVISQHGVSVL
                     FTAPTAFRAIKKEDPNGDYLKKYDIKCLRYLFLAGERLDPDTYHWASDMLKIPVVDHW
                     WQTETGWPIAANCMGIERFPIKAGSPTKPVPGYNVQILDGDGNQLPNGEEGSVVIKLP
                     LPPGCLPTLWNDDKRYLDYVMERPGYYVTGDGGRFDQDGYIFIMGRIDDVINVAGHRL
                     STGAMEEIISKHKDVAECAVFGAEDQLKGQVPVGFVVLKAGVDRDPDDIVRELVQMVR
                     SELGALACFKEAAIVKALPKTRSGKTLRSTMRKIVDGKEYVIPSTIDDPSVLGSIAEA
                     AKTIGYGKK"
     gene            complement(4808..5971)
                     /locus_tag="PLR13_05415"
     CDS             complement(4808..5971)
                     /locus_tag="PLR13_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQC18807.1"
                     /translation="MKLDDIKKIAVIGSGAMGNGIAQVCAQAGIKAVMVDIKQEFLDK
                     GMATVNKSLDTLVGKGKMTAEKKAEVMGNLSTSLSNTEAVKDVQVIIEAVPEIMSLKK
                     TVCAEISAAAPADALIASNTSTMSISEIATAGKNPERILGMHFFNPAPVMKGVEVIYA
                     KQTSDANVDLLCELTKKIGKVPIKVLKDSPGFIVNRIGAPNQALISAILDEGKIKVDT
                     IDAVMKAKAGMPMGPFELADYVGIDVFYHTLKYYSETLSPEFKPGKTLQTLLDSGKLG
                     MKSGQGIYTWEGGKAKIDMSVESQDFGPLDFLAIQINEAVRVYKEKIAASAADIDLGM
                     VHCMRAFAGPFAFGAGMEPAQLADTLNKLHTRFGLNIFKPEPEIVDGSFKNMK"
     gene            6359..6655
                     /locus_tag="PLR13_05420"
     CDS             6359..6655
                     /locus_tag="PLR13_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18808.1"
                     /translation="MNSLKKAIFIVCFFNLLGLWSLSLSGVAIANQNEQEASLEKCIK
                     SCEDSKQVCWNMDPDRRLCEAQFQLCMENCNKKYNAPVVEDVKPPEPKKTRTLH"
     gene            6944..7897
                     /locus_tag="PLR13_05425"
     CDS             6944..7897
                     /locus_tag="PLR13_05425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQC18809.1"
                     /translation="MKDDKNIAAVFPGQGSQRPGMGKDFYEQIPVCRQTFEEAADALG
                     WNIMDMCFGEDERLNLTEYTQPCIVTTEICMLRGLNERYGFAPDYFGGHSLGEFTALV
                     AAGVLPLFDTLKIVQIRGRLMQDAVPVGVGGMAAVISENIDMNMLTKTMEGLKAGIAN
                     INSANQVVISGELPAIDEAEKRFTENFPEGKSFRFIRLNVSAPFHSTLMKTIEGRFAE
                     TLEKFGSHLNPVNAPRVTSNFTGGFHAGDKADVKKNLVHQISHTVNWRKNMQALAENT
                     QAIYEIGPGRPLRDFFKTIGVTCQSITGLNAAEKVFKATCQ"
     gene            7940..9385
                     /locus_tag="PLR13_05430"
     CDS             7940..9385
                     /locus_tag="PLR13_05430"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HQC18810.1"
                     /translation="MIQEKDIKNIPLKIQDNTFRDGHQSIYATRMRTEDMIPIAEEMD
                     QCGFHAMEVWGGATFDTMHRFLGEDPWARPRILKQYIKKTPFAMLIRGQNLVGYRHYA
                     DDVVRAFVDKACEIGIDVFRCFDALNDFRNMESCAERIKANGKIFQGAVCYSLTDVRL
                     GGDVYNLDYYVRKAKALEQMGANAICIKDMGGIMSPFDAYDLVTAIKKEINLPLHLHS
                     HYTSGMASMTYLKAIEAGVDVVDTCLAPFALRTSMPAIEPIVAALRGTSRDTGIDLYK
                     LLALGEHLENVAPKYNSHLATHKLSIIDNGVLAHQIPGGMISNLTGQLKEMNALDRLP
                     EIYKEVVQTRKDMGSPPLVTPVSQIIGAQAVLNVLFGRYVRVSNQLKDLALGLYGKTP
                     MPIDAALTKKILKNYKRGQEPVSGRPADYLEPEIEAAKKAMGDLARSDEDVLAYILYP
                     ATMTKFLQSKYGIEPPRATGDDNAQKKGAAA"
     gene            9491..10690
                     /locus_tag="PLR13_05435"
     CDS             9491..10690
                     /locus_tag="PLR13_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC18811.1"
                     /translation="MDFSLNESQLSYLDTVRKFVKNEITPRILDMEKEHVFPWEIINK
                     AWKTGLINLSIPESVRGYEMDLFTSALVVEGLSYGDTGISTSAMCNDLANVVIGLHGT
                     DEQKNLFLKPFVEKPILASFCLTEPNAGSDNSMMAAFISKREDGKYILNGAKCFITNA
                     SYASQFTVLCKTGKPTGNFMACVIVPVEHVTSYDIPDIGNATREISVPAGGKIIIGKP
                     EDKLGQRLSNTASVTFEDVVVFPNHIIGDRRLGFKYIIDVLDYARPMVAAIGLGLAKR
                     AFDLTVEYTRERKQFGHRICDLPVAREMLTTMWKKVELAEMALFKAIFKIQEKAQDAG
                     IYASLAKNTAAEAAVFCATEGLHLHGGYGFTTEYEISKLARDAHIIDIYEGVREVQNM
                     IIGREII"
     gene            10690..11682
                     /locus_tag="PLR13_05440"
     CDS             10690..11682
                     /locus_tag="PLR13_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQC18812.1"
                     /translation="MLYLHAMGHFHPENVISNQFLEELDMGTSNDWIMERVGIQNRRT
                     VLPLDYIRKTKNVHPLQSFSIRLYTNAQMSAMAARMALQRAGLKAKDIGLIISGSSSP
                     DNITPAEAGAVAAELGIEVPCFDLNSACSTFGMQVNFLLRMKPEEMPPYVLLVGAEST
                     TKVINYTDRNSSVLFGDGAHAAVVSSTIPARAAFISGGYNARPSGWTKVGVTGDWVFS
                     QDGNAVQGFAIRTTTECLKKLQDQYEAQAKRFIFIAHQANYMMLKTVCERRHIKPEHH
                     WFNVDQFGNVGCCGAPAVLSMNWDNIRPGDNIALVIVGAGLAWAYAMLKVEDKQ"
     gene            11679..12407
                     /locus_tag="PLR13_05445"
     CDS             11679..12407
                     /locus_tag="PLR13_05445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HQC18813.1"
                     /translation="MIERPVALVTGGAKGIGAACCRALAAEGFHVGIHYNQSTASAQT
                     LRDQIREGFLIQADLSDIGQVDAMIGKLKDTTGRVDVLVNNAGRSVNADIISMKVEQF
                     DEQRTLTRGVWYLTKRILRQFMFRNSAGRIINISSVVGHTGNAGQIPYTMEKAALDAF
                     TRSLARELKGRNILVNSVAPGFIDTEMTKDLPEEIKNKAMEDILLGRVGRPEEIAEVV
                     AFLAKKGSYINGSVIHVNGGLYGG"
     gene            12397..12867
                     /locus_tag="PLR13_05450"
     CDS             12397..12867
                     /locus_tag="PLR13_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HQC18814.1"
                     /translation="MEADAALKQEVLSLVPQQPPFRFIDEIISLDDEQIIGAYRFRED
                     EYFYRGHFPGNPITPGVILIETMAQVGVVAFGIYLLSRQKNMRLHDMKLPISLFSLAD
                     GVEFKQIVKPGERVIVRAKKIYFRKMTIKAAVSMEKENGEMICSGELSGVGVEQ"
     gene            12864..14144
                     /locus_tag="PLR13_05455"
     CDS             12864..14144
                     /locus_tag="PLR13_05455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQC18815.1"
                     /translation="MKKRIVITGMGVAAPNAHGLDNFEKALRNGVSGIRFIPRLQELN
                     FGCQIAGVPEYFDEIRSSYFDREKLLSINDNIGYASVSAVDAWTDAGFALKDDDDDTV
                     DWDTGVIAGSGIGGMDTIANTIVPLINEGKVRRLGSRVVEQVMGSGTSARISGLLGVG
                     NQVTSNSSACSTGNEAIIDAMGRIRTGLAKRMIAGGSEGATPYIWGGFDAMRVLCRKF
                     NDNPEAGSRPLSASAAGFVPGAGGAMLVLEELETALGRGARIYAEILGGYVNCGGQRM
                     GGSMTAPNPEGVKRCIRGAVGDAGIDPAQVDAINGHLTATYADPYEVKNWAEALERTP
                     ENFPYIQSTKSLIGHCLGAAGAIECVAVVLELFKEFLHPSVNSGDVHPEIEKFAPKIP
                     QKCLDVPEIKIIAKAGFGFGDVNSCLIFKKWEGK"
     gene            14171..14419
                     /locus_tag="PLR13_05460"
     CDS             14171..14419
                     /locus_tag="PLR13_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQC18816.1"
                     /translation="MDDKKIFEEMINILRPYAKNQDRLATATLETHILNDLKVNSARL
                     VDVIIKCEDVYGISVDDDEADKIRTIGDAVRIIKQKLV"
     gene            14423..15247
                     /locus_tag="PLR13_05465"
     CDS             14423..15247
                     /locus_tag="PLR13_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18817.1"
                     /translation="MTRKSLILLNMQKFIGRMAIFILAPLYFFAAKMLFYRVRDLKKT
                     RSLCAAEFAKHKGGWIICSNHLTMIDSFILSYAMFSLPQHIIHYKKMPWNLPEWTNYK
                     DNILLTILCYLSKCIPINRGGAREKMKESLEKCMYLLKSGETIMIFPEGKRSRTGRVD
                     QEGFSYGVGRFIKHLEDCNVMCIYLRGDQQDHYGVIPAWGEKFSVQMEVFKPHPVEGS
                     DLRVQREYARQIVEKLAAMEEGYFALCRKRCGGFEGCGELGKKQGFALSKKNPHRC"
     gene            15171..15776
                     /locus_tag="PLR13_05470"
     CDS             15171..15776
                     /locus_tag="PLR13_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQC18818.1"
                     /translation="MDLKDAANSGKSRDSRFLKKILTDAEIEYVTHADHPDSALWLLW
                     ACKETAYKVIKKILPDIVFAPRQWQVNVHNIKSEYAEGEVGIPGKDGVFIRLFAGADY
                     IHCAGSDDKATLDNLIWSVEPLPDEETCPSLFSRRCLAQSLAGRFSLDFHQIKIQRKR
                     RNGELQPPYVYVDGRQTDTEISLSHDGRFVAYAFGSTAQRK"
     assembly_gap    16036..16135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16295..16666
                     /locus_tag="PLR13_05475"
     CDS             16295..16666
                     /locus_tag="PLR13_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC18819.1"
                     /translation="MNEKRKASRLMEESEVTITLIAGGKKFFREKVIYNSSKDISESG
                     ARVHSSIFLPIDTLLKIDFKLKNLQHMITAMGKVKWIKIICEGEAYDAGVEFVNTPTE
                     AIRKIADYIAWRQKQKTTKRH"
     gene            16819..17013
                     /locus_tag="PLR13_05480"
     CDS             16819..17013
                     /locus_tag="PLR13_05480"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQC18820.1"
                     /translation="MEQNMGTVDKVIRTIVAILIIVAYLMGMLPGIAGLLLIISGSLL
                     SSVVSGYCPLYTKIGVNTNR"
     gene            17127..18506
                     /locus_tag="PLR13_05485"
     CDS             17127..18506
                     /locus_tag="PLR13_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQC18821.1"
                     /translation="MNIAVLNGSPKGDVSVTMQYVHFLQQRFPETEFRIFDVALKIKK
                     LENDDRALQEVIANVADADAVLWAFPLYFLLVCSQYKRFIELIFEKNAVSAFHGKPAA
                     CLTTSINFFDYTAHNYIHAVSDDLGMNYFGSYSAEMNDLLKTKERRRLKTFGELFLKA
                     IREKTPAIRSYKPLVAPEIVFDTEAIRPVDAAGKKVLILADDLAANSNLRKMVERLQK
                     TFSPAAELVQLKDVDIKGGCLGCIKCGFNNVCVYEGKDGYIDFFNHKIKPADIVFLAG
                     AIHDRYLSSLWKCFFDRSFFNGHTPFLKDKQIGFVISGPFGQIHNLREIFDATTKFQL
                     AGLAGFVSDDTGGNPEINAALQTLAVQAAEQSRLGYIQPPTFYQVAGAKLFRDVIYGR
                     LRPVFQADHRYYKEHGMYNFPQKYYKWRMINSTLVFLTKIPAFRREFFKRIKKEMIKP
                     AAGALKKTE"
     gene            18557..19324
                     /locus_tag="PLR13_05490"
     CDS             18557..19324
                     /locus_tag="PLR13_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HQC18822.1"
                     /translation="MNHQDVVAFVTGGASGLGEASVLELIKKGAAVVIADVDTEKGES
                     LAAGIGSKAFFVKADVTSETEIRQAIEKAVEIFGKINVVVNCAGIVSPGKLVGRKGPL
                     PLEAFNKVLQINLVGTLNVIRLAVEQMMKNTPGEEGEKGVIINSASIAAFDGQVGQVS
                     YSASKAGIVGMTLPIAREISEYGLRIVTVAPGIFDTPMMAGLPTTVKDDMAQMVPFPK
                     RLGKPVEFARMVLHIIENTMLNGCCIRLDGALRMPPK"
     gene            19945..>20345
                     /locus_tag="PLR13_05495"
     CDS             19945..>20345
                     /locus_tag="PLR13_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQC18823.1"
                     /translation="MAEANEQDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESITDPN
                     AFWAKMANERLTWFKPFDKKKVSDWSFDAKDVHSKWFEGGKINVSYNCLDRHLATKKN
                     KAAIIFEGNDPNDWKVYTYYDMYREVNKFANV"
     assembly_gap    20346..20445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20446..21699
                     /locus_tag="PLR13_05500"
     CDS             <20446..21699
                     /locus_tag="PLR13_05500"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQC18824.1"
                     /translation="DIWYEKLMADAKPYCKPEEMDAEDPLFILYTSGSTGQPKGVVHT
                     QAGYLLYASMTQQLAFDVREDDTYWCTADIGWVTGHSYIVYGPLCNGHSSILFEGVPN
                     YPDFGRFWAVVEKYNVNNFYTAPTAIRSIAKEGDKWVDMHDISSLRVLGSVGEPLNPE
                     AWWWYYNKIGGGRCTISDTWWQTETGGHMILPLPGAIDIKPAKAMVPFFGVVPVLMDE
                     EGKEIKGVGKGALCIKNSWPGIARGLWDDKEGRFVENYFSQFPGHYFSGDGAERDADG
                     DYKITGRVDDVINVSGHRLGTAEVEAALTSHPDVAEAAVVGFPHDIKGQGIFAYVTLN
                     NNVAKTDDLKKALVAHVRKVVGPIASPDQIQWADGLPKTRSGKIMRRVLRNVAAKTFG
                     DFGDTSTLADPAVVDDLVNNRKKLG"
     gene            complement(21825..22931)
                     /locus_tag="PLR13_05505"
     CDS             complement(21825..22931)
                     /locus_tag="PLR13_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC18825.1"
                     /translation="MEERRKSLRIKEENEVTITVFSEENNIPKEKIIENFTKDISAGG
                     AKLQTNILMPVDTLIELEFTPKGLRQQINAIGKVKWVKVLIDDESYQAGVEFSGPPNE
                     AIGKLQDYISWKLKSHKVDYIKEKLSPVDSGNVNIEKTPEIAPIDPAAIEITKDDTIP
                     RDQSGQKDTEKIKREPRARNKKWIALPIFVFFVLVLAAVLLQAFGVIPSLNNLLYPET
                     KLSKTAIKNNEILQKPAVESGVPAENLPAEAPAATEPVSEETVIKNMLDKWINSWKSG
                     DMEAYRSCYAPDFQSKEKDLNAWITHKANIFKNSKDIAISINDVRIHLNGDSAKVLFI
                     QQYSSSLIKSSGKKILHLKKIDNGWRIHKELMRP"
     gene            complement(23151..25832)
                     /locus_tag="PLR13_05510"
     CDS             complement(23151..25832)
                     /locus_tag="PLR13_05510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC18826.1"
                     /translation="MNQEFSTSEKAQSTKKNVQSDSSFARALLKDELAETIHQAVRDN
                     RSYLMEHEAKEILEGIGIRTTGFLVARSEDEVLAMSEKIGYPVVLKIVSPDVVHKTDA
                     GGVKLNLHSAGDVRTAYQDILEKFKYQHIEGVTVQKMAKPGIEAIIGVTRDPSFGPLI
                     MFGLGGVFVEVLRDVSFRILPITEKDASEMIAEIRGAAILEGYRGQAVDLDSLSNLLM
                     KISQLVIENPEISELDLNPLFLYPDGYITVDARMFVKETSPGENVTATGRESLRDFFY
                     PKSIAVIGASDVKGKLGYNVFWNLINHDFPGMLYPINPKKDSIKGVKCYKSILDVPDP
                     VDMAIVIVPAKAVEAAISDCCSKGVKYVVVEAAGFAETGEEGRQAQERIEKIIRAHGC
                     RVLGPNCSGVINTHHNMVQSIGLLSDLHKGNVGMVAQAGVYAAGILTGLSNVLDFGIV
                     ATIGNKMDINETDILEYLSDDDNISVIVMYMEDIRSGKRFVDVASRAAVRKPVIILKS
                     GRTEAGKKAVSSHTASLAGNDEINSAAFKQSGIIRARDNEHLFALTRAFSKQPVPKGN
                     GVLVITYTGSMGVAATDMLYLSNMRLAALEPYFQKRLKQVMPDYASIGNPIDCSFSMT
                     PRQVKDLIDIGVESGDVHGFIVIIQGEILGSFVDVMKNIDYKEKPVLCCVACKEFMID
                     DVVKMEQAGIPVYSTAEMAAEVLSQMYHYGVRRQSAIADSIARHITRNSLSVDNRAVR
                     LRLINPKDIGLWTDFVNNCSEKSLWLRFLSPFSATPERAQRFCNVNPEEEIAVVAEMK
                     DGDKYKFLGIARLIKNKRCEGEAEYAIIVSDNWQNKSLGHTLSEQCIELARKEGYKTI
                     RAETLQENYAMLRIFRHCDFVLDSKDENMVSMTLNLV"
     gene            complement(26041..26673)
                     /locus_tag="PLR13_05515"
     CDS             complement(26041..26673)
                     /locus_tag="PLR13_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQC18827.1"
                     /translation="MIRILVADDHAVVRQGVKQILADVTDMTVQDEAQNGPETLKKVT
                     DNNYDVVLLDISMPGRSGLEVLEDIKAQHPKLAVLILSMHPEEQYAVRALRAGASGYL
                     TKASAPQELIGAIRKVAGGGKYVTSSLAEKLADELEIDTEKLPHERLSNREHQVMLML
                     AEGKSVSDIADELCLSVKTISTYRTRVMSKMGMKKNAELTLYAVHNKLIG"
     gene            complement(26670..28172)
                     /locus_tag="PLR13_05520"
     CDS             complement(26670..28172)
                     /locus_tag="PLR13_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18828.1"
                     /translation="MSKKQLIELVSSLQAQLVSADASSLSGTSGEDLYRFLAQSSQVG
                     FYIMQDGRFRFINSHFRDYAGYTEEEMLKMDPISLILPEDRRKTAKSAVMMLKEQRFS
                     PYEFRIVTRDGRIKWIMETVMSIKFKGGRAILGNSMDVTERKEAGRRLEELEALESSI
                     LDAIPQAVVGLHERRINFANHAVKIIFGWHREELIGKSIRMIYRNEEEADTIGDLFYS
                     TLEKQRTYETEFPCRCKDGREIICRMKASRIGEKLREKRIVVTFEDITAQKKAQEDLE
                     RSRQDLRNLSAHLQSVREEESTRMARKIHDELGQSLTALQMDLSWLKTRLPTSSNSIR
                     EKTKSMSELVDATIESVHTITTELRPSLLDDLGLPAAIEWQAGDFQKRSGIRCQAYIT
                     GNMETISKDLATSIFRIFQETLTNIARHSKATQCKVALSERGKELCLEVTDNGIGITQ
                     WQIDDSRSFGIIGMRERAHLWDGTVHVRNAKPSGTTVRVLIPLEKGEKLS"
     gene            complement(28406..29686)
                     /locus_tag="PLR13_05525"
     CDS             complement(28406..29686)
                     /locus_tag="PLR13_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC18829.1"
                     /translation="MIEFIGYFCASLAIASSLIGLLSVFIFRSHIKHWTRKTPATYPA
                     LTVISPQRGKIDPENVEAILGQNYPGVWEVIFVTTKDDASLPQLQHYLQKYKNVKIAI
                     AEDVVELATTKGIHRCQKNNNLLTAIKAASPQTEVYAIIDADARPFSDWLSCLVAPLT
                     DANSRLGVVTSARIYLPGKGLASLVQVLWILISAMFLVGKCRYIWGGGLAIPKKVFDN
                     ANLARQINGQGGSSITTDDMNIYNALRKQGYESLFVPDCIVLRHPPEKKENLANVITF
                     TNRQVLQTWWTTRGIWVLLCNIGIRLPMLLCALVIAWWYPWCLLALLSIVIDVFMGIS
                     ALKTVIDAEPRLSAKLIFWIMKSKGAIAGRLLQPEIRIQVNFWIVLLPIVTPFVVTLN
                     TFTVPFFRKMYWSGIAYTRRKVLGYTGDFSWRTK"
     gene            30165..31445
                     /locus_tag="PLR13_05530"
     CDS             30165..31445
                     /locus_tag="PLR13_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18830.1"
                     /translation="MKKYSSVEKMAPVGFVLLGAGSVGGGVFHQSLLTRDVYCKVICD
                     KAMEKVMALEDPRRRFEIVSTEGELADALNAGKVAVCEDAQLAAIAPGVEVFFDASTA
                     VDSASRTIESAMCSGKHVVMMNAEADAVFGPWFWHLAQKYGVAYTSSDGDQPAVIARL
                     VEELRFYGLDFVMAGNIKGFLDRYTDPVKIIPEADKRFLDHKMCSSFTDGTKLCVEMS
                     IVANALGCSVVQPGMLGPKMNETIELFDHFPLSDLWTPGQAPLVDYVLGARPQGGVFA
                     VGYTDDAYQRRMLNWFPSETGPGPFYVFTRPYHLIHFETMRTVLEVARGHRSLMAPRF
                     GMRTEVICYAKKALEPGHLLDGPGGFASYGLIENSDSGLPAGFPIILSDGVKLRRAVS
                     RDQRVTLDDIDISTVSPDALESWRRACKIGTQNV"
     gene            complement(31592..32446)
                     /locus_tag="PLR13_05535"
     CDS             complement(31592..32446)
                     /locus_tag="PLR13_05535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQC18831.1"
                     /translation="MDKNDHKVSIITPVYCPSQYLIDLTEKMFLESLAGSSMKNNLEI
                     IIVDDASPKRNELKAMVEKTAKEKGLNIMLIRNETNQGIAKSQNRGVDQSKAPYLLLT
                     NNDIYAPKDAISRLLNLLRSNSQYGAVGPKLSFAFGHRIQEIDTGIILNDFTTDEFKK
                     IDAAAERLVKMHDGDITTVNSLVGCFLMTPRSVFEEAGGRNEIYGLGYHTESEFLIRV
                     RKKGFKVAVDQGTLVFHGDVGKRDFYGPSMKSVQRKATYNLLRNYAYFVFRNGWKELF
                     SLLNDYKK"
     gene            complement(32587..>32805)
                     /locus_tag="PLR13_05540"
     CDS             complement(32587..>32805)
                     /locus_tag="PLR13_05540"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HQC18832.1"
                     /translation="GKAVARNILLDGNTFRDSHSVEKEPFVAEVIGGLGIIIHRIKIT
                     YSYVHRTKEFEKQKDAQHFGALALSFTF"
BASE COUNT         8169 a         7650 c         8203 g         8583 t
ORIGIN      
        1 aagccggctt ccccgcgtgt ttttagatta ttcaacgcag gaaatttttt gaaatgtgga
       61 tatctccttg atcgggattc ttttctttaa cagctcttca ggaggcttat cttttaagca
      121 ataagattgc tccagataaa tatagatggg ggagagtttc gcatgcaatg tttttgattc
      181 aatcagcatt tcgctgaaaa cgtcacacac ccccgaatct tcctgaataa agactctgat
      241 ctccgggacg ggaatcgtat cctctttcaa accgtttacg atttcaatat agccgatgaa
      301 atttttatat tcgtctgtca aattctttgc cggattaaaa acgacgccaa cattttcaaa
      361 ttccggaacg gaaatattcc ctttatagga aacatgacag gcaacgcgtg cgtgagacga
      421 cggattttgt ttcccgaaaa aagttgtcaa tttatttttt tccgaaaccg taaaactgac
      481 gggtaggggc gaattgatca atttgtgaaa cgcattcaac atttcggttg tccatgtttt
      541 gggaagtttt tgaggttcgt ttgccggcat aattttaatc cttttttgtt agagattaat
      601 gtagctgcaa attcaataat gcattcccga aaaagaaaac ccattttctc tgtgcgagaa
      661 agtgggtttt ggattttccc ccgtatggtt catctaatcc actcatgcca tttgaaagtt
      721 ttatgagcac tagactactg agaaatcatt tcaacattta ttttattcat aacctttctt
      781 caagaatatt tcaatgaaat agtttttttt accgcgcagg gggatattaa atcgaaagct
      841 tgatgtttgc agcattattc aggacagtgt ttatctcaga tttggtgctg ccggcgctca
      901 ctgcctcaat ccaggaatgc gttcattctc cgccttgtgt gctccatcag ttcaggcagg
      961 tttccgtcgt gataatccgt gacgggaatc ggcttatgaa tgatcatcgt cgcaccgccg
     1021 ggggtcaaat ccaggctgcc tttcggcaga atgtttcggg taccgttgat ggtgacggga
     1081 agaatgggta tgccgaggtc aatggccatc ttaaaggcgc ctttcttaaa ttgtttcatc
     1141 ttcccgtcga cgcttcgcgt tccttccgga aaaaacaaaa cggaggttcc gccaacaatt
     1201 tttgccctgg cggctttgat ggtgtccacg gcacttttcg agtcggatct gtcgatgtaa
     1261 atgtgaccga taattttaca ggccaaacca agaaccggca cttttttcag ctctttcttc
     1321 atcacccatt tgaagtctat gcccagccag ccgtaaagca catagatatc atacatgctc
     1381 tggtgattgg aagtgatgac gtaagacgtg ccgggttcga tgttatccgc tccgacgacg
     1441 tgtacagtca tgggggtgca gaagctgttg attctggccc aaatcattcc aaaaattcca
     1501 cctgtgcgcg gtgagaacag cagcgctgtg acaatggcca aaacgccgca gatcagagtg
     1561 gtcgcaatca ataaaggcag aacaaagagc catttataag gctggtaact gatatgggcg
     1621 atccagtcct ttttctctgc cagggttttg ttcaagccgt cggaaaatct ttgccggttg
     1681 tccagatatc tctgccggta tttttcaaag aattcttttg tttcgcgttc caatctcata
     1741 aatcccccgg tggagattgc agcgtttttc tgatgatcaa agcttgtcct tcacttcgtg
     1801 ccagaataat tcgactcctt ctcgccatcc cttcaggtat ttttccgaaa agtcactcca
     1861 agcctcctgt gcttttttgc ccgttgccgc cacttgcgcc ttatatttct taaatatgtc
     1921 ggagaaataa tcgcctaact cttcatcctg gttgggattt tcgcaagcgg tccaggcgag
     1981 ggcatattga atttcacggt aatgggcggt tttgcaccat tcgaggccgt cctttttccc
     2041 ccattccgcg atgttgagct cgtaatcgac cttttccttt ttcaggcgat ccacgataga
     2101 tgaaaattcg atttcgttcc ggattttcga tgttaaattc tcttttttct gtatgatttt
     2161 ggaaacggca ttctgaaata ctctggaaaa attcagagac gatttccatg cctgcatttt
     2221 ttcataaagc tcgtccggaa cgctgatcgt tattttttgc gccataatac atactccaaa
     2281 atttaataca tatattttaa atagtacata tatgattaaa aaggatgtgt caactgcaag
     2341 ttgttgtgcg aatataatgc cgcaaccaat tcagaaagtt gaagcagttt acgctgatcg
     2401 atagaacaat cagctttcaa caattttgcg taaaagtggt aacgataaat ttgagggtaa
     2461 aatctaaatg gcgccagcag aaaacagatc aagcgacggc atgctgctgg ctgattgaaa
     2521 tgcaaaaaag ctaaattgtg tttttaacgg tatgaacccg aggcaacagc tgcgaaaaaa
     2581 atgcgcgccc gaacataggg gcgcgcatgc aaaaacgaat acgattattt ttttccgtag
     2641 ccgatggttt tagcggcttc tgcaatactt cccagaaccg agggatcgtc aatcgtagaa
     2701 ggaatcacat actccttgcc gtccacgatc ttgcgcatcg tgctgcgcag agtttttcct
     2761 gaacgcgttt tcggcagtgc cttgacgatg gccgcttcct tgaagcaggc cagcgcgcca
     2821 agttctgaac gaaccatttg aaccagttcc ctgacgatat cgtccggatc tcggtcgacg
     2881 ccggctttga gcacgacaaa gcctacggga acctggcctt tgagctgatc ttcggcgccg
     2941 aaaactgcgc attccgcgac atccttgtgc ttcgaaatga tttcttccat cgctccggta
     3001 gacaggcgat gccctgcgac gttgatgacg tcgtcgatgc gtcccatgat aaaaatataa
     3061 ccgtcctgat caaagcgtcc gccgtcgccg gtcacataat aaccgggtct ttccatgacg
     3121 tagtccagat aacgtttgtc atcgttccaa agcgtgggca gacagcccgg aggaagagga
     3181 agtttgataa ccacggaacc ttcttcgccg ttgggaagct ggttgccgtc cccgtccaga
     3241 atctgcacgt tgtaacccgg gacgggtttg gtcggtgagc ccgccttgat ggggaatctt
     3301 tcaatgccca tgcagttggc ggcaatcggc catccggttt ccgtctgcca ccagtgatcc
     3361 accacaggaa ttttcagcat atcgctggcc cagtgatagg tatcgggatc gagtcgttcg
     3421 cccgccagaa aaagatacct aaggcatttg atatcgtatt ttttcagata gtcgccgttg
     3481 gggtcttctt tcttgatggc gcggaacgct gtgggagccg taaatagcac agagacgcca
     3541 tgctgggaaa tgacgcgcca aaaagcacct ggatccggtg ttccgaccgg tttgccttcg
     3601 tagacgatgg ttgtgcagcc ataaagaagc ggtgcataaa taatgtaaga atggccgacc
     3661 acccagccga catcggaggc ggcccagaaa acatcgcccg gtttgacatc gtatatattt
     3721 ttcatagacc atttcagcgc cacggcatga ccgccgttat cgcgcatgac ccctttgggt
     3781 ttgccggttg ttcccgacgt atagagaatg tagaggggat cggtcgcggc aacggatacg
     3841 cagcccgccg ctttggcatt tttcatcacg tcgttaaagt ccagatcgcg tgccgaatcc
     3901 atgtccgcct tgacctgggg acgctggtag ataacgcatt tctgcggttt atgagccgca
     3961 attttaatag cctcatccag tagcggttta taaggaatgg tcttcttccc ttcgatgcca
     4021 caggatgcgg agatgataat tttcggcttt gcatcatcaa tgcgtatggc cagttcgttg
     4081 ggagcaaatc caccgaaaac aacggaatga accgcgccga tgcgtgcgca ggaaagcaca
     4141 gcaaccaggg cttccggaat catgggcatg tagataatga cggtatcgcc ttttttgacg
     4201 cccagggcac taatggctcc ggcaaatttg gatactttgt ccagcagttc tttgtaggta
     4261 attttctgca cggtattggt tacggggctg tcataaataa tggcgttttg ttccccccgt
     4321 cccgattcca cctggtaatc aagggcgtta tagcaggtgt tcaattcgcc gccggaaaac
     4381 cagcggtaaa aaggtttttt gctatcgtca agaaccttgt cccatttttt gttccagacg
     4441 acgtcttcgg ctgctttgcc ccagaaaatg tccggttgat tgattgattc agaataaact
     4501 cccgcatact ttgatttgtc agccatgatg agtcctcttt aaatgaatag atttgctatg
     4561 ggcataacag attgaaatcg aatttgtcaa agataaagat catttctttc gtgagacttg
     4621 aatcccgctt caacggacag atttccgttt gctgtgctca ggggataatt cgatcggcca
     4681 atgtccatac gcgatgcttt aaaattgaag cctgactaaa aaaagcgggg caacgatatt
     4741 tcgttgcccc gcctcattgg attggtcggc agaggatgcc cctctgtcgg agataaaaca
     4801 aaactgttta tttcatgttt ttaaaggagc cgtccacgat ttcgggttcg ggtttgaaaa
     4861 tattcagacc gaaacgggtg tgcagtttat tgagcgtatc tgccagttga gcgggttcca
     4921 tgccggcgcc aaaggcgaac ggaccggcaa acgctctcat gcaatggacc atgcccaggt
     4981 cgatatcggc agcggatgcc gcaatcttct ctttgtaaac acgcacggct tcgttgatct
     5041 gaatggccag aaaatccagc ggaccgaagt cctgagattc tacggacata tcgatcttgg
     5101 ccttgccgcc ttcccaggtg taaatgccct ggccgctctt catgccgagt ttcccgctgt
     5161 ccaacagagt ctgaagtgtc ttgccgggtt tgaattccgg agacagtgtt tcggaataat
     5221 atttgagagt gtgatagaaa acgtcaatac cgacgtagtc agcaagctcg aagggaccca
     5281 tgggcatacc ggccttggcc ttcatgacag catcaatcgt gtccactttg attttgcctt
     5341 catccagaat ggcgctgatc agagcctggt tgggtgctcc gatgcggttc acgataaagc
     5401 ccggggagtc cttgagcact ttgatgggaa cttttccgat tttcttggtc agttcgcaaa
     5461 gcaggtccac gttggcatca gaggtttgct tggcgtaaat cacttctacg cctttcatga
     5521 ccggcgcggg attgaagaaa tgcataccga gaatccgctc cgggttcttc ccggccgtgg
     5581 cgatttcgct aatgctcatg gtggaagtgt tggacgctat cagcgcgtca gccggagccg
     5641 cagccgatat ttcggcacac actgttttct tcagagacat gatttccgga acggcttcga
     5701 taatcacctg aacgtccttg actgcctcgg tattgcttaa agatgtggac aaattgccca
     5761 tgacttcggc ctttttctca gcagtcattt tacccttgcc gaccagagta tccagactct
     5821 tgttgaccgt ggccatacct ttgtcgagaa actcctgttt gatgtccacc atgacggcct
     5881 tgatgccggc ctgtgcgcat acctgggcaa tgccgttccc catagctcct gaaccaatta
     5941 ctgcaatttt cttgatatca tctaacttca ttaccgacct ccttcttctt aattgaatta
     6001 ataagaactt cctgtccaaa tttcattgtg attcttctta tgtttatata agaattttgg
     6061 cagcaattct ttttcgtgtg agcggagtat aggtaaagga gggcgaacct gtcaagggaa
     6121 aaaggattaa tattgtattt aatagggacc ttgcctgcag gggcaaaaaa caggaaaaag
     6181 gccattttgg cgaaggcgaa aagaaaagcc tgccttttaa aatgtttaga aaaggacttg
     6241 ttttgatgat tgcctaaaca ggaacaaatg tatatattaa agaacaaatg tcgttttggg
     6301 gttattcttc aaccgtttca gaatttaaaa aaggactgta cgtttaagga ggttcggtat
     6361 gaattctttg aaaaaggcta ttttcatcgt ttgtttcttc aacctcttgg gtttgtggtc
     6421 tttgtccctt tccggggttg ccattgcaaa ccaaaatgag caagaagcct ctcttgaaaa
     6481 atgcatcaag tcctgcgaag acagtaagca ggtttgttgg aatatggacc ctgaccggag
     6541 gctttgcgaa gcccaatttc agttgtgcat ggagaactgt aataaaaaat acaacgcgcc
     6601 tgtcgtcgaa gacgtaaaac cgccggagcc taaaaagaca cggacattgc attaagggtg
     6661 ttatgaattt tttgccctgc aggcgtcttt tgtttcgtta caggttcacg gcgaggtaaa
     6721 cttttattcg cagtgtgaaa tgttttatac atgccgcaag ttttttttcc ggtttcgcga
     6781 ccaggtattt tattaaaagc ataataaaga cagcccgttg tgttctattg acggttgccg
     6841 gttttctggc acaaattgtg cttttacatt atttactttg tcgttgttat tgtgcgggat
     6901 ttacgctaca ttcacgttaa aaaacgagga aatatttgtc tgcatgaaag atgataaaaa
     6961 tatagcggct gttttcccgg ggcagggttc gcaaagaccg ggtatgggaa aggatttcta
     7021 tgagcaaatt ccggtttgcc ggcagacttt tgaggaagct gccgatgcgt tgggctggaa
     7081 tatcatggat atgtgttttg gggaggatga aagattaaat ctgacagagt acacgcagcc
     7141 ctgcatcgtg acaacggaaa tctgcatgtt gcgcggattg aatgagcgct atggtttcgc
     7201 gccggattat ttcggcggtc attcgctggg cgagtttacg gcgctggtgg ctgcaggtgt
     7261 gctgcccctg ttcgatacgc tcaaaatcgt tcagatacgt ggacggttaa tgcaggatgc
     7321 tgtgccggtc ggtgtgggcg ggatggcggc ggtcatttcg gaaaacatcg atatgaacat
     7381 gctgaccaaa accatggagg gcctgaaggc cggcatcgcc aatatcaatt ccgccaacca
     7441 ggtggtcatc agcggagagc tgccggctat cgacgaagcg gaaaaacgtt ttacggaaaa
     7501 ttttccggaa ggtaaaagct ttcgttttat tcgtttgaat gtgagcgctc cattccacag
     7561 cacgttgatg aaaaccatcg agggccgttt tgcggaaacg ctggaaaaat tcggcagtca
     7621 tctgaatccc gtaaacgctc cccgtgtcac ctcgaatttt accggcggtt ttcatgcggg
     7681 cgataaggcc gacgtaaaga aaaaccttgt tcaccagatc agccatacgg tgaactggcg
     7741 caagaatatg caggctctgg cggaaaatac gcaggccatt tatgaaatcg gaccgggcag
     7801 accgctgcgc gatttcttta agacgatagg cgttacctgt cagtccatta ccggactcaa
     7861 cgcggcagaa aaagttttta aggcaacatg tcagtaaacc agccatcgcg cgaatgattt
     7921 tcgcaaagga gtaaatattt tgatacagga aaaagacata aaaaatattc ccctgaagat
     7981 tcaggacaat accttccgtg acggccatca gtcgatttac gcgaccagaa tgcggaccga
     8041 agatatgatt cctatcgccg aggagatgga tcagtgtggt ttccacgcga tggaagtctg
     8101 gggcggcgcg acatttgata ccatgcacag atttctggga gaagatccct gggcgcgtcc
     8161 ccgaatttta aaacagtata ttaagaaaac gccttttgcc atgctgatcc gcgggcagaa
     8221 tctggtcggt taccgccatt acgccgatga cgtcgtgcgc gctttcgtgg ataaggcctg
     8281 tgaaatcggc attgatgttt tccgctgttt tgatgcgctt aacgatttcc gcaatatgga
     8341 atcctgcgcg gaacgaatca aggcaaacgg caagattttt cagggagcgg tttgctattc
     8401 gctgacagat gtccgtctgg gcggtgacgt ttacaatctg gactattatg tccggaaagc
     8461 caaagcactg gaacagatgg gcgcgaatgc catttgcatc aaagacatgg gcggcattat
     8521 gtcgcctttc gatgcctatg atctggtaac ggccatcaaa aaagaaatta acctgccgtt
     8581 gcatctccat tcccactaca ccagcggcat ggcgtccatg acgtatttaa aagcgataga
     8641 agcgggtgtc gatgtggtag atacgtgcct ggcgccgttt gcgctgagaa cgtcgatgcc
     8701 ggccattgag ccgattgttg ccgccctcag aggcacatca cgcgatacag gcattgatct
     8761 gtacaaactg cttgcgctgg gcgaacactt ggaaaatgtg gccccgaaat acaacagcca
     8821 cctggctacg cataagcttt cgattatcga caacggcgtt ctcgctcatc agattcccgg
     8881 cggcatgatt tccaatctga ccggccaact caaagaaatg aacgccctgg acagactgcc
     8941 cgaaatttat aaggaagtcg tccagaccag aaaagacatg ggatcaccac ctctggtgac
     9001 gccggtgtcg cagattatcg gcgcgcaggc ggtgctcaat gttttgtttg gccgctacgt
     9061 aagagtttcc aatcagctca aagatctggc tctgggtctg tacggcaaaa cacccatgcc
     9121 gattgatgcg gcactgacaa aaaaaatact gaagaactat aagcgcggcc aggagccggt
     9181 atccggaagg cctgccgatt atctggagcc ggaaatcgaa gcggcgaaaa aggccatggg
     9241 ggacctggca cgcagcgacg aagatgtgct ggcctacatc ttatatccgg cgaccatgac
     9301 gaaatttctg caatcgaagt atggcatcga accgcccagg gcgacaggcg acgacaatgc
     9361 ccagaaaaaa ggcgcggccg cataaaaaat ttaaaccgaa ataaactcct tttttgaagc
     9421 gaaaaattct taaaaggagt tgttttataa gttcatctcc aaaaaaataa aataagtaaa
     9481 ggtgtgcttt atggatttca gcctcaacga gagccagtta tcctatctgg atactgttcg
     9541 caaatttgtg aagaatgaaa ttacgccgcg tattctggac atggaaaagg agcacgtttt
     9601 cccctgggag atcattaaca aggcctggaa aacggggctg atcaatttga gcatcccgga
     9661 atccgtccgc ggttatgaaa tggatctgtt tacgtccgcc ctggttgttg aggggctttc
     9721 ctacggcgat acaggcatct ctacgtcggc catgtgcaac gatctggcca acgtcgtcat
     9781 cggactgcac ggcacagacg agcagaaaaa tctattcctt aaaccgtttg tcgaaaagcc
     9841 gatcctggcc tctttttgtc tgacggagcc caatgccggt tcggataact ccatgatggc
     9901 tgcgttcatc agcaaacggg aagacggtaa gtatattctg aacggggcca aatgttttat
     9961 caccaacgcg tcttacgcat cgcaatttac tgttttgtgc aaaacaggca agccgaccgg
    10021 caatttcatg gcctgcgtca ttgtgccggt ggaacacgtc acttcatacg atatccccga
    10081 tatcggcaac gcaacccgtg aaatcagcgt tcccgccggc ggtaaaatca tcatcggtaa
    10141 acccgaggat aagctcggac aacggctttc caacaccgcc agcgtgacgt tcgaggacgt
    10201 tgtcgttttt ccgaatcaca ttatcggcga ccggcgtctg ggtttcaaat atatcattga
    10261 tgttctggat tatgcccgtc cgatggtggc agccatcggc ctggggcttg ccaaaagagc
    10321 gttcgatctg acggtggaat atacacggga acgcaagcag ttcggtcatc gcatttgtga
    10381 ccttcccgtg gcgagagaaa tgctgacgac gatgtggaaa aaagtcgagc tggccgagat
    10441 ggctttgttc aaggccattt tcaagattca ggaaaaagcc caagatgccg gcatctatgc
    10501 gtccctggcc aagaacaccg ccgcggaagc tgccgtcttt tgtgcgacgg aaggcctgca
    10561 tctgcacggc ggttatggtt ttacaaccga atatgaaatt tccaaactgg cgcgcgatgc
    10621 tcacattatc gatatctatg aaggtgtccg ggaagttcaa aatatgatta tcggacggga
    10681 gattatataa tgctttatct tcatgccatg gggcactttc atccggagaa tgtcatttcc
    10741 aatcagtttt tggaagaatt ggatatgggt acgtctaacg actggatcat ggaacgggtg
    10801 ggcattcaga accgccgcac ggttttgccg ctcgactata tccggaaaac caaaaacgtc
    10861 catccgctgc aatccttttc catccggctc tacaccaatg cgcagatgtc agccatggcg
    10921 gcacggatgg cacttcagcg cgcgggattg aaagcgaagg atatcggtct gattatttcc
    10981 ggttcatcat ccccggataa cattactccc gcggaagccg gagccgtggc tgcggaactg
    11041 gggatcgagg taccctgctt tgatttgaat tccgcctgtt ccaccttcgg catgcaggtt
    11101 aatttcttgt tgcgtatgaa gcctgaagaa atgccgcctt acgttcttct ggtcggtgcg
    11161 gaatccacga ccaaagtcat taactatacg gatcgtaatt catcagtgct tttcggcgat
    11221 ggcgctcatg ccgccgttgt ttcgtcgacc attcccgccc gtgccgcctt tatcagcggc
    11281 ggttataacg cccgtccttc gggatggaca aaagtcggtg ttaccggaga ctgggttttc
    11341 agtcaggacg gcaatgccgt tcagggtttt gccatcagaa caacgacgga atgtttaaaa
    11401 aaacttcagg atcaatacga ggctcaggcc aagcgcttta tcttcattgc gcaccaggcc
    11461 aattacatga tgctgaaaac cgtttgcgaa cgccggcaca tcaaaccgga acatcactgg
    11521 ttcaatgtgg atcagttcgg caatgtcggc tgctgcggcg ctcccgcggt tttgagtatg
    11581 aactgggata atatacggcc gggagataat attgccctgg tgattgtcgg cgccggactg
    11641 gcttgggcgt atgcgatgct caaggtggag gataaacaat gattgaaagg cccgttgcac
    11701 tggttaccgg cggagcaaaa ggcattggcg ccgcgtgctg ccgagcgctg gccgcggaag
    11761 ggtttcatgt cggtattcat tacaatcaaa gtaccgcaag cgctcaaacg ttacgggatc
    11821 aaattagaga aggtttttta attcaagccg atttatccga catcgggcag gtagacgcga
    11881 tgatcggtaa gcttaaggat accacgggac gtgtcgatgt tctggtcaac aatgccggtc
    11941 gctccgtcaa tgccgatatt atctccatga aagtggaaca gtttgacgag caacggacgt
    12001 tgacgcgcgg cgtctggtac ctgaccaaaa gaattttgcg gcaattcatg ttccggaatt
    12061 ccgccggccg catcattaat atttccagcg ttgtcggcca taccggcaac gctggacaga
    12121 ttccctacac gatggaaaaa gcggcgctcg atgccttcac cagatcgctg gcgcgggaac
    12181 tcaagggaag aaatattctg gtgaattccg tggctcccgg ttttatcgat acggagatga
    12241 cgaaggactt gcccgaagaa ataaaaaata aagccatgga agacattctc ctgggacgtg
    12301 taggccgtcc ggaggaaatt gccgaagtag tcgccttttt ggcaaaaaag ggtagttaca
    12361 tcaacgggtc ggtcatccac gtcaacggag gactttatgg aggctgatgc cgctctgaaa
    12421 caggaggtgc tttcgcttgt tcctcagcag ccgcctttcc gttttatcga tgaaattatc
    12481 agccttgacg atgagcagat tatcggggct taccgttttc gcgaagatga atatttttac
    12541 cgcgggcatt ttcccggcaa tcccatcacg ccgggtgtta ttctgattga aacgatggcc
    12601 caggttggcg tcgtggcctt tgggatatat cttttgtcgc gtcaaaagaa tatgcgactc
    12661 catgacatga aattgccgat cagtcttttt tctctggccg acggagtgga attcaaacag
    12721 attgtgaagc cgggtgaacg agttattgtc agagcgaaaa aaatttattt tcgtaaaatg
    12781 accattaaag ccgctgtgtc catggaaaag gaaaacggcg aaatgatctg cagcggggaa
    12841 ttatcaggag tgggagtgga acaatgaaaa aacgaattgt cattacagga atgggggtgg
    12901 cggcgcccaa cgcgcacggc ttggataatt ttgaaaaggc tcttcgcaat ggcgtttccg
    12961 gtattcgctt catcccccgg ttacaggaat tgaatttcgg ctgccagata gccggcgtcc
    13021 ctgaatattt cgatgaaata cggtcaagct atttcgaccg ggaaaaactt ttgtccatca
    13081 acgacaatat cggttatgcc tcggtgtcgg cggtagatgc ctggacggat gcgggatttg
    13141 ccctgaagga tgacgatgac gataccgtag attgggatac gggagtgatt gccggttccg
    13201 gaattggcgg catggatacg attgccaata ccatcgttcc tttaattaat gagggtaagg
    13261 taagaagact gggcagtcgt gttgtcgaac aggtgatggg aagcggaacc agtgcgcgta
    13321 tttccggcct tctgggagtc ggcaaccagg taacctcgaa ttcttcggcc tgctcgacag
    13381 gtaatgaggc gattattgac gcgatgggga gaatcagaac gggactggcc aaacgcatga
    13441 tcgccggcgg ttccgaaggc gcgacgcctt acatctgggg tgggttcgac gccatgcgcg
    13501 tgctgtgtag aaaatttaat gacaaccccg aggccggctc ccgtccgctc agtgcttcag
    13561 cggccggttt tgtccctggc gcaggcggcg ccatgcttgt tctcgaagag ctggaaacgg
    13621 cgctgggccg gggcgcccgg atctacgctg aaatcctcgg cggttatgtg aactgcggcg
    13681 gccagcggat gggcggctcc atgaccgcgc ccaatcccga aggcgtcaaa agatgtattc
    13741 gcggcgctgt cggcgatgcc ggcattgatc cggcacaggt ggacgccatc aacgggcatt
    13801 tgaccgcgac gtacgccgat ccgtatgagg tgaagaactg ggcagaagcg ctggagcgga
    13861 caccggaaaa ctttccctac attcagtcca cgaaatcatt gatcggtcat tgtctggggg
    13921 cggcgggcgc cattgaatgc gtggccgtcg ttctggaact gttcaaagaa tttttgcatc
    13981 cctccgtcaa cagcggggat gttcaccccg aaatcgaaaa attcgcgccg aaaataccgc
    14041 aaaaatgtct ggacgtacct gaaataaaaa tcatcgccaa agccggtttc ggtttcggcg
    14101 atgtcaacag ctgtctcata ttcaagaaat gggaagggaa ataatcataa aaattgaagg
    14161 agagttttat atggacgaca agaagatttt cgaagagatg atcaatattt tgaggcctta
    14221 tgccaagaat caggatcgtt tggcgacggc aacactggaa acgcacatcc tgaacgactt
    14281 gaaagtcaat tcggcacgcc tggtagacgt cattattaaa tgcgaagacg tttacggaat
    14341 cagcgtggat gatgatgagg cggataaaat acgcaccatc ggtgacgccg ttagaatcat
    14401 caagcaaaaa cttgtttaga caatgacgag aaagtctctg atccttttaa atatgcagaa
    14461 attcattggg cgcatggcca ttttcatcct tgcgccgctg tatttctttg ccgcaaaaat
    14521 gcttttttat cgtgtcagag atttaaaaaa aacaagaagt ttatgcgccg cagaatttgc
    14581 aaagcacaaa gggggatgga ttatctgttc caatcatctg accatgatag attcttttat
    14641 cttaagttac gcaatgttca gcttgcctca gcacatcatc cattataaaa aaatgccttg
    14701 gaatctgccg gaatggacca attacaaaga caatatcttg ctgacgattt tgtgctatct
    14761 ctccaaatgt attcctatta atcgcggtgg cgcccgggaa aaaatgaaag agtctctgga
    14821 aaaatgcatg tacctgctga aaagcggaga aaccatcatg atcttcccgg aaggcaagcg
    14881 ttcccgcacc ggaagggtcg accaagaagg tttttcttac ggcgtgggac gatttatcaa
    14941 acatctggaa gactgcaacg tgatgtgtat atatcttcgg ggagaccaac aggaccatta
    15001 cggtgtcatt cccgcctggg gcgaaaaatt ttcagttcaa atggaagttt tcaagccgca
    15061 tccggtagag ggcagtgatc ttcgggtaca gcgggaatac gcccggcaga ttgttgaaaa
    15121 actcgcagcc atggaggagg gatattttgc cctgtgtcgg aaacgatgtg gtggatttga
    15181 aggatgcggc gaactcggga aaaagcaggg attcgcgctt tctaaaaaaa atcctcacag
    15241 atgctgaaat tgaatatgta acccacgcgg atcatcccga cagcgccctc tggttgctct
    15301 gggcctgcaa ggaaaccgcc tataaagtca tcaaaaaaat tttgccggac atcgtttttg
    15361 ccccaaggca atggcaggtt aacgttcata atattaaatc agaatatgcg gaaggggagg
    15421 ttggcatacc gggaaaagac ggcgtcttta tccgcctttt cgcgggggcc gactatattc
    15481 attgcgctgg ttccgatgat aaagcgacgc tggataactt aatctggagc gttgaacctc
    15541 tgccggatga agaaacctgt ccatcgcttt tttcccgccg ctgtcttgct cagagcttag
    15601 ccgggcgctt ttcactggac ttccatcaaa taaaaataca aagaaagaga cggaacggcg
    15661 aacttcaacc gccatacgtt tatgtcgatg ggcggcaaac ggacacggag atcagtttaa
    15721 gccacgacgg ccgattcgtt gcctatgcat ttgggagtac cgcccaaaga aaatgacatg
    15781 aggaccgggt ttcatgatca gagagtctca catccccatt cgctgagttc aggggctagt
    15841 ttctaattcc gataaatcgg gattggataa ttcgacgtac aaatttcatt gcacgttgct
    15901 tatgaaattt gagtctcatt acgactaatt cttcaaactt cgctgtgctc gttttaccaa
    15961 ggcgtgatat gcttagtata gttcgactta cgcctcaaac ttcattgcat ttgttttcgg
    16021 cttgtctcac tatcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcttc
    16141 aaacttcgct gtgctcgttt tcagagggtc tcactaaaaa aggcttgttt ttttgatttc
    16201 ttttgggata ataagtgatc ggacatatca tgatttttcg gttctcgaaa gtgtaattaa
    16261 cccggaaacg attacccaaa agtaacggat tgttatgaac gaaaaaagaa aagccagccg
    16321 cctgatggaa gaaagtgagg taacaatcac cctgattgcc ggtggcaaaa agtttttcag
    16381 ggaaaaagtc atttataaca gcagcaagga tatttcggaa tcaggcgcca gggttcactc
    16441 cagcattttt ttgccgatcg acactctcct caagatagat tttaaattaa aaaaccttca
    16501 acacatgatt accgccatgg gtaaggtgaa gtggatcaaa attatctgtg agggcgaagc
    16561 atatgatgcc ggggtggaat tcgtgaacac gccgacggaa gccatcagaa aaatagcgga
    16621 ttacattgca tggcggcaaa agcagaaaac cacaaaacgt cattgaccct attggctccc
    16681 gttgtttgaa tacgttccgg aaggcggtct tgccgtgaaa gtgtaatgtg cggcatcggt
    16741 ttggtgtgaa aacggcagat ggttgattcc ggcatcttga aatgtccgga agaagagaaa
    16801 tcatcatcag gagggatcat ggaacagaat atgggtaccg ttgacaaagt gatcagaacg
    16861 attgtggcga ttctcattat tgtcgcctat cttatgggta tgctgccggg aatcgcgggg
    16921 ctgctgctca tcatcagcgg atcgcttctc tccagtgtgg taagcggata ctgcccgctt
    16981 tatacaaaga ttggagtgaa cacgaaccgg taatccgcct gtctgcaatc gattgcgcaa
    17041 gctgttttgc cgggtatttt cttgagccga gaccttccct gtgataagag tcccgatgaa
    17101 tcccatatgg aataaaaggt gataatatga acattgccgt tttaaacggt tcgccaaaag
    17161 gtgacgtcag cgtgaccatg cagtatgtcc atttccttca gcagagattt ccggaaacgg
    17221 aatttagaat ttttgatgtg gcattgaaaa ttaaaaaact ggaaaacgac gaccgggcgc
    17281 ttcaggaggt cattgcgaac gtggcggatg cggatgctgt gctctgggcg tttccccttt
    17341 attttcttct ggtctgttcg cagtataaaa gattcatcga attgattttt gaaaaaaatg
    17401 ccgtttccgc ttttcacggc aagccggcgg cctgtctgac aacctccatc aatttcttcg
    17461 actatacggc ccataattat attcatgccg tcagcgacga tctgggaatg aattatttcg
    17521 gttcctattc agcggaaatg aatgacctgc tgaaaaccaa ggagcgccgc cgcctgaaaa
    17581 cgttcggtga attgtttcta aaagcaatca gagaaaaaac gccggcaatc agaagctaca
    17641 agccgctggt cgctccggaa atcgtttttg acaccgaagc gattcgccct gtggatgcgg
    17701 ccggtaaaaa agttttgatt ctggcagatg atcttgcggc aaattccaat ctccggaaga
    17761 tggtggagcg attgcagaaa accttttctc ctgccgccga actggtgcaa ctcaaggacg
    17821 ttgatatcaa aggcggatgc ctcggctgta taaaatgcgg tttcaataat gtttgtgttt
    17881 acgagggcaa ggacggttac atcgattttt tcaatcacaa aattaagccg gccgatattg
    17941 tgtttctggc cggcgccatt catgaccgtt atctgtcttc gctgtggaaa tgcttttttg
    18001 accgcagttt ttttaacggg catacaccgt ttctgaaaga caagcagatc ggatttgtca
    18061 tttccggtcc gttcggacag attcataatt tgcgtgaaat atttgatgcg accacgaaat
    18121 ttcaactggc cggtcttgcc ggctttgtca gcgatgacac gggcggaaat ccggaaatca
    18181 acgcagcctt gcagactctg gccgtccagg cggctgagca gagccggctc ggttatattc
    18241 aaccgccgac attttatcag gttgccggcg cgaaattatt ccgggatgtc atttacggca
    18301 ggttgcgccc tgtttttcag gccgatcacc ggtactacaa ggaacacggg atgtacaatt
    18361 ttcctcaaaa atattacaaa tggcgaatga tcaacagtac gctggttttc ctaacaaaaa
    18421 ttccggcgtt tcgccgcgag ttcttcaaaa gaataaaaaa ggaaatgata aaacccgcag
    18481 ccggcgcgtt gaagaaaaca gaatgatggt tgttgcatct gaaaagcata tatttcaata
    18541 aaactctgga ggaaacatga atcatcagga tgtcgtggcg ttcgtgacgg gaggcgcttc
    18601 ggggctgggc gaggccagtg ttctggaact gattaaaaaa ggcgctgccg ttgttatcgc
    18661 cgatgttgat acggaaaaag gcgaaagcct ggcggcgggt attggcagca aagctttttt
    18721 tgtgaaagcc gatgtcacca gcgaaacaga gatcaggcag gccatagaaa aagctgttga
    18781 gattttcggg aaaataaatg ttgtggtgaa ctgtgccggc attgtcagtc cggggaaact
    18841 ggtcggccga aaagggcctc tgcccctgga agcgtttaac aaggttttgc aaattaatct
    18901 ggtcgggacg ctcaatgtca tcaggctggc ggtggagcag atgatgaaaa atactcccgg
    18961 tgaagaagga gaaaaaggcg tgatcatcaa ctctgcgtcg atcgcggcct ttgatgggca
    19021 ggtggggcag gtctcctaca gcgcatccaa ggcagggatc gtgggtatga cgttgcctat
    19081 cgcacgggaa atttccgaat acggattaag gattgttacc gtggcaccgg gaatttttga
    19141 tacgccgatg atggccggat tgcccacgac ggtcaaagac gatatggccc aaatggttcc
    19201 gtttccaaaa cgtctgggaa aaccggtgga gtttgcccgc atggtgctgc atatcatcga
    19261 aaacacgatg cttaacggtt gttgcatcag gctggacggg gcgctgcgca tgccacccaa
    19321 atgatcagga tagagcacat agaagataat ttttatggcc gggcagaaaa cgacccgaag
    19381 gcaaaaagat atttccaggc ttctgcgcga aaaaaagtgc agtcctttac ggcgcgccgg
    19441 aaagaagaat ccgttttcgt ccaagaaacg gaacagataa aatacgattt ttgaaattca
    19501 ataagattta tcagccgggt ttgcaggata agaagaatgt catgatttct tctctccatg
    19561 cgcgccatcg tgcctttgct ttctgatttg ttcttgatcg gaaagaatct gtaggacaga
    19621 cacttacatc tcgtaggata aaatcccgct tttaattagg actcaagccg ataccgcaac
    19681 ccttgcaaaa ggtgtattta gtaatcgctt ttcagggggt atatgtattg catcagccat
    19741 gtaatacgcc ccaccttgcg gccgttgaat catgagcgtt tttttctaac aggaacgagg
    19801 ccaaagcctg gggcgtaccc ctcctgaaaa gttgagaaga ccgggttaaa acgaggtcaa
    19861 aaaaaggaag acgatacgga tttaaaaatg ctttttaggc ggaagcgaaa ggcatgaaaa
    19921 ataatttaga taaaggagaa taatatggct gaagcaaacg aacaagattt ttatgacaag
    19981 gtatttcccg tacctgacag agtaagagaa aaatcttaca tcaagagcag agcggaatac
    20041 gacaaactgt acaaagaatc gatcaccgat cccaatgcat tttgggccaa aatggccaat
    20101 gaaagactga cttggtttaa accgtttgac aaaaagaaag tgtcggactg gtccttcgac
    20161 gcgaaagatg ttcattctaa atggtttgaa ggcggcaaga tcaatgtcag ctacaactgt
    20221 ctggaccgtc atctggcaac caagaaaaac aaagccgcga tcattttcga aggcaacgat
    20281 cccaacgact ggaaagtata cacctattac gacatgtatc gcgaagtcaa caaattcgcc
    20341 aatgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacat ctggtatgaa
    20461 aaactgatgg ctgacgccaa accttactgc aagccggaag agatggacgc ggaagatcct
    20521 ctgttcatcc tttatacctc cggttccacc ggacaaccca agggcgtagt gcatacccag
    20581 gccggttatc tgctttatgc atcgatgacc cagcagctcg cttttgatgt gcgcgaagat
    20641 gatacttact ggtgcaccgc cgacatcggc tgggtcaccg gtcacagcta cattgtttac
    20701 ggcccgttgt gcaacggtca ctccagcatt cttttcgagg gtgttcccaa ttatccggac
    20761 ttcggccggt tctgggctgt cgttgaaaaa tacaatgtga ataacttcta caccgcaccc
    20821 accgcgatcc gctccatcgc caaggaaggc gacaagtggg tcgatatgca tgatatttct
    20881 tctctgcgcg ttctgggttc ggtcggtgaa ccactcaacc ccgaagcctg gtggtggtac
    20941 tacaataaga tcggcggcgg ccgctgcacg atttccgata cctggtggca gacggagaca
    21001 ggcggacaca tgatcctgcc tctgcccggc gcgattgata tcaaaccggc caaggccatg
    21061 gttcctttct tcggcgttgt tccggtattg atggatgaag agggaaaaga aatcaaaggc
    21121 gtcggcaaag gcgcgctctg tattaaaaat tcctggcccg gcatcgcccg cggtttgtgg
    21181 gatgacaaag agggtcggtt cgttgagaat tacttctccc agtttcccgg ccattacttc
    21241 tccggcgacg gcgccgaacg cgatgccgat ggcgattaca agatcaccgg ccgtgtcgat
    21301 gacgttatca atgtttccgg tcacagattg ggcaccgcgg aagttgaagc agcgctcacg
    21361 tctcacccgg atgtggctga agcagcggtt gtcggtttcc cgcatgacat caaaggccag
    21421 gggatctttg cttatgtcac gctgaacaat aatgtggcca agacggatga cctcaaaaaa
    21481 gccctggtcg cacatgtccg caaggtcgtc ggtccgatcg cgtcacctga tcagattcag
    21541 tgggcggatg gtcttcccaa aacgagatcc ggcaagatta tgcgccgtgt tttgagaaac
    21601 gttgccgcca agacattcgg tgatttcggg gatacgtcaa ccctggccga tccggcagtc
    21661 gtggatgatt tggtgaacaa tagaaagaaa ctcggttaac atttttattt gcgcgaaaaa
    21721 tttctgcctt tttcgcgcaa aatttccttc ctaaaaatgc cgtttcccga tcttttggga
    21781 aacggcattt tcttgatttc ggacttccgg cggggatgcc ttccttacgg ccgcatcaat
    21841 tccttgtgga ttctccaacc gttgtctatc ttttttaaat gcaatatttt tttgccggaa
    21901 ctttttatca aagaagaact gtattgctgg ataaagagaa ctttcgcgct gtcgccgttt
    21961 aaatggattc ttacatcatt tatgctgatg gcaatatctt tactgttttt gaatatattg
    22021 gctttatgag taatccaggc attcaaatcc ttctctttcg actggaaatc cggcgcataa
    22081 cagctgcggt aggcttccat atcgcctgat ttccagctgt tgatccattt atcgagcata
    22141 ttttttatga cggtttcctc cgagacaggt tccgtggcgg cgggagcttc tgcaggcaaa
    22201 ttttccgccg gcacgccgct ttctaccgct ggtttctgta gtatctcgtt attttttatg
    22261 gctgtcttgg atagttttgt ctcaggatag agcagattat tcaaagacgg gataacgcca
    22321 aacgcctgaa gcaggaccgc ggccagcaca aggacaaaaa agacgaagat gggtagtgcg
    22381 atccattttt tattccttgc gcgaggttct cgtttgattt tttcagtgtc tttctgtccg
    22441 gactgatccc ggggaattgt atcatctttt gtgatttcta tcgcagcggg atcgatcggg
    22501 gcaatctctg gtgttttttc gatattaaca ttgccggaat caacaggaga aagtttttct
    22561 ttaatgtagt ccaccttgtg cgattttaat ttccatgaga tgtaatcctg aagtttgccg
    22621 atggcttcat tgggcgggcc tgaaaattcc acaccggctt gatacgactc atcgtcaatc
    22681 agcaccttca cccatttgac ttttccgatg gcattgatct gctgccgcaa ccctttgggc
    22741 gtaaactcca gttcgataag cgtatccacc ggcatcagaa tattggtctg aagtttggcg
    22801 ccgcccgctg agatgtcctt ggtgaaattt tcaatgattt tttctttcgg aatattattc
    22861 tcttcggaaa aaacggttat ggtgacctcg ttttcctctt tgatcctgag cgattttcgt
    22921 ctttcttcca ttcattcact cctttgaatg ccggatttta cttttatccc gaatttgatg
    22981 acgtatatct tcaatatagt tgaaatcatt tttgtctgtc aataaaagat tgatggcaca
    23041 atgtttcatg cctaagggga tgtgggggag gaaaacagca tcggggcggc acggtaaagg
    23101 tcattattcc cgttccgtta tgtgtatgag ctgcaaacta tggggtgaat ttagactaaa
    23161 tttaacgtca tggaaaccat attttcgtct ttggaatcga gtacaaaatc gcaatggcgg
    23221 aaaattctca gcatggcgta attttcctga agcgtttccg cacggatggt cttgtaccct
    23281 tctttccggg ccaattcaat gcactgctcg gagagagtat gccccaggga tttgttttgc
    23341 cagttgtcgg agacgatgat ggcatattcc gcttcgcctt cacagcgctt gtttttgatc
    23401 agacgcgcaa tgccgagaaa tttgtacttg tcgccgtcct tcatctccgc gacgacggcg
    23461 atttcctctt cgggattcac attgcagaaa cgctgagcgc gttcgggtgt ggcggaaaag
    23521 ggcgacagga atctcagcca cagggacttt tccgaacaat tgttgacaaa atcggtccag
    23581 aggccgatgt ccttggggtt gatcaggcgc agccttaccg cgcggttgtc cacggaaagt
    23641 gaatttctgg taatgtgacg ggcgatggaa tcggcaattg cactctggcg tctgacaccg
    23701 taatggtaca tctggctgag cacctccgca gccatttccg ctgtggaata aacgggaata
    23761 ccggcctgct ccatcttgac cacatcatcg atcatgaatt ccttgcaggc cacgcagcag
    23821 agaaccggct tctccttgta atcgatgttt ttcatgacat ccacaaaaga gccgagtatc
    23881 tccccctgaa tgataacgat aaaaccgtgg acatcgccgc tttccacgcc gatgtcgatc
    23941 aggtccttca cctgccgggg cgtcatggaa aacgaacagt caatgggatt gccgatactg
    24001 gcataatcgg gcatgacctg cttgagacgt ttctgaaaat acggttccag agcggccaga
    24061 cgcatgttgg aaaggtacag catgtccgtc gcggcaacgc ccatcgaacc ggtataagta
    24121 ataacgagga cgccgttccc cttcggtacc ggctgtttgg agaaagcgcg ggtcagtgcg
    24181 aataaatgct cgttatcgcg ggcacgaatg atgccgctct gcttgaacgc ggcgctgttg
    24241 atctcgtcgt tgccggcgag cgaggctgta tgggaagaca cggctttttt accggcttca
    24301 gtccggcccg atttcaaaat gatcaccggc ttgcgcacgg ccgcccgtga agccacatcg
    24361 acaaaacgtt tgccgctacg gatgtcttcc atgtacatga caatcacgga aatgttatca
    24421 tcatcactca gatattccag aatatctgtt tcgttgatat ccatcttgtt gccgatagtc
    24481 gcgacaatgc cgaaatccag tacgttgctc aatcctgtca gaattccggc cgcgtaaacg
    24541 ccggcctggg caaccatccc gacatttcct ttgtgcaaat cggaaagcag cccgatggac
    24601 tgcaccatgt tgtgatgcgt gttgatgacg ccggaacagt tcggtcccag aacccggcat
    24661 ccgtgggcgc ggatgatttt ctcgattctc tcctgcgcct gtcttccctc ctcgccggtt
    24721 tcggcgaatc ctgccgcctc caccacgacg tactttacac cctttgagca gcaatcggaa
    24781 atggccgcct ccaccgcctt ggccggaaca atgacgatgg ccatgtccac cggatcgggc
    24841 acatccagaa tcgatttgta gcatttcacg cccttgatgg aatctttttt cggattgatc
    24901 ggataaagca taccggggaa atcatgattg atgagattcc agaacacgtt atagcccagt
    24961 ttccctttga cgtcggatgc gccgatcacg gcgatgctct tcggataaaa gaaatcccgc
    25021 aggctttccc ggcctgtcgc ggtaacattt tccccggggg atgtttcctt gacaaacatt
    25081 cgggcgtcga cggtaatata gccgtcagga tataaaaaca gcggattaag gtccagttcg
    25141 ctgatctccg gattttcgat gaccagttgc gatattttca tcagcaggtt gctgagggag
    25201 tccagatcga ctgcctggcc gcgatagcct tccagaatgg cggccccccg aatttccgcg
    25261 atcatttcgg aagcatcttt ttccgtgatc ggcagaatcc ggaaagatac atcgcgcagc
    25321 acttctacaa atacgccgcc gaggccgaac atgatcagcg gaccgaagct gggatcgcgc
    25381 gtcacgccga tgatggcttc gatgcccggc ttggccattt tctgaaccgt aacgccttcg
    25441 atatgctgat acttgaattt ttcaagaatg tcctgatacg ctgtgcgcac atctcccgcg
    25501 gaatgcaggt tcagtttgac gccgcccgcg tcggttttgt gaactacatc gggtgaaaca
    25561 attttcagaa caacggggta gcctatcttc tcgctcatgg ccagcacttc atcttcggaa
    25621 cgcgccacca gaaagcccgt ggtccggatg ccgatgcctt cgaggatttc cttggcttca
    25681 tgttccatca gataactgcg gttatccctg acggcctgat gaatggtttc agccagttca
    25741 tctttcagaa gcgctcttgc gaaagatgaa tcactctgaa catttttttt tgttgattgc
    25801 gctttttctg atgtcgaaaa ttcctgattc atgcccccgc ctccctgcaa ttatccgacg
    25861 ttttctcttt acggtacacg ttaatgccaa acttgaacat ggtaacggat tagcatgaag
    25921 attgaaaagg taatatcggc ggaaagcttg tttgtttgtc ggaacagtgc gcaatcgcct
    25981 gacggatttt gtccgacaaa cgcgcagcac gggcatggaa aaaagcagaa acgtaaaaac
    26041 ctaaccgatt aatttgttgt gaaccgcata cagggtgagc tccgcgtttt ttttcatgcc
    26101 cattttggac atgacgcgcg tccggtaggt actgatagtt ttaacactca ggcataattc
    26161 gtcggcgata tcggaaacgg actttccttc cgccagcatg agcataacct ggtgctcgcg
    26221 gttggacaga cgttcatgtg gcaatttttc cgtgtcgatt tccagctcgt cggccagttt
    26281 ttcggccagc gaggacgtga catattttcc gcctcccgcc accttgcgga tggcgccgat
    26341 caattcctgc ggggcgctgg ccttggtcag gtagcccgag gctccggcac gcagagcccg
    26401 aaccgcgtat tgttcctccg gatgcatgct tagaatcaaa acggccagtt tgggatgctg
    26461 ggctttaata tcttccagca cttccagtcc gctgcggccc ggcatggaaa tatccagcag
    26521 aaccacgtcg tagttgttgt cggtgacttt tttcagggtt tcaggaccgt tttgagcctc
    26581 gtcttgaacg gtcatgtccg tgacgtccgc cagaatttgt ttgacaccct gacgcaccac
    26641 ggcgtgatca tccgcaacca gtatacgtat catgacagtt tttctccttt ttccaatgga
    26701 attaaaaccc ttaccgttgt tccggaaggt ttggcgttgc ggacatgaac ggtgccgtcc
    26761 cagagatgag cgcgttcgcg catgccgatg atgccgaaag accgggaatc gtcaatttgc
    26821 cattgcgtaa tgccgatccc gttgtccgta acttccagac acaattcctt tcctctttcc
    26881 gaaagggcaa ctttgcattg tgtcgccttg gaatgacggg cgatattggt gagtgtttcc
    26941 tgaaaaatac gaaagatgga ggttgccaga tccttgctga tggtctccat attgccggtg
    27001 atatacgcct gacagcgaat cccggatcgt ttctgaaaat cgccagcctg ccattcgatc
    27061 gctgccggta gtcccagatc atcgagcaga ctcgggcgca gttccgtggt gatcgtgtgc
    27121 acactttcaa tggtagcgtc aacaagttcc gacatactct ttgttttctc ccggatacta
    27181 ttgctgctgg tcggcaaacg ggttttcaac cacgataaat ccatctgtaa cgccgtcagc
    27241 gactgcccca attcatcatg aattttgcgc gccatgcgcg tgctttcttc ttcacgcacc
    27301 gattgaagat gcgcggaaag atttctcaag tcctgccgtg agcgttccag atcttcctgt
    27361 gctttctttt gtgcggtaat gtcttcaaag gtcaccacga tacgtttttc gcgcagtttt
    27421 tcaccgatcc gcgaggcttt catccggcag ataatttccc ggccgtcctt gcaccggcag
    27481 gggaattcgg tttcatatgt gcgttgtttt tccagcgtgg aataaaacag gtcaccgatt
    27541 gtgtcggctt cttcttcatt gcggtaaatc atccggatgc ttttgccgat gagttcctcc
    27601 cggtgccagc caaaaatgat ttttaccgcg tgattggcaa aattgatccg gcgttcatga
    27661 agtcccacga ccgcctgggg aatggcatcc aggatggacg attccagcgc ttccagctct
    27721 tccaggcgcc gaccggcttc tttccgttcg gtgacatcca tggaatttcc caggatggcc
    27781 cgtcctccct tgaatttaat ggacatgacc gtttccataa tccacttgat ccggccgtct
    27841 ctggtgacga tacggaattc gtaaggagaa aagcgctgtt ccttgagcat catgacggcg
    27901 cttttcgctg ttttccggcg atcctcgggg aggatgaggg aaatcggatc catcttgagc
    27961 atttcttctt cggtataacc ggcgtagtcg cgaaaatgtg aattgatgaa tctaaaacgg
    28021 ccgtcctgca tgatgtaaaa tccgacctga gagctttggg cgaggaatcg ataaagatct
    28081 tctccggacg tgccggatag ggacgatgcg tcggctgaaa ccagttgtgc ctgaagactg
    28141 ctcaccagtt cgatcagctg ttttttggac atcttttccg gattcattga gccacccgat
    28201 tctattctca gccatgcgaa aattatttaa tcataaaaca catcaaaaat aaatatcttt
    28261 gatggccggt ttttccagaa cagatggcgg ctgtatctat gagacgcttt tttcaggatg
    28321 tcggttcaaa aaaatcgaaa taacccgtga agagaaccgg gggatggaaa acaacttgaa
    28381 agcaatatca aaaatcgatt tgacgtcact tggtccgcca ggaaaaatcg ccggtatatc
    28441 ccaagacttt ccgtctggtg tatgcgattc cgctccagta cattttcctg aaaaaaggta
    28501 cggtaaaggt gttaagggtc acgacaaacg gtgtgacaat gggaagcaga acgatccaga
    28561 aatttacttg gattcttatt tccggttgca gtaagcggcc ggctatggct ccttttgact
    28621 tcatgatcca gaaaatcaat ttggcgctta atctcggttc tgcgtcgatg acggttttca
    28681 gcgcggagat tcccatgaag acgtcaatga caatggataa taacgccagc agacaccagg
    28741 gataccacca ggctatgacc agcgcgcata aaagcatcgg cagtctgatg ccgatattgc
    28801 acagcaaaac ccatatgccc ctggtcgtcc accaggtctg caagacctgc cggttggtaa
    28861 acgttatgac attggccaga ttttcctttt tttccggagg atgtctgaga acgatacagt
    28921 caggtacgaa cagagactcg tatccctgtt tccgcaaggc attatatata ttcatgtcgt
    28981 cggtggtgat ggaacttccc ccctgtccat tgatctgtcg cgccaggttt gcattatcaa
    29041 atactttttt gggaatggcc agtccgcctc cccatatata gcggcacttt cccacaagaa
    29101 acatggcaga aatcaaaatc cataaaactt gaaccagaga ggccagccct ttccccggaa
    29161 ggtaaattct tgcggacgta acaaccccca gcctggaatt ggcgtcagtg agaggagcaa
    29221 cgagacagct tagccagtca ctgaaaggac gggcatcggc atcaataata gcataaactt
    29281 ctgtttgcgg agaggcggct ttaatcgcag ttaataaatt attgttcttc tgacaccggt
    29341 gaatcccttt tgttgtcgcc agttcaacca cgtcttcggc gatggctatt tttacatttt
    29401 tatatttttg taaatagtgt tgtagctgcg gcagggaagc atcgtctttt gtcgtgacaa
    29461 aaataacttc ccagacgccg ggataatttt gtcccaggat agcctcgaca ttttccgggt
    29521 cgattttccc ccgttgcgga gagatcaccg tcagcgccgg atacgttgcc ggcgtctttc
    29581 tggtccagtg cttgatatga gagcggaaga taaaaacaga gagtaatccg atcagtgagc
    29641 tggcaatcgc cagagatgcg caaaaatatc caatgaattc aatcacagaa cgtccacatc
    29701 cattcagctc atggattgca gcgaggagaa agagtaattc aatatcgtcc ggtgaaatga
    29761 aaaaaccttt tcgccgtaag acggccttca tcctgcatga tgtctatatc cattttacta
    29821 attttaaaaa agtactgcgg gaacataatc gatgctgacc tgtctgtcaa ggtaatattc
    29881 attggaattt taaaaggtca ttgacatccg gatacggtac cgaagagccc catcggagca
    29941 agggtgacga agctttctgc cggggtgatg gttgcatccg gatctgtctg tgactttaaa
    30001 aattgatcag gaaccgtttt tatttcaaac atgatgcagc cttgagggca ttgggaaaaa
    30061 tattttacat cccgcggcag taagattggt aatccattga cgggcagccg caattcaagt
    30121 atattttttc cggggaaaga catttctttt ttttgaggtc ttctatgaaa aaatattcaa
    30181 gcgtcgaaaa gatggcccct gtcggttttg ttcttctggg agccggttcg gttggcggag
    30241 gggttttcca tcaatctttg ctgacccggg acgtgtattg caaagtcatt tgcgataagg
    30301 cgatggagaa ggtgatggct ttggaggatc ctcgacggcg cttcgagatc gtttcaaccg
    30361 aaggagaact tgccgatgct ttaaacgcgg gaaaggttgc cgtttgcgag gatgctcaac
    30421 tggcggccat tgcccccggt gttgaagttt tttttgatgc ttcgacagcg gtggactccg
    30481 cctcccggac catcgaatcc gccatgtgtt cggggaaaca cgttgtgatg atgaatgccg
    30541 aagcggacgc cgttttcggc ccctggttct ggcatctggc acagaaatac ggcgttgcct
    30601 atacgagcag cgacggtgat caacctgccg tgattgcgcg tttagtggaa gaattgcgat
    30661 tttacggtct cgactttgtg atggccggca acatcaaagg attccttgat cgttacaccg
    30721 atcccgtaaa aatcattcct gaggccgata aacgatttct tgatcataaa atgtgttctt
    30781 catttacgga tggaacaaag ctctgtgtag aaatgtcaat cgttgccaat gccctcggat
    30841 gctctgttgt tcaaccgggg atgctcggac cgaaaatgaa tgaaacaata gaactttttg
    30901 atcatttccc tctttccgat ctgtggacgc ccggtcaggc gccgctggtt gattatgttt
    30961 taggagcccg accccaagga ggcgtgtttg ccgttggcta tacggacgat gcctaccagc
    31021 gtcgcatgct gaattggttt ccgtctgaga caggacccgg gcctttttat gtgtttacac
    31081 gcccgtatca tctgattcat ttcgaaacca tgagaaccgt tctggaagtg gcccgtggcc
    31141 atcgctcgct aatggcgccg agattcggta tgagaacgga ggtcatctgt tatgccaaaa
    31201 aggcgctgga gccgggccat ctgcttgacg gtccgggagg attcgcttct tacggattga
    31261 tcgaaaacag cgacagcggt ctgcccgccg gattccccat tattttatcc gacggggtaa
    31321 aactccggag agctgtttcc cgggatcagc gggtgacgct ggacgatatc gacatctcca
    31381 cggtttcgcc cgacgcgctt gaatcctggc gcagggcctg caaaatcggc acacagaatg
    31441 tttagatttt aaagtatgat tctgaaaaaa accggcaaaa tgcctgaggc ggcaaaagga
    31501 tttttaatga tctgtgtttt taatgattcg aaaaaactca agcattatct tgttcgtagt
    31561 ctggaaaaac aaatgattcc gaagcagcgg attatttttt gtaatcgttc aacaaagaga
    31621 aaagttcttt ccatccgttt ctgaaaacaa aataggcgta attgcgcagg agattgtacg
    31681 tcgccttcct ttgcacggat ttcatgctgg ggccgtaaaa atctcttttt cccacatcgc
    31741 catgaaaaac aagcgtgccc tgatcgacgg ccactttgaa ccctttcttc cgcacccgga
    31801 tgagaaattc gctttcggtg tgatatccca ggccgtaaat ttcatttctt ccgccggctt
    31861 cctcgaagac agaccggggc gtcatcaaaa aacagccgac cagactgttg accgttgtga
    31921 tgtccccgtc atgcattttg acgagacgtt ctgctgccgc atcgattttc ttgaattcat
    31981 cggttgtaaa atcattcaag atgatccccg tatcaatctc ctggatcctg tgtccaaaag
    32041 cgaagctgag tttggggcct acggcgccat attgactgtt gctgcggagc agattcaaca
    32101 aacgggaaat ggcgtctttc ggcgcataga tgtcattgtt tgttaataac agatagggcg
    32161 ccttgctttg atcaacgcca cggttctgtg attttgcaat accctgattg gtctcgtttc
    32221 ttatcagcat tatgttcagg cccttttctt ttgcggtttt ttcgaccatg gcttttaatt
    32281 cattccgttt cggcgatgcg tcatcaacga taatgatctc aagattattt ttcatagaac
    32341 tgccggccag gctttccaga aacatctttt ccgttagatc aatcaaatat tgactgggac
    32401 aatagacggg cgtgatgatg ctgactttgt gatcgttttt atccacaggg cttactcttt
    32461 cacgatgctt tcggttggaa cggataaaac ataaagaacg ctttgccgta tgtcaagacg
    32521 atattatctc cgcaggaata tttttacgga caaagacgaa tgcgggaaga atgcaccgca
    32581 ttgacattaa aaggtgaaag aaagggccaa cgctccgaaa tgctgagcat ctttttgctt
    32641 ttcaaactct ttggtccggt gaacgtaaga gtaggtgatt ttgatccggt gaatgatgat
    32701 gcccaggcct ccgatgacct cggcgacgaa aggttctttt tcgacgctgt gactgtcgcg
    32761 gaacgtgttc ccgtcaagca ggatattgcg ggcgacggct tttcc
//
LOCUS       DAONQZ010000022        32253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1517_length_32253_cov_15.839431, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000022 DAONQZ010000000
VERSION     DAONQZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32253
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_32253_cov_15.839431"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLR13_05545"
     CDS             <1..321
                     /locus_tag="PLR13_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQC18833.1"
                     /translation="LGLDWKMITALLTGLVAKENVVATLGVLYSVGRDGLSNMLPAIM
                     SPESAAAFLVVMMLFIPCAATIAVLKKEMNNNKWFYTAIVMMLLVSYIGGIAAYNFVK
                     WLGV"
     gene            complement(326..1498)
                     /locus_tag="PLR13_05550"
     CDS             complement(326..1498)
                     /locus_tag="PLR13_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HQC18834.1"
                     /translation="MKKNILIRNVCRGFIFLFVVIPLLSCSTFTAARYDADVSPGHCL
                     PVFPDRDGWYGGDGAYSIKLDKDRTLWVFGDTFVGCPGCRRDRIDMDIVLGTTVAISS
                     CPENRGFEITYYLQHSGGKYVSSFGKNEWLWPQDPFMVNRVLYIPLISIVPGDKEDEV
                     FNFKVAGHKFVRVKDFSATDPRNWNYDYVDLTHALPPEIAAFAATSVVHDRYVFFYPF
                     YVHVKNDLNVFGNILARIPVDRIDRPAGAIEYYTKEGTWENRLSPEKVKIVLDASVSE
                     LSVRYHEDDQKWIAVYLSTQNKGDRLLYQTSDKLEGPWSEAKPLGAPVPEVDPRSALY
                     DKGNFCYAGKEHIGFSRGKYLVVTYVCNSSEDMEKQDSFIRRNMFLYRPVVREIGY"
     gene            complement(1503..2060)
                     /locus_tag="PLR13_05555"
     CDS             complement(1503..2060)
                     /locus_tag="PLR13_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HQC18835.1"
                     /translation="MGFITIFLIATGLGLDAFSVAIGIGAAHQKRSWIPVLRLAIAFG
                     FFQFIMPIIGWMAGLTVVEVIASFDHWIAFGLLALIGGKMILEGCGKGIDETQSDRTT
                     GWPLLMLSIATSIDALAVGFSFSVLKHPILFPAVVIGVVCFVMTATGMIFGKALAKLF
                     GRKVEIFGGIVLIVIGVKILFDHLM"
     gene            complement(2070..3920)
                     /gene="uvrC"
                     /locus_tag="PLR13_05560"
     CDS             complement(2070..3920)
                     /gene="uvrC"
                     /locus_tag="PLR13_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQC18836.1"
                     /translation="MMKENNVLKDKIALFPRSPGVYLMKDPAGKIIYIGKANHLKSRV
                     ASYFTGKDSRPMTPFLMARVYDIEFINTATAKEALILENNLIKRHRPRYNVTLRDDKT
                     YYYLSLDPTDKFPRLQLVRKRLHNAALYFGPYPSGLAAKETLRFVQQIFPLRSCRNRD
                     FKLRPRPCLEYQIGRCLAPCSGRINEEAYRKLADNAVSFLRGRSRELITELKKQMNDA
                     SEELHYEEAARLRDRIAALEHALEKQNVERGEEKDQDVFGFFSRHHHYQVCVLFVREG
                     KLLGSKSFAPIRLKIDASEIVSSCLTQYYDGDVTLPDEIIMPCHLPDEGVIAEWLSEK
                     KNKKMILTVPSIGAKNALLQLANDNARNLWESTYKKREEKEFALQILQEKLSLAGLPR
                     RIECYDISNIGGTYAVGSMAVLRDGDPDKNSYRRYRVTAPAEPDDYKMMQEVLSRRFS
                     SGEHLPDLVVVDGGKGQLNVALSVLKDLKIRTDVIGLAKEKCRRCTVRAGQKTTRTWE
                     DRVYLPGRKDALYLSAWPQALRILQLVRDEAHRFALRYHHQIKHKGDLHSVLDDIADI
                     GAKRRKILLSHFGSAGKIKDATKEELQNVPGIGKVLAEKIFNALKIIQNE"
     gene            complement(3981..4376)
                     /locus_tag="PLR13_05565"
     CDS             complement(3981..4376)
                     /locus_tag="PLR13_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18837.1"
                     /translation="MKYLIITGAVLILLAITTGWFIIAGKYLSLTVMKKIIRDEEKLI
                     KAHVDYVIMGILLLVLFATGINLPLVVAVLACAGALANPSLFLFLAVKPNVNKKAGSF
                     FGIVSTLVFLITTAGIGGAALLLIQELIQ"
     gene            complement(4417..5457)
                     /locus_tag="PLR13_05570"
     CDS             complement(4417..5457)
                     /locus_tag="PLR13_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18838.1"
                     /translation="MSRRLKGFLFILCFLLISFLVAELFFRYLYPKIAPFDDRIDVRY
                     ANPYTMFSRFAYGSQESAKTRGEFRIFMLGGSTVEYGNPPLPVYVEQELKRRGFENVR
                     VFNYGVISQNSGQELAHLVFHVLDSEPDMVVFYDGGNDIMDPLLYDPRPGYPFNFLAY
                     ENNVLFQATGDYPWPALLAYGSEMLRHVFRNYFFEKFGNFSNLRLSAQHGEYAWSEKI
                     AGIYAGNLAKAGQICRAYHVEYVAFLQPIIFYKRFAVSDTEKNILKGWPPEEAGIVRP
                     YTNDVRKRILRNIREEQKRFPFEFHDLSDIFCNSRDAVFTDYIHVNDTSNKKIALAIC
                     DRLMDNIKNSHP"
     gene            complement(5530..7344)
                     /locus_tag="PLR13_05575"
     CDS             complement(5530..7344)
                     /locus_tag="PLR13_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC18839.1"
                     /translation="MSVNYFIVYNPLTYQLMDLMPANSITPFDVITYLSGQNSHEVNA
                     ENYLRFIDFQPEKKRATMVDALAIEMYAGIYIQHQLAKLAQDHLVVMVDGKRRNFSEV
                     IRQKKAPPQAVFISTISSSFPAAAAAAIVLNHAKIPVIIGGIHVSTMPEDADTFIRKY
                     APYPELISIVRGAGDSTVLKTILDDLKTHSLKRDYSGETLIENGVWGNFDNLEPLEPL
                     KINLLNRLPVIKYTPLKDFRINPVAPFTGCIFSCKFCSISTLPQRNKIVQFRDTDDFV
                     EELRSYQKEIVDFKNRYYFFTPDNLFSNKKNLISFLKKIIASDLRINYAVQISIEVAD
                     DEELLKLIRASGGTHFFIGLESLDIRNLKHINKHIVKDIEKSGMSVKKYYASKIRKIH
                     AHGISVHGSFIVGLPYDYFNSPTDNTGVDITKFCIRNRIGAQPSTIVDLPGSVFFHES
                     QENNTFIYGKKGTMDYFLSLSVADLAETNKIPPDSLHQSPLIIASMVYEISKIVGAPV
                     RATFNSSIIFFRSLLHPTNKGQGLHFWQRFIDAVCSAVSQTAVALYKEQGESLVRSKH
                     GVRGIMERLYDTEKNPEIRKMFKNYIPQFTEYGTSGKT"
     gene            complement(7595..8227)
                     /locus_tag="PLR13_05580"
     CDS             complement(7595..8227)
                     /locus_tag="PLR13_05580"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HQC18840.1"
                     /translation="MPVRTYHLILLATVLIVWIWSAIKPQDTYLTWFLETLPFMMALP
                     IILLTYKKFRLTDLTYTLIALHAMILMLGAHYSYAKVPLGLWMEEWFGWTRNNYDKIG
                     HLMQGFGPAIYAREIIARTSPVQKGKWLAFFSLAVPLAFSALYEILEWAASLSNPNDT
                     EAFLGTQGYLWDTQTDMFLCLIGSMASLLLLATLHDKHLLKTQAPGINKK"
     gene            8406..10388
                     /locus_tag="PLR13_05585"
     CDS             8406..10388
                     /locus_tag="PLR13_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQC18841.1"
                     /translation="MKKIFLQFFLLLVLFGLLPVAGIFNPVAAEESARNASGNQTEID
                     LMLLFTNDLHSYFLPHRVLTEDGRQTVQGGYARLACMINEQRRLHPAKTLLVDAGDFS
                     MGTLFHTAFMTEAFELRLMGKMGYAATTLGNHDFDFHPEGLAKMLTAARSKSQILPQI
                     VASNLIVNGDHPGDKTLKQALNDYPVRDYMVLERNGLRIGLFGIMGKDAADDAPFAKP
                     VMFADPVEASKKTVAILKNREKADLVICLSHSGTSADAKKSEDEILARAVPQIDVIVS
                     GHTHTVLKTPIIIGRTIIVSGGRYGRYLGSLGIRFDRFKGASLASYDLRHVSSEIPED
                     KAVQEAIAGYEKIVNRDFLAHYDLTFEDVIAESDFSMESLESMHRHPRETGLGNLIVD
                     AYREAVKQAEGPDYRHVHFALQPLGVIRDSLLKGKITVSDVFRVLSLGIGPDRIPGYP
                     LVSFYISGKELKDILEVHTTIAPMHKKDAYLQVSGVKFTYNPRRIWFDRVTSVWVADE
                     KGVYQPLDQTKLYRACANLYTAALIDYVSSVSFGLIHVVPKDSSGKRLTDLNSSIVSM
                     KKTGEIKEWLALALYLRSFQEPQHLQTAKIPLKYSKPEGRYLAQPSCNPVRIFSKGNI
                     LTKVFLVSVILLLMICGLIVYVFVKKMKKISKKNPR"
     gene            10639..11385
                     /locus_tag="PLR13_05590"
     CDS             10639..11385
                     /locus_tag="PLR13_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQC18842.1"
                     /translation="MQIELTPVEKMKAPEQIAERLLKYILGGGVSPGEKLPPERTLAT
                     QLNVTRATLREALKKLEQLKLIVIHQGKGIIVEDFHKASIDLIFSLLVINGQIDLKIL
                     ENILEARELFGTDVARCAARRADKKDVEQMKILMNELIKATDPGKLQQLDFEFFRQLA
                     LASKNMVYILLMNTIKTIHDNHLDLFLPLSTNLDTTLQQEILQAVIDGDEEKAAEKAN
                     TFLKAGMELLKFLKSLSDTKESQNADRETN"
     gene            11363..14668
                     /locus_tag="PLR13_05595"
     CDS             11363..14668
                     /locus_tag="PLR13_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQC18843.1"
                     /translation="MQTARQIDKDPIEQTQKLNVLKIQRTCVHDGPGIRTTIFFQGCG
                     LRCVWCQNPEALSLKPMPSPAVHYAIADVLEVVLRDKDYYLKTGGGVTLSGGEPLLQD
                     PESLTHLLKKLKDEKIHIAVETSLHVPWENISKTAPYIDLFLVDLKVVGDEDLHRKCT
                     KQDSRLIHDNIRKLLASKANIKFRMVMVPEYNDHEQHIKATADFLKSVNHKSIELLKY
                     HNMYEDKAKRLGLVRESLNISPEQSLTAVKKAVELLKPLDIYAECEDLGNFRSKAVFT
                     DRVHAIQKDIRKSGYHLCFETSKLKTKFYRKNGFKKPTHIHRAERLDYVLKNKQVRIY
                     PHELLVGNFTSKRVGGQVWEEHYGILFSSILHQIHRQTPVAFQCSWKDRLYFYCRILP
                     FWQKHCLLTKVNKTLSDLVLSISRLCEMNVGFNNNMAAIAHFIVNFERILTLGTTGII
                     KELEEAQKEKPQNDQTFYQGAIIAVKALENFALRYADALSDMSKTEADPVRRKELEDL
                     VQICRHVPKHPARTYHEALQSMMFLQVALCIESYENAISFGRLDQILYPYYKKDKEAG
                     IITYEKARELLALFILKMDEAILVNDGDTYFAIGRLFETQSTDQTVTFGGVDKDGQDA
                     TNDLTYMLIDICELQFLAVDMSARIHPNSPERYLNRIAEVYINGSPQPKLNNDEVYIQ
                     SLSKHYDTSIENIRNYSIVGCVEPNASDDHFGNTDCANMNVVLPLLQALKGEEDDLWN
                     YGFSDQMEKLTTKFIEYNFRGGNRVSKWIADKYGKARASYKTKKKTAYNPPANMDELL
                     ERYQNRLNHLASSILADHQKIEKEICKNFTTPLASALSRGCVEKGLDVNQGGCEFNTS
                     GIQAVGITDAADSLHALNEVVFKKKMYSLMDVIHAIDNDFEGDDGQQIRQALLAVPKL
                     GHDDSPEAVKWVNKTLEIYCNALRQVPNSPRNGFYTAGYYALNVSDVYGKKTPSLPSG
                     RLKGVPLANSISPHYGMQMSDLLSSLNSVSGVDFVKYAPNGTTVTFTMDSALFQGRDG
                     ITNLAGIFSTYFKKGGMQFQPNVINREMLLDAYNHPEKYPYLLVRIAGYCAYFNHLSD
                     DLKKVIINRTCYS"
     gene            complement(14747..15394)
                     /locus_tag="PLR13_05600"
     CDS             complement(14747..15394)
                     /locus_tag="PLR13_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC18844.1"
                     /translation="MLKKYRYVLTDFLQDFLYYFIRGYSWTFRLQVVNEKPWMDHLKQ
                     GGRILICCWHQQFFSAIRHFKTYAPYHPALMISQSKDGNIIARIAEKTGWHTVRGSSS
                     KDGSRALRGMIDYLIQSRFAGHVVDGPRGPAGIVKAGVVKLAQASGALVVPFYISADR
                     AWYFNSWDHFMLPKPFAHVTLRFGDMLDLASGESEEDFERQRLRLEGIMKPNLHL"
     gene            complement(15538..16005)
                     /locus_tag="PLR13_05605"
     CDS             complement(15538..16005)
                     /locus_tag="PLR13_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18845.1"
                     /translation="MKTIKIYCIVILAALLLSAYGSLAADWGFRGLLPRGTGYYDKNS
                     IRKISDHIYQVWTVTIYNERGRADAYAMLLKQEKAPGNPAVLNQESILLELDCLQGKY
                     RIVSINIYDEKDTLLFSVPETNDGWHDIIPNSINEKLNNAVCKAVDNHRNGNP"
     gene            complement(16091..16972)
                     /locus_tag="PLR13_05610"
     CDS             complement(16091..16972)
                     /locus_tag="PLR13_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18846.1"
                     /translation="MKEETEINIPKEFTNKLFWQSLNPMSITRATDGMYVDVNESFTE
                     FFGRRREEVIGNTAADIAYATSLERLKILEEINEKGFARNITITVRDKKGDLRCALVN
                     TLPVEIKNQVYWLTVGTDISDLNPAAKISHEDALIKSLNSIKGAGVVLIRNHQKKQPS
                     LCYMNEEAKMVLKTHSLDNLLRTLAENDSTYLSIGAKYYHVKNINNHRRSPLKLIVME
                     RMPDTICIREKLTILDLTPRQKEIAFLAATGYSNREIAGRFCITEYTVKDHLKKIFHT
                     LGVKKRSEISPKLFHWR"
     gene            17381..17773
                     /locus_tag="PLR13_05615"
     CDS             17381..17773
                     /locus_tag="PLR13_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18847.1"
                     /translation="MKKRIMVLLSIIVFVLGLVCIQGCQQNENPKFQNEYQAVLLTTG
                     QVFFGKVEFVGTDYVLLKDVFYIRSVANPDTKQVSNTLIRKGNEIHGADQTYINARHI
                     IMIEPVSPQSPLAGRIKELQSQKPQGQP"
     gene            complement(17887..19071)
                     /gene="metK"
                     /locus_tag="PLR13_05620"
     CDS             complement(17887..19071)
                     /gene="metK"
                     /locus_tag="PLR13_05620"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQC18848.1"
                     /translation="MSKLHTRHIYTSESVSEGHPDKVCDQISDAILDACLEQDKHAHV
                     GCETAVGPNLVVNLGEITCKNWNKINPQAISRQVVQDIGYDDPCENFSWDTFNYLSAI
                     HGQSPDIKQGVDRDRPENQGAGDQGMMFGYASYATPQMMPAPIYYAHLLLEELTILRK
                     ANVIDYLRPDSKSQVSVLHEDGKAVKITTVVISHQTLDVPLKRIRKDLIDVAHQVLDK
                     TGMLTNKTEFFINPTGRFVIGGPAGDSGVTGRKIIVDSYGGVGSHGGGAFSGKDPSKV
                     DRSAAYYARYAAKNIVAARLADKCEIQIAYAIGVAKPLSINVCTYGTSVIPENKIEKI
                     LTEGKIFDFRPAMMIKDLGLTTPKGWSYRKACNYGHFGRKGFPWEKTDRVDALRQAAG
                     LK"
     gene            complement(19545..20105)
                     /locus_tag="PLR13_05625"
     CDS             complement(19545..20105)
                     /locus_tag="PLR13_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18849.1"
                     /translation="MKKLMSGIIFFAGVLFAPFSVSAGVSVHIDIPLPPPIIFPAPPH
                     LVVIPETYIYAVPDIQDDLFFYSGWWWRPWNGRWYRSRYYDRGWTYDHQPPFFHRTIP
                     PGWKDNYRNKTWKGHRWEHRKISHHELQRNWSGWKKEKRWEKHSWGVKNWKDRRSGTQ
                     YRREVKKSKTVIKYRDDRRPVREDRR"
     gene            complement(20260..21201)
                     /locus_tag="PLR13_05630"
     CDS             complement(20260..21201)
                     /locus_tag="PLR13_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC18850.1"
                     /translation="MRLLKAIVVLIILCVATGIGFVYLAPEQATSLALDVERKRSGLE
                     KKEINLSDHIRYVYLEGGKGEPLMLLHGFGANKDNFTRVARFLTPHYRVIIPDHIGFG
                     DSSRPQNEDYGSLAQAVRIRTLAQALGIKDVHVGGSSMGGHISMMYAFLYPDEVKSLW
                     LIDPGGIWSAPPGELHEIIAKTGKNPLMAKNEDEFAEIFSFVMTDHPFIPRPILNVMA
                     QERIRNYELEQRIFKQLSSDASERYVKEIKTPTLIVWGEKDRVISPQTAPILNKLLQN
                     SEIIIMKGLGHLPMIEQPKIAADDYLKFRKRLAKQGS"
     gene            complement(21280..22611)
                     /locus_tag="PLR13_05635"
     CDS             complement(21280..22611)
                     /locus_tag="PLR13_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQC18851.1"
                     /translation="MSIWNKKNTIVVTCPKGVSPFLKEEVQALNYPVRAEWDTAIQTE
                     GTLQDTMALNLHLRTAQRVLYQLDSFSADTPEWLYKKIHALPWENLLHDSGPAAYLCV
                     TSIAENPLIKDSQFVNVKVKDAIVDRLRDISGNRPDSGPEKNRAVVHVYWKDNRATVY
                     LDTSGERLALRGYRKVPLVAPMQETLAAAVIMAAGWKGESNFINPMCGSGTLAIEAAS
                     IALHRAPGLLRNHFGFMFIKGFPEDHWQTLRSKARTNALKKLPARIIATDINREAISA
                     ARQNAQTAGVDHFIEFNICPFEKTSVPAGDGIIIFNPPYGERMSSDPRKPVSADFRHS
                     RETPGPGRKIILRKSGDKSGDGNRHRGREMKKLEALYQGIGDFFKKTCTGCHGYIFTG
                     NLELIKKVGLRTKRRVILYNGDIECRLLEYALYTGSLKNTAKEKSLSAENG"
     gene            22790..24034
                     /locus_tag="PLR13_05640"
     CDS             22790..24034
                     /locus_tag="PLR13_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQC18852.1"
                     /translation="MKNDRKIFWGWYIVGGAFLVMAMNYGARYCFGVFLKPMANEFAL
                     SRSVISLAAAINMMVYSFGSVFVGRMLDRVAPRWITTAGAGVAACGYLLTGFVDTPIW
                     LYISYGVMVGLGSAGMGVVACSSSVSKWFISKRGVAVGIASMGISLGTVMLTPLAGYI
                     ATVFSWRFGLMALSFIMLIVGVVVAQTLMRRTHPEAYGLLPDGNTISGMPQAAEATAV
                     CPVSTMILFRDSRFWTLAVCQGLAVMISMAVFVHQVAYATDNGISKMAAASSLAAVSL
                     TGFMGQFFFGWITDRINDPKYASFLGISFMLAGTVILLNVESLLHLYVAALVYGFGYG
                     SLAPMLPLIIADRFGRQCMGSIYGLLTFFIGTGGAIGPVLGGFIYDHFGSYHNLWLMN
                     IVVLSLIAMAFLTLKKGRPYAA"
     gene            complement(24052..25215)
                     /locus_tag="PLR13_05645"
     CDS             complement(24052..25215)
                     /locus_tag="PLR13_05645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC18853.1"
                     /translation="MQNFVFENPTKIIFGKGQIPRIGKETARFGRRALLVFGMGTILK
                     NGVYDQVTNALEKAGVEIVDFPGVKSNPVLSHVQKGIALARGENVDVILAVGGGSVID
                     AAKAIAAGVVAHHDVWDFFTFSKPVTGALPVLTVATVSASASEMNAGAVVTREDGCQK
                     YCIVSPFIQPKVSVLDPTVLFSLSPAYSAYSAVDIITHMLEGYFNNTSSASPLQDRLV
                     ESLIKTVMESTEIILKEPDNYDARANMMWSAGLAFNGLTTAGMGQVRMPAHMIEHSLS
                     AIYDIAHGAGLSIILPAWMSYALDKKTDRLEKLAREIFHVKGRGAISAAKKGIACLKH
                     WFESIGSPVSLQAVDIPESDIDAIAGNAFALSQVWCYEGYTKDVIRKILLLAR"
     gene            complement(25283..26512)
                     /locus_tag="PLR13_05650"
     CDS             complement(25283..26512)
                     /locus_tag="PLR13_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18854.1"
                     /translation="MNGSNFQIEQQCPQCGAPIILDETDRILSCHFCRTRVYLATDDH
                     FLFYIPPAAGISEKIYFIPYWRLKGLSYAITTHNIAYKYFDANFISFDLPFFPHSLGL
                     RPQAMKLRFVGNADHEGKFIAASQKTRGKLLKNFKTQAGAVQEIFIGETASIIYTPVY
                     FANGRLYDAVLKKPLDVQKKEPEMEDKLVSSPDEKWHVDFLPLLCPECGADLPGAKDA
                     LIVFCENCHRAWNPSKKHFVPIPYATWREKGDEITYLPFWQLKVKVDGIALGNYADFI
                     KVANLPKAPLDIWDKTPFYFFAPAFKLNPALFLRWCRQLTVTPAPDDLTPDCPVKNIH
                     QVTLPVSEAFESCLVTLGSLMADKRHLADMLSSLKLTLEHYSLVLHPFAVRNRELVHT
                     KLDFSMDISALTMGAFL"
     gene            complement(26499..27617)
                     /locus_tag="PLR13_05655"
     CDS             complement(26499..27617)
                     /locus_tag="PLR13_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQC18855.1"
                     /translation="MGTNNIIFLEVIEWFDESGKEIVHRIPEKGSGEIKFGAQLTVRE
                     SQSAVFFYKGKAYDAFGPGRHTLKTANIPLLTKVIAIPWGMTSPLRAEVYFINMKVFP
                     NLKWGTRDPVAFRDRELGLIRLRAFGVFNIRVIQPLLLINSLVGTQGIFSTEEIEEYL
                     NRVIVSRFNDYMGEKVDTIFNLPGKYNEFSEGLKVMLQKDLSKFGLALTDLYINSITP
                     PPEVQKAIDDKSRLGVFDDLNKLLKMKAAMAVEAAAQNQSEAGAGLGMGMGFMMPGML
                     LEAFKGAGTTVAADVFTCPDCRQVVPKEAHFCPACGHQILIVNKCAHCGRNLPVNANF
                     CVSCGKPVTVNPPGKQCAKCGFENLPDSMYCNKCGERL"
     gene            27764..29191
                     /locus_tag="PLR13_05660"
     CDS             27764..29191
                     /locus_tag="PLR13_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC18856.1"
                     /translation="MKKTSAEQNIYDGLALIADPIHQYILFTVPKDKSAKSAGEITEQ
                     HIIDSPWMQRLRRIYQLQSARWVYPAAEHSRFQHSLGTMHMAGEFAKSIYPSLSSVCK
                     DVPSCHYVEELLRLAGLLHDVGHGPFGHFFDDNFLSNWSLTHEIVGQNIIVDKLGDDL
                     ARVRRSPSGGFSRTERMDAQKVAYLIRMPEDGEEGRPPRWLQMLRQLFSGVYTADNLD
                     YVQRDAYMTGFSLDMVDIPRLRYYTFFTREGLTLHQSGISALRRFLNARLNLYTNVYF
                     HRTTRGLDLHLQEFFHDTMQMILPANPLNNLDDYLQCDEWTLFNHVQNWLRSKDEQKK
                     KLAAEWKKLHDREVKWKMSFVAEISLDQIQPGVGFSRAADYEQQIRERLPKPLKSLPF
                     KVDLATQDPRPLNPITEREKRINIFNPVTHKTSPEPLLEIYRFIPARVMQFRVFALNH
                     DHDGDLAAAAEKVLGSSAKSTLTNI"
     gene            29193..31253
                     /locus_tag="PLR13_05665"
     CDS             29193..31253
                     /locus_tag="PLR13_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HQC18857.1"
                     /translation="MSKNDETTDLTLFVRTTGEEIVLWDRQRIVDALVREADIDDDVA
                     GQISKEVEKQIIVSGIGLLTTALIRELVNVRLIERGLEKERRLHGRLGFPLYDVRQLI
                     LHPNKQDANIPHSPEGTNLIFAEGIKRELSLFDVFSADVGEAHATGDIHIHALGYIDR
                     PYSCCQSLEYLKINGLNLPHAINSANPAKYAEVLLAHMVRFSAVLQGHFAGTVGWDAV
                     NISFAPYLASMTDKEVKQFAQMLIYEFSQLAATRGGQALYTDIHIYYDVPPHWADLPA
                     VGAEGKPTGKTYGDYTRDAQRFAMAILEIFAEGDARGKPFILPRPLLHITDHVWKNDK
                     AREFLLRACDVAALMGNPCFVFDRDEKAVDLIFNDEERRKPWLQRRAAIQTVTLNLPR
                     LGYKAGGDEKEVFSLLEAFMEKAVKAHVQKKDFLEKLLSYAEEGPLAVLAMNHDGFPF
                     LRMHLASYVIGIVGLNELVQIVTGNEMHQKQEALEFGLRVVDFIRQEALRLSRQCKMK
                     FVLGQSLAETTAYRFARLDLRYFSPTAGRYVKGDIAEGAIYYTNSTHLNVAADVGPLQ
                     KVMREGLFHHYLEDEVITHLQLGDLNPGRNVLARFIRDVFYHSANRQIDFNPEFTFCL
                     SCKETTRGLQDKCAACGSADVEGIARLTKYFSKTSGWNKGKLAELKDRTMNDNFNNVD
                     QPKC"
     gene            complement(31250..32173)
                     /locus_tag="PLR13_05670"
     CDS             complement(31250..32173)
                     /locus_tag="PLR13_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQC18858.1"
                     /translation="MKKILLKIINPVVLFNAWLYHKKNSTFSNSTSDLELHLYSKIFK
                     NDMLHYGYFENTEIEVDTISIAEIEKAQVRYAQNIIDQIINPQDLILDIGCGMGGLSN
                     LISQKGFNVEALTPNKNQIRHIQSKYPTITCYQKRFQEFETDKKYGTIINSESLQYIP
                     LEKAFEKAESIVKPDGTWIIADYFKFNNSGVNKSSHLLDDFVRKSKEHGWEITYQQDM
                     TPHVLPTLKFVYNYAERFLLPLVHFTSGKIKRKKAWLYYLIRDLKQSVDHKIWKELAA
                     VDPIKFRNEKKYMLFVLRKNCRDAADNPASS"
BASE COUNT         8120 a         7634 c         7907 g         8592 t
ORIGIN      
        1 ctgggactgg actggaaaat gattaccgct cttttgaccg gtctggtggc gaaagaaaat
       61 gtcgtggcga ctctgggcgt gctttattcc gtgggcagag acggtctgtc caatatgttg
      121 ccggccatta tgagtccgga atcggctgcg gcgtttcttg tggtcatgat gcttttcatc
      181 ccctgtgccg ccacaattgc cgttttgaaa aaagaaatga acaataataa atggttttat
      241 actgctattg tcatgatgct gctggtgtcg tatatcggtg gaatcgcggc ttataatttc
      301 gtcaaatggt tgggtgttta atcaatcaat aaccgatttc tctaacgacc gggcgataga
      361 gaaacatatt gcggcggata aagctgtcct gcttctccat atcttctgat gaattacaga
      421 cgtaagtcac cacaagatat ttccccctgg aaaatccgat gtgttctttt cccgcgtaac
      481 aaaaatttcc tttatcgtac agagcgcttc ggggatccac ttccgggacg ggagcgccca
      541 gaggtttcgc ttcactccac ggaccttcca gtttgtcgga ggtctgatac agcaaccggt
      601 cccctttatt ttgggtgctt aaataaacgg cgatccactt ttgatcatct tcatggtagc
      661 ggacgctcaa ttccgagaca ctggcgtcga gaacgatttt aactttttcc gggctcagcc
      721 ggttttccca ggttccttcc ttggtgtaat attctatcgc cccggcagga cgatcgattc
      781 tgtcgacagg aatgcgggcc agaatatttc cgaagacatt caaatcgttt ttgacgtgaa
      841 catagaaagg gtagaaaaaa acatagcgat catgcacaac ggatgtggcg gcaaaggcgg
      901 caatttccgg aggcagcgcg tgcgtcaaat cgacgtaatc ataattccaa ttgcggggat
      961 cggtcgcgga aaaatctttt acccggacga atttgtgacc ggcgactttg aaattgaata
     1021 cctcatcttc tttgtctccc ggaacaatcg atatcagcgg aatatataaa acgcggttaa
     1081 ccataaacgg atcctgcggc cagagccatt cattcttgcc gaacgatgac acatatttac
     1141 cgccgctgtg ttgcagataa taggtgatct caaatccgcg attttccggg caggaggaaa
     1201 tcgcaacggt ggtgcccagg acaatgtcca tatcaatgcg gtctcgtctg catcccgggc
     1261 agccgacaaa cgtatctccg aaaacccaga gtgtccgatc cttgtccagt ttaatggagt
     1321 aggcgccgtc gccgccgtac cagccatccc tgtcgggaaa aaccggcagg caatgacccg
     1381 gcgagacgtc cgcatcgtat cttgcggcag taaacgtgga gcaggaaaga agaggaataa
     1441 caacgaacag gaaaataaag ccccggcaga cattccggat caaaatattt tttttcataa
     1501 catcacatca aatggtcaaa tagaattttt acgccaatca caattaaaac aattccgccg
     1561 aagatttcca ctttccggcc gaataatttc gccagcgcct tgccaaaaat catgccggta
     1621 gccgtcatga cgaaacagac gacgccgatg acgacggcag ggaacaaaat cggatgtttc
     1681 aagacggaaa aactgaaacc caccgccaga gcgtcgatgc tggtcgcaat ggagagcatc
     1741 agcagcggcc agccggttgt ccgatccgat tgtgtttcgt caatgccttt tccgcaacct
     1801 tccaggatca ttttcccccc gatcagcgcc agaagtccga atgcaatcca gtgatcgaag
     1861 gaggcgataa cctccacaac ggtcagaccg gccatccagc cgatgatggg catgataaac
     1921 tgaaagaatc cgaaagcaat cgcgagccgc aacaccggga tccatgatct tttctgatgg
     1981 gcggcgccga taccgatcgc gacggaaaat gcgtccagtc ccagtcctgt cgcaatcaag
     2041 aatattgtta tgaagcccat gacggtccat cattcattct gaattatttt cagagcgtta
     2101 aagatttttt ctgccaaaac cttaccaatt cccgggacgt tttgcaattc ttccttcgtt
     2161 gcatctttta tttttccggc agagccgaaa tggctcagta aaattttgcg gcgttttgca
     2221 ccgatgtcgg cgatgtcatc aagtacggaa tgcaaatcgc ctttatgttt gatctgatga
     2281 tggtaacgta atgcgaaacg atgggcctca tcccgcacca gttgaagaat gcgcaacgcc
     2341 tgcggccagg cggataaata gagggcatct ttgcgtcccg gcaggtagac gcgatcctcc
     2401 cacgtccggg ttgtcttttg tcccgcgcgg acggtacagc gccggcattt ttcttttgcc
     2461 aggccgatga catccgtcct gatcttcaga tctttcagca ccgaaagcgc gacgttgagt
     2521 tgccctttcc cgccgtcgac aacaaccagg tcaggcaggt gttcaccgct ggaaaaccgt
     2581 cgggaaagca cttcctgcat cattttataa tcgtccggtt ccgccggcgc cgtgacccgg
     2641 taacgccggt aactgttttt gtcggggtca ccgtcccgaa gaaccgccat ggaaccgacg
     2701 gcgtatgtgc ccccgatatt ggaaatatca taacattcaa tacgtcgcgg caatccggcg
     2761 agtgataatt tttcctgcag tatttgcagg gcgaattctt tttcttcccg cttcttataa
     2821 gtggattccc agaggttgcg cgcattgtcg ttggccagct gaagcagggc gttttttgct
     2881 ccgatcgagg gcaccgtcag gatcattttt ttatttttct tttcactcag ccattcggcg
     2941 atgacgcctt catccggcaa atggcagggc atgatgattt cgtcgggcaa cgtgacgtca
     3001 ccgtcataat actgcgtcaa acatgaggag acaatttcgc tggcatcgat tttcaacctg
     3061 atcggagcga atgatttgct gcccagcagt tttccctctc ggacgaataa aacacagacc
     3121 tggtaatgat gatgtcggct gaaaaaaccg aagacgtcct gatccttttc ttctccccgt
     3181 tcaacgtttt gtttttccag cgcgtgttcc agcgcggcta tccggtcgcg cagacgggcg
     3241 gcttcttcgt aatgcaactc ttctgacgcg tcgttcatct gttttttcag ttcggtaatc
     3301 agctcccggc ttcgtccccg cagaaacgat acggcgttgt cggccagttt ccggtaggcc
     3361 tcctcgttga ttcgtccgct gcaaggggca agacaccggc cgatctggta ttccaggcag
     3421 ggcctcgggc gaagcttaaa atccctgttg cggcaggaac gcaaaggaaa tatctgctgg
     3481 acgaaacgca gggtttcttt ggccgccagt ccggaagggt aaggaccgaa ataaagcgca
     3541 gcgttatgta atcgtttgcg caccagttgc agacggggaa atttatccgt cggatccagc
     3601 gacaggtaat aataggtttt atcgtcgcgc agcgtaacgt tgtaacgcgg gcggtggcgc
     3661 ttgattaaat tgttctcaag aatcagagct tctttagccg ttgcggtgtt gatgaattcg
     3721 atgtcataaa cgcgcgccat tagaaagggc gtcatcggac ggctgtcttt ccccgtgaaa
     3781 taagacgcca cacggctttt taaatgattt gctttgccga tatagataat tttcccggcc
     3841 ggatccttca taaggtagac gccggggctg cgcgggaaaa gagcgatttt atctttcagg
     3901 acgttgtttt ctttcatcat ttcagaaggc gacgaccatt ttgcaccggt cgtcagccgt
     3961 gtctgtctta gagtttcggg ttattgtatc agttcctgaa tcagaagcaa agcggctccg
     4021 ccgattcccg cggttgtgat taaaaatacc aacgtgctga cgataccgaa aaagcttccg
     4081 gcttttttat tgacgttcgg tttcacggca agaaacagaa aaagtgacgg attggccagc
     4141 gcccccgcac atgccaatac agcgacaacg agaggcaggt taatgccggt ggcaaaaagc
     4201 acgagcagga ggatgcccat tatcacataa tcaacgtgag ccttgataag cttttcttcg
     4261 tcgcggatga ttttcttcat gacggtcaga gataggtatt tgcccgcgat aatgaaccag
     4321 ccggtcgtta tcgccagcag aattaatacg gccccggtta tgatgagata tttcatgata
     4381 ttacctccgg aagatattag tgtcaggtct tattattcat ggatggctgt tttttatgtt
     4441 gtccatcagc cggtcacaga tagccagcgc aattttttta ttggaggtat cattgacgtg
     4501 gatgtaatcg gtgaagacag catcccggga attgcagaat atgtcactga gatcatgaaa
     4561 ttcaaaggga aaccgttttt gctcttcccg gatgttgcgg agaattcttt tccggacgtc
     4621 gttcgtatat ggacggacaa tgcccgcttc ttccggcggc catcctttta aaatattttt
     4681 ttccgtatcg gaaacggcga aacgtttata gaagattatg ggttgcagaa aggcaacata
     4741 ttcaacatgg taggcgcggc agatttgtcc cgcttttgcc agattgccgg cataaattcc
     4801 ggctattttt tccgaccagg catattctcc gtgttgtgcc gaaagtctca ggttggaaaa
     4861 attgccgaat ttttcaaaaa aataattgcg gaacacgtgc cggagcattt cgctgccgta
     4921 tgcgagcagc gcgggccagg ggtaatctcc tgttgcctga aatagaacat tgttttcata
     4981 agcaaggaag ttaaagggat agccgggccg gggatcgtaa agcagagggt ccataatatc
     5041 gttgccgccg tcgtagaaca ccaccatgtc cggttccgag tccaacacgt gaaagacaag
     5101 gtgagccagt tcctggccgg aattctgcga tatgacgccg tagttgaaga cgcgcacgtt
     5161 ctcgaatccg cggcgcttca gttcctgttc gacatagacg ggcaacggcg gattgccgta
     5221 ttcgaccgtc gaaccgccga gcataaatat tctgaattct ccccgcgttt tggccgattc
     5281 ttggctgccg tatgcgaatc gactgaacat cgtgtaggga tttgcataac ggacatcgat
     5341 cctgtcgtca aaaggggcaa ttttaggata aagataccgg aaaaacaatt cggcgaccaa
     5401 aaaagaaatc agcagaaaac aaagaatgaa gagaaatcct ttaagccgtc tggacatgat
     5461 ggctcctgcc gcataaaatc aaccaggatg tatggtctgt tccggaaaac attactgccg
     5521 ctgccttttt tatgtttttc ccgatgttcc gtattcggtg aattgcggga tgtagttttt
     5581 aaacattttc cgtatttcag gatttttttc cgtgtcataa agccgttcca taatgcctct
     5641 tacaccgtgc ttcgaccgta caagagactc gccctgctcc ttatacaggg caacggcggt
     5701 ctgggataca gccgaacaga cggcgtctat gaacctttgc cagaaatgca atccctgccc
     5761 tttatttgtg gggtgcaaca gagaccggaa aaaaatgatg gacgagttga acgttgccct
     5821 caccggtgcg ccgactattt tcgatatttc atagaccatg gacgcaataa tcagcggact
     5881 ttggtgcagc gaatcgggcg gaattttgtt ggtttcggcc agatcggcaa cggaaaggga
     5941 gagaaaataa tccatcgtgc cttttttgcc gtaaatgaac gtattattct cctggctttc
     6001 atggaaaaag accgagccgg gcaagtcaac gatggtgctg ggctgcgctc cgattcggtt
     6061 tcggatacag aattttgtaa tgtcaacgcc ggtgttgtcg gttggagaat taaaatagtc
     6121 atagggcagg cccacaataa aagatccgtg aacggaaatg ccatgagcat gaatcttcct
     6181 gattttggat gcgtaatatt tttttaccga catgccgctt ttttcgatat ctttaacaat
     6241 atgcttattg atatgcttca aattgcgtat atcgagggat tccagaccga tgaagaaatg
     6301 ggtgcctcct gatgctctga tcagttttag taattcttcg tcgtcggcaa cttcaatgga
     6361 gatttgaacc gcgtagttaa tgcgcagatc gctggcaatg attttcttta agaaggatat
     6421 cagatttttt ttattggaga acagattgtc cggcgtgaag aaatagtacc ggtttttaaa
     6481 atcaacgatt tccttctgat aagaacgaag ctcctcaaca aagtcgtccg tgtcccgaaa
     6541 ctggacgatt ttatttctct gcggcagagt cgatatggag caaaacttgc aggagaatat
     6601 acatccggtg aatggtgcga caggattaat ccggaaatct ttcagggggg tgtatttgat
     6661 caccggcagt ctgttgagca gattgatttt cagcggttcc aggggctcaa ggttgtcaaa
     6721 atttccccag accccgtttt caatcagggt ttctcccgaa tagtcccttt tcagggaatg
     6781 cgttttcaaa tcatccagga tggtcttgag gaccgtcgaa tcgcccgcgc ccctgacaat
     6841 ggaaatcagt tcgggatagg gcgcatattt tctgatgaac gtgtccgcat cttcaggcat
     6901 ggtggaaaca tgtatcccgc cgatgatgac aggaattttt gcgtgattca gcacgatggc
     6961 cgcagcagcg gcggctggaa aactggagct gatggtggaa ataaaaacag cctggggagg
     7021 ggcctttttc tgtctgatga cttctgaaaa attccgtcgc ttaccgtcca ccatgactac
     7081 gagatgatcc tgggccagtt ttgccagctg atgctggata tagatgcctg catacatttc
     7141 gatggccagg gcgtcaacca tggttgcccg ctttttttcc ggctgaaaat cgatgaatct
     7201 taaataattt tcagcattca cttcgtgcga gttctgtccg gacagatagg taatcacatc
     7261 aaacggcgtg atggaattgg ccggcatcag atccatcaat tgataggtca gcggattata
     7321 aacgataaaa taatttacgg acattgttta attgagccct ttctcatatt ttcagtaaaa
     7381 acattccagc ccatatacag aaccatgggg tatatcgtga ggacgatgcc cattgaagcg
     7441 gacacgtatc cggatttgct aaatcgccag tctcaatgtc tttcttgagc ttttatgatt
     7501 tttattgtgc aagacatagc acagagtggt ttcctcaaac aagcttaatt ttaatcatcc
     7561 tttcggtttt tttatttgtc ttgcgggaaa atgtttattt tttgtttatc cccggtgcct
     7621 gcgttttcaa aagatgcttg tcatgcaacg tggctaacag gaggagcgac gccatcgaac
     7681 cgatcagaca taaaaacatg tcggtttggg tatcccagag atatccctgg gttcccagaa
     7741 aagcttccgt gtcgttgggg ttggagagcg atgcggccca ttccagaatc tcatataatg
     7801 cggaaaaggc cagggggacg gcaagggaga aaaaagccag ccattttccc ttttggaccg
     7861 gtgaggttct ggcgataatt tcacgcgcgt atatcgccgg accgaacccc tgcatgagat
     7921 gcccgatttt gtcgtaatta ttccgcgtcc agccgaacca ttcttccatc cagaggccca
     7981 gcggtacttt ggcgtacgaa taatgggcgc cgagcatcag gatcatcgcg tggagggcga
     8041 ttagcgtata ggttaaatcc gtcagtctga atttcttgta tgtcaagagt atgatcggca
     8101 aggccatcat gaaaggcagg gtttccagaa accatgtcag ataggtgtct tgcggtttta
     8161 tggccgacca gatccagacg atcaagacgg ttgccagcaa gatcagatga tatgtacgta
     8221 cgggcatatt ttcttacggt caatgtgcgt gaagaatttt actgcaatct acttcattca
     8281 acggaaaaaa taaagaacga tttatggtgc ttcggaccat gatgattgga gtccattatt
     8341 tcttgatttt gtgatacatc accatacaga acataaaaaa gttgccggag cgtaaaagac
     8401 acaggatgaa aaaaattttc cttcaatttt ttctgctgct cgttttattc ggtttgttac
     8461 cggttgccgg aattttcaat ccggttgctg cggaagaatc tgcacggaac gcctccggga
     8521 atcaaacgga aatcgatctg atgcttctgt ttaccaacga tttacattct tactttctgc
     8581 cgcatcgcgt tttaacggaa gacggacgac agacagtgca ggggggatat gccagacttg
     8641 cctgtatgat caacgagcag aggcgcctgc atcccgctaa aaccttgctg gtcgatgccg
     8701 gcgatttttc gatgggcact ctttttcata ccgcgttcat gaccgaagct ttcgaactga
     8761 gactgatggg gaaaatgggc tatgccgcaa cgacgcttgg aaatcatgat ttcgactttc
     8821 atcccgaggg cctggcaaag atgctgacag cggcgcgttc caaatcgcaa atactgccgc
     8881 agattgttgc ctccaatctt attgtgaacg gtgaccatcc aggcgataaa acgctgaagc
     8941 aggcactgaa cgattatccg gtccgggatt acatggttct tgaaagaaac ggtttacgca
     9001 tcggcctgtt cggcatcatg ggaaaagacg cggccgacga tgctcctttc gccaaaccgg
     9061 tgatgtttgc cgatccggtg gaagcaagca aaaagaccgt tgcgatcttg aagaaccggg
     9121 aaaaggctga tctggtcatt tgcctttccc acagcggaac gtcggccgat gcgaagaaat
     9181 cggaagatga aattctggcc cgggcggttc cgcaaataga cgttatcgtc agcggacata
     9241 cgcacacggt cttgaaaacg ccgatcatca tcggccggac cattattgtc tccggcggac
     9301 gctacggcag gtacctcgga tcgctgggca ttcgttttga ccggtttaag ggcgcttcat
     9361 tggcttcata cgatctaaga catgtttcat cagaaattcc tgaagacaag gcggttcagg
     9421 aggcaattgc cggctacgaa aagatagtca atcgcgactt tcttgcacac tatgacctga
     9481 cattcgaaga cgtaattgcc gaatcggatt tttccatgga atctcttgaa tccatgcatc
     9541 gtcatccgcg tgaaacagga ctgggtaatt taatcgttga tgcctatcgt gaagccgtca
     9601 aacaggcgga aggtcccgat taccgccatg tccactttgc cctgcaacct ttgggcgtga
     9661 ttcgcgattc cctgctcaag ggaaaaatta ccgtaagcga tgtgttccgg gtcctttctc
     9721 tgggaatcgg accggacagg attcccgggt atccgctggt ttctttttat atcagcggga
     9781 aggaactcaa ggacattctg gaagttcata caaccatagc gccgatgcat aagaaagacg
     9841 cttatttgca ggtgtccggc gtgaaattta cctataatcc gcggcgcatc tggttcgacc
     9901 gggtcacgtc ggtttgggtt gcggatgaaa aaggcgttta tcaaccgctt gatcagacaa
     9961 aactctaccg cgcctgtgcg aatttgtata ccgccgccct tattgattac gtcagctcgg
    10021 tcagtttcgg tttaatccat gtggtgccga aggacagctc agggaagcga ttgaccgatc
    10081 tcaactcgtc catcgtgtct atgaaaaaaa ccggcgaaat caaggaatgg ctggcccttg
    10141 cactttatct gcgttcattt caagaaccgc aacatttgca aacggcgaag attccgctta
    10201 aatacagcaa accggaggga cgatatctcg cgcagccgtc ctgcaatccg gtcagaattt
    10261 ttagcaaagg caatatcctt acaaaggttt ttctggtgtc tgtaatccta ctccttatga
    10321 tttgcggttt aattgtctat gtttttgtca aaaaaatgaa aaaaatcagc aagaagaacc
    10381 cccgataaat gtcattcccc tgattaaaat agatattgtc tgtatttaca ataatttgta
    10441 gaaatatttt attattttga cattcattac tgccaaaaaa aataaaaata cttgattttt
    10501 gaaaattatc cacgtacaat tggtccaatg gtactaccaa tgtttatatt ttttgacacg
    10561 ctaaagatcg tttttttgat ggtgatccct cctcaaagat agattttcca attgcggtgc
    10621 cagaaggtga agaagaccat gcagatagaa ttaacgcccg ttgagaaaat gaaggctccg
    10681 gagcagattg ccgagaggct cctgaaatat attctcggag gcggcgtcag tcccggtgaa
    10741 aaattgcctc cggaaaggac cctagcgaca caattaaatg tgacgagggc gacactcagg
    10801 gaggctttga aaaaactcga gcagttgaaa ctgattgtca ttcatcaggg caaggggatt
    10861 atcgttgagg attttcacaa ggcctcaatc gacctgattt tttcattgct ggtcatcaac
    10921 ggccaaattg atctcaagat tctggaaaat attctggaag caagagaact gttcgggaca
    10981 gacgttgcca gatgcgcagc ccggcgagcc gacaaaaagg atgtcgagca gatgaaaatt
    11041 ctgatgaatg aactgatcaa ggcaacagat ccgggaaaac tccagcagct ggatttcgaa
    11101 tttttccggc agctcgcgct ggccagcaaa aacatggtgt acattcttct gatgaatacc
    11161 atcaaaacaa tccatgataa tcatctggat ctgtttctgc ccctgtcgac gaatctggat
    11221 acaacgcttc agcaggaaat tctgcaggct gtgatagacg gcgatgaaga aaaggcggct
    11281 gaaaaagcca acacttttct caaagccggt atggaattac tgaaattttt aaagtcgctt
    11341 tcagacacaa aggagtccca aaatgcagac cgcgagacaa attgacaaag acccgatcga
    11401 acaaacccaa aaactcaacg ttctgaaaat acagcgtacc tgtgttcatg acggtcccgg
    11461 catccgcaca accattttct ttcaggggtg cggactgcga tgcgtatggt gtcagaatcc
    11521 cgaagcgctg tctttaaaac cgatgccctc tcccgccgtg cattatgcaa ttgctgacgt
    11581 cctggaggtt gtcctgcgcg acaaggatta ttacctcaag accggcggcg gcgtgacgct
    11641 cagcggcggt gaacctctgc tgcaggatcc ggaaagcctt acccatcttt tgaaaaaatt
    11701 gaaggacgaa aagattcaca ttgctgtgga aacctccctg catgtgcctt gggagaatat
    11761 cagcaagaca gcgccctata ttgacttgtt cctggtcgat ttgaaggttg tgggggatga
    11821 ggacctccac agaaaatgca caaagcagga cagccgtctc attcacgaca atattagaaa
    11881 actgcttgcg tcaaaagcga acattaaatt ccgcatggtc atggtcccgg aatataacga
    11941 tcacgagcag cacataaagg cgacggctga ttttctgaaa tccgtaaacc acaaatcgat
    12001 tgaactttta aaatatcaca atatgtacga agacaaggcc aagcgtctcg gtctggtccg
    12061 tgaatcgttg aatatttcac cggagcagag tttgaccgcc gttaaaaaag ccgtcgaatt
    12121 attgaaacca ttggatattt acgccgagtg tgaagatctg ggaaactttc gcagtaaagc
    12181 ggtttttacc gatcgtgttc atgcgataca aaaagatata cgaaagagcg gttatcatct
    12241 ctgttttgaa acatccaaac tgaaaacaaa attttacaga aaaaacggtt tcaaaaaacc
    12301 gacccacatc catcgcgcgg aacgtctcga ttatgtgctg aaaaataagc aggtcaggat
    12361 ctatccgcat gaactgctgg tcggtaattt tacatccaaa agagtcggcg gtcaggtgtg
    12421 ggaggaacat tacgggattc tcttttcatc cattctccat cagattcacc gccagacacc
    12481 ggtggccttc cagtgttcgt ggaaagacag actgtatttt tattgcagaa tattgccgtt
    12541 ctggcagaaa cactgcctgc tgaccaaagt caataaaacg ttgtccgatc tggtcttaag
    12601 catttcacgg ttatgtgaaa tgaatgtcgg attcaacaac aatatggcag cgatagcgca
    12661 tttcatcgtc aacttcgaac gtattctgac acttgggaca accggcatca tcaaagaact
    12721 tgaagaggca caaaaagaaa agcctcagaa tgatcagacc ttttatcagg gcgccataat
    12781 tgccgtcaaa gcgctggaaa attttgcgct aagatacgct gacgctcttt ccgacatgag
    12841 caaaacagaa gcagaccccg tccgccgtaa ggaactggaa gacttggttc agatttgccg
    12901 gcatgtaccg aaacatccgg cacgcacgta tcatgaagcc ctccagtcca tgatgtttct
    12961 gcaggtcgcg ctctgtattg aatcgtatga gaacgccata tcgttcggac gtctcgatca
    13021 gatattgtat ccttattata agaaagataa agaggccgga atcatcacat acgaaaaagc
    13081 cagggaactg ctggccttat ttatcctcaa aatggacgag gcgattctgg taaacgacgg
    13141 agatacgtat tttgccatcg gccggctttt cgaaacccag tccacagacc agaccgtgac
    13201 gttcggcggt gtggacaaag acggtcagga tgcgaccaat gatcttacct atatgttgat
    13261 tgatatttgc gaactccagt ttctcgcggt ggatatgtcg gcaagaattc atccgaacag
    13321 ccctgagcgc tatctgaaca gaatagcgga ggtctatatt aacggttccc cgcaaccgaa
    13381 actgaataat gatgaagttt atattcaatc gttatcaaaa cattatgaca cgtctatcga
    13441 gaatatcaga aactattcca ttgtcggatg cgtcgaaccc aatgcgtctg atgatcattt
    13501 cgggaacacc gactgcgcca atatgaatgt cgtcctgccg ttgcttcagg ccctcaaagg
    13561 cgaagaagat gatttatgga actacggctt ttccgatcaa atggaaaaac tgacgacgaa
    13621 attcattgaa tataatttcc gcggcggcaa cagggtttcc aaatggatag cggacaaata
    13681 cggcaaagcc cgggcctcct ataaaacgaa aaagaaaaca gcatataatc ctccggcgaa
    13741 tatggatgaa ttgttggaac gatatcagaa tcgtctgaat catctggcat cttccatatt
    13801 ggctgatcat cagaaaattg aaaaagaaat atgcaaaaac tttacgacgc ccctggcttc
    13861 cgcgctatcc aggggctgtg tcgaaaaagg actggatgtg aaccagggcg gatgcgagtt
    13921 caacacgagc ggtatccagg ccgtaggcat cacggacgcc gcggattcgc tgcacgcact
    13981 caacgaagtg gtcttcaaga aaaagatgta ttcgttgatg gacgtcatcc atgccatcga
    14041 taacgacttc gaaggggatg atggtcaaca aatacgacaa gcgctgcttg ccgtgccgaa
    14101 actgggacat gatgattccc ccgaagcggt caaatgggtc aacaagacgc tggagattta
    14161 ttgcaatgcc ctgaggcagg tgccgaattc gccgcgcaac ggattctata ccgccggata
    14221 ctatgcgctg aacgtcagcg atgtttacgg caagaaaaca ccgtcgttgc cgtccggccg
    14281 gctcaaaggg gttcccctgg ccaacagtat ttctccgcac tacggcatgc agatgtccga
    14341 tcttctctcc tcactgaatt ccgtgtccgg tgttgatttt gtaaagtatg cgcccaacgg
    14401 aaccaccgtc acctttacca tggactcggc tctttttcag gggagagacg gcataacaaa
    14461 tcttgccggt attttcagca cgtatttcaa aaaaggaggc atgcagtttc agcccaacgt
    14521 catcaaccgg gaaatgctgc tggatgctta caaccatccg gagaaatatc catacctgct
    14581 ggtacggatt gcgggatact gcgcatactt caaccatttg tccgatgatt tgaaaaaagt
    14641 cattattaac agaacctgtt attcatgaac aggagctgct gaaaagggct tgttcccttc
    14701 cggaagtgaa acgcaaacga gtacatggac ctgtttgcga tcccggtcat aggtgcaagt
    14761 ttggtttcat gatgccctcg agccgtaatc tttggcgctc gaaatcttcc tcgctctctc
    14821 ccgatgccag atccagcatg tccccgaaac gcagggtaac gtgcgcaaaa ggcttgggca
    14881 gcatgaaatg atcccagctg ttgaaatacc atgcacgatc ggcggagata tagaacggca
    14941 cgacgagcgc tccgctggct tgtgccagtt taacaacgcc tgctttcacg atgcctgctg
    15001 ggcctctggg tccgtccaca acgtgaccgg caaaacggga ttgaatcaga taatcaatca
    15061 ttcccctgag cgcacggctc ccgtcttttg atgatgatcc ccgcacggta tgccagcctg
    15121 ttttctcggc aatcctggcg atgatgttac cgtctttact ctggctgatc atcagtgcgg
    15181 gatgataggg ggcataggtt ttgaaatggc ggatggctga aaaaaactgc tggtgccagc
    15241 agcaaatcag aatacgtcct ccctgtttga ggtgatccat ccagggtttt tcatttacaa
    15301 cttgcaggcg gaatgtccaa ctgtaacccc gtataaaata ataaagaaaa tcctggagaa
    15361 aatcggttaa tacatatcga tattttttca gcatatctat gtcctttcag gttattggta
    15421 aaatcaaccg gtcatgaacg gccggtttgg gaatcgtctg actccggtgt tttttatgat
    15481 ggcgagacaa aagcccgtcg tggccggctg aaacatccca tgcgcgattc ggagacatta
    15541 tggattccca ttccggtggt tgtctacggc cttgcagacc gcgttattaa gtttttcatt
    15601 aattgaattc ggaatgatat catgccatcc gtcattggtt tccggtacgg aaaaaagcaa
    15661 cgtgtctttt tcatcataaa tatttatgga aacaattcta tatttgccct gcaggcagtc
    15721 caattccagc aatatcgatt cctgattcaa cacagcaggg tttcccggcg ccttttcctg
    15781 ctttagcagc atggcataag cgtccgctct gccccgttcg ttgtaaatgg ttacggtcca
    15841 gacctgatag atatgatcac tgatttttct gatgctgttc ttgtcataat agccggtgcc
    15901 ccggggaagc agtcctctga atccccagtc cgccgcaaga ctcccgtatg ccgataaaag
    15961 caatgcggct aaaataacga tgcaataaat ctttatggtt ttcatggttc gatgcaataa
    16021 aaaaatgttc cggatgtttt tcgggagaat atttttttat acttttaaat gcaaggagtt
    16081 tttgaggtcg ctaacgccag tggaacagtt tggggctgat ttcgctgcgt ttcttaacgc
    16141 cgagggtatg aaaaattttt ttcagatgat cttttacggt gtattccgtg atgcagaatc
    16201 ttcctgcaat ttcccggttg gagtacccgg tcgccgccag gaaagcgatc tctttctggc
    16261 ggggggtcag atcgagtatt gtgagtttct ccctgatgca tatggtatcc ggcattcttt
    16321 ccatgacgat cagtttcagg ggagaccgcc ggtggttgtt aatattttta acatgataat
    16381 atttggcccc gatgctgagg taggtggaat cattttccgc cagtgtgcga agcaggttat
    16441 ccagcgaatg agtttttaaa accattttgg cctcttcgtt catgtaacat aaagagggct
    16501 gtttcttttg atggtttctt attaaaacaa cgccggcgcc cttaatggaa ttcaaggatt
    16561 tgatcagtgc gtcttcatga cttatctttg cggccggatt gagatcggat atatccgttc
    16621 caaccgtcag ccagtatacc tgatttttta tctcaacggg gagggtgtta accaatgcgc
    16681 atcgcagatc gcctttttta tccctgacgg tgatggtaat atttctggca aaaccttttt
    16741 cattaatctc ttccagaatt ttcaatcttt ccagactggt ggcgtatgcg atgtcagcgg
    16801 ctgtgttccc gatgacttct tcgcgtcgcc gtccgaaaaa ttccgtgaaa gattcgttga
    16861 cgtcaacata cattccatct gtagcccggg taatggacat gggattgaga gactgccaga
    16921 ataatttatt cgtgaactct ttcggaatat ttatctctgt ttcttctttc atgttctcat
    16981 atttctgcaa tatccggatc atgctgttat taaattaatt aaattattaa tatcttttcg
    17041 attatcttta attatgctgc taaattcaat ggcgattacg ctgtaattgt taattataaa
    17101 agatgtagat ataatactta tatttttcaa ttcttgtttt caggatagtt ttgaggcgcg
    17161 taagaattgc aataatcctt cagtaagtct ctgcatatca acggcagaaa tcatcgtatt
    17221 tttatctgca aggtcaggag cgaaaattga tacaggtaag gcagtatgca ttatgtgtta
    17281 tgtcttatca aaacatatat ttattttatt ttttcgtcgt gatatacttc aaaaatattt
    17341 tataaactta tttagtcatt tattgtgaaa ggagtctgtc atgaaaaaga gaatcatggt
    17401 tttattaagc attatcgtat ttgtcctcgg gctcgtctgc attcagggtt gccagcagaa
    17461 tgaaaatcca aaatttcaga atgaatatca ggcggtttta cttaccacgg gtcaggtttt
    17521 tttcggaaag gttgaatttg tgggtacaga ttatgttctt ctcaaagacg ttttctatat
    17581 tcgcagcgtc gctaatccgg atacaaagca ggttagcaat accctgataa gaaaaggcaa
    17641 tgaaatacac ggggcggatc agacttacat taatgcccgt cacatcatta tgattgagcc
    17701 ggtatccccg caatcacctc tcgccggccg gatcaaggaa ctacagtccc agaaaccgca
    17761 ggggcaaccg taagttttta accgcggttc cagcactatt gttataacag gtgtcagggt
    17821 gcggtaatta tgataaaaaa gcccgctgca atcggcagcg ggctttttta taaaacacaa
    17881 ccttttttac ttcaacccgg cggcctggcg aagcgcatcc acccggtctg ttttctccca
    17941 ggggaagcct ttgcgtccga aatggccgta gttacaggct ttgcgatagc tccaaccctt
    18001 cggcgtcgtg agtccaagat ccttaatcat catggccgga cggaaatcga aaatttttcc
    18061 ttcagtgaga attttttcga tcttattttc aggaattacg ctggtaccgt acgtgcagac
    18121 attaatgctg agcggtttgg ccacgccgat tgcgtatgcg atttgaattt cgcatttgtc
    18181 ggccagtctt gccgcgacga tatttttagc ggcgtagcgt gcgtagtaag cggcagaacg
    18241 atccactttg gaaggatcct tacctgaaaa cgcgccgccg ccgtgagaac cgaccccgcc
    18301 gtaagaatcc acgataattt ttcgtccggt gacaccggaa tcgccggccg gtccgccgat
    18361 tacaaatcgt cccgtcggat tgatgaaaaa ttcagtcttg ttggtcagca tgcccgtctt
    18421 gtcgagaacc tgatgtgcga cgtcaatcag gtctttgcga attcttttca gcggaacgtc
    18481 cagcgtttga tgcgagatga cgacggttgt gattttcacc gcctttccgt cttcatgaag
    18541 aacgctgacc tgggattttg aatccggccg caggtaatct atcacatttg ctttacgcaa
    18601 aatggtaagt tcttccaata agagatgtgc ataataaatc ggcgcaggca tcatttgcgg
    18661 cgtggcatat gacgcataac cgaacatcat gccctgatcg ccggcaccct gattttcagg
    18721 tcgatcccga tcaacgccct gtttaatatc gggcgactga ccgtgaatgg cggaaaggta
    18781 attaaatgta tcccaactga agttttcaca gggatcatca taaccgatgt cctggacaac
    18841 ttgtcgcgaa atggcctgag gattgatctt gttccagttt ttacaggtga tttccccaag
    18901 attaacgaca agattgggcc cgacagccgt ctcacagccg acatgggcgt gtttgtcctg
    18961 ttccaggcag gcatccagta tggcatcgga aatctgatcg cataccttgt ccgggtgtcc
    19021 ttctgaaacg gattccgaag tgtaaatgtg acgtgtgtgc agtttactca tgtttttttg
    19081 taacctctct tttctatttt atttttttaa tcaaatatga ttgggcctca taaaaaacaa
    19141 aaagcccgcg ccaaagggca acgggctcat tttcattcac accagccttt agcacatttt
    19201 tagagcggag caattaactt gattaaatca ccgcttgtcg ttaacatact catccattga
    19261 aatttgtcaa caattatttc tacttttttc ttttcccgct gtttttgggg aatcggtata
    19321 aaaaatcatc aatcattttg gtcatgcaca cgatcctgtg ttcccacaaa aaacgaaaca
    19381 gacgtttata gattgatatc acagggattg ttttgtagca ccatgaaaag gaccggcttt
    19441 gtttttctgc aaaaacaaag ccggtgtcat aaaccatctt ggggtgaaag aaatcaacat
    19501 gacgcggttg gccgttgtca gtgttgatga attacgtcgt ccggctatcg tcggtcctct
    19561 ctgacgggac gtcggtcatc acggtatttg atgacggtct ttgatttttt tacttcacgc
    19621 ctgtattgcg tgccggagcg ccggtctttc cagtttttta caccccagct atgtttttcc
    19681 cagcgttttt cttttttcca gccgctccag ttacgttgaa gctcgtgatg gcttattttt
    19741 ctgtgttccc atcggtgtcc tttccaggtc ttattgcggt aattgtcctt ccacccgggc
    19801 ggaatggtac gatggaaaaa cggcggctga tggtcataag tccatcccct gtcgtaatag
    19861 cgtgaacggt accagcggcc gttccacggc cgccaccacc aaccggaata gaagaataaa
    19921 tcgtcctgaa tatcgggtac cgcatagatg tatgtttccg gtatcaccac cagatgcggc
    19981 ggcgccggga aaatgattgg cggcggtaga gggatatcga tgtgcacact gactcccgcc
    20041 gatactgaaa acggcgcaaa gagcacgcct gcgaaaaata tgattccgga cattaatttt
    20101 ttcatcggat tacctcctcc gtcgggtcgg ttaaggttgg tcgaaaagtt tccatcgtaa
    20161 gtctgatgcc ccaacgataa aaaaaagaca ggggctgtca agcatgaagc aacttcctgc
    20221 cgcccggttc ccgagccatg tttaccggtc attcgaccat tatgatcctt gctttgcaag
    20281 ccttttccga aactttaaat aatcgtcggc agcgattttc ggttgctcta tcatcggcag
    20341 atgtcccagc cccttcatga tgatgatttc ggagttctgc aataatttgt ttagaatcgg
    20401 cgccgtttgg ggactgatga cgcgatcttt ttctccccag acaatcaggg ttggcgtttt
    20461 gatttctttg acgtatcttt ccgatgcatc ggacgacaac tgcttgaaaa tacgctgttc
    20521 cagctcataa ttcctgatgc gttcctgcgc catgacgttt aaaatcggtc gcggaatgaa
    20581 cggatgatcg gtcatgacaa aactgaatat ttcagcaaac tcatcttcgt ttttggccat
    20641 cagcggattt ttgcctgttt tggcgatgat ttcatgcaat tcaccgggcg gagcgctcca
    20701 gattccgccg ggatcgatca accacaagct ttttacttca tcaggataaa gaaaagcgta
    20761 catcattgaa atgtggccgc ccatggaact tccgcccacg tgcacatctt tgatccccag
    20821 cgcctgcgcc agggttctga ttctgacagc ctgcgccagt gatccgtagt cttcattttg
    20881 cggacggcta gaatcaccga aaccgatgtg gtcgggaatg atgaccctgt aatgaggcgt
    20941 cagaaacctg gccacccgcg tgaaattatc cttgttcgcg ccgaatccgt gcagcagcat
    21001 cagcggctca ccttttcccc cttccagata aacgtaacgg atatgatccg aaaggttgat
    21061 ttcctttttt tccagcccgg aacgtttgcg ttccacatcc agagcgagcg acgtcgcctg
    21121 ttcgggagcc agataaacga atcctatgcc cgtcgccacg caaagaatga tcaatacaac
    21181 aatcgccttg agtagtcgca ttttcccctc ctgttttttg atcggattaa ttctggttat
    21241 ctaatatagc aattattggt caagatttaa aatgaattct tatccgtttt ctgcggatag
    21301 cgatttctct tttgccgtgt ttttcagact gccggtataa agcgcgtatt caagcagacg
    21361 gcattcgata tcgccgttgt acaggatcac acgccttttt gttctcaagc cgactttctt
    21421 gatcagttcc agattgccgg taaaaatata tccatgacaa ccagtacatg tttttttgaa
    21481 gaaatcgccg atgccctgat aaagcgcttc cagttttttc atctctcttc cccgatggcg
    21541 gttgccgtcg ccggacttgt cgccggattt acgaagaatg attttgcgac cggggccggg
    21601 cgtttctctg ctgtgacgga aatccgctga caccggtttg cgaggatcgg aactcatgcg
    21661 ttcaccgtaa ggaggattaa aaataatgat gccgtcgccc gcggggacgg acgttttttc
    21721 gaacggacaa atattgaatt caatgaaatg atccaccccg gcagtctgcg cgttttgtct
    21781 ggctgccgaa atcgcctctc ggttgatatc agtggcgata attctggccg gcaatttttt
    21841 cagggcattc gtccgggctt tgctacgcag cgtctgccag tgatcctcgg ggaacccttt
    21901 gatgaacata aaaccgaagt gatttctcag cagccccggt gcccgatgca gcgcaatgga
    21961 tgcggcttct atggccagag ttccgctgcc gcacatggga ttgataaaat tactttctcc
    22021 cttccagcct gccgccataa tgaccgccgc cgcaagcgtc tcctgcatgg gcgccacgag
    22081 gggtaccttg cggtatccgc gcaatgccag tcgttctccc gacgtatcca ggtaaacagt
    22141 ggcccggtta tctttccagt atacatgaac cacggccctg tttttttccg gaccggaatc
    22201 cggccgattt ccggaaatgt cgcgtaggcg atccacgata gcgtctttga ccttgacatt
    22261 gacaaattgc gaatctttaa tgagcgggtt ctcggcgatg gaggtcacgc agagatacgc
    22321 cgcgggtccg gaatcatgga gcagattttc ccagggcagg gcatgaattt ttttgtagag
    22381 ccattccggc gtatcagcgg agaaggagtc caattgatat aaaacccgct gggcggtgcg
    22441 cagatgcagg ttgagcgcca tcgtgtcctg aagggttcct tccgtttgaa tggccgtatc
    22501 ccattctgcc cgaaccggat aattcaaggc ctggacctct tcttttaaaa aaggcgatac
    22561 gcctttcggg caagtgacca caatggtatt tttcttattc cagatggaca tgaactgctt
    22621 ctagcacagg tcgttgtttt tgataagcga tatcctgtaa cgatttttaa cgcggagggt
    22681 caaaccggaa aaggaaccgg gatgatcaca gatatttatt tctaagaaaa agatttagtg
    22741 ataagagagc ggccgggagc gtgattgccg gtgatgcagg attttattga tgaaaaatga
    22801 tcgcaaaata ttctggggct ggtacattgt gggcggcgct tttctggtca tggccatgaa
    22861 ttacggcgcg cgatactgtt tcggtgtttt tctcaagccg atggccaatg aattcgcttt
    22921 atcacggtcc gtcatatccc tggcggcggc catcaacatg atggtttatt cattcggatc
    22981 tgtttttgtc ggcagaatgc tggaccgcgt tgcgccgcgc tggatcacaa cggccggcgc
    23041 cggcgttgcc gcctgcggtt acctgctgac cggttttgtc gatacaccga tctggctgta
    23101 tatcagctac ggcgtcatgg tcgggttggg gtccgccgga atgggcgtcg ttgcctgcag
    23161 ctcttccgtc agcaagtggt tcatcagcaa gcggggtgtg gccgtcggca tcgcatccat
    23221 gggaatcagt ctgggaacgg tcatgctgac gccgcttgcg ggttatattg caacagtatt
    23281 cagttggcgg ttcggattga tggcgttgag tttcatcatg ctgattgtgg gcgttgttgt
    23341 tgcgcagacg ctgatgagaa gaacccaccc ggaagcctac ggtctcttgc cggacggcaa
    23401 tacaatatca ggcatgcctc aggcggcaga ggcaacggcc gtttgtccag tgtccaccat
    23461 gatccttttt cgggattccc gtttctggac gctggccgtc tgtcagggat tggccgttat
    23521 gatcagcatg gccgtttttg ttcatcaggt agcatatgcc acagacaacg gtatttccaa
    23581 aatggcggca gcctcgtctc tggcagccgt cagcctgacc ggttttatgg gacaattttt
    23641 tttcggctgg ataaccgaca ggatcaatga ccccaaatac gcttctttcc tcggcatctc
    23701 ctttatgctg gcgggaacgg tcattctcct gaacgtggaa agcctgctgc atctttacgt
    23761 tgcggctctt gtctacgggt tcggatacgg atcgcttgcg cccatgctgc ctctgattat
    23821 tgccgaccga ttcggccggc agtgcatggg atcgatttac ggcctgctga cgttctttat
    23881 cggcaccggc ggcgccatcg gtccggttct gggaggtttt atttacgacc atttcggatc
    23941 gtatcataac ctgtggctga tgaatattgt cgtgctgagt cttattgcaa tggcttttct
    24001 gacccttaaa aagggcaggc cctacgccgc ttgatcaaat gaacagaggg tctagcgggc
    24061 gagtagaagg attttcctga tgacgtcttt cgtgtagcct tcatagcacc atacttgcga
    24121 gagggcgaaa gcattcccgg cgattgcgtc aatatcgctt tcggggatgt caactgcctg
    24181 taaagacacc ggactgccga tcgattcaaa ccagtgcttc aggcaggcaa ttcccttttt
    24241 tgctgcggat atcgcgccgc gtcccttcac atgaaaaatt tcccgggcaa gtttttccaa
    24301 ccggtctgtt tttttgtcca acgcgtacga catccatgcc ggtaaaataa tggacagtcc
    24361 ggcgccgtgg gctatatcat aaatggcact gagcgaatgc tcgatcatat gcgcgggcat
    24421 tcgcacctgg cccattcccg ccgttgtgag cccgttaaat gcaagaccgg cgctccacat
    24481 catattggct cgggcgtcat agttatccgg ctccttcaaa atgatttccg tgctttccat
    24541 gacggttttt atcagcgatt cgaccagcct gtcctgcagc gggctggcgg aagaggtgtt
    24601 gttgaaatat ccttccagca tgtgcgtaat gatatcgacg gcgctgtaag cgctgtaggc
    24661 ggggctgagt gaaaaaagaa cggtcggatc cagtacggag accttcggtt gaataaaggg
    24721 agatacgatg cagtattttt gacagccgtc ttcccgggtg acgacagcgc cggcattcat
    24781 ttcggatgca ctggccgaga cggttgccac cgtcagaacg ggaagggcgc cggtgacggg
    24841 cttgctgaac gtaaaaaaat cccacacatc gtgatgtgcc acaacccctg cggcaatagc
    24901 tttcgcagca tcgatcacac tgccgccgcc gaccgccaga ataacatcga cgttttcccc
    24961 gcgcgccagt gcaatgccct tttgcacatg cgaaagcacc gggttggatt tgacgccagg
    25021 aaaatcaacg atttcgacgc cggctttctc aagagcgttt gttacctgat cataaacgcc
    25081 gtttttcaga atcgtaccca tgccgaaaac cagcagggcc ctgcgtccaa aacgggctgt
    25141 ttctttgccg attcggggaa tctgaccctt gccgaatata attttggtcg gattttcaaa
    25201 aacaaaattt tgcatgattt taccccttcc tacgtgcttt tgcggtccgg ccagatccta
    25261 tcgccggcct ttgagacttc ttctacaaga aggcgcccat ggttagcgca ctgatgtcca
    25321 tgctgaaatc gagctttgtg tggacgagtt ctctgttgcg gaccgcaaac ggatgaagaa
    25381 ccagagaata atgttccagc gtcaatttaa gcgacgagag catatctgcc agatgtcttt
    25441 tatcagccat taaactgcca agcgtgacaa ggcagctttc gaatgcttcg ctcacgggca
    25501 gggtgacctg atgaatattt tttaccgggc aatccggcgt taaatcgtca ggcgcgggcg
    25561 ttacggttaa ttgccggcac cagcgcagaa acaaggcggg attgagcttg aaagcgggag
    25621 caaaaaagta aaaaggtgtt ttgtcccaga tgtccagggg ggctttcggc agattggcca
    25681 ctttaatgaa gtctgcgtag tttcccagcg cgataccgtc gaccttcacc ttcagttgcc
    25741 agaaagggag gtaagtgatc tcgtcccctt tttcccgcca cgttgcgtac ggaatcggaa
    25801 caaaatgctt tttcgatgga ttccacgcac gatggcaatt ctcacaaaat acaatcaagg
    25861 cgtctttggc gccgggcaga tccgcgccgc attcgggaca aagcaggggc agaaaatcaa
    25921 catgccactt ttcgtccggt gatgaaacga gtttgtcctc catctccggc tccttttttt
    25981 gtacgtccag aggcttcttt aaaacggcat cgtaaagacg gccgttggca aaataaaccg
    26041 gcgtatagat gatgctggcc gtttcgccga tgaatatttc ctgaacggcg ccggcctgtg
    26101 ttttgaaatt tttcaacagt tttcccctgg tcttttgaga cgctgcaatg aattttcctt
    26161 catgatctgc attgcccaca aatcgtaatt tcatcgcctg aggtctgagc cccagcgagt
    26221 gggggaaaaa aggcaaatca aacgagatga aattggcgtc aaaatattta taagcaatat
    26281 tgtgcgttgt gatggcgtag gagagaccct tgagacgcca gtaggggata aaataaattt
    26341 tttcagaaat tccggcggcc ggggggatgt aaaagagaaa gtgatcatcg gtggccagat
    26401 agacacgcgt cctgcagaaa tggcacgaaa gaatacggtc cgtctcgtcg agaataatcg
    26461 gcgcaccgca ctggggacat tgctgttcga tctgaaagtt agagccgttc accgcactta
    26521 ttgcaataca tcgaatccgg caggttttca aaaccgcact tggcgcactg tttgccggga
    26581 ggatttacag tgaccggttt gccgcaactg acgcagaaat tggcattcac aggcaagttt
    26641 cttccgcaat gtgcgcattt gttgacaatc agaatctgat gaccgcatgc gggacagaaa
    26701 tgcgcttcct tcggcacaac ctgtctgcaa tccggacagg tgaaaacgtc ggcagcaacg
    26761 gttgttccgg ctcctttgaa cgcttccagc agcattccgg gcatcatgaa acccatgccc
    26821 atacccaatc cggcgccggc ttccgactgg ttttgcgctg ctgcttcaac ggccatcgcc
    26881 gctttcattt ttaaaagctt gttgaggtca tcaaagacac ccaggcggct tttatcgtcg
    26941 atggcttttt ggacttccgg tggcggggtg atggaattga tgtacaggtc ggtcagtgcc
    27001 agaccgaatt tgctcagatc cttttgcagc atgaccttca gaccttcgga aaactcattg
    27061 tattttcccg gcagattgaa aatcgtgtcc accttttcgc ccatataatc gttgaagcgt
    27121 gaaacaatga cacggttcag atattcttcg atttcttccg tgctgaaaat gccttgtgta
    27181 cctaccaggg agttaatcag cagaagcggc tggatgacac ggatgttgaa aacgccgaaa
    27241 gcacgcaggc ggatcagccc cagttcccgg tcgcggaaag ccaccgggtc ccgtgtgccc
    27301 cattttaagt tggggaatac cttcatgttg atgaagtaga cttcggcgcg cagcggactg
    27361 gtcattcccc agggaatggc aatgactttg gtcagcagag gaatgttggc ggttttcagt
    27421 gtgtgtcgtc cgggaccgaa ggcgtcatag gccttgccct tgtagaaaaa gacggcgctc
    27481 tgactttctc ggactgtcaa ttgggcgccg aattttattt cgccggagcc cttttccgga
    27541 atcctgtgta cgatttcttt tcccgattcg tcaaaccatt cgatgacttc cagaaaaatt
    27601 atgttgtttg ttcccatgtc aatccgcatc ctttattttg tggcgattat tgaaattcaa
    27661 cgtttcagcg tgtaaaaaat cataagggag ggcaaccaaa tagtcaagaa caaatgaagg
    27721 attcaatttg attttttaaa cgggatatta tagagaaatc atcatgaaaa aaacgtctgc
    27781 tgagcaaaat atatatgacg gtcttgcgct tattgccgat ccgattcatc agtatattct
    27841 ttttaccgtt ccaaaggaca agtctgccaa gtctgccgga gaaataacgg agcagcatat
    27901 cattgattct ccctggatgc agaggctcag aagaatttat cagttacaga gcgcccgctg
    27961 ggtttatccg gcggcggagc attcccgttt tcagcattcc ctgggcacga tgcacatggc
    28021 gggtgaattt gccaaaagca tttatccgag cctgtcttcc gtctgcaaag atgttccgtc
    28081 ctgtcattac gtggaagaat tactccggct ggccgggctt ctgcatgatg tcggacacgg
    28141 ccctttcggc cacttttttg acgataattt tttgagcaac tggagtctga cccatgaaat
    28201 cgtggggcag aacattatcg tggacaagct cggcgatgat cttgcgcgcg tccgtcgaag
    28261 cccttccggc ggattttcac gcacagaaag aatggatgcg caaaaagtgg cgtatttgat
    28321 caggatgccc gaagacggtg aggagggcag gccgccgcgc tggctgcaaa tgctcagaca
    28381 attgttttcg ggtgtttata cagcggataa tctcgattat gtgcagcgag acgcttacat
    28441 gaccggattc tccctggata tggtggatat cccccgttta cgctactaca ctttttttac
    28501 ccgggaaggt ctcacgcttc atcaatcggg catttccgca ctcagacggt tcctcaacgc
    28561 ccgtctcaat ctctacacca acgtttattt tcaccgcacg acacgggggc tggatctgca
    28621 tcttcaggaa ttttttcatg ataccatgca gatgatttta cccgccaatc cgctcaacaa
    28681 tcttgacgat tatctgcaat gcgatgaatg gacgctgttt aatcatgtgc agaactggct
    28741 gcgatccaaa gacgaacaaa agaaaaaact ggctgccgaa tggaaaaaac ttcacgaccg
    28801 cgaagtgaaa tggaaaatgt cttttgttgc agaaatttca ctggatcaga tacagcccgg
    28861 ggttggcttt tcccgggcag ccgattatga acaacagatc agggaacgcc ttccgaaacc
    28921 gctgaaatcc cttcctttca aggttgatct ggcgacgcag gatccgcgtc cgctcaatcc
    28981 gattaccgag agggaaaaga gaatcaatat ttttaatccc gtcacacaca agacgtctcc
    29041 cgaaccgctt ctcgaaattt accgttttat tccggcacgg gtcatgcaat tcagagtttt
    29101 tgctctgaac catgatcatg acggagacct ggccgctgcc gctgaaaaag ttcttggaag
    29161 ctctgcaaaa tcaacactga cgaatatctg acatgagtaa aaacgacgag acaacagacc
    29221 tgactctttt tgtgcggaca acgggagagg aaatcgttct ctgggaccgt cagcgtattg
    29281 tggatgccct ggtgcgcgaa gccgatatcg atgatgacgt ggcaggccag atttcgaaag
    29341 aagtggaaaa acagatcatt gtatcgggaa tcggcctgct cacaacggcc ctgatccgtg
    29401 aactggttaa tgtccggctg atcgaaagag gcttggagaa agagcgccgg cttcacggcc
    29461 gtctgggatt ccccctttat gatgtgcgcc agttgatcct gcatcccaac aaacaggacg
    29521 ccaatatccc gcattctccc gaaggcacga atctgatttt tgccgaaggc attaaacggg
    29581 aactctcttt gtttgacgtt ttttccgccg atgtcggcga agcccatgcc acgggagata
    29641 ttcatatcca cgctctgggg tatattgacc gcccgtatag ctgctgccag tctctggaat
    29701 acctcaaaat caacgggtta aatctgcctc atgcgatcaa ttccgccaat cccgccaaat
    29761 acgccgaagt gcttctggca catatggtac gcttcagcgc tgttttgcag ggtcattttg
    29821 ccggaacggt cggctgggat gcggtcaata tttcatttgc gccgtatctg gcctccatga
    29881 ccgacaagga agtcaaacag tttgcccaga tgctcattta tgaattctcg cagctcgccg
    29941 caacgcgcgg cggacaggcg ctctacaccg acatacacat ttattatgat gtcccgccgc
    30001 actgggcgga ccttcccgcg gtgggagcgg aaggaaaacc gacgggaaaa acatacggcg
    30061 attacacgcg ggacgcccag cgctttgcca tggccattct ggaaattttt gccgaagggg
    30121 acgcccgagg aaaaccgttt attctgcccc gtcccctttt gcatattacg gatcatgtct
    30181 ggaaaaacga taaggcccgt gaatttctgc ttcgtgcctg cgatgtggcc gcgctgatgg
    30241 ggaatccctg ttttgttttt gacagggatg aaaaagcggt cgacctgatt ttcaacgacg
    30301 aggagaggcg caaaccctgg cttcagcgcc gggccgctat tcagacggta acgctgaatc
    30361 tgccgcggct gggttacaaa gccggtggag acgaaaaaga agtcttttcc ctgcttgaag
    30421 ccttcatgga gaaagccgtt aaagcccatg tgcagaaaaa agattttctg gaaaaattgc
    30481 tttcctatgc cgaagaggga cctctggccg tactggccat gaaccacgac ggatttccct
    30541 ttttaaggat gcatctggcc agctatgtta tcggcattgt cggtttgaat gaactggtgc
    30601 agattgttac cggaaatgaa atgcatcaaa aacaggaggc gttggaattc ggcctgagag
    30661 ttgttgattt catccggcag gaagcgcttc gtttaagcag acaatgcaaa atgaaattcg
    30721 ttttggggca gtctctggcg gaaaccaccg cgtatcgttt tgcccgtctt gatttaagat
    30781 atttctctcc gacagccgga cgttatgtca agggagatat tgccgaggga gccatttatt
    30841 acacgaattc gacgcatttg aatgttgcag ccgatgttgg gcccctgcag aaggtgatgc
    30901 gggaggggct tttccatcat taccttgaag acgaggtgat tacgcatttg cagctgggag
    30961 atttgaatcc gggaaggaac gtccttgccc ggttcatccg cgacgtattc tatcattccg
    31021 caaaccgcca gatagatttc aatccggaat tcacgttttg tctttcctgc aaggaaacga
    31081 ccagagggtt gcaggacaaa tgtgccgcct gcggttctgc cgatgtggag ggcatcgcga
    31141 ggctcacgaa gtatttcagc aaaacctccg gctggaacaa ggggaaactc gccgagctga
    31201 aagacagaac aatgaatgac aactttaata atgtcgatca gccgaaatgt taagacgaag
    31261 ctggattgtc cgcggcatct cgacagtttt ttcttaatac aaaaagcatg tatttctttt
    31321 catttctaaa ttttatcggg tcaacggccg ccagttcctt ccatatttta tgatcaacgg
    31381 attgctttaa atctctgatg aggtagtata accatgcctt tttacgcttg atttttccgg
    31441 atgtaaaatg gaccaggggt aaaagaaatc gttcagcata attatagaca aatttcagcg
    31501 tcggcaaaac atgaggcgtc atgtcctgct gatacgtaat ctcccagcca tgttcctttg
    31561 atttccgaac aaaatcatcg agcaggtgtg atgacttgtt gacgcctgaa ttattaaatt
    31621 taaaataatc cgcaataatc catgtgccgt caggtttgac aatgctttcc gctttttcga
    31681 acgctttctc aagcggtata tattgaagcg attccgaatt gatgatggtt ccatactttt
    31741 tatcggtttc gaattcttga aatcgcttct gataacacgt aatcgtcggg tactttgatt
    31801 ggatatgcct gatctgattt ttattcggag tcaacgcctc aacattaaat cccttctggg
    31861 atattaagtt cgacaagcca cccattccgc agccgatgtc aagaattaaa tcttgcgggt
    31921 tgatgatttg atcgatgatg ttctgcgcat atctgacctg ggctttttca atttcagcga
    31981 tagagatggt gtcgacttca atctcggtat tttcgaaata cccataatga agcatgtcat
    32041 ttttgaatat tttcgaataa agatgaagtt ccaggtcgga agttgagttg ctgaaggttg
    32101 aatttttctt gtggtaaagc caggcgttaa aaagaacaac aggatttata atttttaaaa
    32161 gtattttctt cattatttgc gggagggact atatgggaaa cattattcta tgtcaatgac
    32221 aaactaaccg acgaaagtcg gcgattgaat tga
//
LOCUS       DAONQZ010000023        31468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1566_length_31468_cov_17.019323, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000023 DAONQZ010000000
VERSION     DAONQZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 31468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31468
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_31468_cov_17.019323"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1490)
                     /locus_tag="PLR13_05675"
     CDS             complement(225..1490)
                     /locus_tag="PLR13_05675"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HQC18859.1"
                     /translation="MAERDMVVMSLGELKRLKLIQSAIDRQISQKQVSGILRLSERQI
                     RRLVRAVREEGDKGIINRSRGRPSNRGLSAGTKENVIALYKEKYSDFGPTFATEKLWE
                     RDGIRLSDETLRNWLIGAGLWKKHRKRSAYRQWRPRRSCFGEMVQMDGSHHDWLEGRG
                     PKLVLMGYIDDATNTIYAKFYDYEGTLPAMDSFKGYVKKYGLPMSVYLDRHTTYKSSR
                     KPTEWDEASDLKFLSQFERVLKELGVEVIHALSPQAKGRVERLFGVLQDRLVKEMRLH
                     GIKTKDEANGFLETYLPQYNEKFRVCPANETDVHVRPPRHFNWDKYLCIKDQRTIRND
                     HTIAYNRKLYQLEENDSKKVHIEKRRDGSLLIISKGRALKYKEITERPKQMVAVKKDL
                     RAYNRPPKPSKDHPWKQRWKTWGTTPTVL"
     gene            1697..3148
                     /locus_tag="PLR13_05680"
     CDS             1697..3148
                     /locus_tag="PLR13_05680"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HQC18860.1"
                     /translation="MGLKTADQYEESLRKMNLKVYLLGEQVKCPADHPIIRPSMNSVK
                     MTYALAQSPEHEDLMTAKSHLTGEKINRFCHLHQSTEDLIKKVKMQRLLGQKTGACFQ
                     RCVGMDAINAVDSVTFEMDKKLGTQYHDRFIRFLKMVQEEDLVVDGAMTDPKGDRSLP
                     PSRQTDPDLYTHVVEKRKDGIIVRGAKAHQTGAVNSHWILVMPTIAMTKDDADYALSF
                     VAPADADGISYIYGRQSCDTRKLEGCEIDVGNRQFGGHEALMVFDNLFVPWENVFMCG
                     EYEFSGLLVERFAGYHRQSYGGCKVGVGDVLIGAAALAADYNGAAKASHVKDKLIEMT
                     HLNETLFSCGIACSAQGHRTASGTYLIDLLLANVCKQNVTRFPYEIARLAEDITGGLM
                     VTMASEKDLRHPEIGKIVEKYFKGIASVPTEDRCRIMRLVENITLGTAAVGYRTESMH
                     GAGSPQAQRIMIARQGNLEHKKKLAKEIAGIKD"
     gene            3190..3480
                     /locus_tag="PLR13_05685"
     CDS             3190..3480
                     /locus_tag="PLR13_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18861.1"
                     /translation="MIGFKYCGGCNPVINRAQLVQDIQRRLSAEFTLATDQSPTQWDI
                     GILVCGCLSACADKPDFRNLARRWIIIAGNSVDYGDAPEKDLAEIVLNKLKL"
     gene            3787..4488
                     /locus_tag="PLR13_05690"
     CDS             3787..4488
                     /locus_tag="PLR13_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC18862.1"
                     /translation="MTDKNRPKTRKDRIMDAALRIFAEKGFQNATITEISKAAGVSEA
                     TIYEYFGTKEDLLFAIPEKITNDTFEESSKVIPYIKGVEGKIRAILLFYVKLYQSNPN
                     YSALVLLQLMSNRRFRQTPAHAAIRTSAHTLLDCIREGIADGTFKKDSNPYLIRSVFM
                     GTIEHLFIHWHMQGMPKKKPGIAEMLDALLETVFDGIRARKEDSGTTLYLKLDDAGLL
                     EKILKKGRERHQNKK"
     gene            4525..5988
                     /locus_tag="PLR13_05695"
     CDS             4525..5988
                     /locus_tag="PLR13_05695"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HQC18863.1"
                     /translation="MRTREQYIERLRKMRRNLYHDGEKIDRDDEIQAPVLNVMGLTFD
                     AVHDPELKDLCTATSHLTGETINRFCHVHQSTDDLHKKQDMTRALCRKAGMCIQRCMG
                     TDALNAVNAASFEADKVNNGNTQYHKNFLKWLERFQKEDLVGSCAQTDMKGERLKRPA
                     EQTDPDMYVHVVEKKKDGIVVRGCKLHISEASISDEIIVVPTRALGPDEKDYAVAFAV
                     PSDYEGVKQVVSIHNQHKRKHIKRGIDIGYSDSYIIFEDCFVPWERVFLCGENDHGGM
                     CALLFALFHRHSYSGCKPAIGDVFLGLAALAAEYNGIEKTPHVKEMLAEIIKVSELGY
                     AAGYTASSMGKPEVIIPGLGMCPYGPGSYIPNSIYCNVGRCLTGEAVFHEQELICRIA
                     GGLPSTFPYEGDLTHPDLKKVLEKYLNRNPKVPVEDQIKFWMTLGDYTIGTMAGVMNY
                     GNYHGGGSPIMEQIAITSQYDIKDRKKLIKRLAGIGG"
     gene            6100..6900
                     /locus_tag="PLR13_05700"
     CDS             6100..6900
                     /locus_tag="PLR13_05700"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC18864.1"
                     /translation="MLDVSQFSLKDKVAVITGGGRGIGQAIAFAFARAGAKVVVTSRK
                     APDLEATANEIKAFGGDAYPLPAHLGKSEEIKKMIDAVMAKYSRIDILVNNAGASPAK
                     GSVLECDERLWDKLMDINLKGAYFISQAAANIMVKQGGGKIINIASIDGHNPEPGLSI
                     YAISKAGIRMLTKAFASELVRYNIQVNTIAPGPIATKMLKSNWEFLPPEDAQKVKELV
                     EKALPSGRIGDPDEIAGAALYLASDASSYTTGAEIVIDGGVLLGMHPS"
     gene            6915..7454
                     /locus_tag="PLR13_05705"
     CDS             6915..7454
                     /locus_tag="PLR13_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQC18865.1"
                     /translation="MISRTAAVLVIIDIQGNLAQAMFGKENIFANAVKLIKAFKTLNL
                     PIIVTEQIPGKLGKTLPQIADEIEDFNPIAKESFSCWGEKNFKERLESLSRRHVVLLG
                     IETHVCVYQTALDLLSEGYNVHLVADAVSSRTPENRQIGIKAMKSAGAHIASTEMVLF
                     ELLRTAADPKAKEFFKIVK"
     gene            complement(7572..9503)
                     /locus_tag="PLR13_05710"
     CDS             complement(7572..9503)
                     /locus_tag="PLR13_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQC18866.1"
                     /translation="MFSAHSENESATESPGEPPSGGFNKKIIFLAFASIGVVFGDIGT
                     SPLYAIKACFYGQHALAPDITNILGVLSLIFWSMFVIVSIKYVIFLLRADNRGEGGIF
                     ALVSLLNVSKKSFSPLVQSVLVLAGILGAGLLYGDGVITPAISVLSAIEGLEVATHAA
                     APFILPLTCVVLILLFVLQRKGTSNIGKVFAPIMVLWFLSIGIFGIMQIVREPSVLRA
                     LNPLYAVEFFLNNGMHGVVVLGSVVLCITGCEALYADLGHFGRNAIRLSWFSLVFPSL
                     LCNYFGQGALLLAHPEMNVNPFYKIVPEILLYPMIGLSTAATVIASQALISGAFSLTQ
                     QAAQLGLCPRVRIIHTSSDMQGQVYIPSVNYALMMACIAVVTGFRESGALAGAYGIAV
                     TGTMIITSILYFLVLTHHRGWPLRKVIPLVGLFLLVDIAFFTGNFFKLADGGWLPLLI
                     AAIIAVAMTTWKKGRNELYRNLVGARFPLESFLSELPKRHMPRVPGTAVFMTLSPVGT
                     PPTLLHNVKHNHILHETVVLLSIITKDTPAVPAGERIKLADLGQGFYRVEAFYGFMQK
                     PNVPQLMKILAQYGLVTDPMTTTFFLGKETLLTGGKSKMMKWRKSLFAFMSRNAGNPT
                     SYFGIPANRVVELGTQIEL"
     regulatory      complement(9558..9688)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            9991..11262
                     /locus_tag="PLR13_05715"
     CDS             9991..11262
                     /locus_tag="PLR13_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HQC18867.1"
                     /translation="MAQAQVFENKQPVLGINSLGRIGKLTLWHHIGRKYFKEIIVNQG
                     REVGTGLDSVAQAIEKDSTYGLMHKFLYGVKAERIIQIVDEKEGKLLIDGIPVTILRE
                     ARNPADVPWKKYGVDVVVDCTGKFKDPTVAADHKSGSIRGHLNGGAKVVINSSPFKIK
                     NKALALPDDAVTMIYGINHTSFNPKKHLIVSAASCTTTGLAHMVKPLLENEETSNILT
                     ASMSTVHAVTNTQSVLDSVPKAGEKDLRKNRSILDSIILTSTNAAKALAEVIPQVQNI
                     GFMADSIRIPASTMSLIVLNATFQARSQGAATAINTKMLNELYGKAAANNPEKLIRFT
                     MEQNVSADLFGIDAAVIIEGQFNHTRTAFINLDLAHIPNMPAEVANSFPDKVMKVPVV
                     HAKIFGWYDNEYGSYTNRMGDLTVHIHKNIL"
     gene            complement(11341..15558)
                     /locus_tag="PLR13_05720"
     CDS             complement(11341..15558)
                     /locus_tag="PLR13_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQC18868.1"
                     /translation="MATTKDNPRKKSEELDSFALKVNLERTAATIEIPDQYAPLLKVV
                     ENHYGLQKKIYELLTELHHPFVNWEYVLKELKSVSIGDFYIYNKHQDGLSALATMLCI
                     YFEVIKSSSDKDVKDNAVHYLFDYIDTILNQSNEQTARNLLLFPEIIKSLLDAINQQE
                     IIFKKCSSYAKKIIKNFPGHGDVLQNRLFVDLLYRIFRITYQFWLEQPDPSQWFIAGE
                     GESPESVAAYGQFIRPLSHASMRMLMEKLETSYQNTHEDKNDAIKNYLEMPDYFQIVN
                     GYLQIADQLEKSKTYEGHQHITKLRFLFQVMEVPSLADIHASALREINHSLKMVFQEE
                     QQENLHDFVRKIFGFLKKSKSQREFSGAIFDCITITAREVFAQNSHELANTFISELIA
                     YGFQYPEIKGSNEEWQVKVNPAHIMNIRAWLEIISMKPRWTKKLISALVINLKMGGIF
                     IRDTDLIQKNISALLNTDIAPAYSLIKQLLRIFPVYFSEIGAEGELRDITTKVDELSM
                     RNDKLVYFFRKQSHVESNSLLVKFTEDIFQYWNTGDMSYLRSHLPVEVYEQVMNPGEY
                     FTGMHKAFKYFFTKVQSPLKFLDWDNEKVCKELANVKGINARDQERVKIMIRIYQLIY
                     KKYYPQFIDLLKDLEAVNAFPKADISSLKRSLTTKNYHRSITIILKFLSILKERILSP
                     KKTPSYENIYYKRHIAAGIPSMYGTYYERKFEAVGLSLRLETLATMLFEELSKSFNLK
                     FITKHTIRKIHTYLWLYMNALEIEGIATEGLAAKIKYVTNALSIKQFSLEQYVDIFRF
                     ISKDIQDIIRNYYIDIHTTCLPVVIGQVYRREIDSERKSGQKQHEKLIYAQSENFIRG
                     IIASAFGIQVLDNFVNSVIKTLNAESEKFRDNKQILKVVMAYNPELAVSSFYKKNKKV
                     DNQILLGNKGYFLKELVSFGFNVPPGFIITTEVFRGYEAVYGYKYIFNDLAVRVNKEI
                     KKLEKITGRKLGDRRNPLLLSIRSGATVSLPGMMKSFLNVGINETIAENLSEKDGFKW
                     AAWDSYRRFLQMWGMFKGLNRNFFDAIIDEFKQKYGVERKIQFSPEQMKQIALSYKKG
                     LTDNGIEFKEKPLDQLRDAILQVFDSWYSEQAEIYRRQMHISDKWGTAVIVQKMVFGN
                     LNDNSGSGVIFTREPKSTSSDVTLYGDFIFGVQGDDIVSGLVETYPVSEKQRIAECRD
                     SKISLEDKFPEIYAELLRISEILIYEKGFNHQEIEFTFEGPTRDKLYILQTRDMNQIT
                     TQNWRRFKDSPALQSSMIGAGIGVNGGALAGRAVYSEADIKRFRSEEPQTPLILVRPD
                     TVPDDVGVLLQVEGLLTAKGGSTSHAAVTIPQLNKVGVVGFSKLKVYETDEYAAIENQ
                     TIRAGDFISIDGWSGTVYLGRHESETE"
     gene            complement(15755..16066)
                     /locus_tag="PLR13_05725"
     CDS             complement(15755..16066)
                     /locus_tag="PLR13_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18869.1"
                     /translation="MSRAKVGDVLGCEVCGLKVILDEDCGCATIDLICCEEPMVNLGP
                     AKPKKAQAIAAPRKKTVVKKTKAAPKKKPALKKAKKPTVKKAVKKATVKKTAKKSSKK
                     K"
     gene            16340..18292
                     /gene="alr"
                     /locus_tag="PLR13_05730"
     CDS             16340..18292
                     /gene="alr"
                     /locus_tag="PLR13_05730"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQC18870.1"
                     /translation="MPHKKNQKYRSWVEVDLDNFIANLKEIKRLIGAKADFMMTVKAD
                     AYGHGAIEISNIALKCGARMLGVANADEGVQLRISGIEAPVVILGPSTTAEIEQIIKY
                     NLIPSVSDLSFAKKFNNALIKSHHKRPLHIEVDTGMGRGGAMHTEAVKLVLDLSGLSN
                     IILEGIFSHFASSEKMVSHNSTQWKCFSDVLAEIESRGIYIPIRHINNSGAILNYPEY
                     KLNMVRPGIMAYGIYPSPENESKAGLLPVMSFKTGIVLLKDFPAGHGIGYNSTFVTSR
                     PTRIATIPVGYGDGYPFILSNQSTALIHGQRAPIVGRISMDMCTVDVTHISNCKIGDE
                     VVLLGQQEKECVSANEIAAKAQTISYEILCALGKRAPRVFLQKGKHKTVEPRLKRIFV
                     PEEERSLSRIDNIIRHCLLARARNEELGNAIYYEMFETLFGNRDRRLELRSGFRYDIR
                     IAKMPKTKDVVLSKDYLHVGMHVQYKKTLRDNIFMIGCASSHSQLNALFKDPRCEYRW
                     LLNDGGNLSGKRDFTVERVRIDNEDVPIIETKSTKRGYEVWCGSDRLKKKINQDVKLE
                     MDISTKQARQKRTFPVYLVYPVRGLEIKFDYANAGIKNVREESFFAGKHPQPSVTGKK
                     GKSIAVKISDEEWIFPTSGVIFIWDD"
     gene            complement(18310..18732)
                     /locus_tag="PLR13_05735"
     CDS             complement(18310..18732)
                     /locus_tag="PLR13_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HQC18871.1"
                     /translation="MIRKILCFVVIGIFLGVTAVVADSSDREKAAIASAEKWLKLVDQ
                     GKYIESWKESADYFKRAVSQEQWEQAMKSGREPLGKLIFRKVTNALYTTSLPGAPDGQ
                     YVVIQFNTSFENKKSGIETVTPMVDKDGRWRVSGYYIK"
     gene            complement(18807..18998)
                     /locus_tag="PLR13_05740"
     CDS             complement(18807..18998)
                     /locus_tag="PLR13_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18872.1"
                     /translation="MKRPASLVVGILLFVMAVAQAGRFIIGLNVTAEGVEIPVWISAV
                     AAVALGALGIWLLKERTRQ"
     gene            complement(19060..19512)
                     /locus_tag="PLR13_05745"
     CDS             complement(19060..19512)
                     /locus_tag="PLR13_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HQC18873.1"
                     /translation="MVLDVIENLHKYSALNQGFARAIDFLLRPDLRNLALGKYEIDAE
                     RVYAMVSKDSGCKKEEALLERHEKYIDIQLVLSGTDTMGWKPKSLCKQPTGAYDEGED
                     IQYFADEPDSWIATRSSSFVIFFPEDAHMPLISSGQLHKVVVKITTSW"
     gene            complement(19516..20655)
                     /locus_tag="PLR13_05750"
     CDS             complement(19516..20655)
                     /locus_tag="PLR13_05750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQC18874.1"
                     /translation="MAGKTGLKETAKIRIGVLYGGRSGEHDVSLCSAASVVAALDRKK
                     YKVTAIGIDRDGRWYVQEKPEIIPDKDFGKKMALKKKGMWLVNHFEQRNKLHLYDIKN
                     KNKEVVVDVVFPVIHGTFCEDGTLQGLLELAMAPYVGADVTGSAVGMDKDIAKRLLCE
                     AGIPVVPSVTVNKQDWKENNRVIMKQALSGLGLPLFVKPVCAGSSVGVRKVKKEEELA
                     KAMNFAFQFDTRVMIERAIDCREIECAVLGNENPAGSVLGEIIPNHEFYSYEAKYIDP
                     DGAALKIPAEINKTLSDKIRKIAVEGYMALGCTSMARVDFFLDKKTNKFYLNEINTLP
                     GFTSISMYPKLWEATGLKYSILLDKLITLAIDRHTKKLEIKTEGI"
     gene            20859..21395
                     /locus_tag="PLR13_05755"
     CDS             20859..21395
                     /locus_tag="PLR13_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18875.1"
                     /translation="MPLYFNAAQMPVTKKAFNDAENLVSRQFRLSDADFRKNRYEVKT
                     LAYLDEQEIKDGTFAHLCKYTYERPDGLANRSEKVFNFYRICLQDNIILDAVERANPF
                     IKLSPLMLYIAVHELIHILRFASGEIKFDADAKEKEKEEVIVNNLTRAALLPVKGYDL
                     EIVLDCFSSHFKNFDSFN"
     gene            21414..21686
                     /locus_tag="PLR13_05760"
     CDS             21414..21686
                     /locus_tag="PLR13_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQC18876.1"
                     /translation="MPIYEYKCQKCGKQFEAFQGITDPELKTCKFCKGKVHKMMSLSS
                     FSLKGSGWYVTDYKGKKPGAAPAKGKTETTDSAAKTETSKTESSES"
     gene            complement(21866..22966)
                     /locus_tag="PLR13_05765"
     CDS             complement(21866..22966)
                     /locus_tag="PLR13_05765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQC18877.1"
                     /translation="MLGIFPQDDHNQTLRLKRFFMALGSYLMWFAICVMFYAQGQMTR
                     GNIQVLVISSLGILFCNVLIYAMIRSGYNKRFKDPSLTLLQMIIATFWVMVIAYYADT
                     TRPLVLLTYLVVFIFGLFRLSVWQFLFLSAFAVVNYSLVIFLLYNINPDLVSTSDVLS
                     IVVLALVLPWFSLVGGYITRLRARISHAFSTIEKLAIIDDLTQVFNRRQMFKILEYQK
                     ALVDRGIHPFSICIFDLDHFKRVNDTYGHVAGDFVLKTVAQETQKNLRNIDHIARYGG
                     EEFILILTNVEKSQAILCADRIRNLVKKIVFENMPDDFKMTISVGVTEYQAGEDIQQA
                     INRADTALYRAKTNGRDRVEYEPMSKDKGEKG"
     gene            complement(23104..24147)
                     /locus_tag="PLR13_05770"
     CDS             complement(23104..24147)
                     /locus_tag="PLR13_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18878.1"
                     /translation="MRDLGFKKEITTGYYLDPFNGFQEKKITIESRYDEITGVASRIL
                     PYRMRIAQKPDMNEYLEKSPESLCPFCSDLFDKTTPRFIPSVMEDGNFKRGEACLFPN
                     VFPYDQNNNVAIFSSRHLVAMNELTPEFMRDGFLVCRDYFHRVVELNLGYKYCSINWN
                     YMPPAGGGLIHPHLQTIAGYRPTSFMQKLLSSAQNYAANSNGENLWSNLIALEKKADK
                     RFIADTGVVCWLTSFAPKGMAGEIDFIFREKTSFFDLTESNFSEFLAGLSRVFSYLYT
                     NNFISFNLSLYATMNNNNYFCVQGKIVPRFVIYPLGTSDLNYFEKLHNEIICPTVPEE
                     LCRELQPYFKSLL"
     gene            complement(24190..25221)
                     /locus_tag="PLR13_05775"
     CDS             complement(24190..25221)
                     /locus_tag="PLR13_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18879.1"
                     /translation="MKVNAIPIKKETELNFHKLMDMVQPDDSLLLLMYGSPDPDAIAS
                     AMALREILQRTKGLSKSAFVSTEPLDRQQNIELVSCLHLDIQRMNQVDVASYRVTALL
                     DAQPSFFTHFPDALRPQIVFDHHPYEGQWSAELMDVRPNYGALSSILTEYLLCSRIKI
                     PRNLHTALLYGIKTDTDNFDRATIIEDISAYAYHTKYANMPLIRRIELNQTPDRFLKY
                     FHYAYHHMNNFRGRRICFLGNVESADVCVQIADFYLHLIGTYYVVVTGIVQDKLIIIF
                     RGDGYRQNCGAIAQKAFGAVGKGGGHRSAARMEIHLSALKEILGGELSQKNVERFLFS
                     SLRREQKTT"
     gene            complement(25255..26307)
                     /gene="selD"
                     /locus_tag="PLR13_05780"
     CDS             complement(25255..26307)
                     /gene="selD"
                     /locus_tag="PLR13_05780"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(26254..26256),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQC18880.1"
                     /translation="MEEKKGVRLTETVQSAGUACKIGPADLSEIMRDLPLIHDPNLLS
                     GFEHAEDAGVYKISADMALVQTVDFFTPTVDDPFTFGQVAAVNALNDIYAMGAKPLTV
                     MNIVCFPIKTMAVSVLRNVLQGGLNKIHEAGALLIGGHSVEDNEIKYGLSVTGVAHPD
                     SILYNRGARAGDKIILTKALGTGIISTAIKAGEASPKQIQQVTVTMTTLNKKSAQLMI
                     AAGDIHACTDITGFGLLGHACEMIEGSDVGLRIHSAAIPALDGVRDLMETGFVPGGLY
                     RNKDFRIKQVVKEKTCPDWLFDLLFDPQTAGGLFFSVPGDKAQNLAEKMRGAGIADAA
                     VVGEVVPNHPGTILVY"
     gene            complement(26435..27655)
                     /locus_tag="PLR13_05785"
     CDS             complement(26435..27655)
                     /locus_tag="PLR13_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase"
                     /protein_id="HQC18881.1"
                     /translation="MSDKKLYIVLMGLPARGKSTLAIRLRDYFLKEGIPTRIFNNGNL
                     RRLYLPHLETSSADFYSPENETAVALRKKFARMNMDRARKYLFNNGMIAILDATNAGR
                     ERRGLIEKTLNDHDLLFIECVNDDDDILKMSIQEKIRLPEFAYLKDKATREFLKRIDY
                     YKAIYMPLKAERNYIRIDTLQKMIVEEKHSDVIPYYERLRDYLVTDEVKNLFLIRHTE
                     TEYNVENRIGGDPALTPKGLKQASALGRFFRKKKISYIFTSPKQRTIHTAKLIRSQQD
                     DCRIIPLKEFDEINAGICERMTYEDIEQKMPHVFKKRGADKYNYIYPEGESYALMKPR
                     IMEGIKKAFFLNRHANSIMIVGHQAVNRMILSYFLYRREDDVPYIYIPQDRFYHIVSK
                     QDKKIFELKRYDSD"
     gene            27832..29217
                     /gene="thrC"
                     /locus_tag="PLR13_05790"
     CDS             27832..29217
                     /gene="thrC"
                     /locus_tag="PLR13_05790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQC18882.1"
                     /translation="MPIRYYSTNRHLKNVAGITPFTGKVSFREALLKGQASDEGLFMP
                     DTIPQLAITDIQQLNGKPYRDTALLVAKAFLADEFPPDILQQVVTESYNYPVPLEEVR
                     PDHFVMRLDRGPTASFKDFAARMMARLMSHCRPKGERLNILVATSGDTGSAVGEAFKG
                     VTGIDVTILYPENEVSGMQKKQLDTIGQNVQALSVAGKFDDCQNLVKQAFSDPRNEKF
                     NLTSANSINFGRILPQIIYYVYAYAQLAQNKEEAIFSVPSGNFGDALGCEYARRMGLP
                     VAKLVMPTNENDEFPIFMATGKYNKVSPSRACISNAMNVGHPSNLARFFDLYGGTVDR
                     VGQVHKYPDLEEMRRHIFSVSVSDSQTRETIKNVYKRYQAVLEQHGAVGWAGLEEYLK
                     TTPEQKLAICLETAHPAKFPEEIEKLLGLTVPVPESMQNIDLREGSAVAMSGDYSSFR
                     NYLQKTLKIKE"
     gene            29622..30104
                     /locus_tag="PLR13_05795"
     CDS             29622..30104
                     /locus_tag="PLR13_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQC18883.1"
                     /translation="MLKKIVMAILFIMISAGSSMAGGAVEKDYGKKPWMVDIEDLTVK
                     NENFRTTKWTGQNLQMTVMSLKPGEEIGLEKHDKGDQFIRVEKGNARVVMGVSKDKMT
                     FDEKVSDDWAIFIPGGFWHNIINEGDKPLKVYVIYAPPEHPARTVHKTFKESAAAHSH
                     "
     gene            complement(30559..31062)
                     /gene="mobB"
                     /locus_tag="PLR13_05800"
     CDS             complement(30559..31062)
                     /gene="mobB"
                     /locus_tag="PLR13_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HQC18884.1"
                     /translation="MTVSLISIVGKSNSGKTTLVEKLIPELIKRGYRVATIKHNKHGF
                     DIDHEGKDSWRHKRAGACATVIACPTRVALIEDLERDQTLGEIRDKYIKGADVILAEG
                     YKGNPFPKIEVFRSELKRELLCGKEDNLLAIASDTKLDTGVPCFDINDIQSIADLIEE
                     KFLKSRV"
     gene            complement(31083..>31468)
                     /locus_tag="PLR13_05805"
     CDS             complement(31083..>31468)
                     /locus_tag="PLR13_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC18885.1"
                     /translation="ASIVTISISNTRATAYGYMGPIKAALDATVAFLAKSLSEMSKAR
                     VNAVCAGPLKTSASAGIPGYVNNYLFAEQLTLRKAALKTEEVASTVLFLLSPASGGIN
                     ATGVVVDAGMSCNYFDQDVVNKAMR"
BASE COUNT         8451 a         7685 c         7279 g         8053 t
ORIGIN      
        1 aatgtaaaaa ttacggccgc aaaggctata atattaaact atttcaacag cataagccca
       61 gcccgcaacc ggaataaccc aatcgcgagt cgttggagta taagaagtcc cccatcgtat
      121 gtcaaggata taatacgcgt tgtccagcca atttagaaat gtcctatttt taccaaaata
      181 gaaatgtcct attagaagaa aaaatataga tatcaatatc gttctcataa aacagttggc
      241 gttgtccccc aggttttcca cctttgcttc catggatgat cttttgatgg tttagggggc
      301 cggttatatg ctctcaggtc tttcttaaca gccaccatct gtttgggtct ttcggttatt
      361 tctttgtatt ttaaagctct gcccttgctg atgatgagaa gagaaccatc tcttctcttt
      421 tcaatatgga cctttttact atcgttctcc tctaattgat aaagctttct gttgtaggca
      481 atggtgtgat cattacggat cgtcctttgg tctttgatac agagatattt gtcccagtta
      541 aaatgacgag gaggtctgac atgaacatcc gtctcattgg ccggacacac tctgaatttt
      601 tcattatatt ggggcaagta cgtttccaga aacccattgg cttcatcttt tgtcttgatg
      661 ccgtggagcc tcatctcctt gaccagccga tcctgaagga ctccaaagag ccgctccacc
      721 cttcctttcg cctgcggaga caaagcatga atgacttcca cgccaagctc tttaaggacc
      781 ctctcaaact gactgagaaa ctttagatca cttgcttcat cccattccgt aggtttcctg
      841 gaagacttgt aggtcgtgtg acgatcaaga taaacgctca tgggaagacc gtacttcttg
      901 acataaccct tgaagctgtc catggccggc aaggtccctt cataatcata aaattttgca
      961 tagattgtat tcgtggcatc atcgatatag cccattaata ccagcttggg acctcgacct
     1021 tccaaccagt catgatggga cccgtccatt tgaaccattt ctccaaaaca ggatcgcctt
     1081 ggacgccatt gcctgtatgc tgatcgcttg cgatgcttct tccacaaacc ggcccctatc
     1141 agccaattcc gcagtgtctc atcgcttaat cggataccat ctcgctccca tagcttctcc
     1201 gtcgcaaaag tgggtccaaa atcagaatac ttctccttgt atagagcgat gacgttctct
     1261 tttgtcccag cggataatcc gcggtttgat ggccgaccac gggatctatt tataataccc
     1321 ttatcgcctt cctcgcgaac ggccctcact aaccgcctga tctgacgctc actgagtctt
     1381 aatatgccgg atacttgctt ctgactgatt tgtctgtcta tcgccgactg gatcaacttc
     1441 agacgcttta attctcctaa actcatgacc accatgtccc tttcagccat aattacctcc
     1501 ccccttgcag gaaacaatta cggctttatc ttaggacatt tctactttgg tggaatagga
     1561 cattatcact ttggcggaac atgatataat acgcatccga atggccttga aatttatcta
     1621 acaaagtatt aacttaatga aaattgtcct gtgaattttt caaaacaaaa tttgttcatg
     1681 cccgaggagg tctgaaatgg gattgaaaac cgctgatcaa tacgaagaaa gcctgcgtaa
     1741 aatgaatctg aaagtctacc ttttgggtga acaggtgaaa tgccccgccg atcatccaat
     1801 catcaggcct tccatgaatt ccgtaaaaat gacgtacgcc ctggctcaaa gccccgagca
     1861 tgaagatctg atgacggcga aatctcatct gaccggagaa aagataaacc gtttctgcca
     1921 tctgcatcaa agcacggaag acctgatcaa aaaagtaaaa atgcagcggc tgctgggaca
     1981 aaaaaccggc gcctgtttcc agcgctgtgt cggcatggat gccatcaatg ccgtggacag
     2041 cgtcacgttc gaaatggata agaaactcgg cacgcaatac catgatcgat ttatcaggtt
     2101 tcttaaaatg gtgcaggaag aagatcttgt tgtcgacggc gccatgaccg atccgaaagg
     2161 cgaccgcagt ctgccgccca gccgtcagac cgatcctgat ttgtacaccc atgttgtgga
     2221 aaaaagaaaa gacggcatta tcgtgcgcgg cgccaaggcc caccagaccg gagccgtcaa
     2281 ctctcactgg attctggtga tgccgaccat cgccatgacc aaagacgacg ccgattacgc
     2341 gctttccttt gtcgcgcccg cagacgcgga cggcatttcc tacatctacg gccggcaatc
     2401 ctgcgacacg cgcaaactcg aaggctgtga aatagatgtc ggcaaccggc aattcggcgg
     2461 tcatgaagcc ttgatggttt ttgacaattt attcgttccc tgggaaaatg tcttcatgtg
     2521 cggggaatat gaattcagcg gactcctggt ggagcgcttt gccggctacc atcgtcagag
     2581 ctacggcggc tgcaaggtcg gcgtgggcga cgtgctgatc ggcgcggcgg cactggcggc
     2641 cgattacaac ggcgcggcca aagcctctca cgtcaaagac aagctcattg aaatgacaca
     2701 tttgaacgaa acccttttct cctgcggcat cgcctgctct gcgcaggggc acaggaccgc
     2761 ttccggaacg tacctgatcg atctgctgct ggccaatgtc tgcaagcaaa acgtgaccag
     2821 atttccctac gaaatcgccc gactcgcgga agatatcacc ggaggattga tggtgaccat
     2881 ggcctcggaa aaggatctgc gtcatccgga aatcggcaaa atagtcgaaa aatatttcaa
     2941 aggaatcgct tccgtgccga ctgaagaccg atgccgaatt atgcggctgg tggaaaatat
     3001 cacactgggc actgccgccg tcggctaccg aacagaatcc atgcacggcg ccggttctcc
     3061 gcaggctcag cgcatcatga ttgcccgcca gggcaatctg gaacacaaaa agaaactggc
     3121 caaagaaata gccggcatca aggattgacc gggcttgcct tgatgaactg caaaatatca
     3181 gaaagaaagt tgattggatt taaatactgc ggcgggtgca atcccgtcat caatagagca
     3241 caactcgttc aggacatcca aagacggctg tcggctgagt tcactcttgc aacggatcaa
     3301 tctccaactc aatgggacat cggtattctc gtttgtggct gtctgagcgc ctgcgcggat
     3361 aagccggatt tcaggaatct tgcccgcaga tggattatca ttgccggcaa ttcggttgat
     3421 tacggcgatg cgccggaaaa ggatttggct gaaattgttc taaacaaatt gaagctataa
     3481 aatattctaa ttcggatatc cttgaggatg gttgagtcaa aaagactcaa cagaacaatg
     3541 cgtaaaatca ggccttaaat ctctcaaagc cgttcatctc cttgtgaagc cttcatcata
     3601 cattcgtgga attatctaat tcccttaagc attattccga tccttcccgt ttttatcaat
     3661 ggatatcatc gtgacatact atgatatttc tattttattt catagccacg ctctgaaata
     3721 aaataattct tgacagtgcg actatgaata tgtatcttaa aatcactctc gcgggaggaa
     3781 atgatcatga cagacaagaa cagacccaag acccgaaaag accgcattat ggatgccgct
     3841 ctgcgcatct ttgccgagaa gggttttcag aacgccacca tcaccgaaat cagcaaagcc
     3901 gccggtgttt cggaagcgac aatctacgaa tacttcggca ctaaggaaga ccttctcttt
     3961 gcgattcctg aaaaaataac caacgacaca tttgaggaat cttcaaaggt cattccctac
     4021 atcaaaggcg tggagggcaa aatcagggcg attttacttt tctatgtcaa gctttatcaa
     4081 tccaatccca actattccgc actggttctc ctgcaactga tgtccaacag gcgctttcga
     4141 cagacacccg cccatgccgc aatacgcaca tcggcgcata ccctgctgga ctgcatccgg
     4201 gaaggaatcg ccgacggcac attcaaaaag gattccaatc cctacctgat tcgctccgtg
     4261 tttatgggta cgatcgagca tttattcatt cactggcaca tgcagggcat gcccaaaaaa
     4321 aaaccgggca tcgcggagat gcttgacgcc ctgctcgaaa ccgtttttga cggcatccgc
     4381 gcgaggaaag aggattccgg aacaacgctt tacctgaaac tggacgatgc cggcctcctg
     4441 gaaaaaattc tgaagaaggg cagagaacgt catcaaaata aaaaataaga taacattttt
     4501 agcaaacaaa gaagaggtac tgccatgaga acgagagaac aatacattga aaggctccgg
     4561 aaaatgagaa ggaacctcta ccatgacggc gaaaagatcg accgtgatga tgagatccag
     4621 gctcccgtgc tcaatgtgat gggactgact ttcgacgccg ttcatgatcc ggagttaaaa
     4681 gatctgtgca ccgcgacttc ccatttaacg ggagaaacga tcaatcgctt ttgccatgtc
     4741 catcaaagca cagacgatct tcacaaaaag caggacatga cccgggcttt atgccggaag
     4801 gcagggatgt gtattcaacg atgcatgggg accgatgccc tgaatgccgt caacgccgct
     4861 tcgtttgagg ccgataaggt caacaacggc aatacacaat accacaagaa tttccttaaa
     4921 tggctcgaac gttttcagaa ggaagacctt gtgggaagct gcgcccagac cgacatgaag
     4981 ggcgagcgcc tgaaacgacc cgcggaacag acggacccgg atatgtacgt ccacgtcgtc
     5041 gagaagaaaa aagacggcat tgtcgtccgg ggctgcaaac tccacatctc cgaggcgtca
     5101 atctccgatg aaatcattgt ggtgcccacc cgcgccctgg gtcccgatga aaaagattac
     5161 gccgtggctt ttgccgttcc ctccgattac gaaggggtca agcaggtcgt ttccattcac
     5221 aaccagcaca agcgcaaaca tatcaaacgc ggcatagaca tcggttattc cgattcctac
     5281 atcattttcg aggactgttt tgttccctgg gagcgggtct tcctctgcgg cgaaaacgat
     5341 cacggcggaa tgtgcgccct gctgttcgcg ctgttccacc gccacagcta ttcgggatgc
     5401 aagcccgcca ttggggatgt ttttctcggc ctggcagctt tggcggcgga atacaacggc
     5461 atcgagaaaa cgcctcacgt gaaagagatg ctggcggaga tcatcaaagt gtccgagctg
     5521 ggttatgccg cagggtatac ggcttccagc atgggcaagc ccgaagtcat tatccccggc
     5581 ctgggtatgt gcccttacgg accaggcagc tacatcccca attccattta ctgcaacgtg
     5641 ggccggtgcc taacgggaga agcggttttc catgaacagg aactgatctg ccggatcgcc
     5701 ggcggcctgc cgtccacctt cccctatgaa ggcgacctca cgcatcccga cctgaagaaa
     5761 gttctggaaa agtatctcaa ccggaatccc aaggtccctg tggaagacca gatcaagttt
     5821 tggatgacgc tgggcgacta taccatcggc accatggccg gtgtcatgaa ctacgggaac
     5881 tatcacggcg gcggatcgcc catcatggag cagattgcca ttacgtccca gtatgatatc
     5941 aaggacagaa agaaactcat taaacgtctg gcgggcatcg gcggttgagc cagagaagtt
     6001 tgataatttc actggaaaca gaaacaatat tgtaatagac atcatttgcc ccgtcgccaa
     6061 caacagagga ggcgttagat ttctcaagga gaataaaata tgctcgacgt atcccaattt
     6121 tcattaaaag ataaagtggc ggtgattacc ggcggaggcc gcggcatcgg tcaggctatc
     6181 gcgttcgctt ttgccagggc cggagccaag gtggttgtga ccagccgcaa ggcgccggat
     6241 ctggaagcga cagccaacga aatcaaagct ttcggcgggg acgcttaccc gctgccggct
     6301 catctgggca aatcggaaga aataaaaaag atgattgacg ccgtcatggc caaatacagc
     6361 cggattgata ttctggtcaa caacgccggc gccagtcccg cgaaaggttc cgtcctggag
     6421 tgcgacgaac gcctctggga caagctgatg gatatcaacc tcaaaggcgc gtattttatc
     6481 agccaggcag cggccaatat catggtcaag cagggcggcg gcaagatcat caatatcgcc
     6541 tccatcgacg gtcacaatcc ggaaccgggt ctgagcatct acgctatctc caaagccggc
     6601 attcgaatgc tgaccaaggc ttttgccagc gaacttgtcc ggtacaacat tcaggtcaac
     6661 accatcgctc cgggacccat cgccaccaag atgttgaaat ccaactggga atttcttccc
     6721 cctgaagacg ctcaaaaagt gaaagaactg gtggaaaaag cgctgccctc cggccgcatc
     6781 ggcgatcccg atgaaatagc cggcgccgcg ctttacctgg catccgatgc ctccagctac
     6841 actaccggag cggaaattgt aattgacggc ggtgttctgc tcggcatgca cccgtcctga
     6901 caggagaaag ctcgatgatc agtcgaacgg cagcggttct ggtaatcatt gatattcagg
     6961 gcaatctggc tcaggccatg tttggcaagg aaaatatttt tgccaatgcc gtcaagttga
     7021 ttaaagcatt caagaccttg aatctcccga taatcgtcac ggagcaaatt ccggggaaac
     7081 tgggtaaaac acttccgcaa atagcggacg aaattgaaga cttcaatccg atcgcaaagg
     7141 aatccttcag ctgctggggt gaaaaaaatt ttaaagaacg actggaaagc ttaagccgca
     7201 ggcacgtcgt gctgctgggc attgaaacgc atgtttgcgt ttatcagacg gcgctggatc
     7261 tcctgtccga aggatacaac gtgcatctgg tggccgatgc cgtttcatcg cgcacgccgg
     7321 agaaccgtca gatcgggatt aaagcgatga aaagcgccgg agctcatatc gccagcacgg
     7381 agatggtctt atttgaactg ctgcgcaccg ccgccgatcc gaaggccaaa gaattcttca
     7441 agatcgtaaa gtaagaagaa attcattatt cagctcacaa accttctttg aatttactgt
     7501 cttctgaaat attattttct gattccgccg aaaatcacct tcagcccttt ttatgattcc
     7561 gctgtggaac atcacaattc tatctgggtg ccgagttcaa ccacccgatt ggcgggaatt
     7621 ccgaaataag acgtcgggtt ccccgcattt ctggacataa aggcaaataa tgatttgcgc
     7681 catttcatca ttttcgattt tcctcccgta agcagagtct ccttgcccag gaaaaatgtc
     7741 gtcgtcatcg ggtctgtaac aagcccatac tgggcaagta ttttcatcaa ctgcggcaca
     7801 ttgggtttct gcatgaatcc gtaaaatgcc tccacccggt aaaacccctg acccagatcg
     7861 gctagcttaa tgcgttcacc ggcaggaacg gccggtgtat ctttggtgat aatggaaaga
     7921 agcactactg tttcatgcag gatgtgatta tgttttacat tatgaagcag cgtcggaggc
     7981 gttcccacgg gagacagcgt cataaaaaca gcggttcccg gcactctggg catatggcgc
     8041 ttcggcaatt cagaaagaaa actttccagc gggaaccttg ctccgaccaa attacggtaa
     8101 agttcattcc gtcctttttt ccaggtggtc atcgctaccg caatgattgc tgcgatcaag
     8161 agaggcaacc accctccatc agcgagcttg aaaaagtttc ccgtgaaaaa agcgatatcg
     8221 actaaaagaa acagtccgac gaggggaatg actttccgca ggggccatcc tctgtgatgg
     8281 gtcagcacca gaaaataaag aattgaggtg atgatcatag tgcccgtcac agcgatgccg
     8341 tatgcgccgg ccagagcgcc ggattcccgg aaccccgtca ccaccgcaat acacgccatc
     8401 atcagggcat agttgacaga ggggatatat acctgtccct gcatatcgct ggaagtgtga
     8461 ataatccgca cccggggaca taatcccagt tgcgcggcct gttgcgtaag tgaaaacgcg
     8521 ccggatatta acgcctggga agcgatgacc gtggcggccg tggaaagtcc gatcatcggg
     8581 tagagcagaa tttccggcac tattttataa aaagggttga cattcatttc gggatgcgcg
     8641 agtagcagag ctccctgccc gaaatagttg caaagcaatg aaggaaaaac aagtgaaaac
     8701 cacgacaggc gaatggcgtt tcggccaaaa tgccccagat cagcgtaaag cgcctcgcaa
     8761 ccggtaatac aaagcaccac cgatcccagc accacaacgc cgtgcattcc gttgttcaga
     8821 aaaaactcca cggcatatag cggattcaac gcccgaagga cggaaggttc gcgtacgatc
     8881 tgcatgatgc cgaaaatacc aatggacaga aaccataaaa ccatgatcgg agcaaacacc
     8941 tttccaatat tcgacgtccc tttgcgttgt aatacaaaaa gcagaatgag cacgacacag
     9001 gtcaacggca atataaaagg cgcagcagca tgggtagcaa cctccaatcc ttcaattgcg
     9061 gaaagaaccg atatggccgg cgtaatgacg ccatccccgt acaaaagccc ggcgcccaga
     9121 atcccggcaa gcacaagaac ggactgaaca aggggagaaa aacttttctt cgacacattc
     9181 aaaagactga ccagcgcaaa aatgccgccc tcgccgcgat tatcggcacg aagaaggaaa
     9241 atgacatact tgatgctgac gattacgaac atcgaccaga agataagcga caaaacgccc
     9301 agaatattcg ttatgtcggg agcaagggcg tgctgtccgt aaaaacaggc tttgatggcg
     9361 taaagagggc ttgtgccgat atcgccgaaa acaacaccga tggatgcgaa ggcaagaaaa
     9421 atgatttttt tattaaaccc gccggaaggg ggctctcccg gtgattccgt ggccgattca
     9481 ttctcggaat gtgctgaaaa cataaaaaac ctccttgaat ttcccgggga gaataatcat
     9541 cccggacgaa tccggtcggt tctcctcatc gaagcctgcg aagttagctg aagggctcgg
     9601 gccgaaaagg ttaccctacg cgcaagcgat acgcccctga aaattgggtc ctccgctcct
     9661 gaaaatcagg actaggcata tgtttggcgc taattaaatc atcttttcag gcgtgtcaat
     9721 acgtttgttg gagttcattt ccgaatggtt ctattacatt ttgacaatta aagactccgg
     9781 ccttgacttt tgcaattcgg tgttgtaaat aaaatgcgac cttttaaggg aggcagttta
     9841 tggtcccggt cgccaccaac ccgtatgtaa atagcgtttc tttcattgat attcttgtgc
     9901 agggcaaccc tgcttttttt ttatttatcc aaaaaaaatt aaaaaacatt ttaattcccg
     9961 tgacaacggg gaagaaggag gatttttatt atggcacaag ctcaggtatt tgaaaataaa
    10021 cagccggttc tgggaatcaa cagtcttggg cgaatcggga aactcactct gtggcatcac
    10081 atcggaagaa agtattttaa ggaaatcatc gtcaatcagg gacgggaagt aggcacgggc
    10141 cttgattccg tggcccaagc gatcgagaaa gattccacct acgggttgat gcacaagttt
    10201 ttatacggcg tcaaagcaga acgcatcatt cagattgtcg atgaaaaaga ggggaaactg
    10261 ctgatcgacg gcattcccgt gaccatcctg cgcgaggcaa gaaatcccgc agatgttccc
    10321 tggaagaagt acggcgtcga tgtcgttgtg gactgcacgg ggaaattcaa ggatccgacg
    10381 gtcgcggcag atcacaagag cggttccatt cgtggacatt taaacggcgg cgcgaaggtc
    10441 gtgatcaatt catcgccttt caaaatcaag aacaaggcgc tggcgcttcc ggatgatgcg
    10501 gtgacaatga tctacggcat caatcatacg tcgttcaatc ccaagaaaca tctgatcgtt
    10561 tccgccgcat cctgcacgac gacgggattg gctcacatgg tcaagccgtt gctggagaat
    10621 gaagagacca gcaatattct gacggcctcc atgtccacgg ttcacgccgt taccaatacc
    10681 cagagcgtgc tggacagcgt tcccaaggcc ggcgaaaaag atttgagaaa aaaccggagt
    10741 attctggaca gcatcattct gacatccacc aatgccgcca aagcgctggc ggaagtcatt
    10801 ccgcaggtcc agaatatcgg cttcatggcc gattccatca ggataccggc cagcacgatg
    10861 tcgctgattg tcctgaatgc aacattccag gcccgcagcc agggagcggc tacggccatc
    10921 aatacaaaaa tgctcaacga gctatacggc aaagcggccg ccaacaaccc tgaaaaactg
    10981 atccggttta cgatggaaca gaatgtgtcg gctgatcttt tcggtatcga cgcggccgtc
    11041 atcattgaag gccagtttaa tcacacgcgc acggccttta ttaatctgga tctggcccat
    11101 attcccaata tgccggcaga ggttgccaat tccttccccg acaaggtcat gaaagttccg
    11161 gttgtgcatg ccaagatatt cggctggtat gataatgaat acggcagcta cacgaaccga
    11221 atgggggatc tgacggttca catccacaaa aatattttat aagaaagttt tagccgtttt
    11281 tttgaggtat ctgcattcac cgaccgctcg cttccgcggt cggtgaattt ttttctgcta
    11341 ttattcggtt tcgctttcgt gccggccaag gtaaactgtc ccgctccaac cgtcgatact
    11401 gataaaatca cctgctctga tggtctgatt ttctatggcg gcatattcat ctgtttcgta
    11461 aactttcagt ttactgaatc cgacgacacc gaccttgttg agttgcggaa tcgtgaccgc
    11521 ggcatgcgat gtgctgccgc ctttcgcggt cagcaggcct tccacttgca gcaaaacacc
    11581 cacgtcatcc ggaaccgtat cgggacgcac cagaatcaga ggcgtttgcg gttcttcact
    11641 gcggaatcgt ttgatatccg cttccgaata aaccgcccga ccggccagag ctccgccgtt
    11701 gacgccgatg cccgctccga tcatggatga ctgcaacgcc ggagaatctt taaaacgtct
    11761 ccagttctgc gtggtaatct gattcatgtc gcgggtttgc agaatgtaca atttatcgcg
    11821 cgtcggccct tcaaaggtaa attcgatctc ctggtgattg aacccttttt cgtaaatgag
    11881 gatctccgaa attctcagca gttccgcata aatttcaggg aatttatctt ccagggaaat
    11941 cttggaatcc cggcattccg cgatgcgctg cttttccgaa acgggatacg tttccaccag
    12001 tcccgaaacg atatcgtccc cctgaacccc gaaaatgaaa tctccgtaga gggtcacatc
    12061 ggaagacgtg cttttcggtt ctcgggtaaa aataacgccg gatccggaat tatcgttgag
    12121 attaccgaaa accattttct gcacgatgac cgccgttccc catttatcgg aaatatgcat
    12181 ttgccggcgg taaatttccg cctgttccga ataccaggaa tcgaaaacct gcaaaatggc
    12241 gtcgcgcagc tgatcgagcg gcttctcctt aaattcgatg ccgttgtcgg tcagcccttt
    12301 tttataggac agggcgatct gtttcatctg ctcgggtgaa aactggattt ttctttccac
    12361 gccgtacttc tgtttgaatt cgtcaatgat cgcgtcgaaa aaattacgat tcagcccttt
    12421 aaacatgccc cacatctgta aaaaccggcg ataggaatcc caggcggccc atttaaaacc
    12481 atctttctcg ctgagatttt ccgcaatcgt ctcgttgatg cccacgttca aaaaagattt
    12541 catcatgccg ggcagggaaa ccgtggcgcc gcttctgatc gagagcagca gaggattgcg
    12601 gcggtcgcca agcttccgtc ccgtaatctt ctccagtttt tttatttctt tgttgacccg
    12661 aacagccaga tcgttaaaaa tatatttata cccgtaaacc gcttcatagc ccctgaacac
    12721 ttccgtggtg atgatgaaac cgggcggaac attaaacccg aatgagacaa gctctttgag
    12781 gaaatatccc ttgttcccga gcagaatctg attatctact tttttgttct ttttgtagaa
    12841 agaagacacg gccagttcgg gattgtacgc cataacgacc ttcagaattt gtttgttgtc
    12901 cctgaacttt tcggattccg cattcagtgt tttaataact gaattaacga aattatccag
    12961 cacctgaatg ccgaaagcgg atgcgatgat gccccggatg aaattttccg actgtgcata
    13021 gatcagtttc tcatgttgct tttgtccgga ttttctctcg gaatcgattt cccgccggta
    13081 tacctggccg atcacaacag gcaggcaggt ggtgtggata tcaatgtaat agttgcgaat
    13141 aatgtcctga atatccttgg aaatgaaacg aaaaatatcc acgtactgct ccagggaaaa
    13201 ctgcttgatg gacaaagcgt tggtgacgta tttgattttg gctgccagac cttccgtggc
    13261 gatgccttca atttccagcg cattcatgta aagccaaagg tatgtgtgaa tttttctgat
    13321 cgtgtgcttg gtaatgaact tgagattgaa ggatttgctc agttcctcga acagcatggt
    13381 ggccagtgtt tccagccgca gagaaaggcc gaccgcttcg aattttcttt cgtaatatgt
    13441 tccgtacatg gagggaatgc cggcggcaat atgcctcttg taatagatgt tctcataaga
    13501 aggcgtcttt ttgggcgaga gaattctttc cttgagaatg gatagaaact tcagaataat
    13561 cgtaatgctg cgatgatagt tcttcgtcgt caaggagcgt ttcaacgacg agatatccgc
    13621 tttgggaaaa gcgttgaccg cttccagatc cttgagcaga tcgataaact gtgggtaata
    13681 ttttttgtag atcagctggt agatccggat catgattttg acccgttcct gatccctcgc
    13741 attgatgcct ttgacgttcg ccagttcctt acagactttt tcgttatccc agtccagaaa
    13801 tttcaggggg ctttggactt ttgtgaagaa atatttaaag gctttgtgca ttccggtgaa
    13861 atattctccg ggattcatca cctgttcgta aacttcaacg ggaagatgag agcgaagata
    13921 actcatatct ccggtattcc agtactgaaa aatatcctcg gtaaatttaa cgagcaggct
    13981 gttgctttcc acgtgggatt gtttccgaaa gaaatacacc agcttgtcgt tgcgcatgga
    14041 cagttcgtcc acttttgtcg tgatgtcccg gagctcacct tccgcaccga tttcactgaa
    14101 ataaacggga aagatccgga gaagctgttt gatcagactg tacgcgggag cgatatccgt
    14161 gttgagcaga gcggaaatat ttttctgaat gaggtccgta tcgcggataa aaataccgcc
    14221 cattttcaaa ttaataacca gcgccgaaat caattttttt gtccagcggg gtttcatgct
    14281 gataatttcc agccaggcac gaatattcat gatatgggcc ggattcacct taacctgcca
    14341 ttcttcgttt gaacccttga tttccggata ctggaagccg taagcaatca actcgctgat
    14401 aaatgtattc gcaagctcat gactgttttg cgcgaaaact tcccgggccg ttatcgtaat
    14461 acaatcaaaa atggcgccgc tgaattcacg ctgagatttg cttttcttca aaaaaccgaa
    14521 aatcttgcgc acaaaatcat gaaggttttc ctgctgttcc tcctgaaaaa ccattttgag
    14581 cgaatgattg atttcacgca aggcgctggc gtgaatatca gcaaggctgg gaacctccat
    14641 gacctgaaaa agaaatcgca gcttggtgat atgctgatga ccctcgtagg ttttggattt
    14701 ttccagttga tccgcaattt gcagataacc gttcacaatc tggaaataat ccggcatctc
    14761 gagataattt ttgatcgcat cgtttttatc ttcatgggtg ttttgatagg acgtttccag
    14821 tttctccatc agcatccgca tggacgcgtg gctcagtggc cggatgaatt gtccgtaagc
    14881 agccaccgat tccggagatt cgccttcccc ggcaataaac cactgggagg gatctggttg
    14941 ttcaagccag aactgatacg taatccggaa aatcctgtag agcaaatcga cgaacagccg
    15001 gttttgcagc acatcgccat ggcccggaaa atttttgatg atttttttcg cataggacga
    15061 acattttttg aaaataattt cctgctgatt gatcgcatcc agaagagatt tgataatttc
    15121 cggaaacagc aacagatttc tggcggtttg ctcgttgctc tggttcagta tcgtatcgat
    15181 ataatcgaaa agataatgga ccgcgttgtc cttgacgtcc ttatccgatg acgatttgat
    15241 gacctcaaag taaatgcaaa gcatggtcgc cagtgcagaa aggccgtcct gatgtttatt
    15301 ataaatgtag aaatcgccga tggaaaccga tttcagttct tttaagacgt attcccagtt
    15361 gacaaaagga tgatggagct ccgttaaaag ttcgtagatt tttttctgca gaccgtaatg
    15421 gttttcgacc accttgagaa gcggcgcata ttgatccgga atttctatgg ttgccgccgt
    15481 tctttccaga ttcactttta gagcaaaaga atccagttcc tcgctttttt ttctgggatt
    15541 atcttttgtt gttgccatag ggggaacctt cggagagaag cttaatcaga atccgcgtat
    15601 tttttcaagt ccggaacaga tcgtacgaaa accagggggg gcgttacttc gaacatccgg
    15661 gttgcgctga aaaatcccta aaaaaacaaa ctccggcaat cccctcggag caacgagtgt
    15721 tatcggagtc gatatctatc ttcctgaaac gacactattt cttttttgaa gatttttttg
    15781 ctgttttctt taccgttgct tttttaacag cttttttcac ggttggtttc tttgcttttt
    15841 tcaatgccgg ttttttcttg ggcgctgctt tggttttttt cacgaccgtc ttttttctgg
    15901 gtgcggcaat ggcctgtgct tttttaggtt ttgccggtcc gagattgacc attggctctt
    15961 cacagcagat aagatcgatt gtggcacaac cacagtcttc atccagaatc accttcaatc
    16021 cgcatacttc acaacccaga acgtctccaa cctttgctct cgacattttc ttcctcctca
    16081 tggagagggg ttgaacatct tccctctggt attttttatt tcattctaat caaaaatatt
    16141 attttttcaa gcataaattg ctaatttttt tcaaaacata gacggcttga cattcattga
    16201 tgatttgata ttatcctccg gaaaaaggaa tccggatcag ggtatccaag ctcaccctga
    16261 caatcatcac caaaaagggc agcaaaaaat cgatcctcaa taagccgctt tcggaggggg
    16321 aaaaacgccg gactagcata tgccgcataa aaaaaaccaa aagtaccgca gctgggtgga
    16381 agttgatctc gacaacttta tcgccaatct gaaagaaatc aaaaggctaa tcggcgccaa
    16441 agccgatttt atgatgacgg ttaaagccga tgcttatgga cacggcgcca tagaaatatc
    16501 gaatatcgcg ctgaaatgcg gcgcacggat gctgggcgta gccaacgccg acgagggcgt
    16561 gcaattaaga atcagcggaa ttgaagcgcc tgtggttatc ctgggacctt ccacaaccgc
    16621 ggaaatcgaa cagatcatca aatataatct cataccttcc gtttcagatt tatcttttgc
    16681 aaaaaagttc aataacgccc tgatcaaatc ccatcataaa cgtcccttgc acatagaagt
    16741 ggataccggc atggggcgcg gcggcgcaat gcataccgaa gccgtaaaac tcgttctcga
    16801 tttgtccgga ttatccaaca ttatactgga aggcattttc agccactttg cttccagcga
    16861 aaaaatggtg tcccacaaca gcacgcaatg gaaatgtttt tcggatgtgc tggctgaaat
    16921 cgagtcccgg ggaatatata ttcccatccg tcacatcaac aacagcggcg ccattttgaa
    16981 ttatcccgaa tataaactca atatggtgcg gccgggaatt atggcctacg gcatatatcc
    17041 ttcgccggaa aacgaatcca aagccggact tttgccggtg atgtctttta aaaccggcat
    17101 cgttttgctg aaagactttc cggcggggca cggaatcggc tacaacagta cattcgttac
    17161 gtcacggccc acccgcatcg ccacgattcc tgtcggctac ggggacggtt atccgtttat
    17221 tctatccaac cagagcacag cgctcattca tggtcaaagg gctcccatcg tcggacggat
    17281 ttccatggat atgtgcacgg tggacgtcac ccatatttcc aattgcaaaa tcggcgatga
    17341 ggtcgttttg ctggggcagc aggaaaaaga atgcgtcagc gccaatgaaa ttgccgccaa
    17401 ggcacaaacg atcagctatg aaatcctctg cgccctggga aaaagagcgc cgcgtgtttt
    17461 tttgcaaaaa gggaaacaca aaaccgttga accgcggctt aagcggatat ttgtcccgga
    17521 agaggaacga tccctctcgc gtattgacaa catcattcgt cactgccttc tggcgcgcgc
    17581 acgcaacgaa gaactgggca acgccattta ttatgaaatg tttgaaaccc tttttggcaa
    17641 cagggaccgg cgtctcgaac tgcgttcggg tttccgctac gatatccgca tcgcgaaaat
    17701 gccgaaaacg aaagatgtcg ttctttccaa agactatctg catgtcggca tgcacgtcca
    17761 atacaaaaag acgctgcgcg acaatatttt catgatcggc tgcgcctcca gccattcgca
    17821 actcaacgcg ttatttaaag atcctcgctg tgaataccgc tggctattga acgacggcgg
    17881 aaacctctcc gggaaacgcg actttaccgt cgaacgggta cgcatcgata atgaagacgt
    17941 tcccatcatc gaaacaaaaa gcacaaaacg cggttatgag gtgtggtgcg ggtccgaccg
    18001 gctcaaaaag aaaataaatc aggatgtcaa acttgaaatg gacatatcca caaagcaggc
    18061 cagacaaaaa agaacttttc ccgtttatct ggtttatcct gtcagagggc ttgagataaa
    18121 atttgattac gcaaacgcgg gtatcaaaaa tgtccgcgag gaaagtttct ttgcaggaaa
    18181 acatccgcag ccttccgtaa cgggaaagaa aggaaaatcc atcgccgtta aaatatccga
    18241 cgaagaatgg attttcccga caagcggcgt catttttatt tgggacgact aagctcgagg
    18301 gtccggtcgt tatttgatgt agtaaccgga caccctccac cgcccatctt tgtcgaccat
    18361 cggcgtaacg gtctcaatgc ccgatttttt attctcgaat gacgtattga actgaatgac
    18421 aacatactgc ccgtcgggcg ctcccggcag ggacgttgtg tagagggcgt ttgtaacttt
    18481 tctgaaaatc aatttaccga gcggctcgcg tccggatttc atggcctgct cccactgctc
    18541 ctgcgaaacc gcccgcttaa aatagtcggc ggattccttc cagctttcaa tatatttccc
    18601 ctgatcaaca agtttcagcc acttttcagc cgatgcaatc gccgccttct ccctgtcgga
    18661 gctatcagcg acaacggccg tcacgccaag aaaaattcca atcacaacaa aacataatat
    18721 ctttctgatc atttatttcc tcctgttcat ccagcgacag acgtaatttc ctgcatgaat
    18781 tcgacgtttt ctaaaatgtt gtaaattcac tgacgggttc tttccttcag cagccagata
    18841 cccagagcgc ctagagcaac agcggcaacc gcgctgatcc agacgggaat ctccacgcct
    18901 tcggcggtca cgttgagacc gatgataaag cggccagcct gcgctactgc catgacaaaa
    18961 agtaaaatac ccacgaccaa cgatgccgga cgtttcataa ttttacctcc cgaaaaagaa
    19021 agttgctgtt ttctaaaaat aaaaagatcc tttgaacact caccaagacg ttgtaatctt
    19081 tacaacaact ttgtggagct gccccgacga tatcaggggc atatgcgcat cttccggaaa
    19141 aaaaattaca aatgaactgc ttcgtgttgc tatccaggaa tcaggctcat cagcaaaata
    19201 ctgtatatct tctccttcat catatgcgcc agttggttgt ttacacaaag atttaggctt
    19261 ccatcccatt gtgtctgttc ctgacagaac caactggata tcaatatatt tttcgtgcct
    19321 ctccaggaga gcctcctctt ttttacagcc ggaatctttc gataccatgg cgtaaacacg
    19381 ttcagcatct atttcatatt ttcccagcgc caggttcctc agatccggac gaaggagaaa
    19441 atctattgct cgggcgaatc cttgattcag ggctgaatat ttatgaagat tttcaataac
    19501 atccaaaacc atggattata tcccttcggt tttaatctcc aatttcttcg tatggcggtc
    19561 gatggctaat gtgattaatt tatccaagag aatgctgtat ttcaaaccgg tggcttccca
    19621 gagtttggga tacatgctga tgctggtgaa gccgggcagc gtgtttattt cgttgagata
    19681 gaatttattg gttttcttgt ctaggaagaa atccacccgc gccatggaag tacagcccag
    19741 tgccatgtat ccttcaacgg caatctttct gattttatcg gaaagggttt tgttgatttc
    19801 cgccggaatt tttagcgccg cgccgtcggg gtcaatatat ttggcttcat aggaataaaa
    19861 ttcgtgattc ggaatgatct cgcccaaaac cgaaccggcg ggattttcat ttcctaaaac
    19921 ggcgcattcg atttcgcggc aatcgattgc cctctcaatc atgacgcgtg tgtcgaactg
    19981 aaaggcgaag ttcatcgctt ttgcaagttc ttcctctttc ttcacttttc taacgcccac
    20041 ggaagaaccg gcgcaaactg gtttgacaaa aagcggcagc cccagtccgg acagcgcttg
    20101 tttcatgata actctgttat tctccttcca gtcctgtttg tttacagtga cagaaggaac
    20161 aacgggaatg cctgcctcgc aaagaagtct cttggcgatg tctttatcca tgcccacggc
    20221 cgaaccggtt acatctgcac ccacgtaagg cgccattgcc agctccaaaa gcccctgcaa
    20281 cgtgccgtct tcacagaacg ttccgtggat caccggaaaa acaacatcca caaccacttc
    20341 tttatttttg ttcttgatat cgtaaagatg cagtttgttc ctctgttcga aatggttgac
    20401 cagccacatg cccttttttt tcagagccat ttttttgccg aaatccttat cgggaataat
    20461 ttccggcttt tcctgcacgt accagcgacc gtctctgtcg atgccgatcg ctgtcacctt
    20521 gtacttcttg cggtccaggg cggcaaccac tgaagcggcg gaacaaagcg aaacgtcatg
    20581 ctcccccgac cggccgccgt ataaaacgcc tattcttatt tttgccgtct ctttcaaacc
    20641 cgtttttcca gccatattga tcctttcgat ggaagcgctt atttgtctta taattttcaa
    20701 gagttatacg ccttgagcga taaaattgtc aatttaattt tgcgattgat gattccgact
    20761 ttcttttttt aaccagcaga cgtcctgaac gaggggtctt gacatccata gtgtctatca
    20821 atatattacg gacgaactga aaatacctgc ggtgatttat gcccctttat tttaacgcgg
    20881 cgcaaatgcc cgtgacaaaa aaagctttta acgatgccga aaatctggta tcgcgtcagt
    20941 tcagattgag cgacgcagac ttcagaaaga atagatatga agtaaaaacg ctcgcttatc
    21001 ttgatgagca ggaaatcaaa gacgggacgt ttgctcatct ttgcaaatat acgtatgaaa
    21061 gaccggatgg cctggcaaac cgcagcgaga aggtctttaa tttttacaga atctgtttac
    21121 aggacaacat cattctggac gctgtggaaa gggccaatcc gtttattaaa ctcagtccgc
    21181 tgatgctcta catcgcggtc catgaactga ttcacatttt acgttttgca agcggcgaga
    21241 ttaaattcga tgccgatgcg aaggaaaagg aaaaagaaga ggtcattgtc aacaatttga
    21301 cccgcgcggc gctgctgccc gtcaaaggct acgatctgga aatcgtttta gactgcttca
    21361 gcagtcattt taaaaatttt gattcattca attaacggag gtaagttttt aatatgccta
    21421 tttacgaata caaatgccag aaatgcggga aacaatttga agcatttcag ggaatcaccg
    21481 atccggaatt aaaaacctgc aaattctgca aagggaaagt gcataaaatg atgtcccttt
    21541 cctctttcag cctgaagggt tcgggctggt atgtcaccga ctacaaagga aagaaaccgg
    21601 gagcggcgcc tgcaaaagga aaaacggaaa ctactgatag cgcggcaaaa acggaaacat
    21661 ctaaaaccga atcctctgaa agctaaacat acggaaatct tttttgatga ttttcgtgca
    21721 cagcgccttt gcatcttttg attgaatttt gttcaaggac cgtcagcaaa acgaattcat
    21781 ataatcatcc gcgaaacggc caacacaaat caacccgata agcgtcgctt atcgggtttt
    21841 aaattatgtg gaggagattt tttactcacc ccttttcccc tttgtctttc gacatcggct
    21901 cgtattccac ccggtctctg ccgttagtct tggctctgta aagcgccgta tccgcccggt
    21961 tgatcgcctg ctggatatct tcgccggcct gatattccgt aacacccacg gagatcgtca
    22021 ttttgaaatc gtcgggcata ttttcaaaca cgatcttctt gaccagattc cttattctgt
    22081 ctgcgcaaag gatcgcctga cttttctcca cattagtcag gattagaatg aactcttcgc
    22141 ccccgtaccg ggcaatgtga tcgatgttgc gtaaattctt ctgggtttcc tgcgccaccg
    22201 ttttcaggac aaagtctccg gcaacatgtc catatgtatc attcacgcgt ttgaaatgat
    22261 ccagatcgaa tatacaaatg gagaagggat gaatacctct atcgacaagg gccttttgat
    22321 attcgaggat tttaaacatc tgacgcctgt tgaagacctg cgtcagatca tcgatgattg
    22381 ccaacttttc aatggtggaa aaggcgtgtg atatccgcgc cctgagccgg gtaatgtagc
    22441 cgccgaccaa tgaaaaccag ggaagaacca gcgcaagcac cacaatgctc aaaacatccg
    22501 atgtgctgac caggtcagga ttaatgttat aaagcagaaa gatcaccagg gagtaattga
    22561 caacggcaaa ggcagaaagg aaaagaaact gccagacact caatctgaaa agtccgaaaa
    22621 taaaaacgac cagataggtc agcagaacca aaggcctcgt ggtgtccgcg taatacgcaa
    22681 tcaccatcac ccagaaggtg gcgataatca tttgaaggag cgtcagactg ggatctttga
    22741 atcttttgtt ataaccgctt ctgatcatgg catagatcag aacattgcag aacaatatgc
    22801 ccaaagaact gatgacaagc acctggatgt ttccccgagt catctgtccc tgcgcataaa
    22861 acatcacgca aatagcaaac cacatcaggt aagatcccaa cgccatgaaa aaacgtttca
    22921 atcgtaacgt ctgattgtgg tcgtcctgag gaaagatacc aagcattcgc atctcccttc
    22981 tgcttccaat attcagaaac gtttcatttc gttattatat aatccgcaaa acatcatgct
    23041 gtcaagcatt gaaatcattg cggaattttt cttatgaaac atcctcctgg cggagcagac
    23101 gatttacagc aggcttttga aatacggctg gagttcacgg cacaattctt ccggaacagt
    23161 cggacaaatg atttcattgt gcagtttttc aaaataattc aaatcgctcg ttcccaaagg
    23221 ataaatcacg aaacgcggaa cgattttccc ctgcacgcag aaataattat tattgttcat
    23281 ggtggcatag agagacaggt taaaactgat gaagttattg gtataaagat agctgaacac
    23341 acgggacagc cctgccagaa actcgctgaa attggattcg gtcagatcaa agaaggatgt
    23401 cttttcccga aaaatgaaat cgatttcccc ggccatgccc ttcggggcaa aactggtcag
    23461 ccagcagaca acacccgtat cggcaataaa ccgcttgtcg gcttttttct ccagcgcgat
    23521 taaattgctc cacagatttt ctccgttgga attcgcggcg taattttgcg cgctggataa
    23581 aagtttctgc ataaaacttg tcggacggta accggcaatg gtctgcaaat gcggatggat
    23641 cagaccgccc ccggcgggtg gcatgtagtt ccagttgatc gaacagtatt tgtatcccag
    23701 attcaattcg accacccggt gaaaatagtc ccggcaaacc agaaaaccgt cccgcatgaa
    23761 ttccggcgtc agttcattca tcgccacgag atgacgcgag gaaaaaatcg ccacgttgtt
    23821 gttctggtcg tagggaaaga cgttcggaaa gagacaggct tctcctcttt tgaaattacc
    23881 gtcttccata acagagggaa taaaacgggg ggttgtctta tcaaaaagat cggaacagaa
    23941 cgggcacaga gattccggag atttttccag atattcattc atatccggct tctgggcaat
    24001 gcgcatccgg taaggcagaa tacggcttgc cacgccagtg atctcgtcgt aacgcgactc
    24061 gatggtaatt ttcttctcct gaaaaccgtt gaacgggtca agataatatc cggtggttat
    24121 ttctttttta aatcccaaat ctctcataga attcctttct ttcaacacag gtcagttttg
    24181 atttttattt caagttgttt tctgttctct ccttaaactg ctgaaaagaa accgttcaac
    24241 gtttttttga gacaattccc cgccgagaat ttctttcaac gcactcagat ggatttccat
    24301 gcgtgcggcg ctccggtgtc cgccaccctt gccgaccgct ccgaaagctt tttgcgctat
    24361 cgccccgcag ttctgtctgt aaccgtcgcc gcggaaaata ataatcagtt tgtcctgaac
    24421 aattccggtg acgacaacgt aatacgtacc gatcaagtgc agataaaaat cggcgatttg
    24481 aacgcagaca tccgcgcttt cgacattgcc cagaaagcag atccgccgac cgcgaaaatt
    24541 gttcatgtga tgataagcgt aatgaaaata ttttaaaaac cggtcgggtg tttgattgag
    24601 ctcgatgcgc cggatcagcg gcatattggc gtattttgta tggtacgcgt acgcactgat
    24661 gtcttcaatt atcgtggccc gatcaaaatt gtccgtgtca gttttgatgc cgtagagcaa
    24721 tgccgtgtgc aggttgcggg gaatcttaat gcgggagcaa agcagatatt ccgtcaggat
    24781 gctggacagc gccccataat tcggcctcac atccatcagc tctgcgctcc actgcccttc
    24841 ataaggatga tggtcgaaaa caatctgcgg ccggagagca tccggaaaat gcgtgaaaaa
    24901 cgacggttgc gcatccagga gcgccgtcac acggtacgat gcaacatcca cctgattcat
    24961 gcgttgaata tcaagatgca gacaggacac caactcgata ttttgctgac ggtccagcgg
    25021 ttccgtggaa acaaaagcgc ttttggaaag tcccttggtc cgctgaagaa tttcgcgcag
    25081 cgccatcgcc gatgcgatgg catcgggatc gggactgccg tacatcaaca gcagcagcga
    25141 atcgtccggc tgaaccatgt ccatcagctt gtggaaatta agttccgtct cttttttaat
    25201 cgggatggcg ttgactttca tggtcccggc gtcttcaggg ccggttgatc ctcctcagta
    25261 aaccaatatt gtgccgggat gattcgggac aacttctccg acaacggcgg catcggctat
    25321 tcccgcacct cgcattttct cggcaaggtt ttgtgcttta tcgccgggca cactgaaaaa
    25381 cagtccgccg gccgtctgcg gatcaaaaag cagatcaaaa agccagtccg gacaggtttt
    25441 ttctttcacc acttgtttga tgcggaaatc tttgttgcga taaaggccgc ccggaacgaa
    25501 acccgtctcc atcaaatcgc gtacaccgtc cagagcaggt attgccgccg aatgaatgcg
    25561 cagaccgacg tcgcttcctt caatcatctc acaggcatga cccagtaagc cgaaaccggt
    25621 gatgtcggtg caggcgtgaa tatcgccggc ggcaatcatc agttgcgctg attttttgtt
    25681 gagcgtggtc atagtaacag ttacctgttg aatttgcttc ggtgatgctt caccggcctt
    25741 gatcgctgtg ctgataattc ccgttccgag agctttggtt aaaatgattt tatccccggc
    25801 tctggcgccg cgattgtata aaatgctgtc gggatgagcg actcccgtca cagataatcc
    25861 gtatttgatt tcattgtctt caacgctgtg cccgccgatc agcagggcac cggcttcatg
    25921 gattttattc aggccgccct gcaaaacgtt tcgcagaacc gaaacagcca tggtcttaat
    25981 cgggaaacaa acgatgttca tgaccgtcaa gggttttgca cccatcgcat aaatatcgtt
    26041 gagcgcattg acggcagcta cctggccgaa ggtgaacggg tcatcaaccg tgggcgtaaa
    26101 aaaatcaacg gtctgaacca gagccatatc agcggagatt ttatacactc cggcgtcttc
    26161 cgcgtgctca aatccgctca acagattcgg atcgtgaatc agcggcaaat cgcgcatgat
    26221 ttcggataag tccgccggac ctatcttgca ggctcaaccg gcgctctgca cggtttcggt
    26281 taaacggaca ccttttttct cttccatttt tcctcttaaa atcggttaca aaattttcca
    26341 aacattcacg aaataacgtt gaaagtataa ggacaacact tctgcatgtc aacgaaatct
    26401 tcatcacacc gttcagtctt tggtcaaata accttcagtc tgaatcgtat cttttcagtt
    26461 caaatatctt tttatcctgt ttggaaacaa tatgataaaa acggtcctga ggaatataaa
    26521 tgtaggggac atcgtcttcg cgccgataaa ggaagtacga caaaatcatt ctgtttaccg
    26581 cctgatggcc gacgatcata atgctgttgg cgtgccggtt caggaaaaaa gcctttttaa
    26641 tgccttccat gatgcggggc ttcatcagcg cgtaactttc gccttccggg taaatgtaat
    26701 tgtacttgtc cgctcccctt tttttgaaaa catgtggcat tttctgctcg atatcttcat
    26761 aagtcattct ttcgcagatt cccgcattga tttcatcaaa ttccttaagc ggaatgatcc
    26821 ggcagtcgtc ctgctgcgaa cggatgagtt tcgccgtatg aatcgttctt tgttttggac
    26881 tggtgaaaat ataagaaatt ttctttttcc tgaaaaaccg tcccagcgcg ctggcctgtt
    26941 tgagaccttt cggtgtcaat gccggatcgc cgccgattct gttttccaca ttatattccg
    27001 tttccgtgtg ccgaatcaga aaaagatttt ttacttcatc cgtgaccaga tagtcccgca
    27061 aacgttcata ataggggatc acgtcactgt gcttttcctc gacaatcatt ttctgaagcg
    27121 tgtcgatgcg gatataattt ctttccgctt ttaaaggcat gtagatcgct ttgtaataat
    27181 cgattctttt taaaaactcg cgggtcgcct tgtccttcag ataagcaaat tcgggaagac
    27241 gtatcttctc ctgaatgctc attttcagaa tatcgtcatc atcgttgacg cattcgataa
    27301 acaggaggtc gtggtcgttg agcgtttttt cgattaatcc gcgccgttcc cttcccgcgt
    27361 tcgtcgcatc cagaattgca atcatcccgt tgttgaacag gtatttcctg gctctgtcca
    27421 tgttcatccg tgcgaacttt tttctcaagg ccacggccgt ttcgttttcg ggagaataga
    27481 aatccgcgct ggatgtctcc agatgcggca gataaagcct gcgcaaattt ccattgttga
    27541 atatgcgggt gggaatccct tctttgagaa aataatccct gagacgtatg gccagcgttg
    27601 attttccccg cgcgggaagg cccattagaa caatatataa ctttttatca ctcatacctt
    27661 attgtttttc catgataata ttgtgtgaag tataggaaca atactttttt atgtcaatca
    27721 tggatatcat gtcctttcaa tggataataa aagggtcgcg gcattttttt gaattgattt
    27781 tttaatccaa ataagtttaa agacttaacc aagcacagca ggaagaaaag tatgcctatt
    27841 cgttattaca gtacaaaccg tcatttaaaa aatgttgcgg ggattacccc ctttaccggc
    27901 aaggtgtctt tcagggaagc cctgctcaag ggtcaggcct ccgatgaagg cttgtttatg
    27961 ccggatacca ttcctcaact ggccataacc gatattcagc agttgaatgg aaagccgtat
    28021 cgcgatacgg cactgcttgt cgccaaagcg tttcttgccg atgaatttcc accggacatc
    28081 cttcaacagg ttgtgaccga atcgtataat tatcctgtcc cgctggaaga ggtcaggccg
    28141 gaccatttcg tgatgcggct ggaccgggga ccgacagcgt catttaaaga ctttgccgca
    28201 cgcatgatgg ccaggctgat gagccactgc cgtccgaaag gcgaaaggct taacattctg
    28261 gtggctacat caggcgatac aggcagcgcc gtgggcgaag ccttcaaggg cgtaacagga
    28321 atcgacgtta ctatcttgta tcccgaaaat gaagtcagtg gcatgcagaa aaagcagctc
    28381 gacacaatcg gccaaaacgt gcaggcgttg agcgttgccg gcaaattcga tgattgccag
    28441 aatctggtca agcaggcttt ttccgatccg cggaatgaaa agtttaatct gacctcggcc
    28501 aattccatta acttcggtag gattctcccg cagatcatct actacgttta cgcctatgcc
    28561 cagcttgccc aaaacaaaga agaggcaatt ttttccgttc cgtcgggaaa tttcggtgac
    28621 gcgctgggtt gcgaatacgc ccgccgcatg ggtttgccgg tcgccaaact ggtcatgccc
    28681 accaatgaaa atgacgagtt tccgattttc atggcgacag gaaaatacaa taaggtttct
    28741 ccctcccgcg catgcatttc caacgcgatg aatgtcggac atcccagtaa tctggcgcgt
    28801 ttctttgatt tgtacggcgg cactgttgat cgcgttggac aggttcacaa atatcccgat
    28861 ctggaagaaa tgcgcagaca tattttttcg gtcagtgtca gcgattcgca aacccgggaa
    28921 accatcaaga acgtttataa acgctatcag gccgttctgg aacagcatgg cgctgtgggc
    28981 tgggcgggcc tggaagaata tttaaaaacc acacctgaac aaaagctggc gatctgcctg
    29041 gaaacagccc atccggcaaa attcccggag gaaattgaaa agcttttggg attaaccgtc
    29101 ccggtaccgg aaagcatgca aaacattgac ctgagagaag gctctgccgt tgccatgtcc
    29161 ggcgattaca gcagtttcag gaattatctg caaaagactt taaagataaa agaataaccg
    29221 aacgttgctg ttcatcgtaa gccgtgaaaa atttttctct gataatgtaa tgatcacttt
    29281 gatgacttta ttaaacatga gggagagata tcatgagcca caagaagatt gaacggaaaa
    29341 aagaattaga ccgccaaaga agaaggcgga aaaagagctt aaaagttcgc gcaaaagaaa
    29401 agagagataa tcacaagaag acaaagtaaa tatagcgaat actgaaggat gatttgttaa
    29461 taatacgaat atatcgcttc ctgtagcctt catttttcat atttacattt ttatgctagg
    29521 atacaacccg caatcgtcaa cgcattcagc attagaagcg atccatgtaa acaatgttgg
    29581 acaatggcaa taaaaattca attttataaa ggagtaaagg tatgttgaaa aaaatcgtta
    29641 tggccatttt gttcattatg atttctgcgg gttcctcgat ggcaggagga gccgtcgaga
    29701 aggattacgg caagaagccg tggatggtcg acatcgaaga tctgacagta aaaaatgaaa
    29761 atttccgtac cacgaagtgg acgggtcaga accttcagat gaccgttatg tcgcttaaac
    29821 caggtgagga gataggatta gaaaaacatg ataagggcga tcagtttatc cgtgttgaaa
    29881 aagggaatgc ccgtgttgtg atgggcgtgt cgaaggataa aatgactttc gacgaaaaag
    29941 tttcagatga ttgggccatt tttatcccag gcggtttctg gcacaacatt atcaacgaag
    30001 gggataagcc gctcaaagtt tacgttattt acgctccacc agaacacccc gctcgaacag
    30061 tgcacaagac tttcaaagaa tcggcagccg cccattctca ttgatcaggg aggaagggaa
    30121 agcatttgac aaggaaataa gtaataaaag aacgttgccc aaaacgctgc accggagtgt
    30181 aatttcgctg cgctccattg ccgtcggtga gtttggtcgt taaacctttc agatggtaat
    30241 caacaaaatt tattacccat aaaaaccgtc gaatagtagg taaactcaat aaaggataga
    30301 atttaagaca aggctttatt gtgagactca aatttcaaaa acgaataact taaaggtcac
    30361 tataggtgca ttgaaatttg tacgtcgtag tgagtcctaa atttaagaag catagcgtac
    30421 tgaaatttgt cggacgaact atccaatcct gataaacggg atggggaaca atcccgctaa
    30481 aagcggaggg tataacactc tgccccctgg ggccaaatta gtgtccagca ccaatagtaa
    30541 tagggattgc cctggtgttt atacccgtga tttaagaaat ttctcctcga tcaaatcggc
    30601 gatactttgg atgtcgttga tgtcgaagca gggaactccg gtatcgagtt tcgtatcgga
    30661 agcaatagcc agcaggttgt cctcttttcc gcataataat tcgcgtttca attccgaacg
    30721 gaagacttcg attttaggga aagggttgcc cttgtaacct tcggcaagaa tgacgtcggc
    30781 gcccttgata tatttatccc tgatttcacc gagcgtctga tcccgttcaa gatcttcaat
    30841 cagagcaacg cgtgtgggac aggcgatcac ggtcgcgcag gcgccggctc ttttgtgccg
    30901 ccagctgtct ttgccttcgt ggtcgatatc aaagccgtgc ttgttgtgct tgatggtcgc
    30961 gacgcgatac cctcttttaa tcagttcggg aatcagtttc tccaccaggg tggttttgcc
    31021 cgaattggat ttaccgacga tggatatgag cgagacagtc atttttacta ttttcctttc
    31081 aatcaacgca tcgccttatt tactacatcc tggtcgaaat aattacaaga cattcccgca
    31141 tccaccacaa cacctgtggc attaatgccg ccggaggcag gcgatagcag gaacaaaacc
    31201 gtgctggcaa cttcttcggt tttaagcgca gccttgcgca gcgtcaactg ctcggcaaac
    31261 agataattgt ttacatatcc cggaataccc gcggaggccg acgttttcaa aggaccggcg
    31321 cataccgcat tgacacgggc tttggacatt tcggataagg attttgccag aaaagccacc
    31381 gtcgcgtcga gcgcggcctt gatcggtccc atgtacccat aggcggttgc ccgcgtgttg
    31441 ctgatggata tggtgacaat ggaagcat
//
LOCUS       DAONQZ010000024        30419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1634_length_30419_cov_14.255698, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000024 DAONQZ010000000
VERSION     DAONQZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 30419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30419
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_30419_cov_14.255698"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="PLR13_05810"
     CDS             <1..1631
                     /locus_tag="PLR13_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HQC18886.1"
                     /translation="HAKAKRPILYKGYGDTWHLTTAERKRILLNNIYGVDIDTQAVEV
                     TKLSLLLKVLEDETSDTLNRQRVLFHERALPNLGNNIKCGNSLIGPDFYDGKQMNMLD
                     SEEAQRVNVFDWQAEFPEIFKAGGFDAVIGNPPYGALFNNEDKAYLGRKFESVVGKYD
                     SYGFFIEKGILLLQKEGCLGFITPHTWLTVIEAEALRKYLLTNATIESLVRLPTRVFS
                     NATVNTILAFVKKGKKLNNNKQCIRVLLLPRDIAISEIKESMGEVRHFTESSWATSEA
                     HMFNIEVGTKEQKLVNKIKQAGVLLTELCDFCVGVQAYDSHTGQERSIIDLRIYHSTT
                     KRDQTFRKELNGNDVSRYQIQWTEGNWISYGPWLAHPRQEYFFKGPRILVREITGSGR
                     YRIHAAFTDEDFVNYKTILNVIIKQESRSAGYHDFFILGLLNSELLSWYFPRSSNKLV
                     TKTFPRISILDMKRFTAPLINFSNKAEKKRHDNMVRLVEQMLALNKQLAEAKTGHEQT
                     LIQRQIDATDKQIDKLVYELYGLTEEEIAIVESK"
     gene            1877..2305
                     /locus_tag="PLR13_05815"
     CDS             1877..2305
                     /locus_tag="PLR13_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18887.1"
                     /translation="MPKYKYKQKLKEYIARHGKPDALTEYFILQGFSEDEIKEGLEPY
                     IEEWEEAIYWIGRDDGSEYDWDLWKRSSLYEVLRHATTEQIEPYRERIEKADEKFRSM
                     TYEVKDSYGDLLDKNETINRDMHWWLFRIPIHGQVWRVCR"
     gene            complement(2418..2678)
                     /locus_tag="PLR13_05820"
     CDS             complement(2418..2678)
                     /locus_tag="PLR13_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18888.1"
                     /translation="MKLDLFFASLIGAAVGCAVAYGIIRLKGIWILGALLYIAGGIMA
                     FLFAHIIFWHLVADTHYVFIPGTVGFCIWSVFIARLFFPRHV"
     gene            3170..3646
                     /locus_tag="PLR13_05825"
     CDS             3170..3646
                     /locus_tag="PLR13_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQC18889.1"
                     /translation="MTIYKRFVGTWKLKSVVRRSSSGVVSKPFGDDPLGYISYMPDGF
                     MHAILMKSGRGRIGVSPEELSDAAATKKLLISWKYLAAGLKYVKAMTSFLSYCGTYDI
                     CGNVVYHHVQAAMFPDWIGTDLTRTFVFDEDRLTLSARDDAGNVMDLVWERVSIMM"
     gene            complement(3697..5526)
                     /locus_tag="PLR13_05830"
     CDS             complement(3697..5526)
                     /locus_tag="PLR13_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQC18890.1"
                     /translation="MASNFLYSTREQKFILKEWLDLSKVFGAGRFQGGYSVDDIDSIL
                     ENAMKIAKLSIAPTSDDGDNLHAVYKDGKVTTPESYKKGYWAIQENGLGSSNADHTDP
                     SSMPLCVMGGTNEYLAAANASLGPYAMATTGSAGLIRDFGSENIKNIFLPKMFSGQWS
                     GTMDLTEPNSGSDVGDITTKATPTDEPGIYNITGTKCFITGGDQDITENIIHLALARI
                     EGCVSGTKGISLFVVPKYWPDANGEKGEFNDVNCGGIEHKMGLRGSATAIVNFGEDNK
                     CRGYLLGDPPVDGRGQGMAQMFQMMNEERTVTGLAAMSAATVAYNNACAYAAGRIQGR
                     QLTNPKAGRVPIINHEDVRRMLMFQKSITEASRAMIAFSYYCMDIAENTKDENERKLA
                     KAYIEVNIPIVKAWCSDMAFLSISEAMQCYGGYGFSEEYPIAQQLRDCRIYPIWEGTN
                     YIQSMDLIGRKWMMAGGQVFATWLGEIDKFVQANKANAVLGAQVALLEESLNTYREIQ
                     NTMAGYLGQGKLGMIGFFATRILHATGYIYGAKLLLEQALICQKKADELGKEHFEYPF
                     YAGKVASAKFFCHNILPVVSTILRVIKEGDNSVMEVPETTFAV"
     gene            5900..6523
                     /locus_tag="PLR13_05835"
     CDS             5900..6523
                     /locus_tag="PLR13_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQC18891.1"
                     /translation="MNVLLINGSPNKKGCTYAALSEVAKALKKQDIDAEIFYVGNKPI
                     RGCTACGKCSETGKCIFDDDAVNVAIGKAAEADGFVFGSPVHYAGASGFITSFLDRCF
                     FAGSDVFAYKPGAAVVSCRRGGATAAFDQLNKYFTISNMPVVSSQYWNMVHGNTPEEV
                     LQDLEGLQIMRTLGQNMAWLLKSIAAGRKAGIVRPEPEEHIWTNFIR"
     gene            6730..8175
                     /locus_tag="PLR13_05840"
     CDS             6730..8175
                     /locus_tag="PLR13_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC18892.1"
                     /translation="MQKNAEHIALISDKQHITYEQLHRAVGEVAGFMQQRGIGKGDKV
                     TVMLPNIPEFVFCYFGIVKNGAVVVPINPLTTPSELIYLLNNSDSKILITQSSQLKKY
                     HEIKDNLSTCRQVIAVDALSPEGQPFPGAESQGASFTPVEIKPEDPAVMIYTAGLTGR
                     PLGAVLTHHNLYSQAVVTQSIAQRTPDDVALALIPLFHAFGATANMLLTIRAGCSIVM
                     MDRLTMDGLFGSIEREKITYISAVPRLFMGMIFYEKASRYDTSSLKLCVTGGSAMPAD
                     FMHVFEEKFNLKLLEGYGLTEASPVCSFNRFDQVYKPGSIGTAITGVEIKIGDDQGRE
                     LPRNQIGELIVRGENVMQGYYKNEAATAAVIKNGWLHTGDLGRMDEDGYIFITGLKKR
                     MIITSGFNVYPLEVETVLNGHPAVKAARVSGKEDLMRGEIVKAYVILKEGAVADDKEI
                     IRYCKTYLSNYKVPREVEFVQEIPASADIEY"
     gene            8270..9211
                     /locus_tag="PLR13_05845"
     CDS             8270..9211
                     /locus_tag="PLR13_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQC18893.1"
                     /translation="MQKPTGFDFLKKHFIVIMGLAGFVFALAVTDMPTLRDYFHADNI
                     GESYAALQSRVQDHIPLPVVYGEKLNPESIIRLTNKARTDNGLAELAENRLLNQIAEA
                     RARDMLGKQYFAHVSPTGEQASDLAEDVGYAYKIIAENIGKGVFHSNQKIVEGWMQSP
                     GHRDNMLSEDIREMGAAVLEGKMKGRPTYVAVQIFGLQSLPTPHNICVAPSEDLLRDI
                     EVKKAQIAALQNQLDRLKNELDAQQAEIDTDKKYTYDDPQKIQNVNEKIYAYNEKSRW
                     YNKIVGEAKGKTAVLSSMIDEYNRAAQFYRACTEKTP"
     gene            9310..9858
                     /locus_tag="PLR13_05850"
     CDS             9310..9858
                     /locus_tag="PLR13_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18894.1"
                     /translation="MGRLTGAENYVLKKSDSNFLIALFAVIYFTSQIILGTIFHKIGT
                     LQAFMLQTTLSSDKFKDIASGWIASGQIGIYYQHFYLDYFHPLWYSIFLSLLVARAFK
                     MNHVNPKFNGVVLTPFIAGLCDLFENTMHIYFLADLDRATPVLVAISGLATNTKWILS
                     LSVTALAIVLIAYRIIKRRIIK"
     gene            9975..11213
                     /locus_tag="PLR13_05855"
     CDS             9975..11213
                     /locus_tag="PLR13_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC18895.1"
                     /translation="MNEKHTAHLKENYGKNILPLSVLENERMIAGSPNEILYFYRKDI
                     PIGKLKESIIKTIEHYNLFSSRLIAIGENKFALQYCTDGAVLNELPPLDAVSRDLSLE
                     DIKKMMVHVKTLPGEPLFAITGIPVKDGILAGISCSHTVADGISMLLFLYAWMCITEG
                     RPFLPPSPQRLFKGLPVDSDKIHRVFLPSLSELSREIQDKAGHYEKDEKLYTRREFFS
                     DEFLADMKRTAQQENETCSISSNQIIISFLLKKYHDHILRDTEKVRLRNGINLRDLHP
                     DIDPLHLGNATFTSMTEFTRKEIHAMSIAQIANRLKETIAGYRNEKFAKALSYLTEYG
                     IEYNAEVLRQHHKAYNSETDINSTNLTHLSDLESLGLSSNIGTILDVSSVVHAGFVIL
                     KEKNGQIFADVTSRYPFVQE"
     gene            complement(11182..11955)
                     /locus_tag="PLR13_05860"
     CDS             complement(11182..11955)
                     /locus_tag="PLR13_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQC18896.1"
                     /translation="MMIIGARAHNLDEANTLSKMHCPYIEISLDDPAVVSGWLPQLRN
                     ILNRDGITFLAHYPNEDNPFDVAILRNTFLPRIVTLMKLSQKLDIHKATIHFWIDKRR
                     LPHDLIRDKLDILSRIVDYGNDFGITVCIENLSERADSFAPAFDAIPDLKMTLDIGHG
                     QLLSRQNTSFRFIEEHFPRIAHVHAHDNHGGKTVSDDLHLALGDGSIDYKRIFKRLIA
                     KGYASTITTEVKPQDLLKSKQRLEDCIRSITPAQTGNGL"
     gene            complement(11990..12922)
                     /locus_tag="PLR13_05865"
     CDS             complement(11990..12922)
                     /locus_tag="PLR13_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQC18897.1"
                     /translation="MMNHRHPLHGRSTGMFRRIILCLALILAVASLVACSLINTLFPP
                     PAKPAKIALVLGGGGSKGFAHIGVLKVLEAQKIPVHMVVGTSAGSVVGSLYASGKSAF
                     ELQEIALKMDKDKIIDYDWKIWSGGLIIGDKLENFINTYVKNTPIEKLKIPFYAVATN
                     IATGEETVFARGNTGMAVRASCSVPAVFQPLKIGNNTYVDGGVVSPVAVNVARRNGAD
                     IVIAVDISGGLQRTVPDGMFDSIKKSVDIMYSKIAEYQIKHADVVIRPNMKHISSTEM
                     EKFNEAILEGEKAATLKLPELQQIMAQLKQERRL"
     gene            13188..15362
                     /locus_tag="PLR13_05870"
     CDS             13188..15362
                     /locus_tag="PLR13_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQC18898.1"
                     /translation="MQTVEKTSYKKDEKKEFTPDEFAKAKTTCEFVIELIKAISRSGY
                     YDSHHPVSQEVKKGLYIAFKKTLGDSSEIMLSCHEYEDKVDIHISGILNEPFNIRKLT
                     QSSTSDLFVPKLKDYFERKSLNSFVIKKNITAEHFESFIDVMSEPIAESADTSKLGEY
                     LTKSLVDRGITEVSTIFKTDIVLSRGKLPWRVSIILRRLAKDLNVVPMFRFATEDKMK
                     VIKKQIVEDIIRPLTNNDLLKDLIVNCDIVVDHVSHFMEVDELEKLIISSLQSDAVVP
                     ISTTVFDIYRDNLMDGVSQEENEQRLERNVYLAKVLNIAAERMIAEKMPDIKNLFEQL
                     FECKIIDSDMLPADMRIDRESAQLADEILSQTENYLENISKASSIEEAESIVLTVKRV
                     IPAFVNAGEWKIIARIVQALNDLSAHHEDMIQESEFLKGMPDSIWEGMEERIADEYIQ
                     GDANLRSKIDEVLLLSNTMCIKTVDLMISKSQEPNILKCAVEILSQKGETGRRWAMDV
                     LGQSNHPLSVLNAALLVIMQVGQKEDSKTVRKFLKHTNASIRARALAAMAKINQQDTA
                     AVIIDALNDEEEKVRHQALSLIEHELPVSVEMLQKIVSFLKERISKKNINANDAGLVC
                     GLLKAAGHLGEVSDRETLENEIIEMAAELLNEKKGILKFMKAEIPREKQDILCACAVS
                     LGKCGGIKSKEFLKTLSRNNNDAVANTAKDAMDILDQKAARQ"
     gene            complement(15402..18296)
                     /locus_tag="PLR13_05875"
     CDS             complement(15402..18296)
                     /locus_tag="PLR13_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18899.1"
                     /translation="MPTKHPIKSKNKKTSPYPNEFSLLSEEIIAKAGVGIYIVQNRKF
                     VYTSNLFQIITGFTDDDLIGASPLDRVYPEDQENVRQHAVKCLKKEIFEPYEFRLFNK
                     DNQLRWILETVTPIVYKGQPATLGSFMDITERKMTEEALRQSEEKYRTIIENIEDGYV
                     EMDLKGNFIFFNDAISKMHGFTSIELLKMNYTDVMDEENAAKIYAKYNHVFKTGIPEK
                     DFEYQIITKDGQRKFLETSISLINNSAGRIIAFRGIVRDRTQRKQAEEALRQSEEKYR
                     SILENIEEGFFEVDLAGNFTFFNHSLCSLLGYSREETLGMNYRQYTDKDSAQDVFRTF
                     NGIFRTGMPVKEFDWFIIRKNKTRRYVEASIYLKKDSSGKPTGFQGFTRDVTDRIQAE
                     FQKEAALEALRESEEKYRNILENIQEAYFEVDLAGNFTFFNDALCRMTGCSKEELRGT
                     NYTKFSDEENSKKVFQVFNKVFNTGQPTEGFDWLIIRKDSTKRYIEASVSLKKDSHGH
                     PAGFKGVIRDISERKRIEQELNYMATHDALTGLPNRLMFNQLLNQAILSAKRHKKQLA
                     VFFIDLDRFKIINDTLGHDAGDLLLQEIARRFRHSLRAVDVVGRLGGDEFVILIEDFS
                     DFAQVSKVAHKILSAAIKPMVIMSEECRVTASIGISIFPKDGQDEQSLMKNADIAMYF
                     AKEEGKNNFQYYSKDIKTQSNERLSIETNLRRALERDEFYLTYQARLDFKSNQITGVE
                     ALLRWNNPYLGQVTPTQFIPLAEETGLIVPIGRWVLKTACAQNMAWQKQNLPPICMAV
                     NLSLRQLSDENLLNDIRMALNESGMPPQLLELEITESMVMHNPAKLIQLLTKIKDMGV
                     RLAIDDFGTGYSSLAQIKHYPIDTLKVDRSFIRNLPKDSEDKAITEAIITMGKTLSLT
                     VVAEGVETLEQENYLREHVCDEMQGFYFSKPVKADDFAELLRNHTGAHLT"
     gene            complement(18442..18822)
                     /locus_tag="PLR13_05880"
     CDS             complement(18442..18822)
                     /locus_tag="PLR13_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HQC18900.1"
                     /translation="MMKKKIISSDTGPVSGPYSAAVEANDIIFISGQLPVDPATGKII
                     TDIKIATGQILTNMQALLKKTGLDLSHIVKTTIFLKDIKDFPAVNEVYAGFFPKDPPA
                     RSTVEVSVLPRDVPIEIEAIAVRE"
     gene            complement(18844..19749)
                     /gene="nadA"
                     /locus_tag="PLR13_05885"
     CDS             complement(18844..19749)
                     /gene="nadA"
                     /locus_tag="PLR13_05885"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQC18901.1"
                     /translation="MTIVDRINKLRQEKNAIILAHNYELPEIQDIADFVGDSLGLSME
                     AAKTDADIIVFCGVRFMAETAKILSPQKTVLLPDKDAGCAMADMIEPEGLRALQSRHP
                     GAVTMCYVNSSARVKALCDYCCTSANALPMTRKIAPDHRDIIFVPDQNLARYVSANAQ
                     CHFIIWEGYCPVHTDILPEHIQQAKRLHPQAKVIVHPECKPEITALADIVTSTEGMSR
                     HAQNSPDKEFIIGTETGILHRLKKENPDKFFYPASDKAICADMKKITLEKVLHSLETL
                     SYEITVAPDIMDKARLSIERMLQTT"
     gene            complement(19930..21051)
                     /locus_tag="PLR13_05890"
     CDS             complement(19930..21051)
                     /locus_tag="PLR13_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQC18902.1"
                     /translation="MNKIYKFFTLIIMTIALVIAAEETANAQIGWQKSAGQSLIDALK
                     VKEPLTFCNEKVPLKENDVRERLEREMLVSLQNSDDVILWLKRAYRYFPHIEMVLREN
                     RLPDDLKYIVIAESALKPLAASHKGAVGFWQFIESTGKRYNITVNSDIDERRNFFIAT
                     DAALAYLKDLYSIFNSWTLAAAAYNMGEDGLKTEILMQQTNNYYLLHLNQETQRYVFR
                     ILAAKIIMSDPEKYGFHLSKDDLYPPLQFDRVEISADNPVPIQVIAAAANTYFKIIKE
                     LNPHLKGYDLPPGRHSVLIPKGTADGFSGRYENLLNQWHIEKENATYTVQKGDSIYTI
                     AKNLNVSVKAIMIWNDLAGVNQKLSPGDKLVIFTRNRFF"
     gene            21206..22744
                     /locus_tag="PLR13_05895"
     CDS             21206..22744
                     /locus_tag="PLR13_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HQC18903.1"
                     /translation="MKKKIKPEAHSLFSGKDLQNMERLRLNLNDVERQLTLHRKGPLF
                     LKLNRPCTDHDGIVSFSSARRRALISFYERESCKYDIIKFVPASGAASRMFSEWFSAS
                     EKGGFGSKALDRLFVANLKKFPFFDLIRQNDPGRKLLAEKDVGRLLKFILSSGGMNFG
                     KRPKALIPFHRYSAREIRTPLEEHVCEAALYTLNRGVCHIHFTLSPGYKSGVARYLKK
                     VIAGYEKRNRIRYNISLSVQSASTNTIAVDKNNLPFRDDQGRIVFRPGGHGALLKNLK
                     HLDADFIFVRNIDNAAPETLWEKIIPHRKMLGGLAIRMQEEIFSHIRKLKNNKPDVSQ
                     IRNIANFCSEQLNIVFAEGFDRQPASEKIRTLLSCLNKPLRVCGMVRNENQPGGGPFW
                     VEEKDGTQTLQIVESAHVDTKQTDQAAIWFGAEYFNPVDMVCCIRDYRGRVFDLSDYV
                     NNDTYLITSKSEKGRQLKALELPGLWNGSMAYWNTVFVKLPIVVFNPVKTVYDLLRPG
                     HSVR"
     gene            complement(22768..23739)
                     /locus_tag="PLR13_05900"
     CDS             complement(22768..23739)
                     /locus_tag="PLR13_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HQC18904.1"
                     /translation="MTKAIALFSGGLDSILAAELVRRQTIDVLCLTLTTPFFNAQKAL
                     AASRQFNLPLEVMDITSEHLKMLRSPRYGYGKNMNPCIDCHTLMLNIAGRKMDQTGSD
                     FIVTGEVLGQRPMSQNKQSLNIVAKNSGYRDFILRPLSARLLEPTKAEKENLIDRSQL
                     LAIQGRGRKQQIILAADFGITSYAPPAGGCLLTDPVFTRRLRDLFAHQTEPEIRDYEL
                     LKHGRHFRIHDTCKIIVGRNSGDNEALKHLAASGDVLFNMAHFPGPLVVVPRDSLCDP
                     QIAAALCVHYSDAPPDALQEVICVRDNQSEIVTAAAASKEDCQRWIL"
     gene            complement(23943..25664)
                     /locus_tag="PLR13_05905"
     CDS             complement(23943..25664)
                     /locus_tag="PLR13_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HQC18905.1"
                     /translation="MFKRYVLALLIVLASTTLAWNIVEASREDTAQIQLKKTAISRKN
                     LHTYTVKEGDVLINIIKSIPGISEKDIPYTYRLIKDMNPHIANIENLEVGQSIFLPEK
                     PMKMKNDRTNVKAPAAAPKTVSAGEFYYKVKRGDTLYKIIRREFKVSETSIPQVVRSI
                     KVLNPTIKNVNKIYVGTMLKLPGKTVFVQSPDDLTPATRKPAISDTAEQYVQEDRIIE
                     IKDKKMMPFEARLALLKQIITQMNGTVTTTGNYYLPIPQAGQVTIDCSKIPLIEFDDN
                     TTVFLDLENRAHSNLKKMISNNWKNYHLVKISNTDDTITILKKVFSVTNKYSILKSDK
                     PLTFGALPPAEVMVDWLIVKPIPGQQSKSVIQGLRQIYENNVLLPKSIKNYAQKNGLI
                     ITEFSEETGIADKPEELYSLPPIPVFPATNAKEFSHALVSHLGLSAEKDVEIQIFDTV
                     KDGFNLSLKADVLVTKGNNKYVIYSQSLSQQFINALKQAGNETIFVSNSDSTKDIMGN
                     VLSGLDIPFTYGTHAFSGLEKNQAPYALKFSGTRINPDLYVVNFDIDPGLRGLLQEVW
                     SARIARY"
     gene            complement(25842..26711)
                     /locus_tag="PLR13_05910"
     CDS             complement(25842..26711)
                     /locus_tag="PLR13_05910"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQC18906.1"
                     /translation="MPPKPQPEDIAGGQALIEGVMMRHGDKIAAAVRRPDNEIVFQER
                     EYIPLTKRYKVLGWMFIRGTITLFEMMIVGFKALMFSADVALSEEERKPKDWEMYLSL
                     MISFSIAIFFFVVVPAYCFTKMKFYIDNLLLLNILEGCVRLGMFLCFLAATLLMKDMR
                     RVYMYHGAEHKTVFAWENGQDLTVENIKNFSTRHPRCGTSFILFVMIVSILVFSLLGR
                     PDFLHRVLYKILLLPVVSGISYEAIRFTGKYKHSRLVQILSWPGLMLQRITTREPSDD
                     QIEVAIAAMKKVI"
     gene            26851..27069
                     /locus_tag="PLR13_05915"
     CDS             26851..27069
                     /locus_tag="PLR13_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18907.1"
                     /translation="MAQMSSTDLFIREYQDRFEKKIRENEIASLEHWKAQLDKVIAMR
                     PDGVASMQLQITKISEMMSNRIKTLKKE"
     gene            27071..27646
                     /locus_tag="PLR13_05920"
     CDS             27071..27646
                     /locus_tag="PLR13_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC18908.1"
                     /translation="MDSIANFLFEVGMLSRTPRSGYQFLGSGQESVAEHILRTVFVGY
                     TLCKMDSSLDEMRVLKMCIFHDLPEARTGDMNYVNKKYVKVDEEKAIRELAESLSFGA
                     DIRSAIDEFNQKKTKEALIARDADQIALILQLKECGDLGNKYAEEWIGFAVQRLNTEN
                     GKKLSEKITGTDSSLWWFKEKGDWWVNGNNP"
     gene            27678..28124
                     /locus_tag="PLR13_05925"
     CDS             27678..28124
                     /locus_tag="PLR13_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18909.1"
                     /translation="MIHKKHRVSFFLLVLSVLNLISCFGLGPGGTVISKPDQITRTFE
                     TKETAALRAVARVFSEKKMGADTKIDYENKRVDSDYVVSGQWRTKAAAVVRQINWKEC
                     EVTVSVITEKHAEKGWEMRRLLQHPQYDNLFSVIELKIYEEMSRVN"
     gene            28145..28549
                     /gene="mscL"
                     /locus_tag="PLR13_05930"
     CDS             28145..28549
                     /gene="mscL"
                     /locus_tag="PLR13_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQC18910.1"
                     /translation="MAAKNKVSAMMEEFKNFAFKGNVVDLAIGVIIGAAFGKIVDSLV
                     KHIIMPLVSVVIPGEQGYLGWKWIINGKEVPYGLFIGEIVNFLIVAVALYFFIVKFLG
                     MMMKSKEEEAAGPAPLTKDQELLTDIRELLKK"
     gene            complement(28654..28839)
                     /locus_tag="PLR13_05935"
     CDS             complement(28654..28839)
                     /locus_tag="PLR13_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18911.1"
                     /translation="MATIMPEAENVQKAIKWISASLESENLPLQKLIEKAIFKFDLSP
                     KDTEFLTNFFRESRSNK"
     gene            29022..29594
                     /locus_tag="PLR13_05940"
     CDS             29022..29594
                     /locus_tag="PLR13_05940"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQC18912.1"
                     /translation="MKKSFGAKTLIYPTPVWLIGTYDQEGRPNAATVAWCGVCSSDPP
                     ALAISLRKSRYTYESIQHRKAFTVNIATADQVVAADYCGMVSGKKTDKFAAAGMTAVH
                     SNIVDAPYIAECPMVVECRLTDQMEVGIHVHLIGEICDVKVETTLLTEDQLPDIMKIQ
                     PLIYAPENRSYHGVGGYLGEAFSIGKKIMK"
     gene            29652..30230
                     /locus_tag="PLR13_05945"
     CDS             29652..30230
                     /locus_tag="PLR13_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQC18913.1"
                     /translation="MAKSIKGTKTEKNLLASFAGEAQARNRYTFFASAAKKEGYEQIS
                     RIFLETAENEKEHAKVFFKYLEGGEVEITASYPAGVIGDTISNLKAAAEGENMEWTVL
                     YSDAAKVAREEGFPDVARSFEQIAKVEMFHENRYRKLAENIAKGEVFKKKTATKWHCI
                     NCGYVFEGEEAPKECPACKHPQAYYEVLAENY"
BASE COUNT         8179 a         6606 c         7668 g         7966 t
ORIGIN      
        1 aacacgccaa agccaaaagg ccgatactct ataaaggata cggcgacacg tggcacctga
       61 ccaccgccga acgcaaacgg attctgctca ataacattta cggcgtggat attgacacgc
      121 aggccgtcga agttaccaag ctttcgctgc tcttaaaggt gctggaagat gaaacctccg
      181 atacgcttaa ccgccagcgg gttcttttcc atgagcgcgc gctgccgaat ttgggcaaca
      241 acatcaagtg cggcaattcg ctcattgggc cggatttcta cgacggcaaa cagatgaaca
      301 tgctggattc cgaagaagcc cagcgcgtca atgtgtttga ctggcaggct gagtttcccg
      361 aaatcttcaa agccggcgga ttcgacgccg tcatcggcaa cccgccgtat ggggctctat
      421 tcaataatga agataaagca tatcttggtc gtaagtttga gtcagttgtc ggcaagtatg
      481 actcatatgg tttcttcatc gaaaaaggaa ttttgttgct tcaaaaagag ggttgcctgg
      541 gattcattac tccacacacg tggcttactg tgattgaagc agaggcgctg cgaaaatatc
      601 ttctgactaa tgctacaatt gaaagtttgg ttagactacc gactcgcgtg ttcagtaatg
      661 ccaccgtcaa cacgatttta gcgtttgtaa aaaaagggaa gaaactaaac aataataaac
      721 agtgcatcag ggttcttctt ctgcctcgtg acattgcaat ctcagaaatt aaagaaagta
      781 tgggagaggt tcgccatttt accgaaagtt cgtgggcaac tagcgaggct cacatgttta
      841 acatagaagt tggtactaaa gagcagaaac tagtcaataa gataaagcag gccggagtgc
      901 tattgacaga gctttgtgat ttttgtgtag gtgtccaagc atatgattct cacacgggac
      961 aagaaagaag cattattgat ttacgtatat atcattcaac aacaaaaaga gatcaaacat
     1021 ttcgtaaaga gcttaatgga aatgatgtct cccgttatca aatccagtgg actgagggga
     1081 actggatatc ttatggtcca tggcttgctc atcctcggca agagtatttc tttaaggggc
     1141 ctcgcattct tgttcgtgaa attacaggtt ctggacgtta cagaatacat gcggcattca
     1201 cggacgaaga ttttgttaac tataagacaa tcctgaatgt gattattaaa caagagagtc
     1261 gcagtgcagg ttaccacgac ttttttatcc ttgggttgct gaattcagaa ctactttcat
     1321 ggtactttcc tagatcaagt aacaagttgg tcactaaaac atttccacgc atttcaatac
     1381 tcgatatgaa gcgattcacg gctccactaa ttaatttctc taacaaggca gaaaaaaaac
     1441 gtcacgacaa catggtgcga ctcgttgagc aaatgcttgc cctgaacaaa caactggccg
     1501 aagccaaaac cggtcacgaa caaacattaa tccagcgcca gattgacgcg accgacaaac
     1561 aaattgataa attagtttac gaactctacg gcctgacgga agaagaaatt gctattgtgg
     1621 aaagcaaatg aagaatcatc ggacgcaaga agtcccacgg cgctccgcgc ctgggatagt
     1681 tccacttacg tttcgagcag cactacgctt gctctcaacg agtgtcacta aaataaagat
     1741 agtcgaaggg aaataaaatt tggggcgtgc tcaggggagc acgccctaca aaaaaatgga
     1801 aacacaattt tgaaatgtag ggattgattc ccgatcaatc cgtaaaaact acgatgtttc
     1861 taagggattg ttaccaatgc ccaagtacaa atataaacaa aagctaaaag aatatattgc
     1921 caggcacgga aaacctgatg cgctgacgga atacttcatc cttcaaggtt tctcggaaga
     1981 tgaaataaaa gaggggctgg agccgtatat tgaggaatgg gaagaagcca tctattggat
     2041 tggaagagac gatggatcgg aatatgattg ggatctgtgg aaacgcagct cattgtatga
     2101 ggtattacgc cacgccacaa ccgagcaaat tgagccttat cgggaaagaa tcgaaaaggc
     2161 tgacgaaaaa ttcaggtcaa tgacatatga agtcaaagat tcttatggcg atctgcttga
     2221 taagaatgag accataaaca gagatatgca ttggtggctg ttcaggatac caatacatgg
     2281 ccaagtatgg agggtttgcc gataattatg agcaagatag gagaataaaa aacggcatgc
     2341 ggtggggcct cccacaaacc ccactttacg cgcccccgct ggaagcgggg tgctacggcc
     2401 gggagaatat taccgtgtta cacatgcctg ggaaaaaata agcgagctat aaaaaccgac
     2461 cagatgcaga agccaactgt gccggggata aaaacataat gtgtatcggc aaccagatgc
     2521 caaaaaataa tatgcgcgaa gagaaaagcc attattccgc cggcgatata aagcagcgcg
     2581 ccgagaatcc agatgccctt gagccggatg attccgtaag cgacggcgca gccgactgcc
     2641 gcgccgataa gagaggcaaa aaagaggtct aatttcatga tcgttgattc cccacgttgt
     2701 gcaatcgacg tgatgtaagg cgttttgccc gtgtccctaa ttcggctaca agtttagaca
     2761 atgcatccaa agaagtcaag aacgactttg gtaacacgag cagacaggac tctgctgagt
     2821 ttaagtttca gactgcactt gttaacaaga tgtccgaaac gatcatggag accgaaaaaa
     2881 aatcatgaaa aatcggcttg acatgtcgaa ttttcaaggt aggttcaatt ttgccgacaa
     2941 acaaatcgac aaactggtct acgaactcta cggcctgacg gaagaagaaa ttacgataat
     3001 tgaaggcggt aaataaatat ggattcccgc caaaaccatg cgggaatgac agtgcggggt
     3061 taattgttac ctacccaagt aaatgaaagt gtagggattg atccaccgat caatccgcaa
     3121 aaaaacggcg cgctctggag ttgcgccctg aaatttaggg atgagattta tgacgatcta
     3181 taaacggttt gtgggaacgt ggaaacttaa atcagttgta agaaggtctt cttccggcgt
     3241 tgtgagtaaa cccttcggcg atgatcctct cggatacatt tcctatatgc cggacggctt
     3301 catgcacgcc attctgatga agtccggacg aggccgcatc ggtgtttctc cggaagagct
     3361 cagcgatgcc gccgcaacaa agaagctttt gatttcctgg aaatatcttg cggctggatt
     3421 gaaatacgtc aaagccatga cgtcattttt atcctactgc ggcacctatg acatttgcgg
     3481 caacgttgtc tatcatcacg tgcaggctgc catgtttccc gactggatcg ggacggatct
     3541 gacgcgtacg tttgttttcg acgaagacag actcacgctg agcgcccgtg acgatgcggg
     3601 gaatgtgatg gatctggtct gggaacgcgt ttcaataatg atgtgaaaaa aaagagggac
     3661 ggggagaacg cccgtccctc ctgatgaacc tcggttttac accgcaaaag tcgtttccgg
     3721 aacttccata acggagttgt cgccttcctt aattactctc aaaatcgtac taaccacagg
     3781 caggatgtta tggcagaaga atttggcgga tgccaccttg cccgcataaa acggatattc
     3841 aaagtgttct ttgccaagtt catcagcctt tttctgacag atcagcgctt gttcgagcag
     3901 aagttttgcg ccgtagatat accctgtggc gtgcaggatg cgggtggcga aaaagccgat
     3961 catgcccagt ttgccctggc ccagatagcc ggccatggta ttttgaattt ccctgtaagt
     4021 gttcagcgat tcttctaaaa gagcgacctg cgcacccagc acggcattgg ctttgttggc
     4081 ctgaacgaat ttatcgattt cgccgagcca cgtcgcaaaa acctggcctc cggccatcat
     4141 ccacttgcgt ccgataagat ccatggactg gatgtaattg gtgccttccc agatcggata
     4201 aattcggcag tcacggagct gctgggcgat ggggtactct tccgagaaac cgtagccgcc
     4261 gtagcactgc atggcttcgg agatggaaag gaacgccatg tccgaacacc aggccttgac
     4321 gatggggatg ttgacctcaa tgtatgcctt ggccagcttg cgttcgttct cgtccttagt
     4381 attctccgcg atatccatgc agtagtagga gaaggcaatc atggcgcggg aggcttccgt
     4441 aatggatttc tggaacatga gcatgcggcg gacgtcttcg tggttgatga tggggactct
     4501 gcccgccttg ggattggtga gttggcggcc ctgaatacgg ccggccgcat acgcgcaggc
     4561 gttgttgtaa gcgacggttg ccgcagacat ggcggcaaga ccggtgaccg tgcgttcttc
     4621 attcatcatc tggaacatct gggccatgcc ctgtcccctg ccgtcaacgg ggggatcgcc
     4681 gagaagatac ccgcggcatt tgttgtcctc gccgaaatta acgatggctg ttgccgagcc
     4741 tcgcaaaccc atcttgtgtt cgatgccgcc gcagttgacg tcgttgaact cgcccttttc
     4801 gccgttggcg tccggccagt acttgggaac aacaaacagg gagatgccct tggttcccga
     4861 gacgcagcct tcaattcggg caagcgccag gtggatgatg ttttcggtga tgtcctgatc
     4921 gccgccggtg atgaaacatt ttgtgcccgt aatattgtag atgccgggtt catcagtggg
     4981 ggtcgccttg gtggtgatat cgccgacgtc ggagccgctg ttgggttcgg tcaggtccat
     5041 ggtgccggac cactgaccgg aaaacatctt aggcaggaag atattcttaa tattttcact
     5101 gccgaagtcg cggatcaggc cggcggaacc ggtggtcgcc atggcatagg gtcccaatga
     5161 ggcgttggct gccgccagat actcattggt gccccccatc acgcacagcg gcatggaact
     5221 ggggtcggtg tggtcggcgt tgctgctgcc gaggccgttt tcctgaatgg cccaatagcc
     5281 ttttttatag gattcgggtg tcgtaacttt accgtccttg tagacggcat ggaggttgtc
     5341 gccgtcgtcg ctggtggggg ctatcgacag tttggctatt ttcatcgcgt tttcgaggat
     5401 ggaatctata tcatcaacgc tgtagccgcc ctggaaacgt cccgctccaa agaccttgga
     5461 cagatctagc cattctttca ggatgaactt ctgttcacga gtggaataca aaaaatttga
     5521 tgccatgttt gtttctcctt cttgattgaa ctttatgttt atatccgaac aaacataatc
     5581 gaaagtatag aaactgccgg tcattttgtc aacgggttaa atccggggat ttgtagatgg
     5641 gcggggacaa atgacaaccg cccggaggct taaaaaaaag aaaggaaata ggatgaaatt
     5701 atataggttt tcacgtttaa tcatgaggaa gaattgtcat tttgtgcatt ggaaaccgga
     5761 caggggaaga aaaaaacaat cttttcgaat ggctcattgt attttttgaa agattggctt
     5821 agtatgaaaa aaagaaaaat aatcgtattc agacacaaag cgtatcggat gatgttcatt
     5881 aaaagaagag gtaggtaaaa tgaacgtatt attgatcaac ggcagtccca acaagaaggg
     5941 atgcacttat gccgctcttt ccgaggtggc aaaggcatta aaaaaacagg atatcgacgc
     6001 ggaaatattc tacgtcggca ataaaccgat cagagggtgt acggcctgcg ggaaatgttc
     6061 ggagacgggg aagtgcatct tcgatgatga tgccgtcaac gttgcaatcg gtaaagcggc
     6121 cgaggcggac ggttttgtct tcggctctcc cgtgcattac gcgggggctt ccggatttat
     6181 tacatcattt ctggaccggt gtttttttgc aggcagcgat gtctttgcct ataaaccggg
     6241 cgcggccgtt gtcagttgcc gccggggagg ggcaaccgcc gcctttgatc agctcaataa
     6301 atattttacc atcagcaaca tgccggtggt ctcgtcgcag tactggaaca tggttcacgg
     6361 caacacgccg gaagaagtct tgcaggatct cgaaggtttg cagatcatgc ggacgctggg
     6421 gcaaaacatg gcctggcttt taaaatccat cgccgcggga cggaaagcgg gaattgtccg
     6481 gccggagccg gaagaacaca tatggacgaa ttttatccga tgacccgatg ccgatattca
     6541 tgccgacccg aagaaacatg aataaatgat tagactattg cgggtaattg ttatacaaaa
     6601 aacgccggat gaaacgcgag cgaccggttt ttattttctt gaaaaatgat tcagggttat
     6661 gttaagtgac cggcaatttt ggggggaggt ttcagggcta ttaatctcat tcaattactc
     6721 gaagatgcgg tgcaaaaaaa cgctgaacac atcgctttga tcagcgacaa gcagcatatt
     6781 acttacgaac agctccatcg ggccgtgggc gaagttgccg gattcatgca gcagcgaggc
     6841 ataggcaagg gcgataaagt taccgttatg cttcccaata tcccggagtt tgttttctgt
     6901 tatttcggca ttgtcaaaaa cggggcggtg gttgttccca tcaatccgct gacaaccccg
     6961 tctgaattga tttatctcct gaataattcg gattcgaaaa ttttaatcac acaatcctct
     7021 cagctcaaaa aatatcacga aatcaaggat aatctttcca catgccggca ggtcattgcc
     7081 gtcgatgcct taagtccgga aggccagcct tttccgggcg cggagtcgca gggagcgtcc
     7141 ttcacaccgg tggagatcaa gccggaagat ccggcggtta tgatctatac tgccgggttg
     7201 acggggagac ctctgggcgc ggtgctgacg catcacaatt tatattcaca ggcggttgta
     7261 acgcaatcca ttgcccagag aacgccggat gatgttgcgc tggcgctgat tccgcttttt
     7321 catgctttcg gagccacggc caatatgctt cttaccatcc gggcgggctg ttccattgtc
     7381 atgatggacc ggctgacgat ggatggtctt ttcggttcca tagaaaggga aaaaattacg
     7441 tatatttcag ccgtaccgcg cctgtttatg ggaatgattt tttacgaaaa agcctcccgg
     7501 tacgacacca gttcgctgaa gctttgcgtc accggcggat cggcgatgcc ggccgatttc
     7561 atgcatgtct ttgaagaaaa attcaatctg aaacttctgg aaggttatgg actgacggaa
     7621 gcgtcgcccg tatgttcttt caatcgattc gatcaggttt acaagccggg atccatcggg
     7681 acggcgataa caggcgttga gattaaaatc ggggatgatc agggacgcga actgccccgc
     7741 aatcagatcg gcgaattgat tgtcagggga gagaatgtca tgcagggcta ttacaagaat
     7801 gaggcggcca cagcggcggt catcaaaaac ggctggctgc acacgggcga tctgggccgg
     7861 atggatgaag acggttatat ttttatcacc gggctcaaga aaagaatgat catcacgagc
     7921 ggatttaatg tttatccgct tgaagtggaa actgtgttga atgggcatcc ggcggtgaaa
     7981 gccgccagag tttcgggcaa ggaagatctg atgcgcggtg aaatcgtcaa ggcatacgtt
     8041 attctgaaag agggagcggt cgcggacgac aaagaaatta ttcgttattg caaaacctat
     8101 ctttccaatt acaaagtccc gcgcgaggtc gagttcgtgc aggaaattcc cgcttcggct
     8161 gatattgaat actaatggtt tttcaacggc gctgaacggg gcaagctgcg gttgaccttc
     8221 cctttctttc ttcatataat ttcacataaa aataaaggat gatgcagcat tgcagaaacc
     8281 gacaggattc gattttttga aaaaacattt catcgtgatc atggggctgg ccggttttgt
     8341 ttttgcgctg gccgttacag atatgccgac gcttcgggat tattttcatg ctgacaacat
     8401 cggggaatcg tatgctgctt tgcaaagcag ggttcaggat catattccgc tgcccgttgt
     8461 ttacggagag aaactgaatc cggaatccat tattcgtctg acaaacaagg cgcgaaccga
     8521 taacggcctt gctgaacttg cggagaatcg cttgttgaat caaatcgcag aagccagagc
     8581 gagggatatg ctgggaaaac agtattttgc gcatgtttct cccacgggcg agcaggcatc
     8641 ggacctggct gaagatgtcg gttatgccta taagattatc gcggaaaata tcgggaaggg
     8701 cgtttttcac tccaatcaga agatcgtcga ggggtggatg caaagccccg gacatcggga
     8761 caatatgctt tccgaggaca tccgggaaat gggagcggct gtgctggaag gaaaaatgaa
     8821 aggaaggccg acttacgttg ccgtccagat attcggtttg cagtcgttgc cgacgccgca
     8881 caatatttgt gttgcgcctt cggaagatct cctgcgtgat attgaagtca aaaaggcgca
     8941 gatcgcagct ttgcaaaacc agctcgaccg tctgaaaaac gagctggacg cgcaacaggc
     9001 agaaatcgat acggataaaa aatacacgta cgatgatcct caaaaaattc agaacgtcaa
     9061 tgaaaaaatt tacgcatata atgaaaagag ccgctggtac aacaaaatcg tcggggaagc
     9121 caaaggcaaa acggcggtgc tgtcctccat gattgatgaa tataaccgag cagcgcagtt
     9181 ttatcgggca tgcaccgaaa agacgccata gacggtttca tccaggcagc ttgtgattga
     9241 cagatcacgt caataagatt atattttttc cgttttcaaa agcatatcga aaaactccgg
     9301 aggccgatta tgggaagatt gacaggggct gaaaattatg tgctgaaaaa atcggattcc
     9361 aattttctca tcgcgttgtt tgccgtcatt tattttacat cgcagatcat tctcggtacg
     9421 atttttcata aaataggcac gcttcaggcc tttatgctcc agacaactct cagcagcgat
     9481 aaattcaagg acattgcgtc cggatggatt gcgtccggac aaatcggaat ttattatcag
     9541 cacttctatc tggattactt tcaccccctc tggtacagta ttttcctgag tttgctggtc
     9601 gcccgggcct ttaagatgaa ccatgtcaat ccgaaattca acggggttgt tctgacaccg
     9661 ttcattgcgg ggctgtgcga tctatttgaa aacacgatgc acatctattt ccttgcggac
     9721 cttgaccggg caacgcccgt tctggttgca atatcggggc tggccaccaa tacgaaatgg
     9781 attttgtctc tttcggttac ggccctggcc attgtcctga tcgcctaccg gattataaag
     9841 cgaagaatca taaaatagat ttaagtaatt gataaataag gaatattaat tagtagagcc
     9901 attgaacttt caatgttccg gatgtaacgg ttgttgctcg gatttaaaat tttatttacg
     9961 gagaaacgat gaaaatgaat gaaaaacata ctgcccatct taaagaaaat tacggcaaaa
    10021 atattctgcc attgtccgtt cttgaaaatg aacggatgat cgccggaagt ccgaatgaaa
    10081 ttttatattt ttacaggaaa gacattccca tcgggaaact caaagaaagc atcatcaaaa
    10141 ccattgaaca ttataatctc ttttccagtc gtctgatcgc gatcggtgaa aacaagtttg
    10201 cattgcaata ttgtacggac ggcgccgtgt tgaatgagtt gccgccgctt gacgctgttt
    10261 cccgtgatct cagtcttgaa gacattaaaa agatgatggt ccacgttaaa acattgccgg
    10321 gcgaaccgct ttttgctatt acgggcattc cggtcaaaga cgggattttg gccggcataa
    10381 gctgttcgca tacggtggca gacggcattt ccatgcttct tttcctctat gcctggatgt
    10441 gtatcactga gggcaggcct tttctgcctc catctcctca aagattattc aaagggctgc
    10501 ctgttgattc tgataaaatc cacagagtat ttctcccgtc gctttccgaa ctcagccgtg
    10561 aaattcaaga taaggccgga cattatgaaa aagatgaaaa attatatacc agaagggaat
    10621 tcttttcaga tgaatttctt gcggatatga aacgcacagc ccaacaggaa aacgagacct
    10681 gttccatttc cagtaatcaa attattattt catttttatt gaaaaaatat cacgatcaca
    10741 tcctgcgcga taccgagaag gtaagattaa gaaacgggat caatctgcgg gatctgcatc
    10801 ccgatatcga ccctctgcat ctgggcaatg cgacgtttac cagcatgacg gaatttacga
    10861 gaaaggaaat tcatgccatg tccattgccc aaatcgccaa tcgcctgaaa gaaaccattg
    10921 ccggttacag aaatgaaaaa tttgccaaag ccttgtccta tctgaccgaa tacggaatag
    10981 aatacaacgc tgaggtgctc aggcaacatc acaaggctta taatagtgag acggacatca
    11041 actcgacaaa tctgactcat ttgagcgatt tggaatccct tggcctgagt tcaaatatcg
    11101 ggacgatttt agacgtgagt tcggttgttc atgcgggctt cgttatcctg aaagaaaaga
    11161 acggacagat ctttgcggat gtcacaagcc gttacccgtt tgtgcaggag tgatgctgcg
    11221 gatgcaatcc tccagccgct gtttgctttt gagcagatcc tgtggtttga cttccgtcgt
    11281 gatggttgag gcgtatccct tggcaatcag tcgtttgaaa atccttttgt aatcaatgga
    11341 gccgtcgccc agggcgaggt gaaggtcgtc acttactgtt tttcctccat ggttatcatg
    11401 ggcgtggacg tgcgcgatgc gaggaaaatg ttcttcaata aagcgaaaag atgtgttttg
    11461 ccgtgaaagc agctggccat gtccgatatc gagcgtcatt ttcagatcgg gaatggcgtc
    11521 aaaggcgggg gcgaaactgt cggcgcgttc gctcaaattt tctatgcaga cggtaatgcc
    11581 gaaatcattg ccgtagtcca cgatacggga aagaatgtcc agtttgtcgc ggatcagatc
    11641 atgaggcaga cggcgtttgt caatccagaa gtgaatggtc gctttatgga tgtcaagctt
    11701 ttgagataat ttcatcaagg tgacgatacg ggggagaaac gtattgcgaa gaattgccac
    11761 atcaaagggg ttgtcttcat tggggtaatg cgccaaaaac gttatgccgt cccggttcag
    11821 gatgttccgc agttgaggaa gccagcccga aaccacagcg ggatcatcca gcgatatttc
    11881 aatatagggg caatgcattt tgctgagggt gttggcttca tcaagattgt gggcccgggc
    11941 gccgataatc atcataaata ctcgattgca gaatgctaaa aagaaagctt tagagacgtc
    12001 tttcctgttt taattgtgcc attatctgtt gcaattcagg aagcttcagt gtggccgctt
    12061 tttcaccttc gagaatcgct tcattgaatt tttccatttc ggtggagctg atatgtttca
    12121 tgttcggacg gatgacgacg tcggcatgtt tgatctgata ttcggcgatt ttggaataca
    12181 tgatatccac agattttttg atcgaatcga acatgccgtc cggaacggtt ctttggagtc
    12241 ctcctgaaat atccacggca ataacgatat ccgcaccgtt acgacgggca acattgaccg
    12301 ccaccgggct gaccacaccg ccgtccacgt aggtgttatt cccgattttc agcggctgga
    12361 aaacagcggg aacggagcag ctggcgcgaa cggccattcc cgtattgccg cgggcaaaaa
    12421 ccgtttcttc accggtggca atgttggtgg ccacggcata aaaaggaatc ttaagttttt
    12481 caatcggtgt gtttttgaca tacgtattga taaaattttc cagtttgtcg ccgatgatca
    12541 gaccgccgct ccatattttc caatcgtagt caataatctt atccttatcc attttcaggg
    12601 cgatttcctg caattcgaaa gcggatttgc cagacgcata gagactgccg acaacactgc
    12661 cggcgcttgt gcccacgacc atgtgcacgg ggattttctg cgcttcaaga accttcagca
    12721 caccgatatg cgcgaatcct ttggaaccgc cgccgcccag caccagggca attttggcgg
    12781 gtttggcggg gggagggaag agggtattga tcagagaaca ggcgaccagt gacgctacgg
    12841 caaggatgag cgccaagcaa agaataatcc tgcgaaacat tcccgtggaa cggccgtgta
    12901 atggatgtct gtgattcatc atttattgtg atcctcggag cctcaacgtt ttattttctt
    12961 acaacgtctt tcggtctttt gtcaacccgg ggtgttacag aaatcagacg ggggatgata
    13021 tttgtgattt gtatttcttt caagacatgt tatatataaa catctcatac attcaaacgg
    13081 caatgcgtcg aaaaaaaagc gtatccggca tcattgatcc gctttcttat gaatatagtt
    13141 ttaatcgatc tgttgttaca ccatatcaaa acgaaagggc tgatacgatg caaacagttg
    13201 agaaaactag ttataagaaa gatgaaaaaa aggaatttac tccggatgag ttcgccaagg
    13261 caaaaacgac ctgcgaattc gttattgaac tcatcaaggc catttcccgc agcggctatt
    13321 atgacagcca ccacccggtt tcccaggaag tcaaaaaagg cctctacatc gcttttaaaa
    13381 agacactggg cgattcttcc gaaatcatgc tttcctgcca tgagtacgag gataaggtgg
    13441 acatccatat cagcggcatt ctcaatgaac cgttcaatat aagaaaatta acacaatcca
    13501 gcacatcgga tctgtttgtg ccgaaactga aagactactt tgaaagaaaa agtctcaaca
    13561 gttttgtcat caaaaaaaat attacagccg agcacttcga gtcttttatc gatgtgatga
    13621 gcgagccgat tgcagaatcc gcggacacat ccaaactggg agaatacctc acaaaatctc
    13681 ttgttgacag ggggatcact gaagtatcga ctattttcaa aacagatatt gtcctttcca
    13741 gagggaaact tccctggcgt gtatccatca tcttgcgccg tctcgccaag gatctgaacg
    13801 tggtgccgat gttccgtttt gccaccgaag ataaaatgaa ggtaatcaaa aagcagattg
    13861 tggaagatat catccgtccc ctcacgaata atgatttact aaaagacctg attgtcaact
    13921 gcgatattgt cgtggatcat gtctcccact tcatggaggt tgatgagttg gaaaaactga
    13981 taatcagttc attgcaatct gatgccgtcg tgccgatttc cacgacagta tttgatattt
    14041 accgggataa cctgatggac ggggtttcgc aggaagagaa tgaacagcgg ctcgaaagaa
    14101 atgtttatct ggctaaagtt ctcaacatcg cggcagaaag aatgattgcg gagaaaatgc
    14161 ccgatataaa aaatttattt gaacaattgt tcgaatgtaa aattatcgat tcagatatgc
    14221 ttccggcgga catgcgtatt gacagggaaa gcgctcaact ggccgatgaa atattatcgc
    14281 aaacagagaa ttacctggaa aacatctcga aggcgtcctc cattgaggag gcggaaagta
    14341 ttgtcttaac ggtcaagagg gtcatccccg cgttcgtcaa tgccggtgag tggaaaatca
    14401 tcgcacggat tgtgcaggct ctaaacgatc tgtccgctca tcatgaagac atgattcagg
    14461 aatcggaatt tcttaaaggt atgcctgatt ccatctggga aggcatggaa gaaaggatag
    14521 ccgatgaata catccagggg gatgcaaatt tacgaagcaa aatcgacgag gttttgctgc
    14581 tgtcgaacac gatgtgcatc aaaaccgtcg acctgatgat cagcaagtcg caagagccga
    14641 acattttgaa atgtgcggtg gagatattat cccaaaaggg agaaacaggg aggcgatggg
    14701 cgatggatgt tctcggacag tcgaatcatc cgttatccgt gttgaatgcc gctttgctcg
    14761 tcatcatgca ggtcggacaa aaagaggata gcaaaacggt aaggaaattt cttaagcaca
    14821 ccaatgccag catcagagcg agagccctgg cggccatggc aaaaatcaat caacaggaca
    14881 ccgccgccgt cattatagac gctttgaacg atgaagagga aaaggtcaga catcaggctt
    14941 tgagtctgat cgagcatgaa ctgcctgttt ctgtagagat gctccaaaaa atagtatcgt
    15001 tcttaaaaga gagaatctca aagaagaata tcaatgcaaa tgatgccggg ttggtgtgcg
    15061 gtttattaaa agcggccggt catcttggag aggtttctga tcgcgaaaca ctggagaatg
    15121 agatcataga gatggccgcc gaattgctga acgaaaaaaa agggattcta aaattcatga
    15181 aagcggagat tccccgggaa aaacaggata tcctgtgcgc ctgtgccgta tcgctgggaa
    15241 aatgcggagg gataaaatcg aaggagtttt taaaaacgct ttcccgcaac aataatgacg
    15301 cggtagcaaa cacggcgaaa gacgctatgg acatcctgga tcaaaaagcc gcccgtcagt
    15361 gaaggacgtt gcgaacaggg cgcacttctt tttgcataat ttcatgtcag atgtgcaccg
    15421 gtatgattgc gcagcagttc agcaaaatca tcggctttga ccggtttgct gaaataaaag
    15481 ccctgcattt catcgcaaac gtgctcccgc aaataatttt cctgctcgag ggtttccact
    15541 ccttcggcaa cgacggtcag gctgagggtt ttccccattg tgatgatcgc ttcggtgata
    15601 gccttatcct cggaatcctt cggaagattc cggatgaaag acctgtccac tttcagtgta
    15661 tcgatcgggt aatgtttgat ttgtgccaga gaggaatagc ccgtgccgaa atcgtcgatt
    15721 gccagccgga cgcccatgtc cttgattttg gtcagcagtt gaatgagctt tgcgggatta
    15781 tgcatgacca tactttccgt gatttccaac tccagaagtt gaggcggcat gccggattcg
    15841 tttaatgcca tccggatatc gtttagcagg ttttcgtccg agagttgacg cagcgataag
    15901 ttgacggcca tgcagatggg cggcagattc tgcttctgcc acgccatgtt ttgtgcgcag
    15961 gctgttttaa gcacccatct gccgatggga acgatcaaac cggtttcttc ggcgagggga
    16021 atgaattgcg taggtgtcac ctggcccaga taaggattgt tccagcgaag cagcgcttcc
    16081 acaccggtga tttggtttga tttgaaatcc aatctcgcct gatacgtcag ataaaattcg
    16141 tcgcgctcca gcgcccgtcg aagattggtt tcaatcgaga gacgttcatt cgattgcgtt
    16201 ttgatgtctt tggaatagta ttgaaaattg tttttacctt cctctttcgc aaaatacatg
    16261 gcgatgtcgg cgtttttcat cagggactgc tcgtcttgtc cgtccttggg aaagatgctg
    16321 attccaatgc tggcggtgac gcggcattct tccgacatga tcaccatggg cttgatggcg
    16381 gcggaaagaa ttttgtgcgc cactttcgac acctgggcga agtcgctgaa atcttcgatg
    16441 agaatcacga attcatcacc gccaagacgg ccgacgacat cgacggctct taacgaatgc
    16501 cggaagcgtc tggcgatttc ctgtaaaaga aggtcgccgg catcgtgacc gagcgtgtca
    16561 ttgatgattt tgaaacggtc gagatcaata aagaaaacgg ccagctgttt tttatggcgc
    16621 ttggcggaga gaatcgcctg gttcagcaac tggttgaaca ttaaacggtt cggcaatccg
    16681 gtcaacgcat catgggttgc catgtaattt aattcctgtt cgatccgctt gcgttcagaa
    16741 atatcccgga tgactccttt aaaacctgca ggatgtccat gggaatcttt ttttagcgaa
    16801 acagacgctt caatgtatct ttttgtgctg tctttccgga taatcagcca gtcaaagcct
    16861 tccgtcggtt ggccggtatt gaaaacctta ttgaatacct gaaaaacttt tttcgagttt
    16921 tcttcatctg aaaattttgt ataatttgtt cccctcagtt cctccttgga gcaaccggtc
    16981 atccggcaca aggcatcgtt gaagaatgta aagtttccgg caagatcaac ttcaaaatac
    17041 gcctcctgaa tattttccag aatgtttctg tatttttctt cgctttcacg gagagcttca
    17101 agcgccgcct ccttttggaa ttcggcctga atgcgatcgg tgacgtcgcg ggtaaaaccc
    17161 tgaaaccctg tcggctttcc cgaggaatct ttctttaaat agatagaggc ctcgacatat
    17221 cgtctggttt tattcttccg gataatgaac cagtcgaatt ctttgaccgg catcccggtt
    17281 cggaagattc cgttaaacgt gcggaaaacg tcttgcgcac tgtctttatc ggtgtattgc
    17341 ctgtaattca ttcccaaggt ctcttctctg gagtaaccga gcagcgaaca gagcgaatga
    17401 ttgaagaaag taaaattgcc ggcaagatcc acctcgaaga agccttcttc gatattttcc
    17461 agaatgcttc ggtacttctc ctcgctttgg cgcagggctt cttccgcctg tttgcgctgc
    17521 gttctgtccc gcacgatacc gcggaaggca atgattcttc ccgcagagtt gttgattaac
    17581 gatatggatg tttccagaaa ttttctttga ccgtctttgg tgatgatttg atattcaaaa
    17641 tccttctccg gtatgccggt cttaaaaacg tgattgtatt ttgcatagat tttcgcggca
    17701 ttttcttcat ccatgacatc tgtataattc attttcagta attctatgct ggtgaaaccg
    17761 tgcattttgc tgatggcatc attaaagaag atgaaattcc ccttgagatc catttcgaca
    17821 tagccgtctt cgatgttttc gatgatggtt cggtattttt cttcgctttg gcgcagggct
    17881 tcttccgtca ttttccgttc ggtaatgtcc atgaaacttc ccagtgtcgc cggttgccct
    17941 ttataaacga tgggcgtaac ggtttccaga atccatctga gttgattgtc tttgttgaac
    18001 agccggaatt catagggctc gaaaatttct ttttttaaac atttgacggc atgttgccgg
    18061 acattttcct ggtcctccgg atacacacga tcgagggggg atgccccgat cagatcgtca
    18121 tctgtaaaac cggtaattat ttgaaaaaga ttgctggtgt atacgaattt ccgattctgc
    18181 acgatgtaga ttccgacacc ggccttcgcg atgatttctt ccgacaggag agaaaattcg
    18241 tttgggtacg gggatgtttt tttgtttttg ctttttatcg gatgttttgt cggcatcgtt
    18301 attttacctc tatgtatgga tagaaccgcg gttccatcca tatgcctgtt tgggaaatta
    18361 tcaaggtcgt cctgtggaga aatcggttat gaattaatct gtcttggacc gctccacgaa
    18421 ggagccggca gcggcaatgc attattccct gaccgctatg gcttcgattt ctatcggtac
    18481 gtctctcggc aatacgctga cttccactgt tgaacgggcc ggaggatcct tgggaaaaaa
    18541 tccggcatag acttcattga ccgccgggaa atctttaatg tctttcaaaa aaattgttgt
    18601 ttttacgata tgggacaaat ccaggccggt tttttttaaa agggcctgca tgtttgtcag
    18661 aatctgtccg gtggcgattt tgatgtctgt gatgattttc ccggttgccg gatcaacagg
    18721 aagttgcccg gaaataaaaa taatgtcatt ggcttccacc gctgcggaat aaggaccaga
    18781 gacgggacct gtatcggacg aaataatttt ctttttcatc atggcatcaa ccatccggca
    18841 atattacgtt gtctgtaaca ttctttctat ggagagcctg gctttgtcca taatatcagg
    18901 ggcaaccgta atctcatacg aaagggtttc caaagagtgt aaaacttttt ccaaagtgat
    18961 tttcttcata tccgcacaga tcgccttgtc cgatgcagga taaaagaatt tatccggatt
    19021 ttctttcttt aatcggtgaa ggatgcctgt ttccgtcccg atgataaatt ctttgtccgg
    19081 agagttttgg gcgtggcggc tcataccttc ggtggaggtg acgatatccg ctaaagccgt
    19141 gatttcgggc ttgcactcgg gatggacaat gactttggcc tgcggatgaa ggcgttttgc
    19201 ctgttggatg tgctccggta aaatatcggt atgtaccgga cagtatcctt cccagataat
    19261 aaaatgacat tgtgcgttcg cggaaacgta acgggccagg ttttgatccg gaacgaagat
    19321 gatatcccgg tgatccgggg caattttccg cgtcatgggc aacgcgttgg cggacgtgca
    19381 gcaataatcg cacaacgctt taacacgggc ggaggaattc acataacaca tggtgacggc
    19441 ccccggatgt ctggattgaa gggctctgag gccttccggt tcgatcatgt cggccatcgc
    19501 acaccctgcg tccttgtccg gcagcaatac ggttttttgc ggcgataata ttttggcggt
    19561 ttccgccata aagcggacac cgcagaagac aatgatgtcg gcgtcggttt ttgccgcttc
    19621 catgctcagc ccgagggagt ctcccacgaa atcggcaatg tcctgaattt caggcagctc
    19681 gtaattgtgc gccagaataa ttgcattttt ttcctgtctc agtttattga ttctgtcgac
    19741 aatggtcatg ggtgctcctg ctgtttatcg cgtctgtata aggtccctct gctttgacgg
    19801 cgattaaatc aaaacaaaag atttgtcaag aattaagagc ccccgctttt tcaggaatcg
    19861 atagccgtgc tgcttaaaaa tgcaaaagcg cacaggatac caagcgtcat cacagcggat
    19921 ggtgtgcaat cagaaaaaac ggttccgggt aaagatgacg agtttatcgc cgggagacaa
    19981 tttctggttg acgccggcaa ggtcgttcca gatcataatg gctttgaccg acacattcag
    20041 gtttttagcg atggtgtaaa tgctgtcgcc tttttgaacc gtatatgtgg cgttttcctt
    20101 ttcgatatgc cactgattca gcaagttctc gtagcgcccg gaaaatccgt cggctgtccc
    20161 tttgggaata agaacgctgt gacgaccggg gggaagatca taacccttca aatgcggatt
    20221 gagctccttg attattttaa aatacgtatt ggccgcagcg gcgatgacct gtatcgggac
    20281 gggattgtcg gctgagattt ccacccggtc aaattgcagc ggaggataca gatcgtcttt
    20341 acttaaatga aatccgtatt tttcgggatc agacatgata atcttggccg ccagaattct
    20401 aaagacgtat ctttgcgttt cctgattcag atgaagcaga taatagttgt ttgtttgctg
    20461 cattaaaatt tcagttttca gtccgtcttc acccatgttg taggcggcag ccgccagtgt
    20521 ccaggaattg aaaatcgaat aaagatcttt caaataggca agggccgcat ccgtggcgat
    20581 gaaaaagttg cggcgctcgt cgatatcaga attgacggtt atattgtacc ttttcccggt
    20641 gctttcaata aattgccaga aaccgaccgc tcctttgtgg gatgccgcca gcggtttcag
    20701 agcgctttcc gcaatcacga tatatttcag atcgtcggga aggcggtttt ctctcaaaac
    20761 catttcaatg tgcggaaaat atcgataagc gcgtttgagc cacaggatga catcatcgct
    20821 gttctgcagg gagacaagca tctctctttc cagtctttct ctgacgtcgt tttcctttaa
    20881 aggcactttt tcgttgcaga aggtcagggg ctctttgact ttcagtgcgt caatcagaga
    20941 ttgtccggca gatttttgcc agccgatctg agcgtttgcc gtttcttccg ccgcaatgac
    21001 caaggcgatg gtcatgatga tgagcgtaaa aaatttataa atcttattca tggccttatc
    21061 tccttgtttg atgcggggta tcacccggag gcaaaaaata tttattcaat ggttaaacgg
    21121 gaaaaagaaa catattcatt gaggtgatca taaagcaagt tgtttcctct tgtcaacgtt
    21181 tcatgttggt gttatgaaaa aaggcatgaa aaaaaagatc aaacccgaag cccattcgct
    21241 gttttccgga aaagatctgc aaaacatgga acgtttgcga ctgaatctca acgatgtcga
    21301 aaggcagttg accttgcacc ggaaggggcc gttattccta aaattaaaca gaccgtgcac
    21361 agatcatgac gggatcgttt ccttttcttc ggcacggcgc cgggcattaa tctctttcta
    21421 tgaacgggaa tcgtgtaaat acgacatcat caaatttgtt ccggcatcgg gtgccgccag
    21481 ccgcatgttc agcgaatggt tttcagcatc cgagaagggg ggcttcggat cgaaagccct
    21541 cgacagatta tttgttgcaa atttaaaaaa atttcctttt ttcgatctga tccggcaaaa
    21601 cgatccgggt cggaaactgc ttgccgaaaa ggatgtcggc cgtttgctga aatttatttt
    21661 gtcttccggg gggatgaatt tcgggaagcg tcccaaagcg ctgattccct ttcaccgtta
    21721 ttctgcacgg gaaattcgca cgcctctgga ggagcacgtg tgcgaagcag cgttgtacac
    21781 cctcaaccga ggagtatgtc atattcattt caccctgtcc cccggataca aatccggagt
    21841 ggcccggtat ctgaaaaagg ttatcgccgg atatgaaaaa agaaaccgga tcagatataa
    21901 tatctctctg tccgtacagt cggcgtcaac gaacacgata gccgtcgata aaaacaatct
    21961 tccgttcaga gatgaccaag ggcgtattgt gtttcgtccc ggagggcacg gggctttgct
    22021 gaaaaacctg aagcacctgg atgcggattt catttttgtc cgaaatatcg ataatgcggc
    22081 cccggaaacg ttgtgggaaa aaatcattcc ccacagaaaa atgctgggcg gtctggcgat
    22141 ccggatgcag gaggaaatat tttcgcacat acggaaactg aaaaataaca aaccggatgt
    22201 ttcgcagatc aggaatattg caaatttctg ttcggagcaa ttaaatattg tttttgccga
    22261 aggctttgac cgacaaccgg catcggaaaa gatacggact cttctttctt gtttgaacaa
    22321 accgctacgg gtttgcggga tggtccgaaa cgaaaatcag ccgggcggtg gtcctttctg
    22381 ggtggaagaa aaagacggga cgcagaccct tcagattgtt gaaagcgcgc atgtggatac
    22441 gaaacagacg gatcaggctg ccatctggtt tggcgcggaa tatttcaatc cggttgatat
    22501 ggtctgctgc atccgggatt accgggggag ggttttcgat ctctccgact atgtcaacaa
    22561 cgatacgtat ctgattacgt cgaaaagcga gaaaggacgc caactcaagg cgttggaact
    22621 gcccggttta tggaacggtt ccatggcgta ctggaatacg gtgtttgtga aattaccgat
    22681 cgttgttttt aatccggtca aaacggttta tgatcttttg cgtcccgggc attcggtccg
    22741 atagaatgtg tttcggatcc tacacgttta aagaatccat cgctgacaat cttcttttga
    22801 tgctgcggca gccgtgacga tttccgactg gttgtcccgg acgcagatga cctcctgcag
    22861 ggcatccggc ggcgcatcgc tgtaatgcac acataacgca gcggcgatct gtggatcgca
    22921 taggctgtcc cggggaacaa caacgagggg tccgggaaaa tgagccatat tgaacaggac
    22981 gtcgccggag gcggcaagat gtttcaaagc ctcattgtcg ccgctgtttc gtccgacaat
    23041 gattttgcat gtatcatgaa tgcggaaatg acggccgtgt ttcagtaatt cataatcacg
    23101 gatttccggt tcggtctgat gggcaaacaa atcgcgcagg cgacgggtaa acacgggatc
    23161 ggtcagcagg catccgccgg ccggaggagc ataacttgtg atgccgaaat ccgcggccag
    23221 gatgatttgc tgttttcggc cgcgtccctg aatggcaagt aactgtgagc ggtcgatcag
    23281 attttctttt tctgcttttg tcggttccag aagacgagcg cttaagggac gtaaaataaa
    23341 gtcccggtac cctgaatttt tggcgacaat attcagggat tgcttattct gcgacatcgg
    23401 gcgctgtccc agaacttctc cggtaacaat gaaatcgctc ccggtttgat ccattttccg
    23461 tccggcaatg ttcagcatca acgtgtggca gtcgatgcag ggattcatgt ttttgccgta
    23521 accgtaacgg ggcgaccgga gcatttttaa atgctcagac gtgatatcca tgacttccag
    23581 cggcagattg aactggcggc ttgccgccag cgctttttgt gcattgaaaa acggtgtggt
    23641 gagggtcaga cagagaacgt cgatggtttg tctgcggact aattccgcgg ccaggatgct
    23701 gtcgaggccg ccggaaaaaa gagcaatcgc tttggtcata aaattccaat cctttaacaa
    23761 tacattaccg gttgtttagc tgccgctgtc atgaaaaaca attttgagaa gaaaaacaag
    23821 aacaaaattg atgcgtcgag cgtttgccgg ttgagcggat cgacgaagca accgcagctt
    23881 cgaagcagat gttttaaaag cttcaatgat ttacgggcat gtccgatttt tcgagcaggg
    23941 gattaatacc gggcaatcct ggccgaccag acttcctgga gtaatccgcg cagtcccgga
    24001 tcgatatcaa aattgacgac atataaatcc ggattgattc tggtgccgct gaatttcaga
    24061 gcataaggag cctgattttt ttccagcccg gaaaatgcgt gcgtgccgta agtaaaagga
    24121 atatccaggc cgctcaagac gttgcccatg atgtctttgg tggaatcgct gttgcttaca
    24181 aagatggttt cattgccggc ctgtttcagg gcgtttataa attgttgaga gagagactgg
    24241 gaatatatga catatttatt gtttcccttg gtgacgagaa cgtccgcttt taaggaaaga
    24301 ttaaatccgt cctttaccgt gtcaaagatt tgaatttcga catctttttc tgcactgagt
    24361 cccaggtggg aaacaagcgc gtgggaaaat tccttggcat tggtcgccgg aaagacggga
    24421 atcggcggca gggaataaag ttcctcgggc ttatccgcaa tgcctgtttc ctcggagaac
    24481 tcggttatga ttaagccgtt tttctgggca taatttttaa tggatttggg cagcagaaca
    24541 ttattttcgt aaatctgccg cagtccctgg atgactgatt tcgattgctg tcctgggatg
    24601 ggtttgacga tcaaccaatc caccatgact tccgcgggcg gcaaggcgcc gaaggttaga
    24661 ggcttgtcgc ttttgagaat gctgtacttg ttcgttacac tgaaaacttt tttcagaata
    24721 gtaatggtat catcagtatt gctgattttg accaaatgat aatttttcca gttgttggaa
    24781 atcatctttt tcaaattact gtgagcgcgg ttttccaaat cgagaaaaac ggtcgtgttg
    24841 tcgtcaaatt caatgagcgg aatcttggag caatcgatgg taacctgtcc ggcctggggg
    24901 atgggaagat aataattgcc cgttgttgtg accgttccat tcatctgcgt gatgatctgc
    24961 tttagcaagg caagacgggc ttcaaacggc atcattttct tgtctttgat ctcaatgatc
    25021 cggtcttcct gaacgtattg ttcggcggtg tcgcttatgg ccggcttgcg ggttgccggc
    25081 gtaaggtcgt cagggctttg aacaaaaacg gttttcccgg ggagttttag catggtgccg
    25141 acatagattt tgttgacatt tttgatggtc ggattcaaaa ctttaatgct tcgcaccacc
    25201 tgcgggatgg atgtttccga gaccttgaat tcgcggcgga tgatcttgta taaggtgtcc
    25261 cctcgtttta ctttataata aaattcaccg gcagatacgg ttttcggagc ggctgcagga
    25321 gctttcacat ttgtccggtc gtttttcatt ttcattggtt tttccggaag aaagatggat
    25381 tgtccgactt cgaggttttc aatatttgct atgtggggat tcatatcctt gatgagacga
    25441 tacgtgtaag ggatgtcttt ttctgaaatg ccgggaatgc ttttgatgat attgattaaa
    25501 acatcccctt cctttaccgt gtaggtgtgc aggttttttc tggaaatggc ggttttcttc
    25561 agttgaatct gcgccgtatc ttcccgcgaa gcttcgacaa tgttccaagc caaggttgtt
    25621 gaagctaaaa cgatcaacag agccaaaacg taccgcttaa acatcagttc cttccccctg
    25681 gttgagatat tcagtgtata aatattgtat gaaaatactt taattggcaa taaaagtcaa
    25741 cttattctat ggctttgaga gaaagcggac ggtttcttcc gaaaaaaagc tggatggctg
    25801 accgaaaaat acattcgggc tgacgagacc tgaacaaaaa gttaaatcac ttttttcatg
    25861 gcggcaatgg ccacctctat ctggtcatcc gaaggttcgc gggtggtaat tctctgcagc
    25921 atgagccccg gccagcttaa gatctgcacc agtctggaat gtttgtattt cccggtaaaa
    25981 cgaatcgctt cataggatat tccggatacg acaggaagca gcaggatttt gtagaggacc
    26041 cggtgcagaa aatcgggacg tcccagcagg gaaaaaacca gaatggaaac aatcatcaca
    26101 aaaagaatga aacttgttcc gcagcgcgga tgacgggtgg agaaattctt gatgttttca
    26161 acggtcaggt cttgcccgtt ttcccacgcg aatacggttt tgtgttcggc gccgtgatac
    26221 atgtagacgc gacgcatatc cttcatcagc aaagtggcgg ccagaaaaca aagaaacatt
    26281 cccaggcgga cacatccctc aagaatattt aaaagcagaa gattgtcgat atagaacttc
    26341 attttagtga agcaatatgc cggaacaacc acaaagaaaa aaatggcaat cgaaaaactg
    26401 atcatcagag aaagatacat ttcccagtct ttgggttttc gttcctcttc cgaaagggca
    26461 acatccgcag agaacatcag cgctttgaaa ccgacgatca tcatttcaaa caacgtgatg
    26521 gttccgcgga taaacatcca tccgaggact ttataacgtt tggtcagggg aatatattcg
    26581 cgctcctgaa aaacaatttc attatccggt cttcggacgg ccgccgctat cttatcgccg
    26641 tggcgcatca tgacgccttc gatcagcgcc tggcctccgg cgatatcttc cggttgtggt
    26701 ttggggggca tataaccttc actgcaaatt tttgcgtctt ctttatagca tttaccgccc
    26761 ggtgtcaaat tatgattttg aaattcatgg ttgcaagata aacgtccgat gatataatgc
    26821 cgtaacttgc cgattaaaag gagatggaaa atggcgcaaa tgagttcaac ggatttattt
    26881 attcgagaat atcaggatcg cttcgagaag aaaatcagag aaaacgagat tgcttctctg
    26941 gaacactgga aagcccagtt ggacaaagtg attgccatga gacccgacgg tgtggcgtcc
    27001 atgcagttac agataacaaa aatatcggaa atgatgtcca atcggatcaa aacgctgaaa
    27061 aaggaatagc atggacagta tcgccaattt cttgtttgag gtggggatgc tcagcagaac
    27121 gccgcgcagc ggttatcagt ttctgggcag cggtcaggag tcggtggccg aacacattct
    27181 ccgcacggtc tttgtgggat atacgctttg caagatggat tcgtctctcg atgagatgcg
    27241 cgtcctgaaa atgtgtattt ttcacgatct gccggaagcc cgaaccggcg atatgaatta
    27301 cgtcaataaa aagtacgtca aggtggatga ggaaaaagca atccgcgaat tggcggaaag
    27361 tctttctttc ggcgcggata tcagatcggc aatcgacgag tttaatcaga aaaaaacaaa
    27421 ggaagccctg atcgccaggg atgccgatca aattgccctg attttacagt tgaaagaatg
    27481 cggcgatctg ggcaataaat acgcggaaga atggataggt tttgccgtgc agagattaaa
    27541 cacggaaaac gggaaaaaac tttctgaaaa aattaccggg acggattctt ctctgtggtg
    27601 gtttaaagaa aagggcgact ggtgggtgaa cggtaataat ccatgaagaa aacatcgccg
    27661 gcacataaaa cgattctatg attcataaaa aacatcgtgt gtcctttttc ctcctcgtcc
    27721 tgtcggtcct caacctcatc tcctgtttcg gcctcggacc gggcggcacc gtgatttcaa
    27781 aaccggatca gatcacgcga acttttgaaa cgaaagaaac agcggcgctt agagcggttg
    27841 cccgtgtgtt cagtgaaaaa aagatgggcg ccgacacgaa aatagactat gaaaacaaac
    27901 gggtcgattc cgattatgtg gtttcgggac agtggaggac caaagctgcc gctgtagtcc
    27961 gacaaatcaa ctggaaggaa tgcgaggtaa ccgtttctgt gattacggaa aagcatgcgg
    28021 aaaaaggctg ggaaatgcgc aggcttctgc agcatccaca gtatgataat ttgttcagtg
    28081 tgatcgagtt gaaaatttac gaggaaatgt caagagtcaa ttaaaacatt cagaggaggg
    28141 gaatatggcg gcaaaaaaca aagtatccgc gatgatggag gagtttaaaa attttgcttt
    28201 caagggaaat gttgtcgatc tggccatcgg tgtcatcatt ggagctgcct tcggtaaaat
    28261 tgtcgattcg ctggtgaagc atattatcat gccgctggtt agtgttgtaa taccgggaga
    28321 gcagggttat ctgggatgga agtggatcat caacggcaag gaagtgcctt acgggttgtt
    28381 catcggcgaa attgtaaatt ttctgatcgt ggccgtggcg ctgtattttt ttattgtgaa
    28441 atttctgggt atgatgatga agagcaaaga ggaagaagcc gccggacctg caccgttaac
    28501 caaggatcag gaattgctga cggacattcg tgaactgctg aaaaaataaa gcagggtcgc
    28561 tccacaaaca gattttcacg tcgttacggc gaagggctcg ggcaacttcg gttgcccgag
    28621 cgtgatttat ctgagtgaat ttgagagatg atattattta ttcgaacgac tttcgcggaa
    28681 aaaattcgtt aaaaattctg tgtctttggg agacagatcg aatttgaaaa tcgccttttc
    28741 aatgagtttt tgcaggggca gattttcgct ttccaaactt gctgaaatcc atttgattgc
    28801 tttctgaaca ttctccgctt ccggcatgat cgtagccata aaaaaccctc ctaaactgat
    28861 ttggaaaaca catttttaaa atgatgattc catgcatttt taattcatat actataaaat
    28921 aacacagaaa ttaatttaaa tttttggaat tttgatttat attttcaaaa tgaatgaaaa
    28981 aaataacaaa atccgctaaa ttgctttttg aggagattgc tgtgaaaaag tcatttggag
    29041 ctaaaacgtt gatttatccg acccctgtat ggttgatcgg aacgtatgat caagaaggca
    29101 ggccgaatgc tgcgaccgtt gcctggtgcg gtgtctgcag ttctgatccg cccgcgttgg
    29161 cgatttcact gagaaaatcg cgatatactt atgaaagtat tcagcaccgc aaggcgttta
    29221 cggtaaatat tgccacggcc gatcaggttg tagcggcgga ttactgcggc atggtgtccg
    29281 gtaagaaaac ggataaattt gcagcagcgg gcatgacggc tgtccacagc aacattgtgg
    29341 atgcgcctta tatcgcggaa tgtcccatgg tggttgaatg caggttgacg gatcagatgg
    29401 aagtcgggat tcatgtgcat ctgatcggag aaatatgtga cgttaaagtc gaaacaaccc
    29461 tgctgacaga ggatcaattg cccgatatca tgaaaatcca gcctttgata tatgctccgg
    29521 aaaatcgttc ttatcatgga gtcggcggat atctgggaga ggccttttcc attggcaaaa
    29581 aaattatgaa atgaccattg ctaagttcaa tcacagtgtt taacatatca aaaaagaaaa
    29641 ggagatgacg catggcaaag tcgattaagg gaacaaagac ggaaaaaaat ttactggctt
    29701 cttttgccgg tgaggcacag gcaagaaatc gctatacttt ttttgccagc gcagcaaaaa
    29761 aagaagggta tgaacagatt tcaagaatat ttctggaaac cgcggaaaat gaaaaggagc
    29821 acgccaaggt attcttcaaa tatcttgaag gcggtgaagt tgagatcaca gcctcttatc
    29881 cggccggggt aatcggagat acgatcagta atcttaaagc cgcggcggaa ggtgaaaata
    29941 tggaatggac cgttttgtat tccgacgcgg ccaaggtggc cagagaggaa ggatttccgg
    30001 acgtggcccg ttcttttgaa cagatcgcca aagtggagat gtttcacgag aacagatacc
    30061 gaaagctggc agagaatatc gcaaagggcg aagtctttaa aaagaagaca gcgacaaaat
    30121 ggcattgcat caattgcggt tatgttttcg aaggagaaga agcaccaaaa gaatgtcctg
    30181 catgcaaaca cccgcaggcc tattatgaag tgctggctga aaattactag tgagacaagc
    30241 tgagggcgag tgaaacgaag cacgaagcgt aagtcgaact atccctgtga gcgaagcgaa
    30301 tgggggtctt gagacaagct gagggcgagt gaaacgaagc acgaagcgta agtcgaacta
    30361 tccctgtgag cgaagcgaat gggggtcttg agacaagctg agggcgagtg aaacgaagc
//
LOCUS       DAONQZ010000025        29097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1735_length_29097_cov_16.296467, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000025 DAONQZ010000000
VERSION     DAONQZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_29097_cov_16.296467"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4157
                     /locus_tag="PLR13_05950"
     CDS             <1..4157
                     /locus_tag="PLR13_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HQC18914.1"
                     /translation="AKVLSWIMTVMFVLCAGTAWALPGGGAVVSGSDAPVVDDRTMTI
                     NQTAPKSIINWESYSIDRRETVAYTGQDTSWISLNRVTGADPSRILGQITADGAVWVI
                     NPNGLLIGSSAKINVGSFLGSTLNITDDNFNNAGTYSFRDGLNGIINQGKITTTNGGY
                     VALLAPSITNKGTITTPSGQTLIASGNAVNLHMDGNIIDGTGLNEATDSEALGIVNSG
                     KIYADGGSILLFANSAGDHLKTVINNTGVIQARTIATGEDKYKAGSIHLLGNFLGHGN
                     SNTINVGGTLDASAPNGGDGGSIETSAARVMVANNANVTTNAPQGATGYWYIDPVGDF
                     NIGASSGAGEGGSITGSKLGSSLGKTHVVIGTTHPGSINVNQPVSWRSNNTLTLNADE
                     YINVNKNITASGKNAGMEFNSDDGDFPEGPVADPLYFLQLNNGSVITLSGAEATLNLN
                     GDNYNVINSANVRFLDFQFTAPDLGENYALGTNINASVTSRWNGGEGFMPVGTEDDPF
                     TGNFTGLGHTISSLTINRPAAEGVGLFGCTEEGIISYVGLTGGSVTGREDVGALAGKI
                     RSDSYIFNSYATTKVTGNNEVGGLVGENEGLILNSYATGKVKGVTIDDDGLVRSEDVA
                     GLVGDNDGWIINSYAKGAVTGTDTVGGLVGENDGSILNSYATGAVSGVTVYFDDGFTV
                     HNYSEEIGGLVGENDGSILNSYATGAVSGDYAVGGLVGDNNDTIMGSVVDDTYEGYTS
                     FAIGKVSGFEDVGGLVGENDGSIWWSKASGAVNGSMTAGGLVGFNYGNILGSLATGAV
                     TANSNVMGAYAFGDTLFTVNGGAVLGGLVGVNEGSIKYSRADGAVSGGSIIGGLAGVN
                     DGSIFVAHANGNVTGSSTPSKITVETINDDLGYEPLLTDFTASIDGGNIVGGLVGLNT
                     GMIKKTDANGNVTGGSIVGGLVGLNVDSPCDEERGGKIMMSKATGNVKGGSNAGTIAF
                     DYNGPPPETVGGREGLSFIPVNTNITGSIKGGNIVGGLVGLNAGGKLKGVSAEGNVEG
                     GSIVGGLVGLNAGGQVRGALASGNVTGKNNVGNITLDYTEMPFAPGDVVDLPLTTANV
                     SIKGGNIIGGLVGLNFDSKIEYTLAEGNVTGGNFVGGLVGLNAGALCMGDGAVITDSA
                     AYGNVTGSKSTGAVNVSYNVSDSDEVFEDFNLNLKGGNFVGGLVGLNYDGGIINTHAD
                     GNVTGGGDYVGGLVGYNLNTVPEGDHPPTIGALIERSYALGNVTGGNFVGGLVGYNSG
                     VIVDTDAWGNVTGKDAVGGLVGFNDHDSVIVYSYSIGKVSGSTSVGGFVGHNNNGLIE
                     NAYWDMTTSGTIVGVGTSNGMPIDVTGLSTPDMKQESNFEGFDFDNVWYIDEGVTYPL
                     VGVLAP"
     gene            4276..6078
                     /locus_tag="PLR13_05955"
     CDS             4276..6078
                     /locus_tag="PLR13_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HQC18915.1"
                     /translation="MFPINRKNLFASVIIFFFFIALFALPAAAQERAGEIIQQFEKKE
                     VVPRDLPDMPVIEQKEEKTQEAPAGPKILIKQINVKMHTKTGGEEKALLLPPDTIKTI
                     TSRYENRELTFAEINQIANDITAAYRSRGYLLAYAFIPQQEVKDGLLEIGVIEAQLGK
                     ITVAGNTSYSTDFIRRHLEKIRKDPSIKTDTLQNSLLLLNEYPSLNVIAVMEAGKEFG
                     TTDIVAQVQDSRPVSGSISYDNFGSKYTSENRLNADLNIGNLLGSGDLLTLRGITGLD
                     EIDFDNLSYLRAEYAIPIMYYGTKMGAYYADSRYEVGEEYEPLEIHGKTHLAGLYLTH
                     PLLKTRLHALDLKLGFDYKDIYEYMLDKLASKDKIRVASLGATYNFIDKFQGRNIINL
                     TYHQGIRDLFGGTGKNERESKRQGLWEQLFGSSEINVTRTPTSRLYADGGFSKGTLDL
                     ARIQKLPGYNYMILKGSGQISSDNLFVAEQFFIGGISTVRGFSSASQGGDQGYCVSAE
                     FHLSPVFPEAKVFNQTFGDMFKFVLFADHGGVFKNDVLPGEDEDDYLTSIGAGLRLYM
                     GKHFFARLDYAVPRIDGSFETGDAEIYGQVAFVF"
     gene            6367..6807
                     /locus_tag="PLR13_05960"
     CDS             6367..6807
                     /locus_tag="PLR13_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18916.1"
                     /translation="MKHRIYFKALTFTGCLNLILPLMLLLILSACGMASSSQRVGGPC
                     TYKQYQGDAEIVSVTPVKGKAEEYEIRFSFHPREKIQEEFARVEGKQWLLVREDFSFP
                     KKDFLQQYGIRKGSRFPCVMNVITRGTCTPILFDFPTIRNGRAQ"
     gene            6868..8616
                     /locus_tag="PLR13_05965"
     CDS             6868..8616
                     /locus_tag="PLR13_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQC18917.1"
                     /translation="MKQKFLWPNITVFIILIFFILPVSGQADSTTSGQRERYVLDTNR
                     PVKTFPIMRPDDLTIRKWQSRFERAPRAHIREKYHRRAHHRAKARTDQTGKVSSINLL
                     GLLTYTPSENQGSCGDCWAWAGTRVMGIDLNTQQGSADNLSVQFINSCYGENACCGGW
                     LSDVVDFYNGKLMAISAANTNASFQDSAFRCSYFGAFSRVSCENITTEANYPIQSISE
                     ATVHTTGISQAEAISNIKNVLNQGKAIWFAFFLPDNASWNSFYNFWNNQSENALWDSD
                     NFCGQTWDTYEGGGHAVTLVGYDDSAADTDDHYWIALNSWGTTAGRPNGIFHVKMYIN
                     YNCTLRGLSSYPLGFQTLNIEWGVAQADPVDRTAPVVTAFSVPTVSKSLTVPVNVFTA
                     TDNMEVTGYMITASSAKPSSSAAGWSANAPTRYTFQSGTLGTKTLYAWAKDAAGNVSS
                     YKSASLTLSMPDLIVSAVSNWSTTITASRRSFSITDTNKNQGNAAAPVSVTKYYLSTT
                     PSRTANAILLGGTRSVKAIAVGKTSRGSAKVTVPQGIAKTVYYAIACADDNLVVNESS
                     ESNNCKASSRKITVKY"
     gene            8992..10152
                     /locus_tag="PLR13_05970"
     CDS             8992..10152
                     /locus_tag="PLR13_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18918.1"
                     /translation="MLVAFSFPSISFSATQEVVGQYCDVYTGDMKNKTALDDFKKSVN
                     EHARKDGFKKIVKASRLTNVTSDCINYAVSHYVDKVTVLSHTEKTENKGRRICEKVKM
                     TYDSESVSKYLSQEFCLQGWNANEEKVLWCYDVDTALESKEGKLNVGVIIEAQMANID
                     PGKKDLLEQEEEKQFLEMTQINKGKYQVLDRNAFMKAAEQQKMSLYGITDNDILKLGK
                     LLNLDVVVHRIINDNDRTTKVRKISTGKVLLAKTYVTESKAPAVAPDWIKYGKETNGT
                     VHFYKKGEAVKDSDESIIKVLNKWVFSKREVSNAIQYRRENRLSTKGYDKLSHMIYLS
                     EIDCQSKSERMIYLYLYDQDGKALYSHSYKTPEWNTIQLYSNGEALFKAVCR"
     gene            10273..11379
                     /locus_tag="PLR13_05975"
     CDS             10273..11379
                     /locus_tag="PLR13_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQC18919.1"
                     /translation="MIIRRSLGDVLAVSPVVDILARRFASASIDLLVNQDTAAAANLL
                     KGIRNIIAFDDGALKTTPVSSFMMQIGLLQKIFRRYDLAIAFTTNERSNLYAFFAGRT
                     SIGAREKVFAKNWWHRLLLDGGYLIDPQRHILENNLSALSTLGISIDRIVLSATYAGA
                     SGQKVQQMLKDFGMDRFIIFHPSGRYRCKSYPEQQRNSLLRLLDSLNIPIIVTGGHSD
                     IDRQVAATLPELKNVRSFIGRLSLEECAALTDLALAYVGVDTFNAHLAAAFNKPVFVM
                     FGPTIPEIWSPWSNDLQTAGRRNASMQIYGNITLFQSGMACVPCGHSKCRETDRPSPC
                     MEAIDPRNVFHQVQQWFLNLSPESQDNAKNVLPV"
     gene            11429..12100
                     /locus_tag="PLR13_05980"
     CDS             11429..12100
                     /locus_tag="PLR13_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQC18920.1"
                     /translation="MRALLDDPEKYLQQAEARTIQDNFKNKIVYFEIDKKKAVIKIHK
                     YKSAWHRIKRYFRQTRANRSWAYSLLFNAHGIPTPQPIAYKESRIGPLRGDSYFIYEW
                     VEGINGEEYFTKNKANPEIVEKAIDAIVDMTRTIKKLGLIHGDIRLKNMVFRGDDLLL
                     TDFDDTKKRQWYKSNYMNNRDFRGLIEDIYYNVPPEIQPLFLSRLESVGDDIRKLVRS
                     YKHRP"
     gene            12235..13260
                     /locus_tag="PLR13_05985"
     CDS             12235..13260
                     /locus_tag="PLR13_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18921.1"
                     /translation="MTATIAPAKLPVILLYNIDPGWTPEEKDEAIKQSAELGDALRCT
                     GHPTTLVTVTDNDIAEQLRPFDPAKYIVFNWCEELPGIPHSEWKVAKQLESLGYTFTG
                     ADSAALLLAQDKQRVKALLDQCAIPTPAWKIFDAPDPQGWKIFPAIVKPQNEHCSAGI
                     TPESVVLQPTDLQKRITFVLDHYAQPALVEDFIDGREFHVAVWGNETVNVLPAAEMDF
                     SRFGDLNDRLCTYEAKFTPGSLHYEEIKTLLPAPLTTGEKKSLETVCRNAYRASGCRD
                     YARMDIRLRDNIFYVLDVNPNADISSDASMACAAEADGISYAEMGSRIVEMAAKRHPV
                     LGKLFRS"
     gene            13317..14333
                     /locus_tag="PLR13_05990"
     CDS             13317..14333
                     /locus_tag="PLR13_05990"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HQC18922.1"
                     /translation="MNKKDFLKTKIEHIDIKKINAVDIVSAMKKMSFSARDLANAADI
                     YDRMLKEKNCAVILTLAGSTSAGGCMQVYVDLVKNNMVDAIVATGASIVDMDFFEALG
                     FRHYQGTPFVDDKLLRKLYIDRIYDTFISENDLQACDKTIKTIADGLAPRPYSSREFI
                     AEMGRYLTKNAKKNNSLVQAAYENNVPVFCPAFSDSSAGFGLVLHQHEHPGNHMSIDS
                     VRDFRELTDIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEILGYDVPMHKYAIQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATMALPLLASYAYHKGNWKKRKKWNLS
                     RIFK"
     gene            14668..17157
                     /locus_tag="PLR13_05995"
     CDS             14668..17157
                     /locus_tag="PLR13_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC18923.1"
                     /translation="MEDKNERTKLTERLFSSEDNNLPLLAKDIISNAGVAIYIVHNSQ
                     FVYVSELYQKLTGYAEKELIGEYSLKNIHPDDRDKVRKQAIKCLKKESFEPYEYRYIR
                     KDNEIRYILETVAPIIHKGERATLGSFMDITERKIIEEKYRNILENIQEGYFEVDLEG
                     NFTFFNNAVSRVLGYSREELMGMNNRQYTPDKDSARKVFQAYHHVYETGEPLNAVGWH
                     IITKQGAKRYIEGSISLLKDSQGKPIGFRGITHDVTERKVAEEALRQSEEKYRNILEN
                     IQEAYFEVDLAGNFIFFNDSLCRITGGTREELMGANYTRFSDPATSKNVFLAFNKIFK
                     TGEATEGFDWLIIRKDGSKKYIEASASLNKDASGKPIGFRGVIRDITERKRIEQELNH
                     MATHDALTGLPNRLMFSQLLDHAIQSAQRNKKHLAVFFIDLDRFKIINDSLGHEAGDT
                     LLKEMALRFKQSLRTVDIIGRLGGDEFTILVEDFNDLKQVEFLAKKILNTVMQPIALM
                     GEECRVTASIGISVFPKDGLDEQSLMKNADIAMYFAKSEGKNNFQFYSENIRSMSNER
                     LTIETNLRHAMERNEFYLDYQAKLDLQTRAITGVEALLRWNNPYLGLVAPTQFIPIAE
                     ETGMIIPIGRWVIKTACMQNVAWQKQGLPPVCMSVNLSLRQLMYENFAEDVKTILNET
                     RMASELLELEITESTVMHNPKRLVPILSKLKKMGIRMAIDDFGTGYSSLAQIKNFPIN
                     TLKVDRSFIRNLPQDTDNKAITEAVIFMGKSLKLKVVAEGVETREQAKFLKDQICDEM
                     QGFYFSKPIGPDGFADLLRENSVSGTDPGRQ"
     gene            complement(17203..17727)
                     /locus_tag="PLR13_06000"
     CDS             complement(17203..17727)
                     /locus_tag="PLR13_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HQC18924.1"
                     /translation="MALGIFEDEKPPRGIGGLIDWRLNGMLSREIKNGRIGGGFKEKV
                     VIPFPERIGSEILLLFGLGRVPDITYDRIYDVAYTVTQIIDSMAIKSFAFDLYGEGRA
                     NLVTSDIVEAAVTGIFDFLASDIEKLSEMNACAVTSPSNLHSVAQGVRQFKNNVNDKG
                     SVDISPLEECLAKN"
     gene            complement(17732..18187)
                     /gene="nusB"
                     /locus_tag="PLR13_06005"
     CDS             complement(17732..18187)
                     /gene="nusB"
                     /locus_tag="PLR13_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQC18925.1"
                     /translation="MCGRRKAREIALQVLYSLNFVDIPAQEALDLFWGNFVAPKSAKD
                     FAAFLVMGTCDHRKELDQLIAGCSDNWSLGRMSRVDINILRLAVFELLYCDDIPPKVT
                     MNEAIDLGKIFGSENSGSFINGILDALNLKLNKKNAAKSLDRKPRSSKT"
     gene            complement(18264..18728)
                     /gene="ribE"
                     /locus_tag="PLR13_06010"
     CDS             complement(18264..18728)
                     /gene="ribE"
                     /locus_tag="PLR13_06010"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQC18926.1"
                     /translation="MLKIIEGKTVAKGMKFGIVASRFNDFICGRLIEGTIDALIRAGA
                     EEKDIVIYKVPGSFELPLTAKKLAKSGRFDAVICLGAIIRGATPHFEYISAEVSKGIA
                     VVGLETEVPVVFGVLTTDTIEQAIERAGTKSGNKGADAAMTAIEMVDLFKKI"
     gene            complement(18776..19978)
                     /locus_tag="PLR13_06015"
     CDS             complement(18776..19978)
                     /locus_tag="PLR13_06015"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQC18927.1"
                     /translation="MAISTIQEAIEDIKNGKMIILVDDEDRENEGDLCMAAQFATAKT
                     INFMARYGRGLICLTLNEDMADKLHLKQMVQDNQCRFGTAFTISIEARHGVTTGISAA
                     DRATTIQAAVNPEAKPDDLVSPGHVFPIRAKKGGVLVRTGQTEGSVDLCRLAGLTPAG
                     VICEVMKDDGTMARMPDLEIFAKEHKLKIVTIADLIDYRMQNESLIKRMAEATLPTSF
                     GGDFKMIVYENEVDDWQHIALVKGDIKEDDEVLVRVHSECLTGDLFGSLRCDCGDQLH
                     RAMNMVEKEGKGIIVYMRQEGRGIGLVNKIKAYALQEQGKDTVEANIELGFKPDLRDY
                     GIGAQILADLGVRKMRLLTNNPKKIVGLEGYGIEVVKRVPIEIDPNENNIHYMKTKKK
                     KMGHLLKI"
     gene            complement(19997..20626)
                     /locus_tag="PLR13_06020"
     CDS             complement(19997..20626)
                     /locus_tag="PLR13_06020"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQC18928.1"
                     /translation="MFTGIVEGLGKVRRLTMKGADAVLDIDAGISLEDVSLGDSIAVN
                     GACLTVTSLQQNNFQADVSAETLSRTTLKLLQAGHKVNLEKSLRVGGFVGGHFVLGHV
                     DATARILSRMQKSGSLIIAVEMNDTISRYIVEKGSVAIDGISLTVNKLEKSRFYVNII
                     PHTAVNTTLVTRKEGEWVNIETDILGRYVENLLQSNKGIDKDFLVKHGF"
     gene            complement(20629..21090)
                     /gene="nrdR"
                     /locus_tag="PLR13_06025"
     CDS             complement(20629..21090)
                     /gene="nrdR"
                     /locus_tag="PLR13_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQC18929.1"
                     /translation="MKCPFCGHTENKVIDSRISKDGKAVRRRRECLGCTKRFTTYEYV
                     EDVLPMVVKKDGRREQFDRQKILTGIKKACEKRPISMEAIDKLVENIEQACQEMQAEE
                     ISSTVIGEKIMSELKNFDGVAYVRFASVYRQFRDVGEFMSELKDLLSKGKK"
     gene            complement(21111..21596)
                     /locus_tag="PLR13_06030"
     CDS             complement(21111..21596)
                     /locus_tag="PLR13_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HQC18930.1"
                     /translation="MMEIKSSAAVLREKKKKRPDWDSYFLDIVDLVSRRSTCCRRSVG
                     AGLVRDSRILATGYNGAPSKLQHCLDIGCLREQLKVPSGERHELCRGLHAEQNAIIQA
                     ALHGVSTKGSTLYCTNHPCVICAKMIINAGIIRVVIRDGYHDQLAAEMLKEAGIRVEQ
                     L"
     gene            complement(21630..22880)
                     /gene="glyA"
                     /locus_tag="PLR13_06035"
     CDS             complement(21630..22880)
                     /gene="glyA"
                     /locus_tag="PLR13_06035"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQC18931.1"
                     /translation="MSFLEKVDPEVMKAIDLETRRQAGKIELIASENFVSEAVLEAQG
                     SVMTNKYAEGYPEKRYYGGCEYVDIAESLAIERCKKLFGADHVNVQPHSGTQANMAVY
                     FAVAQPGDTILGMNLSHGGHLSHGSPANFSGKFYKIVPYGVNRETETIDFDEVEKLAK
                     EHKPKMIIVGASAYPRTIDFDKFRKIADEVGAVIMADIAHIAGLVCTGLHPSPVPVCE
                     FVTTTTHKTLRGPRGGVVMCKEAYAKTLNSRVFPGMQGGPLMHVIAAKAVAFKEALQP
                     AFKEYQAQIIKNAQAMADELRNQGFRLVSGGTDNHLMLVDLTDKNVTGKDVQDALDRA
                     SITVNKNGIPFDTKGPQVTSGIRIGTPAVTTRGMKEDEMRLIASCIADVIKNISSEQK
                     ITSVAQKIKVLCDRFPLYQGRLKN"
     gene            complement(23007..24266)
                     /gene="fabF"
                     /locus_tag="PLR13_06040"
     CDS             complement(23007..24266)
                     /gene="fabF"
                     /locus_tag="PLR13_06040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQC18932.1"
                     /translation="MKRRVVVTGLGALTPLGNSLHESWGAAIAGKSGIGLITKFDSTA
                     FKTKIAGEIKNFDPLQYVSKQEARRYDNFLLYSIAASEMALADARLTIGSDISERTGV
                     IIGSAIGGLATLEEEFKNLLDSGPRKISPFAVPAILANLASGQVSIRFGAKGPINCAV
                     TACASGTSAIGDAYKTIAYSDADVMIAGGVEAAVTQLAVGGFGSMRALSVRNDDPQKA
                     SRPFDKDRDGFIIGEGSGIVILEELSFALNRGARIYAELAGYGCTSDAFHMAAPPPGH
                     EGAARCMRVAIQDAGLNPEDIDYINAHGTSTPLNDLYETQAIKSLFGDHAKTLLVSST
                     KSMTGHMLGAAGGVEAIFAVKSLQESVIPPTINLDNPGEGCDLNYVPNTACRQAIHTA
                     MSNTFGFGGVNAVLLFKKFNESQFHKA"
     gene            complement(24290..24526)
                     /gene="acpP"
                     /locus_tag="PLR13_06045"
     CDS             complement(24290..24526)
                     /gene="acpP"
                     /locus_tag="PLR13_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQC18933.1"
                     /translation="MTLEEKVINIIVEQLDVTKEECVLEASFIDDLGADSLDIVELLM
                     EMEEAFGIEIADEELEKIATIQDVVDFLRQKGVQ"
     gene            complement(24693..24878)
                     /gene="rpmF"
                     /locus_tag="PLR13_06050"
     CDS             complement(24693..24878)
                     /gene="rpmF"
                     /locus_tag="PLR13_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQC18934.1"
                     /translation="MPNPVKRHSKTRRNQRRANDFLKSPSMSVCPQCNEPKMPHRICP
                     TCETYKGREYRKAEKND"
     gene            complement(24898..25428)
                     /locus_tag="PLR13_06055"
     CDS             complement(24898..25428)
                     /locus_tag="PLR13_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQC18935.1"
                     /translation="MKINVLLIPEEGQRFVFSEGDAWFKTCFHKEERLDFALETIDVD
                     CMITKTSGTVFIKGNFSALIDADCSRCLERTRLTIGSNFTYTLIPVKAEQKEEIELKP
                     EELEISSYQGDFIDLAPIICEQIILQIPIKILCREECKGLCQRCGTNLNAASCQCEKD
                     VRDTRMAVLKNFKIKN"
     gene            25672..27072
                     /gene="gltX"
                     /locus_tag="PLR13_06060"
     CDS             25672..27072
                     /gene="gltX"
                     /locus_tag="PLR13_06060"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQC18936.1"
                     /translation="MQKIRTRFAPSPTGFLHIGGARTALFNWLYARHLGGQFILRIED
                     TDRQRSTEESTKAILDAMSWLGLNWDEGPHFQAQRVQLHRDMVKKLIDEGKAYYCTCS
                     PEELEIKRKQALANGKKPKYDGTCRNRSLAKSANSVVRFRSRDDGATVVEDMIKGNII
                     FNNEELDDLIIERSDGYPTYNFAVVIDDALMNITHVIRGDDHVNNTPRQIQLYEALGF
                     DVPKFAHVPMILGSDKTRLSKRHGATSVMAYKELGFLPEALVNYLVRLGWAHGDQEIF
                     STAELIERFSMDAIGKSPAVFNPEKLLWLNAHYIKEASVERLMEEMKPLWPSVIDTGN
                     TDFIRKVIADFQPRVKTLMELVQSSDFYFADEIHYDKEAAQKFLTRDIVDHMKAIANT
                     ISSVQQFSKESLEEFFKTFTQTQNIKFKLIAQPLRVALTGKTVSPGIDEVMVTLGKER
                     VIKKINKAIAYIESLA"
     gene            27218..27292
                     /locus_tag="PLR13_06065"
     tRNA            27218..27292
                     /locus_tag="PLR13_06065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27251..27253,aa:Glu,seq:ctc)
     gene            28013..28420
                     /locus_tag="PLR13_06070"
     CDS             28013..28420
                     /locus_tag="PLR13_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18937.1"
                     /translation="MFNLDGKNIDGKHPIIFDFIYSRDYSDGYGYFKGDKEAISYAQA
                     VGKNQKMKFKKYGIVPKALVQMMAQYWSFQGKKNPSYRNVTYGEFLTLLINGPYKSEA
                     GKQVGEKFYRDLLIKDMPWIKRSFAMADKLYGR"
     gene            28600..>29097
                     /locus_tag="PLR13_06075"
     CDS             28600..>29097
                     /locus_tag="PLR13_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18938.1"
                     /translation="MTDKILTTKEGNDLPKPASEATESKAVVVPQPTAPMVPAEPETE
                     LWSYMGTDNTGFTDIAIVSVEDEDRQLIKTWMGKIKKTQKGQKISYENDIQEGISLIK
                     SISCEANKLINIANRNFADRAISIGMICHRLKQLIRGDKRPWAVWAEENLPFIAKRNR
                     EKYMLL"
BASE COUNT         7864 a         7137 c         6874 g         7222 t
ORIGIN      
        1 aagcaaaggt gctgtcatgg atcatgacgg tcatgtttgt cttatgcgcc ggcacagcgt
       61 gggcattgcc cggcggtggc gcggttgtca gcggctccga tgcaccggtt gttgacgaca
      121 ggaccatgac cattaaccag acagcgccaa aatcaattat caactgggag tcatacagta
      181 tagacaggcg ggaaaccgtc gcttatacag gtcaggatac cagttggata tctttaaacc
      241 gtgtaacagg ggcagatcct tcccggatat tgggacagat taccgcggat ggtgcagtct
      301 gggtcatcaa tcccaacgga ttgctaatcg gttccagcgc caagatcaac gtgggcagtt
      361 ttctggggtc gaccctgaac attaccgacg ataactttaa taatgcagga acctactcct
      421 tcagagatgg gttgaacggc attatcaatc agggcaaaat aactacgaca aacggcgggt
      481 atgtggctct gctggcgccc tccatcacca ataagggaac catcaccaca ccgtcaggac
      541 aaactttaat tgcatcaggc aatgccgtga acctgcatat ggacggcaac atcatagacg
      601 gcactggatt gaacgaagca acagattctg aagctttggg catcgttaac tcgggcaaaa
      661 tctatgccga cggcggtagc atcttacttt ttgcaaatag cgcgggtgac cacttaaaaa
      721 ccgtcattaa caacaccggc gtcatccagg caagaaccat cgccacagga gaggataagt
      781 acaaagcggg ctccatccat cttctgggta actttttagg tcatggcaac agcaatacca
      841 ttaacgttgg cggcacgctg gacgcttccg cgcctaacgg aggtgacggc ggttcgatag
      901 agacatccgc agccagagtg atggtggcca ataacgcaaa cgtaaccacc aacgcgccgc
      961 agggagcaac cgggtactgg tatatcgacc ctgtcggcga ctttaacatc ggcgcatcat
     1021 ccggtgcggg cgagggcggt tccatcaccg gctccaaact cggcagcagc ctgggcaaaa
     1081 cccatgtggt catcggcact actcatccgg gcagtatcaa tgtcaaccag cctgtctcct
     1141 ggcgttcaaa caacaccctg acactaaacg ctgatgagta tatcaatgtg aacaaaaaca
     1201 tcaccgcctc cggaaaaaac gcagggatgg agttcaacag cgatgacggc gatttccctg
     1261 aagggcctgt agccgatcct ttatactttc tacaactgaa caacggttcc gtcattacgc
     1321 tttccggcgc ggaggccact cttaacctta atggggataa ctacaatgtg atcaacagtg
     1381 caaatgtccg tttcctggat tttcaattca ctgccccgga ccttggtgaa aattacgcgc
     1441 tgggcacaaa catcaatgcg tctgttacgt cccggtggaa tggcggagag ggatttatgc
     1501 cggtgggcac tgaagacgat cccttcacgg gtaatttcac cggattgggt cacaccatat
     1561 ccagcctcac cattaaccgt cccgctgctg agggggtcgg cctgttcggt tgtaccgagg
     1621 aaggcatcat ctcctatgtc gggttaaccg gcggcagcgt caccggacgg gaagatgttg
     1681 gcgctctggc cggaaaaatc cggtccgaca gctacatctt caactcctac gccacgacca
     1741 aagtcacggg caacaacgaa gtcggcggac tggttggcga aaatgaaggc ctgattctta
     1801 actcctacgc caccggcaag gtcaaaggtg taaccatcga tgatgacggt ctcgttcgga
     1861 gcgaagatgt tgccggattg gtcggagaca acgatggttg gattataaac tcctacgcca
     1921 aaggtgcagt cacaggcact gacactgtcg gcggactggt tggggaaaat gatggatcga
     1981 ttctgaattc ctatgcgact ggagcggtca gcggcgttac cgtctatttt gatgacggct
     2041 tcacggttca taattacagc gaagaaatcg gcggactggt tggggaaaat gatggatcga
     2101 ttctgaattc ctatgcgact ggagcggtca gcggtgacta cgctgtcggc gggctcgtcg
     2161 gagacaacaa cgacacgatt atgggttcgg ttgttgacga tacgtatgaa ggctatactt
     2221 ccttcgctat cggcaaggtc agcggctttg aagatgtcgg cggactcgtc ggagaaaacg
     2281 acggcagtat ctggtggtcg aaagcctcag gcgcggtcaa cggcagtatg accgcaggcg
     2341 ggctggtcgg atttaattat ggcaatattc tcggatcact ggctaccggc gccgtcaccg
     2401 caaacagcaa tgtcatgggt gcttacgcgt tcggcgatac cttgtttacc gtcaacggtg
     2461 gagccgttct cggcggactg gtcggcgtta atgagggcag tataaaatat tcgagagctg
     2521 acggagccgt cagcggcggc agtatcattg gcggtctggc cggagttaat gatggatcga
     2581 tatttgtggc tcatgccaac ggcaatgtca ccggaagcag cacccccagc aaaattactg
     2641 tcgaaacaat caacgacgat ctgggatatg aaccgttact cactgatttt acagccagca
     2701 tcgatggcgg caacattgtc ggcggcctgg taggactcaa caccggcatg attaaaaaaa
     2761 ccgacgccaa cggcaatgtc accggcggca gcatcgtcgg cggactcgtc gggcttaatg
     2821 ttgattcacc ttgcgacgaa gaacgcggcg gaaaaatcat gatgtcgaaa gccaccggca
     2881 acgtcaaggg cggcagcaac gcaggaacca ttgcgtttga ttataacggc ccgcctcccg
     2941 aaaccgtagg tggtcgcgaa ggcttgtcgt ttattcctgt taataccaac atcaccggca
     3001 gcattaaagg cggcaatatt gtcggcggac tcgtcggact caatgccggc ggcaagctga
     3061 agggtgtctc agccgaaggc aatgtcgagg gcggcagcat cgtcggcgga ctggtcggac
     3121 tcaatgccgg cggtcaggtc aggggtgcgc tcgcaagcgg caatgtgacc ggtaaaaaca
     3181 acgtcgggaa catcaccctt gattacaccg aaatgccttt tgctcccggt gacgtcgttg
     3241 acctgccctt aacaactgcc aatgtcagca ttaaaggcgg caacattatc ggcgggctgg
     3301 tcggactcaa tttcgacagc aaaatcgaat acacattagc cgaaggcaat gtcaccggcg
     3361 gtaactttgt cggcgggctg gtcggactca atgcgggtgc tttgtgcatg ggtgacggcg
     3421 cagtcatcac cgattcagct gcttacggca atgtcaccgg cagcaagagc accggcgcgg
     3481 tcaatgtcag ctacaatgtt tcggacagcg acgaggtctt tgaagatttc aacctgaatc
     3541 tcaaaggcgg caactttgtc ggcggactcg tcggactcaa ttacgacggc ggaatcatta
     3601 acacgcacgc tgacggtaat gtcaccggcg gcggcgatta tgtcggcgga ctggtcggtt
     3661 ataatctgaa cactgttccg gaaggtgatc atccgcccac cattggagcg ctgatcgaaa
     3721 gatcctatgc tctcggcaat gtcaccggtg gcaactttgt cggcggcctc gtcggctaca
     3781 acagcggcgt catcgtcgac accgatgcct ggggtaacgt gaccggcaag gatgcggtcg
     3841 gaggactcgt tggattcaat gaccacgaca gtgtgattgt ctattcttat tccatcggca
     3901 aggtcagcgg cagtacatcc gtcggcgggt ttgtcggtca taataacaac ggtctgattg
     3961 agaatgccta ctgggatatg acgacatccg gcacgatcgt cggcgtcggc accagcaatg
     4021 gcatgccaat agatgtgact ggtcttagca cacccgatat gaagcaggaa agcaactttg
     4081 aaggttttga cttcgataat gtgtggtaca ttgatgaagg ggtgacctat ccgttggttg
     4141 gtgttctggc accataaatt aaatttcttt aacctttatt ccaccatttc ctctcctgca
     4201 aatcgggaga ggaaatggtg ctttttccgc ccggcagata ttgtataaat ccctattgag
     4261 ttaacggagt tgaacatgtt tcctataaac aggaagaatc ttttcgcgtc cgtcattata
     4321 tttttctttt ttatagccct attcgcgctg ccggctgccg ctcaggaaag ggcgggcgag
     4381 atcatccagc agttcgagaa aaaggaggtc gttccccgcg atctgccgga catgccggtt
     4441 attgaacaaa aagaggaaaa gacgcaggag gcgccggccg gcccgaaaat tctgatcaaa
     4501 caaatcaacg taaaaatgca cacaaaaacc ggaggggaag aaaaagcgct tctgcttcca
     4561 cccgacacca ttaaaactat tacctcccga tacgaaaaca gggaactgac ttttgcggaa
     4621 atcaatcaga ttgcgaatga catcacggcc gcttaccgca gccggggtta tcttctggcg
     4681 tacgctttta tcccgcagca ggaagtgaag gacgggctgc tggaaatcgg agtcatcgaa
     4741 gcgcaactgg gaaaaatcac cgtcgctggc aacacatcct acagcaccga tttcatccgg
     4801 aggcatctgg aaaaaatccg caaagatccc tccatcaaaa cagacaccct gcagaactca
     4861 ttgctgctgc tgaacgaata tccctccctg aacgtcatag ccgtcatgga ggctggaaaa
     4921 gaattcggca caacggacat tgtcgcgcag gttcaagaca gccggcccgt gtcgggaagt
     4981 atttcctatg ataatttcgg ctcgaagtac accagtgaaa accgtctcaa tgccgatctg
     5041 aacatcggca acctgctcgg cagcggggac ctgctgacgc tcagaggcat aaccggactg
     5101 gacgagattg attttgacaa tctgtcctac ctccgggcgg aatatgccat tccaatcatg
     5161 tattacggaa caaagatggg ggcttactat gccgacagcc gatacgaagt gggcgaggag
     5221 tacgaaccgc tcgaaattca cgggaaaacg catctggcgg gcctttatct gacacatccg
     5281 ttgctcaaga cccgactgca cgctctggat ttgaaactgg gttttgatta caaagacatt
     5341 tacgaataca tgcttgataa actggccagc aaggataaga ttcgcgtcgc cagcctggga
     5401 gcaacctaca acttcatcga taaattccag ggaagaaata ttatcaacct gacgtaccat
     5461 cagggcatcc gggatctgtt cggaggcacc ggcaagaacg aacgggaatc caagcggcag
     5521 ggattgtggg aacaactgtt cggcagcagc gaaattaatg taacgagaac ccccacgagc
     5581 cggctttacg ccgacggcgg tttttccaag ggtacgctgg atctcgccag aatccagaaa
     5641 cttcccggat ataattacat gatcctgaaa gggtccggtc agatttccag cgataatctg
     5701 tttgtggccg aacaattttt catcggcggc atcagcacgg tcagagggtt ttcatcggca
     5761 tcgcagggcg gcgatcaggg atactgtgtt tcagccgaat tccatttgtc gcccgttttt
     5821 cccgaggcta aagttttcaa tcagaccttc ggcgacatgt tcaagtttgt tttatttgcg
     5881 gatcacggcg gtgtgtttaa aaacgacgtt ctgccgggag aagacgagga cgactatctg
     5941 accagcatcg gagcagggct gagactatac atgggtaaac attttttcgc ccgcctggat
     6001 tacgccgtac ccagaataga cggctccttt gaaaccggcg acgcggaaat ctacggacag
     6061 gtggcatttg ttttttaaaa cataatggca ttctccgtct ggtctgataa aaaacatccg
     6121 cattttgaat gtttttgaaa tttttataaa cgcgttttta cggcgccgca ggagaggggc
     6181 gaacctttcc atgaaatatt cctctccaat ccaacccgaa cagattgatg atgccccgcc
     6241 ggaccatcaa gtttcaaatc aagtcgtttg caaaaacaac atattgatat ataatacata
     6301 ccggttgcct cttaaaggca aaaaagcaat gaccggtttg tctgttttgt gacggagacg
     6361 tcattcatga aacatcgaat atatttcaaa gccctgacgt tcacaggatg cctcaatctg
     6421 attctgccgc tgatgctcct gttgattctg tcggcgtgcg gcatggcttc atcctctcag
     6481 cgcgtcggcg gaccgtgcac ttacaaacag tatcaaggtg acgctgaaat cgtctcggtg
     6541 acacccgtaa agggaaaggc agaggagtac gaaatccggt tttcctttca tccgcgggaa
     6601 aagattcagg aagagtttgc ccgcgtggaa gggaaacagt ggctgctggt ccgggaagat
     6661 ttttcttttc cgaaaaaaga ttttctgcaa caatacggta tcaggaaggg aagtcgtttc
     6721 ccttgcgtaa tgaacgtaat taccagagga acttgcaccc cgattctgtt tgattttccc
     6781 acaatacgca acggcagggc gcaataatcg tgttaagact tttaatccca tgcctgaata
     6841 acacaggcaa gaccggaggt cgctgacatg aaacaaaaat tcctatggcc caacattact
     6901 gtttttatca tattaatatt tttcatatta ccggtatccg gccaggcgga cagcaccacc
     6961 tcggggcaac gagagcgtta cgttctcgat accaaccgtc ccgtaaagac atttcccatc
     7021 atgcgtccgg acgatctaac gatcagaaaa tggcagagca gatttgaacg ggcgccgcgc
     7081 gcgcacatcc gtgaaaaata tcaccgccgg gcccatcacc gggcaaaagc gcgcacagac
     7141 cagacaggca aggtatcctc catcaatctt cttgggcttt tgacgtatac accttcggaa
     7201 aaccagggat cgtgcggcga ttgctgggca tgggccggaa cccgtgttat gggcattgat
     7261 ctgaatacgc aacaaggctc tgccgacaat ctttcggttc aatttatcaa ttcctgttac
     7321 ggggaaaacg cctgttgcgg cggctggctt tccgacgttg tggatttcta caacgggaaa
     7381 ttgatggcca tttccgcagc caatacgaat gcgtcctttc aggacagcgc cttccggtgc
     7441 agttactttg gtgctttctc cagggtatcc tgcgaaaata ttacaacaga ggcaaactac
     7501 cccatccaat ccatctctga agccacggtg cacacgaccg gtatttcaca ggccgaggcc
     7561 atttccaata ttaagaacgt gctgaatcag gggaaagcca tctggttcgc gtttttcctt
     7621 cctgacaatg cctcttggaa tagtttttat aatttctgga acaatcagtc ggaaaacgcc
     7681 ctctgggatt cggacaattt ctgcggtcaa acctgggaca cctatgaggg cggcggccat
     7741 gcggtcactc tggtcggcta tgatgattcc gccgccgata cggatgatca ttactggatt
     7801 gctctcaaca gttggggaac aaccgccggc agacccaacg gtatcttcca cgttaaaatg
     7861 tatattaatt acaattgcac gcttcgcggt ctttccagct atccgcttgg atttcagacg
     7921 ctgaacattg aatggggtgt cgcacaggct gatcccgttg acagaacagc gcccgtcgtg
     7981 acagccttct cggtccccac ggtttcaaaa tcgcttacgg tgcctgtcaa cgtctttact
     8041 gcaacggaca acatggaggt aaccggttac atgattacgg cttcttcagc gaaaccgtca
     8101 tcatcggctg ccggctggtc ggcaaacgct ccgacacgtt atacattcca gtcggggaca
     8161 ctgggcacaa aaacattata tgcctgggcc aaagatgccg cgggaaatgt ttcatcttac
     8221 aaaagcgcct cattaacgct gagtatgcct gatctgatcg tctcggccgt gagcaactgg
     8281 tctacaacca tcactgcatc ccggagaagt ttttccatta ccgacaccaa taaaaatcag
     8341 ggcaatgctg cagcgcccgt ttcagtaaca aaatactatc tatccacgac cccctccagg
     8401 acggccaatg ccatacttct cggcggaacg cgctccgtta aggcaattgc agtaggtaaa
     8461 acgtcgagag gatcagcaaa ggttaccgtt ccccagggga tagcgaaaac ggtctattat
     8521 gccattgcct gcgcggatga taatttggtc gtgaacgaaa gcagcgaatc caataattgc
     8581 aaggcgtcat cccggaaaat taccgttaaa tactgaggat aagacgatat tccagcaatc
     8641 gctgattttg ctgatcaaaa ccgaagatta ttcaaaaagg gggttatcct gacaatacgc
     8701 ttctgtttca cgaaaatgac ggtaaggaat cggttttgcc ggcttatggc ggcatggtag
     8761 aaatacccgt gtttgcagtg atttgccgtt caaagaccct ttcaatccgc tgcccagcac
     8821 acctcaataa gagtaaattg catcttgtaa ttaattacaa ttatggtaat gtgacgcgcg
     8881 tactttttca ctcaaaaagt gatcgtaaaa aaactgcaaa atactgaaaa gtatttttat
     8941 ctgaaaagag gacttatgaa gaacacattt ctattcattt ggatcattgt aatgctcgtt
     9001 gcattttcct tcccgtcgat cagcttttcc gcgacgcagg aggttgtcgg ccaatattgc
     9061 gacgtgtata ccggcgatat gaaaaataag accgccttgg acgatttcaa aaaatctgta
     9121 aatgaacatg cccgaaaaga cggtttcaaa aaaattgtca aagcatcgcg tttaaccaat
     9181 gtgacgtctg actgcatcaa ttatgctgtc agtcattacg tggacaaggt caccgtcctc
     9241 agtcacaccg aaaagacgga aaacaaagga agacgaatat gtgaaaaggt gaaaatgacc
     9301 tatgattcag aatccgtcag caagtatctc agtcaggaat tttgtttaca gggctggaat
     9361 gcgaacgagg aaaaggttct ctggtgttat gacgtcgaca cggctcttga gagtaaagaa
     9421 ggaaagctca atgtcggggt gatcattgaa gcccagatgg ccaatatcga tcccggcaaa
     9481 aaggaccttt tggaacagga agaagaaaag cagtttctgg aaatgacgca aatcaacaag
     9541 ggcaaatacc aggttctcga caggaatgct tttatgaagg ctgccgagca acagaaaatg
     9601 tcattatacg gaataacgga taatgatatt ttaaagttgg gaaaactttt gaatcttgat
     9661 gttgtggtgc accggatcat taatgacaac gacagaacaa cgaaagtcag aaaaatctct
     9721 acgggaaaag ttcttctagc caaaacgtat gtaacagaga gcaaagcacc cgccgttgca
     9781 cccgattgga tcaaatatgg caaggaaaca aacggcaccg ttcatttcta taagaagggg
     9841 gaggccgtca aagacagtga cgaaagcatt atcaaggtat tgaataaatg ggtgttctca
     9901 aaaagagagg tgtccaatgc cattcaatac cggcgtgaaa acaggttgtc gaccaaaggg
     9961 tatgacaaat tatcgcacat gatttattta tcggaaatcg attgtcaaag taagagcgaa
    10021 agaatgatct acctgtattt atacgaccag gacgggaaag cattgtattc ccatagctac
    10081 aaaaccccgg aatggaacac gatacaactt tattcaaacg gagaagctct tttcaaagca
    10141 gtgtgtagat aatctttcgg aaaaaggtgg acgtttttcc gctcagcaga aacaaagacc
    10201 gtgccgaaac gctcatcaac agcgaagtgg cggccatccc gtgatgtcat gaaaccacct
    10261 gaaactatcc tgttgatcat cagaagatcc cttggcgatg tcctggccgt ctctccggtg
    10321 gtcgatatcc ttgcacgccg attcgcctcg gcctccatcg acctgctggt caaccaagac
    10381 acggcggcgg cggcaaacct gttgaagggc atccggaaca tcattgcgtt tgatgacggc
    10441 gcgctgaaga caacccctgt gtcctctttc atgatgcaaa tcggattatt gcaaaaaata
    10501 ttccgacgtt atgatctagc cattgccttc acgacaaacg aacgaagtaa tctatacgca
    10561 tttttcgccg ggcggacatc gatcggcgca agggagaagg tttttgcaaa aaactggtgg
    10621 caccgtttgc ttcttgacgg cggctatctg atcgatccgc aacgtcatat tctggaaaat
    10681 aatctttccg ccctgagcac cctcggcatc tctatcgaca gaatcgtgtt gtccgccaca
    10741 tacgccgggg cgtccggaca aaaagtgcag cagatgttga aagatttcgg catggaccgt
    10801 ttcatcattt ttcatccgtc ggggcgttac cggtgcaaat cctatcctga acaacagaga
    10861 aacagccttt tgcggttgct tgactcgttg aatattccga ttatcgtcac aggcggacac
    10921 agcgacatcg accggcaggt tgccgccacg ttaccggaat tgaaaaatgt gcggtcattc
    10981 atcggcagac tgtcgcttga ggaatgtgcc gctttaaccg acctggcgct ggcttatgtc
    11041 ggggtcgaca ccttcaacgc tcatcttgcc gcagccttca acaaaccggt ttttgtcatg
    11101 ttcggcccga cgattccgga aatctggtcg ccctggtcaa acgatctgca gacagcgggg
    11161 cgccggaatg catcgatgca gatttatgga aacatcactc tttttcaatc cggcatggcg
    11221 tgtgttccct gcggacactc caaatgcaga gagacggacc ggccatcgcc gtgcatggag
    11281 gccatcgatc cccgcaatgt tttccatcag gtccaacaat ggtttttaaa tttgtctccg
    11341 gaaagtcagg acaatgcaaa aaatgtttta ccggtttaaa aatttttcta cgtgcgttgt
    11401 gtgcgatcgg tcttttctat cggacgggat gagggcgctc ctggacgatc cggagaaata
    11461 tctgcagcag gccgaagcca gaaccatcca agacaacttc aaaaacaaaa tcgtttattt
    11521 tgagatcgac aagaaaaaag ccgtcattaa aattcataaa tacaaatccg cctggcacag
    11581 gatcaaacgc tatttcaggc aaacgcgggc gaacagatca tgggcatact ctttgctgtt
    11641 caacgcgcac gggataccta cgccgcaacc gattgcctat aaagaatcca gaatcgggcc
    11701 tttgcgcggc gattcctatt tcatctatga atgggtcgaa ggcatcaacg gtgaagaata
    11761 tttcacgaaa aacaaggcca atccggaaat tgtcgaaaaa gccatagacg ccatcgtgga
    11821 catgacccga acgattaaaa aactcggtct catccacgga gacattcgtt taaaaaatat
    11881 ggttttcaga ggagacgacc ttctgttaac cgattttgat gatacgaaaa agaggcagtg
    11941 gtacaagagc aattacatga ataatcgcga tttcagaggg ctgattgaag acatttatta
    12001 caatgtgcct ccggaaattc aaccgctttt tttatcccgc ctggaatctg tcggcgacga
    12061 tatccgaaaa cttgtccggt catacaaaca tcgtccttaa gggccgagtc cgcaagagag
    12121 ccaatcttct cttgacttgt ttccatggct tccctacaat ctttttcgga tgaagcatat
    12181 tgatcagatt tcattccaag aaaatcagat tgaaaaaatc ataaggaaca acacgtgacc
    12241 gcaaccatag cacccgcaaa acttccggtg atcctcttgt ataatatcga tccgggttgg
    12301 acgccggaag agaaagatga agcaatcaaa caatcggccg aactgggaga tgccctccgc
    12361 tgcacaggcc acccgacgac cctggttacc gtgactgata acgacattgc cgagcagctt
    12421 cgtcccttcg atccggcaaa atacatcgtg ttcaactggt gcgaggaact tccgggcatc
    12481 ccgcacagcg aatggaaagt ggccaaacaa ctggagtcgc tgggttatac gtttaccggt
    12541 gccgattcgg ccgcgctgtt gctggcgcag gacaaacagc gggtgaaggc cctgctggat
    12601 cagtgcgcca ttcccacgcc tgcatggaaa atcttcgatg cgcctgaccc tcagggctgg
    12661 aaaatttttc cggcaatcgt caaacctcaa aacgaacatt gttccgcagg catcacgccc
    12721 gagtctgttg ttctgcaacc gacggatttg caaaaacgca tcacctttgt gctcgatcat
    12781 tatgcgcaac cggcgctggt ggaagacttt attgacggac gggagtttca tgttgccgta
    12841 tggggaaacg aaaccgtcaa cgtcctgccc gccgcagaga tggacttttc ccgcttcgga
    12901 gacctcaatg accggctctg cacctacgag gcaaaattta ctcccgggtc gctgcattat
    12961 gaagagatca aaacccttct gcccgcccca ctgaccacgg gtgaaaaaaa gtcgctggaa
    13021 acggtttgcc gcaacgccta tcgggcatcc ggatgccggg attatgcccg catggatata
    13081 cgcttgcggg acaatatttt ttacgttctg gatgtcaatc ccaatgcaga tatcagttcc
    13141 gatgccagca tggcctgcgc tgcggaagcg gatggaatca gttatgccga aatgggcagc
    13201 cgcatcgttg agatggccgc aaaaaggcat ccggtccttg ggaaactctt ccggagttga
    13261 cttttaacag gcgatggttt agacatacgc atattcatta agggagtcgc cttgtcatga
    13321 acaaaaaaga ttttctcaaa accaagatcg aacacatcga tattaaaaaa ataaacgcgg
    13381 tggacatcgt cagcgcgatg aaaaaaatgt ccttttccgc ccgcgatctg gccaatgccg
    13441 ccgatatata cgaccgtatg ttgaaagaaa aaaactgcgc ggtgatcctg acactggccg
    13501 gttcgacatc cgccggcggc tgcatgcagg tttatgtcga tttggtgaaa aacaatatgg
    13561 tggatgccat tgtggccacc ggcgcatcca ttgtcgacat ggattttttt gaagcactgg
    13621 gattcagaca ttatcagggc acaccgtttg tggacgacaa actgttgcgc aagctctaca
    13681 tcgaccggat ttacgacacg tttattagcg aaaacgattt gcaggcctgc gacaagacca
    13741 ttaaaacaat cgcggatggc cttgcgccgc ggccttattc ttcccgcgaa ttcattgccg
    13801 agatgggcag gtacctcaca aaaaacgcca aaaagaacaa ttctctggtc caggcggctt
    13861 atgaaaacaa cgtgccggtc ttctgcccgg cattttcgga cagttccgcc ggtttcggcc
    13921 tggtcctgca tcagcatgaa catccgggaa accatatgtc gattgattcc gtcagggact
    13981 tccgggaact gaccgatatt aaaatcaaag cgggaacaac cggcttattg atgatcggcg
    14041 gcggtgtccc gaagaacttt gctcaggata ccgtggtttg cgcggaaata ctgggctacg
    14101 atgtccccat gcacaaatat gccattcaga ttaccgtggc ggatgtgcgc gatggtgcct
    14161 gttcaagctc aacgctcaag gaagccaatt catggggcaa ggtcgattcg tcgtgcgaac
    14221 agatggttta cgccgaagcg acaatggctc tgcccttgct ggccagttat gcgtaccaca
    14281 aaggcaactg gaagaaacgg aaaaaatgga atttatccag gattttcaaa taaatatctg
    14341 cctgctgtat tcaatagttt tttaatgcat tggtaacttt tacgggcgga ataaccgtca
    14401 ttatggggaa aataaccgtt ttttctcaaa acgatttagg gccccgtacg gtcagacagt
    14461 ctccgttaag ccattagtta ttaattttat taaatattat cttaaaactt gccgcgctgc
    14521 cctatcactc atttaaggcg acattgggca cataaattgc ttaaagtcat agaatatgca
    14581 tcagcttttc ttcatgaata cgcgattggc gtgatcaatt gcattggtca gctcatcacc
    14641 gaaaccggca acgtaacttg aataacgatg gaagacaaaa acgaacgaac aaaattaaca
    14701 gaaagattgt tttcttctga agacaacaat cttccattac tggcaaaaga tattatatcg
    14761 aatgccggcg tagcaattta tatcgttcac aacagtcaat ttgtttacgt cagcgaatta
    14821 taccagaaac tcaccggcta cgcggaaaaa gaacttatcg gcgaatattc cctcaaaaat
    14881 attcatcctg atgacaggga taaagtcagg aaacaggcca ttaaatgcct gaaaaaagaa
    14941 agttttgagc cctacgaata cagatatatc cgcaaagata atgaaatcag atacattctg
    15001 gaaacagtgg cgccgattat ccataaaggc gagcgggcga cactgggcag ttttatggat
    15061 atcacggaac gcaagatcat cgaagaaaaa tacagaaaca ttctggaaaa tattcaggaa
    15121 ggttattttg aagttgatct tgaggggaat tttacttttt ttaataatgc cgtctcccgt
    15181 gttcttggat attcccggga agaattgatg ggcatgaata accggcagta cacacccgac
    15241 aaggactccg cgcgaaaagt ttttcaggct tatcatcatg tttacgaaac aggtgagccg
    15301 ctgaatgcag tcggctggca catcattacc aagcaaggcg ccaaaagata tattgaagga
    15361 tccatttcgt tactcaagga ttctcaaggc aaaccgatcg gatttcgcgg tatcacccac
    15421 gatgtcaccg aacgtaaagt ggccgaagaa gcgctgcgtc aaagcgaaga aaagtacaga
    15481 aacattctgg aaaacattca ggaggcctat tttgaagttg atcttgcagg caattttatc
    15541 ttctttaacg attcgctgtg tcgcatcaca ggaggaacaa gagaagaatt aatgggtgcc
    15601 aattacacgc ggttttccga tccggcaacg tcaaaaaatg tgtttctggc attcaataaa
    15661 attttcaaaa caggagaggc cacggaggga ttcgactggc tgattatcag aaaagatggt
    15721 tccaaaaaat atattgaagc gtccgcatcc ttaaataaag atgcttcggg aaagcccata
    15781 gggttccggg gagtcatccg cgatattacc gaacgcaagc gcattgaaca ggaactcaat
    15841 cacatggcca cgcacgacgc gctcaccggc ctgccgaacc gcctgatgtt cagccagttg
    15901 ctggatcacg ccatccaatc cgcgcaacgc aacaaaaaac acctcgctgt tttcttcatc
    15961 gatctggatc gcttcaaaat cattaatgat tccctcggac acgaagccgg agacactctg
    16021 ctcaaagaga tggctttgcg gttcaaacag tctctgcgta ccgtcgatat tatcggacgt
    16081 ctcggaggcg acgaattcac gattctggtt gaagatttta acgatctgaa gcaggtggaa
    16141 tttctggcca agaagatttt gaacaccgtc atgcaaccga ttgcgctcat gggggaagaa
    16201 tgtcgtgtca ccgccagtat cggtatcagc gtcttcccga aggacggact ggatgaacag
    16261 tccttaatga aaaatgcgga cattgccatg tactttgcca aatcggaagg aaaaaacaat
    16321 ttccagtttt actccgaaaa tatccgctcc atgtccaacg aacggctgac catcgaaacc
    16381 aacctgcgtc atgcaatgga acgcaatgaa ttttacctcg attaccaggc aaaactggat
    16441 ctgcagacca gggcaatcac cggcgtcgag gcgcttctgc gctggaataa tccgtatctg
    16501 ggactggtcg cgccgacgca gtttattccg attgcggagg agacaggcat gattataccc
    16561 atcggccgat gggtgatcaa aaccgcctgc atgcagaacg tcgcctggca gaagcaggga
    16621 ctgccgcccg tctgcatgtc ggtcaacctg tcgcttcgtc aactcatgta tgaaaacttt
    16681 gcagaagatg tcaaaaccat tctgaatgaa acgcgcatgg cttcggaact actggagctg
    16741 gaaatcaccg aaagcacggt catgcacaac cccaaacgtc ttgtcccgat cctgtcaaaa
    16801 ttaaagaaaa tgggaatacg gatggcaatt gatgatttcg gcacgggtta ctcttcgctt
    16861 gcacagatca aaaacttccc gatcaacacg ctgaaagtag accgatcatt tatccgcaat
    16921 cttccgcagg ataccgataa taaagccatc acggaagcgg tgatttttat gggcaaatcg
    16981 ttgaaactca aagttgtcgc cgaaggcgtg gaaaccaggg aacaggcgaa atttcttaaa
    17041 gatcagatct gcgacgaaat gcagggcttc tatttcagca aacccatcgg gcctgacggc
    17101 tttgccgatc ttcttcgcga aaattctgtt tcgggaacag atcccggccg tcaataattt
    17161 ttttgtaaaa aacaaccggc attattgtgt catctgcaaa aatcagtttt tcgccagaca
    17221 ttcttccagc ggggaaatat ccacggatcc cttgtcgttg acattgtttt tgaattgtct
    17281 gacgccttga gcgaccgaat gaagattcga aggtgaggtg actgcacagg cgttcatttc
    17341 cgagagtttt tcgatatcgg atgccagaaa atcaaaaatt ccggtcaccg ccgcctccac
    17401 aatatcggac gtgacgagat ttgcgcgtcc ttctccgtac agatcaaacg caaagctttt
    17461 tatggccatg ctgtcgataa tctgagttac ggtatatgcg acgtcataaa ttctatcata
    17521 cgtgatatcg ggaacacgac caagtccgaa taataacagg atttccgaac cgatcctttc
    17581 cggaaaagga ataacaactt tttccttaaa accgccgccg attcttccat ttttgatttc
    17641 ccgggaaagc atgccattga gacgccagtc gatcaaaccg ccgatgccgc gcggcggctt
    17701 ttcgtcttca aagatgccga gcgccaaaca tttatgtttt ggaagatcgg ggttttctat
    17761 cgagagactt agctgcattt tttttattta atttcagatt gagagcgtcg agtatgccgt
    17821 tgatgaacga tccggagttt tccgacccga agatttttcc cagatcaatg gcctcattca
    17881 tcgtgacctt cggaggaata tcatcacagt agagcaattc aaatacggca agacgtaata
    17941 tgttgatatc cacgcgggac attcgcccca acgaccagtt atccgaacat ccggcaatga
    18001 gttgatccag ttctttcctg tggtcgcatg ttcccatcac gagaaaggcg gcaaaatcct
    18061 tggcagattt tggtgcaaca aaattacccc agaaaagatc cagggcttcc tgcgccggaa
    18121 tatcgacaaa attaagactg taaagaacct gtaaggcaat ttctcttgcc tttcgccgcc
    18181 cgcacatcaa ttttcaacct caaagaacca gaacatccga tcatttctgt actgttgccg
    18241 tcagaaaaaa cggcattgca acatcaaatt tttttgaaca aatccaccat ctcaatggct
    18301 gtcatcgctg cgtcggcccc tttattgccc gacttcgttc ccgcacgttc gatagcctgt
    18361 tcaatggtgt ccgttgtcaa cacaccgaaa acaacgggca cttccgtttc aaggccgaca
    18421 acggcaatcc ccttggagac ttcggcactg atatattcga aatgcggcgt tgcaccgcgg
    18481 ataatggccc ccagacagat gacggcatca aatcgaccgc tcttggccag tttctttgcc
    18541 gtcagcggca gttcaaaaga gccgggaact ttataaatga cgatatcctt ttcttctgca
    18601 ccggccctga tcagcgcgtc gatagtccct tcgattaatc tgccgcatat aaaatcattg
    18661 aaacggctgg ccacgatgcc aaacttcatt ccctttgcaa cggtctttcc ttcaattatt
    18721 ttcagcatag ttcttaacca tccttacgtt ttattttagc cttgcggtgt aaaatctata
    18781 ttttcaaaag gtgccccatc ttttttttct ttgttttcat gtagtgaatg ttgttttcat
    18841 tgggatcgat ttcgatggga acgcgtttga ccacttcgat accgtatcct tccagaccga
    18901 caatcttctt cggattgttt gtcaggagac gcattttgcg cacgcccaga tcagccaaaa
    18961 tctgagcacc gatgccataa tcgcgtaaat ccggtttgaa tccgagttcg atattggcct
    19021 ccacggtatc cttgccctgt tcctgcagag cataagcttt gatcttgttg accagtccga
    19081 tgccgcggcc ttcctggcgc atataaacga taatgccctt gccttctttt tccaccatgt
    19141 tcatcgcacg atgaagctgg tcgccgcagt cgcagcgcaa cgacccgaaa agatctccgg
    19201 tcaggcattc ggaatgaacc cggaccagga cttcgtcatc ttctttaata tcgcctttca
    19261 ccagggcgat atgctgccag tcgtcaactt cattttcata aacgatcatt ttaaaatcgc
    19321 cgccgaaact ggtcggcaag gtcgcttccg ccatacgttt gataagcgat tcattctgca
    19381 tccggtaatc gataaggtcg gcaattgtca ctatttttaa cttgtgttcc ttggcaaata
    19441 tttccaaatc cggcatccta gccatggtgc cgtcatcctt cataacttca cagatcacgc
    19501 cggccggtgt caaaccggca agtcggcata aatccaccga cccttccgtc tgccccgtcc
    19561 ggaccagaac cccccctttt ttggcacgga taggaaagac atgtccggga ctgaccaaat
    19621 catccggttt ggcctccgga ttcacagccg cctgaatcgt tgttgcccga tccgctgcgg
    19681 atataccggt ggtgacgcca tggcgcgctt cgatggaaat ggtaaaagcg gtgccgaaac
    19741 ggcactgatt atcctgaacc atttgtttca gatgaagctt atccgccata tcttcattaa
    19801 gggtcagaca aatcaaaccg cggccgtagc gggccataaa gttgatggtt ttagccgtgg
    19861 caaattgcgc tgccatgcaa agatcccctt cgttttcacg atcttcatca tcaacaagaa
    19921 tgatcatttt cccgtttttg atatcttcta ttgcttcctg tattgtgctg attgccattc
    19981 gtctttcctt tattccttaa aaaccgtgtt tcacaaggaa atccttatcg atccccttat
    20041 ttgattgcaa taagttttcc acgtaccggc ctaaaatatc cgtttcaata ttcacccact
    20101 ccccttcttt gcgggttacc agggtggtat tgaccgccgt gtgcgggata atattaacat
    20161 aaaaccggct tttttcaagt ttgttcaccg ttagactgat gccgtcaatc gccaccgaac
    20221 ctttttccac aatgtagcgg gaaatcgtat cattcatctc gacagcgata atcagtgaac
    20281 ccgatttttg catccgggac aggattcttg ccgtggcatc gacatgcccc agaacaaaat
    20341 gtccgccgac aaaaccgcca acacgcagcg atttttccag attcacctta tgccccgcct
    20401 gtaacaattt aagcgtcgtt cgggacagcg tctccgcgga aacatccgcc tgaaaattat
    20461 tttgctgcag ggaggtgacg gtgaggcagg ctccgttcac ggcaatactg tcgccaagcg
    20521 aaacgtcttc cagactgatg ccggcgtcaa tgtccagaac cgcatctgcg cccttcatgg
    20581 tcaaacgcct caccttgccc agtccttcta cgattccggt aaacatgatt actttttacc
    20641 tttgcttaaa agatccttca gctccgacat gaactcaccg acatcgcgaa actgccggta
    20701 aacggacgca aatcggacat aggcgacgcc gtcgaaattt ttcaactcgc tcatgatctt
    20761 ttctccaatg accgtggacg aaatttcttc ggcctgcatt tcctgacagg cctgttcaat
    20821 gttttcgaca agtttgtcaa tcgcctccat gctgatcggt cgtttctcgc aggctttttt
    20881 gatgccggtc agaattttct gccggtcaaa ctgctcgcgg cgaccgtctt tcttcaccac
    20941 catgggcaga acgtcttcca cgtattcata ggtagtgaaa cgcttggtgc accccaggca
    21001 ttcccggcgc cggcgaacgg ctttcccgtc cttgctgatt cgagaatcaa tcaccttatt
    21061 ttccgtgtga ccgcaaaaag ggcatttcat gatgatgtca ccttatgtgg ttaaagttgc
    21121 tctacgcgga tccccgcttc tttgagcatc tccgcagcca gttgatcatg ataaccgtca
    21181 cggatgacga cacggatgat cccggcatta atgatcattt tggcacaaat gacacagggg
    21241 tgattcgtgc aatacagcgt cgatcctttc gtgctgacgc cgtgcagcgc cgcctgaata
    21301 atcgcattct gctccgcatg cagcccgcgg cataattcgt gacgttcgcc ggaaggaacc
    21361 ttcaactgtt cccgaagaca gccgatatcg agacagtgct gaagcttgga gggagcgccg
    21421 ttgtatccgg tggccagaat tcggctgtcc cgaaccagtc cggcccctac cgatctccgg
    21481 caacaggtac tgcgcctgga aacaaggtcg acaatatcca gaaaataact gtcccagtcc
    21541 ggtcgttttt ttttcttctc ccgcaaaacg gctgctgacg attttatttc catcataatc
    21601 gctttcgatc aaaaaaaatg gtccgtgttt taatttttca gccgcccctg atacagcgga
    21661 aaacgatcgc aaaggacttt gatcttctgt gcaaccgatg tgattttctg ctcgctactg
    21721 atgtttttaa tcacgtcagc aatacacgac gcaattaaac gcatttcatc ttccttcatg
    21781 cctcttgtgg tcacggcggg cgtgccgatt ctgataccgc tggtcacctg aggccccttc
    21841 gtatcaaagg gaatgccgtt cttattgacg gtgatggagg cgcgatccag agcgtcctgc
    21901 acgtcctttc cggtgacatt cttgtcggtc aagtcaacca gcatgagatg attgtcggtg
    21961 ccgccggaca ccagccggaa tccctgattc ctcagttcat ccgccatcgc ctgagcgttt
    22021 ttaatgatct gtgcctgata ttccttgaat gccggctgaa gcgcttcctt gaaggcaacc
    22081 gccttggcgg caataacatg catcaacggg ccgccctgca tgcccgggaa aactctgctg
    22141 ttaagtgttt ttgcgtaagc ttccttgcac atgaccacac cgccgcgcgg tccgcgcaac
    22201 gttttatgcg ttgttgtagt gacaaattcg caaaccggaa ccggagaggg atgcaatccg
    22261 gtgcaaacca gaccggcgat atgcgcaata tccgccataa tcacggcgcc gacttcatcg
    22321 gcgattttgc ggaatttatc aaaatcgatg gttcgcgggt aagcactggc gccgacgata
    22381 atcattttgg gcttgtgttc cttggccagt ttttccactt cgtcaaaatc aatggtttct
    22441 gtttcccggt tcacaccgta ggggacaatc ttataaaact tgccggaaaa gttggccgga
    22501 ctgccgtgag aaagatgccc gccgtgagac aggttcattc ccagaatggt gtcgcccggc
    22561 tgtgcgacgg caaaatacac cgccatgttc gcctgtgttc ccgaatgcgg ctgaacattc
    22621 acatgatcgg ctccgaaaag ttttttgcag cgctcaatcg ccagactttc cgcaatatcc
    22681 acgtattcac agccgccgta gtaacgtttt tccggatacc cttccgcgta tttgttggtc
    22741 atcacggagc cctgcgcttc cagcaccgct tcactgacaa aattttcgga ggcaatcaat
    22801 tcgatttttc ccgcctgacg gcgtgtttcc agatcaatgg ccttcattac ttccggatca
    22861 actttttcta aaaaagacat gaaaattttt ctctccttcg ttctaaattc tgcttattgc
    22921 accatccccg tttcgcttca caccagggat aatttccaat cccgatatat cggaatggga
    22981 tcattcgtcc aacaaatttc agcgagctac gctttatgaa attgggactc attaaatttc
    23041 ttaaataaca aaaccgcatt gacgccgccg aaaccgaacg tattggacat ggccgtatgg
    23101 attgcctgac gacacgccgt gttgggtaca taattcagat cacacccttc accgggattg
    23161 tcgaggttta tcgtcggcgg aataacgctt tcctgcaggg atttcactgc aaaaatcgct
    23221 tccacgccgc ccgcggcacc cagcatatga ccggtcatgg atttggtaga gctgaccaac
    23281 aatgttttcg cgtgatcccc aaatagagat ttaatcgcct gcgtttcgta taaatcatta
    23341 agaggcgttg atgttccgtg cgcattaata taatcgatgt cttcgggatt cagaccggca
    23401 tcctgtatcg caacccgcat acagcgggca gcaccttcat gaccgggtgg cggtgcagcc
    23461 atatgaaaag cgtcagaggt acaaccgtat ccggccagtt ccgcataaat tcgcgcccct
    23521 ctgttgagtg caaaggataa ctcctccagg atgacaatgc cgctgccttc gccgatgatg
    23581 aaaccgtcgc ggtctttgtc aaacggcctg ctggctttct gaggatcgtc gtttcttacc
    23641 gacagcgccc gcatggatcc gaaaccgccg acggccagtt gcgtcaccgc ggcttcaact
    23701 cctccggcaa tcatgacgtc ggcatcgctg taggcaatgg ttttgtaggc atcaccgatg
    23761 gccgatgttc ccgacgcaca ggctgtcaca gcacaattga tgggaccttt ggccccgaat
    23821 ctgatggaca cctggccgga tgcgagatta gcaagaatgg caggaacggc aaagggagaa
    23881 attttgcgcg gaccggaatc aaggagattt ttaaattctt cttccagagt cgccagcccc
    23941 ccgatggcgg aaccgatgat cacacccgtt cgttcggaaa tatcggaacc aatcgtcagg
    24001 cgggcatccg ccagcgccat ctcggaagcg gcgatggaat aaagaaggaa gttatcataa
    24061 cgacgggctt cctgtttgct gacatattgc agaggatcga aattttttat ttcaccggca
    24121 atttttgttt taaacgcggt gctgtcaaat tttgtgatca ggccgatgcc cgacttaccc
    24181 gcaatcgcag ccccccatga ttcatgtaat gaatttccca agggggtcaa cgcaccaaga
    24241 cctgttacga ccacacgtct cttcatgggt ttaagtcctg cacggctttt tattgaacgc
    24301 ctttctgtct cagaaaatca accacgtctt gaatcgtggc aattttttcc aactcctcgt
    24361 cagctatttc tataccaaaa gcctcttcca tttccataag gagctcaaca atatccagag
    24421 aatccgctcc caaatcgtca ataaaagatg cttccaaaac gcattcttcc tttgtgacgt
    24481 caagctgctc cacaataata ttaatgactt tttcttctaa cgtcatgcac catcctcctc
    24541 acgtgtatga ttgcttacca atttggatcc atgtgcttct tttatcatcg ccctccggga
    24601 atcattctgg ttccggttta aaagaagact atttgctcac caatggatta cagacacatt
    24661 ctgctctgtt tgaagttttt caaaatgact ttttagtcat ttttttccgc ttttctgtat
    24721 tctctgcctt tgtacgtttc gcaggtcgga caaatgcggt gaggcatctt gggttcattg
    24781 cactgtgggc atacggacat ggacggcgat ttcaaaaaat cgttcgccct tcgctggttt
    24841 cgtctggttt tggaatgtcg ctttactgga tttggcataa ttcacctttt tctataatta
    24901 gttttttatt ttaaaatttt ttaataccgc catacgggtg tccctgacat ctttttcgca
    24961 ctggcaggac gcggcattca aattggtgcc gcaacgctga cacagccctt tgcactcttc
    25021 gcggcaaaga attttaatcg gtatctgcaa aatgatttgt tcacagataa tgggcgccag
    25081 gtcaataaaa tctccctggt agctgctgat ttccaattct tccggtttaa gttctatctc
    25141 ctctttttgt tccgctttca ctggaatcaa ggtataggta aagttactgc cgatggtcag
    25201 cctggttctt tccagacaac ggctgcagtc ggcatcaatc agcgctgaaa agtttccctt
    25261 gatgaaaaca gtgcctgacg tttttgtgat catacaatcc acatcaatgg tttccaacgc
    25321 aaaatcaagg cgttcttcct tgtgaaagca cgttttaaac caggcatcgc cttcggaaaa
    25381 aacaaagcgc tgtccttctt ccggaatcag caaaacgtta attttcaaag aattagacat
    25441 agatagcccg ttgtctgaaa aatatcttaa atttaacctt gcgagtaaat tataaaatct
    25501 tttcattgtc aagtataatt tattgactcc ggttatgcaa taagcattta attttatatt
    25561 tgatttcaat tctaaacaat cgcgcggccg gatgaaatca taaatatttt catggtgctt
    25621 gacaaaagca tgctctcaac tctataaggc tataaaaaac aggacataaa catgcaaaaa
    25681 atcagaactc gcttcgcccc ttctcccacc ggttttttac atatcggcgg ggcaagaacc
    25741 gctcttttca actggcttta tgcccgccat cttggcggcc agtttattct gcgcattgaa
    25801 gatacggacc ggcagcgctc cacggaagaa tcaacaaaag caattctcga tgccatgtcc
    25861 tggctggggc tgaactggga cgaaggaccg cattttcagg cccaacgggt acaactgcac
    25921 cgggacatgg tgaaaaaatt aatcgatgaa ggcaaagcct attactgcac ctgttcgccg
    25981 gaagaactcg agattaagcg caaacaggcg ctggctaacg gtaagaaacc gaaatacgac
    26041 ggcacatgcc gtaatagaag cctggcgaaa tcggcaaaca gtgtggtgcg cttccgctcc
    26101 cgagacgacg gcgcaaccgt tgtggaagat atgatcaaag gaaacatcat ttttaataac
    26161 gaagagcttg atgatttgat tatcgagcgc agcgacggct atcccacgta caattttgcc
    26221 gttgtcattg atgatgccct gatgaacatc acccatgtca tccgcggaga tgatcatgtc
    26281 aacaacacgc cgcggcagat ccagctttat gaggctctcg gttttgatgt cccaaaattt
    26341 gcccacgtcc cgatgatact gggaagcgat aaaacgcgtc tgagcaaacg tcacggcgcc
    26401 acgtcggtca tggcctataa ggagctgggt ttcctgccgg aagcgttggt taattatctt
    26461 gtacgtctgg gttgggctca tggcgatcag gaaatttttt cgacggcgga gctcatcgaa
    26521 cgcttcagca tggacgccat cggcaagtcg ccggctgtct tcaaccccga gaaactgctc
    26581 tggctcaacg ctcattacat aaaagaagct tctgttgaaa gactgatgga agagatgaag
    26641 cccttgtggc cgtcggttat cgataccgga aacacggatt tcatcagaaa agtgattgcc
    26701 gattttcagc cgcgggtcaa aaccctgatg gaactggtcc agtcgtccga tttttacttc
    26761 gccgatgaaa ttcattatga caaagaagcg gcgcaaaaat ttctgacgcg ggacattgtc
    26821 gatcatatga aggcgattgc gaatacgatt tcatccgtgc aacagttcag caaggaaagt
    26881 ctggaagagt tttttaaaac atttactcag acccaaaata taaaattcaa attgatcgcc
    26941 cagccgctga gagtggcctt gacgggaaaa acggtcagtc cgggtattga cgaagtgatg
    27001 gtaacgttgg gtaaggaacg tgtcattaaa aaaattaata aagcgatagc gtatattgaa
    27061 tcgcttgcct gatccttatc gccgactcag agcggtccaa ttttaacttg acttttacag
    27121 gctgattcaa tagatagtca agcctcaacg aggcaggaat accgcgaaaa acgagcgttg
    27181 agtgcatccg ctcagaaaca taaaaacatg ccgccatggt cccatcgtct agtggttagg
    27241 acgctggcct ctcacgccgg aaaccggggt tcaagtcccc gtgggactac cagaataaaa
    27301 tcaagtattt ataaatattt ttaatttatt attcaatata tttattcact attcccaaca
    27361 ctattcccaa cactattatc aagtttaaat ttccccgata ttacctctga caacccatgt
    27421 ctcaagtatc ctataaccag cttatcaaaa aaagctaaga taatattata ataatacgga
    27481 ataacgtaag taataacgga ttaccgcttt attttatcaa ttaatctgaa attaattttt
    27541 tgataatata ataaaaaaag tcaatctatt aaataataat atagttatat tgaatatata
    27601 tctaaatatc tatcctgttt ttttctttga ccattaaaat ggcacattct tttctttaat
    27661 caatgacaac aaatttggag gaagctagga tgaatgaaaa aattttatat gtcactgaga
    27721 aggaggttag tcggatgacc tcaagggcat tatcgacatt gcgaaatgat aggttcctta
    27781 atcggggtat accttattac aagatcggcc gatcagttag atacttaaaa gcggacgtga
    27841 ttgattttat ggaggctggc aaaatcatga ccctatcaaa acaagaaaca aaacttgggc
    27901 aggaatcaac catgaagaac ggatgaccgg cgacagtgaa aaattttagc tgaaaatgga
    27961 ttattgggcg ctagtcgcca acaccagcag acataaaaca ggaggttatt ttatgtttaa
    28021 tttagatggg aaaaatattg acggcaaaca tcctataatt ttcgatttca tatattcacg
    28081 agattattca gatgggtatg ggtacttcaa gggagacaaa gaggcaatta gctatgcaca
    28141 agcagtcggc aaaaatcaaa aaatgaaatt taaaaaatat ggcattgtcc caaaagcatt
    28201 agtccagatg atggcacagt actggagttt tcaaggtaaa aaaaatccca gttaccgcaa
    28261 cgtgacctat ggcgagtttt tgacattact tattaatggt ccttataaaa gtgaggcagg
    28321 gaaacaggta ggggaaaaat tttatcggga tcttctcatt aaggatatgc catggattaa
    28381 acggtcattc gccatggctg ataaactcta tggcagataa tatttattaa tagggataaa
    28441 tttagaaaga agtgaggtga tgcttatgcg tgaataaaaa atgaagtgag ttgaaagcgg
    28501 cgaaaataaa ctggccggtt cctggcaaca gggatcggtt tttttatttc cgggaacctt
    28561 aaaccattta accaaaattc cataaggagg aatttgaaga tgacagataa aattttaaca
    28621 acaaaagaag gcaatgacct tcctaaaccg gcaagtgaag caacggaatc aaaagctgta
    28681 gtggtgcctc aaccgactgc gcccatggtg cctgcagaac ctgagaccga actctggtct
    28741 tatatgggga ctgacaacac gggtttcact gatattgcaa tagtcagtgt tgaagatgag
    28801 gatcgtcaat taattaaaac ctggatgggc aaaataaaaa aaacccaaaa gggccagaag
    28861 atatcgtatg aaaatgacat tcaagaaggt atttctttaa ttaaaagcat ttcctgcgag
    28921 gcgaataaac ttattaacat tgctaacagg aattttgctg acagggcaat ttcaataggc
    28981 atgatttgtc acaggctgaa gcaattgatt cgcggtgaca aaagaccatg ggccgtatgg
    29041 gcggaagaaa atctgccgtt tatagccaaa aggaatagag aaaaatatat gctttta
//
LOCUS       DAONQZ010000026        27208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1915_length_27208_cov_14.543513, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000026 DAONQZ010000000
VERSION     DAONQZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 27208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27208
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1915_length_27208_cov_14.543513"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /gene="ectA"
                     /locus_tag="PLR13_06080"
     CDS             <1..505
                     /gene="ectA"
                     /locus_tag="PLR13_06080"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HQC18939.1"
                     /translation="RLPEKKDAQAVRRLIEDSPPLDLNSLYCYLLLCTHFAPTSAIAE
                     YHQEVCGFVSAYIRPDQKDTLFVWQVAVQRDFRGKSIAKGMLLSCLQRAHLSSLRYLE
                     TTVNPSNSSSNALFSSLAEFLNAGLAKSALFSADDFGKTSHEEEILYRIGPFNISKEG
                     ESINENN"
     gene            492..>1189
                     /locus_tag="PLR13_06085"
     CDS             492..>1189
                     /locus_tag="PLR13_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQC18940.1"
                     /translation="MKTIRRLESEVRSYSRSFPVLFEKAKDAFLFDAGGTRYIDFFAG
                     AGTINYGHNNEYMKRKIFDYLENDGIVHGLDMATVAKEELLLKMEATLFKPKNLDYKV
                     QFTGPTGTNAVEAALKLARMVKKRSNIISFTNGYHGLTMGSMSVTANAYYRDEAFINR
                     SDVSFMPYDGYLGKHVNTAKYLRRFLEDNSSGVDIPSAIILETVQGEGGIRVAGDEWL
                     REVEKICRDFDILL"
     assembly_gap    1190..1289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1290..1585
                     /locus_tag="PLR13_06090"
     CDS             <1290..1585
                     /locus_tag="PLR13_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQC18941.1"
                     /translation="FRKGKILHKRLNKISAKYPQLHTEVRGRGFVYGMGLSTGEIAKK
                     ILTECFHNKLILESAGARNNVIKFLAPLTITDEVLEEGLDILERSIDNVMKKH"
     gene            1597..1986
                     /locus_tag="PLR13_06095"
     CDS             1597..1986
                     /locus_tag="PLR13_06095"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HQC18942.1"
                     /translation="MLVRTIDDIRGTRREVFAENKNWVSRRLLLKKDGMGFSFHETII
                     FAGTETHIWYKHHLEAVFCVEGEGEVETVADGKIHRITPGTLYALNDHDEHYLRAQKD
                     MRLLCVFNPPVTGEEVHLADGSYPAPE"
     gene            complement(2023..4740)
                     /locus_tag="PLR13_06100"
     CDS             complement(2023..4740)
                     /locus_tag="PLR13_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQC18943.1"
                     /translation="MESIICKHWHHCPSEEVTTLLNTDADKGLSPFEAKHRLEKYGPN
                     SVTMKKQKGPLMRFLLQFHQPLIYILMAAGAVTFFLQEWVDSSVIFGVVIINAMIGFV
                     QESKAESAIESLKKMMTTQTTVLRDGKWQEIDAVELVIGDIVQLASGNKVPADVRILR
                     CRDLQVDESALTGESIPVIKKEESLDPETILADRRNMAYAGTLVTYGQARAVVVATGD
                     KTETGRISELISSAADLSTPLTRKIASFSTILLIAILVLAAVTFAVGIWRGEGAVDMF
                     MAAVALAVGAIPEGLPAAVTITLAIGVNRMAKKRAIIRKLPAVETLGSTTVICSDKTG
                     TLTENQMTVKKIFAGDVFYDVDGNGYAPEGRLLRDGKKFTGELPPVLTQTLTAGLLCN
                     DSRLIQEEGQWRIEGDPTEGALITVAGKAGMTPETASAEMPRLDEIPFESERQFMATL
                     HRVKDRQNIIYIKGAVEKILDFCADGLDRDGGRAAVNKDDLFARVDAMAREGLRVLAL
                     ACKEIDKKNLDVEDCLSGLTFLGLQGMIDPPRTEAIAAVASCHKAGIMVKMITGDHAV
                     TATEIARQLNIGQDKGSQNSAPQTLTGKDLEKLTDEELIAAAGNTSVFARVAPEQKLR
                     LVEALQARNHIVAMTGDGVNDAPALKQANIGIAMGITGTDVSKEAADMVLTDDNFASI
                     TRAVEEGRRIFDNLVKFITWTLPTNGGEGLVILAAIFSGVTLPILPVQILWINMTTAI
                     LLGLMLAFEPGEPDVMDYPPRAPQSPILSWTLISRICLVIALMLAAVFGIFIWQKQQG
                     YSVEAARTVAVNLFIMIELAYLFNCRSLTKSMFTIGVFSNPWVLFGSAAMIVLQLLYT
                     YAPFMNRIFQSEPISGRDWLLIIAVSLAVHIVIGAEKWIRHHVFEQSHA"
     gene            5268..6557
                     /locus_tag="PLR13_06105"
     CDS             5268..6557
                     /locus_tag="PLR13_06105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQC18944.1"
                     /translation="MKDVVIVSGARTAVGEFGGSLKGIKTVDIGALVMKEAMKRAGLR
                     PAIDDFHKSCRPDAFGNFEMTDINKKYYDFDQSLQPVYVDEVIMGNVLQGGQGQNVAR
                     QACIYAGFPEETNAYTINKVCASGMKAIALAAQAIAAGDADIIVAGGMEVMSSAPFVV
                     PQARWGYRMSMPFSQITDMMVYDGLYEIFNGYHMGMTAENIAALYKISREQQDEFAFR
                     SHQRARAANASGAVADEIVPVVIPQKKGDPKVFNVDERPMDTSLEKMGKLPTVFKKDG
                     TVTAGNASGINDAAAAVVVMSAEKAKQLKLQPLVKISGYASGGVDPAYMGLGPIPASR
                     KLLKKLNLTTKDIDLFELNEAFAVQSLACMKELGVDADRCNLNGGAVSIGHPIGCSGA
                     RITYTLAMQMKKNNLKRGLASLCIGGGQGMTMILENV"
     gene            6814..8445
                     /gene="gpmI"
                     /locus_tag="PLR13_06110"
     CDS             6814..8445
                     /gene="gpmI"
                     /locus_tag="PLR13_06110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQC18945.1"
                     /translation="MALSLKKLKSFHGRKGPVLLIIMDGMGIGKADESNAVFMANTPH
                     LDTLFKSDLYTQLKAHGMAVGLPSDDDMGNSEVGHNAIGAGRIFDQGARLVNKALKSG
                     AIFQTAVWENIAKRAQRGGTVHFIGLLSDGNVHSHIEQLFIMIDKCAELNFRKVRIHP
                     LLDGRDVGERTALDYVVPTEEKLKKISGEKGFDYAIASGGGRMKVTMDRYNSDWTIVK
                     RGWDAHVRGEGRTFSSASEAVKTFYAEDSKATDQYLPPFVITDASGKPVGRIQDGDAV
                     IFFNFRGDRAIEISRAFSEKDFSEFDRGALPDIFYAGMMEYDGDAHIPHNFLVQPPAI
                     DRVISEYLCAEGVKTFAVSETQKFGHVTYFWNGNRSGYIDETLEKYVEIPSDRIEFDK
                     APQMKAREITDAVIDLLKSGHYRFGRLNYPNGDMVGHTGSADAAIAAVETVDQCVGAL
                     LTAVKDIGGIAVITSDHGNADEMFTIDKKGSKSVKTAHSLNPVPFIIYDPLYLGEYRM
                     ADLKEKGLSNIAATLLNLLGYEKPEDYDPSLIEMT"
     assembly_gap    8455..8554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8691..9473)
                     /locus_tag="PLR13_06115"
     CDS             complement(8691..9473)
                     /locus_tag="PLR13_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQC18946.1"
                     /translation="MEYKDILFNVEEEIATITFNRPKVLNALNTDVLTELLDAATICE
                     KDEKIKAVILTGAGDKAFVAGADIARMQNATSLEMVPVMELGHRTFRLIETMPKPSIA
                     AVNGFALGGGTELAMSCDIRFASEKAVFGQPEILIGVVPGWGGTQRLPRLVGMGIAKE
                     LILGGGQINAQRAYEIGLVNRVIPPENLMDEVRKFAVKLAKLPPFAMKMAKDAINYGY
                     DLSLDNAIKLEIACILQCFSTQDQKEGMKAFVEKRKPTFTGK"
     gene            complement(9541..11229)
                     /locus_tag="PLR13_06120"
     CDS             complement(9541..11229)
                     /locus_tag="PLR13_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC18947.1"
                     /translation="MAENISYASKPWLKSYEKGVPEIINYEELTMPDYLERSAKDFPN
                     DTAMVFVGYKMTYRQFKDQVDRFATCLTDFGIKKGDAVAILLPNSIPCVVAYYAILKI
                     GAIVVLNNPLYSDPELEHQFTDSDSKVLITLDALGNRMIDLRPKTKIKQIVYMGIGDF
                     LNPIKRFLGKKLKKFPFADVKAAPDVYRWMDCIKKYQPNPPKVKLTFDDIAMYQYTGG
                     TTGVSKGVILTHGNLSKQVQQINAWFPTFGRGGETMLGALPYFHVFGMSCSMNLSVAL
                     GWNQLLIPRPQPQPLLEAIREYKPTFTPLVPTMFIGMLNHPDLKKTDMSCIKGAFSGS
                     APLPVEVIQEFQKQTGSYISEGFGMTETCPVTHMNPFIGKQKIGSIGLPASDTLARIV
                     DLDDGVTDMPIGKPGELIIKGPQVMKGYKGMPEETATVLKDGWMYTGDIATMDEDGYF
                     YIVDRKKDMIISGGYNVYPRDIDEVYYGNPKVQEACSIGIPDAKRGENVKLFIVLKEG
                     QTATAEEMIEYGKAHLATYKLPTEVEFRKELPKSTVGKVLRKELRAEELKKRKG"
     gene            complement(11480..13645)
                     /locus_tag="PLR13_06125"
     CDS             complement(11480..13645)
                     /locus_tag="PLR13_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HQC18948.1"
                     /translation="MEHHGISPIGIGNEGRQVFWNAHSFEPFLFLFTAIAMAIFAYGV
                     YKRWKLWKAMGKDEIRVDELPARIKSLIMNGFFQVKTWKDIYPGIMHGCIFFGFFVLI
                     FGAAFDAGEFHITEPLFSWAFLRGSFYLGFSFLMDLFGLLVLIGVLMAAYRRYIQKPD
                     RLTYKGTPDTTPEDGIVLLLIGGIIVTGFIIEALRIHATLDPLTGYATWEGWSFVGWT
                     LANVISGLDIEAAKTWHSISWWTHTFIALGFIAYIPYSRLMHIITTPANHFFATLKPT
                     GYVEPIRDIENQESFGVSKLDEFTWKQIFDSDACTKCGRCQDGCPAYLTGKHLSPKKL
                     VQDIKTFWLEQAPLAATKAVVPVAEGAEAAAPAEAAEGETPEKALIGDVVDLNAVWDC
                     TNCMYCMENCSSSIEHIQKIIDMRRYKVLTEADFAPELQLTYRNMENNSNPWGIGGHL
                     RGDWAKELGIKTLAEDPNVEYLFYVGCSGSFDDRGKKISVAFAKILQAAGVSFGILGT
                     EESCCGDSAMRGGNDYLFQMQAQANIEVMNGYGVKKIIAICPHGYNCIKKDYPNFGGN
                     YEVYHHTEIIADLIAKGKIKFTQPLNGTFTYHDSCFLGRYNKVYAQPRSILNAIPGTK
                     LVEMERNLSKSFCCGAGGARMWMEEHGDRINNARTDQAIAVNANTVAVGCPFCLTMIS
                     DGIKDRQKIDSMIALDIAEIVAQAMGIEEVKVNFDTCSV"
     gene            complement(13803..14774)
                     /locus_tag="PLR13_06130"
     CDS             complement(13803..14774)
                     /locus_tag="PLR13_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQC18949.1"
                     /translation="MAKGIYVIAEQRDGALRKVSFELVSAARKLADQTSDEVCAILLG
                     SGVESMAAELGKFGADKVFVGDNAALEPYITEAHAQVVAKILKDNDAAIALFGASVQG
                     KDLSARVAAKLAGGLATDCTDVKIDGGKLVAVRPMYAGKCFGEVAFNTTPAMASLRPN
                     VFPAVENAKAGAVTKVDVAVDAQKSKILEVQKDTSGKVDLTEANIIVSGGRGMKGPDE
                     YKILEELADVLKGTVGASRAAVDAGWRPQKDQVGQTGKVVSPNLYIACGISGAIQHLA
                     GMSSSKCIAAINKDAEAPIFAKADFGVVDDLFKVVPELTAVCKKMLA"
     gene            complement(14798..15568)
                     /locus_tag="PLR13_06135"
     CDS             complement(14798..15568)
                     /locus_tag="PLR13_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQC18950.1"
                     /translation="MNIIACVKQVPDTEAQIKVKADGSGIDDSQIKWVMNPYDEFGVE
                     EALKLKEKNGGDVTIVSVGPARAMETIRTALAMGADKGIHISDPALDNADAFVTASAL
                     AAAIKGLPHDIIFCGQRAIDDDSGQVGAMLAELLGIPQLTLVTKLDYAGSSVKVIRPI
                     EGAQLSIETSLPCVVTAQKGLNEPRYASLPGIMKAKKKPVDVKNAAALGIATDVKANV
                     AKMVPPPARAAGKIICGDDAAQKAAELARCLREEAKVI"
     gene            complement(15687..17657)
                     /gene="acs"
                     /locus_tag="PLR13_06140"
     CDS             complement(15687..17657)
                     /gene="acs"
                     /locus_tag="PLR13_06140"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQC18951.1"
                     /translation="MADAKVQLKEIYPIPEKAKKKAWISGRDAYDKLWKKSVEKPEEF
                     WAEIAEQQVTWFKKWDKVMDYNFDIKKGPIYVKFFEGGKLNVSYNCLDRHLEKRGNQV
                     AIQWEGNEPGEERAITYKQLHEEVCKFANVLKAKGVQKGDRVCLYMPMVPELGIAALA
                     CSRIGAVHCIVFGGFSADALRDRIVNGAAKVLVVCDGTFRGSKAVPQKATADEALKSC
                     PSITSVIVVNRVGDKIKCDMTAGRDTWYHEEVAKADAKCEPEWMDAEDPLFILYTSGS
                     TGQPKGVMHTTGGYLVYGSYTHKIIFDYHDGDMYWCTADLGWVTGHTYILYGPLCNGA
                     TSIMFEGVPNYPTYSRFWQVVEKYKVNIFYTAPTAIRAIAKEGDEFVKKCDISSLQTL
                     GTVGEPINPEAWNWYFNLIGRGECPIVDTWWQTETGGILITPLPGAIDIKPGMATLPF
                     FGVQPVLVDDEGKEFNDTVASGALCIKVPWPGIMRGVYGDPKRFQETYFEQFPGYYVT
                     GDGCRRDKDGYYQITGRIDDVINVSGHRMGTAEVESALVAHKAVAEAAVVGMPHDIKG
                     QGIYAYVTLKTGVEKSDALKKELIAHVRTVIGPIATPDKIQWADGLPKTRSGKIMRRI
                     LKKVAANDLGNLGDTSTLADPSVVDDIVKNRL"
     gene            18422..19798
                     /locus_tag="PLR13_06145"
     CDS             18422..19798
                     /locus_tag="PLR13_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18952.1"
                     /translation="MKKLWLVLLSLGLLMAFSVSASAADVKFSGEYYAAGLYMNHVNL
                     QDPALTYSRSTAFFYQRLRLGTDFVVSPCLRLVTRMDIMERVWEPDNTGNDAFGRPAA
                     PGITNDARNLDFDVVYIDYVSPIGKFQIGYQPDYMWGTIWANRTTGPTAPQIKYFLPI
                     GPVVLFAGFAKEDENDYSVQYPGAVETDRDQNSYRVGPIFQFKGNNASGEAGVLFTYD
                     DVRQYGAVFEQDVFTIMPYFKTKFGPVSLQGEINYALGKREFEMPGVDDVDINSFSAF
                     LDGDVKLGVFDFGGSFAFVQGQDPDDMGMTDEINSQLSGGRDWDPCLILFNNTTQVSW
                     IAPNLTTWVPAALAGPNYKIGPTGEMRNAYFGQLRFGVKPVPKLRLATSVSYAAAHEK
                     PNVGFGDFDRDYGWEVDVTGTYKITNNLSYMLGGGYFFTGDYFQAGNDALDLNDDFII
                     INKLTLAF"
     assembly_gap    20528..20627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20783..20962)
                     /locus_tag="PLR13_06150"
     CDS             complement(20783..20962)
                     /locus_tag="PLR13_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18953.1"
                     /translation="MIAENNFADFALAADQQTDLPVDFTGQRRDLPGKIIRNDVFRRD
                     TFISEALQLFDVRRF"
     gene            21008..22387
                     /gene="hflX"
                     /locus_tag="PLR13_06155"
     CDS             21008..22387
                     /gene="hflX"
                     /locus_tag="PLR13_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQC18954.1"
                     /translation="MRFKGLRLIHTHLNGENLSDEDMTDLSHLRLDLIGALDVREDGS
                     PGLLHWAHLVPENPAGDYWLFMKPEEPHQIKINFLSFIQALEDEFAKKQQSRKIDATN
                     KAILLRVEKNPLAGADASLEELAQLAHTCGVEVFDSMIQYRPQPDPRFMVGRGKLSAI
                     DLRASQIGANMLIFDHDLTPAQARSISDFTGLKIIDRTQLILDIFARRAHSREGKIQV
                     ELAQLKYRLPRLTHADTSLSRLAGGIGGTGPGETKLEIDRRRVRERIHRLEKDLKSIT
                     KSRRQRYGRREKTGLPIISIVGYTNAGKSTLLNTLTHSSVLAEDKLFATLDTKSARLR
                     FPRDTEAIITDTVGFIRDLPEELFTAFRATLDELFEADILLHVIDASNAQFEDQIAAV
                     EKILTDLEIHDRPTIRVLNKSDRVADKELLKHLCRRLDAVAVSALDKKTLFALMEKIE
                     SVLPRKDDF"
     assembly_gap    22640..22739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22862..24061
                     /gene="porA"
                     /locus_tag="PLR13_06160"
     CDS             22862..24061
                     /gene="porA"
                     /locus_tag="PLR13_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HQC18955.1"
                     /translation="MYMIENGNTAAALGVKLCRAGVIAAYPITPQTPLTEKLSEYVEL
                     GEMKAEYIPVESEHSALAVCIAASSAGARVFTATSANGLLYMHEQIQWASGARLPIVM
                     CVVNRAVGAPWNVWNDQQDSISQRDTGWIQIYCCDHQQIIDTVIKAYWLAEKISIPIM
                     VCYDGYILSHTYMPFDLPEQEKVDAFLPPFQPDYALNPDEPANLNTVTLPDVRIDTKG
                     QLAHGYMEIRYLLQEELRTTLQIAADAEKRFAEIFGRGGNPYIEPYRCDDADYVAVGL
                     GSITYQLRVCVDTLRKEGIKVGVMGVRYYRPFPDAVIAGALKGKKGIIVFEKALSYGY
                     EGALASDLKSALYEHLAGTGSLPAVQNLIAGIGGREIHTDDLTQNLRAATQGRISREP
                     AWIGLKI"
     gene            24112..25014
                     /locus_tag="PLR13_06165"
     CDS             24112..25014
                     /locus_tag="PLR13_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQC18956.1"
                     /translation="MQAKTNILNLPTQEFVHPGTRACTGCGLSIAYRVGLKALGKDTI
                     LVVPPSCLTVLQGLFPVASTKLPCLNVTFASTAAAATGILSTLKTKGNDHTTVAAWAG
                     DGGTSDIGLQALSGAAERGDNFIYFCYDNEGYMNTGVQRSGTTPIGAVTANTPFKGKL
                     QQKKDVPAIMAAHKLSYVAACSAAYPLDLYDKIKSCLHLPGTKYFHIHIPCPPGWGYD
                     PRYGIKIGRLAVETGYYDLYEIVNGEFRLTAASEKLIEKRKLTPVREYFRAQSRFRIL
                     TDEQINEIQRQIDMKWDGYYKKGE"
     gene            complement(25081..26907)
                     /locus_tag="PLR13_06170"
     CDS             complement(25081..26907)
                     /locus_tag="PLR13_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC18957.1"
                     /translation="MAIFEYGEYSNVYEPPDNLVDFFESSAAKYTNNPLIGEKDSAGV
                     YQWVTYGDIARRVDHLRAGLASIGVGAKDTVGIIANNRKEWLIGEIATQGLGAAYVPM
                     YEKELVSMWKYIVKDAEIKVLLVSRSEILDKVKDFPAEIPSLKDIYLIEGEGEKTMAA
                     LERKGAANPVPSRKPKYSDVAVLIYTSGTTGEPKGVLLSHGNLAENVKAGLAWFPNLT
                     QQSRCISMLPWAHSFGITADLHAFILQGASIGIMGSVETLIDDLPKIQPTFMITVPRI
                     FNRVYNGVWAKMREEGGLKLKLFEAALEAAKRKRETGETGWKYKILDKIVLKKIRERF
                     GGRLENAVTGSAPMNVDIAKFFIDVGIPTYDAYGLSETSPAITINSPLMGNRLGSVGK
                     PINKTKVVIDRSRTGEDGDDGEIICFGPQCMIGYHKKPEQTQEVIVERDGMRGVRTGD
                     RGRLDDDGFLYITGRFKEEYKLSNGKYIHPDTIEREMKILPWVLNAVVTGAGHEFNVG
                     LIVPDMAVLKRVAEEMDLSVDPQAIFDPSSPAGRKLADLLTIEVQNHLKKIIGSYEVP
                     RKFAFIKEDFTLDNGMLTQTMKLKRKVVMDKYGEMLESLYRE"
BASE COUNT         6519 a         7011 c         6697 g         6581 t
ORIGIN      
        1 tcgcttgcct gaaaaaaaag acgcgcaggc ggtaaggcgg cttatcgaag attctccccc
       61 tctggatctg aattcgctgt actgttacct tctgctctgc acgcatttcg cgccgacatc
      121 ggcaattgcc gagtatcatc aggaggtttg cggattcgtt tccgcttata tccgcccgga
      181 tcaaaaagat accctgtttg tctggcaggt ggcggtccaa agggatttcc ggggaaaatc
      241 catcgctaaa ggcatgttgt tatcgtgtct gcagcgcgct catttatctt ccctgcgtta
      301 tctggaaacg acggttaatc cgtccaactc atcatcgaac gccctgtttt cctcgctggc
      361 cgaatttcta aatgccggtc tggccaaaag cgctttgttt tccgcggatg atttcggaaa
      421 aacgtctcat gaggaggaaa ttctgtatcg tatcgggccg ttcaacattt ctaaggaagg
      481 agaatctata aatgaaaaca attagacgac tggaatccga ggttcgcagt tattcgagat
      541 ctttccccgt tttgttcgaa aaggccaagg acgccttttt gtttgacgca ggcggaaccc
      601 gttatatcga ttttttcgca ggcgcgggga cgatcaacta cggacataac aacgaataca
      661 tgaaaaggaa aattttcgat tatctggaaa acgacggcat cgttcacggt ctggacatgg
      721 ccacggttgc caaggaggag ctgctcctca aaatggaggc caccctcttc aaaccgaaaa
      781 atcttgatta caaggtgcag ttcacgggtc ccacgggaac gaacgccgtg gaggcagcgc
      841 tcaaactggc ccgcatggta aagaaacgct ccaacattat ctcttttacc aacgggtatc
      901 acggtctgac gatgggctcg atgtccgtga ccgccaacgc ctactaccgc gacgaggcgt
      961 tcatcaaccg ttccgatgtt tcctttatgc cctacgacgg atatctgggt aaacacgtca
     1021 acaccgccaa gtatctgcgg cggtttctgg aagataacag cagcggggtc gatattcctt
     1081 cggccatcat tctggaaacg gttcagggtg aaggcggcat tcgcgtggcg ggggatgaat
     1141 ggctgcgcga ggtggaaaaa atctgccggg acttcgatat cctgctgatn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cttccgcaaa gggaaaattc tgcacaagcg
     1321 gctcaataaa atcagcgcga aatatccgca gcttcacacg gaggtgcgcg ggcggggttt
     1381 tgtctacgga atgggcctgt ccaccggcga gatcgccaag aagattctga ccgaatgttt
     1441 ccacaacaaa ctgattctgg aatcggccgg cgccagaaac aacgtcatca aatttcttgc
     1501 tcctctgacg atcacggatg aagtgctgga agaaggcctg gatattctgg agcgcagcat
     1561 cgacaacgtt atgaaaaaac actgaaagga tttctcatgc tcgtaagaac aattgacgat
     1621 atccggggaa cccgccggga ggtattcgcc gaaaataaaa actgggtcag ccgcagactg
     1681 cttttaaaaa aagacggcat gggattttcg tttcatgaaa cgatcatctt tgccggcacc
     1741 gaaacgcaca tctggtacaa gcatcatctg gaagcggttt tttgcgtaga gggagaaggc
     1801 gaggtcgaaa cggttgcgga cggaaaaatc caccgcatca cacccggcac attatacgcc
     1861 ctgaacgacc acgacgaaca ttatctgcgg gcccaaaagg atatgcgcct gttgtgcgtt
     1921 ttcaacccgc cggtcaccgg ggaagaagtt catctggcgg acggatccta tcctgcgccg
     1981 gaatagtgga acgtcctgcc ccctgtcggc agccggaatt ctttatgcgt gcgactgttc
     2041 aaaaacgtga tgccggatcc atttttccgc accgatcacg atatgaacgg ccagggaaac
     2101 agcgatgatc aacagccagt ccctgccgga aatgggctcg ctctggaaaa ttctgttcat
     2161 gaacggcgca taggtgtaga gcaattgcag aacaatcatc gccgcactgc cgaataaaac
     2221 ccacgggttc gagaaaacac cgattgtgaa cattgacttg gtcagagagc ggcagttgaa
     2281 caaataggcc agttcgatca tgatgaaaag attcaccgcc acggtgcgcg cggcctctac
     2341 ggaatagccc tgctgtttct gccagataaa aatgccgaat accgccgcca gcatcagggc
     2401 aatcaccagg catattctcg atataagcgt ccagctcagg atggggcttt ggggcgcccg
     2461 cggagggtaa tccataacat ccggctcgcc gggctcaaag gccagcatca gacccagcag
     2521 aatcgcggtc gtcatgttaa tccagagaat ctgtacaggt aatatgggca gggtgacgcc
     2581 gctgaaaatg gccgccagga tcaccaggcc ttcaccgccg tttgtcggca atgtccacgt
     2641 gataaatttt acaagattat caaaaattcg acggccttcc tccaccgccc gcgtgatgct
     2701 ggcaaaatta tcatcggtga gcaccatatc ggccgcttct ttggacacat ccgttccggt
     2761 aatgcccatg gcaatgccga tgttggcctg tttcagcgcg ggcgcatcgt tgacgccgtc
     2821 gccggtcatg gcgacgatat gattgcgcgc ctgcaaagct tccaccagcc gcagcttctg
     2881 ttccggagcg acgcgcgcga aaacggatgt gttccccgct gcggcgatca gttcttcgtc
     2941 ggtcagtttt tcaagatcct tgccggtcag tgtctgcggc gcactgtttt gactgccttt
     3001 atcctgcccg atgttcagtt ggcgggcgat ttccgtggcc gtgaccgcgt ggtccccggt
     3061 aatcatttta accataatcc ccgccttgtg acaggacgca acggctgcaa tcgcctccgt
     3121 gcggggagga tcaatcatcc cctgcagtcc cagaaatgtc agaccggaaa ggcagtcttc
     3181 gacgtccaga tttttcttgt cgatttcctt gcaggccagc gcgagaacgc gcagtccttc
     3241 gcgcgccatg gcatccaccc tggcgaataa atcgtccttg ttcacggctg ccctgcctcc
     3301 gtcacgatca agaccgtcgg cgcagaaatc caggattttt tccaccgcgc ccttgatgta
     3361 gattatattt tgcctgtcct ttacacggtg cagcgtggcc atgaactgcc gttccgattc
     3421 gaaaggaatt tcgtcaaggc ggggcatttc ggcgctggcc gtctccggcg tcataccggc
     3481 tttcccggcc acggtaatca gcgcgccttc cgtgggatcg ccttcaatac gccattgacc
     3541 ttcttcctga atcaggcgcg aatcgttaca cagcagtccc gccgtcagtg tttgcgttaa
     3601 aaccggcggc aattcccccg tgaatttttt tccgtcacgc aagaggcggc cctccggtgc
     3661 gtagccgttg ccgtcaacgt cgtaaaaaac atctcccgcg aaaatttttt tcacggtcat
     3721 ctggttttcc gtcagcgttc cggtcttgtc cgaacagatg accgtggtgc tgcccagggt
     3781 ttccacagcg gggagtttgc ggatgatggc tcttttcttc gccatccggt taacgccgat
     3841 ggccagcgta atggtgacgg cggcaggcag tccttcggga atcgctccga cagccagggc
     3901 taccgcggcc ataaacatat ccacggcccc ttcgccgcgc cagataccca cggcaaatgt
     3961 caccgcggcc agaacaagaa tggcaataag aaggatggtg ctgaaagaag cgattttccg
     4021 ggtcaggggc gtggacagat cggccgctga agaaatgagc tctgaaatac gccccgtctc
     4081 ggttttgtcg ccggtggcca caacaacggc gcgcgcctgc ccgtaggtta caagggtccc
     4141 ggcataagcc atgttcctgc ggtcggccag gatcgtctcc ggatcgagag actcttcctt
     4201 cttgataacc ggaatcgatt ctcccgtgag ggcggactcg tccacctgca gatcgcggca
     4261 gcgcaaaatc cggacgtcgg cgggcacttt attgccggaa gccagttgga caatatcgcc
     4321 gatcacaagt tccaccgcat cgatttcctg ccacttgccg tcgcgtaaca cagtcgtttg
     4381 cgtcgtcatc atttttttca gcgattcgat ggcactctcc gccttcgatt cctgcacaaa
     4441 gccgatcatg gcattgatga tcacaacgcc gaagatgacg gacgaatcca cccattcctg
     4501 caagaaaaag gttactgccc cggccgccat cagaatgtaa atcagcggtt gatggaactg
     4561 gagaagaaac cgcatcagcg gccctttctg ctttttcatg gtgacagaat tgggaccgta
     4621 tttttccagc cggtgtttgg cctcaaaagg cgaaaggcct ttgtccgcat cggtattgag
     4681 caacgttgta acttcttcag agggacagtg gtgccagtgc ttgcatataa tggattccat
     4741 gaagcttctc ctgatttttt ttgccgtaac accctgcgaa gaggggtata gcggtgacgt
     4801 cggttgaagg ttcggtaatg agcgtgatga acaaaaagat tcttaaggtt tgtaagcagt
     4861 gtagcctatg cgacgtgcat ttgtcaattt aaaccggaca tttttttgaa taaccggcag
     4921 acatgcggac acattagcaa gttttttcat ccggaattat ttttccaccg cagaccggat
     4981 ggtttgccga cgctaaaatc gttgcgcttc caaaacgaaa aatacaagaa ataagtaatg
     5041 aaattaaagt tttgacaacc aggcaggtga tgacatcggc ctgatgaaac ggttcagacg
     5101 tttacaaaaa aacgatccgg tttttcttga atctgtccaa ccttgcttga tgcattttat
     5161 tggaaattat gctaacaatc gcatggttct atggaagaaa tgcccgtcgt tgccgggttg
     5221 tactcaacag ggctataaaa tattttgaac attgaaagga gttaagcatg aaagatgtag
     5281 taattgtgag cggcgcccga accgctgtgg gagaattcgg cggatcgttg aaaggtatca
     5341 agacggttga tatcggggca ctggtgatga aggaagccat gaaacgcgcc gggctgagac
     5401 cggccattga tgattttcat aaaagctgcc gtcccgatgc cttcggcaat ttcgagatga
     5461 cggacatcaa taaaaaatat tacgactttg atcaatccct tcagcctgtt tacgtggatg
     5521 aagtcattat gggcaatgtt ctgcagggcg gacagggaca gaatgtcgcc cggcaggcct
     5581 gtatttatgc gggttttccg gaagaaacca atgcctatac gattaacaag gtctgcgctt
     5641 cgggcatgaa agccattgca ctggccgctc aggcgattgc ggccggcgat gccgacatta
     5701 ttgttgccgg cggcatggaa gtcatgagca gtgcgccgtt tgtcgtgccg caagcccgtt
     5761 ggggctaccg gatgagcatg cctttttccc agattaccga catgatggtc tatgacggtc
     5821 tctatgaaat tttcaacggc taccacatgg gaatgaccgc ggaaaacatc gcagcacttt
     5881 acaaaatctc ccgcgagcag caggacgaat tcgctttccg cagccaccag agagcccgag
     5941 cggcgaacgc ctccggcgcc gtggctgatg aaatcgtgcc ggtggtcatt ccgcagaaaa
     6001 aaggcgaccc caaagtattt aacgtcgatg aacgtcccat ggatacgtct ctggaaaaaa
     6061 tgggcaaact gccgacggtg ttcaaaaaag acggcacggt taccgcggga aatgcatccg
     6121 gtatcaacga tgccgcggcg gctgtggtgg tgatgagcgc cgaaaaagcc aaacagttga
     6181 aacttcagcc gctggtcaaa atcagcggat acgccagcgg cggtgtggat cccgcgtaca
     6241 tgggactagg accgattccg gcatcgcgca aactgctcaa aaaactgaat ctgaccacaa
     6301 aggatatcga tcttttcgaa ctcaatgagg cctttgcggt gcagtccctg gcctgcatga
     6361 aggaactggg tgtggacgca gaccgatgca atctcaacgg cggcgcggtt tccatcggcc
     6421 atcccatcgg ctgctccggc gcccgcatta cctacacact ggccatgcag atgaagaaga
     6481 acaatctgaa acgcggtctg gcctcgctgt gcatcggcgg cggtcagggc atgaccatga
     6541 ttctggaaaa cgtttaaaat gtttttgtca aaacacaggg agggtggatt ctctaccctc
     6601 cctgctcatc ccactctgtt ataccaagga gtattgccat gtcattaagt tcggtcgagt
     6661 ctttttgact cgaccgttca tcaacagcca agtcaaaaag acttggctca accgatgttt
     6721 tgccatgaca tttcttttct tgaccgagta tctactttta tctataaaga aacctatgaa
     6781 aaatttcagc cgatattatc agggagttga tcaatggcat taagtctcaa gaaattaaaa
     6841 tcttttcacg ggaggaaagg acccgttttg ctgattatca tggacgggat gggcatcggg
     6901 aaagcggatg aatcgaacgc cgttttcatg gccaataccc cccatcttga cacattattt
     6961 aaatcggatc tgtacacgca gttgaaagcc cacggcatgg ccgtcggcct gccgtcggat
     7021 gacgatatgg gcaacagcga agtcggccac aatgccatcg gcgccgggag gattttcgat
     7081 cagggcgccc gtctggtcaa caaagctctg aagagcggag caatctttca gacagcggta
     7141 tgggaaaata tcgcaaaacg cgctcagcgc ggaggaacgg ttcatttcat cggcctgtta
     7201 tccgacggca acgtccactc gcacattgag cagctgttta ttatgatcga caaatgcgca
     7261 gaattaaatt ttaggaaggt tcggattcat cccctactgg acggcaggga cgtcggagaa
     7321 cggacggcgc tggactatgt tgttcccacc gaggaaaaac tcaaaaaaat ctccggcgaa
     7381 aaaggcttcg attacgccat cgcctcggga ggcggacgca tgaaggtcac catggaccgg
     7441 tacaattccg actggacgat cgtgaaacgc ggatgggatg cgcacgtacg gggcgaaggc
     7501 cggacgtttt cctccgcatc ggaggcggtc aagacgtttt acgcggaaga ttccaaggca
     7561 acggatcagt acctgccgcc gtttgtcatt accgatgctt cgggaaaacc ggttggtcgc
     7621 attcaggacg gcgacgccgt gatctttttc aacttccgcg gcgaccgcgc catcgagata
     7681 tccagagcct tctccgaaaa ggatttttcc gaattcgacc ggggagcact gccggatatc
     7741 ttttatgcgg ggatgatgga gtatgacggc gatgcgcata tcccgcacaa ttttctggtg
     7801 caacctccgg cgattgatcg cgtcatcagc gagtatctct gtgcggaagg cgtaaaaacg
     7861 tttgcggtgt ccgaaaccca gaagttcggt catgtgactt atttctggaa tggcaaccgt
     7921 tccggttata tcgatgaaac gctggaaaaa tatgtggaga ttccttccga ccggattgaa
     7981 tttgacaagg ccccgcaaat gaaggcaagg gaaataacgg atgcggtgat tgacctgttg
     8041 aaaagcggcc attaccggtt cggcagactt aattacccga acggcgacat ggtcgggcat
     8101 accggatcag cagacgcagc cattgccgcg gtggaaaccg tggatcaatg cgtgggcgcg
     8161 ctgctgaccg ccgtcaaaga catcgggggc attgccgtca ttacctcgga tcacggcaac
     8221 gcggatgaga tgtttacgat cgataaaaag gggtccaaat ccgtcaagac cgcgcactcc
     8281 ctgaatccgg tgccttttat tatttacgat cccctgtatc taggtgaata ccgcatggcg
     8341 gatcttaaag aaaaagggct gtccaatatt gcggcaaccc ttttgaatct tctgggatac
     8401 gaaaaaccgg aagattacga tccgtcactg attgaaatga cttaacaaac gttcnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgaaaa accggaagat tacgatccgt
     8581 cactgattga aatgacttaa caaacgttct ccgttctacg ttctacgttc tacgttctat
     8641 gttctacgtt acatttttta acgttgaact ttgaacgtag aacattgagt ttacttcccc
     8701 gtaaacgttg gttttctttt ttccacaaat gctttcatgc cttctttttg atcctgggtg
     8761 ctgaaacact ggaggataca ggctatttcc agtttgatgg cgttatccag cgacaggtcg
     8821 tacccgtaat tgatcgcgtc tttggccatc ttcatggcaa agggcggcaa cttggccagc
     8881 ttcacggcga atttccggac ttcatccatg agattttccg gaggaatgac tctgttgacc
     8941 agtcctattt cataggcgcg ctgggcgttg atctgcccgc cgcctagaat cagttccttg
     9001 gcaatcccca tgccgaccaa ccggggaaga cgctgtgtgc cgccccatcc gggaacaaca
     9061 ccgatgagaa tttccggctg gccgaaaacg gctttctccg atgcaaaccg gatatcgcag
     9121 gacatggcaa gttccgtgcc gccgcccagc gcaaaaccgt tgacagcggc aatagacggt
     9181 ttcggcatgg tttcgatcag cctgaatgta cgatgcccca attccatgac gggaaccatt
     9241 tccagggatg tggcattctg catccgcgcg atatccgctc cggccacaaa cgccttgtct
     9301 cccgcaccgg ttagaattac tgccttgatt ttttcatcct tttcgcagat ggtcgcagcg
     9361 tccagaagtt ccgttaacac gtcagtgttc agggcgttga gaactttagg tctgttaaat
     9421 gttattgtag caatttcctc ttcgacgtta aaaagtatgt ctttgtactc cattttctcc
     9481 ctctttcttg tgaaaatgcc ggaatacggc aaatcgcatt ccggcattca cattttttta
     9541 ttaacctttt cttttcttta attcttccgc ccgcagttcc ttgcggagaa cctttcctac
     9601 cgtgctcttg ggaagttctt tgcgaaattc cacttccgtc ggcagtttgt aggtcgccag
     9661 atgagccttg ccgtattcga tcatttcttc cgccgtggcg gtctggcctt ctttcagaac
     9721 gatgaagagt ttcacattct ctccgcgctt cgcgtcgggg atgccgatgg agcaggcctc
     9781 ctgaactttc ggatttccgt aatagacttc atcgatatcc cgcgggtaaa cattgtaccc
     9841 gccggaaata atcatatcct tcttgcggtc aacaatataa aaatatccat cctcgtccat
     9901 cgtggcaatg tctccggtgt acatccaccc gtctttcaaa acggtggccg tttcctcagg
     9961 cattcccttg taccccttca taacctgcgg ccccttgatg atcagttctc cgggttttcc
    10021 aatcggcata tccgtaacac cgtcatccag atcgacgatc cgggcaagag tatcagaggc
    10081 aggaagtccg atgctgccga tcttctgttt tccaataaac gggttcatgt gcgttaccgg
    10141 acaggtttcc gtcatgccga acccttcgga aatataagac ccggtctgtt tctggaattc
    10201 ctgaattact tccacgggca ggggcgcgct gccggagaaa gctcctttga tacagctcat
    10261 gtccgttttc ttcagatcgg gatggttgag catgccgata aacatggtcg gtaccaacgg
    10321 tgtaaatgtg ggtttgtatt cccgaatggc ctccagaagc ggctgcggct gcggccgggg
    10381 aatcaggagc tgattccagc ccagggcgac cgacagattc atggagcagg acattccgaa
    10441 cacatggaaa tacggcaatg cgcccagcat ggtttctcca ccgcggccga atgtcgggaa
    10501 ccaggcgtta atctgctgca cctgtttgct taaattccca tgcgtcagga taacgccttt
    10561 ggacacaccg gtcgtaccgc cggtatattg gtacatggcg atatcgtcga aggtcagttt
    10621 cactttcggc ggattcggct gatatttttt aatgcaatcc atccagcgat acacgtccgg
    10681 agccgctttg acatcggcga aggggaattt tttcaatttt ttaccaagaa atcgcttaat
    10741 cggattcagg aagtccccga tgcccatata aacaatctgt ttgatttttg ttttcggccg
    10801 caaatcaatc atgcggttcc cgagggcatc gagcgtaatc agaaccttgg aatcggagtc
    10861 tgtaaactga tgttccagtt cgggatcgga atagagcgga ttattcagaa caacaatcgc
    10921 accgatcttt aaaatagcgt agtaggccac tacacagggg atgctgttgg gaagaagaat
    10981 cgccacggca tctccttttt taatgccgaa atccgtcaga caggtggcaa aacgatctac
    11041 ctgatctttg aattgacggt atgtcatttt ataaccgaca aataccatgg ccgtatcatt
    11101 gggaaagtct tttgcggatc tttcgagata gtcgggcatc gtcagttctt cataattgat
    11161 gatttcagga acgccctttt cgtaggattt cagccagggc ttgctcgcgt aagagatgtt
    11221 ttctgccata atactcccct ccccttaata aaattttttg gaatggcgca ttatactctt
    11281 ctgaagtata aattcaaata tatttttaaa tccggcccgc aagaacaggc aaagtggagc
    11341 ccggagcgcc gggaaaaagg agccgcgttt gaatttcaaa aacaaagcgg caaaatgaca
    11401 ggacgttgca aacatcctta taaaaaaagc ccggctggac atcaagccgg gcttttacaa
    11461 aacatcgatg aatgtttact tacacagagc aggtgtcaaa attgaccttc acttcttcga
    11521 tacccattgc ctgggcaaca atttcagcaa tatccaatgc aatcatggaa tcgattttct
    11581 gtctgtcttt gataccgtcc gaaatcatgg ttaagcagaa cggacaaccg acagccaccg
    11641 tgtttgcgtt gaccgcaatc gcctgatcgg tcctcgcgtt gttgatgcgg tcgccatgtt
    11701 cttccatcca cattctcgct ccgccggcgc cgcagcagaa gctcttggac aggttgcgtt
    11761 ccatttcgac aagcttcgtt cccggtatag cattcagaat gctgcgcggt tgagcgtaaa
    11821 ccttgttgta acggccgagg aaacaggaat catgataggt gaaggttccg ttcaacggct
    11881 gtgtgaattt aatcttaccc ttcgcaatca gatcggcaat aatctccgtg tgatgataca
    11941 cttcgtagtt gccgccgaaa ttgggataat ctttcttgat gcagttgtaa ccgtgcggac
    12001 aaatggcgat gatcttcttc acgccatagc cgttcatgac ttcaatattg gcctgagcct
    12061 gcatctggaa caggtaatcg ttaccgccgc gcattgcgga atcaccgcag cagctttctt
    12121 ccgttcccag aataccgaag ctgacaccgg ccgcctgcag gattttggcg aacgccacag
    12181 aaatcttctt gccgcgatca tcaaaagagc cggaacagcc gacatagaag agatattcaa
    12241 cattgggatc ttccgccagc gtcttgatac ccagttcttt cgcccagtca ccgcgcagat
    12301 gcccaccgat accccacggg ttggagttgt tttccatgtt tctgtaagtc aactgcagtt
    12361 cgggagcgaa atcggcttcg gtcagaactt tgtaacgacg catgtcaata atcttctgga
    12421 tatgctcaat cgaggatgag cagttttcca tacaatacat acagttggtg caatcccata
    12481 cggcattcag atcgaccaca tcgccgatca gagctttttc cggggtttcg ccttcggccg
    12541 cttcagcggg agctgccgct tcagcacctt ccgcaacggg aaccacggct ttggtggccg
    12601 ccagaggcgc ctgttccagc cagaatgtct tgatatcctg aaccagtttc ttgggcgaca
    12661 aatgtttgcc ggtcagatag gccgggcaac catcctggca gcgtccgcac ttcgtgcagg
    12721 cgtcggaatc aaaaatctgc ttccaggtga attcatccag cttggacaca ccgaaggatt
    12781 cctgattctc aatatcacga atcggttcca catatccggt cggcttcagc gtcgcgaaga
    12841 aatggttggc cggtgtggta atgatatgca tcaaacggga gtatggaatg taagcaataa
    12901 aaccgagtgc aataaatgtg tgcgtccacc aactgatgga atgccaggtt ttggctgctt
    12961 caatatccaa gccggaaatc acattggcca gggtccagcc gacaaatgac cacccttccc
    13021 atgtcgcata accggtaagc ggatccaaag tagcgtgaat acgcaaggct tcaataatga
    13081 atccggtcac gatgatgccg ccgatcagta aaagaacgat gccatcttca ggcgtggtat
    13141 ccggagtgcc tttataggtg aggcgatccg gtttttgaat gtaccggcga taggcggcca
    13201 tcaatactcc gatcagcacc agcagaccga acagatccat caggaaggag aacccgagat
    13261 aaaaactgcc gcgcaaaaac gcccagctga acaacggctc cgtgatgtga aattctccgg
    13321 catcaaaagc ggctccgaaa atcaaaacaa agaaaccaaa aaagatacag ccatgcataa
    13381 ttcccgggta tatatctttc catgttttga cctggaaaaa tccgttcata atgagagact
    13441 ttattcgtgc aggaagctcg tccacacgga tttcatcctt gcccatcgct ttccagagct
    13501 tccagcgttt gtaaacaccg taggcgaata tcgccatggc aatggcagta aaaagaaaaa
    13561 ggaacggctc aaaactatga gcgttccaga aaacctgacg accttcattc cctattccga
    13621 tagggctaat accatgatgt tccattgatc ctccgaaata attgaggttt tttatgtatt
    13681 tttaacttaa tttttctttc aagtccgaag acctgaattg ataatttaat aaaaattctt
    13741 tgcggctgtt ttattgtaag cttccggcaa gggttctcct taccggaagc tttgtgaata
    13801 tcttaagcca gcattttctt gcagacggcg gtcagttcgg gaacaacttt gaacaagtca
    13861 tccacaacgc cgaaatcggc ctttgcaaaa atgggagctt ccgcatcctt attaatagcc
    13921 gcgatgcatt tcgatgagct catgcccgcc agatgctgaa tcgcaccgga aataccgcag
    13981 gcgatgtaca gattcggaga gacaacctta ccggtctgtc cgacctgatc tttctgggga
    14041 cgccatccgg catccaccgc cgcacgggaa gcaccgacgg tgcctttgag cacgtcagcc
    14101 agttcttcca gaatcttgta ttcatccgga cctttcatac cacgaccgcc ggaaacaata
    14161 atgttggctt cagtcaagtc aaccttgccg ctggtgtctt tctgcacttc cagaatcttg
    14221 ctcttctgag cgtcaacagc aacgtcaacc ttggtgacag cgccggcttt ggcgttttcc
    14281 accgccggga aaacattggg acgaagcgat gccatcgccg gagtggtatt gaaggccact
    14341 tcgccgaagc atttaccggc gtacatcgga cgaacggcga cgagtttccc gccgtcaatc
    14401 ttgacgtcgg tgcagtctgt cgccagaccg ccggccagct tggcagctac gcgggcggaa
    14461 agatccttgc cctgaacgga agcgccaaac aatgcgatcg ctgcatcatt atcttttaat
    14521 attttggcaa caacctgagc gtgtgcttcg gtaatatagg gctccagagc cgcattgtcg
    14581 ccgacaaaaa ctttatctgc accgaattta cccaattcgg cagccatact ctctacacca
    14641 gaaccaagca gaatggcgca aacttcatcg cttgtctgat cagccagttt tctggccgcg
    14701 gaaacaagct caaagcttac tttacgcagg gcgccgtctc tctgttctgc aattacatat
    14761 attccttttg ccatttttca attcctccgc ttttaaatta aataacttta gcttcttccc
    14821 tgagacaccg ggccaattcc gccgccttct gagccgcgtc atcaccacaa atgattttgc
    14881 cggctgctcg cgcggggggc ggaaccattt tggctacgtt ggccttaaca tcggtagcaa
    14941 tacccaaagc ggccgcgttt ttaacatcca cgggtttctt cttggctttc ataatgccgg
    15001 gaagtgacgc gtagcggggt tcattgagac ctttctgcgc cgtgacgaca cagggcaaac
    15061 tggtttcaat ggagagctgt gcaccttcaa tgggacgaat tactttcacg gaagatccgg
    15121 cgtaatccag tttggtgacc agggtcaact ggggaatgcc cagaagctcg gccagcatgg
    15181 cgccgacctg accggagtca tcatcaatgg cgcgctgtcc gcagaaaata atgtcatggg
    15241 gcaggccttt gatggcggca gccagagcag atgctgttac aaaagcatct gcgttgtcca
    15301 gagcgggatc actgatatga atgcctttat cagcgcccat ggccagagcg gttctgatgg
    15361 tttccatggc tcttgccggt cccacggaaa cgatggttac gtccccgccg tttttctctt
    15421 tcagtttcag agcttcttcg acaccgaatt catcgtaagg attcatgacc catttaatct
    15481 gggaatcatc tatccctgat ccgtcagctt tgactttgat ctgggcttcg gtatccggta
    15541 cttgttttac gcatgcgata atattcactt tgtccaacct ccttcttctg cgcatttttc
    15601 ctttaaagga aaacgcacaa ttttattttt ctctttattt cattaaaaag tccggttccc
    15661 atttccgggg aaccggactt aatgttttac agtctgtttt tgacaatatc gtcaaccacg
    15721 gacggatccg ccagcgtgct ggtgtcgccg aggttgccca gatcattggc ggcaactttc
    15781 ttcaaaattc ttctcatgat cttgcctgaa cgggtcttgg gaagaccatc agcccactga
    15841 atcttgtcgg gggtcgcgat gggtccgata accgtgcgga catgagcgat gagttctttc
    15901 ttcagcgcgt cggatttttc cacgccggtt ttcagggtaa cgtaagcgta aatgccctgg
    15961 cctttgatgt cgtgcggcat gccgacaacg gcagcttcag caacagcttt gtgagcgacc
    16021 agtgcgcttt ccacttcggc ggtgcccatg cggtgaccgg acacgttgat aacgtcgtcg
    16081 atgcggccgg taatctgata gtagccgtct ttgtcacggc ggcaaccgtc accggtcaca
    16141 tagtagccgg ggaactgttc gaaatacgtt tcctggaagc gcttgggatc gccgtaaacg
    16201 ccccgcatga tgccgggcca gggaactttg atgcacagcg cgcccgaggc gacggtgtcg
    16261 ttgaattcct tgccttcgtc atcgaccagc acgggctgta cgccgaagaa gggcagtgtg
    16321 gccataccgg gttttatgtc aatcgcgccg ggaagcggag taatcagaat cccgccggtc
    16381 tctgtctgcc accaggtgtc cacgatgggg cattcgccgc ggccgatcag attgaaatac
    16441 cagttccagg cttcggggtt gatcggttcg ccgacggttc ccagcgtctg cagagaagag
    16501 atgtcgcatt tcttgacgaa ttcgtcgcct tccttggcga tcgcgcggat cgcggtcgga
    16561 gcggtgtaga aaatgttgac cttgtacttt tcaacgacct gccagaaacg gctgtaggtc
    16621 ggatagttgg gaacgccttc aaacataatg gatgtggcgc cgttgcagag aggaccgtac
    16681 agaatgtaag tgtgaccagt gacccatccc aggtcggcgg tgcaccagta catatcgccg
    16741 tcatgataat caaaaataat cttgtgggtg taggaaccgt ataccaggta gccgcccgtg
    16801 gtgtgcataa cgcccttggg ttgtccggtg gaaccggagg tataaaggat gaacagcgga
    16861 tcttcggcat ccatccattc cggttcacac ttggcgtcag ctttagcaac ttcttcatga
    16921 taccaggtat cgcggccggc cgtcatgtcg cacttgatct tgtcgccgac tctgttgacc
    16981 acgatcacgg atgtaatgga cgggcaggat ttcaatgctt catcagcagt cgctttctgg
    17041 ggaacagcct tgctgccgcg gaaggtgccg tcgcagacaa ccagaacttt ggctgcacca
    17101 ttgacaattc tgtctctgag cgcgtcagcg ctgaatccgc cgaaaacgat acaatgaacg
    17161 gcgccgatgc gcgagcaggc cagagcggcg atgcccagtt cgggaaccat cggcatgtac
    17221 agacagacgc ggtcgccctt ctgaacgccc ttggctttca gaacgttggc gaatttgcaa
    17281 acttcttcat gcagctgttt gtaggtaatg gctctttctt cgcccggctc gttgccttcc
    17341 cactgaatcg caacctgatt gccgcgtttt tcaaggtgcc tgtccaggca gttgtaagaa
    17401 acattgagtt tgccgccttc aaaaaacttc acatagatgg ggcctttttt aatgtcaaag
    17461 ttgtaatcca tcactttgtc ccattttttg aaccaggtca cctgctgctc ggcgatttcc
    17521 gcccagaact cttccggttt ttcgaccgat tttttccaca acttgtcgta agcatctctt
    17581 ccgctaatcc aggctttctt tttcgccttt tccggaatcg gatatatctc ctttaattga
    17641 acttttgcat cagccatgat aatttattcc tccctaaaaa tttttatgat ttcaaacgtt
    17701 tttattacgc cttgataccg gccaaacaaa cattctaact gtttgatact aacggtgttt
    17761 ttgaacaaag gtcacaactt gactgaaagt cagagatgcc tcggattttt cctatcctaa
    17821 gatttatttg atgtcaagaa atatttaaaa atcacgtcct gcaatttatg aatttccctt
    17881 ttaaaaagcc ggaccggcct acagatgcct tttgacaatc aaaaatcaaa aacacaaatt
    17941 aaaaattatc ataataatat tatgctatta tccagacttt ttccgttggg tttttggcaa
    18001 atattcaaac ttccgaaaaa cggcccatac cttcatacag aaacttcaac ccccttgatt
    18061 cgatgcgatg gaattcaaac aaaaccgttt ctgctttact atgtatcgta cgacctcaat
    18121 caggtggaca ctgtaagcgc aaagcgagag attaatttta tccgcacaca aaaacacctg
    18181 tctccatttt caaatgatca aatttttatg attcgtcttt gggtttattt gaaaacattt
    18241 tcgaacgtaa aaacattctt gacaaaatga attattcagt gctattttaa tacagacgta
    18301 tttttgactg taattcacgg gagtttttcc cgtaatttat taagtgtgtg gaagtcgctt
    18361 gaagaaaatg cccgttttct gaaaagcggt aattaatgac tctttattta aggaggaaaa
    18421 catgaagaaa ctttggttag ttttgttgtc actggggcta ctcatggcct tcagtgtgtc
    18481 agcttccgca gctgacgtta aattcagcgg cgaatattat gccgccggcc tgtatatgaa
    18541 tcatgttaat ttacaggatc ccgcattaac gtattcccgc agcacagcct ttttctatca
    18601 gagactgcgc ctgggaaccg actttgttgt atcgccctgc ctcagactcg taacccgcat
    18661 ggacattatg gaaagagtct gggaaccaga caacacgggg aatgatgcat ttggcagacc
    18721 agctgctccc ggcatcacca atgatgcccg caatctggac tttgacgtcg tctacattga
    18781 ttatgtttct cccatcggca aattccagat cggttatcag ccggactata tgtggggcac
    18841 catctgggcc aacagaacca ccggaccgac cgctcctcag atcaaatatt ttctgccgat
    18901 cggacccgtt gtgttgtttg ccggatttgc aaaggaagac gaaaatgatt actcggtgca
    18961 gtatcccggc gcagtagaaa ccgacaggga tcagaattct taccgcgtcg gcccgatttt
    19021 tcaatttaag ggcaacaatg catccggtga agcaggcgtt ctgttcacct acgacgatgt
    19081 ccgtcagtat ggggccgttt ttgaacaaga cgtgttcaca attatgcctt atttcaaaac
    19141 caagttcgga cctgtttctc tccagggcga aatcaactat gctcttggta aaagagagtt
    19201 cgaaatgccc ggcgtggatg acgtagacat caactctttt tccgcatttc tggatggcga
    19261 tgttaaatta ggagtatttg acttcggcgg atcttttgct ttcgtccagg gccaggatcc
    19321 tgatgatatg ggtatgaccg atgaaattaa cagccagctt tccggcggtc gtgactggga
    19381 tccctgtctg attctcttca acaacaccac tcaggtcagc tggattgcac caaaccttac
    19441 aacgtgggtc cccgcagcac ttgcaggacc aaattacaaa atcggtccga cgggtgaaat
    19501 gcgaaatgcc tacttcggcc agttaagatt cggtgtgaaa ccggttccca aactgcgttt
    19561 ggccacatcg gtttcttacg cggcagcgca tgaaaaaccg aatgtcgggt ttggcgattt
    19621 cgatcgcgac tacggttggg aagttgatgt caccggtacc tataaaatca ccaacaacct
    19681 gtcctacatg ctgggcggcg gttatttctt caccggcgat tacttccagg caggcaatga
    19741 tgctcttgac ctcaacgacg attttatcat catcaacaag ttgactcttg ccttttaaga
    19801 gccatgtaaa atccggcaaa tgccggatga acagtaaaac caaccccggg aggaagtttc
    19861 ctcccggggt ttttagtgag gcaagctgaa gacgagtgca acgaagtctg aagcgtaagc
    19921 cgaactatcc cgcaagcgaa gcttgtcggg attgtggact tcaaatttca gaagcgaagc
    19981 gcactagaac ttgatggttg aactatcccg ctttagcggg attcctgaat ttaagaatca
    20041 acaacatcgc agtaatatct ggctggacga attaccccag gagcgcagcg acgtggggta
    20101 atgagccttg actataatct tagagcgaac tatcccagtc ccgcagggac gtgggaagtg
    20161 acactcgctt gaagcgctag tggaattatc ctaggagcga agtaccctaa agggcatctt
    20221 cgacgacgta ggaaatgaga cccgcttggt gcgaagcgga gtgggattgt gaacttcatt
    20281 ttataaacgc ctgccaccga atctagtaag ccgaactatc ctaggagtgc agcgacatta
    20341 ggaaatgacc ctcgccggta acaagcccct attcggtggg atgcagcttg aattttgaat
    20401 ccagaaccgt tttttctatt cactattcac tattcactat tcactattca ctattcacta
    20461 ttcaccatca atcatcaact gcccttcaca tcttttgact aaatcctgca aaatgtggta
    20521 aagcaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat caactgccct
    20641 tcacatcttt tgactaaatc ctgcaaaatg tggtaaagca atttcaacgc atcacatcct
    20701 ccactggccg cgaagaatgt gatgtgaaaa tatttttaac aggaccactg ccatcaacaa
    20761 aatttccgga aatacgacag gattaaaagc ggcgcacatc aaacaactgg agcgcctcgg
    20821 atataaaggt atcccgccgg aaaacatcat tacgaatgat cttgcccggc aggtctcttc
    20881 gctgtcccgt gaaatcaacc ggcaaatcgg tctgctgatc agccgcaagg gcgaaatcag
    20941 caaagttatt ctcggcgatc acaaaagcat attgattccc tcgctggacg gattccgttc
    21001 ttcttcagtg cgttttaagg gactgcgcct gattcacaca catttaaacg gtgaaaatct
    21061 atccgatgaa gatatgacgg acctgtccca tctgcgcctg gacctcatcg gcgcgctgga
    21121 cgtccgggaa gacggatcac cgggcctgtt gcactgggcg cacctggttc cggaaaatcc
    21181 ggcgggagac tactggctgt tcatgaaacc ggaggaaccg caccagatca aaattaattt
    21241 tctttccttc attcaggcgc tggaggatga gtttgcaaaa aaacagcaat cgcgtaaaat
    21301 tgatgcaacg aataaagcca ttctgctgcg ggttgaaaaa aatccgctgg cgggcgccga
    21361 cgcatcgctg gaagaactgg ctcaactggc gcatacctgc ggtgtggagg ttttcgattc
    21421 catgattcag taccggcccc agcccgatcc caggttcatg gtgggacggg gcaagctgtc
    21481 cgcgatcgat ctgcgcgctt cgcagatcgg cgccaacatg ctcatcttcg atcacgatct
    21541 gacacccgcg caggcgcgtt caatcagcga ctttacgggc ctgaaaatca tcgaccgcac
    21601 acagctcatt ctggatattt ttgcccggcg cgcccacagc cgggaaggga aaattcaggt
    21661 ggaactggca cagctcaaat accggctgcc ccggctgacg catgccgaca cgtcgctgtc
    21721 gcggctggcc ggcggcatcg gcggcaccgg tcccggggaa accaaactgg aaatcgaccg
    21781 ccgccgcgtc cgcgagcgca ttcaccgtct ggaaaaggat ctcaaatcaa tcaccaaatc
    21841 ccggcgtcag cgctatggtc gccgggaaaa aacaggattg ccgattattt ccatcgtcgg
    21901 ttataccaac gcgggcaagt ccacattgct caacacgctg acgcacagtt ccgtcctggc
    21961 ggaagacaaa ctgtttgcga cactcgacac gaaaagcgcg cgtctgcgtt ttccccggga
    22021 cacggaagcc atcatcaccg acacggtggg atttatccgc gatcttccgg aagaattatt
    22081 caccgccttc cgcgcgacgc ttgacgaact gttcgaggcg gatatcctgc tgcatgtcat
    22141 tgacgccagc aacgctcagt tcgaggatca gattgcggcg gtggaaaaaa ttcttacgga
    22201 tctggaaatc cacgacaggc cgacgatccg ggtgctgaac aaatccgacc gtgttgccga
    22261 caaggagctg ctgaaacatc tctgccgcag gctggatgcg gttgccgtct cggctctgga
    22321 taaaaaaacg cttttcgcgc tgatggaaaa aatagaatct gttcttcccc gtaaagacga
    22381 cttttaactt tgccgatttt gtgttattgg aatcccgtgc aaatgatttt ttgacttttg
    22441 cgtttttttg agtattttgt atatatgaaa tgtccggcat ggcaaagacg gccggagcct
    22501 ggttgcagcc tcccccataa tgccagaaaa gcttgtcctc caagctcaaa cgctgaaagg
    22561 caaccccgga acgtaaggaa tatcgcatga acagggattt tattgaaatc cgctggcacg
    22621 gacgcggcgg acagggcgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    22741 agttcaatct ggaatactgc aagggctgcg gcatctgcgc cgaagaatgt ccgaccaaag
    22801 ccatcacgat ggtggacgaa gcacatttct cagaacaaaa aaaggacaag gagtagccgc
    22861 catgtacatg atcgaaaacg gaaataccgc ggcggctctc ggcgtcaaac tctgccgtgc
    22921 cggtgtcatc gcagcttatc ccatcacacc gcagacgccg ctgacggaaa agctctccga
    22981 atatgtggaa ctcggagaaa tgaaggctga atatattccg gtggaaagcg aacactccgc
    23041 gctggcggtg tgcatcgcgg catcatccgc cggcgcgcgc gtttttaccg ccaccagcgc
    23101 caacggcctt ttgtacatgc acgaacagat tcagtgggca tccggcgcga gactgcccat
    23161 cgtgatgtgt gtggtcaacc gcgcggtagg cgctccgtgg aatgtctgga acgaccagca
    23221 ggattccatc tctcagcgcg acaccggctg gattcagatc tactgctgtg atcatcagca
    23281 gattatcgac acggtaatca aagcctactg gctggcggaa aaaataagca ttccgatcat
    23341 ggtctgctac gacggatata ttttatccca cacctacatg ccgtttgatc tgcccgagca
    23401 ggaaaaggtc gatgccttcc tgccgccgtt tcaaccggac tacgccttaa atcccgacga
    23461 accggccaac ctgaacaccg tcaccctgcc ggatgtccgg atcgacacca aaggccagtt
    23521 ggctcacggt tatatggaaa tccggtatct gctgcaggag gaattgagaa caacgctgca
    23581 aatcgcagcc gatgcagaaa agcgatttgc cgaaatattc gggcggggcg ggaatccgta
    23641 tatagaaccc taccgctgcg acgatgccga ttatgtcgcc gtcggcctgg gatccatcac
    23701 ctatcaattg cgcgtctgcg tggacacatt gagaaaagag ggcataaaag tcggcgtgat
    23761 gggcgttcgt tattaccgtc cttttcccga tgccgttatt gccggcgcat taaaagggaa
    23821 gaagggaatc atcgtttttg aaaaggcgct cagctacggc tacgagggcg cgctggcgtc
    23881 ggatctgaaa tcggcgcttt atgaacatct ggcgggaacc ggttccctgc cggccgtgca
    23941 gaatttgatt gccggcatcg gcggccgcga aattcatacc gatgatttga cacaaaatct
    24001 cagagcggca acacagggca ggatttcccg ggaaccggcg tggattggtc tgaagattta
    24061 gcacatccgc aataacaaac gcatcccggt gatggaggtc aggagaggca aatgcaggcg
    24121 aagacaaaca ttttaaattt accgactcag gaatttgttc atccgggcac acgggcctgc
    24181 acgggttgcg gactttccat tgcctaccgg gtcggactca aggccctcgg gaaggatacg
    24241 attctggtcg taccgcccag ttgcctgacc gttttgcagg ggcttttccc cgtggcgtcc
    24301 acaaaactgc cctgcctgaa tgtgacgttc gcctccacgg cggcggcggc taccggtatc
    24361 ctgtcaacgc tgaaaaccaa agggaacgat cacaccaccg ttgctgcctg ggcgggcgac
    24421 ggcggaacca gcgacatcgg actgcaggcc ttatcgggcg ctgcggaacg cggcgataat
    24481 ttcatttatt tctgctacga caatgaaggc tacatgaaca ccggcgtgca gcgctcgggc
    24541 acaacaccga tcggcgcggt caccgccaac acgccgttca agggcaagct gcaacagaaa
    24601 aaagatgttc cggcgatcat ggccgcacat aaactcagct acgtggccgc ctgttcggcc
    24661 gcttatcccc tggatctcta cgacaagatc aaaagctgtc tccatctgcc gggcaccaaa
    24721 tattttcaca ttcatatccc ctgcccgccc ggctggggct atgatccgcg ttacggtatc
    24781 aagatcggcc gtctggctgt ggaaaccggc tattatgatc tctacgagat cgtcaacggc
    24841 gaattccgat taaccgccgc atccgaaaag ctgatcgaaa aacgcaaact gacccccgtg
    24901 cgcgaatatt tccgggcgca gtcgcgcttc cggattctta ccgacgagca gatcaatgag
    24961 attcagcgcc agatcgacat gaaatgggac ggttattata aaaaaggcga gtaaccggac
    25021 ggttgcccga cgcaaccgaa aatgacggcg ggagcaacat gtctcccgcc gtcattttta
    25081 ttattccctg taaagacttt ccagcatttc accgtactta tccataacca cttttcgctt
    25141 gagtttcatg gtctgggtga gcattccgtt atccagtgtg aaatcttctt tgataaaggc
    25201 aaacttgcgc ggaacctcgt aagagccgat gattttcttg agatgatttt gtacttcaat
    25261 ggtcagcaga tccgcgagtt tccggcctgc gggacttgac gggtcaaaaa tggcctgcgg
    25321 atcaaccgat aaatccattt cttccgccac ccgtttgagc accgccatgt ccggcacgat
    25381 cagtcctaca ttgaactcat gtccggcgcc ggtgacaacc gcgttgagaa cccagggcag
    25441 tattttcatt tcccgctcga tggtgtccgg gtgaatatac ttgccgttgg acagtttgta
    25501 ttcttccttg aatcgtccgg taatgtacag aaagccgtca tcatcgagtc gtccccggtc
    25561 gccggtgcgc acgccacgca tgccgtcacg ttcgacgatg acctcctggg tctgttccgg
    25621 ttttttgtgg tagccgatca tgcactgcgg accgaagcag atgatttccc cgtcgtcgcc
    25681 gtcctctccg gtacgcgaac ggtcaatcac gacttttgtt ttgttgatgg gtttgccgac
    25741 cgatcccaaa cgattgccca tcagaggaga attgatcgtg atggccggcg atgtttcgct
    25801 taatccgtag gcgtcatatg tcggaatccc gacatcaata aagaatttgg cgatatcgac
    25861 attcatcggc gcactgccgg tcactgcgtt ttccaggcgg cctccgaaac gctcccggat
    25921 ttttttcaaa actattttat ccagaatttt atatttccaa ccggtctccc cggtctctct
    25981 tttccttttg gccgcctcca gggccgcttc gaacagtttc agtttcaagc cgccttcctc
    26041 ccgcatttta gcccagacac cgttatagac tctgttgaaa atgcggggca cggtgatcat
    26101 gaacgtgggc tgaattttgg gcagatcgtc aatcagcgtt tccaccgatc ccatgatgcc
    26161 gatggatgcg ccctggagaa taaacgcatg caggtcggcc gtgatgccga acgagtgcgc
    26221 ccacggcagc atggaaatgc accggctctg ctgcgtcaga ttgggaaacc aggccaggcc
    26281 ggccttgacg ttttccgcca ggttgccgtg agacaaaaga acgcctttcg gctctccggt
    26341 cgtgccggag gtgtaaatca gcacggcgac atcggagtac ttcggtttgc gtgacggtac
    26401 cggatttgcc gcgcctttcc tctccagggc ggccattgtt ttttctcctt cgccttcaat
    26461 cagatagatg tccttcaacg aggggatttc cgcaggaaaa tctttgactt tgtccagaat
    26521 ctcagacctc gacaccagga gcaccttgat ttcggcatct ttcacaatat atttccacat
    26581 cgagacgagt tctttttcat acatcggcac ataagcggcg cccagcccct gcgtggcgat
    26641 ttctccgatt aaccattcct tgcgattgtt ggcaatgata ccgaccgtgt ctttggcgcc
    26701 cacgccgatg gacgccaatc cggccctcaa atgatccacc ctccgggcaa tatcaccgta
    26761 agtgacccat tgataaactc cggcgctgtc tttttctccg atgagcggat tattcgtgta
    26821 tttcgccgca ctggactcaa aaaagtccac caggttatcc ggtggttcat acacattact
    26881 gtattcgcca tattcaaaga tcgccataaa cctattcctc cctttcggtt taaatcctga
    26941 actgatacaa atccggtacg ttatgggttg gggttgtctg gtaaacgttt aaaacgaagt
    27001 ttcaatagaa agacgatcgc aaaaactctg ttgtcgtaaa aggatagcgc ccaaaagatt
    27061 gccgacactc tttgatcggc tttgcgtttt ggataagtta attatacggg caatgactcg
    27121 ccaagtcaag ccaattcgaa tacctcgaat tggccgttcc agaggtgcat cagccttgaa
    27181 gcgcctgttt caaaatttcc atattcat
//
LOCUS       DAONQZ010000027        26588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_1993_length_26588_cov_18.613877, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000027 DAONQZ010000000
VERSION     DAONQZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 26588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26588
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_26588_cov_18.613877"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /gene="gyrB"
                     /locus_tag="PLR13_06175"
     CDS             complement(<1..1977)
                     /gene="gyrB"
                     /locus_tag="PLR13_06175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQC18958.1"
                     /translation="MTHNYDADSIKVLEGLEAVRKRPAMYIGSTGKEGLHHLVYEVVD
                     NSIDEAVGGFCDTITVKIRVDNSIVVDDNGRGIPIDMHKTEGVSAAEVVMTKLHAGGK
                     FSNDSYKISGGLHGVGVSVVNALSKTCELEVRRDGNVYMQSYKRGIPDAPLEKIGKTR
                     GKGTKVTFLPDDEIFEETEFSYDIIANRLRELAFLNSGVKIILIDERANKQNEFFYKG
                     GIISFVEYINRNKKVLHKNPIYVSGAREDCSVEVALQYNETYLENIFSFANSINTTEG
                     GTHMIGFRSALTRVFNNYATNNKILKDGKESLRGEDLREGLACVISVKVKNPQFEGQT
                     KTKLGNSEVRGLVEGIIYDKIGSYLEENPSVAKQILNKCMEAARAREAARRARELTRR
                     KSALEVGALPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKTMVTAFGAGIGADDYDISKLRYHKVIIMTDADVDGAHIRTLL
                     LTFFFRQMRELIEKGYLFIAQPPLFKVVEKKQEFYLQNEEQMKNYILENGVGKVKLMM
                     GDGSPFPVNGSKLLTLIKKVMRIRDLMERFEKEGRDGNLIRILAGDPTLTDDSFKSEK
                     ALAAVAKRTGMALGESFADFQIETDEDHGGFKIVF"
     gene            complement(2005..3114)
                     /gene="dnaN"
                     /locus_tag="PLR13_06180"
     CDS             complement(2005..3114)
                     /gene="dnaN"
                     /locus_tag="PLR13_06180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQC18959.1"
                     /translation="MEFKINREIFLDGIQKTLGIVEKKTTIPILNNILIKAEAGKIKI
                     IATDREIILISDYEAEVMEKGEITISAKKIYEMIREIQGEFINFIQKNNLVKISAQRA
                     FYKIPGLPADDFPSIADDQDIPFSKIQGSIIKDLINKTSFAMATDETRKNLNGVLMEA
                     GLDGSNYLLRMVATDGHRLAMARGFTPEPTLKTVKGIIIPRKGLMEIKKIIDENKEVE
                     IGLHKNMFLVKTENTLLKVSLVDADYPDYKKVIPTEKGMSVVLEKDYFLHALRRMSVV
                     SSERYGGVILSFSKGKLTLNSTNLDVGEATEEIDIDYGGEAIDSGFNVNYLIDAIAVV
                     NKENIVFEVGLGLKPSIIKQAEDDNYLCIIMPLKI"
     gene            complement(3266..4639)
                     /gene="dnaA"
                     /locus_tag="PLR13_06185"
     CDS             complement(3266..4639)
                     /gene="dnaA"
                     /locus_tag="PLR13_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQC18960.1"
                     /translation="MFVDKGFKMDVFWDKTSKIIQEKISKQNFDTWIKPIQILAMEEQ
                     CVKLAVPNKFFRDWLVDNYSSTIKQSLKDASGLDVNIDFILSKTKETDHEPKAKQTAA
                     AKTAAPAVHSRSKTFSFLNDNYSFERFVVGSCNQFAHAASVAVAKQPAKNYNPLFIYG
                     GSGLGKTHLLNAIGLMANSIHPSLNIMYVSAEEFMNEMINSIRYDRMPKFREKYRNIS
                     CLLIDDIHFLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEHRLRSRFEW
                     GLIADIQPPEIETKIAIIERKIQENKIELPNNVIHYIASHVESNIRELEGFLVRIGAY
                     SSLTGREIDLDLVKEVLSNLVKHNHKGEISIEEIMKVISGKLNIKISDIKAHNKNKNI
                     VFARQIVMYLARKLTNQSFPDIGQKIGGRDHSTVIYANNKIANNIKSDIKLKNLIQEI
                     EDILIHK"
     gene            complement(4674..5531)
                     /locus_tag="PLR13_06190"
     CDS             complement(4674..5531)
                     /locus_tag="PLR13_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQC18961.1"
                     /translation="MAQKNVLGKGLGAIFPDLINNESDKKKSLYCGIEELHPNRYQPR
                     KTFHDDKQKELIASIKSSGIIQPIIVRKTNSGYEIIAGERRWRAAQAAGLKDVPIVIR
                     EASDREAAELSLIENIQREELNPIEEAGAYVTLMDQFKLSQEDISARVGKDRSTISNT
                     IRLLKLPAKIQSALMANQISAGHARCLLACPSPEEQIAALTLILKKDLNVRETERLIQ
                     RAKKIPLDKKQPVKKDRFLNDLEKAMTLKLQAKVQINGSDKKGLIEIRYTSMDELNRL
                     SGLILDDLQ"
     gene            complement(5532..6299)
                     /locus_tag="PLR13_06195"
     CDS             complement(5532..6299)
                     /locus_tag="PLR13_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18962.1"
                     /translation="MNKIICIANQKGGVGKTTTAINLSASLAAAEKKTLLIDGDSQGN
                     STSGVGVDRAVLETANLYHAMIGDVPLKDVIMHTAIPHLDVVPSTQDLIGIEVEFVGL
                     EDKEKRLKQLLNDLDARYDFIVIDCPPSLGVMTVNALVAADYLIVPLQCEYYAMEGLG
                     YLLNTVKLVKAQLNPHLVLGGILLTMFDQRNLLSHQVSDDVRKHFGDKVFKTVIPRNV
                     RLSESPSHGLPVILYDIKSRGAMAYMEMAKEIIQGGL"
     gene            complement(6384..7121)
                     /gene="rsmG"
                     /locus_tag="PLR13_06200"
     CDS             complement(6384..7121)
                     /gene="rsmG"
                     /locus_tag="PLR13_06200"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQC18963.1"
                     /translation="MTRWTPMTGHEYNLKFAAVDLFCYCTGMNHDIAFLKNQARQLNI
                     ALSNGQLQQFDVYKNELMDWNAKTNLISEKSARDILARHFLDSLTALRYITPPDARII
                     DVGSGAGFPGLPLKIAQPSLDLYLLEANRKKVSFLKHIVRLLNLSSVDIIHDRVENII
                     RENVYQEKFDVLISRASFKLPQLCSFCRYFLTPAGRLIALKGPGVEAEIQKCFEDRNQ
                     SGIYQLIQQPIEAPFLNSTRKIIIGQR"
     gene            7232..7906
                     /locus_tag="PLR13_06205"
     CDS             7232..7906
                     /locus_tag="PLR13_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC18964.1"
                     /translation="MKKIIICFFLCGTLMLTGCAQTGTMTKSQTGGLVGATGGAVAGA
                     VLGQVIGRDTKATLIGAAIGAAIGGAGGYGVGTMMDNQERDMRDALATSEAAAVTREG
                     NLLSVTFKGDVTFDTNSAEVRPGLYSEINRVAAILNQYPQTLIQIEGHTDSVGSDDYN
                     MKLSQRRANTVKTLLVQRGVAASRIETIGYGETMPVATNNTEVGRQMNRRVEIKIAPQ
                     TQAQTP"
     gene            complement(8045..8218)
                     /locus_tag="PLR13_06210"
     CDS             complement(8045..8218)
                     /locus_tag="PLR13_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18965.1"
                     /translation="MSKLNLTQEEENELLLILERYLPDIQMEIANTDSKEFRRELKER
                     EVFMNDLIARLKR"
     gene            complement(8629..9345)
                     /locus_tag="PLR13_06215"
     CDS             complement(8629..9345)
                     /locus_tag="PLR13_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18966.1"
                     /translation="MNSSTNGWDFFDRFYCISLRKREDRRQSARKEFEKVGLANRVEF
                     VIGERLSDDFEREVYEAHMLCLRKGLEAGAKNIVVFEDDVEFDRFDPERLQKCTDFLR
                     RHPGWKVLLLGALIRSSRNTDDPCVQKVRYQSLTHAYALNAPYAKTLAYDPWSGIVND
                     TLFRPLTEDVYAVCPMCAFQKNFTSDNYKYPRLALIRRLFGGLKRIQKANEFYQRYKV
                     GVIAAHLAVVILILFLLWRG"
     gene            9516..9881
                     /locus_tag="PLR13_06220"
     CDS             9516..9881
                     /locus_tag="PLR13_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18967.1"
                     /translation="MIKKNLGQYLAQKKFIRKILDDPVDLSEFKQRPTPRLIGGLVLM
                     ALSYVIGWPIIFVLSILAVSFHEPLIFVIGGPLAYAVSCVVFLIGAWMARAPHYMNTL
                     TRYALQSLLKKLFDPVRTD"
     gene            complement(9982..>10877)
                     /locus_tag="PLR13_06225"
     CDS             complement(9982..>10877)
                     /locus_tag="PLR13_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family murein peptide amidase A"
                     /protein_id="HQC18968.1"
                     /translation="ISVGLVLLFAFFLLLPLQSFAYDCSTREGKQYLRLLERKFADYR
                     WTDLHPRDISWEYHRTTRNKHPLMFVHFGNSSKNCVLFFGGVHGDELPTAYLMFKLAH
                     YVKEHPALFKDQCIVIAPLLNPDGFLSAQPTRTNASGIDINRNFPTRDWKAKAIRQWI
                     AKGKQVRYYPGTRPASEQETIFQMALIKRFKPQKILSAHSPLNFFDYDGPSSDLDSFE
                     KWMEQICKESNHPLKKFGYYPGSLGNYAGHERNIFTLTLELPSSDPGQSSDYFEKFQP
                     SIVKFIQLPIVGSPPNLKVLR"
     assembly_gap    10878..10977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11326..12066
                     /locus_tag="PLR13_06230"
     CDS             11326..12066
                     /locus_tag="PLR13_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQC18969.1"
                     /translation="MLKTQLKNISVVLYKPKYAGNIGAVARAAKNMGIRKMIVVGTDD
                     FDREEMRQRSTHLAADVLDDIQYIENAAEAVSPFNYIVGTTARTGKARGPFVTPRQAA
                     QTIADISQKNKVALLFGPEDTGLANEQLRLCHMVVTIPTSREFTSLNLSHAVMILCYE
                     IFTASCIAVTASEATPKLALSSELEGMYGQIKTLLADIGFLNPENPDYWMMHLRRFFT
                     RAGILSREVKIVRGICRQLQWYAHHKKT"
     gene            12202..13359
                     /locus_tag="PLR13_06235"
     CDS             12202..13359
                     /locus_tag="PLR13_06235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC18970.1"
                     /translation="MRKTFSFTGAKKIVFGSGSLLTLASHVKEHHARNPLVVIDKNLA
                     KTDLLEKVANVLIPSGIKFTVYEKVEAEPPIELADEGAKAAMKNKCDIVIGIGGGSAM
                     DVAKAIAVIATNKGAAADYLGLNKVPKPGLPKIMIPTTAGTGSEVTFTAVFLRKNLKK
                     KEGMNSPYLYPELALLDPELTLSLPPVPTAHTGLDALCHAIESYTSINSSPVSEMFSL
                     EAIALIAENLRTCVHDGKNIVARERMLLGSLYAGIGLANAGVTAVHSLSYPLGGKYGV
                     GHGLANTMLLPAVMAFNMPSALEKFTDIAEAMGENVEGLPEREAAYLALEAVEALIED
                     CGIHDTISTFGVKEKDFPALADVAVTVARPLENNPRKLTKDDMIAIYTQAF"
     gene            13377..14579
                     /locus_tag="PLR13_06240"
     CDS             13377..14579
                     /locus_tag="PLR13_06240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQC18971.1"
                     /translation="MAGAELIGKEEIKEVMDVLETGILMRYGFDKERKGIYKVREFEQ
                     AFAKYCGTDYALGVTSGSSALQVALTAMDVGPGDDVLVPAFTFLATYEAVMEVGAIPI
                     MVDIDDTLNFDPLEIEKKKTPYTKAVIPVHMCGSAANIDKIVDVARKNKLLVLEDNAQ
                     GCGASYKGKKLGSFGDMGTFSFDYVKTVTTGEGGMVITNNKEFYLRSEWYHDHGHDHN
                     PKVSRALEGRTILGFNFRMNELQGALGLAQLRKLDYLIGEQKKNKKFIMDVLANVSGV
                     KFRVKPDPEGDSATFLAFNLPSEDQAQKFQNLLAAEGVDTVCYKRNLWHYVPNWEHFL
                     AKSTANSKKYPLTNPAYKGKVEYRKENIPQAEDILGRTLVMGISVKMSQEKLDGIRKG
                     IEQAAKNM"
     gene            complement(14746..>14978)
                     /locus_tag="PLR13_06245"
     CDS             complement(14746..>14978)
                     /locus_tag="PLR13_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HQC18972.1"
                     /translation="ILKWAGGHPGGTLAMGQAVNRDFSTEFENLYVCDGSTMPVSPGA
                     PPSLSICSMSLLLGKMLLGKVKAQDRLIKAAG"
     assembly_gap    14979..15078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15079..16271)
                     /locus_tag="PLR13_06250"
     CDS             complement(<15079..16271)
                     /locus_tag="PLR13_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQC18973.1"
                     /translation="MKKKGSGYEYIIVGTGPGGAPAARELAKAGKKVLMVEKGDYHKK
                     FLGLPFGIRLLERYMLFARSREGVIIERGITVGGSSMVYQSNVMDPPVKLINNMGVDF
                     RPEAKQLKKEIGIEVLPEKFYRNNKGLYRMLATADKMGIPFREQEKFVHADKCKLGCD
                     WCMLGCPENARWTTREHVEEAKSYGAELIFNAPVNKILFNAGKTKATGIQLKNGTVIH
                     GDNIILSAGGIGSPALLLRSGVKRLGTQDVGTRFFMDPMNIMLGYSKDPDGGLWGIQT
                     FSHAIESMAQSEGFMIGNCSAFGTWTVLSFFRMQTARENLPKFPIMKRGMGLFLKLSD
                     EAHGQIFPNEKTSKPFTANDEKRLKKGTDIMREIIIKSGGDPKTISILKWAGGHPGGT
                     LAMGQA"
     gene            complement(16290..17480)
                     /locus_tag="PLR13_06255"
     CDS             complement(16290..17480)
                     /locus_tag="PLR13_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HQC18974.1"
                     /translation="MKTILDETRELASSTHNPYLEEARKQGKKIIGYFCSYMPEEIIH
                     AAGFVPYRMRAVESKGTTRADAFYSSINCTFVKHCFEKALKGDFAFLDGVVFVNGCDH
                     SRRMYDNWRFAKIDPPFLHMFFAPHMINEAALDYFSLECSKLKSSIEKHFHISINNES
                     LRHSINLYNQKRRLLAQIYALRKMKDVPIKASELLGVMLAVTAVPVERAIDILSNVKN
                     YITGRVVSKPQDLRLFFSGGCIEEMDHLELIENCGAVIVADNICLGSRHFLDEIKNDG
                     NPLQAIADRYLSHLSCPRMMGDFERRFNYLLDVRDEYGIDGIIIEKLKFCDIWGGEIY
                     LHRTESKKRGLPLLALERELYGGGIGQIKTRVQAFLEKLRNKQATDENLVQAAGADYK
                     PKIQ"
     gene            complement(17477..18868)
                     /locus_tag="PLR13_06260"
     CDS             complement(17477..18868)
                     /locus_tag="PLR13_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HQC18975.1"
                     /translation="MKNNPKPFQYLKWSSSFLNFGYYLTTFPNLSYWALRLTRPLYVK
                     RETFFNHKFRYAFLILGGGILPPEEIKAYHYGAKAFVDNMKNALKANENGKPVVWVEW
                     ILSSEIVNAFDAFPFNPETLNVFGNLSGQDVPSLLIEEAESAGFASEYCSAIKLTAGA
                     YMLGQTPKPAAIIAGSHPCDTNVAAYQGIRHLTGAPVFFFDSPYWRDKDAFDYYEKNM
                     WRLIDFLEKTLHQKINWERLKQIVDHENKFNFYMQEICEMMRAIPSPATMITPVFAWV
                     VRELAIGDPNGTEMARRMYEVVKDRFKKGVGVVKKENIRVLWWNPPIAFFAYVFKWME
                     DEFGAVVVKDFIGDVQMPQIDTSSEKTMIRDMAKNHLYLAMARQCHGPVEFITQELEK
                     AIDEYSPDCLIFAGHAGCKHGWAVIGIIKDLLKKRGLPSLFMNMDIMDQRHATEDDIK
                     RWITEFFRSNGFV"
     gene            complement(18891..19241)
                     /locus_tag="PLR13_06265"
     CDS             complement(18891..19241)
                     /locus_tag="PLR13_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC18976.1"
                     /translation="MLDLSLGKKGKIPNYFKEAQMTYGVVVFDPTLCTGCGICVSICP
                     ARGLMLTKRQDSAKKKIPLVIETAPGISLCMACGDCTAACPEAAIRIKRGFNAGFFFR
                     KLSQQPELTLPKKY"
     gene            complement(19243..19992)
                     /locus_tag="PLR13_06270"
     CDS             complement(19243..19992)
                     /locus_tag="PLR13_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQC18977.1"
                     /translation="MAKQTTDNLSPLYTYQEGMEWNPVEKVIMERRSIRNFKKEPVPD
                     NLIRRVLEAGRFAPTAGNAQPWKFIVVKDPALISEMERATIQLSKLLMWFVDYNRNAF
                     RRIFLAPYTKFMTRLMPNKLHPIPFNLLQQIAAEKTAVYYNAPVMILLLIDKRGIGNP
                     VLDAGICGQNIVLAAHSLGLGTCWIGMMTVLMMNPLWRRKLGVKSPYELTDCIVLGWP
                     KGDYDGEVTREVQLVEWLEAGGEKRIERQGV"
     gene            complement(20027..20476)
                     /locus_tag="PLR13_06275"
     CDS             complement(20027..20476)
                     /locus_tag="PLR13_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18978.1"
                     /translation="MSKINTGFWQKMFFVGSLWNLGIGITSLLFTDFMLMMMFGKGPI
                     EDNLLAFINGTVPVTDNLQTLIFFRFFMIAVILFGIGYYWVSRDLLANRAVIWLGLVA
                     KLIIFFTFVYYYALEQAAWFPVFVLSGDFVFSIFFVAFLWKTKDGIY"
     gene            complement(20541..22241)
                     /locus_tag="PLR13_06280"
     CDS             complement(20541..22241)
                     /locus_tag="PLR13_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC18979.1"
                     /translation="MNRKLRKLDLGKNILKSICLFSINEIRRGSFLKNIRGVSRTEIR
                     QEMSWGELLEEKAARYKDKIFLIFEDKTFTYRQMNANANRIANYFLSSGAGQGKGVAI
                     LMDNSPSFIDIFIGIQKIGMYAVPVNTSLKGDSLLYILNHCDAEYLVIDETHLETLNK
                     IADRLDKIKTIIINPSPSSSRQYPAGYHVLDEAYKGSPANPGLGYRKDDICFITYTSG
                     TTGLPKGVVYRYRKSSVKLLSLVAVMLFKKSDVLYTGMPLFHGNALFVTVTCALHTGA
                     RVVLARKFSASRFWDDIRKYNVTTFNTIGAMIPILMKQPEKPSDRQHNVRFIISAACP
                     ADLWEKFEQRFGLTIYETYGAVDGGGKIILNLGTAPVGSIGKPPMKVKYRLVDANMKD
                     VPDGTPGELVFPVSNKKSTSSVEYYKNEKASAEKMNGDYIFTGDLIRRDTEGFLYFIG
                     RNTESMRIKGENVSAFEVEQAIQKHSNVLEAAVYAVPSELAEDDIMASITLVEGVTLK
                     ETELIEYLKENLPKFAVPRYVKIVPELPKTETHRVKKKELEALKVVTGTWDENKKAYV
                     "
     gene            complement(22264..23667)
                     /locus_tag="PLR13_06285"
     CDS             complement(22264..23667)
                     /locus_tag="PLR13_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC18980.1"
                     /translation="MRDVYVVAAHTIKFGKYLEKTISELSAMTVSPCLKEAGLDKKDI
                     QALWFSNSAWGEYKRQVCIRGQVALRPLGIDAIPITNLENACAGGSTAFHHAWMGVAS
                     GMYDITMALGAEKIYDQNQALVFSGFLTGLDVGNFGKTLERLGDVALSENEKKAMATH
                     IEKYKGLAKQSSGKGRKKSFKDKFMAQRDALVVAIRIGETMGYDMLKKLRKLGSGDHS
                     PFMDVYGYEARQHMKKFGTTIEHLAAIASKNHFHSSLNPNAQYQFLVSTEQVLADRLV
                     TWPLTRSMCAPIGDGAASAILCDEATVRRLGLSGQAVKVRASILGSGMARDRDGIDIG
                     ERLSRLAYEKAGVGPAEIAFAEVHDATAYGELHQCESLGFCRLGEGGRYAESGATKLG
                     GKQPVNPSGGLESRGHPIGASGLAQIHELVTQLRGAAGPRQVSGAKLALAENGGGALG
                     VEEAAMCIHILEAPSLK"
     gene            complement(23727..24521)
                     /locus_tag="PLR13_06290"
     CDS             complement(23727..24521)
                     /locus_tag="PLR13_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC18981.1"
                     /translation="MNEPIILAFLGKGGVGKTVLSALTGTILSACGKKVLFVDADPAM
                     GLTSALEVSDIKTIGAAREEIIRQARISSSSEEKEKLADIVDYLLMEALYEGQGFGMV
                     AMGQTNTLGCYCPVNNLLRSTIKSIASQYEAVIIDAEAGIEQVNRQVVESVHYPVIVT
                     DNSLRGARTAVMISSLLKKIPQLSPVKVGTVFNRVDQADENLVKMILEAGMDYYGSLR
                     PDNAISALDRQGLSIAKIAGTSPAAAGMRSVLEQIGAFGKILNVKK"
     gene            complement(24524..>24933)
                     /locus_tag="PLR13_06295"
     CDS             complement(24524..>24933)
                     /locus_tag="PLR13_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18982.1"
                     /translation="DFDPTGPNIQTLSNLTNRIPGYMTRSIPGKIWIRIHRDLLARKF
                     SLQSLGQCLIQAYHESIPGLKNIEVALIADNADFVGHLIPIHNRARIIYGENKKLVLE
                     ADGTISCEDLDCENCTEKPTCDTLRDVIVKRRT"
     assembly_gap    24934..25033
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25034..25782)
                     /locus_tag="PLR13_06300"
     CDS             complement(<25034..25782)
                     /locus_tag="PLR13_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HQC18983.1"
                     /translation="MITAGVDVGTRFIKVCVTDGEHLRGSACAEIGPRFDALIRSLLK
                     EARVQARAGRWEIKKIVATGFGGHLVKKAAFTLGEAACTARSAFFFDRDIRTVIDLGG
                     LFITVSTIDENGFLETTHVNERCAAGSGKFLEMVAEAAEVPFTEISSCAAASRQPYSS
                     SSSCAVFAESEIISQVNTGMKPSDILAGVIRAIASKTASLIDGAGAKDKIVLIGGLSK
                     VSAVRDMLTGLTGRSMTTLPIDSQLVAAFGAA"
     gene            complement(25779..26570)
                     /locus_tag="PLR13_06305"
     CDS             complement(25779..26570)
                     /locus_tag="PLR13_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HQC18984.1"
                     /translation="MIVAGCDVGSLTAKALIMNEKGRLAGEITRVRLSTLDSATEVME
                     KALASAKLRFSDLDYCCSTGYGRYEIPFAQMNMSEISCHGLGAFRADRSVRTIIDIGG
                     QDCKIILIDAEGNVRDFIMNDKCAAGTGRSLEMLAKTIGVDLETLGSLAVKSKNPISI
                     TNKCSIFMELEVLNHLYARKKLKHIACGIADAVAKRVAALAKAIDVEKNICITGGVSK
                     NPGVVRQLAYHMNVLFTSLPVDPQIIGAMGAAVYAMETLKAKVGM"
BASE COUNT         6519 a         6483 c         6221 g         7065 t
ORIGIN      
        1 gaaaacgatt ttaaagccgc cgtgatcttc atcggtttcg atttgaaaat cggcaaaact
       61 ctcgcccagc gccatgccgg ttcttttggc gacggccgca agcgcttttt cgctcttaaa
      121 actgtcatcg gtcagggttg ggtcgccggc cagaatacgg atcagattgc cgtcccggcc
      181 ttccttttca aaccgttcca tcaggtctct aatccgcatg acttttttaa tcagggtgag
      241 cagcttgctg ccgttgaccg gaaacggcga gccgtcgccc atcatcagct tgaccttgcc
      301 cacgccgttt tccagaatat agtttttcat ctgctcttca ttctgcagat agaattcctg
      361 ctttttttcc accactttaa acagcggcgg ctgcgcaata aacaaatacc ctttttcaat
      421 caactcccgc atctgccgga agaaaaaggt cagaagcagc gtgcgaatat gcgcgccgtc
      481 cacatccgca tccgtcatga tgatgacttt atgataccgc aatttactga tgtcgtaatc
      541 gtcggcgccg ataccggctc cgaaagccgt caccattgtt ttgatttcct cattctgcag
      601 cattttatcg aaacgcgctt tctctacgtt tagaacttta ccgcgcaacg gcaatatcgc
      661 ctgattctta cggtcccggc cctgcttggc cgaaccgccg gcggaatcgc cctccaccag
      721 atagatttcg ctaagcgccg gatccttttc ctggcaatcg gccagtttgc ccggcagcgc
      781 gccgacttcc aaggcgcttt tacggcgggt cagttcccgg gcccgtctgg ccgcttcacg
      841 ggcccgagcc gcttccatac acttgttgag aatctgtttg gccacggagg ggttttcttc
      901 caggtagctg ccgattttat cgtagatgat gccttccacc aggccgcgga cttcgctgtt
      961 gcccagcttg gttttcgtct gaccttcgaa ctgcggattt ttcaccttga cactgatcac
     1021 acaggccagc ccttcccgaa gatcttcgcc gcgcagcgat tctttgccgt ctttgagaat
     1081 tttattgttg gtcgcgtaat tattgaacac gcgggtcaga gcggaacgaa atccgatcat
     1141 gtgcgttccg ccttcggtgg tgttgatgct gttggcaaat gaaaaaatgt tttccaggta
     1201 cgtttcattg tactgcagcg ctacttccac agagcagtct tcccgtgcgc ctgaaacata
     1261 aatcggattt ttatgcagaa cttttttatt gcgattgatg tattccacaa aggaaatgat
     1321 gcctcctttg tagaaaaatt cattctgctt gttggcacgt tcatcaatga gaataatttt
     1381 aacgcctgaa tttaaaaagg ccaattcgcg caaacgattg gcaataatat cgtagctgaa
     1441 ttccgtttct tcaaagattt catcgtcagg taaaaaagtg actttggtgc ccttcccccg
     1501 ggttttaccg atcttttcca gaggagcatc cggaataccg cgtttatagg attgcatata
     1561 aacattgccg tcacgacgca cttccagctc gcaggttttg gaaagcgcat ttacaacaga
     1621 tacgcccacg ccatggagac ctccggatat tttataggag tcattggaaa atttcccgcc
     1681 ggcgtgcagc ttggtcatga cgacttccgc cgccgacacg ccttccgttt tatgcatatc
     1741 aatcggaata ccgcggccat tgtcatctac gacaatactg ttatccaccc tgattttaac
     1801 ggtgatggta tcacaaaaac cacccactgc ttcatcaata ctattatcga cgacttcata
     1861 aacaagatgg tgaagacctt cttttccggt ggaacctatg tacattgccg gccgcttacg
     1921 aacagcttcc agaccttcta ataccttaat actatccgca tcataattat gtgtcatgtt
     1981 tgattttctg cttcctctct gaaattatat tttcaacggc ataataatgc ataaataatt
     2041 atcatcttcc gcctgcttaa tgatggacgg tttcaatccc aaaccgactt caaaaacaat
     2101 attctcttta ttgacgacag cgatggcatc gattaaatag ttgacattaa atccactatc
     2161 aatcgcctcg ccaccataat cgatatcgat ttcttccgtg gcttctccaa catccagatt
     2221 cgtcgaattc agtgttaatt ttcccttgga aaaagaaaga atcactccgc cgtaccgttc
     2281 ggaagataca acactcattc ttctcaacgc atgcagaaaa tagtcttttt ccagtacaac
     2341 actcatcccc ttttcggttg gaatcacttt tttataatca ggataatcgg cgtccacaag
     2401 actgaccttc agaagggtat tttccgtttt aaccagaaac atgtttttat gcagaccgat
     2461 ctcaacctct ttattctcgt caataatctt tttgatttcc atcaaccctt tgcggggaat
     2521 aataatccct ttgaccgttt ttaacgtcgg ctccggtgta aatcccctgg ccatggccag
     2581 acgatgccca tccgttgcca ccatccgcaa taaataattc gagccatcca gtcccgcttc
     2641 catcaagaca ccgtttaaat tttttcttgt ctcatccgtc gccatggcaa aagacgtctt
     2701 gttgattaga tctttgataa tacttccctg aatcttggaa aacggtatat cctgatcatc
     2761 ggcaatactc gggaaatcat cagcaggcaa tcccggtatt ttataaaaag ctctttgcgc
     2821 ggatatcttc acaagattat tcttctgaat aaaattaata aactctccct gtatttcacg
     2881 gatcatttcg tatatcttct tggcggaaat ggttatttct cccttttcca tcacttccgc
     2941 ttcgtaatcg gaaattaaaa taatctctct atctgtggca ataattttga ttttaccggc
     3001 ttcagcttta ataaggatat tatttaaaat aggaattgtg gtttttttct caacaatacc
     3061 taaagttttc tggatcccat caagaaagat ttctcgattg attttaaatt ccatatagcc
     3121 tccttatttt tagtaattct ttttataaat aaaaaaatag tattaatagt atagtttgtt
     3181 tatatgttga taaatgtttt ttctttaatt aaattcatat tatatttttc ttccgcctgt
     3241 ttataatttt tgtattaaaa gaggattatt tgtggattaa aatatcctct atttcctgaa
     3301 taaggttttt caactttata tccgatttta tattgtttgc tattttattg tttgcataaa
     3361 tcactgtgga gtgatcacga ccgccgattt tctgcccgat gtcgggaaaa gattgattgg
     3421 ttagttttct ggctagatac ataacaatct gacgtgcaaa aacgatattc ttatttttat
     3481 tatgggcttt gatatccgag attttaatat ttaattttcc ggagatgacc ttcataatct
     3541 cttcaatact aatttcccct ttatgattgt gtttcacgag attactcaga acttccttaa
     3601 ctaaatccag gtcgatttcc cggcctgtca gcgaggaata agcgccgata cgcaccagaa
     3661 aaccttccag ttcgcgaatg ttggactcaa catgagacgc gatgtagtga atgacattat
     3721 tgggcagttc aattttattt tcctgaattt tccgttcaat gatggcaatt ttggtttcaa
     3781 tttcaggcgg ttgaatatcg gcaatcaggc cccattcgaa gcgtgaacgc agtctatgtt
     3841 ccaggttggg aatatctttg ggaaatttgt cgctggtgac gacaatttgt tttccggagt
     3901 cgtgcagggt gttgaatgta tggaaaaatt cttcctgggt tctgtctttg cccgccaaaa
     3961 aatgaatgtc atcgatcaac aggcagctga tattccggta tttttcccga aacttgggca
     4021 ttctgtcgta acggatggag ttgatcattt cattcataaa ttcttccgcc gaaacataca
     4081 tgatattgag tgacggatga atggaattgg ccatcagacc gatcgcgttt aataagtgtg
     4141 tttttccaag tcctgacccg ccgtaaataa aaagaggatt gtaatttttg gcgggctgct
     4201 tggcgaccgc aacagaggcg gcatgggcaa attgattaca ggacccgacc acgaatcgtt
     4261 caaaactgta gttatcgttt aaaaaagaaa aggttttact gcggctatgg actgccggag
     4321 cagccgtttt ggcggcagca gtctgttttg ctttgggctc gtgatcggtt tctttggttt
     4381 tggaaagtat aaaatcaata ttcacatcaa gaccggaagc gtccttgagc gattgtttaa
     4441 tggttgagga ataattatcc accagccagt ccctgaaaaa tttattcggt acggccaact
     4501 tgacacactg ctcttccata gccaaaattt gaatgggctt aatccacgtg tcaaaattct
     4561 gcttacttat tttttcctga atgattttac tagttttatc ccaaaacaca tccattttaa
     4621 aacctttatc aacaaacata tccacaactg ttgatatacc taattaacct gatttactgt
     4681 aaatcgtcaa gaattaaacc ggacaagcgg ttcaattcgt ccatagacgt ataccggatt
     4741 tcaatgagcc cttttttgtc cgatccgttg atttggactt tggcctgtaa ttttaacgtc
     4801 atggcttttt ccaaatcatt gagaaaccgg tcttttttta ccggctgttt tttgtccagc
     4861 gggatttttt tcgcccgttg tatcagtcgc tccgtttccc ggacatttaa atcttttttt
     4921 aagatcaggg tcaaagcggc aatctgttct tccggtgaag gacatgccag caaacagcgc
     4981 gcatgaccgg ctgaaatctg attggccatc agagcgcttt gtattttagc cggcaatttt
     5041 aacagccgaa tggtgttgga aatcgtcgac cggtcttttc caacccgtgc ggaaatgtcc
     5101 tcctgtgaaa gcttgaattg gtccatcagg gtcacataag cgccggcttc ttcaatcgga
     5161 tttaattctt cccgctgaat gttttcaatc agcgacagtt ctgccgcttc cctgtcgctg
     5221 gcttcacgga ttacaatagg gacatctttt aaacccgcag cctgagccgc cctccagcgc
     5281 cgttcgccgg cgataatttc gtaaccgcta ttggtcttac ggacgataat cggttgaatg
     5341 atcccgctgc ttttgatcga ggcgatcaat tccttctgtt tatcatcgtg aaaagtttta
     5401 cggggctgat aacgattggg gtggagttct tctatgccgc agtacaaact ttttttctta
     5461 tcggattcat tgttgattaa atcgggaaat atagcgccca agccttttcc cagcacattt
     5521 ttttgtgcca tttacaatcc cccttggatg atttccttgg ccatttccat ataggccatc
     5581 gcgccgcgcg atttaatatc gtaaagaatg acgggcagtc cgtgactggg actttccgag
     5641 agccgcacgt tacgcggaat gaccgtttta aatactttgt caccgaaatg tttgcgcaca
     5701 tcatcggaca cctgatgcga cagcaaattg cgctgatcga acatggtgag cagaatgccg
     5761 cccaaaacca gatggggatt caattgcgcc ttgaccagtt taacggtgtt gagaagatac
     5821 cccagacctt ccatcgcata atattcacat tgtaaaggaa caatcaaata atcagctgcg
     5881 accagagcgt tgactgtcat aacgcccagg gacggcgggc aatcgatgac gataaaatca
     5941 taacgcgcgt ccagatcgtt caataactgt ttcagccgtt tttctttatc ttccagaccg
     6001 acaaattcca cttcgatgcc gatcagatcc tgtgtggacg ggacaacgtc cagatgcgga
     6061 attgccgtgt gcatgatcac gtctttgagc ggcacatcgc cgatcatggc atgatacaaa
     6121 ttggccgtct ccaacaccgc tctgtcaacc ccgacaccgc ttgtggaatt gccctgagag
     6181 tcgccgtcaa tcagcaacgt ttttttttcc gcggcagcca gagaggcgga taaatttatt
     6241 gcagtcgtag ttttcccgac accacccttc tggttcgcta tgcatatgat tttattcatt
     6301 tattatttag tatcgcaaac aatgttcttt agcaggaact gtggatagca ctagcataaa
     6361 aaaatgcttt ttagaaggat tttttatctt tgcccgataa taattttgcg cgttgaattc
     6421 aaaaaaggag cctctatagg ctgttgaatc aattgataaa ttccggattg attccggtcc
     6481 tcaaaacatt tttgaatttc cgcttccacc ccgggacctt ttaaggcaat caaccggcct
     6541 gcgggggtga gaaaataccg gcagaatgaa cacagttgcg gcaatttgaa cgaggcccgg
     6601 gaaatcagaa cgtcaaattt ttcctgataa acattttccc ggatgatgtt ttcaacgcgg
     6661 tcatgaataa tatcgactga agaaagatta agcagccgca cgatgtgttt taaaaaggac
     6721 actttcttgc gattggcttc cagaagataa aggtccagcg aaggctgcgc tatttttaag
     6781 ggaagaccgg gaaacccggc gccgcttccc acatcaatga ttcttgcatc aggcggtgtg
     6841 atgtaccgca aggccgtaag agaatccagg aagtgtcggg caagaatatc gcgggccgat
     6901 ttttcagaaa tcagatttgt cttggcgttc cagtccatca attcgttttt atagacgtcg
     6961 aactgctgca gttgcccgtt cgaaagagcg atatttaatt gccgggcctg gtttttcaaa
     7021 aacgctatgt catgattcat tcctgtgcaa taacaaaaaa gatcaacagc cgcaaatttt
     7081 aaattatatt catggcctgt catcggcgtc catcgtgtca ttacaagggc tctccccgat
     7141 aagattcgct tgacgcacca gcgcggaggt catatactct tcaaacgttt cacgattaaa
     7201 aaaacaaatt aagtttaaga aggagacaaa aatgaaaaag ataattattt gcttttttct
     7261 gtgcggaact ctgatgctga ccggttgcgc acagacaggc acgatgacaa aaagtcagac
     7321 gggggggctg gtaggtgcta caggcggagc ggttgccgga gccgttttgg gtcaggtgat
     7381 tggccgtgat accaaggcaa cgctgatcgg tgctgcaatc ggtgctgcaa tcggcggcgc
     7441 cggcggctac ggtgtcggca caatgatgga caaccaggaa agggatatgc gcgatgctct
     7501 ggcgacgtcg gaagctgcag cggttaccag agaaggcaat ctcttgtcgg tcacgttcaa
     7561 gggcgatgtg accttcgata ccaactcggc ggaagttcgc cccggtcttt attcggaaat
     7621 caacagggtt gccgccattt taaatcaata ccctcagaca ttgattcaga tcgaagggca
     7681 taccgacagt gtcggatccg atgactataa catgaaattg tcccagcgac gcgccaatac
     7741 cgtcaaaacc ttgctggttc agcggggtgt tgccgccagt cgtattgaaa cgatcggcta
     7801 tggggaaacc atgcctgttg ccacaaacaa taccgaggtc ggcagacaaa tgaaccggag
     7861 agtggaaatc aaaattgctc ctcaaacgca ggcgcaaaca ccgtaaaaaa atttttcatc
     7921 gatacgaaaa ccgccgggac gcccgtgtca tgcgtgcccg gcggtttgtt ttgataacgg
     7981 aaaaattttc taagactgga tgatgtgaaa aagaataatc cataggggtt tcacaaaaac
     8041 ccctttaccg tttcaaacgc gcaatcaggt cattcataaa aacctcacgc tctttcagtt
     8101 cccggcgaaa ttccttgctg tctgtattgg caatttccat ttgaatatcc ggtaaatatc
     8161 gctcaagaat taataaaagt tcgttttctt cctcttgcgt caagttaagt ttgctcatga
     8221 tcgttcctcc tttcttttga taaaagcaat gttaccggta agttaactct ttacagatat
     8281 gcgaaattta caaaataccc gttttttatc ccgtttcata ctccattgat gtccgacagt
     8341 gttttgatgg atgaatatgc aggcaaacag gattgtgtca tcccgtgctg cctcctgcca
     8401 ggtttaaaaa aaacttaaag tcgttgacgt tggtttgctc acccgcgcgt ttttttgcat
     8461 cagcacgtca gcaaggcggt ggaatgttgt ctgaaaccga aagggatttt aacttaacgc
     8521 tttattattg tatatcaata ggtgcttttt cgtcaagatg caacatcatg atcctttacg
     8581 gccacgggct tgacgcgttg ccgcagacgg tatggtcatg atggaaaatc agccccgcca
     8641 caggaggaaa agaatcaata tcacaacggc aagatgtgcg gcgattacgc cgactttata
     8701 ccgctgataa aattcatttg ctttttgaat acgtttcagc ccgccgaata aacgacggat
     8761 cagggcgagt cggggatatt tgtaattgtc agaggtaaaa tttttttgaa aagcacacat
     8821 gggacagaca gcgtagacat cttccgtcaa cggccggaaa agcgtatcgt tgacaatgcc
     8881 gctccacggg tcgtaggcca gggtttttgc atagggagcg tttagagcgt aggcatgggt
     8941 cagactctgg tagcggactt tttgaacaca agggtcgtct gtgttgcggc tggaacggat
     9001 taatgctccc aacagcagaa ctttccaacc gggatgtcgt cgtaaaaaat cagtgcattt
     9061 ttgcaggcgt tccgggtcaa agcgatcaaa ttcgacatcg tcttcaaaaa cgacaatatt
     9121 ttttgctccc gcctcaaggc ccttgcgtag acacagcatg tgcgcctcgt atacttcgcg
     9181 ttcgaaatca tcggaaagac gttctccaat gacgaattca acgcgattgg ccaggccgac
     9241 tttttcaaat tccttccggg ccgactggcg gcgatcttcc cgtttgcgaa gcgagataca
     9301 atagaagcga tcgaaaaaat cccagccgtt ggttgaagag ttcattttat catcgtgtca
     9361 tgttaatttt ctttacgatt tttatcttat ccggcagagg gatgtcaagg aggggttcgt
     9421 ctcagaaaag ccgttgcaga aaaaaaatgt ctcatgtatg tctggttcac accgtgcggg
     9481 atcatcggat cgcctgtgac cggagatgat gtgttatgat caaaaagaac ctggggcaat
     9541 atcttgccca aaaaaaattc atccggaaaa tcctggatga ccccgtcgat ttaagcgagt
     9601 ttaagcaacg tccgacaccc cgtttgatcg gcggtttagt cctcatggcg ttgagttacg
     9661 taatcggctg gcccatcata tttgtcttaa gtattcttgc cgtttcgttt cacgaaccgc
     9721 tgatttttgt gatcggcggg ccgctggctt atgccgtctc ctgtgttgtc tttctgatcg
     9781 gcgcatggat ggcccgggcg cctcattaca tgaatacact gacccgttat gccctgcagt
     9841 ccctgctcaa aaaattattt gatcccgtca gaaccgattg aggactgcaa gccaggcttg
     9901 tgtgagcggg cgcgtttcag aagccccggc ggacagccaa gtggaaacgc tgcagaacgg
     9961 gtcaggcaag gcgggcggat attaacggag caccttaaga ttaggcggcg atccgacaat
    10021 gggcagctgg ataaatttta caatagacgg ttgaaatttt tcgaagtagt cgctgctttg
    10081 tcccggatcg gaggagggca gttccagcgt aagcgtaaaa atattacgtt catgtcctgc
    10141 gtaattgccg agcgacccag gataataacc gaattttttc aacgggtgat tactttcttt
    10201 gcagatctgc tccatccatt tttcaaagga atccaagtcc gatgagggac cgtcgtaatc
    10261 gaaaaaatta agaggggaat gcgcagacag tattttctgc ggtttgaatc gtttgattaa
    10321 agccatctga aaaatcgttt cctgttccga agccggtctg gttccggggt agtagcgaac
    10381 ctgttttccc ttggctatcc attggcggat ggctttggct ttccagtctc tggttgggaa
    10441 attccggtta atgtcaatgc cgctggcgtt ggtacgggtt ggttgagcgg ataagaatcc
    10501 gtcgggattc aatagcggtg caatcacaat acattgatcc ttaaacaggg cgggatgctc
    10561 ttttacataa tgggcgagtt taaacatcag gtaagccgtt ggtaattcgt ctccgtgcac
    10621 gccgccgaaa aacaaaacgc agtttttgga actattgccg aagtggacaa acatgagcgg
    10681 atgtttgttt cgggttgttc tgtgatattc ccaggaaatg tcccgcggat gaagatccgt
    10741 ccaccggtaa tcggcgaatt tacgttccag caaccgcaga tattgtttgc cctctctggt
    10801 ggaacaatca taggcaaaac tttgcagcgg cagcaataag aaaaaagcaa acagcaagac
    10861 cagaccaaca gagattannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
    10981 agcggcagca ataagaaaaa agcaaacagc aagaccagac caacagagat taatgatatt
    11041 ttcactttta ccatacgggt cagcgttcca atcttttcag gcaagcgtaa aacaaaaatt
    11101 taagctcata accacaccgt cctagggcgg atgatggagg tgaacgattc cagagagtcg
    11161 agtcgcatca tgggataaat gaaatcgttt gtcaatatct atacacccgt ccgggtgtcg
    11221 gccgggacag gcgaaaattc tccgtgcaaa tcgttgaggg ctgatttata cagacaaaac
    11281 ggaataacga ttttttagaa tactttcgaa acaggaaaca aaaccatgtt gaaaacgcaa
    11341 ttaaaaaata tcagtgttgt tttatacaaa ccgaaatatg cggggaatat cggcgcagta
    11401 gcccgtgccg caaaaaacat gggcattcgt aagatgattg tagtggggac cgatgatttt
    11461 gacagggaag aaatgcgaca gcggtccacg catctggccg ccgatgtttt agatgatatt
    11521 caatacattg aaaatgccgc cgaagcggtg agtccgttca attatattgt ggggacgacc
    11581 gcacgtacgg gcaaagcccg cggtccgttt gtgacgccgc gccaggctgc acagacgata
    11641 gcggatattt cgcagaagaa caaggtagcc ctgcttttcg gtccggaaga taccggcctg
    11701 gccaatgagc aattgcgtct ctgtcacatg gtggtcacga ttccgacatc gcgggaattt
    11761 acctctctca atctttccca cgcggtgatg attctctgtt atgaaatatt tactgcgtcc
    11821 tgtattgcgg tgacggcgtc tgaagcaacg ccgaagctgg cgctttcctc ggagctggaa
    11881 ggcatgtacg gtcagatcaa aacattgctg gccgatatcg gatttttgaa tccggaaaac
    11941 ccggactact ggatgatgca tttgcgccgc ttctttaccc gtgccggaat tttgtcgcgg
    12001 gaggtcaaga ttgtccgcgg tatctgccga caactgcagt ggtatgctca ccacaaaaaa
    12061 acttgacttt gcaggggagt tcccatagtg aatgcggcca taaacattgc ttttctaatc
    12121 gtttgattgc atgtatcaaa gatgcatatc tgatgaaaaa caacgtcaca atacaatgga
    12181 taccgatgga ggtttgacat tatgagaaaa acattttcat ttaccggcgc caaaaaaatt
    12241 gttttcggca gcggatcatt attaaccctg gcgagccatg tcaaggagca tcatgcccgg
    12301 aacccgctgg tggtgattga taagaacctg gccaaaacgg atttgctgga aaaagtcgcc
    12361 aacgttctga ttccgtccgg cattaaattt acagtctatg aaaaggtgga ggcggagccg
    12421 cccattgaac tggcggatga gggtgcaaaa gccgccatga aaaacaaatg cgatatcgtc
    12481 atcggcatcg gcggcggcag tgcgatggat gtcgccaaag ccattgccgt catcgcgaca
    12541 aacaagggcg cagccgccga ttatctcggt ctcaataaag ttcccaaacc gggacttccc
    12601 aaaatcatga ttccgacaac tgccggaacc ggatccgaag tgacctttac cgcggtgttt
    12661 cttcgcaaga atttgaaaaa gaaggaaggc atgaacagcc cctatctgta tccggagctg
    12721 gcacttttgg atccggaact gacgctcagt cttccgcctg ttccgacggc gcataccggc
    12781 ctggatgccc tgtgccacgc tattgaatcc tatacctcca ttaattcttc gccggtgagc
    12841 gagatgtttt ccctggaagc gatcgcgttg attgccgaaa atcttcgcac gtgtgtgcac
    12901 gacggcaaaa atatcgtcgc gcgcgaaaga atgcttttgg gcagtttata tgccggtatc
    12961 ggtttggcca atgcgggcgt tacggcggtg cattcgcttt cctatccgct gggaggaaaa
    13021 tacggtgtcg gccacggact ggccaatacc atgctgctgc cggcggtcat ggcgtttaac
    13081 atgccctcgg ctctggaaaa gtttacggat attgcggagg ccatggggga aaatgttgaa
    13141 ggattgcctg agcgcgaggc cgcctatctg gcgctggagg ctgtggaagc attaattgaa
    13201 gattgcggca tccacgacac catttcaaca ttcggtgtga aagaaaagga ttttccggct
    13261 ctggccgacg tggcagtaac ggtagccaga ccgctggaga ataatccccg taaactgacc
    13321 aaggacgata tgatcgcaat ttacacgcag gcattttaga aaggaaggtt aacgatatgg
    13381 caggtgcaga attaatagga aaagaagaaa tcaaagaagt tatggatgtt ctcgaaacgg
    13441 gcattctgat gcgttatggt tttgataaag aaagaaaagg catttataag gtcagagagt
    13501 ttgaacaggc gtttgccaaa tattgcggta ccgattacgc actgggcgta acctccggtt
    13561 cctcagcgct ccaggtagcg ctgacggcga tggatgtcgg tccaggcgac gatgttctcg
    13621 ttccggcttt cacattttta gcgacatatg aagcggtcat ggaagtgggc gccattccca
    13681 tcatggtgga tatcgacgat acccttaatt ttgatcccct ggaaatcgaa aagaagaaaa
    13741 ccccttacac caaggcggtc atccccgtgc acatgtgcgg ttcggcggca aacattgaca
    13801 agatcgtaga cgtcgcccgc aaaaacaagc tgctggttct ggaagacaac gcgcagggtt
    13861 gcggcgcgag ctataaaggc aaaaagctgg gcagcttcgg cgatatggga acgttcagct
    13921 ttgattacgt taaaacagtt accaccggcg aaggcggcat ggtgattacc aataacaagg
    13981 aattttatct gcggtcggaa tggtatcatg accacggcca tgaccataat cccaaagtca
    14041 gccgtgccct ggaaggacga accattctcg gtttcaactt ccggatgaat gagctgcagg
    14101 gcgctttggg cttggctcag ctgcgcaagc tggattatct catcggcgaa cagaagaaaa
    14161 ataagaaatt catcatggat gttctggcca atgtttcggg tgtgaaattc cgcgtcaaac
    14221 cggatcccga aggcgattcc gccacgttcc tggcgtttaa tcttcccagt gaagatcagg
    14281 cacagaaatt ccagaatctg ctggctgccg aaggcgtgga taccgtctgt tacaagagaa
    14341 atctatggca ctatgtgccg aactgggaac atttcctggc gaaatcgacg gccaactcga
    14401 aaaaatatcc gttaaccaat ccggcttata aaggaaaggt ggagtacagg aaggaaaata
    14461 ttccgcaggc cgaagatatt ctggggcgca cgctggttat gggtatttcc gtgaagatga
    14521 gccaggaaaa actcgacggg attcgaaaag gcatcgaaca ggccgcgaaa aacatgtaat
    14581 gtgccgatta cagggtggcg aaaagccacc ctgtgcctgt taattgttta gtttctccat
    14641 catgagatat tcttttcagc gactatcgcc tatccgtgtt tccagcaatc ccattgtgac
    14701 agtatctatc agagcatctc attattttcg ttatgagtcc gttctttagc ccgctgcctt
    14761 gataagccgg tcttgagcct tcaccttgcc caacagcatc ttgcccaaca gcaacgacat
    14821 ggagcaaatg gacagcgagg gcggagcccc cggcgatacg ggcatggtgc tgccgtcgca
    14881 gacataaagg ttttcaaact ccgtcgaaaa gtcacggttc acggcctgtc ccatggcaag
    14941 ggttcctccg ggatgtcctc ccgcccattt caggatggnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnngc ctgtcccatg gcaagggttc ctccgggatg tcctcccgcc
    15121 catttcagga tggaaatggt cttcggatcg ccgccggact taatgatgat ttcccgcata
    15181 atgtccgttc cttttttcaa tctcttttcg tcatttgcgg tgaagggctt gctggttttc
    15241 tcattgggga aaatttgtcc gtgggcttca tcggacaatt tgagaaataa ccccatgcct
    15301 cttttcatga tgggaaattt cggcagattt tctcgggctg tctgcatgcg gaaaaaagac
    15361 aaaaccgtcc atgttccaaa agcggaacag ttgccgatca taaatccttc gctctgagcc
    15421 atggattcaa tggcatgact gaacgtctgt attccccaca agccgccgtc gggatccttg
    15481 ctgtaaccca gcatgatgtt catgggatcc ataaagaatc ttgttcccac atcctgcgtc
    15541 cctaatcgtt tgacccccga tcttaacaat aaagcggggc tgccgatacc tccggcggaa
    15601 aggatgatat tgtcgccatg aatgacggtg ccgttcttta gttgaatgcc ggttgccttt
    15661 gtttttccgg cattgaagag gattttgttg accggagcat tgaagatgag ctcggcgccg
    15721 tatgatttag cctcttccac gtgttccctg gttgtccagc gcgcgttttc cggacaaccc
    15781 agcatgcacc agtcacaacc cagtttgcac ttatcggcat ggacgaactt ctcctgctcc
    15841 cggaagggaa tgcccatttt atcggctgtc gccagcatcc ggtagagtcc cttgttgttg
    15901 cggtaaaatt tttccggcag aacctcgata ccgatttctt ttttaagctg cttggcttcc
    15961 ggtctgaaat cgacccccat attattaatc agttttacgg gaggatccat gacgttggac
    16021 tgataaacca tggaagaacc gccgaccgta atgccgcgtt cgataatgac gccttccctg
    16081 gagcgggcga agagcatgta tcgctcaagt agcctgatgc cgaaaggaag ccccagaaac
    16141 tttttgtgat aatcgccttt ttccaccatg agcactttct taccggcttt ggccagttcc
    16201 ctggctgccg gagcgccccc aggtcccgta ccgacgatta tatattcata accgctcccc
    16261 ttttttttca tctgttttac cctcccggtt tattgtattt taggtttata atcagcccct
    16321 gccgcctgaa ccagattttc atcggttgcc tgtttgtttc tcaacttctc cagaaaagcc
    16381 tgaacccttg tttttatttg tccgatgcct ccgccgtaga gttccctctc caaggccaag
    16441 agcggcaagc ctcttttttt tgattctgtg cggtgaagat atatttcccc gccccaaata
    16501 tcgcaaaact ttaatttttc gataataatg ccgtcaatgc cgtattcatc cctgacgtcc
    16561 aacaaataat taaacctccg ttcaaaatca cccatcatcc ggggacagga taaatggctt
    16621 aagtatctgt cggcaatggc ctgcaaagga ttgccgtcat ttttgatctc atcaaggaaa
    16681 tgacgcgatc ccagacagat gttatcggcc acgatgacgg ccccgcagtt ttctatgagt
    16741 tccagatgat ccatttcctc aatgcagcct ccggagaaaa acagccgtaa atcctggggt
    16801 ttggacacaa cgcgaccggt gatataattt ttcacattcg acaggatgtc tatggcccgt
    16861 tctacgggaa ccgctgtgac ggccagcata acgcccaata actcactggc ttttatgggc
    16921 acatccttca tttttctcag ggcatatatc tgggccagaa gacgccgctt ctgattataa
    16981 aggttgatgg aatgcctgag cgattcgttg tttatggaaa tatgaaaatg tttctcgatg
    17041 gatgatttta gcttgctgca ctccagggaa aaatagtcaa gagctgcttc attgatcata
    17101 tgcggtgcga aaaacatgtg aagaaaagga gggtcgatct ttgcgaaacg ccagttgtca
    17161 tacatgcgcc gcgaatgatc acagccgttt acgaacacaa cgccgtccaa aaacgcaaaa
    17221 tctcctttga gcgccttttc gaaacagtgc ttgacgaagg tgcagttgat cgaagaataa
    17281 aaagcatccg cacgggttgt ccctttgctt tccacggccc gcatgcggta gggaacaaag
    17341 ccggccgcgt ggataatttc ctcaggcata tatgaacaga aatatccgat gatttttttg
    17401 ccctgttttc tggcttcttc aagataaggg ttatgcgtcg atgatgccag ttctcttgtt
    17461 tcgtcgagta tggttttcat acaaatccgt tgctcctgaa aaattccgtt atccagcgtt
    17521 tgatgtcatc ctccgttgca tgtctctgat ccatgatatc catattcatg aacagcgacg
    17581 gcaatcccct ttttttcagc aggtctttga taataccaat gaccgcccag ccgtgtttac
    17641 agccggcgtg tcccgcgaag atcaggcaat cgggcgagta ctcgtcgatg gccttctcaa
    17701 gctcttgcgt aataaattcc accggtccgt gacattgtct ggccatcgcc aggtaaagat
    17761 gatttttagc catgtcacga atcattgttt tttccgagga cgtatcgatc tgcggcatct
    17821 ggacatcgcc gataaaatcc ttgacgacga cggcgccgaa ttcatcttcc atccacttga
    17881 agacgtaggc aaaaaaagca atcggcgggt tccaccacaa aacgcggata ttttcctttt
    17941 taacgacacc gacacctttt ttaaagcgat cctttacgac ctcgtacatg cgtctggcca
    18001 tttcggtgcc gttaggatcg ccgatggcga gttcccgcac gacccaggca aaaaccggcg
    18061 tgatcatcgt tgcgggagag gggattgccc gcatcatttc gcagatctcc tgcatataaa
    18121 agttgaattt gttttcatga tctacaatct gtttgagccg ctcccagttg attttctggt
    18181 gaagggtttt ttccaggaaa tcgatgaggc gccacatatt tttttcataa taatcgaagg
    18241 cgtctttatc gcgccaataa ggggagtcga aaaagaacac cggggcaccc gtcagatggc
    18301 gtattccctg ataggcggca acgttggtgt cgcagggatg cgacccggca atgattgccg
    18361 cgggtttggg ggtttgcccc aacatgtagg ctcccgccgt cagcttgatg gccgagcagt
    18421 attctgaggc aaaaccagcg ctttccgctt cctctatgag cagcgatgga acgtcctgtc
    18481 cggacaagtt tccgaacaca ttaagcgttt ctggattgaa cggaaaagcg tcaaaagcat
    18541 ttacgatttc ggaggaaaga atccattcca cccagacgac gggcttaccg ttttcgttag
    18601 cttttagagc atttttcata ttatcaacaa aggcttttgc cccatagtgg taggctttga
    18661 tttcctcggg cggaagaatt cctcctccca gaatcaggaa agcatatctg aatttgtgat
    18721 tgaagaatgt ttcgcgtttg acatagagag gccgggtaag tctgagagcc cagtaactca
    18781 aattcggaaa agttgtaagg taatatccga aattcagaaa actggagctc catttaagat
    18841 attgaaacgg cttggggtta tttttcaatg aaactctcct tctgattcta ttaatatttt
    18901 ttcggcaaag ttaactccgg ctgctgtgat aacttgcgga agaagaaccc ggcgttgaat
    18961 ccgcgtttga tgcggatggc cgcctccgga caggccgctg tgcagtcgcc gcaggccata
    19021 caaagcgata tccccggtgc ggtttcgatc accagcggta tcttcttttt ggcactgtcc
    19081 tgccttttgg taagcatcag gccccgggcg ggacagatgg aaacgcagat gccgcagccg
    19141 gtacatagcg tagggtcgaa gacgacaacc ccgtaggtca tctgagcctc tttaaagtaa
    19201 ttgggaatct ttcccttctt tcctaatgac agatctaaca tatcacaccc cctgcctttc
    19261 gattcgtttc tccccgccgg cctcaagcca ttcgacgagc tgcacttccc tggtcacctc
    19321 gccgtcatag tcgcctttcg gccagcccaa aacaatacag tcggtcagct cataggggga
    19381 tttaactccc aattttctcc gccacagggg attcatcatc aaaaccgtca tcatgccgat
    19441 ccagcatgtt cccaacccca ggctgtgagc ggccagaacg atgttttgac cgcagatgcc
    19501 ggcatcgagc accggattgc cgatcccccg cttatcgatc aaaaggagaa tcataacggg
    19561 ggcgttatag tacacggctg tcttttcggc ggcgatctgc tggagcaggt tgaaggggat
    19621 gggatggagt ttgttgggca tcagccgggt cataaacttc gtatagggcg ccagaaatat
    19681 gcgccggaag gcgtttctgt tatagtccac gaaccacatg agtaatttac taagctgtat
    19741 cgttgcccgc tccatttctg aaatcagcgc cgggtccttg acgacgataa acttccaggg
    19801 ttgggcgttt cccgccgtgg gggcgaatct tcccgcctcc agcacccgtc ggatcaggtt
    19861 gtcgggcacc ggttccttct tgaagttcct gatgcttcgc cgttccatga tcaccttttc
    19921 cacgggattc cattccatgc cctcctggta ggtgtagagc ggggaaagat tgtcggttgt
    19981 ttgttttgcc atggtatacc tcttctctcc ttgtggggtt aaagggtcaa taaatgccgt
    20041 ccttggtctt ccagaggaag gctacaaaaa agatcgagaa tacgaaatcg cccgataaga
    20101 cgaagacggg aaaccaggcc gcttgctcca gggcgtagta gtagacgaag gtgaaaaaga
    20161 tgatgagctt ggcgacaagc cccagccaga tcacggcccg gttggccagg agatcgcgcg
    20221 agacccagta gtaaccaatg ccgaagagga tgacagcgat cataaagaac ctgaaaaata
    20281 tcaaggtctg gaggttgtcc gttaccggca ccgtaccatt gataaaggcc aggagattgt
    20341 cttctatcgg cccctttccg aacatcatca tgagcataaa atccgtaaac aacagactgg
    20401 ttatgccgat acccagattc cagagcgaac cgacaaaaaa catcttctgc cagaaacccg
    20461 tattgatctt gctcatagtt tttgacctcc tttgttacta gataatggta atttttataa
    20521 tcagacgcat cgagaaaccg tcacacatag gcttttttgt tttcgtccca ggtgccggtg
    20581 acgaccttca gcgcctctaa ttcctttttc ttgacccggt gcgtttcagt tttcggcaat
    20641 tccggaacga tcttcacata tcggggcacg gcgaacttgg gcaggttttc cttcagatac
    20701 tcgatcaact cggtttcttt cagcgtcacg ccttccacca gagtgatgga ggccatgata
    20761 tcatcttccg ccagttctga cggaacggcg taaacagccg cctccagaac attggaatgt
    20821 ttctggatcg cctgctccac ctcgaaagcc gatacatttt cacctttaat gcgcatcgac
    20881 tctgtgtttc tgccgataaa gtacaggaag ccctctgtat cgcgtctgat caaatccccg
    20941 gtgaagatat aatccccgtt cattttttcc gccgacgctt tttcgttttt atagtactcg
    21001 acggaggatg tgcttttctt attggaaacg ggaaagacca gctcgcccgg cgtaccatcg
    21061 ggcacatctt tcatgttggc atccacaaga cggtacttga ctttcatggg cggcttgccg
    21121 atcgatccca ccggcgcagt gcccaggttg aggatgatct tgccgcctcc gtcaacggcg
    21181 ccataggttt catagatggt cagtccgaac cgttgttcga atttttccca caaatcggcg
    21241 ggacaggccg ccgaaataat aaaacgaacg ttgtgctggc ggtctgaagg tttttccggc
    21301 tgcttcatca gaatagggat catggctccg atggtattaa aggtcgtaac gttgtacttt
    21361 cggatatcat cccagaaacg gctggccgag aatttccttg ccagaacaac ccgggctccc
    21421 gtatgcaggg cgcaggtcac tgttacaaaa agggcattgc cgtggaaaag aggcatgccc
    21481 gtatagagaa cgtcgctttt tttgaaaagc ataacggcga ccagcgacaa cagtttcacc
    21541 gatgatttac ggtagcggta aacaacgccc ttgggaagcc cggtcgtccc ggaggtatag
    21601 gtaataaaac agatatcgtc tttgcggtag ccaagccccg gattcgcggg cgatccttta
    21661 taggcttcat caaggacatg atatcccgcc ggatattgtc tcgatgaaga aggggatgga
    21721 ttaatgatga tggtttttat cttatccaac cggtcggcta ttttgttcaa ggtctccaga
    21781 tgcgtttcat cgatgaccag atattcggcg tcacaatggt taaggatata caggagactg
    21841 tctcccttca acgatgtgtt tacggggacg gcgtacatgc cgatcttctg aatcccgatg
    21901 aaaatatcga tgaagctcgg tgaattgtcc ataagaatcg ccacgccctt gccctgccct
    21961 gcgcctgaag ataaaaagta attggctatc cggttggcat ttgcgttcat ctgacgatag
    22021 gtgaacgttt tgtcttcaaa gataagaaaa attttatcct tgtaacgggc cgccttttct
    22081 tccagtaatt ctccccacga catttcttgt cgaatttccg tcctggaaac gccgcgaatg
    22141 ttcttcagaa aggaacctct cctgatttca ttgatggaaa acaaacagat cgatttcaaa
    22201 atgttttttc ccagatccag ctttcttagt tttctgttca ctgctttgct ccttacatac
    22261 agattatttc aatgacgggg cttccagaat atggatgcac atcgcggcct cttccactcc
    22321 cagggctccg ccgccatttt cagcgagtgc aagttttgcc ccggatacct gtcttggccc
    22381 ggccgcgccc ctgagctggg tcaccagttc atggatctgt gcaaggccgg atgccccgat
    22441 gggatggccg cgcgattcca gaccgccgct gggattcacg ggttgcttgc cgcccaattt
    22501 cgtcgccccg ctttcggcat atcgtcctcc ttcgcccaga cggcagaacc ccagcgactc
    22561 gcattgatga agttctccgt aggcggtggc atcgtgcact tcggcaaaag ctatctcggc
    22621 aggtcccacg ccggcctttt cataggcgag gcgactcagc cgttcgccga tgtcgatacc
    22681 gtcgcgatct ctggccatgc cggaacccag aatcgacgcc ctgactttta ccgcctggcc
    22741 cgacaatccc aggcgtctta ccgttgcttc gtcgcagaga atcgccgaag cggcgccgtc
    22801 gccgatgggg gcgcacatgg aacgggtcag cggccaggtg accaggcggt ccgccaaaac
    22861 ctgctccgtc gatactaaaa attggtattg tgcattgggg ttcagggaag aatgaaaatg
    22921 atttttggaa gcgatggcgg ccagatgttc gatggtggtg ccgaactttt tcatgtgctg
    22981 acgcgcttca tagccgtaaa catccataaa gggagaatgg tcgccgctgc ctaatttacg
    23041 cagtttcttg agcatgtcgt aacccatggt ctcgccgatc cgaatggcga caaccagggc
    23101 atcccgctgg gccatgaact tgtccttgaa ggattttttt ctgcccttgc cggagctttg
    23161 ctttgccagg cccttgtact tttcgatatg ggtcgccatc gccttctttt cgttttcgct
    23221 taaagcgacg tcgcccaatc tttccagcgt ctttccaaag ttgccgacat ccaggccggt
    23281 cagaaaaccg gaaaaaacga gcgcctggtt ctgatcgtaa attttttccg cacccagggc
    23341 catcgtgatg tcatacatcc cgctggccac gcccatccag gcatgatgaa acgccgtgga
    23401 accgccggca caggcgtttt ccaggttggt gatcggaatg gcatcgatcc ccaaaggacg
    23461 aagagccacc tgaccgcgga tgcagacctg ccttttgtat tcaccccagg cggaattgga
    23521 aaaccagagc gcctggatat ccttcttgtc caatccggct tctttcagac agggggaaac
    23581 cgtcattgcc gataattcgg atatcgtctt ctccaggtat ttgccgaact tgattgtgtg
    23641 ggccgcaacg acataaacat ctcgcataat ctactccaaa aaatatattt aaaacaggat
    23701 tgagggatag gtcctctttc tttgtcttat tttttaacgt ttaatatttt cccaaaggct
    23761 ccaatttgct ccagaacgga tctcatcccg gcggcagcgg gggatgtgcc ggcaattttc
    23821 gcgatagaaa ggccctgcct gtccagtgcg ctgatggcgt tgtccggtct caagctgccg
    23881 taatagtcca ttcccgcctc cagaatcatt ttcaccagat tttcatccgc ctgatcgaca
    23941 cggttaaaaa cagttcccac ctttaccggc gacagctggg gaattttttt cagtaaactg
    24001 gaaatcatca ccgctgtcct ggctcctctg agagagttat ccgtaacaat gacgggataa
    24061 tgaaccgatt ccacaacctg gcgattcact tgttcgatgc ctgcttccgc gtcgatgatc
    24121 accgcttcgt actgtgatgc tatcgacttg atcgtactgc gcagaagatt gttaacgggg
    24181 caataacatc ccagggtgtt ggtctgtccc atcgccacca tcccgaaacc ctgaccttcg
    24241 taaagcgctt ccatgagcag atagtcgacg atatcggcca gcttctcttt ttcttcagaa
    24301 gaactggaaa ttcttgcctg acggatgatt tcctcccgcg cggcgccgat ggtcttgatg
    24361 tccgaaacct ccagggcgct ggtcaatccc atggccggat cggcatccac aaaaaggacc
    24421 tttttgccgc aagcggaaag gatcgttccc gtaagagcgg acaacaccgt cttgccaacg
    24481 cctcccttcc cgaggaaagc gaggataatc ggttcgttca tgatcacgtt ctccttttta
    24541 caataacatc ccgcaaggta tcgcaggtgg gtttctccgt gcaattttcg cagtcaagat
    24601 cctcgcaact gatggttccg tccgcttcca gaaccagctt tttattttcg ccataaatga
    24661 tgcgggcgcg gttatgaatc ggaataagat gccccacgaa gtcggcatta tcggcgataa
    24721 gggcgacttc gatgtttttc agcccgggga tagactcatg ataggcctgt ataagacact
    24781 gtccgaggga ctggagggaa aatttcctgg ccaggagatc ccgatggatc ctgatccata
    24841 tcttgccggg aatgctccgg gtcatgtatc ccggaatccg attcgtcaga tttgagaggg
    24901 tttgtatatt aggacccgtg ggatcaaagt cacnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnngcggcgc caaaagcggc aaccaactgg ctgtctattg gcaacgtcgt
    25081 catgcttctg ccggtaagtc ccgttaacat gtctctaact gccgaaacct tggaaagacc
    25141 gccgatcaaa acgattttgt cctttgcgcc ggcgccatcg atcagggaag cggtctttga
    25201 ggctatggcc cggataacgc cggccagaat atcgcttggt ttcattccgg tattgacctg
    25261 cgaaatgatc tcgctctcgg cgaagacggc gcaactgctg gaggacgagt agggttggcg
    25321 ggaagcggcg gcgcaggaag aaatttcagt aaaaggtacc tctgccgcct cagcgaccat
    25381 ttccagaaac ttgccgcttc cggcggcgca tctctcgttc acatgagtgg tctccagaaa
    25441 accattttca tcaatggtag aaaccgtgat aaaaagcccc cccagatcaa taacggttct
    25501 aatatccctg tcaaaaaaga aggctgatcg ggcagtgcag gcagcttcgc ccaatgtaaa
    25561 ggcggccttt ttcaccaaat ggccgccgaa accggtggcg acaatttttt ttatttccca
    25621 tcttcccgcc cgggcttgaa cccgcgcttc ctttaacaaa gaacggataa gggcatcgaa
    25681 ccggggacct atctccgcgc aggctgaccc gcgcagatgc tcgccatcgg ttacacaaac
    25741 tttgataaat cgggttccca cgtccactcc tgcagtaatc acattcccac cttcgctttc
    25801 aacgtctcca tggcatagac ggcggcgccc attgcgccga ttatttgggg atccacagga
    25861 agcgatgtaa ataaaacgtt catatgataa gccaactgtc tcaccacgcc gggattcttg
    25921 gatactccgc cggtgataca aatgtttttc tcgacgtcaa tggctttggc cagagctgca
    25981 acccgtttgg caacggcatc ggcaatgccg caggcgatgt gtttaagttt ttttcttgcg
    26041 tagagatgat tgaggacttc cagttccatg aagatactgc atttgttggt aatactgatg
    26101 gggttcttcg atttaacggc caggcttcct aacgtctcaa ggtcgacgcc gatcgttttg
    26161 gccagcattt ccaatgatct tcccgtgccc gcggcgcatt tgtcgttcat gatgaaatca
    26221 cggacattgc cttccgcatc gatcaggata atcttgcagt cctgtcctcc gatatcaata
    26281 atggtcctga cgctgcggtc cgcacggaag gcacccaatc cgtgacagga tatctcgctc
    26341 atattcatct gggcgaaagg aatttcgtaa cggccgtagc ctgtgctgca gcagtagtcc
    26401 agatcggaaa atcggagctt tgccgatgcc aaggcttttt ccattacttc cgtggccgaa
    26461 tccagggtag acaatctcac ccgggtgatc tctcccgcaa gccggccctt ctcattcatg
    26521 atcaatgcct tggcggtaag agatccgaca tcacatcctg ctacaatcat tgttccctta
    26581 aaaactca
//
LOCUS       DAONQZ010000028        26308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2023_length_26308_cov_16.531482, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000028 DAONQZ010000000
VERSION     DAONQZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 26308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26308
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_26308_cov_16.531482"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..708
                     /locus_tag="PLR13_06310"
     CDS             313..708
                     /locus_tag="PLR13_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQC18985.1"
                     /translation="MKEISLKTLSRVEMIDITASVQEAVRDEGVQSGVCLVYTPHTTA
                     AITINENADPDVQRDMIAALDRAVPLSANYRHAEGNSAAHVKSSLVGASELVIIENGH
                     LVLGTWQSVFFCEFDGPRTRKVFISMIAG"
     gene            1183..1368
                     /locus_tag="PLR13_06315"
     CDS             1183..1368
                     /locus_tag="PLR13_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC18986.1"
                     /translation="MGKGLKGFRDYMDYLTYAEIYLFWIDGNEHKKTYNMLMKRQGDI
                     EPIRYMRKMSLAAHSQN"
     gene            1973..2266
                     /locus_tag="PLR13_06320"
     CDS             1973..2266
                     /locus_tag="PLR13_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC18987.1"
                     /translation="MTKIREAAEKGIAYSQYVLGEMYYKGKGIPQDSDEAVKWFRKAA
                     KQGEVYAQYNLGWIYGTGTGVPQDYAEARKWYRRAAEQGNASAQLRLNEMKDK"
     gene            2394..3824
                     /gene="pyk"
                     /locus_tag="PLR13_06325"
     CDS             2394..3824
                     /gene="pyk"
                     /locus_tag="PLR13_06325"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQC18988.1"
                     /translation="MIKKRKTKLVITMGPALQDRYLLREALKLADAVRLNASHSRPEE
                     RTPILKLIRNISDDLGRIIPVFLDLQGPKWRIGLLEAPVNLEKDSIGVLYLAGTPAPE
                     GYPWVAPLPHQELFTGAKVGQIWVLDDGALKLDVMETHHQQIMLKVLVGGMLKARKGV
                     HPIGLDVAFDPLTPQDLEDVRWGVQEGVDLFAQSFVRRASDVEALNLHIKELGGPQTI
                     IAKIEHPQALDNLEEILHVSWGVMVARGDLGVEFGVEKVPALQKQIIRKARQALKPVI
                     TATQMLESMIENPQPTRAEASDVANAIWDGTDAVMLSAESAVGKHPLEAVRYLHSIAA
                     DADAHYKPRIGLLADTLEENLSSRTDVSVAFAACRTAEEIGAKLIVVFTEGGGSARLV
                     SRLAADIPVIGATTDIGNSRRMGLLRGVESLLIPRAKHLSEMLASIQPLLKIRKGLES
                     GDRVVMTLGHPLWTTGTTNMMRVETY"
     gene            complement(4044..4829)
                     /locus_tag="PLR13_06330"
     CDS             complement(4044..4829)
                     /locus_tag="PLR13_06330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQC18989.1"
                     /translation="MRELKSFAVEMARNAGALLKKKFNKTHKIQYKGDINIVTEADKM
                     SEHLIIKSIRRNFPEHGILSEESPAIAGAGKKRWIVDPLDGTTNYAHGYPVFCVSIAL
                     ESDGNVVLGVIYDPMRDDLFSAVRGKGAYLNNKKLSVSSIRDISRSLLATGFPYDIRE
                     SRDNNLDYFNRMAVKVQAIRRAGAAALDLAYLAAGRFDGFWELKLKPWDTAAGCLMVE
                     EAGGRISDLSGKKWDIASPGVLASNGLVHKKMLDVLRMKPLGG"
     gene            4979..5776
                     /locus_tag="PLR13_06335"
     CDS             4979..5776
                     /locus_tag="PLR13_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQC18990.1"
                     /translation="MFIEQTPSDNSQKLKMKRESLGLSPDDVFKKIRIRTRYIEAIEN
                     SEFDQLPDPVYTKNFIKTYARFLNIDENPVLQDYDAYLNAHREEQTPPPEPPPETKSF
                     FASFAARKNYWGILLVLIIFISIWLILKQNQSVPEDINTPAAATRAPEATLPAAPALT
                     DSQTPATVPAPQTGVTDNPPATTDAKKSVLLITAGQETWLRIKPDNSPPVQLLLKPGE
                     KFESSEEFFNLDIGNAGGIKIQYKGRSIENLGKPGEVIHIRLPQVKQ"
     gene            5794..5979
                     /gene="tatA"
                     /locus_tag="PLR13_06340"
     CDS             5794..5979
                     /gene="tatA"
                     /locus_tag="PLR13_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQC18991.1"
                     /translation="MFGMGWQELVIVLIIVVIIFGVGKLPEIGSALGRGIKNFKKSIN
                     EQNEITDNSSKKEEDKK"
     gene            complement(6170..6925)
                     /gene="tpiA"
                     /locus_tag="PLR13_06345"
     CDS             complement(6170..6925)
                     /gene="tpiA"
                     /locus_tag="PLR13_06345"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQC18992.1"
                     /translation="MRKWIVAGNWKMHNTIAESIMLAKAIQDGSSDIQGGAVILAPPF
                     TALSAVGEAIKGSCLELAAQNMHYEDKGAFTGEVSPLMLKDIGCRYVIIGHSERRKYF
                     HEQNADVNLKVNKALSDGLIPIMCVGETDEEREKGITRDVVGRQVNHGLKGVEKIDPI
                     VIAYEPVWAIGTGKVATPAQAQDVHSFIRGILKESYGDVAGKISILYGGSVTRDNIGD
                     LIAMEDIDGALVGGASLKAEGFLGIIKTVAGGR"
     gene            complement(6965..8143)
                     /locus_tag="PLR13_06350"
     CDS             complement(6965..8143)
                     /locus_tag="PLR13_06350"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQC18993.1"
                     /translation="MKYIDQIDMKGKRVFLRVDFNVPMDKEGNVTSDKRVRASIPTIT
                     YALEKGAKLIVASHLGRPKGKRVAEMSLKPVVKVLSDLLKKEVLFLEDCVGPVVEQGV
                     ARLQDGQIILLENLRFQPGEDKNDAQFAKQLAALCDVYIDDAFAVSHRAAASNSAITE
                     FVPVCAAGFLLKNEVEYFKKAMVNPAKPLAAIIGGAKVSDKIGLLENLIEKVDKIIIG
                     GGMAFTFLRAAGYETGKSLCEEDMLDTARKIVDKAKQKNVQFLLPVDAVIAQAAEAQA
                     EARICGVQDIPKEWIGLDIGPATIALFSEALKGVKTVIWNGPMGMFELAPFSKGTFQL
                     AQAVADSGALSILGGGDTDTAIKKAGLTSKMSYISTGGGAFLEWLEGKTLPGIAALEK
                     "
     gene            complement(8158..9156)
                     /gene="gap"
                     /locus_tag="PLR13_06355"
     CDS             complement(8158..9156)
                     /gene="gap"
                     /locus_tag="PLR13_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQC18994.1"
                     /translation="MSVKIAINGFGRIGRLVFRAGYQNKDVDFVAVNDLADAKTLAHL
                     LKYDSTHGILDADISVANNAIIVDGKELKTFSVREPETLPWKDLGVDVVLESTGKFTD
                     KTGAGKHMEAGAKKVVVSAPAKNPDVTFVLGVNQEVYDKNKHHIISMGSCTTNCLAPI
                     VKILHQEFGIEYGLMTTIHSFTNDQVVVDEPHRDLRRARAAALSMIPTTTGAARAIAE
                     VIPEMKGKLDGLAIRVPTPNVSLVDFVATLSKSTTRNEINDKFREYAQSAMKGILACS
                     EEELVSKDFNGNKHSSIVDMPNTTVIGGNMVKILSWYDNEWGFSNRMLELLSFIMK"
     gene            complement(9311..10840)
                     /locus_tag="PLR13_06360"
     CDS             complement(9311..10840)
                     /locus_tag="PLR13_06360"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HQC18995.1"
                     /translation="MIQQPGFDNEKYIQEQTTEILKRVNRFNNKLYLEFGGKLLYDYH
                     AARVLPGYDPNVKMRLIRELKDKADIVLCIYAGDIERKKIRADFGITYDADALKLIDN
                     LRTWGINVLGVVITRFEEQPAAIIFKNKLERRGISVYTHRFTKGYPTDVETIVSDEGY
                     GANTYIETDKPLVIVTGPGPGSGKMATCLSQLYHDYRRGIKSGYAKFETFPIWNIPLK
                     HPVNVAYEAATADIRDFNLIDPFHLDAYSEKSVNYNRDVEVFPVLKRILEKITGEPSF
                     YKSPTDMGVNRAGFAIIDDAITQKAAKMEIIRRYFRYRCEYAMGFADRETVQRVELFM
                     KDFNLEPEERPVVQPAREAAVTAPVETRDYERGNEGIFCGAALELKNGKIITGSNSSL
                     MHAATSMVLHAIKHLAEIPDKIKLLPPQITDSVKNLKTEILDEKSVSLDLEEALIAIS
                     ISATNNPAAQLALEKLRELRDCEAHMTHIPTPGDEAGLRRLGINLTTDPNFSTNDLFI
                     T"
     gene            complement(11206..11742)
                     /locus_tag="PLR13_06365"
     CDS             complement(11206..11742)
                     /locus_tag="PLR13_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQC18996.1"
                     /translation="MKKKDKRFVGSLAFVFALLFVISSAGAALADAVSAKIGFVDIAK
                     IMRDSRAAQNARAVYQKDFESKKATLKAKGDRVSSLEKDLSNTKQDSPALKEKREKLA
                     QEFKEFKRLESDLNEQLKKKDAELTRKILMDVQEILKQFSKNEKYSMIFEKKAVLVVD
                     DGFDVTDKIIKIYDAQKK"
     gene            complement(11779..13434)
                     /locus_tag="PLR13_06370"
     CDS             complement(11779..13434)
                     /locus_tag="PLR13_06370"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HQC18997.1"
                     /translation="MANRSTQKRAVKIPRGKALLCDPGLNKGTMFSDTERDVLGLRGL
                     LPPRVFSPSDRTVHIINNVRRKTTDLEKYLYLSGLQNRNERLFYRVLIEHIQELMPVI
                     YTPTVGQACQEYSSIFSEPRGLFISKNDRGSIKKILKRWPQKKVRVIVVTDGERILGL
                     GDLGANGMGIPVGKLALYAACAGIDPEACLPVTLDVGTNNPELLAAPDYIGLPQPRVR
                     GREYDQLVDEFMAAAANVFPGVMIQMEDFGNANAFRLLEKYRSKYCMFNDDIQGTASV
                     ALAGLYAALRITGGSISRQRLLFLGAGEAAIGIGDLVSSAMMEEGLSLPEAHSNCWFV
                     DSKGLVVKGRTDLVEHKKPYAHDYPFHRDLLSAVEALKPTAVIGVSGQPGTFTPEIIK
                     AMGRINRRPIIFALSNPTSKAECTAEAAYRHTEGRAIFASGSPFGEVSMEGKTFVPGQ
                     ANNAYIFPGVGLGVLTSEAVRVTDEMFTAAAKSLNEQVSADDLKIGRIYPSLTTIREV
                     SAKIALAVAEVVFKRSLTRMQAPADLRGNIRATMYEPDFGTYI"
     gene            13679..14005
                     /locus_tag="PLR13_06375"
     CDS             13679..14005
                     /locus_tag="PLR13_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HQC18998.1"
                     /translation="MAEKKGLSKPVKLKGDLAELLGAKALPRTEITKKLWDYIKANKL
                     QTTKVNGKPENAGKNIVIDAKLIKIINNTKVKTSSGKVVDFTKLKEGQTIDMMQIASV
                     VSANVE"
     gene            14227..14793
                     /locus_tag="PLR13_06380"
     CDS             14227..14793
                     /locus_tag="PLR13_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQC18999.1"
                     /translation="MDKKAILWPVIISVVIHMTLLAVAGMIDLNDKIKPVNVLSVSIQ
                     EPAPAQPSPEKENPAQGNPSSSVTKQQNDPTPDDAWREDTINLGSLDTKYIHYLTRVK
                     NKILRIWKYPQTSFERNEEGNVVVRMSIDADGRLAAVTLVSSSGSADLDNGALGVVQE
                     AAPYEPLPGSYNLSRLHIVASFNYKIMD"
     gene            complement(14834..16453)
                     /locus_tag="PLR13_06385"
     CDS             complement(14834..16453)
                     /locus_tag="PLR13_06385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19000.1"
                     /translation="MKVKFLGAAREVTGSCFIIETDQARFAVDCGMHQGGADVETRNW
                     DTEAYDPSHIDFFLITHAHIDHSGLLPRMVQNGFKGPVYATEPTGELIKILLLDSAHI
                     QETDAKSKTRRMQRNGRTEKSEPLYSTPDAEAVSPLIKKITYNQNFTPAAGIKINFQD
                     AGHILGAAILELFIEENGTSTKLVFSGDIGRRHQLLMEDPVTISDADYLFMESTYGDR
                     DHKGEEDSLNEMAEAIQYSYSRGEKIIIPAFAVERTQEMLYSLYLLDIKGKLPKDMPV
                     FLDSPLAIKATEIFRRHHTYLDADTQNILKNGEDPLDLPQLKFSSSTEQSMRINEMEG
                     SAIVISASGMCNAGRIRHHLRHNLWRPGASIVFVGFQAQGTPGRKIVEGAKKIRIFNE
                     DIAVKAKIWTIGGFSAHAGQRQLLDWLGHFQNKKMPVYLVHGEAGAQDTLASLIRERL
                     GFEVAIPEYLEEINIKAGTRPQEIKQPRPSRPPLELAPLLADLKAKIDYINARVDKIQ
                     SLPESEQQEVIQLLRQVNQNMDDIKPDLQTG"
     gene            complement(16607..16822)
                     /locus_tag="PLR13_06390"
     CDS             complement(16607..16822)
                     /locus_tag="PLR13_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19001.1"
                     /translation="MFDETGPFYYSCKGLYSATDDATMKFYLLTGVQTMSKSMDKGKK
                     DAKKKPAKSAKEKKEAKKLKKAAKAGM"
     gene            16901..17830
                     /locus_tag="PLR13_06395"
     CDS             16901..17830
                     /locus_tag="PLR13_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC19002.1"
                     /translation="MIETRPIEPVIVARNLEKVFGAFKAVDDISFHVVPGVCFGILGP
                     NGAGKTSTIRMLYGFSPLTAGDIFVFGLNIKTHDRKIKARIGVCQQENTLDPDLTVMQ
                     NFEVFARYFDIGKKTALARATTLLKFFALEQRQNARATELSGGMVRRLVIARALINQP
                     DLIILDEPTTGLDPQSRHQVWERLEQLKNQGMTILLTTHYMDEAARLCDNLVIMDHGK
                     ILAQGSPRSLIREYTGGHIIEVAEPDEALRAFSQEKHDRVEDLGHRLIIYAANGHELY
                     QELCDRFSQKSCILRSATLEDVFLKLTGRELRE"
     gene            17827..18606
                     /locus_tag="PLR13_06400"
     CDS             17827..18606
                     /locus_tag="PLR13_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC19003.1"
                     /translation="MTFRISTRFIRVWQRNLTVYRESWKINFMPPLLEPLFYLLAFGI
                     GFSGLIGSVTYAGTEISYVMFIAPALLAINIMNNSFFENTFGSFVRMYYQKTFDAMMA
                     TPLTVDEIVTGEIVWGATKSVIGTIIMLIVIGFFGLISYPAGLLIVLVAFIGGFAFGS
                     VAMIFTGLVGKIELFNLPVFLFITPMFLFSGTFFPISGLPLWAQYFALTLPLTHLVNL
                     VRALGLGAFNAEALAGFCYLIIFCLITFPLALSVMRKKLIK"
     gene            18879..19214
                     /locus_tag="PLR13_06405"
     CDS             18879..19214
                     /locus_tag="PLR13_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQC19004.1"
                     /translation="MNKNLYVGNLSYKITGDDLKSNFSEAGEVVSATIIKDKFSGQSK
                     GFGFVEMKTEEGAAEAIKKFHGGTLDGKEITVNEARPKKEFSGGHRGGGNRGGGGGFN
                     RGGRRDGRY"
     gene            complement(19371..19982)
                     /locus_tag="PLR13_06410"
     CDS             complement(19371..19982)
                     /locus_tag="PLR13_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC19005.1"
                     /translation="MKKKNTFAELKNNEKRARQGVIVEAAQRVFAVKPFNKVSIRDIA
                     REAGISHASIYRYFPDQQSLFVEAFLHGAEEITAILDRMIKNSRKNPIEKVADAYLSY
                     LIDNDQYFRMMTRFMLDGSLSADMVEKLNASERRIFDQFDVLFRKMNLPEPVRPLSHA
                     FFAALNGVLITFRNYPGRSAKAVHQHMHNIVRVIVSKFCVPSA"
     gene            20111..21700
                     /locus_tag="PLR13_06415"
     CDS             20111..21700
                     /locus_tag="PLR13_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19006.1"
                     /translation="MSLSDRNNPYTFDDFLKWRQGVDYYRDDPFIQKVVHHFTGDIRQ
                     QVDAEARACSQKASFRWRDFADSIARPEKRPFMLHYDGHHNRIDRIVRPHETEIMEKE
                     IFSEAFFADTTSPWVKLIKMYVIYQNGEACIGCPVTCTEGLVMLLEKYADSPETKEIL
                     KHCKDGINGDFAIGAQYLSEIQGGSDVPANVLEAVNENGTWKLYGTKFFCSATHADYA
                     VVTAKPVGSEKVALFVVPSWLPGNKEKEMRNGYTVDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRAVREAKKYTEFRNAFGLTIGQF
                     PLVAVQVNRIDRMAKRTTAGAFKLYRDFLTLEGGLKGGLATDEPEAVRKNRFNIRELI
                     MLQKMAAAWDCTDVIRESMSIFGGHGVMEDFSSLPRLYRDSAINELWEGPRNVLLTQI
                     HRDLQKVSGWYKPAEFVRHILSGLNESTIKELADEITELVAYPNLFQPDEKTMDICRR
                     WDAFCHRFFHAYQDLALKEVE"
     gene            complement(21826..22794)
                     /locus_tag="PLR13_06420"
     CDS             complement(21826..22794)
                     /locus_tag="PLR13_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19007.1"
                     /translation="MIAVQGYPMTLSVLLFIAGIVLLWAGTEFVLQRVPILAGWLRVS
                     PLVVTVLLLAVMTSLPEFCVSLFASMRGQPAAAVGNVVGSNFVTLTFVAGICALWRPI
                     IVGPTLRERESSWMILSAALLLVIAMDGVISRIDGLILLMAYGPYLKGTIDEARQQRK
                     EAPVVSMAKPWLHGIIFVAGIAMIVLGSSWIVENGTMLARRLGMNDLLIGVTFYAFGT
                     SLPELAIALGAVLRRQADVTLGEIYASNIFTGLAVIGCLCLIKPLPVEPIIVQRDLPM
                     LIVAGVLLQMFVTTGRKFVRLEALVMIAIYVLFLAAQFMGFSISLS"
     gene            complement(22865..24199)
                     /locus_tag="PLR13_06425"
     CDS             complement(22865..24199)
                     /locus_tag="PLR13_06425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQC19008.1"
                     /translation="MRFEISRGGSGLTYEIRNIVLIANKLKEYGVDIIWENIGDPVQK
                     GEKIPDWMKEVLFEIIKDDESFAYSPTKGVNATREFLTERLNARGNVRITPEDIIFFN
                     GLGDAIARAYSSIQVDARMIMPEPTYSTHFMAEVLHASFPPNTYRLNPYNGWHPDMRE
                     LEQKVRSHKAIVGILVINPDNPTGFVYPEETLRNIVRIAKENDLFVVFDETYINIVYN
                     GKNTVPLSDIVGSVPAISMKGISKELPWPGSRCGWMEVYNAGKDPIFDRFINTILHQK
                     MSEVCSTTLPQLAIPKIMTHPEYDKYLQERRRHYEKLSDIAYNILKDVPCMMVNKTDG
                     AFYMTIVFNESGLNSNQKLHIKNGEIREFVENLVKEPIEHDKRFVYYLLAATGICVVP
                     LTSFFTPLLGFRMTLLDKDVDEFEHTVKTIAEKFTEYIDSSSKGMQTAIKLY"
     gene            24324..25487
                     /gene="rpsA"
                     /locus_tag="PLR13_06430"
     CDS             24324..25487
                     /gene="rpsA"
                     /locus_tag="PLR13_06430"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQC19009.1"
                     /translation="MTEEKSFSEMLDETSLKPRRFSAGEKIDTIIVKITEEWMFLDLG
                     AKSEGYLDKKELLDENGNLTVKEGDPITAYFVSSRHGEKLFTTKLLTSKSVDDFMFKA
                     YKNQIPVEATVEKEIKGGFSVRINQNVSGFCPYSQMDVRRIDDVTAYVGKKFEFIVAE
                     YSEKGRNIILSRRPLLEKIEQEKKAALKNSLQQGMTVSGVVTSVQKFGAFVDLGGVQA
                     LMPVSEMGWGRVEDPKVLYSPGDKIEAVIINLDWEANRITLSAKASLPNPWDGFVRKY
                     TEGSLLKGKVTNLTNFGAFVSLEDGVEGLLHISKLARGKKIKHAGDVMKTGEELEVKI
                     EKIDRENRKISLDLTGSDKDTSAASGNEDDFRGYIGKSPKTMGTLGDLIKKKK"
     gene            complement(25622..>26308)
                     /locus_tag="PLR13_06435"
     CDS             complement(25622..>26308)
                     /locus_tag="PLR13_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19010.1"
                     /translation="DYVNVLINCRKAIYVEFETDYDISLYGDEREKIMGTMAIMLTGM
                     KAPFYVCNKKYIDEYVKEPTDYIVYDHDRLDMDLLRAGISRVDYWNVWRLTPSLYRKN
                     KESDWIVKKDFQKLDEEGIKDRAEYVLDSSINLILTKHLDIKRSKSPDYKKYYVSLKN
                     ESVPIYEKADNKSKVVGTVPANCKKVFVDSKVSALNGKGIFWHVVHVSVEDNFYLWGY
                     IDNDDIINSN"
BASE COUNT         6797 a         6489 c         6402 g         6620 t
ORIGIN      
        1 tgatctgttg cctcctttag gaaatttttt tctttgtctt aatctaatga aaagaccgtg
       61 ccaaaaaata attttttatt gtattatgcg tttatattat tgaattaatt gattaatatt
      121 atacgcggcg gccggcaaat caacggggaa gggagcccat ttgctcctgt tctttaaata
      181 aaacattaac cactattgaa cgaaatgtat aaatttataa aaaaaccgct tttttgattc
      241 atatttgaca aaaaacggat ttgctgtcta aaaaacaatg gaattgaaaa ggcaaacgat
      301 ggtgaaagtc ccatgaaaga aatatctctg aagacgttgt cgcgggtgga gatgatcgac
      361 atcaccgcct ctgttcaaga ggccgtccgc gatgaaggcg tacagtccgg tgtttgtctg
      421 gtctacaccc cgcataccac ggccgcaatc accatcaatg aaaacgccga tccggatgtg
      481 caacgggaca tgattgcagc gctggacagg gcagtgccgc tttccgcgaa ttaccgtcac
      541 gcggaaggca attccgcagc gcacgtaaaa tcttcccttg tcggagcttc cgaactggtc
      601 atcattgaaa acggacatct ggtgctggga acctggcagt ccgttttttt ctgcgaattc
      661 gacggcccga gaacaagaaa agtttttatc tcaatgattg ccggttaacc ttaacggtgt
      721 gattcaataa accgatattt ttaaaacttc ctatcaaaaa acgaagggtt gccttacaag
      781 acggctatct tagcaaccgc gcataagttg atccgtatta cttaaaccat gttcacacag
      841 cgaacaactt gttgtccgaa actaccttca tgatggttga ctgaaatcaa ccaatcttaa
      901 ctgtccaaac aggcaagaat taaatccgta acaaatacag ttcacatcag actaatacag
      961 gcgcagctct tgtattcatg aagagtcatg cttatattat aaatacgaga tactgcgatg
     1021 cccgtattga gtctattcta ctggaagtta acaaaaaaga agtaaaagag tctttgtggt
     1081 gattatcacc cgtaaaagac atagaattag aatgatggac tccagtaaaa tgacaagacg
     1141 gcatatgcgg gtctcggttt tttttgccag gaggaattaa aaatggggaa aggattaaag
     1201 ggattcagag attacatgga ttatttgact tatgctgaaa tatatttatt ctggatagac
     1261 gggaatgaac ataaaaaaac ttacaatatg ctcatgaaaa ggcagggaga tatagaacct
     1321 attcgttata tgcgtaaaat gagtctggcg gctcacagtc agaattaaat tcgggagcaa
     1381 ataaacaacg ggagagatga gttaaaatgt gggaagccat aagagctatc ggcagtgata
     1441 tcaaaagtga ccgatataaa gtcgccgggg tatggattgt caggacattt aaatcagatg
     1501 aatatgacag cccggagaat aggacttttg tgagtgaccc aaatcacaaa tggaagatgt
     1561 atatgaagga atcatttaac ccttaaatag agtttcttta aagaaaggga aaattaaaaa
     1621 tgtataatat tgcttttgaa acaggtccgc ttttcaatag tatgttccat tttttaggcg
     1681 taaacgcggg tatggaaact tgtcgaaatt tcgggtatgt tattgctgtt attttcggat
     1741 tatctattat aaacattatg ggtaaggctt cctattgtga agttaaagac aaatattaaa
     1801 gatagaggac atatcatggg agacaaaggc ggcaaaaaaa ataaagaaaa aagtcataag
     1861 cagaacactg aaaaacagaa acagaaggaa aaaagcaagt ttgataagca acccaaaagg
     1921 aagccttgat cttgtaatgc caaaagtaat atttagcccg tgaggtaacg cgttgacaaa
     1981 aattcgtgaa gcagctgaaa aaggaattgc ctattcacaa tatgttttgg gcgaaatgta
     2041 ctataagggt aaaggtattc cacaagattc tgatgaggcc gtgaaatggt ttcgcaaggc
     2101 ggcaaaacaa ggtgaagtat atgcccagta taatctgggc tggatatacg gaaccggcac
     2161 aggcgttcca caggattatg ccgaggccag gaaatggtat cgccgggcgg ctgaacaagg
     2221 taacgcaagt gcgcagttga gactgaatga gatgaaagat aaataattga agtagcggaa
     2281 cccaaccgat actttatacg ttatgattgt ttttctcgtt ttccgattcg tacaacattt
     2341 caatctaata aataccattc agacctgaca agaatagtag gaggagagaa tttatgatca
     2401 aaaaacgcaa gaccaagctg gtcatcacta tgggaccagc tttgcaagac agatatctcc
     2461 tgcgggaagc cctgaaactc gccgacgccg ttcggctcaa cgccagtcac agtaggcccg
     2521 aggaacgcac accaatccta aaactcatca gaaatatttc ggacgacctg ggaagaataa
     2581 taccagtctt tctggatctc cagggaccca aatggcgcat cggtctgctg gaagctccgg
     2641 taaacctgga gaaagacagc atcggcgtcc tttacttagc cggcacacca gcgcccgaag
     2701 gttatccatg ggtggcgccg ctgccgcatc aggaattgtt cacaggtgcc aaggtcggtc
     2761 agatctgggt gctggacgac ggggcactga aacttgacgt catggaaaca catcatcagc
     2821 aaatcatgct gaaggtgctg gtcggcggta tgctgaaagc ccgcaagggc gtccatccca
     2881 tcggattgga cgtagccttc gatcccctga ctcctcagga tctggaagat gtccgctggg
     2941 gcgttcagga gggcgtggat ttgtttgcgc agagtttcgt gcgccgtgct tccgacgtgg
     3001 aggcgctgaa tcttcacatc aaggagctgg gcggtccgca aaccattatc gccaaaatcg
     3061 agcatcctca ggcgctggac aatttggagg agattctgca cgtgagctgg ggcgtcatgg
     3121 tagcgcgagg cgatctgggg gttgagtttg gcgtggagaa ggtgcccgcg ctccagaagc
     3181 aaatcatcag gaaggcacgc caggctttga aaccagttat taccgcgacc cagatgctgg
     3241 agagcatgat cgagaatcca caacccacac gggctgaggc gagtgacgtc gccaacgcca
     3301 tatgggacgg caccgacgcc gtgatgctct ccgccgaaag cgccgtcggt aaacatcccc
     3361 tcgaggccgt gcgctatctg cacagcattg ccgcagacgc ggacgcccat tacaaacccc
     3421 gcatcggtct actggctgac acacttgagg aaaatctctc cagccgcacg gacgtatccg
     3481 tagcgttcgc agcctgccgc acggcagaag aaatcggcgc gaagctcatt gtcgttttca
     3541 ccgaaggtgg aggcagcgcg cgcctggtct cccgattggc agcggatatt cccgtcatcg
     3601 gtgccacgac ggacatcgga aattcccggc ggatgggtct tcttcgaggc gtggagagcc
     3661 tgctcattcc tcgggcaaag catctctcgg aaatgttggc ctccatccaa ccgctcctta
     3721 agattagaaa ggggcttgaa tccggtgatc gagtcgtcat gacgctcggc cacccgctgt
     3781 ggactacagg aacaacaaat atgatgaggg tggaaacata ctgatatcag tgcttattgc
     3841 ttgtgtttca caaaagaaaa caaagggaag gctaattggt caagccatgg acttgttttg
     3901 gacacatggc aatctatttc tttctgcaaa tttgacgggc cgcagacgcg gaacgtcatc
     3961 ataaaggttt tgcgtgatta aatgccagcc gttgagattc aagaccatat acgggttgcc
     4021 cggcgcgaac cgcttttaaa aacttaaccg ccgagcggct tcatccttaa aacatcaagc
     4081 atctttttat gaaccaggcc gttggacgca agcacgcctg gcgacgcgat gtcccatttt
     4141 ttaccggaca gatcggaaat cctgccgcct gcctcctcca ccatcagaca gcctgccgcc
     4201 gtatcccagg gtttgagttt gagttcccag aatccgtcga atcgtccggc ggccagatag
     4261 gcaagatcca gtgccgccgc cccggcgcgg cgaatcgcct ggaccttgac ggccatccgg
     4321 ttgaaataat cgagattatt gtcccggctt tcccggatat cataggggaa ccccgtagcc
     4381 agaagactgc gggaaatatc ccggatagag gaaaccgaca gtttcttgtt gttcagataa
     4441 gcccccttgc cgcgcacagc agaaaataaa tcgtctctca tggggtcata aataacgcca
     4501 agaacgacgt tgccgtcgct ttccagcgca atggaaacgc aaaagacagg atatccgtgt
     4561 gcgtaatttg tggttccgtc cagaggatca acaatccagc gtttttttcc ggcgccggca
     4621 atggccggag attcctccga caagattcca tgttccggaa aattccgtct gatggatttt
     4681 ataatcaaat gctcggacat cttgtccgct tccgtcacaa tattgatatc gcccttgtat
     4741 tgaatcttgt gggttttgtt gaatttcttt ttcagtagcg ctccggcgtt tcgcgccatc
     4801 tcgacggcaa acgattttaa ttcccgcata aaaaatactc ctgtcccgtt ttcttcggca
     4861 tactaacaat aaaatcagaa aatattaaag taataattgt ttttatttta aatagttgta
     4921 ttacaatcaa attaatgcta atacacaaac aaaaaaatgg cttcatctgt gaatctccat
     4981 gtttatcgaa cagacaccga gcgacaactc gcaaaaactg aagatgaaga gagagtctct
     5041 ggggctttcg cctgatgatg tatttaaaaa aatacgcatc agaacccgtt atattgaagc
     5101 catagaaaat agcgagtttg accagctgcc ggatccggtt tatacaaaaa attttattaa
     5161 aacctatgcg cggtttctca acatcgacga aaatcccgtt ttgcaggatt acgacgccta
     5221 cctgaatgca caccgcgaag agcagacgcc cccgccggag cccccgccgg agacaaaatc
     5281 tttttttgcc tcatttgccg ccaggaaaaa ttactggggg atcttgctcg tcctgatcat
     5341 ttttatcagc atctggctga ttctcaaaca gaatcagtct gtccctgaag atatcaacac
     5401 gccggcggca gccacacggg ctcccgaagc aacgcttcct gccgccccgg cattaaccga
     5461 cagccagacg cccgcgactg tccccgcgcc gcaaaccggc gtgacggaca atccgccggc
     5521 gacaaccgat gcaaaaaaaa gtgttttatt gatcacggcc ggtcaggaaa cgtggcttcg
     5581 cataaaaccg gacaacagtc cgcctgttca acttctgtta aaaccgggag aaaaatttga
     5641 aagcagtgag gaatttttta atctggatat tggcaatgcc ggaggaatta agatacaata
     5701 caaaggcagg tctatagaga acctggggaa accgggagag gtgatccata taagattgcc
     5761 gcaagtcaaa caataaaaat caagaggtag ggtatgttcg gaatgggatg gcaggaactc
     5821 gttattgttt taatcattgt tgtgattatt tttggtgtcg ggaaattgcc ggaaatcggt
     5881 tcggctctgg gcagaggcat caagaacttc aaaaaatcca tcaatgagca aaacgaaatc
     5941 accgacaatt catcaaaaaa ggaagaagat aaaaaataac tgctgacgcg ataagcatca
     6001 taacccgttt ttcatccggt tatgatgctc gcgcagatat ccttcccggc ttttgcattc
     6061 attttttcca gtgcgcaaac catggtgttt ttatcgcgca agatgcggac gaacggcgtc
     6121 aaacaatgcg tttccggcac gcggcaagct tgcgacgttt atcccggaat tatcttccac
     6181 cggcaacggt cttaatgatg cccagaaaac cctcggcctt taatgacgct ccgccgacca
     6241 gcgcaccgtc gatatcctcc atggcgatta aatcgccgat attgtcccgg gtaacgctgc
     6301 cgccgtagag gatgctgatt tttccggcca cgtcgccgta ggactccttt aaaataccgc
     6361 ggataaagga atggacgtcc tgggcctgcg cgggcgtggc cacttttccg gttccgatgg
     6421 cccagaccgg ttcgtacgcg atcacaatag ggtcgatttt ttcaacacct ttcaggccgt
     6481 gattaacctg cctgccgacc acatcccgtg taatgccctt ttctctttct tcatcggtct
     6541 ctcccacgca catgatcggg atgagcccgt cggacaaggc cttgttcact tttaaattga
     6601 catcggcatt ctgctcatga aaatatttcc ggcgttcgga atgaccgatg atgacatacc
     6661 ggcagccgat atctttgagc atcaacgggg acacttcccc ggtaaatgca cccttgtctt
     6721 cataatgcat gttttgcgcc gccagttcca gacaggagcc tttgatcgcc tcgcccacag
     6781 cggaaagcgc ggtaaaaggc ggagccagga tgacagctcc gccctgaatg tctgatgatc
     6841 cgtcttgaat ggctttcgca agcatgatgg attcggcaat cgtgttatgc attttccagt
     6901 tgccggcaac aatccatttt ctcatcgtta tgtcctcacg atcttgtttg ggtcctgata
     6961 accgttattt ttccaatgca gcaatgccgg gcagcgtttt gccttccagc cattccaaaa
     7021 acgcgccgcc gccggtggag atataagaca ttttcgacgt cagaccggct ttcttaatgg
     7081 cggtatccgt gtcaccgccg ccgaggatgg atagcgctcc ggaatccgcc accgcctgag
     7141 ccagctgaaa cgttcctttg ctgaaagggg caagttcaaa catccccatg ggaccattcc
     7201 agatgaccgt ctttacgcct ttcaaggcct cgctgaacaa tgcgatggtt gccggaccga
     7261 tatccaggcc gatccattct ttgggaatat cctgcacacc gcagatcctg gcttcggctt
     7321 gagcctcggc ggcctgtgca atgacggcat ccaccggcag cagaaactgt acgttcttct
     7381 gtttcgcctt gtccacgatc tttcgcgcgg tatccagcat atcttcctcg cagagcgact
     7441 tgccggtttc atatcccgcc gctctcaaaa aggtaaaggc cattccgccg ccgatgatga
     7501 ttttgtccac tttttcgatc aggttttcca acagaccgat tttgtcggaa acctttgccc
     7561 cgccgatgat cgccgccagc ggtttggccg gattcaccat ggcttttttg aaatattcaa
     7621 cttcgttctt caacagaaaa ccggcggcac atacgggaac aaattcggta attgccgaat
     7681 tggaagccgc cgcccgatgc gatacggcga aggcgtcgtc gatatagacg tcgcaaagcg
     7741 ccgccagttg tttggcgaat tgagcatcat ttttatcttc gcccggctga aaccggagat
     7801 tctccaaaag gataatctgg ccatcctgaa gcctggccac accctgctcc acaaccggtc
     7861 ccacgcaatc ctccaggaag aggacctctt tcttcagaag atccgaaaga accttgacga
     7921 caggcttgag tgacatctcg gcgactcttt tgcctttcgg cctgccgaga tgggaggcga
     7981 cgatcaattt cgcacccttt tccaacgcgt aggtgatggt ggggatactg gctcgcacgc
     8041 gtttgtcgct ggtcacgttt ccttctttat ccatggggac attgaaatcc acccgtaaaa
     8101 aaactctttt gcctttcatg tcgatctgat caatgtactt catcttgaac accttcccta
     8161 tttcattata aacgagagca gttccagcat tctgttggaa aagccccatt cattatcata
     8221 ccaggagaga atcttgacca tgttgccgcc gataaccgtc gtgttgggca tatccacaat
     8281 ggatgaatgc ttgttgccgt tgaaatcttt ggaaaccagc tcttcttccg aacacgcaag
     8341 gatacccttc atggcgcttt gggcatattc acggaattta tcattgattt catttcttgt
     8401 ggtacttttg gaaagtgttg ccacaaaatc cacaagcgaa acattcggcg tcggcacgcg
     8461 aatggccaga ccgtcgagtt ttcccttcat ctcaggaatg acttccgcta tggcccgggc
     8521 cgcaccggtg gtggtgggta tcatggaaag agccgccgcg cgtgctcttc gtaaatctct
     8581 gtgaggttca tccaccacaa cctgatcatt tgtgaaagaa tgaatggtcg tcatcagacc
     8641 gtattcgatg ccgaattcct gatgcagtat tttgacgatc ggcgcaaggc agttcgtggt
     8701 acaggacccc atcgaaatga tatgatgctt gtttttatcg tatacttcct gattgacccc
     8761 cagaacaaac gtcacgtcag gatttttagc aggcgccgac acgacaactt tttttgcccc
     8821 tgcctccatg tgttttccgg cgccggtttt atcggtgaat ttccccgtgc tttccagaac
     8881 aacatcaacg ccgagatcct tccagggcag cgtctccggt tctcggacgg aaaaagtttt
     8941 cagttctttt ccgtctacga taatcgcgtt gtttgcgacg ctaatgtcgg catcgagtat
     9001 cccgtgtgtg gaatcatatt tcaaaaggtg cgccagcgtt ttggcgtcgg caagatcgtt
     9061 taccgcaaca aagtcaacat ctttgttttg atatccggcc ctgaaaacaa gccgcccgat
     9121 tcttccaaaa ccgttaatgg caatcttcac tgacatagta tgacctccct tcatgtctca
     9181 aaaattatta attaaaaggg ggggctttgt caacctgttt tgccgaagcc gcaagcgaaa
     9241 agagcatggc attcgagatg attctctaaa gaaaaaggat agttacgatt cgcagccggt
     9301 cggtattatt ttatgtaatg aataaatcgt tagtggaaaa attcggatcc gttgtcaggt
     9361 tgatgccgag gcggcgcaac cctgcttcat cgccgggtgt gggaatgtgc gtcatgtgcg
     9421 cttcgcagtc cctcagctcc ctgagttttt ccagcgccag ttgcgcggcg ggattatttg
     9481 tcgcgctgat gctgatagcg atcagagctt cctccagatc aaggctcacc gatttttcat
     9541 cgagaatctc cgttttcaga tttttgaccg aatcggtgat ctggggcggc agcaatttaa
     9601 tcttatccgg aatttccgcc agatgcttga tggcatgcaa caccatgctg gtggcagcgt
     9661 gcatcagtga tgagttgctg ccggtgatga tcttgccgtt cttcaattcc agcgctgcgc
     9721 cgcagaagat gccctcgttt ccgcgttcgt aatcccgcgt ttccacggga gcggttacgg
     9781 cagcctcgcg cgccggttga acaaccggac gctcctccgg ctccagatta aaatccttca
     9841 tgaaaagttc cacgcgctgc acggtttcgc gatcggcgaa tcccatggcg tattcacagc
     9901 ggtaacggaa ataacgacgg ataatttcca tcttggccgc cttttgcgtg atcgcgtcat
     9961 cgataatggc aaacccggcg cggttgaccc ccatatccgt cggcgatttg taaaaagacg
    10021 gttcgcccgt aattttttcc agaattcttt tcaggacggg aaaaacttcc acgtcgcggt
    10081 tataattgac ggatttttcg ctgtacgcgt caagatgaaa cggatcgatc agattgaaat
    10141 cccggatatc ggcggtggcc gcctcatagg ccacattcac aggatgcttt aatggaatgt
    10201 tccagatggg aaatgtttca aatttggcgt aaccggattt aatgccgcgc cggtaatcgt
    10261 gataaagctg cgaaagacag gttgccattt ttccgctgcc cggccccgga ccggtcacaa
    10321 tcaccagcgg tttgtctgtt tcgatatagg tatttgcgcc gtatccttca tcgctgacaa
    10381 tggtctccac atccgtcgga tagcctttgg taaagcggtg cgtataaacg gaaataccgc
    10441 gtctttccag tttgttttta aagatgatcg cggcgggttg ttcttcaaaa cgggtaatga
    10501 ccacgcccag cacattgatt ccccaggttc gcaggttgtc aataagcttt aaagcgtctg
    10561 cgtcataggt aatgccgaaa tcggcgcgga ttttttttct ttcgatatcc ccggcataaa
    10621 tgcaaagcac aatgtcggcc ttgtctttta attcccggat gaggcgcatc ttgacgttgg
    10681 gatcataacc cggcaggacg cgggcggcat gatagtcgta taaaagtttg ccgccgaatt
    10741 ccagatacag tttgttgttg aagcggttga cgcgtttgag gatttcggtg gtctgttcct
    10801 ggatgtattt ttcgttatcg aaaccgggct gttgaatcat ctgagttttt ctcctgacat
    10861 aaaattgatc tatcccgaaa acgtttcatt tttgaactat aagtagaaca attttgtaag
    10921 tcaataacta atatcatgtc caacgtgcgc cgtggggatc ggttgcgatc cgctttgcgc
    10981 gatcacgccc cgcgccgatc aataaggctt cgacgcactt atgatttgat gttttaacct
    11041 caattccgca cccgcaagcc gggggtatct gctttgcgga agaggacatc gcttacgcac
    11101 ggttcatgca caaaatagag aggatattgc taaagaaatg caggcgcact gttatgggtg
    11161 cgcctgcata aatgacaaat tcgttatggc aatcggtgcg acaaattatt ttttctgcgc
    11221 gtcataaatt ttgatgattt tgtccgtcac atcaaagccg tcgtcaacaa ccagaaccgc
    11281 tttcttttcg aaaatcatgg agtatttttc gtttttggaa aattgtttca gaatctcctg
    11341 aacgtccatg agaatttttc gggtcagttc ggcatctttc ttcttcaatt gttcattgag
    11401 atcgctttcc agccgtttga actccttgaa ttcctgcgcc agcttctccc gtttttcctt
    11461 gagagccgga gaatcctgtt tcgtgttgct caaatctttt tccagagagg aaactctgtc
    11521 gcccttcgct ttgagtgtcg cttttttgga ctcaaaatcc ttctgataga cagcccgggc
    11581 gttctgggcg gctctggagt ctctcatgat tttcgcgata tccacaaacc ctattttggc
    11641 cgatacggca tccgccaggg cggcaccggc tgagcttatg acaaacaaca gcgcaaaaac
    11701 aaacgccaac gatcctacaa aacgcttatc cttttttttc ataccctatg ctcctttaca
    11761 cgataaagtg ttatcgacct aaatatatgt tccgaagtcc ggttcataca tcgttgcccg
    11821 gatatttccg cgcagatcag caggcgcctg catccgggtc agcgaacgtt taaaaacgac
    11881 ttcggccacg gccagtgcaa ttttggcgga tacctcgcga attgttgtca acgagggata
    11941 gatgcggccg attttcagat cgtcagcaga cacctgttcg ttcagggatt tggcggcggc
    12001 tgtaaacatc tcatccgtca cgcgcacggc ttcggaggtc agcacaccca gtccgactcc
    12061 cggaaaaatg tacgcgttgt tggcctggcc ggggacaaac gttttcccct ccatgctaac
    12121 ttcgccaaaa ggactgccgc tggcaaaaat cgccctccct tcggtgtgac gataagccgc
    12181 ttcggcggta cactcggcct tggaggtcgg attcgacagg gcaaaaatga tcggccgcct
    12241 gtttatccgg cccatcgcct tgataatttc cggtgtaaag gttccgggct gacccgatac
    12301 cccgatgacc gcggtcggct tcagggcttc caccgcggac aaaagatcgc gatggaaggg
    12361 gtaatcatgc gcgtacggtt ttttgtgttc caccagatcg gtgcggcctt tgacaaccag
    12421 tcctttggaa tccacgaacc agcagtttga atgcgcttcc ggcagcgaca ggccttcttc
    12481 catcatggcc gaagaaacca gatctccgat accgatggcg gcttccccgg cgcccaaaaa
    12541 gagcagtcgc tgccgggaaa tactgccgcc ggttatacgc agggcggcat acagacccgc
    12601 cagcgcaacg gacgccgtgc cctggatatc gtcgttgaac atacagtatt tgctgcgata
    12661 tttttcgagc agacggaaag cgttggcatt gccgaaatct tccatctgaa tcatcacgcc
    12721 gggaaaaacg ttcgccgcgg cggccatgaa ttcgtcgacc aactgatcgt attcccgtcc
    12781 gcgcacccgg ggctgcggca gaccgatgta gtccggcgca gccagcaact ccggattgtt
    12841 ggtaccgaca tcaagggtaa ccggcaggca ggcttccgga tcaatacccg cgcaggcggc
    12901 gtaaagcgcc agtttgccga cgggaattcc catcccgttg gcgcccagat cgcccaagcc
    12961 caggatgcgt tcaccgtcgg taacaacaat gacccggacc tttttctgcg gccagcgttt
    13021 caggatcttt tttatgctgc cgcggtcatt tttggaaatg aacagtccgc gaggttcgct
    13081 gaaaatgctg ctgtactcct ggcaggcctg cccgacggtc ggcgtataaa tcacgggcat
    13141 caattcctgt atgtgctcaa tcaacacccg gtagaaaaga cgctcgttgc ggttttgcag
    13201 accggaaagg taaagatatt tttccagatc ggttgttttg cgccggacat tgttgatgat
    13261 atggaccgtc cggtcggacg gcgaaaaaac acggggcgga agcaaaccac gcaaccccag
    13321 gacgtcgcgc tccgtgtcac tgaacatcgt ccctttgttt aaaccgggat cgcaaagcag
    13381 cgccttgccc cggggaattt ttacagcgcg tttttgcgtt gaccggtttg ccattgtttt
    13441 tctcctgaat cgatgaccga atcgttttgg tttacggggt tgacctataa gaagaatgat
    13501 tgcgtgtcaa tataatattc aacaggtttc ccctttgcgt tgacgcggga catgcacgtc
    13561 cggggatcaa atatcttctc ggaatatttc ttgacataca aaacagaaag ttcttatact
    13621 ccgccacaca gtaacttatc aacaatacca acaaaattca aaaacggagg caatgagtat
    13681 ggcagaaaag aaaggattga gcaaaccggt caagctgaag ggggatctcg cggagctttt
    13741 gggcgccaaa gcacttcccc gcacggagat taccaaaaaa ctttgggatt acattaaagc
    13801 caataaactg cagaccacaa aagtcaacgg caaaccggaa aatgccggaa agaacattgt
    13861 gatcgacgcc aaactgatca agatcatcaa caacaccaag gtgaaaacat cgagcggcaa
    13921 ggtcgtggat ttcacaaaat taaaagaagg acaaacgatt gacatgatgc agattgcctc
    13981 tgttgtcagt gccaatgttg agtagacaaa aacattgaaa gacaggcagg ttctatcctg
    14041 cctgtctttt ttatgcccct cctttttctt ctgccgcaac ctttcgccgt accgccggta
    14101 gcgacgcgca cggaattgtc ttttcatttt aaattcatat tatggttttt gaaaaaaata
    14161 ctgtatagtg cagggcgcct gggacacaca ggctccaaaa gactttcaga ttcgggaaac
    14221 gttgacatgg ataaaaaagc cattttgtgg ccggtgatca tatccgtcgt catccacatg
    14281 acgctactgg ccgtggcagg catgatcgat ttaaacgata aaatcaaacc ggtaaacgtg
    14341 ctgtcggtca gcatacaaga accggcgccg gcgcaacctt ctcctgaaaa agaaaaccct
    14401 gcccaaggaa acccgtcgtc cagcgtgacg aagcaacaga acgaccctac gcccgatgat
    14461 gcctggcgcg aagatacaat taacctgggc agtctcgata cgaaatacat tcattacctg
    14521 acccgggtaa aaaacaagat cctccggata tggaaatacc cgcagacgtc cttcgaacga
    14581 aatgaagagg ggaatgttgt cgtcagaatg tccattgatg cggacggcag actggccgcc
    14641 gtaacgctcg tgtcatcgtc gggatcagcc gatctcgata acggggcgct gggcgtcgtt
    14701 caggaagccg cgccctacga acctttgccg ggatcataca atttgtcacg gctgcatatc
    14761 gttgcttctt ttaactacaa gatcatggat tagccgcgtt ccaaaacccc ggacaacggg
    14821 aaaaattccg ctttcatccc gtctgcaaat caggctttat gtcatccatg ttttgattga
    14881 cctgtctgag cagttgaatc acttcctgct gctcggattc gggaagggat tgaattttgt
    14941 ccacccgggc gttgatgtaa tcgatcttcg ctttcagatc ggccagcagc ggagcaagct
    15001 ccagcggcgg tctggacgga cgcggttgtt taatttcctg cggccgggtc cctgctttaa
    15061 tattgatttc ttccagatac tcgggaatgg caacctcaaa tcccagccgt tcgcggatca
    15121 acgaggcaag cgtatcctgc gcaccggctt caccgtggac taaatacacc ggcatttttt
    15181 tattctgaaa atgtcccagc cagtccagaa gctgacgttg cccggcgtgc gcggaaaaac
    15241 cgccgatggt ccatattttg gctttgacgg cgatatcctc gttaaaaata cggatttttt
    15301 tcgcgccctc aacaatctta cggccgggcg ttccctgcgc ctggaatccg acgaacacaa
    15361 tgcttgcgcc gggccgccac aaattatgcc gtagatgatg cctgatccgt ccggcattgc
    15421 acatgcccga tgcggaaatc acaatggccg acccttccat ttcgttgatt ctcattgatt
    15481 gctcggtcga ggacgaaaac tttaactgcg gcagatccag aggatcttca ccatttttca
    15541 gtatgttttg cgtatccgca tccaggtatg tgtggtgccg ccgaaatatc tctgtggcct
    15601 tgatggccag cgggctgtcg agaaacaccg gcatatcctt gggcagcttc cccttgatgt
    15661 ccagcagata aagggaatac agcatctcct gcgttctctc aacggcaaaa gcgggaatga
    15721 tgatcttttc acctctggaa taactgtact gaatggcctc ggccatttca ttgagactgt
    15781 cctcttcgcc tttatgatcg cgatcaccgt aagtggattc cataaaaagg tagtccgcgt
    15841 cgctgatcgt aacgggatcc tccatgagca actgatgacg gcggccgata tcgccggaga
    15901 aaacaagttt tgtggaggtg ccgttttcct caataaacaa ttccaggatg gccgccccca
    15961 aaatatggcc ggcgtcctga aaattgattt tgatgccggc cgcaggcgta aaattctgat
    16021 tgtaggttat tttcttgatc aaaggggaca cggcttcggc gtccggcgtg gaataaagcg
    16081 gctcgctttt ctccgtcctg ccgttacgct gcattcttct cgtcttcgat tttgcatcgg
    16141 tttcctggat atgggcgctg tccagaagca gtatcttgat cagttccccc gtcggctccg
    16201 ttgcgtaaac aggccctttg aatccgttct gcaccatgcg gggcaataat cccgaatgat
    16261 caatgtgagc atgcgtgatc agaaagaaat cgatatgaga cgggtcgtag gcttcagtgt
    16321 cccagttgcg tgtttcaaca tccgcaccgc cctgatgcat cccgcagtca acggcgaatc
    16381 gcgcctgatc ggtttcgata ataaaacagg agccggtgac ttctctggcc gctccgagaa
    16441 atttaacttt catggggaaa tccttttcgt cagttctttc gaggtaaaat ccgtagcggg
    16501 tgaccttccc ggaaaataaa aatgccttta tcccgcatgg aggcgggata aagtgcgctt
    16561 tgtaaagaaa gtcgacaccc gtaaagattt gtcgtttgtg tttatcttac attccggctt
    16621 tcgccgcttt tttcaacttt ttggcttctt ttttttcttt ggcggacttc gccggctttt
    16681 tcttggcgtc ttttttccct ttgtccattg atttactcat ggtttgaacc cccgttaaaa
    16741 gataaaattt cattgttgca tcgtcagttg ctgaatataa acctttacaa ctataataga
    16801 agggccctgt ttcgtcaaac acaaaaatga gggatttctt tttgttgaca acacttggat
    16861 gattaacata agtttttgtg tgctttatgg tgaacagcct atgattgaaa cccgtccgat
    16921 cgaaccggtc atcgtcgccc gcaatctgga aaaagttttc ggcgcgttca aggccgttga
    16981 tgatatttcc ttccatgtgg ttccgggagt ctgtttcggc attttgggac cgaacggcgc
    17041 cggcaagacc tccaccatcc ggatgcttta cggtttctcg ccgctgaccg caggcgacat
    17101 ttttgttttc ggactgaaca tcaaaacaca tgaccggaaa atcaaagccc gcatcggcgt
    17161 ctgccagcag gaaaatacgc tcgaccccga tcttacggtc atgcagaatt tcgaagtgtt
    17221 cgcccgctac tttgatatcg ggaaaaaaac tgccctcgcc cgggcgacaa cgctactgaa
    17281 atttttcgcg ctggaacaac gccaaaatgc ccgcgccacg gaattgtccg gcggcatggt
    17341 gcgccgcctg gtcatcgccc gcgccctgat caatcagccg gatctcatca tcctggatga
    17401 accgacgacg ggccttgatc ctcagtcgcg tcatcaggtc tgggaaagac tggaacaact
    17461 caagaatcag ggcatgacca tcctgctgac cacgcactac atggatgagg cggcgcgtct
    17521 gtgtgataac ctggtcatta tggatcacgg aaaaattctc gcgcagggct ctccccggtc
    17581 gctcatccgg gaatacacgg gcggccatat cattgaggtt gccgaaccgg atgaagcatt
    17641 gcgcgcgttt tcccaagaaa agcacgaccg ggtggaggac ctgggacatc gtttaattat
    17701 ttacgccgcc aacggccatg aactctatca ggagctctgc gaccgtttca gccagaaaag
    17761 ctgcatcctg cgttccgcca cgttggaaga tgtttttttg aaactgaccg gaagggagct
    17821 gcgggaatga ccttcagaat ttccacccga ttcatccgtg tctggcagcg caacctgacg
    17881 gtctaccgag aaagctggaa gatcaacttc atgccgccgc tgctggaacc gcttttctac
    17941 cttttggcgt tcggcatcgg tttcagcgga ctgatcggat cggtgacgta cgccggaacg
    18001 gaaatttcct acgtgatgtt tatcgctccc gcgctgctgg caatcaacat catgaacaac
    18061 tctttctttg aaaacacttt tggatccttt gtgcgcatgt actatcagaa aacgtttgat
    18121 gcgatgatgg ccacgccgct cacggttgac gaaatcgtga ccggagaaat cgtctggggg
    18181 gcgaccaaat cggtcatcgg aacgatcatc atgctgattg tcatcggttt cttcggattg
    18241 atcagctatc cggcaggatt gctgatcgtt cttgtcgcct tcattggcgg ctttgccttc
    18301 ggctccgtgg ccatgatctt caccggcctg gtcggcaaga tcgaattatt caacttgccg
    18361 gtctttctgt tcattacccc gatgttcctt ttcagcggca cgtttttccc gataagcgga
    18421 ttgcccctct gggcgcaata tttcgccttg acgctgccgt tgacgcatct ggtcaacctg
    18481 gtgcgggcct taggcctggg agcgttcaac gccgaagcgc tggccggttt ttgctacctg
    18541 attattttct gtctcatcac ctttccgctc gccctttccg tcatgcgcaa aaaactgatc
    18601 aaataatggt tgccaggaaa aaacgaaata ctgcttgact aaaaattctg agtatgcgca
    18661 acttaatggc ggcgattccg tatatttttt acaaacggat tacaaccatc aaggattatt
    18721 catgtcctga tggttttttc tttaaagatg taaggcagac aagcccctca ctttttcctt
    18781 ccttctcaac caggagacgg atgggggtga catggatgct ttgtaacacc ccatcgggga
    18841 atgaaaaaaa gaaataaaat cagaaagggg ataaagagat gaacaagaat ttatacgtcg
    18901 gcaatctgtc ttataagatc acaggcgatg acctgaaatc caatttttcc gaagcgggag
    18961 aagtggtctc cgcgacgatc atcaaggata aattcagcgg ccagtccaaa ggattcggtt
    19021 ttgtcgagat gaaaacggaa gaaggcgcag ccgaagcaat taaaaaattc cacggcggca
    19081 cgcttgacgg aaaagaaata acggtcaacg aagccagacc gaagaaagag ttcagcggcg
    19141 gccatcgggg aggcggcaac cgcggcggag gaggaggatt caaccggggc ggccgcaggg
    19201 atggaagata ttaatcattc tcgcgttgaa aacgcataaa acaagcagct ttggacctga
    19261 tcccgcattc tagacacatc gggacggcca gaggcgcaaa ataaaaatcc agaggagcag
    19321 aaggcatttt gaaaccttct gctctttttt tggtgtgccc atgaagggag ctatgcagaa
    19381 ggtacgcaaa acttggatac aatgacccgg acaatattat gcatatgctg atgcacggct
    19441 tttgcgcttc ggccgggata gtttctgaaa gtaatcagca caccgttgag cgcagcaaaa
    19501 aatgcatggg acagaggcct gaccggttcc ggcaggttca tttttctgaa caaaacgtcg
    19561 aactgatcaa agatacggcg ttcagaggcg ttgagctttt ccaccatgtc cgcggagagc
    19621 gaaccgtcca gcatgaaacg cgtcatcatg cggaaatact gatcgttatc gatgagataa
    19681 ctcaaatagg catcggccac cttttcgatc gggttttttc tgctgttttt gatcatcctg
    19741 tccagaatgg ccgtaatttc ttccgcgccg tgaagaaagg cttccacaaa aagggattgc
    19801 tgatcgggga aataccggta gatggaggcg tgggaaatgc cggcctcccg ggcaatgtcg
    19861 cgaatgctga ctttgttgaa cggttttacg gcaaagacgc gctgggcagc ttcgacgata
    19921 acgccttgcc gcgcccgttt ttcgttattt ttcagttcgg caaaagtatt tttctttttc
    19981 atatcgattt ttttatacca tatcccgcaa aaacattgca gtaaaaaagc ttgacttatg
    20041 taaccagtgg tttgtaatat aaccgttggt tgagaaagtc aatcacaaat aagattatcc
    20101 gggaggttat atgtctctat ctgatcgcaa caatccctat acgtttgatg attttttaaa
    20161 gtggcggcaa ggcgttgact actaccggga tgatccgttt atccagaagg tggtacacca
    20221 ttttaccgga gacatccggc agcaggttga cgccgaagcg cgcgcctgct ctcaaaaagc
    20281 ctctttccgg tggcgcgatt ttgccgacag cattgcccgg cccgaaaaga ggcccttcat
    20341 gctgcactac gacgggcatc ataaccgcat cgacagaatc gtccgcccgc atgaaacgga
    20401 aatcatggaa aaggaaattt tttccgaagc attctttgcc gacacaacgt cgccctgggt
    20461 caaattaatt aaaatgtacg tcatctacca gaacggcgaa gcctgtatcg gctgccctgt
    20521 cacctgcaca gaggggctgg taatgcttct tgaaaaatat gccgactcgc cggagacgaa
    20581 agaaattctc aaacattgta aagacggcat caacggcgat tttgccatcg gcgcccagta
    20641 tctttcggaa atccagggcg gttcggatgt gccggcaaat gtgctggaag ctgtcaatga
    20701 aaacggcacg tggaaactct atgggacaaa atttttctgc tcggccacac atgcggacta
    20761 tgccgtggtg acggcaaaac ccgtcggatc ggaaaaagtc gccctctttg tcgtgccttc
    20821 ctggcttccg ggcaataagg aaaaagaaat gcgaaacggc tacaccgtcg accggatcaa
    20881 atggaaaatg ggcaccagcg aactgaccac ggcggaactg acgttcaacg gcgcagtcgc
    20941 ttatccagtg ggtcctctgg acaaaggcgt tgccaatgtg gtcggcattg tcctgaccta
    21001 ctcgcgcctg accgtggggc tttccgccgc cgcctacatg acacgggccg tccgggaagc
    21061 gaagaaatac accgaattcc gcaatgcctt cggcctgacc atcggccaat tcccgctggt
    21121 cgccgttcag gtgaacagaa tcgatcgcat ggccaaaagg acaacagctg gagcgtttaa
    21181 actttatcgg gattttctga cgctggaagg aggactcaaa ggcggactgg cgacggatga
    21241 gccggaagcg gtccggaaga atcgtttcaa tatccgggaa ctcatcatgc tgcagaagat
    21301 ggccgccgcc tgggactgca cggatgtcat tcgcgagtcc atgtccattt tcggcggcca
    21361 cggagtgatg gaagatttct catccctgcc tcgtctgtac cgcgactccg ccatcaacga
    21421 actgtgggaa ggtccgcgaa atgttctttt gacacagatt catcgcgacc tgcaaaaagt
    21481 cagcggctgg tataagcctg cggagtttgt gcgtcatatt ctttccggct tgaatgagtc
    21541 aacgattaaa gaattggccg acgagatcac cgaactggtg gcgtacccca atcttttcca
    21601 gccggatgaa aaaaccatgg atatctgccg gcgatgggat gcgttttgcc acaggttttt
    21661 ccacgcctat caggatctgg ctctgaaaga ggtggaataa ttcaccggat tgcttcttaa
    21721 gcggatttgc acaacgctca caattcttaa tggattgcag cttaagcatc gcggatgatc
    21781 agatgtcccg acattatccg aatcggacgt cagcctgaaa cttgatcagg acagagatat
    21841 gctgaaaccc atgaactgcg cggcgagaaa aagcacgtag atggcaatca tcaccagcgc
    21901 ctccagacga acaaattttc ttccggtggt gacaaacatc tgcagaagaa cgcccgccac
    21961 gatcagcatg ggaagatcgc gctgcacgat gatcggttcg accggaagag gcttgatcag
    22021 gcacagacaa ccgatgacgg ccaatccggt aaagatgttg gatgcgtaga tctcgccgag
    22081 cgtcacatcg gcctgacgcc tcaataccgc gcccaaggcg atggcgagtt ccggcagcga
    22141 tgtgccaaac gcataaaagg tcaccccgat gagaagatcg ttcatgccca gccgccgcgc
    22201 aagcattgtt ccattttcca caatccagct cgaccccagc acgatcatcg caatgcccgc
    22261 cacaaatata atgccgtgca gccagggctt tgccatgctg acaacgggag cttcctttct
    22321 ctgctgacgc gcctcatcga tggtgccctt caaatacgga ccgtatgcca tcagcagaat
    22381 caggccgtcg atgcggctga tgactccgtc catggcaatg accagcaaca gagcggcgga
    22441 aagaatcatc cagctggatt ctctttcacg aagggtcgga ccgacgatga tcggtctcca
    22501 cagggcgcaa atgccggcca caaacgtcag ggtcacgaaa ttggagccga caacattgcc
    22561 gaccgcagcg gcaggctgtc cgcgcatgga ggcaaacagt gaaacgcaga actcgggcag
    22621 agacgtcatc accgccagca acagaacggt caccaccagc ggcgataccc gcagccaccc
    22681 cgccagaatg ggaacgcgtt gcaggacgaa ttccgtgccc gcccataaaa gcacaatgcc
    22741 cgcaatgaac aacaaaaccg aaagcgtcat cggatacccc tgaactgcaa tcacgcaaaa
    22801 ctgcccggcc cgtcattgca gattccgccg gatcaaatta aaaggcttgt ctcgcggtca
    22861 ctccttaata taatttgatg gcggtctgca tccctttgct tgaagaatca atatattcgg
    22921 tgaatttttc ggcgatggtt ttgaccgtgt gttcgaattc atccacatct ttgtccagaa
    22981 gcgtcatgcg gaagcctaaa agcggtgtaa agaacgatgt cagcggaaca acacagatcc
    23041 ccgtcgccgc cagcagataa taaacaaatc tcttgtcgtg ttcgatgggt tctttgacga
    23101 gattttcgac gaattccctt atctctccat tttttatatg aagcttttgg ttgctgttga
    23161 gaccggattc gttgaacacg attgtcatat aaaaagcgcc gtctgtttta ttgaccatca
    23221 tgcagggaac atcttttaaa atgttgtagg cgatatccga aagcttttcg taatgcctcc
    23281 tgcgttcctg gaggtattta tcatactccg gatgcgtcat gattttagga atcgccagtt
    23341 gcggcaatgt tgtcgaacaa acctccgaca ttttctgatg gaggatggta ttgataaagc
    23401 ggtcgaagat cggatcttta ccggcattgt aaacttccat ccagccgcac cgtgagccgg
    23461 gccagggaag ttccttggaa attcctttca tgctgatggc cggaacggag cctacgatat
    23521 ccgaaagcgg tacggtgttc ttgccgttgt aaacaatatt gatgtaggtt tcatcaaaga
    23581 caacgaaaag atcgttttct ttggcgatcc gtacaatatt tcgcaacgtt tcttcaggat
    23641 agacaaagcc ggtgggattg tccgggttga tgaccagaat gccgacaatg gctttgtggc
    23701 ttctcacctt ctgttccagt tcgcgcatat ccggatgcca tccgttgtat ggattcagcc
    23761 ggtaggtatt gggcggaaac gacgcgtgca gcacttccgc catgaagtgc gtggaatagg
    23821 tcggctccgg catgatcatg cgggcgtcaa cctgaatgga gctgtaggcg cgggcaatgg
    23881 catcgcccag accgttgaag aaaatgatat cttcgggggt aatccgcaca tttccgcgcg
    23941 cattaagacg ctcggtgaga aactcccggg tggcgttaac acctttcgtc ggggaatagg
    24001 caaaagactc atcatctttg atgatttcaa agaggacttc cttcatccag tcgggaattt
    24061 tttctccctt ttgcaccgga tcgccgatat tttcccagat gatatcaacg ccgtattcct
    24121 tcagtttgtt ggcaatcagt acgatattcc gtatttcata agtcagcccg ctgccgccgc
    24181 gggaaatttc aaaacgcata aatattttaa ccccttagat gatatttgta tttcataata
    24241 catatttgtt taaatttcca gttttttatg ctatcggagc accaaacgaa acgcctgtcg
    24301 ggcgtaaaaa taaagaggac aatatgacgg aagaaaaaag tttttctgaa atgcttgatg
    24361 aaaccagctt aaaaccccgg cgtttttccg ccggagagaa aatagatact atcatcgtaa
    24421 aaattacaga ggaatggatg ttcctggatc tgggagcgaa aagtgaaggc tatctggata
    24481 aaaaggaact attggatgaa aacggcaatc tcaccgtgaa ggaaggagat cccatcacgg
    24541 cttattttgt ctcttctcgt cacggggaaa aactttttac caccaaactg ctgacgagca
    24601 agagtgttga tgattttatg ttcaaagcct ataaaaatca gattcccgtt gaagcgactg
    24661 tcgagaagga aatcaaaggc ggcttttcgg tcaggattaa ccagaatgtc agcgggttct
    24721 gtccctactc gcagatggat gtcagaagaa tagatgatgt cactgcctac gtcggcaaga
    24781 aatttgaatt tattgtggcc gaatattcgg aaaagggtcg caatatcatt ttgtcacgtc
    24841 ggcctttgct ggaaaaaatc gaacaggaga aaaaagccgc gttgaaaaat tccctgcaac
    24901 aggggatgac ggtgagcggc gtagtgacaa gcgttcagaa attcggtgct ttcgttgatc
    24961 tcggcggtgt gcaggccctc atgcctgttt cggaaatggg ctggggacgg gttgaagatc
    25021 ccaaagtact ctactcgccg ggcgacaaga ttgaagccgt gatcatcaat ctggactggg
    25081 aagctaatcg catcacgtta tcggctaaag cctctcttcc caatccgtgg gacggctttg
    25141 tgagaaaata caccgaaggt agcctgctca agggcaaggt aaccaacctg accaatttcg
    25201 gcgcttttgt ttcgctggaa gacggggtgg aagggctttt gcatatttcc aaactggcgc
    25261 gcggcaaaaa aatcaaacac gccggcgatg ttatgaaaac cggcgaagaa ttggaagtga
    25321 agattgaaaa aatcgaccgc gagaacagaa aaatttctct tgatctgacc ggaagcgaca
    25381 aagatacttc agctgcttca ggaaatgaag atgatttccg cggttatatc ggcaaatcac
    25441 ccaagacgat gggcacgctg ggcgacctga tcaagaagaa aaaataaatt ctgggttgaa
    25501 aaagaaaata cctacaagtc cacattttta tagaataaag aaaattgagg attactagtt
    25561 gaaaggtgca acgcctgata tgaggtgtgg cggctttttg ttgttgccat taatgccgtt
    25621 gtcagttact atttataata tcatcattgt caatatatcc ccaaagataa aagttatctt
    25681 ctactgatac gtgaacaaca tgccaaaaaa taccttttcc atttagagct gaaacttttg
    25741 agtcaacaaa tacttttttg cagttggctg gcacagttcc gacaactttg cttttattat
    25801 cagctttttc atatattggg actgattcat tttttaaact cacataatac tttttataat
    25861 ctggtgattt tgaacgtttt atatctaagt gttttgttaa aattaaatta attgaagagt
    25921 cgagaacata ttcagctcta tcttttatac cttcctcatc aagtttttga aaatctttct
    25981 ttacaatcca atctgattct ttgttttttc gatataaaga tggagtaagc cgccaaacat
    26041 tccaataatc gacccggcta attcctgccc ttaacagatc catatctaga cggtcatgat
    26101 catagacaat ataatctgtc ggttctttaa catattcatc gatatatttt ttattgcaaa
    26161 cataaaatgg agctttcatt cccgttagca taatagccat agtacccatt attttttctc
    26221 tttcatctcc atatagactt atgtcatagt ccgtttcaaa ttctacataa atggctttcc
    26281 tgcaattgat aaggacatta acataatc
//
LOCUS       DAONQZ010000029        25065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2190_length_25065_cov_17.678449, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000029 DAONQZ010000000
VERSION     DAONQZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 25065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25065
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_25065_cov_17.678449"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PLR13_06440"
     CDS             <1..1211
                     /locus_tag="PLR13_06440"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQC19011.1"
                     /translation="YPTPFHIYDEKAIRDNARAFINAFNWNEGFKEFFAVKAAPNPYL
                     MKILHKEGYGADCSSLAELMMAQKTGITGEEIMFSSNDTPVEDFLLAKKLKAIINLDD
                     ISHIAFLEKSAGLPALICFRYNPGKLKKGNHIIGHPEEAKYGFTREQLFEGYKICRDK
                     GVRRFGIHTMVASNELDPYYFVETAEILFNLIVELSQKLKIKFEFVNIGGGVGIPYRP
                     EQKSIDVNVMSRGIRKKYNDIIVANGLAPLKLFTECGRYITGPYGYLVSRVRHIKNTY
                     KKFAGLDSCMANLMRPALYGAYHHITVLGKERKSHNILYDVTGSLCENNDKFAIDRKL
                     PKLEQGDILVIHDAGAHGFAMGFNYNGKLRSAELLLRANGKVVQIRRAETIKDYFATL
                     DFNGLKDFNA"
     gene            1373..2932
                     /locus_tag="PLR13_06445"
     CDS             1373..2932
                     /locus_tag="PLR13_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19012.1"
                     /translation="MASFKDIFNAAEQGNPAAQYNLGLIYRKGEAVPQDDAEAVGWFL
                     KAAQQNHAKAQLNLAWMYQEGKGVSQDDVKAAKWYLKAAEQGNAQAQFNLALMYSKGE
                     GVALDHIEAAKWFLESAEQGIANAQYNLGIMYQDGLGVQKDSTEAVKWFNKAAKQGHT
                     DAQAKLDAMRKALQDEQREAARAVKESPRILPSNAADASSIPDEFPDEKKEALSQDHA
                     KQILVSQYLKAAEQGLADAQYHLGVIYEEGSGVPRDNEKAIKWYRAAAEQGHPEAQNK
                     LEALSPAGHETMPGDTQPPVDGNETTAYTKPDESANALIVVTDELAEQNHDIEQEVPE
                     TTATESNPSPEISDDEKGYREAAEQGDADIQNRLGLMYKNGDGVMQNHVEAAKWFLKA
                     SEQGFADAQLNLGNMYQEGSGVPQNDAEAAKWYREAAEKGHAGAQYNLGLKYFFGEGV
                     PRDFAEAIKWYQKAALQDNVDAQFTLGGIYEEGVVIPEDPAEAERWYRRAAEKGHERA
                     RYRLELMHRHV"
     gene            3280..3921
                     /locus_tag="PLR13_06450"
     CDS             3280..3921
                     /locus_tag="PLR13_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19013.1"
                     /translation="MLKISRHIHEKRFPQILTLFFTLILVCLTMVWSADPAYSNDTQD
                     KLYSLNLYYGEISKNHADDFILDTGTLRLTDCRLAAVTLARRLYAYKDLANLEVEGQI
                     VKHFYDIQDHWEFNAIVTARWNAFFWDKYVDTSIAFGLGPSYATEKPEMEAILDRETS
                     QWLAYWMVELEMGLPAHPGISFITRLHHRSDAFRIVSPGGASTAPVVGLKIRF"
     gene            4159..5004
                     /gene="murI"
                     /locus_tag="PLR13_06455"
     CDS             4159..5004
                     /gene="murI"
                     /locus_tag="PLR13_06455"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQC19014.1"
                     /translation="MNTKANRAIGVFDSGIGGLTVVRALMERLPFENIIYFGDTARVP
                     YGVKSVETINRYAAQITEFLIQKDVKMLIVACNTMAAVAHQTIIDLSQVPVAFAYQKL
                     VEQVPVLEVIDASARMAIQHTKTKSIGVIGTPATVNSNAYARAIHLLDKEAKVFSHAC
                     PLFVPLVEEGWFDHPATKLIAQEYLKQVIAEQVDTLVLGCTHYPLLKPLLQDIMGKSV
                     TLIDSAEAMAGIAADLIDQQAIGNQNHAVPEYTFYVSDLPYRFQTIGERFLGRTLGKI
                     ELVSF"
     gene            5014..6477
                     /locus_tag="PLR13_06460"
     CDS             5014..6477
                     /locus_tag="PLR13_06460"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQC19015.1"
                     /translation="MKILLAGHNLDYDIIRTLKDKSGTKQDLTPETISAAYARISRNP
                     KPVNELRAIAREEVDKARQSNRKIVFDMGHNSVAEHAVFNIDVIGVSRFLVEEIEKFR
                     LCSFTEKSQRYVLFDKDFVVPEEIRQADLAGLFISTVEAQNDFYHEMYENLRPYIFDK
                     HKTLASDPANRSMLEGWAKEDARYAISMATETQLGMTVNARNLELMLRRLAALPLAEA
                     QEYSAKLYAATKEIAPSLIRYTKATDYDKFTRRNLNRFSDALIKKSQSKVKNAADVQL
                     MSASAAADNRAAAALLFASSDANYAECLKTADKLTAQKKRSLFKTAFANLQIHDAVLR
                     ELETVDLQFELIASSSCFAQLKRHRMSTIIAQEYNPHLGVTVPPSIKAIGRQKDFLDL
                     MHRTQSTYEKIREKAPLAAVYVLTNAHRKRVLMKLNAREMYHLARLRADAHAQWDIHN
                     LTGKMLKQAKKVMPLTLMMACGKDHFPSLLNKTYSCT"
     gene            6501..7895
                     /locus_tag="PLR13_06465"
     CDS             6501..7895
                     /locus_tag="PLR13_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQC19016.1"
                     /translation="MKINANKNHLYVLLVTTLSSFLTPFMGSAINVALPAIAEELSMT
                     ALTLSWVATSFILAASISLIPLGRLADIYGRRRIFIYGALIFTVASAFCIWSPTQAFL
                     IAVRALQGIGGAMIFSTGTAMLISAYPPSDRGKILGINITAVYVGLTVGPFIGGLLTQ
                     YLGWRYIFLFTVFLGLTIIFITLAKIEPETSAREDEGFDLTGALIYAIALFSIMYGFS
                     HLPAFSAGCLIAIGMIFLGLFIFQQLKSPFPLLDIRLFLDNKVFAFSNLAALINYSAT
                     FVINFLLSLYLQNIKVLTPSEAGSILIAAPIMQALISPFAGRLSDRFEPQIIASIGMS
                     ITVAGLVLLIFLNGSTGIHYVLFCLVLLGIGFALFSSPNVNATMSSVENKFYGIASAT
                     LGTMRLTGQMFSMGITMLIFAVILGNHPISAANHHLFLKSTKLIFSILSILCCAGIFA
                     SLARGKMHEGNHSL"
     gene            7873..8643
                     /locus_tag="PLR13_06470"
     CDS             7873..8643
                     /locus_tag="PLR13_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQC19017.1"
                     /translation="MKAITLSSCIIGLVAGTFGGLIGIGGGIIMVPLMVEILKLPQHK
                     AHGTSLVALVFTGISGSITYALNGSIDIKAAALLALTAIMTASLGARYAGSLTEQKLK
                     KAFGAFLLFCAALLFLKLYLPNVVVSDDAKPVIYLTTGALTGFLSGMMGVGGGTIMIP
                     VMVILAGFTQHVAQGTSLLVMIPSGAVGALTHWKMGNTEQGILWGLIPGILLGTYIGG
                     TVANIIPNDTLRLIFAGMLIFLGIRYFGMSSPRRIGKG"
     gene            8646..9665
                     /locus_tag="PLR13_06475"
     CDS             8646..9665
                     /locus_tag="PLR13_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19018.1"
                     /translation="MKKTDGYRKFSFFTDVRGMKNAIGEMRGWGHPHQPIFNAGYQRL
                     PDDPVFNEDWFISTIKEKIATHPENTMEYPFYGEKIFTEPLIGFIQGDDPIFQQYKTI
                     IGPHHFTPEEIMQWQARQHGVPAPRAEDLSVVSFILPLTKITKNDNAAMKEWSAERWA
                     QTRLLGEIFSQIFVREIVTCLMKRGILAIAPDVTPMFNKKRYPKVGWASPWSHRHMAY
                     AAGLGTFGMHDFFITEKGCAHRTGSFVVNVKLTSNRERPDDIHAHCLQYQGKSCMQCA
                     KRCPVNAITAENGHNKEVCYQRVAKSLIHCNKNYHIFIYGCGLCATGVPCESEIPKIL
                     QKNSK"
     gene            complement(9809..10921)
                     /locus_tag="PLR13_06480"
     CDS             complement(9809..10921)
                     /locus_tag="PLR13_06480"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQC19019.1"
                     /translation="MIKSRLCDMTGIKYPIIQAGMGPFSNNNLGVAAANAGVLGLFST
                     SGLCNKDFLPFIYNDWIKSAEANPDDDIPTAIEKLLKRAYRLTKDTNGVFGINVMVSA
                     ELLFQAELIINAAIQVRAANPGMEKHFKVIFTSAGDPVPWKEKIRGAGFTWMHVVPSV
                     KGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPAVVEAVSDEKTLVCGAGGFCD
                     GKTLAAALVMGADGVQMGTRFLATQESDFPPIWRQGVVEAGDRGTLVARGFVGPARWL
                     KNPRSEEHAQNTLQKSPGCYLGTPDDMLSIDFKLIEFEMESMQAAYDGNKEKALLAAG
                     EAAQRINDMPKVADMVQSMMKETEDILKGVQTKMLA"
     gene            complement(10961..11965)
                     /locus_tag="PLR13_06485"
     CDS             complement(10961..11965)
                     /locus_tag="PLR13_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQC19020.1"
                     /translation="MKKNSKKSIWVFGDYRNYYQNRVTLQLIARAVALAKVINAEVCA
                     LIFGHEIDEWIMEYTAHGADRILAIDDPALKSYSTEKYLAIIERLAGERDPEIILIGA
                     TDFGREFAPRLAKRLKTGLSADCVGLDIADDGLLLQIAPSFGGNMLAEIVTERHRPQM
                     ATVRPGTFKEIPHDNQRKALVERLPLMKNLPKPRVRLIEYERSVEKEHTIENASVIIC
                     GGRGMGNKEKFKKLQELARLLGGDVGATRPVVYAGWADHDALVGQAGKHIKPKLLFSF
                     GISGAIQHTAGLGEAHFIIAINKNPQATMLKMADVAIIGDASDVLNHMIRELKKRIRE
                     "
     gene            complement(11952..12752)
                     /locus_tag="PLR13_06490"
     CDS             complement(11952..12752)
                     /locus_tag="PLR13_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQC19021.1"
                     /translation="MVTELPWDEKTGTLRRDLAAGMMNPACKHALEAALQIKEHHPAN
                     ITVITMGPPAAEEVLREALALGADQAVLVCDRLLAGSDTFATSLILKEAIKKKCPKFD
                     LILCGAYTADSETAQVGPQLAEELDLPAAAYVEKLEISGKTLRVRRLVDNFRETLEME
                     FPALVTISMENYKPRYTGLAGLESAFDKNDVVILDAKALGLTAASIEKKGSRTKVRKV
                     FLRKTQKGNVLLQGAASAVVTEFLDKYQRKIGTVIGKSIEEKKSDEKK"
     gene            complement(12791..13942)
                     /locus_tag="PLR13_06495"
     CDS             complement(12791..13942)
                     /locus_tag="PLR13_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19022.1"
                     /translation="MQLNPCAKHKLIRDSVRTFAETELAPVASAIDRAATFPREIIEK
                     MRPLNYFGLQVPREFGGAALDSVSAAIVVEEISRVCAAIGLCVTVHNGVAVYPFLRFA
                     NAEQKKHYLPKLAEGELIGAFSLTEANAGSDSASVETTAVKRNDAYVLNGTKIFVTNG
                     GVCDLALIFALTGSEQGKPQSSVFIVESKYPGFLRGELEDLCGMRANPVSSLFLEDCC
                     VPENNVLGRIGDGMKIGLATLDNGRIGVAAQALGIAQGAFEAAVKYAKERQQFKKPIA
                     SLQTIQNYIADMATEIAAARLLLYRACSLKDEGMPFGCEASMAKLYCSTVASRVTSLA
                     VQIHGGYGYSKEYDVERYFRDAKVTEIYEGTSEIQRMVIARSILSQLIL"
     gene            14118..15467
                     /locus_tag="PLR13_06500"
     CDS             14118..15467
                     /locus_tag="PLR13_06500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQC19023.1"
                     /translation="MVHREAKNKSSRKTKAVHHDPDALPSLMANPQEKLAVSLPLRLI
                     IRLALGFVDLFHIWRFPRKTDGCFEHMTLIDKLHWLYKSANPIRLAEKKSSLEDFFAG
                     QAETSLTLPPDFEQTTQIQLAAVGDLMQNDLLTHSADSLYEGIAAELFTADISFANLE
                     APLTTQPQRNLLFVRSETPSTYISAGQFQVLKGYKDQKFTVFSTAGNHAWDYGEEGVL
                     STLDALAKEGIVAVGTNREKADRQKAAIIDVQGIRIGFVGLTFGLNGKKIDPEQEYHV
                     NLVQLNALSAEVDLSVPIRQINYCREQYCDFIVGSLHWGLEFEFFPARRQLAVAHQLA
                     EAGLDLIISHHPHVVQPVEFYRTRRDPQRTVVITYSLGNMVSPFHDDDLNLSFLLQVT
                     LADGKLKNQKRTYIAACRIIPVCQRALMAGGKPTVRLEKVTRLAAFRLPFDLKNLAP"
     gene            15642..16142
                     /locus_tag="PLR13_06505"
     CDS             15642..16142
                     /locus_tag="PLR13_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HQC19024.1"
                     /translation="MVIIYPMSKEKIPTTPAVLALKAQQADFTLHTYPYEERGGTKAS
                     ARKLGVDEHMVIKTLVMEDETGKPLIILMHGDREVSTKSLARQIGAKSVTPCKPAVAE
                     KHTGYKVGGTSPFGTRKVLPVYMEQTIADLPDILINAGSRGLLARMPSAELARLLKPV
                     MVGVAI"
     gene            16192..16749
                     /gene="trxC"
                     /locus_tag="PLR13_06510"
     CDS             16192..16749
                     /gene="trxC"
                     /locus_tag="PLR13_06510"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HQC19025.1"
                     /translation="MPDDVIIRCLNCGTKNRIPKQKLQGRSICGKCGASLDELIIRCL
                     KCSTKNRIPEDRLNQRPLCGKCHEPLIIPQQKVLPFDVTDQTFAREVLTSDSSVLVDC
                     WAPWCGPCRTLSPIIDELASDYANGVKVVKLNVDENPLTASQYGIRSIPTMLFFKEGK
                     MVHRVTGALPKEEIEQHLLTIIKTN"
     gene            16765..17301
                     /locus_tag="PLR13_06515"
     CDS             16765..17301
                     /locus_tag="PLR13_06515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQC19026.1"
                     /translation="MADNKSSIVYQCKIFDVREEEVTFPNGKTARQSWINHHPTVAIV
                     AVNEKNELLLIKQYRNAVKQDLLEIPAGSLDGIEESPAVCAQRELAEETGFKAKSLIK
                     LYEGYLLPGYCNEYMYFFLALDLFSAPLNPDDDEFIETIPATFSQAHKLIDRGVIIDA
                     KTVLGILLAEKLLGRQNA"
     gene            17402..18700
                     /locus_tag="PLR13_06520"
     CDS             17402..18700
                     /locus_tag="PLR13_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HQC19027.1"
                     /translation="MIYNEEFETMPREVIKALQVKRLQQVLERVYHTVGFYKKSFDAA
                     KVKPDDIRCMADMKKLPFTTRKDLRDNYPFGLFAVPMSSVVRLHASSGTSRRSAVFGY
                     TKRDIDTWSDLIARSLVAAGITKNDIIHNAFGYGLFTGGLGLHYGAEKIGASVIPISG
                     GDVKRQLMILQDFGPTVLCATPSYALHLAEEGNAMGIDIKALKLRVGIFGAEPWTDAI
                     RDDIEKAFGITALDLFGLSEVIGPGMAMECLEGRHGMHIFEDHFIVETIDPQTGDVLP
                     EGAEGELVFTTLTKEANPLVRYRTGDISRLITEPCRCGRTHIKMERVLKRSDDMLIIR
                     GVNVFPSQIEAILVEIEGLEPNYQVIIDKAGALDVLDLQVEVNDKIFSDSGSIKQLQK
                     IEQRIVKDMRDYLGVSARVKLVQPNTLKKTDAKIIDKRRI"
     gene            18723..19154
                     /locus_tag="PLR13_06525"
     CDS             18723..19154
                     /locus_tag="PLR13_06525"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQC19028.1"
                     /translation="MKVEQISVFLENKPGTLEDATRVLKEANINIRTLSVAETVDFGI
                     LRLIVNDVEKANKVLKDNGFRVSKTPVVAVEVPDTPGGLHSIMEVVSKEGINVEYLYA
                     FVEKSGQNAVIIFRFDNPEKAIDVLTKKNFTVIPGSKLYEF"
     gene            complement(19470..20357)
                     /locus_tag="PLR13_06530"
     CDS             complement(19470..20357)
                     /locus_tag="PLR13_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC19029.1"
                     /translation="MPVEKTRTTHILLSVFHGIPRCVRKGMFLSIFFLFYHIGTKSRL
                     ITLHNLMRSFPEKDTKEIIKIAKGVYRHFAIVVAEFFDLPYMTKDNIHKWVDVEGVEN
                     FQAGIAKGKGMLSIVAHFGNWELMTIAGPMYLKPMYIVYRPLDNHVIDNIVEYVRTMQ
                     GNDMIPKGGSGKRIMELLRENNLIGILSDQNVAHYEGVFVDFFGRTACTGVGLAVMAM
                     RSGAPVLPAFMARQKSGKYKFIIKPTIEAVCTDNYENDLVVNTQRFTKIIEEVIREYP
                     DQYFWFHQRWKTKPWQKKM"
     gene            complement(20370..20672)
                     /locus_tag="PLR13_06535"
     CDS             complement(20370..20672)
                     /locus_tag="PLR13_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19030.1"
                     /translation="MTEIIFDENEDWEKRVLCSDESCIGTIGPDGKCKECGKPYEGKL
                     PEGKINEIVHEVSPEKQVSPDDQADDDWDKRVLCSDGSCIGVIGPDGKCKECGKPL"
     gene            complement(21294..22736)
                     /locus_tag="PLR13_06540"
     CDS             complement(21294..22736)
                     /locus_tag="PLR13_06540"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQC19031.1"
                     /translation="MSDIKLKKLDDNRWLLPQTGGMRVPGIIYANEKLFQLLKGDESA
                     KQVANVAHLPGIVNFSLAMPDVHWGYGFPIGGVAAFDLDGGIISPGGVGYDINCGCRL
                     MTTNLTLGDIKNRLSDLTTALYQRIPSGVGSTGSVKLSGKDQKKVLEEGAAWAIKAGF
                     GLNEDLETTEDGGAISGADPELVSARALERGKQQLGTLGSGNHFLEIEVVQEIFDEEA
                     AKAFGLHKGQIAVMIHTGSRGLGYQICDDYLALMVKHANETGIALPDRQLACAYLSSG
                     RGKNYLAAMACGANYAWANRQILMHKTREVFEKTLRMSPRDVNMRLVYDVCHNIAKIE
                     SFNIGGVIKKLCVHRKGATRAFPAGHEAVPVRYRKVGQPVLIPGDMGTGSYVLVGQQK
                     AMSETFGSACHGAGRVMSRSQAIKNSRGRSIARELSEKGVLVMAGSKGTLSEEMPEAY
                     KNVQDVVQVMHEAGIARKVARLKSLSCIKG"
     gene            complement(22877..23416)
                     /locus_tag="PLR13_06545"
     CDS             complement(22877..23416)
                     /locus_tag="PLR13_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HQC19032.1"
                     /translation="MTVKQSANTYCLTQDQLVMKVAEQSALLVQYARFSYTHSMSNYK
                     FFDHTADIGVEVYGRTRKELFMNTAGALFDVMIEHKAGRATAKRQKKITVEGTDVADL
                     LINFLRELLHLFNGEHFITGSCEIIEFSNKKLQARLTGESFNNKKHSIKTEIKAVTYS
                     GATVKRVESGWKARIIFDV"
     gene            complement(23621..24373)
                     /locus_tag="PLR13_06550"
     CDS             complement(23621..24373)
                     /locus_tag="PLR13_06550"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19033.1"
                     /translation="MPTHIERLEAHASHLLDAFIQLRERYALLEPMLFDESMPKERCS
                     GNQARGFCILKHSLFLSCSQDIANLTLDDDDKTPSLHNLIRAITDDVIRKELQNRFAI
                     WKIPSIEEQTEPEIIEALHRIELREEAERREQFEKLYCEAMAIWAELSTSKKIKAFRI
                     VRDKVSAHKEVRYVADKYQFVDIGTLGIKWGDLRSTIDRMKRLVELMGLLVRNAGFAW
                     DMLDEQLSRASKGFWDISNAAEESIESGERKQ"
     gene            complement(24446..24802)
                     /locus_tag="PLR13_06555"
     CDS             complement(24446..24802)
                     /locus_tag="PLR13_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19034.1"
                     /translation="MNKLRSLFSIKGIVLSCSAFFFCYARHIIQYKNPPWQNGFLGFL
                     GSWFSHYIVILLIAVIVLAIVKNCGRYLFIDKEPHLDNIEDTVIHVSLTILAASLLLL
                     FAYYVGPHVYVDDIYE"
BASE COUNT         6366 a         6590 c         5828 g         6281 t
ORIGIN      
        1 attatccgac gccctttcat atttatgacg aaaaagccat ccgggacaat gcccgcgctt
       61 tcataaacgc cttcaactgg aatgaaggat ttaaagaatt ttttgccgtt aaggctgctc
      121 ccaatcccta tttgatgaaa atccttcaca aggaaggtta tggcgccgat tgcagttcgc
      181 tggccgaatt gatgatggca cagaaaaccg gcattaccgg cgaagaaatc atgttctcct
      241 caaacgatac cccagtggaa gattttctgc tggccaaaaa attaaaggcc atcatcaatc
      301 tggacgatat cagccacatt gcctttttgg aaaaaagtgc gggcttgcct gcactgattt
      361 gtttccgtta caatcccggc aaactgaaaa aaggcaatca tattatcggc cacccggaag
      421 aagccaaata cggtttcacc cgcgaacagc tttttgaagg ctataaaata tgcagggaca
      481 agggcgttcg gcgtttcggc attcatacga tggtcgcttc aaacgaactt gacccttatt
      541 attttgtgga aaccgcggaa atactgttca acctgattgt tgagctttca cagaagctga
      601 aaatcaaatt cgaattcgtc aatatcggcg gtggagtagg aataccctac cggccggagc
      661 aaaaaagcat cgacgtaaac gtaatgagtc gcggcatcag gaaaaaatac aatgatatta
      721 tcgtggccaa cggtctggct cctttaaaac tatttacgga atgcggccgc tacatcaccg
      781 gaccctacgg ttatcttgtt tcccgcgtgc ggcatatcaa aaacacctat aaaaaatttg
      841 ccggactgga ttcctgcatg gccaacctga tgcgcccggc gctttacggc gcctatcatc
      901 acatcaccgt tctgggcaag gagcgcaaat cacacaatat cctttacgac gtcaccggat
      961 cgctttgtga aaacaacgac aaattcgcca ttgaccgcaa gctgcccaaa ctggagcagg
     1021 gcgatattct cgtcatccat gatgccggcg cgcacggttt tgccatgggc ttcaactata
     1081 acgggaaact tcgttcagcc gaactgctac tgagagcaaa tggaaaagtg gtccaaatcc
     1141 gccgggctga aaccataaag gattatttcg caacgctgga ttttaacgga ctgaaagatt
     1201 tcaacgcgta gatataaaag gaatgacgca gatcagtgtt tcactgatct gaacgtgcgt
     1261 caaataaatc gacttccaga cggattgaga cagaggcatg cggacattaa tgatgttacc
     1321 gtcatgtctt ctttaaaatt ttctacaacc atcacaaaac cctttagata ttatggcttc
     1381 attcaaagat atttttaacg cagctgaaca agggaacccc gccgcacagt ataatctggg
     1441 gttaatttac cggaagggcg aagccgtccc gcaggatgac gctgaagccg tcggctggtt
     1501 tctcaaggcc gcccagcaaa atcatgccaa agcccagctg aaccttgcct ggatgtatca
     1561 ggaaggaaaa ggagtatcac aggacgatgt aaaggctgcc aagtggtatc tgaaagcggc
     1621 tgagcagggt aatgcacagg ctcaattcaa tctggccctc atgtacagca agggagaagg
     1681 tgttgcactg gatcatattg aagcggcaaa atggtttttg gaatcggcgg aacagggtat
     1741 cgccaatgcg caatataatc tgggcatcat gtaccaggac gggttaggtg tccaaaaaga
     1801 ttctaccgaa gctgtcaaat ggttcaataa ggcagccaag cagggacaca cggatgccca
     1861 ggctaaactc gatgccatgc gcaaagccct gcaggacgag cagcgggaag cagcccgggc
     1921 ggtcaaggaa tcccctcgca tccttccatc aaacgccgcc gatgcttcat ccataccgga
     1981 cgagtttccc gacgagaaga aagaagccct gtcacaggac cacgccaaac aaatccttgt
     2041 aagtcaatat ctgaaggcgg ctgaacaggg ccttgccgat gcacaatatc atctgggcgt
     2101 tatatacgaa gaaggttccg gcgttccccg ggataatgaa aaagctataa aatggtatcg
     2161 cgccgcggcc gaacaggggc atccagaggc ccaaaataaa ctggaggctc tgtctccggc
     2221 cggtcacgaa accatgccag gcgatacaca gccccccgtt gacggaaacg aaaccactgc
     2281 atatacaaaa ccggatgaaa gcgcgaacgc gcttatagtt gtaactgatg agcttgctga
     2341 acaaaatcat gatattgagc aggaggttcc ggaaacaaca gcgacggaaa gcaatccctc
     2401 gccggagatt tctgatgacg aaaaagggta tcgcgaagcg gccgaacagg gcgatgccga
     2461 tattcaaaac cggctgggct taatgtataa aaacggcgac ggcgttatgc agaaccacgt
     2521 tgaagcggca aaatggtttt taaaggcctc ggaacaaggc ttcgccgatg cgcagcttaa
     2581 cctcggcaac atgtaccagg aggggtcagg tgtcccgcaa aacgatgctg aagcggcaaa
     2641 gtggtatcgc gaagcggccg aaaaagggca tgccggtgca cagtacaatc tgggattaaa
     2701 atattttttc ggggaaggtg ttccccggga ctttgctgaa gcaatcaaat ggtaccaaaa
     2761 agcagcgctt caggacaatg tcgatgcaca attcaccctg ggcggcatat acgaagaggg
     2821 tgttgttatt ccggaagatc ctgctgaggc tgaacggtgg tatcgccggg cggcggaaaa
     2881 gggccatgag cgggctcggt atcgtctgga attgatgcac aggcacgtct gatcgattca
     2941 gcacaagcca accgcaacgg attttaagaa aaaatcctgt cttgatccac cgcgccaaaa
     3001 caccgcttga ttatttgtca gacgaaccgg gccgttttca gaccaatacc aaacatccgc
     3061 ctgccgttgc gcgtttttta ttcacccggc aagcactgaa agtataaaga tttttttacg
     3121 ctctataaac ttcattttca tcctggaatc cccaacgaat aattcattga cacggcaggc
     3181 tgattatcat atgaactctc acatcaataa caaggacaat gggttacggt gatttttctt
     3241 ttaaataatt ttcaaaaaat aactgccttc tcgggttcta tgttgaaaat ctcgcgacac
     3301 atccatgaaa aaagatttcc tcagattctc acactttttt ttacgctgat ccttgtatgt
     3361 ctgacgatgg tctggtcggc cgatccggct tattccaacg atacacagga caaactttat
     3421 tctctcaacc tctactatgg cgaaatatcg aaaaatcatg ccgatgattt cattttggac
     3481 accggaacac tccggctgac cgattgccgc ctggcagccg tcacgctggc acgccgtctc
     3541 tatgcataca aagatctcgc caatctggaa gttgagggac agattgtaaa gcatttctac
     3601 gacatccagg accactggga atttaacgcc attgtaacgg ctcgctggaa tgcctttttt
     3661 tgggacaagt atgtggatac aagcatcgcg ttcggcctgg gcccctctta tgcaacagag
     3721 aaaccggaaa tggaagcgat tcttgaccgt gagacatccc aatggctggc gtattggatg
     3781 gtcgaactgg aaatgggtct gcccgcccat cccggcatct ccttcattac gcgccttcat
     3841 caccgctcgg atgcctttcg cattgtatcc ccgggcggcg cctccacagc acccgttgtg
     3901 ggattgaaaa tccggttctg acaattcgct gttaaaatcc gcatcgatgt cttcccgacg
     3961 gggaagcggc cttgtttaca aaaaatatca acaacaaacc ttgctatgtt ttgatgagtt
     4021 atgataagcc atttccagcg gcaaacatcc gcaatgatga gatttctttc gagaagatat
     4081 aagaaaaggg gcccgttgtt tttttttgcg gcccggtgta aaaaacaatt aagataaaag
     4141 ttccgtaaaa acattaccat gaacacaaaa gcaaatcgtg ccataggcgt tttcgattcc
     4201 ggcatcggcg gtctgacggt cgtccgggcc ctgatggaac gtctgccttt tgaaaacatc
     4261 atctatttcg gcgacaccgc tcgcgtgccg tatggtgtga agtcggtgga aaccatcaac
     4321 cgctacgcgg cacagattac cgaatttctg atccaaaaag atgtcaaaat gctgattgtc
     4381 gcctgcaaca ccatggccgc agtggctcat caaacgatca ttgatttatc acaggtgcct
     4441 gtcgcgttcg cttatcaaaa acttgtggaa caggtacccg tcctcgaagt cattgacgcc
     4501 agcgcccgca tggccatcca gcataccaaa accaagtcca tcggcgtcat cggcacacct
     4561 gcaaccgtca acagcaatgc ctacgccagg gcaatccatc ttctggataa agaggcaaaa
     4621 gttttctctc atgcctgtcc tttgttcgtt cccctggtgg aggaaggatg gtttgatcat
     4681 ccggcgacaa aactcattgc tcaggaatat ctcaaacagg ttatcgccga acaggtggac
     4741 acgctggttc tcggctgcac gcattacccg cttttaaaac ccctgttaca ggacattatg
     4801 ggaaaatccg taacactgat cgattcggca gaagccatgg ccggtatcgc cgccgatctg
     4861 atcgatcaac aagcgatcgg caatcagaac catgcggtac cggaatacac gttttatgtc
     4921 agcgatttgc cctatcgttt tcaaaccatc ggcgaacgtt ttctgggaag aacgctggga
     4981 aagattgaac ttgtcagttt ttaggaaaat ttcatgaaaa ttcttcttgc cggtcataat
     5041 ctggactatg acatcatccg gacgctgaaa gacaaaagcg gaacaaaaca ggatttgaca
     5101 cctgaaacca tctcggcggc ttacgcacgc atcagccgca atccgaaacc cgttaacgag
     5161 ctgcgtgcca tcgcccgcga ggaagtggat aaggctcgcc aatccaaccg taaaatcgtt
     5221 tttgacatgg ggcacaactc cgtggccgag catgcggttt ttaatatcga tgtgatcggc
     5281 gtctcccggt tcctggttga agaaatcgaa aaattccgtc tgtgttcctt cacggaaaaa
     5341 tcacagcgct atgttctttt cgataaggat tttgtcgtcc cggaagaaat ccggcaggct
     5401 gatcttgccg gtttgttcat ctctaccgtt gaagcgcaaa atgattttta ccatgagatg
     5461 tatgagaact tgaggccgta cattttcgat aaacataaaa ctctggcgtc cgatccggcg
     5521 aaccgatcca tgctggaagg ctgggccaag gaagacgccc gctacgcgat ttccatggct
     5581 accgaaaccc aactgggcat gaccgtcaac gcccgcaacc tggaactcat gctcagacgg
     5641 ctggcggcat tgcccctggc cgaagcgcag gaatacagcg caaagctgta tgccgccaca
     5701 aaggaaatcg ccccttctct gatccgttac acgaaggcga cagactacga taaattcaca
     5761 agaagaaatc tgaatcggtt ttccgacgca ttaataaaaa aatcgcaatc gaaggtcaaa
     5821 aacgcagccg atgtgcaatt gatgtctgca tcggctgctg ccgataaccg ggcggctgcc
     5881 gcattgctgt ttgcatcatc ggatgccaat tatgccgaat gcctgaagac ggccgacaag
     5941 ctgaccgctc agaaaaaaag atccctgttt aaaactgctt ttgccaacct gcagatacac
     6001 gatgccgtat taagggagct ggaaactgtg gatctgcagt ttgaactgat cgccagttcc
     6061 agttgctttg cccagcttaa acgacaccga atgtccacta tcattgcaca ggaatacaat
     6121 ccgcatctgg gtgtcaccgt gccgccttcc attaaagcca tcggccggca aaaagatttt
     6181 ctggacctga tgcaccgtac gcagagcaca tatgaaaaaa tcagagagaa agcaccactg
     6241 gctgccgttt atgtgctgac caatgcccac cgtaaaaggg ttttgatgaa actgaacgcc
     6301 cgggaaatgt atcatcttgc acgactgcgt gccgatgccc atgcccagtg ggacattcac
     6361 aatcttacgg gaaaaatgct caaacaggcg aaaaaagtga tgcctctgac gctgatgatg
     6421 gcctgcggga aggatcattt cccgtcgctc ttgaacaaaa cgtatagctg cacgtaattg
     6481 tccgtattta tcatccatcc atgaaaatca acgccaataa aaatcatctc tatgtgctgc
     6541 tggttacaac gctgagttct tttctgacac cgtttatggg atccgccatc aacgttgccc
     6601 tccccgccat cgccgaagaa ttgtccatga ccgctctgac gctcagttgg gtggccactt
     6661 cttttattct ggcggcatcc atatcgctca tccctctggg acgtctggcc gatatctacg
     6721 gccgccgaag aatttttatc tacggggctt tgatttttac cgtggcttcc gccttttgca
     6781 tctggtcgcc cacgcaggct tttctcattg ccgtccgagc tttgcagggc atcggcgggg
     6841 ccatgatttt cagcaccgga acggccatgc tgatttcagc ttatccgcca agcgatcgcg
     6901 gcaagattct gggcatcaac atcaccgccg tttatgttgg tttgactgtc gggcctttta
     6961 tcggcgggct gctgacgcaa tatctcggat ggcggtacat ttttctgttt accgtcttcc
     7021 tgggcttaac catcattttc atcacgctgg caaaaattga accggaaaca tccgcccgcg
     7081 aagacgaggg cttcgattta accggcgcgc tgatctacgc cattgctctt ttttccatta
     7141 tgtacgggtt ttcccatctg ccggcattta gcgcggggtg tctcattgcg atcggtatga
     7201 tctttctggg cttattcatt tttcagcagc tcaaaagccc cttccccttg ctggacatac
     7261 gcctgtttct cgataacaag gtttttgctt tctccaatct ggccgccctg atcaattaca
     7321 gcgccacgtt tgttattaat ttcctgctga gtctttatct gcaaaacatc aaagttctga
     7381 ccccttccga agcaggctcc attctgatcg ccgccccgat catgcaggcg cttatttcgc
     7441 cttttgccgg acgtctttcc gaccgttttg aaccgcaaat catcgcgtcc atcgggatgt
     7501 ccatcacggt cgcgggtctt gtccttttaa ttttcctcaa cggttccacg ggcattcatt
     7561 atgttctgtt ttgtcttgtt ttactgggca tcgggttcgc gctcttttcg tcgcccaatg
     7621 tcaatgccac catgagttct gtggaaaata aattttacgg tatcgcatcg gccacactgg
     7681 gtacgatgag gcttaccggt caaatgttca gcatgggcat tacgatgctg atttttgccg
     7741 ttattctggg caaccatccg atttccgccg ccaatcatca tctcttttta aaaagcacca
     7801 aacttatttt ttccatcttg agtattcttt gctgcgcggg gatctttgct tcactggcca
     7861 gagggaaaat gcatgaaggc aatcactctc tctagttgta ttatcggact ggtcgccggc
     7921 acgtttggcg gtctgatcgg catcggcggc ggtatcatta tggtgccgct gatggtcgag
     7981 atattaaagc tgccccagca caaggctcac ggcacaagtc ttgtggccct ggttttcaca
     8041 ggcatcagcg gatcaataac ctatgcactg aacggatcga tagacatcaa agcggcggca
     8101 ctgctggcgc ttacggcaat catgacggca tccctgggcg ctcgttacgc cggttccctt
     8161 acggaacaaa aattaaaaaa agctttcggg gcctttctgc ttttctgtgc agcactctta
     8221 tttttaaaac tatacttgcc gaatgtcgtc gtaagcgatg atgcaaaacc ggttatttat
     8281 ctgacaacag gagcgttgac cggatttctt tccggcatga tgggcgtcgg cggcggcacg
     8341 attatgattc ctgtgatggt aatacttgcc ggctttaccc agcatgtggc acagggaaca
     8401 tcgctgctgg tcatgattcc ctcgggcgct gtcggcgcac tcacacactg gaaaatgggc
     8461 aatactgaac aaggaatcct ctgggggctt attcccggga ttcttttagg cacatacatc
     8521 ggaggaactg tggctaatat catcccgaat gataccctgc gcctgatttt cgccggcatg
     8581 ctgatttttc taggcattcg ttattttggg atgtcttccc cccgaagaat aggcaagggt
     8641 tgattatgaa aaaaacagat ggctatcgta aattttcatt tttcaccgac gttcgcggaa
     8701 tgaaaaatgc gatcggtgaa atgcgtggat ggggccatcc tcatcagcca atatttaatg
     8761 ccggttatca gcgcttgcct gatgatcccg tattcaatga agattggttt atcagtacca
     8821 tcaaagaaaa gatagccact catccggaaa ataccatgga atatcccttt tacggagaga
     8881 aaatcttcac ggagccgctg ataggattta ttcagggtga cgatccgata tttcaacagt
     8941 ataaaacgat tatcggaccg catcacttca cgccggaaga aattatgcag tggcaggcca
     9001 ggcaacatgg cgttcccgct ccccgggcag aggatttaag cgtcgtatct ttcatcctgc
     9061 cgctgacgaa aattacaaag aacgataatg cggccatgaa agaatggtcc gcggaacgtt
     9121 gggcgcagac gcgcctctta ggtgaaatat tcagccagat attcgtgcgt gaaatagtta
     9181 cctgtcttat gaaaaggggc attttggcca tagcgccgga cgtcacgccc atgtttaata
     9241 aaaaacgtta tcccaaagta ggctgggctt ctccctggtc gcaccggcat atggcgtatg
     9301 ctgccggtct gggaaccttc gggatgcacg attttttcat cactgaaaaa ggctgcgctc
     9361 acagaacggg cagttttgtc gtcaatgtaa aacttacatc aaaccgtgag cgacctgatg
     9421 atattcatgc tcattgtctg caatatcaag gcaaatcctg catgcaatgc gcgaaacgct
     9481 gtccggtgaa tgcgataacc gcggaaaatg gccataataa ggaggtttgc tatcaaaggg
     9541 tggccaaatc tctgatacac tgcaataaaa attatcacat ttttatctat gggtgcggtc
     9601 tttgcgcaac cggagtcccc tgtgaatccg aaatcccgaa aatcctgcaa aaaaattcaa
     9661 aatgaaaata acaaaaaaac cggggtttat tccggctttt ttgttatttg tttttaaagt
     9721 ttcagctttt taggagctgt tcaaaaaagc ttagacacaa ggcacgtgcc cggcattcgc
     9781 cgggtttccc tttattttaa aatgggtgct acgcgagcat ctttgtctgg acgcctttca
     9841 aaatatcttc cgtctccttc atcatggatt gcaccatatc cgcgactttg ggcatgtcgt
     9901 tgatgcgttg cgctgcttcg cctgctgcca gaagcgcctt ctctttgttg ccgtcgtaag
     9961 cagcctgcat cgactccatt tcaaactcaa ttaatttgaa gtcgatcgac agcatatcat
    10021 ccggcgttcc cagataacat ccgggcgatt tctgcagtgt gttttgggcg tgctcttcac
    10081 tgcgcggatt tttaagccat cgcgccggcc cgacaaagcc gcgggcaacc agagtgcctc
    10141 tgtcacctgc ctcgaccaca ccttgtcgcc agatcggggg aaaatcgctt tcttgtgttg
    10201 caagaaaacg cgtgcccatc tggacgccgt cggcgcccat caccagggcc gcggccagcg
    10261 tcttgccgtc gcagaaaccg ccggctccgc ataccaaagt tttctcatcg gaaaccgctt
    10321 ctacaacagc aggcagcaga atcatggaat gcaccggttc ccaggatgta tgaaatcctc
    10381 cctcgtgtcc ggacgccacg atgacatcaa cccctgcttt tttgcagcga agagcccctt
    10441 ttactgaagg caccacatgc atccaggtaa aaccggcgcc tctgattttt tctttccagg
    10501 gcaccggatc gcccgcagac gtgaaaataa ccttgaaatg tttttccatg cccggattgg
    10561 ctgcccgcac ctgaatggcc gcattgatga taagctccgc ctgaaaaagc aattcggcgg
    10621 agaccatgac gttgataccg aataccccgt ttgtgtcttt agtcaaccgg taggctcttt
    10681 tgagcaattt ctcaattgcg gtgggaatgt cgtcatccgg gttggcctcg gcgctcttaa
    10741 tccagtcatt gtaaataaag ggcagaaaat ccttattgca gagtccgctc gtggaaaaca
    10801 agcccagcac tccggcgttg gcggcggcta cgccgagatt gttgttactg aacggtccca
    10861 tgcccgcctg aatgatggga tatttgattc cggtcatgtc gcaaagtctt gatttgatca
    10921 tataagacat ctcctataaa attattgtat cttatacaca tcactctctg atgcgctttt
    10981 tgagttctcg aatcatatga ttgagcacgt cgcttgcatc accgatgatg gcgacatcgg
    11041 ccattttcag catggttgcc tgcggatttt tattgatcgc aatgatgaaa tgtgcctcgc
    11101 ccaagcctgc ggtgtgctgg atcgcaccgc tgataccaaa ggaaaacagg agtttcggct
    11161 tgatatgctt tccggcctga cccaccagtg cgtcgtggtc cgcccatccc gcgtaaacca
    11221 ccggacgggt ggcgccgaca tcgccgccta aaagtctggc cagttcctga agttttttaa
    11281 atttttcctt attgcccatg ccgcggccgc cgcaaataat aaccgacgcg ttttcgatgg
    11341 tatgctcttt ttcaacggaa cgttcatatt cgataagacg aacgcggggt ttggggagat
    11401 ttttcatcaa aggaaggcgt tcaaccaacg ctttccgctg gttatcgtga ggaatttcct
    11461 taaaggtgcc cggacgaacc gtagccatct gcggacgatg gcgctccgtg acgatttccg
    11521 ccagcatatt gcccccgaaa gaaggcgcga tctgcagtag cagtccgtca tcggcgatat
    11581 ccagcccgac gcagtcagca gataatcccg ttttcagtcg tttggccagc cggggcgcaa
    11641 attcacgtcc gaaatccgtt gcgccgatca gaataatttc cggatctctt tctccggcca
    11701 atcgttcaat gatggccaga tatttttcgg tgctgtaact tttcagcgcc ggatcatcaa
    11761 tcgccaaaat acggtccgcg ccatgcgcag tatattccat aatccattcg tctatttcat
    11821 gaccgaaaat caatgcgcaa acttccgcgt tgatgacctt ggccaacgca accgcccggg
    11881 cgatcaattg cagggtgacc cggttttgat aataattgcg gtaatcgccg aatacccaga
    11941 tacttttttt actatttttt ttcatcggac ttcttttctt caatggattt ccctatgaca
    12001 gtgccgattt ttctttgata cttgtccaaa aactccgtca ccaccgcaga cgccgctccc
    12061 tgcagaagca cgttcccttt ctgcgtcttc ctcagaaaca ccttgcggac tttggtgcgg
    12121 gagccttttt tttcaatgga tgcagccgtc aagcccagag ctttcgcatc caggatgaca
    12181 acatcattct tgtcgaaggc gctttccaga cccgccaacc ccgtataacg cggtttgtaa
    12241 ttttccatcg agatggtcac cagcgccgga aattccattt ccagtgtttc gcggaaatta
    12301 tccaccagac gccggacgcg taatgttttt ccggaaattt ccagtttttc cacatacgcc
    12361 gccgcgggta aatcaagctc ctcggccagt tgcggtccga cctgggccgt ttcgctatcg
    12421 gccgtgtagg ctccgcaaag gatcaaatca aatttcggac atttcttttt gatcgcctct
    12481 tttaaaataa gggacgtggc aaacgtatcc gatccggcca acagcctgtc gcaaaccagc
    12541 acggcttgat cggcgcccaa cgccagcgct tcccgaagca cttcctcggc ggccggcggt
    12601 cccatcgtga ttacggtgat attggcggga tggtgttcct tgatttgcag agccgcttcc
    12661 agggcgtgtt tacaggccgg attcatcatg ccggcggcaa ggtcgcgccg cagcgttccc
    12721 gtcttttcat cccacggcag ttcggttacc atgggaacct gttttaaaca aacgactaaa
    12781 ttgagcatgt ttataatatc aactgagaca gtatggatcg cgcgataacc atgcgctgaa
    12841 tttcgctggt accttcgtaa atttccgtaa ccttcgcatc gcggaaatac ctttcgacat
    12901 cgtattcctt gctgtatccg taaccgccgt gaatctgcac agccagagac gtcaccctgg
    12961 atgcgaccgt actgcaatac aactttgcca tggacgcttc acagccaaaa ggcattccct
    13021 catccttcaa cgagcaggcg cgatacaaaa gcaggcgcgc agcggcgatt tcggtggcca
    13081 tatcggcaat ataattctgg atggtttgca gcgaagcgat gggtttttta aactgctgtc
    13141 tttctttggc atatttaacc gccgcttcaa aagcgccctg agcgatcccc agcgcctgcg
    13201 cggcaacgcc gatgcggccg ttatccagcg tagccaaacc tattttcatg ccgtcgccga
    13261 tcctccccag cacattgttc tccggcacac agcaatcttc aagaaaaagc gacgaaacag
    13321 gattggcgcg cataccgcag agatcttcca gttcgccgcg taaaaatccg ggatatttgc
    13381 tttcgacgat aaaaacgctg ctttgcggct tgccctgttc agagccggtc agcgcaaaaa
    13441 tcaaagccag atcgcagacg ccgccgttgg tcacaaatat tttcgtcccg ttcaaaacgt
    13501 acgcatcatt tcttttgacc gccgtcgttt ccacgcttgc ggaatcggac ccggcgttgg
    13561 cttccgtcag actgaatgcg ccgatcaact cgccttcggc cagtttgggc agatagtgtt
    13621 tcttctgctc tgcgttcgca aaccgtaaaa agggatatac ggcaacacca ttatgcaccg
    13681 tcacgcacaa accgatcgcg gcgcaaacac gggagatttc ttccacaaca atagcggcgc
    13741 tgacggaatc cagggcggct ccgccgaatt cccgcggcac ctgcaggccg aaataattca
    13801 acggcctcat tttctcgatg atttcacggg gaaaagttgc agcgcggtct attgcggacg
    13861 caacaggcgc aagttccgtc tctgcaaacg tccgcacgga atcgcgaatc agtttatgct
    13921 tggcgcaggg attcagctgc aatgttctct cctggaagaa aatgctttgc acaaataact
    13981 taaaataacc gtaaaatcaa gaaaacctta ttccgaaggt ctttgcccgt ttccgggaac
    14041 cggtactaat cctcagggtt atcaaacgca tgatatgtca ttgacagccg ctacggttga
    14101 ctctatactt ctatgctatg gtacaccgcg aggccaaaaa taaatcaagc cggaagacca
    14161 aagcagtcca tcatgacccg gacgctcttc cctcgttaat ggccaatcct caggaaaaac
    14221 tcgctgtttc gctcccttta cggcttatca tccgactggc tttaggattt gtcgatcttt
    14281 ttcatatttg gcgttttccc cgcaagaccg acggttgctt cgaacacatg accctcatcg
    14341 ataagttgca ctggctttac aaatcggcaa acccgatccg tttagccgaa aaaaaatctt
    14401 ctcttgagga ctttttcgcc ggacaggcgg aaaccagcct tacgctgccg ccggattttg
    14461 aacaaaccac tcaaatacaa ctggctgccg tgggcgatct gatgcagaac gatctgctga
    14521 cgcattcggc ggattcgctc tatgaaggca ttgccgccga attatttact gcggatatct
    14581 cctttgccaa tctggaagct cccttgacca ctcagccgca acgcaattta ttattcgtcc
    14641 gcagtgagac cccgtcaacc tacatttcgg ccgggcaatt tcaggtgctc aaaggataca
    14701 aagatcagaa attcaccgtc ttctcaactg ccggcaacca tgcctgggac tatggcgagg
    14761 agggcgtgct cagcaccctc gacgctctgg caaaagaagg gattgtcgcc gttggaacga
    14821 atcgtgaaaa agcggatcga cagaaggcgg cgatcattga tgtccaaggc atcagaatag
    14881 gctttgtcgg attaaccttc ggcctcaacg gcaagaaaat cgatccggaa caggaatatc
    14941 atgtcaattt ggttcaactg aatgctttat ctgcggaggt cgatctgtct gtgcccatcc
    15001 ggcaaatcaa ctactgccgg gaacagtact gcgattttat cgtcgggtcc ctccactggg
    15061 gtcttgaatt tgaatttttt cccgcccgac gccagttggc cgttgctcat caactggccg
    15121 aagcggggct cgatctgatc atctctcacc atccgcatgt tgtccaacct gttgagtttt
    15181 accggacccg gcgcgatccg caacggacgg tggtcattac ctattccctc ggtaacatgg
    15241 tcagtccttt ccacgacgac gaccttaacc tgagttttct tctgcaggtg accctcgccg
    15301 acgggaaatt gaaaaatcaa aagcgaacct atattgccgc ctgccgcatt attcccgtct
    15361 gtcagagagc attgatggcc ggcggaaagc cgacggtgcg gctggaaaag gtaacccgtt
    15421 tggccgcatt ccggctgccg tttgacctca agaatcttgc tccataaaaa ttccgcatga
    15481 ggtcaagcga tgacattttg tcgctgccga ttgtccggag ccgtgggatt tgctatgtca
    15541 gcggcttcgc cgcttgcaat gacacgctat ccgttcacgg tacaacaacc tttcttcatc
    15601 aaccccttaa tgcactcttc tctgttgatt taaaacgttt aatggttatt atatacccga
    15661 tgagcaagga aaagattcca acaacaccgg cggttttagc cctcaaagca caacaggctg
    15721 atttcactct gcatacgtac ccctatgagg aaaggggagg aacaaaagca tccgcccgga
    15781 aactgggcgt tgacgaacac atggtcatta aaacgctggt aatggaggat gaaacgggta
    15841 aaccgctgat cattctgatg catggcgatc gggaagtgtc caccaagtcc ctggccaggc
    15901 agatcggagc caaatccgtc accccatgca agccggccgt cgcggaaaaa cataccggtt
    15961 ataaagtcgg cgggacatcg ccgttcggga ccagaaaagt gttgcccgtg tacatggaac
    16021 agaccatcgc cgatttgccg gatattttaa tcaacgcggg cagccgcggt ctgctggcaa
    16081 ggatgccttc ggccgaactg gcgcgtctgc ttaagccggt catggtcggc gttgccatat
    16141 gatttgcctt tttcgggcaa aaaagagata atgcctttga ggataaatgc tatgcctgat
    16201 gacgtgatta tccgctgtct taactgcgga accaaaaaca gaataccgaa acaaaaactg
    16261 cagggacgat cgatctgcgg caagtgcggc gcttcgctgg atgaactcat catccgctgc
    16321 ctgaaatgca gcacgaagaa cagaataccc gaggaccgat taaatcaacg gcctttatgc
    16381 ggcaaatgcc atgaaccgtt aatcattcct cagcaaaaag ttttgccgtt cgatgtcaca
    16441 gaccagacct ttgcccgcga agtgttgacg tccgactctt cggttctggt cgattgctgg
    16501 gcgccctggt gcggcccttg ccggaccctg tccccgatta ttgacgaact ggcctccgac
    16561 tatgccaacg gcgtgaaagt cgtcaagctc aatgttgacg aaaatccgct gaccgcctcg
    16621 caatacggca tccgcagcat tccgaccatg ctttttttca aggaaggcaa aatggtgcat
    16681 cgtgtgaccg gcgcactgcc caaagaagaa atcgagcagc atctcttaac gatcatcaaa
    16741 accaactgac cctgtggagt attcatggcc gacaacaaat cctctatcgt ctaccaatgc
    16801 aaaatttttg atgtccggga agaggaggtc accttcccaa acggcaaaac ggccagacaa
    16861 agctggatca accatcaccc cacggtggcc attgttgctg tcaatgaaaa aaatgagctg
    16921 ctgctcatca aacagtatcg caacgccgtg aaacaggacc ttttggaaat accggccgga
    16981 tcgctcgatg ggatcgaaga atctcctgcg gtttgcgcgc aacgggagct ggccgaagaa
    17041 accggtttca aagccaaatc gctgatcaag ctctacgaag gctatctgct gccgggttac
    17101 tgcaacgaat acatgtattt ttttctggcc cttgatcttt tcagcgcacc gcttaatccc
    17161 gacgacgacg agttcattga aaccataccc gccacgtttt cccaagcaca caagctcatc
    17221 gacagaggcg tcatcatcga cgccaaaacc gttctgggca ttttgctggc tgaaaaactt
    17281 ttagggcggc aaaacgcctg atggattctt gtttcccgag aaagcttctt gccagagaca
    17341 aaaagttttg ttaggctttt acacgattcc gatatccgtt aatccatgag gaggctgcgc
    17401 catgatctac aacgaagaat ttgaaaccat gccccgcgaa gtcatcaaag ccctgcaggt
    17461 taaaagactg cagcaggttc tggagcgtgt ctatcacacc gtcggctttt acaaaaaatc
    17521 ctttgatgcc gccaaagtga aacccgatga cattcgctgc atggccgaca tgaaaaagct
    17581 tccgttcacc accagaaaag atttgcgcga caactatccc ttcggtttgt tcgccgtgcc
    17641 catgagcagc gtggtccgtc tgcacgcatc gtccggaacc agccgccgct ctgccgtttt
    17701 cggctacacc aaacgcgaca tcgacacctg gtcggatctg atcgcgcgct cgctggtcgc
    17761 cgctggcatc accaaaaacg acatcatcca taacgctttc ggctacggcc tcttcactgg
    17821 cggactggga ctgcattacg gcgcggaaaa aatcggcgcc agcgtcattc ccatctccgg
    17881 cggagacgtc aaacgacagt tgatgattct gcaggatttc ggtcccaccg tgctatgcgc
    17941 gacgccctcc tatgccctgc atctggccga agaaggcaac gccatgggaa ttgacatcaa
    18001 agcgctgaaa ctgcgcgtgg gcattttcgg agccgaaccc tggaccgacg ccatccgcga
    18061 tgacatagaa aaagcattcg gcattaccgc gctggatttg ttcggactgt cggaagtgat
    18121 cggaccgggc atggcgatgg aatgcctgga aggccgccac ggcatgcaca ttttcgaaga
    18181 ccacttcatc gttgaaacca ttgatccgca aacaggcgat gtgcttcccg aaggggccga
    18241 gggcgaactg gttttcacca cgctgaccaa agaagccaat ccgctggtcc gttaccgcac
    18301 cggcgacata tcgcgtctga ttaccgaacc gtgccgctgc ggccggaccc acatcaaaat
    18361 ggaacgagtc ctgaagcgta gcgacgacat gctgattatc cgcggcgtca atgttttccc
    18421 gtcgcagatc gaagccattc tggtggagat cgaaggtctc gagcccaatt atcaggtcat
    18481 catcgataaa gcaggggcgc tggatgtcct ggatttgcag gtcgaagtca acgacaaaat
    18541 cttcagcgac tcgggcagca tcaaacaatt gcagaaaatc gaacaaagaa tcgtcaagga
    18601 catgagagat tatctgggtg tttccgcacg cgtcaagctt gttcagccca acacgttgaa
    18661 gaaaaccgac gccaaaatta tcgacaaacg ccgtatttga ttaaccacaa aagggggttg
    18721 ccatgaaagt agaacaaata tccgtatttc tcgaaaacaa acccggcacg ctggaagacg
    18781 caacgcgcgt tctgaaagaa gccaatatca atatccgcac attgtccgta gcggaaaccg
    18841 tcgatttcgg cattctgcgc ctgatcgtca atgacgtcga aaaagccaac aaagtcctca
    18901 aagacaacgg tttccgtgta agcaagaccc ccgtcgtcgc cgtggaagtc cccgacacac
    18961 cgggcggact gcacagcatc atggaagtgg tgtccaaaga aggcatcaat gtggaatacc
    19021 tttacgcctt tgtggaaaaa agcggccaga acgccgtcat catcttccgc ttcgacaacc
    19081 ccgaaaaagc catcgatgtt ctgacgaaaa agaattttac ggtgataccg ggaagcaagc
    19141 tttacgagtt ttaatgagac tcgaatctta caagcgcagc acagtaagat tcgttagtcg
    19201 aactacccca actgcgcagc agttgtgggt aatgacatca tctagagata taatgaactg
    19261 aaacttgatg gttgaattat cctaggagcg tagcgacgca ggataatgag tccccaaatt
    19321 tctgaaacac tgtgcacagt aagattcgtt agttgaacga ccccaactgc gaagcagttg
    19381 cggatattga catcctttaa aaaaacggtg gatcaataat tttatccacc gttttcacta
    19441 tccaccagcc accatcaact attaactatt cacattttct tctgccacgg cttggtcttc
    19501 caccgctggt ggaaccagaa atattgatcg gggtattcgc ggataacctc ttcaatgatc
    19561 tttgtgaagc gttgcgtatt caccaccaag tcgttttcgt aattatccgt acaaaccgct
    19621 tcaatcgtcg gtttgatgat aaatttatat ttgcctgatt tttgtcgggc cataaaagcg
    19681 ggaagaacgg gagcgccgga acgcatggcc atcaccgcca ggccgactcc cgtacaggcg
    19741 gtgcgaccga agaaatcgac aaacacgcct tcataatggg cgacgttctg atcgctgaga
    19801 atgccgatca ggttattttc gcgaagcaac tccataattc tttttcccga gccccctttg
    19861 ggaatcatat cgttgccctg catggtccgc acatattcca cgatattatc gatgacgtga
    19921 ttatccagcg gccggtacac aatgtacatc ggtttgagat acatcggacc ggcaatcgtc
    19981 atcaattccc agttgccgaa atgcgccaca atcgagagca ttccttttcc tttggcaata
    20041 cccgcctgaa aattttccac accttcaaca tcaacccatt tatggatatt gtctttggtc
    20101 atgtaaggca aatcgaaaaa ctcagcgaca acgatggcaa aatggcggta aacgcctttg
    20161 gcgattttga taatttcctt cgtgtccttt tcggggaaag aacgcatcag attatgcagc
    20221 gtaattaaac ggctttttgt gccgatgtga taaaaaagaa aaaaaatcga taaaaacatt
    20281 cccttgcgca cacatcgggg aatgccgtga aaaacagaaa gcaatatgtg ggtggttctg
    20341 gttttttcaa ctggcatagt tatttatatt tacagcggct tgccgcattc cttgcatttg
    20401 ccgtccggac cgatcacacc gatgcatgac ccgtcggagc acaatactct tttatcccaa
    20461 tcgtcatccg cttgatcatc gggggaaact tgtttttccg gacttacttc atgaacgatt
    20521 tcatttattt tgccttccgg caattttcct tcgtaaggtt tgccgcattc cttgcacttg
    20581 ccgtccggac cgatagtgcc gatacaggac tcatcgctgc acaatactcg cttttcccaa
    20641 tcttcatttt catcaaaaat gatttccgtc acggcgttct cccggaattt ttttcactgc
    20701 gaaatctttg caccatgctt aacttgtcat ttcgaacgta tgtgagaaat cttaaagatc
    20761 cctcgctacg ctcgggacat ggatttctcg tcgctttgct cctcgaaatg acacgagcgg
    20821 gtgttatgca aaacgtttaa ctcttcttac atcagctttg caatttttac cacaaaacag
    20881 tgcatctttg ataacttcgt aaaaagttca gaggcaaggc gtgcgaagcc cgattaaaat
    20941 aacacgcatt ttcgtcaggc atcattgcca agtcgcattc agaaggatac cgcggcggca
    21001 gtgaaactcg aattgccgtt acgaagtaaa cggtaattcc ttagttgaac tgtcctgcct
    21061 cagctgggcg aggaggaggc tccgcaggcg tatattataa tacgttgagg aagccgacga
    21121 cgctgccaac aaaggtagcc gcatgaaggc cgtctttacc ttaagccttg ataacttcgt
    21181 aaaatgtcca gaggcaaggc gtgcgaagcc cgaggagtga ggcgtacttt tggcgtacgc
    21241 cgcaacaacg acggctgaag cacaacgcag tatctgggcg ttttacgaag ttatcaccct
    21301 tttatgcacg aaagggattt taaacgtgcc acttttctgg caattcccgc ctcatgcatc
    21361 acctgcacaa catcctgcac atttttatac gcttcgggca tttcttcact gagcgttccc
    21421 ttggaaccgg ccatcaccaa aacacccttt tccgaaagtt cacgggcaat ggaacgcccg
    21481 cggctgtttt tgatcgcctg cgaccggctc atcacccgtc ccgctccgtg acaggcgctg
    21541 ccgaaggttt cggacatcgc tttttgctga cccaccagta cataagaccc cgttcccata
    21601 tcgccgggaa tcagcaccgg ctggccgact tttctgtagc gcacaggcac cgcctcatgc
    21661 cctgcaggaa aagcgcgggt agcgcctttg cggtgaacgc aaagtttttt tatcacacca
    21721 ccgatattaa acgattctat cttggcaatg ttgtgacaga cgtcataaac caatcgcatg
    21781 ttcacgtcgc gcggcgacat ccgcagcgtt ttctcaaata cttccctggt tttgtgcatc
    21841 aggatttgcc ggttggccca ggcgtaattg gcgccgcagg ccatggccgc cagatagttt
    21901 ttgcctcttc ccgaagaaag ataagcgcag gccaactgcc tgtccggaag cgcaatcccc
    21961 gtttcgtttg catgtttcac catcaacgcc aggtaatcat cgcaaatctg ataccccagg
    22021 ccgcgcgaac cggtatgaat catgacggca atctgtccct tgtgcaaacc gaaggctttg
    22081 gccgcttctt catcgaagat ttcctgcaca acctcaattt ccagaaaatg attgccggac
    22141 cctagcgttc ccaactgctg cttgccgcgc tccagagcac gggctgaaac cagttcggga
    22201 tcagctccag atatcgcgcc gccgtcttcc gtagtttcca aatcttcgtt taaaccgaag
    22261 ccggctttga tcgcccaggc ggctccctct tccagcactt tcttctgatc cttgcccgat
    22321 aacttcacgg aaccggtcga gcccacaccc gaaggaatgc gctgataaag ggcggttgtt
    22381 aaatcactga gccggttttt gatatcccca agcgttaaat ttgtggtcat cagacggcag
    22441 ccgcaattga tatcgtagcc gacaccgccc ggagaaataa taccgccatc cagatcgaag
    22501 gccgccacgc cgccgatggg aaaaccgtaa ccccagtgaa catccggcat cgccagagaa
    22561 aaattcacaa tgcccggcag gtgcgcgacg ttggccacct gcttcgcgct ttcgtcgccc
    22621 tttaaaagct gaaagagttt ttcattggcg tagatgatcc ccggcacgcg catgccgccc
    22681 gtctgcggca acagccagcg gttgtcatcc aattttttca gtttaatatc agacataaca
    22741 aattctcttc aaatcgccat accgtcgaca agatttatgt cacttcgacc gaagggagaa
    22801 gtcttaatat tatcgtcttt aagattcctc accccgacac gtcgggattc ggaatgacat
    22861 taagttacgg attttgtcac acatcaaaaa ttattctcgc cttccagcca gattctactc
    22921 tttttacagt cgcgccggaa taggttaccg ctttaatctc ggttttaatc gaatgctttt
    22981 tgttattaaa cgattctccg gttaaccgtg cctgtaattt tttattggaa aactctataa
    23041 tttcgcaact acccgttatg aaatgttcgc cattgaacag atgaagcaac tctcgtaaaa
    23101 aattgatcag caaatcggca acatccgttc cttcgactgt tatttttttc tgccgctttg
    23161 ctgttgcccg gcctgctttg tgctcaatca tcacatcaaa caaagccccg gcggtattca
    23221 taaacagctc ttttcgggtc cggccataaa cttcaacgcc gatatcggct gtatggtcaa
    23281 agaatttgta gttcgacatg gagtgagtat agctgaatct tgcgtattga acaaggagag
    23341 ctgactgctc ggcaactttc atgacaagct gatcttgagt taaacaatag gtgttagccg
    23401 attgtttaac agtcacaatg atttaacatt aaatagaacc gtccccaatt tctctgaggg
    23461 actcctttct gatgtgcgtg ttggtcgtgc ttcacacact accagaaaat cccttcatgt
    23521 ccgttcttta tctatgagtg gtgcacttgc aagtagaatg caccttagct ggtcagcctt
    23581 ttctaatata atcatgtccc cttttcgagt acaaccgtcc ctattgtttt ctctccccag
    23641 attcaatgga ttcttcggct gcattagaga tgtcccagaa tcctttgctt gccctcgaca
    23701 actgttcatc gagcatgtcc caagcaaagc cagcgttccg cactaggaga cccataagct
    23761 ctaccagtct cttcattcga tcgatggtgg agcgcagatc accccatttg ataccaagcg
    23821 ttcctatatc cacgaactga tacttgtcgg caacatacct gacttctttg tgcgcagata
    23881 ccttatctcg aactattcga aaagctttga tcttcttgct ggtcgacaac tctgcccaga
    23941 ttgccattgc ctcacagtag agtttttcaa actgctctcg ccgttctgct tcctcacgca
    24001 gctcaattcg gtgaagtgcc tcaatgatct caggctctgt ctgctcttcg atggatggaa
    24061 tcttccatat agcgaatcga ttctgaagct ccttgcggat cacatcgtct gtgattgctc
    24121 gtattaggtt gtgtaggctg ggagttttat catcgtcgtc aagcgtcaag tttgcgatat
    24181 cctgcgaaca cgacagaaat aacgagtgtt taagtatgca aaatcctctc gcttgattgc
    24241 cggagcatcg ctctttcggc attgattcat caaagagcat aggctctaaa agagcgtacc
    24301 gttcgcgcag ttgaataaat gcatcgagta agtgggaagc gtgggcttca agcctttcaa
    24361 tatgagttgg cattggtctt tatcctacta acatggtatt caccgagcgt ggctctgctc
    24421 ttttgaaata acactttaat ttctattact cataaatgtc atcaacatag acatgtggtc
    24481 caacatagta tgcaaacaac agcaataaag atgcagcaag aattgttaat gaaacatgta
    24541 ttacagtatc ttcgatatta tcaagatgtg gttctttatc aataaagaga tatctcccac
    24601 aatttttaac tatggccaat acaatgaccg ctatcaacaa tattacaatg taatgactaa
    24661 accaagaacc taggaagcct agaaaaccat tttgccaagg agggttttta tattgaataa
    24721 tgtgtcttgc atagcaaaag aaaaatgctg aacatgaaag cactatacct tttatagaga
    24781 atagtgatct caatttattc atttttttat ttcctaaatt taacattgtc tagatggctc
    24841 ggctatgcca cggggtttta accctccgct tctcccacgt cgcgttgctc ctgggatagt
    24901 tcgctttacg cttcgagcgt cactacgctc gcttcaccac ggtgcaagac ctggtgtaat
    24961 tcgactaacg tttcgatctt cacttcattc gatctcaacg agtctcatta tcagcttggc
    25021 tcactactat tcctcagcac cgttgtcttc gccgttggtg tcagc
//
LOCUS       DAONQZ010000030        24964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2214_length_24964_cov_16.107712, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000030 DAONQZ010000000
VERSION     DAONQZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24964
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_24964_cov_16.107712"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..803
                     /locus_tag="PLR13_06560"
     CDS             87..803
                     /locus_tag="PLR13_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQC19035.1"
                     /translation="MNWTKDETVAIITMTNGENRQNLTFAKTLNAMLDEIIADKTITA
                     LIITSNDPKNFSQGIDVEWIMGQMQKNEHQPVTDFINEMDVVFKKLLLYPIPVIAAIN
                     GHAFGNGSIIACACDFRFMRSDKGFFCFPEVNISIPFRWGMNAFVKKAIPIHLLSQMQ
                     LTGNRFTAPELEKHNIIVKACANQEELMSSAIAFAKTFQKKRTIFGELKRRLYADIIK
                     VLDDDFMPPTSKITSLVMMD"
     gene            844..1827
                     /locus_tag="PLR13_06565"
     CDS             844..1827
                     /locus_tag="PLR13_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQC19036.1"
                     /translation="MKTAITEMFSVKYPIICGAMMWLCRPVLCAAISNAGGLGNLTAG
                     NYPTEDEFRDAIRQTRKLTDKPFMVNVTILPSVHVTAEHHKMYLRVCAEEKVAGLEIS
                     GTPLDKAVGKEFIDILKKAGVKLFHKVGSVRHAVHAEKTGYDGIYAAGIEEGGHPLND
                     DVTTMVLTPRIAETVKIPVVTVGGVADGRTLAAALALGAQGVMMATRFVATKECEVHD
                     NIKQEIIQRQEFETALICKSIGLQGRAIRNKLVEDVLAVESTGGGLEKLIPLIAGERV
                     KKAWEFGDVDSAPMMMGQSVGLVKDIPTCKELMERLVAEAQADIERVRNIF"
     gene            1891..3255
                     /locus_tag="PLR13_06570"
     CDS             1891..3255
                     /locus_tag="PLR13_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQC19037.1"
                     /translation="MSGVTTKETALATKLSKPEPRDETVPGSNAHGRFVSREIIALMG
                     KDDITSVSLGDHIEKTMTILFADIRGFTSLSEEMPPRENFTFINAYMKCMNPVIARHG
                     GIINKYIGDAIMALFPADADDAVKCAIGMLRQLVAFNRSIKKTGSSRIKIGIGLNTGL
                     MMLGIVGGEHFMETTVISDAVNLASRIESMTKDYNVPFLISEHTYYSLFGASEHNTRF
                     VDRVRVKGKAQCQSVYEVFDADPKSLRTAKQKTKADFEEALAFYHFKDVPKATKLLEK
                     LLRRVPGDTVAKVYHERCLRFLKTGIHESSGEFDLAISWNKSMAIDHSLIDEQHRDLF
                     IHTREFVEAVKTSKNYSQLRNVLEFMDKYIQVHFRTEERLMAQKNYPFLQMQVDQHRR
                     FSRYFDGLRNEIKRNFETNRIFILFRIQVLIVDWLINHTSKLDLHFGKFLRYRKGRGL
                     KSLS"
     gene            complement(3279..3683)
                     /locus_tag="PLR13_06575"
     CDS             complement(3279..3683)
                     /locus_tag="PLR13_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19038.1"
                     /translation="MTQNKIVAHYQDGNLFKGITNDFSPNKELFHLFERAAQEDNRPL
                     TLKMDGLKGLFYVKDYDGNPSYHEQKEFDPSRPLAGRKIKVIFKDGELMVGTTNGYQP
                     NRPGFFLVPADANSNNERCFVVSSAATEIAFI"
     gene            complement(3721..4044)
                     /locus_tag="PLR13_06580"
     CDS             complement(3721..4044)
                     /locus_tag="PLR13_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19039.1"
                     /translation="MKNHENYVKGILEQNPDAEKLKELLAYHDKQIKWIQHERLVHLI
                     VMLFVCLFTMMIFGFAVIRPSLSVILLFVILLILSAAYLLHYFRLENGVQKWYVIAGE
                     IRRQF"
     gene            4234..4647
                     /locus_tag="PLR13_06585"
     CDS             4234..4647
                     /locus_tag="PLR13_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQC19040.1"
                     /translation="MVNKVILIGRLGKDPEVRYTPDGTMVTNFNLATDEQRKDKNGEK
                     IQKTEWHKIVTWGKLAEICGNYLVKGKLVYVEGRIQTRSWEDKEGVKRYTTEIIASDM
                     RMLDSKGQTKSGETSFDADAANVNDGSVPLDDVPF"
     gene            4797..6362
                     /locus_tag="PLR13_06590"
     CDS             4797..6362
                     /locus_tag="PLR13_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19041.1"
                     /translation="MKNINVLMIQPKTPQTFWSHDHTIKALGFKATMPPLGLLTVAAM
                     LPQNFNVKLVDLNIEDLDMRDVDKSDMVFLTGMIVNRESFMEMVHICQKKGKIVVAGG
                     PFVQSVYGTKGSNDLEISKIDHLILYEAENNLPQFLDDYVQGKAKRVYDNNEKPSLTQ
                     TPIPRFDLIRPRDYVSMALQFSRGCPFNCEFCDIIQMFGHIPRTKTPEQFMGEVEALY
                     STGYRGRLFVVDDNFIANRKKVKELLRLLAAWQEERKYPFLLFTEASIDLAKDDELLG
                     LMRRSGFTSVFIGIESPDEKTLRATNKNQNVTCDMNAAIAKVQKAGIEVMGGFILGFD
                     TDADDIFDSQLEFIRKNSIVQSMVGLLAAMPHTDLYNRLEREGRLVLGVNHSGNNVDT
                     DLNFIPKMPVKQLIEGYRRVIKETYSPRNYFARASTLLKKFPDMRMSKLKYAEPWLKK
                     LYMSKMTSHPNKSKIVFEIMKLVFKPFGIYGLVFIIKSLRFGLLSMPVAIELTFRGLH
                     YMSVAREIENAAD"
     gene            6591..8486
                     /locus_tag="PLR13_06595"
     CDS             6591..8486
                     /locus_tag="PLR13_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC19042.1"
                     /translation="MKRMIPLFLLLVCMTLAGCAGVKVSFVGSGDYMSLRRGDILTTG
                     ELSTYTGASLQVVGIDKKVCDKDASGCRKALMETIGLHSENRLSALAELWLKDAMELE
                     KQFIESGHIEATLNAYLETARYSYAYLFLTKRTPNMRALEDRQTQVRDYYNYAVEKTL
                     TTLFEYYRKRLPPDFNFEKDTHFESGQWHITIRNEDLRLAEEHIFPEDLIPASSLSFA
                     GLRNQYRRDGLGAELVAVTSTRVVSRQSAEMPFSETPFPPVTAVMSFPGNTWMDVLKT
                     NEVVITGYDPYSRESFKLVGKEVPLAANFTSGYGLWLARSGFAAQSLLTLIGKGDVLE
                     MPRVYLMQPYNPQRHIVIMLHGLASSPEAWINLANEVLGDETLRKNFQIWQVYYPTNL
                     PIAFNHYEISKAITQTLKHFDPEGKARASQKILLIGHSMGGILSRLMVSSSGDRLWGA
                     LLKKYPLEGRRLERVRRELGPYMSFEPLPQISRAIFVAAPHKGTPVAEIALVRWIANF
                     IKLPFSVLGRFREVAQLLVDPGSANPVSLTRSFNSIDNLSNRDPFIQLTSTLPVSSRV
                     RYHSIIGNNTPKKPLAESSDSVVPYSSSHLEGAVSEKVIASGHSVQEMPDAIIEIRRI
                     MHLHLKE"
     gene            complement(8652..9587)
                     /locus_tag="PLR13_06600"
     CDS             complement(8652..9587)
                     /locus_tag="PLR13_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19043.1"
                     /translation="MNVNKFIDDKVRDIRQAVGDGFAINALSGGVDSAVVTALGHKAL
                     GKRLKTYIIDNGIMRENEPAKVAAAFKKLGIQVDVFDASRAFFSALKGLTDPEEKREA
                     LTQTFYKNVFGKLVVKSKARYLLQGTILTDVDETVAGIKRQHNVFEQLGIDPQKAFGY
                     KILEPLIELRKDGVRKVGRGLGLPEDMFARIPFPGPALAARIIGEVTPARIALVKKAT
                     AITEAALKDVKAFQYLAILHEDRVTGMRNNKRVFGNQIEIRCWNSLDARTARPTRLSY
                     AILEGIAAKITNEIPDVVSVTYNITSKPTSTIEAI"
     gene            complement(9717..10094)
                     /locus_tag="PLR13_06605"
     CDS             complement(9717..10094)
                     /locus_tag="PLR13_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HQC19044.1"
                     /translation="MTSRRAKIGLGYHLAAFAAVNAVLVWINLDTSPEYFWAKWPLAG
                     WAVALSYHAFSVFSSLIKAHKGFYYHLFSFLIINAFLIFINFDLYPQYLWFKFPLIVW
                     TIMIVFHGWRVFSERQKAKAVAA"
     gene            complement(10177..10389)
                     /locus_tag="PLR13_06610"
     CDS             complement(10177..10389)
                     /locus_tag="PLR13_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19045.1"
                     /translation="MKEKHAIVKLVFGCIDDVVSTTESIHKSIAENVKGVSRSGFAEG
                     GKRKHVYDTIKAVNGKVEELVSGLKK"
     gene            10627..10845
                     /locus_tag="PLR13_06615"
     CDS             10627..10845
                     /locus_tag="PLR13_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19046.1"
                     /translation="MMAIIRKELKEKSKNVLLRVSIPAPLMAEIKKIKKLCRDTGFYF
                     DIKPDVISAVEQAVEEAKALIQKEKKSR"
     gene            10894..11757
                     /locus_tag="PLR13_06620"
     CDS             10894..11757
                     /locus_tag="PLR13_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HQC19047.1"
                     /translation="MENLKKGKKALVVEGGGMRGVFAAGVLNAFGSGGFDPFDMYLGV
                     SAGACNLASHLAGQNDRNYDIIKRYSIDGRFINLGRFLRGGHLMDLDWLWDVTISEYR
                     LDLKKLFGRLKEREKEYIIAVTSVATGKSAYLEPDEDTLEHYLKVSSSLPIFYRNFLE
                     VHSEKMTDGGVTDSLPVQEAIRRRATQITVIRTRPSQYIKKKSMFSMCYPIIFRDYPL
                     FAQAMKKRPGNYMESVRLIRHPPAGVRVFEIAPPPDIDVGRMTTDIKRLRAAYQSGRD
                     HGRKFMDAHAP"
     gene            12068..14299
                     /locus_tag="PLR13_06625"
     CDS             12068..14299
                     /locus_tag="PLR13_06625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQC19048.1"
                     /translation="MEQKHLAPFTPELTQNALTVLERRYLKRDKSGKILETPSEMFYR
                     VADTIAAADKKFNKKADTRKRAEEFYAMMASLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMDEIFDAVKFTALIHKSGGGTGFAFSRLRPANDVVLTTTGISSGPISFMRVFDV
                     ATETIKQGGTRRGANMGILRVDHPDIMDFIMCKADKKQLNNFNISVGLTEAFMTAVER
                     DENYDLINPRDKQVTGHLNARKVFNRIINQAWENGEPGIVFLDRLNRDNPTPQAGEIE
                     STNPCGEQPLLPYESCNLGSINLAKMVKDGKIDWARLKEIVRLAVHFLDNVVEVNKYP
                     LPQIAQMTMANRKIGLGVMGWADMLIKLGIPYNTDEAMELAEKVMKFINEQGHGASQQ
                     LARKRGPFPNFKGSIYDKKGQPPLRNATVTTIAPTGTISIIANASSGVEPLFAVSYVR
                     QVMDNDILVEVHPYFEALAKERGFYSPELMQRIAEHGTIHDLEEIPEDIRQYFVTAHE
                     ISPDIHIRMQAAFQKYTDNAVSKTVNFKKEATVQDVARVYELAYKLDCKGVTIYRDGS
                     RDQQVLSKGKKEETAVQEDADREQHLTKRERPKILKGWTYQMQTGCGPLYITINEDDK
                     GLFELFTTMGKSGGCAASQSEAIGRMVSLAWRSGLSSRQVIKQLQGISCHSPSGFGEN
                     KILSCADAVARAIQSHIEANGGKVQHEKIVFRKGACPDCGGIVEHEGGCAVCRMCGYS
                     ECA"
     gene            complement(14372..16459)
                     /locus_tag="PLR13_06630"
     CDS             complement(14372..16459)
                     /locus_tag="PLR13_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC19049.1"
                     /translation="MEKFFYPRSMVVIGASVKRLNLGHIIVLNNRQHNYEGNIYGVGR
                     EEGDIAGAPVYDSVEKIPEVPDVAIIIAPAETVPDFMDACGKKGIKRLVIESGGFSEY
                     SQGQNVLEKKVLEIADRYGMRFIGPNCIGTINFDIKMMMPFGFFPRTAPGGRVAMIVQ
                     SGGVGGSYLGEFGDNAIIPGKFAAIGNKLQIDEVDVLEYLLNDDKTDVVTMYLEGFKR
                     GRAFFDLAVQSDKPLIVLKSNRSDLTAKIAQSHTTALSADDQVVDGAFRQTAVIRVDD
                     DIDFINAVKIIRLPLMKSRRVAILSRSGGHAVMSVDACAKFGFSLVDFPPAFIENLKT
                     IYNTRVIAHQNPLDLGEIFDYTIFTQILEEALKLPDVDGVLFNHLYIADYEGKMSRDF
                     LSNVGKLVQQYQKPVAMTMVTDTQELVNISKNQSYPIFTTPLTAAKALNMSATYYEEK
                     TAREARGRAVQTTLDMAAIDIIQDKCKTQKRIPLTDESLRIVEAAGIKSIAGATVKSA
                     KEAGDVSLHFPVAVKLLSRDASHKSDIGGVRLNIKNQRELKKAISDIKKSLKEMKPRP
                     AMDGFLIQEMSADGVECFVGGRQDPVFGPIVIAGLGGIFLEIFRDTSIRLAPVTKSEA
                     LDMLKNLQAYPILQGARGKMKADIDAFADVICRVSNLLAAVPDIAEIDLNPVIVHEQG
                     KGVSIVDSRIFFK"
     gene            complement(16508..16897)
                     /locus_tag="PLR13_06635"
     CDS             complement(16508..16897)
                     /locus_tag="PLR13_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQC19050.1"
                     /translation="MQDTQETFTSIGEIAKKLDMSQRTIRYYEEIGLLNSIKRVEGGR
                     RIYTEADFRRLKLIKRLKIMGMTLSEMQELEAMWTIEKSNDKVLGRLLELLNNQLRRL
                     DDRIADLNILRNEIVEYQKRIKSKIGK"
     gene            complement(16913..18469)
                     /locus_tag="PLR13_06640"
     CDS             complement(16913..18469)
                     /locus_tag="PLR13_06640"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HQC19051.1"
                     /translation="MSNIINRDTIGDMARRAATKYGDKTAIIFRNIKLSFYDLEREAR
                     RFANLMTRYGVRKGDRVAVYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAFIIN
                     HSGSRLFIIEDTLYPVIAGCKDKFPAVEKWGFIPLNDSGIPDGFFNLIAEMSAMNYDE
                     PQVEVSAEDIVQIPYTSGTEAKPKGAMLSHRALMSQYHSCIFDGRYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPVEMIRLIEKYQITHMFAPPTVWIAILNHPE
                     FNNYNHGSLKKAAYGASIMPVEIIKQLSVTFKGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVALGDVGEIVHRSGHVMSGYLNDPEKTAEAFQFG
                     WFHSGDLGRFDNDGYLYVVDRKKDMIKTGGENVASREVEEVLYQYPGIEEVAVIGLPD
                     PKWIEVVAVVAVLKKGVSIQEKDIISFCKDRLAGFKCPKKVFITDRLPKNPSGKILKR
                     ELKEQLSSSI"
     gene            complement(18496..20136)
                     /locus_tag="PLR13_06645"
     CDS             complement(18496..20136)
                     /locus_tag="PLR13_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC19052.1"
                     /translation="MSNFVKKTIGQLVRETAECYPQNPALVCPDFGIRQTYSEFYQTC
                     RDVAKGLIALGIKRGDNVAVWTTNIPDWIHLQFALGMTGGILVTVNTNYQTHELEYIL
                     KQSDSTTLFLMESYREVNFYENVRKILPELDHHQPGTPVSAQLPALKNIIYIGKREST
                     PGMLHFSDIITMGREVTDKQLEDRLHSLHDDDVINMQYTSGTTGFPKGVMLTHHNIVN
                     NARMVGDVMGMTEKDKLLIQVPLFHCFGCVMSSLNCVYHGSTMVVVEYFDPLKAVQWI
                     SAEHCTAVNGVPTMFIAILNHPDFDKYDMSSLRTGIMAGAPCPVETMNQVRTKMHCPE
                     IVIAFGQTESAPVMTMTRRDDPVELRVSTVGRLLPDIEGKIVDPDLGIDLPPNAQGEI
                     VTRSECVMKGYYKMPDATADAIDRYNWLHTGDLGEMDDNGYFKVTGRIKDMIIRGGEN
                     IYPRELEEFLFTHPKVVNVQVIGIPDKKYGEQVLAAIQLKAGQTATPEEFTEFCQGKI
                     ARHKIPKYWEFVDDYPTTASGKIQKFKMKEVFEKKYPA"
     gene            20606..21322
                     /locus_tag="PLR13_06650"
     CDS             20606..21322
                     /locus_tag="PLR13_06650"
                     /inference="COORDINATES: protein motif:HMM:NF016459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQC19053.1"
                     /translation="MIPKIIHQTWKSTEIPPRWQRFRDAWKKFHPGWEFRLWTDEDNR
                     RLVERDYTAFLQLYDSYAYPVQKADFARILYLHKFGGVYADLDLEPLRPLDGIIDQPH
                     LILGEEKEGMGKSLNRCDFIINAFMASPKGHPFWEMLLERLRVTFRHKRLLQSKGHYI
                     IQTVIFELDKLARDYQQTHQDITIYPYEVFYPLSWHEACSELERSKAVEMSYTIHHFD
                     TTWFSGGMMLMKKLRRRLNI"
     gene            21404..22672
                     /locus_tag="PLR13_06655"
     CDS             21404..22672
                     /locus_tag="PLR13_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQC19054.1"
                     /translation="MEKTLLIIFLILFVGRILWRFILQQLNIRHLRQCGKIIPPAFRN
                     VIDQATLSKMADYTYDNLRLENKENSTEDLLELAIIFLFLPLLVAKTNGLQLHLIWQA
                     LVFFGTLALIGGVIGLPFDIYRTFVLEKKYGFSTITWKLWLTDLVKSVIISAILMGFL
                     LSVFMAFVLYLPTSWWFWAWIFFAAFEILLLWLFPVVIAPLFNKYEPVKDEELKEKIT
                     SLLSKVGLKAKGIFQVDEGKRSKHTNAYFTGIGKTKRIVLFDTLLASHTHDEILAVLA
                     HEIGHWKKKHILKQLIFMVAASFVGFYLVYLLVNWPPIYGAFGLPQTPVYAGLLLVSL
                     YFSCVGFFFSPLAAFISRRYEREADRMAHELTGTSEPMIHALKRLAKDNLSNLHPHPW
                     YVWFYYSHPPLIERIEYLKTMDKGNLQRQR"
     gene            complement(22679..23305)
                     /gene="thiE"
                     /locus_tag="PLR13_06660"
     CDS             complement(22679..23305)
                     /gene="thiE"
                     /locus_tag="PLR13_06660"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HQC19055.1"
                     /translation="MRTFLRHGLYLILTDPRDGYETLCRWAVEAGLPAVQLRYKGHNE
                     REHLALARALRQITKGTGTLFIVNDRPDIALISQADGVHLGQDDLPAAEVRQLIGPTM
                     LLGLSTHNLEQVAAANAVPVDYIGFGPLFATNSKERLAPVTGPDILKAALAISRYPIV
                     AIGGLTLERIRQLQPRPHNTAVIRAVNDASDPLAVMRAIHESCMNRTS"
     gene            complement(23326..24096)
                     /locus_tag="PLR13_06665"
     CDS             complement(23326..24096)
                     /locus_tag="PLR13_06665"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HQC19056.1"
                     /translation="MLNEIIISRAILDSYFKKLGSCLDLDVAIVGGGPSGLVAGYYLA
                     RAGRKVALFERKLSIGGGIWGGGMMFNSIVVQEAGRQLLEEFDLQGREYTPGYYVLDA
                     VDITSTLIHKAVRAGLQVFNLIAIEDVVIKNERVAGLVINWGAVDTLKWHVDPLTVHA
                     RYVIDGTGHPANVTEVLVRKMGVRLNTSTGGIVGEKSMEAGKGELQTVENTREVYPGL
                     FVSGMAANAVYGGYRMGPVFGGMLLSGLKVAEEILARL"
     regulatory      complement(24178..24270)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(24324..>24964)
                     /locus_tag="PLR13_06670"
     CDS             complement(24324..>24964)
                     /locus_tag="PLR13_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQC19057.1"
                     /translation="LMIKTIFWDNDGVLVDTEGLFFRANQEILAGLGIPLQREQFEEI
                     SLVRGESVMRLAATPGTDAFIKLRRDRDALYADLLTRDPLVIDGVREVLDALQGKYLM
                     GIVTSSGHEHFEIIHRQSSLLPYFQFFLTLKDCPYTKPHPGPYLKAIALSGFAPEECL
                     VIEDSPRGLAAATSAGLRCIVIPSKFTCNHDFAGACKVLDSIKGLPAFLKEF"
BASE COUNT         6376 a         6060 c         6129 g         6399 t
ORIGIN      
        1 ttatgctgtt gaaatagttt aatattatag cctttgcggc cgtaattttt acattttgaa
       61 aaagagaggt aaaacatatg tctatcatga actggaccaa agatgaaacc gtcgccatta
      121 taaccatgac caatggtgaa aacagacaaa atctgacatt tgccaagaca ttgaatgcga
      181 tgcttgacga aataatcgcg gataaaacca taaccgccct gattattact tctaatgacc
      241 caaagaattt ttcgcagggc atcgatgttg aatggatcat ggggcagatg cagaaaaatg
      301 aacatcagcc ggtaacggat ttcatcaacg aaatggatgt ggtttttaaa aaattgcttc
      361 tttatccgat cccggtcatt gcagccatca acggacatgc tttcggcaat ggttcgatta
      421 tcgcctgcgc ctgcgatttc cgattcatgc gctccgacaa aggattcttc tgctttcccg
      481 aagtcaacat cagcatcccc ttccgctggg ggatgaacgc ttttgttaaa aaggccatcc
      541 cgatccactt gttgtcgcag atgcagttga ccggtaatcg tttcaccgcg ccggaactgg
      601 agaagcacaa cattattgtg aaggcctgcg ccaaccagga agaattaatg tcctccgcga
      661 tagcttttgc caagactttc cagaaaaaac ggacaatttt cggcgaactg aaaaggaggc
      721 tgtacgccga tatcatcaaa gttctggatg atgactttat gcctccgact tccaagatta
      781 cttcgctggt gatgatggat tagcattgaa caaaagttat gaccatttaa agaggtacaa
      841 aaaatgaaaa cagcgattac cgaaatgttc agcgtcaaat atccgattat ctgcggagcc
      901 atgatgtggc tttgcaggcc cgtactgtgc gctgccatat ccaatgccgg cgggctgggc
      961 aatctgacgg ccggaaatta ccccacggaa gacgaattcc gtgatgccat tcggcagaca
     1021 cgaaaactga cggataaacc ttttatggta aatgttacta ttctgccgtc agtccatgta
     1081 acagcggaac atcacaaaat gtatttaaga gtgtgtgctg aagaaaaagt cgccggtctg
     1141 gagatatccg gaaccccgtt ggataaggcg gtgggcaagg aatttataga cattctgaaa
     1201 aaagccggtg tgaaattatt ccacaaggtc ggatcggtgc gccatgccgt gcacgccgag
     1261 aagaccggct acgacggcat ttacgccgcc ggtattgaag aaggcggaca tcctctcaat
     1321 gacgatgtca ccaccatggt gctgacgccg cgtattgccg aaaccgtgaa aattccggtg
     1381 gtaacggtcg gcggtgtcgc tgacggacga acccttgccg cggcactggc tctcggcgcc
     1441 cagggcgtga tgatggcgac ccgttttgtt gccaccaaag aatgcgaagt tcacgacaat
     1501 atcaagcagg aaattatcca gcgtcaggaa ttcgaaacag cgctgatctg caaatcgatc
     1561 ggcctacagg gccgggccat cagaaacaaa ctggtggagg atgttctggc cgtggaatcg
     1621 acgggcggcg gactggaaaa actgattccg ctgattgccg gagagcgcgt caaaaaggcc
     1681 tgggagtttg gagatgtgga cagcgctcca atgatgatgg gacaatcggt tggactggtc
     1741 aaggatattc ccacctgcaa ggagcttatg gaaagactgg ttgccgaggc ccaagccgat
     1801 attgagcgcg ttagaaatat tttttaaaaa tatgtttgct cattagaaat gatggataga
     1861 actctgatgt ccgtttccgg gaaagtctga atgtctggtg tgacaacaaa ggaaacagcg
     1921 ctcgctacaa agctttcaaa accggaaccg cgggatgaaa ccgttccggg cagcaacgcc
     1981 cacggccgct ttgtgtcgcg ggaaatcatt gccctgatgg ggaaagacga catcaccagc
     2041 gtttccctcg gcgatcacat tgaaaaaaca atgaccatcc tttttgcgga tatcagaggg
     2101 tttacctctc tttccgaaga aatgcccccc cgggaaaatt tcacgtttat taatgcctac
     2161 atgaagtgca tgaatccggt gatcgcgcgt cacggcggta tcatcaacaa atacatcgga
     2221 gacgcgatca tggcgctttt ccccgccgat gccgacgacg cggtgaaatg tgcgatcggt
     2281 atgctcagac aactggtcgc ctttaaccgc tcgattaaaa aaacaggttc ttcccgtatc
     2341 aagatcggca tcggattgaa taccggcctg atgatgctcg gcattgtcgg cggcgagcat
     2401 tttatggaaa caaccgtcat cagcgacgca gtcaatctgg cgtcccgcat cgagtccatg
     2461 acgaaagatt acaatgtccc ttttctgatc agcgagcaca cctattacag tttgttcggc
     2521 gcatctgaac acaatacacg ttttgtcgac cgcgttcggg tgaaggggaa ggcacaatgc
     2581 cagtcggttt atgaagtctt tgacgccgat ccgaaatctt tacgcacggc taagcaaaaa
     2641 acaaaagccg attttgaaga agcgctggcc ttttatcatt tcaaagatgt tcccaaggcg
     2701 acgaagctgc tggaaaagct tctgcgacgg gtgcccgggg atactgtggc aaaggtttat
     2761 catgaacgtt gcctccgttt tttgaaaaca ggcattcatg aaagcagcgg ggaatttgac
     2821 ctggctattt catggaataa atcgatggcg atagatcact ccctcatcga tgagcagcac
     2881 cgtgacctgt ttattcacac ccgggagttt gtggaagcgg tgaagacaag caaaaattat
     2941 tcgcagctcc gcaatgtcct tgagtttatg gataaataca ttcaggttca tttccgaacc
     3001 gaagaacgtt taatggcgca aaaaaactat ccctttttgc agatgcaagt cgatcaacac
     3061 cggcgctttt cgaggtactt cgacggactg agaaatgaaa taaaaagaaa ttttgaaact
     3121 aacaggattt ttattctttt ccgcattcag gtgctgatcg ttgactggct tattaatcat
     3181 accagcaagc tcgacctgca tttcggcaaa ttcctgcgtt accggaaagg tcgtggactg
     3241 aagagcctgt cttaagagca gacggaaatc accgtcaatc agataaacgc aatttccgtt
     3301 gcggcggaag agacaacaaa acaacgttcg ttattggagt ttgcatcggc cggaacgaga
     3361 aagaacccgg gacggttggg ctgatagccg ttggttgtgc cgaccattaa ttccccgtct
     3421 ttaaaaatga cctttatttt tcgtcccgcc aagggtcggg aagggtcgaa ctctttttgc
     3481 tcgtgataac ttggattgcc gtcataatct ttaacataga ataaaccttt tagaccgtcc
     3541 atcttcaatg tcaacggtct gttgtcttcc tgagccgctc tctcaaacag atgaaaaagt
     3601 tccttattgg gggaaaaatc gttggtaatg cctttaaaca aattgccgtc ctggtaatgt
     3661 gccacgattt tattttgagt cattgttttc tccttacgat tataaaggga tagccgtcat
     3721 ttaaaactgc cgtctgattt caccggcgat cacataccat ttttgcacgc cattttcgag
     3781 ccggaaatag tgcagaagat aggcagcgga tagaatcagc aggataacaa aaagtagaat
     3841 aacggaaagg gaaggtctga taacggcgaa tccgaaaatc atcattgtga aaaggcaaac
     3901 gaagagcatc acaattaaat ggaccaggcg ttcatgctgt atccatttga tctgtttgtc
     3961 gtggtaggcg agaagctcct ttaatttttc ggcatcggga ttttgctcca gaatgccttt
     4021 tacgtaattt tcatgatttt tcatgtaaga aatcattgcc aatcccgcaa atatttttat
     4081 gaatattaac atattttaat gaaaatacag atatcttttt taagattgcc gtaaattaat
     4141 gcggaagaat cgatacttat aaaattcgtt tgccacaatc tccctgtaat gttatgtatc
     4201 ccccgtaagg gcagatcaca aggaggaagg aagatggtaa ataaggttat cttgattgga
     4261 agattgggca aggatccgga ggttcgctac acgcctgatg ggacgatggt taccaacttc
     4321 aatttggcca ccgacgagca gagaaaagat aaaaacggcg aaaaaattca aaaaacagaa
     4381 tggcataaaa ttgtaacatg gggaaaactg gctgaaatct gcggtaatta tctggtgaaa
     4441 gggaagctcg tttatgtgga aggtcgcatt caaacgcgtt cctgggaaga caaggaaggc
     4501 gtcaaacgtt atacgacaga aattattgcc agtgatatga ggatgctcga ttccaaagga
     4561 caaaccaaat ccggagagac ttcttttgat gctgatgccg ctaatgtgaa tgacggcagc
     4621 gtgccgttgg acgacgtgcc cttttaatat gcggtaattt atgaaaaaga accgtttgga
     4681 aagttaatgc acgatccttc tccatcctat actttttaaa ttttctgcgc atatcttttc
     4741 aaaaaaccga cggggataat gtttttttat gcttattgcc cgccatagga aaaattatga
     4801 aaaatatcaa tgtgttgatg atacaaccga agacgccgca aactttttgg agccatgacc
     4861 ataccattaa ggctcttgga ttcaaagcca ccatgccgcc actgggattg ctgacggtag
     4921 ctgccatgct tccccaaaac ttcaacgtta aattagtgga tctcaatatt gaagatctgg
     4981 acatgcggga tgtggataaa agtgacatgg tttttctaac cggtatgatc gtcaacagag
     5041 agtcttttat ggaaatggtc catatatgcc aaaaaaaagg caagatcgtt gttgccggag
     5101 gtccttttgt ccaatcggtt tacggcacga agggaagcaa tgacctcgaa ataagcaaga
     5161 tcgatcacct gattttatat gaagcggaaa acaatcttcc gcaattcctt gatgattacg
     5221 tacagggcaa agcgaagaga gtttacgata acaatgaaaa accttccctc acacagaccc
     5281 cgattccccg ctttgattta atacgtccga gggattatgt ctccatggcc cttcagtttt
     5341 ccagaggctg tcctttcaat tgcgaatttt gtgatatcat tcagatgttc ggccacatcc
     5401 ccagaaccaa gacaccagag caatttatgg gagaagttga ggcgctttat tcaaccggat
     5461 atcgcgggcg tctttttgtt gtggatgaca acttcatcgc caatagaaaa aaagttaaag
     5521 aattgctcag attgctggct gcgtggcagg aggagcgtaa atatcccttt ctgctgttta
     5581 ccgaagccag tatcgacctt gcgaaggatg atgaactgct ggggctgatg agaaggtcgg
     5641 ggttcacctc cgtttttatc gggattgagt ctcccgatga gaagacgctc cgtgccacta
     5701 ataagaatca aaacgtgacc tgcgacatga atgctgccat agcaaaggtg cagaaagcag
     5761 gcatagaagt tatgggagga tttatcctgg gattcgatac cgatgcggac gacatattcg
     5821 acagccagtt ggaatttatc cggaagaaca gcattgtcca gagcatggtg ggtcttcttg
     5881 ccgccatgcc gcataccgat ttgtacaatc ggttggagcg ggaaggaagg cttgtccttg
     5941 gtgttaatca tagcggaaat aatgttgata ccgacctcaa cttcattcct aaaatgccgg
     6001 tgaagcagct tattgaaggc tacagaagag tcatcaaaga aacctactcg ccccgtaatt
     6061 atttcgcccg cgcgtcaacg cttttgaaaa aatttccgga tatgaggatg tcaaaactga
     6121 agtacgcgga accgtggtta aaaaaattat atatgtccaa aatgaccagt catccgaata
     6181 agtcaaagat agtctttgaa attatgaagc ttgtatttaa acctttcggc atttatggat
     6241 tggtttttat catcaaatcg ttacgattcg gattgctttc catgcccgtc gcgatcgaac
     6301 tgactttccg cggcctccat tacatgagcg tcgccagaga aattgaaaac gcggctgatt
     6361 aacggtctga agcagcaacg gtaaaaaaag accctgtatc cggaaggcgt gccggatcat
     6421 cagccgtggt ttcagggcgg gacgcccggt gaatgaacaa aaaatgtcct tgcccgtttg
     6481 gtacggtttt ctttgccgat tgtcaccctt aggattttct tgacccttac gaaattcatt
     6541 tatatattca gacaataaac cctaacgact tttcaatgcg gagccctcac atgaaacgaa
     6601 tgattcctct gtttctttta ctggtctgca tgacgcttgc cggttgtgcc ggtgtcaagg
     6661 tgtcgtttgt cggcagcggc gactacatgt cgcttcgccg tggtgacatc ctgacgaccg
     6721 gtgaattgag cacctacacg ggcgcctctt tgcaggtggt cggtatcgat aagaaagtat
     6781 gtgacaagga cgcttccgga tgccgcaagg cgttgatgga aaccatcggg ttacacagtg
     6841 agaatcgttt atcggcgctg gctgaactct ggctgaaaga cgccatggag ctcgaaaagc
     6901 aattcatcga aagcggacat atcgaagcga ccctgaatgc ttacctggaa accgcgagat
     6961 actcctatgc ctatcttttt ctgacaaaac gcacgcccaa tatgcgggcg ctggaggatc
     7021 gacagacaca ggttcgggac tattacaact atgccgttga aaaaactctg acaaccttat
     7081 ttgaatatta tcgtaaacgt ctgccgcccg attttaattt tgagaaggat acgcattttg
     7141 aatcgggaca atggcacatc accattcgaa atgaggacct gcgtttggct gaagagcaca
     7201 tttttccgga ggatttaatt cccgcatctt cactgtcgtt tgccggtctt cgcaatcaat
     7261 atcgccggga cggcttgggg gcggaactgg tggccgtgac ctccactcgc gtcgttagcc
     7321 ggcaaagcgc agaaatgccc ttcagtgaaa cgccctttcc tccggttact gctgtgatga
     7381 gttttccggg gaacacctgg atggacgtgt tgaaaaccaa cgaggtggtc atcacggggt
     7441 acgatcctta cagccgcgag tcgttcaagc tggtcggcaa ggaggtgccg ctggcggcca
     7501 atttcacgtc cggttacgga ctgtggcttg cccgatccgg ctttgccgct cagtctcttc
     7561 tgacgcttat cgggaaaggc gatgtgctgg agatgccgcg cgtttatctc atgcagccat
     7621 acaatcccca acgccacatc gttatcatgc tgcacggtct ggccagcagc ccggaagcgt
     7681 ggatcaatct ggccaacgag gtgttgggcg acgagactct gaggaaaaat tttcagatct
     7741 ggcaggtgta ttatccgacc aatctgccca ttgcgtttaa ccattatgaa atcagcaagg
     7801 ccatcacaca gaccctgaaa cattttgatc ccgaaggaaa agcgcgggcg tctcagaaaa
     7861 ttttactgat tggacacagt atgggcggga ttctatcccg gttgatggtt tcttcctctg
     7921 gagaccggtt gtggggtgca ttgcttaaaa aatatcctct tgaagggaga cggctcgagc
     7981 gtgtccggag agagctggga ccctatatga gttttgaacc gctgcctcag atcagccggg
     8041 cgatctttgt cgccgcgccg cacaagggga cgcctgtcgc ggaaatagcg cttgtccgct
     8101 ggatagccaa tttcatcaaa ctgcctttct ctgtgctggg caggttcagg gaagtggcac
     8161 aactgctggt cgatcccggt tccgccaatc ccgtctcgct gacccggtca ttcaacagca
     8221 tcgacaatct cagtaaccgc gaccccttca ttcagctgac ctccacgttg ccggtttcat
     8281 cccgggtgcg ttaccattcc attatcggta ataacactcc gaagaagcct ttagccgaat
     8341 ccagtgacag tgtggtgcct tattccagct cgcaccttga gggtgccgtc tcggaaaagg
     8401 tgattgcttc cggacatagc gtgcaggaaa tgccggacgc tatcatcgag atccgccgga
     8461 tcatgcatct gcacctgaaa gaataaaaaa acatcggcaa gacctgttga aaatagtttt
     8521 gcgatgacct tgcctggtct cccgccgcgg ccggttggac gtgttatctg gatgatgcat
     8581 cccgacccgc gatgaaaaaa atcccgatga cagggcgtta atttttttaa ataaattttc
     8641 ggttttccct gctaaatcgc ttcaatggtt gatgtcggtt tggacgtgat gttgtaggtg
     8701 acgctgacca cgtccgggat ctcgtttgtg attttcgcgg cgatgccttc cagaatagca
     8761 taggacagtc tggtcggccg tgcggtgcgt gcgtcaaggc tgttccagca ccggatttca
     8821 atctgattgc cgaatacgcg tttgttgttg cgcatgccgg tgacgcgatc ttcatgcaaa
     8881 atggccaaat actgaaacgc cttgacatct ttcagcgccg cttcggtgat cgcggttgct
     8941 tttttgacca gagcgattct ggccggtgtg acttcgccga taatcctggc tgccagcgcc
     9001 ggtccgggga aggggattct tgcaaacatg tcttccggca gacccagccc tcggccgact
     9061 ttccggacgc cgtccttgcg cagttcaatg agcggttcca gaattttgta gccgaaagct
     9121 ttttgcggat cgatacccaa ttgctcgaaa acgttgtgct gtcttttgat gcccgctacg
     9181 gtttcatcca cgtccgtcag aatggtcccc tgcaacaggt atctggcttt gctcttgacg
     9241 accagcttgc cgaaaacgtt tttataaaag gtttgcgtaa gggcttctct tttttcttcc
     9301 ggatcggtca gccccttgag agccgagaaa aacgctctgg acgcgtcgaa aacgtcgacc
     9361 tgaatcccaa gctttttaaa ggccgccgcc actttggccg gctcattttc ccgcataatg
     9421 ccgttgtcaa tgatatacgt tttcaggcgt ttacccagcg ctttatgtcc cagggccgtc
     9481 acgaccgcgg aatcgacgcc tcccgacagg gcgttgatgg cgaatccatc gcccacggcc
     9541 tgccggatgt cccgtacttt atcgtcgata aatttattta cgttcatatt ttttgccttt
     9601 atttctttca tcttgatcat ggcgactcct ttgaggatag aattataatg caaaattctt
     9661 taaacgaaat cgcgccgggt ttcaagtaat aaagcataag aagaaaaaat agtttcctag
     9721 gctgcgacgg ctttagcttt ttggcgttct gaaaaaactc tccacccgtg aaaaacaatc
     9781 ataatcgtcc acactattaa tggaaattta aaccagagat attgggggta taagtcgaaa
     9841 ttaatgaaaa tcaaaaaagc attgatgatc agaaatgaaa aaagatgata atagaatccc
     9901 ttgtgagcct ttattaaaga cgaaaagaca ctgaacgcgt gatatgaaag ggctacggcc
     9961 cacccagcaa gaggccactt ggcccagaaa tattcaggcg acgtatcaag attgatccag
    10021 accaatacag cattgaccgc agcaaacgct gctaaatgat accctaatcc gatttttgct
    10081 cttctgcttg tcatacaaat ctcctctcct atttttttgc gactgttgtc agacatgcat
    10141 caaagtcgtg cctgcgatcc gtgtccgaga aaatccttat ttctttaatc ctgagaccag
    10201 ttcctcgact ttcccgttaa cggccttaat ggtgtcatat acgtgtttcc gttttccgcc
    10261 ttccgcaaaa ccgctgcggg aaacaccttt tacgttctcg gcaatgcttt tgtgaatgct
    10321 ttcagttgtt gaaaccacgt catcaatgca accgaaaacc aatttgacga tagcgtgctt
    10381 ctctttcata agacctcctt tttaaaatca ttaaccgcag gccggcatca tcgtgccgag
    10441 ctcgatgatg caaatccttt tctccgaccg gcaaatgtaa tatacacaca gcaattataa
    10501 ttgtcaataa aaaaagcaaa ataaatagca acaattataa ctataaaata tggctattta
    10561 tattgacaag atatgagtat tatattatag caataataaa aattcagaac atgggttcag
    10621 ggaggcatga tggctattat aaggaaagag ctaaaggaaa aatcaaaaaa cgtattgttg
    10681 cgggtcagca taccggcgcc gctgatggct gaaatcaaga agatcaaaaa actctgcagg
    10741 gatacgggtt tctattttga tatcaaaccc gatgtcatca gcgcagttga acaagccgtt
    10801 gaagaagcga aggcattaat ccagaaagag aaaaaatcca gatagatcaa tacgcaccgg
    10861 tgaccggaat taagacgagt gacaagcaca acgatggaaa atcttaaaaa aggcaagaaa
    10921 gcgctggttg tcgaaggcgg cggcatgcga ggcgtttttg ccgcgggagt tctcaacgcc
    10981 ttcggcagcg ggggattcga tccgttcgat atgtatctgg gcgtttccgc cggcgcctgc
    11041 aatcttgcct cgcatctggc cggccagaac gacagaaatt atgacatcat caaacgctat
    11101 tccatagacg gccggttcat caatctggga agatttctgc gcggcggaca cctgatggat
    11161 ctggactggc tgtgggacgt taccatcagc gaatatcgtc tggacctgaa gaaactattc
    11221 gggcgattaa aagaacgaga gaaagaatac atcattgcgg taacatctgt cgcgacggga
    11281 aaatcagcgt atctcgaacc ggatgaggac acgctggaac attatctgaa agtgtccagc
    11341 tcgctgccga tattttatag aaattttctg gaggtccatt ccgaaaaaat gacggacggc
    11401 ggcgttacgg attctctgcc ggtgcaagaa gccatccggc gcagggcaac ccagataacg
    11461 gttatcagaa ccaggccgtc gcaatacatc aagaaaaaga gtatgttttc catgtgctat
    11521 cctattattt tcagggatta tccgctgttt gcgcaagcga tgaaaaaaag acctgggaat
    11581 tacatggaat cggtgcgttt aatcagacat ccaccggcgg gagtgcgcgt ttttgaaata
    11641 gcgccgcccc cggacatcga tgtcggcagg atgacgaccg atatcaaacg tcttcgcgcc
    11701 gcctatcaaa gcggcagaga tcacggcaga aaatttatgg acgcgcatgc gccttgaaaa
    11761 ttgcccgatc agaccgtctg acaaattatc aggacatcgg aaatcgtaaa cctgaacaaa
    11821 gcaaagactt gatctccata agccttcacc ggacggatga acatttataa tgtaatgaac
    11881 gtgcggatgg ccggttaaag agccttttgc ttgttattta tttttattct caactccttg
    11941 aatttcttca tttaaaattt tcttccgtct cttgacttta tctatatatt gtggttaata
    12001 aatcaaattc atacaatata tagtatcttt ttctttatct ctttcgatat ttatgaggaa
    12061 tcgctttatg gaacagaaac atctcgcccc ctttactccg gaattgacgc agaatgcttt
    12121 aaccgttctg gaacgacgat atcttaaacg cgacaaatcg ggaaaaattc ttgagacgcc
    12181 ctcggaaatg ttttatcgcg tggcggacac gatagccgcc gctgacaaga aatttaacaa
    12241 gaaggcggat acgcgtaaac gcgccgaaga attttacgcc atgatggcct ctctggaatt
    12301 tctgcccaat tcgccgacct taatgaatgc cggacgcgaa ctgggacaat tgtccgcctg
    12361 ctttgttctg ccggtgggcg attccatgga tgaaattttc gacgccgtga agttcaccgc
    12421 actgattcat aaatcaggcg gcggcaccgg atttgctttt tcacgcctgc ggcccgccaa
    12481 tgatgtcgtt ttaacgacaa ccggtatttc cagcggcccg atttcattta tgcgtgtttt
    12541 tgacgtagcg acggaaacca tcaaacaggg cggcacccgg cgcggcgcca atatgggcat
    12601 tctgcgggtg gatcatccgg atatcatgga tttcatcatg tgcaaggccg acaaaaaaca
    12661 attgaacaat ttcaacattt ccgtcggtct gaccgaagcc tttatgacgg ccgttgaaag
    12721 agacgaaaat tatgacctga tcaatccgcg ggataagcag gtcaccggac acctcaatgc
    12781 gcgcaaagtt ttcaacagga ttatcaatca agcctgggaa aacggcgagc cgggcattgt
    12841 ctttctcgac cggctcaacc gcgacaatcc aaccccgcag gcgggcgaaa tcgaatcaac
    12901 caatccctgc ggcgagcagc cgcttctgcc ctatgaatcc tgcaatctgg gttccatcaa
    12961 tctggccaaa atggtgaaag acggcaagat cgactgggca agactcaaag agatagtgcg
    13021 ccttgccgtg cattttctgg ataacgttgt cgaagtgaat aaatatcctc tgccgcagat
    13081 cgcccagatg accatggcca accgtaaaat tggcctgggc gtgatgggat gggcagatat
    13141 gctgattaaa ctgggaattc cctacaatac ggatgaagcc atggaactgg ctgaaaaagt
    13201 aatgaagttt attaacgagc agggacacgg cgcatcgcag caactggcca ggaagcgcgg
    13261 tccatttccc aatttcaaag gttccattta tgataaaaaa ggacagccgc ccctgcgcaa
    13321 tgccaccgtg accaccatcg cacccaccgg caccatatcg attatcgcta atgcttcttc
    13381 cggtgtggaa ccgctttttg ccgtttctta cgtccgtcag gtcatggaca atgacattct
    13441 ggtggaagtt catccctact tcgaagctct ggccaaagaa aggggattct actcgccgga
    13501 attaatgcag cgtattgccg aacatgggac tattcatgat ctggaagaaa tccctgagga
    13561 tatccgccaa tattttgtca cggcgcatga aatatcgccg gacatacaca tcaggatgca
    13621 ggccgctttt cagaaataca ccgataacgc cgtcagtaaa accgttaact ttaaaaaaga
    13681 ggcgaccgtc caggacgtgg cccgggttta tgagctggcc tataaactgg attgcaaggg
    13741 cgtaaccatt tatcgcgatg gatccagaga tcagcaggtt ttgtccaaag ggaaaaaaga
    13801 agaaacagct gtgcaggaag atgccgaccg ggaacaacat ctgaccaagc gcgaacgtcc
    13861 gaaaatcctg aaaggctgga cctatcagat gcaaaccggt tgcggaccgc tttacataac
    13921 cattaacgaa gacgacaaag gtttgtttga acttttcacc acgatgggaa aatcgggcgg
    13981 atgcgcggca tcacaaagcg aagccatcgg acgcatggtt tctctggctt ggcgcagcgg
    14041 actgtcgtcc cgtcaggtga ttaagcagtt gcagggcatt tcgtgtcatt cgccgtccgg
    14101 tttcggtgaa aacaagattc tgtcctgtgc cgacgctgtg gccagagcca ttcaatccca
    14161 catcgaagcc aacggcggga aagtgcagca cgaaaaaatc gtattccgca aaggcgcctg
    14221 tccggactgc ggcggcattg tggaacatga aggcggttgt gctgtttgcc gcatgtgcgg
    14281 ttacagcgaa tgcgcataac cgatgcatcg ggacgatgcg tcaacgaccg tcatcaacaa
    14341 tgcattgccg ggctattcca ctgtcacgtt cttatttgaa gaagatgcgg ctatcgacaa
    14401 tcgaaacgcc cttgccctgt tcgtggacga taaccggatt caaatcgatt tcggcgatgt
    14461 ccggaaccgc tgccagaagg ttggacacac ggcaaatcac atcggcaaaa gcgtcgatat
    14521 ccgccttcat ttttcctctg gccccttgca gaatcgggta ggcctgtaaa tttttcagca
    14581 tatccaacgc ttcgcttttc gttaccggag cgagacgtat agacgtatcc ctgaagattt
    14641 ccagaaaaat tccgccgagt cccgcaatca caatcggccc gaagacgggg tcctgtcgtc
    14701 cgccgacaaa acattccacg ccgtcggcag acatttcctg aatcaggaaa ccatccatgg
    14761 ccggtcgcgg cttcatttcc tttagagatt ttttaatgtc ggaaatcgct tttttgagct
    14821 cccgctgatt tttgatattc aggcgcacgc cgccgatgtc ggatttgtgc gacgcgtcgc
    14881 gggaaagcag tttcacggca accgggaaat gaagagaaac gtctccggcc tctttcgcgg
    14941 atttgaccgt cgcgccggca atgcttttga tgcctgcggc ttcgactatg cgtagggatt
    15001 catcggttaa aggaattctt ttttgcgttt tgcatttgtc ttggatgata tcgatcgctg
    15061 ccatatcaag tgttgtctga acagctctgc ctctggcctc ccgcgccgtt ttttcctcgt
    15121 aataggtggc cgacatgttc agcgctttgg cggccgtcag tggcgtagta aaaatcggat
    15181 aactctgatt cttggaaata ttcaccagtt cctgcgtatc cgtgaccatg gtcatggcca
    15241 caggcttttg atattgctgt acaagtttgc cgacattgct caggaaatcc cgcgacattt
    15301 tcccctcata gtcggctatg tagaggtgat tgaacaaaac gccatccaca tcgggcagtt
    15361 tcagggcttc ttccagaatt tgggtaaaaa tggtgtaatc gaaaatttct cccagatcga
    15421 gcggattctg atgggcgatg acgcgcgtgt tgtagatggt tttcagattt tcaataaaag
    15481 ccggaggaaa atcaacaagc gaaaatccga atttcgcgca ggcatccacg ctcatcacgg
    15541 catgtccgcc ggatcgcgac aggatcgcaa cccgtctgct tttcatcaat ggcagccgga
    15601 taattttcac cgcgttgatg aaatcaatat catcgtcgac gcgaatgacc gccgtttgcc
    15661 ggaaagcgcc gtcgaccacc tgatcatcgg cggagagggc ggtggtatgg gactgggcga
    15721 ttttcgccgt gagatcactg cggttggatt tcagaacaat caggggcttg tcggattgga
    15781 ccgccagatc aaaaaaggct ctgccgcgct tgaatccttc cagatacatg gtgacaacgt
    15841 ccgttttgtc gtcattgagc agatattcca gaacatccac ttcatcgatc tgaagcttat
    15901 tgccgatggc ggcaaacttg ccggggatga tcgcgttatc gccgaattcg cccaggtagg
    15961 accctcccac gccgccgctt tgaacaatca tggccactcg cccgccgggc gctgtcctgg
    16021 gaaagaagcc gaaaggcatc atcattttga tatcgaaatt gatggtgccg atgcagttgg
    16081 ggccgatgaa acgcataccg tagcggtcgg cgatttccag cacctttttt tccaagacat
    16141 tttgtccttg cgagtattcg ctgaatccgc ccgattcaat cacaagccgc ttgatgccct
    16201 ttttaccgca ggcgtccatg aaatccggaa ctgtttcggc gggagcaatg atgatggcga
    16261 catccggcac ctccggaatt ttttcgacgc tgtcgtaaac aggcgcaccc gcgatatcgc
    16321 cttcttcacg gccgacgccg tagatgttcc cctcgtagtt gtgctgacgg ttgttcagaa
    16381 cgataatatg acccagatta agccgtttga cggatgcccc aatgaccacc atgctgcgcg
    16441 gataaaaaaa cttttccatt tttctgtgcg gttcctccct agttacacaa ttcggaatct
    16501 ccaagaatca tttgccgatt ttggatttaa tccttttttg atattccaca atttcatttc
    16561 ttaaaatatt cagatcggcg atgcggtcgt ccagtctgcg caactggtta ttcaaaagct
    16621 ccagcaggcg tccgagcacc ttgtcgttgg atttttcaat ggtccacatg gcctccagct
    16681 cctgcatctc cgataatgtc atgcccatga tcttcaggcg cttgatcagt ttcagacggc
    16741 ggaaatcggc ttcggtataa atccggcgtc ccccctcaac gcgcttgatg gaattgagca
    16801 ggccgatttc ttcataatag cggatagtgc gctggctcat atccagtttc ttagcgattt
    16861 ctccgataga ggtgaaggtt tcttgcgtgt cttgcatacg attcaccccc cgttaaattg
    16921 atgagctgag ctgttctttc agctcccgtt ttaaaatttt tcccgaagga ttcttgggca
    16981 gccggtcggt gatgaaaact ttcttcggac atttaaaacc ggccagacga tccttgcaga
    17041 atgagataat gtctttttcc tgaatcgaca cacccttttt caggacggcg accaccgcca
    17101 caacctctat ccatttggga tcgggcaggc cgataacggc gacctcttcg atgcccggat
    17161 actgataaag gacttcctcg acttcgcgcg aagcgacgtt ttctccgccc gttttgatca
    17221 tgtctttttt gcggtcgacg acatagagat agccgtcgtt gtcaaatcgt cccagatcgc
    17281 cgctgtggaa ccagccgaac tggaaggctt ccgctgtttt ctccggatca ttcagataac
    17341 cggacatgac atgaccggag cgatgaacaa tttcccccac gtcgcccagt gcaacaaatt
    17401 ttccgtcatc atccatcagc cgggtttcca catttaaaac gggtttgccg gcggaaccgg
    17461 gcttcaggag ctgatcttcg ggcttcagga tggttgccac cggccccatc tccgtctgcc
    17521 catagtaatt ccagagcttc aagcctttaa atgtgacgga caactgtttg atgatttcca
    17581 ccggcatgat gctggccccg taggcggctt ttttcaggct gccgtggttg tagttgttga
    17641 attccggatg attcaggatg gcgatccaga cggtgggcgg agcaaacatg tgtgtaatct
    17701 gatatttttc gatcagccgg atcatctcca caggatcggc cttatgcatg atcacgttgg
    17761 ttgcgccgac atacagataa ggcatcagga aacaatggag ctgagcgcaa tgaaacaggg
    17821 gcagggcgtg gagactgacg tcgtcagtct cataccggcc gtcaaaaatg cagctgtgat
    17881 actgcgacat caatgccctg tgagacagca tggcgccttt gggtttggcc tccgtcccgc
    17941 tggtgtaggg aatctgcacg atatcttctg cgctgacctc cacctgcggt tcgtcataat
    18001 tcatcgccga catctcggcg attaaattga agaacccgtc cggaatgcca gaatcattca
    18061 gcggaatgaa tccccatttt tccacggcgg ggaatttatc cttgcatccg gcgatgaccg
    18121 gatagagcgt atcttcgatg atgaataaac gggaaccgga atgattgatg atgaaggcaa
    18181 tttcttccgc gttgagcatg taattgatgg gaacctggat ggcgccgatt ttggccagtc
    18241 ccatcatgct gatcggataa taatgggagt tataggcgta aacggcgacc cggtctccct
    18301 tccggacacc gtagcgggtc atcagattgg caaagcgccg cgcctcccgt tccagatcgt
    18361 agaatgaaag cttgatattg cggaaaataa tggcggtttt atcgccgtat tttgtcgccg
    18421 ccctcctcgc catgtcgccg atagtatcgc ggttgatgat gttgctcatt ttcttacctt
    18481 tctccctgat agtttttaag caggatattt tttttcaaac acttccttca tcttgaattt
    18541 ctgaatcttt ccgctggccg ttgtcggata gtcgtcaaca aactcccagt atttgggaat
    18601 tttgtgccgg gcgatctttc cctgacaaaa ctcggtgaat tcttcaggcg tggccgtctg
    18661 tccggccttg agttgaatgg cggccaggac ctgttctccg tattttttat ccgggatgcc
    18721 gatgacctga acgtttacga ctttgggatg cgtgaaaaga aattcctcaa gctcacgcgg
    18781 ataaatattt tctccgccgc ggataatcat atccttaata cggccggtca ctttgaaata
    18841 accgttatcg tccatctctc cgagatcgcc tgtgtgtaac cagttgtatc ggtcgatggc
    18901 gtcagccgtg gcatcgggca ttttgtagta gcccttcatg acacattcgc tgcgggtgac
    18961 aatttcgccc tgcgcattgg gaggcaaatc aatgccgaga tcggggtcaa caatctttcc
    19021 ctcgatatcc ggcagaagcc gtcccacggt ggagacgcgc aattccacag gatcgtcgcg
    19081 ccgggtcatg gtcatgaccg gcgccgattc ggtctggccg aaggcgatga caatttcggg
    19141 gcaatgcatt ttcgtgcgca cctggttcat ggtttcgaca ggacagggag cgcccgccat
    19201 gatgcccgtc cgcagagaag acatatcata tttgtcgaaa tcggggtgat tcagaatcgc
    19261 gatgaacatg gtcggcacgc cgtttacggc cgtgcagtgt tctgcggaaa tccattgaac
    19321 agctttgagg ggatcgaaat attccacgac gaccatggtg gagccgtgat aaacgcaatt
    19381 aagcgaactc attacacagc cgaaacagtg aaacagcggt acctggatca gcagcttgtc
    19441 tttttccgtc atgcccatga cgtcgccgac catgcgtgca ttgttcacga tgttatgatg
    19501 cgtcagcatg acacccttgg ggaatccggt tgtgcccgag gtgtactgca tattgatgac
    19561 atcgtcatca tggaggctgt ggagcctatc ctccagctgt ttatcggtca cctccctgcc
    19621 catggtgatg atgtcgctga aatgcagcat gcccggggtg ctttcccgct tgccgatgta
    19681 gataatattt ttcagagcgg ggagttgtgc cgaaaccggg gttcccggct ggtggtgatc
    19741 cagttcgggc aatattttgc ggacgttttc gtaaaaatta acttctctat aggactccat
    19801 taaaaaaagc gtcgtggaat ccgattgctt gagaatgtat tccagttcgt gcgtctgata
    19861 atttgtattg acggttacca gaatgccgcc cgtcattccc agcgcaaatt gcagatgaat
    19921 ccagtcggga atattggttg tccagacggc gacattatcc ccgcgtttga ttcccagggc
    19981 gataagccct ttggcgacgt cccggcacgt ctggtaaaat tcactgtacg tctgacgaat
    20041 gccgaaatcc ggacagacaa gcgccgggtt ttgcgggtag cactcggctg tttccctgac
    20101 aagttggccg attgttttct tcacaaaatt tgacatttaa tacctcctcg aagacgtttg
    20161 atgacttttg tatgatcaaa ctacttcatg tcctttacgc aaacagacta tcggacataa
    20221 tactataata ttacgatcgt tttttatgtt aagtccagct tacgttaact ggaatgatat
    20281 gaccggcaga tgattttgtc aaggcaattt tatagttttt tatacggtaa gaaaatcttt
    20341 tattttttaa tcggcagccg gcggacacgc ttttgctgta cagattcaag agcagatctt
    20401 cggacaggac gtttggattg aaattgagtt tgctggtttg tttcccgttg cagacttttt
    20461 ggggtgtaac gtaagaattt atcctcgcag acaatttcct tgacacagac aggcatgtta
    20521 cctatctttt agccgcaaaa aataaatatt ttcattttat gctgcggttt tttattgctg
    20581 ttttcagttc agaaaggaca atgctgtgat accaaaaata atccatcaga cctggaaatc
    20641 aaccgagatc cctccgcgct ggcaaagatt cagggacgca tggaaaaaat ttcatccggg
    20701 atgggaattc aggctttgga cggacgaaga caatcgtcgt ttggtggaaa gagattatac
    20761 ggcttttctg caactttacg actcctatgc gtatccggtt caaaaagcgg attttgccag
    20821 aattttatac ctgcacaaat ttggcggtgt ttatgccgat ctggatctcg aaccgctccg
    20881 ccccctggac gggatcatcg atcaacctca tctgattctg ggggaagaaa aagaagggat
    20941 ggggaagtcc ctcaacaggt gtgacttcat cattaatgcc tttatggcct cgccgaaggg
    21001 gcatccgttc tgggaaatgc tgcttgagcg ccttcgggtg acattccgtc acaaaaggct
    21061 attgcaatcc aaaggccatt acatcataca gaccgtcatt tttgagctgg acaaactggc
    21121 cagagactat cagcaaacgc atcaggacat cacgatttat ccgtatgagg tgttttatcc
    21181 gctgtcctgg cacgaagcgt gttccgaatt ggaacgaagc aaggcagttg aaatgtccta
    21241 tacgattcat cattttgaca caacctggtt ttcaggcggt atgatgctga tgaaaaaact
    21301 tcgccgccgt ctgaacatct gacggcatat tttttatgca caggctgtgg ttttaaaata
    21361 cgatgatgta acgaaaacaa tctgacggac gaaagagacg gttatggaaa aaactcttct
    21421 gataattttt ttaattttgt ttgtcggccg gatcctctgg cgatttattt tgcagcaact
    21481 caacatccgg catttgcgcc agtgcggaaa aattattccg ccggctttcc ggaatgtgat
    21541 tgatcaggca acgctttcca aaatggcgga ttatacgtat gataatttgc gtctggagaa
    21601 caaggaaaat tccacagaag accttctgga acttgccatc atttttcttt tcctaccgct
    21661 gctggttgca aaaacaaacg gattgcagct tcacttgatc tggcaggcgc tggttttctt
    21721 cggaactctg gcgttgattg gcggcgttat cggacttccc tttgacattt accgcacgtt
    21781 tgttctggaa aaaaaatacg gcttttccac gattacatgg aagctctggc tgaccgatct
    21841 cgtcaagtcc gtgatcattt cagcaattct gatggggttt ttactttccg ttttcatggc
    21901 ttttgttctt tacctgccga caagctggtg gttctgggca tggatatttt ttgccgcgtt
    21961 cgagatcctg cttttgtggc tttttcccgt ggtcatcgcg ccgctgttta acaagtacga
    22021 accggtcaag gatgaagaac tgaaagagaa aatcacatca ctactttcca aagtcggatt
    22081 gaaggccaaa ggcatttttc aggttgatga aggtaagcgt tccaaacata ccaacgccta
    22141 ttttacaggt ataggcaaaa ccaagcgcat cgtacttttc gacacgctgc ttgcttcaca
    22201 tacgcatgat gaaatactgg cggtgcttgc ccatgagatc ggtcactgga aaaagaaaca
    22261 tatcctgaag caattaatat ttatggttgc ggcttccttt gtcggatttt atctggttta
    22321 tctgctggta aactggccgc cgatctatgg cgccttcggt ttaccgcaaa cgcctgttta
    22381 tgccggtttg cttctggtgt cactgtattt cagttgcgtc ggattcttct tcagccctct
    22441 ggccgcgttc atctcgcgcc gatatgagcg cgaagccgac aggatggcac atgaattgac
    22501 cggaacgtca gagccgatga tacatgccct caaacgtcta gccaaagata acctgtccaa
    22561 tcttcatccc catccgtggt atgtgtggtt ttattattcc catccgccgc ttattgaacg
    22621 aatagaatat ctgaagacaa tggacaaagg gaatctgcag cgccaacgat aaaaaacatt
    22681 atgatgtcct gttcatacac gattcatgga tggcgcgcat aacggccaac ggatcggatg
    22741 catcgtttac ggcgcggatg actgccgtat tgtgcgggcg cggttgtaat tgcctgatac
    22801 gctccagggt cagcccgccg atagccacga tgggataacg gctgatggcc agcgcggcct
    22861 tcaagatatc cggtcccgtc accggtgcca gccgttcctt ggaattggtg gcaaacagcg
    22921 gcccgaaccc gatgtaatcc acgggtacgg catttgcggc cgcgacctgc tctaggttgt
    22981 gtgtggaaag acccagcagc atggtcggac cgatgagctg ccgcacctct gctgccggaa
    23041 ggtcatcctg tcccaaatgc acaccgtcgg cttgcgaaat aagtgcgatg tccggccggt
    23101 cattgacgat gaagagcgtg ccggtacctt tggtgatttg tcgcagggca cgtgccagag
    23161 caagatgttc tcgttcatta tgacctttat agcgcaactg aacggccgga agaccggctt
    23221 ccacagccca ccggcaaagc gtttcataac cgtcgcgcgg atcggtgagg atcaggtaaa
    23281 gcccgtgccg caggaatgtg cgcattattt gtccctctgc cgtattcata agcgcgctag
    23341 aatttcttca gccaccttga gtcccgagag cagcatgccg ccgaagaccg gacccatgcg
    23401 gtagccgcca tagaccgcgt tagcggccat gccgctgaca aaaagtcccg gataaacctc
    23461 gcgggtattt tcgacggttt gcaactctcc ctttccggct tccatggatt tttcgcccac
    23521 gatcccgccg gtgcttgtgt tgagtctcac gcccattttc cgtacgagca cctcggtgac
    23581 attggcggga tggcccgtcc cgtcgataac atatcgggca tggacggtta gcgggtccac
    23641 atgccatttt agcgtatcaa ccgcgcccca attaatgacc agaccggcca cgcgttcgtt
    23701 tttgattacc acatcctcta tggcgatgag gttgaagacc tgcaaaccgg cccgcacagc
    23761 cttgtgaata agcgttgacg tgatatccac ggcatcaagg acataatagc ccggcgtata
    23821 ttcccggccc tggaggtcga attcttcaag caattgccgt ccggcctcct gtaccacgat
    23881 ggaattgaac atcatgccgc ctccccaaat gcctccaccg attgagagct tgcgttcgaa
    23941 aagagctacc ttccgtcccg ctctggccag atagtatccg gccaccagcc ccgacgggcc
    24001 gccaccgacg atggcaacat caagatccag acagctgccc agctttttaa aataggaatc
    24061 cagaattgcc cgactgataa tgatttcgtt taacatgact acctcccaaa gattaacctg
    24121 ggcggcttgc cggctggata tccaaaaaaa atccgtttcc tcatcagggg aaacggatca
    24181 atacgccttt tttcttccta cgccggcatt acccggatca ggttcaaagg gtataatctc
    24241 agaccgttaa ggccacccct tttagttgcc tatccgatgg gaaagagttt gtcaagaggc
    24301 tgatgagcat tgatactgac tttttaaaat tctttgagaa aagcgggaag gcctttgatg
    24361 ctgtccagga ccttgcaggc tcctgcgaaa tcgtggttgc acgtgaactt gctggggatg
    24421 acaatgcacc ggagtccggc gcttgtggcg gcggccagac cgcgcggcga atcctcaatg
    24481 accagacatt cctccggcgc aaaaccgctt aacgcaattg ccttgagata cggtcccgga
    24541 tgcggcttgg tataagggca atctttcagg gtcaggaaaa actggaaata gggcagaaga
    24601 ctcgattgcc ggtgaatgat ttcaaagtgc tcatgtccgg aactcgtgac gatacccatc
    24661 aaatattttc cctgaagcgc atccagcact tcccggacgc cgtcaatcac cagcgggtcg
    24721 cgggtcagca aatcggcata aagggcgtca cgatcccggc gaagtttgat aaacgcatcc
    24781 gtccccgggg ttgccgccaa ccgcatcacg ctttctcccc tgacaagcga tatttcttca
    24841 aattgctccc gttgcagggg aattcccaaa cctgccagga tttcctgatt ggccctgaaa
    24901 aaaaggcctt ccgtatcgac cagaacaccg tcattgtccc agaaaattgt cttgatcatt
    24961 aaac
//
LOCUS       DAONQZ010000031        24256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2305_length_24256_cov_18.456221, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000031 DAONQZ010000000
VERSION     DAONQZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24256
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_24256_cov_18.456221"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLR13_06675"
     CDS             complement(<1..483)
                     /locus_tag="PLR13_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19058.1"
                     /translation="MRKNIITKILLSRKLYQLSLENLNSDNELSLSIGVILLQDSVEI
                     FLLAVAEHIDASIGNNSNFNQYFDLINKKLSPKELPFKLRLTALNKLRVNAKHYGLSP
                     SKLETHGLIETVKEFFEEVSIQVFEKEFVTISLVDLINKGEAKDYLMAAENDFSKGDY
                     V"
     gene            complement(670..2832)
                     /locus_tag="PLR13_06680"
     CDS             complement(670..2832)
                     /locus_tag="PLR13_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC19059.1"
                     /translation="MSENPLHLLMNPQSIAVAGANNNPSKMGTIQALNIVKGGYGGRF
                     YPIHPVEKTVLGCPAYATPEVLPEAPDLAILIVPIRAVASLLEGFGKIGTKRAIVITA
                     GFKETGAAGRDMEKKINEIADRYQMRFVGPNCMGVINSGISLNTTVLATSREPGLLGF
                     ASQSGTFLSQTLPYLNKRGIRFSKAISLGNEANINMIDALEYLGEDEQTKAIILYIEG
                     IREGRRFLDVARNITPHKPIAALYVGGSASGARAGLSHTGAMAGPDFLYNGIFKQAGI
                     IRVNTIEDLYYHGWTLATQPPMRGKRVGVMTNSGGPSTTISYTCDAVGLEVPRFSDGL
                     QNEIRKHIEPHASASNPVDMTFDLSMNKLALTLPEMVMKSGEVDAVVLHGTMMTGYLK
                     EVYPTLKDIIGNISLEDFLKYGQMDRTIANETFKLPSKYNMPMLISSFFDHEDNYIKG
                     YQDTNTPVFYSPENTARALGSLYLYKQIKERAPRKEAALPKIKKEATEIILKALDNKQ
                     KALDEYEAKQLLACYGVPVTKEALAAKVEDALKTAKKIGYPVALKACSWKIMHKSGKG
                     LIALNVENENQLKKEFENIQKNAGKTAPVLVQEMLSGSREFLAGMTRFAGFGPGVVFG
                     LGGVFTEVFKDTTMRLAPVTSADALEMFEDINARKLLDKFRGLPKVKLSNLSRIIQAL
                     GNIALLHPQIAEIDLNPIIINGAEPVVADALIVLEDKS"
     gene            complement(2832..3434)
                     /locus_tag="PLR13_06685"
     CDS             complement(2832..3434)
                     /locus_tag="PLR13_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC19060.1"
                     /translation="MPPKVIYSRSDIIDSSLRIVKEKGFKQLSAREVARQLISSTRPV
                     YEHFQSMDELKKAVLQKTVDLLYDYVTRQYTKNAFLNTGVGYVLYARDHKEFFKMIFL
                     EDNDASGIIDEMLKKLDKEIVKVPELKKLAPAERKDLLKNGWIYTHGCAVMVFAGYIK
                     NNQDKQIIKMLAEAGPIFIEDALRKHNADALINTTIKEIE"
     gene            3692..5173
                     /gene="lysS"
                     /locus_tag="PLR13_06690"
     CDS             3692..5173
                     /gene="lysS"
                     /locus_tag="PLR13_06690"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQC19061.1"
                     /translation="MEDNELLNIRREKIADLKSAGIDLYPNDIKPKNTTQEITLKFGD
                     MDNDSLAKQEETFSVAGRLMAVRNFGKAAFIKIQDYKGQIQGYIGKNSLSPEEYFVFK
                     KLDIGDIIFISGKLFRTKTNELTIEAQKLMLLSKSIRPLPEKWHGLTDIETRYRQRYL
                     DLIANPKVKEIFHRRSLIIKLIRQFMDEHDFLEVETPMMQPRAGGAAARPFKTHHNAL
                     DMDFYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYQSYATYE
                     DLMAFTEDLFCFLAKNIFGGLKFNYQDTEIDLTPPWRRISVKDALLQLANMDAAALDD
                     PALALSFARKVGCKVNDNDPLGKILMAIFDEVVEKKLIQPTFVTHYPVVISPLSRRSK
                     NDPDLTDRFELYIYGREIANAFSELNDPVDQKGRFLQQLHEREAGDDEAHEMDEDYVR
                     ALEYAMPPTAGEGIGIDRLVMLFTDSSSIRDVILFPLLRTRQE"
     gene            5235..6518
                     /locus_tag="PLR13_06695"
     CDS             5235..6518
                     /locus_tag="PLR13_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQC19062.1"
                     /translation="MPYELFISRRYLKAKRKQVFVSVITFISVLGIFLGVAALIIVLA
                     VMNGFEEDLRTKILGIKSHVELTTDMSGPMKNYRDVRKKIAGIEGVVASTPFIYTQAM
                     IRNDNGVTGVVIRALDTESAFRVINLGKIKEGNLDHLNKIPQDIAAGYSDNGVQPDGI
                     VIGKEMARNMGIFLYDTITIISPVSIATPAGMVPRMGKFVVVGIFESGFYEYDSTLAY
                     LSLPSCQKFLQMGDTVTGIDIKVNDIYKADVIARKIQSALGFPYWTQNWMQMNKNLFS
                     ALKLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKDIAILKSMGATSASIMKIFVY
                     QGLIIGVIGTFLGCAAGLAVALNLQKVSLFVEKIFNFKFLPGDVYYLSELPSKVNYSD
                     VAIIVAGTLLICFLSTIYPSLRASKTDPAEALRYE"
     gene            6543..7214
                     /locus_tag="PLR13_06700"
     CDS             6543..7214
                     /locus_tag="PLR13_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19063.1"
                     /translation="MIVLENLSKTFFKAGNKIEVLRGLNFKIEKGQSLAVLGASGAGK
                     STLIHIMGMLDHPTGGLLLLDGRNVFAWEEKKRAAFRNSHIGFVFQFNNLLPEFNALE
                     NTMMPALIGGFNKKQAGEKACKLLQEVGLESRITHKPGELSGGEQQRVAIARALIMEP
                     DILLVDEPTGNLDTETGKKIEDILVNLNVTKKITLVTVTHNKLLAERMSERILLRDGE
                     IYEYQ"
     gene            7201..9846
                     /gene="bamA"
                     /locus_tag="PLR13_06705"
     CDS             7201..9846
                     /gene="bamA"
                     /locus_tag="PLR13_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQC19064.1"
                     /translation="MNTSKKSFKISLALLLFVFLFVPPAYSEDLKKIVVMPFEVFSNQ
                     DKTVIRESFYKNLQAELQKEKSIQIIAADDFLQREGQIDENTALDHGKAKGADFIVTG
                     TLTQFGKSMNIDCKIFDVEKQSSLPAVSTHGRGMASTTALAEQLKVEILDRIGIVQKI
                     ARIEIQGNRKINASAITTKLKSRKGGIFSEADIAADIKTIFKMGYFLDVTAETDATPE
                     GKVITFFVQEKGLVSEIVINGNKAVSKDDIQEVLTIKTRQSLNREQIKADIEKIKALY
                     DSKGYYNAEIKDRVEQDGETDFRVVLEIRENDRLYIRSIAFEGNEAFPAKELKKLMST
                     SEKGFFSFITDSGLLKRDQLKQDIGKINAYYFNNGFINAQIGEPETTADQKGIHVKIK
                     IQEGKRFKVDNVAISGDVLEKPREALLQSLKVKKGDNYNREAIIRDLDLLMQACNDEG
                     YANAEINPKIDTRENEQLVDVDFQIAKGEPVYISNINISGNSITRDKVIRRQLDVVEG
                     DLYSSSKLRSSYANLSRLRYFEEVDFQTEKGTDKNKMDINVRVKEKSTGMFMVGAGYS
                     ASDKLVMMAQITQQNFLGYGQVLSLKASLGSETSNYDLSFTEPWLFDLPLWCKADIWK
                     YEKEYDYYTIDTQGAGLTLGYPLIERIVGYLGYKYYIDDIREVSPIATDYVKAQEGET
                     SSSAVTLSLIRDTTNDYIFPSKGTKATVSVTHAGGPLEGDANYTQYGASLFAYFPLPL
                     DVVFAAKGRIGYIQDHEGVEIPVSNRYVLGGINSLRGFRYVGPTNGGTSDVIGGTNMM
                     VFNVELVLPFIKESGMKLVAFYDAGNSWDNKYDMGDLRQSVGAGIRWYSPIGPLRLEY
                     GHVLDRGELNDDSKGRWEFTIGMPM"
     gene            9895..10413
                     /locus_tag="PLR13_06710"
     CDS             9895..10413
                     /locus_tag="PLR13_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQC19065.1"
                     /translation="MKKFFYLTAIMTMLVFAWSANVLAADKIGFINLQEIMQTSNAGK
                     KAADEFKRFYEKETQEIKSMENQLKKMKDELEKQGSVLTQSSRSEKEAAYQKKMRDYQ
                     LLVNDTNEELKKRDQEMTQKLMPGIIKIVRSIAEREKYTLVIDVATMPIPYYAKENDF
                     SNKVIEEFNKTK"
     gene            10438..11454
                     /gene="lpxD"
                     /locus_tag="PLR13_06715"
     CDS             10438..11454
                     /gene="lpxD"
                     /locus_tag="PLR13_06715"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQC19066.1"
                     /translation="MTTGEIAAFLGGTVMGADDVVIGHIRAIDEAGPGDLTFIANKKY
                     IKKLKTTKASAILVSPQTTAEGKNLIVVSDPYVALGKILSHFYPAQHGRSGVSAEACI
                     EDGADVSPDAVIFPRAFISAGAKIGAGTVIYPGVFIGRNASIGEHSIIHANVTVYHSC
                     IIGNRVILHSGAVIGADGFGFAAPGRDNVKIPQVGFVQIDDDVEVGANTTIDRATLGR
                     TWIQRNVKIDNLVQIAHNVVIGENSVITAQVGISGSTQLGKGVMVGGQTGMVGHITIG
                     DHVMIAAGAGIHKDIKSGDMVGGAPQLPYKEWLQVEACRAKLPEMRATLNDLVKQVEE
                     LKKK"
     gene            11478..12251
                     /gene="lpxA"
                     /locus_tag="PLR13_06720"
     CDS             11478..12251
                     /gene="lpxA"
                     /locus_tag="PLR13_06720"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQC19067.1"
                     /translation="MKIHETAIIAPGAQLEEGVEIGPYSIIGADVRIGKNTVIGPHTV
                     IDDYTQIGESCRIFQFCSIGAPPQDLKFSGEKTRVVIGNFNTIREFVTIHRSTTADIG
                     VTIMGNHNLIMAYCHVAHNCKLGNHVIMSNAATLAGHVHVEDYAIISGLTGVHQFCRV
                     GAHCMIGGCSAAVKDIPPYTIAQGNHAKLYGLNLIGLKRRNFPEKTIKAISDAYRIVF
                     RSKLLLEDAIKKAESEVEDLPEVRHFIKFIKESERGICR"
     gene            12262..13215
                     /locus_tag="PLR13_06725"
     CDS             12262..13215
                     /locus_tag="PLR13_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQC19068.1"
                     /translation="MKHKKIKVGVIGIGHLGNYHLQKYRKLEPCEIVAVADTSSERAQ
                     KAAQIYQCAAFSDYREMIGSVDAVSIAVPTSDHYNVAREFLAAGVDVLIEKPLCATLA
                     EADDLIGLARKKKLILQVGFVERFNPAIMALENVIRKPLFIESHRLHPFFERGTDVDI
                     ILDLMIHDLDILVKFVDAPAISVEAMGVPILSDKIDIANARITFSTGCTANITASRIS
                     AKTMQKIRFFSPEGYHSVDCQKREVLSFSKGTTGSGQMQIVQNHLEIGSHDPLEEEIR
                     SFVDAVITRGCPPVSGEEARKSLALAVDIITKMKAAEDLLT"
     gene            13221..14306
                     /locus_tag="PLR13_06730"
     CDS             13221..14306
                     /locus_tag="PLR13_06730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQC19069.1"
                     /translation="MVDLKRQYHSLKRDIDAAISNVLEDTRFILGPNVSALEEEVAAY
                     HGPSQAVGVANGTDALLLALRASGIEAGDEVITTPFTFIATAEVVALLKARPVFVDIE
                     PVTFNMDWRQIEGKITSRTKAIIPVHLFGHPADMDPIMAIAKKHNLRVIEDCAQAFGA
                     VYHGRKVGAIGDIGCFSFFPSKNLAGYGDGGMIVTRSDETAAKIKMLRNHGSARRYYH
                     DEVGYNSRLDEIQAAIIRIKLKKIDEFNAARRRNAALYCAAIKRKDIVLPSASGDCEH
                     VYHQFTIRSDERDLLADALQKKEVASAVYYPVPLHRQNAFLNLYDGPETMPQSETCAR
                     EVLSLPMFPELDQKEIEFIADIINKAS"
     gene            14327..15475
                     /gene="lpxB"
                     /locus_tag="PLR13_06735"
     CDS             14327..15475
                     /gene="lpxB"
                     /locus_tag="PLR13_06735"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQC19070.1"
                     /translation="MKDSNHKNKTVLIIAGEASGDMHGANLVREMLNRDPALKFYGIG
                     GKKMLQEGVQLLADASTMAVVGLTEVVFKLKDILKIMRTIKKSLDERRPDLVILIDYP
                     DFNLPVAKAARRHNIKVLYYISPQVWAWRQSRIEQIKKTVTRMAVILPFEVETYRRHG
                     FAADYVGHPLLDMIKTTYSKKESRKKFDLHEDKITIGILPGSRMSEIKKLLPELLQAA
                     TIMKDSIPELQFVVPLADTLEEKNLTGIIAGFPIDVNVISGQTYDVVSCADLALVASG
                     TATLETALLKVPMIIVYKISPLSYFIGKLFVRVKNIGLVNIIAEKTIVPELIQNDASG
                     ARIAREALSILGDETKKQAMIRELEAIRSRLGEPGAAIKTAKLALDML"
     gene            15492..17255
                     /gene="msbA"
                     /locus_tag="PLR13_06740"
     CDS             15492..17255
                     /gene="msbA"
                     /locus_tag="PLR13_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HQC19071.1"
                     /translation="MKIFKRLLSLGRPYSVRFLISMLCMVIAGALTSSQAVLVKPVLD
                     DIFLAKNPASLKWLPLAVVMIFLVKGICNYVQTVLMNFIGLRIVTDLRNQLYEQIQKQ
                     SLSFFTHHPTGLLMSRITNDVASIQNAVSDVVTSIVKDSFTIVALICVIFYTDWKLAF
                     IAMIIFPLTIYPISAFGKKIRKVTTSTQITMANLSSLLQETIAGTRIVKAFGMETHEI
                     KRFAGENERFFKLNMKAVSVKALSHPLMEFLGGLGIAAVIFYGGYNVLAGNSTPGTFF
                     SFLTAVLMLYEPVKRLTNVNNTINQGIAGAERVFSIIDLVPDIQDKPHAAELPPIDRG
                     IEIDNVTFRYEKTPVLKNITLSIRAGEVVAFVGMSGGGKTSLVNLIPRFYDVSEGRVL
                     IDGLDIRDVSLPSLRRQIAIVTQQTILFNDTVRNNIAYGSFEKSDDEIICAAKAANAH
                     DFIMRMPKGYDTNIGELGTKISGGEKQRLSIARAILKNAPILILDEATSSLDTEAEME
                     VQEALDNLMKGKTTLIIAHRLSTIRNADRIIVLVNGEIVEEGNHETLMQKQGEYFRLY
                     NLQFKDEGSHENNNRLAKDLG"
     gene            17218..18330
                     /gene="lpxK"
                     /locus_tag="PLR13_06745"
     CDS             17218..18330
                     /gene="lpxK"
                     /locus_tag="PLR13_06745"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQC19072.1"
                     /translation="MKTITDWPRIWDNDGSASGYGTIKFIAHLLSFFYLSIVNLRNWL
                     YDHNLLKATRLGCPVISVGNITVGGTGKTPCVVMLARMLQHRGFKPAVISRGYGGRGK
                     GAVNIVSDGQRILLDSEIAGDEPLLMAHALTGIPVITGPRRTATGKTAIDHFGADVLI
                     CDDAMQHRQIFRDVNLVLIDGQDLKDNIHVLPRGRLREPVKALRRASAVVFTRVDGQI
                     AVHQTIENLITKENLPVFQSLHRINDMISADKKICKPFTELEGKRVHAFCGIANPESF
                     KKTLLSTKAVLVAFNIFPDHHRFQEHELEKIKNDFKNSAADYLITTEKDAMRLKNHPE
                     MSKMLFVLRITMEIKDNPQSFENFILHKIRAGTKKG"
     gene            18341..19408
                     /gene="waaF"
                     /locus_tag="PLR13_06750"
     CDS             18341..19408
                     /gene="waaF"
                     /locus_tag="PLR13_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HQC19073.1"
                     /translation="MKDEKIKKLPQKGIHKILIRGTNWIGDAILTLPAVASIRATYPQ
                     ARIAMLVKPWVADVYDIFSNLDEVIVYENKYDTATGVFGLARMLREKQFDAAILLQNA
                     IEAAIIALFARIPVRAGYNSDARGLLLTHSVPLTRDIKKVHQVNYYLEMVKALGCVSA
                     NRDMNLETKISREEAQNVLRRYVAPSQKMLIGIAPGATYGAAKKWFPDRFAETLNRLD
                     EQFSVQGIILGGKADRITAEEVRTSARADMINLAGETSLREAIYLISQCRLFISNDSG
                     LMHVAGALNVPTIAIFGSTNPTTTSPVGKQSLIVRKEVSCSPCLKKTCPTDFRCMNLI
                     TVEDVLSVAQTLLKDNRDKDQ"
     gene            19405..19992
                     /locus_tag="PLR13_06755"
     CDS             19405..19992
                     /locus_tag="PLR13_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQC19074.1"
                     /translation="MKKNTAVFLDRDGVINEEVGYLDGLEKLKVIPNACKAIQMINQS
                     GMKAIVISNQSGVARGLFTEEFVQTANSHLQKILKTHGASIDDFYYCPHHPSEGKAPY
                     RQICDCRKPAPGMLLRAARDLNINLTLSYLVGDRYIDMIAARRAGVKGILVKTGYGND
                     LLQDDGPDRPTPESEPDFIAADILEAVQWILKDKT"
     gene            19968..21032
                     /gene="waaF"
                     /locus_tag="PLR13_06760"
     CDS             19968..21032
                     /gene="waaF"
                     /locus_tag="PLR13_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HQC19075.1"
                     /translation="MDIEGQNVNILIVKLSAIGDVIHTLPSLAELRRLYPDAHITWVV
                     EEAAADLVKHHPCLDEVLISRRKTWIQNVRAGKIRQSLNEGISFIKGLRKRHYDLVID
                     FHGLFKSAIIVFLSRGRRKLGYDSWQELSGLFLNEKIPEDMDKHAVDRYLDFLRHLGA
                     KTPEARFVLPSNDEEEKKAQLLMQKHRLEDKKFIAVNPVALWDTKLWSNEKFARLADL
                     IQNRLKLNVVFTGSDKKPLEAITALMTTAGLHLGGETTLICLAGIYKKARAVVTTDSG
                     PMHLAAAVGTPVIAVFGPTDPARTGPYGSRHTVIRSGEDCSPCFLKTCPEKKCMDHIT
                     PEQVFAAVEKTVMEEKNGVK"
     gene            21019..21189
                     /locus_tag="PLR13_06765"
     CDS             21019..21189
                     /locus_tag="PLR13_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQC19076.1"
                     /translation="MALNKKLLEILICPKCRGKVRFDEKANGIICDKCKLLYEIKDDI
                     PVMLVEEAKNID"
     gene            21481..22728
                     /gene="rho"
                     /locus_tag="PLR13_06770"
     CDS             21481..22728
                     /gene="rho"
                     /locus_tag="PLR13_06770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQC19077.1"
                     /translation="MNIEDIKRLPISELNELGKKLDVPGASGMRRQELIFAILQTQAE
                     QNGVISGSGVLEILPDGFGFLRAIDYNYLPSPDDIYISPSQIRRFNLKTGDTVSGEVR
                     PPKEGEKYFALLKVDTVNFESPEQARDKILFDNLTPLYPQEKLNLEYDPKNYSTRTLD
                     LFAPIGKGQRGLIVSPPRTGKTVLLQDIAHSIAANHPEVVLMVLLIDERPEEVTDMQR
                     SVSGEVISSTFDEPATCHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRISDRRIFPAFDLIRSGTRKEELLIPKNNLNRVWILRRLLQEMNPVDA
                     MEFIVGKIKKTENNQEFLDSMNS"
     gene            22864..23097
                     /gene="rpmE"
                     /locus_tag="PLR13_06775"
     CDS             22864..23097
                     /gene="rpmE"
                     /locus_tag="PLR13_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQC19078.1"
                     /translation="MKEGIHPDYQETTITCVCGNVIHTRSTKKDIKTEICSNCHPFMT
                     GKQKIVDTAGRVERFKKKYETKVDATTSKKKKS"
     gene            23376..>24256
                     /gene="prfA"
                     /locus_tag="PLR13_06780"
     CDS             23376..>24256
                     /gene="prfA"
                     /locus_tag="PLR13_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQC19079.1"
                     /translation="MDIIRDKLHDIELRYRELEGQLSDPKIVSKPGMYQKYAKEYADL
                     KELVETIREYNTLRKQIDEYQDVMTEGDEELKAIVKEELPQLKQQQTDLEDRLTILLL
                     PKDPNDNKNVFLEIRAGTGGDEAGLFAADLFRMYARYAEAQGWRVEVVSSSPAGGMGG
                     YKEIIAQIEGDGAYSRLKYESGVHRVQRVPVTEAQGRIHTSAVTVAIMPEAEEVEINI
                     DPNDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKNKAMKVLR
                     ARLLDAEVQRQNAEQAQT"
BASE COUNT         6615 a         5904 c         6010 g         5727 t
ORIGIN      
        1 aacataatct ccctttgaga aatcattttc agcagccata agataatctt ttgcctcacc
       61 tttattgatc aaatcaacaa ggctgatggt tacaaattct ttctcgaata cttggataga
      121 aacttcttcg aagaactcct ttacagtttc tataagacca tgtgtttcta atttagaagg
      181 agataaacca tagtgcttag cgttaaccct gagcttgtta agagcggtta ggcgaagctt
      241 aaagggaagt tcttttggag aaagtttttt atttattagg tcgaaatatt gattaaaatt
      301 tgaattatta ccaatactag catctatatg ttcggcaaca gcaagaagaa atatttcaac
      361 agaatcttgt aatagaataa cgccgatgga aagagaaagt tcattatcag aatttaaatt
      421 ttcaagtgat aattgataaa gtttccgaga gagaagtatt tttgtaatta tatttttccg
      481 cattatttac tccttaacgg atttttataa caattcccta tctttcttat gacactctta
      541 atgcaaaagg attgcttaat aattacggat tgtaacctag cataaaatta tatttttgaa
      601 aaatgaggat ttcaggaagc aagacttgac actaaattat taggccaagt ctcctgcgga
      661 gcgataaact taagatttgt cttccagtac gattaaggca tcggcgacca ccggttcggc
      721 gccgttgatg ataatcggat tgagatcgat ctcggcgatt tggggatgca gaagagcgat
      781 gttgcccagc gcctgaatga tcctggacaa attgcttaat ttcaccttcg gcaggccgcg
      841 gaatttatcg agcagtttac gggcattgat gtcttcgaac atttccagcg catcggcgct
      901 tgtaaccggc gcgaggcgca ttgtcgtgtc cttgaaaact tccgtgaaaa cgccacccag
      961 accgaaaaca acacccggac cgaacccggc aaagcgcgtc ataccggcca aaaattcgcg
     1021 gctgccgctg agcatttcct gcaccagcac cggcgcagtt tttcccgcgt tcttttgaat
     1081 attttcaaat tcttttttga gctggttttc gttttccaca ttcagcgcga tgagtccctt
     1141 gccgcttttg tgcatgattt tccaggagca ggctttgagc gccaccggat agccgatttt
     1201 tttagcggtc ttcagagcgt cttcaacctt tgccgccagc gcttccttcg ttaccggcac
     1261 accgtagcag gcaagcaact gtttcgcctc gtattcatcc agcgcctttt gtttgttgtc
     1321 cagcgccttg aggatgatct ccgtggcctc ttttttgatt ttcggcaaag cggcttcttt
     1381 gcggggagcg cgctctttga tttgtttgta caggtaaagc gagcccagcg ccctcgcggt
     1441 attttccggc gaataaaaca ccggcgtgtt tgtatcctga tagcccttga tatagttgtc
     1501 ttcatgatca aagaaagaag atatcagcat cggcatgtta tacttagatg gcaatttaaa
     1561 agtttcgttg gcaatcgtcc ggtccatctg gccgtatttc aaaaaatctt ccagagaaat
     1621 attgccgata atgtccttca acgtgggata aacctctttc agataaccgg tcatcatggt
     1681 tccgtgcaga acgaccgcat cgacctcgcc gcttttcatc accatctccg gcagggtgag
     1741 cgcgagtttg ttcatgctca ggtcaaacgt catatcgacg ggattggacg cagaggcgtg
     1801 cggttcaatg tgttttctga tttcgttttg cagcccgtcg gaaaagcggg gcacttccag
     1861 accgaccgca tcgcaggtat aggaaatggt tgtggagggc ccgccggaat tggtcatgac
     1921 gccgactctt ttgccgcgca tcggcggttg tgtggccagc gtccagccgt gatagtaaag
     1981 atcttcaatg gtgttgaccc ggatgatgcc ggcctgcttg aaaataccgt tatagaggaa
     2041 atccggaccg gccatggcgc cggtgtgact caaccccgcg cgcgcgccgg aggcggaacc
     2101 gcccacgtat aacgctgcaa tcggtttgtg cggtgtgatg tttcgggcaa cgtcgagaaa
     2161 ccttcgccct tcgcggatgc cttcaatgta cagaatgatg gctttggttt gttcatcttc
     2221 tcccagatat tccagcgcgt caatcatatt gatgttggcc tcgttgccca gactgatggc
     2281 cttgctgaaa cgaatccctc ttttgttgag atagggcagc gtctggctca ggaatgtgcc
     2341 gctttgcgac gcaaatccca gaaggcccgg ttccctggac gtggctaaaa cggtggtgtt
     2401 taatgaaatc ccggaattga tgacacccat gcagttggga ccgacaaaac gcatctgata
     2461 gcggtctgct atttcgttga tctttttttc catgtcccgt cccgccgcgc cggtttcctt
     2521 aaacccggcg gtgattacga tggctctctt cgtgccgatt ttgccgaagc cctccagcag
     2581 ggaagcaacc gctcgtatgg gcacaatcag gatggccaga tcgggcgctt cgggcaaaac
     2641 ctccggtgtg gcgtaggcgg ggcaacccaa aacggttttt tccacgggat gaatcgggta
     2701 aaatctgccg ccatagccgc ccttcacaat attcaacgcc tgtatagttc ccatcttcga
     2761 cgggttgtta ttcgctccgg caaccgcaat ggattgggga ttcatcagca aatgcaatgg
     2821 attttccgac attattcaat ctcctttatc gttgtattga tgagcgcatc agcattgtgc
     2881 tttcgaagtg catcttcaat aaaaatggga cctgcttcag caagcatctt aataatttgt
     2941 ttatcctgat tgttttttat gtatccggca aaaaccatga cggcacatcc atgggtgtaa
     3001 atccagccgt ttttcaacag atcctttctc tcggccggag caagtttctt gagttccggc
     3061 accttcacga tctctttatc cagcttcttc agcatttcgt cgatgattcc gctggcatcg
     3121 ttatcctcca ggaatatcat tttaaaaaat tctttatgat cccgggcata caaaacatag
     3181 ccgacgccgg tattcaggaa ggcatttttc gtgtactgcc gcgtgacata atcatagaga
     3241 agatcaacgg tcttttgcag caccgccttt ttcaactcgt ccatggattg aaaatgttcg
     3301 tataccggcc gggtggagga aatcaattgc ctggcaactt cgcgggcgct cagttgctta
     3361 aatccctttt ctttgacgat tctcaacgag gaatcgataa tatcggacct gctataaata
     3421 acttttggcg gcatgcaatt cccggtctgt ttaaattaca tgtgtaatgt aacgtgtgtt
     3481 atgtattatg tcaagcgttt attgattatg aaataataaa gaattttgaa tgcaggtctc
     3541 atttatcaac gaacgtcgtt attttcagac cttgcacggc gctcccattg cgattgacgt
     3601 cgcatggagg cctattcccc ttattatcgt tatacttgac tatttggcta aaaaaatata
     3661 gttaagcaga ctttacaaaa gtggagaagc tatggaagat aatgaacttt taaatatccg
     3721 ccgtgaaaaa attgccgatc tgaaatcggc gggcattgat ttatacccca acgacatcaa
     3781 accaaaaaac acaacacagg aaataacgtt gaaattcggc gatatggata acgattccct
     3841 ggcaaaacag gaagagactt tttccgttgc cggacgtctg atggccgtgc gcaatttcgg
     3901 caaagcggct tttatcaaaa ttcaggatta caagggacag atccagggct acatcggtaa
     3961 gaacagttta agcccggaag aatattttgt tttcaagaaa ctcgacatcg gcgacatcat
     4021 ctttatctcg ggtaaattat tccggacaaa aaccaacgag ttgaccattg aagcgcagaa
     4081 gctcatgctg ttatcgaaat cgatacgtcc cttgcccgaa aaatggcatg gcctgaccga
     4141 catcgaaaca cgataccgcc agcgatatct tgatctgatc gccaacccca aggtgaagga
     4201 aatatttcac cggcgcagcc tcattatcaa gttgatccgt cagttcatgg atgagcatga
     4261 ttttctggag gtggaaaccc cgatgatgca gccgcgcgcg ggaggcgccg cggcccggcc
     4321 gtttaaaacg catcataacg ctctggacat ggatttttat ctgcgcattg caccggaact
     4381 ttatttgaaa agactggtga ccggcggcct ggagcgggtc tacgaaatca accgcaattt
     4441 ccgcaatgag ggcatctcca ccttccacaa tccggaattc accatgatgg agttttatca
     4501 gtcctatgcc acgtatgaag atttgatggc ctttaccgaa gacctgttct gttttctggc
     4561 gaagaacata ttcggcggtc tgaaattcaa ttatcaggat acggaaatcg atctgacccc
     4621 tccgtggcgg cgcatttccg tcaaagacgc tctcctgcag ctggcgaaca tggacgcggc
     4681 ggcgctggac gatccggcgc tggctttatc ttttgcccgc aaggttggtt gcaaggtgaa
     4741 cgacaatgat ccgttgggaa aaatcttaat ggctattttt gacgaggtgg tggaaaagaa
     4801 actgatccag ccgacatttg tcacccacta cccggtcgtc atttctccgc tctcccggcg
     4861 atccaaaaat gatcccgatc tcaccgaccg gtttgaactg tacatttacg gtcgagagat
     4921 tgccaatgcg ttttcggaat tgaatgatcc ggtcgatcag aaaggacgat ttctgcagca
     4981 gctccatgag cgggaagccg gcgacgacga agcccacgaa atggacgagg actatgtccg
     5041 cgctctggaa tacgccatgc cgccgaccgc cggagaaggc atcggcatcg accgtctggt
     5101 gatgcttttt accgacagtt cctccatcag agacgttatt ttattcccgc tgttgagaac
     5161 cagacaggaa taaacggatt attgacggcg ttgccgaacc ggacttatcc cggaaaaagg
     5221 atataacaat accgatgccc tacgaacttt tcatcagcag gcgttattta aaggccaaac
     5281 gcaaacaagt ttttgtttcc gtgataacgt ttatctccgt cctggggatt tttctcggcg
     5341 tcgccgccct gatcattgtt ctggcggtaa tgaacgggtt tgaagaggat ttgcgcacaa
     5401 agatcctggg cattaagtct cacgttgagt taaccaccga tatgagcggc cccatgaaaa
     5461 attaccggga cgtccgtaaa aaaattgccg gcatcgaagg cgtggtcgcc tccacgccgt
     5521 ttatttatac ccaggcgatg atccgcaacg acaacggcgt caccggcgtc gtcattcgcg
     5581 cgctggatac cgaaagcgcc tttcgtgtca tcaatctggg aaaaatcaaa gaagggaatc
     5641 ttgatcatct caacaaaatt ccgcaggaca ttgccgccgg ttattcagac aacggcgtcc
     5701 agccggacgg tattgtcatc gggaaggaaa tggcccggaa catggggatt tttctctacg
     5761 acaccataac gatcatttcc ccggtcagca ttgccacgcc ggcagggatg gtgccccgca
     5821 tggggaaatt cgtggtggtg ggaatttttg aatccggatt ttacgaatac gattcaaccc
     5881 tggcgtatct gtctttgccg agctgccaga aatttctgca aatgggagat acggtaacgg
     5941 gaattgatat taaagtgaac gacatttaca aggcggatgt cattgccagg aaaattcaaa
     6001 gcgctctggg attcccctac tggacgcaaa actggatgca gatgaacaag aatctttttt
     6061 cggcgctgaa gctggaaaaa cgggtgatgt tcatcattct gagcctgatt gtgctggtgg
     6121 ccgcctttaa tattatcagc gctttgatca tgatcgtcat ggaaaaaaac aaggatatcg
     6181 ccatattgaa atcgatgggc gcgacctccg caagcatcat gaaaatattc gtttatcagg
     6241 gattgatcat cggtgtgatc ggtacttttc tgggatgtgc agcgggatta gccgtcgcat
     6301 taaacctgca gaaggtttcg ttgtttgtgg aaaagatttt taactttaaa tttttgccgg
     6361 gagacgttta ttatttgagc gaacttccgt ccaaagtgaa ctacagcgat gttgccatta
     6421 tcgtggccgg cacgttgctg atttgctttt tatcgaccat ttatccgtcg ttgcgggcgt
     6481 ccaaaaccga cccggcggaa gctttaagat atgaataata acccgacaga cgacgcaaaa
     6541 cgatgatagt actggaaaat ttatcaaaaa ccttttttaa agccggaaac aaaattgagg
     6601 ttctgcgcgg tctgaatttc aaaattgaaa agggccaatc ccttgccgtt ctgggcgcct
     6661 cgggcgccgg gaaaagcacc ctgatccata tcatgggcat gctcgatcac cccacgggcg
     6721 ggctgctgct tcttgacggc aggaatgttt ttgcctggga ggaaaaaaaa agggcggcct
     6781 tccgtaattc gcacatcgga tttgtgtttc agttcaataa tcttctaccg gaattcaacg
     6841 cgctggaaaa cacgatgatg ccggccctga tcggaggatt caacaagaaa caggccggag
     6901 aaaaagcttg caaattgctt caagaagttg gattagagag cagaatcact cacaagccgg
     6961 gagaactttc ggggggcgaa caacaaaggg tggccattgc ccgcgccctg atcatggagc
     7021 cggacatcct gctcgttgac gaacctaccg gaaatcttga cacggaaaca ggaaaaaaga
     7081 tagaagacat tctggtcaac ctgaatgtga caaaaaaaat aacgcttgtt acggtaacac
     7141 acaacaaatt actggcggaa agaatgtccg aaaggattct tctgcgcgat ggagaaattt
     7201 atgaatacca gtaaaaaaag ctttaaaata tctttggctc ttttattgtt tgtctttctt
     7261 tttgttccgc cggcttattc ggaagatctc aaaaagattg ttgtgatgcc ctttgaagta
     7321 ttcagcaatc aggataaaac ggtcatccgg gaatcttttt acaaaaacct gcaggcagaa
     7381 ctgcagaaag aaaaatccat tcagattatt gccgccgacg actttctgca acgcgagggg
     7441 cagattgacg aaaatacggc cctggatcac ggcaaggcaa aaggcgccga ttttatcgtc
     7501 accggcaccc tgactcaatt cggcaaatcc atgaatatcg actgcaaaat attcgatgtg
     7561 gaaaagcaaa gttcactgcc cgccgtgtcc acgcatggcc gagggatggc cagcacaacc
     7621 gctcttgcgg aacaattaaa agtcgaaatc ctggaccgca tcggcattgt gcagaagatt
     7681 gcccgcattg aaattcaagg caaccggaaa atcaatgcct ccgcgatcac gacaaaactt
     7741 aaaagcagga aaggcggtat tttttccgaa gccgacattg cggcggatat taaaaccatt
     7801 ttcaaaatgg gatattttct ggacgtcacc gctgaaacgg acgccacgcc ggaaggcaaa
     7861 gtcatcacgt tttttgtgca ggaaaaaggg ctggtgtccg aaatcgtcat caacggcaac
     7921 aaggccgtca gcaaagacga cattcaggaa gtcttaacca tcaaaacccg gcagagcctg
     7981 aatcgggaac aaatcaaggc agacattgaa aagatcaaag cgctctacga cagcaagggg
     8041 tattataatg cggagatcaa agaccgggtg gaacaggacg gggaaacaga tttccgtgtc
     8101 gttctggaga tcagggaaaa cgacaggctc tacatccgat cgatcgcgtt tgaagggaat
     8161 gaagcctttc cggccaagga attgaaaaag ttgatgagca cctcggagaa aggattcttt
     8221 tcgttcatca ccgattccgg gttgctcaag cgtgaccagc tcaagcagga tatcggaaaa
     8281 atcaatgcct attattttaa taacggattt attaatgctc aaatcggcga accggaaacc
     8341 acagccgatc agaaaggcat ccacgttaaa attaaaattc aggaaggcaa gaggtttaaa
     8401 gtcgataatg tggccatctc cggcgatgtt ctggaaaagc cgcgcgaggc gctgctgcag
     8461 tccctcaaag tgaagaaagg cgataattac aaccgggaag ccatcatcag ggacctggat
     8521 cttctgatgc aagcctgcaa cgatgaaggc tacgccaacg cggaaatcaa tcccaagatc
     8581 gatacgcggg aaaacgagca attggttgac gttgatttcc agattgccaa gggggaaccg
     8641 gtttatatca gcaacatcaa catttccggg aacagcatca cccgggacaa ggtcatccgt
     8701 cgtcagctgg acgtggtgga aggggatctc tattccagca gtaaactgag aagcagctat
     8761 gccaatctga gcaggctgag atattttgaa gaggtggatt tccagacgga aaagggaacg
     8821 gataaaaaca aaatggatat caatgtccgg gtcaaggaaa aaagcacggg catgttcatg
     8881 gtgggagcgg gttacagcgc atcggataaa ctcgtcatga tggcacagat cacgcaacag
     8941 aattttctcg gctatgggca ggtattgagt ttgaaagctt cgctgggctc cgaaaccagc
     9001 aattacgatt tatcctttac ggaaccatgg cttttcgacc tgccgctgtg gtgcaaggcc
     9061 gatatctgga aatacgagaa ggaatacgat tattacacca tcgacacgca gggcgcgggt
     9121 ctgacgctgg gttatcccct gattgaacgg attgtcggct atctgggcta caagtattat
     9181 atcgacgata tccgggaggt ttcgccgatc gcaacagact acgtcaaagc acaggaaggc
     9241 gaaacatctt ccagtgccgt caccttgtct ttgattcgcg atacgacaaa cgattacatt
     9301 ttcccctcca agggaacgaa ggccacggtt tcggtcaccc acgcgggcgg ccccctagag
     9361 ggagatgcca actacaccca gtacggagca agtctcttcg cttatttccc tctgcccctg
     9421 gacgttgttt tcgcagccaa gggacggatc ggttatattc aggatcatga aggggttgaa
     9481 attcccgttt ccaacagata cgttttgggc ggaatcaatt ctctgcgcgg cttccgctat
     9541 gtcggaccga ccaacggcgg gacgtccgat gtcatcggcg gtaccaatat gatggttttt
     9601 aatgttgaac tggtattgcc ttttatcaag gaatccggca tgaagctggt cgccttttac
     9661 gatgccggca actcgtggga caataagtac gatatgggag atttaaggca atccgtgggc
     9721 gccggaatcc gctggtactc accgatcggc ccgctgcggc tcgaatacgg tcacgttctt
     9781 gaccggggag agttaaacga cgattccaaa ggacgctggg aatttaccat cggcatgccg
     9841 atgtaaaagc atagataaaa aattaaacat ataatttaaa tgaggagacc acaaatgaaa
     9901 aaattttttt acctgacggc gattatgaca atgctggttt ttgcatggag tgcaaacgtt
     9961 ttagctgctg ataaaatcgg attcatcaat ctgcaggaaa tcatgcagac gtccaatgcg
    10021 ggcaaaaaag ccgccgacga attcaagcga ttttacgaga aagaaacaca ggaaatcaaa
    10081 agcatggaaa accagctgaa aaaaatgaaa gatgagctgg aaaaacaggg ttccgttctg
    10141 acccagagct cacgcagcga aaaggaagcc gcttaccaga agaagatgcg ggattatcag
    10201 ttgctggtca atgacactaa tgaggaatta aaaaaacgcg atcaggaaat gacccagaag
    10261 ctgatgcccg gtattattaa aatcgtccgc tccattgccg aacgggaaaa gtacacgctg
    10321 gtgattgacg tggcgaccat gccgattcct tactatgcca aggaaaacga tttcagcaat
    10381 aaagtgatcg aagaattcaa caagaccaaa taacaccggc acgttcaacc gatgcaaatg
    10441 acgactggag aaatagccgc gtttcttggc ggcactgtta tgggcgcgga tgacgttgtc
    10501 atcggccata tccgggctat cgatgaggcc ggtccgggcg acctgacgtt tattgccaat
    10561 aagaaataca taaaaaaact gaaaacgaca aaagcgtccg cgattctcgt ttctccgcag
    10621 acaacggcgg aggggaaaaa cctgattgtc gtttccgatc cttatgtggc cctgggaaaa
    10681 atactgagcc atttttatcc ggcgcaacac ggccggtccg gggtcagcgc agaggcctgc
    10741 attgaagacg gcgccgatgt ttctccggac gccgttattt ttccccgtgc ttttatttca
    10801 gccggagcca aaatcggtgc gggaacggtt atttaccccg gcgtctttat cggcagaaac
    10861 gcatccatcg gcgaacattc catcatccat gccaacgtca ccgtttacca ttcctgcatc
    10921 atcggcaacc gggtcattct ccattccggg gcggtgattg gcgccgacgg cttcggcttt
    10981 gcagcgccgg gacgcgacaa cgttaaaatc ccgcaggttg gctttgtgca aatcgacgac
    11041 gatgtggagg tgggagccaa cacgaccatt gacagagcga ctctgggcag gacctggatt
    11101 caacgcaacg tcaaaatcga caatctggtc caaattgccc ataatgtggt catcggtgaa
    11161 aattcggtca tcacagccca ggtcggcatt tccggaagca cgcagctggg gaaaggcgtg
    11221 atggtcggcg gacagaccgg tatggtcggc catataacca tcggcgacca tgtcatgatt
    11281 gcggccgggg cggggattca caaagacatc aaatccggcg acatggtggg cggcgcaccg
    11341 cagttgccgt ataaggaatg gctgcaggtg gaagcctgcc gcgccaaact gcctgaaatg
    11401 agagcgacgc tgaacgatct tgtgaagcag gtggaagagc tcaagaaaaa gtaaattctc
    11461 ctaaagaagg ttaactgatg aaaattcacg aaacagctat tattgcaccc ggcgctcaac
    11521 tggaagaagg cgtggaaatc ggcccctact cgatcatcgg cgccgatgtc aggataggga
    11581 aaaatacggt gatcggtccg catacggtca tcgacgatta tacccaaatc ggcgaaagct
    11641 gccggatctt tcagttttgc tccatcggag cgcctccgca ggatctgaaa ttcagcggag
    11701 agaaaacccg ggtggtgatc ggcaatttca acacgatccg cgaatttgtg accattcatc
    11761 gctcgacaac agccgacatc ggcgtcacca tcatggggaa tcataatctg attatggcct
    11821 attgccacgt ggcgcacaat tgcaagctcg gcaatcacgt gattatgtcc aatgcggcaa
    11881 cgttggccgg ccatgttcat gtcgaagatt acgccattat cagcggactg accggcgtgc
    11941 accagttctg ccgcgtcggg gcgcactgca tgatcggcgg atgctcggcc gccgtaaagg
    12001 atattccccc gtatacgatt gctcagggca atcatgccaa actttacgga ttgaacctta
    12061 tcggattgaa aagaagaaat tttccggaaa aaaccatcaa ggccatcagt gatgcctacc
    12121 gcattgtttt tcgttccaag ctgctcctgg aagacgcgat caaaaaagcg gaatctgaag
    12181 ttgaggatct gcccgaagta aggcatttca taaaatttat taaagaatcg gaaagaggca
    12241 tttgccgttg atttacacaa catgaagcat aaaaaaatta aagtcggtgt catcggaatc
    12301 ggccacctgg gcaattatca tttgcaaaaa taccggaaac tggaaccctg cgaaatcgtc
    12361 gccgttgccg acacgtcttc ggagcgcgct caaaaagcag cccagattta tcagtgcgcg
    12421 gccttcagcg actaccggga aatgatcggc agcgtggatg ccgtcagcat tgccgtgccg
    12481 acaagcgacc attacaacgt cgcgagggag tttcttgcgg cgggcgtgga tgtcctcatc
    12541 gaaaaaccgc tttgtgcaac gcttgcggaa gcggatgatc tgatcggtct ggcgcggaaa
    12601 aagaaactca ttctgcaggt cgggtttgtt gaacgattca atcccgccat catggcgctg
    12661 gaaaacgtca tccggaaacc tctttttatc gaatcccata gattacaccc gttcttcgaa
    12721 cgcggcacgg atgtggacat catcctggat ctgatgattc acgatctgga tatcctcgta
    12781 aaattcgtgg atgctccggc gatcagcgtg gaggccatgg gggttcccat tctctcggat
    12841 aaaattgaca ttgccaacgc ccggattact ttttccaccg gctgcaccgc caatattacc
    12901 gctagtcgga tcagcgccaa gaccatgcaa aaaatacggt ttttcagtcc agaaggctat
    12961 cattcggttg attgccaaaa acgggaagta ctgtccttca gtaaagggac aaccggctcg
    13021 ggccagatgc agattgtcca aaaccatctt gaaataggca gccacgatcc gctggaagag
    13081 gaaatccgct cttttgttga cgccgtcatc acccgcggct gcccgccggt ttccggggaa
    13141 gaggcgcgca aatccctggc gctcgccgtg gacatcatca caaaaatgaa agcagcagag
    13201 gatttactga catgataccc atggtcgact taaaaagaca gtaccacagc ctcaaaaggg
    13261 acattgatgc cgccatcagc aatgttctgg aagatacccg ctttattctc gggcccaacg
    13321 tgtccgcgct ggaagaagaa gtcgccgcct atcacggtcc gtcccaggcg gtgggcgtgg
    13381 ccaacggaac ggatgccctg cttctggctt tgcgggccag cggcattgaa gccggtgacg
    13441 aggtcattac cacgcccttt acctttatcg caacggcaga ggtcgtcgct cttttgaaag
    13501 cccggccggt ttttgtggat attgagcccg tcacgttcaa catggattgg cgtcaaatcg
    13561 aaggcaagat aacctcgcgg acgaaagcca ttatcccggt tcatctgttc ggccatcccg
    13621 cggatatgga tcccattatg gccatcgcta aaaaacataa tctccgggtc atcgaagact
    13681 gtgctcaggc atttggagcg gtctatcacg gccggaaagt gggggcgatc ggcgatatcg
    13741 gttgtttcag ctttttcccc agcaagaatc tcgccggtta cggcgacggc ggcatgatcg
    13801 tcacccgaag cgacgagacg gctgcaaaga taaaaatgct gcggaatcac ggcagcgcca
    13861 gacgttacta ccatgatgag gtcggctata acagccgtct ggacgagatc caggccgcca
    13921 tcatccggat caaactgaaa aaaatcgacg aattcaacgc ggcccgccgg cgcaatgcgg
    13981 cgctttattg cgcagccatc aaacgcaagg acatcgtttt gccgtccgct tccggcgatt
    14041 gcgaacatgt ctatcatcag tttaccattc gctcggacga gcgggatctt ctggccgacg
    14101 cgctgcagaa aaaagaggtc gcttccgccg tgtattatcc tgtgccgctt caccggcaga
    14161 acgcatttct caacctgtac gacggaccgg aaacgatgcc gcaatccgaa acatgcgccc
    14221 gggaagttct ttccctgccg atgttccctg aattggatca gaaagaaatc gaattcattg
    14281 ccgatatcat taacaaggca tcctgaagaa aaatccggct gagatcatga aagattcaaa
    14341 tcataaaaat aaaaccgtgt tgatcatcgc cggcgaagcc tccggcgaca tgcacggcgc
    14401 caatcttgtg cgggaaatgc tgaatcgcga tccggcgctg aaattttacg gcatcggcgg
    14461 gaaaaagatg ctgcaggaag gggttcagct tcttgccgac gcctccacga tggcggttgt
    14521 cggcctgacc gaggtcgttt tcaaattaaa agacattctg aaaatcatga ggacgataaa
    14581 aaaatccctc gatgaacggc gtccggatct tgtcatcctg attgattatc cggattttaa
    14641 tctgcccgtg gccaaagcag cccgtcggca caacatcaag gttttatatt acatcagccc
    14701 gcaggtctgg gcctggcgtc agagcagaat cgaacagatc aaaaagaccg tcacccggat
    14761 ggcggtcatt ctgccttttg aagtggaaac ataccggcgg cacggttttg cggcagatta
    14821 tgtggggcat cccctactgg atatgattaa gacaacctat tcaaaaaaag aatcgagaaa
    14881 aaagttcgat ctgcacgaag ataagatcac gataggcatt ctgccgggca gccgcatgtc
    14941 ggaaataaaa aaattgctgc cggaactgct tcaggccgcc acaatcatga aagacagcat
    15001 cccggagctg caatttgtcg tcccattggc cgacacgctt gaagagaaaa atctaaccgg
    15061 aatcattgcc ggatttccga tcgatgtcaa cgtcatttcc ggccagacct acgatgtcgt
    15121 ctcctgtgca gacctggcgc tggtcgcctc cggaacggcc acgctggaaa cagcgttact
    15181 gaaagtgccc atgattatcg tttacaaaat ttcgccgctg tcttatttta tcggaaaatt
    15241 attcgtccgc gtcaaaaata tcggcctcgt caacatcatt gccgagaaaa ccattgtgcc
    15301 ggaactgatt cagaacgatg ccagcggcgc ccggatcgcc agggaggccc tttccatttt
    15361 aggcgatgaa acaaaaaagc aggccatgat ccgggaactg gaggctatcc ggtcccggct
    15421 cggcgagccc ggggcggcaa ttaaaactgc taaactggca ttagatatgt tataggaaca
    15481 ccggaaacat catgaaaata tttaaacgtc ttttaagtct tggtcgtcca tacagcgtgc
    15541 gttttctgat ttccatgctc tgtatggtca tagccggtgc actgacatcg tcacaggcgg
    15601 tgctggtgaa acctgtgctg gacgacatct ttctggcaaa aaaccccgcg tcgctgaaat
    15661 ggctccctct cgccgtggtc atgatttttc tggtgaaagg aatctgtaat tacgttcaaa
    15721 cggttttaat gaattttatc ggcttgcgca ttgtcaccga tctgcgcaat cagctctacg
    15781 aacagataca gaaacagtcg ctgtcctttt ttacgcatca tccgacgggg ctgctcatgt
    15841 cgcgcattac caatgatgtc gcgtccattc aaaacgcggt gtccgatgtg gtcaccagta
    15901 tcgtgaagga ttcctttaca attgtcgccc tgatctgtgt cattttttat acggactgga
    15961 agctggcgtt cattgccatg attatttttc ctctcacgat ttatcctatt tccgcattcg
    16021 ggaagaaaat acgcaaggtc accacgtcga cgcagatcac catggccaat ctcagctcgc
    16081 ttttgcagga aaccattgcc ggaacgcgca tcgtcaaggc cttcggcatg gaaacgcacg
    16141 aaatcaaaag attcgccggc gagaacgagc gcttttttaa attgaacatg aaggccgttt
    16201 ccgtgaaagc cctctcccat cccctgatgg agtttctggg cggactgggt attgcggccg
    16261 tcatcttcta cggcggctac aacgttctgg ccggcaactc cacacccggc acgttttttt
    16321 cctttttgac cgctgtgctg atgctgtacg aaccggtgaa aagacttacc aacgtcaaca
    16381 acacgatcaa tcagggcatt gccggtgcgg aaagagtatt cagcatcatt gacctggttc
    16441 cggatatcca ggacaaaccc cacgccgccg aattgccgcc gattgaccgc ggcatcgaga
    16501 tcgataacgt gacattccgt tatgaaaaaa cgccggtttt gaaaaacata acgttgtcta
    16561 tacgggcagg cgaagttgtg gcgttcgtcg gcatgagcgg gggaggaaaa acgtcgctgg
    16621 tcaatttgat cccgcgattt tatgatgtca gcgaaggccg cgtgcttatc gacggtctgg
    16681 acattcgcga cgtttccctg ccgtcgctgc gccggcagat tgccatcgtc acgcagcaga
    16741 ccattctttt caatgatacg gtgagaaaca atatcgccta cggcagtttc gaaaaaagcg
    16801 acgacgagat tatctgcgcc gcgaaagccg ccaatgccca tgatttcatc atgagaatgc
    16861 ccaaagggta cgacaccaac attggagaac tcggcacgaa aatttccggc ggagagaaac
    16921 aacgtctttc catcgcccgt gccattttga aaaacgctcc cattctgatt ctggacgagg
    16981 ccacctcctc gctggacacg gaagcggaaa tggaagttca ggaagccctg gataacctca
    17041 tgaaaggcaa aaccacgctg attatcgcgc acaggctttc caccatccgc aacgccgaca
    17101 gaattattgt gctggtgaac ggtgaaattg ttgaagaggg caatcacgaa acgctgatgc
    17161 agaaacaggg tgaatatttc cgactgtaca atttacagtt caaggatgaa ggaagtcatg
    17221 aaaacaataa ccgactggcc aaggatttgg gataacgacg gaagcgcaag cggttacggc
    17281 acgataaaat ttattgcgca tcttttatct ttcttctatc tttccatcgt caatttacgc
    17341 aactggctct acgatcacaa cctgttaaaa gcgacccggc tgggctgtcc ggtcatcagt
    17401 gtgggcaata tcacggtcgg cggcacgggg aaaacaccct gcgtggtcat gctggcgcgc
    17461 atgcttcagc accggggctt taaaccggcc gtgatcagcc gaggctacgg cggacgcgga
    17521 aaaggcgccg tgaacatcgt atcggatgga cagcgaatcc ttctggacag cgaaatagcc
    17581 ggagatgaac cgttgctgat ggctcatgct ctgacgggca ttccggtcat taccggaccc
    17641 cgaagaacag caaccggcaa aaccgccatc gaccacttcg gcgcggatgt gctgatctgc
    17701 gacgatgcca tgcagcaccg gcagattttt cgcgatgtca atctggtatt aatagacggt
    17761 caggatctga aagacaacat ccatgttctg ccgcggggca gattgcggga accggtaaaa
    17821 gcactccggc gcgccagtgc cgttgttttt acacgcgttg acgggcagat cgcggtccat
    17881 caaacgatag aaaacctgat cacaaaggaa aaccttcctg tttttcaaag tcttcaccgg
    17941 ataaacgata tgatcagcgc ggataaaaaa atttgtaaac cgtttactga gctggaagga
    18001 aaaagggtcc acgcgttttg cggaatagcc aatccggagt cgtttaaaaa gacgctgctg
    18061 tcaaccaaag ccgttttggt tgcgtttaat atttttccgg atcatcaccg ttttcaggaa
    18121 cacgaactgg aaaaaatcaa aaacgatttt aaaaacagcg cggcggacta tctgatcacg
    18181 acagaaaagg atgcgatgcg tttaaaaaat catccggaaa tgtcaaaaat gcttttcgtc
    18241 ctgcgcataa caatggagat caaagacaat ccgcagtcgt ttgaaaattt tatcttacac
    18301 aagatccggg ccgggacgaa aaagggatga agggggttct ttgaaagacg aaaaaatcaa
    18361 aaaactgccc caaaaaggca tccataaaat tctgattcgc ggcaccaact ggatcggaga
    18421 tgcaatactg accttgcctg ccgtagcctc catacgcgcc acttatccgc aggcccggat
    18481 tgccatgctc gtcaagccgt gggtggcgga tgtttatgac attttttcca acctcgatga
    18541 agtcatcgtc tatgaaaaca aatatgacac ggccaccggc gtattcgggc tcgcccggat
    18601 gctccgggaa aagcaatttg acgcagccat cctcctgcag aatgccatag aggctgccat
    18661 catcgcgctt tttgcaagga ttcccgtccg ggcaggttac aattcggatg cccgcggcct
    18721 tttgctgacg cacagcgtgc cccttacccg cgacataaaa aaggtccacc aggtgaacta
    18781 ttatctggaa atggttaagg cattgggctg cgtttccgcg aaccgggaca tgaatctgga
    18841 aacaaaaatc agccgcgagg aggcacaaaa cgttttgcgc cgctatgtcg caccgtcgca
    18901 gaaaatgctg atcggaatag caccgggcgc cacctatggc gctgccaaaa aatggttccc
    18961 ggaccgtttt gcggaaacgc tcaaccggct ggatgaacaa ttttctgtgc agggaattat
    19021 tctgggaggg aaagctgacc gaataacggc cgaggaggtc cggacatcgg cccgcgccga
    19081 tatgatcaat ctggccggcg aaacctcttt gcgggaggcg atttatctga tttctcaatg
    19141 ccgtcttttt atcagcaatg attcgggatt aatgcatgtg gcaggcgcgc tcaatgttcc
    19201 aacgatcgcg atattcgggt caaccaatcc gacaaccacg tcgcctgtgg gaaagcaatc
    19261 cttgatcgtc cgcaaggaag tttcctgcag cccctgcctg aaaaaaacct gtccgacgga
    19321 tttccgctgc atgaacttga ttaccgttga ggatgtcctg tcggtcgctc aaacattgct
    19381 gaaggataac cgggataaag atcaatgaaa aagaatacgg cggtttttct ggacagagac
    19441 ggggttatca atgaagaggt cggttacctg gacggccttg aaaaactcaa agtgattccg
    19501 aacgcctgta aggcaattca gatgattaat caaagcggca tgaaagccat cgttatttca
    19561 aatcaatccg gcgtcgcccg aggattgttc acggaagaat tcgtgcaaac cgccaacagc
    19621 catttacaga aaattctcaa aacacacgga gcctctattg atgattttta ttactgtccc
    19681 catcatccct ccgaaggcaa agcgccttac cgccaaattt gcgattgccg caaaccggct
    19741 ccgggaatgc tgctgcgcgc ggcgcgggat ttaaatatca atcttaccct ttcttatctg
    19801 gtgggcgacc gatacatcga tatgatcgcc gccagacgag ccggggtgaa aggtattctg
    19861 gtgaaaacag gctacggaaa cgacttgctc caggacgacg gaccggacag gccaaccccg
    19921 gagagcgaac ctgattttat tgccgccgac attctcgagg cggtacagtg gatattgaag
    19981 gacaaaacgt gaacatcctg attgtaaaat taagcgcaat aggcgatgtg attcacacgc
    20041 ttccgtctct ggccgagctg cgccggctct atcccgacgc ccatatcacc tgggtggtgg
    20101 aagaagcggc cgccgatctg gtcaaacatc acccctgtct cgacgaggtg ttgatttcac
    20161 gccgcaagac ttggatacaa aatgtccggg ccgggaaaat cagacagtcc ctcaacgaag
    20221 gcatctcctt tatcaaaggc ctgcgaaagc gccattatga tctggtcatt gattttcacg
    20281 ggctttttaa aagcgcaatc attgtctttc tcagcagagg cagaaggaaa ctgggatacg
    20341 attcctggca ggaactcagc ggtttgtttc ttaatgaaaa gattcccgaa gacatggata
    20401 aacacgctgt tgaccgctat ctggattttt tgcggcacct tggcgcaaaa acgccggagg
    20461 ccaggttcgt tcttccgtca aacgacgagg aagaaaaaaa agcacaactg ctcatgcaga
    20521 aacaccgcct ggaagacaaa aaattcatcg cggtcaaccc ggttgcccta tgggatacaa
    20581 aactctggag caatgaaaaa ttcgcccggt tggccgacct gattcaaaac cggctgaaat
    20641 tgaacgtggt gtttaccggc agcgacaaaa agccgctgga agcaataacc gctttaatga
    20701 ccaccgcagg cctgcacctg ggtggggaaa caaccctgat ttgtcttgcc ggtatttata
    20761 agaaagcgcg agcggtcgtc accacagatt ccggccctat gcacctggcc gccgcagtcg
    20821 gaacaccggt aattgctgtt ttcggaccga cggaccctgc ccggacgggg ccctatggat
    20881 caagacacac ggtcatccga tccggagaag attgcagccc gtgttttttg aagacatgtc
    20941 cggaaaaaaa atgtatggac catataacac ctgaacaggt ttttgcagcc gtagaaaaaa
    21001 cggtcatgga ggaaaaaaat ggcgttaaat aaaaaactgc tggaaattct tatctgcccc
    21061 aaatgccgcg gcaaggttcg ctttgacgaa aaagccaatg gcattatctg cgataagtgc
    21121 aaattgctgt atgaaatcaa ggacgatatt ccggtcatgc ttgtggaaga agcaaaaaac
    21181 attgactgag gcggatgttt tttccttgac ggttgtgata ttgaaggcct aaaataaatt
    21241 ttcttgacag cgaaattata atcatataag tttattggca acgagaaaag gctatttagc
    21301 cctacccttt ggagcgtttc aagtattcaa atacacaagc cgaaactaaa tccgtaatta
    21361 ctaaaaatct tagctatctt tattttgttt ctttaaacac aatgcagtaa agccgagaca
    21421 aatatccaat atcagggttt ctttatttaa cccgattcca aattaaggca aggaactatt
    21481 atgaacatcg aagacattaa gcgattgccc atcagcgagt tgaatgaact gggcaaaaaa
    21541 ctggacgtgc cgggagcgag cgggatgcgt cgtcaggagc tgatttttgc catcctgcag
    21601 acgcaggcgg aacaaaacgg cgttatttca ggctccggcg ttctggaaat tttgccggat
    21661 ggattcggct ttttacgagc catcgattac aactacctgc ccagtcctga cgatatctat
    21721 atttctccgt cacaaatcag gagatttaat ttaaaaaccg gagatacggt ttcgggcgaa
    21781 gtccgtcctc cgaaagaagg cgagaaatat ttcgcccttt taaaagtcga tacggtaaat
    21841 tttgaaagtc cggaacaggc ccgtgataaa atcctttttg ataaccttac ccctctgtat
    21901 ccgcaggaaa aattaaatct ggaatatgat ccgaagaatt actcaaccag aacactggat
    21961 ctgttcgcgc ccattggcaa aggacagcga ggattgattg tttctccacc gcgaaccggt
    22021 aaaaccgttc tgcttcagga tattgcccac agtattgccg ccaatcatcc ggaagtcgtt
    22081 ctaatggttc tcctgatcga tgaacgtccg gaagaagtta cggatatgca gcgcagcgtt
    22141 tccggcgaag tcatctcttc cacctttgat gagccggcga cctgtcacgt gcaggtagcg
    22201 gaaatggtta tcgaaaaagc caaacgtctg gtcgaacata aaaaagacgt ggtgatattg
    22261 ctcgacagta ttacccgtct ggcgcgcgcc tataacacgg ttgttccgcc cagcggcaag
    22321 gttctttccg gtggcgtgga ttccaatgcc ttgcataaac ccaaacgctt cttcggcgca
    22381 gcccggaata ttgaaaacgg cggcagcctg accattattt ccacggcatt gattgatacc
    22441 ggaagccgga tggatgaagt catttttgaa gaattcaagg gcacgggcaa catggaactt
    22501 catttggatc gccgtatttc cgaccgcagg atcttccccg ccttcgacct gatccgttcc
    22561 ggaaccagaa aagaagaatt gctgatcccc aaaaacaatc tcaatcgcgt atggattctg
    22621 cgccggctgt tgcaggaaat gaatcccgtc gacgccatgg aattcattgt cggcaaaatc
    22681 aagaaaacgg aaaacaatca ggaatttctg gattcaatga attcgtagcc gcataaaaat
    22741 gttttctatt tttacttgaa tttttgttcc atatattgta aggcagggca acgtttggcc
    22801 aagaagacaa aaaacactaa tgattaatta tagataaaaa atcagggagg aaaaataagt
    22861 atcatgaaag agggcattca tcctgactat caggaaacaa caattacctg tgtttgcggt
    22921 aatgttatcc atacaagatc caccaaaaag gacatcaaaa cggaaatatg ttccaattgc
    22981 catcccttta tgaccggcaa gcagaagatt gtcgacacag ccggccgtgt ggaaagattc
    23041 aagaaaaaat acgaaaccaa agttgacgca acgacgtcca agaagaaaaa atcttgataa
    23101 aataacatct tatcagagaa gaaccttcat gtcgcaacat ccacgaatga agaaaaagca
    23161 atttccctgc acgctgctcg atccggaatt ttgccttgat catgccaaga aacatgtttg
    23221 cctgattttg aacaactttc gttatctttc agaaacgttg ttaaacgcct ccctgcatcg
    23281 ccatttatag gtttccttca aaactcagga aggaagcctg tcaaacgtaa cccaaaacgc
    23341 cttaatcatt acatttatta ttgaagacat aaaacatgga tattatacgg gacaaattgc
    23401 acgatatcga actgcgctac cgggaactgg agggacaact cagcgatccc aaaatcgttt
    23461 ccaagccggg aatgtatcag aaatatgcca aagagtatgc cgatctgaaa gagctggtgg
    23521 aaacgatccg cgaatataat accctcagaa aacagataga cgagtaccag gatgttatga
    23581 cggaaggtga cgaagaactc aaagcgatcg tcaaggaaga attaccgcaa ctcaagcaac
    23641 aacaaaccga ccttgaagac agattaacca tcctgctttt acccaaagat cccaacgaca
    23701 ataaaaatgt ctttctggaa atcagggccg gaaccggagg tgacgaagcg ggtctttttg
    23761 ccgcagacct gttccggatg tacgcccgct atgccgaagc ccagggctgg cgcgtcgaag
    23821 tggtaagcag ctctcctgcg ggcggcatgg gcggatacaa ggaaattatc gcacagatcg
    23881 aaggcgacgg cgcttacagc cgactgaaat acgaaagtgg cgttcatcgc gtccagcgcg
    23941 ttccggtaac ggaagcccag ggaagaattc acacctccgc cgtcaccgtg gccatcatgc
    24001 ccgaggcgga agaggtcgag atcaatatcg atcccaacga tctgcgaatt gacgtttacc
    24061 gctccaccgg tcatggcggc cagagcgtca acacgacgga ttccgccgtc cgcatcaccc
    24121 atctgccaac cggactggtc gtgacctgtc aggatgaaaa atcccagctc aagaacaaga
    24181 acaaggctat gaaggtttta cgggcgagac ttttggatgc cgaggtgcag agacagaatg
    24241 ccgaacaggc gcagac
//
LOCUS       DAONQZ010000032        24152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2323_length_24152_cov_16.764037, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000032 DAONQZ010000000
VERSION     DAONQZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24152
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_24152_cov_16.764037"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..2034
                     /locus_tag="PLR13_06785"
     CDS             208..2034
                     /locus_tag="PLR13_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQC19080.1"
                     /translation="MAHSNWLYQTRDIMFQIKEWLGVEKLLSLDAYKDYYGMDDINSI
                     LDVNYKVCRDVMCPANKDADEPGAKFVGGNEHAVVTPDSFKNVYKTVMDAELGPQFGY
                     RGEGKIPLCWYAPILEMQSAASPSIVMFWCLTQGAITVLQEFATQKQQDLFIPKLRTG
                     EWGGTMGLTEPGAGSEVGACKSRAIPTDVPGKYKLTGTKCFISSGDHDLTENIIHLML
                     AKTPGAKEGTAGISLFIVPKFWVNDDGSLGAWNDVTTMGIEHKLGIHGSTTATLSMGE
                     NDNCYGWMVGEAEVKDGRGVGMKQMFAYMNEERLNTGLFSLGCMGSAYYAALEYAKIR
                     VQSKHSTNPKGPSVRIIEHEDVRRMLMFQKSMIEACRALIYKSYLYRDLAVDAATPEE
                     RDYYDDMFAINNPLCKAYTSDMARLTTGDAIQCHGGYGFTEEYAPAELYRDVVIYGIW
                     EGTNFIQAQDYTGRKFTMKDSVPFTKWVAEIDAFVVGKKTDEFAAEFAMMADAMVAFK
                     DIVEMNAAWTKGDKQLKQLYATRTMHAGARVYCGMLLLDQALLAARMLKEKGDVDADF
                     YKGKIATARFYVMNHVPDIFGYLKTMKCGDRSAIDIPEESFM"
     gene            complement(2478..3713)
                     /locus_tag="PLR13_06790"
     CDS             complement(2478..3713)
                     /locus_tag="PLR13_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19081.1"
                     /translation="MISLEENYGKNILPLSLVDNDRFPGGRPVELVLFYKEKNPLSKF
                     KECLFKAIEHYNLFSSRLIMTDKNKFALLYCTDGVQFNILPPIIAASDEVEIDDIKNR
                     MAHVKTLPGEPLFAVTGIPVNDGIFGGVSCSHTIADGISLMLFLYAWGCALQGKSYPL
                     PSTQRLFRGNPVCFDKMDKSFIPPLSELNEKIQKRVHAINAKRYFKREYFPDAFLNET
                     KKKAKEENKKYIISNNQIITSFLLKKYHHLMMPDADKIKIKIPVDFRGIHPDIDPLYI
                     GNVVFDSFTEFYKNEIDAMSIYQIAYRLKESVKQTRNKNHMKEIAYVTPHGIEFNPEI
                     FKHYPSYNVDTDVVSSNLTHFNDLESLGIGSDIGRFVHVDSTVQTSFIILKEKTGRIF
                     AQLTSRYPFNNSCLFCSND"
     gene            3951..4964
                     /locus_tag="PLR13_06795"
     CDS             3951..4964
                     /locus_tag="PLR13_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQC19082.1"
                     /translation="MKVLVTGGGGFLGGFIVRQLLDRGDNVRSFSRGFYNDLRKLGVE
                     QCQGPFDDLKAVSEAVAGCNAVIHVGGKVGSWGAYEDYYRANVTGTENVINACMKHGV
                     KRLVYTSSPSAIFNRNNMIGVDESVPYPKHATHYSKTKAIAERLMIGANGNNLRTVSL
                     RPRLIWGPGDNHFAPHLVARAKAGNLLLVGNGKNLIDTVWVENAARAHLLALDRLQPG
                     AKIAGKVYFITNGDPRPIEFIINMILDVYGLPPVKHHVPRWLAYAGGWFFEKTFDKLN
                     LQGEPLVTSFMVDELTKSQWFNISAARNDLEYKPVTTIEDGARLYKEHMSKGFSFYGG
                     GSI"
     gene            5508..6260
                     /locus_tag="PLR13_06800"
     CDS             5508..6260
                     /locus_tag="PLR13_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19083.1"
                     /translation="MSEAPIVFVAGWVSAVSGWMPLLKVMASRRPVYYIETREKRSAL
                     IEKRKPKPDDFSIKRMAEDLISVCHQLKIATPTTTVVGSSLGATALLEALKGGHLTAG
                     TAFLIAPNSDFKAPWYLQWILFLPAFLYHGIKYFVLWYLRTFMVDVKKEPEQMMRYND
                     TLRVAHPQRFKLSARSAIYDHYEVWPNLSSVTIPVALAYAPTDTFHSSDNIRLLADRL
                     PKATVVSCPSNKYMHSAEVMQDIEAFVFKSAS"
     gene            complement(6413..6832)
                     /locus_tag="PLR13_06805"
     CDS             complement(6413..6832)
                     /locus_tag="PLR13_06805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQC19084.1"
                     /translation="MNQKDRETILKKAQNEPFAKKFQLTFVDLQKGYAKVAMVFTPDM
                     ENIFGMAHGGAIFSLMDAAFEIASNSHGTMAVALNMSINYLASPEKGATLTAEAREIN
                     RTKKTAAYDIRATDDSGKLLASCQALVYRLEKPLPFL"
     gene            complement(7437..8597)
                     /locus_tag="PLR13_06810"
     CDS             complement(7437..8597)
                     /locus_tag="PLR13_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQC19085.1"
                     /translation="MYRIDLLNKISEKGLSLFSDQYEYRTDIPDPDGILVRSKEMKDM
                     TLPLSLKAIARAGAGVNNIPIDKCSEKGIVVFNTPGANANGVKELVILGMLMACRNVV
                     GGIAWAKGLVGEGDKVPDLIEKGKSKFAGTEIMGKKLGVVGLGAIGTMVANTAEGLGM
                     EVWGYDPFLSIEAAWRLSRNTKKAASLDKLLAECDFITLHVPQNKDTKGFMNTEKFAV
                     MKKGAVLLNFARGGLVDTAALKKAIADGIVGCYVTDFPDEEVIKTDKVIPIPHLGAST
                     EESEENCAVMAAMQLMDFLENGNIKNSVNFPECALDKSGKKRITIANQNTPGMIEKIT
                     HLLADNKLNIGDMINKSRGNLAYNIIDLEGDISEDLINKITSIEGIIAVRVI"
     gene            complement(9252..9326)
                     /locus_tag="PLR13_06815"
     tRNA            complement(9252..9326)
                     /locus_tag="PLR13_06815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9292..9294),aa:Lys,seq:ttt)
     gene            complement(9392..10696)
                     /locus_tag="PLR13_06820"
     CDS             complement(9392..10696)
                     /locus_tag="PLR13_06820"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQC19086.1"
                     /translation="MANVAIVGTQWGDEGKGKIVDLYTEKADIIARFQGGNNAGHTLV
                     VKGKKTILHLIPSGILHDNKICVIGNGVVFDPVVFLEELEQLRQGNLLPANTKLFISE
                     KAHLIMPYHRSIDQAREARHSGKKIGTTGRGIGPAYEDKVARTGIRVGDLYEEDLFRE
                     KLHISMEEKNFLLANFFHDKPLDKQEIAAQYLAYAEKLKPFVADVSLILDREIKKGRK
                     ILFEGAQGCHLDIDHGTYPYVTSSNTVSANASCGTGIGPNAINKVVGICKAYTTRVGE
                     GPFPTELNDQVGEHLQQVGQEFGATTGRKRRCGWLDMVLVRQAVRVSGINALAITKLD
                     VLTGLDKIKICVGYKSPSGEYTYAAPASLKALAECQPVYEEFDGWNEDILYAKEISEL
                     PANTRKYLKRLEELAEAEITLISVGPGREETIVMEDPFLNRI"
     gene            complement(10810..11937)
                     /locus_tag="PLR13_06825"
     CDS             complement(10810..11937)
                     /locus_tag="PLR13_06825"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HQC19087.1"
                     /translation="MQNPNFSSGPCSKRPAWSIDALKGAPVGRSHRSALGKERIVKAI
                     NDTRKVLNIPADYLVGILPASDTGAFECAMWSLLGPKPVTVLVWESFSNGWATDVNKQ
                     LKLHATVIKADYGKIPDLSKVDWKTDVVFVANGTTSGVRIPDWNWIPADREGLSICDA
                     TSYAFANEVDWSKIDVLTFSWQKCLGGEAAHGMMILSPKAVARLESYDPPWPMPKIFR
                     LKKGDKISKAIFDGDVINTPSMLCVEDYLDALDWAGKIGIDGLIKKSKANLKVVEDWV
                     AKTDWIEFLADDPKVRSNTSVCLNVIHPKVAAMGPDEKSKFLKSITSDLSKKNIAHDI
                     NSYKEAPAGYRFWCGPTVEESDLKVALAELEKVFNEKVKNL"
     gene            complement(12208..13686)
                     /locus_tag="PLR13_06830"
     CDS             complement(12208..13686)
                     /locus_tag="PLR13_06830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQC19088.1"
                     /translation="MENNMETDSKTIYPQDVLPEISIEELTPEMRDACDRAGWKTLVP
                     VQAKSIPYFLAGRDMMVQSRTGSGKTGAYILPILQKINAQQNAAQALILVPTRELALQ
                     VSREAEMLTRETRIRIAVVYGGVGYNAQLEAFRGGAQLVIGTPGRILDHLLKNTLSLD
                     HLRILIFDEADRMLSMGFYPDMVKIRKYIQSDKILSSMFSATFPPQVIRLADQFLHHA
                     ELLSLSGSQVHIAEIEHIYYVVPGMRKERSLVRIIEIENPSSAIIFCNTKENVHYLSV
                     VLKRFGYDADELSSDLAQSMREKVMARVRRGALRFLVATDVAARGIDIPDLSHVIQYE
                     PPEDPEAYIHRAGRTGRMGSSGVAISLVAEMEKFKLQSIARYYNINMEERPLPSEDEL
                     ERVVSERITALLEAKLRARDNLQIERMRRFLPLARSLAQAEDELSLITMLLDDYYQQS
                     LHAPPAPAAEVVDQAKGHTQTDKSKKPRSRNKNRKKRQENLS"
     gene            complement(14086..14739)
                     /locus_tag="PLR13_06835"
     CDS             complement(14086..14739)
                     /locus_tag="PLR13_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19089.1"
                     /translation="MARIRNTLLDNIRPLQSDWAVLLDTDIYFTEEILADMFALSPSQ
                     KGIGMLCAFGMEGLPERKGTGGITRLGMFFANGMKGFPKSTGGGWITQSHYYDTWAFL
                     DEDNRFYWPHCIFSGCGKCSAKNAKDKLNAAGVVNVNSAFGGFAIVKSQLLNHPDLRW
                     KAVPSEDSDIRGSAASERKMMLCEHIHFCKMLRKVSSLSVAIACDIPVYWDLRSYRS"
     gene            complement(15143..15439)
                     /locus_tag="PLR13_06840"
     CDS             complement(15143..15439)
                     /locus_tag="PLR13_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19090.1"
                     /translation="MFEDFDSVKKNKKYRFTCQGNCDNPHKTLADFPLQMCGRLGKYD
                     VFIHHHILCVTEYDCSAGNIKEQPTWHEINLYEPDPDHKGAMRTNGFKKSVQGA"
     gene            15475..16479
                     /locus_tag="PLR13_06845"
     CDS             15475..16479
                     /locus_tag="PLR13_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HQC19091.1"
                     /translation="MLVRLVKDWSWPDLMRQTPQEDGKWRDIQFTFEPVKHCDYLIML
                     NNKRDIPVSVRCPPEHVWCIMQEPYIPFSNDWMLKDLDWCSRILTHYPAGLSRAEESH
                     PAVPWHVDLNYKQLMWGRRPVKQRLLSWITSRLTVTPMQRKRMAFYEYLQSCKVPVDV
                     YGRGIRHIRDKWDGLAPYYYSLAIENDRSSDYWTEKLADCFLSWTVPVYDGCLNLEKY
                     FPPESFIRINIDDPDASLKIIAETSEPDDWRRRLPALQEARELVLKRWQFFPFIYEKI
                     KTDLRQAGPASEISIPDNRIMRLTDEFRFLMDELKQRGPLALGRQLNNKIKYFHWRNY
                     "
     gene            complement(16687..18144)
                     /locus_tag="PLR13_06850"
     CDS             complement(16687..18144)
                     /locus_tag="PLR13_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19092.1"
                     /translation="MRPVVLLRPAEGLYAKVFRPWVPLSLLAAASKLDEAGYPIILID
                     QRISVDWKSDLQKALSQNPICVGVTSMTGSQITHALEMSAFVKQHSAVPVIWGGVHAT
                     LFPLQTVEHSLVDIVVKGEGEETFLLLALRLEQKQSLHGLEGVVYKDRGDIVNNPDRP
                     FLDLNDLPPVPYHLIDVNKYLHRYFDEDRVLEFETSRGCPFNCTFCYNPLYSRRRWRA
                     LSASRILEFMQPLVETHGVKAFHFVDDGFFIDHERTREIMRGILNRKWKIKMGFQGAR
                     IDTVDDLQDEDLELITKAGGTFLQVGVESGSPRILDILNKKIHPEQVLAFNRRLSRHP
                     HMKVYYNFMCGFPTETAQDVLKTAALAWTLLRDNPYALISAFHFYKPYPGTSLRERVI
                     HKDDVTPSTLEGWGNFNWTQYMGRDQDADTKALMEKIEIVSILADRKMSYQSDSAALR
                     MLAAVYRPLARFRLRHGWYSWMPEHWLRKWLGDRT"
     gene            complement(18450..19685)
                     /locus_tag="PLR13_06855"
     CDS             complement(18450..19685)
                     /locus_tag="PLR13_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19093.1"
                     /translation="MKNREIRAYLENNAGKNILPLSIIENEELIGSKPIEIIVFYKKK
                     KAISELKECLLQTIEYYNLFSSRLIKIGHHQYALQFASDGFTVNILPPVEAPVAKLPI
                     DDIRQSMVHVKTLPGEPLFAVTGFSTQDGTVGAVSCSHAVADGISFFLFLFAWVSIID
                     GNPFSRPSKQRLFTGSPLNSGHIDKMFKPSLSKLSNVIQNRIHYENNIKTYRHTDYFT
                     CEFLNDIKKHAKRGNGDDCISNNQIINAVLMKKYHQHLLPDTRKIVMRNPVNLRDVHP
                     DIDPLYIGNAYLDCTTEFTKDEVENLSVYDIARRLKESIHNAKNENFVRDMAYLSKYG
                     IEFKSDIFNHYSPDNIKTDIVSSNIAHLHDLESMGMGSNILDILHVDSAVQTGFVILK
                     ENSRLILVQTTGMYPFESR"
     gene            19828..20379
                     /locus_tag="PLR13_06860"
     CDS             19828..20379
                     /locus_tag="PLR13_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19094.1"
                     /translation="MTDQNDMVDYVIEPLTFKYLLPALRLVDSVFPRREQGGERADLA
                     FTASLLTLGRRIAKWMGYPMVRYWIVRDVATNRIYGTIGHYVRADDPDAYWGGWMCVD
                     PRARGLGIGKKLINFVVGESVRRGDRPYHRFYTSTDPNEAKAVAMYNRACFNVYKQEC
                     DPVTGYTRLYYQVRLDDVTLPVL"
     gene            20424..21179
                     /locus_tag="PLR13_06865"
     CDS             20424..21179
                     /locus_tag="PLR13_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HQC19095.1"
                     /translation="MRKKVVLFSGESVFGSTIINRLLNKQSFDLIGVFYSTRVYKKSS
                     SIFSFYQVLSITGMQYYWNIVKLNAPFAKLFYPEVTRTGKVCRQNGIPIFYASDVHWP
                     ANIEIITQLKPDIILSAYFNQLIKEPILSFPAEVLNIHPGILPEYRGLDPLLQQIIHG
                     EDHLGMTLHRVDETFDTGEILRQKTMLRDKTKSLFELYMEIWEQGVDMLEDYIRFGKQ
                     PIENLTERQQRSSYFSWPTKKDVQIFLEKNRFF"
     gene            complement(21452..>22696)
                     /locus_tag="PLR13_06870"
     CDS             complement(21452..>22696)
                     /locus_tag="PLR13_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19096.1"
                     /translation="MFEYIGYTCAFLAIVSSFIEFTMALTLRSHVKNWSRHKPGKYPA
                     LTVIAPQRGKIVPENIEALLAQQYPGTWEVIFVTTEDDASLPLLKNYSNKHENVRIVI
                     ADDVVQLSKNQCIHRTQKNSNVVAAINSSSPKTEVYVIVDADARPFMDWLRNLAAPFT
                     DGHSKLGAVTSARLYLPGKGLASWVQALWIINSAPFLAGKHGYIWGGGLAISKAVFEK
                     ANLISSINGQGKHPITSEDNNINNALRTQGYETVFVPNCIVPRYPPDRKEKWRDVISF
                     TNRQVLQFFWTNRSRWLLSSLMTKIRMPMFFCAMVVAWWYPLSSLALLSPLIDMTYSF
                     IIMTALLTKAQREALNIKLNLWAVFLPMLVQILNTINFLSMPYYRSMKWSGIEYTRRK
                     VVGYTGNFSWREQEFIKSDKNS"
     assembly_gap    22697..22796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22797..23659)
                     /locus_tag="PLR13_06875"
     CDS             complement(<22797..23659)
                     /locus_tag="PLR13_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19097.1"
                     /translation="MNRTRGFMDLSVYRDLLDEIGEYLFFLFFWDWGEPFIHPSAFDM
                     IAYAHEKKIRIISSTNGHLFADQHAADEAIRCGLDTLIFAVDGISQTTYEKYRHKGDL
                     QKSVDGLRMLVERKKVLRSSTPLINFRFIVMKHNEHEIERLPSFVRKLGANVLTLKTL
                     NPLADNTYGDKPDGIKNYDLVPQNPRYSRFIFDENGRPVRRKRNACRNLYNSSTIHWN
                     GTVCPCTYDYDDRFVLGNLHNNSFEDIWEGSAYRAFRDRFRRNDPKNLFCYECSYAFK
                     GGSCIDETVLPA"
BASE COUNT         6364 a         5340 c         5679 g         6669 t
ORIGIN      
        1 gcgaaggaat gatttatagt aggacgaacg tcctattgtc aagaaagaaa taatgtctat
       61 ttcatgaaca aataaaaata tcagtttgac agttacagtc gatttagata aagactgata
      121 gtcagaaaat aggctttgtt aataaaacag gcgacatttt aaaagacgca gagattctgc
      181 aaaaaaatat gagaagagga attcaatatg gcacacagta attggttgta tcagacacgg
      241 gacatcatgt tccagatcaa agaatggctc ggcgtggaaa agctcttgag ccttgatgct
      301 tacaaggact actacggaat ggacgacatc aacagcatcc tcgatgtcaa ctataaagtc
      361 tgccgcgacg tgatgtgtcc ggccaacaaa gacgccgatg agcccggcgc aaaatttgtg
      421 ggcggcaatg aacatgccgt ggtgactccg gactcattca agaatgttta taaaaccgtg
      481 atggatgcgg agctgggacc ccagttcggc taccgcggcg aaggcaaaat accgctgtgc
      541 tggtatgcgc cgattctgga gatgcagtcc gccgcgtcgc cgtcgatcgt catgttctgg
      601 tgcctgacgc agggcgcgat caccgtgctc caggaatttg ccacgcaaaa acagcaggac
      661 ctgttcatcc ccaagctgcg gaccggcgaa tggggcggga ccatggggct taccgaaccg
      721 ggcgcaggtt ccgaagtcgg cgcctgcaag agccgggcaa ttcccaccga tgtccccggc
      781 aaatataagc tcacggggac gaaatgcttc atctcctccg gagaccatga cctgacggaa
      841 aacatcatcc atttgatgct ggccaagacc ccgggcgcca aggaaggcac cgcgggcata
      901 tcgcttttca tcgtgcccaa gttctgggtc aacgacgacg gctcgctggg cgcctggaat
      961 gacgtcacca ccatgggcat cgagcacaag ctgggcattc acggctcgac gaccgccacc
     1021 ctgtccatgg gggaaaacga caactgctac ggctggatgg tgggagaagc ggaagtgaaa
     1081 gatggcagag gcgttgggat gaagcagatg tttgcctaca tgaacgaaga acgtctgaac
     1141 accggattgt tttccctggg atgcatgggc tccgcctact acgcagcgct ggagtatgcc
     1201 aagatccggg tgcagagcaa acattccacc aaccccaagg gtcccagcgt ccggatcatc
     1261 gagcacgaag acgtccgccg catgctgatg tttcagaaat ccatgataga ggcctgccgg
     1321 gcgttgattt acaagtccta tctctaccgt gatctggcag tagacgccgc cacgccggaa
     1381 gagagagact attacgatga catgtttgcg atcaacaacc ccctgtgcaa ggcctataca
     1441 tccgacatgg ccaggctcac caccggggac gccatccagt gtcacggcgg ttacggcttt
     1501 acggaagagt atgccccggc ggaactgtac cgggacgttg tgatctatgg aatttgggaa
     1561 ggcaccaact tcatccaggc acaggactat accggccgca agttcaccat gaaggacagc
     1621 gtgccgttta cgaagtgggt ggcggaaatc gacgcctttg tcgtcggcaa gaagaccgac
     1681 gaatttgcgg cggaattcgc gatgatggcg gatgccatgg tggcttttaa agatatcgtt
     1741 gaaatgaatg ctgcctggac aaaaggcgat aagcagctga aacagcttta cgccacccgg
     1801 accatgcatg ccggcgcaag agtttactgc ggtatgctgc ttttggatca ggcgctcctg
     1861 gcggcccgga tgctcaaaga gaaaggcgac gtcgatgcgg atttctacaa ggggaaaatc
     1921 gccaccgcca ggttctatgt catgaatcat gttcccgata tcttcggata tctgaaaacg
     1981 atgaagtgcg gcgatcgcag cgcgatcgac attcccgaag aaagcttcat gtagggcgaa
     2041 cgcaccctgc ttgcgggcgt aaagaaaaga aggttgtcgg gcatgagata atataaatta
     2101 agttcaaaac gggatttgcg gaaaggcagg atcaaatgat cctgccttta tttttattta
     2161 ttccttgctt cctgcaagac acatttttaa aatttatatt tttatgccag gtttcaacca
     2221 gtaatcatga cctaaacaaa tatccttctt tcgagggatt gacaaaccaa tttaagagta
     2281 tgttttaaaa atctttaagt cacaatcaga agcattttga aatcataact taatggggga
     2341 tggatgaaga aacatctctg gattccgcag ttcatttgta ttttgatgct tctattggcg
     2401 ttaaatccaa gcaatccgta cgcatattac acgctatttc gttgggtttg ttgtggcgta
     2461 tttgcctatc ttgcatttca atcatttgag cagaataaac aagagttatt aaaaggatac
     2521 ctgctcgtca gctgagcgaa tattctgccg gttttttctt tgaggatgat aaaacttgtt
     2581 tgaacggttg aatcaacatg aacaaacctt ccgatatccg aacctatacc cagagattcg
     2641 aggtcattaa aatgagtcaa attgctggaa acgacatcgg tatctacatt gtatgaaggg
     2701 taatgtttaa atatctcagg gttgaattct atcccatgcg gagttacgta tgcaatttct
     2761 ttcatgtgat ttttatttct tgtctgcttg actgattctt ttaaacgata ggcaatttga
     2821 tagattgaca ttgcatcgat ttcgtttttg taaaattctg taaaactatc gaaaaccaca
     2881 ttgccgatat ataagggatc gatatctgga tgtatccctc tgaaatcgac aggaattttt
     2941 attttaattt tgtcggcatc aggcatcatc aaatgatgat attttttcaa taagaatgac
     3001 gtgatgattt gattattgga aataatatat tttttatttt cctcctttgc tttctttttg
     3061 gtttcgttga gaaatgcatc cggaaaatat tcccttttaa agtatcgttt ggcattgatg
     3121 gcgtggactc gcttttgaat tttttcattc agctcggaca atggcggtat aaacgatttg
     3181 tccattttgt caaagcaaac cggattgccc cggaataatc gttgcgtcga tggaagagga
     3241 tagctcttgc cctgtaatgc gcatccccaa gcatataaaa aaagcatcag ggaaattccg
     3301 tcggctattg tatgggaaca gctgacgccg ccaaatatgc catcgtttac cggtattccg
     3361 gtcacggcaa aaagtggctc tcccggtaat gttttaacgt gcgccatcct gttctttata
     3421 tcgtcaatct caacctcatc agacgcagcg atgatgggcg gcaggatatt gaattgaacg
     3481 ccgtccgtac aatagagtaa tgcaaattta ttcttgtctg tcatgatcag acgacttgag
     3541 aaaaggttgt aatgctcgat ggctttgaaa agacactctt tgaattttga tagaggattt
     3601 ttttctttat agaacagcac aagctccacc ggtcttccac cggggaatcg gtcattatcc
     3661 actagtgata gtggcaaaat attttttccg taattttctt caagggatat catctttttt
     3721 tgagcattca ttaaaatcat tccgggatgg agataaaaaa tctactttat aacggaggga
     3781 gcatattcag aactattttg cgtgtcaatg caattttcag acgcgaaata aaaaatgaca
     3841 gaattttcaa acggcatgtg atacagaaac atggtaaaga ttcctttcca atacacggaa
     3901 tcagccaggg tttttgaaaa acagtttggc cggaggagca aagaaatttc atgaaggttc
     3961 tggtaacagg gggaggcgga tttttaggcg gttttattgt ccgccaattg cttgaccgtg
     4021 gtgataatgt gcgaagtttt tcccggggat tttataatga tttgagaaag cttggcgtag
     4081 aacagtgcca agggcctttt gacgatctga aggccgtctc cgaagctgtc gcaggatgca
     4141 atgccgtcat tcacgttggc ggcaaggtcg gatcttgggg ggcctatgaa gattattaca
     4201 gagccaatgt aaccggcacc gagaatgtga ttaacgcatg tatgaaacat ggtgtaaaac
     4261 ggctggtcta cacatcgtca cccagcgcga tttttaaccg gaataacatg ataggtgttg
     4321 acgagtctgt tccgtatccg aagcatgcca cccattattc caaaacaaag gcaatcgccg
     4381 aacgtttgat gattggcgcg aacggaaata acctgcgcac ggtttcactc cgtccgcgtt
     4441 taatctgggg gccgggagat aatcattttg ctccacacct cgttgccagg gccaaagcgg
     4501 ggaaccttct gctggttgga aacggtaaaa atcttattga caccgtatgg gttgagaatg
     4561 cagctagagc gcatctcctc gcccttgaca ggctgcaacc aggtgccaag attgccggca
     4621 aggtttattt catcacaaac ggcgatccga gaccaatcga attcatcata aacatgatct
     4681 tggacgtata tggactgccg ccggtaaaac atcatgtacc caggtggctc gcttacgcgg
     4741 gcggatggtt ttttgaaaaa acttttgata aattgaacct tcagggtgaa cctcttgtaa
     4801 cgtcatttat ggtagatgaa ctgaccaagt cacaatggtt caacatatca gcggcaagaa
     4861 acgatctgga atacaagcct gtaaccacca ttgaggacgg agcgagactt tacaaggaac
     4921 atatgtcgaa gggtttttct ttttatggag ggggtagtat ttgaaggcct ctgatacctc
     4981 cgcaggccag aagcggggat tgctttactg aattaatttt ccaataaagt taaaactgca
     5041 atgacctgcc ccaagacttt gtaccacaag tcaagttaga aatccgttat tttttgttgt
     5101 tccatttata tacgcttccg gtgcaggggc gttagttcaa tgtactgtac agcctgaatg
     5161 tgttgtattg aagcctccat tgttcagtca gcttccgggc ctcattactg accctgcctt
     5221 tcttacgcag attataaatg gacataatta aattttgcgg acaagaaaaa ttgtatttta
     5281 gtaggtaacg gcaagattct tcctgaaaat aagtgtccgc tattttcaat agacattcaa
     5341 ccttccaata tgtaataaat acaaatatct tctccataag ggggattaat gtgaaagact
     5401 ccatagcgga tccggtggca ttaccggatt cagtttttag caggttgtca gtttgcgtgc
     5461 agagcggtgt ccgccttcgt gtcatgacct ggcgacccca aaaacccgtg agtgaagctc
     5521 ccattgtttt tgttgcggga tgggtttcag ccgtatccgg atggatgccc ttgttgaagg
     5581 taatggcttc cagaaggccg gtttattata tcgaaacacg ggaaaagcga tcagcactca
     5641 ttgaaaaaag gaaaccgaag ccggatgatt tcagtatcaa gcggatggcg gaagacctga
     5701 tcagtgtttg tcaccaactg aaaatcgcca caccaaccac caccgtcgtc ggcagttccc
     5761 tgggggcgac agcactgttg gaggcgctaa aaggggggca cctgaccgcg ggaacagcat
     5821 tcctgattgc acccaatagt gatttcaaag ccccatggta tttgcagtgg attctttttc
     5881 taccggcttt tctatatcat gggattaagt attttgttct ctggtattta aggaccttca
     5941 tggtggatgt taaaaaagaa cccgaacaga tgatgcgtta taacgatacg ctgcgggtag
     6001 cccatccgca gagatttaag ctgtcagcca gatcagccat ttatgatcat tacgaagtct
     6061 ggccaaactt gagcagcgtg accatacctg tcgctttggc ttatgcccct acggatacgt
     6121 ttcactcatc ggacaatatc agactcctgg cggacagatt acccaaggca accgtcgttt
     6181 cctgcccgtc aaacaaatat atgcatagcg cggaggtgat gcaggatatt gaggcgtttg
     6241 tcttcaaatc ggcatcataa ctctggggaa aaatggacag ataataaggt ttattgtgct
     6301 caatgaaggc ttataatgtt aactttcaag cggttatcat tgatatttag tgatgcaaat
     6361 aagtatgaca tttttaggta aaatcagcga ccacttcata aataattgcc gcttataaaa
     6421 acggcagcgg cttttccagc cgataaacca gcgcctgaca ggacgccaga agctttcccg
     6481 aatcgtcggt tgcgcgtata tcgtaggcgg cggttttttt cgtcctgtta atttctctgg
     6541 cttcggcggt cagcgtagcg cctttttccg gcgatgccag ataattgatg ctcatgttaa
     6601 gggcaactgc catggtaccg tgtgaattgg aggctatttc aaaagccgca tccataagcg
     6661 aaaaaatagc gccgccgtga gccatcccga aaatattttc catgtccggt gtaaaaacca
     6721 ttgccacttt tgcgtatcct ttttgcagat cgacgaacgt taactggaat tttttggcga
     6781 aaggttcgtt ctgcgctttt tttaaaatag tctccctgtc tttttgattc atgagtatta
     6841 ctcctgcatg tttttctgga caattgatac gttcttgtaa cgctataata aaaagggggg
     6901 taatctgtcc agctgaaaac aaaaacgctc aattttgatc ttgcttttgt ggtacaggct
     6961 gcaatattcc gactactacc tatagtagtg caaaaatacg attaccacca catattgatt
     7021 tttttcttgc tttttcaaaa aaatattata aaataactga aagtttgaaa gaaaatcttt
     7081 tggcagagaa attgaggttt cggtttggaa ggttctttga agcaaacggt ttttagatgg
     7141 caaaatgcca aagtttgttt aaactggaga gggaccgctt ctgacgggga attttcgatg
     7201 tatcgtcggg gagatagcaa aacagaacat gatcatatgg caaacgccga atgaagcggt
     7261 tgcaaatggt taaccaaatt ttaaaccctt gcctgaaggg cggatcgtaa aagatccgcc
     7321 ctttttcttt cgttaaatgt tcaggcaagt taaaatccgc cattgctgta tcgaaaaaaa
     7381 agtgtctgac gaaggccgcc agacactttt tgatatgaca cgtgacagat gttttcttag
     7441 atgactctaa cagcgatgat tccttcaatg gacgtgattt tatttatcag atcttcgctg
     7501 atatcacctt ccaggtcgat gatgttgtac gccagattcc ctctgctctt gttgatcatg
     7561 tcgccgatgt tgagcttgtt atcggccagc agatgcgtga tcttctcaat catgcccggc
     7621 gtattctgat tggcaatggt aattcttttc ttgcccgatt tgtccagggc gcactcaggg
     7681 aaatttaccg agtttttgat gttgccgttc tccaggaaat ccatgagctg catggcagcc
     7741 atcacggcgc agttttcttc cgattcttcg gtggaggccc ccagatgcgg aatggggatg
     7801 actttgtctg ttttgataac ttcttcatca gggaaatcgg taacgtaaca gcccacaata
     7861 ccatcggcaa tcgccttctt cagggccgct gtgtccacga gcccgcctct ggcaaaattc
     7921 aggaggacgg cgcctttttt catgacagcg aacttttcgg tgttcataaa tcctttggta
     7981 tctttgtttt gcggaacgtg cagcgtgatg aaatcgcact cagccagaag cttgtcgaga
     8041 ctggctgcct ttttggtgtt gcgggagagc ctccaggccg cttcaatgga gaggaacggg
     8101 tcatatcccc agacttccat gcccaagcct tcggcggtat tggcgaccat cgtgccgata
     8161 gcgcccagac cgaccacgcc cagtttcttg cccataattt cagttccggc aaacttggat
     8221 ttgcccttct cgataagatc aggcactttg tcgccttcgc caaccagacc tttagcccag
     8281 gcgattcccc cgacaacatt gcggcaggcc atcagcatgc ccaggatgac cagttccttc
     8341 acgccgttgg cgttggcccc cggtgtatta aaaacgacaa tgcctttctc tgaacatttg
     8401 tcgatgggaa tattgttgac gccggcgccg gcgcgggcga ttgctttcaa tgataacggc
     8461 agggtcatat ctttcatttc tttacttctc accaggatgc cgtccggatc gggaatatca
     8521 gtgcgatact cgtactgatc tgaaaaaagg cttaacccct tttcagaaat cttgtttaac
     8581 agatcgatgc gatacataaa tacctcctat tcctttctaa tgagacccaa atttcagaaa
     8641 cgatgtccac tgaaacttgt aggtcgaatt tttccgaggc gaagcatggc gggacattga
     8701 gccatcccga acagcgtaga tgatgaacct caaaatttgc ttcggggttc atacccgtga
     8761 ataaggagtt ctggcaggaa aactcctgag ctttgtcgcc atagtacaat acagattgaa
     8821 tttatctcat ggatacgaaa ccatctgttt tgcaggacga gtatagaatg ggtgctttta
     8881 cgtgtcaagg aaatcttaat gagatccatt gagagcaaac gaagtgatgc tcgaaatgcg
     8941 agggatactg tccaataacc ggagtttatt ggacagtatc ccccggtgca tagcgccgtg
     9001 gaaactgtgt ttacggtaag gaaatatgaa ttttaacgag acccagattt cagaaacgaa
     9061 gtgcaccgaa atttgatggt cgaattatcc ccgaggcgaa gccgagcggg ataaatgtgg
     9121 gtctaattcc ccgcccccta gagcgcagtt cctttggata ccatgctgct gtcggcgggg
     9181 ttgtttatca tatcaaatga gtcttggtag atgtaaaaaa aggttccctt agggggaacc
     9241 cttatttttc ctggtgggct gtgcggggat tgaacccgcg gccaatggat taaaagtcca
     9301 ctgctctacc gctgagctaa cagccctttg tctgcaattc taaaagcgtg ccctcttaac
     9361 aactagttgg cactgtgtca agaaaaaact gctaaatcct gttcagaaaa ggatcttcca
     9421 taacgatggt ttcttctctg cccggcccga ccgatatgag tgtaatttcc gcttctgcca
     9481 gttcttccag ccgctttaaa tattttcggg tgttggccgg caattcactt atttccttgg
     9541 cgtaaaggat gtcttcattc cagccatcga attcttcgta aaccggctga cattcagcaa
     9601 gagcttttaa actggccggt gcggcatatg tatattcgcc ggacggcgat ttatatccga
     9661 cgcatatttt tattttatcc aggccggtta aaacgtccag ttttgtgatg gcaagagcat
     9721 ttatgccgga aacacgtact gcctgtcgta ccaggaccat gtctagccag ccgcaacggc
     9781 gttttctccc ggtggtggcg ccgaactcct gtccgacctg ctgcagatgc tcgccaacct
     9841 gatcgttcaa ttccgtggga aaagggcctt ccccgacacg ggtggtgtag gctttacaga
     9901 taccgactac tttattgatc gcattgggac cgattcccgt cccacatgaa gcattggcgg
     9961 agaccgtgtt ggacgatgtt acataaggat acgttccgtg atcgatatcg agatggcatc
    10021 cctgagcacc ctcaaaaaga attttcctcc ccttcttgat ttcgcgatcg agaatcaggg
    10081 aaacatccgc cacgaacggc ttgagttttt cagcataggc caggtactga gctgcgattt
    10141 cttgtttgtc caacggtttg tcatgaaaaa aattggccag aagaaaattc ttttcttcca
    10201 tgcttatgtg cagtttttct ctgaacaaat cttcttcata gaggtcaccc acgcggatac
    10261 ccgtgcgggc aactttatcc tcataggcgg gaccgatgcc gcggccggtc gttcctattt
    10321 ttttgcccga atgacgggct tctcgtgcct gatcgatact ccggtgatag ggcatgatga
    10381 gatgggcttt ctcactgata aacagctttg tgttggccgg taacagattg ccctgtcgta
    10441 actgttccag ttcttcgaga aacacgaccg gatcaaacac cacgccgttg ccgatgacgc
    10501 aaattttatt atcatgcaga attcccgagg ggatcagatg cagaattgtt tttttgccct
    10561 tgaccaccaa agtatggccg gcattattgc cgccctgaaa ccgggcgatg atatcggctt
    10621 tttcggtgta aagatcaaca atctttccct tgccctcgtc tccccactga gtgcctacga
    10681 tagcaacatt ggccatgatt tatttcctac gtttaatggg ttggatagaa ataacatacg
    10741 gcagctctgg cagtatcatg atgcaatagc ttcccgctgc cgtatggata tttaaatctc
    10801 gtcaatcaat taaaggtttt taaccttttc gttaaaaacc ttttccagtt cggcaagtgc
    10861 caccttgaga tcagactctt caaccgtcgg gccgcaccag aaacggtaac ccgccggcgc
    10921 ttccttgtat gaattgatat cgtgagcaat atttttctta ctcagatcgg aagtgatgct
    10981 tttcaggaac ttgctctttt catcgggacc cattgccgct actttaggat ggatcacatt
    11041 caggcagaca gacgtgttgg agcgtacttt gggatcatcg gccagaaatt caatccagtc
    11101 ggttttggct acccaatctt cgacaacttt caaattggcc ttgcttttct tgatcagacc
    11161 gtcaatgccg attttacctg cccaatcaag agcgtccagg taatcttcca cgcaaagcat
    11221 ggaaggcgtg ttgatgacat cgccgtcaaa gatcgctttg ctgattttgt cgcctttttt
    11281 cagtcggaaa attttaggca tcggccacgg aggatcgtag gactcgagac gggcgacggc
    11341 tttgggacta agaatcatca ttccgtgcgc ggcttcaccg cctaggcact tctgccagga
    11401 gaaggtcaga acgtcaattt tactccagtc aacctcattg gcaaaggcat aactggttgc
    11461 gtcgcaaatc gacagacctt cacggtcggc cggaatccag ttccagtcgg gaatcctcac
    11521 gccgctggtt gtgccgttgg cgacaaaaac cacatccgtt ttccagtcca ctttggaaag
    11581 atcgggaatc ttgccgtaat ctgccttgat aacggttgca tggagcttga gttgcttatt
    11641 gacgtcggtt gcccagccgt tggaaaaact ttcccagacc agaacggtaa ccggtttagg
    11701 acccagaagt gaccacatgg cgcattcaaa agcgccggta tctgaagccg gaagaatacc
    11761 cacaaggtaa tctgccggaa tgttgagaac ttttcgtgtg tcattgatgg ctttaacgat
    11821 tctttctttg cccagtgcgg aacggtgtga gcggccaacc ggcgcgccct tcagcgcgtc
    11881 gatactccac gctggtcttt tgctgcatgg accggaacta aagtttgggt tttgcataat
    11941 aaacatcctt aagataaatt ttaaaaaact aattatttat atcagcattg aaatctttac
    12001 tcaagaaatt ttagcttatt ttttaccggg ggaaacatct tgcgccgatg agaatttcca
    12061 aaatagctca attggcaaaa aataggtacc gtccggtccg atatgttttt ctcggggaaa
    12121 atgttaattg atctggatat ttagaaataa taagccctgg gaatcataaa gccggaaaat
    12181 ccggggaagg accaaatcac ttggacttta cgaaaggttt tcttgccttt ttttcctgtt
    12241 tttatttctt gaacggggtt ttttgctttt atccgtttgt gtatggccct tggcctgatc
    12301 aacaacctcg gcggccggag ccggcggagc gtgcaaagat tgttgataat aatcatccag
    12361 gagcatggtg attaatgaaa gttcatcttc tgcctgcgcc agggatcttg ccagaggcaa
    12421 aaaacgccgc attctttcaa tctgcagatt atcgcgggcg cgcagttttg cttccagaag
    12481 cgcggttatt ctttcagata caaccctttc cagctcgtct tccgagggca ggggtcgctc
    12541 ttccatgttg atgttgtagt agcgggcgat gctctgcagt ttgaattttt ccatttctgc
    12601 gaccagggaa atcgccacac cgctcgatcc catccggccg gtgcgtccgg cacggtgaat
    12661 ataggcttcg ggatcttccg gcggttcgta ctgaatgacg tgagacagat cggggatatc
    12721 aattccacgt gcggcgacgt cggttgccac cagaaaacgc aacgcacccc gtcggacacg
    12781 cgccataacc ttttccctca tgctttgggc cagatcggaa ctgagttcat cggcatcata
    12841 accgaatcgt ttaagcacga cagacagata gtggacattt tcttttgtgt tgcagaagat
    12901 aatggccgaa gaaggatttt ctatttcgat gatccggaca agcgatcttt ccttgcgcat
    12961 gccaggcacg acataataaa tgtgttctat ttcggcaatg tggacctgac tgccgctcag
    13021 gctcagcaat tctgcatgat gtaaaaactg atcggccagg cggatgactt gaggaggaaa
    13081 cgttgcggaa aacatactgc tcaatatttt gtcagactgg atatatttac ggattttcac
    13141 catgtcggga taaaaaccca tggacagcat ccggtcggct tcgtcaaata tcagaattct
    13201 cagatgatcc agtgaaagcg tgtttttcaa tagatgatca agaatgcgtc ccggcgtgcc
    13261 gataacgagt tgagcgccgc cgcggaaagc ctccaactgg gcgttgtagc cgacaccccc
    13321 gtaaaccacg gctattctaa tacgcgtttc ccgtgtcagc atttctgctt cccgggagac
    13381 ctgaagcgcg agttcacggg taggcacaag aatcagagcc tgggcagcat tttgctgggc
    13441 gttgattttc tgcaaaatgg gcaggatgta ggcgccggtt ttgccgctgc cggttcgcga
    13501 ctgcaccatc atgtcgcggc ccgctaagaa ataaggaatg gatttggcct gcacgggtac
    13561 caaggttttc catccggcac gatcacaggc gtcgcgcatt tcgggcgtaa gctcttctat
    13621 ggaaatttcc ggaagaacat cctgaggata aatagttttt gaatccgttt ccatattatt
    13681 ttccataagc aaactcacat tgcataaggt ctgtcgaggt cattaacgta tcctcccgaa
    13741 aaggtaacag cgcttatgat tttcaatttc aaaagtgatt tagtgtttat ttcactattc
    13801 tgtaataagc aagaggaaag ttgttcttgc ttatctgccg gtgacagcaa ataaataaac
    13861 atgggggatt ataaagcccg gtggatgact gcagaccgac aaggaaggga ccgtggtgaa
    13921 tgttttgata gaacagaatc agcgcagcag cctcatcttc aagaaactga atattgtcaa
    13981 ggtaaggcaa ccggacaatg atggttatcc caaacatcag tgtcagaata agcaatacaa
    14041 gatttaattt tccatctttt gatggcaatt taatagtcct gattgtcaag aacggtatga
    14101 tctcaagtcc cagtacacag ggatgtcgca ggcaattgcc accgataaac tgctgacttt
    14161 tcgaagcatt ttacagaaat gaatatgctc acataacatc atttttctct cagatgcagc
    14221 acttcctcgg atatcggaat cttctgatgg aacggctttc caccggagat ccggatgatt
    14281 taaaagctgg ctttttacta tggcaaatcc gccgaaagcg ctgttgacgt ttaccacacc
    14341 ggcagcattg agtttgtctt ttgcattttt ggcggaacac ttcccgcagc cggaaaatat
    14401 gcagtggggc cagtagaaac ggttatcttc atcgagaaaa gcccaggtgt cgtaataatg
    14461 ggactgcgtt atccatcctc ctcctgtcga ttttggaaag cccttcatgc cgttggcaaa
    14521 aaacatcccg agtctggtaa ttcctcctgt ccctttgcgt tcgggcagtc cctccatccc
    14581 gaatgcacaa agcattccga tccctttttg cgagggggac agggcaaaca tgtcggccaa
    14641 aatctcttcc gtaaaataga tatccgtgtc cagcaatacc gcccaatcgg attgaagggg
    14701 acgaatatta tcaagcaatg tatttctgat tctggccaat ctttcggttc tgggctgctg
    14761 gtcgagaaaa gatgtgtcct caggcgttat aatctttccg acgcgaccgt ggaggaaatc
    14821 agtcaatagt gtgcgggtat cgtctgaaga tccgttctca gcaatgaaat attcgaaatc
    14881 gcagtcatat gtcttttcaa gtcggatcag tgtgtcaaat aaatgtggaa ggaattcctg
    14941 attgtttctg cataaccaca ggatggaaat tttgcgaacg ggtattcctg attctgacaa
    15001 cgtgattcgc atgaccatcc tgtcgtttga tcggtttcgg gttatgtcaa ttttcgatct
    15061 gtccgatcat cgattgaatt gataaaaaag ctttttttga agacaaagta taaggaacgc
    15121 acttttctgc gtcaataaga ttttaggcgc cctgtacaga ttttttaaaa ccgtttgtcc
    15181 gcattgcgcc tttatgatcc ggatccggtt cataaaggtt gatctcatgc catgtcggct
    15241 gctcttttat attgcctgca gaacagtcat attctgtcac gcagaggatg tgatgatgga
    15301 taaaaacatc atattttccc aaacgcccac acatctgcaa tggaaaatcg gccaaagttt
    15361 tatggggatt gtcgcaatta ccttgacagg tgaaacggta tttcttattc ttttttacag
    15421 aatcgaaatc ttcaaacaat gattgtttta tgaatattca aacggatgaa atccatgctg
    15481 gtacgtctgg ttaaagattg gtcgtggcct gacttgatgc gtcaaacacc gcaggaagac
    15541 ggtaaatggc gcgatattca atttaccttt gaacctgtta agcactgcga ttatctgatc
    15601 atgctcaaca ataaacggga tatccccgtt tcagttcgtt gtccgccgga gcatgtctgg
    15661 tgcattatgc aggagccgta tatacccttt tcgaatgact ggatgctgaa ggatctggac
    15721 tggtgctcaa gaatcttaac gcattacccc gcggggctaa gcagggccga ggaatctcat
    15781 cccgccgttc cctggcatgt tgacctaaac tacaaacaat taatgtgggg caggcggccg
    15841 gtcaagcagc gtctgctttc atggatcacc agccggttga cggttacgcc gatgcagcgt
    15901 aagcgcatgg ccttctatga atatttacaa tcctgtaaag tcccggtgga tgtttatgga
    15961 agaggtatcc gccacatccg tgataaatgg gacggcttgg ctccttatta ttattcgctg
    16021 gcaatcgaaa atgatcgaag cagtgattac tggacggaaa aactggcgga ttgttttctg
    16081 agctggacgg tgccagttta tgacggttgc cttaatctgg aaaaatattt tccaccggag
    16141 tcttttattc gtatcaatat cgatgatcct gatgccagtt tgaaaatcat cgctgaaaca
    16201 tcagagcctg acgattggcg tcgtcgtctg cctgcattgc aggaggcgcg tgaattagtc
    16261 ctgaaacgat ggcaattttt ccccttcatc tatgaaaaaa ttaaaactga tctgcggcag
    16321 gccggtccgg catcagaaat ttccatcccg gataatcgca tcatgcgcct gacggatgag
    16381 tttcgttttc tgatggatga attgaagcaa agaggaccgt tagccctggg ccggcagctc
    16441 aataacaaaa tcaagtattt tcattggcgc aattattaaa agaagacggt tcgatgcggg
    16501 gcagaaaatg aataatcctg acctttttca aagtaaatcg attaagtttc agaaaaaaat
    16561 attttgttaa actttagaga tggtttttta ttgttttccg ctctttttct gcagaaatta
    16621 ttttaataaa tgacggctcg cccagccttt aagcttttgg aaaaggagct tctctgaatc
    16681 catggattat gtcctgtctc ccaaccattt gcgcagccag tgttcaggca tccaagaata
    16741 ccagccgtgc cgtaatcgaa aacgtgccag ggggcgatag acggctgcca gcattctcag
    16801 ggctgcggaa tcggattgat agctcatttt ccgatcggcc agaatgctta cgatctcaat
    16861 tttttccatc aatgctttcg tgtccgcatc ctggtcgcgt cccatgtatt gtgtccagtt
    16921 aaagtttccc catccttcca gtgttgaagg tgttacatca tctttgtgaa tgacacgttc
    16981 tcgcaggctg gtgccgggat agggtttata gaaatggaag gcgctgatca aggcataagg
    17041 attatcccgc aataatgtcc aggccaacgc cgcggtcttc aggacatcct gagcggtttc
    17101 cgtcgggaag ccgcacatga aattgtaata caccttcatg tgaggatggc gcgacaggcg
    17161 tctattgaag gccagtacct gttcgggatg aattttttta ttgagaatgt ccagtatgcg
    17221 aggtgatccc gattcgacgc caacctgtag aaaagtgcca ccggcttttg taatcagttc
    17281 cagatcctca tcctgaaggt catcaaccgt gtcaatgcgg gcaccctgaa accccatttt
    17341 aattttccat tttcgattca gaatgcctct catgatttcc cgcgtacgtt catgatcgat
    17401 aaaaaatccg tcgtctacaa agtgaaaagc cttcacgccg tgcgtttcaa caaggggttg
    17461 cataaactcc aggattcgcg aagcgctcaa ggcccgccag cggcgccggg agtataaggg
    17521 attatagcaa aacgtgcagt taaaagggca gcctctcgat gtctcaaatt ccaacacgcg
    17581 atcttcatca aaatagcggt gcagatactt gttaacatca atcaggtggt aaggtacggg
    17641 aggcaggtca ttcaaatcca ggaagggacg gtccggatta ttcacaatat cgccgcggtc
    17701 tttatacaca accccttcca gaccgtgcaa tgattgtttc tgttccagac gtaaagccag
    17761 tagcaggaac gtttcttcgc cttctccttt gaccacaata tccaccaggg aatgttcaac
    17821 agtctgcaaa ggaaacagcg tcgcgtgaac gcctccccat atcaccggca ccgctgaatg
    17881 ctgcttgacg aaggccgaca tctccagagc atgggtgatc tgagaaccgg tcatggacgt
    17941 tacgccgaca cagatgggat tttgcgacag agctttttga aggtctgatt tccagtctac
    18001 agaaatccgc tgatcaatca ggatgattgg gtagccggct tcatccagtt tagaggccgc
    18061 cgccagcagc gataaaggca cccacgggcg aaatactttc gcgtaaagcc cttctgcagg
    18121 ccgcagcaaa acaaccggtc tcattttctt cttgtttgcg ttgacggtaa taagaatatc
    18181 cttttatttc aatttcagcg gacagtattt agttggcttt atgttgtgcc gcatgagcaa
    18241 aaattttaga gaagctgtca ccgtatgtca atcaatattc aacgcaatcc aatctaattt
    18301 tctccggtgt attccggctg gttaatatca aacgccatat aacgcggatg cgcataagat
    18361 tcgcaaaatg ttgattgaga ttatgaaaac gttaaggact gacgggcggt tacgacgacg
    18421 cgtgaaactt tcggccgtat ccgtcttgtt taacgggact caaagggata catgccggtt
    18481 gtctgaacaa ggattagtct gctgttttct ttcagtatga caaagccggt ctgtacagct
    18541 gaatccacat gcagaatatc caaaatattc gatcccatgc ccattgattc aagatcatga
    18601 agatgcgcta tattgctcga tacgatgtcc gttttaatgt tgtccggaga gtaatggttg
    18661 aagatgtcgc ttttaaattc tatgccgtat tttgagagat aagccatgtc tctgacaaag
    18721 ttttcatttt ttgcgttatg gatcgactct ttcaggcgac gggcaatgtc atacaccgac
    18781 agattctcga cttcatcttt cgtgaattcg gtggtgcaat ccaagtatgc attcccgata
    18841 tataacggat caatatccgg atgcacgtct ctgagattaa ccggattccg cataacaatc
    18901 ttgcgggtat caggcaggag atgctgatga tattttttca ttagcacggc attgataatc
    18961 tgattgttgg agatgcagtc atccccgttt ccccgttttg cgtgcttttt aatatcgttg
    19021 agaaattcac aggtgaaata gtccgtatga cgatatgttt ttatattgtt ttcataatga
    19081 atccggtttt gaattacatt gcttaactta gataacgacg gcttgaacat cttgtcaata
    19141 tggccggaat tgagggggct tcccgtaaac agccgttgtt ttgagggtcg agagaatgga
    19201 ttgccgtcga tgattgagac ccatgcaaac aagaagagaa agaaagagat cccatcggcc
    19261 acggcgtggg aacagcttac ggcgccaacg gtgccgtcct gggttgagaa ccccgtgacg
    19321 gcgaaaagag gttcgccggg caatgtctta acatgtacca tcgattgcct aatgtcatca
    19381 ataggaagct ttgcgacggg tgcttcaacc ggcggtagaa tattcacggt gaaaccgtcg
    19441 gaggcgaact gcaatgcata ttggtgatgg ccaattttaa tcaggcgact ggaaaaaaga
    19501 ttgtaatatt caatcgtctg aagcagacat tctttaagtt ccgagatggc cttctttttt
    19561 ttgtaaaaaa cgattatttc aatcggtttt gaaccaatga gttcttcatt ttcaatgatt
    19621 gaaagcggca aaatattttt gccggcgttg ttttcaagat atgcacggat ctctctattc
    19681 ttcatcaacg ttcttgcgtc atgggattgc tcgcttataa ttcaaaatgg aatcaatttc
    19741 aaatagaatg actaggtcat cggttttctt tttgcagaaa atgcgcgaag catgataaga
    19801 ttatccggaa tcatacagcc catggacatg acggatcaaa acgacatggt tgattatgtt
    19861 atagagcctc tgacgttcaa atatcttctg ccggcgctca gactggttga ttctgttttc
    19921 cccagaagag agcagggcgg cgaacgggct gatctagcct ttacggccag tctcctgacg
    19981 ttaggaagac gcattgccaa atggatggga tatcccatgg tccggtattg gattgtgcgt
    20041 gatgttgcga cgaatcggat ttacggcacg ataggacatt atgtgcgtgc ggatgatccc
    20101 gatgcgtact ggggaggctg gatgtgcgtt gatccccggg cgcggggatt gggcatcggg
    20161 aaaaaactca tcaattttgt tgtgggcgag tcggttcgca gaggagacag gccgtatcac
    20221 cgcttttaca cctcaacaga tcccaatgag gcgaaagccg tggcgatgta taatcgtgcc
    20281 tgttttaacg tttataagca agaatgcgat cccgtgacag ggtatacaag gttatattat
    20341 caggtgcgcc ttgacgacgt gactctgccg gtattgtaat ttatgctagg cgttgttgaa
    20401 gagaattgga gaaaaattaa tctgtgcgaa aaaaagtcgt tttattttcc ggagagagtg
    20461 ttttcggcag cacaatcatc aacaggcttc tgaataagca atcctttgat ttaatcggtg
    20521 tattttattc gacaagagta tataaaaaat cctccagtat attctcgttt tatcaagtgc
    20581 tttccataac gggaatgcaa tattactgga atattgtgaa gctgaatgct ccatttgcaa
    20641 aattgtttta tcctgaggtt acccgaaccg ggaaagtctg ccggcaaaac ggtattccaa
    20701 tcttttatgc ctccgatgtt cattggcccg caaatattga aattatcacg caacttaagc
    20761 cggatattat tttgtccgcg tactttaatc agctgataaa agaacccatt ctttcttttc
    20821 cggctgaagt gttgaatatt catcccggca ttttaccgga gtatcggggg cttgatccgt
    20881 tattgcagca gatcatacat ggtgaagatc atctgggcat gaccttgcat agagtggatg
    20941 aaacgtttga tacgggagaa atattgagac agaaaacaat gctgcgggat aaaacaaaat
    21001 cgctttttga actttacatg gaaatttggg aacaaggtgt cgatatgctg gaagactata
    21061 tccgtttcgg caagcaacct attgagaatc tgactgaaag acagcagcgc agctcatatt
    21121 tttcctggcc gaccaaaaaa gatgtgcaaa tttttttaga aaaaaaccgg tttttttaaa
    21181 tcttcattgg caaatagaag acattattgc cgtcatgaca tcaggcttcc tggataggat
    21241 ggagtggctt tctctaaaga ttgtctgatt attagctttt ttagtgttgt aagaccgcag
    21301 gattttctgc gtatttgatt ttgaggattt ctttcgacga atggtgtcta ccgggatgtc
    21361 ctgatgcttg tcgaatgatc tttttaagaa gctctcatcc tctgtggata ttacatccta
    21421 tcgtatacaa gattacgaac aaaattaatc gtcaactgtt cttatcagat ttgataaatt
    21481 cttgttctcg ccacgaaaaa ttgccggtat agccaacaac ctttctcctg gtgtactcaa
    21541 ttccgctcca tttcatactg cggtaataag gcatggaaag aaagttgatt gtattaagaa
    21601 tctggaccag catcggcaga aatactgccc ataaattcaa tttgatattc agcgcttccc
    21661 gttgagcttt cgttaataaa gctgtcatga ttatgaagct gtacgtcatg tcaatcaagg
    21721 gggataatag ggcaagtgaa ctcaaaggat accaccaggc aacaaccatt gcacaaaaaa
    21781 acatgggcat tctgattttg gtcattaagg aagagagcag ccatcgactc ctgtttgtcc
    21841 agaaaaattg caagacttgc cggttggtaa agctgataac atcgcgccat ttttcttttc
    21901 gatcaggcgg gtatcggggt acgatgcaat taggaacgaa aacagtttca tatccctgtg
    21961 tgcgtagagc gttattaata ttgttgtctt ctgaggttat aggatgtttg ccttggccgt
    22021 ttatgctcga tattaagttt gctttttcga acactgcttt tgatatggcc agccccccgc
    22081 cccagatata cccatgtttt cctgccagaa aaggggcgga gttgatgatc cataaggcct
    22141 gaacccatga cgctaatcct tttccgggaa gataaagtct cgccgaagtg acggcgccga
    22201 gcttggaatg accatccgta aaaggagccg ccagattcct taaccaatcc atgaagggac
    22261 gggcatcggc atcgacaata acatatactt cagtttttgg agaggaagaa tttattgccg
    22321 ctacgacatt gctgttcttt tgtgttctat gaatgcattg gttcttggag agctgaacaa
    22381 catcgtcagc aatgactatc ctgacatttt catgcttgtt ggaatagttc ttcagcaacg
    22441 gcagagaagc atcgtcttct gtcgttacaa aaataacctc ccaggtcccg ggatactgct
    22501 gtgctaaaag agcctcaata ttttccggca cgattttccc ccgttggggt gcgataaccg
    22561 tcagcgccgg atatttcccg ggtttatgac gtgaccagtt tttcacatgc gatcgcaaag
    22621 tcagagccat tgtaaattct atgaatgaac tgacgattgc cagaaatgcg caagtatatc
    22681 cgatatattc aaacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcag
    22801 gaagtacggt ttcatcgata caactgcctc ccttaaaagc ataactgcat tcgtaacaaa
    22861 agagattctt cggatcattg cggcggaatc ggtctcgaaa agcacgataa gctgagcctt
    22921 cccatatgtc ttcaaaagag ttgttatgca aattccccag aacaaatctg tcgtcataat
    22981 cataagtgca ggggcacacc gttccattcc agtgaatcgt ggatgaattg taaagatttc
    23041 ggcacgcatt gcgtttgcgc cgaacaggcc ttccattttc atcgaagata aaacggctgt
    23101 aacgcggatt ctgcggcaca agatcgtaat tttttattcc atccggcttg tccccgtagg
    23161 tattgtccgc cagcggattt aatgtcttca aggtaagcac gtttgcgccc agtttacgga
    23221 caaaagaagg taatcgttct atttcgtgtt cattatgttt catgacgata aaacgaaagt
    23281 tgatcagcgg tgtcgaagat cgtaacacct tttttcgttc aacaagcatg cgtaggccgt
    23341 ctacggattt ctggaggtct cctttatgac gatatttttc ataggtcgtc tgactgattc
    23401 catctacggc aaagataagc gtatccaatc cgcaacggat ggcctcatcg gctgcgtgtt
    23461 gatcggcaaa caaatggcca ttggtgctgg atatgatccg aatctttttt tcatgggcat
    23521 atgcgatcat gtcaaaagct gacggatgta taaaaggttc tccccagtcc caaaagaata
    23581 ggaaaaacaa atattctcct atctcatcca aaagatcacg gtagacagat aaatccataa
    23641 aaccgcgcgt acgattcatc tggctactta caggacagag ctcgcatcgt aaattacaat
    23701 tattggtagg ctctatctga agatgggtgg ggaatcctaa tggaacgaca ggccgcaggg
    23761 gagcaaaggc ctcgacgcgc aaccagtttg tcagcttttt ttttgaaata tcattaaatg
    23821 tataaggaag ggaatcgttc tgaatcgtga gacgtcgtcg aaataaattg taagcaactc
    23881 tcattgcttc ctctattttc cttatcaata ggatttagaa caaaattttt aaggctgtta
    23941 ttattgtaca tcaataaaaa atactgtcaa tcaccctaaa aggattaaaa tattaattga
    24001 cattgatcaa aacttatctt cttcggaggg cagtataaaa aacgtgctct ttgtgtcaag
    24061 aaaatcttgg ccatacccac ttgccgggcg caggattttg gaatgctgaa gatgacatat
    24121 ccatgcgtat acattccgcc aggggaaagg at
//
LOCUS       DAONQZ010000033        23420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2433_length_23420_cov_17.671688, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000033 DAONQZ010000000
VERSION     DAONQZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23420
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2433_length_23420_cov_17.671688"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLR13_06880"
     CDS             <1..762
                     /locus_tag="PLR13_06880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQC19098.1"
                     /translation="MVEILDTIIPLEDQECADLLKQELTRMGVVIHTSTKLESLKDTG
                     SSVAMVAQKDGKHLEISADYALLCTGRKPLLFTAELDKCGIEYTQAGIKVDKKMMTSV
                     AGIYAVGDVTGGMMLAHRAMQQAKFAVERIFGGDDFAYNEDCIPSVVYSHPQIARVGY
                     TQKRAGEEGLSVEVVKSPYSANIIARAELTGQGFVKAVFHQNKLIGATIVGEDAAELI
                     VPLALIVSNQMTKKQLKGWVLPHPTLSEVFALLTG"
     gene            777..1127
                     /locus_tag="PLR13_06885"
     CDS             777..1127
                     /locus_tag="PLR13_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQC19099.1"
                     /translation="MKSINYKTVAPTHFDKGSAKGVAGRVVVGKADGASNFCMRVFEI
                     APGGFTPKHSHPWEHEIFIHAGSGEVYGNGQWNPIDPESVIFMPAGEEHQIRNTGKET
                     IVFVCLVPSVAPEL"
     gene            complement(1140..2477)
                     /locus_tag="PLR13_06890"
     CDS             complement(1140..2477)
                     /locus_tag="PLR13_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQC19100.1"
                     /translation="MQKEKNENDIIVIGAGAAGMMAAGRAGELGAKVLLLEKTDDCGK
                     KILVSGKTRCNLTNAAELNHFIAMYGDNGRFLFSAFHHFYRTELLDFFNHHGLATKVE
                     RGGRIFPVSDDAHDVVRIFKKYLSENNVTLKVNTKVTGITMKDDSVYGVKTKDQDYRC
                     KAVVLATGGATWPSTGSTGDGYKIVAARGHNIVKPKPALVPLIVREQELAQSMQGVSL
                     RNVRATAFQGEADSIDSRMTPDCIYGRGEKKSPRHPVIESRFGEMLFTHFGLGGPIIL
                     LMSLSVVEALEKGLVSVLIDLKPALTREQLHKRLQRDLDNFSKRKISGILKEYLPAKM
                     IEPFIGLTGINNEKLAHQINAAERGKIVEILKALRFNIKASLPLEKAIVTAGGVSLNE
                     IDPRTMASKLISGLFFCGEVMDIDADTGGYNLQAAFSTGYLAGEKAVEYIKAS"
     gene            2852..3139
                     /locus_tag="PLR13_06895"
     CDS             2852..3139
                     /locus_tag="PLR13_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19101.1"
                     /translation="MKKVVLLVVALIFSFSMTVAAVEKAEVPDTSEPVKVADIEKDDI
                     KKGDVKKDKTKKSKKKNVKKSKDKKSKKSSKKDEKIVEDAESEVPAAPAAK"
     gene            3492..5042
                     /locus_tag="PLR13_06900"
     CDS             3492..5042
                     /locus_tag="PLR13_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19102.1"
                     /translation="MVKTILKKITAFNNRLTVRSKLLVLIVSGLALTMGVWGIIQLAA
                     LERILVEQQAKRLKDVAETVSTFYQDFPTKKGLKALESALHDHLQADGRLARIDIFDI
                     GQKHFDYVAGASRDPYQWADNIVMSVMKKNKLHYMKIMTENGPALGLLYPVAGNTKSV
                     RIVIGIISFSRANEEIVHRAGRLLIISSAGLLIFILVVLGLSYRWIIGKPLKLIIHAI
                     DEFQEGQYDNRIPIVHRDEWGQISRHFNMMADEIQDVMERNQELTKHLEKRVHEETIK
                     VFQLQKQVTDLKQLTTLGQLTANLAHDLGTPLHSIAGLAKLLLEKGGLPENVEHKLNL
                     IVQQTLRLDAVIQNVRKAIRLPDPSFEALPVEQIFHEMLSLMEPFLQKNNVESVVTIT
                     NPVSPLYVDRYRIQTALMNVMQNALEAMPEGGTITMSAHEVPCENFVAITVSDTGYGM
                     TPDVVENVCEPFYSTKTSKGLRGLGMAIVRDVMKIHRGQMEIKSSRGQGTMVILCFPK
                     LKDADHFH"
     gene            complement(5091..6413)
                     /locus_tag="PLR13_06905"
     CDS             complement(5091..6413)
                     /locus_tag="PLR13_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC19103.1"
                     /translation="MKKRILVVDDDAVACEFLEETLKRDGYDVTLCSSAWEALRQNIA
                     SYNLVMSDIRMSGMDGLQLLEELQKQSPDLPVILMTAYGSLETTMAAVNKGAWDYISK
                     PFSPEDCRTLVRKVLELRKLRHKGMKLQPAKVEESKFIGSSAMMVDFYKQIARIADSS
                     ASVLIEGESGTGKELTARSLHNMSSRREKPFIIVHCGAIPDNLLESELFGYNKGAYTG
                     ASHQHTGLLETAEGGTVFLDEITEMSTALQGKFLRFMQNGELRKIGDNAVRNVEVRVV
                     AAANKKIDDEVKKENFRLDLLYRFIVRLRIPPLREHKEDIPLLTEALLKKLGDTNVSV
                     SPDAMEMFMAYDWPGNVRELENVLQQIMLLSPFDEILPENLPERFHSSTDRKDILNGP
                     GLSPLEEAEKEKILQTLKETSWNQSQAAKRLDIDRKTLRTKIRRYSLS"
     gene            complement(6422..6859)
                     /locus_tag="PLR13_06910"
     CDS             complement(6422..6859)
                     /locus_tag="PLR13_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQC19104.1"
                     /translation="MPEYISLPITRTHLVRYAGASGDFNPLHHDQTFANTIGLQNVIA
                     HGMFIMAIAGEAITSWIGNKNIKKFHSRFLAKTEPVDWDNFENTKDRATIVVKGKVIR
                     LFVENGEKLFECKIVAENLLGSKKLSGSFIAVVTGEPPRDVNE"
     gene            complement(6903..7367)
                     /locus_tag="PLR13_06915"
     CDS             complement(6903..7367)
                     /locus_tag="PLR13_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HQC19105.1"
                     /translation="MADQSKIGMEFPASTFIIEKDKIIEFAIAVSQKEMKNQIHPVYH
                     DEKAAKAAGYEGIPIPPTFLTSSFFWTGDGLTGMVTALGINLKRLLHREEEYEYFCPI
                     HAGDIITRKMKVADIIKKGKGARTLDMTILEVEYINQRNELVAKSRTTLTEI"
     gene            complement(7566..8762)
                     /locus_tag="PLR13_06920"
     CDS             complement(7566..8762)
                     /locus_tag="PLR13_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19106.1"
                     /translation="MINLEMTQKMMDVKSGMNNMASSMLRPVSRHYDEHEHEYPKELD
                     VFRSIKIMSRPKKKKEGGETKPDKPGAKKLGANLSMVIVIEEMCWGDLGLLLSLPNAG
                     LGNAAIAAVATDEQMERFGEKWASMAITEPGSGSDSGSITARATLEGDEWVLNGEKIF
                     VTCAERAEVAVIWATVDRSAGKAGIKSFVVEKGRPGFKLEKLEHKLGVRASDTGTYVL
                     DDCRIPKDNILGSAEVKQSTEGFKGAMKTFDNTRPLVASMALGVTRASLDFTQEKMEA
                     SGITLDYEKNPNNLSAMEKEFYLMEAHLDAMRLLTWRAAWMMDNGEFNNLEASMSKAK
                     AGRYGTFICQKCCELLGPLGYSCENLAEKWMRDVKITDIFEGTGQIQHLIVARNVLGY
                     SSKQLK"
     gene            complement(8774..9883)
                     /locus_tag="PLR13_06925"
     CDS             complement(8774..9883)
                     /locus_tag="PLR13_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19107.1"
                     /translation="MEDRDFGFGMSEEQSSMKDTVAKLVKSMVSGNPAHDMDEARKLP
                     REIIDKVWELGATVSTVPEEYGGYGMEYSPIMNAIFVEELAAGDMALAIASTLPATFI
                     YTILQMGTDEQKKKYLPLYCTPTYQACTVAINEPLFRFDAVSLETKAVKNGSKYIING
                     KKCFVPMAKDSGHMLVAADIDGKMDLFIVEGNNPGLKIGEREKNLGMYALESFPVVLE
                     NCEVPDDDRVGGDNGCDYSRFLQKTRIAMSAIGTGVSRASYEYAREYAKERIQFGEPI
                     ASRQAIAFMIAEMAYEVDAMRLMTWKAASALEAGRDAKKEAYLAKLYAGEMTMKLTDF
                     GVQILGGHGYIRENPVERYYRNGRGIAILEGMATV"
     gene            complement(10076..10645)
                     /locus_tag="PLR13_06930"
     CDS             complement(10076..10645)
                     /locus_tag="PLR13_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQC19108.1"
                     /translation="MAKFKAPSGVSVNDFFEKLVPEQFKEVIAGANLSSLTGKEVNLQ
                     FNVNDQQYCLKIKDGTNLDVVKGSVDKPLLTLSLSEQVFLDAVTGKAEGLIDRFTDPV
                     EIADPMRLKNLMDTKGTLNLKLKQDGNEIPVTMIFNGEEAPKVDISLDLQDWIAMQNK
                     QTTGQNLFMNGKLKFAGDMTLLMKLQTLI"
     gene            10947..11603
                     /locus_tag="PLR13_06935"
     CDS             10947..11603
                     /locus_tag="PLR13_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC19109.1"
                     /translation="MQKLSKPSGSRSSSNEGNVRERILKAARDVFTVYPFKEAGTRMI
                     AKQAGVEHPMIHYYFGSKENLFYAMAEDLYHETLSVQEAWLDGMENMSLKEGFELFLD
                     RMLDYTMQKPDALRIAALNVVNIGNLEEIPGYRFIIMSMASMRRIIEEKLSLKGSNRE
                     IEMFIYCFYSLMISLIGARSCQAQILNMNPLEEKYRTWVKDASMTLFYPWFKKILVGR
                     "
     gene            11762..11899
                     /gene="rpmH"
                     /locus_tag="PLR13_06940"
     CDS             11762..11899
                     /gene="rpmH"
                     /locus_tag="PLR13_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQC19110.1"
                     /translation="MKKTLTNKTNTKRKKTHGFLVRMASKAGREVLNRRRAKGRKRIA
                     V"
     gene            12298..12525
                     /gene="yidD"
                     /locus_tag="PLR13_06945"
     CDS             12298..12525
                     /gene="yidD"
                     /locus_tag="PLR13_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQC19111.1"
                     /translation="MLRKIFINFFVSFIHLYQACLSPFFFAPCCRFYPSCSQYAIEAI
                     KLYGPAKGSWMGIKRILRCHPLNPGGYDPVK"
     gene            12544..14259
                     /gene="yidC"
                     /locus_tag="PLR13_06950"
     CDS             12544..14259
                     /gene="yidC"
                     /locus_tag="PLR13_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQC19112.1"
                     /translation="MDKRTILAVVLSLAVLFAYQIFFAKPPVPPETVPVRESHQTAQK
                     PTPIKENSVVKQQAVSPDASTTKQKVKNVLPAQEESSRDIKVETANYTAVFSTKGAAL
                     KSFQLKGYQKECTKCVGDIWPAIKNFVTGKKQPAGEKNNDLIEVVSLQPDMPYPLTLT
                     FPESSGEIAADSVYKTDTAELNMVKNSKKQRLVFSRVFNDVRVEKIYIFNPDNFSITL
                     EVNISNLTDVPITKIPYLNWYDYVDPKEKVDGYSKEGPVAYVSKSIERWKSADVSKEQ
                     TIGPDVSWGGVERKYFMAVIIPQDPSLTSFNMSRDDHVIAVNLKGAKNIIPPEQSGSF
                     KYNLFIGPKDYAMLKKLGLSLEEAIEFDSVIPGLKWLSIGLLLFIKFIHQFVGNYGVA
                     IIILTILIKVLFWPLGNISYKSMKEMQKIQPQITALREKYKNDQAKLGQETMALYKTH
                     KVNPFGGCLPILIQIPVFIGLYNTLLYAIELRHSPFFFWIQDLSAKDPYYITPIVMGA
                     TQFIQQKMTPTMGDPMQAKIMLAMPIIFTFIFLNFPSGLVIYWLVNNIISIGQQIYIN
                     KKLTD"
     gene            14293..15177
                     /gene="jag"
                     /locus_tag="PLR13_06955"
     CDS             14293..15177
                     /gene="jag"
                     /locus_tag="PLR13_06955"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HQC19113.1"
                     /translation="MSSEFIEIEEKTIDEAIEKACQTFGVPREKLNIEIISGESGGFL
                     GLFSKKAKIRASLLALDIDFSFSDKPKEKTDKPREKNEPRSKADHYREKSEAKVLPKR
                     KPEKEVKEISEEKSPVKKEREAFIPNSPLSLKAQELLEGILQRISPEYKANVQETQEK
                     IILGIEGDESGLLIGKRGQNLDAFQYIINKAINKIDSEHKMILIDSGEYRKRREDFLL
                     DLAEKIKKKVKKTKKPVSIANMNAHDRRIIHLVMQEDESLVTQSRGEGKYRKIIILPA
                     KNGSSRRTKEKKKDINQS"
     gene            15196..16575
                     /gene="mnmE"
                     /locus_tag="PLR13_06960"
     CDS             15196..16575
                     /gene="mnmE"
                     /locus_tag="PLR13_06960"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQC19114.1"
                     /translation="MLRSDTIAAAATPFGMSGIAVIRVSGPEALAVARRIFRPSNRPC
                     DWKSHHLYHGDIVCADGETVLDEILIAFMRKPRSFTGEDVVEISCHGNPVIIQSILDQ
                     LQKLGCRLAGPGEFSQRAFLNNRFDLSQAEALASMIGAQSEKAYAVGLAQLKGSLSKE
                     IETIRGLVIEALALMEASIDFAEDTAEAELVQIPPQINQAMERMQRLLTSYRFARAYS
                     EGVHVVITGKPNVGKSSLLNALAGKKKAIVTDIPGTTRDLIADTIHIGGIPVHLTDTA
                     GIREPQNVIEQEGIALVWKKLAEADIVIIMLDVSKPLTEEDKITIDKNTSEKIIVAVN
                     KIDLPRSWETKSIANLFPPHTKFLEISAKSGSGLEELKRTILDLSVDGETESTSGAIL
                     TNLRHKHAVEQSLKNIQNAKESVTAGLSAEFIAFDLREALDHLDEITGKKINDEILDR
                     IFSSFCIGK"
     gene            16737..18398
                     /locus_tag="PLR13_06965"
     CDS             16737..18398
                     /locus_tag="PLR13_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQC19115.1"
                     /translation="MLTDIGISVLAATALGFVFQLVRQPVILGYLVAGALIGPAMGFN
                     LVSEAASIEVISEIGLILLLFIIGLELNPSKLLASGRQLIYAGIGQFIFCVLIGLGFF
                     LLLGYELSRNNIDALYLALFCALSSTAIVVKLLYDKFELDTFPGRITLGILIFQDLWA
                     ILILALQPHFTNPQFLLVFIALGKSVALLAIGFLLSKYLLSWIFKKISQTPEMVVAMS
                     MAWCALMAGIGSWIGLSMEMGALIAGVAISTFPYSMHVTAKVLPLRDFFLTLFFLSIG
                     MKIPFPDAAILGMALIIVLFVIVSRFLTIYPLLTLAGSGRRTSFIASLNLAQISEFSL
                     VVAALGVHYGHIDQRMMSLIIYAMAMTSVLSSYFIKGNHAAYLVFNRFLEKRGFPART
                     KENHDEEEKNVYPIVILGFHRGAREFIGRLETETPDLLKKLLVIDFNLENLKELSNKN
                     IKGIFGDIGSMDTLEHAHITAAQIIFSTIPDMLLRGTSNKALVTSCRALAPSAYIVAT
                     ADSADQIDVLRKSGADEVLLPYVLIGEKLTRFVEQTCLTAESCKL"
     gene            18434..19354
                     /locus_tag="PLR13_06970"
     CDS             18434..19354
                     /locus_tag="PLR13_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQC19116.1"
                     /translation="MGKKVGLVLGGGGVRGFSHIGVLKVLEEEGIDIDLIVGTSAGAL
                     IGGAYASGQAPDEIESKIDAYLQSPEFDDSRMKSIGLSFTQEEKNILKKAQQFIMNRV
                     LFVQAFFKPSILPSHDFLSLMNYFLPDLDIKETRIPLHVVSTDLISGKKIVFSEGSLR
                     KVVLASCAVPGAVEPVRYGDWLLADGGITSLVPVHAAREAGADVVIAVTVDRDLQTNL
                     RIETAKDVVFRAGEITPSVLEAAELADADVIIRPQVGNLHWMDFKRAVDLVKAGESAA
                     RDSLKSIHASLPIYRRITRLAGKLFKQSRL"
     gene            19604..20080
                     /gene="rpsF"
                     /locus_tag="PLR13_06975"
     CDS             19604..20080
                     /gene="rpsF"
                     /locus_tag="PLR13_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQC19117.1"
                     /translation="MQRYEVVAIVLADLIEDEINALIERSQTIITDRKGVIAKVDKWG
                     KRHLAYEIKKQRDGYYFLIDFAGNGSIVNEIERNYKIDDRVIKFMTVKKEGATTAEGI
                     QQEAAAAEAKRVQAKVEAEAAAAEGKQINQIEKAPRESRPRVRKDHNKEETQTKGE"
     gene            20090..20359
                     /gene="rpsR"
                     /locus_tag="PLR13_06980"
     CDS             20090..20359
                     /gene="rpsR"
                     /locus_tag="PLR13_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQC19118.1"
                     /translation="METQNGEKQGRYPQKKFFHRKKFCRFCSDSNIKIDYKNTAILNN
                     FVTERGKIMPRRITGNCASHQRELALAIKRARQIAIMPYVTADGR"
     gene            20530..21507
                     /locus_tag="PLR13_06985"
     CDS             20530..21507
                     /locus_tag="PLR13_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HQC19119.1"
                     /translation="MSSFSFFKKSGAPASGNSFSGLLSAVGLFAFAAVFVPLVGPVFL
                     FLLPMIAFVNGATNGIFKTALCLLFSLAVLMLLSVGLNLDVPAVAIFTMGMAGLLMAH
                     MAAENYSIEKIVTGPVLLIFGAIGFYFVYDAVGLGVNPWLLVKNFITVTISETIKFYS
                     QMPLKPDDISMIKDNENNIINSLIHIFPSLMVILSVFIIWANLLLGKTYLRRFGIVYA
                     QMAILARWKVTDWAIWIFIISGLLLFIPQKNFNFIGLNVFLIVCLVYLLQGLAIVSFL
                     FQNKNVPFLIRYIFYFLIAVQQILMIPIAVIGLFDVWFDFRKRFQKNHL"
     gene            21552..22004
                     /gene="rplI"
                     /locus_tag="PLR13_06990"
     CDS             21552..22004
                     /gene="rplI"
                     /locus_tag="PLR13_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQC19120.1"
                     /translation="MKVILKQSVRSLGKAGTVVKVNDGYARNFLIPKGLAIEADEKNI
                     RLFEHEKNNILKKAAKEHKNAQDLADVLSRETITIARKVGDQDKLFGSVSAKDIEAAL
                     KEKGYDINRKMIMHEHGEHIKELGEFAVKIKLAHDVEAQITLKVVGES"
     gene            22004..23368
                     /gene="dnaB"
                     /locus_tag="PLR13_06995"
     CDS             22004..23368
                     /gene="dnaB"
                     /locus_tag="PLR13_06995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQC19121.1"
                     /translation="MKEKDPSQYKLPPQNLEAEQSVLGSILLENSATNRVFEILSERD
                     FYSEAHRKIFIVIKDLSEKNEPVDLITLSNALKDKSLLDSVGGTTYLASLVDNVPSAA
                     NAANYAKIVKEKAILRGLITSATEIIDNCYETGSDVDTILDKAEHSIFEISENKVRQD
                     YFPIRDIIKDSFRSIEDLYSRQQLITGVPTGFGKIDDLTAGLQKSELIIIAGRPSMGK
                     TAFALNIALHAAMEAQIPTAVFSLEMSKEQLAFRLLSSEARVDAQRLRKGFLGETDWP
                     KLTTAAGRLSDAPLYIDDTPAITVLEMKAKSRRLKADKGLGLVVVDYIQLMRSSYSHD
                     SREREISDISRSLKALAKELNVPVVALSQLNRQVESRTNRRPQMADLRESGAIEQDAD
                     VIAFIYRDEVYNKSEDNIDKGIAEIIIAKQRNGPTDTVKLAFVDKYTSFENLSRVELP
                     EGQH"
BASE COUNT         6184 a         5378 c         5582 g         6276 t
ORIGIN      
        1 atggtggaaa tcctcgatac gatcattcca ctggaggatc aggaatgcgc cgatctgctc
       61 aaacaggaat taaccagaat gggtgttgtt attcatacat cgacaaaact ggagagcttg
      121 aaggatacgg gaagttctgt ggcaatggtc gcgcaaaaag acggcaagca cctggagatc
      181 agcgccgact atgcgctcct ttgcacaggc agaaaaccgt tattatttac ggcagaattg
      241 gacaaatgcg gaattgaata tacacaagcc ggaatcaagg tggataaaaa gatgatgacg
      301 agtgttgccg gcatttacgc agtgggcgat gttaccggcg ggatgatgct ggcgcaccgg
      361 gcgatgcagc aggcaaaatt cgctgtggaa cgtattttcg gaggagatga ctttgcctat
      421 aacgaagatt gcatcccttc ggtcgtttac agccatccgc agattgcgcg ggtgggttat
      481 acccaaaaac gcgctgggga agaaggcctg tccgttgaag tcgtcaaatc tccatacagc
      541 gccaatatta ttgcccgcgc agaattgacg gggcagggat ttgtcaaggc agtttttcat
      601 cagaataaac tgattggcgc gaccattgtc ggtgaagacg cggcggaact gattgttccg
      661 cttgcactga tagtatccaa tcaaatgacg aaaaagcagt tgaaaggttg ggtgctgccc
      721 catccgacat tgagcgaagt gtttgcatta ttaaccggtt gacaagggag tcccttatga
      781 agtcgatcaa ttataaaacg gtcgcaccaa cccattttga caagggttcg gcgaagggcg
      841 ttgccggacg agtcgttgtg ggcaaggctg acggcgcgag caatttttgt atgcgggttt
      901 tcgaaatcgc tccgggagga tttacgccga aacattctca tccatgggaa catgagattt
      961 ttattcatgc cggttccggc gaggtttacg gcaacggcca gtggaatccg atcgatccgg
     1021 agagtgtcat tttcatgccg gccggtgaag aacatcagat cagaaacacc gggaaagaaa
     1081 ccattgtgtt tgtttgtctg gttccgtccg ttgcgcctga attgtaatgc cggagatatt
     1141 taagatgcct ttatgtattc gacggctttt tcaccggcca ggtaaccggt agagaaagcg
     1201 gcctgcaaat tgtacccgcc ggtatcggcg tcaatatcca tcacctcgcc gcaaaagaaa
     1261 agtccgctga tcaatttgga agccatcgtg cgcggatcta tttcgtttaa cgaaacaccg
     1321 ccagccgtaa cgattgcctt ttccagcggc agagacgcct taatgttgaa acgcagcgct
     1381 ttcaaaatct caacaatctt tcctctttcc gcggcgttta tctgatgcgc cagtttttca
     1441 ttgttgattc cggtaagccc aataaatggt tcaatcatct tggcgggcag atattctttc
     1501 aggatgccgg aaatttttct tttgctgaaa ttgtccagat cccgttgcag ccgtttgtga
     1561 agttgttcgc gggtcagcgc cggttttaaa tcgatcaata cggaaaccag acctttctcc
     1621 agggcttcaa cgacggacag gctcattaat aaaatgatcg ggccgcccag tccgaagtgg
     1681 gtgaagagca tttccccgaa acggctttca ataacgggat gccggggcga ttttttttct
     1741 ccccgtccgt aaatgcaatc gggcgtcatt cgggaatcga tggaatccgc ttcgccctga
     1801 aaagccgtgg cgcgtacgtt gcgcaggctc actccctgca tggattgagc aagctcctgt
     1861 tcccgtacaa tcagcggcac cagcgccggt ttcggtttga cgatgttgtg tccccgcgca
     1921 gcgactattt tgtaaccgtc gccggtggaa ccggtggaag gccaggtggc cccgccggtg
     1981 gccagaacga cagctttgca gcgataatct tggtcctttg tcttcacgcc gtaaacggaa
     2041 tcgtccttca tggtgatgcc ggttacttta gtgttcactt ttagagttac gttattttcg
     2101 gaaaggtatt ttttgaagat acgcacgaca tcatgagcat catcggacac aggaaatatc
     2161 cgtccaccgc gttccacctt ggtcgccaga ccgtgatgat tgaaaaaatc aagcagttcc
     2221 gtacggtaaa aatgatgaaa ggcgctgaaa agaaaacggc cgttatcgcc atacatggca
     2281 ataaaatggt tcagttcggc tgcattggtc aagttgcagc gcgtcttgcc gctgaccaga
     2341 atttttttgc cgcagtcatc ggttttttca aggagtaata cttttgcacc caattcaccg
     2401 gcacgaccgg ccgccatcat tccggcagct cctgcgccaa tcacaataat atcgttttcg
     2461 tttttttctt tttgcataaa gatcattcga ttttttaatg gggtaacatg aaagagtaga
     2521 aacggtcaat aattctatgc tgaggaataa atcctctccg atggggaatt attccccgca
     2581 tttctttaat aaaaaacatt catggcgtga cttaataaat ttaacttttg tattttcaga
     2641 tacatgcaac aaataaagac tgtctttgta ctggcatgta aattgcttta atgcgtgaac
     2701 gacaaacgta tccacctaac ttttaaagat agaaacaata ttccaactta aataaatgtg
     2761 tcctggcgac atgattattg aacttttgtt tgtgtccctc aatgagggtg atgttgcaat
     2821 aaaataaaaa ttgatcaagg aggaatttca aatgaaaaaa gttgtattgt tggttgttgc
     2881 attaatcttc tcattttcca tgacagttgc tgccgttgag aaagcagagg taccggacac
     2941 atcagagcca gttaaggttg ctgatattga aaaagatgac atcaagaaag gtgatgtaaa
     3001 aaaagacaaa accaagaaat caaagaaaaa aaacgtgaag aagtcaaagg ataaaaagtc
     3061 taagaaatca tcaaagaaag atgaaaaaat cgttgaagat gcggaatccg aagtacccgc
     3121 tgctcccgcc gcaaaataag ttagtaaaat ctttttctgg ttctaaagaa gggataggtt
     3181 gtgatctatc ccttcttttt agtgaagact ttttagaaat gagtactgcg aaatttgcag
     3241 agcaagtcga actgttcaaa agcctttggc ttttggaaac tattccctga atgaatagac
     3301 tcgggttttg aatcaaactg atagttggcc aatttgaact ttaggggatc ttgaggtgaa
     3361 ccatatcagg tttatagagc tatggtaaaa aaaatcccga cgaaacggga tgaagctctt
     3421 tcctgtcgtt ttcaagtttc tgtgttcaag ttttgtagat aattgttttt gtctttgata
     3481 taccctcgca tatggttaag acaattctga aaaagatcac cgctttcaac aacaggttga
     3541 cggtaagatc aaagttgctt gttcttattg tatctggtct tgcattgacc atgggtgttt
     3601 ggggaattat tcagctcgcc gctctggaaa gaatattagt tgaacagcag gcgaaaagat
     3661 tgaaagatgt cgctgaaaca gtcagtactt tttaccagga ttttccgacc aaaaaaggac
     3721 tgaaggccct tgaatccgct cttcatgatc atttacaggc tgacggtcgt ttggccagga
     3781 ttgacatctt cgatatcggt caaaaacatt ttgactatgt agccggagcg agtcgtgatc
     3841 cgtatcaatg ggcggataat attgtgatgt cagttatgaa gaaaaataag ctgcattata
     3901 tgaaaataat gacggaaaac ggaccggcgt tgggactgct ttatccggtt gctggaaaca
     3961 cgaaaagtgt gcgcattgta attggtatca tcagtttttc gcgggccaat gaagaaattg
     4021 tacatcgggc tggccggctt ttaattatca gcagcgccgg cctattaatt ttcattcttg
     4081 tagttcttgg cctgagttat cgctggatca ttggcaaacc attaaagtta attatccacg
     4141 ctatcgatga atttcaggaa ggtcaatatg ataatcgtat ccccattgtt catcgagacg
     4201 aatggggaca gatatccaga cactttaata tgatggctga tgagatccag gacgtcatgg
     4261 aaagaaatca ggaactcacc aaacatctgg aaaaacgggt tcatgaagaa accatcaagg
     4321 tgttccaact gcagaaacag gttacggatt tgaagcaatt aaccacgttg ggacaactga
     4381 cggccaatct ggcgcatgat ctggggacgc cccttcattc catcgctgga ttggcgaaac
     4441 tgcttttgga aaagggcggc ttgccggaaa acgtcgaaca taaactgaat ttgattgtcc
     4501 agcagacgct cagactggat gccgtgattc agaacgtgcg caaggccatt cggctgcccg
     4561 atcccagctt tgaagcactg cctgtggaac agatattcca tgaaatgctt tccctcatgg
     4621 agcctttctt gcaaaaaaac aacgttgaat ccgttgtcac tatcacgaat ccggtgtctc
     4681 ctctgtatgt ggaccgctat cgcattcaga cagctctcat gaatgttatg cagaatgctc
     4741 tggaggccat gccggaggga ggaacgatca ccatgtcggc acatgaggtt ccatgcgaaa
     4801 actttgtggc cattaccgtc agcgatacag gttacggaat gacgccggat gttgtggaaa
     4861 atgtctgtga gcctttctac agcacgaaaa ccagcaaagg tttgcgcggc ctcggcatgg
     4921 ctatcgtgcg tgacgtcatg aagatccacc ggggtcagat ggaaataaaa agttctcgcg
     4981 gccagggaac aatggttatt ctatgtttcc ccaaattgaa ggatgcagat cattttcatt
     5041 aaaaaaccgt cctggcgttg aaatgatttt tccccgctga agtgcttttc ctaagacaga
     5101 ctgtaacgac gtatctttgt ccggagagtc tttctgtcaa tgtccagccg ttttgccgcc
     5161 tgggactgat tccaggacgt ttccttcaac gtttgcagta ttttttcttt ttccgcttct
     5221 tcgagagggg ataatccggg accgtttagt atgtctttcc tgtctgtcga actatgaaaa
     5281 cgttccggca ggttctcggg gaggatttca tcaaaaggcg aaagaagcat aatttgctga
     5341 aggacatttt ccaactcccg aacattgccc ggccaatcgt aagccatgaa catctccatt
     5401 gcatccgggg aaacgcttac gtttgtatct cccaactttt tcagcaaggc ttccgtcaaa
     5461 agcggaatgt cttctttatg ttctcttaaa ggaggaatgc gcaaccgcac aataaatcgg
     5521 tataataaat cgaggcgaaa attctctttt ttcacctcgt catctatttt tttattggca
     5581 gcggcgacaa cacgcacctc gacattgcga acggcattgt cgccgatttt tcgaagttct
     5641 ccgttttgca tgaaacgcag aaatttaccc tgcaacgctg tggacatttc cgtaatttca
     5701 tccagaaata ccgtgccgcc ttcggcagtc tccagaagcc cggtgtgctg atggctggcg
     5761 cctgtgtatg cgcctttatt gtatccgaaa agttccgatt ccagcagatt atcgggaatg
     5821 gctccgcaat gaacgatgat gaagggcttt tcgcggcggg aggacatatt atgcaacgag
     5881 cgtgcggtca attcctttcc tgtgccgctt tctccttcga tcaggacgct ggcggaggaa
     5941 tcggcaatgc gggcgatttg tttatagaag tcgaccatca tggccgacga accgatgaat
     6001 tttgactctt cgacctttgc gggctgcagt ttcatcccct tgtgccgaag ttttcttaac
     6061 tccagcactt tacggacaag cgtgcggcag tcttcgggag aaaatggctt gctgatatag
     6121 tcccatgctc ccttgttgac agcggccatt gtggtttcca gagagccgta agcggtcatc
     6181 agtatcaccg gcagatccgg cgattgtttc tggagttctt ccagcaattg cagtccgtcc
     6241 ataccggaca tgcgaatatc ggacatgacc aaattatagg aggcaatatt ttgccggaga
     6301 gcttcccagg cggaactgca gagggtgacg tcgtaaccgt cacgtttcaa cgtttcttcg
     6361 agaaattcac acgccacagc atcatcatca acaacgagaa tacgtttctt cataaaacct
     6421 cttattcgtt cacatcgcga ggaggttctc cggtcacgac tgcgatgaac gatcccgaca
     6481 gttttttgct gcccagcaga ttctcggcga caattttaca ttcgaataat ttttcgccgt
     6541 tttcaacaaa tagtctgatc acttttcctt ttaccacgat ggttgccctg tcttttgtat
     6601 tttcaaaatt atcccagtca acgggttctg tcttagccaa aaaacgggag tggaattttt
     6661 ttatattttt gttcccgatc caggacgtga tggcttcccc tgcgatggcc atgataaaca
     6721 tgccgtgggc aatgacattc tgtaaaccga tggtgttggc aaatgtctga tcatgatgta
     6781 gcggattgaa atccccggat gctcctgcat agcgcaccag atgggttctg gtaatcggaa
     6841 gagaaatgta ttccggcatc tcgctcccga tttcaattgt attatgttgc ccggtcatct
     6901 ttctatattt ccgttaacgt tgtgcgggat tttgccacca actcattgcg ctgattgata
     6961 tattcaactt ccagaatagt catatcaaga gttctagccc ctttcccctt tttaatgata
     7021 tcggcaactt tcattttccg ggtgatgata tcacccgcat ggatagggca aaaatattcg
     7081 tactcttctt ccctgtgcag aagtcttttt aaattgattc ccagggcggt caccatgcct
     7141 gtcagtccgt cgcctgtcca gaaaaaagaa ctcgtcagga aagtgggagg tatgggaatg
     7201 ccttcataac cggctgcttt cgcggccttt tcatcatgat aaacaggatg aatctgattc
     7261 ttcatttctt tttgagaaac agcgatggca aattcaataa ttttgtcttt ctcaatgatg
     7321 aaagtggatg cgggaaattc cattcctatt ttggattgat ctgccataaa tacccctcaa
     7381 tatagcatat catatccgtt tcaatatcgt acagtccagt gaaaaattat aagaaaatga
     7441 gtgccctggt taaaaagaag gggtgttttt ttaatcttcc tcacggggaa gagtggttga
     7501 aaaattgaaa aatgcctgcg caagtacaac gcgcaggcat ttttcttaag caaggagaaa
     7561 aaattttatt tcagttgttt gctggaatat ccgagaacat ttctggccac aattagatgc
     7621 tgaatttgac cggtgccttc gaagatatcg gtaattttga catccctcat ccatttttca
     7681 gcgaggtttt cgcaggagta gcccaaaggt ccaagcagct cgcagcattt ctgacagatg
     7741 aacgtgccgt aacgccccgc tttagccttg gacatggacg cttccagatt gttgaattca
     7801 ccgttatcca tcatccaggc ggcacgccag gtgagcagcc tcatggcgtc caggtgggcc
     7861 tccatcaggt aaaactcttt ttccatcgca ctcagattat tcggattctt ttcataatca
     7921 agcgtgatgc cgcttgcttc cattttttct tgtgtaaaat ccaaagatgc ccgggttacg
     7981 ccaagcgcca tggacgcaac cagagggcgg gtgttatcga aggttttcat agcgcctttg
     8041 aagccttcgg ttgactgttt gacttccgcg cttcccagaa tgttgtcctt ggggatgcgg
     8101 caatcatcga gaacgtaagt gccagtgtcc gaggcgcgaa caccgagttt atgctccagt
     8161 ttttccagtt tgaatccggg gcgtcctttt tccaccacga atgattttat cccggctttg
     8221 cccgcgcttc tgtccaccgt tgcccagatg accgccactt cagcgcgttc agcgcatgtg
     8281 acaaatattt tttcgccgtt gagcacccac tcgtcaccct ccaaggtggc cctggccgtg
     8341 atggatccgg aatcggaacc ggaaccgggt tcggtaatag ccatggatgc ccatttttcg
     8401 ccgaaccttt ccatctgctc gtcggttgca accgccgcaa tcgctgcgtt tcccaatccc
     8461 gcgttcggca gtgacaacaa cagtcccagg tctccccagc acatctcttc gatgacgatg
     8521 accatcgaaa gattggcccc cagttttttg gcgcctggtt tgtccggttt tgtttctccg
     8581 ccctcttttt tctttttcgg acggctcatg atttttattg aacggaatac gtccagttct
     8641 ttagggtatt catgttcgtg ttcatcgtaa tgtcttgaaa cgggccgaag catgcttgac
     8701 gccatgttgt tcatgccgct ttttacatcc atcatctttt gtgtcatttc gagattaatc
     8761 atcgtcttac ctcctatacc gtggccatgc cttcaagaat ggcgatgcct ctgccgttgc
     8821 gatagtatcg ttccaccgga ttttcccgaa tgtaaccgtg tccgcccaga atctgcacac
     8881 cgaaatcggt cagtttcatg gtcatttcgc cggcgtagag ttttgccaga tacgcctctt
     8941 tctttgcgtc gcggccggct tcaagagccg atgcggcttt ccaggtcatc aacctcatgg
     9001 cgtccacctc ataggccatt tccgcaatca taaaagcgat ggcctgacgg ctggcaatcg
     9061 gttcaccgaa ctggatacgt tctttggcat attcccgcgc gtattcgtac gacgcgcgcg
     9121 aaacgccggt cccgatagcc gacatggcga tacgggtttt ctgcaggaat ctgctgtaat
     9181 cacatccgtt atctccgccg acacggtcat catcagggac ttcacaattt tccagcacga
     9241 ccggaaaact ttccagagca tacatcccca gatttttttc ccgctcgccg attttgagac
     9301 ccggattgtt cccttccaca ataaacaaat ccatcttccc gtcgatgtcg gctgccacca
     9361 gcatgtggcc ggaatccttg gccatcggga caaaacactt tttgccgttg atgatatatt
     9421 tgctgccgtt tttaacggct tttgtttcca gtgaaacggc gtcgaatctg aacagtggct
     9481 cattgatcgc tacggtacag gcttgatatg tcggagtgca gtacaacggc aaatacttct
     9541 ttttttgctc atcggtgccc atctgcaaaa tggtgtagat gaaggttgcg ggcagggttg
     9601 acgcgatcgc cagcgccatg tcgcctgcgg caagttcctc gacaaaaatg gcattcataa
     9661 tgggagaata ttccatgccg tatccgccgt attcttccgg tacagtggac accgttgctc
     9721 ccagttccca aaccttgtct atgatctccc ggggaagctt tctggcttca tccatatcat
     9781 gggcgggatt ccccgaaacc atactcttga ccaacttcgc aacagtatct ttcatactgc
     9841 tttgttcttc agacatgccg aacccaaaat ctctgtcttc cataatcatc tccttgaatg
     9901 cggtcatggg gcttgaacgt tacaagatta tcctgaaaaa attgaccgac tggttttcat
     9961 aaaaaatagc caggggtgtg ttatcccaat ccccccaacg gtttttcgac acacccccgg
    10021 caatggtgtt taacgcaaca ggttgatccg taatgagtaa cttcttctcc tcgttttaga
    10081 taagggtttg cagtttcatt agaagagtca tgtcgccggc gaatttcaac ttgccgttca
    10141 tgaaaagatt ctgtcctgtg gtttgcttat tctgcatggc aatccaatcc tgcaaatcaa
    10201 gactgatatc aacttttggc gcctcttccc cgttaaaaat catggtcacg ggaatctcat
    10261 ttccatcctg tttcagtttc agattcaaag tgcctttggt atccatcaga tttttaagcc
    10321 tcatgggatc ggcaatttcc acaggatccg tgaagcggtc aatcaaacct tctgcctttc
    10381 cggttacagc atcaagaaac acctgttctg aaagcgataa cgtgagcaga ggtttgtcta
    10441 cgcttccctt gacgacatcc agattcgtgc cgtctttaat tttcaggcaa tattgctgat
    10501 cgttgacatt gaactgaaga ttgacttctt tgcccgttaa agatgacaga ttcgctccgg
    10561 caatcacttc tttgaactgt tcggggacga gtttttcgaa aaagtcgttt acgctgacac
    10621 cgcttggagc tttaaatttt gccatagtcc atttacctcc atgaattttg ctataatatt
    10681 ttatgtggat tgttgtttac cggttggtaa gtactagaaa aaaaataacc tgtcaacttg
    10741 ttttttcatt tttgagtttg acgtcggatt ctattttgtt ataagttagg ataaacaata
    10801 acttacaaag gaatagagcg tgaaattgtt ttcggcgaac aggggtagaa taaaaaaata
    10861 aaaaatgaat atttttattt accatttggt ttggtaaatg atatgatcaa cgcatgaatg
    10921 atatagagaa aaaattgaaa tcatctttgc aaaaattaag taaaccttcg ggcagccgct
    10981 cttcatccaa tgagggcaat gtccgggagc ggattttaaa agcagcccgc gatgttttca
    11041 cggtctatcc cttcaaggaa gccggcaccc gcatgattgc caagcaggca ggtgttgagc
    11101 atcccatgat ccattattat tttggttcca aagaaaatct tttttacgcc atggccgaag
    11161 atttgtatca tgaaactctt tccgtgcagg aagcgtggct tgacggaatg gaaaacatgt
    11221 ctctgaaaga gggctttgag ctttttctcg atcgtatgct tgattacacc atgcaaaaac
    11281 ccgatgccct gcgtatcgcc gcgctgaatg tggtgaatat cggaaattta gaggaaatac
    11341 cgggctaccg tttcatcatc atgagtatgg cgtctatgcg gcgtatcatt gaagagaagc
    11401 tttcgttgaa aggatcaaat cgcgaaatcg aaatgtttat ttactgcttt tacagtctga
    11461 tgatctccct gatcggggca agatcatgtc aggcccagat attaaacatg aaccccctgg
    11521 aggagaaata ccgcacatgg gttaaggatg cgagcatgac gctcttttat ccgtggttta
    11581 aaaaaattct tgtcggccgc taacgcatcc tacagatatt gcagagactt tccggtttaa
    11641 tcttttcctg ccgttttacc tttcctggca gtaccggttt tcagggtgtg ccaatttagc
    11701 cttgacaaat aaattctact gtgatttacg cattacccta ttacttttag aggattcact
    11761 aatgaaaaag actttgacca ataaaacaaa taccaaaaga aagaaaacac acggttttct
    11821 cgtcagaatg gcatcaaaag ccggccggga ggtcttaaat cgtcgccggg cgaagggaag
    11881 aaagcgtatc gctgtttaaa ttcggcatat cagatgccat ttttaaggat ggtcgggtca
    11941 gtatgcatga aggaacaatc ttatcggaaa ctggaaagga taggcagccg aagcaggttt
    12001 aaaacaattt ataatcaggg ggtgtggaga agttcaagac attttacaat tgtaacatgc
    12061 ttaaacactc aaggaatcaa aagactggga attacggtca ctaagaaaac gggaaacgca
    12121 gtaaaacgaa acagaatcaa acgactagtc agagaatttt ttcgcctcaa taagaacctg
    12181 tttcccgaca attacgatgt cgtcatcatg gctaaacgaa acataccgtc cttgacgtat
    12241 aaagaagcat gcgatgaatt aacgggactc ttcacgcggc aaaacaatat ataatttatg
    12301 ctcagaaaga tattcattaa tttttttgtt tcatttattc atttgtatca agcctgcctg
    12361 tcacctttct tttttgctcc ctgctgccgg ttttatccct catgttccca atatgcgatt
    12421 gaggccatca aactgtacgg accggcaaaa ggttcctgga tgggcataaa aagaattctt
    12481 cgctgtcatc ccctgaatcc tggcggttac gatcccgtca agtaaaaaat agagaggttg
    12541 aaaatggata agagaaccat tctggcggtt gtcctgtcac tggctgtgct ttttgcatat
    12601 cagatttttt ttgccaaacc gcctgtgcca cccgaaacgg ttccggttcg ggaatcacat
    12661 cagacagcgc aaaaaccgac cccgataaag gaaaattctg tcgtcaagca acaagcggtt
    12721 tctcccgatg cctccacgac gaaacagaag gtcaaaaacg tccttccggc acaagaagaa
    12781 tcatcccggg acatcaaggt tgaaacggca aactacaccg ctgtgttttc caccaaaggc
    12841 gcggctctga agtcctttca acttaagggg tatcagaaag aatgcacaaa gtgtgtcggc
    12901 gatatctggc ccgcgatcaa gaattttgtt acgggtaaaa aacagccggc cggagaaaaa
    12961 aacaatgatt tgatcgaggt tgtgtcatta cagccggata tgccttatcc gcttacgctt
    13021 acttttcctg agtcatccgg cgagattgcc gcggattccg tctacaaaac ggacacagcc
    13081 gagttgaata tggttaagaa cagcaagaag cagcgtctgg tgttttcccg cgtgttcaat
    13141 gatgtcaggg tagaaaagat ttatatattc aatcccgata atttttcaat aacgctggag
    13201 gtcaacatat ccaatctgac cgatgtgccg ataacgaaaa tcccgtatct taactggtac
    13261 gactatgtgg acccgaaaga aaaagtagac ggctacagca aagaaggtcc tgtggcttac
    13321 gtgtcgaaat ccatcgaacg ctggaaatcg gcggatgtgt cgaaagaaca aaccatcggt
    13381 cccgatgtgt cctggggcgg cgttgaaaga aaatatttca tggcagtgat tattccgcag
    13441 gatccgtctt taacaagttt caacatgtcc cgggatgatc acgtgattgc cgtgaatctg
    13501 aaaggagcaa aaaatatcat cccgccggag cagtccggtt cgttcaaata taatctcttt
    13561 atcggtccca aggactacgc catgctcaaa aaactggggt tgtccctgga agaggccatt
    13621 gaattcgatt cggtcattcc gggattgaag tggctctcca tcggtctgtt gcttttcatc
    13681 aaatttattc atcagtttgt gggcaactac ggtgtggcta ttattattct gaccattctg
    13741 atcaaggttc ttttctggcc gctcggaaat atcagctaca aatccatgaa agagatgcag
    13801 aagattcagc cccaaatcac cgctttgagg gaaaaatata aaaacgatca ggccaaactg
    13861 ggacaggaaa caatggcgct ttacaaaacg cataaggtca atcctttcgg cggatgcctg
    13921 ccgatcctga tccagatccc cgtgttcatc gggctttaca acacactgct gtatgccatt
    13981 gaactgcgcc attctccatt tttcttctgg attcaggatc tatcggctaa agacccgtat
    14041 tacatcacac cgattgtcat gggggcgaca caatttattc agcagaaaat gacacccacg
    14101 atgggcgatc caatgcaggc caagatcatg ctggccatgc ccatcatttt cacttttatc
    14161 tttttgaatt tcccctcggg gctggtcatt tactggctgg tcaataatat cataagcatc
    14221 ggacagcaga tatacattaa taaaaaatta acggattgat tcaagcgccg gttggacaaa
    14281 agaggatgaa aaatgagttc tgaatttatt gaaatagaag aaaaaaccat tgatgaagcc
    14341 attgagaagg cctgtcagac gttcggcgtg cctcgcgaaa agctcaatat cgaaattatc
    14401 tcaggagaat ccggaggctt tctgggattg ttttccaaaa aagcgaaaat cagagcgtcc
    14461 ctgctggctc tggatatcga tttttccttc tcggataaac ccaaagaaaa aacggacaag
    14521 cccagggaaa aaaatgaacc ccggtccaaa gcggaccatt accgggaaaa aagcgaagca
    14581 aaggttttgc ccaaaaggaa gccggaaaaa gaagtaaaag agatatcgga agaaaaatcg
    14641 ccggtaaaaa aagagcggga ggctttcatt cccaattccc ccctgtcgct caaggcgcag
    14701 gaactgctgg agggcattct gcagagaata tcgccggaat acaaagcaaa tgttcaagaa
    14761 actcaggaaa agatcattct gggaatcgag ggcgacgaaa gcggcctgct gatcggcaaa
    14821 cgcggacaga atcttgatgc gttccagtac atcattaaca aggccatcaa taagatcgac
    14881 agcgaacaca agatgattct gatcgattcg ggagaatacc ggaagagaag agaagatttt
    14941 ctgcttgatc tggcggaaaa aataaagaaa aaagtcaaaa aaaccaaaaa gccggtatcc
    15001 atcgccaata tgaatgctca cgatcggcgc attattcatc tggtcatgca ggaggacgaa
    15061 tcactcgtga cgcaaagccg cggggaaggc aagtaccgga aaattatcat ccttccagca
    15121 aaaaacggca gcagccggcg cacgaaagag aaaaagaagg atatcaacca atcataataa
    15181 ggggaagtgc ctgccgtgct tcgcagcgat accatagccg ccgcggcaac gcctttcggc
    15241 atgtccggaa tagccgtcat ccgggtcagc ggaccggaag cccttgcagt ggcgcgccgt
    15301 attttcagac cgtcaaaccg tccatgcgac tggaaaagcc atcatcttta ccatggtgat
    15361 atcgtttgcg ccgatggcga aacggtactg gatgaaatcc tgatagcttt catgcgcaaa
    15421 ccccgctcat ttacgggcga ggatgttgtc gaaatcagct gtcacggaaa tcctgttatc
    15481 atccaatcca ttcttgacca attgcaaaaa ctgggatgcc ggctggcagg tcccggagaa
    15541 ttttcacaac gcgcttttct gaataaccgt tttgatttgt cccaggcgga agcgctggcc
    15601 tcgatgattg gcgcccaatc cgaaaaagcc tacgccgtgg ggctcgcaca gctcaaagga
    15661 tcgctttcca aagaaattga aacgatccgc gggttggtga tcgaagcgct cgccctcatg
    15721 gaagcaagca tcgattttgc agaagatacc gctgaagcag agcttgtgca aataccgccg
    15781 cagatcaatc aggcaatgga aagaatgcag aggcttctta catcctaccg ctttgccaga
    15841 gcctattcgg aaggtgttca tgtggtcatt acaggaaagc ccaacgtcgg gaaatccagt
    15901 ctgctcaatg ctcttgcggg aaagaaaaaa gcgatcgtca ctgatattcc gggaacaaca
    15961 cgcgatctga ttgcggatac gattcatatc ggcggtattc ccgtgcacct gacggatacc
    16021 gccggaatcc gggaaccgca aaacgtaatc gagcaggagg gtattgcgct cgtctggaaa
    16081 aaactggccg aagcggatat tgtcataatc atgctggacg tgagtaaacc gttgacagaa
    16141 gaagataaaa taacgattga taaaaacacg tcggagaaaa tcattgtcgc cgtcaataag
    16201 atcgacttgc cgcgttcgtg ggaaacgaaa agcattgcga acctttttcc gcctcataca
    16261 aagtttctgg aaatctcggc caagtccggc tccggactgg aagagctgaa aagaacaatc
    16321 ttggatttat ccgttgacgg tgaaacagaa agcacgtccg gggcgatcct gaccaattta
    16381 cgccacaaac atgccgtcga gcaatctctg aaaaatattc aaaacgcgaa agaaagcgtt
    16441 accgccggtc tatcagcgga atttatcgcg ttcgatttac gcgaagcgct ggatcatctg
    16501 gatgaaataa ccggcaaaaa aatcaacgac gagatcctgg ataggatttt ttccagtttc
    16561 tgtatcggaa aataatacgc tgtctttcgc gtcctagagc gttttgcaaa atcgttcttt
    16621 atgaaaacaa ggaccgcgga cggcggcgaa tttgtaatca ttataacatt atgatagtaa
    16681 aataacattc ggctttatac gcagtgacgc attaaagata cgaacttatg catggcatgc
    16741 ttaccgacat cggcatttct gttctggcgg cgaccgctct gggctttgta tttcagcttg
    16801 tccgtcagcc cgtcattctc ggttatctgg tcgccggggc gctgatcggg ccggcgatgg
    16861 gattcaatct ggtttccgaa gccgccagca tcgaggtcat ctccgagatc ggcctgatcc
    16921 tgctgctttt tatcatcggt ctggaactca atccgtccaa actcctggct tccggtcgtc
    16981 agctgatcta tgcgggtatc ggccagttta ttttctgcgt gctgatcggg ctgggatttt
    17041 ttctgctgct tggttacgaa ttgagccgaa acaatattga cgccctttat ctggccctgt
    17101 tttgcgctct gagttcgacg gcgattgtcg ttaaacttct ttatgacaag tttgaactgg
    17161 acaccttccc cggacggatc acgctgggca ttcttatttt tcaggacctg tgggccattc
    17221 tgattctggc gcttcagccg catttcacga atccgcaatt tcttctggtt tttatcgctc
    17281 tcggcaagag cgtggctctg ctggccatcg gttttttgct cagcaagtac ctgttgagct
    17341 ggatttttaa aaaaatatca caaacgccgg aaatggtggt ggccatgtct atggcgtggt
    17401 gcgctttgat ggcgggtatc ggttcatgga tcggtctttc gatggaaatg ggcgctctga
    17461 ttgccggtgt cgcgatttcg acgtttccct atagcatgca tgtgacggcg aaagttcttc
    17521 ccctgcgcga cttttttctg acccttttct ttctgtccat tggtatgaaa attccttttc
    17581 ccgatgccgc gattctgggc atggcgttga tcattgtcct atttgtgatt gtttctcgtt
    17641 ttttgacgat ttatccgctg ctgacgctgg cgggcagcgg caggcggacc agtttcatcg
    17701 ccagtctgaa tctcgcccag atcagcgaat tttctctggt ggtggccgcg ctcggcgtgc
    17761 attacgggca cattgatcag cggatgatgt cgttgattat ctacgccatg gccatgacgt
    17821 ctgttctttc ttcatatttt atcaagggga atcacgcggc gtatctggta ttcaaccgtt
    17881 ttctggagaa aaggggattt ccggccagga cgaaagaaaa tcatgatgaa gaggaaaaaa
    17941 atgtctaccc catcgtgatt ctgggattcc accgcggcgc gcgggaattt atcggccggc
    18001 tggagacgga gaccccggat ttattgaaaa agcttctggt gattgatttc aatcttgaaa
    18061 atttaaaaga attgagcaat aaaaacataa aagggatttt cggcgatatc ggcagcatgg
    18121 atacgctgga gcacgctcat ataaccgcag cacaaataat tttttcaacg attcccgaca
    18181 tgctcctccg gggaaccagc aacaaggctc tggtgacttc atgccgtgcc ttggcgccca
    18241 gcgcctacat cgttgctacg gctgattcgg ccgatcagat tgatgtcttg aggaaaagcg
    18301 gggcggatga ggtgctgctg ccttatgtcc tgattggaga aaaactgacg aggtttgttg
    18361 agcagacctg cctgacggcg gagtcctgca aactctaaaa cgtaaccggt gtagatatct
    18421 gtttatgaaa tggatgggta aaaaagttgg actggtactg ggcggagggg gcgtccgcgg
    18481 tttttctcat atcggcgttc tcaaggttct ggaagaagaa ggcatcgaca ttgatctgat
    18541 tgtaggaacc agcgccggtg ctttgatcgg aggcgcttat gcttccggcc aggctcccga
    18601 tgagatcgag agcaaaatcg acgcttatct gcaaagtcct gagttcgacg actccaggat
    18661 gaaatccatt ggactgtctt ttacacaaga ggagaaaaac attctcaaga aagcacaaca
    18721 atttatcatg aatcgcgttc tttttgttca ggcctttttc aaaccgtcca ttctgccttc
    18781 ccatgacttc ctctctctga tgaactattt tctgccggat ctcgatatta aagaaacacg
    18841 aattcctctt cacgtggttt ccaccgatct gatctccggt aaaaaaatcg ttttttccga
    18901 aggatcgttg cgcaaggttg tgctggccag ttgtgcggtt ccgggagccg tcgagccggt
    18961 tcgttacgga gactggctgc tggctgacgg cggcattacc agtctggtgc cggttcacgc
    19021 cgcacgggag gccggagccg acgtcgtcat cgctgtgacg gtggatcgag atttgcaaac
    19081 gaatctcagg atcgaaacgg cgaaggacgt cgttttcagg gcgggagaaa tcacccccag
    19141 cgttctggag gcggcggaat tggcggatgc cgacgtgatc atccgtcccc aggtcggcaa
    19201 tcttcactgg atggatttca aacgggccgt ggaccttgtc aaggcgggag aaagcgccgc
    19261 ccgcgattcc ctgaaaagca ttcacgcttc tctgccaatt tatcggcgca ttacacgctt
    19321 ggctggaaaa ttatttaaac aatcacggct ctaaacccga tttccggttt acgttttttt
    19381 agattgaacc ggaacggttt ttcaattatc ttggacaggc aatgtgcttg attcagctta
    19441 aaggaaggac ggagattatc atttaattat ctgattttga acatcatttt attaaggtca
    19501 tttagttctt gatttctgtt ttggtttggt atagataacg cgcctactat ttttaggact
    19561 ccttgcccgt ccaatcgggc tttagagccg aaaggagaaa acattgcaaa gatatgaagt
    19621 tgtggctatc gtcctggccg atttaattga ggacgagatc aacgcgttaa tcgaaagaag
    19681 tcagaccatc attaccgacc gcaaaggtgt cattgccaag gttgacaagt ggggcaaacg
    19741 gcatctcgcc tatgaaatca aaaaacagag agacggttat tattttctca tcgattttgc
    19801 cggcaacggt tccattgtca acgagattga aagaaattac aagatcgatg atcgggtcat
    19861 taaattcatg acggtgaaga aggaaggtgc gaccactgcc gaaggtattc agcaggaagc
    19921 cgccgcagcc gaagcaaagc gcgttcaggc caaggtagag gcggaagccg ctgctgccga
    19981 aggcaaacag attaatcaaa ttgagaaggc gccgcgggaa tccagaccgc gcgttagaaa
    20041 agatcacaac aaagaagaaa ctcaaacgaa gggggaataa gccatggcaa tggaaacaca
    20101 aaacggagaa aaacaaggaa gatatcccca gaagaagttt tttcacagaa aaaaattctg
    20161 caggttctgc tcggattcca atattaagat tgattataaa aatactgcga ttcttaataa
    20221 tttcgtaacg gaacggggca aaatcatgcc gcgccgcatc accggcaact gcgcttccca
    20281 tcagcgggaa ctggcactgg ccattaaacg cgcccggcag attgcgatta tgccttatgt
    20341 gacggctgac ggtagataaa tctgttccgc agtttttttg tcgttagaat aatggtgtga
    20401 tgattatttt tcaaaactga gtcattcgtt tcggtttaaa atcaattcaa caacatttca
    20461 gtcaggtaac gccgcgggcg gcgttacctt ctttttcaga aaaagcgacg gttaacgggg
    20521 tgttgcatcg tgagcagttt ttctttcttt aaaaaatccg gagcacccgc gtccggcaat
    20581 tctttttccg gtctcctgtc tgctgttggt ctgtttgctt ttgccgccgt ttttgttccg
    20641 cttgtcggcc ccgtttttct ttttctgctt ccgatgattg ccttcgtcaa tggagcaacc
    20701 aacggtatct ttaaaacagc actgtgtctt ttattttctt tagcggttct tatgctttta
    20761 tccgtcgggc tgaatctgga tgtgccggct gtcgcgattt ttaccatggg aatggccgga
    20821 ttgcttatgg cgcacatggc ggctgaaaat tactccatcg aaaaaatagt tacgggaccg
    20881 gttttattga tttttggagc gattggtttt tatttcgttt acgatgctgt gggcctgggg
    20941 gtgaatccct ggctgctggt gaagaatttt ataaccgtga ccatcagcga aacaatcaaa
    21001 ttttacagcc agatgccttt aaaaccggac gatatcagca tgatcaaaga taatgaaaac
    21061 aatatcatca acagcctcat ccacattttc ccgtccctga tggtcattct gtccgtattc
    21121 atcatctggg ctaatctttt actgggcaaa acttatctga gacgattcgg catcgtttat
    21181 gcgcaaatgg caatactggc gcgttggaaa gtgaccgact gggccatatg gatttttatc
    21241 atatccggcc tgctattgtt cattccacaa aagaacttta atttcattgg gttaaatgtt
    21301 tttctgatcg tctgcttggt gtatctgctg caagggctgg cgattgtcag ctttctcttc
    21361 cagaacaaaa acgtaccttt tttaatccgc tatatttttt attttttaat tgcagtgcag
    21421 caaattctta tgatacctat tgcggtcatc gggctgtttg atgtatggtt tgatttccgc
    21481 aagcgttttc aaaaaaatca tttatgaaca taatgatcgc aatactattt ttataaaaac
    21541 ggaggatttt catgaaggtc attctgaaac aaagtgtgcg ttccctgggc aaagccggaa
    21601 cggtggtgaa agtcaatgac gggtacgccc gtaatttttt aattcccaag ggactggcga
    21661 ttgaagccga tgaaaaaaac atcagactct tcgaacatga aaaaaacaat attctgaaga
    21721 aggccgcaaa agaacataag aacgcgcagg atctggctga tgttctgtcc cgcgaaacca
    21781 ttaccattgc ccgcaaggtc ggtgatcagg acaaactttt cggttcggta agcgcaaagg
    21841 atatcgaggc cgcgttaaaa gaaaaaggat acgacattaa ccggaagatg atcatgcatg
    21901 agcacggcga gcacattaaa gaactcggcg agttcgcggt caaaataaaa ctggctcatg
    21961 atgttgaggc acagatcacg ttgaaggttg tcggcgaatc ataatgaaag aaaaagatcc
    22021 atcgcaatat aaactcccgc cgcagaatct cgaggccgaa cagtctgtcc tgggcagtat
    22081 tctgctggag aacagtgcca ccaacagggt cttcgaaatt ttatccgaga gggatttcta
    22141 ctccgaggct catcggaaga ttttcattgt gatcaaggat ctgtccgaaa aaaacgaacc
    22201 ggttgatctg attaccctga gcaatgccct caaagataaa agccttctgg attctgtcgg
    22261 cggtacaacc tacctggctt ctctggtgga caacgttcca tcggcggcca atgccgccaa
    22321 ttatgcgaaa attgtcaaag agaaggcgat tttacgcggt ctgatcacgt cggcaacgga
    22381 aattatcgac aactgttacg aaaccggttc cgatgtggat actattctgg ataaggccga
    22441 acacagcatt ttcgaaattt ccgaaaacaa ggtgcgccag gattattttc cgatccggga
    22501 tattattaaa gacagtttcc gctctatcga agatttgtat tcccgccagc agttgattac
    22561 cggcgtgccg accggcttcg gaaagattga tgatttgacc gccggcttgc agaaatccga
    22621 actgattatt attgcaggac gtcccagcat gggcaagacg gcgtttgccc tgaatatcgc
    22681 actgcacgcc gcaatggaag cgcagattcc gactgccgtc ttttcgctgg aaatgtccaa
    22741 ggaacagctt gcttttcgtc ttctgtcctc tgaggccagg gtggatgccc agcgcttacg
    22801 aaaaggtttt ctgggagaaa cagactggcc gaaactgacg actgccgccg gccgcctttc
    22861 ggacgcgcct ctttacatcg atgacacgcc cgctattacc gttctagaaa tgaaggccaa
    22921 gtcccggagg ctcaaagcgg acaagggctt gggactggtt gttgtggatt acattcagct
    22981 gatgcgctcc agttacagtc acgattcacg ggaacgggag atatcggata tcagccggtc
    23041 cctgaaggcg ctggccaaag aactcaatgt gccggttgtg gcgctttcac agctgaaccg
    23101 tcaggtcgaa agccgcacca accgccggcc gcagatggcg gatttgcgcg agtcaggcgc
    23161 cattgaacag gatgccgacg tcatcgcctt tatttaccgg gatgaagtgt acaacaaatc
    23221 ggaggacaat atcgacaaag gtattgcgga aatcatcatt gccaaacaga gaaacggccc
    23281 caccgacacc gtcaagctgg cctttgtgga caaatacaca agtttcgaaa atctgtcacg
    23341 ggtggaactg cctgaaggac agcattgatc cgaatatccg gtcgtgcatc accggccaat
    23401 gaatgccctc ccattttccc
//
LOCUS       DAONQZ010000034        23370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2441_length_23370_cov_14.886725, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000034 DAONQZ010000000
VERSION     DAONQZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23370
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2441_length_23370_cov_14.886725"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /gene="mnmG"
                     /locus_tag="PLR13_07000"
     CDS             complement(<1..1258)
                     /gene="mnmG"
                     /locus_tag="PLR13_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQC19122.1"
                     /translation="MTNLTDNYDVAIVGGGHAGCEAALACARMGCRTILFNINLDSIA
                     LMSCNPAIGGLAKGQLVKEIDALGGEMGKITDKTAVHFRLLNASKGPAVHSSRMQCDK
                     QLYRLTMKAVVENQPNLYVRQAMIEKLVIEDGKVIGVLDQSEHFYGAKKIIITTGTFL
                     NGLVHVGPSQTPSGRAGELASIGLANHLKELGFDIGRMKTGTPPRLRASSIDFSRLER
                     QDSDPDPRPFSFTTEKLRTERLPSYFSATSAETHRVIRENIQHSPLYSGIIKGVSARY
                     CPSLEDKVMRFAERQSHPVVLEHEGLDTQEVYAKGLGNCLPLELQYKIVRSVKGLEQA
                     QIMRPAYAIEYDFVQPTQLKPTLETKLVDGLYLAGQINGTSGYEEAAAQGLWAGINAA
                     CAVQGAPAFLLDRSEAYMAVMIDDLVT"
     gene            1459..2412
                     /locus_tag="PLR13_07005"
     CDS             1459..2412
                     /locus_tag="PLR13_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HQC19123.1"
                     /translation="MPKPDFWKNNKNYYDLKSYWRNLFGCSVHKLQIDAGFTCPNRDG
                     HVAMGGCIYCDGRGSKLRQAGELPSVTEQIASGKKYYTKQAGKFIAYFQTFTNTYAPV
                     EKLRALYDEALNQEDVIGLSIGTRPDCIGPHVVELLSGYAQKYHVWVELGLQSVHDKT
                     LTLINRGHSFQQFEDAVNALAGHGLNICVHIIIGLPGESDEDVLATAKTLAAMPIHGI
                     KIHSLLALDGTALGEMYKKGRINMITKEKYVSLVADVLEVLPPQTVIQRLTADGYRDI
                     FLAPDWAMNKLDVLNSIDKELIRRDSYQGKKYRTNRAESLD"
     gene            complement(2453..2659)
                     /locus_tag="PLR13_07010"
     CDS             complement(2453..2659)
                     /locus_tag="PLR13_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19124.1"
                     /translation="MEEKERMIDRLFNPMLDALEDHPYSGMLVIAFVCILIVAFFPPS
                     LVFILIFIGYGFYLNSKRSKKDKP"
     gene            complement(3017..3790)
                     /locus_tag="PLR13_07015"
     CDS             complement(3017..3790)
                     /locus_tag="PLR13_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19125.1"
                     /translation="MMKKMFLSSLVVFIAASIVSSPPVCFSEQPDPKVWKHLSDDFYY
                     NKINLIQSADSISVWTYRTIADDERKYLIEHFRESDPKKSIKYQKLDHQTILLKMDCR
                     NKQSKIEKLVNYDDNESVLYEETYNNSEWTGIIPGSKPEEIYHDICVTSERQEQLHAL
                     KMQCEKDCDAWVRVDENKFFRDPFTHQSHYNTRLDKCFILLNYTKKPLKVLREMKGNK
                     IYGAVRSQKNGKTITCNVLEKTCKSEAEWDAHVKPYMEE"
     gene            complement(4002..5819)
                     /locus_tag="PLR13_07020"
     CDS             complement(4002..5819)
                     /locus_tag="PLR13_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC19126.1"
                     /translation="MTENDIKTLPQGLKWLVDKQPDRVAMRMKDYGIWHDISWRQYYE
                     NVKKVAVGLYALGVKPFDHVAIVGENKPEWLFSAMGVMSLGGIFVGVYTTNPTAECEY
                     VVGHSESVVFFCEDEEQFDKALAFRERTPRLKKIIVWDMEGLKHFDDPMLMSFEKLLE
                     LGEKIDKERPGLYEKIVAGRESEDTASIIYTSGTTGPPKGAQISHQNYLWIARQSQKI
                     TKLSVDDETISFLPLNHVYEQIFDLMMHLTTGHIVNFTENTDTVMNDLRDVSPTLFHA
                     VPRIWEKYHSGIVLKMADATWLKRTVFNLAFKIGTTYNGFKLAGKPIPWYLFLGYQLA
                     YFSVFWKLKQRLGFDKVRLGFSGAAPISHEVLKFFQSIGIPIREGYGLTETTGITHMS
                     DEEHFKVGTVGRALPDSEVKIAEDGEILIKHGGIFQGYFKEPEATAEALEGGWFHTGD
                     VGEIDAEGYLKITDRKKDLIITAGGKNIAPQYIENLLKFSPYITDAVVIGDRRKYITA
                     LIVIDEENVVKYAQDHKIQYTTFASLTRAEEVIRLISDEVEKANRQIARVENIRKFHL
                     LDKKLYTEDGEVTPTMKVKRKSISAQYKDIIEAMYTEDP"
     gene            complement(5829..6599)
                     /locus_tag="PLR13_07025"
     CDS             complement(5829..6599)
                     /locus_tag="PLR13_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19127.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIKEGSIVALLGSNGAGKTTTL
                     KTIMHLLDDDQPEKGSIEFLGKRIDRMDTEEIVRLGISYVPEGREVFPELTVMENILM
                     GAYTRKDRKGIQDDIDGILKQFPILSERKNQQAGHLSGGEQQMLAIGRALMSRPKLLM
                     LDEPSLGLSPLLTKEIFSIIQNINQKGVTIFLVEQNVNMALKYSHYAFLMENGRIVRA
                     DRPEILREDDDVKEFYLGIATETSVKGFKRYRRKVRFR"
     gene            complement(6599..7372)
                     /locus_tag="PLR13_07030"
     CDS             complement(6599..7372)
                     /locus_tag="PLR13_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19128.1"
                     /translation="MAHFKVENLNISFGGIRAVSDVSFEVEYNKIFSIIGPNGSGKTT
                     IFNIISGIYKPHAGEVFLDNENLVGLRPDTIARKGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGITSGAFMWGKRSFAAKEELKHREIVEHIIDFLDLQSLRDLFVSSLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYKVTILLIEHDMNMV
                     MGISDRILAINQGVKIIEGTPQDVQKNPEVIAAYLGEEE"
     gene            complement(7458..8648)
                     /locus_tag="PLR13_07035"
     CDS             complement(7458..8648)
                     /locus_tag="PLR13_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQC19129.1"
                     /translation="MKRFIIALTIVIAVFIASLLVFAQAKSGFDNKEIRIAQWGPQTG
                     PAAPWGSVARGSGLLFQIVNDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIF
                     AFVGGVGAACGMAVKDYLAQNKIIWVNPSTAIDEYVFPVNPYLFAMYTLYDDEASILT
                     KYTVEKLKAKKIGFLYQNDAYGKSGLKGCKARLNTYKMKLVEEISVEPGEKDLSSQML
                     RLKNSGAEVTFLWVNPTSAAIALKTAATIGFKPQWISSDTLMDFPLMNKITGGLWEGV
                     ITGGIGLPTDVDHPLMNKYRAAAKKYAPEERFGVFYTAGIMFAEPLVDALKRVGPKLS
                     TEALLRELNKTKNFQGLGAVVNWSPTVHQGCDSIQILKCGPNSSYIILQDWTSNELAT
                     WKKK"
     gene            complement(8717..9166)
                     /locus_tag="PLR13_07040"
     CDS             complement(8717..9166)
                     /locus_tag="PLR13_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19130.1"
                     /translation="MSKTVKILILACAVLVVFGLVIWDAPRNRLELFLEKSVIEETAR
                     NYLTAEMKKDSRQVYALLAPSSDYKKTHTYEEYLKDIAENSSAALNTCKIVRIYHLRD
                     NHNRHNYPAVDKLVQVEVEVTFGHAGPNSIFNYSFTFLKEKGKWYKG"
     gene            complement(9189..10061)
                     /locus_tag="PLR13_07045"
     CDS             complement(9189..10061)
                     /locus_tag="PLR13_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC19131.1"
                     /translation="MFEMLLIEGIAIGACYGLFGVAVAIIYKTSDVINFAQGEMGMVA
                     TFVAYHVLTVYNVPFWLAFPVTLIFAILLGMAIEFCFLRPAKDPTVLGLLIITLGAEM
                     LLMGLAGWKWGAEQKDFPFLLSVTKTHEMLPGIFINEWTIGVFSLSIIIMISLFLFFR
                     YSKLGIAMRATQQNVNAAKIMGIKVERVFSITWAMSSLIGAVAAMFFAARSVLDPNFM
                     MEPFMRAFAAAVLGGLTSIPGVVVGGLILGIIENFFGYAWPEWKPIVAFVVIILIITV
                     RPSGLFAKHYIKKV"
     gene            complement(10083..11153)
                     /locus_tag="PLR13_07050"
     CDS             complement(10083..11153)
                     /locus_tag="PLR13_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC19132.1"
                     /translation="MDMKRDYYEDVELFRSGTILTWSIVLLVFLFTLPVYLPSYNIYL
                     LNIIMVNIILAVGLNILVGYTGQISLGHAGFFAIGAYGTSLIMMNFHFPFFAAIILAA
                     LIAAFFGFIIGLPALRLEGPYLAIATLGFGLTIMHIIGRWDFFGGRMGITAPPLDMGI
                     SGTSFSYVIQGDVKIYYLILMMTVLLVIGARNLMKTRVGRAFMSIRDDDIAAEVMGVN
                     LTIYKTLAFAVSAFYAGVAGGLYGFVVGFFDPFTFNLILSIIFLVMITVGGLGSIMGA
                     IMGAMLITYLQYDLLKNVENLPYLGALLTTVSKKWFTVIGLANFSSIALGLIMITIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            11381..12007
                     /gene="hisH"
                     /locus_tag="PLR13_07055"
     CDS             11381..12007
                     /gene="hisH"
                     /locus_tag="PLR13_07055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQC19133.1"
                     /translation="MIAIIDYNAGNITSVARALQSIGQEFIITDDAKKLDAASRVIFP
                     GVGAAGEAMAYLRQKKLDVWLRDYIKTGKPLLGICLGTQIILDYSEENDTPCVSLVAG
                     STRRFPETLAFNGELLKIPHMGWNSVRLLRSHPVFDHVSPEAEFYFVHSYYPAPADEA
                     AILGVTEYGITFCSVLARNNLVAMQFHPEKSGRPGLQILKNFCTWRPS"
     gene            12004..12783
                     /gene="hisF"
                     /locus_tag="PLR13_07060"
     CDS             12004..12783
                     /gene="hisF"
                     /locus_tag="PLR13_07060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQC19134.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYAQGA
                     DEIVFYDITASSDKRNIMIDVVRRVAETIFIPFSVGGGIRNLDDMRNVIHAGAEKVSV
                     NSAAVENPQIITQGAKAFGSQCVVLGMDVKQVDVSDAIPSGYEIVVHGGRIYTGIDAL
                     WWATEAEKLGAGEICLNSIDADGVQTGYELNLTRLISENVHIPVIASGGAGKPEHLAD
                     VLTKGKADAALIASMVHYRTYTIAEIKHYLSAKQIKTRLFW"
     gene            12862..13743
                     /gene="pdxS"
                     /locus_tag="PLR13_07065"
     CDS             12862..13743
                     /gene="pdxS"
                     /locus_tag="PLR13_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQC19135.1"
                     /translation="MDKKRYELNVQLAQMLKGGVIMDVTNVEQAKIAEEAGAVAVMAL
                     ERVPADIRKDGGVARMSDPAMIAAIKRAVSIPVMAKARIGHFVEAQILEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRAMNREIGQLVQMPVEEVTGFCKTNGIPYEHALKVKELSRLPVVNFAAGGIATPA
                     DAALMMQLGCDGIFVGSGIFKSADPARRARAIVKATAYFNDPQKVLEASMDLGEAMPG
                     LEISQIPPEQILAGRGW"
     gene            13750..14439
                     /gene="pdxT"
                     /locus_tag="PLR13_07070"
     CDS             13750..14439
                     /gene="pdxT"
                     /locus_tag="PLR13_07070"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HQC19136.1"
                     /translation="MSLRLVSDKPSVIGVLDLQGGVHEHLEHLERLGVACKRVKQTED
                     FKDLAGLIIPGGESSCLSRLLTIFGMKDVLLAAHRRGMKIWGTCAGAILLAQNVVGED
                     ACLKLIDITVERNSFGSQLDSFAGEALIPGISADPVPLTFIRAPKILAAQPDVRVLLK
                     IDDYIAAAENDSVLVTVFHPELTGCLALHQYFAVKCGLKTATGQAGHSDPDWDISSWT
                     KYAALAGRAGD"
     gene            14562..15668
                     /locus_tag="PLR13_07075"
     CDS             14562..15668
                     /locus_tag="PLR13_07075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQC19137.1"
                     /translation="MQNIFSELKTDSRFIRFVMKVGSPSPMDPDEQHRRIPAVFVLMI
                     ISVATAVSGIYHCSSGKNPLVVLDVIGFILSLIMLLYLRQGKNPGIAYGMISTGFVLL
                     CCAATVLGRTEMSLIFWALVLPAGCFSILGRKMGMFVAAFFFFINLLLMNLPEHMILS
                     KPFPASVAARFSVVYLILTFMLYYYESSHQMLIRYIRHEENKFQNASRYDPLTGLSNR
                     RDILEKMENERERHSRLEKTFTLIMGDIDNFKMLNDTLGHEAGDYVLQTIGRILKNQV
                     RGIDCSSRWGDDEFLILLVDTDLEGGQRVAERIRKKVENTSFQYQGKGLAVTMTFGLS
                     IYQSADEQIDACVRRSDDALEEGKRQGKNRVVPV"
     gene            complement(15729..17093)
                     /gene="mgtE"
                     /locus_tag="PLR13_07080"
     CDS             complement(15729..17093)
                     /gene="mgtE"
                     /locus_tag="PLR13_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQC19138.1"
                     /translation="MQKVKKKEPTALETLKDLISRNREFDIIDLFSDMHPADIADLID
                     ELEEEDRLHLFSLLDVDKASDVILELSDTSREQLIEDLSNEKLTDIIEEMDSDDKADI
                     IAELSDDQAKAVLEAIEPEESQEVQELLKYADDTAGGIMQSELVSVRKDATINDAFQA
                     VADAKDEIENIHNIFVVDETDKLIGTVPLQSIITTKRFTPILDIIDEDIPSVLHDMDQ
                     EEVARLFKKYDLVSIGVVDNQQRLLGRITIDDVVDAIQEETSEDIYRIAGLGEDDTVF
                     NNTTESVKKRLPWLYLNLITALASVLVIGFFEETIKLMVALVFFMPVVAGLGGNAGSQ
                     TLTLIVRGMALGEITFENAKRALFRQIAVGIANGIAVGIVIGIIAYFWKGIPILGLIL
                     GLAMIISIFTGTLVGALIPLALKRLKLDPALGSHIFLTAFTDAFGFLTFLGLATIFLK
                     IFHL"
     gene            17388..18218
                     /locus_tag="PLR13_07085"
     CDS             17388..18218
                     /locus_tag="PLR13_07085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19139.1"
                     /translation="MTCLYSLREFYFLGAKGTILIKADNIEKNFFSVGRYWGNLNASL
                     KRKESVWWMNTGVAVSDMNWAWNETPLAAPDTAIVSEIREDYKKNNLRFWWWVYPCGQ
                     SAATSAILQNAGFRLYANVCCMAADLNESFPEPYATGDMEIYHVRSREDLAVWEDVAF
                     HGFEMPRHARDPFGAFVSSFETGDQFPQRFILGCIQGKPVATSLLFKDQRTAGIYYVS
                     TLPSYRNRGCGLSVTQAAMQLARESGFKHVVLQATPMGKRVYERLNFKRYYHAGIYTL
                     "
     gene            18356..19246
                     /locus_tag="PLR13_07090"
     CDS             18356..19246
                     /locus_tag="PLR13_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19140.1"
                     /translation="MKRNSGHNIACDNECGGLHQSNPLSLAKNEISSTLSWFMTDNAL
                     YPGVDKHSSPLRYENWSGRYSEYISRQEAESIVSRFICPEKDENFTLFFVSDYKDTYL
                     GKNFLKDGSDIYLKHYRMTDAPREIRLRNVLRRCLARKNFNAFYQLHQKNIEAVKPLF
                     YVMKRGRWIPDEVIAVSKGASGNKTIETLMADSRDNDEFRLLAVNLGSYVAELQSREI
                     LFKELYRNLIAVNQTTHWDFILCDLDEIRSVSEKRFYKHRKHVEKLRKKIKRYGDSFL
                     HAFDQGYQTRMHDQTAPSRR"
     gene            complement(19306..19650)
                     /locus_tag="PLR13_07095"
     CDS             complement(19306..19650)
                     /locus_tag="PLR13_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HQC19141.1"
                     /translation="MGKMTQCPWGSFEVLSETAGHKVKRIIVEPGGGLSLQLHHFRSE
                     HWFVVAGTGDVTVGDKIISLSAGGSVDIPAKVKHRVLNHADGHLVIIEVQTGARCDDG
                     DIERFEDLYGRI"
     gene            19851..20489
                     /locus_tag="PLR13_07100"
     CDS             19851..20489
                     /locus_tag="PLR13_07100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQC19142.1"
                     /translation="MSLQVCDDAKIVVSAPSFTPADDIRRFVEEKQGWVAKILARQKQ
                     EAAHAKEKKYETGEQFFYLGQLYPLEVFFEPFEKEGIAFRNHRFYLNCRSDKVLRKQC
                     FVAWYSKKADEMIRRRVDFFSGMLKLPYERIRITSAGSRWGSCSEDNSLSFSYRLIMA
                     PPDVVDYVIVHELVHIREKNHSSTFWQKVEAVMPSFREQRRWLQDNHSNLAL"
     gene            20523..21380
                     /locus_tag="PLR13_07105"
     CDS             20523..21380
                     /locus_tag="PLR13_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HQC19143.1"
                     /translation="MEHIEIIDLKHRDGLAERFKKMNLRISDYTFANVYLFRNISHYE
                     FVTKECGLFVSGWNKQNQNYVMPLNDPRGCDVKTFAEILGARNFFFPIPEEWLVYFPK
                     DKFSVSYDESESDYLYLTENMAGFKGKQYLRHRNHLKQFLASYIPDGVTLSSHNCNDA
                     MIVLHQWQEDSRTASDKTDFVQCSEALQNFSGLALWGIIYYIAGKPAGFIIGEALAGD
                     TFCLHFAKASKRYHGIYEYMFNDTAKRLESAYKYLNLEEDMGNKNLRRTKSSYGPEFL
                     LKKYRVGII"
     gene            21381..>23370
                     /gene="feoB"
                     /locus_tag="PLR13_07110"
     CDS             21381..>23370
                     /gene="feoB"
                     /locus_tag="PLR13_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQC19144.1"
                     /translation="MEKKTAMSESVNITSLQEGDQGVIHAIEGGSAITSRFAGMGIVS
                     NARFRIAQISGGLIVLQVANTRIALGRGEASKITVSKVAPEADVCMPPIEKEISVALV
                     GQPNVGKSTVFNILTGLSQHVGNWPGKTVEKKEGTHRADSVLIRIVDLPGTYSLTAFS
                     EEERITRDFIIKEQPGLVVLVLNATALERGLYLLAEVLLLNRPVIVVLNMLDVAADQD
                     IEINIRALEDSLGVPVIPMVAKRNSGIRELVSHISSIASSEYKFQPVKPEVSTDHQLI
                     YQQILTLVRPYIHDPYTPEWVAVKLMEGDPEVTQLVEQQVQTIARDQIEKLLIKHEDA
                     LHAVVNGRYDWIEEITRSVVSRFKMGQVVLTDRIDHVLTRPVFGIPILLAVMALVFFL
                     TYSMGVPLQEWLSDRIQQFIGFAEPLTTVWPSWLSGLLLNGVIGGVGSVLTFLPVLLI
                     FFAIMAFLEDVGYMARAAFVMDKIMHLVGLHGKSFIPMCLGFGCNVPAVLGARIIETK
                     KARMITLLLIPFVPCTARLAVLTLVSAAIFGQNGAFVAWSLVALNIAVLGIAGVFVNK
                     TLWKQDAPFIMELPIYHTPDAKTIMMVVWTRILSFIRKAGTVILAVSLIVWMLSYFPN
                     GNVEESLLASFGRLLQPLGIPLGLDWKMITALLTGLVAK"
BASE COUNT         5914 a         5533 c         5822 g         6101 t
ORIGIN      
        1 gggtaacaag atcatcgatc atcaccgcca tgtaggcctc ggaacgatcc aatagaaaag
       61 caggggctcc ctgaacggcg caggcagcgt tgattcccgc ccagagaccc tgtgccgcgg
      121 cctcttcata acccgatgtg ccgttgatct gaccggccag atacaggcca tcaactagtt
      181 ttgtttccag cgtgggtttg agctgtgtcg gctgaacaaa atcgtattcg atggcgtaag
      241 ccgggcgcat gatttgcgcc tgttccagtc ctttgacgct gcgcacaatc ttatactgca
      301 attccagtgg cagacagttg cccagacctt tggcgtaaac ttcctgcgtg tcgagtcctt
      361 catgttccaa caccaccgga tgactctggc gttcggcaaa gcgcatgacc ttgtcttcca
      421 gtgaggggca gtagcgggcg ctcacgcctt tgatgatgcc ggagtataaa ggagaatgct
      481 gaatgttttc gcgtatcacg cgatgcgttt cagcggatgt ggcggaaaaa tacgacggta
      541 gccgctcggt gcgcagtttt tcggttgtga aggaaaaggg ccggggatcg ggatcgctgt
      601 cctgccgttc caagcgggaa aaatcaatgg aagaggcgcg aagcctgggc ggcgttcccg
      661 ttttcatgcg gccgatatcg aagccaagct ctttaagatg gttggccaat ccgatggacg
      721 ccagttcacc ggcgcgtccc gatggagttt gcgaagggcc gacgtgcacc agcccgttta
      781 agaaggtgcc ggttgtaata ataatttttt tagctccgta aaaatgctcg ctctgatcca
      841 gcacgcctat gacttttccg tcttcaatca ccagtttttc aatcatggcc tgccggacat
      901 aaaggttagg ttggttttcg accacggcct tcatcgtcag acggtagagt tgcttgtcgc
      961 actgcatgcg ggaggaatgc accgccggac ctttggaggc attgagcagg cgaaaatgca
     1021 cggcggtttt atcggtgatc ttgcccatct cgccgccgag ggcgtcaatt tccttgacca
     1081 gttgaccttt ggccagtccc ccgatggccg gattgcagga catcaacgcg atggaatcca
     1141 gattgatgtt gaacagaatc gtccggcaac ccatgcgcgc gcaggccagc gccgcttcgc
     1201 agccggcatg tccgccgccg acaatcgcaa catcataatt gtctgttaag ttcgtcattc
     1261 tttcaatcaa acacttgctc ttgttttaaa tcaatagtaa acacgaacag gtcatgccgt
     1321 tgcaaaacgg tatccagtat tttcattaag ttctggatac cggccttcgc cggtatgacg
     1381 ccattgatgg tactttctat tcatgacaag ttttttacgg catagaatgt aaaaaaacaa
     1441 cataaaaaag aggttgcttt gcccaaaccg gatttttgga agaataacaa aaactactac
     1501 gacctgaaaa gttactggcg caatctgttc ggctgcagcg tgcacaagct gcaaattgac
     1561 gcaggattta cctgtccgaa ccgtgacggt catgtggcca tgggcggctg tatctattgt
     1621 gacggcagag gttcaaagtt gcgtcaggct ggtgagttgc cgtcggttac cgagcagatt
     1681 gcgagcggaa aaaaatatta tacaaaacag gccggaaagt tcatcgctta ttttcagaca
     1741 tttacgaata cttatgctcc ggtggagaaa ctgcgcgcgc tctatgatga agcgctcaat
     1801 caggaagacg ttatcgggct ttccatcggc accaggccgg attgtatcgg ccctcatgtt
     1861 gtggagttgc taagcggata cgcgcaaaag tatcatgtct gggtcgagct ggggctgcaa
     1921 tctgttcatg ataaaacatt aacgttgatc aaccgcggtc acagttttca gcaatttgag
     1981 gatgctgtaa atgctctggc cggacacggc ctgaatattt gtgtgcacat catcatcggt
     2041 ctgccgggcg aaagcgacga agatgtgctg gccacggcca aaacgcttgc agccatgccg
     2101 attcatggca tcaaaatcca ttccctgctg gccctggacg gaacggctct tggtgaaatg
     2161 tacaagaaag gacgcatcaa catgatcacc aaagaaaaat atgtttctct ggtggccgat
     2221 gttctggagg tgctgccgcc gcaaaccgtg attcagcgcc tcaccgcgga tggttaccgc
     2281 gatattttcc ttgcacccga ctgggcaatg aacaaactgg acgtgttaaa ctcaatcgat
     2341 aaagaactga tacggcgtga ttcttatcag gggaaaaagt accggacaaa tcgcgctgaa
     2401 tcacttgatt gatatttcct tagttcgaac cgcttcaacc cttcaataag cgctatggct
     2461 tatctttttt tgaccgtttg gaattcagat aaaatccata accgataaaa atcaaaataa
     2521 atactaacga cggggggaag aaggcgacaa tcaagatgca gacgaaagcg atgacaagca
     2581 ttcctgaata cgggtgatct tccaatgcat ccaacatcgg attaaacaat cgatctatca
     2641 ttcgctcttt ttcttccatt gttccgaaat ccttttgttt atgcatggtt gtttgtgcaa
     2701 tgtcttattg acatcatagc ataattcttg atcttttaaa gaggcaaata ttccaaaacg
     2761 acaggaagga gtgtccgttg gtgaggcttg aaatgaaaat aaaagtccat gccgctaatt
     2821 tatcaaacgg aacaaactga tgatcaatga aaatatcagt aaaattaagt atagaatggt
     2881 aagtatctcg gcggccctga agtctttgat tttcttcgcc attaaatcat ttaactttcc
     2941 gtccttgtag atccaccaga gaaatgtcac agaaaaaatt attataataa tccacaaaat
     3001 tgcattgttc atttcattat tcttccatgt acggttttac atgagcatcc cattcggctt
     3061 cagatttgca cgtcttttct aacacattac acgtaatcgt tttgccgttt ttttgggaac
     3121 ggaccgcccc gtatatttta tttcccttca tctctctcaa aacctttaag ggtttttttg
     3181 tatagtttaa taaaatgaaa catttatcca gtctcgtgtt gtaatggctc tgatgggtaa
     3241 acggatctct gaaaaattta ttttcgtcaa ccctgaccca cgcgtcacaa tctttttcgc
     3301 attgcatctt taatgcatgg agttgctcct gtcgctcgga cgttacgcaa atgtcatggt
     3361 atatttcttc cggcttgctt ccggggatga tgcccgtcca ttcgctatta ttatatgttt
     3421 cttcatataa gacgctttca ttgtcgtcat aatttaccag cttttctatt ttactctgtt
     3481 tgtttctgca atccattttt aacaaaatcg tctggtgatc gagtttctgg tatttgattg
     3541 atttcttggg atccgattct ctgaaatgtt caatcaaata ttttctttca tcgtcggcaa
     3601 tggttctgta agtccataca gatatgctgt cggccgattg aattaaattt attttattat
     3661 agtaaaaatc atcgcttaaa tgtttccaga ctttcgggtc gggctgctcc gaaaaacaaa
     3721 cgggaggtga tgacacaatg gacgctgcga tgaaaacaac gagagatgat aaaaacattt
     3781 ttttcatcat aggaatctcc gaacaattca tagccgactt atttcataac aagttgaaag
     3841 tatcataaca ctgttcctta atttaaaaaa agagaaatat caaccgcgtt aactggatct
     3901 ggaaatcttg cacggtggct gataggaacg attttggaat atcgggtgat aaaaaacaag
     3961 agagaaagga tgaatgaccg gaaaacagcg cggcagcggt attatgggtc ttctgtatac
     4021 atggcttcga tgatatcttt gtattgcgca gagatggatt ttcgttttac tttcatggtc
     4081 ggagtaactt caccgtcttc cgtgtagagt ttcttgtcga gcaaatggaa tttgcggatg
     4141 ttttccacgc gcgcgatttg cctgttggcc ttttccacct cgtcggaaat aagtctgatc
     4201 acttcctccg cacgggttag gctcgcaaac gttgtgtatt ggatcttatg gtcctgtgcg
     4261 tatttgacga cgttttcttc atcaatgaca attaaagcgg tgatgtactt gcgccgatcg
     4321 ccgataacca ccgcgtctgt aatatagggc gagaatttca acagattttc gatatactgc
     4381 ggcgcgatat ttttgccgcc tgcggtgata atcaaatctt tcttacgatc ggtgattttg
     4441 agataacctt ccgcatcgat ttcgccgacg tcgccggtat ggaaccatcc gccttccagc
     4501 gcttccgccg tggcttccgg ttccttgaag tatccctgaa aaattccacc gtgtttgatc
     4561 aggatttcac cgtcttcggc aattttcact tcggaatccg gcagtgcgcg acccacggtt
     4621 cccactttga aatgttcctc gtcggacatg tgcgtaatgc cggtcgtttc cgtcagcccg
     4681 taaccttcac ggatcggaat gccgatgctc tggaagaatt tcagtacctc atgggagatg
     4741 ggggccgctc cggaaaatcc cagccgcacc ttgtcaaatc ccaggcgttg tttaagtttc
     4801 cagaaaacgc tgaaatacgc caattgataa ccaaggaata aataccaggg gatcggtttc
     4861 ccggccagtt tgaaaccgtt gtatgttgtt ccgatcttaa aggccagatt aaaaacagtg
     4921 cgttttaacc acgtggcatc ggccattttc aggacaatac cggaatgata cttttcccag
     4981 atgcgcggga ccgcatgaaa gagggtcgga gaaacatcgc gcaggtcgtt catgaccgtg
     5041 tcggtgtttt cggtaaaatt gacgatatgc cccgttgtga gatgcatcat caagtcgaat
     5101 atctgttcat agacgtgatt gagcggcaga aaggaaatgg tttcatcatc gacgctcagt
     5161 ttggtaattt tctggctttg acgcgcaatc cataaataat tttgatggga gatttgcgcg
     5221 cccttgggcg gaccggttgt ccccgatgta taaatgatgg aagcggtatc ttcgctctct
     5281 cgtccggcaa caatcttttc atacagaccc ggtctttcct tatcgatctt ttccccaagc
     5341 tccagaagct tttcaaaact catcagcatg ggatcgtcaa aatgcttgag gccttccata
     5401 tcccagacga ttattttttt caggcgcggt gttctttcgc gaaatgccag agccttatcg
     5461 aattgttctt catcttcgca gaaaaaaacc accgattcgg aatgcccgac aacatattcg
     5521 cattccgccg taggattggt cgtgtacacg ccgacaaaga taccgcccag cgacatgacc
     5581 cccatggccg aaaaaagcca ctcgggttta ttctcgccga caatggccac gtgatcgaag
     5641 ggcttgacgc ccagcgcgta caatcccacc gccactttct tcacgttttc ataatactgt
     5701 ctccagctaa tgtcgtgcca gatgccgtaa tccttcatgc gcatggcaac cctgtccggc
     5761 tgtttgtcga ccagccattt cagcccctga ggaagagttt tgatgtcgtt ttcagtcata
     5821 tgtatacctc agcggaaacg cactttgcgg cgataacgtt tgaagccttt gaccgatgtt
     5881 tccgtggcaa tgcccagata aaattctttg acgtcgtcgt cttctcttaa aatttccggt
     5941 ctgtccgcac ggacaatgcg cccgttttcc atgagaaaag cgtaatgcga atatttgagt
     6001 gccatgttta cgttttgttc cacaagaaag atcgttacgc ccttctgatt gatgttttga
     6061 atgatactga aaatctcttt ggtcaataag ggcgaaagtc ccagcgacgg ttcgtccaac
     6121 atgagcagtt tcgggcggct catcagcgcc cgtccgatag ccagcatctg ctgttcgccg
     6181 ccgctcaaat gtccggcctg ctgatttttg cgctcgctta atatgggaaa ctgtttgaga
     6241 atcccgtcaa tgtcatcctg gatgcctttt ctgtctttgc gcgtataggc gcccatgaga
     6301 atgttttcca tcacagtgag ctcgggaaag acctcgcgtc cttccggcac atagctgata
     6361 cccagccgga caatttcctc cgtgtccatt cggtcgatcc ttttacccag aaattcaata
     6421 gatccttttt ccggctgatc gtcatccaaa aggtgcatga tggttttgag ggtggtggtt
     6481 ttgcccgctc cgttactgcc gagaagcgcc acaatggagc cttctttgat ttcgaacgaa
     6541 atgccgtgca gtgcccgaat caagtcgtac caggtttcga tatttttaac cgagagcatt
     6601 attcctcctc tcccagatac gcggcaatga cttcggggtt cttttgcacg tcctgaggcg
     6661 ttccctcgat gattttcacg ccctggttga tcgccagaat cctgtcggaa atgcccatca
     6721 ccatattcat gtcatgctcg atcagcagaa tggtgacttt gtaatcttcc tgaatatcct
     6781 tgatccagat gcgcagatcg tctttttctt ccgtgttcat gccggcggac ggttcgtcca
     6841 gcagcagaac tttcggttcc aacgccagcg ctcgtccgag ttccacgagt ttgcgtttgc
     6901 cgtagggaag gctggaaaca aaaagatctc gcagggactg gagatcaaga aagtcgataa
     6961 tgtgttccac gatttcccgg tgtttgagct cttctttcgc ggcgaaagaa cgtttccccc
     7021 acatgaaagc gccactcgtg attcctgtct tcatatggat gtggcgtccc aacatcaggt
     7081 tatccatgac agtggcgtta ccgaaaagct gtatgttctg gaacgtgcgc gccacacctt
     7141 tgcgggcaat ggtgtccgga cgcaggccga caaggttttc attatccaga aaaacttccc
     7201 cggcatgagg tttgtaaatg ccgctgatga tattgaaaat cgttgttttt ccgcttccgt
     7261 tgggaccgat gatcgaaaat atcttattgt actcaacctc aaagctgaca tcgctgacag
     7321 cccggatgcc gccgaaggag atgttcaggt tttccacttt aaaatgcgcc atacactgcg
     7381 agcctgctga tcgttattgg caacgaaaat atttggtggg aggcagggca tcccccgcct
     7441 ccggcgatat tggtatgtta ctttttcttc cacgttgcca gttcgttaga tgtccagtcc
     7501 tgaagaataa tatagctgga attcggaccg catttcagaa tctgaatgga atcgcaaccc
     7561 tgatgcactg tcgggctcca gtttacaacg gcgcccaatc cctggaaatt ctttgtttta
     7621 ttgagttctc gcagcagggc ttccgtgctc agtttcgggc cgacccgctt gagcgcatcc
     7681 accaacggtt ccgcaaacat aatgccggcg gtgtagaaaa cgccgaaacg ctcttcaggt
     7741 gcgtatttct tggcagccgc gcgatattta ttcatcagcg gatgatccac atcggtcggc
     7801 aggccgattc cgccggtgat gacgccttcc cacaacccac cggtaatctt gttcatcagc
     7861 ggaaaatcca tcaacgtgtc ggaagaaatc cattgcggtt taaatccgat ggttgcggca
     7921 gttttcagcg cgattgccgc agaggtggga ttgacccaca gaaaagtcac ttccgcgccg
     7981 gaatttttca gtctcaacat ctgcgaggaa agatcttttt cgccgggttc aacggatatc
     8041 tcttcgacaa gtttcatctt atacgtattc aggcgagcct tgcagccctt gaggccgctc
     8101 ttgccatatg cgtcgttctg atacaggaat ccgatttttt tggctttcag tttttccacc
     8161 gtatatttgg tcagaatgct ggcctcatca tcatagagag tatacatggc aaagaggtac
     8221 ggattgacgg gaaacacata ctcgtcaatg gctgtcgacg gattgaccca gatgatttta
     8281 ttctgcgcca gataatcttt cacggccatt ccgcaggcag caccgacgcc gcccacaaaa
     8341 gcaaaaattc cctcacgctc gacaagttcc ttgaccacgg ctttagtctg ggcggggttg
     8401 tactgatcat cgcggatgaa atatttgatt ttacgtccgt ggatgccgcc ttcatcgttg
     8461 acaatttgaa acagaaggcc gctccctctg gcgaccgatc cccagggtgc ggcaggtccc
     8521 gtctgcggcc cccactgggc gatacggatt tccttgttgt caaagcccga tttggcctgg
     8581 gcaaaaacaa gaagcgaagc aataaaaaca gcgatgacaa tcgtcaatgc aatgatgaat
     8641 cttttcatag tgacctccat gctttaatga gtttagataa atgattaacc tctttatgcc
     8701 gttatggtct gggggatcaa cccttgtacc atttcccttt ttctttcagg aacgtgaagc
     8761 tgtaattaaa gatactgttc ggacctgcgt gtccaaacgt cacttccacc tccacctgaa
     8821 ccaatttgtc cacagcggga taattgtgcc ggttgtggtt atcccgcaga tggtaaattc
     8881 tgacaatttt acaggtgttc aatgccgcgg aagaattttc ggcgatgtct ttcaaatatt
     8941 cttcgtacgt gtgtgtcttt ttataatccg atgaaggcgc caaaagcgca tagacctgcc
     9001 gcgaatcttt tttcatttcg gcagtcagat agtttcgggc ggtttcttca atgacgcttt
     9061 tttccagaaa caattcgagg cggtttcggg gcgcatccca gataaccaat ccgaaaacaa
     9121 ccagcaccgc gcaagccagg ataaggattt tcacggtttt actcatgata atgctccgat
     9181 gcttcacgtt acactttctt gatataatgt ttcgcgaaca gtccgctcgg cctgacggta
     9241 atgatcagga tgataacaac aaacgcgacg atcggtttcc attccggcca ggcgtaaccg
     9301 aagaaatttt cgataatacc caaaatcagg ccgccgacga cgacaccggg aatgctggtc
     9361 agaccgccca ggacggccgc ggcaaaagcg cgcataaacg gttccatcat aaaattggga
     9421 tccagcacgg aacgcgccgc gaagaacatg gccgcgacgg caccgatcag cgaactcatc
     9481 gcccaggtaa tggagaatac tctttccact ttgatgccca tgatcttggc tgcgttgacg
     9541 ttttgctggg tggcgcgcat ggcgatgccc agcttcgaat aacggaaaaa taagaacagg
     9601 gatatcatga tgatgatgga cagggaaaag acaccgatcg tccattcgtt aataaagatg
     9661 ccgggcagca tttcatgtgt cttggtaacg gacagcagaa agggaaaatc tttctgttcc
     9721 gctccccact tccagccggc cagtcccata agcagcattt ccgcgccgag cgtaataatc
     9781 agaagcccca gcacggtcgg atctttggcc ggacgcagaa aacaaaactc gattgccatg
     9841 cccagcagga tggcaaaaat cagcgtaacg ggaaaggcga gccagaaggg cacgttatag
     9901 accgtcaaga catgataggc gacaaaggtt gcaaccattc ccatttcacc ctgggcgaag
     9961 ttgatgacgt ccgatgtttt gtaaatgatg gcgacggcga cgccgaaaag accatagcag
    10021 gccccgatgg cgattccctc gatcagcagc atttcaaaca tgacggactc ccttcagctt
    10081 ttttaaaaag gccaggtctt ccagtatttt ttgattcgga tccagatgcc gaacatgccc
    10141 agcggttcaa aaatgacgat cgtgatcatg atgagtccca gtgcaatgct ggagaaattg
    10201 gccagcccga tcaccgtaaa ccattttttt gataccgtcg tgagaagtgc ccccagatag
    10261 ggcagatttt ccacattttt caataagtcg tactggagat aggtgataag catggcaccc
    10321 atgatagcgc ccataatcga tcccaggccg ccgacggtaa tcatgaccag aaagatgatg
    10381 gaaagaatca ggttgaacgt aaagggatcg aaaaagccga caacaaaacc gtaaaggccg
    10441 cccgccacgc ctgcgtaaaa agcgcttacg gcaaatgcca gggttttata aatcgtcaga
    10501 ttgacgccca tgacttctgc tgcaatgtcg tcgtcccgga tggacataaa cgctctgccc
    10561 accctggtct tcatcagatt gcgcgcgccg atgaccagca aaacagtcat catcaatatc
    10621 agatagtaaa tctttacatc accctggatg acgtaactga acgatgtccc tgaaataccc
    10681 atatcaagcg gcggggcggt gattcccatc ctgccgccaa agaaatccca ccggccgatg
    10741 atgtgcataa tggtcaaccc gaagcccaga gtggcaatcg caagataggg gccttcaagc
    10801 ctcaacgccg gaaggccgat tataaagccg aaaaaggctg caatcaacgc ggccagaatg
    10861 attgcagcga agaacggaaa gtggaaattc atcatgatta atgatgtgcc ataggcgccg
    10921 atggcaaaaa aaccggcatg tcccagcgat atctggccgg tgtagccgac aaggatgtta
    10981 aggccgaccg ccagaatgat gttaaccatg ataatattta agaggtagat gttataagac
    11041 ggtaaataaa cgggaagggt aaaaagaaaa accagtaata cgatggacca cgtcagaatc
    11101 gtgccgcttc ggaataactc aacgtcctca tagtaatcgc gtttcatgtc catggcataa
    11161 ccacatatcg atggttggtt gacatgttgc tgtacgttga tcttttttgc gtctggatgt
    11221 tctaaaaaca tagcaagacg tccgaaatgt gtcaagataa tttgaccagg aacaaaattg
    11281 tatcagtgtg ggattaatgt ataaaatgat aaaaattaac tgtaaattga acagaaaaat
    11341 tgctaaaggc atattccaga aatttggtaa ggaatgaaca atgatcgcga ttatcgacta
    11401 taacgccgga aatattacca gcgttgcacg cgctctgcag agtatcgggc aggaattcat
    11461 catcacggat gatgcgaaaa agctcgatgc ggcgtcgcgg gtgattttcc ccggtgtcgg
    11521 cgcggcgggc gaagccatgg cttatctgcg ccaaaagaaa ctggatgtct ggctgcgaga
    11581 ttacatcaaa accggcaagc ctctgctggg catttgtctg gggacgcaga ttattctgga
    11641 ttactccgag gaaaacgaca ccccgtgcgt gagtcttgtt gccggttcga ccaggcgttt
    11701 cccggaaaca ttagcgttca atggtgagtt attaaaaatt cctcatatgg gctggaatag
    11761 cgtcaggctg ctgcgcagcc atccggtgtt tgatcatgtt tcgccggagg ctgaatttta
    11821 ttttgtgcat tcctattatc cggcgcctgc cgatgaagcg gcgattttag gcgtcaccga
    11881 atacggcata acgttttgtt cggtgcttgc caggaacaat cttgtggcca tgcagtttca
    11941 cccggaaaaa agcggtcgcc cgggccttca gattttaaag aatttctgca catggaggcc
    12001 gtcatgatca gcaagagaat cattccctgc ctcgacgtgc gcgacggcaa gctcaccaag
    12061 gggatcaagt ttaaaggcaa cgtagatatc ggcgatcccg tggaagcggc gcgggaatat
    12121 tacgcacagg gcgcggatga aattgtattt tacgatatta cggcgtcgtc ggacaaacgc
    12181 aacatcatga tcgatgtggt gcggcgggtg gcggaaacga ttttcattcc tttctctgta
    12241 ggaggcggaa tccgcaatct ggatgatatg cggaacgtga tccatgcggg agcggaaaaa
    12301 gtgagcgtga attccgccgc ggtggaaaat ccgcaaatca ttacgcaggg ggcaaaagct
    12361 ttcggcagcc agtgtgttgt gctgggcatg gatgtgaaac aggtggacgt ttccgatgcc
    12421 attccatcgg ggtatgaaat cgttgttcac ggcggccgga tttataccgg aattgacgcg
    12481 ctgtggtggg caacggaagc ggagaaactc ggcgccgggg aaatctgtct gaattccatc
    12541 gacgccgacg gggtgcaaac gggctatgaa ttgaatttaa cccggctgat ctccgaaaac
    12601 gtgcatattc cggttatcgc gtccggaggc gcgggaaaac ccgaacatct ggcagacgtg
    12661 ctgaccaaag ggaaagctga tgcggcgctg attgcttcca tggtgcatta ccgcacctat
    12721 accatcgctg aaatcaaaca ctatttgagc gcaaagcaaa tcaagacaag attgttctgg
    12781 taatttttgc tttttttcac taggaaacag tattgtgaag tattataata gtaatagttt
    12841 tacgctagga ggcgatataa agtggataaa aagaggtatg aattaaatgt ccagttggcg
    12901 caaatgctca aaggcggcgt cattatggat gtaaccaatg ttgagcaggc aaaaatcgcg
    12961 gaagaagcgg gagcggtagc cgtcatggct ctcgagcgtg tgcctgccga tatccgcaaa
    13021 gacggcggcg tggccaggat gtcggatcct gccatgattg cggcgatcaa aagggccgta
    13081 tccattccgg tgatggccaa ggcccgtatc ggccattttg tcgaagcgca gattctggaa
    13141 gcgctgcgta ttgattatat cgatgaaagc gaagtgctga ccccggcgga tgaagaatgc
    13201 catattgaca agacaaaatt ttcgataccc tttgtttgcg gcgcgcgtaa tctgggtgaa
    13261 gcgttgcgcc ggatcgctga aggcgcggcc atgatccgta cgaaaggcga ggcggggaca
    13321 ggcaacgttg tggaagctgt ccgtcacatg agggccatga accgggaaat cggtcaactg
    13381 gtgcagatgc ccgtggaaga agtgacagga ttctgcaaaa ccaacggtat cccgtacgaa
    13441 cacgcgctca aagtgaagga attgagccgt ttgccggtgg tgaattttgc cgcgggaggc
    13501 attgctactc cggcggatgc cgcgttgatg atgcaactcg gctgcgatgg catttttgtc
    13561 ggctccggta tcttcaaatc cgccgatccg gccagacggg cacgggctat cgtcaaggct
    13621 accgcttatt tcaatgatcc tcaaaaagtt ctggaagcgt ccatggatct gggagaagcc
    13681 atgccgggcc tggaaatttc gcagattccg ccggaacaga ttctcgccgg gcgcggctgg
    13741 taaatcttta tgtcactgcg tttagtgagc gataaacctt ctgttatcgg tgtgcttgat
    13801 ttacagggcg gtgtccatga gcatctggag catctggagc ggttgggtgt cgcctgtaaa
    13861 agagtaaagc agacggaaga ttttaaagac ctggcgggcc tgattattcc cggcggggaa
    13921 agttcctgtc tttcccggtt gctgactatt ttcggcatga aggatgtttt gctggcggcg
    13981 caccgccggg gaatgaaaat ctggggcacc tgcgccggag cgatcttgct ggcccaaaac
    14041 gttgtcggcg aagacgcctg cctcaaactg atcgacatca ccgtcgaacg caatagtttc
    14101 ggttctcaac tggacagttt tgccggcgaa gcgttgattc cgggtatttc tgccgatcct
    14161 gttccgctga cgttcatccg cgcgcccaaa atacttgctg ctcaacccga tgtccgcgta
    14221 cttttgaaaa ttgacgacta catagcagca gcggaaaatg actccgttct ggtgacggtc
    14281 tttcatccgg aattaaccgg ctgtctggcc ctgcatcagt actttgcggt taaatgcggt
    14341 ctgaaaaccg caaccgggca agccggacat tcggatccgg actgggatat ttcaagctgg
    14401 acaaaatatg ccgcccttgc cggcagagcc ggtgactgac gaaaactgtc gtttaaaaaa
    14461 attgcataac aatcccaaaa tgcttggtct gcacattgaa acaaaatggt gttgacaggt
    14521 caaaatatct tttttaaagt gcggacatat taaggagcgt cgtgcagaat attttttccg
    14581 aactgaaaac agattcccgc ttcatccgtt ttgtgatgaa ggtgggcagt ccgtcaccca
    14641 tggatcccga cgaacaacac agacggatac cggcggtgtt tgtgctgatg atcatcagcg
    14701 tcgccacggc ggtttccggc atctatcatt gtagcagcgg aaaaaatccc cttgtggttt
    14761 tggacgtcat cggttttatc ctttccctta tcatgctgct ttatctccgt cagggaaaaa
    14821 atcccggcat cgcgtacggc atgatcagca cgggttttgt tctcttgtgt tgtgcggcga
    14881 ctgttctggg cagaacggag atgtcgctga tcttctgggc cctcgtcctg cccgccggat
    14941 gtttttccat attgggccgg aaaatgggta tgttcgttgc cgcattcttt ttctttatca
    15001 atctcctgct gatgaattta ccggagcata tgatcctatc aaaacccttt ccggcatcag
    15061 tggctgcccg tttcagtgtt gtttacctga tcctgacgtt tatgctttat tattacgagt
    15121 catcgcatca gatgctgatt cgctacatcc ggcatgagga gaataagttt caaaatgctt
    15181 cccgatacga tccgctaacc gggctttcca accgcaggga tattcttgaa aaaatggaaa
    15241 atgagcggga gcgtcattcg cgtctggaaa aaacttttac gctcattatg ggtgatatag
    15301 ataattttaa aatgctcaac gatacgttag ggcacgaagc cggtgactat gtactgcaaa
    15361 cgatcgggcg catcctgaag aatcaggttc gtggaatcga ttgttcttcg cggtggggag
    15421 acgatgagtt tctgattctg ctggtggata ccgatctgga aggcggacag cgcgttgccg
    15481 aaagaatcag aaaaaaggtt gagaatacca gctttcagta tcagggcaag ggacttgccg
    15541 tgacgatgac attcgggttg agtatttatc agagcgccga cgaacagatc gacgcatgcg
    15601 tcaggcgttc cgatgacgcc cttgaggaag gcaagcggca ggggaaaaac agagtggttc
    15661 ccgtttagtg tgtttcatat ctgtcaagga agaggttcaa attgttttct tcgtcgactc
    15721 caagccggtt ataaatgaaa gattttcaga aaaatggtcg ccagtcccag gaaagtcaaa
    15781 aaaccgaagg cgtctgtaaa agctgtcagg aaaatgtgcg atcccagggc cggatccagc
    15841 ttgaggcgct ttaacgccag ggggattaac gcgcccacaa gggtccctgt gaaaatactg
    15901 ataatcatgg ccagaccgag aatcagtccc agaatgggta tgcctttcca gaaatatgca
    15961 atgatgccta tgacaattcc tacggcgatg ccgttggcaa tgccgacggc aatttgtctg
    16021 aacaaggcgc gcttcgcgtt ctcaaaggta atttctccaa gcgccatccc gcgtacgata
    16081 agcgtgaggg tctgactgcc cgcgttgccg cccaatccgg ctacaaccgg cataaagaac
    16141 accagcgcca ccatcaattt gatcgtttct tcaaaaaatc cgatgaccag cacggaggca
    16201 agagccgtta taagattcaa ataaagccag gggagtcttt tttttactga ttcggtggtg
    16261 ttattgaaaa cggtgtcgtc ttcacccaat ccggcgatgc ggtaaatatc ttccgatgtt
    16321 tcttcctgga tggcatcgac aacgtcgtca atggtaatcc gacccagaag gcgctgttga
    16381 ttatccacaa caccgataga gaccagatcg tattttttga aaagacgggc cacttcttcc
    16441 tgatccatgt cgtggagaac actgggaata tcctcgtcga taatatccag aatcggcgta
    16501 aagcgtttcg ttgtaataat actttgcaga ggaacggttc cgatcaattt atcggtttca
    16561 tcaacgacaa aaatgttatg aatattttcg atttcatctt tggcgtcggc aacggcctga
    16621 aaggcatcgt tgatggtcgc gtcttttctt acggaaacca gttccgactg catgataccg
    16681 ccggcggtat cgtcggcgta tttgagcagt tcctgaactt cctgggactc ttccggttca
    16741 atcgcttcca gaacggcctt ggcctggtca tcggataact ccgcgatgat atccgccttg
    16801 tcgtccgagt ccatctcttc gataatgtcg gtgagttttt cgttggaaag atcttcgata
    16861 agctgctcac gggatgtatc ggataattcc agaataacgt cggacgcttt gtccacatcg
    16921 agcagcgaga aaagatgtaa gcggtcttct tcttccagtt cgtcgatcaa atcagcgatg
    16981 tcggccggat gcatatcgga gaaaagatcg atgatgtcga attcccggtt gcggctgatc
    17041 agatctttta atgtctccag cgcggtcggt tcttttttct tgaccttttg cataggatcg
    17101 cctgatgttg aagtcggttt aaaaatgatt ttgttgattc aacaatgaac tagagttctt
    17161 gctcatgcgt atttacggga aagaagattc ttttctgaaa tattcgcacg tgaagattcg
    17221 ttttccggca gcgagactat aaggaaattc aatacatatg tcaatgtttt tctgaaaaaa
    17281 agtcataaaa aacaattttt gtcataaggt tcatccgagc cgacggttgt tttctgtatt
    17341 tgaaaatcct gcatccgggt gagatttttt tctcgttgac aggggaaatg acttgcttat
    17401 attctctgcg ggagttttat tttcttggcg caaaggggac gatacttatt aaagcggaca
    17461 acattgaaaa aaatttcttt tctgtcggtc gctactgggg aaaccttaat gcatcgttga
    17521 agagaaaaga atccgtctgg tggatgaata ccggtgtggc tgtttccgat atgaattggg
    17581 catggaatga aaccccgctg gcggccccgg atacggcaat cgtttccgaa atcagggaag
    17641 attacaaaaa aaataatttg cgtttctggt ggtgggtcta tccatgcgga caatccgcgg
    17701 ccacatccgc aatactgcag aacgcaggat ttcgtttata tgccaatgtc tgctgtatgg
    17761 cggctgattt aaacgaatca tttccggagc cgtacgcgac cggtgacatg gaaatatatc
    17821 atgtccgcag ccgggaggat ctggccgtgt gggaggacgt tgcttttcat ggttttgaga
    17881 tgccgcggca cgcgagagac ccattcgggg cctttgtttc ttcttttgaa acaggagatc
    17941 agtttccaca gaggtttatt ctgggttgta ttcaagggaa acctgtggcg acgtccctgc
    18001 tttttaagga tcaacgcacg gcggggatat actatgtatc aacgctcccc tcttacagaa
    18061 acaggggatg cggcttgagc gtcacgcagg ccgcgatgca attggcgcgg gaaagcggat
    18121 ttaagcatgt tgtcttacag gcgacgccca tggggaaaag agtttatgaa cgattgaatt
    18181 tcaaacgata ctatcatgcc ggaatctata ccttatagaa gatcatgacg cttttatcct
    18241 cttgacaggc atcatcgcgt tacgtatagt ccgccagaca aaaccccata agctattcaa
    18301 aattgcaacg tgctgtcggc aacccatgat aatatctttc tgaaaaggac agttcatgaa
    18361 aagaaactcc ggtcataata tagcatgcga caatgagtgc ggtggccttc atcaatccaa
    18421 ccccttgtcc cttgccaaaa atgaaatatc ctcgacgctt tcctggttta tgacggataa
    18481 tgcgctatat ccgggtgtgg ataaacactc ttccccgctg cgttatgaga actggtcggg
    18541 acgatatagc gaatatattt ccagacaaga agccgaatcc atcgtgagcc gctttatctg
    18601 ccctgagaaa gatgaaaatt ttacactgtt tttcgtcagt gattataagg atacgtacct
    18661 ggggaaaaat tttttaaagg acggcagcga tatttatctg aaacattacc ggatgaccga
    18721 tgcgccccgg gaaatccgtc tgagaaatgt tttgcgccgg tgcctcgcca gaaaaaactt
    18781 caatgctttt tatcagctgc atcaaaaaaa catcgaagcc gttaagcctt tgttctatgt
    18841 tatgaagagg ggccgctgga ttcccgatga ggtcatcgct gtcagcaagg gggcgtccgg
    18901 caacaaaaca attgagaccc tcatggcgga cagccgggac aatgatgaat tcaggcttct
    18961 ggccgtcaat ctcggctcgt atgtcgccga acttcaatcc agggaaattc tttttaaaga
    19021 attgtaccgg aatctgatcg cggtcaacca gacgacgcat tgggatttta ttctctgcga
    19081 tctggatgaa atcaggtcgg tctcggaaaa aagattttac aagcacagga agcacgttga
    19141 aaaattacgc aagaaaataa agcgctacgg cgattctttt ctccatgctt ttgatcaggg
    19201 gtatcagaca cggatgcatg atcagacggc cccgtcgaga cggtgattta tcggggatgt
    19261 ttgccgaagg gatgacgata aaaaataccg gcaatgggtt gtcggttaaa ttcttccata
    19321 cagatcttca aaacgttcta tatcgccatc atcacagcgt gctcccgttt ggacctcaat
    19381 aatgaccaga tgaccgtctg catggttcag gacgcggtgt ttaacctttg ccgggatatc
    19441 caccgatccg ccggcgctca gcgatatgat cttgtctcca acggttacgt ctccggtgcc
    19501 ggcaacaaca aaccagtgtt cggaacgaaa atgatgaagc tgtagactca gaccccctcc
    19561 cggttctaca ataatgcgtt tgactttatg gccggcggtc tccgataaga cttcaaaact
    19621 gccccagggg cattgggtca tttttcccat tttcatcacc tgtaagattt gccgcatatc
    19681 taacacaaat tttgaacggg gaaaagcgct ctttttcatt ttccatactc gctttcgaat
    19741 ttaaattatg atacttacac ataccgcgca tcgatcatgt cggcggaaag taacggggtt
    19801 tgatgaacat ttcctacacg gtcaggcgaa gcagaaaaag acaaaaaacc atgtcgcttc
    19861 aggtttgcga tgacgcgaaa atcgttgtat ccgctccttc ttttacaccc gccgatgaca
    19921 tcagacgttt tgtggaggaa aaacaaggct gggtggctaa aatactggcg cgacagaagc
    19981 aggaggccgc tcacgccaaa gagaaaaaat atgaaaccgg cgagcaattt ttttatctcg
    20041 gacagctcta tccgctggaa gtcttctttg aaccttttga gaaggaaggg attgctttcc
    20101 ggaatcatcg tttttatctt aattgccgca gcgataaagt gttaagaaaa caatgttttg
    20161 ttgcatggta ttcaaagaaa gcggacgaga tgattcgtcg tcgtgttgat tttttcagcg
    20221 ggatgcttaa actgccttat gaaagaatca gaatcacatc cgccggaagc cgttgggggt
    20281 cctgttcgga agataacagc ctttctttca gttatcgcct gatcatggcg ccgccggatg
    20341 tcgtggatta cgtgattgtc catgaattgg tgcacatccg ggaaaaaaat cattcgtcga
    20401 cattctggca aaaggtagag gcggttatgc cgtcgttcag agaacagcgt cgctggctcc
    20461 aagataacca tagcaacctg gcactttgag cggacgaagg aaaggaatga taacgtatcg
    20521 acatggaaca cattgaaatc attgatttga aacaccggga tggcctggcg gagcgtttca
    20581 aaaaaatgaa tctgcggatt tccgattata catttgccaa tgtctatctt tttcgcaaca
    20641 ttagtcatta cgagttcgtg accaaagaat gcggtttgtt cgtttccggc tggaataagc
    20701 agaatcaaaa ttatgtcatg ccgttgaatg atccgcgcgg gtgtgacgtc aaaacatttg
    20761 ctgaaattct tggagcaaga aattttttct ttcccattcc cgaggagtgg cttgtttatt
    20821 ttccaaaaga caaattcagc gtcagttatg acgaaagcga atccgactat ctgtacctta
    20881 ccgaaaacat ggccgggttt aagggaaaac aatacttgcg ccaccgcaac catttaaaac
    20941 aatttctggc atcgtatatt cccgatggcg tgaccctgtc cagccataac tgcaacgatg
    21001 cgatgattgt tcttcaccaa tggcaggagg attcccgcac ggcatccgac aaaacggact
    21061 tcgtccaatg cagcgaagcc ctgcaaaatt tttccggcct tgcgctctgg ggaataatat
    21121 actacattgc cggcaaaccg gccggtttta tcatcggcga agcgctggcg ggggatacgt
    21181 tctgtctgca tttcgccaaa gcatcgaaac ggtaccacgg catttacgaa tatatgttta
    21241 atgatacggc caaaagactg gagtctgcat acaagtatct caatctggaa gaggatatgg
    21301 gtaataagaa tttgcgccgg accaaaagtt cctacggtcc ggagtttttg ctgaagaaat
    21361 accgtgtcgg aattatctaa ttggaaaaaa agacagcgat gagcgaatcg gttaatataa
    21421 catcactgca ggaaggcgat cagggagtga ttcatgccat cgaaggcggg tcggccatta
    21481 ccagccgctt tgccggcatg gggatcgtct ccaacgccag gttccgcatt gcgcagatca
    21541 gcggcggcct gattgtgctt caggttgcca atacccgtat cgcgctggga cggggtgaag
    21601 cttcaaaaat cacagtttcc aaggtcgctc cggaggccga tgtatgtatg ccgcccatcg
    21661 aaaaggaaat ttccgtggcg cttgtcggcc aacccaatgt cggcaagtca accgtattca
    21721 atattctgac cggtttatcc cagcatgtcg gcaactggcc gggaaaaacg gttgaaaaaa
    21781 aagaagggac ccatcgtgcc gacagcgttt tgatcagaat tgttgatttg cccgggactt
    21841 acagtctgac ggcattttcc gaagaagaaa gaattacgcg tgatttcatc atcaaggagc
    21901 aacccgggct ggttgtgctg gtcttgaacg caacggctct ggagcggggt ctttatcttc
    21961 tggccgaagt gctgttgctc aatcggccgg tcatcgtggt cctcaatatg cttgatgttg
    22021 ctgccgatca ggatattgaa atcaacatcc gggcactgga agattctctg ggcgtaccgg
    22081 tgattcccat ggtggccaaa cgcaacagcg gcatcaggga acttgtttct catatctcct
    22141 cgattgcgtc cagtgaatac aaatttcaac cggtaaagcc ggaagtatcc accgatcatc
    22201 agttgatcta tcagcaaatc ctgacgctgg tcagacccta tatccatgat ccttacacac
    22261 ccgaatgggt ggcggtgaaa ttgatggaag gcgacccgga agttacccaa cttgtcgaac
    22321 agcaggttca gaccatcgcg cgcgatcaaa ttgaaaaact gctcatcaag catgaagacg
    22381 ctctgcatgc tgtggtcaac ggccgctacg attggattga agaaatcacc cgttccgtcg
    22441 tttcccgttt taaaatggga caggtcgtgt tgaccgacag aatcgatcat gtcctgaccc
    22501 gtccggtttt cggaattccc atcctgctgg cagtgatggc cctggttttc ttcctgacct
    22561 acagtatggg tgtcccgctt caggaatggc tcagcgaccg tattcagcaa tttatcggat
    22621 ttgccgaacc cctcacgacc gtttggcctt cgtggttgag cggattgctg ctcaatggcg
    22681 ttatcggcgg cgtcggctcg gtgctgacgt ttctgccggt gcttctgata ttttttgcca
    22741 tcatggcttt tctggaggat gtcggctaca tggcgcgcgc tgcgtttgtg atggacaaaa
    22801 tcatgcacct tgtcggattg cacgggaaaa gttttattcc gatgtgtctg ggattcggct
    22861 gcaatgtgcc tgctgttttg ggcgcccgta tcattgagac aaaaaaagcc agaatgatca
    22921 cgctgcttct gattcctttc gttccctgta ccgcgcgact ggccgtgctg acgctggtat
    22981 cagcggccat tttcggtcaa aacggcgcct tcgttgcgtg gagtcttgtg gccctgaaca
    23041 tcgccgtgct gggcattgcc ggcgtttttg tcaataaaac gctctggaag caggatgcgc
    23101 cattcatcat ggagctaccc atttatcaca ccccggatgc caaaaccatt atgatggtgg
    23161 tttggacaag aatactttca tttatccgga aagccggaac ggtgattctt gctgtttcgc
    23221 tgattgtctg gatgctttcg tattttccga atggaaacgt cgaagaaagt ctgctggcct
    23281 ccttcggcag actgcttcag ccattgggca ttccactggg actggactgg aaaatgatta
    23341 ccgctctttt gaccggtctg gtggcgaaag
//
LOCUS       DAONQZ010000035        23078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2487_length_23078_cov_15.560136, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000035 DAONQZ010000000
VERSION     DAONQZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23078
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_23078_cov_15.560136"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..779
                     /locus_tag="PLR13_07115"
     CDS             48..779
                     /locus_tag="PLR13_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HQC19145.1"
                     /translation="MNIDDILKDLTERAGSAVAHYWKTRAGQRDKQRLGGTADQGLRS
                     AVTGGAQMNGFIDLFTEFIVAVGIPEQCVFRKRAVELPGFFRPTKEWDLLVVRENKLL
                     AVIEAKSQVGPSFGNNFNNRTEEAMGSALDLWTAYRERAYLDSPQPFLGYFFMLEDCD
                     ASNRAVKVQEPHFKVFPEFVGASYKLRYELFCRKLVLERHYTATAFIASTQDDGILGH
                     FSTPADDLSLVRFARVLISHLAAFV"
     gene            784..1692
                     /locus_tag="PLR13_07120"
     CDS             784..1692
                     /locus_tag="PLR13_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQC19146.1"
                     /translation="MEETTHRLINGDARELSFLHDESVHLVVTSPPYWSLKRYNEHPD
                     QLGHIQDYEAFLFELEKVWRHVYRILVPGGRLVCVVGDVCVARRNFGRHLVFPLHADI
                     CVICRRIGFDNLNPIIWHKIANASYEVSNGSKFLGKPYEPNAIIKNDMEFILMQRKPG
                     GYRQPTNMQRDASRIDKEEFNRWFQQIWNITGASTKQHPAPFPLELATRLVRMFSFTG
                     DTVFDPFCGSGTTMIAALRTGRNSIGIDIDPEYCRMAARYLKAENADLFATTKLVFEK
                     ATAEPASLVKEDLALYEVRPARKKLA"
     gene            1763..2107
                     /locus_tag="PLR13_07125"
     CDS             1763..2107
                     /locus_tag="PLR13_07125"
                     /inference="COORDINATES: protein motif:HMM:NF022671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HQC19147.1"
                     /translation="MAFSEIELKRIEKEVGDFCEKRSPVEIRDKLSFQYRIKAHDVII
                     YTRRPRWDNPKEWMESDMAKIKYVRTSNMWQLFWQRANGKWFSYEPFPHSKNLKSIIE
                     EIEADQYGCFFG"
     gene            2195..2737
                     /locus_tag="PLR13_07130"
     CDS             2195..2737
                     /locus_tag="PLR13_07130"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQC19148.1"
                     /translation="MKKMQMDKAFTLMESGPVVLITTNDGKKNNIMTISWTMVVDFTP
                     VFAITTGSWNYSFAALQKTKECVIAIPTVDMLDKVVGVGTCSGSDTDKFEKFMLTPVK
                     GKHVKAPLIKECLANIECKVADIVRKHNIIVLDAMAAYIDDKRKEKRALHAVGDGTFI
                     VDGRKMDRRKMMASKLPAGV"
     gene            2805..3434
                     /locus_tag="PLR13_07135"
     CDS             2805..3434
                     /locus_tag="PLR13_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HQC19149.1"
                     /translation="MKKFRIYCSGPLFCAEEIGGMSAIAKVLDDAGFQTFLPHRDGLE
                     SYILRFSNASFPSTISAIRTRVDYAIFSLDAYELIERCSAVVCNLNGRVPDEGMIVEA
                     SLAYAAGKPLVFFKNDLRAPFGGYDNSMLTSLVRGKIVKRLKELPSALEYELSREPEL
                     ISLPGGLKKAISYGKRISSVLENLPDKIFGKNSPDEIVSQVMEEALKEH"
     gene            3563..4870
                     /locus_tag="PLR13_07140"
     CDS             3563..4870
                     /locus_tag="PLR13_07140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQC19150.1"
                     /translation="MSQQIFSLSSWPRAIVHIDGDAFFTSCEEAIHPELRGKALITGG
                     ERGIVACASYPAKKLGIKRGVPLAEARKICPGLIVLPSDYETYSLFSQRMFNIMRRFT
                     PDVEEYSIDEAFADLTGMRRLLRSSYEEIVLKMKKEIERELGITVSVGLSITKVLAKI
                     ASKHQKPSGVTIIKGRDIASCLGNLPVEKIWGIGPATTNYLAKMGIRYALEFAQLPEK
                     KVREKFTKPGVEIWQELRGESVYPVTAEEKSSYRSISKTKTFAPPTSNADYLFAHLMR
                     NMESACIKARRYCLAPKKIVVFLKKNDFNTVGSEVKLSRPCAFPMEFSGVIRDLFDQC
                     YSVQDIYRATGVVLCELEPDEQVQYSLFDNPLQAEKIRSVYNVADELGRKFGKHALHL
                     GSSHPIDTLGRGRRGNPTVREQTRLKGETRRRHLSLPLLHVKT"
     gene            complement(4902..5462)
                     /locus_tag="PLR13_07145"
     CDS             complement(4902..5462)
                     /locus_tag="PLR13_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19151.1"
                     /translation="MNVKGTVFLTGKVAITANFGEARWNEFMAKLAEKDPFFKNVIMS
                     VTLVPVDKHLFFLDEMLKEFFRNDKSQFLLFGRVAAKFALSPGGPYHSYLLTKDIKQF
                     VESGMPKLWSTYYDGGKFTAKLENNIVHLKISDIPIKHIYFEYLIMGYFKQALKIFGK
                     ENIEKRVRSIASGDDDIYYQYEIKNL"
     gene            5819..6736
                     /locus_tag="PLR13_07150"
     CDS             5819..6736
                     /locus_tag="PLR13_07150"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HQC19152.1"
                     /translation="MTLKDIKKVLILGAGTMGQQIGFLCAMHGYNVTMYDISEDILKA
                     SVARMEKLGSSWFVRINRITPEQLKEIMSRITATSDAKTAARNADLISESVPEDPKLK
                     GKIFAQFNTLCPKRTIFTTNTSLLMPSKFAEATGRPGKFAALHFHDVRTTNVVDVMPH
                     PGTDKAVTTLVHDFAVSIGQIPIMLHRESNGYVFNAMLSNLFMTALNLVTKDVASIED
                     VDRAWMGITMMPLGPFGIMDQVGLKTVWTITDYWADKMKDPQSKANANFLKSYVERGE
                     LGAKTKKGFYSYPRPAYSHPEFLTGQIKS"
     gene            6905..7231
                     /locus_tag="PLR13_07155"
     CDS             6905..7231
                     /locus_tag="PLR13_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19153.1"
                     /translation="MAEDDDVLTTDDNESPVSGYLSIVLTIIGLVMLLLIKILGITSS
                     DETGWSIEFVLYLFISMTFAIAGGFLGFLGLSEDNNKKIFPIIGLIVAAIYVVSLGYV
                     IATSIS"
     gene            complement(7444..8415)
                     /locus_tag="PLR13_07160"
     CDS             complement(7444..8415)
                     /locus_tag="PLR13_07160"
                     /inference="COORDINATES: protein motif:HMM:NF021550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HQC19154.1"
                     /translation="MKKCKDIENNLSLYLDDVLSKEDKAAVDEHLRSCPQCAEALARL
                     RKTKELTNSLHEVEPPPWFRKKIMARVREEAGKKSFLYKLFYPLQIKIPVQIFATVCI
                     AVLAVYLYRAGENRFQEVVPSSVPAPVLQTQQDQSPEAKENTSLAERTQPEPNTISQK
                     DKPRESVRKKEVHRVTDVHETDVNELKASDGRDDSNKIAPAQVPEQFSVESAQTKDRH
                     VSGTALKASVAPRAQDSHIKPGIVLKVTDLEVAVREAEQIFRQCEAKNITATIMRDKA
                     FLTGELNRHNIKDLLRQLSTIGRVEEKHIPAADAEGNTALVVEITNR"
     gene            complement(8412..9032)
                     /locus_tag="PLR13_07165"
     CDS             complement(8412..9032)
                     /locus_tag="PLR13_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQC19155.1"
                     /translation="MSNIINTRAKTAKDEDLKYVTLCQKGDTESFDVLVERHQKKMLN
                     IAYRMIGDYDEACDVTQEAFVAAFKSIRQFKAEAKFSTWLYRIVVNYSKNRLKQLSGR
                     SRREGFSIDATGGRNADQLSDQGMSQESNPGVQMEKREREAYVQKCINTLDAEHRDVL
                     ILRDIQGFSYEEISAILKVPDGTVKSRLSRARNALKNCLQKVIGDL"
     gene            complement(9138..10085)
                     /locus_tag="PLR13_07170"
     CDS             complement(9138..10085)
                     /locus_tag="PLR13_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19156.1"
                     /translation="MKSIMQIECPHCHFEFKSEDIAHTLHGGNFKCPVCGKKIPATPA
                     PKNNSSRVLRGKYYVIILMAIGILAALIFYHFTKPPRQATPKPVSAAPAAAESPAALP
                     PVQVFTPSDAPPPPEAIPPLPTRQASPKPDKMKMVEQIAAEFHKGHTYTLEGEFVCLD
                     MAIDVWNQLVTNGIEAKIMGGTLHESITAWNFRQLAMGSNHAWVVATVGPMEKVAIET
                     TSGTVIKPGMKEYASYFKGIEFDSPAQIKRFELLRRKMNDVCRDAHQMIQDWNQNVAG
                     KQLHPAEIVARQSRLEQRKQDCENTFRELREFESKAIFY"
     gene            complement(10100..10564)
                     /locus_tag="PLR13_07175"
     CDS             complement(10100..10564)
                     /locus_tag="PLR13_07175"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQC19157.1"
                     /translation="MDAVPIDVILEQLKIVYEEKMPFNKYLGLKIDSIKADKAVVRID
                     MRDDLIGNFEKNILHGGVISTILDFTGSVIAQIHIINEMKDAPLEKIVKRLTGMGTID
                     MRIDYMRPGKGSYFLATGHLLRLGHKVAVARTEFVNDSGELIAVGTSTYIVG"
     gene            complement(10608..11897)
                     /locus_tag="PLR13_07180"
     CDS             complement(10608..11897)
                     /locus_tag="PLR13_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQC19158.1"
                     /translation="MSINVKKNSKTWVVYILRCCDGSLYTGITNNIEKRLAAHHQGKA
                     AKYTRSRRPVELLVTGTAMERSAAMRLEIKIKRQPKDKKVSALKKHAPRMSSTAATTS
                     KRRVVAAGEHSLFGKETLKAVAGLWQVPLKKIHRKIPVQGSPERSAFRVVLEDTDKRF
                     FVLEQIASQSAAGRKQIAAILDLLAAKKLPQINPYLTDERGNYLLEHQNNFWQLSPFV
                     PGVELDREKYVFEPWRGIVLADFLIALRRKSQKMTLAGSRGVFSLRNYIYKLVREINL
                     YNKDIKNDIGSVVDFLEKDFLPVYEELPVAFCHGDYHPLNIIWSTDGIRCVIDWEFCG
                     LKSELYDVANLIGCIGMENPRSLAGGLVGHFIDRMKRSGIMSQISWRYLTEFIIALRF
                     AWLAEWLRRKDRNMIRLERDYMKLLMDNKTVLQKAWL"
     gene            12248..13525
                     /locus_tag="PLR13_07185"
     CDS             12248..13525
                     /locus_tag="PLR13_07185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQC19159.1"
                     /translation="MSFEKFELDAKILAGVQACGYNVPTPIQLQSIPPIMEGRDVMGL
                     AQTGTGKTAAFVLPILQRLMQGARGHVRALIIAPTRELSEQTHTAIMQLGRKTKLRSV
                     SIYGGVNINGQINKLRNNVEIISACPGRLLDHINRGTVDLSRIEVLVLDEADHMFDMG
                     FLPDIRKIVKHLPAQRQTLLFSATMPDDIRSLANDLLINPVNIKIGHTAPVETVSHKF
                     YPVQMSSKTDLLKDILDKTDMDSVLIFTRTKSRAKSVALQLGKSGYKVTSLHGNLTQQ
                     KRQHALDGFRDGRYEIMVATDIAARGIDVNSISHVINYDMPSTTDAYTHRIGRTGRAA
                     KTGDAFTFVTGEDRGLAQSLERVLGKKLHYQNVDGLTMSLPVDDRVSASRNSRGRNMK
                     PHNDRKKFSPKARANSRLNRRSFDSSNRSQWAH"
     gene            complement(13618..14073)
                     /locus_tag="PLR13_07190"
     CDS             complement(13618..14073)
                     /locus_tag="PLR13_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQC19160.1"
                     /translation="MRKKERAIKDRKDIDGIIRRCRVCRLAMSDEDQPYIIPLNFGYD
                     GFCLYFHAAPEGRKIDILKRNNRVGFEFDILHEILDAAQPCAWSAKYESVIGSGTAEI
                     INDVKGKKAALECIMRQYGSDAGDFSEKVMKKTLIIRVRIREISGKARR"
     gene            complement(14118..14957)
                     /locus_tag="PLR13_07195"
     CDS             complement(14118..14957)
                     /locus_tag="PLR13_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQC19161.1"
                     /translation="MTKHLFYPVKASIKKSYIHGYDPVENIRLQDQASTLVELLHVDT
                     AYAPGSTVLEAGCGVGAQTITLAKNSPGALITSIDISAASIDEARRKTEAAGIQNVHF
                     EQADIFNLSFASQSFDHIFLCFVLEHLPQPVEALIILKRYLKKNGTVTVIEGDHGSAY
                     FYPDSAAAHKAIESQVELQRRAGGNANIGRALHPLLNQAGFRSVCVSPRMVYVDSGKP
                     QLVDGFIKKTFTAMIEGVRAEAIDAGIIGKNVFDQGIRDLYRTAEPDGLFCYTFFKAT
                     GYK"
     gene            complement(14979..15845)
                     /locus_tag="PLR13_07200"
     CDS             complement(14979..15845)
                     /locus_tag="PLR13_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQC19162.1"
                     /translation="MRWRGERQSDNVEDRRGMSISRGAKVGGIGGLGLVAIVVISMFM
                     GVDPSSILQVVEQNTQSSSISVEQSATTAANDNARNFVAVVLAETEDVWNDVFQQAGR
                     TYEEPKLVLFSEAVESACGMAGSATGPFYCPADHKVYLDLAFFEELHSRFGASGDFAQ
                     AYVIAHEIGHHVQTQLGIIQKVHELQSRTSKSEANKLNVKLELQADCLAGMWAHQADK
                     KRNILEAGDIEEGLNAASAVGDDRIQKQTQGYVVPDGFTHGSSAQRVTWFKRGFEQGN
                     IGACDTFNAARL"
     gene            complement(16114..16632)
                     /locus_tag="PLR13_07205"
     CDS             complement(16114..16632)
                     /locus_tag="PLR13_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQC19163.1"
                     /translation="MVKLYRCIICGDAYIGASPPANCPFCGAHMEYIMEAKDAVVNFD
                     VPLSAKDRANAEHALKVEISNATFYSCAAGKTDDPEGKVLFKALGKIEAEHASIWKKI
                     LKLASLPPGNETCHTENVGNLKESHARETRAIEFYRKSAAEADHPRIRQLFEALVEIE
                     TDHLFLSEERLH"
     gene            complement(16668..17045)
                     /locus_tag="PLR13_07210"
     CDS             complement(16668..17045)
                     /locus_tag="PLR13_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HQC19164.1"
                     /translation="MMANRNEVYKCNVCGNIVEILHAGVGKLVCCDQPMKLMTENTTD
                     AAKEKHVPVVEKVAGGFKVTVGSVAHPMEEKHYIEWIEVIADGKIYRQYLAPGQAPEA
                     VFPIEAISITAREYCNLHGLWKA"
     gene            complement(17068..17565)
                     /locus_tag="PLR13_07215"
     CDS             complement(17068..17565)
                     /locus_tag="PLR13_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQC19165.1"
                     /translation="MSKTEEALKEAFAGESQANRKYLAFAAKADQEGFAQAAKLFRAA
                     AEAETVHAHAHLRAMKGIRSTKENLQEAIAGETHEFKSMYPAMIEAAKAESHKEAERS
                     FNFANEVEKIHAALYQKALDHLDGSQETYSYYVCPVCGYTVENEAPETCPVCGAKGKM
                     FKKVD"
     gene            complement(17686..19893)
                     /gene="katG"
                     /locus_tag="PLR13_07220"
     CDS             complement(17686..19893)
                     /gene="katG"
                     /locus_tag="PLR13_07220"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQC19166.1"
                     /translation="MTEESKCPVTGMMKKPISGGGKSNRDWWPNQLNLKILHQQNKKS
                     NPMGEAFDYAKEFKKLDLKAVKKDLITLMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RMGDGRGGAGTGNQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNC
                     ALESMGFKTFGFAGGRVDVWEPEEDIYWGSEEEWLATSDKPKSRYSGERDLENPLAAV
                     QMGLIYVNPEGPDGNPDPIASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGPA
                     THVGPEPEAAPLEAQGLGWGSSYKSGKGGDTISSGLEGAWKPNPTTWDMGYLKVLFKY
                     EWELVKSPAGANQWLAKNVEDEDMVADAHDPSKKRRPMMTTADLSLRFDPIYGPIARR
                     YLANPKEFADAFARAWFKLTHRDMGPRSRYLGPEVPKEVLIWQDPIPAVNHKLINKAD
                     ANSLKSKILASGLSISELVSTAWASASTFRGSDMRGGANGARIRLAPQKNWEVNEPER
                     LAGVLKTLERIQKAFNKDQKGDKKVSLADLIVLAGCAGVEQAAKNAGYKITVPFTPGR
                     MDASQKQTDVASFGVLEPKADGFRNYQKAKYTVLPEEMLVDKAQLLTLTAPEMTVLIG
                     GLRLLGANFGGSPHGVFTSRPGTLTNDFFVNLLDMGTEWKVSKDADVFEGRDRKTGEL
                     KWTGTRVDLIFGSNSQLRAVAEVYACKDSQEKFLDDFVAAWNKVMNLDRFDLA"
     gene            complement(20058..20486)
                     /locus_tag="PLR13_07225"
     CDS             complement(20058..20486)
                     /locus_tag="PLR13_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQC19167.1"
                     /translation="MIYKDGLIGQLKEKGLKITPQRLAIIDALVEHSEAHPGATLIYE
                     AASKKARRISLSTVYATLKEFSENGLIRQLEFDRMENRYDVDLSDHVNLICNRCGKIF
                     DYAIPASLEPRDIARKSGFVVTETRLEFHGYCRDCLKRSD"
     gene            20633..20962
                     /locus_tag="PLR13_07230"
     CDS             20633..20962
                     /locus_tag="PLR13_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19168.1"
                     /translation="MPVYLQVILLVAIFIATVIIFLYIGGLAVRRASFQIIAEMEEAR
                     AFKEGRAIKIQDERKNFFRVGTKNIRPRALNLLIADGLVIKTNNGKYYLDKEKLAQAK
                     AQLSSKT"
     gene            complement(20984..22129)
                     /locus_tag="PLR13_07235"
     CDS             complement(20984..22129)
                     /locus_tag="PLR13_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19169.1"
                     /translation="MNRKTELEEITHTGGKVNFNIKIDADGRIAYNVGLTHSRPTPAA
                     LFAVYAIPQGVAVHDIKMGGIGTPWNPPPLPDCFPVFISSDSTGMFGHQCPSCNGYWR
                     AGLGGKICPYCAFTADEYYHFLTDAQQRYVRQYCEVLSNALASGQSGEHIIDMDSVAE
                     AAGKDCEKPAFYYAEERQQNLFTCNACGKVNDVLGTYAYCSSCGTRNDLQELEKNIQQ
                     IRDRINAGGPYETCVKETVAIFDSFAGHYAKQLLTRVPLTPARKAWIEKSHFHNIGTV
                     SEMFRKVFDIDVLSSIDSEDVSFGTLMFHRRHVYEHKGGEADEKYITDSGDSVRLKQA
                     LRETKETAHRTANFITKIATNLHKGFHEIFPPLEEPIKQYEQRKYKR"
     gene            complement(22401..>23078)
                     /locus_tag="PLR13_07240"
     CDS             complement(22401..>23078)
                     /locus_tag="PLR13_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQC19170.1"
                     /translation="LLDRSEAYMAVMIDDLVTRGVDEPYRMFTSRAEYRLILREDNAT
                     IRLMGKGCELGLIDKTHYLELQDKIKKIENGIEQLKASPVKPTAETNEKLATLNSPPL
                     SNHTTLHGLLKRHELSYDNLSIFPGWEPELDAFVKQQIEIETKYEGYIKRQFDAVKKL
                     KDQEKKKIPPDFDYNSVPGLSNELKLKLTKIAPQTLGQMERIPGMTEGAISAVLIMMK
                     KHELQGR"
BASE COUNT         5493 a         5697 c         5632 g         6256 t
ORIGIN      
        1 ttcgttggcc agttgaaata taatgaggaa gctggtacat gaagcccatg aatatagatg
       61 acatactgaa ggatttaact gagcgtgccg gctcggctgt ggcccactat tggaaaacgc
      121 gtgcaggaca gagggacaag cagaggcttg gcggaacggc cgatcagggg ctgcgtagtg
      181 ctgtgacagg cggcgctcag atgaatggtt ttattgacct ttttacggaa tttattgtcg
      241 cggttggcat acccgagcaa tgtgtatttc gtaaacgggc tgttgaattg ccgggattct
      301 ttcgtcctac caaagaatgg gatctgcttg tcgtgcggga aaacaagctt cttgctgtga
      361 tcgaggcgaa atcgcaagtc ggcccctcat tcggcaataa cttcaacaac cgaactgaag
      421 aagccatggg aagtgctctt gatctgtgga cggcctaccg agagcgagcg tatcttgata
      481 gtccccagcc ttttctcggt tatttcttca tgctggagga ttgcgatgcc tccaataggg
      541 ccgtgaaggt acaggaaccg cacttcaagg tgttcccaga attcgtcggc gcttcgtata
      601 agctgcggta tgagctattc tgtcggaagc ttgtgcttga acgccattat acagcaacag
      661 cgtttattgc ctcgacacag gatgatggca tccttggcca tttttcgaca ccagcagacg
      721 acctttcgct tgtgcgcttc gccagagtcc ttatcagcca tcttgcggca tttgtgtaac
      781 ggcatggaag aaacgactca tagactgatt aacggtgacg cgcgggagct atccttcctg
      841 catgatgaat cagtccatct tgttgttacg tctccgccct attggagtct caaacgctac
      901 aacgaacacc ccgatcagct tggacacatt caggattacg aggcatttct gtttgaactg
      961 gaaaaggtct ggcggcacgt ctaccggatt cttgttcccg gagggcgtct cgtttgtgtc
     1021 gtcggggatg tctgtgtcgc tcgacgtaat ttcggacggc accttgtctt tccactgcat
     1081 gcggatatct gcgttatttg tcggcgaatc gggttcgata atctgaatcc gattatctgg
     1141 cacaagatcg ccaacgcttc atacgaggtc tccaacggat cgaagtttct cggaaagccc
     1201 tacgaaccaa acgccatcat caagaatgat atggagttta ttctcatgca gaggaaaccg
     1261 ggcggttatc ggcaaccgac caatatgcaa agagatgcaa gccgcattga caaagaagag
     1321 tttaaccgct ggtttcagca gatttggaac atcaccggcg cgtccaccaa acagcacccg
     1381 gcgccattcc cccttgaact tgcaacccgg ctcgtacgta tgttctcatt cacgggcgac
     1441 acggtgtttg atcccttctg tggttcaggg accacgatga ttgcggcact gcgaacaggt
     1501 cgtaacagta tcggaattga tatcgatcca gaatactgtc ggatggccgc acggtatttg
     1561 aaggcggaga acgccgacct gtttgcaacc acgaaacttg tctttgagaa agctaccgcc
     1621 gaacctgcca gcctggtgaa ggaagacctt gccctctacg aagtgcgacc ggcgagaaag
     1681 aaattggcat aggccaacaa acccatgcaa gcgacgcctg aaggcgcgcc tgatgggtag
     1741 cgttagaaaa taaggatcag atatggcttt tagtgagata gaactgaaga gaatcgagaa
     1801 agaagtgggc gatttttgtg aaaagcgaag tcctgttgaa attcgtgata aattgagttt
     1861 ccaatatcgt attaaagcac acgatgtcat catttatacg cgccgtcccc gatgggataa
     1921 tcccaaagaa tggatggaaa gtgatatggc aaaaattaag tacgtgcgca cttctaatat
     1981 gtggcagttg ttttggcaga gggcgaatgg caaatggttt agctatgagc cttttcctca
     2041 tagtaaaaat ctcaagtcaa ttattgaaga gattgaggca gatcaatacg gctgcttctt
     2101 tggctaataa aaatcgcata accaggcgct agagccgatc gcttgcgctc cggcttgttt
     2161 tatcgttaga cgaaaacgat aaagggagat atccatgaaa aagatgcaaa tggataaagc
     2221 atttacgctg atggagtccg gtcccgtcgt tctcattaca accaatgacg gaaaaaagaa
     2281 taatatcatg acgatatcgt ggaccatggt ggtggatttt acaccggtat ttgcgataac
     2341 taccggatca tggaactatt cctttgccgc tcttcaaaaa acgaaggagt gcgttattgc
     2401 cattcccacc gttgacatgc ttgataaagt cgttggagtg ggaacctgtt ccggctccga
     2461 tacggataaa tttgaaaaat tcatgctgac ccccgtcaag ggaaagcatg tcaaagcgcc
     2521 cctgataaaa gaatgcctgg cgaatatcga gtgtaaagtt gccgatattg tacgaaagca
     2581 caacatcatc gtgcttgatg ccatggccgc ctacatcgac gataagcgga aagagaaacg
     2641 agcgttgcac gccgttggcg atggcacgtt tattgtcgac gggcgtaaaa tggaccggag
     2701 gaagatgatg gcctccaagc ttcccgcagg cgtctagctt gtgtgattcg cctcactggt
     2761 cgcgccacgc accggctgaa ctttttgtta acggggttat tgacatgaaa aaatttagaa
     2821 tttactgctc tggtccgctg ttctgcgcag aagaaatcgg cggtatgagt gccattgcca
     2881 aagtactgga cgatgcaggc tttcaaacct tcttgccgca tcgtgacgga ctggaatcgt
     2941 atatcctgcg tttttcaaat gcatcgtttc catccaccat ttcagcgata cggacacggg
     3001 ttgattacgc tatattttca ttggatgcct atgaactgat tgaaagatgc agcgctgtcg
     3061 tatgcaacct caacggccgt gttccggatg aaggaatgat tgttgaagca tcccttgcat
     3121 atgccgccgg caagcctctt gtctttttca aaaatgatct cagggctcct tttggcggat
     3181 acgataattc catgctgacg agtctggtgc gcggaaaaat tgtcaaaagg ctgaaggaat
     3241 tgccttctgc gcttgaatat gaattatcgc gggaaccgga attaatatcc ctgccgggcg
     3301 gtttaaaaaa agccatctca tatgggaaac gtatctcatc ggtattggaa aacttaccgg
     3361 ataagatatt cgggaaaaac tcgccggacg aaatcgtcag ccaggttatg gaagaggcgc
     3421 tcaaagagca ctgatcgtcg ctaatccgcc ggacggcttg cgactgctga tcggcacgtt
     3481 agggcttcca ccttccacga catcaaaaaa tgaagttttt ccgggttttt caatttttcg
     3541 tttttcctgt ttaaaaggtt ttatgtctca gcaaatattc agtctttcct cctggccgcg
     3601 cgcgattgtg cacattgacg gcgatgcttt tttcacgtcc tgcgaggaag ccattcatcc
     3661 ggagctccgg ggcaaagcgc tgattacagg cggcgagcgc ggtattgtcg cctgtgccag
     3721 ttatccggcc aaaaaactgg gcatcaagcg cggcgtgcct ctggctgagg cgcgtaaaat
     3781 ctgcccggga ttgattgttc tgccttcaga ctacgaaacc tacagcttgt tttcgcagcg
     3841 gatgttcaac atcatgcggc gtttcacgcc tgacgtggaa gaatattcca tcgatgaggc
     3901 gtttgccgat cttaccggca tgcgacgatt gctgcgttcc tcctacgagg aaattgtact
     3961 gaagatgaaa aaggaaatcg aacgcgaact gggcatcacg gtttctgtcg ggttgagtat
     4021 caccaaggtg ttggcgaaaa ttgcctccaa acatcagaag ccatccgggg tgaccatcat
     4081 caagggaagg gacattgcct cttgtcttgg aaatcttccc gtggagaaaa tctggggcat
     4141 cggtcccgcc acaaccaact atctggcgaa gatgggcatc cgctacgcgt tggaatttgc
     4201 acaactgccg gaaaagaaag tgcgggaaaa atttaccaag ccgggcgtgg aaatctggca
     4261 ggaattgcgc ggggaatcgg tttatccggt gacggcagag gagaaaagtt cttatcgttc
     4321 catcagcaag acgaaaacct ttgcgccgcc gacgtccaat gccgactatc tgttcgcgca
     4381 cctcatgcgc aatatggaat cggcgtgtat caaggcccgg cgctactgcc tggcgccgaa
     4441 gaaaattgtt gtctttctca agaaaaacga tttcaacacc gtcggcagtg aagtcaagct
     4501 gtcacgcccc tgtgcttttc ccatggagtt ttccggcgtt atccgtgacc tgtttgatca
     4561 atgctactcg gtacaggata tctaccgcgc caccggtgtc gttttgtgcg agctggaacc
     4621 ggacgagcag gtgcaatatt ctctttttga caatcctctg caggcggaaa aaatcagaag
     4681 cgtttacaat gttgccgatg aactggggag gaagttcggc aaacatgcgc tgcatctggg
     4741 aagctctcat cccatcgata cgttggggag aggccgccgg ggtaatccga ccgtccgtga
     4801 acaaacccgg ctaaaaggcg aaacccgccg ccgtcatttg agtctgcctc tgctgcacgt
     4861 caagacatag agaattttat gtgaacgtaa cggatttttt atcagaggtt ctttatctcg
     4921 tactgataat aaatgtcgtc atcaccgctg gcgatacttc tcaccctttt ctcaatattt
     4981 tctttcccga atattttaag ggcttgctta aagtacccca tgatgaggta ctcgaaataa
     5041 atatgcttga tgggaatatc gctgatttta agatgaacga tgttattttc caactttgcg
     5101 gtaaacttgc cgccgtcata ataggtggac caaagtttgg gcatacccga ttcgacgaac
     5161 tgtttgatgt cttttgtgag aagataagaa tgatagggcc cgccgggaga cagggcgaat
     5221 tttgccgcta ccctcccaaa cagtaaaaac tggcttttat cgtttctgaa gaactctttc
     5281 agcatttcgt caagaaaaaa aagatgcttg tctaccggaa caagcgtgac agacatgata
     5341 acatttttga aaaacggatc cttttccgca agcttggcca tgaattcatt ccagcgcgct
     5401 tcgccgaaat ttgctgtgat agcgactttt cctgtcagaa aaacagtacc ttttacattc
     5461 atattaaacc ctccccggag tcgttgattg gcttaaaaat aagatgagtg atggtcacga
     5521 atatgtaatg gcgttttttc atcggaagca attaattttc gcatcatgcc ggattcttac
     5581 gctttaccgt tacatcaaag gctgcgccgc tggacgcctt gcccgtgaca gaaaccgtac
     5641 ttttgcgttc atgtcacctt ccaaagtata tgtgtatgag ttaatcctta tattttgtgt
     5701 tgcataaata ttcaagtaca atattatttt tttcccattg cgtcttcaca ttttcccctt
     5761 ttgaaaaaca taaccccatg atattataac ttagagttat ttgaagaaag gaaaatttat
     5821 gacattaaaa gacattaaaa aagtactcat tctgggagcc ggcaccatgg gacagcagat
     5881 cggtttttta tgtgccatgc acggctataa cgtgaccatg tatgatattt cggaagacat
     5941 tctgaaagcc tctgtcgcgc ggatggaaaa actcggttca tcatggtttg tccggatcaa
     6001 caggattacg ccggagcagc tcaaggaaat catgagccgt atcacagcaa cgtctgatgc
     6061 gaaaacagcg gccaggaatg ccgacctgat cagtgaatcc gttccggaag atccgaaact
     6121 caaaggcaag atttttgctc aattcaacac actctgcccg aaaaggacta tttttaccac
     6181 gaacacctcg ctgcttatgc cgtcaaaatt tgctgaagcc accggcaggc cgggaaaatt
     6241 tgccgcgttg cattttcatg atgtgcgaac aaccaatgtg gtggatgtta tgcctcatcc
     6301 gggaacggat aaagccgtga ccacactggt tcacgatttt gccgtcagca tcggccagat
     6361 tcccatcatg ctgcatcgcg agagtaacgg gtatgtgttt aacgcgatgc tttccaatct
     6421 tttcatgaca gccttaaacc tggttacgaa ggatgtggca agtattgaag atgttgatcg
     6481 cgcctggatg ggcattacca tgatgcccct tggaccgttc ggcatcatgg atcaggtcgg
     6541 tttaaaaacg gtttggacca tcacggatta ctgggcggat aaaatgaaag atccccaatc
     6601 caaggccaat gcaaatttct taaaatctta tgtcgaaaga ggagaactgg gcgccaaaac
     6661 gaaaaaaggt ttttattcct atcccagacc ggcgtacagt catccggaat ttctgacggg
     6721 acagatcaaa agttgatatc ggtacgggag ggttgatatg gatccgtttc aagacgttca
     6781 tcatcccgat attgaatccg tccttcaggt ttcacggtca atatttcatt gactctcttt
     6841 cttgtttttt ttataatcat ctcagggccg ttttaagagg tcaatacaac gaggaggaag
     6901 caaaatggca gaagacgatg atgttttgac gacggatgat aatgaaagtc ctgttagcgg
     6961 ttacctttcc attgtattga caatcattgg attggtgatg cttttgctta ttaaaatact
     7021 gggcattacg tcctccgatg agaccggctg gtccattgaa tttgtattgt atctttttat
     7081 cagtatgact tttgctattg ccggcggatt cctcggtttt ctgggattat cagaagacaa
     7141 caataaaaaa atatttccga ttatcggttt gattgtcgcg gccatatacg tcgtttcgct
     7201 cgggtatgtt atagccacgt ctatttcgta aatgtttctg aaaacaataa cgtttctgat
     7261 ccgctgctgc ttgaattttt attgcaggtc gcagcggatt cttttctgca gacaatcccc
     7321 tgatctttca taaatatcct gtgccggtta tcgaggcaac aggcatgttt cccggccggc
     7381 catacggagg cggaaaatct gataaacggg aattgatctt aaagccaacc gtcgcgagac
     7441 cgtctagcgg tttgtgattt ccaccaccaa agcggtattg ccctcggcat cggctgccgg
     7501 aatatgcttc tcctccaccc ggccgatggt cgataattgc ctcaacaagt cttttatgtt
     7561 gtgacggttg agttcacccg tcaagaaggc tttatcccgc atgattgtcg cggtgatatt
     7621 ttttgcttcg cattgtctaa atatttgttc tgcttcccgc acggcaactt caagatccgt
     7681 aaccttcaat acaatgccgg gttttatatg tgaatcttgc gcccgtggcg cgacgcttgc
     7741 cttcagggct gtccccgaaa cgtgacggtc ttttgtctgt gcagattcca cactgaattg
     7801 ttccggcacc tgtgcgggag cgattttatt tgaatcatcc cgcccgtcgg aagccttcag
     7861 ttcattgaca tccgtttcat ggacatccgt cacccggtgc acttcttttt tacggacaga
     7921 ttcccgtggt ttgtctttct ggctgatagt gtttggttcc ggctgtgttc gttcagcgag
     7981 ggatgtgttt tctttggctt ccggcgactg atcttgctga gtctgaagca ccggcgccgg
     8041 tacggatgaa ggaacaactt cctgaaaacg gttttccccc gcccggtaga ggtaaacggc
     8101 aagcacggca atgcagaccg tggcaaatat ctgaacggga atttttatct gcaggggata
     8161 gaataattta taaagaaaac ttttctttcc ggcttcctcg cgcacccggg ccatgatttt
     8221 tttccgaaac cagggcggcg gttcgacctc atgaaggctg ttcgtcagtt ctttcgtttt
     8281 gcgcagccgg gcaagtgcct ccgcgcattg cggacacgat cgcagatgtt cgtccacggc
     8341 tgctttatct tctttggaaa gcacatcgtc cagatacaaa gacaggttgt tctcaatatc
     8401 cttgcatttt ttcataagtc accaataacc ttttgcaggc aatttttcag cgcgttgcgg
     8461 gcccttgaaa gccgtgattt aaccgttcca tcaggcacct tgaggatggc gcttatttct
     8521 tcataagaaa agccctgaat gtctctcaat atcagcacgt cccggtgttc tgcatccagc
     8581 gtgttgatgc atttctggac atacgcttct ctttcgcgtt tttccatctg tacgccggga
     8641 ttactctcct gactcatccc ctgatcggag agttgatcgg cgtttcttcc cccggtggcg
     8701 tcaatggaaa atccttcgcg ccggcttcgg ccgctcaact gctttaagcg gtttttggaa
     8761 taattgacaa cgattcgata gagccaggtg gaaaattttg cttccgcctt gaactgccgg
     8821 atggatttga aggccgcgac aaaggcttcc tgggttacat cacaggcttc atcgtaatcg
     8881 ccgatcatcc ggtaagcgat gttgagcatt ttcttctgat gcctttccac cagaacgtca
     8941 aaagactctg tatcgccttt ttgacaaagc gtgacatatt tcagatcttc atctttggcg
     9001 gtctttgccc tggtgtttat tatattagac atccatagca atgcttttgt tccccttcta
     9061 tgtggctcat acagagctca ttttaccgtg atcttgcgtg ggaagccggt tgtgtgcatt
     9121 cggatacgag aggcggatca gtaaaaaatc gctttggatt cgaattccct caattccctg
     9181 aacgtgtttt cacagtcctg ctttctctgt tccagacgcg actgcctggc gacaatttcc
     9241 gccggatgaa gttgtttgcc ggcgacattc tggttccagt cctggatcat ttgatgggca
     9301 tcccggcaga cgtcattcat ttttctccgg agcagttcaa atcgtttaat ctgagccggc
     9361 gaatcgaatt caatgccctt gaagtaagaa gcatattcct tcatgcccgg tttgatgacg
     9421 gttccggatg tggtttcaat ggcgactttt tccatcggac cgacggtggc caccacccag
     9481 gcgtgattgc ttcccattgc caactgccgg aaattccacg cggtgatact ttcgtgcagg
     9541 gttccgccca ttatctttgc ttcaatgccg ttggtcacca gttgattcca gacatcgata
     9601 gccatgtcca gacaaacgaa ttcgccctcc agggtatacg tgtgcccctt gtgaaattcc
     9661 gcagcaattt gttccaccat tttcattttg tccggtttgg gtgacgcctg ccgggttggc
     9721 aggggcggta tggcctccgg aggaggtggc gcatcggacg gtgtaaaaac ctgcacgggt
     9781 ggaagagctg cgggagattc cgctgccgcc ggtgcggcgg aaacaggttt gggagtcgcc
     9841 tgacggggag gttttgtaaa atgataaaaa atcagtgcgg ccaatatgcc gatcgccatc
     9901 agaattatta cataatattt gccacggaga acccgtgatg aattattttt tggagccggt
     9961 gtagccggta ttttttttcc gcagaccggg catttgaaat tacccccgtg cagcgtatgc
    10021 gcaatgtctt cggacttgaa ttcgaaatgg caatgcgggc attcaatctg catgatgctt
    10081 ttcatttcct tgttacgcgt tatccgacaa tgtaagtgct ggtgccgacg gcaatcagct
    10141 caccgctgtc attgacaaat tccgtacgcg cgacagcaac cttgtgtccc agtcttaaaa
    10201 gatggccggt cgccaggaag tatgatcctt tccccggacg catataatcg atccgcatat
    10261 caatggttcc catgccggtc agacgcttga cgattttttc caaaggcgca tccttcattt
    10321 cgttaatgat gtgtatctgc gcgatcacgc tgccggtaaa atcaagaatg gtcgatatga
    10381 cgccgccgtg taaaatgttt ttctcaaaat tgccgatcag atcatcccgc atgtcgatcc
    10441 ggacgaccgc tttgtcggcc ttaatggaat cgatcttcag accgaggtat ttattaaacg
    10501 gcatcttttc ttcatagacg attttcaact gctcaagaat gacatctatc ggcacagcat
    10561 ccatggtcac ggttatcctg aaaattttaa tgtagagccg ccttttatca cagccaggct
    10621 ttttgtaaaa cagttttgtt atccatcagc agtttcatgt aatcgcgttc aaggcggatc
    10681 atgttcctgt ccttgcgacg cagccactcg gcaagccagg cgaaacgcag cgcgatgata
    10741 aactcggtca gatatcgcca gctgatttgg gacatgatgc ctgacctttt cattctgtcg
    10801 ataaaatgtc cgaccaggcc gccggcaagg cttcgcgggt tttccatgcc gatgcagccg
    10861 atgagattgg caacatcgta gagttcgctt ttcaacccgc agaattccca gtcgatgacg
    10921 catcggatac cgtcggttga ccagataata ttcagaggat gatagtcgcc gtgacaaaag
    10981 gccaccggca gttcttcata aacgggcaga aaatcctttt ccagaaaatc gaccacgctg
    11041 ccgatgtcgt ttttgatgtc tttgttgtaa agattgattt cacggaccaa tttgtaaatg
    11101 taattcctca gggagaacac gccgcgggag ccggccagtg tcatcttttg cgatttgcga
    11161 cgcagggcga tcagaaagtc agccagaaca atgccgcgcc acggctcaaa aacatatttt
    11221 tcacggtcca gttccacgcc ggggacaaaa ggcgaaagtt gccagaaatt attctggtgt
    11281 tcgagaagat aattgcctct ctcatcggtt agatagggat tgatttgagg gagtttcttc
    11341 gcggccagca aatcgagaat tgccgcaatc tgttttcttc cagccgccga ttgcgatgca
    11401 atttgttcca ggacgaagaa tcttttgtct gtatcttcca gaaccacacg aaacgcggaa
    11461 cgttccggac ttccctgaac aggaatttta cgatgtattt ttttcagggg aacctgccac
    11521 aggccggcca cggctttcaa tgtttctttg ccgaaaaggg agtgctctcc tgcggcaaca
    11581 acgcgtctct tcgatgttgt tgccgctgtg gacgacatcc ttggcgcatg ttttttcagg
    11641 gcggacactt ttttgtcttt gggctgtctt ttgattttta tttccagtcg catcgccgcg
    11701 cttctttcca tcgccgtacc ggtgaccagt aattccaccg gccggcggga gcgggtatat
    11761 ttggcggctt tgccctgatg atgagcggcc agacgttttt caatgttatt ggtgatgccc
    11821 gtatacaggc ttccgtcgca acagcgcaga atataaacaa cccaggtttt gctgtttttc
    11881 ttaacgttga tactcatgac ctgtattttc tacaacagcg ggaaggacat tgccatagaa
    11941 atttacaggc gtatttccga atattcgctt gtttatgagg attaaaagat aatccttgct
    12001 atttatgaat aatacctgta caaggcctca cagtgatcgc gatagttatt gacctccctt
    12061 cctttctact tcagaaattt cggcaggaaa atccggctaa tcgtaacaga atccaacttt
    12121 agcgctagat ttgcagcctg ttttgggctt gtcatcatgg cgcacggcga tgtgttttta
    12181 aacaaccgcg acgaatagac ctgggttcaa atgtcacaaa tactgaaaaa agaaggaaaa
    12241 agaaaaaatg agttttgaga agttcgagtt agatgcgaaa atactggccg gcgtgcaggc
    12301 gtgcggctac aatgtgccga cacccatcca gttacaatct atcccgccga ttatggaagg
    12361 ccgcgatgtg atgggcctgg cccagaccgg tacgggaaaa acagcggctt ttgtgctgcc
    12421 gattttgcag cggctgatgc agggggcacg cggtcatgtc cgcgcgctga ttattgcccc
    12481 gactcgcgag ttgagcgagc aaacccatac ggccattatg caactgggac gaaaaaccaa
    12541 attgcgtagt gtctccattt acggcggcgt gaatatcaac ggccagatca acaaactgcg
    12601 gaataatgtg gaaatcatct ccgcctgtcc cggtcgtctg ctggaccaca tcaaccgcgg
    12661 caccgtggat ttatcccgga tagaagtgct ggttctggat gaagccgacc atatgtttga
    12721 tatgggcttc ctgccggata ttagaaaaat cgtcaaacat cttcccgcgc agcgtcagac
    12781 cttgctgttt tccgcaacca tgccggacga catccggtcg ctggccaacg atctcttgat
    12841 caatccggta aatattaaaa tcggccacac ggcaccggtc gaaaccgtgt cgcataaatt
    12901 ttatccggtg cagatgagct ctaaaacgga tctgctgaaa gatattcttg ataaaacaga
    12961 tatggattcg gttctgattt ttacgcgaac caaatcgcgc gcgaaaagcg tggccctgca
    13021 actgggaaaa tcgggataca aagtaacgtc actgcacggc aatttgaccc agcagaaacg
    13081 tcagcatgcg ctcgacggct tccgcgacgg ccgttatgaa atcatggtcg ccaccgacat
    13141 tgccgcccgc ggcattgatg tgaacagcat ttcccatgtg atcaattatg atatgccctc
    13201 aacgaccgat gcctacacgc atcgtatcgg ccggacggga cgcgctgcca aaaccggcga
    13261 tgcctttacc tttgtaaccg gagaggatcg cggcctggcc caatcgctgg aaagggtgct
    13321 tggcaaaaag ctgcactacc agaatgttga cggattaaca atgagcttgc ccgttgacga
    13381 tagagtttcc gccagccgta atagcagggg aaggaacatg aagcctcata acgataggaa
    13441 gaagttttct cccaaagcgc gggccaacag cagattgaat cggcgttctt tcgattcttc
    13501 caaccgcagt cagtgggcgc attaattcaa tcaaaaaaca ccatcgggtg atcactcttt
    13561 aaaatattta ttttagaagt tatcacccga tggtgaaaga ctgttaattt tcgttctcta
    13621 tcgtcgggct ttcccgctga tttcccgtat gcgcacacga ataatcagag tttttttcat
    13681 taccttttcc gaaaaatctc cggcatcact tccatactga cgcataatgc attccagcgc
    13741 ggcttttttg cccttcacgt cgtttatgat ttcggccgtg cctgaaccaa tcacactttc
    13801 gtattttgca ctccatgcgc agggctgtgc tgcatcaagg atttcatgaa ggatatcaaa
    13861 ttcgaatccg acgcgattat ttcttttcag gatatcgatt tttcttcctt cgggtgcggc
    13921 atgaaaatag aggcaaaagc cgtcatagcc gaagttgagg ggaataatat aaggttgatc
    13981 ctcgtcggac atggccagac ggcacacccg acaacgacgg atgatcccgt caatatcttt
    14041 cctgtccttt attgctcttt ctttttttcg cattttcaat ctgacctgat cggggccgat
    14101 gatgatttgt ccggcaatta tttgtagccg gtggctttaa aaaacgtata gcagaagagg
    14161 ccgtccggtt cggcagtgcg atataaatcg cggataccct gatcgaaaac gtttttcccg
    14221 atgatccccg cgtcgattgc ctctgcacgc acgccctcaa tcatggcggt gaacgttttt
    14281 ttgatgaatc cgtccactag ttgcggcttg ccggaatcca cataaaccat gcggggagat
    14341 acgcaaacag atcggaaacc cgcctgattc agcaacggat gcaatgctct gccgatgttg
    14401 gcgttacctc cggcgcgccg ctgcagttcc acctgactct caatggcctt gtgcgctgcc
    14461 gcgctgtccg gataaaaata cgccgagccg tggtctcctt caataacggt gaccgtaccg
    14521 tttttcttta agtacctttt aaggatgatc aacgcttcaa cgggctgcgg cagatgttca
    14581 agaacaaaac agagaaagat gtgatcaaac gattgcgatg caaaggataa attaaaaata
    14641 tcggcctgtt cgaaatgaac attttgaatg ccggccgctt cggtttttct tctggcttca
    14701 tcaatggagg ccgcggaaat gtcgatcgaa gtgatcaacg cgccgggact gtttttcgcc
    14761 agggtgatgg tctgtgcgcc gacgccgcaa ccggcttcca gcaccgtact gcccggcgca
    14821 taagcggtgt cgacatgcag caattctacc agagtggagg cttgatcctg aagtcttatg
    14881 ttctctaccg gatcataacc gtgaatgtat gattttttta tgctcgcttt tacaggataa
    14941 aaaagatgtt tcgtcatgtc cgcccggtta atcatccttt acaggcgagc ggcattaaag
    15001 gtgtcgcagg cgccgatatt gccctgttcg aagccgcgtt taaaccaggt gacgcgctgc
    15061 gcggaactgc cgtgcgtaaa gccgtccggc accacgtaac cctgcgtctg tttctggatg
    15121 cggtcatccc cgacggcgct ggcggcattc aatccttctt caatatcgcc ggcttccaga
    15181 atatttcttt tcttgtcggc ctgatgcgcc cacatgcccg cgaggcaatc cgcctgcaac
    15241 tccagcttaa cgtttaattt attcgcttcg gatttgcttg tgcgcgactg gagctcatgc
    15301 actttctgga taatgcccaa ctgggtctga acatgatggc ctatttcatg ggcgatgacg
    15361 tacgcctggg cgaaatcacc ggatgcgccg aaacgtgaat gcagctcttc gaaaaatgcc
    15421 aggtcgaggt aaaccttgtg atcggccgga caataaaaag gtcctgtggc cgatccggcc
    15481 atgccgcatg cggattcaac ggcctcggaa aacaatacaa gtttcggttc ttcataggtg
    15541 cgtcccgcct gctgaaaaac atcgttccag acatcctccg tttccgccag caccaccgcg
    15601 acaaaattgc gcgcattgtc attggcggct gtggttgcgc tttgttcaac agaaattgaa
    15661 gaagactgcg tattctgctc aacgacctgc aatattgaac tcggatcaac gcccatgaac
    15721 atgctgatga ccacgatggc caccagaccg agtccgccga tgccgccgac ctttgcgccg
    15781 cgggaaatcg acattcctcg gcggtcttcc acgttgtcac tttgtctttc gcccctccag
    15841 cgcattgttt tctccttttt agggttacat caggaataat atgattacgg cccggacggc
    15901 cggaatcgac ccttatgctt cagttgtcag tatagaaaca aggttttcgg ctgtcaagat
    15961 aattcatctt cgttgatttt attggagaaa atagtcatca atcaatccga tgcggacatg
    16021 attatgcttt aagaaatgta tccggatttg ttcagtaagg gggtgacgcg cagaccgcgg
    16081 aaatacgacc gcgatctgca cgcagccttt gttctaatgc agtctttctt cggacaaaaa
    16141 caggtgatct gtttcgattt ccaccagcgc ttcaaaaagc tgccggatcc gcggatgatc
    16201 cgcttcggcg gctgactttc tgtagaattc gatagcccgt gtttcccttg cgtgggactc
    16261 tttcagattt ccaacattct ccgtatggca ggtctcgttt cccggaggaa gggatgccag
    16321 tttgagaatc tttttccaaa tggaggcatg ttccgcttca attttgccca gcgccttgaa
    16381 caaaaccttt ccttcgggat catccgtttt gcctgcggcg catgaataaa atgtcgcgtt
    16441 gctaatttcc accttgagcg catgttcggc attggcccga tcttttgcgc tcagaggcac
    16501 atcaaaattc acgaccgcat cttttgcctc cataatatat tccatgtgag cgccgcagaa
    16561 cgggcagttc gccggaggac tggcgccgat atacgcgtcg ccgcatatta tgcatcggta
    16621 tagtttaacc atgtgttctc cttttaagat aatgatgcag cgttaactta tgctttccac
    16681 aagccgtgaa gattgcagta ttcgcgcgct gtaatgctga ttgcttcgat ggggaaaacc
    16741 gcctcgggcg cctggccggg cgctaaatac tggcggtaaa tttttccgtc tgcgataact
    16801 tcaatccatt caatgtagtg cttttcttcc atgggatgcg ccacgctgcc aacggtcacc
    16861 ttgaaaccgc cggccacttt ttccaccacg gggacatgtt tttccttggc tgcatccgtc
    16921 gtgttttccg tcatgagttt catgggctga tcgcaacaca caagctttcc cacgcccgcg
    16981 tgaagtattt ctacgatatt gccgcatacg ttacatttgt agacttcgtt tctgttagcc
    17041 atcataaacc tccttgtttt attatgatta atctactttc ttaaacatct ttcccttggc
    17101 gccgcagacc ggacaggttt ccggcgcttc gttttccacg gtataaccgc agaccggaca
    17161 aacataatag ctgtatgttt cctgtgaacc gtccagatga tccagcgctt tctgatagag
    17221 ggcggcgtga attttttcca cttcgttggc aaagttaaaa gaccgctccg cttctttatg
    17281 actttcggct ttggccgctt caatcatggc cggatacatg cttttgaatt cgtgggtttc
    17341 tccggcaatg gcttcctgaa gattttcttt tgtggaacgg atgcctttca tcgccctcaa
    17401 atgggcgtgg gcgtgtacgg tctccgcttc cgctgccgcc cggaataatt ttgccgcctg
    17461 ggcaaatcct tcctgatcgg ccttggctgc aaacgccaga tatttccgat tggcctgtga
    17521 ttctcctgcg aaagcttcct ttaatgcttc ttctgttttg gacattttca tatgctcctt
    17581 cgttttattt ttttatgaat caattaaaaa tttgtcggta aaggcatcac actccgaatt
    17641 cagcgacgtc tgtatggctg ccgctgaatc cggggaatga tcaatttacg ccaggtcaaa
    17701 acggtccaga ttcatgacct tgttccaggc cgccacaaag tcgtccagga acttctcctg
    17761 cgaatcttta caggcataga cctccgccac ggcccgaagc tgtgagttcg atccgaaaat
    17821 cagatcaacg cgggtgccgg tccatttgag ctcgccggtc ttgcggtcgc gcccttcaaa
    17881 tacatccgca tctttcgaca ccttccactc cgtgcccatg tcgagtaaat tcacgaagaa
    17941 atcgttggtg agcgttcccg gacgcgaggt gaaaactcca tgcggagaac cgccgaaatt
    18001 ggcgcccagc aaacgcaggc cgccgatcag cacggtcatt tccggcgccg ttagcgtgag
    18061 aagctgagcc ttgtccacca gcatctcttc gggcaatacg gtgtatttgg ccttctgata
    18121 gttgcggaat ccgtcggctt ttggttccag cacgccaaac gaagctacat cggtctgctt
    18181 ctgtgaagcg tccatgcgac ccggtgtgaa aggaaccgta attttgtaac cggcattctt
    18241 cgccgcctgc tcgacaccgg cgcagccggc cagaacgatc agatcggcaa gcgatacctt
    18301 cttgtcgcct ttctggtctt tgttgaacgc tttctggatg cgctccagcg ttttgagcac
    18361 gccggccaga cgttccggtt cattcacctc ccaattcttc tgcggagcca ggcggatgcg
    18421 ggcgccgttg gcgccgccgc gcatgtcgga gccgcggaac gtggaggctg acgcccaggc
    18481 cgtcgaaaca agttccgata tggacagacc tgaagctaaa atcttgcttt tcaaggaatt
    18541 ggcatccgct ttgttaatca gcttatggtt gacggcggga atggggtcct gccatatcag
    18601 cacttctttg gggacctccg gaccgagata gcgcgaacgc ggacccatgt cgcgatgggt
    18661 cagcttgaac caggcgcggg caaaagcgtc ggcaaattct ttcggattgg caaggtatcg
    18721 ccgcgcaatc ggcccataaa tcgggtcgaa acgcagcgag agatccgccg tggtcatcat
    18781 cgggcggcgc ttcttggacg ggtcatgagc gtctgcgacc atgtcttcgt cttcgacgtt
    18841 cttggccagc cactgattgg cgccggccgg gctcttgacc agttcccact cgtatttgaa
    18901 cagcactttc agatagccca tatcccaggt cgtcggattc ggtttccatg ccccttcaag
    18961 accgctggag atcgtatcgc cgcccttgcc gcttttataa ctgcttcccc agccaagacc
    19021 ctgcgcttca aggggagccg cttccggttc tggtcccaca tgggtcgcag gaccggcgcc
    19081 gtggcacttg ccgaaggtat ggccgccggc aaccagcgcg acggtttctt catcgttcat
    19141 agccatgcgc gcaaaggttt cgcgcacgtc acggccggag gcgatcggat cgggattgcc
    19201 gtccgggcct tcggggttca catagatcag ccccatctgc acggccgcca gaggattttc
    19261 aagatcgcgc tcgcccgaat atcggctctt gggcttgtcg ctggtcgcca gccattcctc
    19321 ttcgctgccc cagtagatgt cttcttccgg ttcccagacg tccacgcgcc cgccggcaaa
    19381 accgaaggtt ttaaatccca tcgattccaa ggcgcagttg ccggccagaa tcatcaggtc
    19441 ggcccaggag atttttttgc cgtatttctg cttgatcggc cacagaagcc ttcgcgcctt
    19501 gtcgaggttc acgttatcgg gccaggaatt tagcggggcc agtcgctgat tgcctgttcc
    19561 cgcgccgccg cggccgtcac ccatacggta ggtgcctgca ctgtgccagg ccatccggat
    19621 gaaaagtccg ccgtaatggc cccagtcggc cggccaccag tcctgcgaat cggtcatcag
    19681 cgtgatgaga tctttcttca cggcctttag gtctagtttc ttgaattctt tggcgtagtc
    19741 aaatgcttcg cccatggggt tgctcttctt gttctgctga tgcaggatct tgaggttcaa
    19801 ttgattcggc caccagtccc ggttcgattt gccgccgccg gaaatgggtt ttttcatcat
    19861 gcctgttacc gggcatttgc tttcttcggt catctgattt cctcctttct ttcagtgata
    19921 ggggttaaaa aaccttattc cttttgcctt gttgttttct cagtaatctg gaatgattct
    19981 gaattagaat aaaaaccggt catcgtcatt cttttaccgt ttgtttaaaa taccgcggga
    20041 ctgcgcaata agaaaattca gtctgagcgc ttcaggcaat ccctgcaata cccgtgaaac
    20101 tccagtcgtg tttcggttac gacaaaaccc gatttccggg caatgtccct gggttcaagc
    20161 gaggcgggaa tggcgtagtc aaaaatcttc ccgcagcggt tgcagatgag attcacatga
    20221 tccgaaaggt ccacatcata gcggttttcc atccggtcga attccaattg tcggatcaga
    20281 ccgttttcag aaaactcctt aagcgtcgca taaacggtgg acaaactgat acgacgcgct
    20341 ttcttactcg ctgcctcata aatcaaagta gcgccggggt gagcttcgct atgctccacc
    20401 agcgcatcaa taattgccag acgctgcggg gttatcttga gacctttttc ctttaattgg
    20461 ccgatcaggc cgtctttata tatcataagt attctggaat cattccggtt tatggaaaga
    20521 tacaatcatg cctataaatt gtcaagacat attttttcat ggttgaccgg cgggattgac
    20581 aaacgaactt atcaattata tgaaacaaat aataattatt gagagatttc atatgccggt
    20641 ttatttacaa gtaatcctgc tggttgccat ctttatcgcc actgtgatca tttttcttta
    20701 cattggcggc ctggctgtgc ggcgggccag ttttcagatt attgccgaaa tggaagaagc
    20761 cagagccttt aaagagggaa gggcgattaa gattcaggac gagcggaaga acttcttccg
    20821 cgtggggacc aaaaatatcc gcccccgggc gctgaatctg ctcatcgctg atggtctggt
    20881 gattaaaacc aacaacggga aatattatct ggacaaagaa aaactcgccc aagccaaagc
    20941 tcaattaagt tcaaaaacat aaaatgaaca tttcttctta agcttatctt ttatacttcc
    21001 gttgttcata ctgcttaatg ggctcttcga gaggtggaaa aatctcgtga aatcccttgt
    21061 gaagatttgt agcaatcttc gtgatgaaat tggcagtacg gtgggcggtt tctttagttt
    21121 cacgtaatgc ctgttttaag cgcacgctat cgccactgtc agtaatgtac ttttcgtcag
    21181 cttcaccacc cttgtgctca taaacgtgcc tacgatgaaa cattagagta ccaaaagata
    21241 catcctcgct atcgatacta ctgagaacgt ctatgtcaaa tactttgcga aacatctcgg
    21301 aaactgtccc gatgttgtgg aagtgggatt tctctatcca agctttacgg gctggagtta
    21361 atggcactct ggttaggagc tgctttgcgt agtggccggc gaacgagtcg aaaatagcga
    21421 cggtttcttt aacacacgtt tcatatggac caccagcatt gattctatct ctgatttgct
    21481 gaatattttt ttccaactcc tgcaaatcat ttcttgttcc acatgagcta cagtaagcat
    21541 acgttccaag gacgtcatta actttaccac aggcattgca tgtaaatagg ttttgctgac
    21601 gttcttctgc atagtagaaa gctggcttct cgcagtcttt accagccgct tcagccaccg
    21661 aatccatatc gattatgtgt tcgccagact gaccagaagc aagagcgtta ctaagtactt
    21721 cacagtattg cctaacatat cgttgttggg catcagtaag gaaatggtag tactcatcgg
    21781 ccgtaaaggc acaatacgga cagatcttgc caccaagacc tgctcgccaa taaccattac
    21841 atgaggggca ttggtggcca aacatgcctg tgctgtctga tgagataaat actggaaaac
    21901 agtccggcaa cggaggtgga ttccaaggag tgccgattcc acccatctta atgtcatgaa
    21961 cggcaacgcc ttgcggaatg gcataaacag caaacaatgc cgcaggggtt ggtcttgaat
    22021 gagtcaatcc aacattgtag gcgatccggc catcagcgtc gatcttgatg ttaaaattaa
    22081 cctttcctcc tgtatgtgta atttcctcaa gttctgtctt tctattcaat atttcccccc
    22141 acggtttggt gtaaatgtga tcaaactttc tcactccgtc tttgtcggca cacaacaatt
    22201 aatatgcgta cttcatttat tttgatattg tggatgctaa aaaataacac aactttttat
    22261 ttttcaaaag aaaaaatcct atgttttagg gctgttcaaa aatgctcaga tgcaaggcgt
    22321 gcaagaatcg tatcgcgagg cgtatatgaa catacgttga gcggtgcagt ttgcagcaca
    22381 acgccgcaga tggacgtttt ttagcggccc tggagttcgt gttttttcat cataatcaac
    22441 actgccgaaa tcgcgccttc ggtcatgccc ggaatgcgct ccatctggcc aagcgtctgc
    22501 ggggcgattt tggtgagctt taattttaat tcgttggaaa ggccgggcac ggagttgtag
    22561 tcaaagtcag gcgggatttt ctttttttcc tgatctttta atttcttaac ggcgtcgaac
    22621 tgccgcttga tgtagccttc gtatttggtt tcgatttcaa tctgctgttt gacaaaagca
    22681 tccagttccg gttcccagcc gggaaaaatg gaaaggttgt cgtatgacaa ttcatgtcgt
    22741 ttcaaaaggc cgtggagcgt tgtgtgattg ctcaacggcg gagaattgag tgttgcgagt
    22801 ttttcgtttg tttccgcagt cggtttgacc ggcgatgcct tgagctgctc aataccattc
    22861 tctatttttt ttattttatc ctgaagttcc agataatgcg ttttgtcaat taaccccagt
    22921 tcgcaaccct tgcccatcag gcgaatggtt gcattgtctt cgcgtaaaat caggcggtat
    22981 tcggcgcgtg aggtaaacat gcggtaaggt tcatccacgc cgcgggtaac aagatcatcg
    23041 atcatcaccg ccatgtaggc ctcggaacga tccaatag
//
LOCUS       DAONQZ010000036        22605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2554_length_22605_cov_13.783548, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000036 DAONQZ010000000
VERSION     DAONQZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 22605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22605
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_22605_cov_13.783548"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..167)
                     /locus_tag="PLR13_07245"
     tRNA            complement(92..167)
                     /locus_tag="PLR13_07245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Val,seq:tac)
     gene            complement(246..2651)
                     /gene="lon"
                     /locus_tag="PLR13_07250"
     CDS             complement(246..2651)
                     /gene="lon"
                     /locus_tag="PLR13_07250"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQC19171.1"
                     /translation="MFDEKIENEQEKTAIIPLLPLRDVVMFPHMIVPLFVGREKSIAA
                     LESAMKYEKSIFMVAQKNAKDDDPKKEDIYHVGTIGIIIQLLRLPDGTVKVLVEGKTR
                     GMIKEYLPNSDFFLVKVNEVDDIDDGNDVKKQALMRSIKESFELYLKLSKKIHVEMLG
                     TVVAIEDASKLADVVITHLNVKLEDKQKIIEIFDISERLEAIYSLMLSEIEILQVEEK
                     IKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFRNEINELEKRLKQKKMSEEAVKK
                     VKQELKKLQMMTPMSAEATVVRNYIDWILDMPWSEKTENKYTLKESEEILDADHFGLK
                     NVKERIIEYLAVQSLVKKNKGSILCLVGPPGVGKTSIAKSVARATNRKFVRISLGGVH
                     DEAEIRGHRRTYIGAMPGKIIQLLKKAGSNNPVFCLDEVDKLSSDFRGDPSAALLEVL
                     DPEQNFAFNDNYLDLDYDLSEIMFITTANVLQTIPAPLQDRMEVIRIAGYTEPEKLQI
                     AKKYLIAKQVEANGLVMENIEFTHGALLKIIREYTREAGVRNLDREIASICRKVAKEI
                     VNNDKKGRVVIGSKSVGKYLGVPKFHHGEIEEKNKIGLTVGLAWTEVGGELLSIEASI
                     MEGNGKMIMTGKLGDVMQESVQAALSYIRSRADEFGLTKNFYRKIDIHVHVPEGAIPK
                     DGPSAGVAMTTSIASVLLRKKVRADLAMTGEITLRGRILPVGGIKEKVLAAHRGNIKT
                     VIIPKDNQRDLAEVPQNVQKALKIIFAEHIDDVLKLAFVQDEGEMRPAVDANVPMRSQ
                     SVN"
     gene            complement(2670..3932)
                     /gene="clpX"
                     /locus_tag="PLR13_07255"
     CDS             complement(2670..3932)
                     /gene="clpX"
                     /locus_tag="PLR13_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQC19172.1"
                     /translation="MIKKGKNNRMDDGMLFCSFCGKMQSEVRKLVAGPTAYICDECIE
                     LCRYIVEEEDDKLIEKDAQKALPYPKEIKKFLDQYVIGQEKTKKILSVAVYNHYKRLF
                     SRVESDGIEIQKSNVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDV
                     ENIILSLLQNADYDVARASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIME
                     GTMANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNDLESIISKRLGVNAMGFGAEIK
                     SKKEKRIGELLSEVRSEDLLKFGLIPEFIGRLPVIATLDELDEAALIKILVEPKDAII
                     KQYKKLFALENVVLKFTDGALKAVASLSTERKSGARGLRSILENTMLEVMYDIPSQNN
                     IKECVINEDVVLKKEKPILIYESKSESA"
     gene            complement(3933..4532)
                     /locus_tag="PLR13_07260"
     CDS             complement(3933..4532)
                     /locus_tag="PLR13_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQC19173.1"
                     /translation="MVVEQDGRGERAYDIYSRLLKDRIVFLGTAIDDNVANLIVAQML
                     YLEAENPDKEIFFYLNSPGGIVSAGMAIYDTMRYIKSPISTVCMGQAASMAALLLAAG
                     ERKKRFALPHSRILIHQPLGGFQGQATDIDIQAKEILRLKDELNKILADLTQQPLAKI
                     VNDTERDYFMSSEQAKEYGLIDEIIIRKPDASAVRAKEK"
     gene            complement(4544..5869)
                     /gene="tig"
                     /locus_tag="PLR13_07265"
     CDS             complement(4544..5869)
                     /gene="tig"
                     /locus_tag="PLR13_07265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQC19174.1"
                     /translation="MSQVVINVEDLSPVKKKVSLEIPWDDVKNELDAVYQEVGKKAKI
                     KGFRPGKIPRKILETYFKDQAEEETITNIVNKYYWQALEGKGIVPVSRPEINQDGIKD
                     NTNFSFSASFETEQDFEPRHYKGIDVEKEQIKVTDDDVQKRIDEIRQMFATMEEVTDD
                     RPADKGDFVVMDFEGTLNGEKYKELKADDYFLEIGSQKFVPGFEDQLIGMKKGEARDI
                     HVSFPTDYHESRFAGQEVVFHVTVKSIREKKLPENDDSLIKNFEKYESFEDFKNDVRK
                     TLEDKAREMDKVKLQNKITDILVSENELEVPSALVERQIYYMMMETQQKMLSAGIDEN
                     TAMDFNLKMKDKYKPDAEKIVKSFLVLKKIAEKESITATDGDLESYLQELAQHSGRDY
                     ESFQKMYNIEERKDSLMMELVQKKVFDFIEQNANIKVVEKVGLNTEAKS"
     gene            complement(5919..6005)
                     /locus_tag="PLR13_07270"
     tRNA            complement(5919..6005)
                     /locus_tag="PLR13_07270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5969..5971),aa:Leu,seq:tag)
     gene            complement(6279..6355)
                     /locus_tag="PLR13_07275"
     tRNA            complement(6279..6355)
                     /locus_tag="PLR13_07275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6319..6321),aa:Pro,seq:ggg)
     gene            6560..6931
                     /locus_tag="PLR13_07280"
     CDS             6560..6931
                     /locus_tag="PLR13_07280"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQC19175.1"
                     /translation="MKLRDVKEILDAHVVVGEEKLDMDVHTAFGADLMSDVMAFAKAG
                     SLLLTGLTNPQVIKTAASRNIAAIIVVRGKEPTTEAIAMAKDLQIPILTTNYILFETA
                     GILYSKGIVGCLKKVESGFSK"
     gene            7172..7546
                     /locus_tag="PLR13_07285"
     CDS             7172..7546
                     /locus_tag="PLR13_07285"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQC19176.1"
                     /translation="MKLREVKDILNADVIVGEDKLDIEVQTAFGADLMSDVLAFAKAG
                     SMLLTGLTNTQVVRIANVLDIAAIILVRGKKLSTEAISLATDLHIPILTTKYILFETA
                     GRLYAKGIVGCLEKVESGSDRI"
     gene            7533..7976
                     /locus_tag="PLR13_07290"
     CDS             7533..7976
                     /locus_tag="PLR13_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19177.1"
                     /translation="MIESDKNQHVFTFDVEGGNFNAAGTVSTRIKSLLKQLQMPDDVV
                     RRSAIVSYEAEINIVSYAKKGVIRITVSPDQVMIEADDTGQGIPDINLAMQQGYSTAN
                     HLIREMGFGAGMGLFNIKSYSDKFDITSEVDKGTFLRIIIKEHVT"
     gene            7987..8328
                     /locus_tag="PLR13_07295"
     CDS             7987..8328
                     /locus_tag="PLR13_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19178.1"
                     /translation="MDIGIIAEKLNLKFMSASDRKVNNVTGGYTGDLLSDVMANSHGG
                     DVWITRQIHQNIVAVATLKEHAGIILVNACEPAKDTLEKAVQENVPILVSDLSAFELT
                     GRIYNLLRSDK"
     gene            8394..9131
                     /locus_tag="PLR13_07300"
     CDS             8394..9131
                     /locus_tag="PLR13_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQC19179.1"
                     /translation="MMKVFNCDLHIHTCLSPCAELDMHPAALVEKTIDAGLDVIAVCD
                     HNASENVPYVIKAAQGTHLKILPGMEITTSEEVHFLALFDSLSDLIALQHFVDLHLPG
                     KNDEDRFGVQAIVNEHGEVEGLSDKLLIGATDVALNDLLTVVHHHHGLAIASHIDRES
                     FSVLGQLGFIDDSIHFDALEISYATGIRQARERYADLKKYAFITSSDAHYLKDIGRAT
                     TKIMINEPTLSELKMAFSRENGRYVVE"
     gene            9333..9881
                     /locus_tag="PLR13_07305"
     CDS             9333..9881
                     /locus_tag="PLR13_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19180.1"
                     /translation="MLELAAHILDIAENSVRAGAKLVNITIIEDDKKNLLSIEINDDG
                     HGMTEEEIVKALDPFYTTKKVRRVGLGLPLLAEAAKRAGGDLRLKSVKGKGTKLKVTF
                     QLDHLDRQPMGNITTTIRNLIAGNSFVDFLYKHRHNDRRFSLDTRTIRKEIDDLPINH
                     PEILKYIRDVIDDGLEDIQSRA"
     gene            9910..10404
                     /locus_tag="PLR13_07310"
     CDS             9910..10404
                     /locus_tag="PLR13_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQC19181.1"
                     /translation="MKSEDNELLKEFTPEQVDQLNKIIKKHKGKPGSLIPVLEEAQVC
                     LEYLPVSVQKKIAKELNLPLSRVYGVVTFYSFFTMTPRGKHTVRVCLGTACYVRGGKA
                     LTETLQKEFGIHEGETTADRMFTLESVRCLGACGLGPVVVVDEDVHGRVKPSKLKEIL
                     AQYK"
     gene            10432..10815
                     /locus_tag="PLR13_07315"
     CDS             10432..10815
                     /locus_tag="PLR13_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQC19182.1"
                     /translation="MAKLTINDLKKIKEKVHKEMSLRDGERRVKVTVHMGTCGIASGA
                     KEVMDTLLQEIETAGANDVVVTTSGCMGLCSREPLVTVEILNQEPIKYEYMNSNKMRQ
                     VFKRHIQGGEIQTPFVLARGAEVIK"
     gene            10881..13937
                     /gene="nuoF"
                     /locus_tag="PLR13_07320"
     CDS             10881..13937
                     /gene="nuoF"
                     /locus_tag="PLR13_07320"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQC19183.1"
                     /translation="MKVLRAHLLICGGTGCQASGSPAVKKALLEELDKKKLTEEIKVV
                     ETGCNGFCALGPIMVVYPEGVIYISLKPEDMAELVEEHLVKGRIVQRLLYREPTTDQI
                     IPTMQDIPFFAHQELRVLKNRGLIDPEKIEEYIARDGYAGMAKALTEMTPEQIVQEIL
                     DSGLRGRGGAGFPTGLKWKFAAQSKGDVKYVLCNADEGDPGAFMDRSVLEADPHAVLE
                     GMVIAAKAIGSKHGYIYCRAEYPLAIHRLNVAIDQAKKAGLLGKDILGTGFDFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQKPSILNNVETLANIGQIVL
                     RGAKWYASIGTEKSKGTKVFALTGDVANVGLVEVPMGTKLGTIVHDIGGGIPKGKKFK
                     AAQLGGPSGGCIPIQHLNAPVDYEKVAELGAIMGSGGLIVMNEDKCAVDMARFFMDFC
                     QDESCGKCTPCREGTKRMLEILTNITQGKGKEGDIELLEEMAQVIKNAALCGLGQTAP
                     NPVLSTIRYFRNEYEEHIRDHRCRAAVCSAMFKSPCQHTCPIEMDIPAYIALIRAERF
                     DDAYKILLQTNPFPSVCGRVCDHKCQTKCRRGNLDEPVAIKFLKRFITDNAPRPKVDP
                     VLVSRKEKIAVIGAGPAGLTAARDLALRGYKVTVFEELPKPGGMLYWGIPSYRLPRNI
                     LDGEIDDIRALGVDIKLNTRVGKDITFAKLEKEFDYVYLATGAHKSQKMGVKGEDLKN
                     VYGGVEFLRDFNANEEKWLNGKNTLGKKVAVIGGGNSAIDAARIALRLGSEVTILYRR
                     LRQDMPAAEEEIKAAEEEGIKIDYLVAPLNVEGAKGKVTGITCQRMKLGDFDSSGRKK
                     PVPVEGSEFTLKVDAVISAIGQVPDMSFIDAKRGVEINKWDCFAVGKVYKSGTTNPRY
                     FAGGDAVTGPDTAIGAIAAGHQAADDIDAAIRKANGEPAYEKPALEKIDIPLIIDEET
                     VEAPQMAMPELHHETRKTNFVEVELGFKREDAIKEACRCLRCDAAV"
     gene            complement(14048..14968)
                     /locus_tag="PLR13_07325"
     CDS             complement(14048..14968)
                     /locus_tag="PLR13_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19184.1"
                     /translation="MENNRKKKENLGNRQDQNLRHYFETQKELFESWNGNYEKFVTST
                     HNKYKMHYQKEFADFSAFIDYLIKDDEQTVQDTSSSVADDHKKIDSLEAEFDGIFRAW
                     IDQATQERNTDDEDEGKGEWLDYVESQAIATFYSVLINYKEHIENVYHRQLRGKKLGY
                     ARKWMNDDPRVERLIIKYKVCEILNELEKKDSRSAKTKKPFRYLFQLEEPKYQGRYPY
                     ACFITNQDFYNAILQALDIKKITLQKYFKALCQVGAIKKLQGAGRVDIRKNETLYALG
                     YFSGLKGTNYKLNRFLTQKSTNKFLKFKLS"
     gene            15341..15586
                     /locus_tag="PLR13_07330"
     CDS             15341..15586
                     /locus_tag="PLR13_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19185.1"
                     /translation="MPRWTYKTNIIDLDQVIPPDKVQCDLSGDCIVTDLQGGGLPHFK
                     EMLDKEGEGEWELVQCQYHGGKLLCIWKKEIKEAFAS"
     gene            15732..16613
                     /locus_tag="PLR13_07335"
     CDS             15732..16613
                     /locus_tag="PLR13_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HQC19186.1"
                     /translation="MAFNAPAKMVGLVGNAGVYKSKLSVFQFLIRGFMAGAYIAIGGA
                     LCTVCSTGVAPILGPGIGKLIGGAVFPVGLIAIVLTGMELFTGDCMLVPMAAMMKRCT
                     WGSVFRNWFWVYIGNVVGSFFYAYMMAVGPFVDGTADGTLVVNAFGMTAINISVAKIL
                     TYKAVGTAGLWSCFMKAIGCNFLVNIAILLAITADDVISKFFGIWFPIMAFVATGFEH
                     CVANMYFLPTGKFLTDWYPATIEKVGGLLAANGGLSWGDIWFWNIIPVTIGNIVGGFL
                     FIGVAYFLMFRNELPKD"
     gene            16808..17092
                     /locus_tag="PLR13_07340"
     CDS             16808..17092
                     /locus_tag="PLR13_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQC19187.1"
                     /translation="MSKAKLYKIGKVTPAMTGQVVSIKGHVKKISFRWLNRPHFHVED
                     STGRIRVIMFTAPDDNIREGDPVDILGIVIKNIFKRGTPAISAVSVKKNE"
     gene            17119..17268
                     /locus_tag="PLR13_07345"
     CDS             17119..17268
                     /locus_tag="PLR13_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19188.1"
                     /translation="MNSREIIFLIGFLILGITPVFAQQLPGGATELIMLAGMLISTST
                     AFVKS"
     gene            17767..19059
                     /locus_tag="PLR13_07350"
     CDS             17767..19059
                     /locus_tag="PLR13_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQC19189.1"
                     /translation="MANENLESKRWLIAGAAVIIQLCLGTVYAWSVFKNPLMQMHGWE
                     GKSVQYTFMLLMLIIGLAAAFGGTLVDKKGPRFVATIGGILFGIGTLVAGYADQVGNI
                     YLLYLGFGVIAALGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGKIAPV
                     MIKSFQQIDPAGTMIASGVANTWYIWGIIFLILVTGSAQLFKNPPAGWLPKGFTPAAT
                     TVSAADSFTFGEAVKTRQWWMLWAMLCLNVSAGLGLISQHSPLAQDIYKKTYGLTGDL
                     TPEQVAIVAAAGGAVVAYAAIFNGLGRLFWAKISDNIGRKMVFLIMFATQAVLYVLVA
                     KGFVATYWLFVIASCYLLACYGGGFATMPAFAADAFGPAYIGKVYGFMLTAWSTAGII
                     GPYVFEVFKPQALTIAAGLLTLGFVIALIYTPPKGKKA"
     gene            complement(19174..19605)
                     /gene="tnpA"
                     /locus_tag="PLR13_07355"
     CDS             complement(19174..19605)
                     /gene="tnpA"
                     /locus_tag="PLR13_07355"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQC19190.1"
                     /translation="MECQKQAHAVYYTRYHIVVSTKYRRKIFKEGVGEYLQKLILEVS
                     KYHPDIEILEVNTDLDHVHFLASIPPKYSVSEVVGKIKANTGQKMRKRFKFLDKVYWG
                     VEGIWSIGYFVSTVGINESVIRKYIEAQAKEDSGQAKLVME"
     gene            19696..20640
                     /locus_tag="PLR13_07360"
     CDS             19696..20640
                     /locus_tag="PLR13_07360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19191.1"
                     /translation="MINYYINSTEDYHKNELNSLGWELTVCNALDPMDSPCRRLIASQ
                     TSFGENLYRFLEEIIPVDTIRTILEIGGGMGYLMRDFLKFNPRLKATMIDISPYLLGC
                     QKDLLHSFEVDFLLADILTVPPTTLSSFDMAVMNENLGDLPTLVTGSKDDIYHGPAAG
                     VIEKIEAFNIKYNLQLGANQNEPVNIGALEMVEKLCLAGIKYIYLSEHSCEAAVPEKM
                     KNYLFFQTPGIPEMISLKGHREFTIKFSYLQKIAEMLNYTSRRGPFADFLPLDFNDRV
                     KTALRMLTPQSDNQEIIRQFVYDLFKYEYLILIRGDHK"
     gene            20637..20975
                     /locus_tag="PLR13_07365"
     CDS             20637..20975
                     /locus_tag="PLR13_07365"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HQC19192.1"
                     /translation="MKKTVMIIFPALFFLLSVSVSPCHAFRCGNGLVTTGDTKNKVQM
                     TCGKPSAKEKRCLNSWTGKKAKCDKKVEVWYYNCGDNDFVYALIFEGNTLTGENSEGR
                     GHGPSECRGQ"
     gene            21279..>22605
                     /locus_tag="PLR13_07370"
     CDS             21279..>22605
                     /locus_tag="PLR13_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQC19193.1"
                     /translation="MTMDMLIVFGIILLAALLFASEKLRVDLTAIVIMVLLILTGIIS
                     PSEGISGFSNVATITVASMFILSAALQKTGSVNFLGTISSKIFRFNFWVGLIGTMIFV
                     GAASAFINNTPVVAVFIPIMLGVAADNQMSPSRLLMPISFASMFGGVCTLIGTSTNII
                     VSSVAVQHGLPALGMFEFTKMGLVFFAAGLVYMAVVGVRLIPLKKTETDLTKKYQMND
                     YLTDIVLLGNAKSIGKRVADSPLIKELDIDILEVIRNGRRLHVPVSDIVLMENDLLRV
                     RCDIKQLQKLKERMGIKMKSDCELNDENFRCEALSLTEVVVAPNSRLEGKTIKSSYFR
                     NVFHATALALRHRGQLFNTGFTDTILRAGDAILVETRKENYEALKNSNDFVIVSDIET
                     QQYRKSKIIPALLVIAGVIVTASLGIIPIVVGAMIGAILLVLSGCITLEE"
BASE COUNT         5737 a         5447 c         5187 g         6234 t
ORIGIN      
        1 ctccggcgtg acaggccggc gttataacca acttaactac gaccccaaaa aatctttatt
       61 attctatatt cacttttcaa tccgatatca atggtaggcg gaacagggat cgaacctgtg
      121 acatccacgg tgtaagcgtg gcgctctccc ggctgagcta tccgcccgca atgctctcaa
      181 aacgctgcga ggcttctagc aacattgata tttcttgtca agtcataaaa gcttaaaaaa
      241 gctttttaat tgaccgattg ggaacgcatc ggaacgttgg catcgacagc cggtctcatc
      301 tctccctcgt cctgaacgaa agccagtttc aaaacatcat caatatgttc cgcaaagata
      361 attttcagag ctttttgcac gttctgaggg acttccgcca gatcccgctg gttgtccttc
      421 gggataatga ccgttttgat gttgccgcga tgagccgcca gaaccttttc cttgataccg
      481 ccgaccggca gaattctgcc tcgcagggtg atttccccgg tcatggccag atctgcacgc
      541 acttttttgc gcaacaaaac ggaggcgatg gacgtcgtca tggcgacacc ggcggaagga
      601 ccgtctttcg gaatagcacc ctccggcaca tgaacgtgaa tatcaatttt tctatagaaa
      661 ttcttggtca aaccgaattc gtcggcacgc gaccggatgt agctcaacgc cgcctgcacg
      721 gattcctgca tgacatcgcc aagtttgccg gtcataatca tttttccgtt tccttccatg
      781 atggaggctt cgatcgacag aagttctccg ccgacctccg tccaggccag accgaccgtc
      841 agccctattt tattcttttc ttctatttca ccgtgatgga atttgggcac acccagatac
      901 ttaccgaccg atttggaccc gatgaccacc cttccttttt tatcgttatt gacgatttcc
      961 ttggctactt tgcggcagat cgaagcgatt tcccgatcaa gattccggac gccggcttcg
     1021 cgtgtatatt cgcgaatgat tttcaacaag gcgccgtgtg taaattcaat attctccatc
     1081 accaggccgt tggcttccac ctgcttggca atcagatatt ttttcgcgat ctgcagtttt
     1141 tccggttccg tgtatccggc aatgcggatc acttccattc tgtcctgaag cggcgcagga
     1201 atcgtctgca aaacattcgc cgtcgtaata aacatgattt ccgacaggtc gtaatccaga
     1261 tccagatagt tgtcgttgaa cgcaaagttc tgctccggat cgagcacctc cagaagcgca
     1321 gccgacggat cgcctctgaa atcggagctg agtttatcca cctcatccag acaaaagacc
     1381 ggattattgg aaccggcttt tttcaaaagc tggatgattt ttccgggcat cgctccgatg
     1441 taggtccggc ggtgtcctct gatctcggcc tcatcgtgaa ccccacccag ggaaatacgg
     1501 acaaattttc tgttcgtcgc cctggccacc gatttggcaa tggatgtttt tcccacaccc
     1561 ggaggaccga caagacataa aatagatccc ttgtttttct tgaccagaga ttgaacggca
     1621 aggtattcga tgatgcgctc cttaacattc ttcaatccga aatgatcggc atccagaatt
     1681 tcttcggatt ctttcagcgt gtatttgttc tccgtttttt ccgaccaggg catgtcgagt
     1741 atccagtcga tgtaattccg gacaacggtg gcttccgcgg acatgggggt catcatttgc
     1801 agctttttga gttcctgctt gacttttttg accgcctcct cggacatttt cttctgcttc
     1861 aggcgttttt ccaactcgtt gatctcattt cggaaatcgt ctttttcgcc catttccttc
     1921 tggatggcgc gcatctgctc gttgaggtag taatccttct gcgtcttctc catttgtttt
     1981 ttgacgcggc gcttgatctt ttcctcgacc tgcagaattt caatttccga aagcatcagg
     2041 gaataaatcg cttccagtct ctcgctgata tcaaatatct caatgatttt ttgtttgtct
     2101 tcgagtttga cattgagatg agtgatgacc acatcggcaa gctttgatgc atcctcaatc
     2161 gccacaaccg tgcccagcat ttcgacgtga atttttttgc tgagcttgag gtacagttcg
     2221 aaagattctt tgatgctgcg catcagggcc tgtttcttga cgtcgttgcc gtcgtcaata
     2281 tcgtccactt cgttgacttt caccagaaaa aaatcgctgt tcggaaggta ttctttgatc
     2341 atgcctctgg tcttgccttc aaccagcacc ttcaccgtgc cgtccggcag gcgcaaaagc
     2401 tgaataatga tgccgatggt tccgacatga taaatatctt ctttcttcgg atcgtcatcc
     2461 ttggcgttct tctgcgcgac catgaaaata ctcttttcgt atttcatcgc cgactccagt
     2521 gcggcaatgg atttctcacg gcccacaaac agaggaacaa tcatatgagg aaacatgaca
     2581 acatctctca acggcaaaag agggatgatg gccgtttttt cctgttcgtt ttctatcttt
     2641 tcatcaaaca tactttccct ttactgcatt taagcggatt ccgatttgga ttcgtaaatt
     2701 aaaattggtt tttctttttt cagaacgacg tcttcattga ttacacattc tttgatatta
     2761 ttttgcgatg gaatgtcata catcacttcc aacatggtat tttccagaat cgaacgcaac
     2821 ccgcgggcgc cggattttct ctccgtggat aagctggcca ccgccttcaa ggcgccgtcg
     2881 gtaaatttca aaaccacgtt ttccagcgcg aataatttct tgtactgctt gatgatggca
     2941 tctttcggct cgaccagaat cttgatcagc gcggcttcat ccagttcatc cagagtggca
     3001 atgacgggca accggccgat gaattccgga atcagaccga atttcagcaa atcttccgat
     3061 cggacttcac tcaacagttc accgatgcgt ttttctttct tgcttttaat ttccgcgccg
     3121 aaccccatgg cgttgacgcc cagcctcttg gagatgatgc tttccagatc attgaaagcg
     3181 ccgccgcaga taaacaaaat attggtcgtg tccacctgga taaattcctg ctgaggatgt
     3241 ttgcgaccgc ctttgggcgg aatattggcc atggtccctt ccatgatctt caaaagggcc
     3301 tgctgcactc cttcgccgga tacgtcgcgt gtgatggacg gatttcccga cttcttcgcg
     3361 attttatcga tctcgtcaat gtatacgatt cccttcgacg cacgggcgac atcgtaatcc
     3421 gcattttgga gcagacttaa aataatattt tccacatcct cgccgacata acccgcttcg
     3481 gtcagggtag ttgcgtcggc aatggtgaac gggacattca gaaacttggc cagagtcttg
     3541 gccagcaagg ttttaccgga gccggtggga ccgatcagca gcacattgct tttctgaatt
     3601 tctataccgt cggattcgac gcgcgaaaaa agtcgtttgt aatggttata gacggcaacc
     3661 gacaaaatct tttttgtttt ttcctgaccg atgacgtact gatccagaaa ttttttaatc
     3721 tctttcggat acggcaaggc tttttgagcg tctttttcaa tcagcttatc gtcttcttct
     3781 tcaacaatat aacggcagag ttcaatgcac tcgtcgcaaa tataggctgt cggccccgct
     3841 acgagcttgc gtacctcgct ttgcattttc ccgcaaaagg aacaaaaaag cattccgtca
     3901 tccattctgt tattcttgcc tttttttatc acttatttct ccttggccct gacggctgaa
     3961 gcatccggct ttctgatgat gatttcatca attaaaccgt attctttggc ctgttcactg
     4021 ctcataaaat aatcccgctc cgtatcattg actattttag ccagcggctg ttgtgtcaaa
     4081 tcggccagga ttttgttcaa ctcgtccttt aatcttaata tttcttttgc ctgaatgtca
     4141 atatccgttg cctgtccctg aaaaccgccc agaggctgat ggattaaaat ccgcgaatgc
     4201 ggcagtgcga atctcttttt tctttctccc gccgccagaa ggagggctgc catactggcc
     4261 gcctgtccca tgcataccgt agaaatcggt gatttgatgt agcgcatcgt atcataaatg
     4321 gccatcccgg cactgacaat gcctcccggt gaattcagat agaagaaaat ttccttgtcc
     4381 ggattttcgg cttccagata aagcatttga gcgacaatta gattggccac attgtcgtcg
     4441 atcgccgtac cgagaaatac gatacggtct ttcaacaaac gggaatagat atcgtatgcc
     4501 cgttcgcccc ggccatcctg ttcaaccacc ataggaatca acgtcacgat ttcgcctccg
     4561 tattcagtcc gactttttcc acgaccttaa tatttgcatt ctgttcaata aagtcaaata
     4621 cctttttttg aaccaattcc atcatcaggg aatcttttct ttcctcaata ttatacattt
     4681 tctggaacga ctcgtaatct ctcccgctgt gttgcgccag ttcctgaaga tagctttcca
     4741 gatcgccgtc cgtcgcggta atggattctt tttccgcaat cttctttaac accaggaaag
     4801 atttaacgat tttttccgcg tcgggtttgt atttgtcctt cattttcagg ttgaagtcca
     4861 tggcggtatt ctcatcgata ccggcagaga gcattttttg ctgcgtttcc atcatcatgt
     4921 aatagatctg ccgttcaacc agcgcggagg gaacttccaa ttcattttcg ctgaccagaa
     4981 tgtctgttat tttgttttgc aacttaacct tgtccatctc gcgggctttg tcttccaggg
     5041 ttttccggac atcattcttg aaatcttcaa aagattcata tttttcaaaa tttttaatca
     5101 aactgtcgtc gttttcgggc aatttttttt ctctgatgct cttaacggta acgtgaaata
     5161 ccacttcctg gccggcaaat ctgctttcat gatagtccgt cggaaacgat acgtgaatgt
     5221 ctcttgcttc accttttttc attccgatca gctgatcttc gaaaccgggg acaaatttct
     5281 gggatccgat ttccagaaaa taatcgtctg ctttcagttc cttgtatttt tcgccgttca
     5341 aagttccttc gaaatccatc acaacgaaat cacccttatc cgccgggcgg tcatcggtca
     5401 cttcctccat ggtggcaaac atctgcctta tttcgtcgat tcttttttgc acgtcatcat
     5461 cggtcacttt aatctgttct ttctccacgt ctatgccttt gtaatgacgg ggctcaaaat
     5521 cctgttccgt ttcgaaagac gctgaaaaag aaaagttagt attatccttg ataccgtcct
     5581 gattgatttc cggtctggaa acgggcacaa tcccctttcc ttccaaagcc tgccagtagt
     5641 atttattgac aatattggtg atggtctctt cttccgcctg atctttaaaa tatgtttcca
     5701 gaattttacg gggaattttg ccggggcgaa agcctttaat tttagccttt ttacccactt
     5761 cctgataaac ggcatccagt tcatttttaa cgtcatccca gggtatttcc agtgacacct
     5821 tctttttgac cgggcttaaa tcctcaacat ttataacaac ctgactcact atcccttctc
     5881 ctttatcgtt gaattatgct ttttcgtttt ttaaaatctg gtgcgagaga ggggagttga
     5941 acccctattc cgcatcgcgg gctggatcct aagtccagtg cgtctgccag ttccgccact
     6001 ctcgcatgga cattcttata tattcactta gtttaagatt gtcaacgcag attattttgc
     6061 aaaatcacaa gaaatttcat ctatttaaga ttttacggcc ccattgttta tttgattgtc
     6121 tgcttttatg caagaaaaaa taaattttct tttccggacg ctcatcgtac agaaaccaga
     6181 tgaatcgcga aaggatgcct tgtcgaatgc ccatttcatt caaccggtat ttaaacacaa
     6241 agggcaaacc ggataggtgg tttgcccctt aaattaattg gtcggggcga gtggatttga
     6301 accaccggcc ctctgaaccc cattcagata cgctaccaga ctgcgccacg ccccgatgaa
     6361 atacaaattg acgtgcgtca ttaccattac ggccaacccc tgtcaagggt aaataaattg
     6421 acgcttatcg caacgatatt tttatgtcgt gagatggatt attccccttc attcataaaa
     6481 aaatcttaat aattaattga cactttttgt gtttttatgc ctataataaa aaagttaaca
     6541 tgacacaaag gagttttttt tgaaactaag agacgttaaa gaaatattgg acgcccatgt
     6601 cgttgtcggt gaagaaaaac tggacatgga tgtgcatacg gctttcggcg ccgatttaat
     6661 gagtgatgtt atggcctttg ccaaagcagg cagccttttg ctgaccggat tgaccaatcc
     6721 ccaggtcatc aaaaccgctg ccagtcgaaa tatcgccgcc atcatcgttg tccggggaaa
     6781 agagcccaca accgaagcca tcgcaatggc caaagatctg caaattccga ttttaaccac
     6841 aaactatatt ttatttgaga cggccggcat tctgtacagt aaaggaattg tgggctgtct
     6901 gaaaaaagtt gaaagcggtt tttccaaata aaacggtttc ccggtttttc tcagaacatt
     6961 caaaatatcg aagatccatt ttttaagtta tttttaaaag aatcacttga aaacacgtcg
     7021 gtgcgtgtcg aaatctctct ttttctgttc gttttttttg attttaacaa aatgatttca
     7081 ttgacagtaa agtctcttct atgataaaag atttttaatt taattttaga tatttaaaac
     7141 aaaaataacc tttttcagca aggagaaaat attgaagctt cgtgaagtca aggacatact
     7201 caatgccgat gtgattgttg gagaggacaa gctggatatc gaggtgcaaa cagcttttgg
     7261 agcggatctg atgagtgacg ttctcgcttt cgccaaagcg ggaagtatgc tgcttacggg
     7321 actgaccaat acacaggttg tccgaatcgc caatgtcctg gatattgcgg caatcatttt
     7381 agtacgcggc aagaaacttt ccaccgaagc gatttctctg gctacggatc ttcatattcc
     7441 gattttaacg acaaaatata ttttatttga aacggccggt cgtctttacg cgaaaggtat
     7501 agtcggctgc ctggagaaag ttgaatccgg cagtgatcga atctgacaaa aaccagcacg
     7561 tattcacgtt tgacgttgaa ggaggtaatt tcaatgccgc gggaactgtt tccacccgca
     7621 ttaaatccct tctgaagcaa ttacaaatgc ctgatgatgt ggtcaggcgg tcagccattg
     7681 tttcctatga agcggaaatc aacattgtct cctacgcaaa aaaaggggta atccggataa
     7741 cggtcagtcc cgatcaggta atgatcgaag ccgacgatac gggtcaggga atacccgata
     7801 tcaacctcgc catgcaacag ggctattcca cggccaatca tctgatcagg gaaatgggtt
     7861 tcggcgccgg catgggattg ttcaacatca agtcgtactc ggacaagttt gacatcacat
     7921 cggaagtgga caagggaaca tttttaagaa ttatcattaa agaacacgtt acataaagga
     7981 tggattatgg acatcggaat catcgcagaa aaattaaacc tcaaattcat gtcggcgtcc
     8041 gacagaaaag tgaataacgt tacaggaggg tacacggggg atctgttaag cgatgttatg
     8101 gccaattcac acggaggaga cgtctggatc accagacaga ttcatcagaa tatcgttgcc
     8161 gtggccacgc tgaaagagca tgccggcatc attctagtca acgcctgcga gccggccaag
     8221 gacacgctgg aaaaagccgt tcaggagaac gtgccgatac tggtatcaga cctttcagca
     8281 tttgaattaa ccggcagaat ctataacttg ctgcgttcgg ataagtaaga gcgaattctt
     8341 tatgtctccc cggcacaaag ctgcctgata ttgtattgtc ccgtccggaa aacatgatga
     8401 aagtttttaa ttgcgacctt cacattcaca cctgcctgtc gccctgcgca gaactggaca
     8461 tgcatcccgc cgctttagtg gaaaaaacca ttgacgccgg tctggatgtg attgccgtct
     8521 gcgatcacaa cgcttcagaa aatgttccct atgtgatcaa ggcggcacaa ggcacgcact
     8581 tgaaaattct tccgggaatg gaaataacga ccagcgagga agtgcacttt cttgccctgt
     8641 tcgattccct ttccgatttg atcgccctgc agcatttcgt cgatctgcat ctcccgggaa
     8701 aaaatgatga ggaccggttt ggcgttcagg ccatcgtcaa cgaacacggc gaggttgaag
     8761 gactcagcga taaattattg atcggagcta cggatgttgc gttgaatgat ttgctcaccg
     8821 tcgttcatca tcatcacgga ctggcgattg cctcgcacat cgacagggaa agtttcagcg
     8881 tcctgggaca gctcggtttt atcgacgaca gcattcattt tgacgctctg gaaatatcgt
     8941 atgcaaccgg gattcggcag gcgcgggaaa gatacgcgga tttgaaaaaa tacgctttca
     9001 tcacctcatc cgacgcccat tatttgaagg acatcggcag agccacaacg aaaatcatga
     9061 taaacgaacc gacgctgtcc gaattgaaaa tggctttttc aagggaaaac ggccgttatg
     9121 ttgtggaata gtccatgcga cagcatcctc tgtatgcatc gtcgcatcgt tgaccggacc
     9181 cgaccttttt ccgctgatgc ggccgagctg ttcatgccgc gcttttagac ttcatgcctt
     9241 acgcaatcct gaaggcattt ttacagatct cgtcgggttt tgcgatttgc ttcggaaacc
     9301 cggagatcca tgaaacggga gatttataac tcatgctgga acttgccgca cacattctgg
     9361 atattgccga aaactctgtt cgggccggcg ccaaactggt gaacatcacc atcatcgaag
     9421 acgacaagaa aaatctgctt tccattgaaa tcaatgatga cggacacggc atgaccgaag
     9481 aggaaattgt caaggcgctt gacccttttt acaccacaaa aaaagtcaga cgcgtaggac
     9541 tgggccttcc gcttcttgcg gaagccgcaa aaagagcggg tggcgacctt cgtctgaaat
     9601 cggtcaaagg caaaggaaca aaactcaaag tcacctttca gctcgatcac ctggacaggc
     9661 aaccgatggg gaatatcact acgacaatac gcaatcttat tgccggaaat tcttttgttg
     9721 actttcttta taaacatagg cataatgacc gccggttctc actggatacg agaacaatcc
     9781 gcaaggaaat cgatgattta ccaataaatc atccggaaat tttaaagtat attagggatg
     9841 ttatcgatga cggtttagag gatattcaat cccgcgcgta acagaccaat aagattgagg
     9901 aggaaattga tgaagtcgga agataatgaa ctcttgaaag aattcactcc agaacaggtg
     9961 gatcagctaa acaaaattat taagaagcac aaagggaaac cggggagttt aattccggtt
    10021 ttggaagaag cgcaggtatg tctggaatat ctgcccgttt ccgtgcagaa gaaaattgcc
    10081 aaagaactca acctgccttt gagccgcgtt tacggtgtcg ttacatttta ttcattcttc
    10141 accatgacgc caaggggtaa acataccgtc cgagtctgtc tgggaacggc gtgctatgtg
    10201 cgcggaggaa aggcactgac cgaaaccctg cagaaggaat tcggaattca cgaaggcgaa
    10261 acgacggcag acagaatgtt tacgctggag tccgttcgct gcctcggcgc ctgcggtctc
    10321 ggaccggttg tcgttgtgga tgaagatgtc catggacgcg tcaagccatc caagttaaaa
    10381 gaaatactag cccaatacaa ataaaataaa aaaagccggg aggaaacgtc aatggctaaa
    10441 ttgaccataa atgacttaaa aaaaataaag gaaaaagttc ataaggaaat gtctctccga
    10501 gacggcgaac gtcgtgtaaa agttaccgtg catatgggaa cctgcggcat cgcctcggga
    10561 gccaaggaag tgatggacac gctgcttcag gaaattgaaa cagccggagc caatgatgtc
    10621 gtcgtcacca catccgggtg catgggatta tgcagccgcg aaccgctggt aacggtagaa
    10681 atcctgaatc aggaaccgat caaatacgaa tatatgaatt cgaacaaaat gagacaggtt
    10741 ttcaagagac atattcaggg cggagaaatt cagacacctt ttgtgctggc ccggggagct
    10801 gaagtaatca aataatattt tccatcgata atgcttgttg ccacaaagca cgatgatttc
    10861 ttaaggagga tgtaggctaa atgaaagttt tacgcgcaca tttactgatt tgcggaggca
    10921 ccggctgtca agcttccggg agccctgcgg taaaaaaagc tttattggaa gagcttgata
    10981 aaaagaaatt aacggaagag attaaagttg ttgaaacggg atgcaacggc ttttgcgccc
    11041 tcggccccat tatggtcgtt tatcctgaag gcgtcattta tatatccctg aaacccgagg
    11101 atatggcgga attggtggag gaacatctgg tcaaaggacg aatcgtccag agacttcttt
    11161 atcgtgaacc gacaacggat cagattattc ccacgatgca ggacatcccc ttcttcgccc
    11221 atcaggaact cagagtcctc aagaaccgcg gtctgattga tccggaaaaa atcgaggaat
    11281 acatcgcccg cgatggatac gccggcatgg ccaaggcgtt aaccgaaatg acccccgaac
    11341 agatcgtgca ggaaattctg gattcgggat tgagaggacg cggaggcgcc gggttcccga
    11401 caggtctgaa atggaaattt gcagcacaat ccaagggcga cgttaaatac gttctttgca
    11461 atgccgacga gggagacccc ggagcattca tggacagaag cgtcctggaa gccgatccgc
    11521 atgcggttct ggaaggcatg gtgatcgccg ccaaagcgat cggatcaaaa cacggataca
    11581 tttactgccg cgccgagtat ccgctggcca ttcaccgttt gaatgtggcc atcgatcagg
    11641 ctaagaaagc cggccttttg ggcaaagata ttctgggcac gggatttgat ttcgatctgg
    11701 agatttatca gggcgccggc gcatttgtct gcggcgaaga aacggctctg atgacgtcta
    11761 tcgaaggcaa aagaggtatg ccgcgcccca gacctccctt ccctgccgtt gccggtctgt
    11821 ggcagaaacc aagtattttg aacaacgtgg aaacgctggc caatatcgga cagatcgtcc
    11881 tgcgcggtgc caaatggtac gcctctatcg gaacggaaaa gagcaaggga acaaaagtct
    11941 ttgcgctgac cggagatgtg gcgaatgtcg gtctggtgga agttcccatg ggaacaaaat
    12001 tgggaactat cgttcacgat atcggcggcg gcattcccaa aggcaagaag tttaaagccg
    12061 ctcagctggg cggaccgtcc ggcggctgta ttcccattca gcatttgaac gcgcctgtcg
    12121 attacgagaa agttgctgaa ctaggcgcca tcatgggctc cggcggtctg attgtcatga
    12181 atgaagacaa gtgcgctgtg gacatggcca gatttttcat ggatttctgt caggatgaat
    12241 cctgcggcaa atgtacaccc tgccgcgagg gaaccaaacg catgctggaa atcctaacca
    12301 atattaccca gggcaaggga aaggaaggcg acatcgagct tctggaagaa atggcccagg
    12361 ttatcaaaaa cgccgctttg tgcggtctgg gacagacggc tcccaatccc gtattgagca
    12421 ccattcgtta tttccgcaat gaatatgagg agcatatccg cgatcatcgc tgccgcgcag
    12481 ccgtatgttc ggcgatgttc aaatcaccct gtcagcatac ctgtccgatc gaaatggata
    12541 tccctgctta catcgctctg atcagggctg aaagatttga cgatgcctac aaaattcttc
    12601 ttcagaccaa ccccttcccc tccgtttgtg gaagggtctg cgatcataaa tgccagacaa
    12661 aatgccgtcg cggcaatctg gatgaaccgg tcgccattaa attcctgaag cgattcatta
    12721 ccgacaacgc ccccagaccc aaagtcgacc ccgttttggt cagcaggaaa gaaaaaattg
    12781 ccgttatcgg cgccggtcca gccggtttaa cggccgcgcg cgatctggct ttacgcggct
    12841 acaaggtgac cgtttttgaa gaactgccca aaccgggtgg aatgctttac tggggcattc
    12901 cgtcctatcg tctgccgcgc aacattctgg acggtgaaat cgacgatatc cgtgcgctcg
    12961 gcgtggacat caaactcaac acccgggtcg gcaaggatat taccttcgcc aaactcgaaa
    13021 aagaattcga ttacgtctat ctggccaccg gtgctcacaa gagccagaag atgggtgtca
    13081 aaggcgaaga tctgaaaaat gtttacggcg gcgttgaatt tctgagagat tttaatgcca
    13141 acgaagaaaa atggctcaac ggcaaaaata cgctgggcaa gaaagtcgcc gtgatcggcg
    13201 gcggtaattc cgccatcgat gcagcgcgta tcgcgcttcg ccttggctct gaagtgacca
    13261 ttctctatcg tcgcttgaga caggatatgc ccgcggccga ggaagaaatt aaggcggctg
    13321 aagaagaagg catcaagatt gattatctgg tcgctccttt gaacgtcgaa ggtgcaaaag
    13381 gtaaagtgac aggcatcacc tgtcaacgca tgaaactggg cgattttgat tccagcggtc
    13441 gcaagaaacc cgttccggtt gaaggatccg aattcaccct gaaggtggat gcggtgatct
    13501 cggccatcgg ccaggtcccg gatatgagtt tcatcgatgc caagcgaggc gtggaaatca
    13561 ataaatggga ttgctttgct gtcggcaagg tttacaaatc cggcacaacc aacccccgtt
    13621 atttcgccgg cggtgacgct gtaaccggtc cggataccgc gatcggagcc attgctgcgg
    13681 gacatcaggc tgccgacgat atcgatgccg ccattcgcaa agcgaacggt gaaccggctt
    13741 atgaaaaacc ggcacttgaa aaaatcgata tcccgctcat tatcgatgaa gaaaccgttg
    13801 aagctccgca gatggcaatg cctgaactgc atcacgaaac ccgtaagacg aatttcgtgg
    13861 aagtggagct aggcttcaaa cgcgaagacg ctattaaaga ggcgtgccgc tgcctgcgct
    13921 gcgacgcggc tgtttaattt actctaaagg taatgcaaaa aaagaggctt gcccttgtcg
    13981 ggtaggcctc ttttctttgg tcgatttcta aaaacaaaga ctgtggaaga agcatccgaa
    14041 ttgttgatta agacaatttg aattttaaga atttgtttgt gcttttctgc gttaagaatc
    14101 tgttcaattt atagtttgtt cctttcaagc ctgaaaaata ccccagtgca tacaacgttt
    14161 cattcttcct gatatcgact ctgccggcac cctgcaattt cttaatggcc cccacctgac
    14221 acaacgcctt aaaatatttc tggagggtga ttttttttat atctaacgcc tgaagaatgg
    14281 cgttgtagaa atcctggttt gtgataaaac aggcataggg atatctgcct tgatatttcg
    14341 gttcctccag ttgaaacaaa tagcggaaag gttttttggt tttggcgctt cggctatctt
    14401 tcttttcaag ctcattaaga atttcacaaa ccttgtattt tataatcaac cgctccaccc
    14461 tcggatcgtc attcatccat ttccgggcat atccgagctt ctttccccgt aattgccgat
    14521 gatagacgtt ctcgatatgt tctttgtaat tgattaatac actgtaaaac gtcgcaatcg
    14581 cctgggactc cacgtaatca agccattcgc ccttcccttc gtcctcgtca tccgtatttc
    14641 tttcctgcgt cgcctggtcg atccatgccc ggaatatgcc gtcaaattca gcctccagag
    14701 aatctatttt tttatgatcg tccgcaacgc ttgatgacgt gtcttgcacc gtttgttcat
    14761 catccttaat caggtaatcg ataaatgcgg aaaaatcggc aaactccttt tgatagtgca
    14821 ttttatattt attgtgtgtc gaggtgacaa atttttcata atttccattc cagctctcga
    14881 ataattcttt ttgagtttca aaataatgcc ggagattttg gtcctgccgg tttcccagat
    14941 tttctttttt tttgcggtta ttttccatgc ctgcttataa acctccaaac cagtatccgt
    15001 ataaaatatc gacaacgttt tttttcaatt gtgttgaccg gtttaaaaaa aatattcctc
    15061 ttgcacaatc atctataagg accttccatc gggatgtcaa ttcttttgag gtaactataa
    15121 gttcttgaac ttattcatga cttacggttc ttcatatata tcacaaatta ttaccttttt
    15181 tttgacaaat ttaacaattg agcgtataag gaatcgttaa atttctggca acaactgccc
    15241 tgagctctgc gtgtataagc aatgattcat tcatgcatac atgacaaaga ccggcagtaa
    15301 aataacattg tttcaaaata ttcagaaagg agatgagatc atgccacgat ggacgtacaa
    15361 aacaaacatt attgatctgg atcaggtgat cccgcccgac aaagtccaat gcgacctgtc
    15421 aggagactgc attgtcacgg atcttcaggg aggaggcctt ccgcatttca aggaaatgct
    15481 cgacaaggaa ggcgaaggcg aatgggagct tgtgcagtgc cagtatcacg gaggcaaact
    15541 gctgtgcatc tggaaaaagg aaattaaaga agcctttgcc agttaatcaa tccggattgg
    15601 tctgtcatca gcccttccgt tgagcgcgca taacgataga ggctgatcag ggatgaaggt
    15661 gaaaaaaatc cagtcatgcg gctttgatgc gtcgtacgct ggttatttta atgaatggag
    15721 gtagtagtgt catggctttt aatgcaccag ccaaaatggt aggtcttgtc ggcaatgccg
    15781 gcgtttacaa atcaaaactt tcagtatttc aatttcttat ccggggcttc atggcaggag
    15841 cctacatcgc gatcggcggt gcgctgtgca ccgtgtgttc gaccggtgta gcgcccatcc
    15901 ttggccccgg catcggcaaa ttaatcgggg gtgcggtgtt ccccgtcgga ttgattgcca
    15961 tcgttctcac cgggatggaa ctttttaccg gagactgtat gctcgtcccc atggccgcaa
    16021 tgatgaaacg gtgtacatgg ggttcggttt tcagaaactg gttctgggtc tatatcggaa
    16081 acgttgtcgg ttcttttttc tatgcctata tgatggcggt tggcccgttt gtggacggca
    16141 ccgcggacgg cacgctggtc gtcaacgcct tcggaatgac ggcgatcaat atttccgtgg
    16201 ccaagattct gacgtacaag gccgtcggga ccgccggcct ctggtcctgc ttcatgaagg
    16261 ccatcggttg taatttcctg gtcaacatcg caattttgct ggccattacg gcggatgacg
    16321 tgatcagcaa gtttttcgga atctggttcc cgatcatggc cttcgttgcc accggatttg
    16381 aacactgtgt cgccaatatg tatttccttc ccacaggcaa gttcctgacg gactggtatc
    16441 cggccaccat cgaaaaagtc ggcggccttt tggcagccaa cggcggttta agctgggggg
    16501 acatctggtt ctggaacatc attccggtta ccatcggcaa tattgtcggc ggatttcttt
    16561 tcatcggtgt ggcctatttt ctgatgttca gaaatgaact tccgaaagac tagaggaatc
    16621 gcaattgtca ctaccggcat ggataacttt ttttatctgc gcggcggccg tattcattca
    16681 ctatatgcag gtgaggaata cccggttttt gctctttgcc attcccgctt tgctcgctct
    16741 tctcattatt ccgatgcttt tggcatggat gagtcggaga acgttaaata aagcggctca
    16801 agaaaacgtg tccaaggcaa agttgtacaa aatcggcaaa gtgactccgg ccatgacggg
    16861 acaggttgtc agcatcaaag gccatgtcaa aaaaatcagc tttcgatggc tcaacaggcc
    16921 gcattttcat gttgaagact caacaggccg gatccgggtg attatgttta ccgcacccga
    16981 tgacaatatc cgagagggag accccgtaga catactggga attgtaatca aaaatatttt
    17041 caaacgcggc accccggcca tatcggccgt cagcgttaag aagaacgaat agataacaga
    17101 cactttcagg aggtcgtcat gaattcaaga gaaatcatct ttctaatagg atttttaatt
    17161 ctgggcataa cgccggtttt cgcgcaacag cttcccggcg gagcaacgga attgatcatg
    17221 ctcgcaggca tgctgatttc cacatccaca gcttttgtta aatcgtaaca tccgggcaga
    17281 gcgaagcggc ggccggtcgt cttcgctctg cccctccatg atctttccat aaaacaggaa
    17341 attgcttacc ggattttggc taatttattt ttgttagtgt tttcatcaat cgacacatcc
    17401 gtacatgcgc cgatctcatt gcatctttcc atcggccggg tttgaagccg gaatcgtggc
    17461 ttcttatctc ttctaaaaag aacggcttgc ttttttgctc aaaaagaaag tttcgagcct
    17521 tatgaatcaa tataattttc taatattatt cataacataa aatcctttcc atccctccga
    17581 gaaaacacga ctttgtgttt gacaaaataa tgtattgcag ctataagcgg cagtcaaact
    17641 gctgactgat aagttttcga agtccaaaga gaaatttgtc ccattggaag cccgttcagt
    17701 cgtattacag gcagctcaat ataagaagaa gttataataa atttactaaa gtgaggtaag
    17761 gaatttatgg caaatgaaaa tttagaaagt aagaggtggt tgattgccgg cgcggctgtt
    17821 atcattcagt tgtgtctcgg caccgtgtac gcatggtcgg ttttcaagaa tcctttgatg
    17881 cagatgcatg gatgggaagg caaatctgtt cagtacacgt ttatgctgtt gatgctgatc
    17941 atcggattag cggctgcttt cggcggtacc ttggttgata aaaagggacc gcgttttgtc
    18001 gccaccatcg gcggcatttt attcggtatc ggcaccctgg tggccgggta cgcagatcag
    18061 gttgggaaca tttatttgct ttacctgggt ttcggcgtta tcgccgctct gggtaacggt
    18121 ttcggttacg ttacgccgat tgccacgctc attcgctggt tcccggacaa acgcggtctg
    18181 gtaaccggtc ttgccgtgat gggtttcggc gccggtgctt tcttcatggg gaaaattgct
    18241 cccgtgatga tcaaatcctt ccagcagatc gaccccgcag gaaccatgat cgcttccggc
    18301 gttgccaaca cctggtatat ctggggtatc atattcctga ttcttgttac aggttccgct
    18361 cagttattta aaaatcctcc ggccggttgg ctgcccaaag gattcacacc tgccgccaca
    18421 accgtgtccg cggcggattc ctttaccttc ggagaagctg tgaaaacccg tcagtggtgg
    18481 atgctctggg ccatgctgtg cctcaacgtt tccgcagggc ttggcttgat ttcccagcac
    18541 tctcctctgg cacaggatat ttacaaaaaa acttatgggt taacgggaga cctgacgcct
    18601 gaacaagttg cgatcgttgc cgccgccggt ggcgctgttg tcgcttacgc cgccatcttc
    18661 aacgggctgg gccgcttgtt ctgggcaaaa atttccgata atatcggccg caaaatggta
    18721 tttcttatta tgtttgccac ccaggccgtt ttgtatgtgc tcgttgccaa aggttttgtt
    18781 gcaacatact ggctttttgt catcgcttcc tgttatcttt tggcctgtta cggcggcgga
    18841 ttcgccacca tgcctgcttt tgccgctgac gctttcggtc ccgcttacat cggtaaagtc
    18901 tacgggttta tgttgacggc atggagcact gccggtatca tcggccctta tgtttttgaa
    18961 gtattcaaac ctcaggcgct cacgattgcc gccggcttat tgaccctcgg ttttgtcatc
    19021 gcactgattt acacccctcc gaagggcaaa aaagcctgat acgtcttatc tcaaaagccc
    19081 cgtccgtatt caacggacgg ggctttttca caccttgccg cacatttgcg tctatcatta
    19141 ttttaattct gttgacattt aacagggtat tgtttattcc attacaagct tcgcttgtcc
    19201 gctgtcttct ttcgcctggg cctcaatgta tttccgaatt acgctctcat ttatcccgac
    19261 cgttgaaaca aagtagccta tcgaccaaat accttccact ccccaataga ctttgtcgag
    19321 aaatttaaat cttttcctca tcttctgtcc ggtgttggct ttgattttac ccacaacctc
    19381 actcactgag tatttcggtg gtattgacgc caaaaaatgt acgtgatcca aatccgtgtt
    19441 cacttccaat atttctatgt ctggatgata tttgctcact tccaatatga gcttttgcaa
    19501 atattcgcca acaccttcct tgaatatttt tcggcggtat ttggtcgaaa ccactatatg
    19561 atatctcgta taatataccg catgggcttg tttttgacat tccattcata tagtatatac
    19621 gaagacagcg gatacttcca tccacaggct cacgcctgtg gtattacgaa tgtggaataa
    19681 aattttaatt ttcgcatgat aaactattat attaattcta cggaagatta tcacaaaaat
    19741 gaactcaact cgcttgggtg ggaattaact gtctgcaatg cgcttgatcc tatggacagt
    19801 ccctgccgaa ggcttattgc ctcacagaca tcattcgggg aaaatttata tcgttttctg
    19861 gaagaaatca tacccgtcga cacaatccgt acgattctgg aaataggcgg cggcatgggt
    19921 tatctcatgc gtgatttctt gaaattcaat ccgcgcctga aggcaacaat gattgatatc
    19981 tctccctacc tgctgggttg tcagaaagac ctgttacact cttttgaagt cgattttctt
    20041 ctggccgata tcctcacggt gccaccgacg acattatcct ctttcgacat ggccgtgatg
    20101 aatgaaaatc tgggcgatct tccgacactg gtgaccggat caaaagacga catctaccat
    20161 ggccctgccg ccggagttat cgaaaaaata gaagccttca atataaaata taacctgcag
    20221 ctgggagcca atcaaaacga acccgtcaac atcggcgccc tggaaatggt ggaaaaactc
    20281 tgtctggccg ggatcaaata tatctatctg agcgagcaca gctgtgaagc cgccgttccg
    20341 gagaaaatga aaaattatct cttttttcaa acacccggca ttccggaaat gatttccctg
    20401 aaaggacacc gcgaattcac catcaaattt tcctatttgc agaaaattgc ggaaatgcta
    20461 aattacacct cgcggcgcgg accgttcgcc gattttctcc ctctggattt caatgacagg
    20521 gtaaaaacgg cattgcgaat gttgacgccg caaagcgaca accaggaaat tatcaggcag
    20581 tttgtttatg atctttttaa atatgagtat ttaattctta ttcgaggaga ccacaaatga
    20641 agaagaccgt catgattatt tttccggcat tatttttctt gctgtctgtt tccgtcagtc
    20701 cctgccacgc ttttcggtgc ggaaacggac tcgtcacgac cggagacacc aaaaacaagg
    20761 ttcagatgac ctgcggaaag ccctccgcca aggaaaaacg atgtctgaat agctggaccg
    20821 gcaaaaaggc caaatgcgat aaaaaagtcg aggtatggta ctacaactgc ggcgacaacg
    20881 acttcgttta cgctctgatt ttcgaaggca atacattaac cggagaaaac tctgaaggcc
    20941 ggggccatgg accttcagaa tgccggggac agtgaacata gcactggtgg ctccgatcct
    21001 gatagccgga atttaaaaga gctgagcgtt gtttcgcttc aagatgtttt tcgaatgtac
    21061 gccgctgtta agagaaatcc gccgggaaag cgccatgatc gtaacgcttt tataatgaat
    21121 aatcattgac ttgccttcat gatttcttct atactgtctt tgtggaagca agtccttctc
    21181 cctgatctcc gaaggataac gcatgtacaa atgcagccgg cgtggcgtca ggtcccgcat
    21241 acttttgatc cttaaggata tctcaaaagg ataaacctat gacaatggac atgttgatcg
    21301 tattcgggat catcctgctg gccgccctgc tgtttgcctc ggaaaaactt cgcgtggatc
    21361 ttacggcaat cgtcatcatg gttcttctca ttctaaccgg catcatttca ccctccgaag
    21421 gcatatccgg cttcagcaat gtggccacca tcacggttgc ctccatgttc attctcagtg
    21481 cggcccttca gaaaaccggt tcggtcaatt ttctcggaac gatttcttcc aaaatattca
    21541 gatttaattt ctgggtggga ctgatcggca cgatgatctt tgtaggcgcc gcatcggcgt
    21601 tcatcaataa tacgccggtt gtagcggttt ttattcccat tatgctggga gttgcggcag
    21661 acaatcaaat gagcccttca cggcttctga tgccgatttc tttcgcgtcc atgttcggcg
    21721 gcgtgtgcac cctgatcggc acatcgacaa atattattgt gagttccgtc gccgttcagc
    21781 atggattacc agccctgggc atgtttgaat tcaccaagat gggactggtt ttttttgccg
    21841 ccggtctggt ctatatggcg gttgtggggg tccggctgat cccgttgaag aaaacggaaa
    21901 cggacctgac gaaaaaatat cagatgaatg attatctgac ggatatcgtg cttctcggca
    21961 acgccaaatc catcggcaaa cgcgtcgccg attcaccgct gatcaaagag ctggacatcg
    22021 acatccttga agtcattcga aacggacggc gtcttcacgt ccccgtgtcc gatatcgttc
    22081 tgatggaaaa cgatcttttg cgtgtgcgtt gcgatatcaa gcagttgcaa aagctcaaag
    22141 agcggatggg cattaaaatg aaatcggact gtgaactcaa cgacgaaaat ttccgctgcg
    22201 aagccctcag cctgactgaa gttgtggtgg ctccgaattc acgtctcgag gggaagacca
    22261 tcaaatcctc gtatttccgt aatgtctttc acgcaacggc actggcgctg aggcacaggg
    22321 gacaactgtt caacaccgga tttaccgata cgattctcag agcgggcgat gccattctgg
    22381 tggaaacgcg caaagaaaat tatgaggcgc tgaaaaacag caacgatttc gtcatcgtct
    22441 cggatattga gacgcagcaa taccgtaaaa gcaaaatcat tccagcactg ctcgtcattg
    22501 ccggcgtgat tgtaacggcc agcctgggca ttattcccat tgtggtcggg gcgatgattg
    22561 gcgctatcct gctggttctg tccggctgca tcacccttga ggaag
//
LOCUS       DAONQZ010000037        22339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2604_length_22339_cov_19.137812, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000037 DAONQZ010000000
VERSION     DAONQZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 22339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22339
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_22339_cov_19.137812"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..697)
                     /locus_tag="PLR13_07375"
     CDS             complement(233..697)
                     /locus_tag="PLR13_07375"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQC19194.1"
                     /translation="MLNEDYKDILHALSDENVRFLLVGAYALAAHGYPRATMDIDIWV
                     MPSPENADAVLRALQRFGASLQNLTKEDLQKDGTIFQIGVAPRRIDIITAASGLQFEP
                     AYQHSIQLNIEEMEVRIPSIDDLILNKKATGRTKDLADIEILESLKNSEKRL"
     gene            complement(1026..1583)
                     /locus_tag="PLR13_07380"
     CDS             complement(1026..1583)
                     /locus_tag="PLR13_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQC19195.1"
                     /translation="MELQEAILKRRSVRKFTDDVVTDEELKQIFEAVRWSPSWANTQT
                     WEFIVVRDKALMEKVAGTYSEKNPATKCSLSASALIVACAKTAISGCYGGKQVTSISN
                     WEFFDLGIAVQTLCLKAHEMGLGTVVVGLMNHEACEKILAVPEGHKVYAVIPIGRPAT
                     EPKAGPPRKSLSEMVFLNNLNKPMF"
     gene            complement(1587..2309)
                     /locus_tag="PLR13_07385"
     CDS             complement(1587..2309)
                     /locus_tag="PLR13_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQC19196.1"
                     /translation="MFGYLFILLFLYLPVYVFAKNRATALQNLNHIHLKIFFTLTKRL
                     APKTKYNIPDNLREIHSSVVVCNHLSYLDPILLVSLFKKQKTIVKHTFFKVPIFGWFL
                     KNYGYISSGSDTMMGAAMINNLEDIKQHLSSGGVLFVFPEGTRSRDGSLSPFNKGVFT
                     IARYCNAPLKLILIRGTNKLFQPGHFLFHTQEHNEIELRLIGSLNPDYKSKDFSIAAL
                     ADEARKIFEEKIDHLKSDNKGD"
     gene            complement(2386..3624)
                     /locus_tag="PLR13_07390"
     CDS             complement(2386..3624)
                     /locus_tag="PLR13_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQC19197.1"
                     /translation="MERRVVITGMSAITPIGHGKDQIVQSLLNGTSGIKPLREEEFLS
                     SFIHSKVYGTVDYPVEYDFKRNHRKTMGPVAYYACQVAREVLEQSGLTEDFITSGRLG
                     VAFGSIHGSPTIQRNIYKIFFGAQDKIAVKEIGAVDYLKSMVHTTAVNITKMFGITGR
                     VISSGTACTTSSQSIGYGYESVKFGMQDAMLCGGADEYDTTTVAVFDNLLACSTAFNN
                     IPSKTPRPFDKDRDGLVVGEGAGAVMLEEYEHARKRGATILGEVIGFACNNNGGDMIL
                     PNLTGITNTLKLGVETAKINPEEVDLISAHATATKMGDIIEAQAINAVFGHNPHVIGL
                     KSYTGHTMAACGAIETIMALYMMQEGFIAPTLNLENIDERCAMLKHVQKLTDHPVKTA
                     AIQNFAFGGVNTMLMIKKFS"
     gene            complement(3632..3901)
                     /locus_tag="PLR13_07395"
     CDS             complement(3632..3901)
                     /locus_tag="PLR13_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQC19198.1"
                     /translation="MTRQEIEREVKNVFQREFEIDNPDMDADLRETYEFDSIDAIELL
                     LAIETFLNTEITQEEKKQAISIRTINQICDYIVEIAKKRNLFTPA"
     gene            complement(3973..5451)
                     /locus_tag="PLR13_07400"
     CDS             complement(3973..5451)
                     /locus_tag="PLR13_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQC19199.1"
                     /translation="MRNSVDGKNIVIIGSGIGGLSTGIILSLMNFHVTVVEKNPLPGG
                     LMRSYRRGGIDCPVGIHYVGALGAEEPLGKMFAALGIPVDELFVPMAGEGIVDRYIFD
                     DLTFDLPDGIDTLENSLRDTFLQEKPALDVLMKNLRDISRQMTDPSFLFAQGDPFQNM
                     GYYQPMGELLDSLNVSPALRAVLSVPCNLIGVPLNDCPVIFHHMVLASYLFSSWRLKE
                     CGRKMTDVFIRRFEDLGGQLVLHDGVKKILFEKGKVSGVILESARTLFADAVVAAIHP
                     KVMLQLLDENVLRDSHRRRILGLKETESVIAVQVSVDAAKHPEMHHNIYRLHRDENGV
                     IQEGVFYQVQRTTSAGLHLLSIITKSLFGDWSRWENTVTGQRGSEYEEHKMNLAKGLL
                     KKAEDVFGDLNDARILDVYTPLTLRDYVSAPEGACYGIMRSSRQLLKAISLNNLPIPG
                     LCLAGQNAMAPGVMGCLFGAFNAVRQITGAERFAREINENMQ"
     gene            5908..11289
                     /locus_tag="PLR13_07405"
     CDS             5908..11289
                     /locus_tag="PLR13_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC19200.1"
                     /translation="MEKISDYVLEKKIHKTKNAVTYRGYKEKDFQSVIIKLLNTLHST
                     PSEIAQFRQEYELIQRLDIGSVVKTIDIIHYKGGLALILEDFDGIPVKDYFRPNEKIN
                     IASFLALSANIAATLAALHARGVIHRNIKPQSLLINPETEELKITDFGISALLTHEHD
                     EVYNPDFIRDTLTYMSPEQTGRMNRSVDYRTDLYSLGITLYEIVTGLLPFKSQDPMEL
                     IHAHIAMMPRAPVKLDAGVPAMVSDIIMRLLAKNPEARYQNGFGLQADFQECLKQWEE
                     KKRIEPFELGRQDISNRFIVPQKLFGREKEISELISRFEEVANGDKRLSVMVVAGAPG
                     IGKSAMINEIHKPIVARRGYFISGKYEQFRRDKPYSAIIQAFQVLVKQILSESEQRIN
                     LWKKNLLSTLGDNGRVITDVIPEVEWIIGKQPDLPVLGPEETRNRFKFIFEKFMSVFP
                     QQEHPVALFLDDLQWADMASLQLMKNIMLSDIHHLFILFSYRDNEVNETHPFSELLSE
                     VKKQNIRIDKITLDPLKEKEVTELIVNFLRCSEERGFGLAGLVQEKAGGNPFFVNQFL
                     HTLYNEKMIVHKGAQGWQWDVGEISRMKVADNLIQMMAGKIEKLPDNAQDVLKICACI
                     GNRFDLETLATVRKTSVDEALADLTEAIKEGMVSRLGDFYVFHHDRIHEAAYSLVTDA
                     EKSALHYKIGRILMGNLDEKELPGKLFYIVDQLNLGVQMIQNEEEREKLARFNLEAGK
                     KAKESAAYVPAYQYLKNGMNLLEENPWIKQYPLTLALYTETTEAAYLMGDYDKMNALA
                     EITLQHAKTNQEKTRIYTSRINACIAREDYRGAIDVAMPILNTIAPRFGIRLPKKANK
                     LHIALEIFRIMLKLAGKRSEDLINLPKTSDLEILAAGKILASVSHAAFYTDPNLLALI
                     ILKTFRLIFKYGLAPEHAFAFAGFGIVQAAGLWDFKSAIAFGKMGLKLVEKLDARDQA
                     ARTVFVYNALVRHWKYPMKDSIEPMIEGYKVGLETGDLGFATFNLYFTEVHYIFTGRE
                     LSDLEKYMERNNRIIAGMKQGHTLTLQSMTWQAILNLLGRCDNSLKLTGKAIDAEKLL
                     PSWEETENLAALSVFWFVKLILYSLFSEYKMALKASDEFRKYMDSQQGVLINKYAVLL
                     DSVARLKVYKNASLPEKIRHRVLLKINELKIRAWAKAGPMNCSYMYTAIKALYAWVIR
                     GNLAKAEKLVELAVRQCREYGDFVVEGIASEIMAGEYRSIGENEKAKRFLDRTGICYS
                     TWGATGLLNKIRKMYPELVSSEADVSLNVEATTAEKTKDDLSVALDLSTVMQVSQVIS
                     SEIMLERLLEKIMHMSVTSAGAQRGYLMLESEGKLLIEASEDIDNDETNVMQAMPLHD
                     CADICQAIVNYVHHSGKDVILGHAAKEGAFTDDSYINNKQCKSVLCIPIIHKGKISGI
                     LYMENNLSEDAFTPGRLDILRFISAQAAISIENAKLFELATTLENIEDGYWEIDLAGN
                     YTFLNNAVCQIHGYSKEELIGMNNRQYTDEETAKKVYKAFNNVFKTGQPLKGLDWQII
                     RKDGTKRYVEISILLQKDSSGKPIGFRGVVRDITERKNMEEKLQQTLESLSKAYDSTL
                     QVMVSAVEVRDPYTALHQVRSADVARNIAIEMGLPQEKIDAIRMAGAIHDIGKLSIPP
                     EILTKPEKLTSVEYALIKEHSRSGYEVLKNVESPWPLAKIVHQHHERMDGSGYPRNLK
                     GEEILLEARILAVADVISAMVSHRQHRPSLSIEVALEEIEKNKGTLYDNSVADACLRL
                     FREKGYKLN"
     gene            11762..13318
                     /locus_tag="PLR13_07410"
     CDS             11762..13318
                     /locus_tag="PLR13_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQC19201.1"
                     /translation="MVTGTVLVPKSLYNYGLTARPIISYAVGTHGLVQSWAPSIQLEN
                     GTDYENSNIAAALNRGYAVLVTDNPGYTNNDVPSYMVGKAQGQAVLDIVTAALQIPNI
                     SLKSYAKVGIWGYSQGGQSAAWAGQLQSTYAPGIKLAGVAAGGVPAELFSVGRYLDGN
                     NGSSFLLSTCIGLNAQYPNDILLNSRVNTDGMDAIGRGLSMGVFEALFAFMNTPLSDF
                     TKYGETSDQIFAIPEIAEVVNGQELGKTKINAPVFLYHGTGDEFIPLEQALNLKEKYC
                     SLGVNTSYMVYPGEHITTQFQAAPQVLDWLKDRFAGKSAASTCRTSNPRPASTANPVD
                     GDFLFSLDGWKLDGMIKLKTLMTKVSLPEGSTFSAETNMTNNTITGGMDIPEFSYYIY
                     AFGLMPLQVKLKIVPAGTMTGTASLDKNGILHINGNVKADIYLKKVGELGIGIPFSLK
                     TKTPVDFPIVFDGPVSSLGDGSLTFTGTTTFPDMVENGIIINALFTGLMSGPGQEFTF
                     TVTPPAPVAW"
     gene            13445..13963
                     /locus_tag="PLR13_07415"
     CDS             13445..13963
                     /locus_tag="PLR13_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19202.1"
                     /translation="MTRSDMKKNKSKTKIILAALIVGMAVIAGIVYWHSGNDGDTGAA
                     VDATQPVALNMSEQDIVDAEKNASIAAVSGETVAGTVTERPDYITEIEWQVLQSVTRQ
                     NPDIKVNDLVNKMLFAKKKQAWLSAEKDSAERKLLAKQLLDMIPGQVAMEAIDGATAQ
                     TMEAELHADLLR"
     gene            complement(14293..18636)
                     /locus_tag="PLR13_07420"
     CDS             complement(14293..18636)
                     /locus_tag="PLR13_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQC19203.1"
                     /translation="MKSHHLHHKGKSFSGKQGETVMVAGCDLGKSTAKFVMLNVCDSD
                     TPILEDFRCIVHHGNPLNAFREWYREKHIASCAALAATGLHSEELVAPVISGLPEDAC
                     ISAALQLQQDLPEQINLVSLGARGYSVMTRDRQGHVGYVENDKCSSGTGETIVKIAGR
                     FGLGLEEADQLAFGAAVSVPISARCSVFAKSEMTHFGNQGKPTDQLMKGYFESVARNT
                     AALLSRVRIEGPVYLMGGGARLASFVKAFREIAGGTVFVLDEPQLFEALGAAVLAADQ
                     YREDKLPKLPVDPEQLIIPRRTRFRIQSPAQQWASNVTYQSAPDVTEDPAVTPAVLGL
                     DLGSTGSKAVLTSIRTGNQLTDVYDRTRGNPVDASLRLLDEILRQTKPDVRAIALTGS
                     GREAAATVLRAVFPELAERIVVLNEIVAHATAAIRCDDQKGKSLSIVEIGGQDAKFIQ
                     IAGGQITESDMNKACSAGTGSFLEEQAVFYDIHDVKEFTRLAQKAKRPPDLGQMCTVF
                     IADAAAEAHNEGFSIEDLFGGFQYSVIHNYINRVMGGRTFGDRIFFQGKPASGPSLAW
                     TLAAVTGREVIVPHNPGAMGAWGIGLCAIRELGEEKLFAAGTFDLNLALNARVVSRIE
                     FRCADSKCHNFCHIDKTTVAVGAAEKTVLSGGACPKYEISSIASKKIPKEAPNALEER
                     MALLENFREAGTGDREVGVPYVGVLNGYLPWMVTFLSEIGFRVKVLRSDSKSLAGGEE
                     RCFSFDSCAPAKIAHAVADADVDTLVFPKFLNLHDRDGHGGRTCPMEQGMADMADQAL
                     KARGRNIRFVKPLLNLRDGLLNRRLIGRLRGLARELGVDDRHVKTAARRAAKAQQDYE
                     HALAEIGRRTLAYGRGQGIPVVAVIGSLHVIYDRAVNASIPDLLRQNGVLSLPMDCYP
                     IPDDIPELPRIVWEDANRALRVALSARNCGEVFPLFLSSFGCGPASFIEHFFRFLMEG
                     YPHTALESDGHGGFAGFVTRIQAFLHTVRQHDGRPVPAESSRLRLFDTLERPPLASQK
                     DSKFVVFTMGDQTAPIMAAVYRSWGYDAIPAIPTSAVSLAAGRRDCSGKECLAYQLIW
                     GSFRKYLDDNPPEKRTVLLQISGRGACRTCNFSMKDQISIENMGLSDRVSLRHVAAEL
                     ECPGLVSRFYTGTLVWNLLNQLAAYHRPYESEPGEVDRIYDDYCSDLIRILEKPSGRG
                     FSGIISKMGSVGRIVSGSIEQNQGSALFEQLVRRASTQFAAMRKCRTDQNSLRTIFLT
                     GDIYLRLDDFGSDAIIRRLNARGIRVIVEPLWVLAEYLVEERSAELFSLPQGTFMNAM
                     AKVLLPKSRKHFYEMAQEQNPWLPTFDVPEMLKKAKPIIDRHPRGEAPITVGSVIHHW
                     DKAVSDGIVIVGPWGCGPALIAESLLKHQRQIPTLFVYNDGSPLDERKLNGFVFRLRR
                     QEPKTATETGR"
     gene            complement(18853..19521)
                     /locus_tag="PLR13_07425"
     CDS             complement(18853..19521)
                     /locus_tag="PLR13_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19204.1"
                     /translation="MRRHLLWIVIFFLSSCQTLPIINAPSSPRKEKSFVCPDPFLKRN
                     YRLVHAIEARLDGRVQSQIIGITLALPDTRSVSCAIITVEGMSLFEAEANPHGINVKR
                     ALPPFDSKDFANNMMEDIKLIFLAPEGVLQNRGYLADGSKACRYRQENGGWIDVIENE
                     SGETQIKRYSSSEVLKRDVRFSGTHKQLYQRIGLQADAKFDYFLLMNLIEDQPIKKQA
                     IKRN"
     gene            complement(19518..20123)
                     /locus_tag="PLR13_07430"
     CDS             complement(19518..20123)
                     /locus_tag="PLR13_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQC19205.1"
                     /translation="MILLTGFCFLYGNVCHAEEKGFEKLRKESVKIKTLQADFTQKKS
                     MKILSRPLVSQGYFYFAMPDSLRWEYIKPLKSVVLSHNQQARRYMHSDGKWVEDKTNG
                     VQAMRIVLNEIAGWMNGRFDQNPSFKATVIGKENTCIILTPVGKKMKGMIERIEINLA
                     PQARVVQSVKILEGPDNITQINFSNVKMNHAIDPTIFKDVR"
     gene            complement(20153..20881)
                     /gene="fabG"
                     /locus_tag="PLR13_07435"
     CDS             complement(20153..20881)
                     /gene="fabG"
                     /locus_tag="PLR13_07435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQC19206.1"
                     /translation="MNDKKTAIITGASRGIGRATAVQLARAGYFVIINFKTNETAAQE
                     TLNMVKSEGGDGTIIPFDVADSEQTRNAVKTITEQYKNIQALINNAGITADGLFMLLG
                     EEEWDSVINTSLNGFYHMTKPILREMIRKKRGSIVSVSSLSALMPNRGQANYAAAKAG
                     IIAASRSLASEVARFGVRVNVVAPGLIETDMIKDAPVDQIKQIIPMARLGKPQEVASV
                     IRFLCSDDASYVTGQVIGINGGIC"
     gene            complement(20905..21333)
                     /locus_tag="PLR13_07440"
     CDS             complement(20905..21333)
                     /locus_tag="PLR13_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19207.1"
                     /translation="MLDIDIDSLIPHRKKIKIINHVIDIQENSAVSAAMVGPDWPLSD
                     GKTVDALVLIEGIAQTAAIVEGYKRKQRGAGAVKGWLVGIKNAAFYEEKIPLNTHLVI
                     LLENKDAFDNYGVVEGTVKTGEKILATAVLQAVRLNDNDQ"
     gene            21470..>22339
                     /locus_tag="PLR13_07445"
     CDS             21470..>22339
                     /locus_tag="PLR13_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC19208.1"
                     /translation="MMTQAKKDPFVGLYKKMLELSPSVCRKDFTFGGYSYGDVLALAA
                     GLKKILDRRSGKKNICLCSANKAVTAACALAALSGSCKLVLPYSFSAHAMKEMSEATG
                     FNYAIADHPEEMPEGVEIITPVPAAPEILRPESLIHPDETFLRLFTGGSTGKPNVWSK
                     SPRNLLSEAFYLKERFCLSTKDLFIATVPPYHIYGLLFTVFLPFVARAAVLPDIYTFP
                     QEIISTINKHKASVLVCVPIHYRVLKVDNLSVPSLRAAYSSSGMLDRSDSLYFYRKTG
                     LGITEVYGSTETGG"
BASE COUNT         5789 a         5368 c         5500 g         5682 t
ORIGIN      
        1 gtaattctca gcgtcacata cagactgacg atcatgatca gaactttgcg gataaaagag
       61 gctttcataa cattcaactt ccccttctta ttttgaaaat attttcatca tcgctgggta
      121 ttttgcggat gatcgcttta aattaaatat taacacatcg gcttatcgag attcatagtg
      181 gcttcaatcg aaggacgact aaaccagtat cagaattatt tgtgcatttg tttcaaagac
      241 gtttttcgga attttttaaa gattccaaga tttcaatatc ggcaagatct tttgttcttc
      301 ctgttgcttt tttattgagg ataaggtcgt cgatcgaagg aatacggact tccatttcct
      361 ctatattcag ctgaattgag tgttgataag caggttcgaa ctggagcccc gaggccgcgg
      421 tgataatgtc aatccgtctt ggggcaactc cgatttgaaa aattgttccg tccttctgaa
      481 ggtcttcttt ggttaaattc tgcaatgaag cgccaaaacg ttgcaaagcc cgcagaaccg
      541 catcagcgtt ttccggagat ggcatgaccc agatatcgat atccatcgtg gcccggggat
      601 aaccatgagc cgcaagtgca tacgctccga caagaaggaa cctaacgttc tcgtcggata
      661 aggcgtgtaa aatgtctttg tagtcttcgt tcagcatgat gctttatact cagggaggat
      721 atttcacgtg tcaacggcca aacaagcaga atccgctctt ccggcgtgcc ggggacataa
      781 ttgtcgtcct gatgatccgt cattcggtgt atggcggttt gctttttatt cataaattta
      841 aattaccatt ttttataagt aaaatcaacc ggatattccc tgtggccggt tccgaaaatt
      901 tcgaaaatct ggggacactt tgctattttt tatatcttca tttatgaata acgcagccgg
      961 aagctgaagt tacacgcttt ttggaaaggc agggagatag ctccctgccg gtgggttgga
     1021 atgatttaga acatcggctt attgaggtta ttcaaaaata ccatttcgga aagggatttg
     1081 cgcggcggtc ctgccttggg ttcggtggcc ggtcggccga tcggaatgac ggcataaact
     1141 ttgtgacctt cgggcacggc cagaattttt tcacaggctt cgtggttcat taaacccaca
     1201 accacggttc caagtcccat ttcatgggct ttcaggcaaa gcgtctgaac ggcgatgccc
     1261 agatcgaaaa attcccagtt ggaaattgaa gtgacctgtt tcccgccgta gcaaccggaa
     1321 atggctgttt tcgcgcaggc gacaattaga gcggacgcac ttagagaaca ttttgtggcc
     1381 gggttttttt cggaataggt gccggcgact ttttccatca gcgctttgtc gcggacgacg
     1441 atgaattccc aagtctgagt attggcccag gagggcgacc agcgtacggc ctcaaagatt
     1501 tgtttgagtt cttcatcggt gacaacatcg tcggtaaatt ttctgacgct tctgcgtttt
     1561 aaaatggctt cctgaagttc cataacctag tcccctttgt tatctgattt taaatggtct
     1621 attttttctt cgaatatttt tctggcctcg tcagccaggg cagcgatgga aaaatctttg
     1681 cttttgtaat ccggattgag cgaaccgatt aaccggagtt caatttcatt atgttcttga
     1741 gtgtggaaaa ggaaatgacc gggttgaaac agtttgttgg ttccccggat taaaatgagt
     1801 ttcaatggcg cgttgcaata acgggcaatc gtaaagacgc ctttgttgaa cggcgaaaga
     1861 ctgccgtcgc ggctccgggt gccctccgga aaaacaaata aaacgccgcc cgacgacaga
     1921 tgctgtttga tatcttccag gttgttgatc atggccgcgc ccatcatggt atcggatccg
     1981 gaggaaatat acccgtagtt tttcaagaac cagccgaaga tgggaacttt gaaaaaagta
     2041 tgcttgacga tggttttctg ttttttgaaa agggacacca gcaggatggg atcgagatag
     2101 gacaaatggt tgcagacaac aaccgatgaa tgaatttccc gcaaattatc gggaatatta
     2161 tattttgttt taggcgcaag ccgtttcgtc aacgtgaaga atatttttaa atgaatatgg
     2221 tttaaattct gaagagcagt agcgcggttt tttgcaaaaa cgtaaacggg cagatataaa
     2281 aacagtaaaa taaaaaggta tccgaacatg aaatagcacc acaaaatcag tgttacggat
     2341 aaattaactg caaaatgaaa aatacgggac agaaacatct tcaactcagg aaaacttttt
     2401 aatcatcagc atagtattga cgccgccaaa agcgaaattt tgaatggccg ctgtttttac
     2461 cggatgatcg gttaattttt gtacatgttt taacatggca cagcgttcat cgatattttc
     2521 cagattcaac gtcggcgcaa taaagccttc ctgcatcatg tagagggcca tgatggtttc
     2581 aatggcgccg caggcggcca tggtgtgacc ggtgtagctt ttcaacccga tgacgtgagg
     2641 attatgaccg aagacggcgt tgatggcctg cgcttcaata atgtcgccca tcttggtggc
     2701 cgtggcgtgc gcgctgatca agtcgacttc ctccggattg attttggcgg tttccacacc
     2761 cagcttaagc gtgttggtga tgccggtgag attcggcaaa atcatgtcgc ctccgttgtt
     2821 gttgcaggca aaaccgatga cttctcccag aatagtcgcg ccgcgctttc tggcatgttc
     2881 atattcctcc agcatgaccg ctccggcgcc ttcgcccacg accaggccgt cgcgatcttt
     2941 gtcaaacgga cggggcgtct tcgacggtat gttgttgaag gctgtggaac aggccagcag
     3001 gttatcaaaa acagcaacgg tggtggtgtc gtattcgtcg gcgccgccgc agagcatcgc
     3061 atcctgcatg ccgaatttga cggattcgta accatacccg atcgactgcg aactggtcgt
     3121 gcacgccgtt cccgacgata tcacccggcc ggtgatgccg aacattttgg tgatattgac
     3181 ggcggtggta tgcaccatgg atttcagata atcgaccgcg ccgatttcct tgaccgcgat
     3241 tttatcctga gcgccgaaaa agattttgta aatatttctc tgaatggtcg gcgaaccatg
     3301 aatggaaccg aaggctacgc ccagtcttcc ggatgtgata aaatcctcgg tcagaccgga
     3361 ctgttccaga acttccctgg cgacctgaca ggcgtaatag gccaccggcc ccatggtctt
     3421 gcggtgattc cgtttaaaat cgtattccac cggataatcc accgttccgt aaactttgga
     3481 atgaataaag gagctgagaa attcttcttc gcgcagcggt ttgatgccgg aggtgccgtt
     3541 taacaggctt tgaacgatct gatctttccc gtgaccgatc ggcgtaatgg ctgacatccc
     3601 tgtgataaca actctgcgtt ccataatgat tttatgccgg cgtgaaaaga tttctttttt
     3661 ttgcgatttc gacaatgtag tcgcaaatct gattgatcgt gcggatactg atggcctgtt
     3721 ttttttcttc ctgagtgatt tccgtattca gaaaggtttc aatagccagc agcaattcga
     3781 tggcgtcaat actgtcgaat tcataggttt cgcgcaaatc agcatccata tccggattgt
     3841 cgatttcaaa ttcccgctga aaaacgtttt ttacttcgcg ctcaatttcc tgacgtgtca
     3901 tagataggac tttaactttc tgaatgtgat gttgtgacct ttaacaaata tcgtatggct
     3961 tttcaatgaa attcattgca tattttcgtt gatttcccgg gcaaatcgtt ccgcacccgt
     4021 gatttgccgg accgcgttga aggcgccgaa aagacagccc atcacgcccg gcgccatggc
     4081 gttttgtcct gccagacaca aaccgggaat cggcagatta ttcagcgaaa tggccttcaa
     4141 taactgacgc gacgaacgca tgatcccgta gcaggctcct tccggagcac tgacataatc
     4201 ccggagggtg agaggcgtgt atacatccag aatccgggca tcgttcaaat cgccgaagac
     4261 gtcttcagcc tttttaagca ggcctttggc gaggttcatt ttgtgctctt catactcgct
     4321 gccgcgttgg cctgtcacgg tattttccca tcgactccaa tcaccgaaga gggatttggt
     4381 aataatcgaa agcagatgaa ggcctgcgga ggtcgtgcgt tgcacttgat aaaatacgcc
     4441 ttcctgaatc acaccgtttt catcacggtg gagcctgtaa atgttatgat gcatttcagg
     4501 atgcttggcg gcgtcaacag agacctgaac cgcgataacg ctttccgttt ccttaagtcc
     4561 cagaatgcgc cggcgatgag aatcgcgcag aacattttcg tccagcaatt gaagcatgac
     4621 cttcgggtgt atagcggcaa ccaccgcatc ggcgaaaagc gttctggccg attccaaaat
     4681 cactccgctt actttaccct tttcaaacaa gattttcttg acgccgtcgt gcaggacaag
     4741 ttgccccccg aggtcttcaa agcggcggat aaagacatcc gtcattttac ggccgcattc
     4801 cttcagtcgc cacgaagaaa aaagataact ggctagcacc atgtgatgga aaatgaccgg
     4861 acaatcgttg agcgggacgc cgataagatt acagggaacc gataaaacgg cacgcaaggc
     4921 cggtgatacg ttcaaagaat ccagtaattc tcccatcggc tggtaatagc ccatattctg
     4981 aaagggatca ccctgagcga acaagaacga gggatccgtc atttgccggg aaatgtcgcg
     5041 cagatttttc ataaggacat ccagcgccgg tttttcctga aggaacgtat ctctcagact
     5101 gttttccagc gtgtcgattc cgtcgggtaa atcgaaggtt aaatcgtcga aaatatagcg
     5161 gtcgacgatg ccttctccag ccatgggaac aaatagctcg tcaaccggga ttcccaaggc
     5221 cgcaaacatt ttaccgaggg gttcttccgc ccccaatgcg ccgacgtagt gaatcccgac
     5281 agggcagtct attccgccgc gccggtaaga ccgcatcaat ccgccgggca gcggattttt
     5341 ttccacgacc gtgacgtgaa aattcatcaa agataaaata atcccggtac tcaatccgcc
     5401 gataccggaa ccgatgatca cgatgttctt gccatcgacg ctatttctca tctgcttgcc
     5461 cctttttgtt tttctgtatg catttgtttt ctataagtca accggaaatg atcctttcgt
     5521 ctgccggggg ataaaaacaa attgacatcg gggaaagtta tttctatact ttaaccattc
     5581 cagaaaaaaa gcgcggcaca tatccgttgg ggggatttct tatatcaata agaaaaggat
     5641 tgctgcgttc gcggtcgcca tcgttcttgg cattgttgcg gcaccggtta tcaaaaacag
     5701 gttgacgggc gagcacgccc aacaggatta tgtctttgat aaaaattcca gaatcccatt
     5761 aaaagatatc aaaacccatt attagtatgt tgatttcgag aaggaaaaag ccgcggagta
     5821 tttgaatgat gatgtgacca atccctatac gataaaattt ttaatgtttc ttaacagccg
     5881 ttgaaaatat agaagagaaa tacatttatg gaaaaaataa gcgattatgt tctggaaaaa
     5941 aagatacaca aaacgaaaaa tgccgttacg taccggggtt ataaggaaaa agattttcaa
     6001 tccgttatta ttaaactcct gaacaccctt cattcaaccc cttcggaaat tgcacaattc
     6061 cggcaggaat acgaactgat tcaacgtctt gatatcggaa gcgttgtcaa aaccatcgat
     6121 attattcatt acaaaggcgg cctggcgctc atcctcgagg attttgacgg tattcccgtt
     6181 aaggactatt tccgcccgaa tgaaaaaatc aacatcgcat catttctcgc cctatcggcg
     6241 aacattgccg caacactggc agcgctgcat gcccggggag tcattcaccg gaacataaaa
     6301 ccccaaagcc ttctgatcaa tccggaaacg gaggaattga aaattacgga cttcggaata
     6361 tccgctctgc tgactcacga acatgatgaa gtctataatc ctgatttcat ccgcgatacc
     6421 ctgacataca tgtcgcctga gcagaccggc cggatgaaca ggtctgttga ttaccgcacg
     6481 gatctctatt cgctgggaat cactctgtat gaaatcgtca ccggtttgct gcctttcaag
     6541 tcgcaggacc ccatggaact cattcatgcg catatcgcca tgatgcccag ggcgcccgtt
     6601 aagcttgatg ccggtgttcc tgccatggtg tcggatatca tcatgaggct gctggcaaaa
     6661 aaccccgagg ccagatacca gaacggtttc ggtcttcaag ccgattttca ggaatgcttg
     6721 aaacaatggg aagagaaaaa aaggatagaa ccctttgaac tggggagaca ggatatatcc
     6781 aaccggttca tcgttccgca aaaacttttc ggacgggaaa aggaaatatc ggaactgatc
     6841 tccagattcg aggaggtagc caacggcgac aagagattgt ccgtgatggt tgttgccggt
     6901 gctcccggta tcggcaaatc ggccatgatt aatgaaatcc ataaaccgat cgtggctagg
     6961 cggggctatt ttatttccgg caaatatgag caattcagaa gggacaaacc ttacagcgcc
     7021 attattcagg cttttcaggt tctcgtgaaa caaatattgt cggaaagcga acaaagaatc
     7081 aatctctgga agaaaaatct tttaagtacg ctgggcgaca acggtcgggt gatcacggac
     7141 gtgattccgg aagtcgaatg gatcatcgga aagcagccgg atttgccggt tctgggtccg
     7201 gaagagaccc ggaaccggtt taaatttatc tttgaaaaat tcatgagtgt ctttccgcaa
     7261 caggaacatc cggtggctct ttttctggat gatttgcagt gggcggatat ggcgagtctc
     7321 caactcatga aaaacatcat gctcagcgac atccatcatc tcttcattct attttcatac
     7381 agggataatg aggtgaatga gactcatccc tttagcgaac ttctcagcga agttaaaaag
     7441 cagaacatcc gtatcgacaa aatcacactc gatcccttga aggaaaagga agtcacagaa
     7501 ttaatcgtca actttttacg gtgttcggag gaacggggat tcggactggc cgggcttgtg
     7561 caggaaaaag ccggcggcaa tcctttcttt gtgaatcaat tccttcatac gctttacaat
     7621 gaaaagatga ttgttcacaa gggagcgcag ggctggcaat gggatgtggg agaaatcagc
     7681 cggatgaaag tggcggataa tctcattcaa atgatggccg gaaagattga aaaacttccc
     7741 gacaatgctc aggatgtttt gaaaatctgc gcctgcatcg gcaaccgttt tgatctggaa
     7801 acattggcga cagtaagaaa aacatcggtg gatgaggcgc tggctgatct taccgaagcc
     7861 attaaagagg gaatggtcag ccgactcggc gatttctatg tctttcatca tgaccggatt
     7921 catgaggcag cctattcgct ggtaacggac gcggaaaagt cggcgcttca ttacaaaatc
     7981 ggcaggatcc tgatgggaaa tttagacgaa aaggaactgc cgggcaaact gttctacatt
     8041 gttgatcaac tcaatcttgg cgttcagatg atacaaaacg aggaagagag ggaaaaactt
     8101 gcgcggttta atctggaagc cggaaagaag gcgaaagaat cggcggccta tgtgcccgcg
     8161 tatcagtatc tgaaaaacgg aatgaatctt ctggaagaaa atccctggat aaaacaatac
     8221 cctcttacac ttgcccttta caccgaaacg acggaagctg cctacctgat gggtgattac
     8281 gacaagatga atgcgctggc ggaaatcacg ttgcagcacg caaagacaaa tcaggagaaa
     8341 accagaattt acacatcgag gattaatgcc tgtatcgcgc gtgaggacta caggggagcc
     8401 atcgacgtgg cgatgccgat tctgaatacc atagcgcccc ggttcggtat ccgactgccg
     8461 aagaaagcca ataagcttca tatcgctttg gaaatattca gaatcatgct caaacttgca
     8521 gggaaaagat cggaagatct gattaatctg cccaaaacaa gtgatcttga aatcctggca
     8581 gccggaaaaa ttctggccag tgtgagccat gccgcttttt acaccgaccc gaatttgctg
     8641 gctttaatca ttctgaaaac cttccggctg atattcaaat acggcctggc gccggaacat
     8701 gcctttgcgt ttgccggttt tggaatcgtt caggcggcag gactctggga tttcaaatcc
     8761 gctatcgcct tcggcaaaat gggactgaaa cttgtggaaa aactggatgc ccgcgatcag
     8821 gcagcccgaa cggtttttgt gtataacgcg ctggtccgac actggaaata tcccatgaaa
     8881 gacagcattg agcctatgat cgaaggctat aaggtcggac tggagacagg cgatctggga
     8941 tttgccactt tcaaccttta tttcacagaa gtacactaca tcttcaccgg cagggaactt
     9001 tccgatctgg aaaaatacat ggaaagaaac aacagaatca tcgccggtat gaaacaggga
     9061 catacattaa cgttgcaatc gatgacctgg caggcgattc tgaatttgct gggacgatgt
     9121 gataattccc tgaaattgac cggtaaagcc atcgacgcgg aaaagcttct gccctcatgg
     9181 gaagaaactg aaaatctggc ggctctttcg gttttctggt tcgtcaaatt aatcttgtat
     9241 tcgcttttca gcgagtacaa gatggcattg aaagcctctg atgaattcag aaaatacatg
     9301 gactcgcagc agggagtttt aatcaataaa tatgctgtat tactggattc tgtagccagg
     9361 ctgaaggtgt ataaaaatgc ttccctgccg gagaaaatca gacatcgcgt attactgaaa
     9421 atcaacgagt tgaaaatacg ggcgtgggca aaagccgggc ccatgaactg ttcatacatg
     9481 tatacggcta tcaaagcttt gtatgcatgg gttatacgcg gcaatctggc gaaagcggaa
     9541 aaattggtgg aactggccgt caggcagtgc cgggaatacg gcgattttgt tgtggagggc
     9601 attgcctctg aaatcatggc cggggaatat cgatcgatcg gagagaatga aaaggcaaaa
     9661 agattcctgg atcggacagg gatctgctat tccacatggg gagcgacggg cctgctgaat
     9721 aaaatcagga aaatgtatcc ggaactggtt tcctctgaag cggatgtttc tctgaatgtt
     9781 gaggcaacga cagccgaaaa aacaaaagat gatctgtcgg ttgcactgga cctgtccaca
     9841 gtgatgcagg tctcccaggt gatttccagt gaaatcatgc tggagcggct tctggaaaaa
     9901 atcatgcaca tgtccgttac cagcgccggc gcccagcgcg gttatctcat gctggagtca
     9961 gaaggaaaat tactcattga ggcaagcgaa gatattgaca atgatgaaac aaacgtcatg
    10021 caggccatgc cgctgcatga ctgtgccgat atttgccagg cgattgtcaa ctacgtccat
    10081 cacagcggga aagatgtcat cctcggccat gccgcaaaag aaggagcctt tacggacgat
    10141 tcctatatca acaacaagca gtgtaaatcc gtgctgtgta ttcccattat tcataaaggt
    10201 aaaatttccg gaattctata tatggaaaac aatctgagcg aagatgcatt tacccccggg
    10261 cgactggaca ttctccgttt catctccgcc caggcggcca tctctattga aaacgccaag
    10321 ctcttcgaac tggccacaac cctggaaaac atcgaagacg gttattggga aatcgatctg
    10381 gccggcaatt atacattttt gaataacgcc gtctgtcaga tccatggcta ctcgaaagag
    10441 gaattgatag ggatgaacaa tcggcagtat acggatgaag aaaccgcaaa aaaagtttac
    10501 aaagccttca ataatgtttt caaaacggga cagcctctca aggggctgga ctggcagatt
    10561 atccggaagg acggaaccaa aagatacgtc gaaatatcca ttttattgca gaaagattcg
    10621 tccggcaaac cgatcggatt cagaggggtc gtccgtgata tcactgaacg caaaaatatg
    10681 gaggagaaac tgcaacaaac gctggaaagc ctgagcaaag cctacgacag cacgcttcag
    10741 gtcatggtgt ccgccgtgga agtcagggat ccctacaccg cattgcacca ggtccggtcg
    10801 gcggatgtgg cgcggaatat cgccatcgaa atgggtctgc ctcaggaaaa aattgatgcg
    10861 atccgcatgg ccggggccat tcacgacatc ggcaagttat ccatcccgcc tgaaatatta
    10921 accaaaccgg aaaagttaac cagtgttgaa tatgctttaa ttaaagagca ttcccgcagc
    10981 ggctatgagg tcttgaagaa cgtggaatct ccctggccgc tggcgaaaat tgttcaccag
    11041 caccatgaac ggatggacgg atcgggttat cccagaaacc ttaaaggaga ggaaattctt
    11101 ctggaggcaa gaattcttgc tgtagccgat gttatttctg ccatggtctc gcatcgtcaa
    11161 catcgcccgt ctttgagtat cgaggtggcc cttgaagaga ttgaaaagaa taaaggaact
    11221 ctttatgata attcggtagc ggatgcctgc ctgagattgt tccgggaaaa gggttataaa
    11281 ctcaattaat taaggttatc aaaccattta aatcattttt ttcttgacaa aaaaacaaaa
    11341 tataatattt taacaggcga ttcgaacggt acgtttctga ttctcgcctg cacgttttct
    11401 gaccaaatga gacccatgta agaacatcaa gatgtcaaag ttgatgtatg ggtcgcactt
    11461 cttcattatt caaaaaaaat tttaaggggg gaattttatg aaacgtatgt tttggtataa
    11521 acgactgttt tttttatcct ttttgatttt gatggtagga gctttttggg cgatttcatc
    11581 agttgctgct cccacacagg gtccagccgg aatggctttt tatgagaagc catccactat
    11641 acccggcacg catggcacct taatccgtta ccgtacatca acatccgttc cgccgggcgg
    11701 accatcgtgt tattcatgga ccgtcatgta tgcgtcacag gatgctttgg gagaacccaa
    11761 cgtggttacc ggtacggtac tggttccgaa gtctttatat aattacggcc tcaccgcgcg
    11821 tcccatcatc agttatgcgg tcggcacgca cggtcttgtg cagagttggg cgccatcgat
    11881 tcaactggaa aacggaacgg attacgagaa ttcgaatatc gcggctgccc tcaacagggg
    11941 ctatgccgtt ctcgtcaccg ataacccggg ttacaccaac aacgatgtac cttcctacat
    12001 ggtgggcaag gctcagggac aggcagttct cgatattgtg acggcagctc tgcagatccc
    12061 caatatatct ctcaagtcct atgcaaaagt cggtatctgg ggatattccc agggcggtca
    12121 gtcggcggca tgggcaggcc agttgcagtc gacctacgca cccggtatta aattggccgg
    12181 ggttgcggcc ggcggtgttc ctgccgagct gttttctgtc gggagatatc tggacggcaa
    12241 caacgggtca tccttcctgc tgtcaacctg catcgggctg aacgcgcagt acccgaatga
    12301 cattcttctg aatagccgtg tgaacactga cgggatggac gcgattggtc gaggcctttc
    12361 catgggtgta ttcgaggctc tttttgcttt catgaatacg ccgctttccg atttcaccaa
    12421 atacggggaa acctccgatc agatttttgc gattccggaa attgctgaag tggtcaatgg
    12481 tcaggaactc ggaaaaacaa aaattaatgc acccgttttt ctgtatcatg gcacggggga
    12541 tgaatttatc ccgctggaac aggctctgaa tctcaaagag aaatactgca gccttggcgt
    12601 caatacatcc tatatggttt atcccggcga acatatcacg acgcaatttc aggccgcacc
    12661 acaggtgctt gactggttga aggaccggtt tgccgggaaa agcgcagcca gtacctgcag
    12721 aacttccaac cctcgcccgg catccacagc caatcccgtt gacggcgact ttcttttttc
    12781 tctcgatggt tggaaacttg acggcatgat caaactgaaa acactcatga cgaaagtatc
    12841 tttaccggaa ggatcaacgt tctcggcgga aacgaatatg acgaataata cgattacggg
    12901 cggtatggac atcccggaat tcagttacta catctacgcg tttggtttga tgccgttgca
    12961 ggtgaaattg aagatcgtgc ccgcaggaac catgacaggc acagcctcac tcgataaaaa
    13021 cggaatcctg catatcaacg gaaatgtcaa agcagacatt tatctgaaaa aggtcggtga
    13081 actgggaatc gggattccct tctcgctgaa aaccaaaacg cctgttgact tcccaatcgt
    13141 cttcgacgga cctgtatcat cgctgggaga tggttctctg acattcacgg gaaccacgac
    13201 attcccggac atggtcgaaa atgggattat tatcaatgcc ctgtttacgg gtttgatgtc
    13261 cggtcccggt caggaattta ccttcaccgt gacgccgcct gcgccggtag cgtggtaaga
    13321 ttttttatct gatttttttc acatctccgg tcaggctgcc tgacaatggc ctggccggag
    13381 atatattttc acgatatgat attttaatat cgtaacaagg caatgacaca aggagagtct
    13441 ataaatgacc cgaagcgata tgaaaaaaaa taagtccaag acaaaaataa tactcgctgc
    13501 gctgatagtc ggaatggcag tgattgcggg cattgtgtat tggcatagcg gcaatgacgg
    13561 cgataccggg gccgctgttg atgcaacaca acccgtcgca ttaaacatgt ctgaacaaga
    13621 catcgtggac gctgagaaaa atgcgtccat agctgctgtt tcgggagaaa cggttgccgg
    13681 tacggtaaca gaaagacctg attatataac cgaaatcgag tggcaggtgc ttcaaagcgt
    13741 aaccaggcaa aatccggata tcaaagtaaa cgatttggtc aacaaaatgt tgtttgccaa
    13801 aaaaaagcag gcatggcttt ctgccgaaaa agattcagcc gagcgcaaac ttctggccaa
    13861 gcaattgctg gatatgattc cgggtcaagt tgctatggag gccattgatg gtgcaacagc
    13921 ccaaacaatg gaagcggaac ttcatgcgga cttgttgcga taaaagatcc gcaaatccaa
    13981 atccgaaatc cggggacact ttagagtttg ttggaaaagg gaaatcggtc aggttgttct
    14041 cctcggcatc atgagcacgt ctttgttgta tccgggcatc aatcatttct gtgagatgaa
    14101 ttgcgttttc ggtattgccc ctgacgggga tgcaaaagaa ttttacggta aggttgccaa
    14161 aaattttaag gcaaggggat tctaaactta cacggattga ataaacgatg aaagaggctg
    14221 tccgaccggg atagccccgt tgattttttt cacacaggca tcaagaaaga ctctcatgag
    14281 gcggagattt tcttaccggc cggtttctgt tgccgttttg ggttcttgcc tgcgtaaccg
    14341 gaatacgaat ccattaagct ttcgttcgtc gaggggagac ccgtcgttat aaacgaaaag
    14401 cgtcggaatc tggcgctgat gtttgagcag gctttcggca atcagtgccg gaccgcaccc
    14461 ccatggtcca acaatcacga taccgtctga gaccgcctta tcccagtgat gaataacgct
    14521 tcccacggtt atcggggcct cgccacgcgg gtgccggtcg atgatcggtt ttgctttttt
    14581 cagcatttcc ggaacatcga atgtgggcag ccaggggttt tgttcctgag ccatttcgta
    14641 aaagtgtttt cgcgattttg gcaggaggac cttcgccatg gcattcatga aagttccctg
    14701 aggcagactg aataattcgg cggaacgttc ttcaaccaga tattcggcca gtacccataa
    14761 aggttccacg atgacccgta tcccgcgagc attcagccgt cggataatgg cgtcgcttcc
    14821 gaaatcatcc agacgcagat agatatctcc cgtaaggaaa atcgtccgga gagaattttg
    14881 atccgtccga cacttccgca tggctgcaaa ctgtgtcgat gcgcggcgaa caagttgctc
    14941 gaaaagagcg ctgccttggt tctgctcaat acttccggag acgatccgtc cgacagatcc
    15001 catctttgaa atgattccgg agaaacctct tccggaaggt ttttccagta tccggataag
    15061 gtcgctgcaa tagtcatcgt atatcctgtc gacttctccc ggttcggatt cataagggcg
    15121 gtgataggca gcaagctggt tcagaagatt ccatacaagc gttcccgtgt aaaaccggct
    15181 gaccaacccg gggcattcca gctcggcggc aacatggcgc agcgacacac gatctgaaag
    15241 gcccatattc tcgatcgata tctgatcttt catggagaaa ttgcaggtgc ggcaggcgcc
    15301 ccttccgctg atttgaagca ggaccgtgcg tttctcgggc gggttgtcat cgagatattt
    15361 ccgaaacgaa ccccagatca actggtaggc aaggcactct ttgcctgaac agtctcttct
    15421 ccccgcagcc agagacactg ctgatgtggg aattgccggg atcgcgtcat agccccacga
    15481 ccggtagaca gccgccataa tcggagccgt ctggtcgccc atggtaaaga cgacaaattt
    15541 tgaatccttt tgcgatgcaa gcggcgggcg ttccagtgtg tcgaacaggc ggagtcttga
    15601 cgattctgcg ggaaccggcc ggccgtcatg ctgacgaacg gtgtgcagga aagcctggat
    15661 tcttgtcacg aaacccgcaa agcctccgtg cccgtcgctt tccagagccg tatgcggata
    15721 accttccatc aggaaccgaa agaaatgttc aataaacgag gccggtccgc agccgaaaga
    15781 ggaaagaaaa agggggaaca cctctccgca attcctggct gaaagcgcga cacggagagc
    15841 cctgttcgca tcttcccaaa cgatgcgggg aagttcaggg atatcgtccg gaatcggata
    15901 acagtccatg ggcagcgaca gtacgccgtt ttgcctgaga agatctggaa tactggcatt
    15961 gaccgcgcga tcatagatca catgcagcga accgatgacg gcaaccaccg gaattccctg
    16021 tccccggccg taggccaggg ttcgccgccc gatttccgcc agcgcatgtt catagtcctg
    16081 ctgcgctttt gccgcgcggc gggccgctgt tttgacatgt cgatcatcga cgcccagttc
    16141 ccgggcgagc cctcgaaggc ggccgatgag tcgccggttc agcaggccgt ctctcaggtt
    16201 gagaaggggc ttgacgaacc tgatattccg gccgcgagct ttcagcgcct ggtcggccat
    16261 gtccgccatg ccctgctcca tcggacacgt gcggccgcca tggccgtccc tgtcatgcag
    16321 attcagaaat ttcgggaata caagcgtgtc aacatccgca tccgccaccg cgtgggcgat
    16381 tttagcagga gcgcaggaat cgaacgaaaa acagcgttct tcaccgcccg ccagcgattt
    16441 gctgtcagag cgtaacacct ttacacggaa tccgatttcc gacagaaaag tcaccatcca
    16501 gggcaggtat ccgttcagca ctccgacata aggcaccccc acctcccgat cgcctgtccc
    16561 ggcttcccgg aaattttcca gcagcgccat tctttcttca agcgcgttcg gggcctcctt
    16621 gggaattttc tttgaagcga ttgatgagat ttcgtatttc gggcaggcgc ctccggacag
    16681 aacggtcttt tctgctgcgc cgacagcgac agtcgtttta tcaatatgac aaaaattatg
    16741 acatttggaa tcggcacacc ggaattctat tctggaaacc actctggcgt tgagcgccag
    16801 attcagatcg aaagtgccgg ctgcgaaaag tttttcttcg cccagctccc ggattgcgca
    16861 cagaccgatg ccccaagcgc ccatggcccc gggattatgc ggaacgatta cctcccgacc
    16921 cgtgaccgcg gccagagtcc aggcaagcga cggtccgctg gctggcttgc cctgaaaaaa
    16981 gatccggtcg ccgaaggtcc gtcctcccat gacccgattg atatagttgt ggatgaccga
    17041 atactggaac ccgccgaaca aatcttcgat gctgaatcct tcgttgtgcg cttcagcggc
    17101 agcatcggca atgaaaacgg tacacatctg tccgagatcc ggcggacgct ttgctttttg
    17161 ggccagcctt gtgaactctt ttacatcgtg tatatcgtaa aaaacggcct gttcttcgag
    17221 gaaagaaccg gttccggcac tgcatgcctt gttcatgtcg ctttcggtaa tctgaccgcc
    17281 ggcgatctgg atgaacttgg catcctgtcc tccgatttca acgatggaga gacttttccc
    17341 tttctggtca tcgcatctga tcgcagctgt tgcatgggcg acaatttcgt tcagaactac
    17401 aatccgctcg gcaagctcag ggaacaccgc gcggagaacc gttgcggcgg cttcacgtcc
    17461 ggagccggtc agggcaatag cccgtacgtc cggtttcgtc tgcctgagaa tctcgtccag
    17521 cagacgcagc gaagcatcaa ccggattccc gcgagtacgg tcataaacgt ctgtcaactg
    17581 gttgccggtt ctaatggacg ttagcaccgc cttgctgccc gtcgatccca gatcaaggcc
    17641 tagaacggcc ggtgtgactg ccggatcctc cgtgacatcg ggggctgact gatacgtgac
    17701 gttcgatgcc cactgctgag ccggcgactg aatgcgaaat cttgttcgcc gcggaatgat
    17761 tagctgctcg ggatcaacag gcagttttgg gagcttgtct tcccggtact ggtcggctgc
    17821 aagtaccgct gcaccgaggg cctcgaaaag ttgcggttca tcaagcacaa aaaccgtgcc
    17881 gccggcgatt tcccggaatg ccttgacaaa agaagcgagt cgcgcaccgc ctcccataag
    17941 ataaacgggg ccttcgatgc gtaccctcga cagaagggct gccgtgttgc gggcaaccga
    18001 ttcgaaataa cccttcatga gctggtcggt cggtttcccc tggttaccga aatgggtcat
    18061 ttcgcttttg gcaaagaccg aacaacgggc cgagatggga acgctgactg cagcgccgaa
    18121 agccagttga tcggcttctt cgagaccgag tccgaaacgg ccggctattt ttacaatggt
    18181 ctcgcccgtt ccagaggaac atttgtcatt ctccacatag ccgacatgtc cctgccgatc
    18241 tctggtcatg accgaatatc cgcgggcgcc gagacttacg agattaatct gttccggcag
    18301 atcttgctgc aattgcagcg ccgccgaaat acaagcgtct tccggcagtc cggaaatcac
    18361 cggagcgacc agttcctcgg agtgcagacc ggtagcggcg agagctgcac atgaagcgat
    18421 atgcttttca cggtaccatt cgcggaaagc attgagcgga ttcccatgat ggacgatgca
    18481 gcggaaatct tccaggatcg gtgtgtcgct gtcacagacg ttgagcatca caaatttcgc
    18541 ggtggatttg cccagatcac agccggcaac catgacggtt tctccttgtt ttcccgaaaa
    18601 cgatttccct ttatgatgta aatgatggct cttcattttt ttctccgctt gaattcgaca
    18661 atttcagaaa aaaacaacat cgataagatt ttccttcttt taggaacggg ggttataagg
    18721 aacatccgcg attgtgtcaa ggaaatttac cccgaaaaaa aacggtcaca acggaaacat
    18781 attggcgatg acggatgctt cccggatgtg ttttgaatcg cggaataaaa ttattcaaat
    18841 cttaataacg gtttaatttc ttttaatcgc ttgttttttt atgggttgat cttcgatcaa
    18901 attcatcaac agaaaataat caaattttgc gtctgcctgc aggccgatgc gttgataaag
    18961 ttgcttatgg gtaccgctga aacggacatc cctttttaaa acttcggagg aagagtaacg
    19021 tttgatttgt gtttctcccg attcgttttc tatgacgtca atccagccgc cattttcttg
    19081 acggtaacga caggctttcg atccgtcggc aaggtatcct ctgttttgta aaacgccttc
    19141 cggtgctaaa aatatcagct taatatcctc catcatattg ttggcgaaat ctttggaatc
    19201 aaagggaggt agggctcgtt tgacgttgat cccatgaggg tttgcttcgg cttcaaacag
    19261 cgacatgcct tccaccgtta taatcgcgca ggagaccgaa cgggtatcgg ggagagccag
    19321 cgttatgccg ataatctgac tttgtactct tccgtcaaga cgggcctcga tggcatgcac
    19381 aagacgataa tttctcttta aaaaaggatc agggcaaacg aacgattttt ctttacgtgg
    19441 agaagaaggt gcatttatga tgggcagggt ctggcaggaa gacagaaaaa aaatcactat
    19501 ccaaagaaga tgtcgcctca tcgtacatcc ttaaaaatgg tcggatcgat cgcgtgattc
    19561 attttaacat tactgaaatt gatctgggtg atgttatcgg gtccttccag aattttgacc
    19621 gattggacaa cccgcgcttg gggcgcgaga ttaatttcga tgcgttcaat cattcctttc
    19681 attttttttc ccaccggtgt caggatgatg caggtattct ctttcccgat gacggttgct
    19741 ttaaatgatg gattctgatc gaatctgccg ttcatccatc cggcaatttc attcagaacg
    19801 attctcatgg cctgcacgcc atttgtttta tcttcgaccc atttcccatc ggaatgcata
    19861 tagcgcctgg cctgctggtt gtgactcagt acaacgcttt tcagaggctt aatgtattcc
    19921 caacgcaaag aatcaggcat tgcaaaataa aaataaccct gagaaacaag tggcctggat
    19981 agaattttca tgcttttttt ttgcgtgaaa tctgcctgga gcgttttgat ttttacagat
    20041 tctttgcgga gcttttcaaa acctttttct tccgcatgac aaacgttgcc gtacaggaaa
    20101 cagaatcctg tcagaaggat catgaacaga gatatcttat ggtataggcg catcagcata
    20161 tgccaccatt aatgccgatg acctgaccgg ttacgtaaga agcatcatcg gaacataaaa
    20221 agcggatgac gctggcgact tcctgcggtt ttcccaacct ggccatggga atgatttgtt
    20281 taatctgatc aaccggagcg tctttgatca tatccgtttc aatcaagccg ggagcgacga
    20341 cgttgacccg gactccgaaa cgggccactt cggacgcaag tgaacgcgac gcggcaataa
    20401 ttccggcttt cgcggccgcg tagttggcct gtccgcgatt gggcatcaac gccgaaagag
    20461 aggaaacgga aacaatggag ccgcgttttt ttctgatcat ttcacgcaaa atgggtttag
    20521 tcatatgata aaaaccattc aagctggtgt tgatgacact gtcccattcc tcttcgccca
    20581 gtaacataaa cagcccgtca gcggtaatgc cggcgttgtt aatgagcgcc tggatatttt
    20641 tatattgttc ggtgattgtc ttaaccgcat ttctggtctg ttccgaatcg gcgacatcga
    20701 atggaatgat agtgccatct ccaccttctg atttcaccat attcaatgtt tcctgcgccg
    20761 ccgtctcatt ggtcttgaag ttgatgatga cgaaatatcc ggcccgcgcc agttgcactg
    20821 ccgtggcgcg tcctatccct cggcttgcac cggtgataat cgctgttttt ttatcgttca
    20881 tattttttac tccccgtaac aaaattattg atcgttatca ttgagacgca cggcctgaag
    20941 gacggctgtt gcaagaattt tttctcccgt tttaaccgtt ccctctacga cgccgtaatt
    21001 atcaaaagca tctttgtttt ccagcagaat aaccagatgc gtattcaacg gtattttctc
    21061 ttcataaaac gcggcatttt tgataccgac cagccagccc ttgacggcgc cggcaccccg
    21121 ctgcttcctc ttgtatcctt ccacaatggc agccgtttgc gcaattcctt cgatcaacac
    21181 cagcgcatca acggtttttc catcggaaag gggccagtcc ggaccgacca tcgcggcgct
    21241 cacagcagaa ttctcctgta tatcaatgac gtgattgata atttttatct ttttgcggtg
    21301 cggaatcaaa ctgtcaatat cgatgtcaag catgggttga tgtcatcaaa ttttttctta
    21361 aactaatgaa aaaaacatgc ggaagtcaac aagaattata ccggctttga taataggtgc
    21421 tttttcctcc tttttacggt aaagaaataa aaaaatcttg agctgcctga tgatgacgca
    21481 agcaaaaaaa gatccttttg tcggcctgta caaaaaaatg ctggaacttt ccccgagcgt
    21541 ctgccggaaa gattttacct tcggcggata cagttacggc gatgttctgg cgctggcggc
    21601 cggcctgaag aagatactgg acaggcgcag cggcaaaaaa aatatttgtt tgtgcagtgc
    21661 gaataaagcg gtaacggctg cctgcgcgct ggcggcgttg agcggttcct gcaagctggt
    21721 cctgccttac tctttttcgg cccatgccat gaaagaaatg tccgaagcga ccggtttcaa
    21781 ttatgccatt gcggatcatc cggaagaaat gccggagggg gttgaaatca tcacgccggt
    21841 tcctgctgcc ccggaaatcc tccgccccga atcgctgatc catccggatg aaacgtttct
    21901 tcggcttttt accggcggat cgaccggcaa accaaacgtc tggtcgaaat caccccgcaa
    21961 cctgctgtcg gaagcatttt atttgaagga aaggttctgc ctgtcaacca aagatctttt
    22021 tattgcaaca gtaccgccgt atcatattta cggtctgctt ttcaccgttt tccttccttt
    22081 tgttgcccga gccgccgtgc ttccagatat ctataccttt ccgcaggaga tcatttccac
    22141 aatcaacaaa cacaaagcct ctgtgctggt gtgtgtcccc attcattacc gtgttctgaa
    22201 ggttgacaat ctgtcggttc cttcactgag agcggcatat tcttcttccg gaatgctgga
    22261 tcgttctgac agtttgtatt tttaccgaaa gacaggcctg ggcattacgg aagtttacgg
    22321 ttccacagaa accggcgga
//
LOCUS       DAONQZ010000038        21867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2682_length_21867_cov_16.785577, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000038 DAONQZ010000000
VERSION     DAONQZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21867
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_21867_cov_16.785577"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PLR13_07450"
     CDS             complement(<1..1444)
                     /locus_tag="PLR13_07450"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HQC19209.1"
                     /translation="MKTMQAALNYAGKNNYSIIPLQRNKKPYFQWTDFQHRKASSDEI
                     KAWWSKWPNAMIGIVTGQISGLFVVDCDTQKGFDTVQELLPDSLEIPTARTPRGGWHL
                     YFQYPTDSGLTVQAAIMPGVDIRGEGGYIIAAPSMNGNGKGYEWLDGLSLAEITPPPL
                     PNALLSAFNNIINNSIYRGEVKTEVSASKMFENGRRDNDLFHVSNCLVKGGMPKEEIE
                     QVLEKLIFSWGENPDSKWIQAKIESAFQRANKRERNLAKEVLDFISVTNGYFSVTDCY
                     TVLQVVTKEDKTAVRVALSRLKDKDKVIEKHGNKDGVYRRIENEIEEVNFLNAPTDEF
                     SISLPLGIDSYCKLYPGNIVVVAGSKSSGKTAFLLNIVKENMKRYEVVYLNSEMGDTE
                     FRKRLELFEDVKLQDWKFRAYHRMANFADLITPEKKIFIVDFLEVTTDFWKVAQYIQE
                     IHRKLKEGICIIALQKSEGKDNGRGGDFSKE"
     gene            complement(1401..1562)
                     /locus_tag="PLR13_07455"
     CDS             complement(1401..1562)
                     /locus_tag="PLR13_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19210.1"
                     /translation="MTEKEIIKRKIFLKRQLIQITEKEIKILEDELNHRGIIGHEDNA
                     SGFELRREK"
     gene            complement(1559..1780)
                     /locus_tag="PLR13_07460"
     CDS             complement(1559..1780)
                     /locus_tag="PLR13_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC19211.1"
                     /translation="MQDQESLNVKEVTGEELLTGDELCHKLKIKKSFLYAPVRRKGPN
                     AIPCIRVGKYLRYHLQTVQAHFAKQNEAQ"
     gene            complement(1904..2623)
                     /locus_tag="PLR13_07465"
     CDS             complement(1904..2623)
                     /locus_tag="PLR13_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19212.1"
                     /translation="MSKLNVRNIHSPNLDDHIYHLPNEGPGYDDLKQLYAWSFDDAWK
                     AQNEWKAKNTRGRGPLFRWLGAQELKELYNTHKAGNSAAILEAIFLCSMNSLPIPRWC
                     EMAYITAYRKVRQYKAKSWDDVFGRPHPKGMHLETKRQEREKGFKVYDMIEKIKQDDP
                     TTPIDGFLFETIGRKLGIGGKTLTETYYYKELTFRKRYKCDGCGKRYAHWHDINKDGF
                     CNYCEDKKKALDKNKTIKQSP"
     gene            complement(2740..3474)
                     /locus_tag="PLR13_07470"
     CDS             complement(2740..3474)
                     /locus_tag="PLR13_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19213.1"
                     /translation="MKKIALFSILICLFFIQGCVRGQWINTKFSPINPTSGGYIKNYT
                     LGEKRTAFIGQEIIKVQKCDFFQKTFISLQDISAAAKYRASNLSIVHSKDNHQYIIHG
                     TVIFNDKTHYVTRGSDGSYTWGILVLDDGSIINNVLYSYDYEMLYLSDSIIITPSKLN
                     YSHSCVRGTPSAISCELIFAGKNNVSLNTTYKEYSYNDLARPAFFQNLTYQANAQQIR
                     FKDFLIQIHDVTNEQITYTVLEDGLK"
     gene            complement(3755..4054)
                     /locus_tag="PLR13_07475"
     CDS             complement(3755..4054)
                     /locus_tag="PLR13_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HQC19214.1"
                     /translation="MIHIIPVNKLSARALRGVIEEFISRAGTDYGAVDASMETKFKQA
                     KASLLNGSVVLVFDDETETTNMFRADDPILKKIDAIPEDTETQEAILAEMIKPEK"
     gene            complement(4071..4376)
                     /locus_tag="PLR13_07480"
     CDS             complement(4071..4376)
                     /locus_tag="PLR13_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19215.1"
                     /translation="MNAKQGLTDMGTSHDVGRTTGNMNMPDFSEKQNRRGIDRMQISG
                     YPLIPEVAQTKYKIFVNRFFREHVKLIEHEKCNLCLNDVNYFINLLESTVEHYHFND"
     gene            4469..>7638
                     /gene="recC"
                     /locus_tag="PLR13_07485"
     CDS             4469..>7638
                     /gene="recC"
                     /locus_tag="PLR13_07485"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HQC19216.1"
                     /translation="MGLHVVFSNRLEILKDILLEDLSRIPADPFEAQHVVVPGTAISR
                     YLQLAIADEKGICANVAFDYPGSWLWKLARLVDDKVPERSAVDPEIMTWMILRILGDN
                     RLAAYPRLAGFIQKADDLMLFELARSVAHVFDHYATYRPDWLVAWSEGKRIKDFREEC
                     ADEAKVYEEWQMEIWRTVTQELKLGSTHPLKIFLDKIMTGKTPGAASDLPGRAAIFAL
                     PVIPPIYLQTIRRLSEVMDITFYLLNPCREYWFDIVPPKQLAYLKKIGRDAHTEVGNP
                     LLADWGRSTQSAIDLIYDDASSANTLETSRFIEPEGQALLAQLQRSIFNMQELDPGSV
                     QLPHNDRSVEIHCCHGTVRELEVLHDRLLSLFCADQTLSADDVVVLTPDIDELAPAVE
                     AVFGTVPKDRFIPYTVSGRAMAGTNPYLNMLMGLLDLTASRMPASLVFDLLSQPLVAS
                     RFGVDNDRLNSIRSWLNGSGIFWGIDGEHRRELGLADEDRHTFRRGLDSMLLGVAIPC
                     LDEPIAGYFPYEDLEGSRAETLGQLWLFVERLAFWKNRLKNPIPANDWQDLLNRMLAD
                     FTECGRDAQGAYDQLVGAIAELADNWRAANLTKAISSRVIKAALADADVRRRGAVPSG
                     TVTFASLSAMRGIRYRVVCLVGMNDGSFPGRAYSPEFDLIPKAEPRRGDRQRNLEERG
                     IFLDALLAAGEILHISYTGRDQRDNAIMPPSVLVAKLLDYLSLAMAPENATANDLATA
                     RRQLTVDHPLQPFSRRYFDHSDPRLFSYVGQYATALNRGSKSTPVKKKADLRSDEQEE
                     DMPDPKTPFFNGYGAPPKSPDTESTTLTVDDLSYFLMNPSRFFLRRQLRIQLPQEEDV
                     IADEEPLLFEFSEERDAAAMIIEACRKQKRVITIQDALAILQASPSSPPGSACEAALS
                     GIWPKVSLLAERLLSATTGEKLQPVYSLLSIDVEGETWQLNADLMELRLCGLVFYRCD
                     DLRDFDHLNAWVRHLVLCVCRPEGVLPQTRHVALDGDLIFGDIAWEQAAKHLGNLLAL
                     YRDGLFEPVPFFRKSAWKFVQKDMNAA"
     assembly_gap    7639..7738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7739..8013
                     /locus_tag="PLR13_07490"
     CDS             <7739..8013
                     /locus_tag="PLR13_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19217.1"
                     /translation="PFFRKSAWKFVQKDMNAAKSKWSGGFNAGGKAESADPWHALAWR
                     GLEADDVLNERFKEIAHCVFAPILANEKLEKIVTEEEKGEKRGRKK"
     gene            7997..>9968
                     /locus_tag="PLR13_07495"
     CDS             7997..>9968
                     /locus_tag="PLR13_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQC19218.1"
                     /translation="MAGKNKKVEPLDVFTCDLNGIANIEASAGTGKTWAICGLYVRLI
                     AEMDLPVDQILVVTFTNAATAELRSRIRDRMVEVQHCLDTGRCLSPDPFAAGFLSRMD
                     RLGIRAKAGENLKNALLSFEESAIFTIHSFCQRTLGELPFSAVQPFNVEVEADDSEII
                     RETAADYWRCMTQHYTADEINALLQNKINPSWIAGEIRRLLNRPLAKLVMPETNPSEN
                     PASRRAVWIKRFILKTAPRAIRKTKQDRHVISYNDMLYNLHDVLTLGQFPWLAGVLRK
                     RFPVALIDEFQDTDPLQLEIFRAIYFDKGTLFMVGDPKQAIYSFRNADLNTYLAARRQ
                     AQRQWTMIHNQRSEEKLIFAINAVFGANKNVFGDGEKIPYRDVRRGTKPLKELQGDPE
                     PREPFSVWLFKTGSKAEPLKAGDARSLALAATAKEMARLIDGGMRGTIQIGDENLQPR
                     DIAVLVRTKAQGRQVKEALADYGIGSVDLTERSVFATVEAEEMKTVLRAIAEPSQAGL
                     LRAALSTMLLGFGSRELADLNADEAEFNRIVERVNEYRQAWLGSGFGAMWRRLIAQER
                     VIERLLPLKNGERKITNLMHLMELATGAEETHRGIEALLRWLSEKRSAGDEEDVAQLR
                     LESDENLVQVVTKHKAKGLEWPIVFCPFVWSDR"
     assembly_gap    9969..10068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10069..10961
                     /locus_tag="PLR13_07500"
     CDS             <10069..10961
                     /locus_tag="PLR13_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQC19219.1"
                     /translation="LPGVAWKISSYSSLTASSLEGGRQHEPATQDYDVIPLAIEDHDP
                     PQVLRAKLSGDIIDFPAGTTTGHCLHAVFERIDFQDESGWKDIITNVLANYPPVDALE
                     KRDDQVSRIHGMLKDVMSTPLPTGFKLGDVARAKRFSEFEFAYSVRHLDHAKLKNLLD
                     KHGCRIPPLDFPDLEGYLRGFIDLVFEHAGKWYVLDWKSNKLGISAAEYSRSSVDEAM
                     FNHAYEFQGLIYLIALHRYLKLRISKYDYSRHMGGILYLFVRGVRPAWPSAGIWHKLP
                     EPALIESLDALFSSENRRTV"
     gene            10967..12784
                     /gene="recD"
                     /locus_tag="PLR13_07505"
     CDS             10967..12784
                     /gene="recD"
                     /locus_tag="PLR13_07505"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HQC19220.1"
                     /translation="MRKHENIPAADYASPLSRGLADFLKRLSLTLGATEEAARTAGDA
                     ARACAQALAEGDVCANFSLLAAAEGKPAQDVRKLLDASLVASSAGADKKLPLVYDDEG
                     QIYLYRYFAYERKLADAIIRRLPGSAARTVSAESRQFLEKRFAENARKLSGRPDWQKI
                     AVEMAFTNRLTIISGGPGTGKTTIVTALIAALAINDHPPRIALVAPTGKAAARMEESM
                     RSQMSTLDAELRKRMPERASTIHMLLGASPAGNTFRFNENNPLPYDLIVVDEASMIDL
                     SLASRLFSALHPDTGIVLLGDKDQLAAVEAGAVFAELAQQTSVPDNMDKLSTGSGTPP
                     LNAQPNEKGPLSSCVVWLKENYRFSAASPIAKVAGLVVNGEGEQLIEWLQNEKGDNIK
                     WEAFDEGLPSRVVGALVKGFEPFVEAVNQGDPEKVLAAYEKFCVLCVIRNGRRGVNGI
                     NDVIARQLRLKISGEGPGEGPWYDGRPIMITENDYGLGVFNGDIGVALKTKDGHRRVW
                     FMDKDGGLRFVPPSSLPAHQTAFAMTVHKAQGSEFENVSLILPEQDTPVLTRELIYTA
                     VTRAKKTLLIYGSADILKLAVGRPTSRRSGLAKKISTPP"
     gene            complement(12931..15597)
                     /locus_tag="PLR13_07510"
     CDS             complement(12931..15597)
                     /locus_tag="PLR13_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQC19221.1"
                     /translation="MINIFPENDYNQTLRIKRFLIAFGAYVMWNAITIASYLLGLTKV
                     PLSILVAGVTANIVVNILLYAVFRTGFNKKAKDPSLTLLQMVIATFWIMVVMYYAYEA
                     RGACLLVYMVVLVFGFFRLRVRQFLLLSLYALVNYSAVIFLVYKLHPELINPKLDLLN
                     IIVLAIILPWFAMIGGYINGLRASITNALTTIERLTDNIKDVIFVVDMNLIYTYVSPS
                     VKTLRGYEPEDVMKQNLADSFAPTSLDTAMETLSEMMEMGKSKSQDSPERTLLLEINR
                     KDGNPVWTETKFSLVRDKEQYITGVLGVSRDISERKRAEEQYRLLADHMTDQVWLFDL
                     NLKFQYFSPSSEKASGYTFTEIKEISLDKLLTEESFQKALEMFSEEMAKAEIVPPPPD
                     YKRSLEVEFRCKDGRLISLDTTLSFIQDKNGKPLSILGESRDVTERKRAEAQIQYLAT
                     HDNLTQLPNRMMFNHLLNQAIKSAKRNKRQLALLFIDLDRFKIINDTMGHDAGDRLLK
                     EMAKRFRQTLRAADVIGRPKDKEDVVGRLGGDEFIILIEDVTDLANVAIVAQKILGTV
                     MKPMVLLGEECRVTSSIGISVYPTDGEDEQTLMKKADMAMYFAKEEGKNNYQFYSQNI
                     QSQSYERFSIETNLRRALERHELYLDYQAKMDFKSGTITGVEALLRWNSPQLGSITPT
                     QFIPVAEETGIIVPIGRWVLKTACVQNVAWQRQGLPPIRVAVNLSLRQLMDDKALEDI
                     KNALDESGMSPNLLELEITESMIMFKPERLIGVLTKIKEMGVRLALDDFGTGYSSLSQ
                     IKHLPIDTLKVDRSFIRNLPHDSDDKAIIEAIIAMGKTLSLNIVAEGVETLEQDHFLR
                     EKICDEMQGFYFSKPVAPDQFADLLRQYNFPS"
     gene            complement(15651..15944)
                     /locus_tag="PLR13_07515"
     CDS             complement(15651..15944)
                     /locus_tag="PLR13_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19222.1"
                     /translation="MSYCNPFQRPLVVRKSRAGPACTDVHTPENFFDAHLYYQVLNMS
                     MMISLEFSNAQQQASFVIRLLITGNAMGPLQSRLAEGGRGNPGLLNDLLNNFF"
     gene            16049..17104
                     /locus_tag="PLR13_07520"
     CDS             16049..17104
                     /locus_tag="PLR13_07520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQC19223.1"
                     /translation="MRIKRFFIAFSSYVMICSLVILGYILGFSPVPLRVLVVSQLGIL
                     ACNIILYVMFRTGLNKRFKDPSLTLLQIVIATCWLLEVLYYADSVRSAVLMDFLIIFI
                     LGLFKFNVREFLFLSFFTVAGYTGVILLLYINRPESLNYKVEILNIAILATVLPWFSL
                     VGGYITRLRKKVALSLATVKESEKKYIELSIIDDLTQLFNSRHFYAQLEKEIERANRY
                     NQPLTLLMLDLDKFKNFNDTYGHLEGDQVLSRIGHVIKRCLRDSDSAYRYGGEEFVIL
                     LPMTKAEAGITTATRIQETLRKTSFSPVSDQEIFMTVSVGLSQYIPREDMKVFVRRVD
                     KIMYQAKHQGRDRICTG"
     gene            17295..17501
                     /locus_tag="PLR13_07525"
     CDS             17295..17501
                     /locus_tag="PLR13_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19224.1"
                     /translation="MTYKELLEKQIGRECTFTASHTKCVINFGAAEPFASEGIIENVT
                     DDYVIIKSQSSTMIYLLVNTSISY"
     gene            17743..19035
                     /locus_tag="PLR13_07530"
     CDS             17743..19035
                     /locus_tag="PLR13_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19225.1"
                     /translation="MTSFAYGNVVQESDAQVWKVGRHRWTIHDEYRYGIWIEESVTED
                     FFIRHNIRVDCADVPYAMRWIYARIHHLPAAASTVDNRFIGHWSKDWSHLPTNPHWHK
                     DRRFRAALMSVLSSTSTQTLPSDTYPIKIGRHSVTPGTVFLFARDHAGIVSHVVMDGS
                     TTHPVQTFEASSPARLQGLRLKDFLLPNPNADYISGLLKFRWPVSDGNTWRYLPLEEQ
                     PFYSDEQYLPAFTKGYSNYLEAVEKRINPAVYEPGEKAEKIMMTLYRRLNERVPIVLK
                     GYIKCHGIECPEGSLLWEIYSTYNRDDFIGFLLHYLEQIIARNHLDRNDVLHKMEGIR
                     LQITPGRFVTLRHVLENFKWIASDPEAGIEARWGLDKCSTIATRLRTAEESIVFIQNK
                     YGETDPLFAEQSILKQQKIVDEMTSESLENNCMKNALR"
     gene            complement(19096..20625)
                     /gene="guaA"
                     /locus_tag="PLR13_07535"
     CDS             complement(19096..20625)
                     /gene="guaA"
                     /locus_tag="PLR13_07535"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQC19226.1"
                     /translation="MILIIDFGSQYNQLIARRVRENHVYCQIEPPGISIAAIREMEPE
                     GIILSGGPASIYSKGAPRVDKKIFKLGIPVLGICYGLQYMIDSLGGKVISSPKREYGF
                     AELLVKTQKGIFRGVARKTPCWMSHGDSIGELPKGFAVTATTANTAVAAAQDVRRHFY
                     GLQFHPEVVHTRDGRIMLANFLFGICRCKKTWSMQSFVQEAVNDIRRQVGREKVLLGL
                     SGGVDSSVAAVLLHKAIGKQLTCVFVDNGVLREGEAQRVIGMFKKHLQINLRFARAGK
                     IFLKKLKGVTDPERKRKIIGRTFIEVFNREARKIKNVKFLAQGTLYPDLIESCSAFGG
                     PSAVIKSHHNVGGLPKTMSLQLIEPLKHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTPQRLSILRKADTILWEEIRARKLYYKLWQSFAVLLPIKSVGIMGDQRTY
                     ENILSIRAVTSDDAMTADWAPLPHDVLARISNRIINEVRGVNRVVYDISSKPPSTIEW
                     E"
     gene            complement(20891..>21867)
                     /locus_tag="PLR13_07540"
     CDS             complement(20891..>21867)
                     /locus_tag="PLR13_07540"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HQC19227.1"
                     /translation="RLGANEAPPAIISVFLGEKLTQILENIQRGKVTKATNADLIDLG
                     IFSLPTLSKDNTDRNRTSPFAFTGDKFEFRAVGSSQNISFPAMVINTIVAESLDELAE
                     KIKTKNSKNINQAVLEVLKEEISYSKAVLFNGDNYSREWEIEAARRGLPNEKTTPTAL
                     KALTTVKALNLFEKYQVLSNPELKSRQLIHVDRYIKDLEIEVKCLNTLCTSQIIPAAV
                     VYQKKLADAINSTRQALGNAASVTAEIELLKKITDLINSIHATNKEIQVKIEAANALH
                     DEQKKAEMLSAKVKPMMEELREYADALEILVDDDIWPVPKFWEMLFIS"
BASE COUNT         5368 a         5341 c         5314 g         5644 t
ORIGIN      
        1 tttccttgct gaagtcaccg ccccggccat tgtctttccc ctctgatttc tgcaatgcga
       61 ttatgcaaat gccttctttc agcttcctat gaatctcttg aatgtattgt gcgactttcc
      121 aaaagtccgt agtaacttcc aagaaatcaa ctatgaatat tttcttttcc ggggtaatca
      181 aatcggcaaa gtttgccatt ctgtgataag cacgaaattt ccagtcttga agttttacat
      241 cttcaaatag ttcaagacgt ttccgaaatt cagtgtcacc catttcagaa ttgagataaa
      301 caacctcata ccgtttcata ttctctttga ctatattcaa aagaaaagcc gtcttgcctg
      361 aagatttact tccagccact acaacaatat ttccgggata aagtttgcag taagaatcaa
      421 tgcctaatgg taaactgatt gaaaattcat ctgtgggtgc gtttaggaaa ttaacttctt
      481 caatttcgtt ttcaatccgg cgataaacac cgtctttatt gccgtgcttt tcaataacct
      541 tgtctttgtc ttttagtctg ctaagcgcaa cgcgcaccgc agtcttatct tcttttgtaa
      601 caacttgtaa cactgtataa cagtcagtaa cagaaaagta accatttgta acagatatga
      661 agtctaatac ttccttcgcc aagttccttt ctcttttatt ggcacgttga aaagcggact
      721 caatttttgc ctgaatccat ttactatcag gattttcacc ccatgaaaat ataagttttt
      781 caaggacttg ttcaatttct tctttcggca taccaccttt gacaaggcaa ttactgacat
      841 gaaaaaggtc attgtcccgg cggccattct caaacatttt agaggcactg acttcagttt
      901 tgacttcacc cctatatata gaattattta ttatattatt aaatgcgctt agaagcgcat
      961 taggcagcgg cggtggtgtg atctctgcta aagataaacc gtcaagccat tcataaccct
     1021 taccattgcc attcattgaa ggcgcggcaa tgatgtagcc gccttctccg cgtatatcaa
     1081 caccgggcat tatggccgct tgaacagtca agccggaatc tgtgggatat tggaaatata
     1141 agtgccagcc cccgcgcggt gttcttgctg tgggaatttc caaagaatcg ggcaagagtt
     1201 cctgaacagt gtcaaagccc ttctgtgtat cgcaatcaac aacaaacaag cctgaaattt
     1261 gccctgtgac aattccaatc atggcattag gccatttact ccaccatgct tttatttcgt
     1321 ctgatgaggc ctttctgtgc tgaaaatcag tccattggaa ataaggcttc ttattccttt
     1381 gtaacgggat aatagaataa ttatttttcc cggcgtaatt caaagccgct tgcattgtct
     1441 tcatgtccga tgattcccct atgatttaat tcatcttcaa gaatttttat ttctttctct
     1501 gtgatctgaa ttaattgccg cttcagaaaa attttcctct tgatgatttc tttttcggtc
     1561 attgagcctc attctgtttt gcaaagtggg cttgaactgt ttgtaagtga tacctaagat
     1621 atttaccgac tctgatacag gggatagcat tcggcccctt tcgtcttacc ggggcataaa
     1681 ggaaactttt tttaattttt aatttgtgac ataactcatc accagttaat aactcttcac
     1741 cggtgacttc tttaacgttt aatgattctt gatcttgcat tttcaaaacc tccaaaataa
     1801 attttgtaaa acaaaaaagc cgtggtcaac caaccacggc ctaagtaaat catgaaaaac
     1861 taaaataata ttcccgaaag tttttaaatt tttatgggaa taactaagga ctttgtttta
     1921 ttgttttatt cttgtcgaga gccttctttt tatcctcgca ataattacaa aaaccatctt
     1981 tgtttatatc gtgccaatga gcgtatcttt tcccgcagcc gtcgcattta tatcgcttcc
     2041 gaaaagttaa ctctttgtaa taataagttt ccgtcagcgt ttttccacca atacccaatt
     2101 tgcggcctat cgtctcaaat agaaaaccat caataggtgt agtgggatca tcttgtttta
     2161 ttttttctat catatcgtaa accttaaatc ccttttcccg ctcttgtctt tttgtttcaa
     2221 gatgcattcc cttcgggtgt ggtcgtccga aaacatcatc ccaacttttg gctttgtatt
     2281 gccgcacttt tcgataagct gtaatataag ccatttcaca ccaacgtgga ataggtaatg
     2341 agttcattga acataaaaaa attgcttcga gaatcgccgc gctattaccg gccttatgcg
     2401 tattatatag ttctttaagt tcctgcgcac ctagccatct aaacaacggc cctctacctc
     2461 gtgtattctt tgccttccat tcgttttgcg ctttccatgc atcatcaaac gaccatgcgt
     2521 aaagctgttt taaatcgtcg tatccgggcc cctcattcgg taaatgatat atatggtcat
     2581 ctaaattagg agagtgaata tttctgacat ttaattttga cattgcctat ccctcgcaat
     2641 tatcccggtg tagtttgaag gggcaagcgt cgggatattg ccgctttgtc gggagctacc
     2701 ctagcccctt catctatcta ggaattaata tccggccttc tatttcaaac catcttcaag
     2761 gacggtataa gtaatctgtt cattggtaac atcatgtatc tgaattaaaa aatctttgaa
     2821 cctgatttgt tgtgcatttg cttgatatgt cagattctga aaaaatgctg gccttgcaag
     2881 atcattgtaa gaatattctt tatatgttgt gtttaatgat acattatttt tacctgcaaa
     2941 aataagttca catgatattg ccgacggtgt gccacgaaca catgaatgtg aataattaag
     3001 cttgctgggg gtaattatga tagaatcgga taagtatagc atttcataat catagctata
     3061 caatacatta ttaataatac ttccatcatc caatactaaa atgccccagg tgtaagaacc
     3121 atcagaacct cttgttacat aatgtgtttt atcgttaaaa ataacagttc cgtgaattat
     3181 atattggtga ttatccttac tatgaacgat actaagatta gaagccctgt attttgcagc
     3241 agcagaaata tcttgaagag aaataaatgt tttttgaaag aaatcacatt tttgaacttt
     3301 tataatttct tgacctataa atgctgttct tttttcacct aacgtatagt tttttatgta
     3361 accacctgaa gttggattaa tgggagaaaa ttttgtattg atccattgcc cgcgcacaca
     3421 accttgaatg aagaataaac aaattagaat agaaaataat gcaatctttt tcatacttcc
     3481 ccctaattta gaattttgat caagaatctt gtgaccaaat tgtgaccaaa tttacaaaaa
     3541 acgctataaa acaccataga ggcacatcta aaatgtttca agatttcttt atgttatttt
     3601 atggtgtttt acgtaagtta ttgaaataac acgctgaaaa tccgagtgtc ggcagttcaa
     3661 ttctgccctg acccaccagt aaaatcaagg ggttgcaagc cgtaaaaccg gtttgtgacc
     3721 tcttttttta gggcactgtg ataaaattgt acattcattt ttctggtttg atcatttcag
     3781 ccagaatcgc ttcctgcgtt tccgtgtctt cgggtatagc atcaattttt ttcaggatag
     3841 gatcatcggc cctaaacata tttgtggttt ccgtctcgtc atcaaaaaca agcacaaccg
     3901 atccattcag caaggaggct tttgcctgct tgaattttgt ctccatggaa gcatcaacag
     3961 cgccatagtc cgtcccggct ctggatataa attcttctat gacgcctctt aaggctctcg
     4021 cactgagttt attgactggt ataatatgga tcatttcttc tcaacaccgt tcagtcatta
     4081 aaatgataat gctccaccgt tgattccagc aggtttatga aataattgac atcattcagg
     4141 caaagattac acttttcatg ttcaattaac ttaacatgtt ccctgaaaaa tctgttaaca
     4201 aatattttat attttgtctg cgccacttcg ggaatcaaag gatatccgga aatctgcatc
     4261 cggtcaatgc cccgacggtt ctgcttctcc gaaaaatctg gcatattcat attgccagtg
     4321 gttcgtccaa catcatgcga cgtgcccatg tcggtgaggc cttgctttgc attcataaaa
     4381 catggtagtg attgacacag cattccatca tcaaatgatg atctgtgtta attgttttgt
     4441 tttgaaaaaa atttgaaagt tcaacattat gggccttcat gtcgtctttt ccaatcgtct
     4501 tgaaattctg aaggatatcc tccttgagga cctttcccgt attcctgccg atccgtttga
     4561 agcgcaacat gtcgttgtgc cgggaacggc gatatcgcgc tatcttcaac tggccattgc
     4621 tgacgaaaaa ggcatctgcg ccaatgtggc gttcgattac cccggcagct ggctgtggaa
     4681 actggcaagg cttgtcgatg acaaggtgcc ggaacggtct gccgttgatc ctgaaatcat
     4741 gacgtggatg atcctccgaa tcctcggcga caaccggtta gccgcctacc cgcgcctggc
     4801 cgggttcatt caaaaagcgg atgatctgat gctttttgaa ttggcccggt ccgtggcgca
     4861 tgtgtttgat cattacgcga cgtatcgtcc ggattggctg gtggcatgga gtgaaggaaa
     4921 aagaataaag gatttccggg aggagtgcgc ggatgaagcg aaggtttacg aggaatggca
     4981 gatggaaatc tggcgtaccg tcacacaaga gctcaagctg ggcagcaccc acccgctgaa
     5041 aatatttctt gataaaatca tgacgggcaa aacaccggga gcagcatcgg acctgcccgg
     5101 cagggcggcg atttttgcgc tgcctgtcat cccgccgatt taccttcaga ccatccgcag
     5161 gctttcggag gtcatggaca ttacctttta cctgctcaat ccctgccggg aatactggtt
     5221 tgatatcgta ccgcccaaac agcttgccta tcttaaaaag atcggacggg acgcgcacac
     5281 ggaggtcggc aatcctctgc tggctgactg gggccggtca acgcaatcgg ccattgatct
     5341 gatatatgat gacgcgtcgt cagcaaacac actggagacg agtcgtttta tcgagccgga
     5401 gggtcaggcg ctgctcgcgc aactgcagag gtccattttc aacatgcagg agctggatcc
     5461 cggcagcgtg cagttgccgc acaacgaccg gagtgtggaa atccactgtt gtcacggcac
     5521 cgtacgggaa ctggaggttc tgcacgacag gctgctgtca ttgttttgtg cagaccagac
     5581 actgagcgcg gatgatgtcg tcgtgctcac gccggatatt gatgaactgg ccccggccgt
     5641 cgaggcggtt ttcgggaccg tgcccaagga tcgtttcatt ccctataccg tatccggcag
     5701 ggcaatggcc ggaaccaatc cctacctgaa catgcttatg ggactgcttg atctgacggc
     5761 ctcgcggatg ccggccagcc ttgtctttga tcttctgagc cagccgctgg tcgccagccg
     5821 cttcggggtg gacaatgacc gtctcaacag catccggagc tggctgaacg gttccgggat
     5881 cttctggggg attgacggcg aacacaggcg ggaactcggc ctggcggacg aagacaggca
     5941 tacattccgc agaggcctgg attcgatgct gctcggggtt gccattccgt gccttgatga
     6001 accgattgcc ggctattttc cttatgagga cttggaggga tcgcgggcgg agacactggg
     6061 tcaattatgg ctttttgtgg aaaggctggc attttggaaa aaccggctga aaaatccgat
     6121 accggccaat gattggcagg accttctcaa ccggatgctc gccgatttta ccgaatgcgg
     6181 aagagatgcc cagggggcgt atgaccaact tgtcggcgcc attgctgaac tggcagataa
     6241 ctggcgtgcg gcaaacctga caaaggcaat ttccagccgt gtgatcaagg ccgcgctggc
     6301 cgatgcggac gttagaagac gcggggcggt gccgtccgga acggttacct ttgcctcgct
     6361 gtcggcgatg cgcggcatcc ggtaccgggt ggtttgtctt gtgggcatga atgacggttc
     6421 gttcccaggg cgcgcatatt ctccggaatt cgatcttatc ccgaaggcag aaccaagacg
     6481 cggcgaccgg caaagaaatc ttgaggagcg gggcattttc ctggacgccc ttctggctgc
     6541 cggcgagatt ctgcatattt cctacaccgg ccgcgaccag cgcgacaacg ccataatgcc
     6601 gccgtccgtc ctggtcgcga aacttctgga ttacctcagc ctggcgatgg caccggaaaa
     6661 cgccacagcc aacgatctgg caacagcccg caggcagctg acggtggatc acccgttgca
     6721 gcctttttcg aggcggtatt ttgatcattc cgatccgcgg cttttcagct atgtcggtca
     6781 gtatgcaacg gccttgaatc gtggcagcaa gtcgacaccg gtgaaaaaga aagcggattt
     6841 aagaagtgat gagcaagagg aggatatgcc tgatccgaag acacctttct tcaacggtta
     6901 tggggcgcct cccaaaagcc cggatacgga gtcgacaacg ttaaccgtgg acgacctgtc
     6961 ctacttcctg atgaatccga gcagattttt cctgcgcaga cagctccgga tacagcttcc
     7021 acaggaagag gatgttatcg ccgatgaaga accgctcctc tttgaatttt cagaggaacg
     7081 tgatgcggcg gcgatgatta tcgaggcatg ccggaaacag aagcgcgtca tcacaattca
     7141 ggatgccctg gctatcctgc aggcttcccc gagttcgcct ccgggaagtg cctgcgaggc
     7201 cgccctgtca ggcatctggc cgaaagtgtc gttgcttgcc gaaaggttgc tttcggcaac
     7261 aaccggggaa aaattgcagc cggtttattc cttgctatcc attgatgttg aaggagaaac
     7321 ctggcaactc aatgccgatc tgatggaact ccgtctttgc ggccttgtct tttaccggtg
     7381 cgacgatctc agggatttcg accatctgaa tgcctgggtc cggcatctgg ttttatgcgt
     7441 ctgccggccc gagggtgtct tgccgcagac caggcacgtt gcgcttgacg gcgatttaat
     7501 cttcggggat attgcctggg aacaggccgc aaagcatctc ggcaacctgc tggcgctcta
     7561 cagagacgga ctgttcgagc cagtgccctt tttcagaaaa tcagcatgga aattcgtgca
     7621 aaaggatatg aacgcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     7741 ccctttttca gaaaatcagc atggaaattc gtgcaaaagg atatgaacgc ggcaaagagc
     7801 aagtggtccg gagggttcaa tgccggcggt aaagcggaga gtgctgatcc ctggcatgct
     7861 ctggcatggc ggggactgga ggcggacgat gttctgaatg aacgctttaa ggaaattgca
     7921 cactgtgttt ttgcgcccat cctcgccaat gaaaaattag aaaaaattgt caccgaagaa
     7981 gaaaaagggg aaaagcgtgg ccggaaaaaa taaaaaggtg gaacctctgg atgttttcac
     8041 atgcgatctt aacgggatcg ccaacatcga ggcatcggca ggaacgggaa agacgtgggc
     8101 catctgcgga ctttatgtgc gcctgattgc cgagatggat ctcccggtgg atcagatcct
     8161 cgtggtgacc ttcaccaatg cggccactgc cgaactgcgc agccgtatcc gcgacaggat
     8221 ggtggaagtg cagcattgcc tcgatactgg ccgctgcctg tctccggacc cgtttgccgc
     8281 aggttttctc agcagaatgg accggctcgg gatcagggcc aaggccggag aaaatttgaa
     8341 aaatgccttg ttgtccttcg aggaatccgc gatctttacc attcattcct tctgtcagcg
     8401 caccctcggt gagcttcctt tctcggcagt ccagccgttc aacgtcgagg tggaggcgga
     8461 cgattcggag atcatccggg aaacggcggc ggattactgg cggtgcatga cgcaacacta
     8521 cacggcggat gaaatcaacg cgctgctgca aaataagata aacccgtcat ggattgccgg
     8581 tgaaatacgg cgcctgctta acaggccgct ggcaaagctg gtcatgcccg aaacaaatcc
     8641 ctcagaaaat ccggcgtcac gaagggcggt ctggattaaa agattcattt tgaaaacggc
     8701 gccccgcgcc atccgcaaaa ccaagcagga tcgccatgtg atttcctaca atgacatgct
     8761 ttataatttg catgacgttc tcaccttggg acaattccca tggctggccg gagtattgag
     8821 aaaacgcttt ccggtggccc tgatcgacga attccaggat accgacccgc tgcagcttga
     8881 aattttccgc gccatttact ttgacaaagg aacccttttc atggtgggcg atccgaaaca
     8941 agcgatctat tctttccgca acgccgacct gaacacatac ctcgccgctc ggcgtcaggc
     9001 ccagcgtcaa tggaccatga tccataatca gcgatcggaa gaaaagctga tctttgccat
     9061 caatgcggta ttcggcgcaa acaagaacgt ctttggcgat ggtgaaaaga ttccgtaccg
     9121 cgacgtcagg aggggcacca agcccctgaa ggaacttcag ggggatcctg aaccgcgcga
     9181 acctttttcc gtctggcttt tcaagaccgg gtcgaaagca gaacccctta aagcggggga
     9241 cgcccggtcg cttgcactcg ctgcaacggc aaaggaaatg gcccgactga tcgacggagg
     9301 aatgcggggc accatccaga taggcgatga gaacttgcag ccgcgtgata tcgctgttct
     9361 cgtgcgcaca aaggcgcagg gaagacaggt taaagaggca cttgcggatt atggcatcgg
     9421 cagtgtggat ctgaccgaac gcagtgtttt tgcaaccgtt gaagctgaag aaatgaagac
     9481 cgtcctgcgg gcgatcgccg agccatctca agccgggctt ttgcgggcgg cgttgtccac
     9541 gatgctgctt ggattcggca gcagggagct ggccgatctg aatgccgatg aggccgaatt
     9601 taaccggatt gtcgaacggg tgaatgaata ccggcaggca tggctcggca gcggtttcgg
     9661 cgccatgtgg cgcaggctga ttgcacagga gagggttatc gaacggcttt tgccgctgaa
     9721 gaacggggag agaaaaataa ccaacctgat gcacctgatg gaacttgcaa caggtgcaga
     9781 ggagacgcac cgcggcattg aagcgctttt gcgctggctg agtgaaaagc gttccgccgg
     9841 ggacgaggag gatgtggcgc agctgaggct ggaaagcgac gaaaatcttg ttcaggtcgt
     9901 caccaaacac aaggccaaag gcctggaatg gccgattgtc ttttgcccgt tcgtctggag
     9961 cgaccgcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ctgccgggtg
    10081 tggcctggaa aatatccagt tactcatcgc ttaccgcctc ctcccttgaa ggaggaaggc
    10141 aacacgaacc ggcgacacag gattatgacg tcatccccct tgcgattgaa gatcatgatc
    10201 cgcctcaagt cctccgggca aagctttcgg gcgatatcat cgattttccg gccggtacca
    10261 caacaggaca ttgtctgcat gccgttttcg aaaggattga ctttcaggac gaaagcggat
    10321 ggaaagacat catcaccaac gtgctggcga attatccgcc ggtggatgcc ttagaaaagc
    10381 gcgatgatca ggtcagccgg attcacggta tgctgaaaga tgtcatgagc acccctctgc
    10441 caaccgggtt taaactcggc gacgttgcca gggcaaaaag attttccgaa ttcgagttcg
    10501 cctattcggt acgtcacctt gatcacgcaa aacttaagaa tcttctcgac aaacacggct
    10561 gccggattcc cccgctggat tttcccgatc tcgaaggtta cctgcggggt ttcattgatc
    10621 ttgtctttga acacgcgggc aaatggtacg tactggattg gaaatcgaac aagctgggca
    10681 tcagtgccgc ggagtattcc cggtcgtccg tcgatgaggc aatgttcaat catgcctatg
    10741 aatttcaggg cctgatctat cttatcgctc ttcatcgtta cttgaaactg cgtatcagca
    10801 aatatgatta cagccggcac atgggaggca ttctgtatct ctttgtccgt ggcgttcgtc
    10861 cggcatggcc ttcggcaggt atctggcata aactgcccga gccggcgctg atcgaatcac
    10921 tggatgccct gttctcttca gaaaaccgga ggaccgtata gcgttgatga gaaaacatga
    10981 aaacatacct gcggccgatt atgcatcgcc cctgtccagg ggacttgcgg attttctgaa
    11041 gcgcctttct ttgacccttg gtgctacgga agaggcggcg cgcacagcgg gcgatgccgc
    11101 cagggcgtgc gctcaagccc tggccgaagg cgatgtctgc gcgaatttct ctctgctggc
    11161 cgcagcggaa ggcaagccgg cacaggatgt acgaaagctg ctggatgctt ctttggtggc
    11221 ctcatccgcc ggggcggata aaaaattgcc cctcgtgtat gacgatgaag gacaaatcta
    11281 cctgtaccgg tactttgcct atgagcggaa actggccgat gcaattatca ggcggttacc
    11341 ggggtccgcg gcgcggacag tcagcgccga atcacggcaa tttctcgaaa aacggtttgc
    11401 ggaaaatgca agaaaactat ccggccggcc ggactggcag aaaattgcgg tagaaatggc
    11461 attcacgaac cgcctgacga tcatcagcgg aggacccggt accggcaaaa caaccatcgt
    11521 aacagccctc atcgcggcac tggcgataaa tgatcatccc ccgcgaatcg cgcttgtcgc
    11581 gccgaccggc aaagcggcgg caagaatgga agagtcgatg cgcagtcaga tgagcacact
    11641 tgacgcggag ttaaggaaac gcatgcccga gcgggcctcc accattcata tgctgctggg
    11701 agccagcccg gctggcaata cgttccgctt taacgaaaac aaccccctgc cctatgattt
    11761 gattgtcgtc gatgaagcat ccatgattga tttatccttg gcctcaaggc tgttttccgc
    11821 actgcatccg gatactggaa ttgttttgct gggggataag gatcagttgg cggcggtcga
    11881 agccggagcg gtattcgcag aactggcgca gcagacatcg gttcctgaca atatggacaa
    11941 gctttcgaca ggaagcggca caccacctct caacgctcaa ccaaacgaga aagggccgtt
    12001 gtccagttgt gttgtctggc tcaaggagaa ttaccggttc agcgcagcgt cgcccatagc
    12061 taaagtggcg ggccttgtcg ttaacggtga gggggaacaa ctcatcgaat ggcttcaaaa
    12121 tgaaaaaggg gataacatca agtgggaagc ctttgatgaa ggactgccgt cgcgtgttgt
    12181 aggggctctt gtcaaggggt ttgaaccgtt tgttgaggcg gtaaatcagg gcgatccgga
    12241 gaaggtgctt gccgcttacg aaaaattctg cgtcctttgc gtcatccgta acggcagacg
    12301 gggtgtcaac ggtatcaatg acgtcatagc gcggcagtta cgcctgaaga tatcgggaga
    12361 aggacctggt gaggggcctt ggtatgacgg tcgtccgatt atgattacgg agaatgatta
    12421 tggccttggg gtattcaacg gcgacatcgg tgtggccctc aagacgaaag acggtcatcg
    12481 tcgcgtctgg ttcatggaca aagacggagg gttgcgtttt gtgcctcctt cttccttgcc
    12541 ggctcatcaa acggctttcg ccatgacggt gcataaagcc caagggtcag aattcgaaaa
    12601 tgtctcgctt attctgcccg agcaggacac ccccgtgttg accagggaac ttatctatac
    12661 ggcggttacc cgcgcgaaga agactcttct tatatacggc agcgcggaca ttctgaagct
    12721 ggccgtaggc cgtccaacat cgcgtcgcag cggcctggcg aaaaaaatta gtacgccacc
    12781 atgaccgccg acacggaccg aatccggcaa tctttcaacg cccccccctc tgtagaacac
    12841 aacccaagcc gttttttcaa accccggcgc gtaaatattt ttggagagac ggctgagagt
    12901 ttttcttcgg ctaatgttca ggaaattttt ttatgaaggg aaattgtact ggcgcagcag
    12961 atcggcaaac tgatccggtg caacaggctt gctgaaataa aatccctgca tttcgtcgca
    13021 gattttctcc cgcaggaaat gatcctgttc cagtgtctcc acaccttcgg caacaatatt
    13081 aaggctcagc gtcttgccca tggcaatgat ggcttcaatg atcgccttgt cgtcggaatc
    13141 atgaggaaga ttacggatga acgatctgtc caccttgagg gtatcgatag ggagatgctt
    13201 gatctgggaa agggatgaat agccggtgcc gaaatcatca agggcaaggc gcacacccat
    13261 ctcctttatt ttggtcagca caccgatcag gcgttcgggt ttgaacatga tcatgctttc
    13321 cgtgatttcc agctccagaa gattcggcga cataccggat tcgtccaaag catttttgat
    13381 atcttctaga gccttatcat ccatcaactg acgaagcgat aaattgaccg ccacccggat
    13441 gggcggcagg ccctggcgct gccaggcgac gttctgtacg caggcggttt ttaagaccca
    13501 ccggccgatg ggcacgatga tgccggtttc ttcggcgacc ggaataaatt gggtcggcgt
    13561 gatggacccc agctgcgggc tgttccagcg cagcagggct tccacacccg tgatcgtgcc
    13621 ggatttgaag tccatcttgg cctgataatc cagatacaat tcgtggcgtt ccagcgcccg
    13681 gcgcagattg gtttcgatgg aaaaacgctc gtaggactgc gactgaatgt tctgtgaata
    13741 aaactggtag ttgttcttcc cttcttcctt ggcaaagtac atggccatat ccgctttctt
    13801 catcagggtc tgttcatcct ctccatccgt agggtacaca ctgatgccga tgctcgatgt
    13861 gacgcggcat tcttcaccca gcagaaccat cggtttcatg acggtgccga ggattttctg
    13921 ggcgacgatg gcgacgttgg ccaggtcggt tacatcctcg atgaggatga tgaattcatc
    13981 gcctcccaga cgtccgacaa catcttcttt atccttgggg cgtccgatca cgtcagcggc
    14041 gcgcagggtt tgccgaaatc gtttggccat ctctttcaat agtctgtcgc cggcatcatg
    14101 ccccatggtg tcattgatga ttttgaaccg gtcgagatca ataaagagaa gcgccagctg
    14161 acgcttgttg cgtttggcgg acttaatggc ctgattcagc aaatggttga acatcatgcg
    14221 gttgggcaac tgggtcagat tatcgtgtgt tgccaggtat tggatctgcg cctcggcgcg
    14281 cttacgttcg gtcacatcgc ggctttcgcc caggatggat aaaggtttcc catttttatc
    14341 ctgaatgaaa cttaacgtgg tatcgagcga tatgagacga ccgtccttgc agcgaaattc
    14401 aacttccaag gaacgcttat agtcgggcgg gggggggaca atctccgcct tggccatttc
    14461 ctctgaaaac atttccagcg ctttttggaa agattcttcg gtgagaagtt tatccaggga
    14521 gatctcttta atctcggtaa acgtgtatcc tgatgccttc tccgaagacg ggctgaaata
    14581 ttgaaacttc aagttcagat cgaagagcca cacctggtcg gtcatatgat ccgccagaag
    14641 acggtattgt tcctcggcac gcttgcgctc ggaaatatcc cggctgacgc ccagaacccc
    14701 ggtgatgtat tgttccttgt ctctgaccag ggaaaacttg gtttccgtcc acacgggatt
    14761 tccgtctttc cggttgattt ccaggagcag ggttctctca ggggaatcct ggcttttgga
    14821 tttgcccatc tccatcattt cggacaacgt ttccatcgcg gtgtccaggg aggtgggcgc
    14881 aaaggaatcg gcaagattct gtttcattac gtcttccggt tcgtagcctc tcagggtttt
    14941 gaccgacgga ctgacatagg tgtagatcag attcatatcc accacgaaaa taacatcttt
    15001 gatgttgtcc gtcagccgct cgatggtcgt aagggcattg gttatgctgg cgcggagtcc
    15061 gttgatgtat ccgccgatca tggcaaacca cggcagaata atggccagga cgataatgtt
    15121 caataaatcc agtttgggat tgatcaattc cgggtgcagc ttgtaaacaa gaaaaataac
    15181 agccgaatag ttaaccaacg cgtataacga caacagcagg aactgccgga cgcgcaaccg
    15241 gaaaaatccg aacacgagca caaccatgta aacgagaagg cacgcgcctc tggcctcgta
    15301 ggcatagtac atgaccacca taatccagaa ggtggcaatc accatttgaa gaagagtcag
    15361 actcggatct ttggcttttt tattgaatcc ggttctgaac acggcataca gaagaatatt
    15421 gacaacaata tttgcggtga ctccggcaac caggatggat aacggcactt ttgtcagtcc
    15481 cagtaaataa ctggcaatgg taatggcgtt ccacatcaca tacgcgccaa aagcgatgag
    15541 gaagcgcttg attcttaaag tctgattgta gtcattttca ggaaagatat tgatcatgaa
    15601 tattttttta ttaccgcaac atctcccttt ctggattgaa acaaatatac tcaaaaaaag
    15661 ttgttaagta aatcatttaa tagacccgga ttgcctcgcc ctccctcggc caatcgggat
    15721 tgcagggggc ccatcgcatt tccggtgatc aacagacgga tcacaaacga cgcctgttgt
    15781 tgagcattgc taaattccag gctgatcatc attgacatat tcagaacttg gtaatataaa
    15841 tgcgcatcaa aaaaattttc aggggtgtga acatcggtgc atgccggtcc ggcacgcgac
    15901 ttgcgaacaa ccagggggcg ttgaaacgga ttgcagtaag acactctttt tttgaaataa
    15961 tccggacagt cggcaaacac aacgaaccgg cttataaagg agaatatata tcttcagtat
    16021 aattcccaaa gacgatcgca agcaggcgtt gagaatcaaa agatttttca tcgccttctc
    16081 atcgtacgtc atgatttgtt ctctggtgat cctcggctat attctgggat tttcccccgt
    16141 tcctttgcgc gtactggttg tttcccagct ggggattctg gcctgtaata ttatattata
    16201 tgtaatgttc aggaccggat tgaacaaaag attcaaggat cccagtctga cgcttctcca
    16261 gatcgtaatc gccacgtgct ggcttttgga ggttttgtat tacgccgatt ccgttagaag
    16321 cgcggtgttg atggactttc tgattatttt tattctgggc cttttcaagt ttaatgtcag
    16381 ggaatttctt tttctctcgt ttttcaccgt tgccggctac accggcgtta ttttgctgct
    16441 ttatatcaat cgtcccgaat cccttaatta taaggtagaa atattgaata ttgccatcct
    16501 ggcaactgtt ttgccctggt tttcactggt cggcggatac atcacccggc tcagaaaaaa
    16561 ggtggccctc tctcttgcca ccgtcaaaga aagcgaaaaa aaatatatcg agctgagcat
    16621 cattgacgat ctgacgcaac ttttcaattc cagacatttt tacgctcaac tggaaaagga
    16681 aattgaaaga gccaatcgtt acaaccagcc gttgaccctg ctgatgctgg atctcgataa
    16741 attcaagaat ttcaacgaca cctatgggca ccttgagggc gatcaggtgt tatccaggat
    16801 cggtcatgtc atcaaacgat gcctgcgcga ttccgattcc gcctatcggt acggcggcga
    16861 agaattcgta attctactgc cgatgacaaa agcggaggct ggaataacaa cagcaacaag
    16921 aatccaggaa acgcttagaa aaacttcgtt ttcccccgtg tcggatcagg aaatttttat
    16981 gacagtaagc gtcggtctct cgcagtacat ccccagagag gatatgaaag tctttgttcg
    17041 ccgggtggac aagatcatgt accaggcaaa acatcagggc agagacagaa tttgcacagg
    17101 ctagagaatc agaggtcgcc ccttaaaagg cgcgtatcaa gagaaaaaat ccctatttta
    17161 ttctttaaaa aaattggaat cgtgctatta taattacgaa taatggttga cggtgtaatg
    17221 ctcttgatga agaggacttt cgaaaagcca tcaacgtgtg acgaaaataa ctttttgaag
    17281 aaaggaatcg cacaatgact tacaaagaac tcttggagaa gcagattgga agggaatgta
    17341 cgtttaccgc aagccatacc aagtgcgtaa ttaatttcgg agcggcagaa ccttttgcca
    17401 gcgagggaat catcgagaat gttaccgatg actatgtcat tatcaaaagt caaagttcaa
    17461 ctatgattta tcttctggtc aatacctcca tatcgtatta acacaaatca caaacggaca
    17521 aaaatcagat accggcggta atttcccccg ttctattttt accggttttg ccatgtttcc
    17581 cgaatatgac tttcaccgtc gcagggcaaa aacgcggttg atgtttttct tgtataaaaa
    17641 ataattgatg gtatggtttt catgattttc ggagttctgc ggaccggggt gataatgttt
    17701 tttcttcgct gcatactgat atgcttcgcg gcctgtacgc ttatgacatc atttgcatac
    17761 ggtaatgttg ttcaggaatc ggatgctcag gtatggaaag tcggccggca ccggtggacg
    17821 attcatgacg aataccgata cggcatctgg atagaagaaa gcgttacgga ggattttttc
    17881 attcgccata atattcgcgt ggactgtgcc gacgttccct atgccatgcg gtggatctat
    17941 gcacgcatcc atcatctccc ggcagccgca agtacagttg ataaccggtt catcggacac
    18001 tggtccaaag actggagcca tctaccgaca aatccgcact ggcataaaga cagacgtttc
    18061 cgggcggcat taatgagcgt attgtcatca acctccacac agaccctgcc gtccgatacg
    18121 tatccgataa aaatcggccg gcactccgta acaccgggaa ccgttttcct ttttgccaga
    18181 gatcatgccg gtattgtgag ccatgtcgtc atggacggga gcaccaccca tccggtccaa
    18241 acctttgaag ccagttcacc ggcaaggctt cagggattaa ggctcaagga ttttctcttg
    18301 cccaatccga atgccgatta tatttccggc ttactgaagt tccgctggcc ggtctcggac
    18361 ggcaacacgt ggcggtacct tccgctggag gagcagccgt tctattccga tgaacagtac
    18421 ctgccggctt tcacgaaagg atattccaat tatctcgaag cggtggaaaa gcgaatcaac
    18481 ccggcagtct atgagcccgg cgaaaaggcg gaaaaaataa tgatgacgct ctatcgccgg
    18541 ttaaatgaac gtgtcccgat tgttcttaaa ggttatataa aatgtcatgg catagaatgt
    18601 ccggaaggct ctcttttgtg ggaaatttac agcacgtaca atcgggatga ctttatcggt
    18661 tttctgttgc actaccttga acaaattatt gccaggaatc acctggacag aaatgacgtg
    18721 cttcataaaa tggagggcat tcgcctgcaa attactcccg gccgctttgt gactttaaga
    18781 cacgtgctgg aaaacttcaa atggattgct tccgatcccg aagccggtat tgaagcccga
    18841 tggggactgg ataaatgcag caccattgcg acccgtctcc gaaccgcaga ggagtctatc
    18901 gtgtttattc agaacaaata cggcgagacg gatcctctgt ttgccgaaca gtccatcctg
    18961 aaacaacaga aaatcgttga cgaaatgacc agtgaaagcc tggaaaacaa ctgcatgaaa
    19021 aacgcacttc gataatgatt gtctcgcccg ctccaaaaat ctattttact gcgagcgtcc
    19081 cggcattacc gcgacctatt cccattcgat ggtgctgggc ggtttggaac tgatgtcgta
    19141 aacaacacga ttgacgccgc ggacttcgtt gataatccgg ttggatatcc gggccagaac
    19201 atcgtgcggc agcggcgccc agtcggccgt catggcgtcg tcgctggtga ccgcgcgaat
    19261 cgacaaaatg ttttcatagg tgcgctgatc gcccataatg ccgacgctct tgatcggcag
    19321 aagaacggca aaggactgcc acagtttgta atacaacttt ctggcgcgga tttcttccca
    19381 gagaatcgta tcggcttttc gcaaaatcga caggcgctgc ggcgtcacct caccgataat
    19441 ccggatggcc agacccggcc cgggaaacgg ctgccgccag acgacatcgt ccgccagacc
    19501 cagttctttt cccaaaagcc ttacttcatc cttgaataaa tgcttcagcg gttctatcag
    19561 ttgcaggctc atggttttgg gaaggccgcc gacattatga tgcgatttga tcacggcgct
    19621 gggaccgccg aacgccgaac acgattcgat taaatccgga tacagggttc cttgagccag
    19681 aaatttcaca tttttgattt tgcgtgcttc gcgattaaat acctcgatga aggtacggcc
    19741 gatgattttg cgtttccttt ccgggtccgt cacgcccttc agttttttca gaaatatttt
    19801 gccggcccgt gcaaagcgca gattgatctg caaatgtttt ttgaacatcc cgatcacgcg
    19861 ctgcgcttcc ccttctcgca agacaccgtt gtccacaaaa acacaggtca gttgtttgcc
    19921 gatcgcctta tgcaacagca cggcagcaac ggacgaatcc acgccgccgg aaagtccgag
    19981 aaggactttt tcacggccta cctgccgccg gatgtcgttt acggcttcct ggacaaagga
    20041 ctgcatggac caggtttttt tacaccggca gattccgaaa agaaaattgg ccagcatgat
    20101 tctgccatcc cgggtatgaa caacctccgg atgaaactgc aggccgtaaa aatgccgccg
    20161 aacatcttgc gccgccgcca ccgccgtatt ggccgtggtt gccgtcacgg caaatccttt
    20221 gggcagctct ccgatggaat cgccgtgact catccagcag ggagttttcc gggcaacgcc
    20281 cctgaaaata cctttttgtg ttttgaccag aagctcggca aatccgtatt cgcgtttcgg
    20341 agagctgatc accttccccc ccagagagtc gatcatgtat tgcagaccgt aacagatgcc
    20401 cagcacggga atacccagtt tgaagatttt tttatccacc cgcggcgcgc ctttgctgta
    20461 gatgcttgcc ggtccgcccg ataaaattat tccctccggt tccatctccc ggatggcggc
    20521 tatggaaatt ccgggcggtt caatctggca gtacacatga ttttccctga cccggcgggc
    20581 aataagctgg ttgtattgag atccgaaatc gatgatcaga atcacgttgt cttctccttc
    20641 tcctgttcct tggttttcat atactacgca tacgactatt tttgcctgcg ggctccggat
    20701 gccgctgatg catgatcccg cgcgccaaag gcctgcggca ccggcctgat attccgcagc
    20761 cgcctcaaaa gcggcagccg cattgccgta tgcgccgaag gtgcggttgt ctatcttttt
    20821 ttctccgttt cgtcaatgag caaagagccg gggaattgtc gtccggaccg tttccgtccc
    20881 atggcatcta tcaactgata aacagcattt cccagaattt cgggacaggc cagatatcgt
    20941 catcaaccag gatctccagg gcatccgcat actcgcgcag ttcctccatc attggcttga
    21001 ccttggcgct gagcatctcc gctttcttct gctcatcatg cagagcattg gccgcctcga
    21061 ttttcacctg aatctctttg ttcgttgcat ggatgctgtt gatcagatcg gtgattttct
    21121 tcaacagctc aatctccgcc gttacagaag cggcattccc cagcgcttgc ctggtgctgt
    21181 tgatcgcatc agccaacttt ttctgataca ccacggcagc cggaatgatc tggctcgtac
    21241 acagagtatt gagacattta acttcgattt ccaaatcctt gatgtagcgg tcaacgtgaa
    21301 tcaactgccg ggacttgagc tccggattgg agagaacctg atatttttca aaaagattca
    21361 acgccttgac cgtcgtcagg gccttcaggg cggtaggcgt ggttttttcg ttcggcaggc
    21421 ccctccgggc cgcttcgatt tcccattccc tgctgtagtt gtcgccgttg aacaataccg
    21481 ctttcgaata gctgatttct tctttcaaaa cttccagaac agcctgattg atgttcttgg
    21541 agttcttcgt tttgattttt tcagccagct catcgaggct ttccgccacg attgtattaa
    21601 tgaccatcgc cgggaaggaa atgttctgcg atgaccctac ggcacggaat tcgaatttat
    21661 cgccagtaaa ggcaaaaggc gatgtcctgt tgcggtcggt attgtctttg ctgagcgtcg
    21721 gcagtgagaa aatccccaaa tcaatcagat ccgcattggt tgccttggtc acttttcccc
    21781 tctggatatt ttccagaatc tgcgtcagtt tttcccccag aaacacggag atgatggccg
    21841 gcggcgcctc gttggcaccc aggcggt
//
LOCUS       DAONQZ010000039        21475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2753_length_21475_cov_14.768768, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000039 DAONQZ010000000
VERSION     DAONQZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21475
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_21475_cov_14.768768"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLR13_07545"
     CDS             <1..1182
                     /locus_tag="PLR13_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19228.1"
                     /translation="VFSMITVFAQYKPHAMHFSFHSRLTNSLCSFAIYLEKTFWPHDL
                     AVFYPFDAHIALLQVSGATVLLITISLIVVLLYKRCPFLFTGWFWFVITLLPVIGIIQ
                     VGKHALADRYHYLPSIGIAVMIAWGLSVLMTNDYRSKRILSVAGVAFMVIMTLLTWKQ
                     CGYWKDNPALFTHALKVTKNNYIAHDYVALSLFAEGKYEKAKDHFDKALIINPDYVET
                     YNNRGMLYAETGHYEKALVDFNRAIRMKSDYADAYYNRGLTYNRLGHHQQAFDDYSRA
                     ILLNPEHIKAYNNRGIFFFRLGDYQSAIQDFGQAITLKTDYAEAYFNRGITYAKIGKG
                     HRALEDFSNAIRIEENFTHAYGNRAAVHFKQGNHKLGCADARKACALGNCTVLKSADC
                     R"
     gene            1270..2985
                     /locus_tag="PLR13_07550"
     CDS             1270..2985
                     /locus_tag="PLR13_07550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19229.1"
                     /translation="MPPILLLCFVSLCFFGQFSNSVTVDEFVHFPAGIYILKTMDWRM
                     DHPAPPLVKCFPAVSAIFTHPEIETKLFQKYPDLYGLGYRFMEQNKDRYRSIFTYGRS
                     MIIFLGFLLGITIYFWGKELYGYKGALFALILYAFNPNILAHASLITMDIGVTLTIFL
                     SVYGLWKYFKKPNAFSIVIAGVALGLAQLSKFTALLLYPVYFFIFGTLCIISLCKRKD
                     EGIKSSVMTISKYGKDFLMIIFISIFMINLGYLFSGSFMPLSEYNLSSRLFNNLASLL
                     WSGLPVPLPDHYISGFDSQLMQSVGGNPFYIGYLMGEHSMSGWWYYYIVAFLVKNPVS
                     WLIILCLTVIAWLEIKIARNDIITFLCISMPVIFYCIYFSFFTNIPIGIRYLLPIFPF
                     LFLASGFLFHQAIIKFKRIQYLLMFLIIIYVPSSLIAFPDYLSYFNIAAGGSRNGSHW
                     LMDSNLDWGQSLPALKKYMDKNNIDKIKLGYFGRVDPEIYGIDYSLAEQKPTQGIYAI
                     SINFLVGRPYYLLRENTHELLYIDINYYDQYRYLEPSAVVGHSIYIFDLRNKFSARPS
                     GKLHY"
     gene            3076..4467
                     /locus_tag="PLR13_07555"
     CDS             3076..4467
                     /locus_tag="PLR13_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19230.1"
                     /translation="MKILLVRPPSFKMPIIIPNLGLGYLASVLRHNGHTVSLLDCAKT
                     KTTHRQYRDCLMDEQPDIVGIQMHTCDFSSARQCIEIAKTVNGKTVTIVGGPHPSGDP
                     EGTMEIPGLDYAFVGEAESGLPRLLEYTRSAGQVAIDSIDGLVHRQNGKIIKNKKGAI
                     QNPDDIPFPGWDLIDPQTYPAAPHGTFSKSLPVAPVITSRGCPFQCTYCGANVNTGRH
                     FRKRSVENIVEEIKYLQKTFGIREFHIEDDNFTLDKARVMAFCHKLKEENIKLHWACT
                     NGVRLDTLDKELLQNMESAGCYSLAAGIESGSPRIINDMKRNETLDIMIEKINLIARE
                     TKIRITGFFMIGYPEETRNDIEKTIELAMKLPLHRAQFSNFLPLPGTEIYDRLIQRKE
                     IAPDTLPWDLYQNNRVVYTPKNISPREMRRLMRKAFAAFYFRPRILWGLLKEIHSPGQ
                     FRTVVRRFFDVFR"
     gene            4609..4977
                     /gene="queD"
                     /locus_tag="PLR13_07560"
     CDS             4609..4977
                     /gene="queD"
                     /locus_tag="PLR13_07560"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQC19231.1"
                     /translation="MYEVTIIKSFSAAHLLADIGGKCETLHGHNFKVEVSVAADQLDA
                     NGLLIDFRFLKKILGEILDDIDHKHLNALAHFKGINPSAENIAKYIYDQMDEKVKTAG
                     VHMVRVKIWESENAAVSYTR"
     gene            5008..5775
                     /gene="folE2"
                     /locus_tag="PLR13_07565"
     CDS             5008..5775
                     /gene="folE2"
                     /locus_tag="PLR13_07565"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HQC19232.1"
                     /translation="MIDIQSQKDSRNIDIKKVGVKNIKYPVIVLDRAKGTQLVNATIN
                     MYVNLPHHFKGTHMSRFVEALNEFKGQINIKTFRTILEKMRQKLHAHSAHMEIEFAYF
                     LEKTAPVSKAKSLMEYRCIFAGESNAETSDFLVGVIVPVTTVCPCSREISQYGAHNQR
                     SVVTTKVRFKKFFWIEDLIRIVEDSASGEVYSLLKRVDEKYVTEKAFENPKFVEDVVR
                     SVAVKLNKDDNFTWYCIEAENFESIHNHSAYALVEKG"
     gene            5780..7858
                     /locus_tag="PLR13_07570"
     CDS             5780..7858
                     /locus_tag="PLR13_07570"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQC19233.1"
                     /translation="MPANKSKNFFNLYQHGFIRAAVCIPEVKVADPVFNAKKTIELAR
                     QAAQQKAILAVFPEMGLSAYSNEDLFHQDALLQGTLQAIADVKKASARINIVMIAGAP
                     LQVDCRLFNCAVVFYRGRILGVAVKSYLPNYREFYEARQFTPADAAFSSKIKLAGQND
                     ITFGADIIFKIANIKHFSFFIEICEDLWVPVPPSSFAAMAGATVIGNLSASNITIGKS
                     EYRQQLAANQSARCVSAYLYAAAGPGESTTDLAWDGHAMIHENGNLLAESPRFSQQAQ
                     IIYSDIDLDRLAQDRMRLTSFSKNAAAHKEKILSFRKTEFDVDVPTGKILLTREYPRF
                     PYIPADPSKRDQRCYEAYNIQVQGLAKRLKASGIPNIVIGVSGGLDSTHALIVAARTM
                     DTLKLPRKNVKAYTLPGFATSRKTYNNALKLMTALGVESNEIDIKPACRQMLKDIKHP
                     YANGKQKFDITFENVQAGQRSAHLFRLANMRNALVVGTGDLSELALGWSTYGIGDHMS
                     HYNVNASVPKTLIQHLIRWVAQTGQFSQDVSDILIDVLKTEISPELIPGKNKDHLVQK
                     TEDTIGPYELQDFNNFYISRFGYLPSKVAFLAYHAWKDRDKGLWPDNIAREKKNAYSL
                     KEIKKWLHVYLYRFFKTSQYKRSCIPNGPKVGSGGSLSPRGDYRAPSDSEAEIWLQNW
                     ATIPEEDIRS"
     gene            7855..8052
                     /locus_tag="PLR13_07575"
     CDS             7855..8052
                     /locus_tag="PLR13_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HQC19234.1"
                     /translation="MRATYKDFDATELFCPRCKKAVAVRKRLLLILQEGEKYDYTCVH
                     CGTSIGDKLVKDSAPSGLVIN"
     gene            complement(8055..8414)
                     /locus_tag="PLR13_07580"
     CDS             complement(8055..8414)
                     /locus_tag="PLR13_07580"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HQC19235.1"
                     /translation="MKKAQQSSSGEDYREKSLAMNGLVCARCGREFPESKRHLLTVHH
                     KDGNHHNNPADGSNWENLCVYCHEDVHSREVLAEYLGGEAHGKEVSLVYNDAQGTVGS
                     GLLAEKLKTALKKKSDK"
     gene            complement(8525..8938)
                     /locus_tag="PLR13_07585"
     CDS             complement(8525..8938)
                     /locus_tag="PLR13_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HQC19236.1"
                     /translation="MTLEIALLILVGILIVLAVGCIPLLVQIWRISKDLEMTLQTFNQ
                     SLPLLLKNLEEITTNVNSSSSVINKKIQNFAHPSNMSGLLLGDIISNIHHFAPLALKL
                     PIFRIMRNVVSIAKGLRVFVEVLLNKEKDETLKQG"
     gene            complement(9002..9385)
                     /locus_tag="PLR13_07590"
     CDS             complement(9002..9385)
                     /locus_tag="PLR13_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQC19237.1"
                     /translation="MTEKNGDVLKGLFIGGLIGIALGILFAPKSGKETRDDILLKTDE
                     MLARVKEEYEKALEKSRLAYEDSLNRLKGVNLSVKAKAGEAEGKISELAQQGSEAMLS
                     NKNKLKKAIDAGLKAYKEELGKEKI"
     gene            complement(9566..9781)
                     /locus_tag="PLR13_07595"
     CDS             complement(9566..9781)
                     /locus_tag="PLR13_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19238.1"
                     /translation="MKKIFAIFVAMLFVASLSLAVVGCKKAEEPAVEAPAVEAPVAPA
                     PEAAPAPAPEAAPAPAPEAAPAPAPAK"
     gene            10230..11006
                     /gene="mazG"
                     /locus_tag="PLR13_07600"
     CDS             10230..11006
                     /gene="mazG"
                     /locus_tag="PLR13_07600"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQC19239.1"
                     /translation="MESLNRLIDLIRKLRAPDGCPWDRQQKKEDIGKYMLEEAYEAID
                     ALEKKNPEALKEELGDVLFQILFICEICAESGLFSLEDVMDGVNEKMIRRHPHVFGTV
                     KVTSVQDVKDNWQQIKKKERKETGREKSLFSSIPRSLPALKRAQKITALASQSGFDWK
                     DIRSVRDKLEEEWQEFEAAVRAENRIKTEEELGDILFTMVNLSRFLRVDAETALSKTT
                     DKFLRRFAHVTEQLSSRGLKPEEATLQQMDALWDEAKKKD"
     gene            11009..11194
                     /locus_tag="PLR13_07605"
     CDS             11009..11194
                     /locus_tag="PLR13_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HQC19240.1"
                     /translation="MKDLLCVLGLVFIIEGLPYFVFPEKLKVYLAKIMTLPDSTLRFI
                     GISSMILGLILLYFGRR"
     gene            11263..12300
                     /gene="queA"
                     /locus_tag="PLR13_07610"
     CDS             11263..12300
                     /gene="queA"
                     /locus_tag="PLR13_07610"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQC19241.1"
                     /translation="MQLKDFSYDLPEELIAQHPCARRDQSRLLLMGRHKDTLADMSFF
                     QLPDLLKKGDVLVVNDSRVIPARLFGRKQTGGQLEILLLNVKKKEPTSQIWEVLIRPA
                     KRLHEGDDIRLAGHCEAKVLARISEKKWLLEFSAPDGFESYVNTFGRAPLPPYIKRMK
                     NTRPDAADLDRYQTIYARHPGSIAAPTAGLHFSDGVIQQLKDRGIQIAHVTLHVGYGT
                     FLPMTTDEVEKHVMEPEHYEISPESARIINNAERVIAVGTTSTRTLESVSNEDGYISA
                     RSGVTSLFIYPGYKFKRINGLLTNFHLPQSSLFLLVCAFAGTDLIKKAYAHAVKNRYM
                     FYSYGDCMLIL"
     gene            12326..13495
                     /gene="tgt"
                     /locus_tag="PLR13_07615"
     CDS             12326..13495
                     /gene="tgt"
                     /locus_tag="PLR13_07615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQC19242.1"
                     /translation="MRARVDPVYRSQDIKMFRFDVIKKDDKTAARMGEIKTARGIVQT
                     PVFMPVGTQGTVKSMLPEEIKNCGAQMLLSNTYHLYLRPGHETIKELGGLHRFMNWPY
                     PILTDSGGFQVYSLGTLRKITPEGVMFRSHIDGSRHFLTPQKAIEIQEALGSDIMMCL
                     DECTPYPSTPSQTEKSLNLTAKWALSCREAKTNNGQALFGIIQGGSYPDLRRQSLEKT
                     VLLNFDGYALGGVSVGEPKEIMYTITDVMAPLLPENKPRYLMGVGTPEDIVFGVSCGI
                     DMFDCVIPTRCARHGLLFTNSENVVIKNARWREDADPIDETCDCYTCRNYSRAYLRHL
                     YFAGEILAMVLNTIHNVRYYMRLMENIRLAIQENRFEAFKNAFLKDRTESKKHLT"
     gene            13515..13874
                     /gene="yajC"
                     /locus_tag="PLR13_07620"
     CDS             13515..13874
                     /gene="yajC"
                     /locus_tag="PLR13_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQC19243.1"
                     /translation="MTLTSLAYAMGGAPGGPAAGGGGDWGFIITMVIIFVIFYFLLIR
                     PQQKKQKELKAMLDNLAYGDMVMTTGGIHGKITGLTDAIVTLEIADKVRIKVARSAIG
                     AIVQKSGSAAPAAPTKS"
     gene            14011..15498
                     /gene="secD"
                     /locus_tag="PLR13_07625"
     CDS             14011..15498
                     /gene="secD"
                     /locus_tag="PLR13_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQC19244.1"
                     /translation="MFKEKIHLGLDLQGGMHLVLEIDTDKALDAMVERLSNDLKESLM
                     DSKVRFRNLEKMQPAKISMELAGADEEKGLENVLREHYPDLEIESASPREDGKFVTLR
                     INDKRAAELKKLTVEHSLETIRNRVDQFGISEPEIIPEGDDRILVQLPGIKDPERAKN
                     LIGKTALLEFKLVDDENSLDEALRGNIPEGDVIAYGLRADQSTGVRGSSPYLLKDKSL
                     MTGASLETAKVQIADRFGDVTVGLKFNSQGAVDFERITGENIRKRLAIVLDGVVHSAP
                     VIQERISGGQAQITGNFTMEEARDLAIVLRAGALPAPVNILEERTVGPSLGSDSIRQG
                     VMATMIGSLLVILFLIIYYRLSGAIADVALITNIFLVLAILAAFRATLTLPGIAGLLL
                     TVGVAVDANILIFERIREELRTGKTIRIALDTGYNRAFITIIDTHITGIVAAAFLIMF
                     GTGPIKGFAVTTIIGLLASLFTAVFVTRVIFDYITTNFNIKKLSI"
     gene            15527..16435
                     /gene="secF"
                     /locus_tag="PLR13_07630"
     CDS             15527..16435
                     /gene="secF"
                     /locus_tag="PLR13_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQC19245.1"
                     /translation="MQLIKPGINLDFVGKIKYAITFSVILIIVSIGSVIYHGGLNPGI
                     DFAGGSVIQVKFSKNISADQIRGALKSTRLQNSTIQQFGTNEFLIRTSESFSDQTVLA
                     ANVEQPLAAAFSKDFEIRRVEVVGSKVGADFKRKAVIAVVLSWIAMLIYVGFRFEFRY
                     GVGGIVALVHDVIITIGAVSIMNMEFTISILAALVFLIGFSINDTIVTLDRIRENVKL
                     NPKRQLRETINLSINQTLSRTVLTSLTVFLTVLALYLFGGAVIRDFAFALLVGVVIGT
                     YSSIYIACTIVLMFENLQISKMKRTP"
     gene            16500..16952
                     /locus_tag="PLR13_07635"
     CDS             16500..16952
                     /locus_tag="PLR13_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQC19246.1"
                     /translation="MNFVDLPGTVVIFFDVVLYAILTGFQQVGWQIIVLLLICAVVAE
                     SLEFFWTIRETPPQEHRPLKSLKATIIGAVTGAFLLTPFWAAPGAFAGFLLGGLAGIL
                     MMEIIRHCEAKIHHWTLSSVIFNTIGKNAVKGFISLFMIAFSLSNIYS"
     gene            16968..17588
                     /gene="lepB"
                     /locus_tag="PLR13_07640"
     CDS             16968..17588
                     /gene="lepB"
                     /locus_tag="PLR13_07640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQC19247.1"
                     /translation="MTEENKTKSKVKEYVESIIIAILIALFIRTFIICAYKIPSRSMV
                     PTLLVGDHILVNKFIYGVKIPLLRRTIIPVSEPKRGEVVVFIYPNDRSKDFIKRVIGV
                     AGDKIEIKNKKIFINDEEFKDSYGIFSDSLVLPQSLQPRDNFGPIIVPEKSIFVMGDN
                     RDESLDSRFWGFVNLKDVEGKAFVIYWSWNRDENNLRWNRLGQLLH"
     gene            17845..18393
                     /gene="pyrR"
                     /locus_tag="PLR13_07645"
     CDS             17845..18393
                     /gene="pyrR"
                     /locus_tag="PLR13_07645"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQC19248.1"
                     /translation="MSNTKSTKTVMDKEGIERCLTRIAYEILEKNKGMENLVLVGIRT
                     GGVYLAERLQKKMSEIEHGKIPMGILDVTLYRDDISVSNKKPRLGETKINFSLDEKIV
                     ILVDDVLFTGRTIRAAMDALIDFGRPRMIQLAVLIDRGHRELPIRADFVGKNLPSSLW
                     EAVRVDLVEKNGVDEVVIEEGT"
     gene            18417..19343
                     /locus_tag="PLR13_07650"
     CDS             18417..19343
                     /locus_tag="PLR13_07650"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQC19249.1"
                     /translation="MKFERKDILGIKDLSVDEINLILETAGSFLEVSTRKIKKVPTLR
                     GKTVINLFYEASTRTRTSFEIAGKRLSADTINISSSTSSVMKGETLIDTARNLEAMNP
                     DVIVIRHSAAGAPHLLASLVKQSIINAGDGAHEHPSQALLDMMTIKERKGNFPGLKVA
                     IIGDIEHSRVVRSNIYGLSKMGATVVVAGPATMLPRDIEQMGVKVFDKMEDAITDADV
                     VMMLRIQLERQKQNIFPSLREYSQRYCLNRNNIKLAKKDVIVMHPGPINRGVELSPDI
                     ADDPSFSVILDQVNKGVAVRMALLYLLTGGSE"
     gene            19340..20638
                     /locus_tag="PLR13_07655"
     CDS             19340..20638
                     /locus_tag="PLR13_07655"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQC19250.1"
                     /translation="MKLLLTGARVIDPAQKIDSRLDVFLENGKIARVGANLSKSTKSK
                     DAEKITKIIDLAGMIIVPGLIDMHTHLREPGFEYKETVASGAAAAVAGGFTSIACMPN
                     TNPVNDNRSITEFIKAKAAEANLANVYPIGAISRNSDGDQLTEFWDLKEAGVIALSDD
                     GKPVMNAALMRRAMEYAHSLQLPVINHCEDIHLSANGAMNESYYSTILGLNGIPAIAE
                     EVMVARDILIAEYTGTSVHIAHVSTAGSVRLIRSAKERGVNVTAETAPHFFTLTDEAL
                     QGYDTFFKVNPPLRGEKDVKAIKEGLADGTIDVIACDHAPHARTDKEVEFEYAANGIS
                     GLETSLSLSLNLIHSKILSWPELVSRMSTRPARILNLPKGTLKEGADADITVIDPLLP
                     WTVDVQAFRSRGKNSPFHGRQMQGKALMTIVGGEIKYDGR"
     gene            20625..21401
                     /gene="recO"
                     /locus_tag="PLR13_07660"
     CDS             20625..21401
                     /gene="recO"
                     /locus_tag="PLR13_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQC19251.1"
                     /translation="MTAVREHFKTTGFILRTLSYSESDLIVSFYSHDFGKLKGIAKGA
                     KRSKKRFANVFEPFSLTDIIFTRKSRDTLAFIESCDIVDHYDAIRANMEKTLIASYFI
                     DLTDHFSPEGKKNEDIFRLLQDFLVLLGREKVSDSIVRFFEIRLLKLAGFEPALDHCV
                     ICRTPVTNGNAYFFNAGRGGITCSACAKTQKYEQPISAGTLRTLLLGKDMDIDKIKLV
                     TLTDSSATESRCLLTGFISHVLGREVKSWKVLEQVRRYCT"
BASE COUNT         5749 a         5319 c         5001 g         5406 t
ORIGIN      
        1 gtcttttcca tgattaccgt ctttgcccag tacaaaccac acgcaatgca tttttctttt
       61 cattcacgac tgacaaacag tctttgctct tttgccattt atctggagaa aaccttctgg
      121 ccgcatgatc tggccgtatt ctatcctttt gatgcgcaca ttgctctctt acaggtttcg
      181 ggagcaaccg ttctgctcat cacaatcagc ctgattgtcg tcctcttata caagcgatgt
      241 ccttttcttt tcaccggatg gttttggttt gtcatcacgc ttctgccggt tatcggaatc
      301 attcaggtcg ggaaacatgc gctggccgac cgttaccatt atctgccgtc tatcggcatt
      361 gctgtcatga tcgcatgggg attgtctgtt ttaatgacga atgattacag aagcaaaaga
      421 atcctgtccg ttgccggtgt cgcctttatg gtcatcatga cattattaac atggaagcag
      481 tgcggttact ggaaagataa tcccgcttta ttcactcatg cattgaaggt tacaaagaac
      541 aattatatcg ctcacgatta tgtcgcttta tctctttttg ctgaaggcaa atacgaaaaa
      601 gcaaaggatc actttgacaa agcgctcatt atcaacccgg attatgttga aacgtacaat
      661 aacagaggga tgctatatgc agaaacaggc cactacgaaa aagctctggt ggattttaac
      721 cgcgctatca gaatgaaatc ggattacgcc gatgcttatt acaacagggg attgacttac
      781 aatcgtctcg gacatcatca acaggcattt gatgattaca gcagagcaat ccttctgaat
      841 cctgaacata taaaagccta caacaaccga ggaatttttt tctttagact tggcgactac
      901 cagtcggcaa ttcaagattt tggtcaggcg attacattaa aaacagatta tgccgaagcc
      961 tatttcaaca ggggcatcac ttacgcaaaa atcggcaaag gacatcgcgc tcttgaagac
     1021 ttcagcaacg cgattcgtat tgaagaaaac tttacccatg catacggcaa cagagccgcg
     1081 gttcacttca agcagggcaa ccataaactg ggctgtgctg atgcgcggaa agcatgtgct
     1141 ctgggaaatt gcaccgtatt aaaatccgct gattgtcgtt gatttccggt caggaggagt
     1201 attaaattga tcaactcaat gaataacatc ttatcatcct ccgctgccaa gaacatctta
     1261 agaaaaataa tgccgcccat tctgctttta tgttttgttt ctctctgttt tttcggtcag
     1321 ttttccaact ccgtcacggt ggatgaattt gttcattttc cagccggcat atatatttta
     1381 aaaacgatgg actggcggat ggatcatccg gcgcccccgc tggtaaaatg tttccctgct
     1441 gtcagcgcaa tcttcacgca tcccgaaata gaaacaaaat tattccaaaa atatccggat
     1501 ttatacggtc tgggataccg ctttatggaa cagaacaaag acagataccg gtcaatcttt
     1561 acctacggcc gcagtatgat tatttttctg ggttttctac tggggatcac aatttatttt
     1621 tgggggaaag aactttatgg atacaaaggc gccctgtttg ctctaatttt atatgctttt
     1681 aatcccaata tcctggctca cgcctctctc attaccatgg atataggtgt aacactgaca
     1741 atattcctca gcgtttatgg cctgtggaaa tattttaaaa aaccgaatgc cttttcgatt
     1801 gtcattgccg gtgttgcctt gggtctggcg caactcagta agtttacggc gttgctgctt
     1861 tacccggttt actttttcat ctttggaacc ttatgcatca tcagcctctg taaaagaaaa
     1921 gacgagggga taaaatcttc agtgatgacc atcagcaaat acggcaaaga ttttctcatg
     1981 atcattttca taagcatttt tatgattaac ctcggctatt tgttctccgg ttcttttatg
     2041 cctttatctg aatataatct ttccagccgg cttttcaata atctcgcctc attactatgg
     2101 agcggacttc cggttccgct gcctgatcat tatatatcag ggtttgattc ccagttaatg
     2161 caatctgtcg gtggaaaccc tttttatatc ggttatctga tgggagagca ctcgatgtcg
     2221 ggctggtggt attattacat tgttgctttt ctcgtcaaga atccggtctc atggctcatc
     2281 attttatgcc ttaccgtcat cgcctggctg gaaattaaaa tagcacgcaa tgatatcatt
     2341 acgtttctct gcatatcgat gccggtgatc ttctattgta tttatttctc tttttttaca
     2401 aacattccca tcggcatcag atatttgctg ccaatattcc cctttctgtt tctggcatcc
     2461 ggctttcttt ttcatcaggc catcataaaa tttaaacgca tacaatatct tctgatgttt
     2521 ctcatcatta tatatgttcc atcctcgctc attgcttttc cggattattt atcttatttt
     2581 aatatcgccg ccgggggctc ccgaaacgga agccactggc tgatggattc caacctggac
     2641 tggggacaga gcttgcctgc cttaaaaaaa tacatggaca aaaataacat tgataaaatc
     2701 aaactggggt attttggacg ggttgatcct gaaatatacg gcatcgacta ttctcttgcg
     2761 gaacaaaaac caacgcaagg aatatacgcc atcagcataa atttcctcgt gggaagacct
     2821 tattatcttt taagggaaaa cacgcacgaa ttgctgtaca tcgatatcaa ctactatgat
     2881 caatacaggt atctcgaacc gtctgccgtt gtcggtcatt cgatttacat tttcgatctg
     2941 agaaataaat tttccgcacg gccttcagga aagttacatt attgatggtt attcattatt
     3001 tactggcaac ggatatgaac accgtcaaca tcacgatgag catgaagcaa tgaatccatt
     3061 acagagttca atgatatgaa aattctgctt gtacgtccgc cgagttttaa aatgccgatc
     3121 atcattccca atctcggact gggatacctc gcttcggttc tcaggcataa cgggcacacg
     3181 gtcagtcttc tcgattgcgc gaagacaaag acaacccacc gacaatatcg ggattgtctc
     3241 atggatgaac aaccggacat cgtcggcatc cagatgcaca cctgcgattt ctcctcggcc
     3301 agacaatgta ttgaaattgc gaagactgta aacggcaaaa ccgttaccat cgtcggcggt
     3361 ccgcatccgt cgggagatcc ggaaggaacc atggagattc ccggtctgga ttatgctttc
     3421 gtgggagaag ccgaatcggg ccttccccga ttgctggaat atacccggtc tgccggtcag
     3481 gttgcaatcg attccatcga cggattggtt catcgccaaa acggaaaaat cataaaaaac
     3541 aaaaaagggg ccatccaaaa cccggatgac atcccctttc ccggttggga tttgatcgac
     3601 ccgcaaacct atccggcggc gccgcacggc acgttttcaa aaagtctgcc tgtagcgccc
     3661 gtcataacca gcagagggtg tccttttcaa tgcacctatt gcggtgcgaa cgtaaacaca
     3721 ggcagacact tccggaaaag aagtgtcgaa aacattgttg aagaaataaa gtatctgcaa
     3781 aagacattcg gcatcaggga gtttcacatc gaggatgata atttcacgct ggacaaagcg
     3841 cgtgtgatgg cattctgcca taaactcaag gaagaaaata ttaagctgca ctgggcctgc
     3901 accaacggcg tcagactgga taccctggac aaagaattgc tgcagaacat ggaaagcgcc
     3961 gggtgttatt ctttagcggc aggaatcgaa tccggttctc ccagaatcat caacgacatg
     4021 aagagaaatg aaacactcga catcatgatt gaaaaaatca atcttattgc ccgggaaaca
     4081 aagatcagaa taacgggttt ttttatgatc ggctaccctg aggaaacgag aaatgatatt
     4141 gagaaaacca tcgaacttgc tatgaaactc cctttgcaca gagcccaatt cagcaatttt
     4201 cttccccttc cgggaacaga aatatatgat cggctcatcc aaaggaaaga aattgctccc
     4261 gataccctgc cctgggatct ctaccagaat aatcgcgtcg tctatacgcc gaaaaatatt
     4321 tcgccccggg aaatgcgacg actgatgaga aaagccttcg ccgcgttcta ttttcgtccc
     4381 cgtattcttt ggggattact caaggagatc cattccccgg gccagtttcg tacagtcgtt
     4441 cgccgttttt ttgatgtttt cagatagata ttccccctga tgaaatctgc acccttcatt
     4501 tcagaggacg caacgatgct tcatattatc ggttgtcaat aattaatgat tcaacatctc
     4561 ttgaaaatat gataataatt ctactttaag aaacggagca catgaccaat gtatgaagtt
     4621 acgataataa aatcattttc cgccgcacat ctgctggccg acatcggcgg caaatgcgaa
     4681 acattgcacg gtcacaattt caaggttgaa gtatccgtgg cagccgatca actcgacgcc
     4741 aacggtcttt tgatcgattt ccggttttta aaaaaaatcc tgggggaaat tcttgacgat
     4801 atcgaccaca aacacctcaa cgcgctggcc cattttaaag gcatcaatcc ctcggcggaa
     4861 aacatagcaa aatatattta cgatcagatg gatgaaaaag tcaaaacggc cggtgtgcac
     4921 atggttcggg tcaaaatctg ggaatcggaa aacgccgccg tgtcgtacac ccggtaagaa
     4981 cgccaatgat gaaaagagca tagtgccatg attgatattc aaagccagaa agattctcgc
     5041 aatattgata ttaaaaaggt cggcgtaaaa aatatcaaat acccggtgat tgtacttgac
     5101 cgggccaagg gaacgcagtt ggtcaacgca acgatcaaca tgtatgtgaa cctgccgcac
     5161 cacttcaagg gaacgcacat gagccgcttt gtggaagcgc tcaatgaatt caaaggccag
     5221 atcaatatta aaacgttccg gactattctg gagaaaatgc ggcagaagct gcatgcccat
     5281 tccgcccaca tggaaattga atttgcttat ttcctggaaa aaacggcgcc cgtttccaag
     5341 gcaaagagcc tgatggaata ccgttgtatc tttgccggag aaagcaacgc cgaaacatcg
     5401 gattttctcg tgggagtgat tgtcccggtc acaaccgtct gcccctgctc ccgcgaaatc
     5461 agccaatacg gagcccacaa tcaaagaagt gtcgtgacga caaaagtccg cttcaaaaaa
     5521 tttttctgga ttgaagattt aatcagaata gtggaagatt ccgcgagcgg cgaagtgtat
     5581 tctcttctga aacgcgttga cgaaaaatac gttacggaaa aggcctttga aaatccgaaa
     5641 ttcgtggaag atgttgtccg cagtgtcgcc gtgaaattga acaaggatga caacttcacc
     5701 tggtattgta ttgaagcgga aaattttgaa agcattcata atcacagcgc gtacgctctc
     5761 gtagaaaagg ggtaatggca tgccggcgaa caaatcgaaa aattttttca acctgtatca
     5821 gcatggcttt attcgcgcgg cggtctgcat accggaagtc aaagtagccg atcccgtttt
     5881 caacgcgaag aaaaccatcg aactggcccg ccaggcggcg caacagaaag ccattctggc
     5941 tgtctttccc gaaatgggtt tgtcggccta ttccaacgaa gatctgtttc accaggatgc
     6001 gcttctgcag ggaacgctgc aagccattgc agatgtcaaa aaggcctcgg caagaattaa
     6061 catcgttatg atcgccggcg cgccgctgca ggtcgattgc cggttgttca actgcgccgt
     6121 cgttttctac cgcggacgga ttctgggcgt tgcggtgaaa tcctatctgc caaattatcg
     6181 cgaattttat gaagcccgcc agttcacacc ggcggacgcc gctttctctt caaaaattaa
     6241 actggccggg cagaacgata tcacttttgg cgccgatatc atttttaaaa tcgccaacat
     6301 taaacatttc agctttttta tcgaaatctg cgaagacttg tgggttccgg ttccaccctc
     6361 gagttttgcg gctatggccg gagcgaccgt tatcggaaat ctatcggcct ccaacatcac
     6421 gatcggcaaa tccgaatacc gtcaacaact ggcggcgaat cagtcggcac gttgcgtttc
     6481 cgcttatctt tacgccgcgg caggtcccgg cgaatcgaca acggacctgg cctgggacgg
     6541 ccacgccatg attcacgaaa acggcaacct gctcgccgag tcaccgcgtt tttcacaaca
     6601 agcgcagatc atctacagcg acatcgatct tgaccgtctc gctcaggaca gaatgcgcct
     6661 gacgtccttc agtaaaaacg ccgctgcgca taaagagaaa attttatcct tccgtaagac
     6721 cgaattcgat gtcgatgtgc cgacaggtaa aattctgctg acccgcgaat atccccgctt
     6781 tccgtatatt ccggccgatc cttcaaagcg ggatcagcga tgctacgaag cctataatat
     6841 acaggtacag gggttggcca aacggctgaa agcgtccggc attcccaata tcgtcatcgg
     6901 tgtttccggc ggtctggatt ccacgcacgc cctcattgtc gccgcaagga ccatggatac
     6961 cctgaaattg ccccggaaaa acgtcaaggc gtataccttg ccgggattcg ccacatcgcg
     7021 aaaaacctac aacaatgccc tgaaactcat gacggccctc ggcgtcgagt ccaatgagat
     7081 cgatatcaaa cctgcctgcc gtcaaatgct caaggacatc aaacatccgt atgccaacgg
     7141 caagcaaaaa ttcgatatca cctttgaaaa tgttcaggcc ggacagcgtt cagcgcatct
     7201 tttccgtctg gccaacatgc gcaacgcact ggtggtgggc acgggcgatt taagcgaact
     7261 ggcgctgggc tggagcacgt atgggatcgg cgatcacatg tctcattaca acgtaaacgc
     7321 cagcgttccg aaaacactga ttcagcatct gatccgctgg gtggcccaga ccgggcaatt
     7381 ttcacaggat gtttccgata ttttaatcga tgtgctgaaa acggaaatat cccccgaact
     7441 gattccgggg aaaaacaaag atcatctcgt ccagaaaacg gaagacacca tcggcccgta
     7501 tgagctccag gactttaata atttctatat ttcccgattc ggttatctgc cttccaaggt
     7561 ggcttttctg gcgtatcacg cctggaagga cagagacaaa ggtctctggc ccgataatat
     7621 cgcccgggaa aagaaaaacg cctattcgtt gaaagaaatc aaaaaatggc ttcacgttta
     7681 tttgtatcga ttctttaaaa catcgcagta caaacgctcc tgcatcccca acggccccaa
     7741 ggtcggatcc ggcggctcgc tttcgccgcg aggcgactac cgggcgccaa gcgacagcga
     7801 ggccgaaatc tggctgcaaa actgggcaac cataccggag gaggacattc gatcatgaga
     7861 gcaacctata aagattttga cgccaccgaa ttgttttgtc cccgctgcaa gaaagcggta
     7921 gccgtcagaa aaagattgct gctgattctt caggaaggcg aaaagtacga ttatacctgc
     7981 gtgcattgcg gaacatccat cggcgacaaa ctggtcaaag attctgcccc ttccggtctg
     8041 gtcatcaact gaaattattt atccgatttt tttttcaggg cggttttgag tttttccgcc
     8101 agcaatccgg atccaacagt tccctgagca tcattataga ccaggctcac ttccttgccg
     8161 tgtgcctctc cacccagata ctccgcgaga acctcccggc tgtgaacatc ttcgtggcaa
     8221 tagacacaaa gattttccca gttgcttcca tcggcgggat tgttgtgatg attaccgtct
     8281 ttatgatgga cggttaaaag atgtcttttt gattcgggaa actcccggcc gcaccgcgcg
     8341 caaacaaggc cgttcatcgc cagggacttt tcccggtaat cttctcccga tgaagattgt
     8401 tgagccttct tcatttcccg gaccagcgct tccatcggtt tagccgattc ggacgaaggc
     8461 gcagccggct ttttctgaac ctttacactg ccccacttac tggaaaacgt taccttgccc
     8521 atcgttaacc ttgcttcaat gtctcatctt tctctttatt caaaaggact tccacaaaaa
     8581 cacgcagacc tttggcaatg gacacaacat ttctcataat acggaaaatc ggcaatttca
     8641 gcgccagcgg cgcaaaatga tggatattac tgataatgtc ccccagaagc agacctgaca
     8701 tattcgaggg atgggcaaag ttttgaatct ttttgttgat gaccgaggag gagctgttga
     8761 cgttcgtggt aatttcttcc agatttttca ataacagcgg cagactttgg ttgaacgttt
     8821 gcagtgtcat ctccagatct ttcgatatgc gccagatttg tacaagaagc ggaatacaac
     8881 cgacagccaa aacgataaga atgccaacca aaattaacaa ggcgatttcc agagtcattg
     8941 attttctcct ttaaagatcg ttttttcgaa tcctgttatg atgtttataa accagacagg
     9001 gtcagatttt ttctttaccg agttcttctt tatacgcttt caggcccgca tcgatcgctt
     9061 ttttgagctt gtttttattg ctcagcatgg cctcagaccc ctgctgagcc agttcgctga
     9121 ttttcccttc cgcctccccc gccttcgctt tcacggataa gttgacaccc ttcaaacggt
     9181 tcagagaatc ctcgtaagca agcctgctct tttccagcgc cttctcatat tcctctttca
     9241 cacgagccag catttcgtcg gttttgagta aaatatcatc acgtgtttct ttgccgcttt
     9301 tgggagcaaa caaaataccc agagcgattc caatcaggcc cccgataaac agacctttta
     9361 atacgtcacc gtttttttcc gtcataaagc accttcctta taaaaaagta aataccccaa
     9421 agctcgattg acaggataat gattataaaa tttgctttta ctgagtataa catattttta
     9481 aaaattaaac ttcttcagaa aaaaaatcgg acattccttt cggggaatgt ccgatcaatc
     9541 tatcctgctt ttggatgcga tgcaattatt ttgcaggtgc aggtgcaggt gctgcttcgg
     9601 gagcaggtgc tggcgctgct tcaggagcgg gcgcgggcgc tgcttcaggt gccggagcaa
     9661 ccggtgcttc tactgcagga gcttcgactg caggttcttc agcttttttg cagcctacga
     9721 ctgcaagaga caaagaagca acaaaaagca tagcaacaaa aatcgcaaat atttttttca
     9781 ttaacggcta acctcctttc aaagatttca taataaacgg cttttttaaa agcctgttat
     9841 ctgtattgaa cgggaagaat acgcttttta tttgccttgt caagaataat tttcaccgtt
     9901 ttttgcgcct tttcttttcg atattttatg caaagaaatg cccctttcct gcaactcaga
     9961 aaaagagcag ccacgcatgc atcttcttga tattattgtt attcatatta actttcgtct
    10021 atgcaattga aacgagccgc cgtccgcagc aaaacaatgg ctccttctta aaggagacaa
    10081 tcattccttc ctttaattct gtaaaattca gaaaaccatt tttgtgtata ttaccggctg
    10141 atcgaatatg atatataaaa cgggcttgga aacgttgacg tcataaagac aagccgtgca
    10201 ttcatgaaaa agcaagagga acgaggaaca tggaaagcct taaccgatta attgatctca
    10261 tcagaaagct ccgcgcaccc gacggctgcc cctgggaccg tcaacagaaa aaagaagaca
    10321 tcggcaaata catgctggaa gaagcatatg aagccattga tgctcttgaa aagaaaaatc
    10381 cggaggccct gaaggaagaa ctgggcgatg tgctttttca gatattattt atttgcgaaa
    10441 tatgcgctga gtcaggcttg ttctcccttg aggatgtcat ggacggcgtc aacgaaaaaa
    10501 tgatccggcg gcatccccat gtattcggca ctgtcaaagt aacctccgtg caggatgtca
    10561 aagacaactg gcagcagatc aaaaaaaagg agcgcaagga aaccggccgg gagaaaagtc
    10621 tgttctccag cataccgcgc tcgcttccgg ctctgaaaag ggcgcagaaa attacggccc
    10681 tggcatcgca atccggcttc gactggaaag acatccggag tgtccgggat aagctggaag
    10741 aagaatggca ggagtttgag gctgccgtaa gggcagaaaa ccgcatcaaa acggaggaag
    10801 aactgggcga catattattc acgatggtca atttaagccg gtttctgcgt gttgacgcgg
    10861 aaaccgccct atccaaaacg acagataaat ttctgcggcg tttcgctcat gtcacagaac
    10921 agctatcatc ccgcggctta aagccggaag aagcaaccct gcaacagatg gacgctttat
    10981 gggatgaagc gaaaaagaag gattgaaaat gaaggatttg ctttgcgtgc tgggactcgt
    11041 ctttattatt gaaggacttc cgtattttgt tttcccggaa aagttaaaag tatacctcgc
    11101 caaaataatg accttgccgg attcgacgtt gcggttcatc ggaatatcgt ccatgattct
    11161 cggtttgatt cttttgtact tcggcagacg ctgaaaacaa tggcattgac attaaaatca
    11221 caatctggtt acctgccagc actttttcat gatgacagcc ctatgcaatt aaaagatttt
    11281 tcatatgatc tgccggagga attgatcgcg cagcatccct gtgcaaggcg cgaccaatcc
    11341 cgcctgcttt tgatgggaag gcacaaggat accctggcgg atatgtcctt ttttcagttg
    11401 ccggacttgc tgaaaaaagg cgacgtcctc gtcgtgaacg attcccgggt catcccggcc
    11461 aggctgttcg gcagaaagca aaccggggga cagctggaaa ttctgctttt gaacgtgaaa
    11521 aaaaaggagc cgacctccca gatatgggaa gtgctgatca gaccggccaa aaggctgcat
    11581 gaaggggatg atatccgtct ggcgggtcat tgcgaggcaa aagttttagc caggatttcc
    11641 gaaaaaaaat ggctgctgga attttctgct ccggacggtt ttgaatccta tgtgaacacc
    11701 ttcggcaggg ctccgcttcc cccctatata aaaaggatga aaaatacccg gcccgatgct
    11761 gccgatctgg atcgttatca gaccatttac gcgcggcatc ccggttccat cgcggctccg
    11821 acagccggtc tgcatttttc agacggggtc atacagcaat taaaagaccg gggcatccaa
    11881 attgctcacg tcaccttgca cgtgggttac ggcacgtttc tccccatgac aacggacgag
    11941 gttgaaaaac atgtcatgga accggaacat tacgaaatca gtcccgaaag cgccagaatc
    12001 atcaacaacg ccgagcgcgt tattgcggtg ggcaccacat ccacccgcac gctggaaagc
    12061 gtttcaaacg aagacggata tataagcgcc cgatccgggg tcaccagttt atttatttat
    12121 cccggttaca aatttaaaag aatcaacggc ctgctgacca attttcattt gccgcaatca
    12181 tcgctgtttc ttctggtctg tgcttttgcc ggcaccgatt tgatcaaaaa agcgtatgcg
    12241 catgccgtaa aaaaccgtta tatgttttac agctacggcg attgcatgct gatactttaa
    12301 cgccgccatt ttttacggga agcaaatgcg tgcacgcgtt gatcccgttt accggagcca
    12361 ggacataaaa atgtttcgct ttgacgtaat caaaaaagac gacaagacgg ccgcccgcat
    12421 gggagagatc aaaaccgccc gcgggatagt gcagacgccg gtttttatgc cggtcggcac
    12481 gcagggaacg gttaaatcca tgctgccgga agagataaaa aactgcggcg cgcaaatgct
    12541 tctgagcaac acgtatcatt tatatttacg tcccggtcat gaaaccatca aagaactcgg
    12601 agggctgcat cgttttatga actggcctta tccgatcctg accgacagcg gcggttttca
    12661 ggtttacagt ctcggaaccc tgcgaaaaat cacccccgag ggcgtcatgt ttcgttcgca
    12721 tattgacgga tccagacatt ttctcacacc gcagaaagcg atcgaaatcc aggaagcgct
    12781 gggatcggat atcatgatgt gcctcgacga gtgcacgccg tatccgtcga ccccgtccca
    12841 aaccgaaaaa tcgctgaatc tgaccgcaaa atgggccctg tcatgccggg aggcgaagac
    12901 aaacaacggg caggcgcttt tcgggatcat tcagggaggc tcgtatcctg atctgcgcag
    12961 gcaatccctg gaaaaaaccg ttttgctgaa cttcgacggc tacgccctgg gaggcgtcag
    13021 cgtcggcgag cccaaggaaa tcatgtatac cataaccgat gtaatggctc ccctgctgcc
    13081 ggagaataaa ccccgttatc tgatgggcgt cggcacgccg gaggacattg ttttcggtgt
    13141 atcttgcgga atcgacatgt tcgattgcgt tattcccacc cggtgcgccc gtcacggatt
    13201 attgtttaca aattcggaaa atgttgttat aaaaaacgcc cgctggcggg aagacgctga
    13261 tccgattgat gaaacgtgtg attgctacac ctgccggaat tattcccggg catatttaag
    13321 acatctttat ttcgccggtg aaatactggc aatggttttg aacaccattc ataatgtccg
    13381 ttattatatg agactgatgg aaaatataag gctggccata caagaaaacc gttttgaagc
    13441 atttaaaaac gcttttctga aggacaggac cgaatcgaaa aaacatctaa catagagcaa
    13501 atcgcaagga ggatatgaca ttgacatcgt tagcatatgc aatgggcgga gcgcccggcg
    13561 gcccggcggc cggaggaggc ggagactggg gattcatcat caccatggtg atcatttttg
    13621 taatttttta ctttctgctg attcgcccgc agcaaaaaaa acaaaaggaa ctcaaggcca
    13681 tgctggataa tctggcctac ggcgatatgg tcatgacgac cggaggcatc cacggcaaaa
    13741 tcaccggatt gacggatgca atcgttaccc tggaaatcgc cgacaaggtc agaatcaaag
    13801 tcgcccgcag cgccatcggg gccattgttc agaaatccgg ttcggcagcg ccggcagcgc
    13861 caaccaagtc ataattttta agaaaggggc attttcatgt tcaaatcctt aaagctcaga
    13921 ggagctataa ctttaatcgt gtgtctcggc tgtctttatt ttctgattcc gacatttttt
    13981 tccaatatcc cctccccctg ggataagtac atgtttaaag aaaaaattca tctggggctg
    14041 gacttgcagg gcggcatgca tcttgttctt gaaatcgaca cggataaagc actggacgcc
    14101 atggtcgagc gactctccaa cgatttaaaa gaatctctga tggacagcaa ggtgcgcttc
    14161 cgcaatctgg aaaaaatgca accggcaaaa atttccatgg agctggccgg tgccgatgaa
    14221 gaaaaggggc tggaaaacgt cctgcgcgaa cattaccccg atcttgaaat agaatccgcc
    14281 agtccgcggg aagacggaaa gtttgtcacg ttacgaatca acgacaaacg cgccgccgaa
    14341 ttgaaaaaat taaccgtgga acattctctg gaaacgatcc ggaacagagt ggatcagttc
    14401 ggcatctcgg agccggaaat catcccggaa ggagacgacc ggatccttgt tcagctcccg
    14461 ggcattaaag accccgaaag ggcaaaaaat ctgatcggga aaaccgccct gctggaattc
    14521 aaactggttg atgacgaaaa ctcgctggat gaagccctgc gcggcaacat ccccgaaggc
    14581 gatgtgatcg cctacggtct gcgcgccgat caatcgaccg gagtcagagg ctcttctccc
    14641 tatctgctca aagacaagtc cctgatgacc ggcgcttccc tcgaaacggc caaggttcag
    14701 attgccgacc gtttcggtga tgtgaccgtc ggcctgaaat tcaattctca gggcgctgtc
    14761 gattttgaaa gaatcaccgg ggaaaatatc cgcaaaaggc tggccattgt tctggacggc
    14821 gttgttcatt ccgcgcccgt cattcaggag agaatatccg gcggtcaggc gcagattacc
    14881 gggaacttta ccatggaaga agcgcgcgat ctcgccattg ttttgcgggc cggtgctctt
    14941 cccgcgcccg tcaatattct ggaggaaaga acggtcggtc cttcgctcgg cagcgattcc
    15001 atccggcaag gcgtcatggc caccatgatc ggatcgcttt tggtcatttt atttctgatc
    15061 atctattatc gcctttccgg cgcaatcgcc gacgttgccc tgatcacaaa catttttctt
    15121 gtactggcca ttcttgcggc cttcagggcc acactgacat tgccgggcat tgccggactg
    15181 cttttgacgg tgggtgtcgc ggtggacgcc aatatcctga tctttgaacg gattcgtgaa
    15241 gaactgcgca cgggcaagac catcagaatc gcactggata cagggtataa tcgggccttc
    15301 attacgatta ttgacaccca catcacggga atagtcgccg ctgcttttct gatcatgttc
    15361 ggaacaggcc cgatcaaggg atttgccgta acaaccatca tcggcctgct ggccagtctt
    15421 tttacggcag ttttcgtcac cagggtaatt ttcgattata tcaccacgaa ctttaacatt
    15481 aaaaagctga gtatttaaaa atatacaggc aggcaggaga catgatatgc agttgataaa
    15541 accgggaatc aatctggatt tcgttggaaa aataaaatac gcgataacat tttcagtcat
    15601 tcttatcatc gtcagtatcg gatcggttat ttatcatggc gggttaaatc ccggtattga
    15661 ctttgccggc ggctcggtca ttcaggtaaa attttcaaaa aatatttccg ccgatcaaat
    15721 ccggggggct ctcaaatcga ccaggcttca aaacagcacc attcaacagt tcggaaccaa
    15781 cgaattttta atccgcacgt cagaatcttt ttcggatcaa acggtgctgg ccgctaacgt
    15841 tgagcagccg ctggccgccg cgttcagtaa agattttgaa atcaggcgcg tggaggtcgt
    15901 cgggtcaaag gtcggcgccg acttcaaacg taaagccgtc attgcggtcg tcttgtcctg
    15961 gatagccatg ctcatttacg taggctttcg tttcgaattc cgctacggcg tgggaggcat
    16021 cgttgcgctg gttcatgacg tcatcattac gatcggagct gtttccatca tgaacatgga
    16081 atttaccatc tccatcctgg cggcgctggt ttttctcatc ggtttttcga tcaacgacac
    16141 catcgtcact ctggacagaa tccgggaaaa tgttaaattg aatcccaaaa gacaactccg
    16201 cgagaccatc aatctgagca ttaatcagac gctcagcaga accgttctga cctcactgac
    16261 cgtttttctc acggttctcg ccctgtatct tttcggcggc gcggtaattc gcgatttcgc
    16321 cttcgcgctt ctggtaggtg tggtcatcgg aacctactcc tccatttaca tagcctgtac
    16381 gattgttctg atgtttgaaa accttcaaat ttcgaaaatg aagaggactc cataatttgt
    16441 cattttgggc ggcagcggga cttacaattt ttattctgat actgtttgcg ggtattttct
    16501 tgaatttcgt cgatttgccc ggaaccgttg tcattttttt tgatgttgtg ttgtatgcga
    16561 tcttgaccgg atttcagcaa gtcggctggc aaattattgt actgcttcta atttgcgccg
    16621 tggttgccga atccctcgaa tttttctgga ctatccgtga aacgcctccg caggagcacc
    16681 gtcctctgaa atccctgaaa gcgacgatca tcggagcggt gacgggagcc ttcctgttga
    16741 cccccttttg ggcagcgccg ggtgcttttg ccggtttcct tctgggaggc cttgccggca
    16801 ttctgatgat ggaaatcatc aggcactgcg aagcaaaaat tcatcattgg acgctcagca
    16861 gtgttatttt taataccatc gggaaaaacg ccgtcaaagg atttatatcc ctgttcatga
    16921 ttgccttttc tctatccaac atttattcct aggagtctgt aaccatcatg acggaagaaa
    16981 acaaaaccaa atcaaaagtt aaagaatatg tcgaatccat catcattgcc attctgatcg
    17041 ctttgtttat cagaacattt atcatatgcg cctataagat accgtcccgt tccatggtgc
    17101 cgacactgct ggtcggcgac catattctgg tcaataaatt catctacggc gtgaaaatcc
    17161 cgctgttacg aagaaccatt attcccgtca gcgaacccaa aagaggcgaa gttgtcgttt
    17221 ttatttatcc caacgaccgg tccaaagatt tcatcaaacg ggtgatcggt gttgccggcg
    17281 acaaaattga aataaaaaac aagaaaatat tcatcaatga cgaggagttc aaggattcct
    17341 acggcatttt cagcgatagc ctcgttttac cgcaatccct gcagccgcgg gacaatttcg
    17401 gaccgatcat cgttcccgag aaatccattt tcgtcatggg cgacaaccgc gatgaaagcc
    17461 tggacagccg tttctgggga tttgtcaacc tgaaagacgt cgaagggaaa gcctttgtca
    17521 tttactggtc ctggaaccgc gatgagaaca atcttcgctg gaaccggctg ggtcaattgc
    17581 tgcactaggc cgttctccgg aaggtttcgg cgctttccgc cgtttgtcat tgcgtcatat
    17641 cagcagacat tcgggaaatt attttaaaat cttgacacac aaacggtttg gttatataac
    17701 catccccaat aaagcctttt aatttaatcc ggagagattg aaaaaggaaa cgttgatgag
    17761 aaaatcaaag atccatgaag attatgaagc ctttaccgtt ttggggaaag gctttttttt
    17821 taaccgggca aggggttagc gcacatgtcg aatacgaaaa gcacaaaaac cgtcatggat
    17881 aaggaaggca ttgagcggtg cctgaccaga atcgcctatg aaattctgga aaagaacaag
    17941 ggaatggaaa accttgtcct ggtcggcata agaacgggcg gcgtgtatct cgccgaacgg
    18001 ctgcaaaaga aaatgtccga aatcgagcac ggcaagatcc cgatgggcat actggatgtc
    18061 accctttatc gcgacgatat ttccgtgtcc aacaaaaaac cgcggctggg cgaaacgaaa
    18121 ataaattttt cactggatga aaaaatcgtc atccttgtgg acgacgtgct cttcaccggc
    18181 agaaccatcc gcgcggccat ggacgctctg attgatttcg gacgcccccg gatgattcaa
    18241 ctggcggttc tcattgaccg cggtcatcgc gagctgccca tccgggcgga tttcgtgggc
    18301 aaaaatctgc cctcctcgct ctgggaagcc gtccgggtcg acctggtgga aaaaaacggc
    18361 gtcgatgaag tcgtcattga agaaggaaca taaacaagaa ttttttaagg ccccctatga
    18421 aatttgaacg aaaagatatt ttaggaataa aagatttatc ggtcgatgaa atcaacctga
    18481 ttctggaaac ggccggatct tttctggagg tgtcgacgcg caaaataaag aaagtgccga
    18541 ccctgcgcgg gaaaacggtc atcaatctgt tttacgaagc cagcacccgc acccgaacat
    18601 cctttgaaat agccggcaaa cgattaagcg ccgacacgat caatatttca tcgtccacca
    18661 gctccgtcat gaagggagaa acgctgatcg acacagcccg caacctggaa gccatgaatc
    18721 ctgacgtgat tgttatccgt cacagcgccg ccggcgctcc gcatcttctg gcgtcgctgg
    18781 tgaaacagtc gatcatcaat gccggcgacg gcgcacatga acatccttca caggccctgc
    18841 tggacatgat gaccatcaaa gaacgcaaag gaaatttccc cggattgaaa gtggccatca
    18901 tcggcgatat tgaacacagc cgggtggtcc ggtccaacat ctacgggctc tccaaaatgg
    18961 gcgccaccgt cgttgtggcc ggtccggcca ccatgctgcc ccgcgatatt gagcaaatgg
    19021 gggtcaaggt ctttgataaa atggaagacg ccatcaccga tgccgatgtg gtcatgatgc
    19081 tgcgcattca actggagcgg cagaagcaaa acatctttcc gtctctgcgt gaatattccc
    19141 agcgctactg cctgaaccgc aacaacatca aactggcgaa aaaagatgtt attgtcatgc
    19201 atccgggacc gatcaaccgc ggtgtcgagt tatcccccga cattgccgac gatccctctt
    19261 tctctgtaat tctcgatcag gtaaacaaag gcgttgccgt acggatggcg ctgctctacc
    19321 tgctcaccgg aggtagcgaa tgaaactctt attgaccggc gcccgggtca tcgatccggc
    19381 gcaaaagata gattcccgcc tggacgtttt tctggaaaac gggaaaatcg cccgggtcgg
    19441 cgccaatctt tccaaatcca caaaatccaa agacgcggaa aagataacaa agattatcga
    19501 tctggccgga atgatcattg tacccggcct gattgacatg cacacgcatt tacgcgaacc
    19561 tggcttcgaa tacaaggaaa ccgtggcttc gggtgctgcc gccgcggtgg ccggaggctt
    19621 tacgtctatc gcctgcatgc ccaataccaa ccccgtcaat gataaccgct ccattacgga
    19681 attcatcaag gcaaaggcgg cagaagccaa cctcgccaac gtctatccca tcggcgccat
    19741 cagcagaaat tcagacgggg atcagctgac ggaattctgg gacctcaagg aagcgggcgt
    19801 gattgcgctt tccgacgacg gcaaaccggt catgaatgcc gctctgatgc gccgggccat
    19861 ggaatacgcg cattcgctgc aattacccgt tattaatcac tgtgaagaca tccatctttc
    19921 cgcaaacgga gccatgaatg aaagttatta ttcgacgatt ttaggattaa acggtatccc
    19981 cgccatcgcc gaagaagtga tggtggcgcg ggatattttg attgccgaat ataccggaac
    20041 ctccgtgcat atcgcgcatg tcagtaccgc aggttccgtc cgtttgattc gcagtgccaa
    20101 ggaaagaggt gtaaacgtca cggcggaaac agccccgcac tttttcacgt taaccgatga
    20161 ggccctgcag ggctacgata cgttttttaa agtgaatccc ccgttaaggg gcgaaaagga
    20221 tgtcaaagcc atcaaggaag gactggccga cggaacgatt gatgtcattg cctgcgatca
    20281 cgctccgcac gcgcgcaccg ataaagaagt ggaattcgaa tatgccgcca acggcatcag
    20341 cggattggaa acatcgctgt cgctgagtct gaacctgatc cacagcaaaa tcctgagctg
    20401 gccggaactg gtttccagga tgagcacccg gccggcgcgt atcctgaacc tgccgaaagg
    20461 aacgctgaaa gaaggcgccg atgcagacat cacggtcatt gatcccctgc tgccgtggac
    20521 agtggatgtg caggcgttcc ggtcgcgggg taagaattct cctttccacg gacgtcagat
    20581 gcagggcaaa gccctcatga ccatcgtcgg cggtgaaatc aagtatgacg gccgttaggg
    20641 aacattttaa aactaccggg tttatcctgc gcaccctgag ttacagcgaa tcggatttaa
    20701 ttgtcagctt ctacagtcat gacttcggca agctcaaggg aatcgccaaa ggggccaaga
    20761 gaagcaaaaa aagatttgcc aacgtctttg aaccgttttc cctgaccgat atcattttta
    20821 cccgaaaaag ccgcgacacc ctcgcgttca tcgaatcgtg cgacatcgtc gaccactacg
    20881 acgccatccg cgccaatatg gagaaaacgc ttatcgcgtc ttacttcatc gatctcaccg
    20941 accacttcag cccggaaggg aaaaagaatg aagatatatt ccgattgctt caggattttc
    21001 tcgtcctgct gggcagggaa aaggtctccg attcgattgt ccgttttttt gaaatccgtc
    21061 tgctgaaact ggccggtttc gaaccggcgc tcgatcactg cgtgatttgc aggacgccgg
    21121 tgaccaacgg caatgcctat tttttcaacg ccggccgcgg cggcatcacc tgcagcgcct
    21181 gcgcgaaaac gcaaaaatat gagcagccga tttccgcagg gaccttgcgc accctgcttt
    21241 tgggaaaaga catggacatc gataaaatca aactggtcac cctgaccgat tcgtcagcta
    21301 cggaatcacg ctgcctgctc acgggtttca tatcccatgt gctcggacgg gaagtcaaat
    21361 cgtggaaagt cctggaacag gttcgtcgat actgcacatg atggaaaatt aaaatgcatt
    21421 ctccggaatt attgattccc gcggggaatc tggaaaaatt gcgcacagcc ctgct
//
LOCUS       DAONQZ010000040        21037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2844_length_21037_cov_16.597417, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000040 DAONQZ010000000
VERSION     DAONQZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21037
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2844_length_21037_cov_16.597417"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..951)
                     /gene="ispE"
                     /locus_tag="PLR13_07665"
     CDS             complement(112..951)
                     /gene="ispE"
                     /locus_tag="PLR13_07665"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQC19252.1"
                     /translation="MHWIAPAKINLFLKILRRRADGYHDIFSLMQKISIYDELIFAPL
                     HRGIVLHCPGMNLPENDGNLIVRAAKAIFAYANYSGGIEITLIKKIPMTAGLGGGSSD
                     AATTLLALNKICALKLKKNELMAIGAKIGADVPFFVFGNTALATGIGDKLKHLRNLPP
                     LNLVLIKPPFDISTKMVYESLNLRLTMGKNNYSIPRFLKLGDIVQVLQNDLESVSLKI
                     YPELDEFKKMLLGHGALGALMSGSGPTIFGIFRNGKEAKKALECINKEIPGNYTVFFA
                     KSL"
     gene            complement(953..1351)
                     /locus_tag="PLR13_07670"
     CDS             complement(953..1351)
                     /locus_tag="PLR13_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HQC19253.1"
                     /translation="MEDKKQGSGFVIKDKRHFGESAEEQKQEETKTAGAEEKQKGPEP
                     TKQGESDYPPVNFTNFILSLSTSAFFHFGDLAESEDAAPQKNLPAAKQTIDILDMLNE
                     KTKGNLDKNESGLLQGIIYELKMRYVKEKG"
     gene            complement(1385..3976)
                     /gene="clpB"
                     /locus_tag="PLR13_07675"
     CDS             complement(1385..3976)
                     /gene="clpB"
                     /locus_tag="PLR13_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQC19254.1"
                     /translation="MRFDKFTLKVQEALQEAQTLAGSMGHQSIEPEHLLVCFLRQEDG
                     IAGAIINKLNASSQKIEKELDGYLKKQPSVGGAGAGQVYLGGRLNKIFDKAMTEAAQL
                     KDEYVSAEHVLVCIAEEKEGQAGKILRQAGVTKDNIFKVLKDIRGNQRVTDPNPEGKY
                     QALERYARDFNALARKGSFDPVIGRDDEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKNKRVIGLDIGALVAGAKYRGEFEERLKAVLKEVISAEGEII
                     LFIDEIHTMVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYITDRFLPDKA
                     VDLIDESASRLRIELDSMPSEIDAIDRRIIQLKIELQSLKKESDKTAKERRDKIEKEI
                     ADLQEKMDQMKVHWSGEKDIIKKIQNIKSRIESLKTEEANAQRDGNLEKAAEIRYGKL
                     VALDKELQNEQKKLEDIQKNNQMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLLH
                     MEERLKLRVIGQEEGINAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSQWIQDPSLDDEEK
                     RNRSMDALRATFKPEFLNRIDDIIMFSALKLEDIYKIVDIQMGLIDKRLKERKMSVEL
                     TEKAKNYIAGQGYSPVYGARPLKRSLQKLILDPLAMEILAGKFAEGDKILIDQSKKGE
                     ITLTKKS"
     gene            complement(4328..5200)
                     /locus_tag="PLR13_07680"
     CDS             complement(4328..5200)
                     /locus_tag="PLR13_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HQC19255.1"
                     /translation="MKTINKTMIDHAVKIAREIKANALLVCADVVSDVSLIMDEIEKD
                     IAFIMVSREDESLSEEMKKDAKLLYIPNVNLTRVGIIKIAIAKGIILGALKRGDKVVC
                     LSGVPKFGYADSIFVIDVGKEFEILTSDFINDVLENVRPEVFNAVLNIALELAAQGRE
                     SRKVGTIFILGDDEKVMQLSRQMIINPFLGYPEDQLNILNPDLEETIKELSAIDGAFI
                     IKENGVLVTAGRHLSAALDSKDFPPGLGSRHIAAAGITSLTRAVAIVISESSGNVTIF
                     KNGKLFVTIEKPVE"
     gene            5414..6268
                     /gene="rpoH"
                     /locus_tag="PLR13_07685"
     CDS             5414..6268
                     /gene="rpoH"
                     /locus_tag="PLR13_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HQC19256.1"
                     /translation="MTALEFPVVTGTLNLYIAEINRFPLLTAEEEFKLAVRLKKYNDV
                     SAAEKLIVSNLRFVVKIAHEYRNYGFKLSDLIQEGNIGLIHAVKKFDPYKGYRLISYA
                     VWWIRAYIQNYLIRSWSIVKIGTTQAQRKLFFKLNQTKKELENLSRKNPEFSEIATSL
                     GVKDSEVEEMDLRMGARDVSLHEIINDESSNSHIDFLAYEGDDQEISLIKKEEKSLVQ
                     RNIAGALANLNERESYIILNRVMSDNPKTLQEIGEKYNISRERARQIEKQALKKMQLA
                     LPYFKPNK"
     gene            6386..15112
                     /locus_tag="PLR13_07690"
     CDS             6386..15112
                     /locus_tag="PLR13_07690"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQC19257.1"
                     /translation="MEKIIFFKYFHRLKSRLAGKHQNEKLTEDELLRSELFSAKQMRQ
                     HGKTIAGAHKLNEGLTSNQLLERLTENEKVLFQAREKLTKAVLENRRVTPAEEWFLDN
                     FYLIEEQIRTARRHLPKVYSRELPHLLNGPSANLPRVYDIALEMISHGDGRVDSKTLS
                     LFVSSYQSVTVLKIGELWAIPIMLRLALIENIRRIASRISKDRTNRDLADYWARKMIA
                     TVHKDPKNLFLEIADMVRSEPPIAGPFVAEMARQLQGQGPALALPLTWIEQQLSEYGQ
                     TIKNFVQMESQQQAADQVSMSNSIVSLRFLGTMNWREFVETMSFVDQVLREDPADIYV
                     KMDFATRDRYRHVIENIAKKSSLSETEVARMAILLARKTSNQKGGQDRTAHVGFYLID
                     NGLPELERLTKMRFSLFESVARFSRRFPLLIYVGSIFLLTFTAAFLLLAMAYADGLTG
                     WLLAVIWLVALLSTSHLSIALINWVSTLLITPAPLPRMDFSRGIPPEYRTLVAIPTML
                     LNDKNVGKLIESLEVRFLANRDENLRFALLTDFCDAKTEKMSDDGQLLRLVQKGIESL
                     NEKYADDRGDIFFLFHRSRRWNPKENIWMGYERKRGKLAELNAFLRGITHGPEGGLFS
                     LIVGDTQELRNVKYVITLDTDTKLPRDAAWQFVGTMAHPLNRAQYDEQKRRVVQGYGI
                     LQPRVSVSLPDISRSLYSIMHGSSSGIDPYTRAVSDVYQDVFGEGSFIGKGIYDIDAF
                     EASLNGRFPENYILSHDLIEGCYARSGLISDVQLYEDYPSAYKADVSRRHRWIRGDWQ
                     IARWIMSRVPSADGERLGNPLSVLSQWKIFDNLRRSLVPASLTLMLILGWLLSSSPGL
                     WTLAVLSIIVFPALVMSLMSVFQKQQNVILTHHLSSVFQKTGNNFLQVLFSFICLPYE
                     AYFNTDALLRTLWRMLISHKRLLEWTPSENTNNQRDDLAGYTRTMWFAPSLAAAVFAA
                     LLTAVPTGVPSAAFCLILWLLSPAVAWWFSQPSRLREARLSSAQINFLRKASRKTWAF
                     FEEFAGPEDNWLPPDNYQEHPVEVVAHRTSPTNIGLSLLANLTAYDSGYITVLKFIER
                     SSNTLRTMTSLERYKGHFYNWYDTQSLDPLLPLYVSSVDSGNLVGYLFILKSGLSSLP
                     DKKIIDPQILDGLKDTLGILNDSTGKSVSANLSQISKTLDDATASPPAKLTEMKIVLE
                     KIISLAEETASCMRPESDASNAAGHWLKAFLQQATAALDEMLFLTPWLFDPQFSDLIR
                     EVPELDDIMTLRELTSLYEHVESKTEAMKRFESTAIDLSRISDLKNLIAESGNRAVNL
                     MQNIDLLARQCTEFTDIEYDFLFDKSCNLLSIGYNVTNSRRDTSFYDLLAAEARFSNF
                     VGIAQGKLPQESWFALGRLLTMAPGKKPVLVSWSGSMFEYLMPLLIMPTYEGSLLDQT
                     YKAAVAAHIEYGKKRSVPWGISESGYYAFDSHLNYQYRAFGVPGLGLKRGLAEDLVVA
                     PYASALALMVEPEKACLNLEKLASREFQGKFGFYEAIDFTPSRLTRGQSNAVIRSYMA
                     HHQGMTLLALTYLLLDRPMQKRFEAEPLFQSALLLLQEMVPKAVAFYASPSELISAQK
                     EPEISENPVRVFNTPDTTVPEIQLLSNGRYHIMLTNAGGGYSRWKNLDVTRFREDVTC
                     DSSGIFCYLSDVKTGDIWSTTYQPTLKKPRNYEAIFSDGRVEFRRRDYEFDVHTEIVV
                     SPEDDIELRRVTITNQSRLQKTIDITSYAEVVMTHSAADKLHPAFSNLFVQTEIIEQR
                     RAIVCTRRPRSEQEINPWMLHSMAVHGQDIEQISYETDRSRFIGRGNTLADPQAMGPP
                     SDLFGTLSDSQGSVLDPIVAIRSRITLGPEQSAVVDMVFGIGQTRDEAITLVDKYQDR
                     RIADRVFEMAWTQSQVLLRQINATEADAQLYSHIASSLIFPNDSLRAGAGVISENRRG
                     QSGLWGYAISGDLPIVLLRVSDQSHIELVRQLIQAHAYWRLKGLAVDLVIWNEEHAGY
                     RQLLHDQIMGLVSSGFVADLKDQPGGIFVRSTDQMPEEDRILIQTVAHVILSGNRGKL
                     ANQVKQRGGLKTAAPRMIPNRTHREPPVPKAAPPRSDLVFFNGLGGFDPSFREYVITS
                     SKSQITPAPWINVLANPTFGTVVSESGMSYTWADNAHEFRLTPWHNDPVSDRSGEAFY
                     IRDDERGHFWSPMPLPHRGLEPYTTRHGFGYSVFEHTERCIQSEVWIYTALDASVKFI
                     VLKIKNLTERTRRISITGYIEWVLGGLREKNAMHIITEADHQSGAIFARNSYNMEFPN
                     RICFFNSSASIRTITGNRMEFLGRNGTLADPAVMKRQYLSGKVGAGLDPCAAIQVPLE
                     MLEGEEKEVVFTLGMGRNVTEARKLAQFFSDPATAADALQAVQNYWREKLDAIQVQTP
                     DPSVNVLTNGWLLYQTLSCRLWARTGYYQSGGAFGFRDQLQDVMALIYTEPNFAREQL
                     LLAASRQFEEGDVQHWWHPPLGRGVRTQCSDDYLWLPLTTCCYISRTGDQGILDEIIP
                     FINGRPLNREEDSYYDLPVRSPEKANLYEHCLRAIKRGLRFGEHGLPLMGSGDWNDGM
                     NMVGIQGKGESVWLGFFLYDILTQFAKIASRRGDTSFEECCRKEALQLRLNIEQHGWD
                     GLWYRRAFFDNGSMLGSARSEECRIDSIAQSWSVLSGAGNGHHSRLAMESLSQHLIRR
                     DIGIVQLLHPPFDKSGLNPGYIKGYVPGVRENGGQYTHGAVWAAMAFAALHDNNRAWE
                     ILTMINPVNHANSPESIAIYKAEPYVVAADVYAFSPHTGRGGWTWYTGAAGWMYRLIT
                     ESLLGFKKEDDTLYIEPCLPAEWGGFSMHYRYKKTFYRIVVTVTPERKKEKTVLLDGI
                     RQEEKAVLLTNDGIDHQVDVIL"
     gene            15259..17502
                     /locus_tag="PLR13_07695"
     CDS             15259..17502
                     /locus_tag="PLR13_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQC19258.1"
                     /translation="MRKYILPLLALILLFRIGYTFFDVGRGLSEATAEGPSIFYGRST
                     VIKKGVHLENMRLPDRLRRLSYKKVAGPPYAAGTFSEEQSRWRIFLKNSPDRNPSEDN
                     GPVEIVLRDGRVDAIISSTGKTLTSIQLEPEEIVRIIDEKMPSRPAVGLSAISPFLQN
                     AVIASEDARFYSHMGIDLPSVVKAFFSDPAKRNFPQSSSTITQQLARNFFLSPEKTFA
                     RKLRESELAFVLELKYTKKQVLEMYLNRIYWGQDGSRSIYGAGSAAGFYFSKSAAHLS
                     LEEASLLAGILHSPNRYRNFKAAKERRNAVLSRMKDLKMISDGEFQRACKAPVRVKLS
                     KAPAQTSYFIDFIQRITKDDLGSEKLYHRGYRYYTTMDPVVQAIADEALAEGIREIER
                     RAFPAGEKLQAALVAVDPATGEMIAMVGGRSYRETQFNRAVDANRQSGSAFKPFVLLA
                     ALSLSLEQGKDITLSSTVSGKPLSINTPEGMWRPTNFENKHYDRITIRKMIEDSVNTA
                     TTRLAGEIGFREVLKAARRAGITSPLSTVPSMALGSFEVTPRELAYAYTTIASGGIRF
                     DPFALYSVKMANGRTIISKTVRREKAFDPRIIYLAGYAMQGVLERGTAAEAKALGIYF
                     PAAGKTGTTNGNRDSWFVGYTPDIVCAVWVGYDSGADTGLTGARGALHIWSRFIRSFY
                     GRSAPANIVPPAGIETAIIDPQSGYLATTSCPQKFKEAYLKGTAPKKACPDHPVKPVM
                     KRIYRML"
     gene            complement(17910..18017)
                     /locus_tag="PLR13_07700"
                     /pseudo
     CDS             complement(17910..18017)
                     /locus_tag="PLR13_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(18481..20148)
                     /locus_tag="PLR13_07705"
     CDS             complement(18481..20148)
                     /locus_tag="PLR13_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQC19259.1"
                     /translation="MRRKTNQAFLFFSHLSSEIIIREFNHIRRAVCDMGDAFFLYDTT
                     GKDVPEKIKKLSPYLYSDQCLCDLGYPVIGKKMIPGHAHFPLFKFYLDHPDFDYYWFI
                     EYDVRFTGPWRSFFESFLVHDADFLTSHIRSYAEERSWPWWALKHPRQHIPLDKRLRS
                     FNPLYRISRAALSFLHDAFRNGWCGHHEVALPTLLHQKGFTVREISGRGKYTIPGMEN
                     RFYAMSAPNVKGDLSGTSMKYRPSSWRCGREENKLYHPVKPLKKAVHENILYYGGFLS
                     HALSDVDASGILLRLKIRHRRILDFVKYRLPKKESFRKPRVGIIITSFNQIDYTDRAL
                     ESFYATINKNIDYELWLLDDSSSEDIEGIYHKYKDRGVKFFKNKTNNGLTSLWNKGIE
                     LNKDKDYLILCNNDVVFSNYWADHLIGSLKASPGFAVAVPVTNAPGHVPAQHIANFVD
                     DYVPSDNQDEINLICTKVKNKNFRKTAKGNGFCFAARASLFRQMSTQGGPFNEKFPLY
                     GSEEDFFRRVKPKTMIVPESFVFHYKHVSVESDYFPDQKFRRKTKSL"
     gene            complement(20177..20905)
                     /locus_tag="PLR13_07710"
     CDS             complement(20177..20905)
                     /locus_tag="PLR13_07710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQC19260.1"
                     /translation="MPEASIIIRTKNEEKWIGECLNRLFNQTYRDFDVVVVDSGSTDR
                     TLEIIGKYDVHLFQIPPEQFSYPNALNFGCSRSSAAKYFVFLSGHSLTVSQNWLKDGI
                     ENFKTADNIAGIYGDVWALPDGSFWEKIIFNRCQIILKNYFRRKRVLKKKQMGILAFT
                     NAIIRRDLWERYPFNENYGLGGEDMEWAGYWLQKGFVVIRDRNVCVYHSHGLGLKQLF
                     QQRKNWISLAEPQPFRVTEFRNGR"
BASE COUNT         5299 a         5516 c         4915 g         5307 t
ORIGIN      
        1 acctccgaat gcaggaacca aaatcctgtg tcttaccgct tgacgacgcc ccaataaaca
       61 tatttctcaa tgaggggtat cgatcacaat ctgattagga atatggaatg attacaaaga
      121 cttggcaaaa aataccgtat aattaccggg aatttcctta tttatgcatt ccagggcttt
      181 ttttgcctct tttccgttcc taaatatccc aaagattgtg gggccgctac ccgacatcaa
      241 tgcgccaagc gcaccatgtc cgagcaacat ttttttaaat tcatccagtt ccggataaat
      301 tttcaacgaa actgactcca ggtcattttg taatacctga actatgtcgc ccagtttcaa
      361 aaatcgtgga atgctataat tattttttcc cattgtcaat cttaaattga ggctttcgta
      421 aaccattttt gtggaaatat cgaaaggagg cttaatcaaa acaagattca gaggaggcag
      481 attgcgtaag tgcttgagtt tatcgccgat tccggtagcc agagcggtat ttccaaaaac
      541 aaaaaaaggg acatcggctc ctattttggc cccgattgcc atcagctcat ttttttttag
      601 ttttaatgca catattttgt ttaaagccag cagtgttgtt gcggcatcgg agcttcctcc
      661 gcccagaccg gcggtcatgg gtattttctt gatgagagta atttcgattc cgccggaata
      721 attggcatag gcaaaaatag cctttgcagc gcgcacaatg agattcccgt cgttttcggg
      781 cagattcatc cccggacaat gcagaacaat gccccgatga agaggagcga aaatcagttc
      841 atcataaata ctgattttct gcatcaatga aaaaatgtca tggtatccgt cagcgcgtcg
      901 tcgcagaatc ttcagaaaaa gatttatctt cgccggcgca atccagtgca tatcaaccct
      961 tttctttgac gtaacgcatt ttcagttcat agatgatccc ctgaagcagg ccgctttcat
     1021 ttttgtcgag atttcccttt gtcttttcat tgagcatatc gagaatatca atcgtctgtt
     1081 tggccgccgg caaattcttt tgcggcgcgg cgtcttccga ttccgccaga tcgccaaaat
     1141 gaaaaaacgc ggacgtactt aatgaaagaa tgaaattggt aaagttcacc ggcggataat
     1201 cagattcacc ctgtttagtc ggctccgggc ctttttgttt ttcttcagct cccgccgttt
     1261 tggtttcttc ctgtttttgc tcttcggctg attctccgaa atgacgtttg tctttaatca
     1321 caaatccgga gccttgcttc ttgtcttcca tgaattcctt cctccccttt tctgcgaaaa
     1381 atatttaaga ctttttcgtc aacgttattt cgcctttttt ggattgatcg attaaaattt
     1441 tatcgccttc ggcaaatttc cccgccagaa tctccatggc caacgggtcc agaatcagct
     1501 tttgcagcga gcgtttcagc ggccgcgcac cgtaaaccgg actatacccc tgcccggcta
     1561 tatagttttt cgctttttct gtcaattcga cgctcatctt gcgttccttg agacgcttat
     1621 cgatgagacc catctgaata tcaacaatct tgtaaatatc ctccagcttc aacgccgaaa
     1681 acatgataat atcgtcgata cgattcagaa attccggttt gaacgtggcg cgcagcgcat
     1741 ccatcgaccg gtttctcttt tcttcatcat ccagcgacgg atcctgaatc cactgactac
     1801 ccacattgga ggtcataatg accacggtat tcttgaaatc caccgtccgt ccgtgtccat
     1861 cggtcagacg tccgtcatcc agaatctgaa gcaaaatatt gaagacatcg ggatgcgctt
     1921 tttcaatttc atcaaaaagc aaaacgctgt agggtttgcg tcgcacggcc tccgtcagat
     1981 aaccgccttc atcgtatccg acataaccgg gcggagcgcc gatcagacgg gccaccgcgt
     2041 gcttttccat atattccgac atgtcaatac gaatcatggc ctgttcatta tcgaacatga
     2101 attccgccag cgcgcgcgcc agttcggtct tgcccacgcc tgtcggcccg agaaaaatga
     2161 aagaaccgat gggacgattc ggatcctgca gaccggaacg cgcccggcga agagcggccg
     2221 atacggcgtt gataccctct tcctgaccga tgacgcgcaa tttgagacgc tcttccatat
     2281 gaagcagttt ctgaacatca ctttccatca tccgggaaag cggtatcccc gtccagttgg
     2341 aaacgacttc agccacgtct tccgcatcta cttcctcctt gagcatctga ttgtttttct
     2401 gaatatcttc gagttttttc tgctcgttct gaagttcctt atccaacgcc accaatttac
     2461 cgtaacggat ttccgcagcc ttttcgagat tgccatcccg ctgggcgttg gcctcctccg
     2521 tcttcaggga ttctatccgg cttttaatat tttgaatctt tttgatgata tctttttcac
     2581 cagaccagtg tactttcatc tggtccattt tttcctgaag gtcggcaatt tccttttcga
     2641 tcttgtcacg cctctcttta gcggtcttat ccgactcttt cttcaacgac tgaagctcta
     2701 tcttcagttg aatgattctg cggtcgatgg cgtcaatctc tgaaggcatg ctgtccagtt
     2761 ctattctcaa acgcgacgcc gactcatcaa tcagatcgac agccttatcc ggcagaaaac
     2821 ggtcggtgat gtagcgatga gacagcgtcg ctgccgccac aatcgccgaa tctttgatcc
     2881 gcacgccgtg atgaacctcg tagcgttcct tcaatccgcg caggatggca atagtatcct
     2941 cgacggtcgg ctccttgacc agaaccggct ggaaacggcg ctccagcgcc gcgtcttttt
     3001 cgatgtactt gcgatattca ttcaacgttg tcgcgccgac acagcgcagt tcgccgcgcg
     3061 ccagagccgg tttgagcata ttggaagcgt ccatcgaccc ttccgaagcg cccgcgccga
     3121 ccatcgtatg aatttcatcg ataaagagaa taatctcgcc ctcggcgctg atgacttcct
     3181 tcaacacagc cttgagtctt tcttcgaact cgccgcgata tttcgcgccg gccaccagag
     3241 cgccgatgtc caaaccgata acgcgtttgt ttttcagatt ctccggcaca tcaccgttaa
     3301 cgatacgctg cgccagacct tccacaatcg ccgtcttgcc gacacccggt tcgccgatca
     3361 gcaccggatt atttttggtg cgccgtgaga gaacctgcat gatccggcgg atttcgtcgt
     3421 cgcgtccgat caccggatca aaagaacctt ttctggccag cgcgttgaaa tcgcgggcat
     3481 agcgctcgag tgcctgatat tttccttccg gattcggatc ggtcacgcgc tgattgccgc
     3541 gaatatcttt cagcaccttg aaaatattgt ctttggtcac accggcctga cgcagaattt
     3601 ttccggcctg gccttccttt tcctcggcta tacaaaccag cacatgttcg gcgcttacat
     3661 attcatcctt gagctgcgcc gcttcggtca tcgccttgtc gaatatttta ttgagcctgc
     3721 cccccaggta aacttgtccc gcgccggcac cgccgacact cggttgtttt ttcagataac
     3781 cgtcgagttc cttctcgatt ttttgtgatg aagcattgag cttgttgatg atcgccccgg
     3841 caatgccatc ctcctgtcgc aagaaacaaa ccagcagatg ctccggttcg attgactgat
     3901 gccccatgga accggctaat gtttgcgctt cctgaagcgc ttcctgaact ttcaatgtaa
     3961 atttatcaaa acgcatattg tttacctttt atcaaaatct aattttcaaa tctccattcg
     4021 ctttaatgag ccttgatttt ccgaaacgta gtaatcggaa aatcttagag cgaattatcc
     4081 cgccaccttc agttggcagg ggcgagcatc acaaagctcg ctctcagcga gtatccttac
     4141 cctacatcgc ttcgctccta ggatagttcg gcttacgctt cagatttcgt ttcacccgtc
     4201 ttaccacggc actactcgcc tggtataatt cgccctgcaa gcttcagcac acttcgtttc
     4261 tgaagcttgg gtctcatgat cagattgcct cgctgccact tacgcttgct atagtcagcg
     4321 agccttgtta ctcaacgggt ttttcaatgg tcacaaagag cttgccgttt ttgaatatcg
     4381 tcacgtttcc cgaagattcg gaaatgacga tggctacagc tctggtcaaa ctggtgatac
     4441 cggccgcggc aatatgacgg ctgcccagac cgggcgggaa atccttgcta tccagagcgg
     4501 cgctgagatg ccttccggct gtgaccagca cgccgttttc cttgatgata aacgctccgt
     4561 caatggccga gagctccttg atcgtctctt ccaaatccgg attcagaata ttgagctgat
     4621 cttcgggata ccccaaaaat ggattgatga tcatctgtcg ggaaagctgc ataaccttct
     4681 cgtcatcacc cagaataaag atggttccca cctttctgct ttctctgcct tgagcggcca
     4741 gctccagcgc tatattcagc acagcattaa atacttccgg gcgcacattt tccagaacat
     4801 cattgatgaa atccgacgtt agaatctcaa attccttccc tacatcgatc acaaaaatgc
     4861 tgtcggcgta accgaatttc ggcaccccgc ttaaacaaac caccttatcg cctcttttga
     4921 gagcacccag aatgattccc ttggcaatgg caatttttat tattcccacg cgggtcaaat
     4981 tcacattcgg gatataaagc aacttcgcgt cttttttcat ttcttccgat aggctttcgt
     5041 cctcgcggga aaccataatg aaagcgatat ctttttctat ttcatccata atcagactga
     5101 catcactgac cacatcagca cagacaagca gtgcgttggc tttgatttca cgtgcaattt
     5161 tgacggcatg atcaatcatt gttttattta ttgttttcat tgatattcac cttgtttttt
     5221 attgttttta gaataagcat aaaaaacgcc tctgtcaagg ttggatcatt caaaaaaaca
     5281 ctgatttaga tgcatttatc caccggtttc cgatctttct gtcacttgat tcattctgga
     5341 cttttttcaa aaaagaaaac ttgaccgtaa aattatagtt ggctagtatt tataaattac
     5401 cggcaaagga gacgtgacag cattggaatt tcccgtagta accggcacac ttaatttata
     5461 tatagcggaa atcaatcgtt ttcctctgct gacggctgag gaggaattta aactggctgt
     5521 gcgcctgaaa aagtacaacg atgtctccgc tgccgaaaag ctgatcgttt ccaatttgcg
     5581 ttttgtcgtc aaaattgctc atgaataccg aaactacggt ttcaaactgt ctgatttaat
     5641 tcaggaaggc aatatcggcc ttattcatgc cgtaaagaaa ttcgacccgt ataaaggata
     5701 cagactgatt tcttacgcgg tctggtggat tcgcgcctat atccagaatt atcttatccg
     5761 ctcatggagc atcgtcaaaa taggaaccac tcaggctcag cggaagcttt ttttcaaact
     5821 gaatcaaacg aaaaaagaac tggaaaatct ttcccggaaa aatccggagt tcagtgaaat
     5881 tgccacatct ctgggagtta aggattccga agtggaagaa atggacctgc gcatgggagc
     5941 gcgagatgtc tccctgcatg agatcatcaa cgacgaaagc agcaactcgc acatcgattt
     6001 tctggcgtac gaaggagacg atcaggagat ctccctgata aaaaaagagg agaaaagcct
     6061 ggtgcaacgg aacattgccg gagcgctggc caatctcaat gagagagaaa gttacattat
     6121 tctcaaccgc gtcatgtcag acaatccgaa aacgcttcag gagatcgggg aaaaatacaa
     6181 tatttcgcgg gaacgggccc gtcaaataga aaaacaggcc ctgaaaaaaa tgcaacttgc
     6241 attgccatac tttaaaccta acaaataaca caatcgttac aacaggcagg tttttacgaa
     6301 aatatttttc aatattttta taaaaacgct ttagataaga gatggaatcc aaatcatcct
     6361 gcgccataac agggggcaca cattattgga aaaaataatt ttttttaaat actttcatcg
     6421 tttaaaaagc agactggcgg gcaaacatca gaatgaaaaa ctgacggaag acgaactcct
     6481 gcgttcggaa ttattcagcg ccaagcagat gcgacaacac ggcaaaacca ttgccggtgc
     6541 tcataaactg aacgaaggtc tcacctcaaa ccagttgctt gaacgattaa cggaaaatga
     6601 aaaggtgctt ttccaggccc gggaaaaatt gaccaaagcg gtgctggaga accgccgcgt
     6661 aacaccggcg gaagaatggt ttctggataa cttttatctg attgaagagc agatccgaac
     6721 ggcaagacga cacctcccga aagtctacag cagagagctg ccccatctgc tcaacgggcc
     6781 gtcagccaat cttccccgcg tctatgacat agcgctggaa atgatatccc acggcgatgg
     6841 tcgggtggat tcgaaaaccc tgagcctgtt tgtttcttcc tatcaatccg tcaccgtact
     6901 gaaaatcggt gaattatggg caatccctat tatgttgcgc ctggcgctga tcgaaaacat
     6961 ccggcgtatc gcgtcacgta tctccaaaga cagaaccaac cgggacctgg ccgattactg
     7021 ggccagaaaa atgatcgcca ccgttcacaa ggatccaaaa aatctttttc tggaaatcgc
     7081 cgatatggtc cgttcggaac cgccaattgc cggccccttt gttgcggaaa tggcccgtca
     7141 gctgcaggga cagggcccgg ctctggcatt gccgctgacc tggatagagc agcagctttc
     7201 cgaatacggt cagaccatta aaaatttcgt ccagatggaa agtcagcagc aggcggctga
     7261 ccaggtttcc atgagcaaca gtatcgtcag tctgcggttt ctgggaacga tgaactggcg
     7321 ggaattcgtg gaaaccatga gtttcgtgga tcaggtcctg cgcgaggatc ccgcggatat
     7381 ttatgtcaaa atggattttg ccacccgcga ccggtaccgt catgtcatag aaaacatcgc
     7441 aaaaaaaagc agtctatcag aaaccgaagt ggcgcgtatg gcgatattgc tcgcccgtaa
     7501 gacatcaaac cagaagggcg gccaggaccg tacggcacat gtcggtttct atttaatcga
     7561 caacggattg ccggaactcg agcgtctgac caagatgcgt ttctccctct tcgagagcgt
     7621 tgcccgtttc agccggcgat ttccgctgtt gatttatgtc ggttccatct tcctgctgac
     7681 gtttacagcg gcttttcttt tgttagcaat ggcttatgcg gacgggctga ccgggtggct
     7741 gctggctgtt atctggcttg tggcccttct gagtacaagc catttaagca tagcgctgat
     7801 caattgggtg tcgacgcttc tgataacacc cgctccattg ccgagaatgg acttttcccg
     7861 cggcattcct cccgaatacc gcaccctggt ggccattccg acaatgctgc tgaatgataa
     7921 gaacgtcggc aaactgattg aatcgctgga agttcgtttt ctggcgaacc gcgacgaaaa
     7981 tctgcgcttt gcccttctga ccgatttttg cgacgcgaaa accgaaaaaa tgtccgatga
     8041 cggccagctt ctgcgcctcg ttcagaaagg gattgagagc ctcaacgaaa aatacgctga
     8101 tgaccgggga gatatttttt tcctgtttca ccgttcacgc cgctggaacc cgaaagaaaa
     8161 tatctggatg ggttatgagc ggaaaagggg gaaactggct gaactgaacg cctttctgcg
     8221 cggtatcacg cacggccccg aaggagggtt gttctcactg attgtcggcg acacacagga
     8281 attaagaaat gtcaaatacg tcatcactct cgatacagac accaaactgc cgcgcgatgc
     8341 ggcatggcag tttgtgggaa ccatggctca cccgctcaat cgggcgcaat atgacgaaca
     8401 gaaaagacgc gttgtccagg gatacggcat cctgcaaccg agggtctctg tttcgcttcc
     8461 cgatatcagc cggtcgcttt acagcatcat gcacggaagc agttccggaa tcgatcccta
     8521 caccagggcg gtttccgatg tgtatcagga tgttttcggc gaaggttcgt ttattggcaa
     8581 gggaatttac gatatcgatg ctttcgaagc atccctgaac gggcgctttc ccgaaaatta
     8641 tattctcagt catgatctga tcgaaggctg ctatgcgcga tcgggcctca tcagcgatgt
     8701 tcagctctac gaagactatc cgtcagcata caaagccgat gtcagcagaa ggcaccgctg
     8761 gattcgcgga gactggcaga tcgcccgctg gatcatgtcc agggttcctt ccgctgatgg
     8821 ggaaaggctc ggcaatcctc tttccgtgct ttcccagtgg aaaatctttg ataatttgcg
     8881 cagaagcctt gttccggcat cacttacgct catgctgatt ctggggtggc tgctgtcttc
     8941 ctcgcccggc ttgtggacac tggccgtgtt gagtatcatt gtgtttccgg cccttgtcat
     9001 gtcgttgatg agcgtttttc aaaaacagca gaatgtgatt ctgacgcatc acctgtcctc
     9061 agtcttccag aaaaccggca ataatttttt acaggttttg ttttcattta tttgcctgcc
     9121 ctacgaagcc tatttcaaca cggatgcctt gctgcgcacg ctctggagaa tgctgatttc
     9181 gcacaaacgg cttctggaat ggacgccgtc ggaaaacacg aacaatcaac gcgacgatct
     9241 tgccggttat accaggacca tgtggtttgc accgtctctt gccgccgcag tattcgctgc
     9301 attattgaca gcagttccca ccggcgtacc gtccgcggcg ttttgtctta ttctctggct
     9361 gctttccccg gccgtcgcct ggtggttcag ccagccgtcg cgactccggg aagccagact
     9421 ttcttcagcc caaattaatt ttctacgaaa agcatccaga aaaacatggg cgttttttga
     9481 agaattcgcc ggtcccgaag acaactggct tcccccggac aattatcagg aacaccccgt
     9541 ggaagtcgtc gctcaccgca catcgccgac caacatcggc ctgtcgcttc tggcgaacct
     9601 taccgcatat gattccggtt acatcaccgt tttaaaattt attgagagat catccaacac
     9661 cctgcgaacc atgacctccc tggaacgata taaaggtcat ttttacaact ggtatgacac
     9721 gcagtccctg gatccgctgt tgcccctgta cgtctcatcg gtcgacagcg gcaatctcgt
     9781 cggctacctt tttattttaa aatcgggact gtcttccttg cccgataaaa aaatcatcga
     9841 cccgcaaata cttgacggcc tcaaggacac gctgggaatc ctgaatgact ctacgggaaa
     9901 atcagtttcc gcaaatcttt cccaaatcag caagaccctg gatgacgcca cggcttcgcc
     9961 gccggccaaa ctgacggaaa tgaaaatcgt cctggagaaa ataatatcgc tggctgaaga
    10021 aacggcatca tgcatgcgtc cggaaagcga tgcttcaaac gccgccggcc actggttaaa
    10081 ggccttcctt cagcaggcaa cagcggctct tgacgaaatg ctctttctca caccatggct
    10141 gtttgatccg cagttttcgg atctcatccg ggaagtgccc gaactcgatg acatcatgac
    10201 gctgagagaa ttaacaagcc tttatgaaca cgttgaatcg aaaacagagg caatgaaaag
    10261 attcgaatcg accgccattg acctctcgcg aatcagcgac ctgaaaaatc tgattgccga
    10321 atccgggaac cgcgccgtca atttgatgca aaacatcgac ttgctggcgc ggcaatgcac
    10381 ggaattcacc gatatcgaat atgacttcct ttttgataaa tcctgcaatc tcctctctat
    10441 cggttataac gtaacgaatt cccgccgcga tacaagcttc tacgatttgc tggcggcgga
    10501 agccagattc agtaattttg tgggcatcgc gcaggggaaa ctgccgcagg aaagctggtt
    10561 tgccctgggc aggctcttga ccatggctcc cgggaaaaaa cctgtgctcg tttcatggag
    10621 cggctcaatg ttcgagtatc tgatgcccct cctgatcatg ccgacctacg aaggatcgct
    10681 gctcgatcag acctacaaag cggctgtcgc cgcgcatatc gagtacggca aaaaacgctc
    10741 cgtgccctgg ggaatatcgg aatcgggata ctatgctttt gacagtcatt tgaattacca
    10801 gtatcgcgct ttcggcgttc ccggcctcgg tctgaaacgc gggctggcag aagatttggt
    10861 tgtggcacct tacgcttccg ctctggcgtt gatggtggaa ccggagaaag cctgtctcaa
    10921 cctggagaaa cttgcctcca gggaatttca gggcaaattc ggattttatg aagccatcga
    10981 ttttacgccg tcgcgtctga cacggggcca atccaatgct gtcattcgat cctatatggc
    11041 tcatcatcag ggcatgaccc tgctggccct gacttacttg ctgctggacc ggccgatgca
    11101 aaaacgattc gaagccgaac cgctatttca gtccgctcta ttgctccttc aggagatggt
    11161 ccccaaagcc gttgcgtttt atgcatcgcc ttccgaactc atcagcgcac agaaagaacc
    11221 ggaaatctcc gaaaaccctg tacgcgtttt caacacgccg gacacgacgg ttcccgaaat
    11281 acaactgctt tccaacggcc gctatcacat catgctgacc aacgccggcg gaggttacag
    11341 ccggtggaaa aatcttgatg ttacccgttt ccgcgaagac gtcacctgcg acagctccgg
    11401 cattttctgc tatcttagcg acgttaaaac cggcgacatc tggtcaacca cctatcagcc
    11461 gacgcttaaa aaaccccgca attatgaggc aattttctcg gacggccggg tggaattccg
    11521 gcgtcgcgat tatgaatttg acgtgcacac ggaaatcgtc gtttcaccgg aagacgacat
    11581 cgaactccgg cgggtcacga ttaccaacca gtcacgattg caaaaaacaa ttgatatcac
    11641 cagttatgcg gaggtggtca tgacacactc cgctgcggac aagctgcatc ccgcgttctc
    11701 caatcttttt gtccagaccg aaattatcga gcagcgccgg gcgattgtgt gcaccagacg
    11761 gccgcgttcg gaacaggaaa tcaatccctg gatgcttcat tcgatggccg tgcacgggca
    11821 ggacatcgaa caaatctcct atgaaacgga ccggtcccgt tttatcggcc gcggcaatac
    11881 actggcggat ccgcaggcca tgggcccccc cagcgatctt ttcggaactc tttccgacag
    11941 tcaaggctcg gtgcttgatc cgatcgttgc catcagatcc cgcataaccc tcgggccgga
    12001 acaatcggcc gtggtggaca tggttttcgg catcggccaa acccgggacg aagccataac
    12061 gctcgtggac aaatatcagg atcgccgcat tgccgatcgt gtttttgaaa tggcctggac
    12121 gcaaagccag gtgcttctcc gacagatcaa cgcaacggaa gccgatgccc agttgtattc
    12181 ccatatcgcc agttcgctta tttttcccaa cgattccctg cgtgccggcg ccggcgtgat
    12241 ctcggaaaat cgccgggggc agtcgggcct ctggggttac gcgatatccg gcgatctgcc
    12301 gatcgtcctg ctgcgggttt ccgaccagtc ccatatcgaa ctggtgcgcc aactcattca
    12361 ggctcacgcc tattggagac ttaaaggact tgccgtcgat ctcgtaatct ggaatgagga
    12421 gcatgccggt taccgtcagt tgcttcacga tcagatcatg ggtcttgtat cgtcgggctt
    12481 tgtcgccgat ctgaaagatc agcccggcgg aattttcgtc cggtccacgg atcagatgcc
    12541 ggaagaagac cgcattctga ttcagacggt cgctcatgtt attctttccg gcaaccgggg
    12601 caaactggcc aaccaggtga aacagcgcgg cggcttgaaa acggccgcgc cccggatgat
    12661 ccccaatcgt acgcatcggg aaccgccggt tcccaaagcg gcgcccccgc gctctgatct
    12721 ggttttcttc aacggcctgg gcggttttga tccgtccttc agggagtatg tcatcacctc
    12781 gtcaaaatcc cagattaccc ccgcgccatg gatcaatgtt ctggcgaacc cgactttcgg
    12841 caccgttgtt tcggaaagcg gcatgtccta cacctgggcg gacaatgccc acgaattccg
    12901 gcttacaccc tggcacaacg acccggtcag cgacagaagc ggggaagcct tttatattcg
    12961 ggatgatgaa cgcggccact tctggtcacc gatgcccctg ccccatcgcg gtctggaacc
    13021 ttacacaacc cggcacggct tcggctacag tgttttcgaa cacacggagc gatgtattca
    13081 gtctgaggta tggatctaca ccgccctgga tgcatcggta aaattcatcg ttttaaaaat
    13141 caagaattta acggaacgga cgcgccgcat ttccatcaca ggttacatcg aatgggttct
    13201 gggaggcctc agagagaaaa atgccatgca tatcatcacg gaagcggatc atcagagcgg
    13261 agcaattttc gcacgcaatt cttacaacat ggaatttccc aaccgcatct gttttttcaa
    13321 ctccagcgcg tccatacgga ccattaccgg caaccgcatg gaattcctgg gacgaaacgg
    13381 gacgctggcc gatcccgccg tcatgaaacg tcagtatctt tccggaaaag tcggcgccgg
    13441 cctggatccc tgcgccgcca ttcaggttcc tcttgagatg cttgaggggg aagaaaaaga
    13501 ggttgttttt acgctgggca tggggcgcaa tgttactgag gctcgcaaac tggcacagtt
    13561 tttcagcgat ccggcgacgg ccgccgatgc tcttcaggcg gttcagaatt actggagaga
    13621 gaagcttgat gctatccagg ttcaaacacc cgatccgtcc gtcaacgttc ttaccaacgg
    13681 ctggctgctc tatcagaccc tgtcctgccg tctctgggcc cgcacaggct actatcagtc
    13741 gggcggagcc tttggattcc gggatcagtt gcaggatgta atggcgctga tttacacgga
    13801 accgaatttt gcccgggagc aactgcttct ggccgcttcc cgccagtttg aagaaggcga
    13861 cgtccagcac tggtggcatc cccccctggg ccgcggcgtg cgcacgcaat gttccgacga
    13921 ttatctctgg cttcctctga ccacctgctg ctacatttcc cggacgggtg atcagggcat
    13981 cctggatgaa atcatcccgt ttatcaacgg ccggccgctc aaccgggaag aagattccta
    14041 ttatgatctg ccggttcgat cgccggaaaa ggcaaacctc tacgaacact gcctgcgggc
    14101 cattaagaga ggattgcgtt tcggcgaaca cggattgccg ctgatgggat cgggagactg
    14161 gaacgacggg atgaacatgg tcggcattca ggggaaaggc gagagtgtct ggctcggatt
    14221 tttcctctat gacatcctga cacaattcgc gaaaatcgcc agccggcgcg gggatacgtc
    14281 ttttgaagaa tgctgcagga aagaggccct gcaattgcgc ctgaatatcg aacagcacgg
    14341 ctgggacgga ctctggtacc ggcgcgcttt cttcgataac ggttccatgc tcggttccgc
    14401 cagaagcgag gaatgccgga tcgattccat tgcccagagc tggtcggtgt tgtccggagc
    14461 cggcaacggc catcattcgc ggctggccat ggaatcgtta agccagcatc ttattcgccg
    14521 ggatatcggc atcgtccaac tgctgcatcc gccgttcgat aaatccgggt tgaatcccgg
    14581 ttatatcaag ggatacgttc ccggtgtccg tgaaaacggc ggacagtaca cccacggggc
    14641 ggtctgggcg gccatggcgt ttgccgccct gcacgacaac aaccgtgcct gggaaattct
    14701 gacgatgatc aatcccgtca accatgcaaa ttctccggaa tcaatagcca tctacaaggc
    14761 ggagccctac gttgtcgcgg ctgatgttta cgcgttttcg ccgcataccg gacggggcgg
    14821 atggacatgg tacaccggag cggccggctg gatgtacaga ttgatcaccg aatctctgct
    14881 cggcttcaaa aaggaagacg acacattata tattgagccc tgtctgcctg ccgagtgggg
    14941 cggtttcagc atgcactacc gatacaaaaa gacattctat cggatcgttg ttacggtgac
    15001 gccggaaaga aaaaaagaaa agacagtatt gctcgacggt atccggcagg aagaaaaagc
    15061 cgtcttgttg accaatgacg gcatcgatca tcaggttgat gtcatcctgt aatgacctcc
    15121 ataaaattgt cttgccaaaa agaccgcgat ctgcaagaaa tgccgggaaa tttttttatt
    15181 tcacggcgat gacaaattcg gtccggcaga acagatcatc gctgatctgc ctataagagc
    15241 gggagagaaa tcgtagtgat gcgcaaatat attcttccat tgctggcttt gatcctgctt
    15301 ttccgcatcg gctatacctt tttcgatgtc ggcagaggcc tgtcggaagc caccgcggaa
    15361 ggcccttcga ttttttacgg gcgtagcacg gttatcaaaa agggcgtgca tctcgaaaac
    15421 atgcgcttac ccgatcggct gcgcaggctt tcctataaaa aagtagccgg ccctccttac
    15481 gcagccggga ccttctccga ggaacaatcg cgatggcgga tctttctgaa aaacagtccg
    15541 gacagaaatc cttcggaaga taacggtccg gtcgaaatcg tgttgcgtga cggacgggtt
    15601 gatgccatta tatcgtccac cggtaaaaca ctgacgtcca ttcaactgga gccggaggaa
    15661 atcgtccgga tcatcgatga gaagatgccc tcccggcctg cagtcggtct ttccgccatt
    15721 tccccttttc tgcaaaacgc cgttattgca tccgaagacg cgcgttttta ttcacacatg
    15781 ggtatcgatc tgccgtcagt cgtcaaagcc ttcttctccg accccgcaaa acgaaacttt
    15841 cctcaaagca gttccaccat aacacagcaa ctggcaagaa acttctttct ttctccggaa
    15901 aaaacctttg cgcgcaagct gcgagaatcg gaactcgctt tcgtgctgga gctgaaatac
    15961 acaaaaaaac aggttctgga aatgtatctg aacaggatat actggggaca ggacgggtcg
    16021 cggagcatct acggtgcggg aagcgcagcc ggcttttatt tttccaaatc tgcggcacac
    16081 ctttccctgg aagaggcatc cctgctggcc ggtattttgc actccccgaa ccgctacagg
    16141 aatttcaagg ccgcaaaaga aagacgcaat gctgttttgt ccagaatgaa ggatctgaaa
    16201 atgatcagcg acggcgagtt tcagcgggcc tgcaaagcgc cggtgcgcgt gaaactctct
    16261 aaagcgcctg cacaaacctc ctatttcatc gatttcatac agcggatcac aaaagacgat
    16321 ctgggcagcg aaaaacttta ccatcggggc taccgctatt ataccaccat ggatcctgtt
    16381 gtgcaggcga tcgccgatga agccctcgcc gagggcatcc gggaaattga aaggcgtgct
    16441 ttccctgccg gagagaaact tcaggccgca ctggtggccg ttgatcctgc aacaggagaa
    16501 atgatcgcca tggtcggcgg aagaagctac cgggaaacgc aattcaaccg cgcggtcgac
    16561 gcaaaccgtc aatcgggcag cgctttcaaa ccctttgttc tcctggcagc gctttcgctt
    16621 tccctggagc aaggaaagga tatcacgctt tcttcaaccg tttccggaaa accgctttcc
    16681 atcaataccc ccgagggaat gtggagaccc acaaatttcg aaaacaagca ctacgaccgg
    16741 atcaccattc gtaaaatgat cgaagattcc gtcaacacgg ctacaacacg gctggccggc
    16801 gaaatcggtt tcagggaagt actgaaagca gcgcgccggg cgggaatcac aagtccattg
    16861 agcacagttc cttccatggc actgggaagc tttgaagtca cgccgcgcga acttgcctac
    16921 gcttatacca ccattgcgtc gggcggcatc cgctttgatc cttttgcact ttactcggta
    16981 aaaatggcca acggccgtac catcatttcc aaaacggtac ggcgggaaaa agcgtttgat
    17041 ccccgtatca tctatcttgc cggttacgcc atgcagggcg tgctggagcg cggcacggct
    17101 gccgaagcaa aagcattggg catttacttt ccggcggcgg ggaaaaccgg aacaacgaac
    17161 ggcaacaggg attcctggtt tgtggggtac acaccggata tcgtctgcgc cgtatgggtc
    17221 ggatacgact cgggagccga taccgggctg accggcgccc ggggggcgtt gcacatctgg
    17281 tcacgtttca tccgttcttt ctacggccgg tccgcaccgg caaatatcgt tccaccggca
    17341 ggcatagaaa cagccatcat tgatccgcaa tcagggtacc ttgccacaac gtcgtgccct
    17401 caaaaattca aagaagccta tctgaaaggc accgctccca aaaaagcctg tccggatcat
    17461 ccggtaaaac ccgttatgaa aagaatctat aggatgctat agaaaaccac cggcaaaagc
    17521 acggcaacaa aaaagatcgg cttgtgcatg atgtgaggtt gcgtttttcg aggttaaaaa
    17581 cggcaggggg gggaatcgtg aacctttcag caaatttttt attgcgtggc gggaacaagc
    17641 gttgcaatac cggaaacaac agccatttcc gcgctatttt tcgccaagcc ccaattggcg
    17701 tgccccgtta cagcaatggt ataattggaa ccgtctgtcc aggtcacatc ggtatttgtc
    17761 ccttctgaag atgcatagcc ggctttctcc aaagcggcaa ccgtcaacgt cccctccgga
    17821 ttttctgtaa gataggccag agataaagta taagcattct taacatcact gttgagtgta
    17881 gcgacataac ttctttttct gtaggcgata aactgaggaa tggcaatggc cgccaaaata
    17941 ccgataattg cgataacaat catcaattcg attaacgtga aacctttttg cccttcttta
    18001 cggaaaactt ttagcatgca gtacctgttc aatcgacttt atttaatctt aaaactgatg
    18061 aagcggtatc actgataaaa gcaataaata agccaaaggt tcatgtaagt agttttttat
    18121 gatatatcaa ttttacttaa taaaatcagt aagaattatc ggtcaacgtc ccgcggggaa
    18181 aaaaggcaaa caggcccaca aacaaagata atgtgttttt taaccaaaac gacgttaatt
    18241 taactgacct ggttctctta aagccacaat atccggccag cgcttcgggc ggttttcacc
    18301 caaactgttc atccataaaa acactaaaat gccaaaaaac gtgtccggat catcccgtcc
    18361 tgctctcccc tgacgcaaaa cgccacggac ggcacggcac tgggatacgc tcttttggag
    18421 tgccctgata agagatcaga gtgtgacgtt ttttcagatc accataacca gcctcttgat
    18481 ctaaagactc ttcgtttttc tgcggaactt ctgatcagga aaatagtcgc tttccacgct
    18541 gacgtgttta taatgaaaga caaagctttc cgggacaatc attgtcttcg gtttaaccct
    18601 tcggaaaaaa tcttcttccg aaccataaag aggaaatttt tcgttaaaag gccccccttg
    18661 tgtcgacatc tgacgaaaca gagaggcgcg tgcggcaaaa caaaaaccat ttccctttgc
    18721 tgttttacga aaatttttat tctttacttt tgtacaaatc agattgatct catcttgatt
    18781 atcggagggc acgtagtcat cgacaaaatt ggcaatatgt tgtgcgggca catgtccggg
    18841 ggcattggtc acgggaacgg caacggcaaa gccgggagat gctttcagtg aaccaatcaa
    18901 gtgatccgcc cagtaattgg aaaacacaac atcattattg cacaggatga gatagtcttt
    18961 atctttattg agttcaattc ccttattcca taaactggtc aggccgttat tcgttttgtt
    19021 tttaaaaaat ttcacgcccc gatctttata tttatgataa atgccttcta tatcttcgga
    19081 agagctgtca tccagcagcc acagttcgta atcaatgttt ttgttgattg ttgcgtagaa
    19141 ggattccagg gcccgatccg tataatcgat ctgattgaaa ctggtaataa taatgcctac
    19201 cctgggtttt cggaaagact cttttttggg caaccggtat ttgacaaaat ccaggattct
    19261 tctatgtctt attttcaacc gtaaaaggat tcccgacgca tcaacatcac ttaacgcgtg
    19321 agacagaaag cctccgtaat agagaatatt ttcgtgaacc gctttcttca aaggttttac
    19381 aggatgataa agtttgtttt cttcccgtcc gcaccgccag gatgatggcc tgtatttcat
    19441 tgatgttccc gacaaatcac ctttaacatt gggagcagac atggcataaa acctgttttc
    19501 catacccgga atcgtgtatt tgcctcgacc gctgatttcc cggacggtaa aacccttctg
    19561 atgcaacaag gtcggaagcg ccacttcatg atgtccacac catccgtttc tgaatgcgtc
    19621 atgtaaaaaa gacaaagccg cacgggatat gcgatacaga ggattaaacg aacgaagtct
    19681 tttgtccagg gggatatgtt gcctgggatg ttttaatgcc caccacggcc aggaccgctc
    19741 ttccgcgtat gaccggatat gtgaggtcag aaaatcggcg tcatgaacca gaaaagattc
    19801 aaaaaacgac cgccatggac cggtaaatct gacatcgtat tcaataaacc agtaataatc
    19861 gaaatcagga tgatccagat aaaacttaaa taaagggaaa tgcgcatggc ccgggatcat
    19921 cttcttgcct ataacgggat aaccaagatc gcacaggcac tgatcggaat acaaatacgg
    19981 agacagcttt ttaatctttt ccgggacatc ttttcccgtg gtatcataga gaaaaaaggc
    20041 gtctcccata tcgcataccg cccgccggat atgattgaat tcccgaatga ttatttccga
    20101 tgaaagatgg gaaaaaaata aaaaagcctg attggttttg cgcctcatga aatagccatc
    20161 ctgtcagagg tccgatttaa cgcccattgc gaaactcggt aacccggaac ggctgaggtt
    20221 ccgcaagcga tatccagttt tttctttgct gaaaaagttg tttcaacccg aggccgtgag
    20281 aatgataaac acagacgttt ctgtccctga tgacaacaaa tcccttttga agccaataac
    20341 cggcccattc catgtcttcg cctccgagtc cgtaattttc attaaaagga taacgctccc
    20401 acagatcacg ccggatgatc gcgttggtaa aggccaatat ccccatttgc ttttttttca
    20461 ggacacgttt tctcctgaaa taatttttca gaataatctg gcacctgtta aaaataattt
    20521 tttcccaaaa cgaaccatcc ggcaaggccc atacgtcgcc gtaaataccc gcaatgttat
    20581 cggcggtttt aaaattctca attccatcct taagccagtt ctgggaaacg gtcagcgaat
    20641 ggccgctcaa aaagacaaaa tattttgcgg cagacgacct cgaacaacca aaattcaggg
    20701 cattaggata ggaaaactgt tccggtggga tttgaaaaag gtgcacatca tatttaccaa
    20761 taatttccag cgttcgatcc gtcgatcctg aatcgacgac aacaacgtca aaatcacggt
    20821 acgtctgatt gaacaacctg tttagacatt caccaatcca tttttcttca tttttcgttc
    20881 tgataataat ggatgcttcg ggcatggctg agttcgcacc gtggcacatt tttccgactt
    20941 cttactatat ctttccggat aaataaatgg aaaatcctga agtgaactgg tatagtcata
    21001 gtggacacct cgaaaagagt gatatagaga ataaact
//
LOCUS       DAONQZ010000041        20318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_2974_length_20318_cov_17.496916, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000041 DAONQZ010000000
VERSION     DAONQZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20318
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_20318_cov_17.496916"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLR13_07715"
     CDS             complement(<1..265)
                     /locus_tag="PLR13_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQC19261.1"
                     /translation="MMVQKDKLSKILNKQNQLPYQIAEYLAEKIIHMQIKPGERILEA
                     KIAQELGVSRSPVREALHILNEKFLVELLPRRGAVVAGFSIKFI"
     gene            702..1232
                     /locus_tag="PLR13_07720"
     CDS             702..1232
                     /locus_tag="PLR13_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19262.1"
                     /translation="MRSKRMCKIVPPEKWLKTQITRLCLYFLGMGIETAYKNVPQAKR
                     EMDTLPDQFSFCFNVVNGPSMMILKNGHTVRYVGEKEAYHADLELVLKNIEFAYLMMT
                     GRISTPDAVYHNRQFVRGNLAYMMTMIRVLNIAQTLLFPNFLLRFYIKTVPRLTMKTM
                     VNRAKAYANATIGLFA"
     gene            1265..2470
                     /locus_tag="PLR13_07725"
     CDS             1265..2470
                     /locus_tag="PLR13_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQC19263.1"
                     /translation="MQKKEECPKYYEFFNSVKIISGYKALDNIASELDMLGARKPIII
                     TDKGVSQAGLIDHVVNSFGGTQVAIGALYDDTPVDSSIVAVNEIAGIYRQQECDAIIA
                     VGGGSVIDTAKGVNIVISEEADDLMKFTGNNRLTKPMKPFIVVPTTAGTGSEVTMAAV
                     IANPARNVKMQFTSPLILPKVAILDPVMTMSMPPKITAATAMDALTHAVEAYIGPQKN
                     PVSDAFAFSAIELIKANIIDAAKNGKDRDVRLAMANASLLAGISFSNSMVGIVHALAH
                     ATGGLCHVPHGVANAILLPHGLEFYIKKSPDIAARIGKLLRFFMEPEDYAKIPVNERE
                     KNTVAAIRDMNDSLNKICSLPLTLSQAGVTKDKLEAIANAAINDGAIAFCPVEASYED
                     ALEILKAAF"
     gene            2524..4158
                     /locus_tag="PLR13_07730"
     CDS             2524..4158
                     /locus_tag="PLR13_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQC19264.1"
                     /translation="MNAQVSGTAEHQASAAKDQGVKNDVTVCTNPASCEVIAIYPIHT
                     LDDVKNAVINARRAQPAWQAVPLKKKIAYVKKFAQYIQKNSAKLAEIISQDNGKTIFD
                     AMFAEVGPATLATSYYCKNAAEFLADRKLKAGNIMLMNKRSRIVRVPYGVVGIISPWN
                     YPFAIPFSEVVMGLLAGNTVILKAASETQMVGHALKECIDYAGLPEHVFTYINMPGNK
                     AGDALLDAGVDKLFFTGSVPIGKYLMKKASENLTPLVLELGGNDAMIVCEDADLYRAA
                     CGAVWAGMQNAGQSCGGVERIYVDRKIYHEFLAVLKERVEGLRVGDGNCLMTDIGAMT
                     TRKQMRTVQEHIDDALAKGAKIFAQSKCPQNCKGNFIPCTVLTDVDHTMLVMKDETFG
                     PVVGVMPVENMEQAIALANDSNLGLTCSVWSKNRKKAVQIGRRVMAGAVMINDHLMSH
                     GLAETPWGGFKESGIGRTHGDIGFAEMTQPQVIVDDILSAMPFVRRNFWWQPFEKLQY
                     DGMKGLIDLFHGKGFWERFVGLNKLGHAFYRSFVKK"
     gene            4401..7103
                     /gene="fdhF"
                     /locus_tag="PLR13_07735"
     CDS             4401..7103
                     /gene="fdhF"
                     /locus_tag="PLR13_07735"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5463..5465,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQC19265.1"
                     /translation="MSETFLLNGKETPFEQGQTIVEAAKNGGVEIPTMCYLKGSTPTG
                     ACRICCVEVKGARSLVAACSTPVTPNMEVFTETELVHKSRKMVVEFMLTSGKHNCMTC
                     EANGNCRLQDLAYFYKITTPRFEESEQLYPTEDENPFIVRDFSRCILCGRCVQACNEV
                     VVNRAISQGYRGRKSKIVTGGDLPYNHDDSECVFCGQCVQVCPVGALTEKKAKGLGRP
                     WEVQKIRTTCPYCGVGCQQLLHVKDGKIVKVTAVEDAQPNQGRLCVKGRFAYDFIYSG
                     ERLKTPLIREKNGEFREASWDEALDLVANKFKEIIKKHGPDAVAGVSCARSINEDSYQ
                     MQKLFRAVFGTNNIDHCARTUHAPTVAGLATSFGSGAMTNSFADYAKAKMIILVGTNI
                     SEAHPVASTFVKDAVLAGAKLIVIDPRRTDMVDFADVHIPLKVGSDVALINALMNVLI
                     TENLYDKKYVESCTEGFEELKKKVMEYTPEKAAKISGIPADTIRELARTMASIKPAML
                     MYTLGITEHICGVNNVMSCANLQMLLGNVGVELGGVNPQRGQNNVQGACDMGALPNVF
                     PGYQQVANAEARAKFEKAWNVKSLPDKPGMMMPTMMKNLTTGKVKGFYVFGENLANTE
                     PDIKHVEQELQSAEFLVCQDIFPTETTRFAHVILPAAAWSENDGTFASSERRVNRVRT
                     ASVPPGEAKPNWWIFKEIAKRFGHEWASNSAQEIWDNEVSVLAPSMAGIKYARLEGDG
                     LQWPCPTADHPGTPTLHKGGKFTRGLGMLKAIDYIPPAEVPDKEYPMVLSTGRRLYHY
                     HTRTQTGRSEGINDLLGEETADISPADAEAKGIKNGEKILVKSRRGKVEVKARVTEEV
                     PQGLVWMAFHFREACANWLTNPVYDPVTQTAEYKACAVKIEKLK"
     gene            7301..8074
                     /gene="modA"
                     /locus_tag="PLR13_07740"
     CDS             7301..8074
                     /gene="modA"
                     /locus_tag="PLR13_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQC19266.1"
                     /translation="MKKSNFKQVITKILLTVIGVAFFAGLSGQAFAEEKISVAVAANF
                     ISAFKDMGADFEAKTKIKVEGTFSSSGNLYSQITNGAPYDLLLSADEERPAKLNKDGL
                     ADNPFIYARGQAILWSANKEFCKAKTWQDALKNEQIKKLAIANTQTAPYGTAAQKALE
                     KAGMWTDVQSKLVVAQSIAQSFQYASTESVDAGFCALSATASPEGKKGCFYVLNEAPE
                     IIQSACILKRTTNRAAAEKFAEYLRSPAAQEIKVKYGYR"
     gene            8075..8734
                     /gene="modB"
                     /locus_tag="PLR13_07745"
     CDS             8075..8734
                     /gene="modB"
                     /locus_tag="PLR13_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HQC19267.1"
                     /translation="MDYLAIYVTLKLALVTTVVLMVIAAPVAYALAYNRFSGKSFLEA
                     LIYLPMALPPTVIGFYLIILMGPKGFVGKMWQMLTGGSLLFTFIGITMASIIYSIPFA
                     VQPMKAAFSKIDRRLLESAYVLGLSKKATFFRVIIPNSVSGIAAAAILVFLHSIGAFG
                     VLLMVGGSIPGETKVASIAIYEAVEMMNYKAAGLIALSFIPISYAFLLLVNKLNEGAR
                     S"
     gene            8731..9444
                     /locus_tag="PLR13_07750"
     CDS             8731..9444
                     /locus_tag="PLR13_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC19268.1"
                     /translation="MSIEVSFKKKLKYFDVDISFSCAKKNMVVMIGPSGGGKTTIIRM
                     LAGLEKPKEGKVVFGDEVWFDSLRRIDVPPQKRRLGYVFQDYNLFPHLNLFDNAAFAA
                     VDKKEVEDLFGIFRIGHLKKRKPHMVSGGERQRCAICQALARHPRILLLDEPFSALDA
                     ITRRGLREELKNLKGKMSFPIIYVTHDITEALYLADELLPVVDGKIDDQWLQRMMTRE
                     PSSEVALRAAREPRLTLVY"
     gene            9651..10232
                     /locus_tag="PLR13_07755"
     CDS             9651..10232
                     /locus_tag="PLR13_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HQC19269.1"
                     /translation="MNICTYSYEEYIHLVKSFHGTLAPGLLIGGFIVDLAMKNLPEGE
                     FFDAVCETPVCLPDAVQILTPCTIGNGWLSIVNFGRFAVTLYEKYSGRGVRVYLDTEK
                     LNNWPEVRDWYLKKKKKDQQDAEVLFAQIKEAGHQLLSVQKVQVEPEKVRRKKMGPVG
                     VCPVCGEAYPLKHGGKCMNCQGATPYSSVVPVK"
     assembly_gap    10391..10490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10491..11183
                     /locus_tag="PLR13_07760"
     CDS             <10491..11183
                     /locus_tag="PLR13_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HQC19270.1"
                     /translation="RIGLSYVGIKLDNGVAGLAAVLPGSTARGCTVLKEAGTYIGSPA
                     VETLQYLVEAKDALRRAIGVATANALVLPESGKTEDREATTYLNLQPGEKVAMVGLFA
                     PLVERIRSTGATLTVVEKNPERLEVLSAEEKQKALKDCDVAIITATTLLNGTFEETIG
                     LLGRPRSVVLMGPSTPLVPEIFHDTPVTQLGGSIVADAAKALQVISEGGGTPALRHYL
                     RFVNLLLPDKSR"
     gene            complement(11188..11979)
                     /locus_tag="PLR13_07765"
     CDS             complement(11188..11979)
                     /locus_tag="PLR13_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQC19271.1"
                     /translation="MNLFDKDIQISESDHSRFAGTISENWSVNGNPNGGYLMAMIVNA
                     MLRKSDKKATPVVTANYLSRCVPGKIEIHIAEITRSRQFTRFEARLIQEGDEKIRALG
                     TFVSDNNDCAITRYESKAPELAPIEECVPMSVPPSYTLFQNLDLRLDPACAGWMTGGE
                     LTDVSLNKGYFRFRDGRPLDLFSLFLIIDSMPPAAMASQGATAWVPTIELSVNVRNLP
                     KTDRLKCSLRTRFITCGILEADGEVWDEKGNLAAISRQIAQFKKI"
     gene            complement(12060..13070)
                     /locus_tag="PLR13_07770"
     CDS             complement(12060..13070)
                     /locus_tag="PLR13_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC19272.1"
                     /translation="MPDTLKQYAEKLGFPGSETMAKIFAILYDNADSVAVAEALPGSV
                     ADIAARTGLNEERVRQIAQKLLRKGAISRVLAKGDYYRLYPAMIELRDAVVIMPDCPE
                     ELFELWEIIIKEEMPRIVPVFNKLNLTPMLRVIPIEETVENSSRVLDADSARSIIRSA
                     RIITAIPCPCRTQAKHLGKTKDCIAPDGVNLCMQINGFAEAAIDRGVGEVLSTDEALR
                     RLAVAEEAGLVHTVRNNVKEDMFMCNCCPCCCTAMFLHNQAGFDASFAPSRFLAQVDA
                     DTCTGCGACEEKCAFNAITVGDTAVIDRNKCMGCGNCVIVCPVEAIALQEARPPEFIR
                     RT"
     gene            complement(13176..15410)
                     /locus_tag="PLR13_07775"
     CDS             complement(13176..15410)
                     /locus_tag="PLR13_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQC19273.1"
                     /translation="MGTLHKTGCVLCAQNCGLEVDVDNNRIVKVKPDKSNPKSEGYFC
                     RKGMNVAFHQHNADRLKYPLKKVGNHFERITWDQAIDEIAAKLKDIVGQYGPRSFAYM
                     GGGNQSCHLEVAFGVSLMRTLGSQYQYSALAQEFSGAFWVCGRTHGKQYLHDGPDVKN
                     TDVLLIFGWNGMESHQIPQAPRQLQRLSKDPDKLLIVVDPRISETAKIADIHLRIRPG
                     TDALLLKSMIAIILKEGWINKDYMENHTAGFEQVKSLFENFDVRAALKVCELDFDQVY
                     KVTQAYAKRKSSLRYDLGIFMGRHSGLNSYLIVMLQSICGRLCVPGGNIIPGHIVPFG
                     PNSDERNQKTWRTVATNAFPVCGCFPPNVMPEEILSDHPERLRAVLVSGLNPLRSYAD
                     TTAYEKAFQRLDLLVTVELAMTETAALSHYVLPSRSGYESWDGTFFPFTYPEIFFQMR
                     RPIIAPEGEPLEQGEIDLRLADKLGLIPPIPEKLYKTAEESHAAFRQALMEYAMTEPK
                     AMKTMPFILGKTLGKAMGSVHLAALWGMLQVAPQSFQASAARAGFTPGPTLGEEIFRK
                     ILDHPEGLWVGKTDEGNNFAEIKTPDGKINLFIPELLDELKSIDAQREEAALVMPAEF
                     PLVLMAGRHTSMNANTLMRNPAWNEGKRPCTLAMHPDDAAVLNLKDGQPVRVTTEAGS
                     EEIELEITETVRPGHVTMPHGFGLIYNGVKYGANVNRLTKTTNRDQFGTPMHRYVRCR
                     VEAA"
     gene            complement(15461..15883)
                     /locus_tag="PLR13_07780"
     CDS             complement(15461..15883)
                     /locus_tag="PLR13_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQC19274.1"
                     /translation="MQEKDCIFFQLAKANQLASRFLSQKVSELNLTPVQALVLGFLHQ
                     EDQITSAELGKKTGLDSATLTGILDRLEAAEFIERKNNPEDRRSIHIHLTLKGNALSK
                     EAIRVIAGANLEFLQSLTKEEKRHLFDIIKKLRLGAAR"
     gene            16407..19517
                     /gene="fdnG"
                     /locus_tag="PLR13_07785"
     CDS             16407..19517
                     /gene="fdnG"
                     /locus_tag="PLR13_07785"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:16962..16964,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HQC19275.1"
                     /translation="MNVSRRGFLKISGAVAAVSGLGISLKSIAAHARELKIMHAKETT
                     TVCPYCSVGCSIIVSVRDGKVINTEGDPDSPINRGALCSKGGSIYQMAVNENRLGKPL
                     YRAPYAREWKEVEWEWAIDKIAQNVKKSRDASFKKVNAKGEVVNRTEGIASVGSAAMD
                     LEECFTYQKFLRGLGLVYIEHQARIUHSPTVAALGESFGRGAMTNHWIDFKNADVFLI
                     MGSNPASNHPVSFKWIQEAVDKRGAKIICVDPRFTQSAAKAHLYAPLRSGTDIAFLGG
                     MIKYILDNKLYFAEYVKNYTNASFLVNPDLKLPGENNGVFSGLTDGKYDKGTWSYQKG
                     PDDVIKKDPTLSDPHCVFQLLKKHYSRYTPDVVSRITGTPKDKLLEVYKLYASTGKPN
                     KAGVDLYAMGWTQHTVGTQNIRTMCMIQLLLGNIGIAGGGVAAMRGESNVQGSTDHGL
                     LFHIWPGYLGTPTGSLATLKDFIEKRTPKTKEKDSLNWWKNTPKYVAGFLRSMYGSDM
                     TLEDAYEALPKLDDGANYSWLTIFDQMYKGKFTGFFAWGMNPACSGAHSNKVRQALGK
                     VDWMVNVNLFDNETGSFWRGPGMDPSKIKTEVFMLPCASSVEKEGSIANSGRLQQWRY
                     KAIPYYGDSKPDGDIMSEIFFKVKKLYEKQGGPNAAAITKMTWPYGKHEGKEFHYEPR
                     KVAAEINGFFLEDKTVENPTKKGEFKSFKKGDLVPTFAWLQDDGSTSSGCWIYCGSVS
                     EKGILPMRRGQADPTGLGLYSEWAWSWPVNRRIIYNGASVDLNGKPWDPSRAVITWDA
                     KEEKWKGDVPDGAGNPGKGRPPFIMKPDGVASLYGPGLADGPFPEHYEPLECPVEKNL
                     LSPQKNNPAIKRFDKEGVGSDMDVYSGVGTCDPRFPFICSTYRVSEHWQTGVLTRWCP
                     WLAEMQPGMFVEMSQELAKMRGIETGDKCTVSTARGKVVCTAIVTPRFKPFDIDGNEI
                     HEIGIPWHFGWITTKDRPFNKGDKVAEVFTTGDAANLLTPTIGDANTMIPESKAFMAN
                     VVKGVK"
     gene            19519..>20318
                     /locus_tag="PLR13_07790"
     CDS             19519..>20318
                     /locus_tag="PLR13_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC19276.1"
                     /translation="MTEKIKLYDASKCTACRGCQLACKQWNGLKAGQTEHTGTYQNPP
                     SLQHNTYMIIHFQDYVAPEGDVRWIFRKEACMHCTNAACVTVCPSGALYYNKEFGTVG
                     MDREKCIGCKECISACPFEVPQYDKKTEKVFKCDMCETRITNNLTPACVKACPTGALK
                     WLDKEEAVKTAEARVQQLGGDANVYGDKFVGGTHMMYVLKEKPEVYEGIKQDPKVPFS
                     LTVWKIWLKPLSLLGAGGVIGGALLHYILHGPHKPHPEHYDQDTKQGGA"
BASE COUNT         5087 a         4830 c         5389 g         4912 t
ORIGIN      
        1 caatgaattt tatggaaaag cccgctacga cagcgccgcg gcgcggcagt aattccacca
       61 gaaatttttc attgagaatg tgtagtgctt cccggacggg gctgcggctg acaccaagtt
      121 cctgggcgat ctttgcctcc aaaatgcgtt cccccggttt gatttgcatg tgaatgatct
      181 tttcggcaag atattctgcg atttgatagg gtaattgatt ttgtttgttt aatatttttg
      241 ataacttatc tttttgcacc atcatggata gccttgagtc gtttccagaa taagctgtta
      301 gtttgtatat attattatca ttatcacaaa aaatttattt ccgatatgaa taaatcacga
      361 atatcattat gttaataatg atataagaga acccatcttc aaataaaata tcataaaata
      421 aacaataaat tcttaatgat atgaaaacaa aagggttgac aagaccatga ttctgctata
      481 tgtcgattgt cgacaatcga caatgtgcgt gtaagtttcc attccctata aaagatatta
      541 aacattaaaa tcttcaattt cgtaaggaaa tgaggggccc tgaatgtttt tgatatgttt
      601 ttcatatcgc ttctctaatt ttcatttccc gagattgctc aatcattttt tccattcatt
      661 aaactcacaa aacattaaag aaataaaaaa ggggggacgt tatgaggagt aaaagaatgt
      721 gtaagattgt gccgcctgaa aaatggttaa agacacagat taccaggttg tgtctttatt
      781 ttttaggaat gggaattgag acggcatata aaaatgtgcc gcaagcaaaa agggaaatgg
      841 atacgttgcc ggatcaattt tcattttgct ttaacgtcgt caacggtccg tcgatgatga
      901 ttttgaaaaa cgggcacacg gtcaggtatg ttggcgaaaa agaagcttat catgccgatc
      961 tggaactggt gctcaagaat atcgaatttg cctatctgat gatgaccggc agaatcagca
     1021 cgcccgacgc ggtttatcat aaccgtcagt ttgtcagagg aaatctggct tatatgatga
     1081 cgatgatccg ggttctgaac atagcccaga cgctgttgtt tccgaatttt ctcctgcggt
     1141 tttacattaa aacagtcccg cggttaacta tgaagacgat ggtcaaccgc gccaaagcat
     1201 atgccaatgc aacaatagga ttattcgctt aaaagacaat cacttgagga gggatattgt
     1261 gaatatgcaa aagaaagaag aatgcccgaa atactacgaa ttttttaatt ccgtaaaaat
     1321 aatttcaggg tacaaagccc tggacaacat cgcctcggag ctcgatatgc tcggggccag
     1381 aaagcccatc atcattaccg ataaaggcgt aagccaggcg ggattgatcg atcacgtggt
     1441 gaacagtttc ggcggaacgc aggttgcgat tggcgcgttg tatgatgaca cgcccgttga
     1501 ttcttccatt gtggcggtaa acgagattgc cggaatatac cggcagcagg aatgcgatgc
     1561 gatcatcgcc gtcggcggcg gttccgtgat cgacaccgcc aagggagtga atatcgtgat
     1621 ttcagaagag gcggacgatc tgatgaaatt taccggaaac aaccgtctga ccaagccgat
     1681 gaaacctttt atcgtcgtac ccaccacggc gggaaccggt tccgaggtga cgatggcggc
     1741 ggtcatcgcc aatcccgccc ggaatgtcaa aatgcaattt acatctcccc tgattttgcc
     1801 gaaggtggct attctcgatc ccgtgatgac catgagcatg ccgccgaaaa ttactgccgc
     1861 aacggcaatg gacgccctga ctcacgcggt ggaggcctac atcggtccgc agaaaaaccc
     1921 ggtcagcgat gctttcgctt tttccgccat tgaactcatc aaggccaata tcatcgacgc
     1981 ggcgaaaaac ggcaaagaca gggatgtccg gctggcgatg gccaacgcat cgttactcgc
     2041 cggtatatcg ttctccaatt cgatggtggg catcgttcac gcgctcgcgc atgcaaccgg
     2101 cgggctgtgt catgtgcctc acggcgtcgc caacgcgatt cttcttcctc acggtctgga
     2161 gttttatatt aaaaaatcgc cggatatcgc cgcgcgtatc ggcaagctgc tgcggttttt
     2221 catggaaccg gaagattatg caaagattcc ggtaaatgaa agggagaaaa acaccgtggc
     2281 cgcgatacgc gacatgaacg acagtctgaa caagatctgc agcctgccgt tgactctgag
     2341 ccaggctggg gtaacaaaag ataaactgga agcgatagca aatgcggcca tcaacgacgg
     2401 cgctatagcg ttttgtcctg tcgaggcaag ttatgaggat gccctggaaa tattaaaggc
     2461 ggctttttaa cggatcatat aaccggcatg tatttcgatg atcaataaga agggaggaag
     2521 attatgaatg cacaggtttc aggaacggca gaacatcaag catcggctgc aaaagaccag
     2581 ggtgtcaaaa acgatgtaac ggtttgcacc aatcccgcat cgtgcgaagt gatcgccatc
     2641 taccccatcc ataccctgga cgatgtaaaa aatgctgtaa taaatgcccg ccgtgcgcaa
     2701 cccgcgtggc aggctgtgcc tttgaaaaag aagatcgcct atgtgaaaaa atttgcacag
     2761 tacattcaga aaaattcggc aaagctggca gaaatcattt cgcaggataa cggcaaaacc
     2821 atttttgatg ccatgtttgc cgaggtggga cccgcaaccc tggcgacatc atattactgc
     2881 aaaaatgccg ctgaattttt agcggaccgg aaactcaagg cgggcaatat catgctgatg
     2941 aacaagcgca gccgcattgt gcgggtgccg tacggtgtcg tcggtattat ttcgccctgg
     3001 aattacccgt tcgccattcc tttttccgaa gtggtcatgg gattgctcgc cggaaacacg
     3061 gtgattttaa aggctgcatc ggaaacgcag atggtcggcc atgcgcttaa ggagtgcatc
     3121 gactatgccg gtcttcctga acatgttttc acttacatta acatgcccgg caacaaggca
     3181 ggagacgcgt tacttgatgc cggcgtggat aaactgtttt ttaccggttc ggttcccatc
     3241 ggaaaatatt taatgaagaa agcctccgaa aacctgacgc ccctggtgct ggagctgggt
     3301 gggaacgatg cgatgatcgt ttgcgaggat gcggatctgt accgcgctgc gtgcggcgcc
     3361 gtctgggcgg gcatgcaaaa cgcgggacag tcgtgcggcg gtgtggaacg aatctacgtg
     3421 gatagaaaaa tctaccatga atttctggcg gtattgaagg aaagggtgga ggggcttcgt
     3481 gtcggcgacg gcaattgtct gatgacggat atcggcgcca tgacgacccg gaaacagatg
     3541 cggaccgttc aggaacatat cgacgacgct ctggccaaag gagcaaaaat attcgcgcaa
     3601 tccaaatgcc ctcaaaactg caaggggaat tttatcccct gcactgtgct gaccgatgtg
     3661 gaccatacca tgctggtcat gaaggatgaa acattcggtc cggttgtcgg cgttatgccc
     3721 gttgaaaaca tggagcaagc gatagcctta gccaatgatt ccaatctcgg cctgacctgc
     3781 tcggtatggt cgaaaaacag gaaaaaagct gttcagatcg gccgccgggt gatggcgggc
     3841 gccgtcatga tcaacgatca tctgatgagc cacggtcttg ccgaaacacc gtggggagga
     3901 tttaaagaaa gcggcatcgg ccggacgcac ggggatatcg gttttgctga aatgactcag
     3961 cctcaggtta tcgtagacga tatcttgagc gcgatgcctt ttgtgcgcag aaacttctgg
     4021 tggcagccct tcgaaaaatt gcagtacgat ggtatgaaag ggctcatcga cttgtttcat
     4081 ggaaaaggat tctgggaacg ttttgtcgga ttaaataaat taggccatgc tttttacaga
     4141 tcatttgtaa aaaaataagg taataaaaag cgccctgatt tcgaaaaaaa atcggggcgt
     4201 ttttttgtga aaaatgacgc caaaaatgct aaaaaaaaca taacccatct tattgatttt
     4261 atatgtgaat atattaattt aaaatcggtg ttgacagacc ggcggtcact gttgtatgaa
     4321 ccattcctga catatgccat cattggcata tgtcagaggt gacattttta ttatttatta
     4381 ttgaaaaagg tggatgaata atgagtgaaa catttttatt gaacggaaaa gagacgccct
     4441 ttgaacaggg gcagacgatt gtagaagcag cgaaaaacgg cggggtcgaa attcccacga
     4501 tgtgctactt gaaaggctcc actccgaccg gcgcatgccg tatttgttgc gtggaagtca
     4561 agggcgcgcg gtcgctggtg gcggcctgtt ccacgccggt aacgccgaat atggaggtgt
     4621 tcactgaaac cgagctggtt cataaatcaa gaaaaatggt cgtggagttc atgctgacca
     4681 gcggcaaaca taactgcatg acctgtgaag caaacggtaa ttgccgtctg caggatttgg
     4741 catatttcta taaaattacc acaccgcgct ttgaagaaag cgaacagctg tatcccacgg
     4801 aagatgagaa tccttttatc gtccgtgatt tcagccgctg cattctctgc ggacgctgcg
     4861 tacaggcctg taacgaggtt gtcgtaaacc gcgccatttc gcagggctat cgcggtagaa
     4921 aaagcaagat tgttaccggc ggcgatctgc cttacaatca tgacgacagc gaatgcgtat
     4981 tctgcgggca gtgcgtccag gtatgtccgg tcggcgcgct gacggagaaa aaggccaaag
     5041 gtctgggacg cccctgggaa gttcagaaaa tccgcaccac ctgtccgtac tgcggcgtcg
     5101 gttgtcagca gttgctgcat gtcaaagacg gaaagatcgt caaggtcacg gccgtcgaag
     5161 atgcgcaacc caatcaggga cggctttgtg tgaagggccg ctttgcttac gatttcattt
     5221 attccgggga aagattaaaa acgccgctga ttcgagaaaa gaacggcgaa ttccgcgagg
     5281 cgtcgtggga tgaggcgctt gatttggtgg caaataaatt caaagagatc atcaagaaac
     5341 acgggccgga tgccgttgcc ggcgtcagtt gcgcccgcag tatcaacgaa gattcttatc
     5401 agatgcagaa acttttccgc gcggttttcg gtacgaataa tatcgatcac tgcgcacgta
     5461 cctgacacgc tcccaccgtc gccggtctgg cgacttcttt cggttcgggc gcgatgacaa
     5521 attcttttgc cgattacgca aaagccaaaa tgattattct ggtcggcacc aatatatccg
     5581 aggcgcatcc ggtcgcttcg acttttgtga aagacgcggt gctggccggc gccaagttga
     5641 ttgtgattga tccgcggcgg acggatatgg ttgattttgc cgacgtacac attccgctga
     5701 aagtcggttc cgacgtggcg ctgatcaatg ctttgatgaa tgttctcatc acggaaaatc
     5761 tgtacgataa aaaatatgtg gagtcctgta cggagggatt tgaggaattg aagaaaaagg
     5821 tgatggagta cacgccggaa aaagccgcca aaatcagcgg tattcccgcg gacacgattc
     5881 gcgaactggc ccgcacgatg gcttccatca aaccggcgat gctgatgtac acgctgggca
     5941 ttaccgaaca catctgcggc gtcaacaacg ttatgtcctg cgccaatctc cagatgcttt
     6001 taggcaatgt cggcgtggaa ttgggcggtg tcaacccgca gcgcggtcaa aacaacgttc
     6061 agggcgcctg cgatatgggc gcgcttccca atgttttccc cggctatcag caggtagcca
     6121 atgcggaggc ccgggcgaaa ttcgaaaaag cctggaatgt caaatcgctt ccggataaac
     6181 ccggcatgat gatgccgacg atgatgaaaa atttgaccac cggaaaagtc aaaggcttct
     6241 atgtctttgg tgagaatctg gccaatacgg aaccggatat caaacatgtt gaacaagagt
     6301 tgcaatccgc ggaatttctg gtttgccagg atattttccc gacggaaacc actcgctttg
     6361 ctcacgtgat tctgccggct gccgcctgga gcgaaaatga cggaaccttc gccagttcag
     6421 agcggcgcgt caaccgcgtg cgtacggcca gcgtgccccc gggagaggcc aaacccaact
     6481 ggtggatatt caaagaaatt gcaaaacgtt tcggtcacga gtgggcatcc aacagcgctc
     6541 aggaaatatg ggataacgaa gtatccgtgc tggcgccctc tatggcggga atcaagtatg
     6601 cccgtctgga aggtgacggt ctccagtggc cgtgtcctac cgccgatcat ccgggtaccc
     6661 cgactctgca caaaggcggc aagttcaccc gcggtctggg tatgctcaaa gccatcgact
     6721 atatcccgcc ggcggaagtt cccgataagg aatacccgat ggtgctttcc accggccgtc
     6781 gcctgtatca ttatcatacc cggacacaga ccggacgatc cgaggggatc aacgatctgc
     6841 tgggcgagga aacagccgat atttctccgg ccgatgccga ggccaagggg attaaaaacg
     6901 gcgagaagat tctggtgaag tcccgccgcg gcaaggttga ggtcaaagcc agggtaacag
     6961 aagaagttcc gcaagggctt gtctggatgg ctttccattt ccgcgaagct tgcgcgaact
     7021 ggctgaccaa tcccgtttat gatcccgtca cgcagacggc ggaatacaaa gcctgtgcgg
     7081 tgaaaattga gaaactgaaa taaataggga atcaaagtgt cccgctttca aagcgggaca
     7141 ctttttttgt gcagtgatta ttgtccgaga aacaacatcc gattgatctg ttaaaagcca
     7201 tcccgaaaaa caaatttttt attatgttat tattgacata atgttatttt taatatagag
     7261 tcccctcgta tttacatcag aaagagagga tcttttattc atgaaaaaat caaatttcaa
     7321 acaggtcatc acaaaaattt tattgacagt gatcggtgtg gctttttttg cagggctgtc
     7381 cggtcaagcg tttgcggaag aaaaaatttc cgttgcggtt gcggcaaact ttatttcagc
     7441 ctttaaagat atgggggccg atttcgaagc caagaccaaa attaaagttg aaggcacttt
     7501 ttcctcatcc ggaaatcttt acagccagat caccaacggc gcgccctacg atctgttgtt
     7561 atcggccgat gaagaaagac cggccaaact caacaaagac ggcttggcgg ataatccttt
     7621 tatttacgcc agagggcagg cgattttatg gtctgccaat aaggaattct gtaaggcaaa
     7681 aacctggcag gatgccctga aaaatgaaca gatcaaaaaa ctggccatcg ccaatacgca
     7741 gaccgcgcct tacggaaccg cagcgcagaa agcattggaa aaggccggga tgtggaccga
     7801 cgtgcagagc aaactggttg ttgcgcaatc gatcgcacaa tcgtttcagt atgcatcaac
     7861 ggagtcggtt gacgccggtt tctgtgccct gtcggctacg gccagtcctg aagggaagaa
     7921 gggctgtttt tatgtcctga atgaggcgcc ggaaattatt cagtcggcct gcattctgaa
     7981 aagaacaaca aaccgcgctg cggcggagaa atttgcggag tatctgcgat ctcccgcggc
     8041 acaggaaata aaagttaagt acggttatcg gtaagtggat tatcttgcaa tttatgtgac
     8101 attaaaactg gcgctggtga cgactgttgt tttgatggtt atcgcggcgc cggttgctta
     8161 cgctctggcg tacaatcgtt tttccgggaa atcctttctg gaagcactga tttatctgcc
     8221 gatggcgctg cctcccacgg tgatcgggtt ttatctgatc atcctcatgg gaccgaaagg
     8281 ttttgtcggc aaaatgtggc agatgcttac aggcggatcg ctgcttttta cgtttatcgg
     8341 cattacgatg gcgtccatca tttacagtat cccgtttgcc gtgcaaccga tgaaagccgc
     8401 cttttccaag atagaccgcc gtttgctgga atcggcctat gttctcggtt tatccaaaaa
     8461 ggcgacgttt ttccgggtga ttattcccaa ttcggtcagc ggcattgccg ccgccgccat
     8521 tctggttttt ctgcattcca tcggagcatt cggcgttttg ctgatggtgg gcggcagtat
     8581 ccccggcgaa accaaggttg cctcgattgc gatttacgag gccgtggaaa tgatgaatta
     8641 caaggccgcg ggattgatcg cgctgagttt cattcccatc agttacgcct ttttgctgtt
     8701 agtgaataag ctcaatgaag gagcgcgttc atgagtattg aagtttcttt taaaaagaaa
     8761 ttaaaatatt ttgatgtgga catttcattt tcctgcgcga aaaaaaacat ggtcgtgatg
     8821 atcggtccgt ccggcggcgg caaaacaacc atcattcgca tgctggcggg tctggaaaaa
     8881 ccgaaagaag gaaaggttgt attcggcgat gaagtctggt ttgattctct gcgccgcatc
     8941 gatgtcccgc cgcagaagcg gcgcctgggt tatgttttcc aggattataa tctttttccg
     9001 catctgaatc tctttgacaa tgcggcgttt gccgcggtgg ataaaaaaga agttgaagat
     9061 cttttcggga ttttcagaat cggccatttg aaaaaacgca aaccgcacat ggtgtcgggc
     9121 ggcgaacggc agcgctgtgc aatctgccag gcactggcga ggcatccgcg tattctgctt
     9181 ctggatgaac cgttttccgc gctggatgcc attacgcgcc gcggtctgcg cgaagagctg
     9241 aaaaatctga agggcaaaat gtccttcccg attatttatg tgacgcatga cattacagaa
     9301 gccttgtatc tggccgatga acttctgccg gtggtggacg gcaaaatcga cgatcaatgg
     9361 ctgcagcgga tgatgacccg cgagccgtcg tcggaagtcg ccctgcgcgc cgcgcgcgaa
     9421 ccgaggctga cattagtata ctagattagc tttttatgaa tatgtctact gtgtcatttc
     9481 gaagcgaagc gagaaatctt aatgatcaat attcaagata tctccccgga agagttggac
     9541 tatagcctct ggttgatatg acaaatatat cgaagaagcc gctcccgatg aatcgggacc
     9601 gacaaagaca gcgctttgat ttggaaataa tttaaggagc aaaatatttt atgaacatct
     9661 gtacctattc ctacgaagaa tacatccatc tggttaaatc attccatgga acactggcgc
     9721 ccggcctgct gatcggcggt tttatcgttg atctggccat gaagaatctg ccggaaggag
     9781 aattctttga tgccgtttgc gaaacaccgg tctgtctgcc cgatgccgtg cagattctca
     9841 cgccctgcac catcggcaat ggctggctta gtatcgtcaa ctttggccgg tttgccgtga
     9901 cgctttatga aaaatattca ggtaggggtg tgcgggttta tctggatacg gaaaaattga
     9961 ataactggcc ggaagttcgc gactggtatt tgaaaaagaa gaaaaaggat cagcaagacg
    10021 ccgaggtgct ttttgcgcaa atcaaggaag ccgggcatca attgttgagc gttcagaagg
    10081 ttcaggtgga accggaaaaa gtccgccgca aaaaaatggg tccggtgggc gtttgtcctg
    10141 tttgtggcga ggcctatcct ttaaaacacg gcggaaagtg catgaattgt cagggagcga
    10201 ccccgtattc cagcgttgtt ccggtaaaat aatttaacaa tggggaacgt tgctatgcta
    10261 aaaacaggta tcacgcttag tcgtcttttt gaaaaaactg aaccggctgc caggaatcga
    10321 cagattgccg atttacgcat cggcttaagt tatgtcggaa taaaactgga taacggggtg
    10381 gcgggacttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcatcggct
    10501 taagttatgt cggaataaaa ctggataacg gggtggcggg acttgccgcc gtcctgccgg
    10561 ggagtaccgc acgcggctgc acggttttaa aggaagcggg gacatacatc ggatcacccg
    10621 cggttgaaac attacaatat cttgtggagg caaaagacgc tctgcgcagg gcgatcggtg
    10681 tggcgacggc caacgcgctt gttttgcctg aaagcggaaa aaccgaagac cgcgaagcga
    10741 ccacgtacct gaatctccaa cccggtgaaa aggtggccat ggtcgggctg tttgctcctc
    10801 tggtcgaacg catccgatct accggcgcga cactgaccgt ggttgaaaaa aatcccgagc
    10861 gtctggaagt gctctcggca gaggaaaaac aaaaggcgct caaagactgc gatgttgcga
    10921 ttattaccgc aacgactctt ctgaatggta cgtttgaaga aacaatcggc ttgctgggcc
    10981 ggccgagatc ggttgttctg atgggaccct ccacgccgct cgtgcctgaa atcttccacg
    11041 acacgcctgt cacccagttg ggcggttcaa tcgttgccga tgccgcaaaa gcgctgcagg
    11101 ttatttccga aggcggcggc acgccggcgc tcagacacta tcttcgtttt gtgaatctgc
    11161 tcctgcctga caagagccgc taagatttta tatctttttg aactgggcta tctggcgaga
    11221 aatcgcggcg aggttgcctt tttcatccca gacttccccg tcggcttcca gaattccaca
    11281 ggtaatgaaa cgcgtccgga gactgcattt gagccggtcc gttttcggca gatttctgac
    11341 gttcacggaa agctcgatcg tcggaaccca ggcggtggcg ccctgggaag ccatggccgc
    11401 cgggggcatc gaatcaataa tcagaaaaag cgagaagaga tcgagaggcc ggccgtcacg
    11461 aaatctgaaa tagcccttgt tcagagaaac gtccgtcagt tctcctccgg tcatccatcc
    11521 ggcgcatgcg ggatcgagac ggaggtcgag gttttggaaa agcgtgtatg acggcggcac
    11581 agacatcggc acacattcct cgatcggagc aagctcaggg gcttttgact catagcgggt
    11641 tatcgcacaa tcattgttgt ctgaaacgaa tgtgccgagc gccctgattt tctcatcgcc
    11701 ttcctgtata agacgagcct caaaacgggt aaactgccgc gaacgggtaa tttccgcaat
    11761 atgaatctcg atcttgccgg gaacgcatcg ggacagataa ttcgcggtga caaccggcgt
    11821 ggccttcttg tcgctcttcc ggagcatcgc gttgacgatc atggccatga ggtagccccc
    11881 gttggggttg ccgttgaccg accagttttc cgaaatcgtt ccggcaaagc gtgaatgatc
    11941 cgattcgcta atttgtatgt ctttatcaaa aagattcatg aacatccttt tccttaatca
    12001 ggagcattcc ccccggtatc acaactcctt ggccctttac cgcacaaagc gcctcgggat
    12061 caggtcctcc tgatgaattc aggcggcctg gcttcctgaa gggcgatcgc ttctaccggg
    12121 cagacgatga cgcaattgcc gcagcccatg catttgttgc ggtcaatgac ggccgtgtct
    12181 ccgacggtga tggcgttaaa tgcgcacttc tcttcgcacg cgccgcagcc ggtgcaggtg
    12241 tcggcgtcga cctgcgccag aaaacgggag ggcgcaaacg atgcatcgaa acccgcctga
    12301 ttgtggagaa acatggccgt gcaacaacag gggcagcagt tgcacatgaa catatcttcc
    12361 ttgacgttgt tgcgcaccgt atgaaccagg cctgcctcct ccgccacagc cagccgcctt
    12421 aaggcttcat ccgtactcag aacctcgccc acgccgcggt cgatggcggc ctcggcaaag
    12481 ccgttgatct gcatgcagag gttgacgccg tcaggcgcga tacagtcttt cgtcttgccc
    12541 aggtgttttg cctgggtgcg gcacgggcag ggaatggccg tgatgatccg ggcggaacgg
    12601 atgatgcttc tggccgagtc ggcgtcgagg acacgcgaag aattttccac ggtctcctca
    12661 atcggaatga cccggagcat gggtgtcagg ttcagcttgt taaagacggg tacgatcctg
    12721 ggcatttctt ctttgataat gatttcccaa agctcgaaga gctcttcggg gcagtccggc
    12781 atgatgacca ccgcatcccg caactcgatc atggccgggt aaagacggta atagtctccc
    12841 ttggccagca cccggctgat ggctcctttg cgcagcagtt tctgcgcgat ctgacgcacc
    12901 cgttcttcgt tcaggcccgt cctggcggca atgtccgcca cagagcccgg caaggcctcc
    12961 gccacggcga cgctgtccgc attgtcgtaa aggatggcga agatcttcgc catcgtctca
    13021 gatccgggga atccgagttt ttcagcatat tgtttgagcg tgtccggcat attcgtaaaa
    13081 atctccttcc tgttcaagcg accgtccggt cgtttttatc ggttcggcgc tgtgcgttaa
    13141 tgatgatcaa cagcgcttca ttcgccgtct gcgtatcacg cagcctcgac ccggcatcgc
    13201 acataacggt gcatgggggt tccaaactga tcgcggttgg tagttttggt caaacgattc
    13261 acgttggcgc cgtatttgac gccgttgtaa atcagaccga aaccatgagg catcgtgaca
    13321 tgtccggggc gcaccgtctc cgtgatttcc agttcaatct cttcgctgcc cgcttccgtg
    13381 gtgacccgga ccggctgtcc atcctttaaa tttaagactg ctgcgtcgtc gggatgcatg
    13441 gccagcgtgc aggggcgttt gccttcattc caggcgggat tgcgcatcag cgtgttggcg
    13501 ttcatggagg tgtgccgtcc cgccatcaga acgagcggga attcagcggg catgaccagt
    13561 gccgcctctt ccctttgtgc atcaatgctt ttcagttcat ccagcaattc cggaatgaag
    13621 agattgattt tgccgtcggg tgttttgatt tccgcgaagt tattcccttc atcggttttc
    13681 cccacccaga ggccttccgg atgatctaaa attttccgga agatttcttc gcccagcgtt
    13741 ggtcccggcg tgaatccggc tctggcggcg cttgcctgga aggattgcgg agcaacctgt
    13801 aacattcccc atagggcggc gaggtgaacg gaccccatcg ccttgcccag tgttttgccc
    13861 agtataaacg gcatggtttt catcgccttc ggttccgtca tcgcatattc catcagcgcc
    13921 tgtctgaagg cggcatgact ttcttcggct gttttataaa gtttttccgg aatcggcgga
    13981 atcagtccca gcttgtccgc cagtcgcaga tcgatttctc cctgttccag cggttcgccc
    14041 tcgggtgcga tgatcggccg gcgcatctga aagaatatct ccggataggt aaaggggaag
    14101 aaagtgccgt cccaggattc ataaccggat cgggagggta gtacgtaatg ggaaagagcg
    14161 gccgtttcgg tcatggccag ttccaccgtc accagcaggt ccagacgctg aaacgctttt
    14221 tcatacgccg ttgtgtccgc atacgatcgc agcggattca aaccggacac gagaaccgcg
    14281 cgcagtctct cgggatgatc ggagagaatt tcttccggca tgacgttggg cggaaaacat
    14341 ccgcagaccg ggaaggcgtt cgtcgcgacg gttctccagg ttttctgatt tctttcatcg
    14401 ctgttcggac cgaagggcac gatgtgaccg ggaatgatat tgccgccggg aacgcacagc
    14461 ctgccgcaga tggattgaag catgacgatc aggtaggaat tcaggccgct gtgacgtccc
    14521 atgaaaatac cgagatcgta acgcaaagag gatttgcgtt tggcgtacgc ttgggtgacc
    14581 ttataaacct gatcaaaatc aagttcgcag actttgagcg cggcgcggac atcaaaattt
    14641 tcaaacaggg atttgacctg ttcaaagccc gctgtgtgat tttccatgta atctttattg
    14701 atccagcctt ctttcagaat gatggcgatc atggatttga gcaacagggc gtccgtgccg
    14761 gggcgaatcc gcagatggat atccgcgatc ttcgcggttt ctgatatacg gggatcaacg
    14821 acaatcagca gtttgtccgg gtctttggag agacgctgaa gctggcgcgg cgcctgcggg
    14881 atctggtggc tttccatgcc gttccagccg aagatcagca gaacgtccgt attcttgaca
    14941 tccggaccgt cgtgcaggta ttgtttcccg tgcgtgcgtc cgcacaccca gaacgcgccg
    15001 gaaaattcct gtgccagagc gctgtactgg tactgcgagc ccagtgtgcg catcagggag
    15061 acgccgaacg cgacctccag gtggcagctc tggtttcctc cgcccatata ggcaaaggac
    15121 cgggggccgt actggccgac aatatcttta agcttcgcgg caatttcatc aatggcctga
    15181 tcccacgtaa tccgctcgaa gtgattgccg acttttttca aagggtattt caagcggtcg
    15241 gcgttatgct ggtgaaacgc gacattcatg cccttgcggc aaaagtatcc ttcgctttta
    15301 ggattggatt tgtccggttt gaccttgacg atgcggttgt tgtccacgtc gacttccaga
    15361 ccgcagttct gcgcgcacag aacacagccg gttttatgca gggtacccat atctgctcct
    15421 ttcgtccgga gtgtaaccgg atgctttgaa tgcgttttgg tcagcgggca gcgcccagac
    15481 gcagtttttt gataatatcg aacaaatgac gtttttcttc tttcgtcagg ctttgcaaaa
    15541 attcaagatt ggccccggcg atcacgcgga tcgcttcttt gcttaatgca tttcccttaa
    15601 gcgttaaatg aatatgaatg gaacgccggt cttcaggatt gtttttgcgt tcaataaatt
    15661 cagccgcttc cagacggtcc agaatgccgg tgagcgttgc gctgtccagc ccggttttct
    15721 ttccgagttc tgcggatgtg atttgatctt cctgatgcag aaaacccaaa accagcgcct
    15781 gcaccggggt gagattgagt tcggatactt tttgagaaag aaagcggctg gccagttgat
    15841 tggctttggc cagttgaaaa aaaatgcaat ccttttcctg catagggttt attatcctcg
    15901 catacaaaat actcgtatgc gaggataata aggaaaaaga gttttgtcaa gtgcaaagaa
    15961 tcgaatttcc atcgttatga aaatttgcgg tttgaacggt tggggttttt taatcggcac
    16021 agaacatttc cttttcattt cagcggctga ctttaagtca attacctctg aaattatcga
    16081 taaaaattta atttgaccca aagtcattta tgatgaggag tccccatgtc gatctggata
    16141 ggcgttcaat cttacaatac ttttttatta tgtcaaaaat gacataaggc attgaaatta
    16201 ttagtttttt acgttgacaa catctgctgc ttgatgtata aatggcccgt cataaatgcg
    16261 ttattctttg gaaaaaacgt tgtgctcaat ccaaggcctg cttcttccaa aagcttttcc
    16321 tgaaaatgca aattcaagga acaaatcaca caggaaagcc atagcgcaaa aagaatattt
    16381 caatattgat gaaaggagaa aaattcatga acgtcagccg aagaggtttc ttgaaaattt
    16441 caggtgccgt ggcagcggtc agcggattgg gcatcagtct caaatccata gccgcgcatg
    16501 ccagggaact gaaaatcatg catgccaaag aaaccaccac cgtttgtccg tattgttccg
    16561 tcggatgcag catcatcgtg tccgtgcgtg acggcaaggt tatcaacaca gaaggtgatc
    16621 ccgacagccc cattaaccgt ggagcgctgt gcagtaaagg cggttcgatc tatcagatgg
    16681 ctgtcaacga gaaccggctg ggcaaaccgc tttacagagc gccctacgcc agagaatgga
    16741 aagaggtgga atgggaatgg gcgattgaca aaattgccca gaacgtcaaa aaaagccgtg
    16801 acgccagttt taaaaaagtc aatgccaagg gcgaagttgt taatcgcacg gagggcattg
    16861 cctctgtggg aagcgcggcg atggatcttg aagaatgttt cacctatcaa aaattcttaa
    16921 gggggttggg tctggtctat attgaacatc aggcccgtat ctgacacagc ccgactgttg
    16981 cggctctggg agagtcgttc ggacgcggcg caatgaccaa ccactggatt gattttaaga
    17041 atgctgatgt attcttgatc atgggaagca atcccgcttc caatcatcca gtttctttca
    17101 aatggattca ggaagccgtc gataaacgcg gagcgaaaat tatctgcgtt gatccccgct
    17161 tcacccagtc ggctgcgaaa gcgcaccttt acgcaccgct tcgttcagga acggatatcg
    17221 ctttcctggg cggcatgatc aaatatattc tggataataa actttatttt gctgaatacg
    17281 tcaaaaacta caccaacgct tctttcctgg tcaatcccga tctgaaactt cccggagaga
    17341 acaacggggt attttccggt ttgacagacg gcaaatatga taagggtacc tggtcttatc
    17401 agaaaggccc ggatgatgtg atcaagaaag atcctacgct gtcggatccc cactgcgtat
    17461 tccagctttt gaaaaaacat tattcccgtt acacgccgga tgttgtttcc aggattacgg
    17521 gaacacccaa agataaactt cttgaagttt acaaactgta cgcttcaacc ggcaaaccca
    17581 ataaggcggg cgttgatctt tatgccatgg ggtggacgca gcacacggtc ggcacgcaaa
    17641 acatccggac catgtgtatg atccagcttt tgttgggcaa catcggtatc gcgggcggcg
    17701 gggtcgcggc catgcgcggc gagtccaacg ttcagggttc taccgaccac ggtctgcttt
    17761 tccacatctg gccgggctat ctgggaacgc ccaccggttc actggcaact ctgaaagatt
    17821 ttattgagaa acggactccg aaaaccaagg aaaaagattc tttgaactgg tggaagaaca
    17881 cacccaagta tgtcgccggt ttccttcgct ccatgtatgg atcggatatg actctcgaag
    17941 atgcctatga agccctgccc aaacttgatg acggcgccaa ctactcgtgg ctgacgattt
    18001 tcgatcagat gtacaaaggc aagttcaccg gcttctttgc ctggggcatg aatcctgcct
    18061 gttcaggcgc tcattccaat aaggttcgtc aggcattggg caaagtggac tggatggtga
    18121 atgtcaacct gttcgacaat gaaaccggtt ctttctggcg tggtcccgga atggatccgt
    18181 caaaaataaa aacagaagtt ttcatgctgc cgtgcgcgtc ttcggtggag aaagagggca
    18241 gtatcgccaa cagcggacgt ctgcagcagt ggcgttataa agccattccg tattacggcg
    18301 attccaagcc ggatggcgac atcatgagcg aaatcttttt taaggtcaaa aaactttatg
    18361 aaaagcaggg cggtcctaat gctgcggcca tcactaaaat gacgtggccc tacggcaagc
    18421 atgagggcaa ggaattccac tatgaacccc gtaaagtggc cgcggaaatc aacggtttct
    18481 tccttgaaga caaaactgtt gagaatccga ccaagaaggg tgagttcaag tcctttaaga
    18541 aaggcgatct ggttccgacg ttcgcctggc tgcaggatga cggttccact tcatcgggct
    18601 gctggatcta ttgcggctcc gtgagtgaaa aagggattct gcccatgcgt cgcggtcagg
    18661 ccgatccgac aggtctggga ctttattccg aatgggcctg gtcctggccg gtcaatcgcc
    18721 gcatcattta taatggcgcg tcagttgatt tgaacggcaa accctgggat cccagccgcg
    18781 ctgtgattac ttgggatgcc aaagaagaaa aatggaaggg cgatgttccc gatggagccg
    18841 gcaatcccgg caaggggcgt ccgccgttta tcatgaaacc ggacggcgtg gcctctctgt
    18901 atggtcccgg tttggccgac ggtccgttcc ccgagcatta cgaaccgctg gaatgcccgg
    18961 ttgagaagaa tctgctttca ccgcagaaga acaatccggc gatcaagcgt tttgataaag
    19021 aaggtgtcgg ctccgacatg gatgtgtatt ccggcgtcgg aacctgcgat ccccgcttcc
    19081 cgtttatttg ttccacctat cgggtcagtg aacattggca gaccggtgtt ttaacccgct
    19141 ggtgtccgtg gctggcggaa atgcagccgg gcatgtttgt ggaaatgagt caggaactgg
    19201 cgaagatgag aggtattgaa accggagata aatgtacggt aagtacagcg cgcggcaaag
    19261 tggtctgtac ggccattgtg acaccccgat tcaagccttt tgatattgac gggaatgaaa
    19321 tccatgaaat cggtattccc tggcatttcg gttggattac gacgaaagac cgtcccttca
    19381 ataaagggga taaggttgcg gaggtattca ccaccggtga cgcggcgaat ctcttgacgc
    19441 cgacaatcgg tgatgccaac acgatgatac cggagagcaa agccttcatg gcgaatgttg
    19501 taaagggggt gaagtaaaat gactgagaag attaaattat acgatgcttc gaaatgtaca
    19561 gcgtgccgcg gttgccagct ggcctgcaag cagtggaatg gattaaaggc cggacagacg
    19621 gaacatacgg gcacgtatca gaatccgcct tctttgcagc acaatacgta tatgattatt
    19681 cactttcagg attatgtcgc accagaaggc gatgtcagat ggattttccg gaaggaagcc
    19741 tgcatgcatt gtaccaatgc cgcctgcgtg acggtatgtc ccagcggggc tctttactat
    19801 aataaagaat tcggtacggt gggtatggac cgggaaaaat gcatcgggtg caaggaatgt
    19861 atttccgcct gtccctttga agtgccccag tacgataaaa agacagagaa agtttttaaa
    19921 tgcgatatgt gcgaaacaag aattaccaac aatttgactc cggcctgcgt caaggcctgt
    19981 ccgaccggcg ctctgaagtg gctggataag gaagaggccg tcaaaactgc cgaagcccgc
    20041 gtacagcaac tgggcggcga cgccaatgta tacggcgata aattcgtagg cggcacacat
    20101 atgatgtatg tgttgaaaga gaagccggaa gtttacgagg gtatcaaaca ggatccgaaa
    20161 gttccgttct ccctgaccgt atggaagatc tggctcaagc ccttgagcct tttgggagcc
    20221 gggggcgtga tcggcggagc gcttctgcat tacattctgc acggtccgca caagccccat
    20281 ccggagcatt acgatcaaga tacgaagcag ggaggtgc
//
LOCUS       DAONQZ010000042        20090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3024_length_20090_cov_14.955827, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000042 DAONQZ010000000
VERSION     DAONQZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20090
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_20090_cov_14.955827"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLR13_07795"
     CDS             <1..227
                     /locus_tag="PLR13_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19277.1"
                     /translation="LEWHSRGQEFDPPQLHQNLKGGVFVPSLLFFKELQFSKAKCLMV
                     ALAHKTLTLKRKYSMAAFTCPGRYCAPNDT"
     gene            complement(238..1833)
                     /locus_tag="PLR13_07800"
     CDS             complement(238..1833)
                     /locus_tag="PLR13_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQC19278.1"
                     /translation="MLKFFRKHARGWFMIAVIAIIIVVFVLYFGASRGGEHAQVVATV
                     DKKAITVNEVLNEYDKLLEVARLNLKENLTPELIKKMDLKTKAYNDILNREVIIAKAA
                     DLKIQVSDDELRNSILSMPALQTNGMFDERKYHQILRFNRLSAEDFETLQRADLTARK
                     IEFLVREGVKISDKEIYDLYALQNRKINVDFLRLAASDVKHKISPVESELEDYLKRNG
                     NLFRVPEQTKIKYLFFGADTFPADISDADIQDYYSTYQNKYKAKDGKPLPLAGVRNDI
                     INELKRAKGMQTAYTEARKAREEIYQDDNMDAYARKHNLAVRDSDFFMITKPPQDFAS
                     VKNFADALLNMQKGELSKILSTENGYYLLQVVDKKASYIPKLDTIANEVRQRFIETET
                     QLLAEKEAKSILESLKAGESMEKVASAKGLKIGQTGFFQPGNTIPGVGESPEAAEILM
                     TLSAANPYAEKPLFINNVYFILKFRETTRIDDNAFTAEKEIYRKILTAMKQEEAMQTW
                     LEGNKADMIKEKRIKIKKKAEDL"
     gene            2018..3055
                     /locus_tag="PLR13_07805"
     CDS             2018..3055
                     /locus_tag="PLR13_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQC19279.1"
                     /translation="MFFDYVLGKFSNDLAIDLGTANTIVYVKDKGIVLNEPSVVAVHQ
                     DARGMKKVLAVGNEAKNMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILSVHNRK
                     SLVRPRIIISVPSGITQVERRAVRETVESAGAREIYLIEEPMAAAIGAGLPVAEPTSS
                     MVVDIGGGTTEVAVISLAGIVYSKSVRVAGDKIDEAIVQYMKRKHNLLIGERTAEIIK
                     MEIGCAYPFDEIKVGSIKGRDLISGIPKITETNSEEIREAINEQVTFIVDAIKDTLEN
                     SPPELAGDIVDRGIVLTGGGALLANIDILIREETGLPITITDDPLSAVARGAGMALEQ
                     INVLKDIALEV"
     gene            3258..4085
                     /gene="mreC"
                     /locus_tag="PLR13_07810"
     CDS             3258..4085
                     /gene="mreC"
                     /locus_tag="PLR13_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQC19280.1"
                     /translation="MVFLKKYKVIIIIAVLMVLVLVVISYHLKFGDDKSFLRKVALEI
                     SSPVYKIINGSIQGIKDAWLHYFLLVGVQEENKKLKKKMDELQAVIVSYQEGYLEAQR
                     LKKLLAIPDYHRYPFIAARVIGREQATLSRTVLINKGASDGLKNGMPVVAYPGLIGRL
                     TNVSRNVSRVLLFIDENSNVDAIVQRDRIQGIISGAGPRGMILKYIPKTQDVRKGDII
                     VSSGMGGVFPKGWLIGQVINVDRQNDGLFLKIDVAPFTDFSKLEELLILSSAETADE"
     gene            4088..>4503
                     /locus_tag="PLR13_07815"
     CDS             4088..>4503
                     /locus_tag="PLR13_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19281.1"
                     /translation="MFHYLLLLILSLLLIVLQSTVTDIVFSNYFVFEVSLIIIIYAGF
                     RFEMIKGLLLAFYLGFILDLIGGSVPGVLALAYIMIFLCSFFISDLLDTGKNHVIVLF
                     SFICVFLKETMLQTFYFLVFDIRMRPDIFSLILIQS"
     assembly_gap    4504..4603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4604..6313
                     /gene="mrdA"
                     /locus_tag="PLR13_07820"
     CDS             <4604..6313
                     /gene="mrdA"
                     /locus_tag="PLR13_07820"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQC19282.1"
                     /translation="RGLIMDRNGNVLVDNKPSFDVIYIPNKNKANELSVEKLKSLYES
                     QSMVFPFDQAVLKNAKPYLPVKLDKNVSMEKIALVETNALNLPGIYIDVSPIRLYLDG
                     EILAPIIGYTGEISREEMEKSEGKYAYGDISGKHGLERFFDEYIRGRRGAELVEVNVY
                     GREIKNLGRIDPVSGYNMTLTIDADLQRAAWQAIEGKAGSVVALDPRDGAILAMVSSP
                     SFDPNLFNEGISSELWKQLQSNPFAPLSNKAISGQYPPGSTYKLIVAAAALEEGIISP
                     ETKIFCNGFFTLGNRTYRCWKKGGHGHVNLHQAMVGSCDVYFYTVGKMLGVDKIAEYA
                     KRFGLGDLAGVDLPNEKKGLVPTKAWKLKKKKEAWQMGETISISIGQGFNLVTTLQLA
                     NAYAAFANGGTLWRPYLVKKIDAPDGKTYKDFPPVKKGELKLSNTTMEIINDALKAVV
                     NEPGGTGQNAKLAQIEVCGKTGTSQVLGLPENEKARRRKVLSAFHKDHALFASYAPSK
                     SPEIVVAVILENAGGGGAVAAPVARRVLSAYFEGKKKEKSAGKSEKPGESDNRRNLDG
                     DDR"
     gene            6310..7425
                     /gene="rodA"
                     /locus_tag="PLR13_07825"
     CDS             6310..7425
                     /gene="rodA"
                     /locus_tag="PLR13_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQC19283.1"
                     /translation="MIFLKFDRRIFHYFDWILLSFVLGICVMGVLNIYSTGFSAAGGQ
                     SPFYLKQLQWIVVGLLFMTFVFFMDYRVIVEYAYIIYGFSIFLLVLVLIVGSSSHGAQ
                     RWLGGGFFAIQPSELMKVVIILTLARYFDNHKSSEPYTLKELFIPLLIVAVPFVLILK
                     QPDLGTALMLMVIFASIVFFMGVDKKSLIFVFAAGLVLMPAGWFFLKDYQRERLVTFL
                     NPERDPLGSGYHIIQSKIAIGSGEIFGKGFLSGSQTQLKFLPEQQTDFVFSVFAEEWG
                     FIGSMLLIGLFVVVIFWGLKIALQARDLLGTIIAFGVSALIAWEVFINIGMVLGILPV
                     VGIPLPFFSYGGSAMLSLMAAIGLLNNVSARRFILQK"
     gene            7498..7875
                     /locus_tag="PLR13_07830"
     CDS             7498..7875
                     /locus_tag="PLR13_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQC19284.1"
                     /translation="MWEKKKDAEEIKAFLGQGAEFSGKLIFNGSVRIDGKFEGEIFGQ
                     GSLVVGQGATVKANIDTKSVYISGDVQGNIDVKEKINIHSTGKFSGDVRTPVFVMEAG
                     AIFNGMSHMDDVAQQKIEIPKAD"
     gene            8213..8647
                     /locus_tag="PLR13_07835"
     CDS             8213..8647
                     /locus_tag="PLR13_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HQC19285.1"
                     /translation="MVTVIPDYTLLVQGVLFLTLVFLLNILLYKPILSIIDRRKKQLE
                     ESENEIRLFDENAEKKVAEYEEKLKQAKLKASEAKKEVIQEGANQAKNIIDAVRNEIP
                     VMAREFQQKMDKEVEKAKAVLDGNSRQLSLEIAQKILGRPVQ"
     gene            8644..9222
                     /gene="atpF"
                     /locus_tag="PLR13_07840"
     CDS             8644..9222
                     /gene="atpF"
                     /locus_tag="PLR13_07840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQC19286.1"
                     /translation="MKKTISLITGVFVLLVAVGIAFASAEAEGGHHGADWFGLFKKAF
                     NFVVLMGLLYWLLAAKVKEFFVGRRAEIKENLEKAVERKAEAEKKYREYSEKIDKAST
                     EIDGIIEMIKAQGVTEKQKIIEDAERTAKKMKEDAHARIEQEMKKATEQLKSQAVELS
                     VQMAEEILKKSITQDDHAAMVKEYVNKVVIKH"
     gene            9219..9761
                     /locus_tag="PLR13_07845"
     CDS             9219..9761
                     /locus_tag="PLR13_07845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HQC19287.1"
                     /translation="MISNISKRYARAFFDIAKEENQIEPYYKELDQFTLLISRNESLK
                     DFLANPVFEQAVKKGVVDNLIAKLKLSGMTANFIRLLVDKNRIDILEDIVVCYRQLMD
                     EALKKVRVNVKTAFTLSDELRSLISANLEKALGKTVDITVEQDPTLLGGVVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            9765..11279
                     /gene="atpA"
                     /locus_tag="PLR13_07850"
     CDS             9765..11279
                     /gene="atpA"
                     /locus_tag="PLR13_07850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQC19288.1"
                     /translation="METIKAEEISQIISEQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTYIKEGDIVKRTGKIAQVPVGE
                     ALLGRVIDATGKPLDGKGPIETKEFRRIEMVAPGVIQRQPVNQPMYTGLKSIDAMTPI
                     GRGQRELIIGDRQIGKTAIGIDAIIRQKDTGVKCIYVAIGQKKSTVSQIVERLKQHDA
                     LSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDNGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAYPGDIFYNHSRLLERAARVSQELGGGSLTALPVIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFFSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFAQFGSDLDKATQAQLDRGVRLVELLKQPQFKPMSLGQEIVVLFAGTRGFIDKYD
                     VEKVRIYEEQLLSFVESKHPDIMKEIEDKKIISPELEKKMKEIMTAFDSVFVAE"
     gene            11353..12228
                     /gene="atpG"
                     /locus_tag="PLR13_07855"
     CDS             11353..12228
                     /gene="atpG"
                     /locus_tag="PLR13_07855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQC19289.1"
                     /translation="MASLKDIKRKVGAVQKTKQITRAMNMVAASKFKSAQLKMENFRP
                     YAGKFMDVLNSLALRVESMSHPLLAVRDPKRIRVICMTSDRGLCGGFNTNLIKATERF
                     LKEKAGEGKDVSMITVGRKGRDYFRKKANIIAQKTDVLSKFDMTLAVSIADEVINPFI
                     KEEYDELYLIYNQFMNVSIQKPVVVRLFPLPSIGQEIEDEPDKRVDYSYEPSEEVLLH
                     KLLPMYVHVLVYRALLETSAGENGARMAAMDNATSNCQDLISSLTLKMNKARQAAITA
                     ELMDIVGGTEALAKG"
     gene            12298..13707
                     /gene="atpD"
                     /locus_tag="PLR13_07860"
     CDS             12298..13707
                     /gene="atpD"
                     /locus_tag="PLR13_07860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQC19290.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGKLPAILNAITITNSAINDQEDNL
                     VVEVALHLGDNVVRCIAMDITDGLVRGMKAKDTGAPIMVPVGKEGLGRILNVVGRPVD
                     GLGPVNAQTKMPIHRESPTFLEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMINNIAMHHGGISVFCGVGERTREGNDLYREMLESGVIKQAALIYGQMTEP
                     PGARSRVALTGLAAAEYFRDVEGQDVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATDLGGLQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNILGEEHYKVARQVQVTLQKYKDLQDIIAILGMDELSEE
                     DKLTVSRARKIQRFLSQPFFVAAQFTGTEGKFVSVPDTIRGFKEILEGKHDDLPEQAF
                     YMVGGIEEAVEKAKKIGGE"
     gene            13729..14136
                     /locus_tag="PLR13_07865"
     CDS             13729..14136
                     /locus_tag="PLR13_07865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQC19291.1"
                     /translation="MADELLMEIVTPEKMIFSGNIEEVTLPGTEGEFGVLRGHEAFLT
                     SVEIGEMNYVKDGKKSYYAVNTGYVEVTGDKVTVLIETAEKSDQIDKERALKAKDNAE
                     SRLSQMNKDDVEFEIMRAALARAIARINVAEKQ"
     gene            complement(14241..17222)
                     /locus_tag="PLR13_07870"
     CDS             complement(14241..17222)
                     /locus_tag="PLR13_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQC19292.1"
                     /translation="MSEKSDFPEPTLREGDELYGFTVTRIKTFPTIHTTAYEMIHNKT
                     GAKLLHLHCFDRENLYAIGFRTPPYDSTGLPHILEHSVLAGSEKYPLKDVFKELMRST
                     MQTFINAFTYPDKTVYPVASQIKTDFYNLARVYTDLVLHPRMLRETFLQEGHHLEFSV
                     LDDLSSDLIISGIVYNEMKGAYSSPDSLIYKTLQENVYPDSVYSFDSGGDPNVIPSLT
                     YEQFCEFHSKYYSPSNARFFIYGDIPTKDHLVFLSEILKDFNYIEINSAIGSQKRFTQ
                     PRSVVGGYPIGKDESVERKTVVNIAWLMSENTDFETSLILQIISALLVGSAASPLRKA
                     LIDSGLGEDLTPVSGVEADLKQLMFCVGLRNTQRSDAQKIEQLILKTLKDIIDSGYDT
                     QLIEGVLHQVEFHGKEIVRGSYPYGISLMGAVFQTWLYDGDPLIGLDFSQIIENIRRC
                     WADDPLIFQKMTEKWLLNNPHRVLAVMEPDPDFNEKKEAAYREKMDTLKASLCEEELR
                     QINAKAKKLKEFQNQQDPPEAASALPRLNMEDIPKSIEKTPTRNSLIGDVPVWEHDLF
                     TNGIAYVDLAFDLADIPEELQIYLPLLGKMMTGMGAAGLTYEEMAKRIALKMGGLGYN
                     LGAGFTANGDSTWQKMIFSFKALYRNLPEAMSIISDVLCRGDLSHEARMRDLISERKN
                     NLQSAILPSGHLFARMAAGAALTLPAYRDEQWHGRTQFKFVQATAGEFVSSKSALRDK
                     LETLKQTLFNKNNMVINVTAEHQGLEIAKEHLPCLLDRLPASSQAAPEEEPSLNPVFA
                     GISVPAQVSYVAYILKTPCYTDPASPLLMIAAKELSNNYLYKYIRVQGGAYGGMSSFD
                     ASMGLFSFLSYRDPRISETLQVFKNAQTFYSQNEISSPDMEKAIISTISMIDKPSDPA
                     SKGYTAMMRNFAGITDKIRQKFRDDILSATPQKLKKVLSGYFSQAFKSASVAVYSSPE
                     KLDEANRQLEDKLHIESIFEN"
     gene            complement(17289..18131)
                     /locus_tag="PLR13_07875"
     CDS             complement(17289..18131)
                     /locus_tag="PLR13_07875"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQC19293.1"
                     /translation="MPERKSILCPNCRKLISMDEPSCPYCGLQRPGMHNIAGNIGKLF
                     MASNPVMIIIYVNVALYIFSIFLDPQGIFGGSGPFGILAPSNQSLFLLGASGTFPVIG
                     AHRFWTLISASFLHGGIFHILFNMMALYQLGPFIIREFGVHRFINLYIVTGICGFSAS
                     VISGTPFTIGASAAICGLIGAIIYYGKSRGDSYGEAIYKQAMGWVIALVIFGLIFRGI
                     DNWAHAGGLLSGIGFSFLMGYNDNKQETAWNKMLAYACILLTAAVLLWSVVNSLFIGL
                     NISI"
     gene            18297..19454
                     /locus_tag="PLR13_07880"
     CDS             18297..19454
                     /locus_tag="PLR13_07880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQC19294.1"
                     /translation="MIKIESVAFGGSGVGRADNLVVFVPFSAPGDELEIEITQRKKNF
                     LRGRIRNIIKPSPWRTDPVCRYYGVCGGCCYQHIHYAYQLAIKKKQVEDAFRRIGKVD
                     APPVLETVASPRMYHYRGKARYHVGDDPQTGHIGFLDISGGKIVDIEHCAIMQETINE
                     QLSILRENRQNLWHKDELTIWSRYPDVTSEDQTSVVRIVQGKRILAPDDGFFQANEYL
                     TDRLVDEVCRLVRPGKAENAIDAFCGSGLFSIFLSPFAEDITGIEMDKESVKYAKINA
                     ANLNARNVRFLDGNVEIVLREKFLSLGSKIDLIVLDPPRVGCEMTLLKTLMDLQPSNI
                     IYISCNPATQARDIKYLCEGGYHLQSLHPLDMFPQTEHIEVIGYLKRIMAL"
     gene            19556..20032
                     /locus_tag="PLR13_07885"
     CDS             19556..20032
                     /locus_tag="PLR13_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19295.1"
                     /translation="MASKSKDVRIEQLRIFEKKLAVRLQQLEQKGISPEKAQNDAIVK
                     SLKAKIKQTKARIATFDKFVQQTQALAQAKAAKQAAVVEEVKASAEEAKPKAKKKEDG
                     AAKEPKKQPAAEDGAAKKAKKQPADGQEAAPKKAKKQPDEGDDAAPKKKATKKKEE"
BASE COUNT         5394 a         4254 c         5024 g         5318 t
ORIGIN      
        1 cgcttgaatg gcattcaaga ggtcaggagt tcgatcctcc tcagctccac caaaatttaa
       61 agggaggggt gtttgtcccc tcccttctgt tttttaaaga gttgcaattt tcaaaagcga
      121 aatgtttgat ggttgcgctg gcccacaaaa cgctcacatt aaagagaaag tatagtatgg
      181 ccgcgttcac gtgtcccgga cggtattgtg cgccgaacga cacgtgattt tatgtaatta
      241 taaatcttcc gccttctttt taatcttgat ccgcttttcc ttgatcatgt ccgctttgtt
      301 tccttccagc caggtctgca tggcttcttc ctgtttcatc gccgttaata tttttctgta
      361 aatttctttt tccgcagtga aagcattatc gtcgattctg gttgtctccc tgaattttaa
      421 tataaagtag acattattga tgaaaagagg tttttcggcg taagggttgg ccgcggagag
      481 ggtcatcaga atctcggcgg cttcaggact ttctcccacg ccggggatgg tattgccggg
      541 ctgaaaaaat ccggtctgac cgatctttaa tcctttggcg gacgccactt tttccatgct
      601 ttcgccggct ttcaggcttt ccagaatcga tttagcttct ttttcggcaa gaagctgtgt
      661 ttctgtttcg ataaatcgtt gccggacctc attggcgatc gtgtcgagtt tcggaatgta
      721 ggaagctttc ttgtcgacga cctgcaagag ataatagccg ttttcggtcg agagaatttt
      781 gctcagttcg cctttttgca tgttcagcag cgcatcggcg aaattcttga cggaagcgaa
      841 gtcctgcggc ggtttggtga tcatgaaaaa atcggaatca cgtactgcca gattgtgttt
      901 ccgggcgtag gcgtccatat tgtcgtcctg atagatttcc tcgcgggctt ttctggcttc
      961 ggtataggca gtttgcatgc cctttgctct cttcaactcg ttgatgatat cattccggac
     1021 cccggccagt ggcaatggct tgccgtcttt cgctttgtac ttattttgat acgttgaata
     1081 ataatcctga atatcggcat cgctgatatc ggccggaaaa gtgtcagcgc cgaaaaacag
     1141 gtatttgatt tttgtctgtt cggggacgcg aaaaagattg ccgtttcgtt tcagataatc
     1201 ttccagttcc gattcaaccg gcgaaatttt atgctttaca tcactggcag ccaggcgcaa
     1261 aaagtccacg tttattttcc ggttctgcag agcgtataag tcgtaaattt ctttatcgga
     1321 aatttttacg ccttcccgca caagaaattc aatcttgcgg gccgtcaaat cggctctctg
     1381 cagcgtttca aaatcttcgg cggatagcct gttgaagcgt agaatctgat ggtatttgcg
     1441 ttcgtcaaac atgccgtttg tctgcagggc cggcatggac aggatggaat tgcgcagttc
     1501 atcatcggaa acctgaatct ttaaatcagc cgctttggcg ataatcactt ctctgttcag
     1561 gatgtcatta taggctttcg tttttagatc catttttttg atcagttccg gtgtcagatt
     1621 ctctttcagg ttgagcctgg ccacttccag gagtttgtcg tattcgttga gtacttcatt
     1681 gaccgtgatg gcttttttgt ccactgtggc aaccacctgg gcgtgttcgc ctcctctgct
     1741 ggcgccgaag taaagaacga aaaccacgat aatgattgcg atcacagcga tcatgaacca
     1801 gccgcgggcg tgtttgcgaa aaaatttcaa cataaaaatc acctcaaaac gatacaggat
     1861 ttattcattc ggaaattaat ttttgtctta ttattatagt catttacaaa atgcaaatat
     1921 aatttaaaaa tgagttgatt aggtccgtga aatactgtat gaaaaaaatt taaagcctga
     1981 aagtcattat attaatttgt aaattacagg aggcccaatg ttttttgatt atgtgcttgg
     2041 aaaattttcc aacgatctgg cgattgatct tggtacggcg aatacgattg tatacgtcaa
     2101 ggataaggga attgtcttga atgaaccctc cgtggtggct gttcatcagg atgcccgggg
     2161 gatgaaaaaa gtgctggcgg tgggaaatga agcaaaaaat atgctggggc ggacacccgg
     2221 aaatattgtg gccatcagac cgatgcgcga cggcgtcatt gccgattttg acattacgga
     2281 agcgatgctg aaacatttta ttctgagcgt ccacaaccgc aaatcactcg tgaggccccg
     2341 gatcattatt tccgtgcctt cggggatcac gcaggtggag aggcgcgcgg tgcgcgagac
     2401 ggtcgaatcc gcaggggccc gggaaattta tctgattgaa gagccgatgg ctgcggctat
     2461 cggagcgggg ttgcctgttg ccgaaccgac cagttcgatg gttgtcgaca tcggcggcgg
     2521 gacgacggaa gtcgcggtga tttccctggc cggaatcgtt tattcgaaat ccgtgagagt
     2581 cgccggggat aaaatcgacg aggcgattgt tcaatatatg aaacgaaaac ataatctgct
     2641 gatcggtgaa cgaacggcgg aaatcattaa aatggaaatc ggctgtgcgt atccgtttga
     2701 tgagattaaa gtcgggtcca tcaaagggcg ggatctgatt tcaggcattc ccaagattac
     2761 ggaaacgaat tcggaagaaa tacgggaagc catcaacgaa caggtgacct tcattgtcga
     2821 tgccattaaa gatacgttgg agaattcgcc tccggagctt gccggtgata ttgttgaccg
     2881 cggtatcgtc ctgaccggag gcggagcttt gctcgccaat attgacatcc tcatccggga
     2941 agaaacggga ctgcccatca ccattacgga tgatccgctg tcggctgtgg ccagaggtgc
     3001 gggcatggcc cttgaacaaa taaatgttct caaggacatc gcccttgagg tttaaaaatc
     3061 agacctttaa tctgtgtgca gttttttatt acggaatcgt ttcccggcgg ttttgagtga
     3121 tttttacgat gaagtgaaaa aacagcaaga taaatttctt tcatgatctt ttgcgaaagg
     3181 aagaaatttt gtcagtcttg cgtcccggca tattgacata aaatgcgttt tgtaatcatt
     3241 tccccgtcag gttaaaaatg gtttttttaa aaaaatacaa ggtcatcatt atcattgccg
     3301 tccttatggt tttagtcctg gttgtgattt cataccatct gaaattcggc gatgacaaaa
     3361 gttttttaag aaaagttgcg ctggaaatat cttctccggt gtataaaatc atcaacggtt
     3421 ccattcaggg catcaaggat gcatggcttc actatttctt gctagtcggc gttcaggaag
     3481 aaaataaaaa attaaaaaaa aagatggatg aactccaggc ggttattgtt tcctatcagg
     3541 aaggttatct ggaagcgcag cgcctgaaga agcttttagc aattcccgat tatcaccgat
     3601 atcctttcat tgccgccagg gtgatcggca gagagcaggc cacgctgtcc agaactgttc
     3661 tgatcaataa aggtgcatcc gacggtttga aaaacggtat gccggtcgtt gcctatccag
     3721 ggctgatcgg ccgtcttaca aatgtctccc ggaatgtctc ccgggttctt ttgtttatcg
     3781 acgaaaacag caatgttgac gctatcgtgc agcgggatcg cattcaggga attatcagtg
     3841 gcgccggtcc ccggggaatg atattaaagt atatacccaa gacacaggat gtccggaaag
     3901 gggatatcat tgtttcttca ggcatgggag gtgtttttcc caaaggatgg ttgatcggcc
     3961 aggtcatcaa tgtggaccgg cagaatgatg gcctgttttt aaaaattgat gttgctccgt
     4021 ttacggattt ttccaagctg gaggaattgt taattttatc ttccgctgaa acggccgatg
     4081 aataatcatg tttcattatt tgctgcttct gattttatcc ctactgctta tcgtgctgca
     4141 gtccactgtt accgatattg tgttttcaaa ttattttgtt tttgaagtat ctctgatcat
     4201 tatcatttac gcaggattcc gttttgaaat gatcaaaggg ttgttgctgg ccttttacct
     4261 cggatttatt ctggatttaa tcggcggttc ggttcccggg gtgcttgctt tggcgtatat
     4321 catgattttc ctgtgttcat ttttcatatc cgatttgctt gataccggga aaaatcatgt
     4381 cattgtttta ttcagtttca tttgtgtttt cctgaaagag acgatgctcc agacctttta
     4441 ttttctggtc tttgatatca ggatgcgccc tgatatattt tctctgattc tgatacagtc
     4501 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggggtc tgatcatgga
     4621 ccgtaacggc aatgttctgg tcgacaacaa accgtctttc gacgtgattt atattcccaa
     4681 caaaaacaag gccaatgaat tgtcagtgga gaaattaaaa tcgctttatg aaagtcaatc
     4741 gatggttttc ccgtttgatc aagccgttct gaaaaacgca aaaccgtatt tgcctgtaaa
     4801 gttggacaag aacgtcagta tggaaaaaat agccctggtg gaaaccaacg cgttaaatct
     4861 gcccgggatt tacattgatg tctcgccgat tcgtttgtat cttgacggag aaatcctggc
     4921 gccgatcatc ggttacaccg gtgaaatcag cagggaagaa atggaaaaaa gcgagggcaa
     4981 gtatgcttac ggcgatatat ccggaaaaca tggtttggaa cgatttttcg acgaatatat
     5041 tcgcggccgc cgcggtgcag aactggttga agtcaatgtt tacgggagag aaataaaaaa
     5101 cctgggccgc atcgatcccg tctccggcta taacatgact ctgacaatcg atgcggactt
     5161 gcaaagagcg gcatggcagg ccattgaagg gaaagcgggt tccgttgtcg cactggatcc
     5221 gcgcgatggc gccatcctgg ccatggtgag ttccccttcg tttgatccaa atttatttaa
     5281 tgaaggtatt tccagcgaac tctggaaaca gttgcagagc aatccgtttg ccccgttgtc
     5341 caataaagcg atctccggac aataccctcc gggatcaacc tataaattaa ttgttgccgc
     5401 agctgctttg gaagaaggta ttatctcgcc cgagacgaaa attttctgca acggcttttt
     5461 tactctggga aacagaacat atcgctgctg gaaaaagggc ggtcacggtc atgtcaatct
     5521 tcatcaggcc atggtcggat catgtgatgt ttacttttat accgtgggca aaatgctggg
     5581 cgtggacaaa atagccgaat acgcaaaacg gttcggtctg ggagatctgg cgggtgtcga
     5641 tttacctaat gagaagaaag gactggtgcc gaccaaggca tggaagttga aaaagaaaaa
     5701 agaggcctgg cagatgggcg aaaccatatc gatatccatc ggacaggggt ttaatctggt
     5761 tacaacttta caactggcca atgcctacgc tgcttttgcc aatggaggca ccctctggcg
     5821 tccgtatctg gtcaagaaaa tagatgctcc cgacggtaaa acctataagg atttcccgcc
     5881 cgtgaaaaag ggggagttga aactgagcaa caccacgatg gaaattataa atgatgcttt
     5941 aaaggccgtg gtcaatgagc ccggaggcac ggggcagaat gcgaaactgg cacagataga
     6001 ggtatgcggt aaaacaggga catcccaggt gttgggattg ccggaaaatg aaaaggcgcg
     6061 acgccgcaag gtcctgagcg cctttcacaa agaccatgcg ctgttcgcca gttatgcgcc
     6121 gtcgaaaagt ccggaaatcg ttgtggccgt catcctcgaa aatgccggag gcggcggagc
     6181 cgttgccgca ccggtcgcgc gcagagtatt atccgcttat tttgaaggga agaaaaaaga
     6241 aaaaagcgcc gggaagagtg aaaagcccgg cgaatctgac aaccgcagaa atcttgatgg
     6301 ggacgacaga tgatttttct taaattcgat cgccgtattt ttcattattt tgactggatt
     6361 ctgttgtctt ttgtgctcgg catctgcgtg atgggagttt tgaatatcta ctccaccggt
     6421 ttcagtgcag caggaggcca gtcgcctttt tacctgaagc aactgcaatg gattgtggtg
     6481 ggattgcttt tcatgacgtt tgtgtttttc atggattacc gggtaattgt cgagtatgcc
     6541 tacatcattt acggtttttc catttttctc ctggttctgg ttttaatcgt cggttcgtcg
     6601 tcgcatggcg cccagaggtg gctaggcggc ggtttctttg ccattcagcc ttccgagttg
     6661 atgaaagtgg tcatcatcct taccctggcg cgttatttcg acaaccacaa atccagcgag
     6721 ccttacacgt taaaggagct tttcattccg ctcttgattg tcgctgttcc tttcgtgctg
     6781 attttaaaac agccggatct gggaacggct ctgatgctga tggtcatctt tgcttccata
     6841 gtgtttttca tgggtgtgga taagaaatct ctgatttttg tttttgccgc cggtttggtc
     6901 ttaatgccgg ctggctggtt tttcctcaag gactatcaac gggaaaggct tgtgacgttt
     6961 ctcaatccgg aaagagatcc actgggctcc ggttatcata tcatccaatc gaaaattgcc
     7021 attggctccg gagaaatttt cggcaagggt tttttaagcg gttcccaaac ccagttgaag
     7081 ttccttcccg agcagcagac cgattttgtt ttttccgttt ttgccgagga gtggggattc
     7141 atcggttcca tgttgctgat cggcctgttt gtggtggtca tattctgggg attaaaaatt
     7201 gccttgcaag ccagagattt attaggcaca attatagcgt tcggtgtcag cgcgctgatt
     7261 gcgtgggaag tctttatcaa tataggaatg gtgctgggca tcctgcccgt tgtcggcatt
     7321 ccccttcctt ttttcagtta tgggggatcc gccatgcttt cgctgatggc cgccatcgga
     7381 ttgctgaaca atgtcagcgc gcgccggttt atcctgcaaa agtaaggagt catcgagtat
     7441 aaagagaaaa tcattttgag ccgaatcgat taacataagt atcaaaaggg ggacgatgtg
     7501 tgggaaaaaa agaaagatgc tgaagaaata aaagccttcc tcggtcaggg agccgagttt
     7561 tcaggaaaac ttatttttaa cggttccgtt cgcatagacg gaaaattcga aggagaaatt
     7621 ttcggacagg gatcactggt tgtcggtcag ggcgcaaccg tcaaggccaa catcgatacc
     7681 aaaagtgttt atatcagcgg cgacgtgcag ggcaacatcg atgtaaaaga aaaaatcaat
     7741 attcactcaa cagggaaatt ttccggagat gtgcgaaccc ctgtgtttgt tatggaagcg
     7801 ggcgccatct ttaatgggat gtcccatatg gatgacgttg cgcagcagaa aattgaaata
     7861 cccaaggcgg attgattttg tgcaaaccat cagtattatt tgggtcttga cgtcaaacgc
     7921 ataaaaaaca gcctgatagc attttaattc cccaagtctt tgatattatt aattattgca
     7981 gaaaaacaga ctgatggtca gaaaatactt gacaagccca taatgatgtg attagtaact
     8041 cgcaggtttt gagtatctgg aaattagatt ccatcctttt taggtaaaaa tattcaaaac
     8101 acaactgaat aaaaactgta tgcacttgct taggcatgcc atcaaaaacc gtccataaag
     8161 gaaaatcttc cagcggtttt acaaagtgca aaagataagc atgaggtaaa aaatggttac
     8221 agtcattccc gattacacgt tgttagttca aggcgtcctt ttcctgacct tagttttttt
     8281 actcaacatc cttctttaca aacccatcct ttccattatt gaccggcgca aaaaacagct
     8341 tgaagaatca gagaacgaga tcaggctttt tgatgaaaac gccgagaaaa aagttgcaga
     8401 gtatgaggaa aaattaaagc aggccaagct caaagcgtcc gaggctaaaa aagaggtcat
     8461 acaggaaggt gcgaatcagg caaaaaatat tattgatgcc gtgcgcaatg aaattccggt
     8521 aatggcccgc gagttccagc agaagatgga taaggaagtg gaaaaggcaa aagcggttct
     8581 ggacggcaat tcccgtcagt tatcccttga gatcgcccag aaaatattgg ggaggccggt
     8641 acaatgaaaa aaacaatatc tctgataacg ggtgtctttg tattgctggt tgctgtgggt
     8701 attgcatttg cttccgctga ggctgaaggg gggcaccacg gtgcagactg gttcggtttg
     8761 ttcaagaagg ctttcaactt tgtcgtttta atgggtcttc tctattggct tctggctgcc
     8821 aaggtgaaag aatttttcgt cggtcgccgg gcggagatta aggaaaatct ggagaaggcg
     8881 gtggagagaa aagccgaagc cgagaaaaag tatcgtgaat attcggaaaa aattgacaag
     8941 gcgtcaacag aaatagacgg tatcattgag atgatcaagg cgcagggcgt tacggagaaa
     9001 cagaagatta tcgaggatgc cgagagaaca gccaaaaaga tgaaagaaga tgctcacgcc
     9061 cgcatcgagc aggaaatgaa aaaagctaca gaacagctga aatcccaggc tgttgaactt
     9121 tctgtgcaaa tggcggaaga aattctgaag aaaagcatta cacaggatga tcacgcagcc
     9181 atggtgaaag aatacgtaaa taaggtggta atcaagcatt gatcagcaat atttccaaaa
     9241 gatatgcgcg tgcctttttt gacattgcga aagaggaaaa ccagattgag ccttattaca
     9301 aggaactcga tcagttcaca ttactcattt ctcggaatga atctttaaaa gatttcctgg
     9361 caaatcctgt ctttgaacag gcagttaaaa aaggtgttgt ggacaatctc attgccaagt
     9421 tgaagttgtc cggcatgacg gccaacttta tcaggctgct ggtggacaag aatcgaatcg
     9481 atattttaga ggatatcgtc gtttgttatc gccagttaat ggacgaagcg ctgaagaagg
     9541 tgcgggtgaa tgtaaaaacc gctttcactc tttcagatga gttgcgttct ttaatcagtg
     9601 caaacctgga aaaagcgctg ggtaaaacag ttgatataac ggtggaacag gaccccaccc
     9661 ttttgggtgg cgtggtcatt ggcgttggcg atacacttta cgatggcagt ataaaaaatc
     9721 agttgaacaa tatgaggaat ctcttagggg aggcaagata ggacatggaa acaattaaag
     9781 cggaagaaat tagtcaaatt atttctgagc agatcaaaag ctatgagaaa aagctggata
     9841 taagcgaaac cggaacggtc ttgtcggtcg gtgacggtat tgcccgcgta tacggcgttc
     9901 aaaatgccat ggcgatggag cttttggaat ttcccggcgg aatcttaggt atggtcctta
     9961 atctggaagc ggacaatgtc ggcgtcgcgg ttcttggtga agttacctac attaaagagg
    10021 gtgacatcgt aaagcgaacc ggtaaaattg cacaggttcc cgtcggtgaa gcactgctgg
    10081 gacgtgtaat tgatgcaacc ggaaaaccct tggatggcaa ggggccgatt gaaaccaaag
    10141 agtttcgccg aattgaaatg gtggcccccg gtgttattca gcgccagccg gttaatcagc
    10201 cgatgtacac aggtttgaaa tcgatcgatg cgatgacgcc gatcggacgc ggtcagcgcg
    10261 aactgatcat tggcgaccgc cagataggca aaacagccat cggtattgac gccattatcc
    10321 gtcagaaaga taccggtgtg aaatgtatct atgttgccat cggtcagaaa aaatctacag
    10381 tgtctcaaat tgtggagaga ctcaagcagc atgatgcgct atcttatacc tgcgttgttg
    10441 ccggctgtgc atccgatccc gcgacgcttc aatacattgc cgcttatgcc ggttgcagta
    10501 tcggtgaata cttccgagac aacggtcagg atgctttgat tatttacgat gatttgtcca
    10561 agcaggcggt cgcttaccgt cagatttctc tgctgctgcg tcgtcctccc ggacgtgaag
    10621 cttatcccgg tgacattttc tacaatcact cccgtttgct cgagcgtgcc gcgcgtgtca
    10681 gccaggaatt aggcggtggt tctttgacgg cccttccggt cattgaaact caggctggtg
    10741 acgtttccgc ttacattccg accaacgtta tttcgattac ggacggtcag gtttatctgg
    10801 agccgagttt gttcttctcc ggtattcgcc cggccattaa cgtcggtctc tccgtttcgc
    10861 gcgtcggcgg cgcggctcaa gtaaaggcga tgaagcaggt tgctggaacg ctgaaactcg
    10921 acttggctca gtaccgtgaa ctggcggctt ttgcccagtt cggatccgat ctggataaag
    10981 cgactcaggc tcagttggat cgcggtgtcc gcctggtgga actgctcaaa caaccccagt
    11041 tcaagccgat gtctttagga caggaaatcg ttgttctctt cgcgggaacc aggggtttca
    11101 ttgataaata cgatgtcgaa aaggttcgta tatacgaaga gcagcttttg agttttgttg
    11161 aaagcaaaca tcccgacatt atgaaagaaa tagaagataa gaagataatc tctcccgaac
    11221 tcgaaaagaa gatgaaggaa attatgaccg cttttgattc tgtttttgtg gcggaatagg
    11281 tagagatatt aacggttttg tcaggaaata gacagtcctg aagttcaaat aataagttga
    11341 ggagaattgt aggtggcctc gctaaaagac attaaaagaa aagtcggcgc cgttcaaaag
    11401 acaaaacaga ttacgcgcgc catgaacatg gttgccgcat caaaatttaa gtcggcgcag
    11461 ttgaaaatgg aaaatttccg tccctatgcc ggaaaattca tggatgtgct taacagtctt
    11521 gccctgcgcg tagaaagcat gtcacatcca ttactggccg tgcgcgaccc gaaaagaatt
    11581 cgtgtgattt gcatgacatc ggatcgcggc ctttgcggag ggtttaatac caacctgatc
    11641 aaagcgactg aaagatttct gaaagaaaaa gcgggcgaag ggaaagatgt ttccatgatt
    11701 accgtggggc gcaagggcag ggattatttc cgcaaaaaag ccaacatcat tgcccagaag
    11761 acggatgttt tgagtaaatt tgacatgacg ctggcggttt ccatcgccga tgaagtcatt
    11821 aatcctttta ttaaagaaga gtatgacgaa ctttatttga tttataatca gtttatgaat
    11881 gtttccattc agaaaccggt tgtggtcaga ttattccccc tgccatccat cggccaggaa
    11941 attgaggatg aaccggataa acgggttgat tattcctatg aaccatcgga agaggtctta
    12001 ctgcataaat tactcccgat gtacgtgcac gtacttgttt atcgcgctct gctggaaacg
    12061 tcagccggtg aaaacggcgc gcgaatggcg gctatggata atgcgacaag taactgccag
    12121 gatttgatca gttcattaac gttgaaaatg aataaagcca gacaggccgc gatcacggcg
    12181 gagttgatgg atatcgtcgg cggtacagag gctctggcga aaggctaaat attaattttt
    12241 ctgcggctgt ggtcgcatgt aaaataaatt aatacgacta tttgcaagga gatagttatg
    12301 aatatcggaa aaatcgttca agttattgga cctgtagttg acgtggcttt tgaggaaggc
    12361 aaactccccg caattttaaa cgcaattacc attaccaatt ccgccatcaa tgatcaggaa
    12421 gataatttgg tcgttgaagt tgcgttgcat ttaggtgaca atgttgtccg ctgcatcgcc
    12481 atggatatta ccgacggttt ggttcgtggc atgaaagcaa aagataccgg tgctcctatt
    12541 atggtgccgg tcggtaaaga gggcttgggc agaattctga acgtcgtcgg tcgtcccgtg
    12601 gatggtctcg ggccggttaa cgcgcagacg aaaatgccga ttcaccggga atcgcccaca
    12661 ttcctggagc aggatacatc cgtgcacgtt ctggaaacgg gcgtcaaggt tatcgatctg
    12721 ctggtgccgt ttccccgcgg cggtaaaatg ggcatgttcg gcggcgccgg tgtcggcaag
    12781 accgtcgtta tgatggaaat gattaacaac atcgccatgc accacggcgg tatttccgtg
    12841 ttctgcggcg tgggtgaaag aacccgtgaa ggtaacgacc tgtaccgcga aatgctggaa
    12901 tccggcgtta ttaaacaggc cgctctgatt tacggccaga tgacggaacc ccccggagcc
    12961 cgttcccgtg ttgctttgac cggattggcg gctgccgaat atttccgtga tgtggaaggt
    13021 caggacgtgc tcttctttgt tgataatatc ttccgtttca cccaggccgg ttcagaagtt
    13081 tcggctctgc tgggacgtat gccttcggcg gtcggttatc agccgacact ggccacggac
    13141 ctcggcggac ttcaggaacg gattacctcg acaaccaaag gttctattac agccgttcag
    13201 tgcgtgtacg tgcctgccga cgacttgacc gaccccgctc ccgcgacgac attcgcgcat
    13261 cttgacggaa ccgtcgtttt gtcccgtccg atcgcggaac tcggtatcta tcccgcggtg
    13321 gatcctctgg attcaacatc ccgtattctt gatcccaaca ttctgggtga ggaacattac
    13381 aaggtagcac gtcaggtaca ggtgacgctg cagaaatata aggaccttca ggacattatc
    13441 gccattttgg gtatggatga actttctgag gaagataaac tgacggttag ccgcgccaga
    13501 aagattcaga gattcctgtc ccagccgttc ttcgtcgccg ctcagttcac cggtacggaa
    13561 ggcaagtttg tttccgtgcc cgataccatc agaggcttca aagaaatcct ggaaggcaaa
    13621 catgacgatc tgcccgaaca ggccttttac atggtcggcg gcattgaaga agccgttgaa
    13681 aaagccaaaa agattggcgg cgaataatta ttgatctgga gaatgtaaat ggcagatgaa
    13741 ttattgatgg aaattgtaac gccggaaaag atgatttttt cgggcaatat cgaagaagtt
    13801 acccttccgg gaacggaagg cgagttcggc gtattgcgcg gtcatgaggc ttttttaacg
    13861 tccgtggaaa tcggcgagat gaattatgtc aaggacggca aaaagtcgta ttatgcggtg
    13921 aataccggtt atgtggaagt tacaggcgat aaagtcaccg ttctgattga aaccgctgaa
    13981 aaatcagatc agattgataa agaacgggcc cttaaggcca aagataatgc tgaaagccgt
    14041 ttatctcaga tgaacaaaga tgacgtggaa tttgaaatta tgcgggcggc actggcgaga
    14101 gccatcgcga gaatcaatgt agcagaaaag caataaaaaa atgcgataag gattttgttg
    14161 cgattgcgat ttaaaatccc agaagaacat gaaagaggct aaagtatatt tagcctcttt
    14221 ttttatgaag agctattgga ttaattttca aaaatacttt caatatgcag tttgtcttcc
    14281 aattgtctgt tggcttcgtc cagtttttcc ggggaggaat aaacggccac tgacgctgat
    14341 ttgaacgcct gagagaaata accggataat acttttttca gtttttgcgg tgtggcggat
    14401 aaaatgtcgt ctctgaattt ctggcgaatc ttatcggtta ttccggcaaa attgcgcatc
    14461 attgccgtat accctttgct ggccggatcc gaaggtttgt caatcatgct gatggtgctg
    14521 atgatcgcct tttccatgtc cggtgaagaa atttcattct gactgtaaaa cgtctgtgcg
    14581 tttttaaaca cctgaagcgt ctcggaaatg cgcgggtcgc gataggacaa aaacgaaaac
    14641 aatcccatgg aagcgtcgaa agaagacatg ccgccgtaag cgcctccctg aacgcggata
    14701 tacttgtaca gatagttatt ggataactct tttgcggcaa tcatcaaaag agggcttgcc
    14761 gggtcggtat aacaaggcgt ttttaaaatg taggcaacat acgatacctg cgcggggaca
    14821 gagattcccg caaataccgg attcaaagaa ggttcttctt ccggagcggc ctgtgaagac
    14881 gccggaagcc tgtcgagtag acacggcagg tgttctttgg caatttccaa cccctgatgc
    14941 tcggcggtga cattaatgac catgttgttt ttattgaaca gggtttgctt taaagtttcc
    15001 agtttatcgc gcaaggcact ttttgatgaa acgaactcac ccgctgttgc ttgaacgaat
    15061 ttaaattgcg ttcgtccgtg ccattgttca tcccggtaag ccgggagggt gagtgccgcg
    15121 ccggctgcca ttctggcgaa tagatgaccg gagggaagaa tcgctgattg aagattgttt
    15181 tttctttctg aaatcagatc ccgcatccgg gcttcgtgac ttaaatcacc gcggcagagc
    15241 acatcgctga tgatgctcat ggcttccgga agattgcgat atagcgcctt aaacgagaaa
    15301 atcatcttct gccatgttga gtcgccattt gcggtaaatc ctgcgcccag attatagccc
    15361 aacccgccca tcttcagagc aatccgtttg gccatttcct cataggtcaa accggcagcg
    15421 cccatgccgg tcatcatctt tcccaaaagc ggcagataga tctgcagctc ttcagggata
    15481 tctgccagat caaaagccag atcaacatag gcaattccgt tggtaaacag atcgtgctcc
    15541 cagaccggaa cgtcaccgat caaagaattt cgggtcggcg ttttttcaat ggatttcgga
    15601 atatcctcca tgttcagcct gggcagggct gaggccgctt ccggcggatc ctgttgattt
    15661 tgaaattcct tgagcttctt ggcttttgca ttaatctgtc ttaattcttc ttcacaaaga
    15721 gatgccttga gcgtgtccat tttttctctg taagcagcct cttttttttc gttgaaatcg
    15781 ggatcgggtt ccataacggc cagaacgcga tggggattat tcaaaagcca tttttcagtc
    15841 attttctgga aaatcagggg atcatcagcc cagcagcggc ggatattctc tatgatttgt
    15901 gaaaaatcca gtccgattaa cggatcgccg tcataaagcc atgtttgaaa aaccgcaccc
    15961 atcagagaaa ttccgtaagg ataagaaccg cgaacaattt ccttgccgtg aaattcgacc
    16021 tgatgcagaa cgccttcgat gagttgcgtg tcgtaaccgg aatcaataat gtcttttaaa
    16081 gtctttagaa tcagttgttc gattttttgt gcgtccgacc gctgtgtgtt tctcaacccg
    16141 acacagaaca ttaattgttt caggtctgct tcgacgccgc tcaccggagt cagatcctct
    16201 cccagaccgg agtcaatcag cgctttgcgc agcggactgg ccgcgctgcc aacgagaaga
    16261 gcggatatga tttgcaggat caatgaggtc tcaaaatcgg tattttcaga catcagccaa
    16321 gcgatgttaa caacggtttt acgttcaaca gattcatctt ttccgatggg ataccctccc
    16381 acaaccgaac gcggctgggt gaaacgcttc tggcttccga tggcggaatt tatttctata
    16441 taattgaaat cctttagtat ttcagaaaga aacaccagat ggtccttcgt gggaatatca
    16501 ccgtaaataa aaaaccttgc attggagggt gagtaatact tgctgtggaa ttcgcaaaat
    16561 tgttcatagg tcagcgaggg gataacgttc ggatccccac cggaatcaaa tgagtaaaca
    16621 ctgtcggggt agacattttc ctgaagcgtc ttatagatca gagaatcggg ggaggaatag
    16681 gcgcctttca tttcattata aactattccg gaaatgatca gatcactgga cagatcatcc
    16741 aaaacagaaa attccagatg atggccttcc tgaagaaaag tctccctgag cattcgcgga
    16801 tgcagaacca gatcggtata aacgcgggcc agattgtaaa aatcggtttt tatctgactg
    16861 gctaccggat aaacggtttt atccggatag gtaaacgcgt tgatgaatgt ttgcatggtt
    16921 gagcgcatta actccttgaa aacgtctttt aagggatatt tttcagagcc ggccagcacc
    16981 gagtgctcca gaatatgcgg caggccggtg gaatcgtacg gaggcgtgcg gaatcctatc
    17041 gcgtataaat tttcgcggtc gaagcagtgc agatgcagta gcttggcgcc ggttttattg
    17101 tgtatcattt cataagctgt cgtatgaatc gtggggaagg tttttatgcg tgtaaccgtg
    17161 aagccgtaaa gctcatctcc ttcacgtaat gtcggttctg gaaaatctga tttctctgac
    17221 attcaattaa actcctcttg aatagatata tggcgaagcc gtcttcaaaa aattagccgc
    17281 cagcatgttc agatggatat gttcaggccg atgaaaagcg agttgacaac cgaccataat
    17341 aaaactgcgg ccgtgagcag gatacaggcg taagccagca ttttgttcca tgcggtttcc
    17401 tgcttattgt cattgtaccc catcagaaac gaaaaaccga tacccgagag cagtccgccg
    17461 gcatgagccc agttgtcgat acctctgaaa atcagaccga aaatgaccag ggcgatgacc
    17521 cagcccatgg cctgcttgta aatggcttct ccgtaggaat cgccgcggct tttcccgtag
    17581 taaatgattg caccgatcag accgcagatc gctgcagaag ctccgatcgt gaatggcgtt
    17641 cctgaaatca ctgaagcgga aaatccacat atgccggtaa cgatatacag attgataaac
    17701 cggtgaaccc cgaattcccg gataataaac ggtcccagct gatacaatgc catcatattg
    17761 aacagaatgt gaaaaattcc cccatgtaga aaagaagccg aaatcagggt ccaaaaacgg
    17821 tgggctccaa taacgggaaa ggtgccgctt gcacccagca gaaaaagact ttgattggac
    17881 ggtgccagta tcccgaaagg accgctgccg ccaaaaatgc cctgcgggtc cagaaatatc
    17941 gaaaaaatat acaaagcaac attaacgtaa atgatgatca tgaccggatt ggacgccata
    18001 aaaagttttc caatattgcc ggcgatattg tgcatcccgg gacgttgaag tccgcaatag
    18061 gggcaggagg gttcatccat gctgatcaat ttacggcaat tgggacagag tattgatttt
    18121 cgttccggca tttttatcct tttttaaaaa aaattgttct gtaaccgttt caggactgtg
    18181 tttaattaac aacattatta tgagttgtaa agaaaaatac catcatttat tgacgattga
    18241 tttattgatg tgttacgtca gacacggagc attgttttga aatgaaaaat cgatctgtga
    18301 taaaaattga atcggtggct tttggcgggt cgggagtcgg tcgtgcggat aaccttgtcg
    18361 tttttgttcc tttttcagcg cccggtgatg aactggaaat cgaaatcacc caacggaaga
    18421 agaactttct gcgcggccga atccggaaca tcatcaagcc gtcgccctgg cggacggatc
    18481 ccgtctgccg ttattatggc gtatgcggcg gctgctgtta tcagcacatc cattatgcgt
    18541 atcaactggc catcaagaaa aaacaggtgg aagatgcttt tcggagaatc ggcaaagttg
    18601 acgcgccgcc tgttttggaa accgtagctt ctccacgaat gtatcattat cgcggcaagg
    18661 cccggtatca tgtaggagac gatccccaaa cagggcacat cggatttctg gatatatccg
    18721 gcggaaaaat tgtcgacatt gagcattgcg caatcatgca ggaaaccatt aatgagcaat
    18781 tgagtatctt gcgggagaat cgacaaaatc tgtggcataa agatgagctg acaatctggt
    18841 cccgatatcc ggatgttacc tctgaagatc agacgtcggt cgtccgtatt gtccagggaa
    18901 aaaggattct ggcgcctgat gacgggtttt tccaggcaaa tgaatatctg acggatcggc
    18961 tggtggatga agtttgtcga ctggtaagac ctggaaaagc agaaaacgca attgacgcct
    19021 tttgcggaag cggccttttc tccatttttc tttcaccttt tgccgaggac attaccggga
    19081 tagaaatgga caaggaatcc gtaaaatacg caaaaataaa tgctgcaaat ctgaatgccc
    19141 gaaatgtccg ttttcttgac gggaatgttg aaattgtatt gcgggaaaaa ttcctgtcct
    19201 tgggcagtaa aattgatctg atcgttttgg atcccccgcg tgtcggttgt gaaatgacgt
    19261 tgttgaaaac tctaatggat ttgcaaccgt cgaacattat ttatatttca tgtaatccgg
    19321 ctacgcaggc gcgggatatc aaatacttgt gtgaaggcgg atatcatttg caaagtcttc
    19381 atccgttgga catgtttccg cagacagaac atattgaagt gatcgggtac ctaaaacgca
    19441 tcatggcatt gtaacttttt tgggggaaat cggcccgtcg atgacaataa tatttataga
    19501 tttaaataaa aaaaattgtt attaaaagcc acctaaataa acaaggagag ggaagatggc
    19561 atcaaaaagc aaagatgtaa gaattgaaca actccggatt ttcgaaaaga aactggctgt
    19621 gcgtttgcag cagcttgagc agaagggaat cagccctgaa aaagcccaga atgacgcaat
    19681 cgttaaaagc ctgaaagcaa aaatcaaaca gaccaaggca agaatcgcaa cattcgataa
    19741 atttgtccaa cagacgcagg cattggccca ggcaaaagcg gccaaacagg ctgctgtcgt
    19801 tgaagaagtc aaggcgtctg ctgaggaagc aaagccgaaa gcgaagaaga aagaggacgg
    19861 tgccgcaaaa gaacccaaaa agcagcctgc tgcagaagat ggcgctgcca agaaggccaa
    19921 aaaacagccc gctgacggac aggaggcggc accgaaaaag gcaaagaagc agccagacga
    19981 aggcgatgac gccgctccga aaaagaaagc aaccaagaaa aaagaagaat agcgttgaaa
    20041 ttgaaatcaa gaaaaaaggg ggaagctgga aaacttcccc ctttttattt
//
LOCUS       DAONQZ010000043        18918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3297_length_18918_cov_13.745109, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000043 DAONQZ010000000
VERSION     DAONQZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3297_length_18918_cov_13.745109"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLR13_07890"
     CDS             <1..213
                     /locus_tag="PLR13_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19296.1"
                     /translation="YVDAKPSGTSIERLRFIARTFPHSVWLNPMPSDEWYYSTTVKAI
                     RQVFTMYELTLDGLEKAVQFLTSSGK"
     gene            452..787
                     /locus_tag="PLR13_07895"
     CDS             452..787
                     /locus_tag="PLR13_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQC19297.1"
                     /translation="MLIEKKRALREINISEEMFNELLNVFVEQTETALKILKKTVTRK
                     NYEEMRKQAHLISGSAGNLRISGIQKLAKEIELEAMEKREISVIKSHIGKLRTDFEKV
                     KEEVSGGQQ"
     gene            774..1145
                     /locus_tag="PLR13_07900"
     CDS             774..1145
                     /locus_tag="PLR13_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQC19298.1"
                     /translation="MDNSKKILVVDDDKVFLKLVENDLTQEGYLVITAQNGKEAISLA
                     KKEKPALLLLDINMPGISGGDVGNILGSDPLTRNIPIIFLTALLTKKEEETRKNMIGH
                     HFFMAKPYDLKALLNEIRKYI"
     gene            1480..6612
                     /locus_tag="PLR13_07905"
     CDS             1480..6612
                     /locus_tag="PLR13_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19299.1"
                     /translation="MKGKNISQPIGLESDFSGPQKLRALMIDDSEDDVLLIIRHMRKG
                     GFAPIYERIETAAAMTQALEEKQWDIIICDYKMPKFSAPSAIALLKQTKLNIPIIVIS
                     GAIGEETAAECMRMGAHDYLMKNNLSRLCPAITRELAEAKIRDRQRVAELQKDAALES
                     LRNSRELYARLVDTMPDVVIRTSLEGTVLFVNDRALRISGYQREEIEGRNMVLFAAPD
                     DHERLMKNAALMLDRKLGPDEYKIVMKDGRIIPFEVNGDVIRDKKGNPFGFVFVCRDV
                     SDRKLAEDKLRESEERYRAIIENIEDGYAELNLKGDFIFVNDALCRIDGYPKEELLKM
                     RYREMMDEETARKVFDVYNKIFKTGGSENNFEYEIFTKERQKKYVDTSVSAIKDSAGQ
                     VIAFRGIVRDRTERKKAEEELRRSEEKYRNILETIREAYFEVDLAGNFTFFNDSVCRM
                     SGFSRDELRGMNYSRLSANDETTRKVYQTFNRVYKTERPSEGYDWPIKRKDGSRISIE
                     ASIMLRKDSSGNIVGFKGVIRDITERKQAEEMLQASEEKYREIFNATSEAIFIHDAET
                     GKIIDVNDSMIRIYGYNSKEEALRCSFADGSGNPEPDTEEKALRLIKKAIQEGPQQFE
                     WLARRKDGTHFWMEMQLMKTDIGGEKLVLAVGRDITERKQAEEAIRENEVKYHSLFDH
                     SMDAILLTRPDGSIIDANPAACRMFGRTKQEILESGRTGLVDTSDPRLEVLLAERART
                     GKFSGRLFHFRKDGTRFTADVSTSVFQDSAGKFKTSMILRDVSDQERMEIALRESEER
                     YRTFINATSAMVYLKDSRFRYILVNQAMEELLGMNLQDIIGKDDFQLMPPETADYFRE
                     NDTRVVQTKSTVVVEERLHDKIYESTKFPVLLENGDVVIGGISRDITERKQAENKLKE
                     TEERYNALFEQSIDLVFVVGFDGQFLDANSAALDLVGYTRDEISSLSIVNLLDDDQIP
                     VALDIIEEIHQYGVQQGLKELRLRHRDGSAVYVETQGATIMSNGEPVAIQGIARDITA
                     RKKAEEELLSLTRRLNDIIEFIPDATLVIDRDGRVIAWNRAIEEMTGITKDAILGKGH
                     YEYAIPFYGRRRPMLIDLLDKRDKKMEALYKYVERGSKIYAESYISGLRDGQGAHLWG
                     VAAPLFDKDGNRFGSIEMIRDVTEIKAKEKSLEESLRGREIAEEATRAKSAFLANMSH
                     EIRTPMNGIIGLVNLALQTGLTEKQKDYLNKIESSAQGLMVIINDILDYSKLEADKMK
                     LEILDFNLEDVMTHISDIMYPNAVQKRLDLLFHIDRDVPLFLKGDPWRLQQVLMNLIG
                     NAVKFTEKGEVVVRVEACARFKRGRKDYIHLNFSVRDTGIGLTKEQMGDLFELFTQAD
                     SSVTRRYGGTGLGLAISRQIVQLMGGTIEATSEAGKGSVFSFMIPLEPGKKKKGGKTS
                     FSGTPFKNKRVLIVDKNPTAQKILADYLEGIGLSVTTAASGSKALKILKEKDNFHLLL
                     VESGTSAQDGIKIVRMIRKNAALAAIPAVMIVSTSFQDDTKTVAEKLGVKYFLKKPFH
                     RSNLLDAMMNIFGQTIKKVSPAVRNPVKAPATEALAGKRILLVEDNFINQQVAREILQ
                     QAGLKVVVAENGKDALGKIRQEQFDLVLMDVQMPEMDGYQATKIIRRNKKFKSLPIIA
                     MTAQAMSGDKEKCLNAGMNDYIAKPISIQNTLSVLSRWLYSK"
     gene            6783..7682
                     /locus_tag="PLR13_07910"
     CDS             6783..7682
                     /locus_tag="PLR13_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC19300.1"
                     /translation="MISQEAKNVMNLMSMMKLPDAGELPIELCRQVLADMMSLSLPPA
                     GCRFEPVNAGGPEALWVKAVSADDTAVILYLHGGGYAMGSPQTHAGFTGLLSELSRLS
                     VLSVDYRLAPENPYPAAIEDSLSAYCWLLRQGVPAESIVVGGDSAGGGLTFATMLKLK
                     EREEALPAAVFAVSPWVDLAVTGESIEINAARDPMITRSGLDYMAGLYAGHADVRLPF
                     ISPLYADLRGLPPVLIHVGTCEMLLSDSLRIAASLENSGVDCTLRVWEDLFHVFHSVV
                     SLPEAIMATEDIAAFVRNRVGGY"
     gene            7713..8642
                     /locus_tag="PLR13_07915"
     CDS             7713..8642
                     /locus_tag="PLR13_07915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQC19301.1"
                     /translation="MKKETLVLKTTDGMQMFVYKWTPDGADAVKGAVQIAHGMAEHAA
                     RYERFGGALTEAGYAVYANDHRGHGRTAGSPDNRGYFADENGWAKVVEDMHLLTGLIK
                     KDYPQKPFFLFGHSMGSFLSRHYAMLYGRELNGLILSGTGGDPGVAGRAGLFIARMDA
                     RLHGKKAKSEIMNKLSFGAFNHAFKPNRTEYDWLSRDHAEVDKYINDPWCGGVFTAGF
                     FCDMLEGLSYINKKDNIAKIPKHLPVYLFSGSKDPVGANTRGVIQVCNTFKREGISDV
                     VLRFYQDGRHEMLNEINREDVFRDVIAWLDQKR"
     gene            8804..10540
                     /locus_tag="PLR13_07920"
     CDS             8804..10540
                     /locus_tag="PLR13_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19302.1"
                     /translation="MATQIPRKRTIFFIRLMVVITTAYFILLSPVAGKELEIYGYIFI
                     AVYLMTNLIVAYIPEKYFHDNRIFYGFILCDSILLPAGIYFSGYIGSDLYLMFFFIIS
                     LTTMSSRFGYLMINTFLFCLIYLWLLHEKGVLTGPKAGAYLIQIPFIIVIAIFYGYLI
                     TTRLKDKDMRINEVRQRYEQVVQATDVLMSIVDRDGKLKFANDIFARFYGYHEDKRLA
                     GLTIAQVNAEDASEADKLLDYVQSVYQKNDMVRYESYNKKHNLWFANTLGPIRDPSTG
                     DVLDVCVISKDITDRIEKEKKLNNTVELLIKTRDQLIQQDKMAALGRMASGIAHEIRN
                     PLEIIYMGIDYLENNIAHQDPQIDGSIEKIYKAANRADNIINNILSFSRKSEYKITQI
                     PVCQLLDNTLVLANQALHKGHVTVKREYETESLEAAGDYNMLEQVFLNLINNAIDAMK
                     ESTEKRLTIRASGKIISEIGYKTGYRYTDFFRIGEKKIVVEISDTGKGMSKDVQSKIF
                     EPFFTTKSVNEGTGLGLSIVHMIIERLSGTIDVTSEENKGTTFYVNIQPQIKKVVAEE
                     GQYDKSDQSSGY"
     gene            10509..10898
                     /locus_tag="PLR13_07925"
     CDS             10509..10898
                     /locus_tag="PLR13_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQC19303.1"
                     /translation="MTNQIKVLAIDDEVDFCYFIKKNLTQSGLFDVVVANNGADGIKL
                     ARKEKPDIILLDLFMPDIPGEDVAAELKDHPATADIPILYITALASNDEIVEGESNKM
                     GDNYIMAKPVRTKKLIETIMKILGQSY"
     gene            complement(11006..>11588)
                     /locus_tag="PLR13_07930"
     CDS             complement(11006..>11588)
                     /locus_tag="PLR13_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19304.1"
                     /translation="FKYRPRKIPDFTMLDDGSMIEIGDYKLQCILTPGHTQGHICLYE
                     EDKQILFSGDHILYDITPHIESWSYEVNSLENYMQSLDKVRNLPVKLVLPGHRNFFTD
                     LKGRIDELKNHHEMRTKEVLDVLGKSTMSAYDIAAGMTWDIDCESWEKFPVAQKWFAV
                     GEAIAHLLYLESKGKVKRHPGEKTDFFSAIRME"
     assembly_gap    11589..11688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11689..12138)
                     /locus_tag="PLR13_07935"
     CDS             complement(<11689..12138)
                     /locus_tag="PLR13_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19305.1"
                     /translation="MYEQILPNLYRIIIPLPNSPLKDLNSYVIKADNRNLIIDTGFNR
                     GVCYEAMENGLHALEIDLSRTDFMLTHMHSDHTGLVSRLFTETSKVYFSRIDSEVFNE
                     NIDWQAMLDYALHNGFELEELIRAAESHPGFKYRPRKIPDFTMLDDGS"
     gene            12449..14536
                     /gene="fusA"
                     /locus_tag="PLR13_07940"
     CDS             12449..14536
                     /gene="fusA"
                     /locus_tag="PLR13_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQC19306.1"
                     /translation="MKIDDQKIRNIGISAHIDSGKTTLTERILYYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKGHEVNIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCAVGGVQSQSITVDRQMKRYRVPCIAFINKCDRSGANPFRVISQLKSKLGHNA
                     VAMQIPIGLENDVQGVVDLVSMKALYFDGDNGETVREAEIPSHLLEEAKAKREELIDA
                     ASMFSDELTEAILEEKEVSADLIYSALRKGTLQREITPVYLGSAYKNKGVQPMLDGVT
                     YLLPCPSDIENVAIDMDKNEETVVLNNDTEKPIVALVFKLEDGQYGQLTYIRVYQGTL
                     AKGSTIVNVRTGKKVKVGRLVRMHADQMEDVESLRAGYIGALFGIECSSGDTFTSPDV
                     NLTMISMYVPKPVISLAIVPKDNKSQVAMAKALNRFAKEDPTFKTYVDHETGDTIIEG
                     MGELHLDIYVERMRREYGADVSTGNPRVAYRETITQRADFNYTHKKQTGGAGQFGRVA
                     GYLEPIADTDFVFENKIFGGAIPSQYIAACEKGFKQSMAKGPKLEFPVTGVKVVINDG
                     ASHAVDSSDMAFQAAARGAFKEAYLRAKPVIHEPIMKVVVETPAEFQGSVMGLLNQRR
                     GMIIGSQDEDVMCVIEAQVPLAEMFGFSTVLRSATQGKAQFTMEFTLYRQVPQSIAEK
                     IALEAAQNKKTAA"
     gene            14550..15320
                     /locus_tag="PLR13_07945"
     CDS             14550..15320
                     /locus_tag="PLR13_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC19307.1"
                     /translation="MQTISVALKREKNIIHLTYKDIVMIKKEMIYRNPLVKLGYDNED
                     ILSTGGFGAVLAYAGVGKTALLVQLALNMMLREHSVLHVSLNDAVSKVDLWYQEIFHD
                     IAEKFGIAEVNEYWDKIQPYRFIMTFKVEGFSAPKLEERLTDLMEQNIFKPHTVIIDG
                     FRFDEAGRGQLVQLKELAGKYSMGLWFTVHTHRHEPPQENGLPLSFLHIADLFEVIVQ
                     LATKGDEVYITSVKGQPKNSAQNDLLLDPATMLIKESQ"
     gene            complement(15466..16413)
                     /locus_tag="PLR13_07950"
     CDS             complement(15466..16413)
                     /locus_tag="PLR13_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC19308.1"
                     /translation="MRKTIIATVMMMLSAFFITTAQADIRPGSYTVTPFIGGFIFEGN
                     EYYRGFDNQKNGRNTLDDTYAVGVRAGYNFTENLGLEGFFSYIPTSRDNFICCDRKKD
                     NDLDLYYYGIEGLYHFMPYGRLVPFVAVGLGGIRYDAPSGEDNGNKFAVDYGAGVKYS
                     VTDKIDLRADVRHVLPLNERYNDLLYSFGVTFSFGGAEKGKKLLDIKFDFDKTDIKAA
                     YHKDINAVTKLMQKYPDLNIVTEGHTDNIGTPEYNKNLSNKRADAVKKYMVEKGGVAA
                     GRIDPKGLGEGSPAVSNDTEAGRAENRRVEAAVDYTGKK"
     gene            complement(16691..17266)
                     /locus_tag="PLR13_07955"
     CDS             complement(16691..17266)
                     /locus_tag="PLR13_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19309.1"
                     /translation="MPILYSPLKDDYQLSPGFTDREQGEKQLDEFFEEDQRLYRNLSR
                     LTTVIDDAEDDPGDEAEIHSYPEAHNGILEGLTATEWFTAGRQAASDRRWDDALNAFN
                     KAIALNPENEEIYFHRGNVYDALGNNKKAVSNFNKAIKLNPLYVDAYLSRAFSYNNLA
                     QANQALADIKKAARLGSLHAQKFLKKSNLAW"
     gene            complement(17534..18403)
                     /locus_tag="PLR13_07960"
     CDS             complement(17534..18403)
                     /locus_tag="PLR13_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQC19310.1"
                     /translation="MDGITALINQILNVFRIPLFHMGEAPFTLWTFIYLVAATLILTL
                     AASALNKVIVTRLLTKSNIERGVRIAIGSIVKYIVLVVGFIIILQTAGINLSSLTILM
                     GALGVGIGFGLQNITNNFVSGMIILLERPIKVGDRIEVANVSGDVVDISMRATTIVTN
                     DNISIIVPNSQFISETVINWSHTDRSVRFNFPIHVSYREDPQAVRKLLLDIALNNPGV
                     LKDPPPDVLFSEFADSAMSFNLRVWTREYINRPGVLKSQLYYQIADRFREKGIEIPFP
                     QQDIHIKETARQS"
     gene            complement(18412..>18918)
                     /locus_tag="PLR13_07965"
     CDS             complement(18412..>18918)
                     /locus_tag="PLR13_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQC19311.1"
                     /translation="AMIGAILLVLSGCITLEEAYDAIDWQVIFLLGGVLSLGIALEKT
                     GAAMYLSNNLIYVLGDLGPVAIVAALYLLTSILTELMSNNATAILLAPIAIAAANGIG
                     VSPTPFLMAIAFAASASFMTPVGYQTNTMIYGVGQYRFSDFIKVGTPLNIMFWILATL
                     FIPVFFPF"
BASE COUNT         5250 a         4203 c         4766 g         4599 t
ORIGIN      
        1 tatgttgacg ccaaaccgtc gggaaccagt attgagcggc tgcgcttcat cgcgagaacc
       61 tttccccaca gcgtatggct gaatcccatg ccttctgacg aatggtatta cagcacgaca
      121 gtcaaggcga tccgacaggt ttttaccatg tacgaactca cgctcgatgg tctggaaaag
      181 gccgtccagt ttttgacatc gtccggaaaa tagaatgcct gcaggtaaaa tatttttgtt
      241 gattaacgga tttttcttta cccgctgttc ggtgaaagat tccatttaat cttccggcaa
      301 ataaaagaaa caaagaaaca ctatacactg cgctaatttt attgtattat acgccataat
      361 ttataataca gcgatagatg catcaacggg aaatcatccc ggtaactccg tcgtctcata
      421 ttggttgaaa aatcttctcc ggaggaattt cttgctgata gaaaagaaaa gagctctacg
      481 ggaaataaac atttccgaag aaatgtttaa tgaattgtta aacgtatttg ttgagcagac
      541 cgaaaccgct ttaaaaatac tgaaaaaaac cgtcacccgt aaaaattatg aggaaatgcg
      601 caagcaagcg cacttgatca gcgggtcggc gggaaacctg cgaataagcg gaattcagaa
      661 gctggccaaa gaaatagaat tggaggcaat ggagaaacgg gaaatttctg ttatcaaaag
      721 ccatattgga aaattgagaa ctgattttga gaaagtcaag gaggaggtgt caggtggaca
      781 acagtaagaa gattcttgtg gtagatgacg acaaagtgtt tctcaaactg gttgaaaatg
      841 atttgacgca agaaggttat ctcgtcatca ccgctcagaa tgggaaagaa gccatatccc
      901 tggctaaaaa agaaaaaccg gctcttttgc tgcttgatat caacatgccg ggcatcagcg
      961 gaggagatgt cggaaatatt ctgggaagcg atcctctgac gaggaatatc cccataattt
     1021 tccttacggc tttattgacc aaaaaagaag aagaaacccg taaaaatatg atcggccatc
     1081 attttttcat ggccaaacct tacgatttga aggctttatt gaacgaaata cgcaagtata
     1141 tatgatcctt tttgccggat cggattcggt tggccttgct ttttgtttaa aacatgacac
     1201 gtaacagaaa tttcataacc gggaatgaat agaaagggta ttcatgtctg acggtttttt
     1261 tcaccggaga cattatggga tatcatacgg taccggacac atcaaatcat ttttattccc
     1321 tttcctgttt tttccatatc ttttttgttc atttcatccg cgagaaaaaa atggccataa
     1381 acgtttcgga acacgccggc aaatcaatga ttcaaatcgg tttgcaaaaa aatcttaccc
     1441 atggacgagg gtaaaccacg tcacaggaga ggataagtca tgaagggaaa aaacatatcg
     1501 caaccaatcg ggctggaatc ggacttcagc ggtccgcaga aactgcgggc attgatgatc
     1561 gatgattcgg aagatgatgt gttgctgatc atacggcata tgagaaaagg cggatttgcc
     1621 ccgatctatg aacgcatcga aaccgccgct gccatgacgc aggccctcga ggaaaagcaa
     1681 tgggacatca tcatttgcga ttataaaatg cccaaattca gtgcgccttc ggccatcgcc
     1741 ctgttaaagc agacaaaact caatatcccc atcatcgtca tatccggagc catcggcgag
     1801 gaaaccgccg ccgagtgcat gcgcatggga gcccatgatt atttgatgaa aaacaacctg
     1861 tcccggcttt gtccggcaat cacccgggaa ctggcggagg caaaaataag agaccggcag
     1921 cgggtggcgg agttgcagaa agatgccgcg cttgaatcgt tgcgcaacag cagggagtta
     1981 tatgcaagac tggttgatac catgccggac gttgttattc gaaccagcct ggaaggcacc
     2041 gtgctgttcg tcaatgaccg cgctctccgg ataagcggtt atcaacggga ggaaattgaa
     2101 ggccggaaca tggttttgtt tgcggctccc gacgaccatg aaagactgat gaaaaacgca
     2161 gcactgatgc ttgatcgcaa gctgggtccc gatgaataca aaatcgttat gaaggacgga
     2221 aggataattc cctttgaggt gaatggcgat gtcattcggg acaaaaaggg caatcccttc
     2281 gggttcgtgt ttgtctgccg ggatgtcagc gaccggaaac ttgctgaaga caagctgaga
     2341 gagagcgagg aacggtaccg ggccatcatc gagaatattg aagacggata cgccgagctt
     2401 aatctgaagg gcgatttcat ctttgtcaat gatgcgctct gccggataga cggatatccg
     2461 aaagaagaat tgttgaaaat gcgctaccgg gaaatgatgg atgaagagac cgcccggaaa
     2521 gtattcgatg tatacaacaa aatcttcaaa acaggtgggt cggaaaataa ttttgaatac
     2581 gaaatattca caaaagagag gcaaaaaaag tatgtagaca cttccgtatc ggccattaaa
     2641 gattcagcag gtcaggttat tgccttccgc ggaatcgtgc gggatagaac cgagcgcaag
     2701 aaagcggagg aagagcttcg ccggagcgag gaaaaatacc gcaatattct ggaaaccatt
     2761 cgcgaagcct attttgaagt ggacctggcc ggcaatttta ccttcttcaa cgattcggta
     2821 tgccggatgt cggggttttc ccgggacgaa ttgaggggga tgaattacag ccgcctttcg
     2881 gcaaatgacg aaaccacccg gaaagtttat caaacgttca accgagtata taaaacagaa
     2941 cggccgtcag aaggctatga ctggccgata aaaagaaaag acggctcccg gatatccata
     3001 gaagcttcga ttatgctgcg caaggattca tccggcaata tcgtcggttt caaaggtgtg
     3061 atccgggata ttaccgaacg caaacaggcc gaggaaatgc tgcaggcgag cgaggaaaaa
     3121 taccgggaaa ttttcaacgc cacatcggaa gccattttta ttcacgatgc agaaaccgga
     3181 aaaattatcg atgtcaatga ttcaatgatc agaatttacg gctacaattc aaaggaagaa
     3241 gcactcaggt gtagtttcgc tgatggaagc ggcaatccgg aaccggatac ggaagaaaaa
     3301 gcgctgagac ttatcaaaaa agcgattcag gaagggccgc aacaatttga atggctggcc
     3361 cgcaggaaag acgggacaca tttctggatg gaaatgcagt tgatgaaaac cgatatcggc
     3421 ggcgaaaaac tggttcttgc cgtcgggagg gatatcaccg aacgcaaaca ggccgaggaa
     3481 gccatccggg aaaatgaagt gaaatatcac tcgctgtttg atcactcgat ggacgccatt
     3541 ttgctgaccc gaccggatgg ttcgattatt gatgccaacc cggcggcctg caggatgttc
     3601 ggccggacaa agcaggagat cctggaatcc ggacgtacag gactggtgga tacatccgat
     3661 ccccgtctag aggttttgct ggcggagaga gcccgaaccg gtaaattcag cggccggctg
     3721 tttcatttcc ggaaagacgg aacacgcttt acggcggatg tgagcacatc cgtttttcag
     3781 gacagcgcag ggaaattcaa aacaagcatg attctgcgcg acgttagcga tcaggagcgc
     3841 atggaaattg cccttcggga aagcgaagaa cgctatcgca catttatcaa tgccacttct
     3901 gccatggttt atcttaaaga cagccgattc cgctacatcc tggtaaacca ggccatggaa
     3961 gaacttctgg gtatgaatct gcaagacatc atcggaaagg acgatttcca gctgatgccg
     4021 ccggaaacgg ccgactactt tcgggaaaac gacaccaggg tcgtgcaaac aaaatcgacg
     4081 gttgtcgtag aagaaaggct gcacgataaa atatacgaat caaccaaatt tcccgttctg
     4141 ctggaaaacg gcgatgtcgt tatcggcggc atttcccgcg acatcaccga gcgcaaacag
     4201 gcggagaata aattaaaaga aactgaggag agatataacg ccctttttga gcaatcgatc
     4261 gatcttgttt tcgtcgtcgg tttcgacggg cagttccttg atgcaaattc cgccgccctt
     4321 gatctagtcg gttatacaag agatgaaata tcgtcgttgt ccatcgtgaa ccttctggat
     4381 gacgatcaga tccccgtggc gctggacatt atcgaagaaa tccatcaata cggggtacag
     4441 cagggtctaa aggaacttcg gttgcgtcat agagacggaa gcgctgttta tgtggaaact
     4501 cagggagcaa ccatcatgtc caacggagaa ccggtggcca ttcagggaat cgcccgggac
     4561 attaccgccc gcaagaaagc ggaagaagag ttgctttcgt tgacccggcg gttgaacgat
     4621 atcatcgaat tcattcctga cgcaacattg gtcattgacc gcgacggcag agtgattgcc
     4681 tggaaccgcg ccatcgaaga aatgacgggt attacaaagg acgccatact gggcaagggg
     4741 cattatgaat atgcgatacc cttttacggc cggcggcggc ccatgctgat cgatcttctg
     4801 gataaaagag acaaaaaaat ggaagcgtta tacaaatatg tggaacgcgg cagcaagatt
     4861 tacgccgaat catatatttc cggactgcgt gacggccagg gagcgcatct gtggggtgtg
     4921 gcggctccgc ttttcgacaa ggacggaaac cgcttcggtt ccatcgagat gatccgggat
     4981 gtcaccgaga tcaaagccaa ggaaaaatcg cttgaggaaa gcctcagagg cagggaaatt
     5041 gccgaggaag cgacaagagc caaaagcgct ttcctggcca acatgagcca tgaaatccgg
     5101 acaccgatga acggcatcat cggtctggtg aatcttgccc ttcaaaccgg cctgacggaa
     5161 aaacagaaag actatctgaa taaaatagaa tcttcggcac agggactgat ggtgatcatc
     5221 aatgatattc tcgattattc caaactcgaa gcggacaaaa tgaaactgga aattctcgat
     5281 ttcaatcttg aagatgtcat gacgcatatt tccgatatca tgtatccgaa tgccgtgcaa
     5341 aaaaggcttg atctgctctt ccacatcgac agggatgtcc ctctcttttt gaagggtgat
     5401 ccctggcgtt tgcaacaggt gctgatgaat ctgatcggca atgccgtcaa gtttacggag
     5461 aaaggcgaag tggttgtccg cgtggaagcg tgcgccagat tcaaaagagg acgaaaggat
     5521 tatatccatt tgaatttttc cgtcagagat acggggatcg gtctgacaaa agaacaaatg
     5581 ggcgatttgt tcgaactgtt cacgcaggcg gacagttccg tcacgagacg ctacggcggg
     5641 accggtctcg gattggccat ttccagacag atcgttcagc tcatgggagg aaccatagag
     5701 gcgacaagcg aagcgggaaa aggcagtgtt ttttctttca tgattcccct ggagccggga
     5761 aaaaagaaaa aaggaggcaa aacgagcttt tccggcacac cctttaaaaa taaaagggtg
     5821 ctgatcgttg ataaaaatcc gacggctcaa aaaattctcg cggattatct ggaaggtatc
     5881 ggcttgagtg ttaccaccgc cgcgtcggga tcgaaagcct tgaaaattct gaaagagaaa
     5941 gacaactttc atctcctgtt ggtcgaatca ggaacgtcgg cccaggacgg cataaaaatc
     6001 gtcaggatga taagaaaaaa tgccgcgttg gccgcgattc ctgcggtgat gatcgtttca
     6061 acatcctttc aggatgatac caaaaccgtt gctgaaaaac tgggcgtgaa atatttcctc
     6121 aaaaaaccat ttcaccggtc gaatcttttg gatgcaatga tgaacatctt cggccaaacg
     6181 atcaaaaaag tctcccccgc ggtccgtaat ccggtcaagg ctcctgcgac ggaggcgctt
     6241 gccggcaagc ggatattgct cgtggaggac aacttcatca atcagcaggt tgcccgtgaa
     6301 attcttcagc aggcggggct gaaagtagtc gttgccgaaa acggaaagga cgccctgggt
     6361 aaaatcagac aagagcaatt tgacctggtg cttatggatg tccagatgcc cgaaatggat
     6421 ggataccagg cgacgaaaat aatccggcgg aataagaaat tcaaatcgct gcccatcatt
     6481 gccatgacgg ctcaggccat gagtggcgac aaggaaaaat gcctgaacgc tggaatgaac
     6541 gattatatcg ccaaaccgat cagtatccaa aatactttaa gcgttttaag ccgctggctg
     6601 tattccaaat agagattcat ggtatcggaa acgatggctg acggagggga aaaggttctg
     6661 tccatcggtt gagattggcg gataacaaac cacagataaa ccatagcgta tgacattgaa
     6721 aaaggcgata cgtctgttat attttttttc atgatacgga catcggtccg ggaggatgtg
     6781 ccatgatcag tcaggaagca aaaaacgtca tgaatctgat gtcgatgatg aaactgcccg
     6841 acgccggcga gttgccgata gagctctgtc ggcaggttct ggcggacatg atgtccttgt
     6901 ctttgccgcc ggccggatgc agattcgaac cggtcaatgc cggaggaccg gaggcattat
     6961 gggtaaaggc tgtatcggcg gacgatacgg cggttatttt atatttgcac ggaggcggtt
     7021 atgccatggg ctctccgcag acccacgcgg gattcacggg gctgttgagc gagttatccc
     7081 gcctgagcgt attatccgtt gactaccgtc tcgctccgga aaatccctat cctgccgcga
     7141 ttgaagattc gctctcggca tattgctggc ttttgcgaca gggtgtgcct gcggaatcga
     7201 tagttgtcgg cggggattcc gccggagggg gactgacgtt tgcgacgatg ctgaagttga
     7261 aagaacggga agaagctctc ccggccgcgg tatttgccgt ttcgccgtgg gtggatctgg
     7321 ccgtgaccgg cgagtccatt gaaataaatg ccgccaggga tcccatgatc acgcgatccg
     7381 gtcttgatta catggcgggc ctgtatgcgg gacatgccga tgtgcgcttg ccgtttattt
     7441 ctcctctata cgcggatctg agagggctgc cgccggtgct tattcatgta ggaacctgcg
     7501 aaatgctcct gagtgactcg ctccgtattg ccgcttccct cgaaaattca ggcgtggact
     7561 gcacgctccg cgtatgggag gatctgtttc atgtttttca ttcggtggtc agtcttcccg
     7621 aggcaataat ggccacggag gacattgccg cttttgtcag gaatcgtgtg ggcggatatt
     7681 aaccttgaat aatgatgtgc aggagaggaa aaatgaaaaa agagaccctt gttttgaaaa
     7741 caaccgacgg gatgcagatg ttcgtttaca agtggacgcc tgacggcgcg gatgccgtga
     7801 agggggctgt gcagatagca cacggcatgg ctgagcatgc cgcacgttat gaacgttttg
     7861 gcggcgccct gaccgaagcg ggatatgccg tttacgccaa tgatcaccgg gggcacggca
     7921 gaaccgccgg atcgccggat aatagaggct attttgccga cgaaaacggc tgggcaaaag
     7981 ttgtagagga catgcacctg ctgaccggcc ttattaaaaa ggactatccg caaaagccgt
     8041 tctttctctt cggtcacagc atgggatctt ttctttcccg gcattatgcc atgctttacg
     8101 gacgggaact caacggcctt attctgtcgg gaacgggcgg tgatcccggc gtggcaggtc
     8161 gggcggggct tttcattgcc aggatggatg ccaggcttca cggcaaaaaa gcgaaaagtg
     8221 aaattatgaa caaattgtcg tttggcgctt tcaatcatgc ttttaaaccc aaccgcacgg
     8281 aatacgactg gctttcccgg gaccatgccg aggtggacaa atacattaac gatccatggt
     8341 gcggcggagt ttttacggcc ggattttttt gcgatatgct ggaaggcctt tcctatatca
     8401 acaaaaagga taatatcgca aaaattccga agcatctgcc ggtctatctt ttttccggtt
     8461 cgaaagatcc cgtgggagcg aacaccagag gcgtgatcca ggtatgcaac acgtttaaac
     8521 gcgaaggaat cagcgatgtc gtgttgaggt tttatcagga cggccgtcac gagatgctca
     8581 acgaaataaa tcgtgaagat gtcttccggg atgtcatcgc atggctggat cagaaacggt
     8641 gatgatcaaa gccgccggga ttagccggtg gcgtcaggat gctgatccgt atgaaacatc
     8701 atcgttaaat catagtttta aaatcataat tttcttgatg aaaaaatgtt tgtcctgtat
     8761 tatttttaaa catcaagcaa cgcctgaatc ccaagggtag ctcatggcga cacagattcc
     8821 aaggaaaaga acgatatttt ttatccggct gatggttgtg attaccacgg cctattttat
     8881 tctgctgtca ccggtagcgg gcaaagaact ggaaatttac gggtacatct tcatcgccgt
     8941 ttacctgatg accaatctga ttgtcgcgta tatcccggaa aaatattttc acgataacag
     9001 aattttttac ggttttatct tatgcgatag tattcttctt ccggccggaa tttatttttc
     9061 cggatatatc ggatccgact tatatctgat gtttttcttt attatttcat tgaccaccat
     9121 gagctcgcgt ttcgggtatc tgatgatcaa tacattttta ttttgtctca tctatctctg
     9181 gctgctgcat gagaaggggg tgttgaccgg tccgaaagcc ggcgcttatc tgatccagat
     9241 tccattcatc atcgtcatcg caatttttta cggctatctc atcacgacgc gattaaaaga
     9301 caaggacatg cggataaatg aagtccggca gaggtatgaa caggttgttc aagccaccga
     9361 tgtcctgatg agtattgtgg atcgcgacgg aaagttaaaa tttgccaatg atatatttgc
     9421 ccggttttac ggttatcatg aagacaagcg tcttgccgga ctgaccattg cgcaggtgaa
     9481 tgccgaggat gcatccgaag cggataaact tctggattac gtgcagtcgg tgtatcaaaa
     9541 aaacgacatg gtgcgatacg aatcctataa taagaaacat aatttgtggt ttgccaatac
     9601 gctcggtccg atcagggatc cgtcaaccgg tgatgttctg gatgtctgcg ttatctcgaa
     9661 agatatcacc gaccggattg aaaaagaaaa aaaattgaat aatacggttg agctgttgat
     9721 caaaacgcgg gaccagctga tccaacagga taaaatggcc gctctggggc gcatggcgtc
     9781 aggcattgcc catgaaatca ggaatcctct tgaaattatt tacatgggga tcgactatct
     9841 ggagaacaat attgcccacc aagaccctca gatcgacggc tccatcgaaa aaatatataa
     9901 agcggcaaac cgcgccgaca atatcataaa caacattctg tctttttcca gaaaatcgga
     9961 atacaaaatt acgcagattc cggtctgcca attgctcgac aacacgctgg tgctggccaa
    10021 tcaggccctt cacaagggac atgtcactgt gaagcgcgag tatgaaaccg aatcgctgga
    10081 ggcggcgggc gattataaca tgctggagca ggtttttctg aatcttatca acaacgccat
    10141 tgacgcgatg aaggagagta cagaaaagag attgaccatt cgcgcctccg ggaaaatcat
    10201 atcggaaatc ggttacaaaa ccggttatcg ctatacggat tttttcagaa tcggcgagaa
    10261 aaagattgtt gtagagatat cggacaccgg aaagggaatg tccaaagatg ttcagtcgaa
    10321 aatattcgaa ccgtttttta ccacgaaatc cgtcaatgaa ggaaccggtc tggggcttag
    10381 tatcgttcac atgatcatcg agcgtttgag tggaacgatt gatgttacca gtgaggagaa
    10441 taagggaacg acattttatg tcaatattca gccgcagata aaaaaggtag ttgccgagga
    10501 gggacaatat gacaaatcag atcaaagttc tggctattga tgatgaagtc gatttttgtt
    10561 atttcatcaa aaaaaatctt acgcagtccg gcctgttcga tgtggttgtg gcaaacaacg
    10621 gcgcagatgg aataaaactt gccagaaaag aaaagccgga catcattctg ctggatttgt
    10681 ttatgccaga cattccgggt gaagatgttg ccgctgaatt gaaagaccat cctgccacgg
    10741 ccgatatccc gattctctat attacggctc ttgcgtccaa tgacgaaatt gttgaaggcg
    10801 aaagcaataa aatgggagat aattacatca tggcgaaacc cgtcaggacc aaaaagctga
    10861 tcgaaaccat tatgaaaata ctggggcaat cgtattagca gccgaaaaca ttcagaatgc
    10921 agaaaaaatc aacagaaaaa accacaggaa gggcggtttg ctctgagcgg cctgcgccgg
    10981 aaaatacaat aagccggaca aaaaatcatt ccatccggat tgctgaaaaa aaatctgttt
    11041 tttcacccgg atgtcttttt actttgcctt tgctttccag gtagagcagg tgcgcaatgg
    11101 cttccccgac ggcaaaccat ttctgggcca ccggaaactt ttcccagctt tcgcaatcga
    11161 tatcccaggt catgcctgca gcaatatcat aggcactcat ggtactttta cccaaaacgt
    11221 caagtacttc tttagtcctc atttcgtgat ggttcttcaa ttcgtctatt cgtcccttga
    11281 gatcggtaaa gaagttgcgg tgtccgggca gaacaagttt gaccggaaga tttctcacct
    11341 tgtcgaggct ctgcatatag ttttccagag aattcacttc gtatgaccaa gactcgatgt
    11401 gcggagtaat atcgtaaaga atatgatctc cggaaaatag aatttgcttg tcttcttcgt
    11461 agaggcagat atgaccttgg gtatggccgg gtgtaagaat gcattggagt ttataatcgc
    11521 cgatttcaat catgctgccg tcgtccagca tggtgaagtc gggaattttc ctcggtctgt
    11581 atttaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tgccgtcgtc
    11701 cagcatggtg aagtcgggaa ttttcctcgg tctgtattta aatcccggat gactctctgc
    11761 ggcccggatg agttcctcca gttcgaaacc gttatgcaat gcgtaatcca gcatggcctg
    11821 ccagtcgata ttttcgttga atacttcgga atcgatcctg ctgaaataga ctttgctggt
    11881 ctccgtaaac agccgggaca ccagtcccgt gtggtcggag tgcatatggg tgagcatgaa
    11941 atccgttctg gataaatcaa tctccaaggc gtgcaggcca ttttccatgg cctcataaca
    12001 gacaccgcgg ttgaatccgg tatcgataat cagattccga ttatcggctt taatgacgta
    12061 agaattcaga tctttaagcg ggctgttggg caggggtatg ataatccgat aaaggtttgg
    12121 caatatctgc tcgtacatca aaaactcctt tataattcaa ttaattaaac atgaaagata
    12181 attatcatgt tcaacaataa tacacaagca ttcttattgt tctaaatatg ttaaataact
    12241 gcttgacata ttgcgttgca atatgtatga agccaagttc acaaatgctt gtttatggtg
    12301 gatgccccat cttgcagaga tacttacaag tgtggggttt ttttgtgaga ctcacgtttc
    12361 agaaatgaaa tgcactgaaa cgtgtaagtc gaacaatccc tgagcgaagc gaatggaatt
    12421 ataacagtga taaagaagga aaaaactaat gaaaattgac gatcagaaaa ttcgtaatat
    12481 cggcatcagc gcccatatcg attcgggcaa gacgacgctg accgagagaa tcctttatta
    12541 cacgaaaaga atccatgcca ttcacgatgt caaaggcaaa gacggcgtcg gcgccacaat
    12601 ggattccatg gaccttgaaa aggagcgcgg cattaccatt gcttcggcag caacgtattg
    12661 tgaatggaaa ggccacgaag tcaatatcat cgatacgccg ggtcacgtcg atttcaccat
    12721 cgaggtggag cggtctctgc gcgttctgga cggcgctatt ctggttttgt gcgctgtggg
    12781 aggcgttcag tcgcagtcca ttactgttga tcgtcagatg aaacggtaca gggttccctg
    12841 cattgcgttc attaataaat gcgaccgcag cggcgccaat cccttccgtg tcatcagcca
    12901 gttgaaatcg aagctgggac acaatgccgt ggcgatgcag attcccatcg gcctggaaaa
    12961 tgatgtccag ggcgtcgttg atctggtcag tatgaaagcg ctttatttcg acggcgataa
    13021 cggggaaacg gttcgcgagg cggaaattcc gtctcatctt ctggaagagg ccaaagcgaa
    13081 acgagaagaa ctcattgatg cggcttcgat gttttccgac gaattgacgg aagcgattct
    13141 tgaagaaaaa gaagtcagcg ccgacttgat ctattccgct ttacgcaagg ggacgctgca
    13201 aagagagatc acaccggttt acctgggatc ggcctacaag aacaaaggcg tgcagccgat
    13261 gctcgatggc gttacctatc ttctgccctg tccgtcggac atagaaaacg tagcgataga
    13321 tatggataaa aacgaagaaa cggtcgtcct gaacaatgat acggaaaaac cgattgtcgc
    13381 tctggtcttc aaactggaag acggtcaata cggccagttg acgtatatcc gggtttatca
    13441 gggaacgttg gcaaaagggt cgaccatcgt gaatgtccgc accggcaaaa aggtgaaggt
    13501 aggacgtctg gtgcgcatgc atgctgatca gatggaagat gtcgaatctt taagagcggg
    13561 ttatatcggc gcactattcg gcatcgaatg ttcttccggc gataccttta catcgccgga
    13621 tgtcaattta accatgattt cgatgtatgt tcccaaaccg gtcatttctc tggcgattgt
    13681 tcccaaagac aacaaatcgc aggtggcgat ggccaaggcc ctgaaccgat ttgccaagga
    13741 agatccgaca ttcaaaacgt atgtcgatca tgaaaccggc gacacgatta ttgagggcat
    13801 gggcgaactg cacctggata tttacgtgga aagaatgcgg cgcgaatacg gcgctgatgt
    13861 ttccacgggc aatccccgtg tggcgtaccg tgaaaccatc acgcagcgcg ccgatttcaa
    13921 ttatacgcat aaaaaacaga ccggcggcgc cggccagttc ggacgggtag ccggctatct
    13981 ggaaccgatt gccgatacgg attttgtttt tgaaaataaa atcttcggcg gcgcgattcc
    14041 ttcccaatat attgctgcct gtgaaaaagg gtttaaacag agcatggcca aggggccgaa
    14101 actggaattt ccggtgaccg gcgtgaaggt ggtcatcaat gacggcgctt cccacgcggt
    14161 ggattcttcg gatatggcgt tccaggcggc ggcgcgcggt gctttcaagg aagcatatct
    14221 ccgggcgaaa ccggttattc atgagccgat catgaaagtc gttgtggaaa cgccggcgga
    14281 atttcaggga tcggtcatgg ggcttttgaa tcagcgccgc ggcatgatca tcggttctca
    14341 ggatgaagat gtgatgtgcg tgattgaagc acaggtgcct ttggcggaaa tgtttggatt
    14401 ttcgacggtt ttgcggtcgg caacacaagg gaaagcccaa tttacgatgg aatttactct
    14461 ttaccggcag gttccgcagt cgattgcgga aaaaattgcc ctggaagcgg ctcagaacaa
    14521 aaaaacagcc gcatagtaat cggccgaaca tgcagacaat cagcgttgcc ctgaaaaggg
    14581 aaaaaaatat tattcattta acgtataagg acattgtgat gatcaaaaaa gaaatgattt
    14641 accggaatcc tcttgtcaaa ctcggttatg acaatgagga tattctgtcc acgggcggtt
    14701 ttggggccgt gctggcatat gccggcgtcg gcaaaacggc attgctcgtg caattggcct
    14761 tgaatatgat gctgcgggaa cattcggttc tgcacgtcag cctgaatgat gccgtcagca
    14821 aggtcgacct gtggtatcag gaaatttttc atgacattgc cgagaaattc ggcattgccg
    14881 aggtgaatga atactgggat aaaattcagc cctaccgttt catcatgacg ttcaaggtgg
    14941 aaggattcag cgcgccgaaa ctggaagagc gtctgaccga cctgatggaa cagaatattt
    15001 tcaagccgca caccgtcatt atcgacggat tcagattcga cgaggcgggg cgcggtcagc
    15061 tggtgcagtt gaaggaactg gccggtaaat actccatggg cttatggttt accgtgcata
    15121 cccatcgtca tgaaccgccg caggaaaacg gcctgccgct ttcctttctg catatcgcgg
    15181 atctctttga agtcattgtt cagcttgcga caaaagggga tgaggtgtat atcacgtctg
    15241 tcaaaggcca accgaaaaat tcagcccaaa acgatttact gctcgatccc gcaacaatgc
    15301 tgatcaaaga gagtcagtaa aaaaagactg acagaccgga aaaaaggatg acagcgctcc
    15361 ctttttcgca ggatgtcctc cggagctgcc ttccccctcc agcctaaaat aaaagcccct
    15421 ccaaagattt gaaggggctt tttgataatg gttcaagcca ttgatctact ttttgccggt
    15481 gtagtccacg gccgcttcca cgcgacgatt ttcagcgcgt cctgcctctg tatcattgga
    15541 tacggcgggg gatccttctc ccagcccctt gggatcaatc cgtccggcag caacaccgcc
    15601 tttttcgacc atgtattttt taacggcatc ggcacgttta ttggaaaggt ttttgttgta
    15661 ttcaggcgtg ccgatgttat ccgtatgtcc ctcggtcacg atattgaggt cgggatattt
    15721 ctgcatcaat tttgtcacgg cattaatgtc tttatgataa gccgccttta tatctgtttt
    15781 gtcgaaatcg aatttgatat caagcagctt tttgcctttt tcggctccgc cgaacgagaa
    15841 ggtaacgcca aaactgtaga gaagatcgtt gtatctttca ttcagcggaa gaacatgccg
    15901 gacatcggcg cgcaggtcta ttttatccgt tacagaatat tttactccgg ccccgtaatc
    15961 aacggcaaat ttattgccgt tatcctcacc ggacggagca tcgtagcgaa ttcccccgag
    16021 tccgacggcc acaaaaggca ccaaccggcc gtaaggcatg aagtgataaa gcccctcgat
    16081 cccgtaataa taaagatcga gatcattgtc ttttttccgg tcacagcata taaaattatc
    16141 tcgcgaggtg ggaatgtagc tgaagaaccc ttccaaaccc aaattctccg tgaaattgta
    16201 gcctgcacgc accccgacgg cataggtgtc gtctaaagta tttctcccgt ttttctgatt
    16261 gtcaaatccc cggtagtatt catttccttc gaagatgaat cccccgatga agggtgtaac
    16321 ggtataagaa ccgggacgga tatcggcctg tgctgtggtt ataaaaaaag ccgatagcat
    16381 catcatcacc gttgcgataa tggtttttct cataatgttc tcctttgatt cgaatttatt
    16441 tataaaaata tattgcttga aaaacaaatc acctgttcca atttcacaaa aaattatcgg
    16501 aagcatttcc cttcgtttaa tttttcacag gcgtttagct ttttcggtcg aaagtataat
    16561 tatattcaaa atgtgtgtca agtaaattca ataataacgt gcggggatga atgagccgtg
    16621 ccgttgttat tcaaacgccg tttgcaaaat ccggacaact cgtaaaatca catgtccgca
    16681 tggacaaatc ttaccatgcc aggttgcttt tttttaaaaa tttctgcgca tgcagactgc
    16741 ccagtctggc ggcttttttt atgtcggcaa gcgcctgatt ggcctgagca agattattat
    16801 aggaaaaggc gcggcttaga taggcatcga catacagcgg gttcagttta atggctttat
    16861 taaaattgga gacggctttt ttgttattgc cgagggcatc atacacatta ccgcgatgga
    16921 agtagatttc ctcattttcc ggatttaagg caatggcttt gttgaaagcg tttaaggcgt
    16981 cgtcccatct cctgtccgaa gcagcctgac ggcctgcagt aaaccattct gtggcggtca
    17041 acccttcgag gattccgtta tgcgcttccg gataggagtg aatttctgct tcatcgcccg
    17101 gatcgtcctc tgcatcgtct attacggtcg tcaaccggga aagatttctg taaagtcttt
    17161 gatcctcctc aaaaaattca tccagttgtt tttccccctg ttcacgatcc gtaaatccgg
    17221 gggaaagctg ataatcatct ttcaacggag agtataaaat gggcaatttt tcagaacagc
    17281 cggttgttat cagccataac gttaccatca ataataaaat tatccggaca ggtctcatat
    17341 cgtcaattcc tgtttatcat gtaacacaaa aaagcaaaat ggaaaaacag taaagcatat
    17401 gtttgccccg ttcatcaaga cagggatgcg ttcaaaatcg gggggatgtt caattataaa
    17461 ataacgatcc ggtgcatccg gtgacaatca ggacttgaca ggcaggtatc atttttgggt
    17521 gattgtgccg ccttcaggat tgccgcgctg tttcttttat gtggatatcc tgctgcggga
    17581 agggaatctc gatgcctttt tcccggaagc ggtcggcgat ttgatagtag agctggcttt
    17641 tgagcacgcc ggggcggttg atatactctc ttgtccagac gcgcagattg aaactcatgg
    17701 cgctgtcggc gaattcggaa aaaagaacat ccggcggggg atctttgagc acgccggggt
    17761 tattcaatgc gatgtccaga aggagttttc tgaccgcctg agggtcttcc cggtatgaaa
    17821 catggatcgg aaaattaaag cggacactcc gatccgtatg actccagttg atcacggttt
    17881 cggaaataaa ctgggaatta ggaacgatga tggaaatatt atcattggtg acgatcgtcg
    17941 tcgccctcat ggaaatatcc accacatctc cggaaacatt ggccacttca atgcgatcgc
    18001 cgaccttgat cggacgttcc agcagaataa tcatgccgct gacgaaattg ttggtgatgt
    18061 tctgtaaacc gaaaccgata ccgacgccta aagctcccat cagaatggtg aggctgctga
    18121 gattgatccc ggcggtctgc agaatgatga taaatccgac aaccagtaca atatatttaa
    18181 cgatcgatcc gatggcgata cgaacgcccc gttcgatgtt gctttttgtc aggagacggg
    18241 taacaatgac tttatttaac gcagacgccg ccagcgttaa tatcagcgta gccgcaacga
    18301 gataaataaa cgtccataac gtaaacggag cctcacccat atgaaataaa ggaatacgga
    18361 atacattcaa aatctgattt atcaaagccg tgatgccgtc catgtcacct cctaaaaagg
    18421 aaaaaataca gggatgaaga gcgtcgccag aatccagaac atgatgttaa gcggcgttcc
    18481 cacctttatg aagtcggaaa aacgatactg cccgacaccg tagatcatcg tgttggtctg
    18541 atatcctacc ggcgtcatga aacttgccga ggccgcgaag gcgatcgcca tcagaaaggg
    18601 cgtggggcta accccgatcc cgttggccgc agcaatggca atgggcgcaa gcaatatggc
    18661 tgtggcattg ttcgacatta attccgtcaa aatcgaggtg agcagataaa gcgctgctac
    18721 aatggcgaca ggtcccagat ctcccaaaac ataaatcaaa ttattggaaa gatacatggc
    18781 cgctcctgtt ttctccagcg cgatacccag cgatagaact ccgccgagca gaaaaataac
    18841 ctgccagtcg attgcatcgt aagcttcctc aagggtgatg cagccggaca gaaccagcag
    18901 gatagcgcca atcatcgc
//
LOCUS       DAONQZ010000044        18387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3418_length_18387_cov_16.845080, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000044 DAONQZ010000000
VERSION     DAONQZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3418_length_18387_cov_16.845080"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..411
                     /locus_tag="PLR13_07970"
     CDS             1..411
                     /locus_tag="PLR13_07970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQC19312.1"
                     /translation="MRDKTLKEGEVIVIRYEGPRGRPGMPETLAVTMGLDLAGFTNVA
                     LVTDGRFSGATAGPCIGHVSPEAASGGPIVVIEDGDMINIDIPARRLDVDLKPQEIAR
                     RLAGWKPLHKQITPGYMRRYVRMVGSAAYGAVLE"
     gene            603..1769
                     /locus_tag="PLR13_07975"
     CDS             603..1769
                     /locus_tag="PLR13_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19313.1"
                     /translation="MSFNERTYVATDSLCPPVANQFVFDGEGVLDPYLWRTAVAVASE
                     ANPGARLILKGHLAWSRWIDSGNPPRVREIDGSRWDGHGPEGAPVLLEPLPFRESPIC
                     EVILVHGPVPRVVFRTHHGVMDGRGTLCWAEDVFRALRKENVMGSTSTLTEVQMAESF
                     QKQFRRAFPVEHIAPTGLPEGDARGVIWKRRSIKGKVPLLLPRCARFAAEEAWRHAEG
                     IVRFAVAVDMRPRRPELRSTGNLTLDLYVEINKNTTPELIADDMAYQLRHGFEGRIKK
                     YDQLVKYIPLRLVRFGAQKMIRDRHRKGRYGLSGIFSNLGRVDLNFFCGGGFSARSFW
                     AVPPATEYFPFFLVLSGYEGGSELVLSVPKNLGSAGRLDDTLECIARKLEQVTR"
     gene            complement(1766..2539)
                     /locus_tag="PLR13_07980"
     CDS             complement(1766..2539)
                     /locus_tag="PLR13_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQC19314.1"
                     /translation="MLPDTASAVNFYDGARAKQGTYFLTYTSVYAADKTTNGKGNHAS
                     KDMGLLNAQELLRLCYYSPDFVATALVPFGLMKIDSLDQESSGIGDINLGAGYFLPIR
                     NVDILPMIFVEYPTGQYSSAKSANIGSNQLDIKPVVFLHKSMGDFSIDAAVKYVFRFE
                     NERTDVLPGDEFHLQCLLGYHLTEKFKLGPSVNWLQSKEKKQNGTAVDDSARAVLSVG
                     ADIYYRFSRFGITLTYLCDVYTENSAQGHFVQIKTVYRF"
     gene            2817..>4591
                     /locus_tag="PLR13_07985"
     CDS             2817..>4591
                     /locus_tag="PLR13_07985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19315.1"
                     /translation="MWKDKVVSPSEVLSQIEPGMTIFLGTGMAEPRTLVKNLMASEEP
                     NLQDLELIQLVSIGDTIPIDERYSRKFRLRTFFSGWIASEAVSAGRIDLIPSRFSKIP
                     QLFKSGAIHIDATFIQISPPDENGYACLVGVDVERQAMETAHLVVGEINAKAPRIMGD
                     TLVHMDEFNFFVESAEPPLYIPRWPVEDVFLKIAANIAAVVEDGSCVSFGIGPLYEAL
                     AVQLTTKKNLGVHSPFFTDSLMDLVQSGAVTNRYKGVFRGKCAASYLLGSEKLMRWLD
                     RNPLVEFQPEDVITDPKIIGTNDKTISILPARKIDLTGNVALHTGKGNVTAGPGNVQE
                     LFIGASLSKNGRTIFGLPSRNRKGLPNIVLSVDNLPFQFTNRESMDLVATEYGVAYLM
                     GKTMRERAQELIDIAHPDDREELVRQAKEAKLLYADQIYFPESGHLYPARFICSHEFK
                     GGLKVLFRPIKPSDEEKMRDLFYRFSDQAVYSRYFTSIKTMPHKKMQEYVNVNYRWVM
                     SIVGVVEAAGTEKIIAEARYARTKQDAFADVAFIVDEDYQNMGIASYLFELLIRAARE
                     EGIKGFTADVLASNKSMLKVFEKAP"
     assembly_gap    4592..4691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4843..5988)
                     /locus_tag="PLR13_07990"
     CDS             complement(4843..5988)
                     /locus_tag="PLR13_07990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQC19316.1"
                     /translation="MISKRASKFTSFIVMDVMAKAEELERQGEKVIHMEVGEPDFPTP
                     PVIMKAARDALAKNKTLYTHALGLLELRQAICDFYYLEYGVSITPDQVLVSTGTSPAL
                     LFAMLAILDPCDEVIISNPRYPCYQNFILAAGGKTREVRTYPEDGFQYRPKDIKKKLS
                     SRTKAIMINSPSNPTGIVMTEEQLQNVAQFDKQYIISDEIYHGLVYKDRAHSILEFTD
                     KAFVINGFSKLYAMTGWRLGYCIFPKTFSKVMQRIHQNFMISANGFIQWAGIAALTKA
                     QKDVARMVRIYDERRRYMLSRLQDMGFVIHVEPTGAFYVFADARRFCKDSYKEAFRIT
                     EEVKVGVTPGIDFGSGGEGFLRFSYANSLENIQEGLNRLEGYLKKRR"
     gene            6306..7109
                     /locus_tag="PLR13_07995"
     CDS             6306..7109
                     /locus_tag="PLR13_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC19317.1"
                     /translation="MRTVLIIILIAVISLGGCNMQNRFLYFPDATWPTKQALANENMN
                     LWQATGDDYQGLVSAIDAPAPSGTIVLFHGNGGTALDRGFYLPPFMNLGFRVILAEYP
                     KYGGRPGKVGEKPFVAAGLETVRLAREHYGDPLYLGGESLGCGVVAAIVRQTPVPVAG
                     IMLITPWDTLASVAKSLFPFFPVKLVLTDQYDSMENLKTFKNKIAVVGAERDEILPVK
                     HAINLYNALPEGRKRLWIIKGAGHNNWPMFTDVSLFREIVDFVKIDVHQ"
     gene            7164..8165
                     /locus_tag="PLR13_08000"
     CDS             7164..8165
                     /locus_tag="PLR13_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC19318.1"
                     /translation="MKQEKNLKIGRRSFLKLTAGAAALSGMSLPKKSFAGTEQRLSTL
                     IDLNLCDGCVDREIPACVGACKEINRDKIPQVIDPIPEPWPRKTIEDWSHKRDVADRL
                     TPYNFIYVHKADVRVAGQAKTIFVPRRCMHCDNPACATICPFSANHKHKSGAVVIDQN
                     LCFGGAKCRDVCPWEIPQRQSGVGIYLNVLPGLMGNGVMYKCDLCVDRLTQGKLPGCI
                     EACPREAMLIGSRAAIEKAALSRAARINGYLYGKTQNGGTATLYVSPVPFEEINKTMI
                     KKPGQPDMKMNVERRMAGTDALGKAVLAAPVLGLAAAAVVGVWGRIISRKEKAGREE"
     gene            8170..8973
                     /locus_tag="PLR13_08005"
     CDS             8170..8973
                     /locus_tag="PLR13_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQC19319.1"
                     /translation="MTDKILTQENRVVRHGAMELIEHWAIALSGLILLLTGIFELPVG
                     KRYYIIDVPGLGWSADFITSLYLHYAAALVFTAAAVFHVFYHGLRGEKGLLPRKGDLS
                     ESVTVIKSLFGFGEEPPMRKYLPEQRLAYAAMALIILMLIISGMVKTYKNIYAPDMSH
                     GILLWATWIHNIGFILFFLAFLAHMAAIALRPNWPMVRGIFTGRVRLDYARHRHPLWI
                     AGMEPEPQDPATVEEGEQQPLPSEEAPVMEETEEEDLPPGKPQPEEDKT"
     gene            9019..10101
                     /locus_tag="PLR13_08010"
     CDS             9019..10101
                     /locus_tag="PLR13_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQC19320.1"
                     /translation="MENTPKTGYKRHTVIEEEIPIYRRISLFRVLHISALMIFILLAL
                     SLVAVSVGSVSIEPGRVLTFFFGPLTGQSPLSSEEELIICSVRLPRIVFAGIVGAALS
                     LGGVVFQAILRNPLADPYILGISGGAALGAIIGIVIGAGAFYLGIPLLAFAGALATVF
                     IVFMMAGKTRGPLLDNSLLLSGVVVNAFFSAAILFFLSIVNSMELHSITFWLMGDLSH
                     ASAREILVAALCLLSGFVLLYAQARKLNLIVQGEETAEHLGVHVARTKKWLMVITSLM
                     IAVAVSLAGIIGFVGMMVPHLMRLVFGSDHRLILPVCALFGASFLIAADTVARVLLAP
                     AELPVGVVTALCGAPYFIFLLKRKRL"
     gene            10104..10883
                     /locus_tag="PLR13_08015"
     CDS             10104..10883
                     /locus_tag="PLR13_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19321.1"
                     /translation="MSVITAENIRFSYPGKPVMNGVSFSIEAAEMVAVIGPNGSGKTT
                     LLKMINGTLFPDGGRMLIDGRETTGWPRKEMARQVAIVPQETAVTFPFYAEEIVLMGR
                     FPHLNNYRFEDKKDYRIVREAMEKTDTISFAARRLDELSAGERQRVLIARALAQEPKI
                     LLLDESTVFLDLKHQAEFLSLLRQLNAVQNLTVMFVTHDINLAAQHAHKIILLDCGKI
                     YAIGMPAEVITAANIREVYDVDVFVDRNPHDGSPRATLLTN"
     regulatory      10932..11132
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11161..13233
                     /locus_tag="PLR13_08020"
     CDS             11161..13233
                     /locus_tag="PLR13_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQC19322.1"
                     /translation="MKKILFFVLFFSVVVFSPANASQKDADNALVTMGEIVVTATRDA
                     QEIRKTPSSVSVITEEEINDSGATTLVEVLDRIESIAFRTYSGNSSQSQIDMRGFGGD
                     NPFGKTLILLDGRRLNRTDMASINWLQMPVNGVEKIEIVRGPGSVLYGDAAVGGVINI
                     ITKKGKGKPTVNLSAIGGSYGLHDERIGVSGATGKWTYALTGENNFSSGYRDRSKYSA
                     RGGGLDVGYFAGDILNMSLGVSFNRVDYQMPGALTKAQMVKDRRQYQPAMPDYYMNAN
                     DDNDGSDKYTNIKFDVKSFWGAWGRTEVNFLYGKKDLQMNMPSWFSYNYSDTDAETFG
                     MTPRYILEKNIFGFHNKVIMGLDYYHEPYAKDIFSNRRRTDRLSTADFTRDALGYYIR
                     DEFSVLKNLILSAGYRFDRVSVEGSNEDFSTPSNNFTNQKNIYKAEAYEAGLTWLWGD
                     QSKIFAKYATVYRIPFLDEVASFSGFGGNFLKSLEKEKGKSMEMGAEYYLLNNLKLGV
                     ALFRLDMEDEIEYVYDPETFTGENRNTGKTRHDGVEASFAYLLQKYFRIFGNYTYHKA
                     TFEKGLYNKKEMPMVPRHAANVGLEIYLPGHVTLRTEVQHVGKAYLSGDSDNSTEKLE
                     DRTLLNIVLQYKPVWDTMKLTAFLGVDNVADVKYSSFGIDYEQYAMPNFYYPMPGVTF
                     KGGVSVEF"
     gene            13223..14140
                     /locus_tag="PLR13_08025"
     CDS             13223..14140
                     /locus_tag="PLR13_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HQC19323.1"
                     /translation="MSFKHLSFKDNAVIVFSVLILAVFCAGLACARTVTDEMGRIVHV
                     RRVPQRIVSLAPGITETLYALGLEDRIVGVTTFCDWPPKARLKGKVGGFTNPSIEKIV
                     SLRPDLIIATADGNKKDTVEKLERLGLPVYVTKPSDTRGVLESIMRIGEITGRTKEAD
                     ELADGLRKRMNRITARIRGKNRPRVFFQLGMDPMITAGRNTLIHEVIEKAGGINIAGH
                     DTANYPRYSTEGIMAASPDIIVFAPMVNDQEFSIVKKFWGRFGKIPAVQNNRIYLIDA
                     DLINRASPRIFDAVERIALIFHPDIKCFQ"
     gene            14260..14796
                     /locus_tag="PLR13_08030"
     CDS             14260..14796
                     /locus_tag="PLR13_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HQC19324.1"
                     /translation="MIDGKLPTRGLVYAALFGAMTAAGALIVIPLPPVPVTAQTFFMN
                     VAALLLGGPLAVVSQIIYVLLGVVGMPVFAGGKAGLGILFGPTGGYLFGFIIAAFVIG
                     HVHRMKKNAGAVWQVFSMVLGMLVIYVAGSIQLAWIANMSLPQVLIVGVVPFIPGDLI
                     KIALAVLISSRLKNRIEI"
     gene            14807..15640
                     /locus_tag="PLR13_08035"
     CDS             14807..15640
                     /locus_tag="PLR13_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC19325.1"
                     /translation="MIVLEDVYCRYEPEGIEALRGVSLQIREGERLALIGPNGCGKTT
                     LIRHCNALLVPEKGHVTVGGLRTDHKENQPDIRSRVGMVFQNPDHQIVGMTVEDDVAF
                     GPENLRLPAAEIKARVAESLTRVGIYGLAARNIEDLSGGEKRLVAIAGVLAMRPRYIA
                     LDEPTAFLDPAASGRVLAIIDKLHAEGVGIIHITHNMAEAARAQKIVVMDQGQICMQG
                     TPEAIFSRFEILQKMGLQLPQITELLIRLKQTGFPVRTGIVHMEDALKEIISMTARRK
                     D"
     gene            15643..16440
                     /locus_tag="PLR13_08040"
     CDS             15643..16440
                     /locus_tag="PLR13_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQC19326.1"
                     /translation="MYRTGSFIRGESFIYRLDPRLKLAVTVLLSLLIFQVEQPLVFAS
                     GIGLFLLAMASGIRLRTIGESIRPVLFFIVLIFLSHLLFSDDKRSMMTIPFLGIAFSS
                     AGLKQGTLVVGQFLCLIVGAVLLTMTTAPSQIIAALRYFLKPFKKLRLPVDDVAVMVA
                     LALRLMPLLLMEKERIDAARKARGFCLRRQSVAGRMNSFLSLISKMLLGIFRKADDIA
                     LAMEARSYRRGGRSSFVELKFSSADYVVLVFLAIFLVIFVALNSHFG"
     gene            16469..17296
                     /locus_tag="PLR13_08045"
     CDS             16469..17296
                     /locus_tag="PLR13_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQC19327.1"
                     /translation="MVDTQNKLKRAVADFERAKILSIDTEYDSFHYFREKLCLIQIHA
                     NHTTYLFDPLGDVALSFLGDFFDNQRIVKILHAADNDIRLLRRDYGFGFRNIFDTHRA
                     AFLLGFRELSLDKMIKEFVGVELKKNKKIQRSHWDNRPLTEEQVEYAVQDVIYLPDLY
                     QKQTAELTAKNLQESAREAFARIAETDWREKKFNRWGYAKIKGYNALNQEQKDLLKKL
                     YLWRFHRAKEENRAVFMFLPDKNLLDLALDADHPEKYLSQRKMRIYGSEIETIIKRM"
     gene            complement(17469..18242)
                     /locus_tag="PLR13_08050"
     CDS             complement(17469..18242)
                     /locus_tag="PLR13_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19328.1"
                     /translation="MTQEKTASLMMLVMFLMIVFIGACATTPATRAVWSKPNAAQQDF
                     AKDMDDCMKKAQATGSASTNVDMYNRCMETRGWTLKEVKSVSESSGITERYTFADGTR
                     YEGTMANGKPHGRGTYNWTNGNKYEGDWFEGKMSGKGKYTCKNGREFTGNFENNKPVG
                     FTINCSGNVYYVPDSRQKAQPRHGSYTDANGNKYEGQIINGKLHGKGKITSPSGNKYE
                     GDFVNGKIEGRGTYTCSTGKTFTGYFKNNKPVGFKVKCK"
BASE COUNT         4324 a         4384 c         4950 g         4629 t
ORIGIN      
        1 atccgtgata aaacgctcaa ggaaggagaa gtcattgtca tccgttacga aggtcccaga
       61 ggccgacccg gcatgcctga gacgcttgcg gtgaccatgg gacttgacct ggccggtttc
      121 acgaatgtgg cgctggtaac ggacggccgc ttttcaggag ccacggcggg accctgcatc
      181 ggacatgttt ctccggaggc ggcgagcggc ggaccgattg tcgttattga agatggcgac
      241 atgatcaaca tcgacatccc cgcgcgccgt cttgacgtgg atttgaaacc gcaggaaatc
      301 gcccggaggc tggccggttg gaagcctctg cataagcaga ttacccccgg ctacatgcgc
      361 cgttatgtcc ggatggtcgg ctcggcggca tatggcgctg ttcttgagtg aatccgcttg
      421 aaagtattcc ggtgggattc attttttcaa tcccgtcagg tttcagacat ttgtttgaaa
      481 cctgattttt ttcttgaggg cagaagccat cataaagtaa ctgaagcacg tatgaagatg
      541 tgataaaatt aatgaaatta tcattgaaag gtgctgctgt ggataagggg tacatgcgaa
      601 aaatgagttt taatgaacgt acgtatgtgg cgacggattc gctctgccct ccggtggcca
      661 accagtttgt ttttgacggg gaaggtgtgc ttgatccgta tctctggcgc accgctgtgg
      721 cggttgcctc tgaagccaat cccggagcga ggctcattct caaaggtcat ctggcctgga
      781 gccgctggat cgattccggc aatccgccgc gtgttcggga aatagacgga agccgatggg
      841 acggtcatgg gccggaagga gcgcctgttt tactggaacc gctgccgttt cgtgagagcc
      901 cgatctgcga ggtgattctt gtacatggtc cggttccccg ggttgttttt cgcacgcatc
      961 acggtgtcat ggacggcaga ggaacgctgt gctgggccga ggacgttttt cgggccctgc
     1021 ggaaagaaaa cgtcatggga tcgacgtcga ccctgacgga agttcagatg gctgaaagtt
     1081 ttcaaaagca gttccgccgc gcttttcccg tcgaacacat tgcgcctacg ggacttcccg
     1141 aaggcgacgc ccggggtgtt atctggaaac gtcgcagcat caaaggcaag gtgccgctgc
     1201 ttttgccgcg atgcgcccgg tttgctgcgg aagaagcctg gcggcatgcg gaaggcatcg
     1261 tgcggtttgc cgtggcggtg gatatgcggc cccgtcggcc ggagttgcgc agcaccggca
     1321 atctgacgtt ggatctttat gtcgagatca acaaaaatac gacgccggaa ctgattgccg
     1381 acgacatggc gtatcaactc cggcacggtt tcgaaggccg gataaaaaaa tatgatcaac
     1441 tggtaaaata tattccgctg cgactggtgc ggttcggggc gcaaaagatg attcgtgacc
     1501 ggcatcgcaa gggacgctac gggctttcgg gcattttttc caatctgggc cgggtcgatc
     1561 tgaatttttt ctgcggaggc ggattttccg cccggtcgtt ctgggctgtc ccgccggcga
     1621 cggagtattt tccgtttttt ctggtactgt cgggatatga aggcggttcg gaactggtgc
     1681 tttcggttcc caaaaacctg ggcagtgcgg gaagacttga tgacactctg gaatgcatcg
     1741 cccggaaatt ggaacaagta acccgctaaa agcgataaac cgtttttatt tgcacaaaat
     1801 gtccctgcgc tgaattttca gtataaacat cgcaaagata agtcaaggtg ataccgaacc
     1861 gggaaaagcg ataataaata tcggccccaa cggaaaggac tgctctggcg ctgtcgtcga
     1921 ctgccgtccc gttttgtttt ttttctttgc tttgcaacca gttgaccgaa gggcccagtt
     1981 tgaacttttc cgtcagatgg taacccagaa ggcactgcag atgaaactcg tcgcccggca
     2041 gaacatcggt tctttcattt tcaaaacgga agacgtattt cacagcggca tctatgctga
     2101 agtcgcccat tgatttgtga agaaaaacaa cgggttttat gtcgagctga ttcgagccga
     2161 tgttggctga ttttgcagag ctgtattggc ctgtgggata ttccacaaat atcatcggga
     2221 gaatatcaac gtttcgaatc ggcagaaaat aaccggcgcc gagattgata tcgccaatcc
     2281 ctgaagattc ctgatccaga gaatcgattt tcattaaacc gaacggaacc agagccgtgg
     2341 ccacaaaatc aggcgaataa tagcaaagac gcaacaattc ctgggcgttc agcagcccca
     2401 tgtctttgct cgcgtgattg cctttgccgt ttgttgtttt atcagcggca taaacggaag
     2461 tgtaggtgag aaaataagtt ccctgttttg cccttgcacc gtcgtaaaaa tttacggcag
     2521 acgccgtatc aggcagcaca agtaacaaga aaaacaacgc gaaaatggat tgtcgccgga
     2581 aacccgataa agccaaaatt gaatcctcga aaccgttttt tttgagaata tgcttataca
     2641 gaacatttcg ccgggggcaa agcataaaaa tattacccgt cggccatcct tccatctctg
     2701 tttaaaaagg tttaattgtc ttgacaggca caggccgcct ccctataatt cagacgttcg
     2761 gtctcgcaac ggcgtaaatt aaattttaac cctacaactg aaaaagggaa taatgtatgt
     2821 ggaaggataa ggttgtttct cccagtgaag tactgtctca aatcgagccg ggcatgacca
     2881 tttttctggg gacgggcatg gcggaaccgc gcacgctggt taaaaatctt atggcgtcgg
     2941 aagagccgaa cctccaggat ctggagttga ttcagctggt cagtatcggc gataccatac
     3001 cgattgatga acgatattcc cgcaaatttc gtctgaggac ttttttctcc ggctggattg
     3061 cgtccgaagc cgtcagcgcg ggaagaatcg atcttatccc cagccggttt tccaaaatcc
     3121 cccagctgtt taagtcgggc gccattcaca ttgatgcgac gttcatccag atatctccgc
     3181 ctgatgaaaa cgggtatgcc tgcctggtcg gcgtggatgt ggagcggcag gcgatggaaa
     3241 ccgcgcatct ggttgtcggc gaaatcaatg ccaaggcgcc ccgcatcatg ggcgacacac
     3301 tggttcatat ggatgagttt aatttttttg tcgaatccgc agaaccgccg ttgtatattc
     3361 cccgctggcc ggtggaagat gtttttctga aaatcgccgc caatattgcc gcggttgttg
     3421 aagacggcag ttgcgtatca ttcgggattg gccctcttta cgaagccctg gcggttcagc
     3481 tcacaacgaa aaaaaatctc ggcgtccatt ctcctttttt tacggattct ctgatggacc
     3541 ttgttcagag cggcgccgtg accaatcgct acaaaggcgt gttccgcggc aagtgtgccg
     3601 cgtcctatct gctgggcagt gaaaaactga tgcgctggct ggaccgcaat ccgctggttg
     3661 aatttcagcc ggaggatgtg attaccgatc cgaaaataat cggcacgaat gacaaaacca
     3721 tatccattct gccggccaga aaaattgacc tgacaggcaa tgtcgcgctg cataccggca
     3781 aggggaatgt caccgccggt ccgggaaatg tccaggaact ttttatcgga gcgtcgcttt
     3841 ccaaaaacgg ccgtaccatt ttcggactgc ccagccgcaa ccggaaaggc ctgcccaaca
     3901 tcgttctgtc cgtcgataat ctgccgttcc agtttaccaa ccgtgagtcg atggacctgg
     3961 tggcgacgga atacggtgtg gcgtatttga tgggcaagac gatgcgcgag cgtgctcagg
     4021 aactgattga tatcgcgcat cccgacgacc gggaggaact ggtccgtcag gcgaaggaag
     4081 cgaaactgct ttacgccgat cagatttatt tccccgagtc cggacatctc tacccggcca
     4141 gattcatctg ttcgcatgaa ttcaagggag gtctcaaggt tcttttccgc ccgatcaaac
     4201 cgtccgatga agaaaagatg cgggacttat tctaccggtt ttccgatcag gccgtttatt
     4261 cccgctactt cacgtccatt aaaacaatgc ctcataaaaa gatgcaggaa tacgtcaacg
     4321 tcaattaccg ctgggtcatg tccatcgtcg gcgttgtcga agcggcgggc acggaaaaaa
     4381 taattgccga agcgcgatac gcccgcacga aacaggacgc ctttgccgat gtcgcgttta
     4441 ttgtggatga ggattatcag aatatgggga ttgcctcgta tcttttcgaa ctgctgatcc
     4501 gtgcggcgcg cgaagagggc atcaagggat tcaccgccga cgtgctggct tccaataaat
     4561 ccatgctgaa agtttttgaa aaagcgcctt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nccgacgtgc tggcttccaa taaatccatg ctgaaagttt ttgaaaaagc
     4741 gcctttcccc gtgcagaccg ttctgtcccg cggcatttac gaactgacca ttccttttac
     4801 tgatgcttaa cggacgggca cggcagcaag accggcgacg ggctatcggc gttttttgag
     4861 atagccctcc aggcggttca ggccttcttg gatgttttcc agggaattgg catacgagaa
     4921 gcgcaaaaat ccttccccgc cgctgccgaa atcgatgccg ggcgtgacac cgaccttgac
     4981 ctcttcggta atacggaaag cttccttgta tgaatccttg cagaaccggc gcgcatcggc
     5041 aaacacgtaa aaagcgcccg tcggttcgac gtgaatgacg aaacccatat cctgcagacg
     5101 ggatagcata taccggcgcc tttcatcgta aatccgcacc attctggcca catctttttg
     5161 tgcctttgtc agggcggcga tgcccgccca ttgaataaac ccgttggcgg aaatcataaa
     5221 gttctgatga atgcgctgca tgactttgga aaacgttttg ggaaagatac agtagcccag
     5281 ccgccagccg gtcatcgcat acagcttgga gaatccgttg atcacgaacg ctttgtccgt
     5341 gaattccagg atggaatgcg cgcggtcttt gtaaaccagg ccatgataaa tttcgtcgga
     5401 gataatatac tgtttgtcga actgagcgac attctgcaat tgttcttcgg tcatgacgat
     5461 gccggtcgga ttggaaggcg aattgatcat aatcgctttt gtccgtgaag agagtttctt
     5521 tttgatgtct ttcggacggt attggaatcc gtcttcggga taggttctga cctcccgggt
     5581 tttgcctccg gccgcgagaa taaaattctg gtaacagggg taacgcggat tggaaataat
     5641 gacctcatca cacggatcaa ggatcgccag catcgcgaaa agcaaagcgg gcgacgtgcc
     5701 ggtggacacc aggacctgat cgggtgtaat gctgacgccg tattccagat aatagaaatc
     5761 gcagatggcc tgtctgagtt ccagcagtcc cagcgcgtgc gtgtacaggg ttttattttt
     5821 ggccagtgcg tccctggctg ccttcataat gaccggcggc gtcgggaaat caggctcccc
     5881 cacttccata tggatgactt tttcgccttg acgttccagc tcttcggctt tcgccataac
     5941 atccatcaca atgaaagacg taaatttgga cgctcttttg gaaatcatag atacccctcg
     6001 tttgtgatag ggtacctcta ttttgattgt ataaaaattt caacaacttt tctttgccgg
     6061 gagtggatgc tctccgcagc acgctgcgat gaattttcga cgggcaggac attaatatca
     6121 atttaatgtc gatttaaccg tctgctgccg gacgattaaa tatctttgaa agaaaaaacg
     6181 gggcacggcc ggacattcaa acttgttctc tttcgcagtg ccgaagaatc ttctttacaa
     6241 aataatccaa tttgttaccc taagtgctat taaaaaaaga aaaatgaata acagagatcc
     6301 ttcagatgag aacagtatta atcatcatcc tgattgccgt catttcttta ggaggctgca
     6361 acatgcaaaa ccgctttctt tattttccgg atgccacatg gccgacaaaa caggcgctgg
     6421 caaatgaaaa catgaacctc tggcaggcga caggagatga ttatcagggt ttggtttccg
     6481 ccatcgatgc gccggcaccc agcggcacca ttgtgctttt tcacggcaac ggcggaactg
     6541 ccctggacag agggttttat ttgccccctt ttatgaacct tggattccgc gttattctcg
     6601 ctgaatatcc aaagtacggc ggccgtcccg gtaaagttgg agaaaaaccg tttgtcgccg
     6661 ccggcctgga gacggtccgc ctggcccgcg agcactacgg agatcctctt tatctgggcg
     6721 gcgaatcgct gggctgcggt gtggttgccg cgattgtccg acagacaccg gttcccgtcg
     6781 ccggtatcat gttgataaca ccgtgggata cgctggcatc tgttgccaaa tcgcttttcc
     6841 cttttttccc ggttaaactg gtattgactg atcaatacga cagcatggaa aatttgaaaa
     6901 catttaaaaa taaaattgcc gtcgttggag cggagcggga tgaaattctg ccggtcaaac
     6961 acgcgataaa tttatataac gcccttcccg aaggccggaa acgactgtgg atcatcaaag
     7021 gtgcgggcca taacaattgg ccgatgttta ccgacgtttc cctgttcagg gaaatcgtcg
     7081 attttgtgaa aattgatgta catcaatgaa aagaagtttt ttccacccta tgatttgtcc
     7141 atatcatgta acggagaaat cttatgaaac aagagaagaa cttgaaaatc ggccgccggt
     7201 cttttctgaa actgacggcg ggagcggcag cgctttccgg tatgagtctg ccgaaaaaat
     7261 cttttgccgg gacagagcaa cgcttatcaa cgttgattga tctgaatctc tgcgacggct
     7321 gtgtcgatcg tgaaattccc gcctgtgtcg gtgcctgcaa agaaatcaac agggataaga
     7381 ttccgcaggt gatcgatccc attcccgaac cctggccgag aaaaaccatt gaagactggt
     7441 cgcacaagcg cgatgttgcc gatcgtttga ccccttacaa ttttatttat gtacacaagg
     7501 ccgacgtcag ggtggccggt caggcaaaaa caatctttgt tccgagacgc tgcatgcact
     7561 gcgataatcc ggcttgcgcg accatctgcc ctttttccgc gaatcacaaa cataaaagcg
     7621 gcgcggtggt catcgatcag aatctctgtt tcggcggtgc gaagtgccgc gatgtctgtc
     7681 cgtgggaaat tccccagcgg caatccggtg tcggcattta tctcaatgtt ctgcccggcc
     7741 tgatgggcaa cggcgtcatg tataaatgcg atctgtgtgt tgatcgtctc acgcagggca
     7801 aactgcccgg ctgtattgag gcctgtccgc gggaagcgat gctgatcggt tccagagcag
     7861 ccattgaaaa agccgccctg tcccgggccg cccgcatcaa cgggtatcta tacggcaaaa
     7921 cacaaaacgg cggcaccgcc acgctttacg tttctccggt tccttttgag gaaattaata
     7981 aaacaatgat aaagaagccg gggcagccgg acatgaaaat gaacgtcgag cgtagaatgg
     8041 cgggaacgga tgctctgggg aaggcggttc tggctgcgcc tgttctgggc ctagcggcgg
     8101 ctgccgtcgt tggagtgtgg ggacggatca tcagccgtaa agaaaaagcg ggcagggagg
     8161 aatgagacca tgaccgataa aattctgaca caagaaaatc gtgttgtccg ccacggcgcg
     8221 atggaattga tcgagcactg ggccatcgcc ctctcgggcc ttatcctgct tttaaccgga
     8281 atatttgagc tgcccgtcgg caaacgctac tatattattg acgttccggg tctgggctgg
     8341 tcggcggact ttataacgtc actgtacctg cattacgcgg cggcgctggt gtttacggca
     8401 gcggccgtgt tccatgtttt ttatcacggt ctgcggggcg aaaaagggct gcttccgcga
     8461 aaaggcgatt tgtccgaatc ggtaaccgtc atcaagagtc tgtttggatt cggtgaagag
     8521 ccgcccatgc gcaaatatct gccggagcag cgtctggcct acgccgcaat ggccttgatt
     8581 attctgatgc tgatcatatc ggggatggtg aagacataca aaaatattta cgcgccggat
     8641 atgtcgcatg gcattttgct ctgggctaca tggatccaca atatcggttt tatattgttc
     8701 ttcctcgcgt ttctggccca catggccgcc attgcgctgc ggcccaactg gccgatggtg
     8761 cgcgggatct ttaccggcag ggtgcgtctg gattacgcga ggcatcgtca tccgctctgg
     8821 attgccggaa tggaacccga accacaggat ccggcgacgg ttgaggaagg cgaacaacag
     8881 ccgctgccat ccgaagaagc gccggtgatg gaagaaacgg aagaagagga tctgcctccg
     8941 ggaaaaccgc agccggaaga agacaaaaca taaaactaat tcctccgtct tcctgtcggc
     9001 cgcccggttt tttttgtggt ggaaaacacg ccaaagacag gatataagcg tcataccgtc
     9061 attgaggagg aaatacccat ttaccgccgt atcagtctgt ttcgggtgct gcacatcagc
     9121 gccctgatga tctttatcct gctggcgctc agccttgtcg ctgtttctgt gggaagtgtt
     9181 tccatcgaac cgggaagggt tttaacgttt tttttcggac ctttgacggg gcaatcgcct
     9241 ttatcttcag aagaagaatt gattatctgt tcggtgcgtc tgccgcggat cgtttttgca
     9301 gggattgtcg gcgcggcgct ttcgctgggc ggcgttgttt ttcaggcgat cttgcgcaat
     9361 cccctggccg atccctatat cctgggcatc tccggagggg cagcgcttgg cgcgattatc
     9421 ggcattgtga tcggggccgg tgctttttat ctggggatac cgctgctggc tttcgcgggc
     9481 gcgctggcga cagtttttat tgtattcatg atggccggaa aaaccagagg ccccctgctg
     9541 gataattcgc ttttgctgtc cggtgtcgtt gtcaacgcct ttttttcggc cgccattctt
     9601 ttttttctat ccattgtcaa cagtatggaa ctgcacagca tcacgttctg gctgatgggc
     9661 gatctttctc acgcttcagc cagggaaatt cttgtcgccg ccctctgcct gctgtccggt
     9721 tttgttttgc tttacgcgca ggcgcgaaag ttgaatctga ttgtgcaggg ggaggaaacg
     9781 gccgaacatc tgggtgtgca tgtggcaaga acaaaaaaat ggctgatggt gattacctcg
     9841 ctcatgattg ccgtagcggt ttccctggcc ggcatcatcg gttttgtggg aatgatggtg
     9901 ccgcatctga tgcgtcttgt tttcggttcc gatcaccgtt tgattctgcc ggtctgcgcg
     9961 ttattcggcg cctcgttttt gattgcggcc gataccgtcg cgagggtgct cttggcgcct
    10021 gcggaattgc cggtcggcgt tgtcacggcg ctctgcggcg caccgtattt tatttttctt
    10081 ctgaaaagaa agaggctttg atcatgtctg ttattacagc cgaaaatatc cgtttcagtt
    10141 atccggggaa accggtgatg aacggcgttt cgttttccat tgaagcggcg gaaatggttg
    10201 cggtgatcgg ccccaacggt tccggcaaaa caacgctttt gaaaatgatc aacggtacgc
    10261 tgtttcccga tggggggcgg atgctgatcg acggcaggga aacgacgggc tggccgcgca
    10321 aggagatggc tcgccaagtg gccattgtgc ctcaggaaac ggcggtgacg ttcccgtttt
    10381 acgcggaaga aattgttctc atgggaaggt ttccgcattt gaacaattac cgttttgaag
    10441 ataagaagga ttaccggatt gtccgggagg ccatggaaaa aaccgatacc atttcttttg
    10501 ccgcccggag gttggatgaa ctcagcgcgg gagaacgtca gcgcgttctg atcgcaagag
    10561 ctctggccca ggagccgaaa attcttctgc tggatgagag cacggttttt ttggacctga
    10621 agcatcaggc ggagttttta tccctgttga ggcagttgaa tgccgtgcag aatctcaccg
    10681 tgatgtttgt cacgcacgac attaatctgg ccgcgcaaca cgcccataag ataattttgc
    10741 ttgattgcgg gaaaatttat gctataggaa tgcccgcgga agtcattacc gcggcgaaca
    10801 tcagagaggt ttatgacgtc gacgttttcg tcgaccgaaa tccgcatgac ggatcgcctc
    10861 gcgcaacact attgacaaat tgaatattgc tggtttgatt tcaaagggca accggccggt
    10921 gatcccgcat aaacgggaga tccgccgccg tctgcttcag aaaacaaatc aggaaaggct
    10981 gccagataaa ggaagccggt taaagtccgg cgctgccccg caactgtaag cggaacgaaa
    11041 ttcacattga tccacggacg ttttagcgtc cggaaggagt gaaaagtagg tcgccgcaag
    11101 ccaggaaact ttacggtgag cacaacttac tttgatcccg agggggaaag gagaagctcc
    11161 atgaagaaga ttcttttttt tgttctgttt ttttccgtag ttgttttttc acccgcaaac
    11221 gcttcccaga aggatgccga caatgcgctg gtcacgatgg gcgaaatcgt tgtcactgca
    11281 acacgcgatg cgcaggaaat tagaaagaca ccgtcgagcg tcagcgtaat taccgaagag
    11341 gagatcaacg attccggtgc gacgaccctg gtggaagttc tggacagaat cgagagtatt
    11401 gcgtttagga cgtacagcgg gaattcgtcg cagtcgcaga tcgacatgcg cggattcggc
    11461 ggagataatc ctttcggcaa aacactgatt ctgctggacg ggcgccggtt gaaccggacc
    11521 gacatggcct ccatcaactg gttgcagatg cccgtcaacg gcgtcgagaa aatcgaaatt
    11581 gtccgcggac cgggaagcgt tctgtacggc gatgcggctg tcggcggcgt gatcaatatc
    11641 atcactaaaa aaggaaaagg gaaaccgacc gtcaacctgt cggccatcgg cgggagttac
    11701 ggtctgcacg atgaacggat cggtgtgagc ggcgctaccg gcaaatggac ttacgctctg
    11761 acgggcgaaa acaatttcag ctccggatac agggaccgct caaagtattc ggcgcgcggc
    11821 ggagggctgg atgtgggata ctttgccggg gatatcctga acatgtcgct gggcgtttct
    11881 tttaaccggg tggattatca gatgcccggc gctctgacca aagcccagat ggtgaaggac
    11941 aggaggcagt atcagcccgc catgccggat tattacatga atgccaacga tgataacgac
    12001 ggatccgata agtacaccaa tatcaagttt gatgtgaaat cgttctgggg ggcctgggga
    12061 agaaccgaag tcaattttct ctatgggaaa aaagatcttc agatgaacat gccgtcatgg
    12121 ttttcatata attattccga caccgatgcc gagacattcg gcatgacgcc ccggtatatt
    12181 ttggagaaga atattttcgg ttttcacaac aaagtgatca tggggctgga ttattatcat
    12241 gaaccgtacg cgaaggatat tttcagcaat cgcagaagga cggaccggtt aagcaccgcc
    12301 gactttacgc gggatgccct gggatattac atccgggacg aattcagcgt gttgaaaaac
    12361 ctgattctca gcgccggtta ccgtttcgat cgcgtgtcgg tcgaagggtc caatgaagat
    12421 ttttcgacgc cgtccaataa ttttacaaat cagaagaata tttataaagc cgaagcgtat
    12481 gaggctggac ttacgtggct ctggggcgat cagtcgaaaa tattcgccaa gtacgccacc
    12541 gtttaccgaa ttccttttct ggatgaagtg gcgtcattca gcggtttcgg agggaatttc
    12601 ctgaagagtc tggaaaaaga aaaagggaaa agcatggaaa tgggcgcaga gtattatctg
    12661 ctgaataatc tgaaactggg ggttgctctg ttccgtttgg acatggagga cgaaatcgaa
    12721 tatgtttacg atcctgaaac gtttacggga gaaaaccgta ataccggtaa gacacggcat
    12781 gacggcgtgg aagcgtcctt tgcctatctc ttgcagaagt atttcaggat atttggaaat
    12841 tacacgtatc acaaggcgac ctttgaaaag ggcctctaca acaaaaaaga aatgccgatg
    12901 gttccccggc acgcggccaa tgtcggtctg gaaatatacc tgcccggtca tgttacgctc
    12961 agaacggaag ttcaacatgt gggcaaagcg tatctttccg gcgacagcga caattctact
    13021 gaaaagttgg aagacaggac attgctgaat attgttctcc agtacaaacc ggtctgggat
    13081 acaatgaaat tgaccgcgtt tctgggcgtg gataatgtcg ccgatgtgaa gtattcgtct
    13141 ttcggtatcg attatgaaca gtacgccatg cccaattttt attatccgat gcccggcgtc
    13201 acattcaaag gaggcgtttc ggttgagttt taagcatctg tctttcaaag acaatgccgt
    13261 gatcgtattt tccgttttga tattggcggt attttgcgcg ggcttggcat gcgcgcggac
    13321 ggtcaccgat gaaatgggcc gcatcgtcca tgtccgtcgc gtgccgcaga gaatcgtgtc
    13381 ccttgccccc ggtattacgg aaacccttta tgcgttgggt ctggaggaca gaatcgtcgg
    13441 cgtaaccacg ttctgcgact ggccgccaaa agcccgcctc aaaggaaaag tgggcgggtt
    13501 taccaatccg tccatagaaa aaattgtttc tttgagaccg gacctgatca ttgccactgc
    13561 cgacgggaac aaaaaagata ccgtagaaaa actggaacgt ctcggattgc cggtgtatgt
    13621 tacaaaaccg tcggataccc ggggcgttct ggaaagcatc atgcggatcg gggaaataac
    13681 cggtcgcacc aaggaagccg acgaactggc agacgggctg agaaagcgaa tgaatagaat
    13741 aaccgcccgg atacgcggta aaaacaggcc gcgcgttttt tttcaactgg ggatggaccc
    13801 gatgataacg gccggccgga atacgttaat tcacgaagtg attgagaaag ctgggggcat
    13861 caacattgcc ggacatgata cggccaatta tccccgttac agcacggaag ggatcatggc
    13921 cgcatcgccg gacattattg tttttgcgcc gatggtcaat gatcaggaat tttcgattgt
    13981 taaaaaattc tggggcagat tcgggaaaat accggccgtg caaaacaaca gaatctatct
    14041 gatagacgcc gacctgatca acagggcttc gccgcgcatt tttgatgccg tcgaacgcat
    14101 agcgttgatt tttcatcctg atataaaatg ttttcaataa tgaatttctg ttgtacgatc
    14161 cgccacggcc tttgatccgg aatgatcgtt gaagtatttc cggggaacat ggagccgacg
    14221 gtttgttgaa tacaataaac ggaaaagatc aaaacagtga tgattgacgg caaactgcca
    14281 accagaggcc tggtctatgc ggcgcttttc ggagccatga cggcagcggg ggcgttgatt
    14341 gttattcctc ttccgcccgt gccggttact gcgcagacat tttttatgaa tgtcgccgcc
    14401 cttcttcttg gcggtccgtt ggcggttgtc agccagataa tttatgtctt gctgggcgtg
    14461 gtgggaatgc cggtttttgc cgggggcaaa gccgggctgg gcattttgtt cgggccaacc
    14521 ggcggttatc tattcggttt tatcattgcc gcttttgtca tcggccatgt ccatcgcatg
    14581 aaaaaaaacg ccggcgcggt ctggcaggta ttttccatgg tgctgggcat gctggtcatt
    14641 tatgttgcgg gaagcattca attggcatgg attgcgaaca tgagtcttcc gcaggtgctg
    14701 atcgtcgggg tagtaccttt tatcccgggc gatcttatca aaatagcgct ggctgtcctg
    14761 atcagttccc ggttaaaaaa ccgcatagag atttgaggaa acgacgttga ttgttctgga
    14821 agatgtttat tgccggtatg aaccggaagg cattgaagcc ctacgcggcg tttctctgca
    14881 aatccgggag ggagaacgtc tggctttaat cggtccgaac ggctgcggca aaaccacatt
    14941 gatcaggcat tgcaatgcgc tgcttgtgcc cgaaaaaggt catgtcaccg tcggcggatt
    15001 aaggacggat cataaagaga accaacccga tatccggtca cgggtgggca tggttttcca
    15061 gaatcccgat catcaaatcg tcgggatgac ggtcgaagac gatgtggcat tcggtccgga
    15121 aaatttgcgt ctgcccgcgg cggagatcaa ggcaagggtt gccgaatccc tgacccgggt
    15181 gggcatttac ggacttgccg cccgtaatat cgaagatctt tccggcggcg aaaaaagact
    15241 ggtggcgatt gccggcgtgc tggctatgcg gccgcgttat attgctctgg atgagccgac
    15301 cgcctttctg gatccggcag cctccggaag ggtgctggcg attatcgaca aactgcatgc
    15361 cgaaggcgtg ggaatcattc acatcaccca caacatggcg gaagctgccc gggcgcaaaa
    15421 aatcgtcgtg atggatcagg gccaaatctg catgcagggc acgccggagg caattttcag
    15481 ccggtttgaa atcctgcaaa aaatggggct tcagcttccc cagattacgg aacttctcat
    15541 ccggctgaag caaacgggct ttccggtccg aacgggcatt gtccatatgg aagacgcctt
    15601 aaaggaaatt atttccatga ccgcgcgtcg taaggattga acatgtaccg caccggttcg
    15661 tttatcaggg gagagtcgtt catttaccgg ctggatcccc gcctgaaact cgctgtaacc
    15721 gtcttgttga gtctgttgat ttttcaggtc gaacaaccgc tggtttttgc ttcaggcatc
    15781 ggtttgtttt tgctggctat ggccagcggc atacgtctgc ggacaatcgg tgagtcgatc
    15841 agaccggtcc ttttctttat cgtcctgatt tttctgtcgc atcttttatt cagcgacgat
    15901 aaacgctcca tgatgacgat cccttttttg gggattgcct tttcgtctgc cggattaaaa
    15961 cagggaaccc ttgtggtcgg acaattttta tgtctcattg tcggggccgt tttgttgacg
    16021 atgaccacgg caccttccca gatcattgcg gcactgagat attttttaaa acctttcaaa
    16081 aaactgcggc tgccggtgga tgacgttgcc gtgatggtcg cgctggcttt aaggctcatg
    16141 cccctgttgc tgatggaaaa agaacgaatc gatgccgccc ggaaagccag agggttttgt
    16201 ctgcggcgac agagtgttgc cggccggatg aattcctttt tgtctttgat ttcaaaaatg
    16261 ctgctgggca ttttccggaa agcggacgat atcgccctgg ccatggaagc ccgaagctac
    16321 cggcgtggcg ggcgttcgtc ctttgtggaa ttgaaatttt cgtccgccga ttatgttgtc
    16381 cttgtttttt tggcaatttt tctggttatt tttgtggcat taaattcgca tttcggttaa
    16441 attacccgcc atgaatgcga aatggttttt ggtcgataca caaaacaaac tgaagcgcgc
    16501 cgttgctgat ttcgagcggg caaaaattct cagtatcgat acggaatacg attcgtttca
    16561 ttatttcagg gaaaaactct gtctgattca gatccatgcc aatcacacga cctacctctt
    16621 tgatccgttg ggcgatgtgg ctttgtcttt tttaggcgat ttttttgata atcagcgtat
    16681 tgtcaagatt cttcatgctg cggataacga tatccgtctt ctccggagag attacggatt
    16741 cggtttcaga aatatttttg atacacaccg ggcggcgttt cttctgggtt tccgggagct
    16801 gtctctggac aaaatgatta aagaatttgt cggcgtggaa ttgaaaaaaa acaaaaaaat
    16861 ccagcgttcc cattgggata atcgtccgct gacggaggaa caggttgaat acgccgtcca
    16921 ggatgtgatt taccttcccg atctttatca aaagcagacg gcggaactga ccgccaaaaa
    16981 tcttcaggaa tccgcgcggg aagcgtttgc cagaattgcc gagacggact ggcgggagaa
    17041 aaaattcaac cggtgggggt atgcaaaaat aaaaggttat aatgccctca atcaggagca
    17101 gaaggatctg ctgaagaagt tgtacctatg gcgttttcac cgggcgaagg aagaaaatcg
    17161 cgcggtcttc atgtttctgc cggataaaaa tcttttggat ctcgcgctgg atgcggatca
    17221 tcccgaaaaa tatttatcac aaaggaagat gcgaatttac ggttcggaaa tcgaaacgat
    17281 tataaaaagg atgtaatcat ttgaaattcg atcctgagga tgtttccagc ggcaaaaaag
    17341 ccttcaaatg ccgatctgtc tttcgtagag gtggcccgaa aataaccgga ttatcaagtt
    17401 gttgtcgaaa gagatttgat aacggtcttt cgtgttttca aagcgggggg ggggaagcgt
    17461 gacgggattc atttgcattt aactttaaag cccaccggtt tattgttttt aaaatagcct
    17521 gtaaacgtct tgccggtgct gcaggtatag gttccccggc cttcgatttt gccgtttaca
    17581 aagtcgcctt catatttgtt gccgctcgga gaggtaatct tgcccttgcc gtgtaatttg
    17641 ccgttgataa tctgcccctc gtatttattg ccgttggcat cggtatacga cccatgtctt
    17701 ggctgggcct tttgacggct gtccggaacg taatacacat tgccgctgca gttgatcgta
    17761 aaacccaccg gtttgttatt ttcaaaattg ccggtaaact cgcggccgtt tttgcaggtg
    17821 tatttcccct tgccgctcat ttttccttca aaccagtcgc cctcgtattt gttgccgttt
    17881 gtccagttgt aagttccccg gccatgcggt ttgccgtttg ccattgttcc ttcataacgg
    17941 gtaccgtcgg caaaggtgta acgctctgta ataccggaac tttcagaaac cgatttgact
    18001 tctttcaatg tccagccccg tgtttccatg cagcggttat acatatccac attggtcgac
    18061 gcgctacccg tcgcctgggc ttttttcata cagtcatcca tgtcttttgc aaaatcctgc
    18121 tgtgcggcat tcggctttga ccagactgcc ctggtggccg gcgtcgtggc gcatgctccg
    18181 atgaaaacga tcatcagaaa catgacgagc atcatcagcg atgccgtttt ttcttgagtc
    18241 atggcaagct ttttcatgtt tcccccgaag attgggtttg ctgaagaatt aacattgttc
    18301 agcaagacga tgataactgc tgaatgagat tctggtggag atgaggggga tcgaacccct
    18361 ggcctcgaca ttgcgaacgt cgcgctc
//
LOCUS       DAONQZ010000045        17853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3560_length_17853_cov_13.601023, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000045 DAONQZ010000000
VERSION     DAONQZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17853
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3560_length_17853_cov_13.601023"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..917)
                     /locus_tag="PLR13_08055"
     CDS             complement(465..917)
                     /locus_tag="PLR13_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQC19329.1"
                     /translation="MKNSKGVPPQPSSRQHKDNIQELKSEDALEALQGLNPVTFTYNE
                     EALSKGVPPPDACSKHFKDNINYLNKEDAVKAVTALNPVTFTYKECPQEQRVGFIAED
                     VPHLVATNDRKGLYAMDIVAVLTKVVQAQQKTITDMRDELNELKAKVM"
     gene            1401..3371
                     /locus_tag="PLR13_08060"
     CDS             1401..3371
                     /locus_tag="PLR13_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQC19330.1"
                     /translation="MIDYAKELNPEQYRVVMEEGGPLLVLAGAGSGKTRTLTYRVARL
                     LESGVAQENILLATFTNKASHAMLNRVESLIHAETRNIMGGTFHHVAHLILRRHAHYL
                     GYKTNFSIVDSEDARQIISTCMAELKIDPKLSKFPKSNIVGDIISLSVNTETTVENVL
                     MSRYPFFLHLMDEIIQVASLYEQKKRELAVMDFDDLLSNCRMLLQENADVLKNLSGRF
                     QYVLVDEYQDTNLIQADLIDLLASTHKNLMVVGDDSQSIYSFRGADFNNIINFPHRYP
                     DCKIFKLETNYRSTPEILHLANLSITNNEKQFPKELKAVRKNGMRPVIVSAQNVVKQA
                     DFVAQRVTELMRGGVPLREIAVLYRAHYHSMEMQMEFVRRDIPFDMRSGIRFFEQAHI
                     KDVTSYMRVLVNPRDELAWRRLLIMYPKVGKATFNKIWHYLKKEDNPLNAVLSEDFIK
                     AMSKSAKPGLEECRKTIRQLLDLPENERIPERIIDVLLNRGGYRVYLQDNYSDASSRE
                     EDLIQLGNFSAQFLQMEDFLNELALLTNMAKEENIGEEDDEDKVALSTIHQAKGLEWS
                     YVFLIWCADGMIPLQKALKEQDGEEEERRLFYVAVTRAKDQLYLCYPALDYTRSSGVL
                     NMAPSRFIREIAPLSEYDADRPFEQWMLCDDY"
     gene            3556..4914
                     /gene="trkA"
                     /locus_tag="PLR13_08065"
     CDS             3556..4914
                     /gene="trkA"
                     /locus_tag="PLR13_08065"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQC19331.1"
                     /translation="MRVIIVGAGEVGYHIAKKLSDEKRDVVLIDKDPGKIRRINENLD
                     VQAFLGSGTSPKMLRQAGIQDAQLLVAATDMDEVNLIACLLARNLNRYAVKIARVRNQ
                     EYLQEKSLFSKDLLGVDLIINTQSMMVETILNLMDIPGASEVIDFLGGAVKLVSFLIF
                     EDSPLAGKKLSELSNQEGKLLVGAIVRGHKILIPNGNDTIQPMDLVYFVIKKSELDQT
                     LSRLHMPAKPMRNVIIVGAGGTGMAVAKALDQRRLNVKLIDQDGAKCKEANRLLEKVV
                     VIKGDGGDRDLLIEENARAMDMLIAVTDDEESNVLISLLAKELGTARTITRVSKLSYM
                     HVVSNVGLDTVINPRMAAVRAILQYIRRGKIISVAPLREEQAEAIEAEALETSDIVNV
                     PLSKVDFPPGAIIGAILRGDAIVIPTGSTVVLPHDHLIIFAVQKVINKVEKLLTVKLE
                     YF"
     gene            4914..6362
                     /locus_tag="PLR13_08070"
     CDS             4914..6362
                     /locus_tag="PLR13_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQC19332.1"
                     /translation="MKTGLIVYMIALLVFYLGVSMFLPLFVALVYQDGSAFAFFVSIL
                     IACCLGGVVYLFTRGQKDFHLTHRDGLAIVTFGWIAAGFIGTLPYLLSGSIPDFTMAY
                     FESVSGFTTTGASILKDVQHLPPAVLFWRAQTQWMGGMGIIVLSIAILPFLGIGGMEL
                     YKAETPSPVVDKLTPRISSTAKLLWKIYIFLTLFLIILLLLGGMPLYDSICHSFAAMP
                     TGGFSTKNVSVGYYQSSYIDYVILLFMFIAGVNFSLHYKLVSGHPKQFFRDPEFRVYL
                     YITVIAILVVSANIYGTVYPRIQDALRYASFQVVSIMTTTGFVTADFEQWPVFSQLIL
                     FVFMFLGGMAGSTAGGIKIMRIILLVKHCYLEIFRIIHPHATTMVKLGRTPVSQTIMR
                     SIWGFFILYLVIFMIGAIALAALGLDIISALTAMATCLGNVGPGLGSVGPMDNYSVIP
                     VTGQWILIVSMLLGRLEIYTIIVLLMPAFWRK"
     gene            complement(6482..8374)
                     /locus_tag="PLR13_08075"
     CDS             complement(6482..8374)
                     /locus_tag="PLR13_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HQC19333.1"
                     /translation="MAKIKKENKPTAAQKSKIKKAALVDVDQPNLYKEIFPYSEVSKI
                     VFDHQTVPMEPAQEIFITDTTFRDGQQSRPPYTAEQIASIFQLLSRLGGPNGVIRQSE
                     FFLYSKKDKDAVEKCMALNLRYPEITSWIRASKDDVPLVKQFGLKETGILTSVSDYHI
                     FLKLNKKRSQAMNDYLGIVKTILDLGIIPRCHFEDVTRADIYGFCIPFAQQLMKLREE
                     SGIDIKIRLCDTMGYGITYPGAALPRSVDKLVHAFIHDAGVPGHLLEWHGHNDFHKAM
                     INATTAWLFGCSAANSTLLGLGERTGNPPIEGLIIEYIALKGRNGIDTTVITDIADYF
                     EKEIGVKIPHNYPFVGSGFNVTSAGVHADGLIKCEEIYNIFDTRKILKRPITTTITDK
                     SGNAGIAFWINQRFNLEGNKTVDKRHPGVSKIHKWVMEQYESGRITSISPEEMEKKVR
                     LYLPERFMSELEKIKYIAEKAAVAAVTHIIEQPLMKTMDTAKQEPLMQKFVDEYPSVQ
                     FAYVVDMNGRKTTRNITDIADRAKYENYGVGTDQSDRQWFINPLRTGEVYVSDFFISK
                     MTGVLCFTVSAPIFNTDDEMVGIFGVDIKFEDWVKRAEDMEDMDHIALHEEYKEMKSK
                     AKHGHH"
     gene            complement(8475..10244)
                     /gene="aspS"
                     /locus_tag="PLR13_08080"
     CDS             complement(8475..10244)
                     /gene="aspS"
                     /locus_tag="PLR13_08080"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQC19334.1"
                     /translation="MAEFLTTDKRTHYCGNLDASLIGQDVVLMGWTHRRRDHGGVIFV
                     DLRDREGMVQVVFNPDNAKAHKEAHRIRSEFVLAVKGKVRRRPDGMENPSLKSGEVEV
                     LVSDLDIMNESKTPPFSFDDEEISENIRLKYRYLDLRRPAIQKNLFLRSRMAAAARRY
                     FEENGFIEVETPFLGKSTPEGARDYLVPSRIYKGMFYALPQSPQIFKQLLMISGFDRY
                     YQIVKCFRDEDLRADRQPEFTQIDVEMSFITEDDIMQMMEGLMKAIFKSCLDIDLALP
                     LPRLTYAAAVSRYGKDNPDVRFGMEIVDLTSIVKDSGFKLFAEVASSGGVVKAIKAKQ
                     ASVLSRKDLDGLKDVVAVYGANGLAWAKINAADWTSPVFKYLKPDEVQAIGNALDARE
                     GDIIFFIADKPKVASDALGNLRLHLGKKLGLIDPKALAFTWITEFPLMEYSETEKRFV
                     STHHPFTSPFLEDLPLLTADPGKVRARAYDLVLNGSEIGGGSIRIHHKDVQSQVFSAL
                     KLSDEDARLKFGFLLDALEYGAPPHGGLAFGLDRLTMIMTGAESIRDVIAFPKTQKAA
                     CLMTDAPSKVSIEQLMELSIKIV"
     gene            complement(10251..11513)
                     /gene="hisS"
                     /locus_tag="PLR13_08085"
     CDS             complement(10251..11513)
                     /gene="hisS"
                     /locus_tag="PLR13_08085"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQC19335.1"
                     /translation="MEKITAIRGFKDILPDDALAFCRIESLARTIFDSFGFREIRVPI
                     LEKTELFKRSIGETTDIVEKEMYTFTDRDNDQITLRPEATASVIRAYIEHNMSANEPV
                     TKLFTFGPMFRRERPQKGRFRQFNQIDVEVFGDDKPQTDAEVIFMLMHFLNSAGLRNL
                     QLEINSLGCSACRPEFSKAVIDFLKDRQSRLCPDCQRRIHTNPLRVFDCKVPSCAETL
                     AGAPQIIDFLCSDCTQHFAQVQSFLADLQTPFVINARMVRGLDYYTRTAFEVTTGILG
                     AQNAVAGGGRYNGLVSFLGGPDVTGIGFAVGFERLVACLPENRKDTYTTDLFIAALGA
                     NAQHIAFGLTNRLRVCGLSAEMDYSDKSLKSQMKRADKLNSAYALIFGDKEIEEKQVV
                     LRNMQTKQQQDIPMDGLHESIIKIIKER"
     gene            complement(11691..13448)
                     /locus_tag="PLR13_08090"
     CDS             complement(11691..13448)
                     /locus_tag="PLR13_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19336.1"
                     /translation="MNEVLRVLIVDDSDDDTNLVLRKLRSGGYQPEWERVDTHDAMKN
                     ALEKKTWDVILCDYKMPGFSAPAALKLAQEINPNIPFIVVSGAIGEETAVEVMKAGAH
                     DYVMKDKLAKLSVAVKREIAEAKIRRDKKSMEDMLKRSENNFRNSLDESPLGVRIVTS
                     DGELLYANREILNMFGYDSIDELKSISHLQRYTPETFVEYEKRIEMRKHGEYVPPNYE
                     VSIVRKDGTIRHLEVFRKQVLWNGQMQFQALYNDITERKQAEKNLRESEKRYRVVVEN
                     ANEAILITQNMKILFANTVAINNTGYPMEELTSMNFSDLIHPDDIGMVRGYHDRRIKG
                     DAIPSVYSFRIVCRDKSVKWVELNTTVIEWEKKRAALNFLKDITVSKQLEEERSQSFI
                     KIKKTLDATVSAIASMVETRDPYTTGHQKRVSILARAIAEEMGLCMDVVEFIETAAII
                     HDIGKLSVPSEILSKPTKLTNLEFELIKTHSQSGYNILKDIEFPWPVADAILQHHERM
                     NGTGYPQKLQGYDILLESRILAVADVVEAISSHRPYRPGLGIDFALEEITKNKGVLYD
                     ENVVDACIKLFREKHFVLS"
     gene            complement(13526..>17853)
                     /locus_tag="PLR13_08095"
     CDS             complement(13526..>17853)
                     /locus_tag="PLR13_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19337.1"
                     /translation="KDIQDCFAKKTTIKKELFSERFIPGTYIKASYSFVPPDLVMVHI
                     EDISERKQAEAALRESESLYRMLTEKMSDIVWTMDMSMRTTYVSPSVKKVLGFTPKER
                     LRQTVEEQLTPESLAAAVNIMDRELSDEKQGKSNPEKNVTLTLEYYHKNGSTRWLETT
                     VSGLRNEQGKLTGLHGLSRDITQRKRMEDELIESEKKYHSIVDNSTDAILLTIPDGDV
                     LSANAAACKMFGRTEEDIRRAGRPGVMDLSDPRLPAALEERKQTGRFRGELTFVRKDG
                     SKFEGEISSVIFTDRKGTPLTSMIIRDISERKQAEEALKQRDLLFKKLSAHVPGMIYQ
                     FVKKPDGTYCLPFSTDAIRTIFGCFPEDVAHDYSPILHAVHPEDVDDFLNSIEYSTQH
                     MTDWKHEYRVQLPDGTVKWMLGQSTPEKLPDGTIVWHGFNADITDQKNIQESLKATQD
                     NYRRLFEDHSAVKLIMDPETGAILDANFAAANYYGWTREEMKQMKISQINILSPDEIK
                     HEMDKVMQEHKVHFEFRHRRADGSIRDVEVYSSRVRRNGSYVLHSIIHDVTERKQAER
                     ELKQAQAYNEAILKSIPGSLYLIDENGRLVHWNKQFEILTGYDAHEIQDSFVLDWFKD
                     RQNDLLNIKAGIDTIMTRGSASNVEANLQTKDGRLIPMLYTGVRFDIGGKHHILGVGT
                     DITERKVAEEKLLREEKRFRSFAEQSSDIIALVDKNGTMLYENPAVEKLLGYKQEERI
                     GQDTFLNVHPDDLEDLMRCFQILFTDKNVPVQKTETRIRHKDGTWLTFEISGSNVVNN
                     DVIEFITVSLHDITERKRIQQELLESRNYAEKLLRTANALIVVLDTEGNVVSLNEAGE
                     KITGYRAGDIAGLNWFDLIVPKNRYPYVWEAFKKWKFSNDLPESMENPIVTKSGEERL
                     ISWRNSTIIEKGRFAGILAFGMDVTERKKMEESLQTWMRRYELIVAASGQVAYEYTLS
                     TGRIIWGSSIANVLGYDMGEISGGFGQWEILLHPDDRDATIQSLEAAEKACGFWESQY
                     RMKHRKGHYVWIRDRGFFVPDAEGKAYCQLGMLEDITSLKQAEEELKRTLLMNKAILE
                     SVPGILYLYNDTGHLVQWNRQFEVLTGYSGDELQGKYVMDWFGGLEPDTSNIKKGISD
                     VMTKGYSSAEARMITKNGDHLFMFFTGVKLTVSGQDYLLGIGLDISERKKAEMEIVRL
                     NESLEEKVQERTRELEAFSYSVSHDLRAPLRTIEGFSLALVEDYSDKLDNQAKDYISR
                     IRRATEIMSELIDDMLKLSRITRTEIDKADINLSNIALTVVDELRKAHPDRPVKVTMP
                     DSLTDYADSRLIKIVLENLLGNAWKFTGKKTDAEIEFGSTQQNGKKVYFVRDNGAGFD
                     MEYVDKIFAPFQRLHNIEEFPGTGIGLATVRRIISRHGGTVWAESVPHQGATFYFTLG
                     YPPPA"
BASE COUNT         4219 a         4244 c         4372 g         5018 t
ORIGIN      
        1 cctttcaatg ctttaaaaag acaattcaaa ttgaatttgg gctgtttaac gtataaggga
       61 agtaaaatca agacaaattt gatatttgct attccagaga cttgatgaac cgttcaggga
      121 agtgcttgaa tatgatgaaa ataatcaagg ggtgatggaa ttcatgtggt cgtgagctca
      181 atgttaggtt gcatgagcta aatacagact tgtctccttt cttgctttca cgatttgtcg
      241 gctttaaagg tcgagattaa aaatttaacc ccatattttt ccgacataag gcaatgaaat
      301 atatatccaa tctattatgg ataaaatagc ttattccgca aaaaatgatt tgccgttcaa
      361 tccggagacg ttgttgataa aagattgaaa tttaaaaagg cagaatcatt aatgatcctg
      421 cctttgaaat acttctcata tttcccttga agatgaaccg tattttacat tactttagct
      481 ttaagttcat tcaactcatc tctcatgtcg gtaattgttt tctgctgcgc ttgcactact
      541 tttgttagta ctgccacgat atccatagcg tatagtcctt tacggtcgtt tgtggcaaca
      601 agatgcggaa catcttcggc aatgaagcct acacgttgtt cctgaggaca ttccttgtat
      661 gtaaacgtaa caggattaag cgccgtgact gctttcacag catcttcctt gtttaggtag
      721 ttaatattat ccttgaagtg tttgctgcaa gcatctggag gaggaacgcc ttttgaaagt
      781 gcttcctcat tatacgtgaa cgtaacggga ttgagccctt gaagcgcttc tagggcatct
      841 tcggatttca actcctgaat attatcttta tgctgtctgc tggacggttg tggaggaact
      901 ccttttgaat ttttcaaagt tatctccttt catgtcttat tatttttaat atgttaaact
      961 ttaatattta tcaaaaaaag ggggccgagt atttgtttgg ctcttggctc atgccaacag
     1021 tttcgaattc ttgggccttt agaagcatat ttctgttcct gattatattc tgctttgttt
     1081 ttgtgatcac gctttccaca atacgggtag agagtgacag cagcgcctaa tcattaaaag
     1141 ctattttttg aaaacctgtg atgtcttttg gatttttaat atgctatttt aataaacaat
     1201 ttcatatttt ttgtcaattc tttttttaac tcagaatcct tcttttgcat cgggtttagc
     1261 gccgaatcta ttgtatgtcg gatgcggtgc gcagagaaat gcgggaacag ggcgagagaa
     1321 atatgaggaa gttcatatta tgtggacctt ttgccgttat tcgcttgaat atctgaacct
     1381 tttactataa agacgcgttg atgattgatt atgcaaaaga actgaatccg gaacagtatc
     1441 gtgtggtcat ggaagagggc ggtccgctgc tggttctggc cggggccgga agcggaaaaa
     1501 cccgaacgct tacttatcga gtcgcccgcc tgctggaaag cggcgttgcg caggaaaaca
     1561 ttttactggc cactttcacc aataaggcct ctcatgccat gctcaaccgg gtggagagcc
     1621 tgattcacgc cgagacgaga aatatcatgg gcggcacatt tcaccatgtt gcccatctga
     1681 ttttgagaag gcatgcgcat taccttggct ataaaacaaa tttttcgatt gtggacagtg
     1741 aggatgcccg acagatcatt tccacctgta tggccgaatt aaaaatagac ccgaaattaa
     1801 gcaagttccc caagagtaat atcgtagggg acatcatcag cctgtctgtc aacacggaaa
     1861 ctaccgtgga aaatgttctg atgtcacgat acccgttttt tctccatctg atggatgaaa
     1921 ttattcaagt tgcttctctt tatgaacaaa agaagaggga gctggccgtc atggattttg
     1981 acgatttatt aagcaattgt cgcatgctcc ttcaggaaaa cgctgatgtt ctgaaaaatc
     2041 tttccggccg gtttcagtat gtcctggtgg atgaatatca ggataccaat cttattcagg
     2101 cggatctgat cgatctgctg gcctctacgc acaaaaacct gatggtggtc ggagacgatt
     2161 cccagagtat ttactccttt cgtggtgccg atttcaacaa catcattaat ttcccccatc
     2221 gttatccgga ctgcaaaata ttcaagctgg aaacaaacta tcgcagtacg ccggaaatac
     2281 tgcatctggc aaatttaagc atcaccaata acgaaaaaca atttcccaag gaactcaagg
     2341 ctgtgcgcaa aaacggcatg aggccggtga ttgtttccgc gcagaatgtt gttaaacagg
     2401 cggattttgt ggcccagcgg gttacggaac tgatgcgggg cggcgtgccg ctccgggaaa
     2461 tcgccgtgct ttaccgggcg cattatcatt cgatggaaat gcagatggaa tttgtccggc
     2521 gcgacattcc tttcgatatg cgttcgggca ttcgtttttt tgaacaggcg catattaaag
     2581 acgtaacgtc ctacatgagg gttctggtca atccccgcga cgaactggcc tggcgtaggt
     2641 tgctgatcat gtatcccaag gtgggcaagg ccaccttcaa taaaatatgg cattatctga
     2701 agaaggaaga caatccgctg aatgctgtct tgagtgagga cttcatcaag gcgatgtcca
     2761 aatccgcaaa acccggtctg gaagaatgcc ggaaaacgat ccggcagctt ctcgatcttc
     2821 ctgaaaatga acggattccg gaaaggatta tcgatgtgtt gttaaacaga ggcggctacc
     2881 gggtttatct tcaggataac tattccgatg catcatccag agaggaagat ttaattcagc
     2941 tcggaaattt ttccgcccag tttctgcaga tggaggactt cctcaacgaa ctggcgcttc
     3001 tgaccaatat ggccaaagaa gagaacatcg gggaagagga cgatgaagac aaggttgcct
     3061 taagcaccat tcatcaggcc aaggggctgg agtggtcgta tgtttttctg atctggtgcg
     3121 cggatggcat gattccgttg cagaaggcgt tgaaagagca ggacggcgag gaagaggaaa
     3181 gaaggctctt ctatgtggcg gtgacccggg ccaaagatca actctattta tgctatcctg
     3241 ccctggatta tacgcgttcc tcaggagttc tcaatatggc tccctcccgg tttatccggg
     3301 aaatcgcgcc tctttcagaa tacgatgcgg accggccttt tgaacagtgg atgttatgtg
     3361 atgattatta agaaattgca cattcaaaaa agagcaatat gtttttaaaa atcctatcat
     3421 cctggtcact ctggaatccg aaaaagccgt ccgtaaagtg aatgatctgg ggcggcgtta
     3481 tcaagctttt tatgttgtaa ttcccggggt gtccattata taagttgata ccgttgtaac
     3541 gtggaggggg gaaatatgcg cgttattata gtgggcgccg gagaggtcgg ataccacatt
     3601 gccaaaaaac tttccgatga aaaaagagac gttgtcttaa ttgacaagga tccgggcaag
     3661 atcagacgga tcaacgaaaa ccttgatgtc caggcttttc ttggatcggg cacaagtccc
     3721 aaaatgttgc ggcaggcagg cattcaggat gcgcaacttc ttgtggccgc aaccgatatg
     3781 gacgaagtca atctcattgc gtgcctgctg gcgcgcaacc tgaacagata tgcggtaaaa
     3841 atagcgcgtg tcagaaatca ggaatacctt caggaaaaat cattattcag caaagatctt
     3901 ctgggcgtgg atctgatcat caatacccag tccatgatgg tcgagaccat tctcaacctt
     3961 atggatattc cgggcgcatc ggaggtgatt gattttctgg ggggcgccgt caagttggtc
     4021 agctttctga ttttcgagga ttccccgctg gcgggtaaaa aactcagcga gctgagcaat
     4081 caggaaggaa aattgctggt tggcgcaatc gtcaggggac ataaaatcct tattcccaat
     4141 ggcaatgata ccattcaacc catggacctg gtttattttg tcatcaaaaa atcggaatta
     4201 gaccagacac tctcccgtct tcacatgcct gccaagccga tgagaaatgt gatcattgtc
     4261 ggagcgggag gaacgggcat ggccgtggcc aaagcgctcg atcaaagacg tttgaatgtc
     4321 aaattgattg atcaggacgg ggccaagtgc aaggaagcca acaggcttct tgaaaaagtc
     4381 gttgtgatca agggagacgg cggtgaccgg gacttgctta tcgaagagaa tgcccgcgca
     4441 atggacatgc tgattgccgt aaccgacgat gaagaaagca atgtcctgat ctcgcttttg
     4501 gcaaaggaat tgggaacagc acgcaccatc accagggtca gcaaactcag ttacatgcat
     4561 gtcgtctcca acgtcggatt ggatacggtc atcaatcccc gcatggctgc cgtccgcgcc
     4621 attctgcagt acatccggcg gggcaaaatc atttccgtgg ctcctttaag ggaagagcag
     4681 gctgaggcca ttgaagcgga agctttagaa acatccgata ttgtcaatgt tcccttatcg
     4741 aaggttgact ttcctcccgg cgcaatcatc ggcgctattt tacgcgggga tgcaattgtt
     4801 atccccaccg gcagtaccgt ggtgcttcct catgaccacc tgattatctt tgccgtgcaa
     4861 aaagtcatca acaaagtgga gaaacttctt accgtcaagc tggagtactt ttgatgaaaa
     4921 ccggtttaat cgtctacatg attgcactgc tggtattcta tctcggcgtt tccatgtttc
     4981 tgccgctgtt tgtagcgctt gtgtatcaag acggcagcgc cttcgctttt tttgtttcca
     5041 tcctgattgc atgttgcctg ggaggcgttg tctatctttt tacaaggggt caaaaagatt
     5101 ttcatttgac gcatcgggac ggcttggcca ttgtcacctt tggatggatc gccgcggggt
     5161 ttatcgggac gctgccgtat ctcctttcgg gatcgattcc ggactttaca atggcttact
     5221 tcgaatccgt ctccgggttt acaacgaccg gggcgagcat tctcaaggac gtgcaacatt
     5281 tgccgcccgc agtacttttc tggcgcgctc aaacgcagtg gatgggcggc atggggatta
     5341 tcgtgttatc catcgccatt cttccgttcc tggggatcgg cggcatggaa ctctataagg
     5401 ctgagacgcc gagtcccgtt gtggacaaat taacgccgcg gatttccagc acagccaaat
     5461 tgctctggaa aatttacatt tttctcacgc tttttctgat tattttactg ttgctgggcg
     5521 gtatgccgct ttatgattcc atctgccaca gttttgctgc gatgccgacg ggtggatttt
     5581 cgacgaaaaa cgtcagtgtc ggatattacc agagcagtta tatcgattat gtcattctcc
     5641 tgttcatgtt cattgccggc gtgaacttct cgctgcatta taaactggtc agcgggcatc
     5701 cgaaacaatt tttccgggat ccggaatttc gcgtttacct ttacatcacc gtgattgcca
     5761 ttctcgtcgt gtctgccaat atttacggga cggtttatcc ccggatacag gatgctttac
     5821 gctatgcatc attccaggtt gtttccatca tgacaaccac cggatttgta accgcagact
     5881 ttgaacaatg gcctgtcttt tcgcaattga tactctttgt ctttatgttt ctgggaggga
     5941 tggccgggtc aaccgcgggc ggtatcaaga tcatgaggat catcctgctt gtcaagcatt
     6001 gttatctgga aatattcaga attattcatc ctcatgccac gacgatggtc aaactcggca
     6061 ggacgccggt ttcccagaca atcatgagaa gcatttgggg cttctttatt ctctatctgg
     6121 tcattttcat gatcggagct attgcactgg cggctctcgg cctggatatc atttcagcgc
     6181 tgaccgctat ggcaacctgt ttaggtaacg tcgggccggg gttgggcagt gtcggcccca
     6241 tggacaatta ttcggttatt cccgtgacag gccagtggat tctgattgtg tcgatgctgc
     6301 tgggacgtct ggaaatttac accattattg ttctgctgat gcctgccttc tggcgcaaat
     6361 aatgcccggc tgaaagcgtt cggttcgcga ggccgttgcg ttttgtttga aagataaaca
     6421 tctctatctg acggagccgc gccggagcaa tgtttcacgt ctttacaaga cgcggttatg
     6481 atcaatggtg gccatgcttg gctttggatt tcatttcttt gtattcttca tgcagcgcga
     6541 tgtgatccat gtcctccata tcttccgctc tcttgaccca gtcttcgaat ttaatatcca
     6601 caccgaaaat acccaccatc tcatcgtcag tattaaaaat aggggcggaa accgtgaagc
     6661 ataaaacacc ggtcattttg gaaatgaaga aatcggagac gtagacctcg ccggtgcgca
     6721 ggggattgat aaaccactgc ctgtcggact ggtcggttcc cacgccgtaa ttttcatatt
     6781 tggcgcgatc ggcgatatcg gtaatatttc gtgtcgtttt ccggccgttc atatccacaa
     6841 cgtaggcaaa ttgaacggaa ggatattcgt cgacgaattt ctgcatcaga ggttcctgtt
     6901 ttgccgtatc catggttttc atcaagggtt gttcaatgat gtgcgtaacg gcggcaacag
     6961 cggctttttc tgcaatgtat ttgatctttt ccagctcgga catgaagcgt tccggcaggt
     7021 aaagacggac tttcttttcc atttcttcag gcgatatgga agtaatgcgt ccggattcat
     7081 actgttccat aacccacttg tgaattttgg aaacaccggg atgcctcttg tccaccgttt
     7141 tattgccttc cagattaaaa cgctggttga tccagaacgc aataccggcg ttgccggatt
     7201 tgtccgtgat ggtcgtggtg atcggtcgtt ttaaaatttt tcgcgtatca aagatattgt
     7261 aaatttcttc gcatttgatg agaccgtcgg catggacacc ggcgctggtg acattgaagc
     7321 ccgacccgac aaacggataa ttatggggta tcttcacgcc gatttccttt tcgaaatagt
     7381 ccgcaatatc ggtgatgacg gtggtgtcga taccgtttct tcctttcagg gcgatatatt
     7441 ctataattaa cccttcaatc gggggattac cggttctttc gccaagaccc agcagcgtgg
     7501 aattggcggc gctgcaaccg aaaagccagg cggtggttgc attgatcatg gccttgtgaa
     7561 agtcgttgtg tccgtgccat tccagcagat ggccgggaac gccggcgtca tgaataaatg
     7621 catggacaag cttgtcgacg ctgcggggaa gggcggcgcc gggataggtg ataccgtaac
     7681 ccatcgtgtc gcagagcctg attttgatgt cgatgccgct ttcctcccga agcttcatca
     7741 actgctgggc aaaaggaatg caaaaaccgt aaatgtcggc gcgggtgaca tcttcaaagt
     7801 ggcaacgggg aataatgcca aggtccagaa tggttttgac aattcccaga taatcattca
     7861 tggcctggct tcgttttttg ttgagtttca gaaagatgtg gtaatccgag acggaagtca
     7921 gaatgcccgt ttccttcagg ccgaactgct tgaccagggg aacatcgtcc ttggatgccc
     7981 tgatccagct ggtgatttcc ggataacgca gattgagggc catgcatttt tccacggcat
     8041 ctttgtcctt cttgctgtag agaaaaaatt ccgattgacg gatgaccccg ttgggcccgc
     8101 ccagcctgct caataactgg aatatggagg caatttgttc ggcggtatag ggagggcggg
     8161 actgctgccc gtcgcggaag gtcgtatccg taatgaaaat ttcctgcgcc ggttccatgg
     8221 gaacggtttg atggtcgaaa acaattttac tgacttccga ataagggaat atctctttgt
     8281 acaaattggg ttggtccaca tcgaccaatg ccgctttttt gattttactt ttttgcgcag
     8341 ccgtcggttt gttctccttc tttattttag ccatgtatat ctccggtatt gaatgtgttc
     8401 attcctggtt ttgtaagtca gcgcggctca tacgaacggt acagacgcgc tgattctgcg
     8461 tatgggcatt ttgcttaaac gatttttatc gataactcca ttaattgttc aatgctgact
     8521 tttgacggcg catcggtcat caaacaggcg gctttctggg ttttcgggaa ggcaatgaca
     8581 tcgcggatcg attccgcccc ggtcatgatc atggtcagcc tgtccagacc gaacgccaga
     8641 ccgccgtgag gcggtgctcc gtattcgagg gcatccagca ggaaaccgaa tttcagccgg
     8701 gcgtcctcgt cgctgagttt cagtgcgctg aagacctgag attgaacatc tttgtggtgg
     8761 atacgaatgc tgcccccgcc gatttccgaa ccgttgagca caaggtcgta agccctggcg
     8821 cgaaccttcc ccggatcggc ggttaaaagc ggcagatcct ccagaaaggg cgacgtgaac
     8881 ggatgatgtg tggagacaaa acgtttttcc gtttccgaat attccatcag gggaaattcc
     8941 gtaatccagg tgaaggccag cgctttggga tcaatcagtc ccagtttttt gcccagatga
     9001 agccgcaaat tccccagggc atcggaagca actttgggtt tgtctgcgat gaagaagata
     9061 atatcgccct ccctggcgtc cagggcatta ccgatggcct gcacttcgtc gggcttaaga
     9121 tatttaaaaa caggcgatgt ccagtcggcg gcgttgatct tggcccaggc caaaccgttg
     9181 gcgccgtaaa cggccacaac atctttcaag ccgtcaaggt ctttccggga aagtacggag
     9241 gcctgtttcg ctttgatggc tttgacgacg ccgccggaag aagcgacttc cgcaaaaagc
     9301 ttaaagccgg aatctttgac gatggacgtc aaatccacaa tttccatgcc gaaccgcacg
     9361 tcgggattgt cttttccgta gcggctgacg gcagcggcat acgtcagacg gggcagggga
     9421 agggcgaggt cgatatccag gcagctcttg aaaatagctt tcatcagccc ttccatcatc
     9481 tgcatgatgt cgtcttcggt aatgaaagac atttccacgt caatctgcgt gaattccggc
     9541 tgccggtcgg cgcgcaaatc ctcatcccgg aaacatttga cgatctggta ataacggtcg
     9601 aaaccggaaa tcatcagcaa ctgcttgaag atttgcggcg actgcggcag cgcgtaaaac
     9661 atgcccttgt aaattcggct cggcaccaga taatcgcgcg ctccttccgg agtgcttttt
     9721 cccagaaacg gagtctccac ttcaataaaa ccgttttctt cgaaatagcg ccgcgccgcc
     9781 gccgccattc tgctgcgcag aaaaagattt ttttgtatcg ccggacggcg caaatccaga
     9841 taacggtatt tcaaacggat attttcagaa atttcttcat catcgaagga gaagggcggc
     9901 gttttggact cgttcatgat gtccaaatcg gagacgagga cttcgacttc cccgcttttc
     9961 agggacggat tttccatgcc gtccggtcgc ctgcgcacct tacctttgac ggccagaaca
    10021 aattcagaac ggattcgatg ggcttcctta tgggcttttg cgttatcggg attaaaaacc
    10081 acctgcacca tgccttcgcg atcccgtaaa tcgacgaaaa taacgccgcc gtgatcgcgc
    10141 cggcgatgcg tccagcccat caaaacgacg tcctggccga tcagtgaagc atcaagattt
    10201 ccgcagtagt gtgtccgttt atcagtcgtt aaaaattcag ccaaaacttt ttacctctcc
    10261 ttgatgattt ttattatgga ttcatgcagg ccatccatgg gaatatcctg ctgttgtttt
    10321 gtctgcatgt tgcgcaagac aacctgtttt tcttcaattt ccttgtcgcc gaaaatgaga
    10381 gcataggcgc tgttgagctt atccgctcgt ttcatctggg acttgagact cttgtccgag
    10441 taatccatct ccgcggataa gccgcaaact ctgagccggt tggtcaaacc gaaggcgata
    10501 tgctgtgcat tcgcgcccag cgccgcaatg aaaagatcgg ttgtataggt atccttccgg
    10561 ttttccggca ggcaggcgac cagacgctca aaaccgacgg caaagcctat tccggttacg
    10621 tccggccccc ccagaaagct gaccaggccg ttgtaacgcc ctccgccggc aacggcgttc
    10681 tgggcgccca ggatgccggt ggtgacttca aaggccgtcc gggtgtagta atccagcccg
    10741 cggaccattc tggcgttgat gacaaacggt gtctgcaaat cggcaagaaa cgattgaacc
    10801 tgtgcgaaat gctgcgtgca gtccgaacac aagaaatcaa tgatctgagg cgcaccggca
    10861 agggtttcgg cgcaggaggg aaccttgcag tcgaagaccc gcaggggatt ggtgtgaatg
    10921 cgccgctgac aatccgggca cagacggctt tgcctgtcct tcaggaaatc gatcaccgcc
    10981 ttggaaaatt cgggacggca ggcgctgcag cccagggaat tgatttccag ttgcagattg
    11041 cgcaaacccg cgctgttcag gaaatgcatc aacatgaaaa tcacttccgc gtcggtttgg
    11101 ggtttgtcat cgccgaaaac ttccacatcg atttgattaa actggcggaa tcgacctttt
    11161 tgcggccttt cgcggcggaa catcggaccg aatgtaaata actttgtaac gggttcgttt
    11221 gcggacatgt tgtgttcgat gtaagcccgg atgaccgacg cggtggcttc cggtcgcagg
    11281 gtgatctggt cattgtcccg gtcggtgaag gtgtacattt ccttttcgac aatatcggtg
    11341 gtctcgccga tgcttcgctt gaaaagttct gttttttcca gaatggggac gcgtatttcc
    11401 cggaaaccga aagaatcaaa aattgttcgg gccagggatt cgattctgca gaaagcaagc
    11461 gcatcgtccg gcaggatatc tttaaaacct cgaattgctg taattttttc cataatccca
    11521 accaaatttt taagggtgca ttactaacac attcatgtct tcttggcaac gagaaaatag
    11581 gttgaagcag gggaaggggc aatggatgca atgagaaaga aatcaacgct ttcgcaccgc
    11641 acaaacgatt gcatcctgga cgcatacatt tgtgtgacgg gaggcgcttc ttaactcaaa
    11701 acaaagtgct tttcgcggaa gagctttatg caggcatcca caacgttttc gtcataaagc
    11761 acgcctttgt ttttggtgat ttcttccaga gcaaaatcaa tgcccaggcc gggccggtaa
    11821 ggcctgtggg acgaaatggc ttcgacgacg tcggcaacgg ccagtattcg cgattccaga
    11881 agaatatcgt agccttgcag cttttgggga taaccggttc cattcattct ttcatggtgc
    11941 tgcaaaatgg catcggcaac aggccagggg aattcaatat ccttgagaat gttgtaaccg
    12001 gattgggaat gtgtttttat cagttcaaac tcaagattgg tgagcttggt cggtttactg
    12061 aggatctccg aaggcacgga taatttcccg atgtcgtgaa taattgcggc cgtctcgatg
    12121 aattccacaa cgtccatgca taatcccatt tcctcagcaa tggcccgggc cagaatcgac
    12181 acccgttttt gatgacccgt tgtatacgga tcccgggttt ccaccatgga ggcaatagcg
    12241 cttacggttg catcgagcgt tttcttgatt tttataaaac tttgggagcg ttcctcttcg
    12301 agttgtttgc tgaccgttat gtcctttaaa aaattcagcg cggcacgctt tttctcccat
    12361 tcgattacgg tggtgtttag ctccacccat ttcacggatt tgtcgcggca aacgatcctg
    12421 aaagagtaaa cagacggtat ggcatcgcct ttgattcttc tgtcgtgata acccctgacc
    12481 atgccgatat cgtccggatg gataagatcg ctgaaattca tggaggtcag ttcttccatg
    12541 ggatagcctg tattgtttat ggccacggta ttggcaaaaa ggattttcat gttctgcgtg
    12601 atcaaaatgg cttcattggc gttttccaca acgacgcgat agcgcttctc gctttcccgc
    12661 aggttttttt cggcctgctt gcgttcggtg atatcgttgt aaagggcttg aaattgcatt
    12721 tggccgttcc acaaaacctg cttgcggaaa acctccaggt ggcgaatggt cccgtccttg
    12781 cggacaatgc tgacttcata attcggcgga acatattcgc cgtgttttct catttcaatc
    12841 cgtttttcgt attcgacaaa ggtttccggc gtatatctct gcagatggga aatggatttc
    12901 aactcatcga tgctatcgta gccgaacatg tttaaaattt cccggttggc atacaataac
    12961 tcaccgtcgg acgtgacaat acgtacgccc agcggagatt catcgagcga attgcggaaa
    13021 ttattttcgc ttcttttgag catgtcttcc atgctttttt tatcccgcct gatttttgct
    13081 tcggcgattt cccgtttcac ggcgacagaa agcttggcga gcttgtcttt catgacatag
    13141 tcgtgagcgc cggccttcat gacctcgacg gccgtttcct cgccgatggc gccggaaacg
    13201 acaataaagg gaatattcgg attgatttcc tgagccagtt tcagggcggc cggcgcactg
    13261 aagccgggca tcttgtaatc gcacaggatc acgtcccatg tttttttctc cagagcgttt
    13321 ttcatagcat catgcgtatc cactcgttcc cattcaggct gataacctcc gctgcgcagt
    13381 ttccgtaaaa ccagattggt atcgtcatcg gaatcatcca caatcaggac gcgaagcact
    13441 tcattcatat tcgtttctcc cttaatttat gcaatgcaat catcttttgt cttccgtcat
    13501 ccaacggcct tcttccaaat gtgttttatg caggcggggg atagcccagc gtaaaataaa
    13561 aagtggcgcc ttgatgggga acgctttccg cccagacggt tcctccgtga cggctgatga
    13621 ttcgcctgac cgtggccagc ccgattcccg ttccgggaaa ttcttcgatg ttgtgcaagc
    13681 gttggaacgg agcgaaaatt ttatccacat actccatgtc gaaaccggcg ccgttatccc
    13741 gaacgaaata tacttttttc ccgttctgct gcgttgagcc gaattcaatt tcagcgtccg
    13801 tcttcttgcc cgtaaatttc caggcgttgc ccaggagatt ttcaagtaca atttttatta
    13861 atctcgaatc ggcgtaatcg gtgagggaat ccggcatggt cacctttacg ggacggtcgg
    13921 gatgggcttt gcgaagttca tcaacgaccg tcaacgcaat attggagagg ttgatatcgg
    13981 ctttatcaat ttccgtgcgc gttattctgg agagtttcag catgtcgtcg atcagctcgc
    14041 tcatgatttc ggtcgctctc ctgattcggc tgatataatc tttggcctga ttatccagct
    14101 tatcgctgta gtcctcaacc agcgcaaggc tgaatccctc gattgttctt aacggcgccc
    14161 gcaggtcatg cgatacggaa taagaaaacg cctccagctc tctggtgcgc tcctgaacct
    14221 tttcttccag ggattcgttc agcctgacaa tttccatctc tgcttttttg cgttcgctga
    14281 tatctaagcc aatgccgaga agataatcct ggcccgagac ggtaagtttg acgccggtaa
    14341 aaaacataaa aagatgatcc ccgtttttag tgatcatacg tgcctcggcg gaagaatatc
    14401 ccttggtcat aacgtcggat atgccttttt taatgtttga tgtatcgggt tccaggccgc
    14461 cgaaccaatc cataacatat tttccctgta gttcatctcc ggaatagcct gttaacactt
    14521 caaactgcct gttccactgc accagatgtc cggtgtcgtt atagagataa agaatgccgg
    14581 gaacgctttc gagaatggct ttgttcatta acagggtccg ttttaattct tcttcggcct
    14641 gtttgaggga ggtgatatct tccagcatgc cgagttggca ataagctttg ccctcggcat
    14701 caggaacaaa gaaaccgcga tccctgatcc atacataatg cccttttctg tgtttcatgc
    14761 ggtattgcga ctcccagaag ccgcaggctt tttcggctgc ctccagagat tgaatcgttg
    14821 catccctgtc gtcaggatgc aggaggattt cccattgtcc gaagccgccg ctgatttctc
    14881 ccatatcgta tccgaggacg tttgcgatac tggaccccca gataatccgg ccggtggata
    14941 aagtatattc ataagccact tgtccggagg ccgccacaat cagttcatac cgccgcatcc
    15001 acgtttgcag ggattcctcc attttcttgc gttctgttac atccatcccg aatgctaaaa
    15061 tgccggcaaa cctgcctttt tcaataatcg tgctgtttct ccacgaaatc agacgttctt
    15121 cacccgattt tgtcacgatg ggattttcca tggattccgg caaatcgttg ctgaatttcc
    15181 attttttgaa tgcctcccag acatagggat aacggttttt cggaacgatc agatcaaacc
    15241 agtttaaacc ggcaatgtct ccggcccggt aaccggtaat cttttctccg gcttcgttca
    15301 atgaaacgac gttgccttcg gtatcgagaa ccacaatcaa tgcattggcc gttctgagca
    15361 gtttttcagc gtaatttctc gactccagta actcctgttg aattcttttt cgctcggtga
    15421 tgtcatgaag gctgacggta ataaattcga taacatcatt gttgacgaca ttacttcccg
    15481 atatttcaaa ggtgagccag gttccgtctt tatggcggat gcgagtttcc gttttttgaa
    15541 cgggcacatt tttatccgta aacagaatct ggaagcatct cattaaatcc tccagatcat
    15601 cagggtgtac gttaagaaac gtatcctggc cgattcgctc ctcctgtttg taccccagga
    15661 gtttttccac ggcgggattt tcataaagca ttgtgccgtt tttatccacc agcgcaataa
    15721 tgtccgagga ttgctcggca aatgaacgga aacgcttttc ttcccggagc agtttttctt
    15781 ctgcaacttt tctttcggtg atgtcggtac ccacacccag gatatgatgt ttaccgccga
    15841 tatcgaatct gacccctgtg taaagcatgg gaatcaatcg tccgtctttc gtttgaaggt
    15901 tggcctccac gttggaagcc gatccacggg tcatgatggt atcgatgccc gccttgatgt
    15961 ttaataaatc gttttgccgg tccttaaacc agtccagaac aaaactgtct tggatttcat
    16021 gggcgtcata gccggtcagg atttcgaatt gtttgttcca gtgtaccaga cgtccgtttt
    16081 catcgatcag ataaagactc cccggaatgc ttttgagaat ggcttcgtta taggcttgag
    16141 cctgttttaa ttctctttcc gcctgtttgc gttcggttac gtcgtgaata atagaatgca
    16201 aaacgtaaga gccattcctt ctgaccctgc tggaatatac ctcgacgtct ctgatggacc
    16261 cgtcggcgcg gcgatgacgg aattcaaaat ggaccttatg ttcctgcata accttatcca
    16321 tctcgtgctt gatttcgtca ggggaaagaa tattgatttg cgagattttc atctgcttca
    16381 tttcttcccg cgtccagccg taatagttgg ctgcagcgaa atttgcatcc agaatcgctc
    16441 ctgtttcggg atccataatg agtttgaccg ccgaatggtc ttcaaagagt ctgcgataat
    16501 tatcctgcgt tgccttgaga ctttcctgaa tgtttttttg atcggtgata tccgcattga
    16561 acccgtgcca gacaatggtg ccgtcgggca gcttttcagg tgttgattgg cccagcatcc
    16621 atttaacagt gccgtcgggc aactgcaccc ggtattcgtg cttccagtcg gtcatatgtt
    16681 gagtggaata ttcgatggag tttaaaaaat cgtcaacgtc ttccggatgt acggcatgta
    16741 agatcggaga ataatcatga gcgacatctt ccggaaaaca gccgaaaata gttctaattg
    16801 catcggtgga aaacggcagg caatacgtcc cgtccggttt ttttacaaac tggtagatca
    16861 ttccgggcac atgggcggat aattttttga ataaaagatc gcgctgtttc agggcctctt
    16921 ccgcctgctt gcgctcggaa atatcgcgga tgatcatact cgtcaagggg gtgcctttgc
    16981 gatccgtgaa aatcacggag gaaatttctc cctcgaattt cgatccgtct ttgcgcacaa
    17041 aggtcaattc acccctaaac cggccggttt gcttgcgttc ttcaagagcg gccggcaaac
    17101 gcggatcact cagatccata acgccgggtc tgccggctcg gcggatatct tcttcggtgc
    17161 ggccgaacat tttgcaggca gcggcattgg ccgaaaggac atctccgtcg gggatggtca
    17221 gcaaaatggc atccgtgctg ttgtcgacaa tggaatggta tttcttttcg ctttctatta
    17281 actcatcctc catgcgtttg cgttgcgtga tgtctctcga caaaccatga agaccggtca
    17341 gtttcccctg ctcatttcga agcccgctga cggttgtttc cagccagcgg gtcgagccgt
    17401 ttttgtgata atattcaaga gtcagcgtga cgtttttttc cggattactt ttcccctgtt
    17461 tttcatcaga aagttcacga tccatgatgt ttacggctgc cgctaaggat tcaggcgtca
    17521 gttgctcttc aaccgtctgg cgcaatcttt ctttcggggt gaagccgagc actttcttga
    17581 cggacgggct gacatacgtt gttctcatgc tcatatccat cgtccagaca atgtcgctca
    17641 ttttttccgt gagcattctg tacagacttt cgctttcgcg caatgcagct tctgcctgtt
    17701 ttcgctcgga gatgtcttca atatggacca ttaccagatc ggggggcaca aaactgtaag
    17761 aggccttgat ataggttccg ggaataaagc gttccgagaa taattctttt ttgatggttg
    17821 tttttttcgc aaagcagtcc tgtatgtcct tta
//
LOCUS       DAONQZ010000046        17667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3615_length_17667_cov_18.570974, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000046 DAONQZ010000000
VERSION     DAONQZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17667
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3615_length_17667_cov_18.570974"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="asd"
                     /locus_tag="PLR13_08100"
     CDS             complement(<1..1022)
                     /gene="asd"
                     /locus_tag="PLR13_08100"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQC19338.1"
                     /translation="MLKIAIVGATGIVGQQAIVSLLGHPWFKITKLVASERSAGKKYI
                     DALRDANGSIRWWCPEELPGHIANMVVEEAARFDPKSVDIIFSTMDAGPARDLEPKYA
                     KTTPVISTASAFRYEPDTPILVGGVNMDHAALLAVQQKNRGWKGFISPKSNCTIVGLV
                     VSLKPILDNFGVKQVVATSLQAMSGAGRTPGLSAMDMTENVVPYIPGEEPKVETEPLK
                     ILGKLTGKIIVNAAFGITATCTRVPVLDGHMVCAFVQTKKKCSPEDVKKAMMNYGKDF
                     AELHLPSSPAHLIDVTDDPFRPQPRMDRDKGGGMTVTVGRIRKDPVIENGIKYVCLAH
                     NTKIGAA"
     gene            complement(1039..1989)
                     /locus_tag="PLR13_08105"
     CDS             complement(1039..1989)
                     /locus_tag="PLR13_08105"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQC19339.1"
                     /translation="MIIFRSTENITGDFRGSFVSIGNFDGVHLSHQFICRKLASEAKQ
                     AGTKSLVITFDPHPKMILHPNIRPFFLITTLDEKLERLAACGIDGVLVIPFTMEYSKT
                     TAEEFVHNFLGRKLAIGKIIIGHDYTFGQGKKGNSDYLIAAGRDMGFAVEVVDAVKSG
                     NDIISSTLIRKSIIEGNLKKAAVLLGRYYNVAGKVVTGFGRGVTLGFPTANIEPEKEL
                     LPPPGIYAAFVDAEEKRYQAVLNIGAKPTFEDYTFSFEVHLLDFSGDLRGKRINTDFV
                     EKLRDIVKFDSPETLKKQIAADVEKAKTILLENISNRQGT"
     gene            2106..2192
                     /locus_tag="PLR13_08110"
     tRNA            2106..2192
                     /locus_tag="PLR13_08110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2140..2142,aa:Leu,seq:cag)
     gene            2311..3027
                     /locus_tag="PLR13_08115"
     CDS             2311..3027
                     /locus_tag="PLR13_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQC19340.1"
                     /translation="MIEPLVNNLSFYLESSLMLSVLAAYLGGVIISFTPCTYPLIPVT
                     VGLIGIQGPSSKIRGFFLSLFYVAGMAVTYAVLGAAAALSGKIFGQMQTTPWTYFIMA
                     NLCLVMGLSMLDVFNLSIPVPQKLMQWTGANGKKGFLNSFLIGAVSGVVIGPCTAPAL
                     GVLLGYVAVKTNVVLGMSLLFIFAFGMGTLLIIAGTFAGMIAALPRSGAWMEKIKFIS
                     GLILIGAAEYFLYIAGRLSI"
     gene            3037..3546
                     /locus_tag="PLR13_08120"
     CDS             3037..3546
                     /locus_tag="PLR13_08120"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQC19341.1"
                     /translation="MMNKKILLAIALVFVFALVAFSFEQFKPSVFTRRDKVSAPDFSL
                     KDIHGKTFRLSAQRGNPVIMFFGTTWCPACREEMPLYRNLYNKYAGRGLKFLYIDINE
                     SAERVARFAGQNAFPYHVLVDGDGSVAGDYNIIGVPTLILLDREGNIVDVGHRISDLP
                     VDRLFPVKK"
     gene            3564..3782
                     /locus_tag="PLR13_08125"
     CDS             3564..3782
                     /locus_tag="PLR13_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQC19342.1"
                     /translation="MKMNERNWLQNLTRSNTDKWIGGVCGGLGESSPVPAWAWRLLFV
                     TLVLCFGTGVLLYILLWIFVPDKSTIQG"
     assembly_gap    3857..3956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3957..4605
                     /locus_tag="PLR13_08130"
     CDS             <3957..4605
                     /locus_tag="PLR13_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19343.1"
                     /translation="IYLYEWTNPYENNCNSFYIGGGVNALIDPGLTGYVPDLLDRMSA
                     DGIQKEEIRYVINTHSHPDHFQGSELFDQEKVKVGLHTREIEFLKGDGGELYSLFGIN
                     APRMHVNLPLEEGDLALGDQIFKVILAPGHSPGSIGLYWPDQKALFCGDVIFDQSVGR
                     TDFPGGNGDLLKKSILAFAKLDLEWLLPGHMGILHGKGSIQNNFNLIIKGIFPYI"
     gene            4634..6319
                     /gene="ettA"
                     /locus_tag="PLR13_08135"
     CDS             4634..6319
                     /gene="ettA"
                     /locus_tag="PLR13_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQC19344.1"
                     /translation="MANEGNKVIYSMIGVSKIYDKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDQEFLGKTILSPGYTVGYLEQEPHLDNDKTVRQIVEQGVQSTMD
                     LIQEYNDINEKFAEPMSDDEMAKLCERQGAVQEKLDALDAWDIDSRLEMAMDALRCPP
                     GDTSVKVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTII
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKNEEKAESDRIKTLER
                     ELEWIRMSPKGRHAKSKARISAYEELLGKTMEKESGTREIFIAPGPRLGDLVIEAKDV
                     NKGYGDKLLVEGMTFSLPPGGIIGVIGPNGAGKTALFRMITGREKPDAGTIRIGDTVK
                     LAYVDQSRDSLDPNKNIWEMISDGQDIIAIGNRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALENFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDYRKRYGNAASQPHRIKYRQLTRR"
     gene            6644..7963
                     /locus_tag="PLR13_08140"
     CDS             6644..7963
                     /locus_tag="PLR13_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQC19345.1"
                     /translation="MNKKRLVLLVILFITIGLFSLQQPSENSFRGDAVVSEPTAPEKG
                     TRPLKSISDKFEKGETLSSIFKKYGMHTDDLFELKSAVASIQSLRDIKPGKPYSFTLN
                     KDNYISSFTCGIDDDTILKIERVDGCFQAQKYNIPYETRILTIGGTIQNTLVSAFGNE
                     REDYLLALQAADILAWDIDFNTDVRKGDTFRIIVEGLYSEGKFKKYGRILSIEFVNGS
                     QTYNAYLFEQDGKTDYYNADGQSLRKAFLKAPLSFRRISSHFSRSRLHPILKVRRPHH
                     GIDYVAATGTPVSTVADGRITFAGYKSGYGKIVIVNHANGYQTYYGHLSRIKSNIRPG
                     KKVQQGDLIGNVGATGLATGPHLHFEMRQGGRAINPLAVKKVAGSPVPAAQMAEFKNT
                     TQTMNNIYTVALFFDTNNTQRKNHSLQPALLYASLASSSPQGKFRKN"
     gene            complement(8014..8349)
                     /locus_tag="PLR13_08145"
     CDS             complement(8014..8349)
                     /locus_tag="PLR13_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HQC19346.1"
                     /translation="MPFKTLITVLICNFIFVMIAVPLALRKVPKNVIYGFRIKATLEN
                     DFVWYEANAYFGKVFIIGNMVCALLILILYFSDVVPAQNFVNIGMAILIIPALVAALL
                     TFRTIKTKH"
     gene            8461..10227
                     /locus_tag="PLR13_08150"
     CDS             8461..10227
                     /locus_tag="PLR13_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQC19347.1"
                     /translation="MNNTKIPTVKPFVHHLRPRGLSRRIPLIETLRNYRREWLMHDIL
                     AGLVLTAILVPVGMAYASVSGLPAIYGLYATIIPLIAYAIFGPSRILILGPDSALAGL
                     IAAAILPLAAGNTDKAIALAGLLAILAGAFCILAGLVKFGFITDLLSKPVRIGYLNGI
                     ALTVLIGQLPKVLGFPVGGNNILQEVNSLIQGIAGGQINWMSCLIGCSCLMIILGCRQ
                     WFSRIPGVFIAVAGATIISSWFHLSASYGIAVVGPLPQGLPQFQIPSVSFAEFKALFA
                     AAFAIALVSFADMSTLSRTFALRAGQEVDSNQEIIALGAANMAAGLFQGFPVTSSASR
                     TPVAESAGAKTQVTGVVGAVCIALLLIFAPNLLKNLPQAVLGAVVICACISIVEVRAL
                     ARLYKLRREEFVFSIACFLGVVSLGIIKGIFIAIGLALFSFIWRAWHPYDAVLGHVEG
                     LKSYHDISRHPDARLIPGLVLFRWDAPLFFANAEAFRENVLHAIAEAPTPTKWVVVTA
                     EPITDVDTTAADMLAELDNVLHESGMALFFAEMKDPVKDMLKRYGLFTRFGEENFFPT
                     IEQAVERYLTLHKIDWPESHKR"
     gene            complement(10491..10811)
                     /locus_tag="PLR13_08155"
     CDS             complement(10491..10811)
                     /locus_tag="PLR13_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19348.1"
                     /translation="MSSELLRYEINRKIRQVLVSHNADMTKISYSFVHRTVYMSGNLV
                     RESQGEFSLPVIEGMIRELMKLPRVQKILFDLENWIISNEPGALNIVKKKGLGQHPAI
                     KDSV"
     gene            complement(10844..11143)
                     /gene="yhbY"
                     /locus_tag="PLR13_08160"
     CDS             complement(10844..11143)
                     /gene="yhbY"
                     /locus_tag="PLR13_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HQC19349.1"
                     /translation="MEKLKGSQKKYLRAQAHHLKPVVMIGAKGSTTQLISSVNAALND
                     HELIKIKFGEFKEAKKEISQEIAGKTKSELVGLIGNIAIFYRQNPNPERRKIKLS"
     assembly_gap    11289..11388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11470..12432
                     /gene="trxB"
                     /locus_tag="PLR13_08165"
     CDS             11470..12432
                     /gene="trxB"
                     /locus_tag="PLR13_08165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQC19350.1"
                     /translation="MSKANLPQYDCDTIIIGGGPGGYTAGIYAARSGLKSLLVEGAST
                     VSQITITDLIENYPGIPDGINGFDLMQLFKKQAQNFGLEIIANDVHSIKKAGDNPAIW
                     EIAVGDKSYRTLSVIAATGAQWNKLGVPGEEEFAGKGVSYCATCDGPFYRNRDVVVVG
                     GGDTAIQEALFLTHFAKKVSVIHRRDRLRAAAILQKRAFAEKKIEFVWKAKLAEIAGQ
                     EFVTGVRVADVESGKINHIEAEGVFIFVGLTPNTALFRDVLNLDKGGYIITDDNMRTS
                     APGIFAAGDCRAKQFRQVVTAAGDGANAIYSAELYVDELKGQTY"
     gene            12562..13395
                     /gene="lgt"
                     /locus_tag="PLR13_08170"
     CDS             12562..13395
                     /gene="lgt"
                     /locus_tag="PLR13_08170"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQC19351.1"
                     /translation="MEAFINTWQQLPSHINPALFTIGSFQLRYYSLMYLVGFAIVYLL
                     TTYRISNEHYEYKKEMLQDYLIWGMFAMIVGARLGEVLIYNFSYFSQHPLEIFLPFSF
                     SNGIHFTGISGMSFHGGVVGVMIYSIYFLRKRQINFWRFADLIVPSIPLGYTFGRLGN
                     FLNGELWGRVTTMPWGMYFPFDPTQHLRHPSQLYEAFFEGIFLFVILWLLRKKKFADG
                     FLLGLYIFGYGFVRFMIEFFREPDFMVGSVSIGQVLCVLMMAAGLIIVLWRRQIASPA
                     R"
     gene            13409..14740
                     /locus_tag="PLR13_08175"
     CDS             13409..14740
                     /locus_tag="PLR13_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC19352.1"
                     /translation="MGKHLIIVGGGAGGASAAAEAKRNDPELNVAILQSGKFIAYASC
                     PTPYYIGDIIKDENRLVARTPEEFRKRGVEVQLDTEVVNINCNANVVETRDNLMLPYD
                     YLVIGTGTNPFVPDIPGRELPGVYVLNNLENAIHIKAWLRERKAKRVVIIGGGFIGLE
                     MSEALHEVGISTTIIHKHSLPANRWSKELSSVMLEELQSHGVEFIGNAQAKAIEKSSG
                     DSLKVITDAGEWQGDMVLLAVGVRPNAALAKSAGLTIGKTGAIAVNFAQQTSVENIYA
                     VGDCCESWNRVSRRWVNIPLGDIANKQGRVAGRNIGAKPLTFDGIVGAQSFRLFDLEC
                     AATGLSEKEALAAGFVPISNITWGSAMAPAMGMKKIGLKLTADKSSGKLLGAQAVGVA
                     GAVGRINALSVALWTGLNIDEVGYLDLAYAPPFSAAWDIIHNAAQALRRMI"
     gene            14759..15697
                     /locus_tag="PLR13_08180"
     CDS             14759..15697
                     /locus_tag="PLR13_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQC19353.1"
                     /translation="MTLWWTIFGVFILAMLALDLGVFNRKAHVIKMKEALLWVSFWVS
                     LAVAFGVGVYYFYGHSKALEFFTAYIIEYSLSIDNLFVFLLIFRFFNVPQAYEHKALF
                     WGILLALITRAAFIFAGVALINKFHWIMYLFGAFLIYTGIKLAMNKQTEVHPDKNIAI
                     KMLRYVVPVTKEFHEARFFIIKNGKRFATPMLAVLLALETTDILFAVDSIPAVLAISR
                     DPFIVYTSNVFAILGLRSLFFAISGLMKLFHLLHYGLSAILSFVGVKMLIADFYHIPV
                     GISLTVISSILVISIVASIIWPDTKYEDIPNITMKD"
     gene            15702..>16177
                     /locus_tag="PLR13_08185"
     CDS             15702..>16177
                     /locus_tag="PLR13_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HQC19354.1"
                     /translation="MQNGFLTPSQIQLKKWARLDDAKARHEEGVFLAEGVKVVEELLK
                     SDWQIKALLVMPEKIKFWKKLDLSREEEIPVYQLKRTQWEKIGQDREPEGIMALVEMK
                     QAPDFSSWLAQTSGNVLILHEINNPLNLGALARSAQWFGFNSIILSANSVDFTNPKA"
     assembly_gap    16178..16277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16278..17104)
                     /locus_tag="PLR13_08190"
     CDS             complement(<16278..17104)
                     /locus_tag="PLR13_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQC19355.1"
                     /translation="MKSKIKILVFLCLAISVMFSLVNCRQQNGVCDCPPSKFENGIYY
                     SQTYEDYILAHVFQDVADGFYIDVGANDPNIFNVTRFFYERGWHGINIEPNAELYLQL
                     RSFRQRDINYNVGIATTEGRMIFYQQAYQSDALMPLSTFDKEIVDIQKKNGILFKEIV
                     VPVTTLNNLLGKISVREITFITIDVEGFEKQVLESIDLARYKPVVLCIEASRPGTGDL
                     SYMLWEQIVLNNNYVFAMSDGVNRYYLHKSHIELLPRFIDIDRCVKLSKYKRQVKMDG
                     "
BASE COUNT         4247 a         4259 c         4308 g         4553 t
ORIGIN      
        1 gccgcgccga ttttggtatt gtgcgccaga cagacgtatt tgatgccgtt ttcaataacc
       61 ggatccttgc ggatgcgtcc gacggttacg gtcatgccgc cgcccttgtc tctatccatc
      121 cgcggttgcg gacggaacgg atcatcggtc acatcgatca gatgcgccgg tgaactgggc
      181 agatgcagtt cggcaaagtc cttgccgtaa ttcatcatgg ccttcttgac gtcttccggc
      241 gagcattttt tcttggtttg aacaaacgcg caaaccatat ggccgtccag taccggtacg
      301 cgggtacagg ttgcggtaat gccgaacgcg gcgttgacga tgatttttcc ggtcagcttg
      361 cccagaattt ttagcggttc ggtttccacc ttgggttcct cgccgggaat ataaggcacg
      421 acgttttcgg tcatatccat cgccgacagg ccgggtgttc gtcccgcgcc ggacatggcc
      481 tgcagcgaag tcgccaccac ttgttttacg ccgaagttat ccagaatcgg cttcaacgaa
      541 acaaccagtc cgacaatggt gcaattggat ttgggtgaaa tgaaaccttt ccagccgcgg
      601 tttttctgct gaacggccag cagggccgcg tgatccatat tcacaccgcc caccagaatg
      661 ggggtatccg gttcataccg gaatgccgac gcggtgctga tcaccggagt ggttttggcg
      721 tacttgggtt ccagatccct ggccggtccc gcgtccatgg ttgagaaaat aatatccacg
      781 gatttcggat cgaaacgggc tgcttcttcc accaccatat tggcaatatg ccccggcaat
      841 tcctccgggc accaccagcg gatcgaaccg ttggcgtccc gtaacgcatc aatatatttc
      901 ttgccggccg atcgctccga agccaccagt ttggtgattt taaaccaggg atgtccgagc
      961 agactgacaa tcgcctgctg gccgacgatt ccggttgcgc cgacaatagc aatttttaac
     1021 ataattcaaa aacctcctct aagttccttg acggttgctt atattttcaa gcaaaatcgt
     1081 tttcgctttt tccacatccg cggcaatctg tttcttcaac gtttcgggag agtcaaattt
     1141 gacaatgtcc ctcagcttct ccacaaaatc ggtattgatc ctttttcccc tcagatcacc
     1201 cgaaaaatca agcaggtgta cttcaaagga aaaggtataa tcttcaaacg tcggtttcgc
     1261 tccgatattt aaaacggctt ggtaccgttt ttcttccgca tcaacaaacg ccgcataaat
     1321 gcccggcggc ggcagcaatt ctttttccgg ttcgatgttg gccgtcggaa atccgagcgt
     1381 gacgccccgt ccgaaaccgg tgaccacctt tcctgcgaca ttgtagtatc gtcccagcaa
     1441 gacggcggct tttttcagat tgccttcgat aatggatttc ctgatcagcg tgctgctgat
     1501 gatatcgttt cccgatttaa cggcgtcaac gacttcaacc gcaaacccca tgtcccgtcc
     1561 cgcagcaatc agataatcac tgttgccttt tttcccctga ccgaaggtgt aatcatgtcc
     1621 gataataatt tttccgatgg ccagtttccg ccccagaaaa ttatgaacga attcttccgc
     1681 cgtcgttttg gaatactcca tggtaaaagg aatgaccaga acgccgtcga ttccgcaagc
     1741 cgcaagccgc tccagttttt cgtcaagcgt cgtgatcaga aaaaacggcc tgatattcgg
     1801 atgaagaatc atcttgggat gcggatcaaa cgtaataacc agtgatttgg tcccggcctg
     1861 tttcgcttcg gaggcgagtt tccggcagat aaactgatgg cttaaatgca cgccgtcgaa
     1921 attgcctatg gagacaaatg atccgcgaaa atctccggtt atgttttccg tactcctgaa
     1981 tataatcatt gtttcatact ataaacgtca accagtagct tgataacata acatgttgga
     2041 cattaaattc aagctcattt tatcttgaca agaacgtgcc gatcggtata gtgcactttt
     2101 tgtgtgccga agtggcggaa ttggtagacg cgctaggttc agggtctagt aggcgtgcgc
     2161 ctgtgctggt tcaaatccag tcttcggcac cattttttat cattattgat ttgcataatc
     2221 ataacttgtc acagccgaaa acgatctttt tctgttgatt ttttaaactt ttttatccgg
     2281 taacgaacaa ccagacacag gcagtatttg atgattgaac cccttgttaa taatctgtca
     2341 ttttatcttg aaagctcgct gatgttatct gtccttgccg cttatctggg gggcgtgatt
     2401 atcagtttta cgccctgcac gtatccgctg attccggtaa ccgtggggtt gatcggcatc
     2461 cagggaccgt catccaaaat ccgcggtttt ttcctctcgt tgttttatgt ggccggaatg
     2521 gcggttacct atgcggttct cggtgctgcg gcggcgctgt cgggaaagat attcggtcag
     2581 atgcagacga cgccctggac gtattttatc atggccaatt tatgtctggt catgggattg
     2641 tccatgctgg acgtgttcaa cctctccatc cctgttccgc aaaagctgat gcaatggacc
     2701 ggtgcaaacg gcaaaaaagg atttctgaac agttttttaa tcggcgccgt ttccggcgtg
     2761 gtcatcggac cctgcaccgc cccggccctc ggggttttac tcggttacgt ggcggtcaaa
     2821 acaaatgtgg ttctggggat gagcctgctg tttattttcg ctttcgggat gggaacgctg
     2881 ctgattatcg ccggcacgtt tgccgggatg attgccgctt tgccgcgatc gggagcatgg
     2941 atggaaaaaa ttaaattcat atccgggctg atattaattg gcgctgcgga atatttcctg
     3001 tatattgccg gaagactgtc tatataaaaa gaggtgatga tgaacaaaaa aattttactg
     3061 gcaatcgctc tggtgtttgt ttttgcgttg gttgctttct cttttgaaca gtttaagccg
     3121 agtgttttta caagacgcga caaagtttcg gctcctgatt tttcattgaa agacatccat
     3181 gggaaaacat tccggttgag cgcccagcgg ggaaatccgg tcattatgtt tttcggcacg
     3241 acttggtgtc ccgcctgccg cgaggaaatg ccgctttaca ggaaccttta caataaatat
     3301 gccggacgcg gattaaagtt cctgtatatt gatatcaacg aatccgccga acgggtggca
     3361 cggtttgccg gacaaaatgc ttttccgtac cacgtgctgg tggacggaga cggcagtgta
     3421 gccggcgatt ataatattat cggcgttccg accctgattc ttctggacag agagggcaat
     3481 atcgtcgatg tcggtcaccg aatatctgac ttgccggtgg acaggctttt tccggtaaaa
     3541 aaataaacag aattccgggg ggaatgaaaa tgaacgagcg caactggctg cagaatctca
     3601 caagatcaaa caccgataaa tggatcggcg gggtgtgcgg cggtctgggg gaaagttctc
     3661 ccgttccggc gtgggcgtgg cgtctgttgt ttgtgacgct tgtgttatgt ttcggcacag
     3721 gggttctatt gtatatcctg ctgtggattt ttgttcccga caaatcaacg atacaggggt
     3781 aagcatgaga ttaaaaaacg atatttatct ttacgagtgg accaatcctt atgaaaacaa
     3841 ctgcaacagt ttttacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatt
     3961 tatctttacg agtggaccaa tccttatgaa aacaactgca acagttttta catcggcggc
     4021 ggcgtcaatg cgctgatcga ccctgggctg accggttatg tccccgattt gctcgaccgg
     4081 atgtccgctg acgggattca aaaagaagaa atccgttacg tcatcaatac ccattcgcat
     4141 cccgaccatt ttcagggatc ggaactgttc gatcaggaaa aagtcaaggt cggtttgcac
     4201 accagggaaa ttgaattttt aaaaggggac ggaggagaac tctacagcct cttcgggatc
     4261 aacgctcccc ggatgcacgt caatttaccg ctggaggaag gcgatctggc gctgggcgat
     4321 cagattttca aggttattct tgcccccggt cattctcccg gttccatcgg tctatactgg
     4381 cccgaccaga aagcgctgtt ctgcggcgat gtgatcttcg atcaaagcgt ggggcgcacg
     4441 gattttcccg gcggcaacgg cgatcttttg aaaaaaagca ttctcgcctt cgccaagctg
     4501 gatctggaat ggctcctgcc cggccacatg ggcattctgc acgggaaagg cagcattcag
     4561 aacaatttca atctgattat caagggcatc ttcccttaca tataaatcct ctatcgcgga
     4621 gaacgacctt tttatggcca acgaaggaaa caaagtcatt tactccatga tcggggtaag
     4681 caagatctac gataaaaaac cggtgctcaa ggacatctac ctgtcttact tttacggggc
     4741 gaagatcggc gtcatcggtt tgaacggctc ggggaaaagc tctcttctga aaattctggc
     4801 cggtgtggac caggagtttt tgggaaaaac cattctttca cccggctaca cggtgggtta
     4861 tctggagcag gaaccgcatc tggacaacga taaaaccgtg cggcagattg tggaacaggg
     4921 cgtgcaaagt acgatggatc tgattcagga atacaacgac atcaacgaaa aatttgccga
     4981 accgatgtcc gatgacgaga tggccaaact ttgcgaaaga cagggcgccg tgcaggaaaa
     5041 actcgatgcc ctggatgcct gggacatcga ttcccgcctg gaaatggcga tggatgccct
     5101 tcgctgcccg cccggcgaca catccgtgaa agtgctctcc ggcggtgaaa aacgccgcgt
     5161 cgcgctgtgc cgcctgcttt tgcagaaacc ggacattctg ctcttggatg aaccgaccaa
     5221 tcatctggat gccgaatccg tcgcctggct ggaacatcat ctgcaaaaat acgaaggcac
     5281 gatcattgcc gttacccacg accgttattt tctggataat gtggccggct ggattctgga
     5341 acttgaccgc ggtcagggca tcccctggca gggcaattat tcctcctggc tggaacagaa
     5401 acagaaccgt ctgaaaaatg aagaaaaggc tgaaagtgac agaatcaaaa ctctcgagcg
     5461 cgaactggaa tggattcgca tgtcgccaaa aggaagacac gccaaatcca aggctcgtat
     5521 cagcgcttac gaagaactgc tgggcaaaac catggaaaaa gaatccggca cgcgcgaaat
     5581 cttcatcgcg cccggaccgc gtctgggaga tctggtgatt gaagccaagg atgtgaacaa
     5641 gggctacggc gataaactgc tggtggaggg catgacgttt tcattgccgc ccggaggcat
     5701 tatcggtgtc attggcccca acggggcagg caaaacagct cttttcagaa tgatcaccgg
     5761 ccgggaaaag ccggatgccg gcacgattcg catcggcgat acggtgaaac tggcctatgt
     5821 cgaccagagc cgcgattcct tagatcccaa taaaaatatc tgggaaatga tttccgacgg
     5881 acaggatatt atcgctatcg gtaatcgtca gatcaattcc cgcgcctatg tttcacgctt
     5941 taatttctcc ggaacggatc agcagaaaaa agtgggaacg ctctccggcg gcgagcgcaa
     6001 ccgcgtgcac ctggcccgca tgctcaagga aggcggcaat gtcctgttgt tggacgaacc
     6061 gaccaacgat ctggatgtca ataccttgcg cgcgctggaa gacgcgctgg aaaactttgc
     6121 cggatgcgtc gtcgtcatca gccacgaccg ctggtttctg gacagaatct gcacgcacat
     6181 tctggcgttt gaaggcgaca gcaaggtgct gttcttcgac ggcaactatt ccgaatacga
     6241 ggaagattac aggaagcgat acggcaatgc cgccagtcag ccccatcgta tcaagtaccg
     6301 ccagcttacc agaaggtaag cgggaaggac cattcgattt gcatcaatcc gttgcagatg
     6361 ccccgatggt tatcctgtag caatcttccg gcttccggtt atttcttctg aggctttgat
     6421 tttattacaa gtttcacctg acttcacaga cggaaaaacc cgatttacag atcttgcggc
     6481 acttaacttg aaatatctga atcttattaa attcgcgttg cagccgcctg cagtcaataa
     6541 tacaataccc aatcatttca agtacatgga aattatttct tgacataatc gtcataataa
     6601 gtcaatagca caaacaattg ttcaaaggtc aggagtgaac ttattgaata aaaagcgttt
     6661 agttttactg gttattctct ttattacgat cgggttattt tctttgcagc agccttcgga
     6721 gaattctttt cggggggatg ccgttgtatc ggaaccaacc gctccggaaa agggaaccag
     6781 gccgttaaaa tccataagcg acaaattcga aaagggagaa acactttcct cgatttttaa
     6841 aaaatacggg atgcacaccg atgatctttt cgaactgaaa agtgcggtgg ccagcattca
     6901 gtcgctgcgg gatattaagc ccggcaagcc ttacagtttt acgctcaata aagataatta
     6961 catcagctct tttacgtgcg gcatcgatga cgacaccatt ttaaaaattg aacgggtgga
     7021 cggatgtttc caggcgcaaa aatataacat tccttatgag acccgtattc tgaccatcgg
     7081 cggcacgatt caaaacacgc tggtttccgc gttcggcaac gagcgggaag attacctgct
     7141 ggccctgcag gcggcggaca ttctggcctg ggatatcgat ttcaacaccg atgtgcgcaa
     7201 aggagataca ttcagaatca ttgttgaagg cctgtattcg gaagggaaat tcaaaaaata
     7261 cggcagaatt ctgtccatcg aatttgtcaa cggcagccag acctacaacg cgtatctttt
     7321 cgaacaggac ggcaagacgg attattacaa tgccgacggg caatcgctgc gcaaggcctt
     7381 tttaaaggcg ccgctgagtt tccggagaat cagttctcat ttcagcagaa gtcgcttgca
     7441 tcccatcctg aaggtgcgcc gtccccatca cggcattgac tatgttgccg ccacgggaac
     7501 gccggtcagc acggtggcgg acggacggat cacgtttgcg ggttataaat ccggttacgg
     7561 taaaatcgtt atcgttaacc acgccaacgg ctatcagacg tattacggtc atttgtcccg
     7621 catcaaaagc aatattcgcc cagggaaaaa agttcagcag ggtgacctga tcggaaatgt
     7681 gggagccacc ggtttggcca cgggaccgca cctgcatttt gaaatgagac agggaggcag
     7741 ggcgattaat ccgctggcgg ttaaaaaagt tgccggcagt cctgtcccag cggcacaaat
     7801 ggccgaattc aaaaacacaa ctcaaaccat gaataatatt tataccgtcg ccctcttctt
     7861 cgatacgaat aacacgcaac ggaaaaatca cagcctccag ccggcgctct tgtatgcttc
     7921 cctggcgtcc tcgtctccgc agggcaagtt tcgtaaaaac tgacgcggga tcccttcaga
     7981 aaccttccgc ctgctttatg aaatacctgc catttagtgt tttgttttga tggtgcgaaa
     8041 cgtcaataat gccgccacca gagcaggaat aatcagaatc gccatgccga tattgacaaa
     8101 gttctgtgcg ggaacaacat cagaaaaata gagaatcagg atcaacagcg cgcaaaccat
     8161 attgccgatg ataaatacct ttccgaaata agcgttggct tcataccaga caaaatcatt
     8221 ttccagcgtg gctttaatgc gaaaaccgta aatcacattt ttcggcactt tgcgtagcgc
     8281 cagaggaacg gcaatcatca cgaaaataaa attgcagatc aggacggtta tcagtgtttt
     8341 aaacggcatg tcatatctcc ttcatctgag aatgtcgttg ctcatccgct ttgaataatt
     8401 ctttattttt taaaacacat cggctagata atcaagcgac gaaatacatg aggtctgttt
     8461 ttgaataata caaaaattcc cacggtcaag ccgtttgttc atcatctgcg tccgcgcggt
     8521 ttatccaggc ggattcccct gatcgaaaca ttgcgaaact acaggcgtga atggctgatg
     8581 cacgacatcc ttgccggtct ggtgcttacg gccatcctgg tcccggtcgg catggcttac
     8641 gcatcggtct cgggattgcc ggcgatatac ggattgtatg ccacgatcat tccgctgatt
     8701 gcttacgcga ttttcggacc cagccggatt ctgattctgg gacccgactc ggcgctggcc
     8761 ggcctcatcg cggcggccat cctgccgctg gccgccggca ataccgataa ggccatcgcg
     8821 cttgccggcc tcttggcaat cctcgcggga gctttttgca ttctggccgg tcttgtgaag
     8881 ttcggtttca ttactgatct gctttccaaa ccggtccgca tcggatacct caacggaatc
     8941 gcgcttacgg ttctgatcgg ccagttgccg aaagttttgg gttttcccgt cggcggcaat
     9001 aatattttgc aggaagttaa cagcctgata cagggcatcg ccggcggtca gatcaactgg
     9061 atgtcgtgcc tcatcggatg ttcctgcctg atgattattc tgggttgccg gcaatggttc
     9121 tcccgcattc cgggggtttt catcgccgtg gccggcgcaa cgatcatctc ttcctggttt
     9181 catttatccg caagttacgg cattgccgtc gtcgggccgc tgccgcaggg actgccgcaa
     9241 ttccagattc cctcggtgag ctttgctgaa tttaaagcgt tatttgctgc cgcgttcgcc
     9301 atcgcgctgg tttcctttgc cgatatgagc acactctcgc gaacctttgc actgcgtgcc
     9361 ggtcaggaag tggacagcaa tcaggaaatc atcgcgctgg gcgcagccaa tatggccgcc
     9421 ggattatttc agggatttcc cgtgaccagc agcgcctcac gcacaccggt ggccgaatcg
     9481 gcgggagcca aaactcaagt gaccggagta gtcggcgcgg tatgcattgc cctgcttctg
     9541 attttcgcgc cgaatctgct caagaatctg ccccaggcgg ttctgggcgc cgtcgtcatc
     9601 tgcgcttgca tcagcatcgt tgaagttcgc gccctggcgc gtttgtataa attgcggcgg
     9661 gaagaattcg ttttctctat cgcgtgtttt ctgggcgtgg tttccctggg gatcatcaag
     9721 ggtattttta tcgccatcgg cctggcgctg ttttccttca tctggcgggc ctggcaccct
     9781 tacgatgccg tattgggaca tgtcgaaggt ttaaaaagtt atcacgacat atcccgtcat
     9841 cccgatgcca gattgattcc cggcctggtc ctttttcgct gggacgcgcc gctgtttttc
     9901 gccaatgccg aagccttccg cgagaatgtg cttcatgcca ttgccgaagc gccgacaccc
     9961 accaagtggg ttgttgtaac ggccgaaccg attaccgatg tggacaccac tgccgcagac
    10021 atgctggccg aactggataa tgttctgcac gaatcgggca tggcattatt ttttgccgaa
    10081 atgaaagacc cggtcaagga catgctcaag cgctacggac tttttacacg attcggcgag
    10141 gagaatttct ttccgaccat tgaacaagct gtggaacgct atctgacact gcacaaaatc
    10201 gactggccgg aatcgcataa acgatagcga aaacattata ttgattgctt acttcaaaca
    10261 ggaaatattg accggcatat gaatctttgc cgctgtgttt tatcctcagt aaatctgaca
    10321 tgagcaaggt aaaggattgc cccttttctt tgtcattccg tcgtaggccg gaatccagtt
    10381 tttttctgga tgccgttttg caacggcatg atagcatctg tcttttcgta tccccgaatg
    10441 aacttgtcgg gctatggtga cctctgggta gatccagctt tatttccaag ttaaaccgag
    10501 tcttttatcg cgggatgttg tcccaatcct tttttcttga caatgtttaa tgctcccggc
    10561 tcattggaaa taatccagtt ttccagatca aatagaattt tttggacgcg gggcagtttc
    10621 atgagttccc ggatcatgcc ttcgataacg ggaaggctga actcgccctg ggactcccgg
    10681 acgagattgc cggacatata gaccgttcta tggacaaagg aatagctgat tttcgtcatg
    10741 tcggcattgt ggctgacgag aacctgcctg atttttcggt tgatctcata tcgaagcaat
    10801 tcagacgaca tgggcacttt cagcgatgtt gtgttttgta ttgctaagac agcttaattt
    10861 ttcttctttc cggattcggg ttttgtcgat agaaaatggc aatgttgccg atcaggccga
    10921 caagttcgct tttggtttta ccggcgattt cctgcgagat ttctttttta gcttccttga
    10981 attcgccgaa ttttatcttg atgagttcgt ggtcgtttag agcagcgtta accgagctga
    11041 tcagttgcgt tgtcgagcct ttggcgccga tcatgacgac cggctttaag tgatgcgcct
    11101 gtgcgcgcag atattttttt tgcgatcctt tgagtttctc catgattttt cctttcagga
    11161 tggaacatca ttaccacaaa taacccttta ttgtaagact caaatttcaa aaacgcagtg
    11221 tattgaaatt tgtaagtcga acaatcccgc tataagcgga gggtatcata ccccatacct
    11281 tggggcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ttcatacccg
    11401 tgattaaaag actttacaaa attcctgtat cttgattaaa gtacagacac tttattcagg
    11461 gggtgggacg tgagcaaagc aaatttaccg caatacgatt gcgatacgat aattatcggc
    11521 ggcgggccgg gcggttatac cgcgggcatt tatgccgcac gcagcggtct gaaatcgctg
    11581 ctggtggaag gtgcttcaac ggtcagtcag ataaccatta ccgatctgat tgaaaactat
    11641 cctggcattc ctgatggcat caacggtttc gacctgatgc agctattcaa gaaacaggcg
    11701 cagaatttcg gcctggaaat catcgccaat gatgttcact ccatcaaaaa agccggcgac
    11761 aacccggcga tctgggaaat tgccgtgggc gataaatcct acaggacgtt aagcgtgatt
    11821 gccgccaccg gcgcgcagtg gaataaattg ggcgttcccg gcgaagaaga atttgccggc
    11881 aagggcgtat cctattgtgc cacctgtgac gggccttttt accgcaacag ggatgtcgtg
    11941 gtcgtgggcg gcggggatac cgccattcag gaagccctgt tcctgactca ctttgccaaa
    12001 aaagtttccg tcattcaccg ccgcgaccgc cttcgcgccg cggccatcct gcaaaaaaga
    12061 gcttttgcgg aaaagaaaat cgaatttgtc tggaaggcaa aacttgccga gatcgccggt
    12121 caggagtttg tcaccggtgt cagggtggcc gatgtggaat caggcaaaat taatcatatc
    12181 gaagcggaag gtgtttttat ttttgtcgga ctgactccca acaccgctct cttccgcgac
    12241 gttttgaatc tggataaagg cggttacatt atcacggatg acaacatgcg cacatccgca
    12301 cccggcatat ttgccgcggg cgactgccgt gccaaacagt tccgtcaggt ggtcacagcc
    12361 gcgggcgacg gtgccaatgc gatttacagc gccgaactat atgttgatga actcaagggc
    12421 cagacatatt aagctgtcac catatcgaac gcagagtgta gtccggcttt gccgggaaga
    12481 tatctaaaac acaaggcttc tcctcggtat gcgccgggtc gaagtgacaa aatctatgaa
    12541 ttaggcagac agaaacacaa catggaagct ttcatcaata cctggcagca attaccctcg
    12601 catattaatc ccgctctttt caccatcggt tcttttcaat tgcgctacta cagcctgatg
    12661 tatctggttg gattcgccat cgtttatctg ctgaccactt atcgcataag caatgaacat
    12721 tacgaataca agaaggagat gcttcaggac tatctcatct ggggtatgtt cgcgatgatc
    12781 gtcggcgcgc ggctgggcga agtgttaatt tacaattttt cctatttttc tcagcatccg
    12841 ctggagattt ttctgccttt cagcttttcc aacggaattc atttcaccgg tatcagcggc
    12901 atgtcttttc acggcggcgt ggtcggcgta atgatatatt cgatctattt tctgcgcaaa
    12961 cgacagatca acttctggcg gttcgccgat ttgattgttc cttcgattcc gctgggctac
    13021 acgttcgggc gtctcggcaa ttttcttaac ggcgaattgt ggggacgcgt aacaacgatg
    13081 ccctggggaa tgtattttcc ttttgatccg acgcagcatc tgcgccatcc gtcccagctc
    13141 tatgaagcct tctttgaagg aatttttctg ttcgtcattc tgtggctgtt aagaaagaaa
    13201 aagtttgccg acggttttct tctggggctg tacattttcg gctacggttt tgtccgtttc
    13261 atgattgaat ttttccgcga acccgacttt atggtcggtt cggtcagtat cgggcaggtt
    13321 ctttgtgtgt tgatgatggc ggccggactc attattgttt tatggcgcag acaaattgcg
    13381 tcaccagcca ggtaacattg gaggtcgtat gggaaaacat ttaattattg tcggcggagg
    13441 agccggcgga gcgtccgcgg cggctgaagc caaacgaaat gatccagaac tgaatgttgc
    13501 gattctgcaa tcgggaaaat tcattgccta tgcgtcctgc cccacacctt actatatcgg
    13561 cgacattatc aaagatgaaa atagactagt ggcgcgcacc ccggaagaat tccgtaaacg
    13621 gggcgtggag gtgcagcttg acacggaagt cgtcaatatt aattgtaatg caaatgttgt
    13681 cgaaacaagg gacaacctta tgctgccgta tgattatctg gtcatcggta ccggcacgaa
    13741 cccctttgtt ccggacattc ccggccggga actgccaggt gtttatgtac tgaacaatct
    13801 ggaaaatgcc attcacatta aagcctggct gcgtgaaaga aaagccaagc gcgtggttat
    13861 tatcggcggc ggtttcattg gtctggaaat gagcgaagcg ctgcacgaag tgggaatttc
    13921 cacaaccatc attcacaaac attctttacc tgcaaatcgc tggagtaaag aactatcgtc
    13981 ggtgatgctc gaagaacttc aatcgcacgg cgtggaattc atcggcaacg cccaggcaaa
    14041 agccattgaa aaatcatccg gcgattcttt gaaagtgatt accgacgcgg gcgagtggca
    14101 gggcgatatg gtgctgctgg ccgttggcgt ccggcccaat gccgcgctgg ccaaatctgc
    14161 cggtcttacc atcggcaaaa ccggcgcgat tgccgttaat ttcgcccagc agacatccgt
    14221 tgaaaacatt tacgccgtcg gcgactgctg cgaatcttgg aaccgcgtca gccgccgctg
    14281 ggtcaatatt cctctgggcg acatcgccaa taaacagggg cgtgttgccg gacgcaacat
    14341 cggcgcgaaa ccgttgacgt tcgacggcat tgtcggcgca caatctttcc ggctgtttga
    14401 tctggaatgc gcggccacgg gtctctcgga aaaagaagcg cttgccgcag gctttgtgcc
    14461 catcagcaac atcacatggg gcagcgccat ggcaccggca atgggcatga aaaaaatagg
    14521 gctgaagctg actgccgata aatcctcggg aaaactgctg ggcgcgcagg cggtgggtgt
    14581 tgccggcgcg gtgggacgga tcaatgcgct gtccgtcgct ctctggaccg gattaaatat
    14641 tgacgaagtc ggttatctgg atctggctta cgcgccgcct ttcagcgcgg cgtgggatat
    14701 tattcacaac gctgctcagg ctttgcgccg gatgatctga aaggaaaagg acttatcaat
    14761 gaccctctgg tggacgatct tcggtgtctt tatccttgcg atgctggcgt tggatctggg
    14821 cgtttttaac cgcaaggctc acgtcatcaa aatgaaggaa gcgctcctat gggtttcctt
    14881 ctgggtatcc cttgctgttg cgttcggtgt gggtgtttat tatttttacg gccactccaa
    14941 ggcgctggaa ttttttaccg cttatatcat tgaatactct ctgagcatcg ataacctttt
    15001 tgtgttcctg ctgatcttcc ggtttttcaa cgtgccgcaa gcctatgaac acaaggccct
    15061 tttctggggt atcctgctgg cgctgattac ccgcgccgcg ttcatttttg caggcgtcgc
    15121 gctcattaac aaattccact ggatcatgta tcttttcggc gctttcctga tttataccgg
    15181 catcaagctg gcgatgaaca aacaaacgga agtccatcct gacaaaaaca tcgcgataaa
    15241 aatgctgcgt tatgttgttc cggtgaccaa agaatttcat gaagccaggt tctttatcat
    15301 taaaaacgga aagcgtttcg ccacgcccat gctggccgtt ttgctggcgc tggaaaccac
    15361 cgatattttg ttcgcggttg attccattcc ggcggtactg gcgatttcca gagatccctt
    15421 tatcgtttac acctccaatg tctttgccat cctgggactg cgttcgcttt tcttcgccat
    15481 ttccgggctg atgaaactct ttcatctcct gcactatggc ctttcagcca ttttgagttt
    15541 tgtcggcgta aagatgctca ttgcggattt ttaccatata ccggtgggaa tctctctgac
    15601 ggtcatatcg tccattctcg tcatatccat tgtcgcgtcc atcatctggc cggatacaaa
    15661 atatgaagac attccgaaca tcaccatgaa ggattaacgc tatgcaaaac ggatttttga
    15721 cgccttcaca gattcagttg aaaaaatggg ccagactgga cgacgcgaag gcgcgccatg
    15781 aagaaggggt gtttctggcc gaaggcgtca aggtcgtcga agaactgctg aaaagcgact
    15841 ggcaaattaa agcattgctg gtcatgccgg aaaaaataaa attctggaag aagcttgatc
    15901 tttcccggga ggaagaaatt cccgtctatc agttgaagcg aacacagtgg gaaaaaatcg
    15961 gacaggaccg ggaaccggaa ggcatcatgg cgctggtaga aatgaaacag gcgccggatt
    16021 tttcctcgtg gcttgcacaa acttccggca atgttttgat cctgcatgaa atcaacaatc
    16081 cgctcaatct cggggcgctg gcccgcagcg cgcagtggtt tggatttaac agtatcatac
    16141 tgagcgccaa ctccgtggac ttcaccaatc cgaaagcnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnccg tccatcttga cctggcgctt gtatttgctc aatttcacac
    16321 atctgtctat atcaatgaag cggggcagaa gctctatatg acttttatga agataatagc
    16381 ggttaactcc atcggacatc gcaaacacat aattattgtt cagaacgatc tgttcccaaa
    16441 gcatatagga aagatctccc gtgcccggac gcgacgcttc aatacacaac accacgggct
    16501 tatatcgcgc aagatcaata ctctcgagaa cctgtttctc aaagccttca acatcaatgg
    16561 tgatgaatgt aatttcacga acggatatct ttccaagcaa attattaagt gtcgtgacag
    16621 gaacaacaat ttccttgaaa agaattccgt ttttcttttg aatatccacg atttccttat
    16681 cgaaggttga aagcggcatc aatgcatccg attggtatgc ctgctgataa aaaatcatac
    16741 gtccttcagt tgtggcaata ccgacattat aattgatatc gcgctgccga aaagagcgaa
    16801 gttgtaaata caactcggca ttgggctcaa tattgatgcc atgccagccc ctttcataga
    16861 agaatcgagt tacattaaag atattgggat cgttagctcc gacatcgata tagaaaccat
    16921 cggcgacatc ctgaaaaaca tgggcaagta tataatcctc ataagtctgg ctgtaataaa
    16981 tcccgttttc aaatttagaa ggaggacaat cgcacacccc gttttgttgc cggcaattga
    17041 ccagagaaaa cataacggaa atcgcaaggc acaaaaaaac aaggatttta atttttgatt
    17101 tcattgtttt ccgcctcgcg taatttgttt tttatgattt cggatggaaa tatcttgcga
    17161 caaatcatca aaaaatacca tcttttattt ttctgaaatt catgcctttt cacgattcca
    17221 tgaataatat gaaaccggat cggtgtaatt gctaaaacaa tttctttgtt gtttgttttt
    17281 tcttgatttc gtaaggtcaa acaaataata tgctatccaa tgcaaataat gacagatttg
    17341 ttcatcgtcc aaagccgatt cgctgagttt gccgcaggct gcggtgatca tgatgtgtga
    17401 atgtgttaaa cggtaattaa aacacgtcac accggcgaag gccggtgtcc agtaggatca
    17461 tcgtgaatgg ataaaagggc aacaccactg tcattcccgc atgggcctag cgggaatcta
    17521 atgacactcg ctaagaacga gctacgcgat gttcgaagcg taagtggaat tatcccagga
    17581 gcgtagcgac gcgggatgtt ttgcttcact agttttgcaa tgataataag gaattgaaac
    17641 aatgaaatcc tacattcaag tatccac
//
LOCUS       DAONQZ010000047        17388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3681_length_17388_cov_15.972192, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000047 DAONQZ010000000
VERSION     DAONQZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_17388_cov_15.972192"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1730)
                     /locus_tag="PLR13_08195"
     CDS             complement(153..1730)
                     /locus_tag="PLR13_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC19356.1"
                     /translation="MNKTAKLIEIDLNPEFRRALELMEETDKNIFITGRAGTGKSTLL
                     NYFYHYTNKNVVLLAPTGVAAVNIGGQTIHSFFQFKPNVTLAAIKKKKGADKDKKSLY
                     KKLTTIIIDEVSMMRADLLDCVDKFLRLNGPEFKRPFGGVQMIFIGDLYQLPPVVTAA
                     EREIFRSHYRTPYFFSAKVMDELDMEFVELEKVYRQKDDEFIRLLNAIRNKSITDGDI
                     ALFNQRCDPSFEMSADDFYISLTSTNDLADSINEQRLAQLSGKVWKAQGFVEGEFGAE
                     SMPTAVELKLKKGAQIMLLNNDAYGQWVNGTIGKVKKFAKNEDGDDVIVARLDNGETV
                     EISPYTWKIYKYFLKDEELHSEGVGSFRQYPVRLAFAVTIHKSQGKTFERVVVDVGRG
                     TFAHGQMYVALSRCTTLDGLVLKNPLKKSHVLMDWQVMKFLTGLQYAKAELNCTREQK
                     IAMLEEAFHEKQVVEIVYLKAKDEKSTRKIRPLRVEEMEYNGHPYLGLYAYCLMRRQN
                     RVFNVDRILKIRLPSND"
     gene            1969..2715
                     /locus_tag="PLR13_08200"
     CDS             1969..2715
                     /locus_tag="PLR13_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQC19357.1"
                     /translation="MPIHMDTRNEVAILAMDNSPENAFTSDMIQQFNATLDRIEADDK
                     IRGVVVTGADGQFFSSGMDLQYVMKLEVKAIKIFFKTLFTFFHRTFVFPKPMVGAING
                     HVVASALAFSMCMDYRVMQKEKAVCTFPEIDVSITPPSGCLAMVKYVIGERMTDLAFL
                     SGRKFDGAAALAAGMVDELAESRSVIDRAVEVATELGAKKPSLFAEYKKRFRGETADR
                     MLAEDMRYIDKEMDVTMFQNCDLSCLRSLP"
     gene            2742..3476
                     /locus_tag="PLR13_08205"
     CDS             2742..3476
                     /locus_tag="PLR13_08205"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19358.1"
                     /translation="MSAADYGKFLNRPVPELPENILNKLKNNPNISKIAFADANQIIH
                     DENLQNEIGFAELNNKEYLVAMSTELPNTDSKMIDWWFWWQAQESERYQLWFPGEHKS
                     ISYKKSQRAYFSNKYQAFLENTQYPVERVGKITVELSIRFVDPVRFGIKDKEKVLKHN
                     GTIICGYVGILKGIIQHTKMLHYFKPNKKGLILISRFWLGNGLPRVIRGIAANKDQAF
                     AMAKHCWIEYTRLGQILPEIYHQAKT"
     gene            4072..4845
                     /locus_tag="PLR13_08210"
     CDS             4072..4845
                     /locus_tag="PLR13_08210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19359.1"
                     /translation="MSGDNKSIHEFDFKLICEYFSSRERQGPGSPEVTVKALSFIDNL
                     THDSKIADIGCGTGGQTMVLARHTPGHITGMDLFADFIDIFNANSRKLNLQDRVQGIV
                     GSMEHLPFHNEELNLIWSEGAIYNIGFERGINEWRSFLKQGGFLAVSEASWFTEKRPA
                     EIDRYWKDAYPEIDVISHKVGQMQKAGYIPVATFVLPENCWTEHFYAPQVLAQEKFLK
                     KYADNKTAEDLVANERHEAMLYNKYKAFYGYVFYIGKKI"
     gene            4951..5466
                     /locus_tag="PLR13_08215"
     CDS             4951..5466
                     /locus_tag="PLR13_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19360.1"
                     /translation="MMDKFKIRICHHEDIGILTETIRKSFRDVAQRFGLTRRNAPSHP
                     SNCMLNWIRYDMERGVTYFIAENESRTAGCVACEQASAKICYLERLAVLPEERNQGLG
                     KALVERVLSNARELGAGTVSIGIIADHAELKNWYRKIGFVEGETRTFINLPFLVTFMS
                     YNLNENSLREK"
     gene            complement(5521..6141)
                     /locus_tag="PLR13_08220"
     CDS             complement(5521..6141)
                     /locus_tag="PLR13_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQC19361.1"
                     /translation="MKKIVQYAAMITLAVLFAVYPFQPASAQVGLYLGGWGGYTIHPN
                     ATEDDWDYYDDVDLDFDETSAWGAKIGYTNPFFKYLALEAEYLYLNPEVEDDYVLDGD
                     VKMSNLMFNIIGKYPLGIIQPYFGLGLGFSYADTSWDGIRGIDDGDYTSYAWQIMTGL
                     EIFLTSEIAVDVGYRYFATEMEFDHEDSDDVTFSTGMVTLGLKVFF"
     gene            complement(6229..6798)
                     /locus_tag="PLR13_08225"
     CDS             complement(6229..6798)
                     /locus_tag="PLR13_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQC19362.1"
                     /translation="MKVLGIMGSPRIGGNSDILLDDALAGAEEAGARVEKIVLVKKKI
                     SGCLDCKKCNDTGLCVVKDDMPAIHKKILEADAIIHSVPVYFWSMTAQMKAYLDRWCA
                     LFDATWSWQKSYYPKMKGKRIGLITVCGDPNVHTADPIVHSFKSTSDMTKMRWMGAVM
                     ASANDKGEIVKDEKARKKAFKLGKKAATP"
     gene            6932..7747
                     /locus_tag="PLR13_08230"
     CDS             6932..7747
                     /locus_tag="PLR13_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQC19363.1"
                     /translation="MPESFHFFSYHRREQDDFTVIAHRGASAYYPENTMPSFEGAIAM
                     GADMAELDVQLTADQEVVVFHDEKISRCTNGRGRIADYDLAYLKQLDAGKWFDKKFKN
                     TRIPELADVLALCKNRIAVNIEIKTEAVDDIIAGGIEEKCLQLVEQYGMKKHVVFSSF
                     DPRAILHLKQIDKTAPVAVLFEKKLYGSRLPSDIVKSLHADAFNCSASELNKKWLADL
                     HSNNIPVNIYTVNNEKKMRQYLKLGVSGIFTNKPDVLKNVLADGDQKSKKTNL"
     gene            7744..>9284
                     /locus_tag="PLR13_08235"
     CDS             7744..>9284
                     /locus_tag="PLR13_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQC19364.1"
                     /translation="MNISKDLVNFIMFSLDTLIQTAGIDIRLHGLEKVPDQPVVYVIN
                     HFTRLETVLMPYLIKKHLHKEAVSLAHESFFSGKMKDFMDNVGAVSTADPHKDKIFIH
                     ALLSDRHPVIIFPEGQIIKDKKIVEKGKYIVYNTGIRRPPHSGAARIALLTEFIREKL
                     RTLKARNDMASIARIASRFDFSGNDLEKIIRKETYIVPVNITYYPVRARENALSRLAG
                     KFFKNMSARLQEELEVEGPIVLGKVDIDINFGRPIAIKRYIHSKWKMKRMMADGNVYL
                     NTGDFKRISAFRQVCVRLMYDYMNAIYGMTTVNHDHIMSYILTSYPQGSFSENDFKKR
                     IFLAIEHLRKIALPNCHTSLYKKQFYLLTDDQHEKYESFMNEAVTNNYLRRENGVITK
                     NKERFSVPSDFHTIRRDNIIEVLRNEIKPLRHFTRIMEWQKFIPAFFIKWNVRNTFLK
                     LEKELFHQDYEQFYLPGESKPKNIGEPFFLKRFFSRKGIILVHGYMAAPEEIRPLADY
                     LHQNG"
     assembly_gap    9285..9384
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9385..10079
                     /locus_tag="PLR13_08240"
     CDS             <9385..10079
                     /locus_tag="PLR13_08240"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC19365.1"
                     /translation="AAPEEIRPLADYLHQNGYNVYGVRLRGHGTAPEDLAIRNWEKWY
                     DSASRAYIIMKNSVKTFSIAGFSMGGGIALLQAANKPGRFASVISINAPMVLRGMASK
                     FAPAVVAWNTLLKKINVDKWKMEFIKNDPENPDINYLRNPVSGSYELEKLMKRVDHHL
                     QNIADPALIIQASDDPVVDPCSGREIFNKIDSGNKKFFMVNANHHGILRGKEATEVNA
                     KVLAFLSESASK"
     gene            10170..10517
                     /locus_tag="PLR13_08245"
     CDS             10170..10517
                     /locus_tag="PLR13_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQC19366.1"
                     /translation="MIEQWMKEIKRTAPANRLGMILSHNGIVRATSKTGESVQAMHLS
                     YDKTKLEKAVREFKGKEGIVDIRVWINEGHLKIGDDIMNVCVAGRFRKDVLPVFQELI
                     SMIKTEIVKEEEI"
     gene            complement(10615..11226)
                     /locus_tag="PLR13_08250"
     CDS             complement(10615..11226)
                     /locus_tag="PLR13_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19367.1"
                     /translation="MIRHAVVMVSAICFWGAAFLTVPAYADYRIILKNGETISVSNYE
                     MDGDNIVFNKYGGLVSFSRSGIAKIEEDNIEEDIPEENMAEDDANEADDSEQPAKNKV
                     SKKSGQKSEDPKNDREKKIAECQRILKMKKDSMDIYCWQAASAKVSAAPNLPQTNQAR
                     VSEKTGKQFQDHVQSNVDAFKANSTCDYYKRKVAEQEKLCNGL"
     gene            complement(11330..12523)
                     /locus_tag="PLR13_08255"
     CDS             complement(11330..12523)
                     /locus_tag="PLR13_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19368.1"
                     /translation="MLTSDLDTTRLAIIGPGRLGTAFAYKFGRDNKRVAIYYHDAEVC
                     KAINRDHLNPLHLTEDLAGRLGGMEKVPRLADKVYATNDLEHIVENNDFIILTITMNR
                     LPEFLNYLKPMIDKKAGDTCLISPIKGLISDEISKELITPSQLINNSLYNLKHKYQIG
                     CIGGPFFDMDIALGNPVCLTVAGKKRIANIIREELLKFNRKEINSYYNFDIVGVEACG
                     ALKNVVANIKGLADSLQLGDSIPGTIFARSGVEIRSLSRLMGGSFQAFHSQAGVGDMY
                     VTVSSLASKNYRYGKYFYELYTGNPIETNLRVLEKIDGTPEGPNTVRNVHKYLEKKNM
                     YSPLFSCAYHIFNEAGEKGAIRDLIIQACQYDKRNKEYIDAFSRLMYRILPDFWYRRD
                     KSWFD"
     gene            13035..13700
                     /locus_tag="PLR13_08260"
     CDS             13035..13700
                     /locus_tag="PLR13_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HQC19369.1"
                     /translation="MEKQTLGKEITQEFHDGKIKEKKYVRQGKTEGEYFSFYHSGQIL
                     AHVNFIRGKKNGPAVIYYHDGHIREKATFVNDRMEGEYVSYFENGHIREHEFYRNGKL
                     DGKYEIYYKTGQLKLVETFRNGKFEGEYIAYYKNGQIKEKGFFKNDKREGEYIAYYKS
                     GQIREKATYKNGNCVGKFLAFEEEISGSGETNGSESDTEIWDEKDVANLLEELKHFDK
                     KQK"
     gene            13727..14578
                     /locus_tag="PLR13_08265"
     CDS             13727..14578
                     /locus_tag="PLR13_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQC19370.1"
                     /translation="MISNILIPTDGSANSQTALDYGIYIARKLAASLTGLYVIDVNLI
                     QGPMVTDISGSIGMPPYEGFFESIEKSLNEKADFILKEFEERCRKSGVKAATDRKTGK
                     ISPIIIEEAQKADLILMAKKGEHFHLKEGGLLGSVAESVTRNSGKPVLVTPEKFVEIE
                     SMALAFDGSESATKALGLSLNLSHKTSWPLTVVIVSPDEKKAEALSGKVDKMIQQNPD
                     EASIDCETIILSGKEQDEIVKFIREGSVELMVMGAYGHNRLRELLLGSTTSHVIHKSP
                     IPVLLIR"
     gene            14633..15832
                     /locus_tag="PLR13_08270"
     CDS             14633..15832
                     /locus_tag="PLR13_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19371.1"
                     /translation="MEHRYGKNILPLSTFDNENFIGSPPLEILFFYKKDLPILKLKES
                     LLKTIHYYNLFSSRLIMIGDHKFALLYCTDGAVANDLRPVDACFDDSRIAEIRKEMVH
                     VQTLPGEPLFAVTGIPFKNGILAGISCSHAVADGISLLLFLYAWMCITDGKNFLPPSP
                     QRLFQGPLAHFDPTDRALVPPLSALNDQIREKVDIHNVAEIFTATEYFTDDFLNAIKA
                     EAEKENYDISNHQIMTAFLLKKYHCKILPDTDRIVLKNPINLRDIHPDIDPMYIGSSN
                     FNNFIQFTKEEIDRLSISQIAYRIKKSIAAVRNESYAQEIAHLSPYGIEINMDVFRRN
                     RPPYSVETNVVSSNLTHLNDLESMFVGPETGSLLHIGLAVQTGFTMLKEKSGRIFAQI
                     TGRHPLT"
     gene            complement(15899..16666)
                     /gene="surE"
                     /locus_tag="PLR13_08275"
     CDS             complement(15899..16666)
                     /gene="surE"
                     /locus_tag="PLR13_08275"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQC19372.1"
                     /translation="MRFLLTNDDGIYAKGLSALYKELSKEADCLIVAPETEQSAVGHA
                     ITISRPLMVRRATKNNQFLGYAVCGTPADCVKLGIKELSDQPVDLVVSGINRGGNAGI
                     NVLYSGTVSAATEAAIMGFPSMAVSLDTHKEADFTFAAKFARKMTRMILNHREQLLGS
                     AINVNVPCLPQGKIKGVVVVRQAQTNIVEYFEKRTDPRENTYYWFAGESSSATRQKDT
                     DVGALTSGFITVTPIHFDLTRHDLLDQLKDALEKNRF"
     gene            complement(16730..17314)
                     /locus_tag="PLR13_08280"
     CDS             complement(16730..17314)
                     /locus_tag="PLR13_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQC19373.1"
                     /translation="MNMLPFRKKIFYLPVLVFLILGFATGVFAAGKERQANQCKTVLL
                     TNEEFLPALLNAIDGAQDEIIMSIFSFKAGVHKNSYPDRIAGHLASAAKRGVKVFVVL
                     ENTGDPSDALNIQNRKAGRFLEGKGIRVYFDSPRKTTHTKLTVIDQRLIFLGSHNFTQ
                     SALKYNNEISVLLEGRDLALNARNYILGIMKEGT"
BASE COUNT         4880 a         4079 c         3874 g         4455 t
ORIGIN      
        1 tgacacccgc tgatagttat atcctttgag agcgcatgaa gagatgctcg aaaagtaagt
       61 tgaactatac cagccgcgta acaccatggt aaaacaagcc ccgattgatc gggatgaatt
      121 tcgtccctgc cgcctgatgg cggcggcatt attcaatcat ttgacggcaa acgaattttc
      181 agaatgcggt ccacgttaaa aacccggttt tgcctccgca taaggcaata agcatacaac
      241 cccagatatg gatgaccgtt atattccatt tcctccacac gaagcgggcg aattttacgc
      301 gtcgattttt catctttcgc ttttaaataa acgatttcga caacctgctt ttcgtgaaac
      361 gcctcctcca gcatcgcaat cttttgctcg cgggtacagt tcaactccgc tttggcgtac
      421 tgcaaacccg tcagaaattt catcacctgc caatccatta aaacatgact tttctttaac
      481 ggatttttta acacgagacc gtccagcgtt gtgcagcggg atagcgccac atacatctgc
      541 ccgtgggcaa acgtgccgcg acccacgtca acaaccactc tttcaaatgt tttcccctgg
      601 ctcttgtgaa tcgtcactgc aaaagccagg cgcaccggat actggcggaa agaaccgacc
      661 ccttccgaat gcagttcctc atccttgaga aaatatttat aaattttcca cgtataggga
      721 gaaatttcca ccgtttcacc gttgtccagt ctggcgacaa tgacatcgtc gccatcttca
      781 tttttggcga atttttttac tttaccgatg gttccgttaa cccactgacc gtacgcatcg
      841 ttattgagca acataatctg cgcacccttt ttcagcttca attcgacagc ggtgggcatg
      901 gattctgctc cgaactctcc ttcaacaaat ccctgcgcct tccagacttt accggacagt
      961 tgcgccagcc gctgttcatt aatgctgtcc gccagatcat tggtgctggt caatgaaata
     1021 taaaaatcat cggcggacat ttcaaaggac ggatcgcagc gctgattgaa cagcgcaata
     1081 tccccatccg taatggattt attacgaatg gcgttgagca gacggataaa ttcgtcatcc
     1141 ttctgacggt aaactttttc cagttcgaca aattccatgt ccagttcatc catcactttc
     1201 gcgctaaaaa aatacggggt tctgtaatgc gaccggaaaa tttcgcgttc cgctgctgtt
     1261 accaccggcg gcaactgata aagatcgccg atgaaaatca tctggacacc gccgaaaggc
     1321 cgtttgaact ccgggccgtt taaacgcaga aacttatcca cacaatcgag cagatcggcc
     1381 cgcatcatgg aaacttcatc gatgatgatt gtcgtcaatt ttttataaag cgattttttg
     1441 tccttgtcgg cacccttctt ttttttaatg gcagccaacg taacattcgg tttgaactgg
     1501 aaaaaggaat ggatggtttg tccgccgata ttcacggcgg caacgcccgt gggcgccagc
     1561 aaaacaacat ttttatttgt gtaatgataa aaataattga gcagtgtgga tttgccggta
     1621 ccggcgcgtc ccgtaatgaa aatattttta tccgtctctt ccatcagctc cagcgcccgc
     1681 cggaactcgg gattcaaatc tatttcgatt agctttgccg ttttattcat tatatattta
     1741 acatttttca ctgtaaaatg ttaatagtgt ttatcacaaa agattcggtg gcaaaagttc
     1801 atttctttag atctctgata acttgggata ttcttcatca agaggttttt aatcttcttg
     1861 acacataaaa tcgcgttttt tatacttcat caataataaa ggacgctcta gccaactcaa
     1921 ttaatttatt ttttaaaacg tatcgatttt tgacaaagga ggctgactat gccgattcat
     1981 atggatacca gaaacgaggt ggcgattctg gccatggaca acagcccgga aaacgccttt
     2041 accagcgaca tgattcagca gtttaacgcg acattggacc gcattgaggc cgatgacaaa
     2101 attcgcggcg tggtggtgac cggagccgac ggccaattct tttcttccgg catggacctt
     2161 caatacgtca tgaaactgga ggtaaaagcc attaagatat ttttcaagac gctttttact
     2221 tttttccaca ggacctttgt gtttcccaaa cccatggtcg gtgccatcaa cggccatgtg
     2281 gtggccagcg ccctggcttt ctctatgtgc atggattacc gggtgatgca aaaggaaaag
     2341 gcggtgtgta cctttccgga aatcgatgtt tccattactc cgccttccgg atgcctggct
     2401 atggtgaaat acgtcatcgg cgagcgcatg accgatctgg cctttttgtc cggccgcaag
     2461 ttcgatggag ctgcggctct ggccgcaggc atggtggacg aactggccga aagccgatca
     2521 gtcatcgacc gggccgtgga agtcgcgacg gaattgggcg ctaaaaaacc gagcctgttt
     2581 gccgaatata aaaaacgctt ccgcggtgaa accgcagacc gcatgctggc cgaagacatg
     2641 cgatatatcg acaaagagat ggacgtgacc atgtttcaga actgcgacct ttcctgcctg
     2701 cggtctctac cataatccgc attaactaaa tggagatttg tgtgagtgct gctgactatg
     2761 ggaaattttt gaatagacct gttcctgaat tacctgagaa tattctcaat aagctgaaga
     2821 ataatcccaa tatttcaaaa attgcattcg ctgatgccaa tcagattatt catgacgaga
     2881 atctacaaaa cgaaattggt tttgcagaac ttaacaacaa ggaatacctg gtggccatga
     2941 gcactgaatt gccaaataca gattcaaaga tgatcgattg gtggttctgg tggcaagctc
     3001 aagagagtga aagatatcaa ctgtggtttc ccggtgagca taaatcaata agctataaaa
     3061 aatcgcaacg cgcatatttc agcaataaat atcaagcgtt cttggaaaac acacaatatc
     3121 cagtcgaaag agttggaaag attactgttg agttatccat caggttcgtt gatccggtaa
     3181 gattcggaat aaaagataaa gaaaaagttt taaaacataa tggaacaatc atatgtggtt
     3241 atgtaggaat attaaaagga attatacaac atacaaaaat gcttcattat tttaaaccaa
     3301 ataaaaaagg gctgatactc attagccgat tttggctagg aaatggattg cccagggtaa
     3361 ttagaggcat tgcagccaat aaagatcaag catttgcaat ggcaaaacat tgttggattg
     3421 aatatacaag gctggggcag atacttcctg aaatatacca tcaggcgaag acataaccat
     3481 tcaatccgcc agaccgaaag cagcgcgttg gcatgcttcc gcacttgccc tgcatattgc
     3541 cagactatta cgatatgcac cacaatgctt gcggctggtg atcgccatca cttggcacga
     3601 aagaatacag gaaggatacc atgattgcat tttgcggatt ggattgttta caatgcgagg
     3661 cgtatcttgc aacgcgagaa aacagcgaca atcaacgata cgacgtggca aaaaagtggt
     3721 cagctcttta tcataaagat attaagccag gccagataaa ctgcgacggg tgcaaatcaa
     3781 acggccgctt gttttttcat tgtaaaaaca cgtgcgggat tcggaaatgc tgtaaatcaa
     3841 aaggggttga caactgcgcg gtatgcagtg actatgcgtg tgcaatcatg gcagagctca
     3901 ccacattagc tccagaggca ggtaaggccc ttgaaaaact gagatagtag taaatgcgaa
     3961 ccgggtatgg gaggcataac gggccgccgg cacaaaatgc ctcaacgtgt catttgatga
     4021 actgtcatgc ctgacggaag aggaaatgct ggaaaataaa aaggagacgc aatgagtggg
     4081 gacaataaat caattcatga gtttgatttt aaattaatct gtgaatattt ttcaagcagg
     4141 gaacgacaag ggcccgggag ccccgaagtc acggtcaaag cgttaagctt cattgataac
     4201 ctgactcacg actccaaaat tgccgacatc ggttgcggga caggcggcca gacaatggtg
     4261 ctggcccggc atacgccagg gcatattaca ggcatggatt tgtttgccga cttcatcgat
     4321 attttcaatg ccaacagccg gaagctcaat ctccaggatc gcgtgcaagg cattgtcggc
     4381 tccatggagc atcttccttt tcacaacgag gaattgaatt taatctggtc ggaaggtgcg
     4441 atctataata tcggatttga acgaggcatc aacgagtggc ggagtttcct gaagcaagga
     4501 ggctttcttg ccgtttcaga ggcttcctgg tttaccgaaa agcggcctgc cgaaatcgac
     4561 cgctattgga aagatgcata tcctgaaatc gacgtcatct cccataaagt cgggcagatg
     4621 caaaaagccg gatatattcc ggtggcaact tttgttctgc ctgaaaattg ctggacggaa
     4681 catttttacg ctccgcaggt tcttgcacag gagaaattcc tgaaaaagta tgcggacaat
     4741 aaaacggctg aagacctcgt ggcaaacgag cggcacgaag caatgctcta taataaatat
     4801 aaagcatttt atggatatgt tttctatatt ggaaaaaaaa tttaattcac aagatcaagc
     4861 atccgttgtg ttatcggata atggaccggt tgtttcaccg gatcagccga agcacggcgt
     4921 tgaatgcttc acaacaccgg caaattataa atgatggata aattcaagat tcgtatttgc
     4981 caccatgaag acatcggaat attgacggaa accatccgga aatcatttcg cgatgtagca
     5041 cagcgtttcg gcctgactcg ccggaacgct cccagtcatc cgtccaattg tatgctcaac
     5101 tggatcagat acgatatgga acgcggcgtt acgtatttca ttgcggaaaa cgaaagtaga
     5161 acggccggtt gtgtggcctg cgagcaggcc agcgcaaaaa tttgctatct tgagcgcctg
     5221 gctgtgcttc ccgaagaaag gaaccagggc ctgggaaagg ccctggttga acgtgttttg
     5281 tcaaacgccc gggaactggg cgcgggtaca gtcagcattg gcattattgc cgaccatgcc
     5341 gaattgaaaa actggtatag aaaaatcggg tttgttgaag gcgaaaccag gacgtttatc
     5401 aacctgccgt tccttgtcac ctttatgtct tataatctga acgaaaacag tctccgtgaa
     5461 aagtaaatag aaatagactt ttcagaacat cctcagacaa ttcccggaaa cgaacagatt
     5521 ttagaaaaat accttcagcc ccagcgttac cattcccgtg gaaaacgtga cgtcgtcgga
     5581 atcctcatgg tcaaattcca tctcggtggc aaaataacga taaccgacat ccacagcaat
     5641 ttcactggtt aagaatattt ccagtccggt catgatctgc caggcataag acgtgtaatc
     5701 cccgtcatca attccccgga taccgtccca ggacgtatcc gcataggaaa atcccaaccc
     5761 cagcccaaaa tagggttgaa tgattccgag gggatatttc ccgatgatgt tgaacatcag
     5821 gttgctcatc ttgacatcgc cgtcaagcac atagtcatct tctacttcag ggtttaaata
     5881 gaggtattcg gcttccaggg caagatactt aaaaaaagga ttggtataac cgatcttggc
     5941 gccccaggcg gacgtttcgt caaaatccag atcgacatca tcataataat cccagtcatc
     6001 ttctgttgca ttagggtgaa ttgtatagcc tccccatcca ccaagatata atccaacctg
     6061 agccgaagcc ggttgaaagg gatagacagc aaacaaaacg gccagggtaa tcatggcagc
     6121 atattgaaca atttttttca cagtgattct ccttttcgca aattattttg ttgtattcta
     6181 atgttttata ttccgctttt caatatgttt ttcagaataa aacaggcatc agggtgttgc
     6241 agcttttttg cccagcttaa aagctttttt tctggctttt tcatctttga cgatttcccc
     6301 tttatcgttg gcagaggcca taactgcacc catccagcgc atttttgtca tatccgaagt
     6361 tgatttaaag ctgtggacga ttggatcagc cgtgtgaaca ttcggatctc cgcagacggt
     6421 aatcaaaccg atgcgctttc ctttcatttt cggataataa gatttctgcc agctccaggt
     6481 cgcgtcaaac agcgcgcacc accggtccag atacgctttc atctgcgcgg tcatcgacca
     6541 gaaataaacc ggcacgctgt gaataatggc atccgcctcc agaatctttt tgtgaatggc
     6601 cggcatatca tccttgacga cgcagaggcc ggtgtcgttg cattttttgc aatccagaca
     6661 tccggatatt ttctttttga ccagaacaat cttttccacc cgagcgcccg cctcttcggc
     6721 gccggccagg gcgtcgtcga gcagaatatc gctgttgccg ccgatccgcg gacttcccat
     6781 aatgccgaga actttcatag agtcatcctc cttgttcata agaattctat tcaagacgcc
     6841 cgctcattta tgtatcataa atcctttcgg tatggtttgt aaacactaaa ataattgaag
     6901 aactctgctc ttaaatatgg taaaaaaaaa catgcctgaa tcatttcatt ttttcagcta
     6961 tcaccggcgc gaacaggatg attttaccgt catcgcccat cgcggagctt cggcttatta
     7021 tccggaaaac accatgccct cctttgaagg cgccatcgcc atgggcgcgg acatggcgga
     7081 actggatgtt cagctcaccg cggatcagga agtcgttgtg tttcacgacg aaaagatttc
     7141 acgctgcaca aacggccggg gaagaatcgc cgattacgat cttgcgtatt taaaacagtt
     7201 ggacgccggc aaatggtttg ataagaagtt taaaaacacg cgcatccccg aactggccga
     7261 cgtgctggca ctctgcaaaa accgcatcgc cgtaaatatt gaaataaaaa cggaagcggt
     7321 ggacgacatc atcgccggcg gcattgaaga aaaatgtctg caactggtcg agcagtacgg
     7381 catgaaaaag cacgtcgtat tttccagttt tgatccgcgc gcgatcctgc atctaaaaca
     7441 gatcgacaaa acggctccgg tggctgtttt atttgaaaag aagctttacg gttcccgcct
     7501 gccttcggat attgtcaaat cgttgcatgc cgatgcattt aactgctccg catccgaatt
     7561 gaataaaaaa tggctggcag acctccactc gaacaatatt ccggtaaata tttatacggt
     7621 caacaacgaa aaaaagatga gacaatattt aaagctgggc gtcagcggaa tttttaccaa
     7681 caaaccggat gttttaaaaa acgtcctggc ggatggagat cagaaaagca aaaaaacaaa
     7741 tctatgaaca tatcaaaaga cctggtcaat tttatcatgt tctcgctgga caccctgatt
     7801 cagacagcag ggatcgacat ccgtctgcac ggcctggaga aagtccccga tcagccggtt
     7861 gtctatgtga tcaatcattt tacgcgtctg gagacggtct tgatgccgta tctaatcaaa
     7921 aagcatctcc acaaagaagc cgtttccctg gcgcatgagt cttttttcag cggtaaaatg
     7981 aaagatttta tggataacgt gggcgctgtt tccaccgccg acccccataa agataaaata
     8041 ttcattcatg cgcttctttc cgacagacat cccgtaatca tttttccgga aggacagatc
     8101 attaaggata aaaagatcgt cgagaaagga aaatatattg tttacaacac cggcatccgc
     8161 cgtccccccc attccggtgc ggcgcgcatc gctctgctga ccgagtttat ccgcgaaaag
     8221 ttgcgaacgc tgaaagcgcg taacgatatg gcatccatcg cccgcatcgc atcgcgcttt
     8281 gatttttcag gaaatgatct ggagaaaatt attcgcaaag aaacatatat cgttccggtg
     8341 aacattacct attatcccgt ccgcgcccgg gaaaacgccc tgagccggct ggccggaaaa
     8401 tttttcaaaa acatgtcagc ccgcctgcag gaggaactgg aggtggaagg ccccatcgtg
     8461 ctgggcaagg tggatatcga catcaacttc ggcaggccca tcgcaatcaa acgttatatc
     8521 cattcaaaat ggaaaatgaa aagaatgatg gcggatggaa atgtttatct gaatacaggt
     8581 gatttcaaaa gaatatccgc attccgtcaa gtatgcgtca gattgatgta cgattacatg
     8641 aatgccatat acggcatgac caccgttaat cacgaccaca tcatgtctta tattctgacg
     8701 agctaccctc agggcagctt cagcgaaaac gactttaaaa aacgtatttt cctggccatt
     8761 gaacatttga ggaaaatcgc cctgcccaac tgccatacat cgctttacaa aaagcagttt
     8821 tacctgctga ccgacgatca gcacgaaaag tatgagagtt tcatgaacga agccgtcacc
     8881 aacaactatc tccgaagaga aaacggcgtc attacaaaaa acaaagaacg gttcagcgtg
     8941 ccatccgact ttcataccat ccgcagagac aacattatcg aggtgctccg aaatgaaatc
     9001 aaaccgctgc gtcatttcac caggatcatg gaatggcaga aattcattcc ggcatttttt
     9061 attaaatgga acgtcagaaa cacgtttctc aaactcgaga aggaactttt tcatcaggat
     9121 tatgaacagt tctatttacc gggtgaaagc aaaccgaaaa atatcggcga gccttttttc
     9181 ctgaaacgtt ttttcagccg caaaggaatt attctggtgc acggttatat ggcggcgccg
     9241 gaagaaatca ggccgctggc ggactatctg catcagaacg gctannnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnntggcgg cgccggaaga aatcaggccg ctggcggact
     9421 atctgcatca gaacggctat aacgtttacg gcgtgcgcct caggggccac ggcacggcgc
     9481 ccgaggatct cgccatccgg aactgggaga aatggtacga ctccgcaagc cgcgcataca
     9541 tcatcatgaa aaatagcgtc aaaacgtttt caatcgccgg gttttccatg ggcggcggca
     9601 ttgcgctttt acaggcggcc aataaaccgg gccggtttgc atcggtcata tcgatcaacg
     9661 ccccgatggt attaagaggg atggcgtcaa aattcgcgcc ggccgtcgtc gcctggaata
     9721 cactgctcaa aaaaatcaac gttgacaagt ggaagatgga attcattaaa aatgatccgg
     9781 aaaatccgga cattaattac cttcgcaatc ccgtcagcgg aagttatgag ctggaaaaac
     9841 tgatgaaaag ggtcgatcat catctgcaaa atatagccga tccggcgctg atcattcagg
     9901 cgtcggacga tccggttgta gatccctgca gcggccggga aatcttcaac aaaatcgaca
     9961 gcgggaataa aaagtttttc atggtcaacg ctaaccatca tggtatactg cgcggcaaag
    10021 aggcaaccga agtcaatgcc aaggtcctgg cttttttaag tgaatccgct tcaaaataaa
    10081 ttattgatcc tggcgaattc acgaaagtga acgtggcatt ctttacaggg gccgcccgta
    10141 aaaacagcaa aataattaag gggaacatta tgattgaaca atggatgaaa gaaataaaaa
    10201 gaacggctcc ggccaacagg ttggggatga ttctttccca taacggcatt gtccgggcga
    10261 catcaaaaac cggcgaatcg gttcaggcga tgcacctttc ttatgataag acaaagctgg
    10321 aaaaagcggt gcgcgaattt aaaggaaagg aaggcatcgt cgatatccgg gtttggatca
    10381 atgaaggtca cctgaagatc ggcgacgaca ttatgaatgt ttgcgtggcc gggcgcttcc
    10441 gcaaagacgt tttgcctgtt tttcaggaac tgatttccat gattaaaacg gaaatcgtta
    10501 aagaagaaga gatttaacaa aaagttgatc tggaatggcg ccgtgcaccg cggccggttc
    10561 aagccacgga tggggtgatg attcaataga accattgcca tttattgggc gatgtcatag
    10621 accgttgcat agcttctctt gttcggcaac ctttcttttg tagtaatcac acgtcgaatt
    10681 ggccttaaag gcatcgacat tactttgtac gtgatcctgg aactgcttgc ccgttttttc
    10741 cgatactctc gcctgatttg tctgcggaag attcggagca gcgctgactt ttgcagaagc
    10801 agcctgccag caataaatat ccatactgtc ctttttcatc ttcagaattc tctggcattc
    10861 cgcaatcttt ttctcccgat cattcttcgg gtcttcgctt ttctgcccgg atttttttga
    10921 cactttattc tttgccggct gttcagaatc atcggcctca ttcgcatcat cctctgccat
    10981 gttttcttcc ggaatatcct cttcgatatt atcctcttcg attttagcga ttcccgaacg
    11041 gctgaaactg accaggcccc catatttgtt aaaaacgata ttatcgccat ccatttcgta
    11101 gttgctgacg gaaatggttt ctccgttttt caatataatc cggtagtcgg cgtacgccgg
    11161 aacagtcaaa aaagcggctc cccaaaaaca aattgcagaa accatgacga cggcatgtct
    11221 gatcattcgc gatgtctccc gtaaaatgaa aggaatgctg tgctgatttt tatctgtcct
    11281 cttcctttat ttttaatatt attttgcaaa cagtgtttaa tgatcccttt tagtcaaacc
    11341 aacttttgtc gcggcgatac cagaaatccg gaagaatacg gtacattagc cgggaaaaag
    11401 catcgatgta ttctttgttt ctcttgtcat actgacaggc ctggatgatc aaatctctta
    11461 tcgcgccttt ttcaccggct tcattaaata tgtgatacgc gcaggagaaa agcgggctgt
    11521 acatattctt cttttccaga tatttgtgca cgtttctgac ggtattgggg ccttcgggcg
    11581 ttccatcgat tttctccaga acgcgcagat tggtttcgat gggattcccg gtataaagtt
    11641 cataaaaata ctttccataa cggtaattct tgctcgccag cgaggaaacc gttacataca
    11701 tgtcccccac gcccgcctga ctgtgaaaag cctgaaagct tcctcccatc aggcgggaca
    11761 acgaacgaat ctccacaccg gaccgggcaa agatggttcc ggggatggag tccccaagtt
    11821 gcagggaatc cgccagtccc ttgatattgg ccacgacgtt tttcaaagca ccgcaggctt
    11881 ccacgccgac aatgtcaaag ttgtagtagg aattgatttc cttgcggttg aatttcagca
    11941 attcttctct gatgatattg gcgatgcgct tcttgccggc aaccgtcaga cacaccggat
    12001 tgcccagcgc gatatccatg tcaaaaaacg gtccgccgat gcatccgatc tgatatttgt
    12061 gcttcaggtt atacaagctg ttattgatca gttgcgacgg cgtgatcagt tctttggaaa
    12121 tttcatcgga aatcaatcct ttaatcggag aaatcaggca ggtatcgccc gcttttttat
    12181 caatcatcgg cttgagataa ttgagaaatt ccggcaagcg gttcatcgtg atcgtcagaa
    12241 taataaaatc gttattttca acaatatgtt ccagatcatt tgtggcatac accttgtccg
    12301 caagacgggg aaccttctcc attccgccca gacggccggc cagatcttcc gtcaaatgca
    12361 gaggatttaa atgatcccgg ttgatcgcct tgcagacttc cgcatcatgg taataaatgg
    12421 caacccgttt attatcacga ccgaatttat aggcaaaagc tgtgcccaga cggccgggac
    12481 cgataatagc aagccttgtg gtatctaaat cagacgtcag catcaatcaa tctcgttgtg
    12541 aaaaaattcg tctttatggc aatgcaaaac acattaccgg tttttaatat cttcttttaa
    12601 aaaaaaatta aactaaattt attaatgagc actgattccc tacccatgcg tttcggggaa
    12661 atcaagagta ttttcgatat tctccgctaa tgcgggatag ttcaactaat aaaccttgct
    12721 gcgctctgcc tgcaatgctt gagtttcact aattcgacca acgactcaaa cgttcttcac
    12781 tattcattag cccgctccgc tgcgcttcgg ggataattcg actaacgttt cgaacgtcgt
    12841 tgcactcgtt ctcagcgagt ctcattattt ttgttgcatt atcataaatg attgtttaaa
    12901 aaaagcaatg ggttgatttc agaagcatgg cagaggacca cgaagcaaca cggcaagaat
    12961 attttcgtaa aacgatcttc agaattttaa acttgcattc atccaattga aggcaaaaaa
    13021 gagattatat tttaatggaa aaacaaacac ttggtaaaga aatcacgcag gaatttcacg
    13081 acgggaaaat taaagaaaag aaatatgtgc ggcaaggcaa aaccgaaggc gaatattttt
    13141 ctttctacca tagtggccaa attctggccc atgtcaattt catccgcggg aaaaaaaacg
    13201 gccccgcggt catttattat cacgacgggc atatcagaga gaaggcaacc tttgtcaacg
    13261 accggatgga aggcgaatac gtttcttact tcgaaaacgg ccacatccgg gagcatgagt
    13321 tttacagaaa cggcaagctg gatggaaaat atgagattta ttataaaacc ggccagttaa
    13381 agctggtcga aacttttaga aacggcaagt ttgaaggtga atatatcgcc tattacaaaa
    13441 acggacaaat caaggaaaaa ggatttttta aaaacgacaa acgggaaggt gaatatatcg
    13501 cctactacaa aagcggacaa atccgggaaa aagccactta caaaaacggc aactgcgtgg
    13561 ggaaatttct ggcctttgag gaagaaatat ccggttccgg ggaaacaaac ggcagcgaga
    13621 gtgacaccga aatctgggat gaaaaagatg ttgccaattt gctggaagag ctgaaacatt
    13681 ttgataaaaa acaaaagtga atgttttatc gccaggaggc gtcattatga tcagtaacat
    13741 tttaattcct acggacggtt ccgcaaacag ccagacggct ttggattacg gaatctatat
    13801 tgcccgtaaa cttgccgcct ctctgacagg cctttatgtc atcgatgtca atctgattca
    13861 ggggcctatg gtgacggata tttccggttc gatcggcatg ccgccctatg aaggattctt
    13921 cgaatccata gaaaaatcgc tcaatgaaaa agccgatttc attctgaagg aatttgagga
    13981 acgctgccgg aaatccggtg ttaaggccgc cacggacagg aaaaccggca aaatcagccc
    14041 gattattatc gaagaagctc aaaaggccga tctgattctg atggcaaaaa agggagaaca
    14101 ttttcatctg aaggaaggcg gtctgctggg gtcggttgcc gagtcggtaa cacgcaattc
    14161 cggaaagccg gtattggtta ctccggaaaa atttgtagaa atagaaagca tggcactggc
    14221 ttttgacggc agtgagtcgg caacaaaagc cctgggtctt tctttgaatt tatcccacaa
    14281 aacctcctgg ccactgacgg tggtgattgt cagcccggac gagaaaaaag ccgaggcgtt
    14341 atccggaaag gtcgataaaa tgattcagca gaatccggat gaggcctcta ttgactgcga
    14401 aacgatcatc ctgtccggga aagaacagga tgaaatcgtc aaattcatcc gggaaggctc
    14461 tgtggaatta atggtcatgg gagcttacgg tcacaatcgt ctgcgcgaat tgctgctcgg
    14521 cagtaccaca tcgcacgtta tccacaaaag tccgattccc gtattgttga tccggtaacc
    14581 tgaagtttgt tcaatgtccg agtcgacaat accggcaatt gaatagaaaa aaatggaaca
    14641 tcgttacggc aaaaacatcc ttccgctgtc gacattcgat aatgaaaatt tcatcgggtc
    14701 accccccctg gaaattttat ttttttataa aaaagatctt cccatattaa agctcaaaga
    14761 gagtctgtta aagacgatcc attattacaa tcttttttcc agtcgactga tcatgatcgg
    14821 cgatcataaa tttgcgcttc tgtattgcac ggatggagcc gttgcaaatg atctgcggcc
    14881 tgtcgacgcc tgttttgatg acagccgcat tgccgagatc aggaaagaaa tggtgcatgt
    14941 tcaaaccttg ccgggcgaac ctctctttgc tgtaaccggt attcctttca aaaacgggat
    15001 actggccggc atcagttgct ctcacgcggt ggcggatggt atttccctgc tgctttttct
    15061 ttacgcctgg atgtgcataa cggacgggaa aaattttctt ccaccgtcgc cccagagatt
    15121 gtttcaaggc ccgctcgccc atttcgatcc aacagacaga gcgcttgttc cccccttatc
    15181 cgcgctgaac gatcaaatcc gggaaaaagt agacatccat aacgtcgcag agattttcac
    15241 cgccacagaa tactttaccg atgattttct caatgcaatt aaagcggaag cagaaaaaga
    15301 aaattacgac atttccaacc atcagatcat gacggcgttt ttattaaaaa aatatcactg
    15361 taaaatcctg ccggacaccg accggatcgt tttaaaaaat cccatcaatc tcagagacat
    15421 ccatccggat attgatccga tgtatatcgg cagttcaaat ttcaacaact tcatacagtt
    15481 caccaaagaa gaaatcgaca ggctttcgat atctcaaatc gcgtatcgca ttaaaaaatc
    15541 aatcgctgct gtgcgcaatg aaagctatgc gcaggaaata gcacatttat ccccgtacgg
    15601 cattgaaatc aatatggatg tcttcagaag aaaccgcccg ccctacagtg tggaaacgaa
    15661 tgtcgtttcc agcaacctga cgcatctgaa cgatcttgaa tccatgtttg ttggaccaga
    15721 aaccggaagc ctgttgcata tcggcctggc agtccaaacc ggatttacca tgctgaaaga
    15781 aaagagcggg cggatctttg ctcagataac cggcaggcat ccattaacct gatgccgata
    15841 acctttacgg gcggtgacgc gatgtccgtc atcgctggct cggcaatcag ccaatgtctt
    15901 aaaacctgtt tttctccagg gcatctttta actgatcgag caggtcatgt ctggtcaaat
    15961 caaaatggat cggtgtgact gtaatgaatc cggacgtcag cgcgccgacg tcggtatctt
    16021 tctgccgggt ggcggaagac gactccccgg caaaccagta ataggtattt tcccgcgggt
    16081 ccgtccgttt ttcgaaatac tccacgatat tggtctgcgc ctgacggaca accacaacgc
    16141 ccttgatctt tccctgggga aggcagggca cgttgacatt aatcgcgctg cccagaagtt
    16201 gttcccggtg attgagaatc atgcgggtca ttttacgcgc aaatttggcg gcaaacgtaa
    16261 aatccgcttc tttgtgcgta tccagagaca ccgccatgga cgggaatccc ataatcgccg
    16321 cttcggttgc cgcggaaacc gttcctgaat acagcacgtt gatcccggcg tttcctccgc
    16381 gattgattcc cgaaacaacc agatcgacgg gttgatccga taattcttta atgcccagtt
    16441 tcacgcaatc ggccggggtt ccgcatacgg cataccccaa aaattgattg ttcttggtcg
    16501 cacggcgcac catcagggga cgcgaaatcg taatggcatg tccgaccgcg ctttgctccg
    16561 tctccggagc gacaatcaga caatcggctt ctttggaaag ttccttataa agggcgctca
    16621 atcccttcgc gtagattcca tcatcgttgg tcaacaaaaa acgcattatt tttcatcctc
    16681 tccgatcttg tattttcagc cggcaacggg aacagactgc ctgcacgctt catgttcctt
    16741 ctttcataat gcccagaata taattacggg catttagcgc cagatctcgt ccttccagca
    16801 ggacggaaat ttcattatta tatttaagcg cggattgcgt gaaattatgg ctgcccagga
    16861 aaatcagacg ctgatcgatc accgtcagtt tcgtatgcgt cgttttgcgc ggcgaatcaa
    16921 aataaacccg gatgcccttt ccttcaagaa acctgcccgc cttcctgttt tgaatattca
    16981 acgcatccga cggatcacct gtgttttcca gaaccacaaa aaccttgact ccccgttttg
    17041 ccgcactggc cagatggccg gcgatacggt caggataact gttcttgtgt actcccgcct
    17101 taaaagaaaa aatggacatg ataatctcat cctgcgctcc atcaatggca ttcaacaatg
    17161 cgggcagaaa ttcctcattg gtcagaagga ccgttttaca ttgattcgcc tgccgttctt
    17221 tgcctgcggc aaaaacaccg gtggcaaatc ccagaatcag aaaaaccaat accggcaggt
    17281 aaaatatttt ttttctgaac ggcaacatat tcattgtcct ctcccattct tgctattgta
    17341 tattatttga gcattccgcg cgatataaaa aagcccgaaa cggcagca
//
LOCUS       DAONQZ010000048        16981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_3789_length_16981_cov_18.276202, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000048 DAONQZ010000000
VERSION     DAONQZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_16981_cov_18.276202"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLR13_08285"
     CDS             complement(<1..804)
                     /locus_tag="PLR13_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19374.1"
                     /translation="MAQKKDPLYLTWMGTAGVLIEDDHDGILIDPYISRPGLLSVVLG
                     LSLKPDRDAIEKWLTRPGTKNIRAVLVSHSHFDHCLDAPFFALKTNALFIGSKSALNA
                     ARGVPLDESGLKEAGIGRDIKIGAFTLRFIESAHGPAFLGRVPYAGTIDRPLTGRHSA
                     RDYKLGQTYSILITHASGTILHHGSAGFINGMYDGITSDIVLLGIGGRGHTQTYLENV
                     PLKLNAKIVIPIHFDNFFVSLDRKMKNLPGVNFQEFLTTAGKYGNRFQLK"
     gene            complement(853..1617)
                     /locus_tag="PLR13_08290"
     CDS             complement(853..1617)
                     /locus_tag="PLR13_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQC19375.1"
                     /translation="MTNQQAHQKISTALMRDIFFHKLQPGEKLPSERELSGSLNVDRT
                     SLRVALKQLEAMGLLDIRRGDGIYVKDFLKHAGIDFFRTLFSRIETLEDELAADLFVI
                     DEVWEWWIIMFPEMLKLAAQRASARDLKALSDLMDSELNNLDHRDVLIELWLDEQDMI
                     ARAVNNMVLILIFNSSRPLRRSMLRIYHDAVNRENMEQFIISKKSLLMDFLMNSDKTM
                     LADVEQYRDILIAQRQVARSRLIQSGPSPGKTGKEK"
     gene            1915..3318
                     /locus_tag="PLR13_08295"
     CDS             1915..3318
                     /locus_tag="PLR13_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC19376.1"
                     /translation="MRDVYVIGAHTIKFGKYIEKSIKDLAAQTVISCLKEAHLEKKDI
                     QALWFANSGWGYSKGQDCIRAQVALRPIGIDAIPMTNVENACAGGSTAFHHAWLGIAG
                     GLYDITMAVGAEKLYDNNKMKVFAGFLSGLDIENLSSIIANISFYGMSEQDKKDMQAH
                     VAKYAALAKKNAAIKRKKKGVGSRFSDLLDMFKVYLRLSDAVGSETVRKMRKLFSGDH
                     SPFMDVYGFAARQHMKKYGSTIEQLALIASKNHFHSSLNPNAQYTFTITPEEVLADRM
                     VSWPLTRSMCAPIGDGAASAVLCDQATVKKLGLSSQAVKIRASVLGSGRERSFDDTDI
                     GERLSKIAYEKSGLGPGDMDFAEVHDASAFGELHQAEALGFCGEGEGGRLAESGATRL
                     GGKIPVNVSGGLESRGHPVGASGLAQIHELVTQLCGKAGKRQVEGAKIAIAENGGGAL
                     GQEEAAMCIHILEGPSK"
     gene            3372..>4138
                     /locus_tag="PLR13_08300"
     CDS             3372..>4138
                     /locus_tag="PLR13_08300"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC19377.1"
                     /translation="MDLNKKFPEKRVVITGAGSGLGRALALEFARRNWRIAIAEINDE
                     RAAESAKMVREQGGIPLTIHCDVTLPRDMENVLTIVRREWKGVDILINNAGVASAGWF
                     ERIPLEKWDWIIGINLMSIIYGCRTFIRYFKESGTGGYIVNVASSAGIASLPEMVSYN
                     TTKAAVISLSETLRIELSKINVGVSVVCPTFFKTNLMDQFSSPDERQRQLADTFFGNA
                     KITSEQVARHTVASIEKNRFYVITQADGRNMWRMKRW"
     assembly_gap    4139..4238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4729..6411
                     /locus_tag="PLR13_08305"
     CDS             4729..6411
                     /locus_tag="PLR13_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQC19378.1"
                     /translation="MGIAGDIVIIVMAALLGGLIAQMLKQPLILGYILAGVVVGPYTG
                     GVTISDIHSIEKLAEIGVALLLFALGLEFSLRELRPVRAIALFGTPVQIILTTLYGFG
                     IGRILGWSWTSSVWFGALISLSSTMVLIKTLEKQGMMGTLSSRVMIGVLIVQDLAVVP
                     MLIILPQLNNVQDGMAILLVAAVKAAIFLTVMILVGTKIIPRLMRYIANWNSRELFLI
                     TTTTIGLGIGYATYIFGLSFAFGAFVAGMLLSQSDYGYQALSDIIPLRDVFSLMFFTT
                     IGMLLDIRFLLANWEIILLLVGLVMLGKAVILAVLSRVFGYHNVIPLAMGLGLSQVGE
                     FSFILGRVGISTKSITGEFYTMVLTMTIVTMLLTPFISGLTTPLYAMARRWMKPLKLE
                     PLNFPSKGLKDHIVIAGGGRVGMYVANVLQQKNIPFVILEYNSRRVHEVRKSGFPIIF
                     GDAAKTIILEAADLKRAKLLLITTPVAVISLGIVAHAQRINPGIRIIARAEGEEQMKA
                     LYTKGVTYVVQPEFEASLEIVNQTLLNLGIPAEQFKTFTEDARQELHRPLSV"
     gene            6519..8312
                     /locus_tag="PLR13_08310"
     CDS             6519..8312
                     /locus_tag="PLR13_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC19379.1"
                     /translation="MTKATLWWMWPRRHRVFFIFEGREFTYGDVYRQAQRYAHFFLSI
                     RKKLVDSGKLASDQRMAFGVYMENTPEFLFASFGAALSNSVLFAINTGFRGDILGKVM
                     EQAGISFLIVNDSTLKEVEGVVKNVNIIGPENIYVVREGEKSLPDGFQWLDKAVEEAQ
                     QIDVPNRYRVRVDNFSPVIVIYTSGTSGMPKGVPCTHIKMFGAGFVVQSAVHLNRRDR
                     GYISMPLFHSNAWYIGILPQMIAGGSFVLKRRFSASAFEDDMLAHGVTFLNYVGQPLH
                     YIIDALERKYGSGEAVEAALAKHPRNRFRKAYGNGATVNDRIKLKRYLGMDHIYEIYG
                     STEAVITTANCPGDPVDSVGRVDGSVRILDEEGKECEPGMVDEKGALLNYDKAVGEIC
                     RKVGDNNLRFDGYYGNAGATSQKFRNGWYHSGDLGHIRIIKGKRYMFFNGRTDDWIRK
                     DGENFSAENVLDFAQKIPCVDCAIAYGAPCEVADEKVMITVQLKEGADFNPDEVHEWF
                     IQQQKEGGMDPKWMPDYIRVIEKFSVTDTQKILVRPFKRENFNIEINASMRIYFRRRG
                     DTAYRLLTPDEFRKIREDFKTNGREAILASC"
     gene            8380..9018
                     /locus_tag="PLR13_08315"
     CDS             8380..9018
                     /locus_tag="PLR13_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQC19380.1"
                     /translation="MLQKIIIATIIFLFSLSLCWAAEGIKLPAPQMDGGKNLMNAFKE
                     RKSERSYSAKKIPLNVLSNMLWAACGINRPEAGKRTAPSAMNRQQIDVYVATADGLFL
                     YEAKGHLLKPILNQDIRPLTGKQSFVAQTPVTLIYVADYKKMGNISNEEKAFYAATDT
                     GFISENVYLFCASEGLGTVVRGSIDKEALAKTMKLGDHQKIVLAQSVGYPKE"
     gene            complement(9190..9450)
                     /locus_tag="PLR13_08320"
     CDS             complement(9190..9450)
                     /locus_tag="PLR13_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQC19381.1"
                     /translation="MEETENKLHLEYPCAWIYKIIGTDEDEMRMAIDEIIQDRNCLVT
                     FSRSSRNNSYRCLNVEVIVESESHRQTLYELLKAHRAIKIIL"
     gene            9980..11896
                     /locus_tag="PLR13_08325"
     CDS             9980..11896
                     /locus_tag="PLR13_08325"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQC19382.1"
                     /translation="MKSFLANHYAIKDLNAEINNQVVDQMIKRLDPSKTLLYDSDVQN
                     LRPVLRRAFSSMQSGDCALLKPVYDLLVTRARENEKIIRRILGSNYRIDEDAELYIDV
                     KKRPYVKTAAEKYDLLKKVVQFQIENDLLSGVTLAEAKKQQVHRYELQTARVLERRPE
                     KLIEYAAEAFAQALDPHTNYLSPDNLEDLKIHMQLSLEGIGALLSSDNGFTVIEELIP
                     GGSAEKSGLLKPKDKIIAVAQKRAKPVNVIDMDLRDVVKMIRGKKGTQVTLMILRQSE
                     RTYRFDITLTRDKVYIKDQEAKVDYQTRMVNGKKYSFAVIDLPSFYGDEKENKSSYED
                     VRALLMEARQRGVDGVVLDLSRNGGGLIGEAVQIAGLFLGRGPVVATKDNSEKLTVFV
                     NGAATSEMKLRKNKIITFPAEDRPLYTGPLVVLTSRLSASASEIVAGALQDYRRAIIV
                     GSDRTFGKGSVQTLAPLPRDLGGMKVTTGLYFLPGGKSTQKTGVPVDVRLPGFFVLED
                     VGEAELEYPLPAQAIQPFIKASSNSALLWTTVDHPMVAKLSIKSQARIAKDPKFKEII
                     QKNKEAAEKKGLIRLVDLREERKKEGGSNKKETPAELKQKVRDQYAPYVNESINILSD
                     MMTAKAALPMAKAL"
     gene            complement(12101..13132)
                     /locus_tag="PLR13_08330"
     CDS             complement(12101..13132)
                     /locus_tag="PLR13_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19383.1"
                     /translation="MPGRIPLTMDCQPSFFSAIDVEGYVHRTAVAENNHRIVGVVSMS
                     KRRLYLNGVPEDFGYIGGLRIDSSLRNTTILVRLHNILKKWHQEGFGVRFYLSAIPYD
                     NVGARNIMTSGRAGFPDCTDMGVLYNAAIPLFRRRLPRPQKGLRIVRGTAVGADAIAE
                     FLNRNGSRRQFFPVYTADDITADNGILRGLHPDDFYVAVERNKISGVMACWDQLPFRR
                     MLIADYPWHLHWFKRLSAPLAGILHTAPMPEPGEPLRNIVAACIAIENNHPKIFNLLL
                     QTILHDHYDTGKAFLVVGLMEKDPLISALKSRLHISTRTCIYMMSGNGYNILSEMDER
                     PPYLELGAL"
     gene            complement(13350..13853)
                     /locus_tag="PLR13_08335"
     CDS             complement(13350..13853)
                     /locus_tag="PLR13_08335"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HQC19384.1"
                     /translation="MSWYVIHTKSRHEHKVNDGLVQKNLTTFLPEVEVWSKRKDRKKK
                     IMVPLFSSYLFVDTDLNNYETRLDILKTPGVVRILGKKENSEPVAVPDDAIRAIQKFL
                     NKKTEIFSLQYPQKGEWARITGGPFAGLEGIVVKSDLNKEMFVVSIDLLHRSVAVKVE
                     GFKITRL"
     gene            14144..15364
                     /locus_tag="PLR13_08340"
     CDS             14144..15364
                     /locus_tag="PLR13_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19385.1"
                     /translation="MEKTEYLKINLGKNILPLSAAENKYMPGAGPIEIIISYKRSTAV
                     ARGIECFLKAADYYNLFSSRLIMIGENTFALQYCTDGIVINILPPVHARFDDVRIDDL
                     REKMTHVQTLPGHPLLALTLIPVNDGHFVGFSCSHAVADGISCILFLYAWNCLIEGKP
                     FPRPSAQRLFSGNPIPSDHIEKDWMPPLSALSEEIQNRVRRGAIKTYKTTEYFADEFL
                     AEMKHQAQANNREFVISNNQIMTAWLLKKYHHHILPHTDKIVIRTPVDLRTVHPGVDP
                     MYIGYAVINSFSEFRKKEIDHMSVVETACRLKESIHHTRTKRFIQEATYLSKYGIEIR
                     EEVLKQFPPYNPDTDIVSTNMTHLSDLESLFLGPDAMRIEHIGVASVKTSFAMVREKN
                     SQMSAQITSRYPLI"
     gene            15386..16705
                     /locus_tag="PLR13_08345"
     CDS             15386..16705
                     /locus_tag="PLR13_08345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19386.1"
                     /translation="MLSSASRYNIRFARPEEIRDIIELMRPYNMHHIPSPEMPPLDDK
                     RFIVVEQNGQLLGAAGYTFVSEEIGKTTLMAVRPDCARMGLGKILQTRRMQILRGLGC
                     TKIITNTDRPETIAWYKQHFGYREIGKLPKIHSFSLEHVPEWTTLESDLTTIDVPSPV
                     QGKLLMNAALTGMKHTRSDNPYLPVTPAEIAEAALEAGKLGAAIVHLHARDVDEKPTP
                     DPSIYAEIISRIREKNADLIICVTTSGRNWSDLQKRSAVLELPDDVKPDMASLSLGSM
                     NFPSGASINPPEIIRSLALRMMERNIKPELEAFEVGMVEYAKYLIREGVLRQPVYLNL
                     ILGSLGTMSASPDNLRYLVSRLPAFSTWAASGIGRYHSKVQDMAAAMGGGIRTGLEDS
                     SMHLDADKRIPAKNKDWIKRAVEAASLNGREIATPNDVRQWLDLRKS"
BASE COUNT         4301 a         3956 c         4456 g         4168 t
ORIGIN      
        1 cttcaactgg aaacgatttc catatttgcc ggctgtggtc agaaattctt gaaaatttac
       61 gccgggcaga tttttcattt ttctgtccag ggacacaaaa aaattatcaa agtgaatcgg
      121 aataacgatt ttggcgttta gtttcagagg aacgttttcg agatatgttt gggtatgtcc
      181 cctgccgccg atacccagta aaacaatatc cgatgtgatg ccgtcataca ttccgttaat
      241 aaatccggcg ctgccgtgat gcaggattgt cccggaagca tgtgtgatca gaatggaata
      301 cgtctgtccc agtttgtaat ctcttgcgga atgccggccg gtcagcggcc ggtcgattgt
      361 cccagcgtag gggactcttc ccagaaaagc aggtccgtga gcgctttcaa taaatctgag
      421 cgtgaaagct ccgattttga tgtcccggcc gatgcccgct tccttcaaac cgctttcgtc
      481 cagggggaca ccgcgcgccg cgttcagtgc gctttttgaa ccgataaaca gggcgtttgt
      541 tttaagcgca aaaaaaggag cgtcaaggca gtgatcgaag tgggagtggc tgacaagaac
      601 agcgcgaatg ttttttgttc ccggacgggt cagccatttt tcgatagcgt cccgatcagg
      661 tttcagcgaa agtcccagaa ccacgctcag cagtcccggg cggctaatgt aggggtcgat
      721 caaaataccg tcatgatcgt cctcgatcaa tacgccggca gtgcccatcc aggtaagata
      781 caaggggtct tttttctgcg ccacgccgtg gccctccccg aaaggttcga tatttttatg
      841 gagatgcccc gattattttt ctttgcccgt tttgccgggg gatgggccgc tctgaatcag
      901 tctgctccgg gcaacctgcc gttgcgcaat cagaatgtcg cggtactgtt ccacgtccgc
      961 gagcatggtt ttatcgctgt tcataagaaa atccatcagc agcgattttt tgctgataat
     1021 gaactgttcc atattttccc gattgacggc gtcatggtaa atgcggagca tacttctgcg
     1081 caacggccgc gatgagttga aaatcagtat cagcaccata ttgttgacag ccctggcgat
     1141 catatcctgt tcgtccagcc agagttcaat gaggacgtcc cggtgatcca ggttgttcag
     1201 ttccgaatcc atcagatcgg acagcgcctt gagatcgcgc gcggaagccc tctgggcggc
     1261 cagtttcagc atttccggaa acatgatgat ccaccactcc cagacttcat cgatgacaaa
     1321 cagatcggcc gccagttcgt cttcgagggt ttctatcctg gaaaaaagcg ttcgaaagaa
     1381 atcaatcccg gcgtgtttta agaaatcttt tacataaatt ccgtctccgc ggcgaatgtc
     1441 cagcaatccc attgcctcca gttgtttcaa ggccacgcgg agtgatgttc tgtccacatt
     1501 cagggagccg gacagttcac gctccgacgg aagtttttcg ccgggctgca gtttatgaaa
     1561 aaatatgtcg cgcatcagcg cagtcgaaat cttctgatgt gcctgttgat tggtcatgtt
     1621 tcgtacgaac ctcccgaata ttttattgct ttctttcaat ttctatcaaa ctgacctgat
     1681 gcattacatg cagtcatgtc ggtaagttat cctaagatgc ccatctcaaa agtgaatcac
     1741 gtttgttgcc ggacgatgtc cgtgagattc aagaatgggt tctccgatgc gtcattgaac
     1801 taaacgtatt tattatgtat ttttttcttg acaaaatcaa gaccaaaata tatacattgg
     1861 ttaaaccaat cagaacaata gatagtaccg cactgaaagt tgaggtccat tgtaatgcga
     1921 gatgtttatg ttatcggagc tcataccatc aaattcggaa aatatattga gaagagcatt
     1981 aaggatcttg ccgcccagac ggttatttcg tgtctgaaag aagctcatct ggagaaaaaa
     2041 gacatacagg cgctgtggtt tgccaattcc ggttggggat acagcaaggg acaggactgc
     2101 atccgggcgc aagttgcgct caggccgatc gggattgacg ccattccgat gaccaatgtc
     2161 gaaaatgcct gcgccggagg atcgacggct ttccatcatg cctggttggg cattgccggc
     2221 ggtctttatg atattaccat ggccgtaggc gcggagaaac tttacgacaa caataaaatg
     2281 aaagtattcg ccggtttttt aagcggtctg gatattgaaa acctttccag cattattgcc
     2341 aacatttctt tttacggcat gtcggaacag gataaaaaag acatgcaggc ccatgtcgca
     2401 aagtatgcag cgctggccaa aaaaaacgcc gcaataaaaa ggaaaaaaaa gggcgtcggc
     2461 agtcgtttca gcgatctgct ggatatgttc aaagtctacc tgcgcctgag cgacgccgtc
     2521 ggttcggaaa ccgtcaggaa gatgagaaaa ttattttcag gcgatcattc gccctttatg
     2581 gacgtgtacg gatttgccgc tcgtcagcat atgaaaaagt acggttcgac catcgaacag
     2641 cttgcgctta ttgcatcaaa gaatcatttt cattcttcgc tcaaccccaa cgcgcaatac
     2701 accttcacga taacgcccga ggaggttctg gcggatcgca tggtcagctg gccgctgacc
     2761 cggtcgatgt gcgcgccaat cggcgacgga gcggcgtctg ccgttttatg cgaccaagca
     2821 accgtgaaaa aactggggtt gtcgtcgcag gcggtcaaaa tcagggcatc cgtgctcggt
     2881 tccggacgcg agcgctcttt tgatgatacc gatatcggtg aacgtttaag caaaatcgcc
     2941 tatgaaaaat cggggctggg acccggggat atggattttg ccgaggttca cgacgccagc
     3001 gccttcggtg agctgcatca ggcggaagcg ctgggtttct gcggtgaggg cgaaggcgga
     3061 cgtttggcgg aaagcggcgc cacccgactc ggcggcaaga ttcccgtcaa tgtcagcggc
     3121 ggtctggagt cgcggggcca tcccgtcgga gcatccggac tggcgcagat tcacgaactg
     3181 gttacccagc tttgcggcaa agccggtaaa cgacaggtcg agggcgcaaa aatcgccatc
     3241 gctgaaaacg gcggaggagc cctgggacag gaagaggccg ccatgtgcat tcacatcctg
     3301 gaggggccgt caaaataatt tgcggcgggt atgtcccggt atctcaaaat taaaaacaga
     3361 aggtgaacac gatggatctg aataaaaaat ttcctgaaaa aagagtggtg attacgggtg
     3421 cgggtagcgg tttggggcgc gctctggcat tggaatttgc caggagaaac tggcggatcg
     3481 ccatagccga aatcaatgat gagcgtgccg ccgaatccgc aaaaatggtc cgggagcagg
     3541 gcgggattcc gctgacgatt cactgcgatg tgaccctgcc ccgcgacatg gagaacgttt
     3601 taaccatcgt ccggagagaa tggaagggtg tggatattct gattaacaat gccggagtgg
     3661 cttctgccgg atggtttgaa cggattcctc tggaaaaatg ggactggatc atcggcatta
     3721 atctgatgag tatcatttac ggatgccgca cgttcatccg ttatttcaag gaatccggga
     3781 caggcggtta catcgtcaac gtggcgtcga gtgcgggaat cgcctccctg ccagagatgg
     3841 tcagttataa cacgacaaaa gctgcggtca tctcgctttc cgagaccctg cggatcgagc
     3901 tttccaaaat taacgtcggg gtttccgttg tgtgcccgac gtttttcaaa acaaatctga
     3961 tggatcagtt ttcatcgccc gatgaacgtc agcgtcagct ggccgataca tttttcggaa
     4021 acgccaaaat cacgtccgag caggttgccc gacacaccgt tgcgtctatc gagaaaaatc
     4081 gtttttatgt gattacgcag gccgacggca gaaatatgtg gcgcatgaag cgatggttnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tatgtgatta cgcaggccga
     4261 cggcagaaat atgtggcgca tgaagcgatg gttcccggaa ctgtatttca agatattcgg
     4321 cgtcatttac aagaaaggaa tttttgacag acatctcggg aaattattgc gggtggccaa
     4381 tctgttccag taaatgaacg aaatcgtgcg gaaaaatcat gcaaaacttt tccggacagg
     4441 tttcaggccg tcagcgatcg ccacggcatc cgatataatt gattgtcccg caacaccggc
     4501 cctgacggtt atccaaaaat aaaatttatt ttgagccatc ttcatctttt ttagtccttt
     4561 ccggaatttt ttccgtcctc tgaatgatca accagatgaa accgtcttca tacgccattc
     4621 ccgtacatca aaacggattc gatgaatttt tgtaggcgtt ggcagttgaa atgtgataag
     4681 agaaattaat aaatttaaat tcataccttt tatcgggcag tagtttcgat gggaattgcc
     4741 ggagacattg tcatcatcgt catggccgcg ctgctgggcg gcctgatcgc tcagatgctg
     4801 aaacagccat tgatcctggg atatattctt gccggggtcg tcgtggggcc ttataccggc
     4861 ggagtcacca tttccgatat ccacagtatc gaaaaactgg cggaaatcgg tgtcgcgctt
     4921 ctgctgttcg ccctgggact ggaattttcc ttgagagaat taaggccggt gcgcgccatt
     4981 gctcttttcg gcacaccggt gcagattatt ctgacgacgc tttacgggtt tggcatcggt
     5041 cgcatactgg ggtggtcctg gacgtcgtcc gtgtggtttg gagcgcttat ttccctgtcc
     5101 agtaccatgg ttctgatcaa aactcttgag aaacagggga tgatggggac gctgtccagc
     5161 cgcgtgatga tcggcgttct gattgttcag gatctggcgg tcgttcccat gctgattatt
     5221 ctgccgcaat tgaacaatgt tcaggacggc atggcgattc tgcttgttgc cgcggtgaaa
     5281 gcggccatat ttctgaccgt gatgatttta gtcggaacta aaatcatccc ccggctgatg
     5341 cgatacattg ccaactggaa ttcgcgggag cttttcctta tcaccaccac caccatcggg
     5401 ctgggtatcg ggtacgcaac ctatattttc ggtctttctt ttgcgttcgg cgcttttgtg
     5461 gccgggatgc tgttgagcca gtcggattac ggttatcagg cgttgagcga catcattcct
     5521 ctgcgcgacg tattcagtct gatgtttttt acgaccatcg gcatgcttct ggatatccgg
     5581 tttcttctgg ccaactggga aataatcctg ctgctggtcg gactggtgat gctgggcaag
     5641 gcggtcattc tggccgtctt gagtcgggtc ttcggttatc ataacgttat ccctctggcg
     5701 atggggctgg gcctttcgca ggtcggcgag ttttctttta ttctcggacg cgtcggcata
     5761 agtacgaaat ccattacggg cgagttttac acgatggtgc tgaccatgac gattgtcacc
     5821 atgctgctga cgccttttat atcgggatta acaaccccgc tgtacgccat ggcccgccgc
     5881 tggatgaaac cgttgaaact cgaaccgttg aattttccca gcaaaggatt aaaagaccat
     5941 attgtgattg ccggcggcgg acgggtgggc atgtatgtcg ccaatgttct gcagcaaaaa
     6001 aatattcctt ttgtgattct ggagtataat tccaggcgtg ttcacgaagt ccggaaaagc
     6061 gggttcccga ttatcttcgg ggatgccgca aaaacgatta tcctggaagc ggctgatctg
     6121 aaaagggcga agcttctgct gatcacaacg cctgttgcgg tgatttctct gggcattgtt
     6181 gcgcatgcgc aacgcatcaa tccgggtatc cgcattatcg cgcgggcgga aggcgaggaa
     6241 cagatgaagg ccctttatac aaaaggcgtg acatatgtgg tgcagccgga attcgaagcc
     6301 agtctggaaa tcgtgaacca gacgctgctg aacctcggca ttccggcaga gcagtttaaa
     6361 acgtttacgg aggatgcccg gcaggaactg cacaggccgt tatccgtgta ggcctcctgc
     6421 ggggtgtaaa aatttattga tgaagaaagg atgattccat gaaactgaat ctgaagacga
     6481 tcataagaaa atgtcttggt ttcgtgaatt tcagaagcat gacgaaggcg accttatggt
     6541 ggatgtggcc gagacgccat cgcgtttttt ttatttttga aggccgcgaa tttacctacg
     6601 gggatgttta ccggcaggcg cagcgctatg cgcatttttt cctgtcgatc agaaaaaaac
     6661 tggtcgacag cggcaaactg gccagcgatc agagaatggc cttcggggtt tatatggaaa
     6721 atacgccgga atttcttttt gcttcttttg gagcggcctt gagcaactcg gttctttttg
     6781 ccatcaatac cggcttccgg ggagacatcc tgggcaaagt gatggaacag gcgggcatct
     6841 cttttttgat cgtcaacgac agtacgctca aagaggtgga gggtgttgtg aaaaatgtaa
     6901 acatcattgg tccggaaaat atttacgtcg tccgtgaagg agaaaaatct cttcccgacg
     6961 gttttcaatg gctggacaaa gcggtggaag aagctcaaca gatcgatgtg ccgaaccgct
     7021 accgggtcag agtggataat ttcagcccgg taattgttat ttatacatcg ggaacgtcgg
     7081 gcatgccgaa gggggtgccg tgcacgcaca tcaaaatgtt cggagccgga tttgtcgtcc
     7141 agtcggcggt tcatctcaac aggcgcgaca gagggtacat ctcgatgccg ctttttcatt
     7201 ccaatgcctg gtacatcggc atacttcccc agatgattgc gggcggcagt tttgtgctca
     7261 aaagacgttt ttccgcgagc gcttttgaag acgacatgct ggcgcacggg gtgacgttcc
     7321 tcaactacgt cggacagcct ctgcattaca tcatcgacgc gctggagaga aaatacggca
     7381 gcggtgaagc ggtggaagcc gcactggcga aacatccccg caacaggttc cgcaaggcct
     7441 acggcaacgg ggccacggtc aatgaccgca ttaaacttaa acgttatttg ggcatggacc
     7501 atatttatga aatttacggt tccaccgagg cggtgattac cacagccaac tgtcccggcg
     7561 atccggtgga ttccgtcgga cgcgtcgatg gttcggtgcg catcttggat gaagaaggaa
     7621 aggaatgcga gccgggaatg gtagatgaaa aaggcgcact gctcaattat gacaaagcgg
     7681 tgggcgaaat ctgccgcaag gtgggggaca acaatttgag gtttgatgga tattacggca
     7741 acgcgggagc caccagccag aaatttcgca atggctggta tcattccggc gacctcggtc
     7801 atatcagaat catcaagggc aagcggtata tgtttttcaa cggccgtacg gacgactgga
     7861 tccgcaagga cggagaaaat ttctcagcgg agaatgttct tgatttcgcg cagaaaattc
     7921 cctgtgtgga ttgtgccatc gcctacgggg ctccctgtga agttgccgat gaaaaggtga
     7981 tgataaccgt ccagctcaag gaaggggccg atttcaatcc cgacgaggtg catgaatggt
     8041 ttatccaaca gcagaaggag gggggaatgg atcccaagtg gatgcccgat tacatccgcg
     8101 tcatcgaaaa gttttccgta accgatacgc agaagattct tgtgcggccg ttcaagcgtg
     8161 aaaacttcaa tatagagatc aatgcctcaa tgcgcattta ttttcgccgg cgcggcgata
     8221 ccgcctatcg gctgctgacg cctgatgaat tcagaaaaat cagggaggat ttcaaaacca
     8281 acggccgcga agcaatcctg gcgtcgtgct gatggccttg gatctgttgc tttgcgggtg
     8341 tcgatgacat gaatcaggtt tttgagaaag gaggacaaca tgttgcagaa aatcattatc
     8401 gcaacgatca tttttttatt ttcattgtcg ttgtgttggg cggctgaagg gatcaaactt
     8461 cccgctcccc aaatggacgg gggcaaaaat ttgatgaatg ccttcaagga aagaaaatcg
     8521 gaacggtcat acagcgccaa aaaaattcct ctcaatgtgc tttcgaacat gctttgggcc
     8581 gcctgtggaa tcaaccgtcc cgaagcgggc aaacggactg ctccgtccgc catgaaccgt
     8641 cagcagattg acgtttatgt ggcgacggcc gatggacttt ttctttacga ggccaaggga
     8701 catttgctca agcccatcct caaccaggat atccggccgt tgacggggaa acagtcgttt
     8761 gtcgcccaga cgccggtgac cttgatttac gtggccgatt ataagaaaat gggcaacatc
     8821 agcaatgaag aaaaagcgtt ttacgcggcg acggataccg gctttatcag cgaaaacgtt
     8881 tatctgtttt gcgcctccga gggattgggg acggtggtcc gggggtccat cgataaagaa
     8941 gccctggcca aaaccatgaa actcggcgat catcaaaaaa ttgttttggc gcaatccgtc
     9001 ggttatccta aagaataata attcagaacc gcagacaccg atgcggtaag caaaggggaa
     9061 accaaaacgg aagcctgttg actgccgtgg tctgaaggat gcggcgggat cggtcatggc
     9121 gggttgacgg ccggggaggg agtatggccg gacatgacga gacgggttcc tgcggcctgt
     9181 gtcgcggggt tatagaatga tttttatcgc ccggtgagcc ttgagcaatt cgtaaagggt
     9241 ctgccggtgg gattcgcttt ccactatcac ttccacgttg agacagcgat agctgttgtt
     9301 ccgactgctg cgggaaaacg taaccagaca gtttctgtcc tgaataattt catcgatcgc
     9361 cattctcatt tcgtcctcat ccgtcccgat aattttataa atccaggcgc agggatattc
     9421 gaggtgaagt ttgttttctg tttcttccat ttctttcttc gagatttttc gaatttttta
     9481 ggagttatcc ggaaaagctg aaattagtca agcggtttat accatggccc ctctgatgaa
     9541 tcatcggaat catcaacctt tttccggcag aatccggccg gcaaccaagt tttcggcatt
     9601 gctttaggat acatcggggt tgaacggaga cgatcgggca attaacacgg caaaccaaag
     9661 actcccggat ttgatttcat ttttccttga cagaatccct gctccccttt atcgttgttt
     9721 tcacaaacat ttttgtgcaa agaaagcgga tgtagggtac gcggtgaagg aaagcaaagg
     9781 cgtgaaaaga aataatcaaa gcagcggaag ttttttcagc aaaacggcgt cagatcatga
     9841 ccgcgggggg agaaaagatc tcggaaaagg tccccgattg tatctacgaa agtggttttt
     9901 gattctattg ctgttcgttt ttctgcctgt ttcctcctca gttgctgaaa atgtcaagtg
     9961 cagccgcatg cctctcctga tgaaaagttt tctggccaac cattatgcca ttaaggatct
    10021 gaacgcggag atcaacaatc aggtcgtcga tcagatgatc aagcgcctgg acccttcaaa
    10081 aacattactt tacgattccg atgtccagaa cttaaggccg gttctgcgaa gagctttttc
    10141 cagcatgcag tcgggagact gcgccttgct caaaccggtt tacgatcttc tggtgacgcg
    10201 ggcgcgcgaa aacgaaaaaa tcataagaag gattctggga tcgaattacc ggattgacga
    10261 agatgcggaa ttgtatatcg atgtgaaaaa gcgtccgtat gtcaaaaccg cggcggaaaa
    10321 atacgatctt ctaaagaaag ttgtgcagtt ccagatcgaa aacgatttac tgtccggtgt
    10381 gaccctggcg gaagccaaaa aacagcaggt tcaccgctat gagttgcaga cggccagggt
    10441 cctggagcgt cgtcccgaaa agctcattga atatgcggcg gaggcgtttg cacaggctct
    10501 ggatccgcac accaattatc tttctcccga caatctggaa gacctcaaaa ttcatatgca
    10561 gctgtcgctg gagggaatcg gagcgctgct cagcagcgac aacggcttta ccgttatcga
    10621 ggaattgatt ccgggaggaa gcgccgagaa atcaggactt ctcaaaccca aagacaaaat
    10681 cattgctgtg gcacagaaaa gagcaaaacc ggtcaacgtg atcgacatgg acctgcgcga
    10741 tgtcgtaaaa atgattcgcg gcaaaaaagg aacgcaggtg acgctgatga tcctgaggca
    10801 gtccgaacgc acgtatcgtt tcgatatcac gctcacgcgg gacaaggttt acattaagga
    10861 tcaggaagcc aaagtcgatt accagaccag aatggtcaac gggaagaaat acagttttgc
    10921 ggtcattgat ttgccgtctt tctacggtga cgaaaaagag aacaaatcca gctatgaaga
    10981 cgtccgcgcc cttcttatgg aagcccggca gcgcggtgtt gatggggttg tcctggacct
    11041 ttcgcgcaac ggcggaggtt tgatcggcga agccgtgcag attgccgggc tttttcttgg
    11101 cagggggccg gttgtggcga ccaaggataa cagcgaaaaa ctgacggttt ttgtcaacgg
    11161 cgcggcaacg tctgaaatga aattaagaaa aaacaaaatc atcactttcc ccgcggaaga
    11221 tcggccgctg tataccgggc ctctggtggt tttgacaagc cgcctgagtg cttcagccag
    11281 tgaaattgtt gccggagcgc ttcaggacta tcgtcgtgcc atcatcgtcg gatccgatcg
    11341 caccttcggc aagggatcgg tgcagacact tgcgccgctg cccagagacc tcggggggat
    11401 gaaagtgacg acagggcttt attttctgcc gggcgggaag tctacgcaaa agacgggtgt
    11461 gccggttgac gtgcgacttc ccggtttttt tgttctggag gatgttggag aagcggaact
    11521 ggaatacccg ctgccggcgc aggccattca gccgttcatc aaggcatcgt caaattctgc
    11581 tttactctgg acgaccgtcg accatccgat ggttgcaaaa ctgtcgataa aatctcaggc
    11641 ccggattgcc aaggatccaa aatttaaaga gattattcag aaaaacaagg aggctgcgga
    11701 aaagaagggc cttatccgcc ttgttgattt gcgggaggaa cggaaaaaag aaggaggatc
    11761 aaataaaaag gaaacgcccg ccgaactgaa acaaaaagtt cgtgatcagt atgcgccgta
    11821 tgtcaatgaa agtatcaata ttctttccga tatgatgacc gcaaaggcag cgttgccgat
    11881 ggcaaaggct ctatagggta atcggttatc atcaggaagc ggacagaaaa ggagggcaaa
    11941 aggcacagtt tttggcaaac cggtttgcgg aggatcggtg gtggcgcgga aagccggtat
    12001 ctgctgcgag gttgccggaa accgcctgcc accttttaag gagtcgcccg cggcattgtc
    12061 atcgataaga aacgccaggc aaccgtaacg cgtcatgaaa ttataaggcc cccagttcga
    12121 ggtagggcgg tctttcgtcc atttcagata aaatgttgta gccgttccca gacatcatgt
    12181 aaatgcaggt ccgcgtggaa atatgcaaac ggctcttcaa cgctgatatc aggggatctt
    12241 tttccatcaa accgacaacc agaaaagctt tcccggtatc ataatggtca tggagaatgg
    12301 tttgcagcag caggttaaat atcttcggat gattattttc gatggcgatg caggcggcga
    12361 caatattccg gagaggttct ccgggttcag gcatcggcgc cgtgtgtaag atgcctgcca
    12421 agggggcact caaacgttta aaccaatgaa gatgccatgg ataatcggca atcagcatac
    12481 ggcggaaagg gagctgatcc cagcaggcca taacaccgct tattttgttc ctttccacag
    12541 ccacgtaaaa atcgtccgga tgcagccccc gcaaaatgcc gttgtctgca gtaatgtcgt
    12601 cggccgtata aacggggaaa aactgccttc ggcttccgtt acggttgaga aattcagcaa
    12661 tcgcatccgc acccaccgca gtgccgcgca caatccggag gcctttttgc ggccgcggta
    12721 accggcgcct gaataacgga atggcggcat tataaagaac acccatgtcg gtgcaatcag
    12781 gaaaaccggc ccggccgctg gtcatgatat ttctggcgcc gacattgtcg taagggatgg
    12841 cggatagata aaagcggacg ccaaatcctt cctggtgcca cttcttcagg atattgtgaa
    12901 ggcggacgag tatggtggtg tttctcagcg atgaatcgat gcgcagaccg ccgatgtatc
    12961 cgaaatcttc ggggaccccg ttgagataca gcctgcgttt tgacatcgat acgacaccga
    13021 cgatccggtg attgttttcc gcaacggctg tccggtggac gtatccttcc acgtcgatgg
    13081 cggaaaagaa actcggttga cagtccatgg tcagcgggat gcgccccggc atgaaagtcc
    13141 gcttcatcag ggcgcatatg gcttcatcgt cctcccttcg cgcctcccga atcagggttg
    13201 ggggaaaaaa atcaagtgca gtttttttgt ctttgtccgt cattgtcgat tatcaatatt
    13261 taaggaaccg tgaaagcgat atccccctgt tattttttct gtatgatgtg attatctcat
    13321 gaaaaaaact atgctttaaa taaaattgat taaagcctgg ttattttaaa cccttccact
    13381 ttcaccgcga ccgaacggtg taagagatca atggagacga caaacatttc tttgttcagg
    13441 tcgcttttta cgacgattcc ctcaagcccg gcaaaagggc cgccggtaat tcttgcccat
    13501 tccccctttt gcggatactg aagagagaat atttccgttt ttttgttcag aaatttttga
    13561 atcgcacgaa tggcgtcatc aggcactgca acgggttctg aattttcttt tttgcccaga
    13621 atacggacga cgccgggcgt tttcaatata tcaagtctcg tttcgtaatt gttcagatcc
    13681 gtatccacaa aaagatagct ggaaaaaaga ggaaccataa tcttcttttt tctgtctttc
    13741 cttttgctcc agacttccac ttccggcaga aaggtggtca gatttttttg gactaggccg
    13801 tcgttaacct tgtgctcgtg acgacttttt gtgtgtatga cgtaccagga catttttcaa
    13861 tatacgcgat ttttgcactg aaattataac attgccatgt gttttgcaac ggatttatcg
    13921 tattttgatt tttgcaggca cagccatcac agtacggaaa catcagggcc gatcaaatgg
    13981 gataacggac gccccaggcc ggtcggtaaa aaatccgtca cgcccgcatg gatatggccc
    14041 gcttttccct tgacatgcaa aacaatcgcc tatagaaaaa catcatatct ttgttcctgt
    14101 caaaaatgaa gttaccgtgg aaagaacgca taacataagc gatttggaaa aaacagaata
    14161 tctgaaaata aatttgggga aaaatatttt gccgctgtcc gcggcagaaa acaaatacat
    14221 gcccggcgcg ggtcccatcg aaattatcat atcgtataaa agaagcaccg ccgttgcccg
    14281 ggggatcgaa tgtttcttaa aagccgctga ctattacaat cttttttcca gccgtttgat
    14341 tatgattggc gaaaatacat ttgccctgca gtattgtacc gatggaattg tgatcaatat
    14401 tctgcctccc gtccatgcgc gttttgatga cgtaaggatt gatgatttga gagaaaagat
    14461 gacgcatgtt cagaccttgc cgggtcatcc gcttctcgcg cttacactga ttccggtgaa
    14521 cgacggacat tttgtcggat tcagttgctc tcatgcggtc gccgacggca tttcctgcat
    14581 tctcttcctt tatgcctgga actgccttat cgaagggaaa cctttcccgc gtccctctgc
    14641 gcagagattg ttcagcggaa acccaatccc ttctgatcac attgaaaaag attggatgcc
    14701 gcccctgtcc gcgttgagcg aagagattca aaaccgcgtc aggcggggcg cgatcaaaac
    14761 atacaaaacg acggaatatt ttgccgatga atttcttgct gaaatgaaac atcaggcaca
    14821 ggcaaacaac agggaattcg ttatttccaa taatcagatt atgaccgcat ggctgttaaa
    14881 aaaatatcat catcatattc tgcctcatac agataagatc gtcatcagga cgcctgtcga
    14941 tctcagaacc gtccatcctg gcgttgatcc gatgtatatc ggttatgccg tcatcaatag
    15001 cttttcggaa tttcgcaaaa aagagattga tcacatgtcg gttgtggaga cggcctgtcg
    15061 cttaaaagaa tccattcatc acacaagaac gaaacgcttt attcaggagg caacctattt
    15121 gtccaagtac ggcatagaga ttcgagaaga ggttctgaaa caatttcctc cttataatcc
    15181 ggacacagat atcgtttcaa caaatatgac gcatttgagc gatcttgaat ctctgttttt
    15241 aggcccggat gccatgcgta ttgagcatat cggcgtagca tcagtgaaaa caagttttgc
    15301 catggtgaga gaaaagaata gccaaatgtc agcgcaaatc accagccggt atccgctgat
    15361 ataaatcgtc caggaaagtt tacatatgtt gtcatccgcc tcccgttaca acatccgatt
    15421 tgcccgcccc gaagagatcc gcgatatcat tgaactgatg cgcccttata atatgcatca
    15481 cattccttcg ccggagatgc ccccgcttga tgacaagcga ttcattgtgg ttgagcaaaa
    15541 cggacaattg ctcggtgccg ccggttacac gtttgtttca gaggagatcg ggaaaacgac
    15601 tctcatggcc gtacgtcccg actgcgcccg gatggggctg gggaaaatac tgcaaaccag
    15661 acggatgcag attctgcgcg gactcggctg cacaaaaatc attacgaata ccgaccggcc
    15721 ggaaaccatc gcctggtaca aacagcattt cgggtatcgc gaaataggca aattgccgaa
    15781 aattcattcc ttcagcctgg agcatgtgcc ggaatggacc acattggaat ctgatctgac
    15841 aaccattgac gttccgtctc ctgttcaggg gaaattgctg atgaatgctg ccctgacagg
    15901 gatgaagcac acccgaagcg acaatcctta cctgcccgtt acaccggcgg aaatcgctga
    15961 ggcggcattg gaagccggga aactgggagc ggccatcgtt catcttcatg cgagggatgt
    16021 cgatgaaaaa ccgacaccgg atccgtcgat atacgctgaa attatcagcc gtatccggga
    16081 aaaaaatgcc gatctgatca tttgcgtcac cacaagcggt cggaactgga gcgatttgca
    16141 gaaaaggtcc gccgtgctgg agttgccgga tgatgtaaaa ccggatatgg cttctttgtc
    16201 tctggggtcg atgaattttc cctcgggggc cagcattaac ccgccggaga tcatccggtc
    16261 tctggccttg cgcatgatgg aacggaacat caaaccggaa ctggaagcgt tcgaagtggg
    16321 aatggtcgaa tatgccaagt atctgatcag ggagggggtg ttgcgtcagc ctgtctattt
    16381 gaatctgatt ttaggatcgt taggcacgat gagcgcctca ccggataatc tgcgttatct
    16441 tgtttcccgg cttccggcgt tttcgacgtg ggccgcttcc ggaatcggaa gatatcacag
    16501 taaagttcag gatatggcgg ccgcaatggg cggcggtata cgaaccggac tggaagatag
    16561 cagcatgcac ctggatgcgg acaagcgcat accggcaaaa aacaaagact ggatcaagag
    16621 agcagtggaa gcggccagcc tcaacggccg tgaaattgcg acgccaaatg acgttcggca
    16681 atggctggat ttgaggaagt cgtgagccgg aaaaaagtag tttctgcctt aggaaaatgg
    16741 atttcagaaa aatggtaaaa acaacggaat tgcaacccgc tgtgtcgaaa aataaatctt
    16801 ttaatcgtca gtatccgccc gtcttttttg gaaatttcgc atcggaaaat cattcagaaa
    16861 agggggaaaa gggatgattc ctaaagaaga tcagaacgtt taaaatcata aaacgttgaa
    16921 atggattggt gccggagctc ggaatcgaac cgagatgccc ttgcgagcgg gggattttga
    16981 g
//
LOCUS       DAONQZ010000049        16214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4016_length_16214_cov_16.625224, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000049 DAONQZ010000000
VERSION     DAONQZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_16214_cov_16.625224"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            594..2741
                     /locus_tag="PLR13_08350"
     CDS             594..2741
                     /locus_tag="PLR13_08350"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQC19387.1"
                     /translation="MGVADVKVDFNIAIPMTKAENPVGYNCNIYASAFYHTSPYNGTG
                     SIKRPTIFLATGYHRDLIGLMFAVIAFVPHDYNVVVLDMRGTGSGEGVWDPLSPIEQY
                     DVAYMIDKWIPAQPWSDGTVGMVGGSYMGIIQYLAAGLVEQVNGTPTHLKAIAPISAY
                     NDVWKDIVWHGGNFDLEFMAIWILMTDFISILPPDLIVGGVAEPNFNKTDFMNALSIW
                     AQHFKQLTVPLEWFMDSTKGTRNSWYDTKSPMIYWPDKPAGGWNFPDMPPAVGGGVIP
                     KNLPILTATGWYDIFTRGSFMNYEYGLKNHAPGNKAIIVGPWYHIDAAFMFPGVKGMG
                     IEGKEGIFTWDVLRRWMDWRLKGKDDPFLQEFPVLLYVLGENKWRAEKSWPLPSSRLS
                     NQTYYLSKAKSSLIFGDWFGVANYDDNYKLVPSVKTTDYNNVILGIKKPKVNPVLYHS
                     PTALNGAFSRSAQRWLGFSPLTIVSQLSRYTLDINLDSTLFWEDERSDETGVLTFSTE
                     PLASDMEISGPLKLTFWAKTKFTEPLKQAALDNFIDQIKSTFNIGEDKDMILQIADRK
                     DVQWVVEVNDVFPNGRAKNITSGWLSAAFRPYNAAKPTQVDPAYRAFDPFYFQPEKNP
                     NNIAEETVYQYVVELWPTTNVFKKGHRLRVSISASDFPHLFPVLRPSKNTIVIDEAHQ
                     AKLDFKVANKNGEGTLWKWIDGDVGDYLLTHKN"
     gene            3078..4814
                     /locus_tag="PLR13_08355"
     CDS             3078..4814
                     /locus_tag="PLR13_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQC19388.1"
                     /translation="MNVQDNMSKESEKALFIQKSEEMKKLFVQLLLTSKNVSLYPAGH
                     SISLNSIGNFHETLEAYIRKHGDIRFEFERERVTCQEMNVQTGPPEEGSLPFTLFRDG
                     IRWLEFAEGITLEETRELLTIIHQYSVLTKEPEGDIVTAFWEAHFNHILYQADDFISE
                     QATDSLESMTKFQATPEDQGDGSESADVSETAALHEDAQEPESSEQIGLDPDSFVLTA
                     REQIALQDMIAREEMLSATEHLNMLLDMLLQYKEEKDFSIILEVISEEFDGSIRRHDF
                     EATLIILDGLRRVLDSGRLRSPYAHDLIEKLYGDISSADRCLEPLKDAWSSLNAQQIE
                     IFKNIFQHLQPQAIHTLTEFLLLGQPSQLEQLLQDTIIALINRDTNPLNQLIGKADEK
                     VAEKLVSVLSKVEGNISLKYLMKLARHPSPSARRLAVKTIGQIEGDYIPRIFEFLNDP
                     DESVRRTILKQMSRTRNEAAEDLLLQYLQNHQFDNEEAWYISECLVTLGRCGSERCIP
                     FLRDILLQRKRIAGAKKSVCRQGAAFALAALKMPEASRIIEAAGRSFSPSLRRIARKA
                     EKEVVQKNKKGS"
     gene            4817..6259
                     /locus_tag="PLR13_08360"
     CDS             4817..6259
                     /locus_tag="PLR13_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC19389.1"
                     /translation="MSEQKSISEQWGHDFLRTLYHMTSTVRMYQDNNQIVRTSVESFH
                     KILHELTNDGDISLRLNRGRFHLGGEKIPYRRDAAVVVYNMVDFFSKRSIGSLTFLTS
                     SRDVPPEKIMTLARLLNEAVKHDRHVEWLEEQLTKNDFSWVQIQQLQDEDIIAEGENL
                     EEKKLEKARKNYFQAIEAVKEVANKVSHGTAGVRRIRRLAQNIVDLTKQDAALMIGLT
                     TLKDYDDYTYIHSVNVALLAACLGRHIELSDIVLEHLTICGLFHDLGKVDVPKEILFK
                     QGELTNGEWDTVKKHPVMGVKKILMLNANPSLRSKIILGPFEHHLNLDMTGYPQTLFI
                     NNVSLMGKIIHIADVYEAMTSARVYRPKSFMPDEVLKEMWKEAGTSFDTILLKRFIHM
                     MGTYPIGSVVELNNGSFGVVIDYPEETERSLPLIMLLEDDGKGTWQSGKMIYLADQNS
                     LEVKDRLSILRTISPEKFHIHPAEFFLRLK"
     gene            6340..6966
                     /locus_tag="PLR13_08365"
     CDS             6340..6966
                     /locus_tag="PLR13_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19390.1"
                     /translation="MKKTVFLILLLLSLMACSKSSENAEDMMNEANLLWDGKQYTEPQ
                     KAVEFLSKAIKLQPDDADTYNQRGVAYKNLGNNQLALEDFNAAIRLQPDFVLAYYNRA
                     TVRNNLGQYQEAIADCGEAIRLKPDFAEAYNTRGINHNKLRSYKMAIEDFNKAIKLEP
                     RNANAYNNRGLAYFMLENNTQGCSDVKKACELGFCKALDLIKSRGYCL"
     gene            7073..9370
                     /locus_tag="PLR13_08370"
     CDS             7073..9370
                     /locus_tag="PLR13_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19391.1"
                     /translation="MISFNIFKSFKKPTTIKTLGVKNYQIYASLNRLSPGVDNIHLDV
                     HISSQVHNALKKTAFLLMVKHSGAEFFFKEYKKEFCESEKDALRRICNDVLLDGINKA
                     KKASEVQIDLLAQTALAKLFLEEIQLQYNILVEQIEPLVKMYQLSTKHDQNDVFMIKD
                     KLSEIKRHHHHITRLAGKELFELLADVNTRKLRDMRETHFPPRDILPGFFLANPMLHT
                     NNPSDDFFLIEEYVLMGQRSEDTDNYRSIKMILYDLFGQIDLTNEDGRNATEANERKF
                     QTVHGLVDAENCLYDALIMEPDNIGKMLDFYETEEQYEKAKNNKEQESILNKLKERKE
                     SQKNLLNLFYHQFKVAGLLPQIVANFEMKSVYEKYCPPLSPRQVREFLVNFWKRTSMR
                     KNLKRLKSLYGSQFNFGPLLQTVKHIKDCSTKEKKLYLILFLKQFSRYYRDLYNFYLI
                     QDAMNSINLVTEEKIILLSRENHSLFEFLLPDEKITEEKPIANHVIIKADIRGSITIN
                     HTMRKMGLNPASYFSLNFFDPISAVLAEYGASKVFIEGDAIILSLFENEDASEGNYSV
                     ARACGLAIRILRIVHSYNEKNKENNLPELELGIGICYSQDPPTFLFDGDSKIMISQAI
                     NQADRLSSCDKLLRKIFKSQNDIFNLFVFENEAEEIIEAAGEDATYRYNVNGIELNEE
                     GFHKLTREINLQTYIFPSETDENIKLYAGKVPLTNGNYQRIAIREAPIHKLQPSALDA
                     AGKITKKYYEVCTHPDIYDFTDKQY"
     gene            9517..11133
                     /locus_tag="PLR13_08375"
     CDS             9517..11133
                     /locus_tag="PLR13_08375"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HQC19392.1"
                     /translation="MINKNNSRYPTYRVLLIIAFLAGFVSLAYEIVATKVLYYFFNES
                     TITTSTVISIFLFGIGLGSFIFSRVEKKIPDQRKLILSAQLLIALYAVFIFTDYDLVP
                     SVFNFLYPLFGNDVIMLLFNKLFTSFLYLIFPTVLMGMVFPAIIVMSINAIEQLPQKI
                     GTIYSFDLFGAVLGALFSGFVFIPFFGIKALIFFSVSVNLIIGLLVFFQKNKKFLIML
                     AGFALMLLVFYFIIQPFKSDTAIYSSPDNKEKYIPGENLLFHTGKDTPYFQKVKIKEK
                     IFFSHSPFGELSVFDEVADTEQHRYLYIETRVQCSTVGLKQKEVSEINFANVSIDALD
                     NKNLRVLNIGLGCGFTLHEIVKHPNVKAVDVVEINPVMPEAAKCFSAFTDHVLENPKV
                     KIIFDDGYKYLLGTDKTYDLIIMDIEHPSVIYSSNLYTLEFYKLVKKALHKNGIFTQW
                     SYRPIPEAQVINFNTLKAVFPDVFPKISGVFNDLYYIAGKENIALDEREVTFLNKMLG
                     SNDKRLNTLNNPYFGTEMMLRDLFHVHQDI"
     gene            complement(11364..13802)
                     /locus_tag="PLR13_08380"
     CDS             complement(11364..13802)
                     /locus_tag="PLR13_08380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQC19393.1"
                     /translation="MFKNKKSIWTFLLSTLMFLLMAGAAFAGEADIQLPDLTQVAFMG
                     GSLQGLTILNVGLIICLIGMAFGIMQYVQTKNLPAHKAMLDVSQTIWETCKTYLFQQG
                     KFLIALWILIAICIVYYFGALQGKGVSDIIIILICSILGILGSYTVAWFGIRINTVAN
                     SRAAFSSLSGNPLNIVNICLRSGMSVGLLLVSIELFFMIIILAYIPKELAGPSFIGFA
                     IGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALIAFLALALASNPEMGGKLIVWIFAMRILMILTSLASYFVNDV
                     ISKAAFGGKKEFNFEHPLTNLVWITSIVSIAVTFVASYYLLGDLTEPYTSLWWALAII
                     ISCGTIAGALIPEFTKVFTSTSSRHCEETVNASKQGGPSLNILSGLVAGNFSAFWEGL
                     TILILMFIAYMVSKNPAVMALMPPEFTFAAPVFAFGLVAFGFLGMGPVTIAVDSFGPV
                     SDNAQSIYELSLIESRPDAKDVVKKEYGIAPDFELAKHFLESADGAGNTFKATAKPVL
                     IGTAVVGATTMVFGIIILLEHLFGNVVAKLSLVQPEIILGLIMGGAVIYWFTGASIQA
                     VTTGAYQAVVFIKKNINLDKAEASIEDSKEVVKICTQYAQKGMINIFIVIFFMCLALA
                     FFNPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNAKKIVEVDLKMKNTPLHEASVVGD
                     TVGDPFKDTSSVSLNPVIKFTTLFGLLATEIAVTMTNPNLKTGLAIFFFLIAIVFVYR
                     SFYSMRIASEKLGE"
     gene            complement(13821..14453)
                     /gene="pth"
                     /locus_tag="PLR13_08385"
     CDS             complement(13821..14453)
                     /gene="pth"
                     /locus_tag="PLR13_08385"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQC19394.1"
                     /translation="MLLSVLKKIFFRQVDEREQIMYLMIGLGNPGSRYARTRHNIGFM
                     VLEKIAAQWKISLTQKSFDALWNRGKINNVSVLLAMPQTYMNLSGKSVGRLMAYYKVD
                     IDHVIVIHDDLDLPFGTIRLKKGGGDAGHKGLKSVITALGSADFLRIRMGIGKPADKA
                     GVENYVLQKFNQEEQGVLPESIQLAAEAAADIVISGMQQAMAKYHTKNIK"
     gene            complement(14489..15175)
                     /locus_tag="PLR13_08390"
     CDS             complement(14489..15175)
                     /locus_tag="PLR13_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQC19395.1"
                     /translation="MEVTDLAAQVRKEQKKGPSRRLRDKGFVPAVFYGRSAENILLAV
                     KSDELIKLHKDKKDHAFIKLIIDDEGSKKIEKLSLIKELQLQPLTGKLYHVDFYEVDE
                     KRKITMDVALNFIGKAIGVENGGELQHLKREVKVSCLPLDLPDHIDVDVTDLEIGDSV
                     KIRDLKVAEGVTLLDRPDTSVASVAMIKVAKVEEAAKEEGATEGAPAEGEAAATAGAA
                     AAPAEKEKGK"
     gene            complement(15198..16139)
                     /locus_tag="PLR13_08395"
     CDS             complement(15198..16139)
                     /locus_tag="PLR13_08395"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQC19396.1"
                     /translation="MLERIRIFSGNANKELAEKICNTLGVSLGKANVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCPPVNVTLMELLIMIDAMKRASVDRITAVVPYYGYARQDRKV
                     ASRAPITAKLVADLITAAGANRVLSMDLHAGQIQGFFNIPVDNLFAKPVLLDYMKKTY
                     MDNTVIVSPDAGGVERARSFGKRLGASLAIIDKRREGPNEAEAMNIIGDVKGKRVIIL
                     DDMIDTAGTVVQAASAMKKAGALEISVCCTHPVLSGPALDRIENSDIKEAIVTDTIPL
                     SDRAKNCGKIKVLSVSGILGEAVRRIYYNDSVSSLFI"
BASE COUNT         4562 a         3777 c         3684 g         4191 t
ORIGIN      
        1 aatcctctcc ccgctaccaa gtaattacaa ggagttagct gatgcagtta gctctttttt
       61 tgcattttgg aggggctttg agctaccttc tgcaataata catttccccc atgaaatatc
      121 ttctttgtgc gccttctttc aatgttttcg ttttgcacgc tcatttcttc cgacacaact
      181 ttcaggaata gtccccaaat atcacatctc gtttccataa gtatattgac atcgcgggat
      241 aaatcgttta gtgttccaat cactcatcaa tatcttacca attgaaaaaa agcacggggg
      301 gaggagtttg taaaatccac tgccaaacaa cattgccatg aaagggaggg atcatgaaac
      361 aggttgattt gctgcgtcta gccaaatgtt gtggtaccat tgtaataggt tgttttttaa
      421 agggtttgat ttttgcagaa acgaaaaaca gatggttcca atcgcggcat cgtttcaaga
      481 tctggtccat attcctcgct gtgacagtgg ggattcttat aacctccgct catgttcaag
      541 ctaatacttc ggatgctgct gctcaaacgc agatacagga agcgctggca aaaatggggg
      601 ttgcggatgt gaaggttgat ttcaacatcg caattcccat gaccaaagct gaaaatccgg
      661 tcggatacaa ctgcaatatc tacgcctcgg ctttttatca tacgtctcct tacaatggca
      721 cgggaagcat caaacggccg acgatttttc tcgcaaccgg ttaccaccgg gacctcatcg
      781 gtctgatgtt cgccgtgatc gcctttgtgc cccatgacta caatgtcgtt gtgttggata
      841 tgcgcggaac cggttccggt gaaggcgtct gggatccact cagccctatt gaacaatatg
      901 atgtggccta tatgattgac aagtggattc ccgcgcagcc gtggtcggac ggtacggtcg
      961 gcatggtcgg aggctcctat atgggcatca tccaatatct tgccgccggt cttgtcgaac
     1021 aggtcaacgg cactccaacg catcttaaag ctattgcgcc gatttcggcc tataacgacg
     1081 tctggaaaga cattgtctgg cacggcggga attttgatct ggaattcatg gccatctgga
     1141 tccttatgac ggattttatc agtattcttc cgccggacct gattgtcggc ggtgtggcgg
     1201 agccgaactt caacaaaaca gatttcatga acgcattgag catctgggca cagcatttca
     1261 aacaactgac cgtgccgctg gaatggttta tggattcgac caaaggaacg agaaacagct
     1321 ggtatgacac aaaatcgccg atgatttact ggccggacaa accggccggc ggctggaatt
     1381 ttccggatat gcccccggcc gtgggcggcg gcgtcatccc gaaaaatctt cccatcctaa
     1441 ccgcaaccgg ctggtacgat atcttcacca gaggttcttt catgaactat gaatatggat
     1501 tgaaaaatca tgctcccggc aataaggcca tcattgtggg gccctggtat catattgatg
     1561 cagcgtttat gtttcccggc gtcaagggga tgggcatcga agggaaggaa ggtattttca
     1621 cgtgggatgt cctccgccga tggatggact ggagactcaa gggaaaagac gatccgttcc
     1681 tgcaggagtt cccggtgctg ctctacgtac tgggagaaaa caaatggcgt gctgaaaaat
     1741 catggccttt gccttcatcc agactcagca accaaacgta ttatctttcc aaagctaaat
     1801 cctctctgat tttcggcgat tggtttggag ttgccaatta tgacgacaat tataagctgg
     1861 tccccagcgt gaagaccaca gattataata acgtgatttt ggggattaaa aagccgaaag
     1921 tgaaccctgt tctatatcat agcccgacag cgctcaacgg ggcgttctcc cgctcggctc
     1981 aacgctggct gggattctcg cctctgacga ttgtgagcca gttgtccagg tacacactgg
     2041 atatcaatct tgacagcaca ctattctggg aagatgagcg cagtgatgaa accggtgttc
     2101 tgaccttttc gactgaaccg ctggccagtg atatggaaat ttccggtccg ttgaagctga
     2161 ccttctgggc aaaaacaaag tttaccgagc cgctgaaaca ggcggctctc gataacttca
     2221 ttgaccagat caaatccacg ttcaacattg gagaggacaa ggatatgatc ttacagattg
     2281 ccgatagaaa agatgtccag tgggtggtcg aggtcaatga tgtgttcccc aatggtcggg
     2341 caaaaaatat tacatccgga tggctcagcg cggcgttccg cccttataat gcggctaaac
     2401 ctacgcaggt ggatccggca tacagagcat ttgatccttt ctattttcaa ccggaaaaga
     2461 atccaaacaa tattgctgaa gaaacggtct atcagtatgt ggtcgaattg tggccgacaa
     2521 ccaatgtttt caagaagggg caccgcctcc gcgtgagtat ttccgcttcc gatttccccc
     2581 acttgtttcc tgtgcttcgg ccgtcgaaaa acaccatcgt tattgatgag gctcatcagg
     2641 ccaaacttga tttcaaagtt gccaacaaga atggggaagg aacgttgtgg aaatggattg
     2701 acggagacgt cggcgactat cttttgaccc ataagaatta acagtatcca ataatcaaaa
     2761 ggaaaagggt ggcgcgaaag caccaccctt ttttcttgct catcaagccg tattaaagat
     2821 attcaagata agactttagg ctatgaagat tgtcttgaca gaaaaaacta atttttctat
     2881 actttttaca tacaaagcaa ttgccgttac actcaaaaat cgcagttttt ttgtgattcc
     2941 acagattaaa gtgcctgaaa gaacgcttca ggaccgagat caagtaccgg ctttgctttt
     3001 gtccgttatt ttgaaaactt tatcaggatg gtacaagcat catcaataaa aaatgattca
     3061 tcgccagcgg taccgaaatg aatgtccaag ataacatgag caaagaatcc gaaaaagccc
     3121 ttttcattca aaaatcggaa gaaatgaaaa aattattcgt tcagcttctg ctgacgagca
     3181 agaacgtttc tttatatccg gcaggtcaca gtatcagtct gaattctatc ggaaattttc
     3241 atgaaacgct ggaagcctac attcgcaagc acggcgatat ccgatttgaa tttgaaaggg
     3301 agcgcgtcac ctgccaggag atgaacgttc aaacaggacc gcctgaagaa ggatccctgc
     3361 cttttacgct tttccgcgac gggatacgat ggctggaatt cgccgaaggg ataacgctgg
     3421 aggaaacccg cgaattatta acgattattc accaatattc cgtactgacc aaggaaccgg
     3481 aaggggatat cgtcaccgct ttctgggaag cacatttcaa tcatatcctg tatcaggctg
     3541 acgattttat ttcagaacaa gcgacggatt ctttggaaag catgacgaaa tttcaggcaa
     3601 ccccggaaga tcaaggagac ggttctgaat ccgccgacgt atcagaaacc gcggctttac
     3661 acgaagatgc acaagagccc gaatcttccg agcaaatcgg gctggatccc gattcttttg
     3721 tgctgacagc cagagagcaa attgccctgc aggacatgat tgcccgcgaa gaaatgttgt
     3781 ccgcgacaga acatttgaat atgttgctgg acatgcttct gcagtataag gaggagaaag
     3841 atttcagtat catcctcgaa gtcatatcgg aagaatttga cggttcgatt cgccgtcatg
     3901 attttgaagc aaccctgatt attcttgacg gactccgcag agttttggat agcggccgtt
     3961 tgagatcccc ttacgcgcac gacttaatcg aaaaacttta cggagatatt tcatcggctg
     4021 accgctgcct ggaaccgttg aaggatgcct ggagttcctt gaatgcacag cagatagaaa
     4081 tctttaaaaa catttttcag catcttcaac cgcaggcaat ccataccctg acagaatttc
     4141 tgcttttagg gcagccgtca cagctggagc aacttctgca ggatactatt atcgccctca
     4201 tcaatcgcga taccaatccg ctgaaccagt tgatcggaaa ggccgatgaa aaagtcgccg
     4261 aaaagcttgt ttccgtgctg tcgaaagtgg aggggaatat ttccctgaag tatctgatga
     4321 aacttgcgcg acatccttcg ccgtccgccc ggcggctggc cgtcaaaacc atcgggcaaa
     4381 tcgagggtga ttacatcccc aggatcttcg aattcctcaa cgatccggat gaatcggtcc
     4441 gccggacaat tttgaagcaa atgagcagaa caaggaacga ggcggctgaa gatctcttac
     4501 tgcaatatct tcagaaccat caattcgata acgaagaggc ctggtatatt tcggagtgcc
     4561 tggttactct gggcaggtgc ggatcggagc gttgcattcc ctttttacga gatatcctgt
     4621 tacaacgaaa gcggatcgcc ggcgcaaaga aatcggtatg ccgtcagggg gcggcgtttg
     4681 cgctggcggc tctaaaaatg ccggaagcca gtcggatcat tgaggcagcg ggacgcagtt
     4741 tcagccccag cctgcgcaga atcgcccgga aagcagaaaa agaagttgtt cagaaaaaca
     4801 aaaaaggatc ataatcatgt cggaacaaaa atcgatttcg gaacaatggg gacatgattt
     4861 tctgagaacc ctgtatcata tgaccagcac agttcgcatg tatcaggaca acaatcagat
     4921 tgtccgtaca agcgtcgagt cttttcataa gatcctccat gaattgacaa acgacggaga
     4981 tatcagcctc cgtcttaacc gaggccggtt tcatctgggc ggagaaaaaa ttccctatcg
     5041 cagagatgca gcggttgttg tttacaatat ggtggatttc ttctcaaagc ggagcatcgg
     5101 aagcctcacc tttttaacat cctcacgcga tgtgccgccc gaaaaaatca tgaccctggc
     5161 acgtctgctc aatgaggccg tcaagcacga tcgtcatgtg gaatggcttg aagaacaatt
     5221 gacgaaaaat gatttttcat gggtgcagat tcagcaactt caggatgaag acatcatcgc
     5281 cgaaggggaa aacctggaag aaaagaaact tgaaaaagcg cgaaaaaatt attttcaggc
     5341 gatcgaggcg gtcaaagaag tggcgaataa agtatcccat ggaacggcgg gagtccgcag
     5401 aatcaggcgt ttggcacaaa acatagtgga tctgacaaag caggatgccg cgttgatgat
     5461 cggcctgaca acgctcaagg attatgatga ttatacctac attcattccg taaacgtcgc
     5521 gcttctggcc gcctgtctgg gaagacatat agaattatcg gatatcgtgc tggaacattt
     5581 gacgatttgc gggttgtttc atgatttggg gaaagtggac gttcccaagg aaattctctt
     5641 taagcaaggt gagttgacca acggggaatg ggacacggtc aagaaacacc ctgtcatggg
     5701 ggtcaaaaaa attctcatgt tgaatgccaa tccgtcctta agatcaaaaa tcattctcgg
     5761 gcctttcgag catcacctga atctggatat gaccggttat cctcaaacgc tttttatcaa
     5821 taatgtaagc cttatgggca agattattca tatcgccgat gtttatgaag ccatgaccag
     5881 tgcgagggtt taccggccaa aatctttcat gccggatgaa gtccttaaag agatgtggaa
     5941 agaagcgggg acaagcttcg atacgattct gttgaagcgc tttattcaca tgatgggaac
     6001 ataccctata ggatccgttg tagagctcaa caacggatcc ttcggcgtgg tgattgatta
     6061 ccctgaagaa actgaacgca gtcttccgct tattatgctt cttgaagatg acgggaaagg
     6121 aacctggcaa agcggaaaga tgatttatct ggcggatcaa aattctctgg aggttaaaga
     6181 ccgattgagt atcctccgaa caatttcgcc ggaaaaattt cacattcatc cggcggagtt
     6241 ttttctgcgt ctaaagtaac agaaatattt ttattatatt tttccgataa ttatgctagt
     6301 gaagttgcat actaaacact atttatggag gcagatatta tgaaaaaaac ggttttcctt
     6361 atccttcttc ttttgagcct gatggcctgc tcaaagagtt ctgaaaatgc tgaagatatg
     6421 atgaatgaag caaacctctt gtgggacggc aaacaatata cggaaccgca aaaggcagtc
     6481 gaattcctaa gcaaagccat taagctgcag cctgatgacg ccgataccta caaccagcgg
     6541 ggagtcgctt ataagaatct ggggaacaat cagcttgccc tggaagattt taatgcggcc
     6601 atccgcttgc agccggattt tgttctggct tattataacc gcgcaaccgt ccgaaacaat
     6661 ctgggccagt atcaagaagc aatcgccgac tgcggcgaag ccatccgtct caaaccggat
     6721 tttgcagaag catacaatac acggggcatc aatcataaca agctcagaag ctacaaaatg
     6781 gcgattgagg atttcaacaa ggccattaaa ctggaacccc gtaatgccaa tgcatacaac
     6841 aatcgcggcc tggcttattt tatgctggaa aataacacgc agggatgcag cgacgtgaag
     6901 aaggcctgtg aacttggatt ttgtaaggcg ttggatttga ttaaaagtcg cggatactgt
     6961 ctctaggccg gcattgatct gatcgtgaaa attattcatg catcgtatcc gtttcatgaa
     7021 ttattggcgg accgaatagt gattcgatta tccaataagc ataaagggtg tcatgataag
     7081 ttttaatatt ttcaaatcat tcaaaaaacc gacgacgatt aagacgctgg gtgttaaaaa
     7141 ctaccagatc tatgcgtctc tgaatagact ttctcccgga gtggacaaca ttcatctgga
     7201 tgtccatatc agctcccagg tgcataacgc ccttaaaaag acagcctttc tcttgatggt
     7261 aaaacacagc ggggctgaat tctttttcaa agaatataaa aaagagtttt gtgaatcgga
     7321 aaaagacgcc ctgagacgaa tctgcaacga tgttttgctg gatggaatca ataaagcgaa
     7381 aaaagcgtcg gaagtgcaga tcgatctgct ggcgcagacg gctctggcaa aactttttct
     7441 ggaagaaatt cagttacagt acaacattct ggtggaacaa atcgaacctc tggtcaagat
     7501 gtaccagttg tcgaccaaac atgaccagaa tgatgtgttc atgatcaaag ataagctgtc
     7561 ggaaataaaa cggcatcatc atcacatcac ccgcctcgcg ggaaaagagc tctttgagct
     7621 cttagccgat gtcaacacgc ggaagctcag agatatgcgg gaaacgcatt ttcctccgcg
     7681 ggacattttg cccggctttt ttctggcaaa tccaatgctg cacacgaata atccgagcga
     7741 tgattttttt ctgatcgaag aatatgtact catggggcag cggtccgaag atactgacaa
     7801 ttacaggagc atcaaaatga tcctgtacga cctgttcgga caaatcgatt taacaaacga
     7861 agacggccga aatgcgacag aagccaatga gcgaaaattt cagacggttc acggcctggt
     7921 cgatgcggag aattgtttat atgatgcgtt gatcatggaa ccggataata tcggcaagat
     7981 gctcgacttc tatgagacag aggaacaata tgaaaaggca aaaaacaaca aagaacagga
     8041 aagcattctc aataagttaa aagaacgaaa agagagccag aaaaacctgt tgaatctttt
     8101 ttatcatcaa ttcaaagttg ccggcctgct gcctcaaatt gtcgccaatt tcgagatgaa
     8161 atccgtctac gaaaaatatt gtcctccgtt gtctccccgg caggtaagag aatttctggt
     8221 taatttctgg aaaagaacat ccatgagaaa gaacctgaaa cgattgaaat ccctttacgg
     8281 tagtcagttc aatttcggcc ctctcctaca aacagtcaaa cacatcaaag actgctccac
     8341 aaaagaaaaa aaattgtatt tgattctgtt tcttaaacaa ttttcccgat actaccgcga
     8401 cctgtataat ttttatctta tccaggatgc gatgaattcc attaacctcg ttacggagga
     8461 gaaaattatt ctgttatcac gggaaaacca ctcgcttttt gagtttctgc tgcccgacga
     8521 aaaaattacg gaagaaaaac ccatcgccaa ccatgtcatt attaaagcgg acatccgcgg
     8581 atctattacg atcaatcata ccatgagaaa aatggggctg aatccggcct cctatttcag
     8641 cctgaatttt tttgatccca tatcggctgt tcttgccgaa tacggggcct ccaaggtctt
     8701 tattgaaggg gacgccatta tcctgtccct tttcgaaaat gaggacgcat cggaaggaaa
     8761 ttacagtgtg gccagagcgt gcggtctggc catcagaatc ctgcgcattg ttcatagtta
     8821 taatgaaaaa aacaaggaga acaatcttcc ggaacttgaa ctgggcatcg gcatttgtta
     8881 ctctcaggat ccgccgacgt tcctgtttga tggcgattcc aaaatcatga tctcacaggc
     8941 catcaatcag gcggaccgcc tgtccagctg tgacaaatta ctgcgaaaaa tatttaaaag
     9001 ccagaatgat attttcaatt tgtttgtgtt tgaaaatgaa gcggaagaaa tcattgaagc
     9061 ggcgggagaa gacgccacgt atcgctacaa cgtcaacggg attgaactga atgaagaggg
     9121 cttccataaa ctcacccggg aaattaacct tcaaacctat atctttcctt ccgaaaccga
     9181 tgaaaatatc aaattgtatg cggggaaggt accgttgacc aacggcaatt atcagcggat
     9241 tgccatccgg gaagcgccca ttcacaaatt acagcctagc gctctggatg cagccggaaa
     9301 gataacaaaa aaatactatg aggtttgcac acatccggat atttacgatt ttaccgacaa
     9361 acaatattaa ttgaatttcc ttgatccgga tgtttcaata gcgtatcaga taaaactggt
     9421 ttatcgttcc cgggaattta agatatcctt ttctgtccga ttccatggcc tccggaagat
     9481 actgagatta ttgatcggag ttttcgtaaa aaagcaatga taaataaaaa caacagccgt
     9541 tacccgacgt atcgcgtcct gctcattatc gcgtttctgg ccggttttgt cagtctggct
     9601 tatgaaatag ttgcaacaaa agtactgtat tattttttca acgaaagcac cattacgacc
     9661 tccactgtga tctccatctt tcttttcggt atcgggctgg gaagttttat tttcagccgg
     9721 gttgagaaaa aaatccccga tcaacgcaaa ttaattcttt cggctcagct gctgatcgct
     9781 ctttacgccg tttttatttt cacggattat gatctggttc catcggtttt caatttcctt
     9841 tacccgttgt tcggaaacga tgtcatcatg ctgctgttca acaaactgtt cacaagtttt
     9901 ctctatctta tttttcccac ggttctgatg ggcatggtct ttccggccat tatcgtcatg
     9961 tctatcaatg cgatagagca attgccgcag aaaataggca cgatatacag tttcgatctt
    10021 tttggagcgg tactgggagc attgttcagc ggttttgttt tcatcccctt cttcggcatc
    10081 aaagccctga ttttcttttc cgtctcggtc aacctgatca tcggtctgct tgttttcttt
    10141 cagaaaaaca aaaagttcct cattatgctc gccggtttcg ctttgatgtt gcttgttttc
    10201 tatttcatta ttcagccgtt taaaagcgac acggccattt attccagtcc cgacaacaaa
    10261 gaaaaataca tccccggtga gaatcttctt ttccataccg ggaaagacac cccctatttt
    10321 cagaaagtaa aaatcaaaga aaaaattttt ttttctcatt cgccgttcgg tgagctaagc
    10381 gtgtttgatg aagttgcgga cacggaacag catagatatt tatacatcga aacacgcgtt
    10441 cagtgctcga cggtgggatt aaaacaaaaa gaagtgagcg aaattaattt tgccaatgtc
    10501 tccatcgatg ccctggacaa caaaaatctt cgggttttaa atatcggtct gggatgcggt
    10561 tttacgcttc acgaaatcgt caagcatccg aacgtgaaag ctgttgatgt ggtagaaatt
    10621 aacccggtaa tgcccgaagc cgcaaaatgc ttcagcgcat tcacggatca tgttctggaa
    10681 aaccccaaag taaaaatcat ttttgacgac ggttacaagt atcttctggg caccgacaaa
    10741 acatatgacc tgattatcat ggatatcgag catccgtccg ttatttattc aagcaatctc
    10801 tacacgctgg aattttacaa actggtcaaa aaagcgctcc ataagaacgg gatctttacg
    10861 caatggtcct acaggcccat acccgaagcg caggtcatta attttaatac tcttaaggct
    10921 gtattcccgg atgtttttcc gaaaatatcc ggcgtcttta acgatcttta ttatattgcc
    10981 ggaaaggaaa atattgctct ggacgaacgg gaagtgacgt ttctaaacaa aatgctcggc
    11041 tccaatgaca aacgactcaa caccctgaac aatccatact ttgggacaga aatgatgttg
    11101 agggatctgt ttcacgttca tcaggatatc tgaaaaacaa tcagatacgc aggagggaaa
    11161 agctgccatc gcaatccttt tggttatgca gctttaatca acgcctggcc ggatggagac
    11221 cggataccaa agcacatgga tatgatcgga ttgcaaaagc acttcaaaaa acagtcaata
    11281 atattatgaa gcagccaaaa tcgatgtaaa aaaggcgccc ctttttcaaa gggcgccttt
    11341 tggtttaaag agttgagtac ttcttattca ccaagttttt cactggcaat tctcatgcta
    11401 tagaatgaac gatacacgaa aacaatcgcg atcaggaaga agaaaatagc cagacccgtc
    11461 ttcagattcg ggttggtcat ggtcaccgcg atttccgttg ccagcaatcc gaacagggtc
    11521 gtgaacttga tgaccgggtt caaagaaacc gaagaggtat ctttgaaggg atcgccaacg
    11581 gtgtcgccga caacgctggc ttcatgcagc ggggtgtttt tcatcttgag gtcgacttca
    11641 acaatcttct tggcattgtc ccagcaaccg ccggcattgg ccatgaagat cgcctggaag
    11701 agaccgaaga aagcgatacc aatcagataa ccgatgaaga agtaggggtt aaagaaggcc
    11761 agcgccagac acatgaagaa gatcacgatg aagatgttga tcataccctt ctgagcatac
    11821 tgggtgcaga tcttgacaac ttctttactg tcttcaatcg aagcttccgc tttatcgaga
    11881 ttgatgtttt tcttaatgaa gacaacggcc tgataggcac cggtggtaac cgcctgaatg
    11941 gaggcgccgg tgaaccagta aataaccgcg ccgcccatga tgagaccgag gataatttcc
    12001 ggctgaacca aagacaattt ggcaacaaca tttccgaaga gatgttcgag cagaatgata
    12061 ataccgaaca ccatggtggt tgcgccgaca acggcggttc cgatcaacac gggtttggct
    12121 gtggctttga aggtattgcc ggcgccgtca gcagattcca ggaagtgctt ggccagttca
    12181 aaatcgggcg caatgccgta ttccttcttc acaacgtctt ttgcgtcggg acgtgactca
    12241 atcaatgaaa gttcgtaaat ggactgagcg ttgtccgaaa ccggtccgaa gctgtccaca
    12301 gcaatggtca cagggcccat gcccaggaaa ccgaaggcca ccagaccgaa agcaaacacg
    12361 ggagcagcaa aggtgaattc cggaggcatc aatgccataa cggccggatt cttggatacc
    12421 atgtaagcaa tgaacattag aatcaaaatg gtcaggcctt cccagaaagc agagaagtta
    12481 ccggcaacca gacccgacag aatgttcaaa gaaggaccgc cctgcttgga agcattaacg
    12541 gtttcttcgc aatgacggga ggacgtactg gtgaatactt tggtgaattc cggaatcaat
    12601 gcgccggcga tggttccgca actgataatg atggccagcg cccaccagag acttgtgtaa
    12661 ggttcagtca gatcgcccag aaggtaataa ctggcgacaa acgtaaccgc tatggaaaca
    12721 atagaggtaa tccagaccag gttggtcagc ggatgttcaa agttaaattc tttcttgcca
    12781 ccgaaagccg ccttgctgat aacgtcattg acaaaataag aagcaagaga ggtcaagatc
    12841 atgagaattc tcatggcaaa aatccagacg atcagtttac cgcccatttc tggattgctg
    12901 gccaaagcca gagccagaaa agcaatcaac gcaacacctg tcacaccgta ggtttcaaaa
    12961 ccgtctgccg tggggccgac gctgtcgccc gcgttgtcac cggtacagtc ggcaataaca
    13021 ccggggtttt tgggatcgtc ttcgggaaga tggaaaacga tcttcatcag gtcggaaccg
    13081 atgtccgcaa tcttcgtgaa gataccgccg cagatacgca gagcggaagc gcccagcgat
    13141 tcgccgatgg caaaaccgat aaagctgggg ccggccagtt ctttgggaat gtaggccaga
    13201 atgataatca taaagaagag ctcaatgctg accagaagaa ggccaacgct cataccggaa
    13261 cgcagacaaa tgttaacgat gttgagaggg ttgccgctca atgaagaaaa agcggcgcgg
    13321 gagttggcga cggtattgat acggatacca aaccaggcca ccgtgtagga ccccagaata
    13381 cccaagatcg agcatatcag aataatgatg atgtccgaaa ctccctttcc ctgcagagcg
    13441 ccaaaatagt aaacgataca gatcgcaatc agaatccaca acgcgatgag aaatttgccc
    13501 tgctggaaca aataggtttt gcaggtctcc caaatggtct gggaaacatc cagcatcgcc
    13561 ttgtgcgcgg gcaggttttt ggtttgcaca tactgcatga tgccgaaagc cattcctatt
    13621 aaacagatga tcaatcctac attgaggatt gtcaatccct gaagagaccc gcccataaat
    13681 gcaacttggg tcagatccgg caattggatg tctgcttcac cggcaaacgc tgcgcccgcc
    13741 atgagcaaaa acatcaatgt gctcagtaaa aacgtccaga tacttttttt gtttttaaac
    13801 attccattcc tcctttaaaa ttacttaata ttttttgtat ggtatttagc catagcttgt
    13861 tgcatccctg atatgacaat gtccgcagca gcttccgctg ccagttgaat gctttcagga
    13921 agcacaccct gttcttcctg attaaacttc tgcaaaacgt aattttcaac gccggctttg
    13981 tccgccggtt tgccgatacc cattctgatt cgcaggaaat ccgcggatcc cagagcggtg
    14041 ataaccgatt tcagcccttt atgtccggca tctcctccgc cttttttgag acgaatcgtt
    14101 ccgaacggca gatcaagatc gtcatgaatg acgatgacat ggtcaatgtc caccttgtaa
    14161 taggccatta atcgaccgac cgatttcccg ctaagattca tgtaggtctg aggcatcgcc
    14221 agcagtacgc taacgttgtt gatcttacct cgattccaaa gagcatcaaa acttttttgc
    14281 gttaaactaa ttttccattg ggcggcaatt ttttccaaca ccatgaaacc gatgttgtgt
    14341 cttgtccgtg catagcggct tccgggattg cccagtccga tcatcagata catgatttgc
    14401 tccctctcgt caacttgccg gaaaaatatt ttctttaaaa ccgagagaag cacccgctat
    14461 cacccaatta ttgcactttt ctataaagtt acttcccctt ttctttttct gccggagcag
    14521 ccgcagcgcc agctgttgcc gctgcttcac cttccgccgg agcgccttca gtagcgcctt
    14581 cctctttggc agcctcttct accttggcaa ccttgatcat ggcaacagag gcaaccgacg
    14641 tatcgggtct gtccagaagc gttacccctt cagccacttt cagatcccgg atcttaacgg
    14701 aatcgccgat ttccagatcg gtcacatcaa catcgatatg atcgggaaga tccagcggga
    14761 ggcaggaaac tttgacctct cttttcaagt gctgtaattc accgccgttt tccacgccga
    14821 tggctttgcc gatgaaattc aacgccacat ccatggtgat cttgcgtttt tcatccactt
    14881 catagaaatc gacatggtag agttttccgg tcagcggctg aagctgcagt tctttaatca
    14941 gagacagttt ttcaattttt ttgcttcctt catcgtcaat gatcagttta atgaatgcgt
    15001 gatctttttt gtctttgtgc agtttaatca gttcatcgct ttttacagcc aacagaatat
    15061 tttcagcaga gcggccgtaa aaaaccgccg ggacaaatcc cttatcccgt aaacggcggg
    15121 atggtccttt cttttgttct ttacggactt gagccgctaa atctgttacc tccatttgtt
    15181 cctcctcata taaattccta tataaatagt gagcttacgg aatcattgta ataaatacgt
    15241 cgcacggctt cgcctaaaat tccggatacc gataacactt ttattttccc gcaatttttc
    15301 gcccggtcgc tcagcggtat ggtatcggtg acaatcgctt ctttaatatc ggaattttca
    15361 atgcgatcca gagccggtcc cgacaacaca gggtgcgtac aacacaccga aatttccagc
    15421 gcgcctgctt tcttcatggc gcttgcagcc tgcacaaccg ttcccgctgt atcaatcatg
    15481 tcatccagaa tgatcaccct ttttcccttg acatcaccga taatattcat ggcttccgcc
    15541 tcattggggc cttctcttcg tttatcgata atcgccagag atgcccccag tcgttttcca
    15601 aaagatctgg ccctttccac acctccggca tccggcgaaa cgatcaccgt attatccatg
    15661 taggtttttt tcatatagtc cagcagaacc ggtttggcaa aaagattatc caccggaata
    15721 ttgaaaaatc cttgaatctg gcccgcatgc aaatccatcg acaggacgcg gtttgccccg
    15781 gcggccgtaa tcaaatccgc caccaacttt gccgtaatgg gagcgcggga ggctactttt
    15841 cgatcctgcc tggcgtaacc ataatacggg acgactgcag tgattctatc aacagacgcc
    15901 cttttcattg catcgatcat gatcagcaat tccatcaatg ttacattgac cggcgggcac
    15961 gtagactgaa tgataaagac atccattcct ctgacatttt catcgatttc tacgcgggtt
    16021 tccccgtcgc tgaaggtggt gacattggcc tttcccagag acacgcctaa tgtattgcaa
    16081 attttctcag caagttcttt gttggcattt cctgaaaaaa ttctaattct ttcaagcatt
    16141 taaaaaatac tccgcatttt tttctggctg gggcgggagg attcgaacct ccgaatgcag
    16201 gaaccaaaat cctg
//
LOCUS       DAONQZ010000050        15795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4146_length_15795_cov_15.891741, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000050 DAONQZ010000000
VERSION     DAONQZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15795
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4146_length_15795_cov_15.891741"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..744)
                     /locus_tag="PLR13_08400"
     CDS             complement(259..744)
                     /locus_tag="PLR13_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HQC19397.1"
                     /translation="MKLTETQMIEIAQHAFTRMDGAWFLALAKKFGVQTAWEMDVEAW
                     KHFAYVFGKNLRKNYLPNPVWPESIFDALDIFSRVLKIEGRKVCIEDGKIIIRITDCE
                     TQKAIAKAGVADCGIVTVQTYQGLIGGLFDNKIFVDVAHTKNLNRGDDCCEVVVTRKS
                     P"
     gene            1442..1723
                     /locus_tag="PLR13_08405"
     CDS             1442..1723
                     /locus_tag="PLR13_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQC19398.1"
                     /translation="MIKTFADKHTRDLFITGKSKKFQPDILKRAIRRLEYIDLATCIE
                     DLKVPPSNHLHMLKDNREGQYAISVNDQRRICFRFINGDAYDVEITDYH"
     gene            1737..2051
                     /locus_tag="PLR13_08410"
     CDS             1737..2051
                     /locus_tag="PLR13_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HQC19399.1"
                     /translation="MSIKYDGKRKVKPTHPGEILKEDFMPDYNLNVATLAKALGVSRQ
                     TVNELLRESRALTPIMALRLGRLFGNTAEFWLNAQRAHDLWEAQQTHQAEIKKIHPLK
                     AA"
     gene            2209..3066
                     /locus_tag="PLR13_08415"
     CDS             2209..3066
                     /locus_tag="PLR13_08415"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQC19400.1"
                     /translation="MNKSKDKLSALIIYLSPHGTTKKAIDTLAVSLNENSVSSDIYNL
                     SEFDNNDIMAKIADYQLIVLGSPTYFHHAPTVFTDFINNMPDASNDQSVVLLSSFGGV
                     SSGVVLYDLAKILHRKKYNLIGGIRVLTEHCLTFQEEKPFYSGHPDKKALNAIKTFGK
                     EISVRLKNKICTRYSPSDFNDKPFILNFMDDHIVKLRNFVWAMPGITVNQSLCNRCAV
                     CVKNCPTHNIKLGKATMHGGNCIYCYCCVRNCPVHATIAFLKPASITVKLMAEIFRKF
                     EDQETQQIV"
     gene            complement(3159..3299)
                     /locus_tag="PLR13_08420"
     CDS             complement(3159..3299)
                     /locus_tag="PLR13_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19401.1"
                     /translation="MDELFIAEAIIRQVLRRMPDAPDRLLIAPKYSGSIEGRRLNQRK
                     NR"
     gene            complement(3307..4344)
                     /locus_tag="PLR13_08425"
     CDS             complement(3307..4344)
                     /locus_tag="PLR13_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQC19402.1"
                     /translation="MRISLKSKPVIAPYRSTHNLRVIIQWAEEHGIDTASILSSEGLE
                     KADFDNPDRLITFEQQFEISQKLFDLISCPALGLRIGERINIGQLGKWIMAINSCATT
                     LDAFKMAFELADLLSFPFQFILDVQDDIAKFSFIDLIDLGRSSRFRYEMHMVGGYNIC
                     CEIMRERVQLIEIRFAYSKPEYAEEYKPIFNCPILFDAEETQIRFDSSYLTRPLPCAN
                     TLTKKIYEKDCRQTLEQLIMLTTTTELAKSALQFKDKELLTRSQLARCLNMSDQTLKR
                     RLAAEGVSYKSLVKDIRQKKACDLLQSTELTMEEIANKVGYRDVANFYRAFKSWTGLT
                     PRSCRGKNEKR"
     gene            4567..6084
                     /locus_tag="PLR13_08430"
     CDS             4567..6084
                     /locus_tag="PLR13_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19403.1"
                     /translation="MRKGLRLFPMMTMLILFVLTVFSVEIFAWPVIPPPNFTNEPPRN
                     PFIADSPWPMSHRNPYGQASSPYPGPKSINLTTRSSFHVGMPGLITLAISGKYPDGKR
                     VIWGSSSTDVFKADESLNYIKTMKKENLTISSIFTPGEMLSGAYTLVDKDNTFYMPRF
                     TKIFAFGDAIAGNRYSDIIIQGVYEIPANLLVDPAEKIVGLSMTWDGMLTLATSHGLI
                     GVVSRFFDEAYYYRFPADEQISNSIACDEHGGIYVVTDKNMYRVQWTGSELTIDPDKG
                     GWSAGYETGEASGVRLGAGSGSTPTLMGTGNQDKFVVITDGQNLMHLVLFWRDEIPAD
                     WQQIPGTKSRRIAAQVPVTFGNPGATETLSEQSVCVRGYGAVVVNNQLKASLDNQVIS
                     VLMSGFAPIAPYGVEKFVWDPWTRTLKTAWVNKSVSIPNGIPTMSQTTNLFYGVGKRF
                     GFWTFEALDWTTGKSVFSYPYGITPANNSAYAATEIGLNGSLLTGTLTGMVLMKP"
     gene            complement(6257..8953)
                     /gene="fdhF"
                     /locus_tag="PLR13_08435"
     CDS             complement(6257..8953)
                     /gene="fdhF"
                     /locus_tag="PLR13_08435"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7883..7885),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQC19404.1"
                     /translation="MEKKIPIRIDGKDVEATAGQTILECARDNGIFIPTLCHYTKTTN
                     VGACRVCVVEVEGARSLAASCCMPVTPNMVVRTDTEAVRKAQKMIIELLWSSGDHNCL
                     TCEQNGNCELQNLVYWLQIDKPRFEIQVPGYAKDDSNTMIARDMNKCILCGRCVRACN
                     EIQVNEVLDFANRSYKAKVGPAFDQDYIDSTCVFCGECVQACPTGALTFKQAKFAGRP
                     WELAKVKTTCTYCGVGCQMDLYTKDNKIVKVMGNREYGQPNDGSLCVKGRFGMDFLSH
                     PDRLKTPLIRYKKNENFKEATWDEAFTFIADKLSRIKKEHGADSIAGLASARCTNEEN
                     YLFQKFLRAGIGTNNVDHCARLUHSVTVAGLAATFGSGAMTNSIDEIEFTDLILATGT
                     NTTENHPVIGAKVKRATKRGAKLIVIDPREIDLVKYADIWLRQNPGTDVAVINGMMNV
                     IISEGLYAKEYVKDRTEGFDALKKMVEKYTPEKVEKISGVPAEDLKKAARMYAKADRA
                     SILYAMGITQHITGTDNVKSCANLSMLCGNVGIEGGGVNPLRGQNNVQGACDMGALPN
                     VYPAYQQVANEDVRKKFEGFWNAKLSPKPGLTIMEIMAAAGKGDIKAIYIMGENPMLS
                     DPDLHHVKKELQKLDLMIVQDLFLTETAELADVVLPVASFAEKDGTFSNTERRVQRVR
                     KALDAPGEAKADWEVICGIANKMGYEMNYASAREIFEEVAKVTPSYAGISYERLEKGG
                     IQWPCPTPEHQGTKFLHKDKFSRGLGLFTAIEYIPPDELPDKQYPFLLSTGRVLYHYH
                     TGTMTTRTKGLTERYPESLVEINPADAEKMGIAEGQKVKVTSRRGTVEAKATITKKSA
                     PGSIFMNFHFADASVNLLTNPALDPIGKIPEYKVCAVKVEAA"
     gene            9494..10660
                     /locus_tag="PLR13_08440"
     CDS             9494..10660
                     /locus_tag="PLR13_08440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQC19405.1"
                     /translation="MAEISVNGFCDPAFDKVKQAFIGNFKKGKERGAAVSIVIEGKTV
                     VDIWAGYADFWKRRPWQKDTLVNIYSTTKGVTAICAHRLIEQGRLDPDKPVSAYWPGF
                     EKNGKEAITVRMLLNHTAGMVAIKKRQPGKALYDWDAMVNALENQSPWWKPGKLGYHA
                     ITFGWLVGQVIRNITGMSVGQFLKKEITGPLDLDIHIGLDEAEHHRSANMVMVRIPTI
                     HPDCLKLTRAIVLNPFGATSMALGNPFSIATGVNSRSWRSAEIPSANGQAAARALAKL
                     YGILANGGKAGNIQVLSPGEIDRCSEETSGGDDAVLKLKTRFSLGFMLNQDNPSGNMG
                     PGKKTFGHPGAGGSLAFADPDAHLGFGYVMNKMDTFILVDPRAKRLIKAVYACL"
     gene            complement(10848..11726)
                     /locus_tag="PLR13_08445"
     CDS             complement(10848..11726)
                     /locus_tag="PLR13_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HQC19406.1"
                     /translation="MTQEQTSEIKNIIDRALEQNPENKEIIKAFAPIIIRQRQLAEKP
                     SEQKLDCLAIDKQKLKAGVPVSRQINLILPGDPLKEIALSLAEAVKEGMPQLTENIDL
                     IAGLIKDDKIKPADYFKSTPDGESMATDVWAKDLKVSPSNASFLMSLVSRVILEQRAN
                     EITAAIGAFDWEKGYCPICGEFPSIALIEEEGGKRFLHCSACGYYWRFTRVSCPYCEK
                     ETRQGMEYFYVENKKQESAFVCDQCKKYLVTLYRAGQVFARDMDVSAISLIHMDLLMQ
                     NKGYEPMTACTWNVLS"
     gene            complement(11926..12573)
                     /locus_tag="PLR13_08450"
     CDS             complement(11926..12573)
                     /locus_tag="PLR13_08450"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQC19407.1"
                     /translation="MKSKMVMVTSKYERFVHWLLAISCLLLCYTGLGIMYKELNFLGF
                     IFGGLKGLATVHDIMAVVFAISLVLAIAMWWKEAGIFVFPEDWEWIKAAGGYLWHLDH
                     VPETGKYNPGQKAFFLTVALFGLLMICTGFFMWYPHVLPAALMDWVYVLHVLGFVVIF
                     AFFFVHLYLGTIGNPGSVSAMISGKMELPVLRMLHPKWVKEMEHEGKLMIADDKK"
     gene            complement(12575..13378)
                     /locus_tag="PLR13_08455"
     CDS             complement(12575..13378)
                     /locus_tag="PLR13_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC19408.1"
                     /translation="MGNELVKLIDTTLCTACRGCQVACKQWNEMPGFRTKQTGTYQNP
                     PDLQWNTWTLIRFQEYEDKDENFQWIFRKDGCMHCTDASCVKVCPSGALYYTDIKTVG
                     IHRDKCIGCKECIAACPFDIPRYDKKTEKIYKCDLCYSRIKENLPPACVKSCPTGALT
                     IGPKDEMIKKAYARVQQLGGDANVYGDKFVGGTHVIYVLQFKPEVYDELPQDPKVPVS
                     TFIWKDVLKPLSLLAAGGVIGGSLLHYFTHGPHKPHPEHYDQDTKQGGA"
     gene            complement(13391..>15795)
                     /gene="fdnG"
                     /locus_tag="PLR13_08460"
     CDS             complement(13391..>15795)
                     /gene="fdnG"
                     /locus_tag="PLR13_08460"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HQC19409.1"
                     /translation="ADVILIMGSNAAEHHPIAFKWIMRAKEKGATVIHVDPRYTRTSA
                     RCDFHVPLRSGTDIAFLGGMIKYILENNKYFKDYVVNYTNASFLVNKDFSFNDGLFSG
                     YDSQKRKYNKDTWTLDKDANGIPKRDMTLSDPRCVFQMLKKHYSRYTLDKVSSITGVS
                     KDNLLKVYKAYSATGAPDKAGTECYALGWTHHTVGVQNIRSMAIIQLLLGNMGIAGGG
                     INALRGEPNVQGSTDHCILYANLPGYLKMPAASLDTLEHYLKKCTPESKDPQSANYYS
                     NYPKFFVSFLKSLWDDKVTKENDFGYDWLPKIDDGKHYSTMHMFDAMYEGKIKGYFCV
                     GADTAVSTPNSNKVRKAMENLDWLVGENIFNNETYEFWRGPGVDPAKIKTECFLLPAS
                     ASMEKEGSQSNSGRWVQWKYKAAEAPGDAIPVGEIEIKIMAEVKKLYQKEGGPNASAI
                     TNLKWDYLDSKGHMDVLKVSHQVNGLFTKDTVIEDKAKGTSTLFKKGQCVPTFGNLKD
                     DGSTACGNWIMAGSFDEKGFNKMTKRGKDDPTGLGLFPNWSFAWPVNRRIIYNRASCD
                     IYGKPYNPKRNILEWVGDKWVGDVPDGPWPPQADKAKGKYPFIMKQDGVASLFGPAMA
                     EGPFPEHYEPLESPLAKNPMSGQLNNPAIEIFKGEMDKVASASEKFPYVCTTYSCTEH
                     WCTGALTRWQAWLLEMQPELYVEIGDKLAKEKGIKNGDRVKVSSIRGEAPCVAMVTKR
                     FKPFTVEGKTVHQVGMPFNYGWLFPKDSTSDSTNVLTPTVGDANVFCPEYKAFMVNVE
                     KA"
BASE COUNT         4023 a         3763 c         3811 g         4198 t
ORIGIN      
        1 gatgaaacac ttttgctatt gataccactg atggtcggat gcattcaaca catttaagga
       61 gactccaatc accaaagtga aatgcatcgg aattggttag ttgaactatc caatagctat
      121 gctattggag actatccccg aagcgaagtg gaggggacac tcttcaccct tcacgtttca
      181 cgattcacaa tttgtttaac gttgaacatt gaactatttt ctattcaccc gtttatcggt
      241 ggatatttat atggacattt acggcgattt ccgggtgaca acaacttcgc agcaatcatc
      301 accgcgattt aaatttttgg tgtgagcaac atccacgaaa attttattat caaacagacc
      361 gccgatgagc ccctgatagg tctggacggt gacaataccg caatcggcaa cgccggcctt
      421 ggcaatggct ttctgtgttt cgcaatccgt aatgcgaatg ataattttac catcttcgat
      481 gcaaactttc cgtccctcga tctttaaaac cctgctgaaa atatccaaag catcaaaaat
      541 actttccggc cagacggggt tggggagata gttcttccgg aggttcttgc cgaaaacata
      601 agcaaagtgt ttccaggctt ccacatccat ttcccaagcg gtttgaacac cgaacttctt
      661 cgccagcgcc aaaaaccagg ccccatccat gcgcgtaaac gcgtgctgtg caatttcaat
      721 catctgtgtt tcagttaatt tcatttctaa aaacttcctt tcatctattg ggagtgaagt
      781 ataaggaacg atttcttgtg agtcaagaag gttgtcgcta caacacgctg acgataagaa
      841 gcctattcac tatttaatga gacttgctga aaaaaagctt tgcgatgtaa tcaaacccaa
      901 gttttagatg cgtagcgtcc tgaaacttgt aggttgaatt atcacctatg gtgatttgaa
      961 gcgcaagtcg tagtgacacc cgttgagcgc gagaaaaacg acgcgcaaaa cgttggagga
     1021 actatccaaa agccttcggc tattggaaac tatccccgaa gtgaaacgga gagggggaaa
     1081 agcggaaaag ggggacgccc ttaaaattgt aaaaagggca aaggggtcaa gtctttgctt
     1141 ggctcatgtt ggaaagaaaa agtggattcc caccagaccc ctgcgggaat gacaccttaa
     1201 ataaaaagac atgcctctga aaagaagcat gtctttttgg gtttttgaat tggttaaaat
     1261 ttacgcatct tccactttta ccgcgcagac cttgtattcc ggaatttttc cggtcgggtc
     1321 cagagccggg ttagtcagta ggttgacgga tgcatccgag ttggacaatg gcagaaaagg
     1381 acatcattag aatcaaaact ttgcccttga caaatgtcac gagtcacgtt acacaataca
     1441 catgataaaa acatttgccg acaaacatac gcgtgatttg tttattaccg gtaaatcaaa
     1501 gaagtttcag cctgatattc ttaaaagagc tatcaggcgt cttgaatata tagatttggc
     1561 aacgtgcatt gaagatttaa aagtgccgcc gagcaatcat ctgcacatgc tgaaggataa
     1621 cagagaaggt caatatgcaa tatccgtgaa tgaccagcgg agaatatgtt tccggtttat
     1681 taacggtgat gcatacgatg ttgaaataac cgattatcat taaaagaggt aagaatatga
     1741 gcataaaata tgatgggaaa agaaaagtaa agccgacaca tccgggtgaa attcttaagg
     1801 aagattttat gcctgattat aatcttaatg ttgccactct ggccaaagca ctcggtgtat
     1861 cgcgtcagac ggttaatgaa ttattaagag agagccgcgc tttgacaccg atcatggcgt
     1921 tacgtcttgg ccgtttgttc ggcaatacgg ctgagttttg gttaaacgct cagcgcgctc
     1981 atgatctatg ggaggcgcag cagacgcatc aagctgaaat aaagaaaatc catcctctga
     2041 aagcggctta attgtggggc acattccctg aacgcgccgt atatggatta ttcccaattc
     2101 taatttattg aaatgaaaat aaatggctag cgggacgccc ttaaaattgt aagttactgt
     2161 catattcggg gaacataaaa ctaaaaacac aatgacaatg gtcacaaaat gaataaatca
     2221 aaagataaat tatccgcctt aatcatttat ctttctcctc atggaacaac gaaaaaagct
     2281 attgatacat tggcagtgtc gctaaacgaa aacagcgtga gctctgatat atataatctg
     2341 agtgaatttg ataataacga tattatggca aaaatagcgg actaccagtt gatcgttttg
     2401 ggctcgccga catattttca tcatgcacct accgtgttta ctgattttat caataacatg
     2461 cccgatgcat cgaatgatca gtcagtcgtt ttgctttcct cttttggcgg ggtatccagt
     2521 ggtgttgttt tatatgatct tgctaaaatt ctgcacagga agaaatataa tttgatcggc
     2581 ggcatcagag tcttaacaga acactgcctt acctttcagg aagaaaaacc tttttattca
     2641 gggcatccgg ataagaaagc cttgaacgct atcaagacgt tcgggaaaga aatatctgtc
     2701 cgattgaaaa ataaaatctg tacaagatac agcccttctg atttcaatga taagcctttt
     2761 attctaaact ttatggatga ccacattgtt aagctgagaa attttgtatg ggccatgccg
     2821 ggcataacag taaatcaatc attatgtaac cgctgtgctg tgtgcgttaa aaattgcccc
     2881 acgcataaca taaagctcgg taaagccacc atgcacgggg gaaattgcat ttattgttac
     2941 tgttgtgtca ggaactgccc ggttcatgcg acaatcgcat ttctcaagcc tgcgagtata
     3001 acggtcaaac tgatggcaga aatattccgc aaattcgaag atcaagaaac acagcagata
     3061 gtttgatcgt ttaggagtaa gaggaacgcc gttaaaattc caagttactg gttaaaaagc
     3121 aacaggggca aaggggtcaa gtctgctctt gactcatatt agcggttttt gcgctggttc
     3181 aatcgtcgtc cttcgattga accagaatat ttcggagcaa tcagcaacct gtccggcgca
     3241 tccggcattc tccggagcac ctgccggatg attgcttcgg caatgaaaag ttcatccatt
     3301 gggtgattac cgcttttcat ttttcccacg gcaactacgc ggtgtcaggc ctgtccacga
     3361 tttgaaggcg cgataaaaat tggccacatc acgataacct accttatttg caatttcttc
     3421 catggtaagt tcggttgatt gcagaagatc acaagccttt ttctgacgga tgtctttcac
     3481 gagtgattta taactcacac cctcggccgc taaccggcgc tttagcgttt gatcgctcat
     3541 gttcaaacac cgggcaagct gtgacctcgt gagcagttct ttgtctttaa actgaagcgc
     3601 cgatttagcc aactccgttg ttgttgtaag catgataagc tgctcaagcg tttgacggca
     3661 atctttttca taaatctttt tagtcagcgt attggcgcat ggcaatggtc tggtcaggta
     3721 actgctgtcg aaccgaattt gagtttcttc ggcatcaaag agaatcggac aattaaatat
     3781 tggtttatat tcctctgcat attccggctt ggaataggca aaacggattt ctataagttg
     3841 cactcgctcc cgcataattt cacagcagat attatagccg cctaccatat gcatttcgta
     3901 acggaagcgg ctgctgcgac ccagatcgat gagatcaata aaactgaatt tggcaatatc
     3961 gtcctgaaca tcaaggataa attggaaggg aaatgataag agatcggcaa gctcaaaagc
     4021 cattttaaag gcgtcaaggg ttgtggcaca gctattgata gccattatcc atttccccaa
     4081 ctggccgata ttaatgcgtt ctccaatgcg cagtcctaaa gccgggcagg aaataaggtc
     4141 gaataacttt tgcgagatct caaattgctg ttcaaatgtt atcaatctat ccgggttgtc
     4201 aaaatccgct ttctccagtc cttcactgga gagaatcgac gccgtatcta ttccatgctc
     4261 ttctgcccat tggatgataa cgcgcaagtt atgcgtagat cgatagggcg cgattacagg
     4321 ctttgatttc agagaaattc tcatgattca atcagtatta ttcttcgcca ttaagttgat
     4381 ccaaaatgac aatattttga gcttctccgg caattgtaaa tgatatccgt atcaagtagc
     4441 ataaaacgaa attaaccaag tggtcaataa cacaagcgta gatgaaactc aatgtcgttg
     4501 tcaatgtaaa gtaatatcaa acgtgccgta cactattaaa agaaatcgct gaactggagg
     4561 ctatttatga gaaaaggatt aagactgttt ccgatgatga caatgttgat tttgtttgtt
     4621 ctgacagtgt tttccgttga gatttttgcc tggccggtca taccgccgcc aaactttacg
     4681 aatgaaccgc cccgaaatcc gtttatcgct gattctccct ggccgatgag tcatcgaaat
     4741 ccttacggtc aggcatcctc tccgtatccg ggacctaaat caatcaattt aacaacaaga
     4801 tcatcttttc atgtcgggat gcccggtttg attacgcttg ccatctctgg aaaatatccg
     4861 gatggtaaac gcgtgatttg gggcagttca tcaacggatg tgttcaaggc ggatgaatcg
     4921 ctaaattaca tcaaaactat gaaaaaagaa aatttgacca tttcatctat atttacaccc
     4981 ggtgaaatgc tttccggcgc ttatactctg gtggacaagg ataatacatt ctatatgccc
     5041 cggtttacaa aaatatttgc gtttggcgat gcgattgccg gtaatcgcta ttcggatatc
     5101 attattcagg gcgtgtatga aattcccgca aacctgcttg ttgatccggc ggaaaaaatc
     5161 gtgggactaa gcatgacctg ggacggcatg ctgactcttg ccaccagtca tggattgata
     5221 ggggttgttt cccgtttttt tgacgaagca tattactatc gcttccccgc tgacgaacag
     5281 atttccaatt ccatagcctg tgatgagcat ggaggcattt atgtcgttac cgataaaaat
     5341 atgtaccgag tgcagtggac tggcagtgaa ctcacgatag atcctgacaa gggaggatgg
     5401 agcgccggtt atgaaacggg agaagcttcg ggcgtgcggc tcggagcagg ttcaggctcg
     5461 acaccgacgc taatgggaac cggtaatcag gataaatttg tggtgattac agatggtcag
     5521 aatctcatgc accttgttct tttctggcgc gacgagattc ccgccgactg gcagcagatc
     5581 cccggaacaa aaagccggcg cattgccgca caggtgccgg tgactttcgg taaccccggc
     5641 gcgacagaaa cattgtctga acagtctgta tgcgtcaggg gttatggagc agtggttgtc
     5701 aataaccagt tgaaagcgtc actggacaat caagtcatca gcgttctgat gtccggattt
     5761 gcgccaatcg ctccttatgg tgttgaaaaa ttcgtatggg atccatggac acgaacatta
     5821 aagacagcct gggtaaacaa atcggtaagc atccccaatg gaattcccac catgagtcag
     5881 acgaccaact tgttttatgg cgtcggaaaa agattcgggt tttggacatt tgaagccttg
     5941 gattggacga caggaaaatc tgtcttcagc tatccatacg gcattacacc ggccaataac
     6001 agcgcctatg ccgccacgga aatcgggctt aatggcagcc tcttaaccgg aactttaacc
     6061 ggcatggtac tgatgaaacc ttagcctgtc tgccggcatt gggcatttaa gggacataac
     6121 acttaattaa taaaaaaacc ggatttaccc tgacgggcac catggcccgc cagcgcggga
     6181 atgacacttt agagaaagaa agctataccc cttttcagag gcatagcttt ctttgtttct
     6241 attaatattt taaaagctac gcggcttcca cttttaccgc gcaaaccttg tattccggaa
     6301 ttttgccgat cgggtccaga gccggattgg tcaacaggtt gacggatgcg tccgcgaaat
     6361 ggaagttcat gaagatcgaa ccgggagcac tctttttggt aatggttgcc ttggcttcaa
     6421 ccgtaccgcg tcttgaggtc accttgactt tctggccttc ggcaataccc attttttcgg
     6481 catcggccgg gttgatttcc accaggcttt cgggataacg ctcggtcaga ccttttgtac
     6541 gcgtggtcat ggtaccggtg tggtaatgat acaggacgcg tccggtggaa agcaggaacg
     6601 gatattgttt gtccggcaat tcatcgggcg gaatgtattc aatggcggta aacaatccca
     6661 gaccgcggga gaacttatcc ttgtgcagga atttggtccc ctgatgttcc ggcgtgggac
     6721 agggccactg aatgccgccc ttttccaggc gctcgtaaga gatgcccgca tagctggggg
     6781 tgactttcgc cacttcttca aaaatttccc tggcggaagc gtaattcatc tcgtaaccca
     6841 tcttgttggc aatgccgcag atgacttccc agtcggcttt ggcttcaccc ggcgcgtcga
     6901 gcgctttgcg cacgcgctgt acgcggcgtt cggtattgga aaacgttcca tccttttcgg
     6961 caaagctggc caccggcaaa accacatcgg ccagttcggc ggtttcggtc aggaacagat
     7021 cctgaacaat catcaaatcc agtttttgaa gttctttttt cacgtggtgc aaatcggggt
     7081 ctgaaagcat ggggttttca cccatgatgt aaatcgcttt gatatcgcct ttgccggcgg
     7141 ctgccatgat ttccatgatg gtcaggcccg gtttggggga aagcttggcg ttccagaagc
     7201 cttcaaactt tttacggaca tcttcattgg ccacctgctg gtaggcggga taaacattgg
     7261 gaagtgctcc catgtcgcag gcgccctgaa cattgttctg acctctgagc ggattcacgc
     7321 cgccgccttc aataccgaca ttgccgcaca acatggaaag attagcacag ctcttgacgt
     7381 tgtcggttcc ggtaatgtgc tgggtaatgc ccatggcgta aaggatgctg gcacggtctg
     7441 ccttggcata catccgggcg gctttcttca aatcttccgc gggcacgccg gaaatctttt
     7501 cgaccttttc cggtgtgtat ttttcgacca tcttcttgag cgcgtcgaat ccttcggttc
     7561 tgtctttcac gtattccttg gcataaagac cttcggagat gatgacgttc atcattccgt
     7621 tgataacggc aacatcggtg ccgggattct ggcgcagcca gatatcggcg tatttgacca
     7681 gatcgatttc gcggggatca atcacaatca gtttcgcgcc gcgcttggtt gcccgtttga
     7741 ccttggcgcc gatcaccgga tgattttcgg tggtgttggt gcccgttgcc agaatcaggt
     7801 ctgtgaattc aatttcgtct atagagtttg tcattgcacc gctgccgaat gtggcggcca
     7861 gaccggccac cgtgacagag tgtcagagac gggcgcagtg atcaacattg ttggtgccga
     7921 ttcccgcccg caggaatttc tggaacaggt aattttcttc attggtgcag cgcgccgagg
     7981 cgagtcccgc aatgctgtcg gcgccgtgtt cttttttgat cctggaaagt ttgtccgcga
     8041 tgaaggtaaa ggcttcatcc caggtcgctt ctttgaagtt ttcattcttt ttataacgga
     8101 tgagcggtgt tttcaggcga tcgggatggg aaagaaaatc catgccgaaa cgtcccttga
     8161 cgcacaggct gccgtcattg ggctggccgt attcgcggtt gcccatgacc ttgacgattt
     8221 tattatcctt ggtgtagaga tccatctgac aaccaacgcc gcaataggtg caggtggttt
     8281 tgacttttgc aagttcccac ggacggccgg cgaatttcgc ctgtttgaat gtgagagcgc
     8341 ctgtgggaca ggcctgcacg cactcgccgc agaagacgca ggttgaatca atgtaatcct
     8401 ggtcgaatgc ggggccgacc ttggccttat aactgcggtt ggcaaaatcc agaacttcgt
     8461 tgacctggat ttcattgcag gcgcgcacgc agcgtccgca gagaatgcat ttgttcatgt
     8521 cgcgcgcaat catggtattg ctgtcatcct tcgcataacc cggcacttga atttcaaagc
     8581 gcggcttgtc aatctgcagc cagtaaacca gattctgcaa ttcgcaatta ccgttctgct
     8641 cgcaggtgag gcagttatga tctccggacg accagagcag ttcgatgatc attttctggg
     8701 ccttgcgaac ggcttccgta tcggtgcgga caaccatatt gggcgtcacc ggcatacagc
     8761 aggatgcggc aagagatcgg gcgccttcca cttcgaccac gcatacgcgg caggcaccga
     8821 cattggttgt tttggtataa tgacaaaggg tcggaataaa aatgccgtta tcccgtgcgc
     8881 attctaaaat tgtctgaccg gcagtcgctt caacatcttt tccgtcaatt ctgataggta
     8941 tttttttctc catgtctttc tccaatctta aattatctga actcttactg accaagagtt
     9001 atattttttt taacatcgat ttcataacga caagcaatcg ggttgtcaat tctgtcaata
     9061 ttgacttatg ccttttttca cataggccaa aaatgacaat acgttaaata tatcaaatac
     9121 ataatgcaat gccgctaccg acgtccgccg taaataaatt gggcgcaacg ggtggaaatg
     9181 cccgaagcct gactggactt gtcagacatg gtccggtgag gatgggacgt gatgtttctt
     9241 ttgcctatgt tgttgcaaca gcaaaagcgt tgcctgtgca aaatgatgca agggttttat
     9301 cgtcaggagg agtatcgtta caggccatca tagtcgttga caggcaatca ggcattgatg
     9361 tagcttgcaa aagcgtgtag ctccgaaaaa gggcgtgcat caggtcagtg cacggcaaca
     9421 cacttatgcg gttgatttgt tctgcaaaaa cgaatcagac gaagaatgat cgaatgtaat
     9481 accgggagat aatatggctg aaataagtgt caatggtttt tgtgatccgg cttttgataa
     9541 ggtgaagcag gcgtttattg gcaattttaa aaaaggcaag gagcggggag ctgccgtttc
     9601 aattgtcatc gaaggcaaaa cggttgttga catctgggcc ggttatgcgg atttctggaa
     9661 gaggcggcca tggcaaaaag atacgctggt caacatctat tcgacgacca agggcgttac
     9721 cgccatttgc gcccaccggc tgatcgagca gggacggctg gatccggata agccggtgtc
     9781 ggcgtactgg ccgggatttg agaaaaacgg aaaagaagcc attacggttc gcatgctgct
     9841 gaaccatacc gccggcatgg tcgctataaa aaaacgccaa ccgggaaagg cgctgtacga
     9901 ctgggatgcc atggtcaacg ctctggagaa ccagtctccc tggtggaaac ctggcaagct
     9961 cggctaccac gccattactt tcggctggct ggtggggcag gtcattcgaa atattaccgg
    10021 catgtccgtg ggacaatttt taaaaaagga aattaccggt ccgctggacc tcgatattca
    10081 tatcggtctg gatgaagccg agcatcaccg gtcggcaaac atggtcatgg tgcggatccc
    10141 cacgatccac ccggactgtc tgaaattaac cagggcgatt gtcctgaatc cttttggcgc
    10201 aacatccatg gccctgggca atcccttcag tatcgccacg ggcgtcaaca gccgttcctg
    10261 gcgaagcgcc gagattcctt ccgcaaacgg ccaggccgcg gcccgggccc tggcaaagct
    10321 atacggtatt cttgccaacg gcggcaaagc gggcaacata caggtcctgt cacccggtga
    10381 aatagaccgg tgctccgagg agacctccgg cggcgacgat gccgttttga aattgaaaac
    10441 gcgattcagc ctgggcttca tgctgaatca ggataacccc tcgggcaaca tgggccccgg
    10501 caaaaagaca ttcggccatc ccggcgcggg cggctccctt gcctttgccg atccggatgc
    10561 gcatctggga ttcggctatg tgatgaacaa aatggatacg tttattctgg tcgatccccg
    10621 ggcaaagcgt cttatcaaag cggtgtacgc gtgcctgtga cggtttcatt gtttcaatca
    10681 gaaagctgat taccgcaagt cccctgcaac ggcatcagtt ggtgatcatc agaaatattg
    10741 tatcgacaac tgccggaatg cgaaaaaaca attcttcaga aagaagcaaa tacaggacgt
    10801 gatgcaagct gccgggtcaa tttctgtttc gggcgatggt caattgatca gctcaaaaca
    10861 ttccaggtac aggcggtcat gggttcgtag cctttgttct gcatgagcag atccatgtga
    10921 atcaggctga tggcggaaac atccatatcg cgcgcaaaga cctggcccgc ccggtaaagg
    10981 gtgacaaggt attttttaca ttggtcacag acgaaagcgg attcctgttt tttattttcc
    11041 acatagaaat attccatccc ctgccgggtt tccttttcac agtaaggaca actgacccgc
    11101 gtaaagcgcc agtaataacc gcaggcagag caatgcagga agcgctttcc gccctcttcc
    11161 tcaattaacg caatggacgg aaattctccg caaatcgggc agtacccttt ttcccagtca
    11221 aaggcgccga tggcggccgt tatctcgttg gctctctgtt ccaggataac gcggctgacc
    11281 aggctcatta aaaatgacgc attggacggc gagactttta aatctttcgc ccagacatcg
    11341 gtggccatgc tttccccgtc cggggtgctt ttgaaataat ccgccggttt gattttatcg
    11401 tctttgatca gccctgctat cagatcgata ttttccgtca gttgcggcat gccttccttc
    11461 accgcctcgg ccagcgacag agcgatttct ttcaacggat cgcccggcaa tattaaattg
    11521 atctgacggc tgaccggcac gccggccttg agtttctgtt tatcgattgc caagcagtcc
    11581 agtttctgct ctgaaggctt ttcggcaagc tgtctctgcc ggatgataat cggtgcaaac
    11641 gctttaatga tttccttatt ctcaggattc tgttccagag cgcggtcaat gatatttttg
    11701 atctcggacg tttgttcttg ggtcatgatt tttcctttta ctaattagtc ccccatagtg
    11761 tttcatgcgg gattgtttcc gatagccaaa agctactatc gatggtcaac gaatctcaca
    11821 aaagcgagcc tcaaaacaaa agtggcgcac accttctatc agatgtgcgc cacccggtca
    11881 attaaatatg aaccacatcg tttccttgcg ggaaattgtt caatcttact ttttatcgtc
    11941 ggcaatcatg agtttgcctt catgctccat ctctttgacc catttcggat gaagcattct
    12001 cagtaccggc aactccatct tcccgctgat catcgcggaa acagatccgg gattaccaat
    12061 ggtccccaaa tacaggtgga caaagaagaa cgcaaagatg accacaaagc ccaacacatg
    12121 caggacataa acccaatcca tcaatgccgc cggaagtacg tggggatacc acatgaaaaa
    12181 tccggtgcag atcatcagta atccaaacaa ggcgacggtc agaaagaacg ccttctggcc
    12241 gggattgtat ttgccggtct ccgggacgtg atccagatgc cacaggtaac cgccggctgc
    12301 cttgatccat tcccagtctt ccgggaaaac aaatatgccc gcttctttcc accacatcgc
    12361 tatggccaat accaacgata tggcaaaaac cacagccata atgtcatgaa cggtggccaa
    12421 tcctttcaaa ccgccgaaaa tgaaacccaa aaagttcaac tctttgtaca taatccccag
    12481 tccggtatag cataaaagaa ggcaggagat ggccaacagc caatgaacga atctctcata
    12541 ttttgatgtc accattacca ttttactttt catcttacgc acctccctgc ttcgtatctt
    12601 gatcataatg ctccggatgg ggcttgtgcg gaccgtgggt gaagtaatgt aataatgatc
    12661 cgccaatcac gccgcccgcg gccagcaggc tcagaggttt gagcacatct ttccagataa
    12721 aggtagaaac cggtactttc ggatcttgcg gcagttcgtc ataaacttcc ggcttgaact
    12781 gaagcacata aataacgtgt gtgccgccta cgaatttatc gccgtacaca ttggcgtcgc
    12841 cgcccagttg ctgtacgcgg gcataggcct ttttaatcat ctcgtccttg ggcccgatgg
    12901 tcagagcgcc ggtcggacag gatttgacac aggccggggg aaggttttcc ttaatgcgcg
    12961 aatagcaaag atcgcatttg taaatcttct cggttttctt atcgtagcgg ggaatgtcaa
    13021 acggacaggc cgcaatgcat tccttgcagc cgatgcattt gtcgcgatga atacccaccg
    13081 tcttgatgtc cgtataatac agagcgccgc tggggcagac ttttacacag gatgcgtccg
    13141 tgcagtgcat gcaaccgtct ttgcggaaga tccactggaa gttttcatcc ttgtcttcat
    13201 attcctgaaa gcggatcagc gtccaggtgt tccactgcaa atccggcgga ttctggtacg
    13261 tgccggtttg ttttgtcctg aagcccggca tttcattcca ctgcttgcag gcgacctggc
    13321 aaccgcggca ggcggtgcac agggttgtat caatcaattt tacaagttca tttcccatgg
    13381 tttatcctcc ttaagccttt tccacgttga ccatgaatgc cttgtactcg ggacagaaga
    13441 cattcgcgtc accaacggtt ggggttaata cattcgtgct gtcactggtg ctgtccttgg
    13501 ggaagagcca gccgtaatta aagggcatgc cgacctgatg gaccgttttt ccttcaaccg
    13561 taaaaggctt gaagcgtttg gtcaccatgg ccacgcaggg cgcttcgcca cgaatggagg
    13621 aaaccttaac gcggtcgcca tttttaatac ctttttcctt agccagttta tcgccgattt
    13681 caacgtagag ctccggctgc atttccagaa gccatgcctg ccaacgggtc agcgcgccgg
    13741 tgcaccagtg ctcggtgcag gaatacgtcg tgcagacata ggggaacttc tcgctggcgc
    13801 tggccacttt atccatttca cctttgaaga tttcaatggc cgggttgttg agctgtccgg
    13861 acatcggatt tttagccaga gggctttcca gcggttcgta atgctccggg aaaggacctt
    13921 cagccatggc gggtccgaac aaagatgcca caccatcttg tttcataata aaaggatatt
    13981 tgccttttgc cttgtccgct tgcggcggcc agggaccatc aggcacgtct ccaacccatt
    14041 tatctcccac ccattccaga atattacgtt tgggattgta cggtttgccg taaatatcgc
    14101 aggaagcgcg attgtaaatg atgcggcgat tgaccggcca ggcgaatgac cagttgggga
    14161 aaagccccaa accggtggga tcgtctttgc cgcgtttggt cattttgttg aaaccttttt
    14221 cgtcaaaact accggccata atccagttac cgcaggctgt ggatccgtca tccttgaggt
    14281 ttccaaaggt cgggacacac tgtccttttt taaacaaagt ggatgtacct ttcgccttat
    14341 cctcgataac cgtgtctttt gtaaagaggc cattgacctg atgggaaact ttcaacacat
    14401 ccatgtgccc cttgctgtcc agataatccc acttgagatt ggtaatagcc gaggcattgg
    14461 gaccgccttc tttctggtaa agctttttga cttcagccat aatcttaatt tcgatttcac
    14521 ccacgggaat ggcatcgccg ggagcttcgg cagctttgta tttccactga acccagcggc
    14581 cggaattgct ctgcgatcct tccttttcca tactcgcgga agcgggaagc aggaagcatt
    14641 ccgtcttgat ctttgcagga tcgacaccgg ggcctctcca gaattcgtag gtttcgttgt
    14701 tgaaaatatt ttcacccacg agccagtcca gattttccat cgccttgcga accttgttgc
    14761 tgttcggcgt gctgacggcc gtatccgcgc caacacagaa gtatcccttg atcttgcctt
    14821 catacatcgc atcgaacatg tgcatcgtgg aatagtgttt cccgtcatca attttgggaa
    14881 gccagtcgta gccgaaatca ttttcttttg tcaccttgtc gtcccacagg gattttaaga
    14941 aactgacaaa gaatttgggg taattagaat agtaattggc cgactgcgga tccttggatt
    15001 ccggcgtgca ttttttcaaa tagtgttcca gcgtgtccag agaagccgcg ggcatcttca
    15061 gataaccggg cagatttgca tataaaatgc aatggtctgt cgagccctga acgttgggtt
    15121 cgccgcgcag cgcattgatg ccgccgcctg cgatacccat attgcccaaa agcaactgaa
    15181 taatcgccat ggagcggatg ttctgtacgc ccacggtatg atgtgtccac cccagagcgt
    15241 agcattccgt accggctttg tccggagcgc cggtcgcgct gtaggctttg tacaccttga
    15301 ggaggttgtc cttggatacg ccggtgatgc tggaaacctt atccagggta taccgtgaat
    15361 aatgtttttt cagcatctgg aaaacacagc ggggatcaga cagggtcatg tcccgtttgg
    15421 gaataccgtt tgcatcttta tcgagagtcc atgtatcttt attgtacttg cgtttttgcg
    15481 aatcgtaacc ggagaaaaga ccgtcattaa aagaaaaatc tttgtttacg aggaacgacg
    15541 cgttggtgta attgactacg tagtctttga agtatttatt gttttccaga atatatttga
    15601 tcataccgcc cagaaaagcg atatcggtac cggagcgaag cggcacatgg aaatcgcagc
    15661 gcgccgatgt tcttgtataa cggggatcga catggataac cgtcgctccc ttttcttttg
    15721 ctctcataat ccacttgaaa gcgatggggt gatgttccgc ggcattgctg cccataatca
    15781 aaatgacatc cgcgt
//
LOCUS       DAONQZ010000051        15633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4196_length_15633_cov_15.869560, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000051 DAONQZ010000000
VERSION     DAONQZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_15633_cov_15.869560"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLR13_08465"
     CDS             complement(<1..283)
                     /locus_tag="PLR13_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19410.1"
                     /translation="MKKLLLSVIAVVMAMMPVASFAKAVISDSDLDSAKAEKSVSIAL
                     AEKVIADSELDEVTAEAGVSISFVDVKIKDVASSATLSIGDSDGFTGYTG"
     gene            complement(478..954)
                     /locus_tag="PLR13_08470"
     CDS             complement(478..954)
                     /locus_tag="PLR13_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19411.1"
                     /translation="MKKVLLFAVLAMFLMVPMSFAKTAISESDLAAVTAQEGVTINFD
                     CFTVGAISIDVQSWGDSDGCAGCGGYTGEGWVGASIEMSTDFVNIKGNLTIDVGTSGT
                     QTALIIGLPTLDLAGSMTQTVKLGTAANLSGAAVMGTAYMEGLSVSPTGKLIIYAH"
     gene            complement(1182..1940)
                     /locus_tag="PLR13_08475"
     CDS             complement(1182..1940)
                     /locus_tag="PLR13_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19412.1"
                     /translation="MKMNLKQTRVRPLFFKIFCALFAVTLFVAFSSSTGYAPTQMSDS
                     ELSEIEGQAFFKIEHFPASSEFWDVVDPSGDPVGRKYNTGSQNVIRISMGIDVEAHAF
                     IESQKMGYYNGGWDQDHTNFWVGDRSSTGDGGPLRLNGLYIDLGYDNIGNAANRTLNY
                     VEIGSMRVTGNVTQTINSINGLIAAGTGANNGVMLRQTASGTRVVNFNNEIMSFVFAS
                     KYNYESNVGSTNTVVSGIFLKIPSYHSGSELQRP"
     gene            complement(2142..3008)
                     /locus_tag="PLR13_08480"
     CDS             complement(2142..3008)
                     /locus_tag="PLR13_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19413.1"
                     /translation="MRTKLLFPLLVFVLLFCAMPVDAKTIVKIGRDVDISEDQQATGV
                     IAVGGQITVRGLVEKNVVAVGGSVVITGDAVVRGNVLCIGGVVVRGSGARIFGNVTEV
                     NSANFLAAVSSAFYEEEEEWSWLTDIIYFCFFTFLLMLALMLTFLFPRPLNAIIDSVA
                     AQKTKSFFWGILGALMIAPLFMLLALSFIGIPLIPLAFSLVLLAFIFGFLAMSALLGR
                     FVLTNFFRHHKLSLMKEALLGMMLWWVIGWAPFYTGMMIKAAVITMGFGGVLVALIDR
                     GRNRQKTAHQAF"
     gene            complement(3021..3674)
                     /locus_tag="PLR13_08485"
     CDS             complement(3021..3674)
                     /locus_tag="PLR13_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQC19414.1"
                     /translation="MDVLFHLIDFFLHLDKYLPLIIQSFGIWAYVIMFLVIFCETGLV
                     VTPILPGDSLLFALGAFAAQGALNIEILLISLCIAAVAGDTVNYSIGKFLGPKVFHYP
                     DSRFFKREYLVRTHQFYEKHGGKTIIIARFIPIIRTFAPFVAGIGTMKYLRFISYNVV
                     GGISWICLFLLAGYYFGGIPAVKRNFTLVIIAIIIISVMPGVIEYVRQKYFVSRAKN"
     gene            complement(3825..5318)
                     /locus_tag="PLR13_08490"
     CDS             complement(3825..5318)
                     /locus_tag="PLR13_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19415.1"
                     /translation="MRLACVFNPFQYKLHEENLRVVQKYFGLFPPLSLAWVCAIAEKA
                     GHDALLIDARTLRLTREDVLDRLKQWKPDILGFMMTTYMFRETLEWVTFLKKHLKLPV
                     IIGGYNLRVYPNESLSVEGPDFGCFNSAFYTLPRLLEELEGRRRFADVPGLIYKENGR
                     TTMTPYGRDPVFDDYPNPARHLLPNDLYAEFTTERKNFTVMVTSKGCPNGCLFCEAGH
                     TSYNPRSPQTVLGEIKECRTRYGIREIDFFDYDFCIDRKRTEAICDGIIQNGMDITWA
                     CRTRVDCVDEALLRKMKAAGCSRIYFGIESGVQEILDRLNKGISLQQIRDTIETTRSL
                     GIRALGFFLVGSPGETRETFQQTVKFAKSLKLDYVQFSKLTAKPLTPYWRQAVDQTGV
                     DYWREYILGNIEDKSLARPWTALANDDIDRLAKKAYVTYHLRPMFLLRHTLQVKSFGE
                     FKRKFYAFLEMMLRQEPIGTLDENFVAYPEDMGKLCFYNQNFKKNKP"
     gene            complement(5408..6391)
                     /locus_tag="PLR13_08495"
     CDS             complement(5408..6391)
                     /locus_tag="PLR13_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQC19416.1"
                     /translation="MSIQKHHVWEYPTLSTPKAILRFVFTWLVTLAIFVVLFSRIPFH
                     DVVSLLQQTDMRFLCAGLLCSVLAHLFFSSARFQRVVKMTGCRISFGEAVIIRMGCNP
                     IKGILPFKMGEFAILGYMKERHHLSYARGLLALLSGYMFSFTALIFFCSMGGLFYVHP
                     PLQKMLFAAACLLIVLLIAISIRQNFFRPVVLYIHKILRLPENVRKIIESYDPQTGQA
                     IMIHSLAIEGCKLLIILFSFKSLSIDLPVDALLLWGSMTIIAAYLPMTYWGLGMRESA
                     VIVLFAGYAAPGHLLAAGLLMTFVDGLLPVFLGLFFIRPFLNNWLGSMMQK"
     gene            6596..8644
                     /locus_tag="PLR13_08500"
     CDS             6596..8644
                     /locus_tag="PLR13_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19417.1"
                     /translation="MNLSSDRQRLIVYVVLIALSLFVFSQVGQFDFITFDDPVYVVDN
                     PSIQSGFTMDGVIWAFGTIHAEFWHPLTWLSLMLDYQLFGLNAGGYHITNLMLHILST
                     LMLFWLLHRMTGAVWRSAFVAALFAIHPLHVETVVWIAKRKDVLSAFFWMLTLCLYVY
                     YTEKPMVKRYLFVFLSFACALMSKPMAVTLPVIMIVLDYWPLKRFASKKENPVFWQVK
                     EKLRFFMLSAVFSLITIFAQYDKYIKHAETSFDSRLANAPVSLTTYLAKTLWPYDLAV
                     FYPFSYQLPAWQTAGSIALILAISVAVILAAKRLPYLLVGWLWYVLAVLPVLDIFRSS
                     ARAMSDNYTYLPAVGLYVMAAWGIPDLFCQKQIRRKILWPAGIAVAAMLSVLAWQQCS
                     YWQNSFKLFNHALHVTKDNYLAHNQRGNVYSKSQQYPPAIADFNEAIRIKPDYAFAFN
                     NRGNTYLQLGRYQQAISDYSEAIRLNPDYARAYYNRGFTYGIHMGRYQRAIDDLNEAI
                     RREPDAPDIHLTRGIVYSNLGGQELACLDYQKACDLGNCKLLDMALGKGECLAADLQP
                     HDSGYYHNSGISYFKRGQYRIALGYFTHAIHFKKDFANAYHNRGTTYSELGRYQHAVA
                     DFNESIRLNPGYPDAYKNRGIAYLLQGEKEQGCSDFKKACALGDCKLLELAESKGDCR
                     "
     gene            8772..10223
                     /locus_tag="PLR13_08505"
     CDS             8772..10223
                     /locus_tag="PLR13_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19418.1"
                     /translation="MKRPKILLFKSHSIVSKVTGVTPPLGLLYIAAALRERLGADVRV
                     IVAMLENDPMNVMVSTLRHFRPDVVGISALTAEAFFAHKAAAAVKAEAPSVPVIMGGP
                     HASCDPELVLQDSCVDAVVMGEGEDTFTELVSVTMSEKSVWQNPDILQKIDGLAFRVD
                     GKMKLSKPRAPINDLDALPFPAWDLIDYKKYWTLPKMATDGVRPYLTMFTSRGCPYNC
                     IYCHQLFGKSFRSRSPESVVEETARLMRMGAESIEILDDISNFNPERFDRMLELMLEK
                     NLHPVLSFPNALRADLIRKESADLLKCVGTGEISVAIETASDRLQKLLGKNLSLEKAK
                     RTIDWFADRRIFTRGFFMLGFPTETQDEMLATIRFAHASRLHLALFFTPNPFRNTALY
                     DLFVDTGKLPSTSTSIDYEYYGAPFNGSDVSDRRYRMLYRWAYYGFYFNPVRLWRIAQ
                     DRPTLADIPASAWTRLNNVVSFRRLKEEDTSLV"
     gene            10307..11413
                     /locus_tag="PLR13_08510"
     CDS             10307..11413
                     /locus_tag="PLR13_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19419.1"
                     /translation="MNSLFDLSNLYGLACNIWPRFPYDIPQLQTALKPFLYLFNITNQ
                     CNLNCYYCCQKTSLTRNRPELSFEDIAGIIKKLPRYAVIVFSGGEPLCRDDFTQLLQE
                     TRRLKRKTALLTNGLLLNDDIICEMIQNRLLNIGIAIDGDKYYYEKIKGRGNYAVVMD
                     NVDRLAYHKKKAKSSFPAFDWKVTVFPENVSRLPLLYQQAADHGADSFTVSFPKKNDF
                     QFNDCLHDIAVLDCEPVSNGNYRFPENIEDIYKELFSIARTSRTRLRTYPQMKQAEDL
                     KNYFETSAIQKRYSACRMPWSGMVISSTGEAYPCLSVRIGDLKKQSLAQVLTSPENIR
                     FRNQLKKRSLFAICDGCCYARLKPVLKRERAVGA"
     gene            11406..12875
                     /locus_tag="PLR13_08515"
     CDS             11406..12875
                     /locus_tag="PLR13_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19420.1"
                     /translation="MRKILFIYPRLGYMDAQRSKPAPPLGILAAVSFLKDTYEIIILD
                     QRLFASDDSFYAKLYNVLQNQPLYAGLSVYSGGMIKFALEISRYIKEQSSVPVVWGGI
                     HPSLLPEQTLENQYIDYVIQGEGELTLPEFSDRLVQYGQNMPPVRGVWIKKQGKIAYG
                     GDRDLIDLQAAPVIPYDLVDFNNYVQYYNGHKYAYYQASRGCPRHCRYCYNHVFNKGK
                     FRAKSADKIISEIKTLAERYASFTGIFFVDDNIFAMGKDYVMNLGKGLGELGLRWAVQ
                     GSDIIALKDYTESDFKRLETCGLTRLTIGVESASKKMRTLIGKQGSPADIEQVMARLS
                     GTKILIWCSYIISLPGETLDDLRASIKLIFRLQHINPNVRNSPFYMYIPFSGTPLYEQ
                     YKDVFPGPESLEEWGQVGWEREHTNSFADYLKDTHFFQSLFLTSLLDDDKVSDFSKNK
                     LLVFLAKCYRPVARWRLKNLFFKFNIELSMFKKFFPDIF"
     gene            13140..15041
                     /locus_tag="PLR13_08520"
     CDS             13140..15041
                     /locus_tag="PLR13_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19421.1"
                     /translation="MAGKSISQLQNASGFLIQFLRRKSDIAALILIAAVIALPSISPL
                     SSGSKVNFNDDFFYYAGMHESVRQAVLEHHTFPTRSLWIGGGYPSLGNPEDPALNPLT
                     LITIVFGSVMALKIITFMALLVGGLSSYLLARHILGYTKWGSLFCGLVFGLSLFVPLR
                     VYDGNTNEVYVAFLPLCMLLIGLACRGRKTAVFILAFVFYTMLSDGKLIALMIFLYLG
                     ILCLLDIIPSFNIFATKNLNKMNIKPLKVLFLALTITFFVGMLRILPVLDMIETMGGL
                     QSNFLWFKPITYSPSGVVTLQELWHNLIGYKDILNIATIGLIPVLLALSVLFIFPPKA
                     FPWAVTIFLFTWLMLAYNAPVDLSKLLWHLPVFNIITKHKYYAFPIVFSLIIVAGQFF
                     WLLSKIRSKWLEHALAAALILLSVWFLYPRFDAIQKNTYTYDIPEEFLVRQNEFYNIQ
                     GLGIPRFRAKPLNSVIYTNVIRNVGIIDSVTHYGEKAVPKYFVDAEGTLIPNPGYKGE
                     AHFADPANTAKATFRPNSIMVQVNLRKPDMLVINQNYERNWHSEHGRIFNKGGLIALR
                     LDKPGVYDITLRYFFRSFFVGLFLSIVSLIALISICWSYKTGRLIKWSHDAPIHIRWM
                     PRFILWLIN"
     gene            15120..>15633
                     /locus_tag="PLR13_08525"
     CDS             15120..>15633
                     /locus_tag="PLR13_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19422.1"
                     /translation="MDTGLQRQKVFIYIFLTVVSLAVYGQVGQFDFINFDDNVYITDN
                     HHVQSGITLASIRWALTTTYAEFWHPLTWLSLMADYELYGLHAGGYHVTNLIFHILST
                     LLLFWLFHRMTGALWKSAFVAAFFALHPLHVESVAWVSERKDVLSAFFWMLTLCLYVY
                     YAEKPVIKRYA"
BASE COUNT         4191 a         3817 c         3550 g         4075 t
ORIGIN      
        1 cgcccgtgta accggtaaag ccgtcggaat cgccgatgga cagcgtggcg ctggaagcaa
       61 cgtctttaat ctttacatca acgaaactga tactgacacc cgcttcggcg gtcacctcgt
      121 ccagttcact gtcggcaata accttttcgg ccagcgcgat actgacgctt ttctccgctt
      181 ttgcggaatc aagatcgctg tcagaaataa cggctttggc aaatgatgca accggcatca
      241 tcgccataac gaccgctatc actgacagta aaagtttttt cataatcttc cctttaaaat
      301 tttccgggaa aagcaagtta cgtttcatca atattcatta tggagcaagt aagtttttaa
      361 acaatgcctg gtgccaacgg ctattttttt atgcgtgtga taaagagagc gcgggagtcc
      421 gcgctccctt ttatccacat tcaccggctg aaacaaacgc cgttttaact aaaaatctta
      481 gtgagcgtaa atgatcaact ttcccgtcgg agatacggaa agaccttcca tgtaagccgt
      541 acccatgaca gcagcgccgc tcagatttgc ggccgtaccc agtttgacag tctgagtcat
      601 gctgccggcc agatccagcg tgggcagacc aatgatcaga gcggtctgag ttccggaggt
      661 accgacatcg atcgtcaggt tgcctttaat attgacaaag tcggtggaca tttcaatgct
      721 agcgcctacc caaccttctc ccgtgtaacc gccgcagcct gcgcaaccgt cggagtcacc
      781 ccaggactgt acatcaatag aaatagctcc cacggtgaaa caatcaaaat taatcgtgac
      841 gccttcctga gcggtcacag cggccagatc gctttctgaa atcgcggtct tggcaaatga
      901 catggggacc atcagaaaca tcgcaagaac tgcaaacaac aaaactttct tcatcttttc
      961 tcctcctaga aatttggtca tgcctttgaa aacgtaccat gcgttcaaac ataaacgggc
     1021 tttaaagctg acttcttccg tttaagcccg cacgccggtt tttttctcca gcgggttcaa
     1081 aatacttctg cttctcctct aacaacaaaa ctcacttgct gcttttaacc gcctcgggat
     1141 tttcccgccg gcagctttat tgatattatc atcctcctgc gtcacggcct ttgtaattcg
     1201 ctaccactat gataggaagg aatctttaaa aagattccgg acacgactgt gttggtgctg
     1261 ccgacattgc tttcataatt atacttcgac gcaaaaacga acgacatgat ttcattattg
     1321 aaattaacca ccctggtgcc ggaagccgtc tgcctgagca taaccccgtt atttgctccg
     1381 gtaccagctg caatcagtcc gttaatagag ttaatggtct gtgtaacatt acccgtaaca
     1441 cgcatactgc ctatttcaac gtaattgagt gttcggttcg cagcattgcc gatattatca
     1501 taacccagat cgatgtacag gccgttgagt ctcaaaggcc cgccgtcacc ggtggaactt
     1561 cgatctccca cccagaaatt cgtgtgatcc tggtcccatc cgccgttata gtaacccatt
     1621 ttttgagact cgataaacgc atgggcctcc acgtcgattc ccatggagat gcgaatgaca
     1681 ttttggctgc cggtattata ttttctccca acgggatcac ccgacggatc gacgacatcc
     1741 cagaattcac tggatgcggg aaaatgctct attttgaaaa atgcctggcc ttcaatttca
     1801 gaaagctcgg agtcggacat ctgggtcggc gcataacctg tggatgaaga aaaggcgaca
     1861 aacaacgtta ccgcaaacaa agcacaaaat attttgaaaa aaaggggcct cactcttgtc
     1921 tgcttcaagt tcattttcat cccgaaacgg gctccttaag aaatcataat cagttttatg
     1981 ttttaagggc taatattttt acgagtggtt taccatatac agaccaaaaa taaatgtcaa
     2041 gcgcaaattt ttccggttta tttcataatc atcttattgc tttaacacct taaaaaaaca
     2101 gagccgtctt tcagacaggc gcgaatgtct tttacgggtg actaaaacgc ctgatgagcc
     2161 gttttttgcc ggttgcgtcc ccggtcgatg agggcaacca gcacgccgcc gaaacccatg
     2221 gtgataacgg cagccttgat catcatcccc gtataaaaag gcgcccagcc gataacccac
     2281 cataacatca tgcccagcaa ggcttctttc atcaatgaca gcttatgatg acgaaaaaaa
     2341 tttgtcaaga caaaccggcc cagaagggcg ctcatcgcca ggaatccaaa aataaacgcc
     2401 agaagcacaa gagaaaaagc gagcggaatc aaaggaatgc cgataaagga aagcgcaagc
     2461 agcatgaaaa gaggagcaat catcagggcg cccagaattc cccagaagaa agattttgtt
     2521 ttctgagcgg caacggagtc aataatcgca ttcaacggac ggggaaacaa aaacgtcagc
     2581 atcagcgcca gcatcaggag aaaagtgaaa aaacaaaaat aaatgatgtc cgtcagccag
     2641 gaccattcct cctcttcttc gtaaaaggcg gaagaaaccg cggccagaaa attcgccgag
     2701 ttcacttccg taacgtttcc gaaaatacgg gccccgctgc cccggacaac aacaccgccg
     2761 atacagagca cgtttccccg cacaacggcg tcaccggtga tcacaacgga gccgcccacg
     2821 gcgacgacat ttttttcaac cagcccccgg acggtaatct gaccgccgac ggcgatgacg
     2881 cccgtcgcct gctgatcttc ggaaatgtcg acgtccctgc cgattttgac aatggttttg
     2941 gcgtcaaccg gcattgcaca aaacaacaat acgaaaacga ggagggggaa aagcaacttt
     3001 gttctcatgt tgcaccgttc ttaatttttt gcacgcgata caaaatattt ctgccggaca
     3061 tattcaatga cacccggcat cacggaaatg atgataatcg cgatgatcac cagggtaaaa
     3121 ttccttttga cggcgggaat gccgccgaag taatatcccg ccagcagaaa aaggcaaatc
     3181 caggaaatgc ccccgacaac attatagctg atgaatcgca gatatttcat tgtgccgata
     3241 ccggccacga aaggggcgaa ggtgcggata atgggaatga accgggcgat gataatggtt
     3301 ttgccgccgt gcttttcgta aaactgatgg gttcgcacaa ggtattcccg tttgaaaaag
     3361 cggctgtccg gataatgaaa caccttggga ccgagaaatt tcccgatgga atagttgacc
     3421 gtatcgcccg caacggcggc aatgcagagc gatatcagta aaatttcaat attcaacgcg
     3481 ccctgcgcgg caaaagcgcc cagggcgaac aaaagcgaat cgccgggtag aatgggtgtg
     3541 accaccaggc cggtctcgca aaaaataacg agaaacatga tgacgtaggc ccaaatgccg
     3601 aaactctgga taatcagcgg caggtatttg tccagatgaa ggaaaaaatc tataaggtgg
     3661 aagagcacgt ccataagtaa tgtacgatga aaacctcaaa atataaataa aggtgacgtt
     3721 accgtataga gaagaacctc cggcctgtca acctgaaaaa gattttttct gttcaacaat
     3781 gccttcttcg gcgatgcatt tttaattgcc ggaattacac ggggctaagg tttgttcttt
     3841 ttgaaatttt gattgtaaaa gcagagtttg cccatgtctt ccgggtaggc aacaaaattt
     3901 tcatccagcg tgccgatagg ttcctgcctc aacatcattt ccagaaaagc atagaatttg
     3961 cgtttgaatt cgccaaagct tttgacctga agcgtgtgac gcaaaagaaa catcggtcgc
     4021 aaatgatacg tcacataagc cttctttgcc agacggtcaa tgtcgtcatt cgccagggcg
     4081 gtccagggtc ttgcaagaga cttatcctcg atattaccga gaatatactc ccgccagtaa
     4141 tcgacccccg tctgatcaac ggcttgccgc cagtagggcg tgagcggttt ggcggtgagt
     4201 ttggaaaatt gtacataatc gagcttcagg gatttggcga attttaccgt ttgttgaaac
     4261 gtctcccgtg tttcaccggg cgatccgacc aggaaaaatc ccagtgccct gatgcccaga
     4321 cttctcgtgg tctcgatggt gtcgcgaatc tgttgaaggc tgatcccttt gttcagacga
     4381 tccaggattt cctgcacacc cgactcgatg ccgaagtata tgcgggagca tcccgccgcc
     4441 ttcatcttcc gcagcagggc ttcatcaaca caatcaaccc gggtgcggca cgcccaggta
     4501 atatccatgc cattttgaat aatcccgtcg caaatggctt cggttcgttt gcggtcgatg
     4561 cagaaatcat aatcaaaaaa atcgatctca cgaatgccgt accgggtgcg gcattccttt
     4621 atctcgccca ggacggtctg gggactgcgc ggattgtacg acgtgtggcc ggcttcgcaa
     4681 aacaaacagc cgttgggaca gcctttgctc gtcaccatga cggtgaagtt tttgcgctct
     4741 gttgtaaatt cggcgtaaag gtcattgggc aaaagatggc gcgcaggatt cgggtagtcg
     4801 tcgaaaaccg gatccctgcc gtacggggtc atcgttgtcc gaccgttttc tttgtaaatc
     4861 agaccgggca catcggcgaa ccggcgccgg ccttccagct cctccagcaa ccgcggcagg
     4921 gtgtaaaaag cgctgttgaa gcagccgaaa tcgggtccct ctaccgacag ggactcgttc
     4981 gggtaaaccc tcagattata accgccgatg attaccggaa gcttaagatg ttttttgaga
     5041 aacgtcaccc attccaacgt ttcacggaac atataggtcg tcatcatgaa acccaggatg
     5101 tccggtttcc actgtttcaa ccggtccagg acgtcttccc tggtcaaacg cagcgtgcgg
     5161 gcgtcgatca gcagcgcgtc gtgtccggct ttctcggcga tcgcgcaaac ccaggccaga
     5221 gagagaggcg ggaaaagccc gaaatatttt tgaaccactc tcaggttttc ttcatgcagc
     5281 ttatattgaa atggattgaa aacgcacgcc agtctcattt tccctctaca cttttttcat
     5341 gaataacatg tactgtatca ttcaacaagg cattcccgac atattttctt tccgcgtgtc
     5401 ccgtggatta tttttgcatc atgcttccca gccaattatt caaaaacggt ctgataaaaa
     5461 acaggccgag aaaaaccgga agcagaccgt caacaaaagt catcagaagg cccgcggcca
     5521 gcaagtgacc cggagcggca taaccggcga aaagtacgat gaccgcagat tcccgcatcc
     5581 ccaaccccca gtaggtcatg ggcagatacg cggcaataat ggtcatggat ccccataata
     5641 aaagggcatc gacgggaagg tctatggaca gggatttgaa tgaaaacaaa ataatgagca
     5701 acttgcaacc ttctattgcc agagagtgga tcatgatcgc ttgacctgtt tgcgggtcat
     5761 aactttcaat gatttttctg acgttttccg gtaatctcag aattttatga atatataaaa
     5821 cgacggggcg aaaaaaattt tgccggattg atattgcaat cagtaaaacg atcagcagac
     5881 aggcagcggc aaagagcatt ttttgaagcg gaggatgaac ataaaataaa ccacccattg
     5941 agcagaaaaa aatcaacgcc gtgaaactga acatgtaacc cgataacagc gcaagaagtc
     6001 cccgggcgta agagagatga tgtctttctt tcatatagcc cagaatcgcg aattccccca
     6061 ttttaaacgg gagaatgcct ttgatggggt tgcagcccat tcggataatc acggcttcgc
     6121 caaatgaaat ccggcatccc gtcattttga caacacgttg aaatcttgcc gaggagaaga
     6181 ataaatgggc gagcacggaa caaaggaggc ccgcacagag gaatcgcata tccgtttgtt
     6241 gaagaaggga cacgacatca tggaacggaa ttcgggaaaa gagaacgaca aagatggcaa
     6301 gggtgaccag ccaggtaaag acaaatctca atatggcttt aggcgttgag agagttggat
     6361 attcccacac atgatgtttt tgaatgctca tattttctca ggtaaaagaa atatagaaag
     6421 gtgttgtcta tttgtcaatg gagaataatt caaaaaactg ctgaattctg aatcaacatg
     6481 tagtaaaagc tctgaacacc catcatgggt gctggcactg ttgcacgttc gcggcatgat
     6541 atgaaatttc ttttttgcgc aaatggggtt accgcttcac accatgctga aaaaaatgaa
     6601 tttaagctcc gacagacaaa gactgatcgt ttatgtcgtt ttaatagcgt tgtctctctt
     6661 tgttttctcg caggtcggcc aatttgattt tataacattt gacgatccgg tctacgtcgt
     6721 tgacaacccg tctattcaat cggggttcac aatggacggt gttatctggg ccttcggcac
     6781 aatccatgcc gaattctggc atccgctgac ctggctttcg ctcatgcttg attaccaact
     6841 cttcgggttg aacgccgggg ggtatcacat caccaacctg atgctgcaca tcctgagcac
     6901 gctcatgctg ttctggctct tgcatcgcat gacgggagcc gtttggagaa gcgcttttgt
     6961 tgccgctctg ttcgccattc atccgcttca tgtggaaacc gtcgtctgga tagcaaaacg
     7021 caaggatgtc ctgagcgctt ttttctggat gctgaccttg tgcctgtatg tttattacac
     7081 ggaaaagcca atggtcaaac gctatctatt cgttttcctg tcctttgcct gcgcgctgat
     7141 gagcaaaccc atggcggtta ccctgccggt gatcatgata gttttggatt actggccgct
     7201 gaaacggttt gcctcgaaaa aagaaaatcc cgttttctgg caggtcaagg aaaaattgcg
     7261 ttttttcatg ttatccgccg ttttttccct catcacaatc ttcgcccagt acgacaaata
     7321 cataaaacat gccgagacat cattcgattc ccgcctggcc aacgcgcctg tttcgctgac
     7381 gacctatctg gcaaaaaccc tctggcctta tgatctggcc gtcttctatc ctttttccta
     7441 tcagttgccc gcctggcaga ccgcaggatc aatagcgctg atcctggcca tcagcgttgc
     7501 cgtcatcctc gcggcaaaac gtctgccgta tttgcttgtc ggatggttat ggtatgtcct
     7561 tgctgttctg ccggtgcttg atatcttcag gtccagtgcg cgggccatgt ccgataatta
     7621 cacgtatctg cccgctgtag gcctttatgt gatggcggcc tggggtattc cggatctgtt
     7681 ttgccaaaaa caaatccgga gaaagatctt atggccggcc gggatcgccg ttgcggcaat
     7741 gctgtcggtt cttgcctggc aacaatgcag ttactggcaa aacagtttca agcttttcaa
     7801 tcatgctttg catgtcacaa aagataatta tctggcgcat aaccagagag gcaatgtgta
     7861 cagcaagtcg caacagtatc cgcctgccat tgcggatttc aacgaggcca tccgcataaa
     7921 accggactat gcttttgcct tcaacaacag ggggaatacg tatctccaac tcggccgcta
     7981 tcaacaggcc ataagcgact acagcgaggc catacgcctg aatcctgatt atgccagagc
     8041 ctattacaat agaggcttta cttacggtat ccatatgggc cggtaccaac gcgccattga
     8101 tgatttgaac gaagcgattc gaagagaacc tgacgcgccc gatatccatc tgacccgagg
     8161 catcgtttat tcgaatttag gcggccaaga actcgcatgc cttgattatc aaaaggcatg
     8221 cgacctgggc aactgcaaac ttctggatat ggcactgggc aaaggagaat gtctggcagc
     8281 ggatcttcag ccacacgaca gcggatacta tcacaacagc ggcatctctt atttcaagcg
     8341 tggacagtat cggatagcgt taggatactt tactcacgcc attcacttta aaaaggattt
     8401 cgctaatgcc tatcacaacc ggggaacgac ctacagcgaa ctcggccgct atcaacatgc
     8461 cgttgcagac tttaacgaat ccatccgcct taacccgggt tatcccgatg cttataaaaa
     8521 cagaggcatt gcttatttat tacagggcga gaaagaacag gggtgctctg attttaaaaa
     8581 ggcctgtgcg ctgggagact gtaaattact ggaacttgcc gaaagcaaag gggactgccg
     8641 ttgatgaatt ttattcatat ctcgaaataa aatcgtcaat ttaaaaaatg gttgagataa
     8701 ttttacattc gtcatacaca tttcaggaaa cttcatcgaa gtttgcgatg ggttgtcttg
     8761 gaagatgaac gatgaaacga cctaaaatac ttcttttcaa aagccacagc attgtttcaa
     8821 aagtcacagg cgtgacgccg ccgctgggat tactctatat cgccgccgct ctgcgggaac
     8881 ggctgggagc ggatgtgcgc gtgatcgtcg ctatgcttga gaacgacccg atgaacgtga
     8941 tggtatcgac gttgagacac tttcgtcctg atgtcgtggg aataagcgct ttgaccgcag
     9001 aggctttttt cgcgcacaaa gctgccgctg ccgtcaaggc ggaagcaccg tccgttccgg
     9061 tcatcatggg cggacctcat gcatcctgcg atccggaact tgttttgcag gattcctgcg
     9121 tggatgctgt tgtgatgggc gaaggggaag acacattcac tgaactggtc agtgtcacca
     9181 tgtctgaaaa atccgtctgg caaaatccgg acatattgca gaagattgac ggtctcgctt
     9241 ttcgcgttga cggaaagatg aagctttcaa aaccgcgcgc gccgataaac gatctcgacg
     9301 ctctgccttt tcccgcctgg gacctgatcg attacaaaaa atactggacc ctccccaaaa
     9361 tggcaacgga tggtgtgaga ccttatctga cgatgttcac gtcgcgcggt tgtccctata
     9421 attgtattta ttgccatcaa cttttcggca agtccttccg cagccggtca ccggaaagcg
     9481 tcgttgaaga aacagcacgg ctgatgagaa tgggcgcgga aagcatagag attctcgacg
     9541 atatatccaa ttttaacccg gaacgttttg acagaatgct ggaattaatg ctggaaaaaa
     9601 atctgcatcc cgtgttgagt ttccccaatg cattgcgcgc cgatttaatt cgtaaagaat
     9661 ccgccgacct gctgaaatgt gtcggcaccg gggagatttc cgtagcgatt gaaaccgcat
     9721 cggataggct acaaaaactg ctgggcaaaa atctctctct ggaaaaagcg aagcgcacca
     9781 ttgactggtt tgcggaccgg cgcattttca ctcgcggttt cttcatgctc ggttttccaa
     9841 cggaaacgca agatgaaatg cttgccacaa tccgttttgc tcatgcttcc cgattgcacc
     9901 tggcactgtt ttttacgccc aatccttttc gcaatacagc gctctatgat cttttcgtcg
     9961 atacgggcaa actcccttca acgtccacaa gcattgacta tgaatattac ggagcgcctt
    10021 tcaacggaag cgatgtttca gaccggcgct atcggatgct ttaccgctgg gcgtattacg
    10081 gtttctattt caatccggtg cgcctctggc gtatagcgca agacaggccg acccttgccg
    10141 atattcctgc aagcgcgtgg acgcgcctga ataacgtggt atctttccgt cgactcaaag
    10201 aagaagatac gtccctggtg taagcagtac gatgagagat tttattcatc gtatataaat
    10261 tgaatctaat gtttaataat tttgtccttc gaaaataagt aatattatga attctttgtt
    10321 cgatttatca aacctgtatg gattagcatg caacatctgg ccgcgttttc cgtatgacat
    10381 cccgcagctt caaactgcat taaaaccatt cctctatctt tttaatatca ccaatcaatg
    10441 caatttaaac tgttactact gctgtcagaa gacgagcctg acccggaatc ggcccgaact
    10501 atcctttgaa gacatagccg gcattattaa aaaattaccg cgatatgcgg ttatcgtgtt
    10561 ctccggagga gaacctcttt gccgggacga tttcactcag ctattgcaag aaacccgccg
    10621 tctaaagaga aaaacagcgc ttttaacaaa tggactgctg ttgaacgacg atatcatttg
    10681 cgaaatgatt caaaacagat tgctgaatat cggcatcgcg attgacggtg ataaatacta
    10741 ttatgaaaaa ataaaggggc ggggcaatta tgctgttgtg atggataatg tagaccgtct
    10801 ggcctatcac aagaaaaaag ccaaaagttc gtttccggca tttgactgga aagtaacggt
    10861 ttttcccgaa aatgtcagcc gcctcccgct tctttaccag caggctgccg atcatggcgc
    10921 tgattcattc acagtgtcct tccccaaaaa aaatgatttt cagttcaacg actgtctgca
    10981 cgacatcgca gtgcttgatt gcgaacccgt atcaaacggg aactaccgct tcccggaaaa
    11041 catcgaagac atttataagg aattattctc catagccaga acttcccgga ccagactcag
    11101 aacatatccg cagatgaaac aggcggaaga tctgaaaaac tattttgaaa cgtctgcaat
    11161 ccaaaaaaga tattccgctt gccgtatgcc gtggtcagga atggtcataa gttccaccgg
    11221 agaagcctac ccctgcttgt ccgtcagaat aggggattta aagaaacaat cgcttgccca
    11281 ggttcttacg tcgccggaaa atattcggtt tagaaaccaa ttaaaaaaaa gaagtctctt
    11341 tgcaatctgc gacggttgct gttatgcaag gttaaaaccg gttttgaaaa gagagcgagc
    11401 tgtcggtgcg taaaatactt tttatctacc ctcgattagg atacatggat gcccaacgca
    11461 gcaaacctgc accgccgctg ggaatcctcg ctgccgtgtc gtttttgaaa gatacctacg
    11521 agataatcat tctggatcag cgtctttttg cttccgacga ctctttttac gcaaaacttt
    11581 acaatgtgct gcaaaatcag cctctctatg ccggactgtc cgtttacagc ggcgggatga
    11641 ttaaatttgc tctggaaatc agccggtata ttaaagagca atcatccgtc cccgttgtct
    11701 ggggaggcat ccacccgtct ttattaccgg aacaaacact ggaaaatcag tatatagatt
    11761 atgtcatcca gggagaagga gaactcaccc ttcccgaatt ttccgacagg cttgtccaat
    11821 acggccagaa tatgccgccc gtcagaggcg tctggatcaa aaagcaggga aagattgctt
    11881 acggaggaga cagagattta atagatttac aggccgcccc tgtcattcca tacgatctgg
    11941 tggatttcaa caattacgtg caatattaca acggtcacaa atacgcttat taccaggcat
    12001 cgcgggggtg tccgcgacat tgccggtact gctataatca tgttttcaac aaagggaaat
    12061 tccgtgcaaa atctgcggat aaaatcattt cagaaatcaa aactctggct gagagatacg
    12121 cttctttcac gggaatcttt tttgttgacg acaacatatt cgcaatgggc aaggactatg
    12181 tgatgaatct cggaaagggg ctgggcgagc ttggcctgcg ctgggcggtt cagggaagcg
    12241 atatcattgc gctgaaagac tacaccgaaa gcgacttcaa acgtcttgaa acgtgcggtt
    12301 tgacaagact caccattggg gttgaatcgg cgtcgaagaa gatgcgaacg ttaatcggca
    12361 aacagggaag cccggccgat atcgaacagg ttatggccag attgagcggg acaaaaattc
    12421 tgatctggtg cagctatatt atcagtttgc ccggagaaac cctcgatgat ctccgcgcat
    12481 caatcaaact gattttccgg ttgcagcaca tcaacccaaa tgtccgcaac agccccttct
    12541 atatgtacat ccctttttcc ggaacaccgc tgtatgaaca gtacaaggat gttttccccg
    12601 gtcccgaatc actggaagaa tggggtcagg tgggatggga aagagaacac acaaacagct
    12661 tcgccgatta tctaaaagat acgcattttt ttcagtcgct gtttttaacc agcctgctcg
    12721 atgacgataa agtttctgac ttttccaaaa acaaactttt ggtttttctg gcaaagtgtt
    12781 atcgtcctgt tgcccgctgg cgtttaaaga acctgttttt caaattcaat atagaattat
    12841 cgatgttcaa aaaatttttt cctgatatct tctgatatga attgcctggc ttgatcatcc
    12901 ccgacgctcc tccattcgct caagactaaa agcccgccaa acatcatccg gcaaaccgaa
    12961 cgtaataaat ggaagctttt cacagcgcac gctggaaagt cttcgatcca taggaaatga
    13021 cgtacttacc taatgtcgct cgccgacccc gccgcaggta tcgttggata cgctcgctgc
    13081 cggattcaac agcgagttta tcattgacag gcaaacatgt ttttcctata ccgtctcata
    13141 tggccggaaa atctatatca caactccaga acgcatctgg ttttctgata caatttttaa
    13201 gaagaaaatc agatattgcc gccctcattc tgatcgcggc ggtcatcgca ctgccgtcga
    13261 tctcaccgct ttcatcgggg agtaaagtta actttaatga tgatttcttt tattatgccg
    13321 gaatgcacga atcggtcaga caagctgtcc tcgaacatca tacttttccg actcgctcgc
    13381 tctggatagg aggcggttat ccttctctcg gcaacccgga agaccctgca ctaaacccgt
    13441 tgactttgat caccattgtt tttggatctg ttatggctct aaagatcatt acttttatgg
    13501 cgttgctcgt cggggggttg agcagttatt tactggcaag acatattctc ggctacacca
    13561 agtggggatc gcttttctgc ggactggttt ttggtttaag cttgtttgtc cccctccggg
    13621 tatacgacgg caatactaac gaggtttacg tcgcttttct cccgttatgc atgctgctta
    13681 tcggactggc atgccgcgga agaaagacag ccgtcttcat cctggctttt gttttttata
    13741 ccatgctttc ggacggcaag cttatcgctt tgatgatttt tctttatctc ggaattctat
    13801 gtttgctgga tatcattccc tcatttaaca tttttgcgac aaaaaattta aataagatga
    13861 acattaaacc tttaaaagtg cttttcctgg cgctgacgat aacctttttt gtcggtatgc
    13921 tgcgtatttt acccgtattg gatatgatag aaacaatggg agggttacaa agcaactttc
    13981 tttggttcaa gcccatcact tacagcccca gcggggttgt tacgttgcag gagttatggc
    14041 ataatttaat cggctataaa gatattctta acatcgcaac gatcggatta atccccgtgc
    14101 ttcttgcttt aagcgttttg tttatatttc ccccaaaggc atttccctgg gccgttacca
    14161 tttttttatt tacatggttg atgctggcct acaatgctcc cgtggacctg tcaaagctgc
    14221 tttggcacct ccccgtgttt aatataatca ctaaacataa gtattacgct tttccaatag
    14281 tcttctcttt gattattgtc gccggacaat tcttctggtt attgtcaaaa atacgttcaa
    14341 agtggctgga acatgcattg gctgccgcat taatcctttt gagcgtatgg tttttgtatc
    14401 cgcggttcga tgccattcag aaaaacacat atacttacga tatcccggaa gaatttctcg
    14461 tcaggcaaaa tgagttttat aatatacagg gcctgggaat tccacgcttt cgggcaaaac
    14521 cgctgaattc cgttatatat acaaacgtca ttcgtaatgt cggcatcatt gacagcgtca
    14581 cacattatgg cgaaaaagcc gtcccaaaat acttcgttga tgcggaaggc acacttatcc
    14641 ccaatcccgg atacaaagga gaagcccact ttgccgaccc cgcaaacaca gccaaagcca
    14701 cttttcggcc aaattccatc atggttcagg tgaacctgcg aaaaccggac atgcttgtga
    14761 tcaatcaaaa ctatgaaagg aattggcatt cggagcacgg aaggattttc aataagggtg
    14821 gtttgattgc attgcgtctg gacaaacccg gcgtttacga cattaccctt cgttacttct
    14881 ttcgcagttt cttcgtcggt ttgttcttaa gtattgtcag cctaatagca ttgatttcta
    14941 tctgttggag ctacaaaacc ggaagattaa tcaagtggtc acatgatgcc cccatccata
    15001 taagatggat gcctcgattc atcctttggc tcataaatta agcattacta aaaaactact
    15061 gaattctgat tgaacatgta gtacaagtac ggcaaaaata atccgatata ttaaatacga
    15121 tggataccgg tcttcagaga caaaaggttt ttatatatat ttttttgacg gttgtctcac
    15181 tggctgttta tggtcaggtg ggacaatttg acttcatcaa ttttgatgat aatgtttata
    15241 ttaccgacaa ccatcatgtc cagtccggca tcactctggc gagtatccgc tgggcattga
    15301 cgactaccta cgcggaattc tggcatcctt tgacctggct gtccctgatg gccgattatg
    15361 agctttacgg tcttcatgcc ggaggatacc atgtcaccaa cctcatcttt catatcctca
    15421 gcaccttgtt gttgttctgg ctctttcacc ggatgacggg agccctctgg aagagcgcct
    15481 ttgtcgccgc tttctttgct ctgcatcccc tgcatgtgga atccgttgcc tgggtttccg
    15541 aacgcaaaga tgtcttaagc gctttcttct ggatgctcac cttatgcctg tatgtctatt
    15601 atgcggagaa acctgttatt aaaagatatg ccc
//
LOCUS       DAONQZ010000052        15510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4234_length_15510_cov_17.057069, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000052 DAONQZ010000000
VERSION     DAONQZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4234_length_15510_cov_17.057069"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLR13_08530"
     CDS             complement(<1..352)
                     /locus_tag="PLR13_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQC19423.1"
                     /translation="MKILLVNPVTSNISLSSPDLGLGYLAGALKRENHQVHILDCVNL
                     KLSLEQFEHYIDACDVDVIGFKVFSTDVFSVKRSLQCVKKKRPDMKIILGGAHPSTFP
                     EQTLQYFEEADFAIR"
     gene            complement(374..1846)
                     /locus_tag="PLR13_08535"
     CDS             complement(374..1846)
                     /locus_tag="PLR13_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HQC19424.1"
                     /translation="MKALLIYPLIPDTFWNFRHVLKFIRKKAAHVPLGLLTVAAMMPK
                     DWEIKLVDMNAQHLSDATIQWADMVFIGAMIIQKESVQQIISRCRKMERVVVGGGPLF
                     TSFPEVFEDVDHLILNEAEITLPPFLKDLQSGNLNRIYQNEERPNITMTPLPRWDLID
                     INTYASMAVQYSRGCPYNCEFCDIVYLNGREPRVKSNEQMLKEFDSLYNIGWRGSLFI
                     VDDNFIGNRLQVKTLLRKLIPWQAARNFPFTLYTQATVNLALDEELMQLMTAAGFDSI
                     FLGIETPEKDCLLECGKYQNLQIDIVEAVKTIQRHGMEVTGGFIIGFDSDPPDIFERQ
                     IKFLQDSGIVRAMIGLLHAPPGSRLYKRLKKEGRLLEITSGDNCDGSMNFIPKMGYDF
                     IRKEYHHLLNKIYSPRQYYLRVLEFLNTYVPARRHPVTVLGIVAFLNIILHLGILDNW
                     RNSVYFWKLMIKTLIFHRRSMREAVTLMMFGYHFRKLLKK"
     gene            complement(1863..3368)
                     /locus_tag="PLR13_08540"
     CDS             complement(1863..3368)
                     /locus_tag="PLR13_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19425.1"
                     /translation="MKIAFFNIAYENLAVSILSAIAKRAGHTVKLMHTPTLFKDGLTR
                     EFKRLARIFSNEKLIFKQLTAYQPDLIAFSVVTFDYQRAMHYAEICRKLCPKAKIVFG
                     GIHVSCVPEVVIENDFVDYAVIGEGEEAFPEIIKHIEAGCPPEPIVNTWYKSKDNTLI
                     KGKQVGFIQDLDSLPHYDKEIWKDIFPLKTYYLTMASRGCPYNCSYCFNHFFRNIPEE
                     KSNYVRRRSVKHVIEELSIAKTKYNIHTIEFWDDIFIFDKKWLKEFLETYKKEIDLPF
                     KCYIHVNLFDEEICIWLKEAGCRWVDFGIQHINEKYRKKYLKRHETNANIENTLRLLK
                     KHKIVSFADYIVGLPGDTIEHNEEARLFFLKNMPDIIEPYWMSYHPKTDIIQKGFEHH
                     VLNDETMNAIHRGLKHSYFESAPASKDYHDDYYFIFKVLPALPNFMRTRLTYDVARKI
                     PYFIKLPFFIYSIFYLAIKHNSPRIKYLTSFYIKQTVWMIKSSLHLKKASL"
     gene            complement(3543..4286)
                     /locus_tag="PLR13_08545"
     CDS             complement(3543..4286)
                     /locus_tag="PLR13_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19426.1"
                     /translation="MANNNKQEKVIPEISVVILCFQEGRRLSAFVNRTIEILESLPVS
                     WEIVLVANWDNENDDTPGEAKKIAAQKNHIKVVSEFKKGGMGWDARKGFEQASGRFIC
                     LIDGDGQMPPESIIGVYHKIIYDHCDLVMTYRTQRGDSLLRKINSYTYNFAFHILFPA
                     VNVRDANSKPKIFTRNTYSKMQLNADDWFLDAEILIQCTRLKLKIAEIPVTFDKCLSR
                     KSFVKIDAIWEFIKNLLSARIKEYFIKNN"
     gene            complement(4276..5874)
                     /locus_tag="PLR13_08550"
     CDS             complement(4276..5874)
                     /locus_tag="PLR13_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19427.1"
                     /translation="MTKKTYIWLWSAFLACLILLPYLQMHSHDFIIWDDSFYIYDNPM
                     VTQGLNLLGVGWAFITFATANWHPLTWISHMLDSSIFGPTPFAAHLMNSLWYAGCILL
                     SFFLFLQLGASFPAAFLMAAFFGLHPLHVESVAWASERKDLLCAFFFLSAMLAYLHYA
                     RKRILSLYLLTTGFFILALLSKPMAVTWPCVVLLLDYWPLDRWKNDAKRVVLEKIPWV
                     VLASLSSIITVIAQHQRAAVKSLVEFSLTDRLANAGISYLIYVRQTVWPSGLTVFYPY
                     PLHIHLGDAFLACFFLIIVTIVVVRQKQQYPYLLWGWLFYLGVLFPVIGIVQVGSQAH
                     ADRYMFLPQLGLILAVGLFLDKTVVKMKLRRGLAVLMMIIAAVLMIITFRQVSYWKNS
                     KSLFYRNLEVAGENELAHFNLGSAYLENNELDMAVIHLTATAQMNPQDATTFNNLGIA
                     YARKKIDALAEACFHRAILLDPRTVQPYVNLAVLKTNQGRFEEALDHINTAVQLAPDY
                     KEARDIRNALEKAVLRHKNNADGQ"
     gene            6145..8121
                     /locus_tag="PLR13_08555"
     CDS             6145..8121
                     /locus_tag="PLR13_08555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19428.1"
                     /translation="MIMRSSIDFVKKPLLAAALVAAIILVSGVLRILFYVKSPDVIFL
                     VNRAGAQWIKYDDEFELQAKPSGRIQCHFKYVFHTNETIREARISVQALKKAEVSFDG
                     VKIYSANDQFSRWKQVHDIKIPLDIAPGGHEIVISATSENSYAAILAYSKELQVRTGT
                     GWFASYDGKNWRAAVPVTTFTPPAVIMKFPTSFEALRAVWSYLSVVFIMVFCFSWIYG
                     SGKKVFPKLLQWIVQPCHVRWGLLGLWAILSINNMFKLNYQTGSDVWGHIDYIDYIAT
                     KGTLPLAPEGWQMFQAPLNYLLSAPLYAVLSKWLDLPSVVKIMTIIPVLCGLLQIEIV
                     YRTARLVFTQRKDLQIIAVIAGSLLPVHTYVCQYVGNEPLTACLVSVLILLSVELILP
                     DRKEHGNGYFVVMGVVWGLALLSKMTALLLAPVVLLVLIFHVWSLQGKLKNPLRPVLI
                     VFLVPVIVAGWYYLRNYLKLGNAFMGLFDPLQMMQWWQDPGYRIWPHLLSFGEALYRP
                     FCAGLTSFWDMFYSTLWLDGINSGLIDFIPWNENFMIAGSLLALLPSVFILTGLVNLA
                     LNKKTEYRNAVILSAASILIFLMAMIDMYIACPVYSRTKAGYTLGLLPCYAILIAAGA
                     EPFLRHRIIRSFALAFFACWAFAAYAAYFVVNAP"
     gene            complement(8142..9266)
                     /locus_tag="PLR13_08560"
     CDS             complement(8142..9266)
                     /locus_tag="PLR13_08560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQC19429.1"
                     /translation="MSKILLKILTNMLLLVSVFILPSFYPAGPAVYFRLFLLTVILIL
                     SVNLFKVLFIDRNIPKIIANTATVCMAVFVTFIVLEAAFMFIPRSHSADYTLASKLWY
                     AKYWKPINSMGFRDKEPAKSNHVILFVGDSFTAGHGLKSVDERFSDIVAKKLNKKKND
                     FTVMNIAKPNLDSREEYDTMMHVLYLNRIKPEKIILQYCGNDIEGAAARHGLIFEGFK
                     PPSDMNRFLLLVGSGSYFFNYVYFLFPREYLGIPYLTYLDTAYKNNKVLSDHKKDLML
                     FIDYARKNSIQIIAVVFPFLADLAMSDAMYVNDITGFLESNGTNVINVSSLTANMPIP
                     ERIVNKNDGHASAKVNRMVAVEILKIMDGQNESHSSLSER"
     gene            complement(9527..11140)
                     /locus_tag="PLR13_08565"
     CDS             complement(9527..11140)
                     /locus_tag="PLR13_08565"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HQC19430.1"
                     /translation="MKLSIKIIPVFIILFTAIVYSGCSSLDDAPPTNYTVAFNSQFND
                     PESTTVMPNPTSITVEEGQSVGTLPTAPSMSGYKFGGWWTGKGGTGTLFTAATAVNSD
                     MIVYAFWYKYQVIFMSDGAVYASRGVTPPSKTLSTLPASPTKTGYTFAGWYTAPDGGG
                     SQFLAGTEVTADITVYARWATGTVHIVTYNSDGGTAAGTQYVVAPATNVGTLPADPTK
                     VFNTFDGWYTAVGGGGTAFTGSTPVTENITVYAKWVSDSGYTVEYNSYGGASINDQYV
                     ILSKGNTVATGTEEGILPEPAKRCYKFAGWYTESDGEGSAFTADTVLNDSNTVEGKIT
                     LYAKWDWDYPSADYPLITSPFEVGDYGPSCVGKVFYVTDGGLHGLEAAPPNWYQGSEE
                     ASSVAWINGDPQVDENDDIYQKTQSTTNGNTSEAIGTGLANSNAIVAQVEEAGGTDIP
                     YAAALCLDYSVGAGDYLFNDWFLPSRDELAQLFTNRDVKRSGGFLDEGYWSSSEYSQW
                     DAWSQYFSNGAQTGTYKSQGRLVRPIRKF"
     gene            complement(11405..12379)
                     /locus_tag="PLR13_08570"
     CDS             complement(11405..12379)
                     /locus_tag="PLR13_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HQC19431.1"
                     /translation="MTGVKSNQSHNETVLRGQASVPDERFDKLVEIYKPAKISPAKVP
                     FVDVHAVGEHPWEAIRQNLSGTDGILHVVDGFSLPDIQLSIEAYHKLEDELVLSDLLI
                     IEKKMEKLAKMPRKAIPAQEAAQNELLPRLKDCLEAGKPLRAAGLTPTEIFTLKSFSF
                     WTIKPELVVVNTAEDNLSFAEQFAAAARLKEPVMGICCKIEAELAGLDAADQKEFLAS
                     MDIAEPAFGRIIRAAFSLLGRISFFTVGEDEVKAWVIPAETKAPKAAGAIHNDFERGF
                     IKAEVMHYDDFVAHGFSAAQVKSAGRLRLEGKEYIVQDGDIITFRFNV"
     gene            complement(12602..>15510)
                     /locus_tag="PLR13_08575"
     CDS             complement(12602..>15510)
                     /locus_tag="PLR13_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQC19432.1"
                     /translation="AIGLLIFLVVLGIAGFFIAPPLMKPYIMKKMSEALHRKASIEKI
                     SINPYAVSITLKKFSLEDPGKPKAFVAFDELYVNADLMSSLFRRALILKQITLTNPYV
                     GISRKPEGSYNFSDLLPKDEAKPDKEDKPFLFSLNNIQIINGNIDFEDAPKKTNHTVR
                     ELNVSVPFISNIEYHLEKYVEPKFSTNINNNKFELAGKTQPFLTSRATSFDINIQDID
                     IPYYLNYVPAKMNCQLKSARLDTNIKLNFIVNEDKSPALTLNGNVVLRDVILDDLKNN
                     KILHLPSLNVNMTSVEPFVSNIHLARIAIESPELVIRRDKEGEINLLNLTKKQVEKLP
                     QKEAAAASKKKSGFKFRVDDVLLYKADIIYMDSQPSDPVKLHFSPLSFKAANLSTEKG
                     ATGKVELEGILNKKGEFKDRVTLTLEPLSVDVQSDWKNIGIRAFQPYFTDKVKMDITR
                     GTISSTGNFSLSRNKKDELVIKCTGSVSVSNLAALDKAQSNDFLKFKRLSLDKIKFGY
                     NPFSLSINTISLADFYTRIIINPDASLNVQDIFGDNQASKETAIKKEEKKPVTAETKK
                     KDNQETADIKIGKVVFKNGHIDFSDNNIQPNYSANMFNLTGSVTGLSSKEFSRADVAL
                     KGNLGYGSPIEIAGKINPLAKDLFVDLKVGFKDIELSPITPYSNKFLGYPITKGKLTF
                     NVSYLIDKRKLDSQNKVLIDQLTFGEKVESPQAVKAPVTLAATLLTDRNGQINLDLPV
                     SGSLDDPKFKVWSVIWQIIVNLITKAVTSPFALLSSLTGGGEELSFIEFDYGSAVISA
                     EETKKISMIGKALNDRPNIKLDIESYVDAEQDKYALKKAELDRRIKAQKMKETMSKDN
                     QQIAVENVVLTEQEYDKYLKQVYRSADFSKPRNILGMQKTLPPAEMEKLMLANIDVTD
                     SDLRQLAARRAQNVKELMLQSDIAADRIFIVESKSLTPEKKEKVKNSRADFKLK"
BASE COUNT         3957 a         3453 c         3614 g         4486 t
ORIGIN      
        1 ctctgatggc aaaatcggct tcttcgaaat attgcagcgt ctgttcggga aacgttgaag
       61 gatgcgctcc gccgagaatg attttcatgt ccggacgttt tttcttcacg cattgcagag
      121 atcgcttgac cgaaaagaca tccgtggaaa agaccttgaa accgatcaca tcaacatcgc
      181 aagcatcgat ataatgctcg aattgttcga gagaaagctt gagattgacg caatccagga
      241 tgtgaacctg atggttttcc cttttaagcg ctccggccag atatcccagg cccagatcgg
      301 gagaggacag ggaaatattg ctggtgacgg gatttaccag taaaattttc ataaatttgt
      361 ctttcccggc cgattatttt tttaacagtt tacggaagtg atatccgaac atcatgagag
      421 tcacggcctc tctcatggac cgacgatgaa atattaatgt tttaatcatt aacttccaga
      481 agtaaacact gtttctccag ttatctaaaa tgcccagatg gagaataata tttaaaaaag
      541 cgacgattcc caaaaccgtt acgggatgac ggcgtgccgg aacgtatgta tttaaaaact
      601 ccagaacgcg caaatagtat tgtctcggtg aataaatttt gttcagaaga tgatgatatt
      661 ctttccggat gaaatcgtaa cccatcttgg gaatgaaatt catggatcca tcacaattat
      721 cccctgatgt aatttccagc aatcgtccct ctttcttaag tcttttgtat aaacgggagc
      781 cgggaggtgc atgcagcagt cctatcatgg ctcttacaat gccgctatct tggagaaatt
      841 ttatttgtcg ttcaaagata tcgggcggat cgctgtcaaa accaataatg aaaccgccgg
      901 tgacctccat gccgtgtcgt tgaatagttt taacggcctc tactatgtca atttgaaggt
      961 tctgatattt cccgcattcc agaagacaat ctttctccgg cgtttcaatt cctagaaaga
     1021 tggaatcaaa tcctgccgcc gtcattaatt gcatgagttc ctcatcaaga gccagattaa
     1081 ccgttgcctg tgtgtagagt gtaaaaggga aatttctggc cgcctgccag ggtatcagtt
     1141 ttcgcagaag cgttttgacc tgcaaacggt ttccaatgaa gttatcatcg acgatgaaaa
     1201 ggcttcctct ccagccgatg ttatacagtg aatcaaattc tttgagcatc tgttcattcg
     1261 acttgaccct gggttccctt ccgttgagat agacgatatc acaaaattca caattataag
     1321 ggcatcccct tgaatattgc actgccatgg aggcataggt gttgatatct atgagatccc
     1381 agcgggggag aggggtcatg gtaatatttg gtctttcttc attttgataa attcgattca
     1441 aattgcccga ttgaagatct ttgaggaaag gaggtagggt gatttccgct tcattcagaa
     1501 tgagatgatc aacatcttca aacacttcgg gaaaactcgt aaaaagaggc cctccgccaa
     1561 cgaccacccg ttccatttta cgacatctgc tgataatttg ttgcacggat tctttttgaa
     1621 taatcattgc accgataaaa accatatccg cccattgtat cgttgcgtcg gatagatgtt
     1681 gtgcattcat atccaccagt ttaatttccc agtctttggg catcattgcc gcgaccgtca
     1741 ggagtcccag cggcacatgc gccgcttttt tccggatgaa ctttaaaaca tgccggaaat
     1801 tccagaaggt atcgggaatc agaggataaa taagtaaagc tttcacggga ctttttcctg
     1861 attcataagg aagctttttt tagatgaaga cttgatttaa tcatccacac cgtctgtttt
     1921 atgtaaaagg atgtcagata ttttatgcga ggactgttgt gcttaatggc cagataaaaa
     1981 atggagtaaa taaaaaaagg caattttata aagtacggta ttttccgggc gacatcatac
     2041 gtaagtcttg tcctcataaa gtttggcaga gcgggaagta ctttgaaaat aaaataataa
     2101 tcgtcgtgat agtctttcga tgccggtgca ctctcaaaat agctgtgctt cagcccgcga
     2161 tgaattgcat tcattgtttc atcattgaga acatggtgtt caaatccttt ttgaataata
     2221 tccgttttgg gatggtaaga catccagtaa ggttcgataa tatcgggcat gtttttcaga
     2281 aagaaaagtc tggcctcctc attatgttct atggtgtctc cgggcaggcc gactatataa
     2341 tcggcaaaag aaacgatttt atgttttttc aaaagcctca atgtattttc gatgtttgcg
     2401 ttggtttcgt gacgtttgag atatttcttc cggtattttt cattaatgtg ttgaatgccg
     2461 aaatccaccc accggcagcc ggcttctttg agccatatac agatttcctc gtcaaataaa
     2521 ttgacgtgga tatagcactt gaagggaagg tcaatttctt ttttgtatgt ttccagaaat
     2581 tctttcagcc attttttgtc gaatatgaaa atgtcatccc agaattcaat ggtgtgaata
     2641 ttgtattttg ttttggcgat ggaaagctct tcaatgacgt gcttcacgct ccgtcttctg
     2701 acataatttg atttttcttc cgggatattt ctgaaaaaat gattaaagca gtaagagcaa
     2761 ttgtatggac atcctctgga ggccatggtg agatagtatg ttttcaacgg gaagatgtct
     2821 ttccagattt ccttgtcgta atggggcagg gaatcgagat cctggataaa accgacctgt
     2881 ttccccttga tcagggtgtt gtctttggat ttgtaccacg tgttgacgat cggctccgga
     2941 gggcatcctg cttcaatgtg tttgataatt tcgggaaaag cctcttctcc ttcgccgata
     3001 accgcataat ctacaaagtc attttcaatt acgacttcgg ggacgcagga aacatggatg
     3061 ccgccaaaaa caattttagc tttcgggcaa agctttctgc aaatttcggc ataatgcatc
     3121 gcccgttgat aatcaaatgt gactacggaa aaagcaatga gatcaggctg atacgctgta
     3181 agctgcttga atattagctt ttcattactg aagattctgg ccaggcgttt gaattctctg
     3241 gtcagcccgt ctttaaaaag agtcggcgta tgcattaatt ttacagtgtg ccccgctctt
     3301 tttgcaatag ctgaaaggat cgataccgca aggttttcat atgctatgtt gaaaaacgct
     3361 attttcatat aatttttttg tgagaattaa agagtctttt gctttttttg aaaatcaggc
     3421 aatgcttttt aattcattta tggattatgg attaaagcaa tacgtttaag tttttaagat
     3481 ataagtaata taaaatttcg tgtcaaggaa atgttgcgct gaaaagagca atgtctattt
     3541 ttttagttat tcttgataaa atattccttt attcttgcgg ataaaaggtt cttgataaat
     3601 tcccaaatgg catcgatctt cacgaaagat ttccggctga ggcatttatc aaaggtcacg
     3661 ggaatctctg cgattttgag tttcaatctt gtgcattgaa ttaatatttc cgcatccaga
     3721 aaccagtcgt cggcattaag ctgcattttc gagtaggtgt tccgggtgaa aatcttcggt
     3781 ttggagttgg catccctgac gttgacggcc ggaaatagaa tgtggaaggc gaaattatag
     3841 gtatacgaat tgatttttct caacaagctg tctccccgct gcgtccggta ggtcatgacc
     3901 agatcgcaat gatcatatat tattttgtga tagactccga tgatgctttc cggcggcatt
     3961 tgaccatctc cgtcgataag acagataaat cttccggagg cctgctcaaa gcctttccgg
     4021 gcatcccagc ccatgccgcc tttcttgaat tcgctgacaa ctttgatatg atttttttgc
     4081 gcagctattt ttttggcttc tccgggggta tcgtcgtttt cgttgtccca gttggcgacc
     4141 aggacaattt cccaggatac cggcaggctc tccagtatct cgatcgttcg attgacaaac
     4201 gcacttaatc ttcgaccttc ctgaaagcac aggatgacga ccgatatctc cggaattacc
     4261 ttttcttgtt tattgttatt ggccatcggc gttgttttta tgacggagta ccgccttttc
     4321 taatgcgttt cgtatgtccc ttgcctcttt ataatccggc gccagttgta cggctgtgtt
     4381 gatgtggtca agcgcttctt caaagcgtcc ctgatttgtt ttcagaacgg caagattgac
     4441 atacggttgc accgtccggg gatcgagtaa aatggcccgg tgaaaacatg cttcggcaag
     4501 cgcgtctatt ttcttccgtg cgtaagcgat gcccaggttg ttgaaggttg tggcatcttg
     4561 agggttcatt tgcgctgtgg ccgttaaatg gatgacggcc atgtccaatt cgttgttttc
     4621 cagataggcc gagcccagat tgaaatgggc cagttcattt tcgccggcaa cctccagatt
     4681 ccggtagaat aaagatttgc tgttcttcca gtaggacacc tgcctgaatg taataatcat
     4741 gagaaccgcg gcaatgatca tcatcagaac ggccaatccg cggcgcagtt tcatttttac
     4801 aacggttttg tccagaaaaa gtccgaccgc cagaatcagt cccagttggg gtaaaaacat
     4861 ataccggtcg gcatgggcct gactgcccac ctgaacaatg ccgattacgg gaaacaaaac
     4921 gcccagatag aataaccagc cccataaaag ataggggtat tgttgcttct gacggacgac
     4981 gacgatggtt acgatgatca gaaagaagca ggccagaaag gcatcgccga gatgaatgtg
     5041 aagaggatag ggataaaata cggtcagtcc ggaaggccag accgtctggc gaacataaat
     5101 cagataagag atccccgcgt tggccagccg gtccgttaag gaaaattcaa caagcgattt
     5161 gacggcggca cgttgatgct gggcgatgac cgtgataatg gatgaaaggc tggccagcac
     5221 gacccaggga attttttcaa gcacgacccg cttcgcatcg tttttccagc ggtccagggg
     5281 ccaatagtcc agaagaagaa caacgcaggg ccacgtgacg gccatgggtt tggacagcag
     5341 cgccagaatg aagaatcccg ttgtcagcag gtacagagat aaaattcttt tgcgcgcgta
     5401 atgcagataa gcgagcatgg cggacagaaa gaaaaatgca cacagcaaat ccttccgttc
     5461 ggaagcccag gcaacggatt ccacatgcag cggatgcagt ccgaaaaagg ccgccatcaa
     5521 aaaagccgcc gggaaagagg cgccgagctg caaaaataaa aagaaggaca agagaataca
     5581 gcctgcatac cagaggctgt tcatcagatg agcggcaaac ggcgttggac cgaaaatact
     5641 ggaatccagc atatggctga tccaggtcaa cggatgccag tttgccgttg caaatgtgat
     5701 gaaagcccaa ccgacgccga gcaaatttag gccttgcgtg accatcggat tgtcgtaaat
     5761 gtaaaatgaa tcatcccaaa tgataaaatc atgcgaatgc atctgcaggt agggtaacaa
     5821 tatcaaacat gccagaaacg ccgaccatag ccatatgtat gtttttttcg tcattccgtt
     5881 ttccaatgat atgcctggcc acactttatg tcaattgatc tgtatgatgc gaatttatat
     5941 gaatctgttg cggggtgtca acataaaaca atcggcacaa tccgtttgat gttcaaacgg
     6001 tcgtgaaccc gcatcattag tttctgattg acacgtcaac gggatatccc atataacttt
     6061 tccaacgatt aaaaccccgt tattttgact tgtatacaat atacaggata acttagattt
     6121 ttcggaaaga cggttttttt acaaatgatc atgcgttcat ccatcgattt cgttaaaaaa
     6181 cctttactgg ccgcggctct ggttgccgcg atcattcttg tttccggtgt cttacgaatc
     6241 ctgttttatg taaaatctcc cgatgtcatt tttcttgtca atcgtgccgg agctcagtgg
     6301 ataaaatacg acgatgaatt cgaactgcag gccaaaccgt cgggccggat acaatgtcat
     6361 ttcaaatatg tttttcatac gaatgaaaca atccgggagg cccggatctc tgttcaggcg
     6421 ctgaaaaaag cagaggtctc ctttgacggc gtaaaaattt attccgcgaa tgatcaattc
     6481 agtcgatgga agcaggttca tgatatcaaa atacccttgg acattgcacc gggaggccat
     6541 gaaatcgtta tttccgcaac cagtgaaaat tcttatgccg ccattttggc gtacagtaaa
     6601 gaactccagg tccggaccgg aacgggatgg tttgcgtctt acgacggtaa gaactggcgt
     6661 gcggctgtgc cggtgacaac gttcacaccg cccgcggtta ttatgaaatt tccgacatcg
     6721 tttgaggcgc ttcgcgccgt atggtcctac ctgtccgttg ttttcatcat ggtgttttgt
     6781 tttagttgga tctacggcag tggtaagaaa gtatttccaa aacttctgca atggatcgtt
     6841 caaccttgtc acgtgcgctg gggtctgttg ggcttgtggg cgatattatc gatcaacaac
     6901 atgttcaaac tcaactatca gacgggcagc gacgtctggg gccatattga ttatatcgat
     6961 tatatcgcga cgaaaggcac actccctctg gctccggaag gctggcagat gtttcaagcc
     7021 ccgctcaatt atctgctgag tgcgccgctt tacgccgtgc tcagcaaatg gctggattta
     7081 ccgtctgtgg ttaaaatcat gacgatcatt ccggtcctct gcgggcttct ccagatagaa
     7141 attgtttacc gcacggcgcg attggttttt acacaaagaa aagatttgca gatcattgcc
     7201 gtcatcgccg gttccctgct gccggttcac acctacgtct gtcagtatgt gggcaatgaa
     7261 cctctgacgg cttgtcttgt gtctgtgctg attttgttga gcgtagaact gattctgccc
     7321 gatcggaagg aacacgggaa cggttatttt gtcgtgatgg gggttgtatg gggattggcc
     7381 cttctttcca agatgactgc tttgctgctg gcgccggtag tgctgctggt gttgattttt
     7441 catgtatggt cgcttcaagg caaattaaaa aacccgttgc gcccggttct gattgttttt
     7501 cttgttccgg tgatcgttgc cggctggtac tatttgagaa attacctgaa actcggcaat
     7561 gcctttatgg ggctatttga tccgctgcaa atgatgcaat ggtggcagga tcccggttat
     7621 cggatatggc cgcatttgct gtccttcggc gaagccttgt acagaccctt ttgtgccggc
     7681 ctgacgagct tctgggacat gttttattcc accttatggc ttgacggcat taattcggga
     7741 ttgatcgatt tcatcccgtg gaatgagaat tttatgattg ccggatctct gttggcttta
     7801 ctgcccagtg tctttatcct gacgggtctg gtaaaccttg ccctgaataa gaagacggaa
     7861 taccgaaacg ccgtcatatt gtccgctgct tcgatcctga tttttctgat ggccatgatt
     7921 gacatgtata ttgcctgtcc ggtgtacagc agaacaaaag ccggctatac gttggggctg
     7981 ctgccctgct atgcaatcct cattgcggcg ggcgcggaac cttttctgcg ccaccgaatc
     8041 attcgttcct ttgcgctggc tttctttgcg tgctgggctt tcgccgcgta cgcggcctat
     8101 tttgtcgtca acgccccata ataatctgct ggaatgtttt tttagcgctc agaaagacta
     8161 ctatgcgatt cattctgccc gtccatgatt ttcaaaatct cgacggcaac cattctgttg
     8221 acttttgcgg aggcgtgtcc gtcattttta ttgacgattc tttccgggat cggcatgttc
     8281 gcggttaagg aagaaacatt aatgacgttt gtaccgttgc tttccagaaa accggtaata
     8341 tcgttaacgt acatcgcgtc gctcattgca aggtcagcca gaaacggaaa aacgacggcg
     8401 ataatttgta tggagttttt tctggcgtaa tcaatgaaca gcatcagatc ttttttatgg
     8461 tcggacaaaa ccttattatt tttgtatgcc gtgtcgagat acgtcaaata gggaatgccc
     8521 aggtattctc tgggaaaaag gaaataaacg tagttgaaaa agtaagaacc ggatccgacg
     8581 agcaggagaa accggttcat atcggacggc ggtttaaagc cttcaaatat cagcccgtgt
     8641 ctggcggccg ctccctcaat atcatttccg caatattgca atattatttt ctcgggcttg
     8701 atccggttga ggtaaaggac atgcatcatg gtgtcatatt cttcccggga atccagattg
     8761 ggtttcgcga tgttcatgac ggtaaaatca tttttctttt tattgagttt ctttgccacg
     8821 atgtcggaaa accgttcgtc aacggatttt aagccatggc ctgcggtaaa tgaatcaccg
     8881 acaaataaaa taacatggtt gctttttgcc ggttctttgt ctctgaatcc catggaattg
     8941 atcggtttcc agtattttgc gtaccacagt ttggatgcta aagtgtaatc ggcggaatgg
     9001 gaacggggaa taaacatgaa tgctgcttcc agcacaataa acgttacaaa aaccgccatg
     9061 cagacggttg ccgtgttggc gatgatttta gggatgttgc ggtcaatgaa aaggacttta
     9121 aacaaattga cgcttaatat tagtattact gtaagcagga ataatctgaa ataaacagcc
     9181 gggccggccg gataaaatga cggtaaaata aagacggata ccagcaaaag catgttggtc
     9241 agaattttta ataatatttt tgacatggct ttgtatttat aaattaattt tgtccggaat
     9301 ataagaaaaa tatttttatc attgccatat gaatatagga tctgcctccg gttctgtttg
     9361 cagggacagt ttgcgccggc atgcctttaa gatgcgtgaa tgattttttc tgcatcacgg
     9421 cggcgcttca ggacaaacac catcaaagcc gggcagatct tacaaaaaaa cgataaaggc
     9481 gatgtcgcct ttatcgtttt ttaagtcatt gacaaaaaac gtaaaattaa aacttgcgaa
     9541 tcggccggac cagccgtccc tggctcttgt acgttccggt ttgtgccccg ttgctgaaat
     9601 actgagacca cgcatcccac tggctgtatt ccgaggaact ccagtaccct tcatcgagaa
     9661 atcctccgga cctcttcacg tcgcggttgg tgaataactg ggccagttcg tccctggaag
     9721 gcagaaacca gtcgttaaac agatagtctc ctgcgccaac gctgtaatcc agacataatg
     9781 ctgccgcata aggaatatcg gtgccgccgg cctcttccac ttgcgctaca atggcattac
     9841 tgttggccaa gcctgttccg attgcctctg aggtgtttcc gttggttgtg gattgtgttt
     9901 tctggtaaat atcgtcattt tcatcaacct ggggatcccc attaatccat gccacagaag
     9961 atgcctcttc actgccctga taccagttcg gcggtgcagc ctccagaccg tgcagtcctc
    10021 cgtcggttac ataaaatacc ttgccgacac agctgggtcc gtaatcacct acctcaaacg
    10081 gagaagtgat cagaggatag tcggctgacg ggtaatccca atcccatttt gcgtaaagtg
    10141 taattttgcc ctcaacggta ttgctgtcat tcaggacggt atctgctgtg aaggctgaac
    10201 cttcgccgtc ggattccgtg taccatccgg caaatttgta acaccgcttg gcaggttccg
    10261 gtaaaatccc ttcttcagtt ccggtggcga cggtattgcc tttcgataaa atgacatatt
    10321 gatcgttgat ggaagcgccg ccgtagctgt tatattccac tgtatagccg gaatcggaaa
    10381 cccattttgc ataaaccgtg atattttcgg tgaccggcgt gcttccggtg aacgccgtgc
    10441 ctcccccgcc aacggccgtg taccagccgt caaacgtatt gaaaactttc gtggggtctg
    10501 ccggcaaggt accgacgttt gttgccggtg cgacaacgta ttgcgttcct gccgcggttc
    10561 ctccgtcact attgtaagta acgatatgta ccgtaccggt ggcccatctg gcataaacgg
    10621 taatatctgc cgtaacttcc gtaccggcca aaaattgaga gccgcccccg tcgggtgccg
    10681 tataccatcc tgcaaaggta tacccggtct tggtgggaga tgcgggcagt gtcgagaggg
    10741 ttttggaagg cggggtgacg cctctgctgg cgtagacggc gccatcactc ataaagataa
    10801 cctgatattt ataccagaac gcataaacga tcatatccga attaacggca gtggctgctg
    10861 taaataatgt tcccgtgccg cctttaccgg tccaccagcc gccgaattta taaccggaca
    10921 tgctgggagc cgtcggcaat gttccaacac tctggccctc ttccacagta atactggtcg
    10981 gattgggcat cacggttgta ctttcgggat cattgaactg actgttaaag gcgacggtgt
    11041 aatttgtcgg tggggcatcg tccaaactgc tgcaaccgct atacacgata gcggtgaata
    11101 aaatgataaa aacaggaata atctttattg aaagtttcat ttttcctcca aaacaaaatg
    11161 tttttattag aaaataatac ctgtccgttg aggctgaaat ttttttagat tgttgaaatt
    11221 aatacaaata caggtttttt gtcaatcaat ttattgtcca ataaaggatc gtgataaaac
    11281 cctgccttct tttccatgac cgggaaatgg caccggaaga caaaacatat cgtttatgat
    11341 gaaggataca tcatgcaatg ttgtgtccgc aatggctctg agtcagtgac ttctcccctc
    11401 cggtctaaac gttgaagcgg aaagtaatga tgtcgccatc ctgtacaata tattccttgc
    11461 cttccagccg caggcgtccg gctgatttta cctgcgcggc ggaaaatccg tgggcaacaa
    11521 aatcgtcata atgcatgact tccgccttga taaaaccccg ttcgaagtcg ttgtgtatag
    11581 ctccggcggc tttgggcgct ttggtttcgg caggaatgac ccaggccttg acctcatcct
    11641 cgccgacggt aaaaaaagaa atccgtccga gcaaggaaaa agctgcgcgg atgatgcggc
    11701 cgaaagccgg ctcggcgatg tccatcgacg ccagaaattc tttctgatcc gcggcgtcca
    11761 gaccggccaa ctccgcctcg attttacagc agattcccat gaccggttct tttaatcttg
    11821 ccgctgcggc gaattgttcg gcaaatgata aattgtcctc cgctgtattg acgacaacca
    11881 actccggctt gattgtccag aaggaaaagc tcttcagtgt aaaaatttca gtaggcgtta
    11941 aacccgccgc acgcaacggt tttccggctt ctagacaatc ttttaagcgc ggcaataact
    12001 cgttttgagc ggcttcctgt gcgggaatgg ctttccgggg catttttgca agcttctcca
    12061 tctttttttc aatgattaat aagtcggaaa gtaccaattc atcttccagt ttgtgatagg
    12121 cttcgatact caactgaata tcgggcaggg aaaaaccgtc aacgacatgc agaatcccgt
    12181 cggtgccgct taaattctga cggatggcct cccacggatg ttcgccaacg gcgtgtacgt
    12241 cgacaaaagg cactttggcc ggagaaattt ttgccggttt gtatatttca acaagtttat
    12301 caaaacgttc gtcaggcacg gaagcttggc cgcgcaagac ggtttcgttg tgcgactggt
    12361 tgcttttcac gccagtcata atttcgaata aggtactttt tccgctttgg ggaagaccca
    12421 gtatgcccat ttccataaca aatctctttt ctacttaaaa aaattattta gaataaccaa
    12481 actaatgttc ttgtaagaaa gtcaaaaaat ctataacatc gtcattccgg cgaaggccgg
    12541 aatccagtat tgataagctg attcggaccc cggcttccgc cgggaatacg acatttggtg
    12601 tttatttcag tttaaaatca gccctgctat tcttcacttt ttcttttttc tccggtgtca
    12661 gacttttcga ctccacaata aatatccggt ccgcggcgat atccgactga agcatcagct
    12721 cttttacgtt ttgcgcgcgc cgggcggcaa gctgacgcag gtcgctgtcc gtcacgtcaa
    12781 tattggccag cattaatttt tccatctccg cgggcggcaa cgttttttgc atgcccagaa
    12841 tattgcgcgg tttggaaaaa tccgccgatc ggtaaacctg tttcaggtac ttatcatatt
    12901 cctgttctgt aagcaccacg ttttcaacgg caatctgttg attatctttt gacatcgttt
    12961 ctttcatttt ctgcgctttg atacggcggt caagctccgc tttcttcaaa gcatatttgt
    13021 cctgttccgc gtccacgtag ctttcgatat ccagtttgat gttggggcga tcattcaggg
    13081 ctttgccgat catgctgatc ttttttgttt cttccgcgct gatcacggcg cttccgtagt
    13141 caaattcgat aaaactcaat tcctcaccgc cgccggtaag agaggaaagc agggcaaagg
    13201 gggaggtgac cgccttggta atcagattga cgataatctg ccagataacc gaccagacct
    13261 tgaatttcgg atcgtccaga cttcccgata ccggaagatc caggttaatc tgtccgttac
    13321 ggtcggttag cagcgttgcc gccagcgtca ccggcgcttt gacagcttgc ggactttcca
    13381 ctttttcgcc gaaggtcaac tggtcaataa gcactttatt ttgggagtcc aattttcttt
    13441 tgtcgatcaa atatgaaacg ttaaacgtca gtttgccctt ggtgatggga tatccgagga
    13501 atttgttgga atagggggtg atggggctta actcgatgtc cttgaaaccg acttttaaat
    13561 cgacgaagag atccttggcc agcggattaa ttttccccgc gatttcaatc ggcgagccgt
    13621 atcccagatt ccccttgagg gccacatcgg cgcgggaaaa ttctttcgag gaaagtccgg
    13681 taacgcttcc ggtcagattg aacatgttcg ccgaatagtt cggctgaatg ttattatcgc
    13741 tgaaatcgat gtgaccgttt ttgaatacga ccttgccgat tttaatatcg gcagtttcct
    13801 ggttatcttt cttctttgtt tccgcagtta ccggcttctt ttcttctttt ttgatcgctg
    13861 tttcttttga cgcttgatta tcaccgaata tatcctgaac attcaatgac gcatcgggat
    13921 tgatgataat ccgcgtataa aaatcagcaa gcgatatggt gttgatgctc agagaaaaag
    13981 gattgtagcc gaatttgatt ttatccaggg agagcctttt gaatttcaga aaatcattgg
    14041 attgcgcctt gtccagcgcg gccaggttgg agacggaaac agaccctgtg catttaatga
    14101 ccagttcatc ttttttgttt ctgctcaggg aaaaatttcc cgttgatgaa atcgttccgc
    14161 gcgtgatatc cattttaacc ttgtcggtga aatacggttg aaacgcccgg atgccgatat
    14221 ttttccaatc agactgcaca tccacactca gcggctccaa ggtcagcgtt accctgtctt
    14281 taaactcacc tttcttgttc aaaatgcctt ccagctcaac ttttcctgtt gctccttttt
    14341 ccgtggaaag attggcggct ttaaaactca gcggtgaaaa atgaagtttg accggatcgg
    14401 agggttgaga atccatgtaa ataatatcgg ctttatacag caacacatca tccacgcgga
    14461 atttaaaccc ggatttcttt ttagatgcag cggcagcttc tttttgcggc aatttttcta
    14521 cttgcttctt tgtcagattg agaagattga tttcaccttc tttatcacgg cgtatgacga
    14581 gttccggaga ttcgatggcg atacgcgcca gatgaatatt cgatacaaaa ggttcgacag
    14641 aggtcatatt gacgttcaaa gaaggcaaat gaagaatttt attgtttttc aggtcatcga
    14701 gaatcacatc tctcaaaacg acattgccgt taagagtcag tgccggggat ttatcctcat
    14761 tgacaataaa attaagtttt atattggtgt cgagccgcgc ggatttgagc tgacagttca
    14821 ttttcgccgg cacatagttc agataatagg ggatgtcgat atcctgaata ttgatgtcga
    14881 aacttgtggc gcgcgaggtc agaaagggct gtgttttacc ggcaagctca aatttgttat
    14941 tgttgatatt agtggaaaac ttcggttcca catatttttc cagatgatat tcgatattgg
    15001 atatgaaggg gacggagaca ttgagttccc gcacggtatg attggttttc ttcggcgcgt
    15061 cttcaaaatc aatattgccg ttaataatct gaatgttgtt caacgagaaa agaaaaggtt
    15121 tgtcttcttt gtccggtttt gcttcatcct tgggaagcag gtctgagaaa ttgtaggagc
    15181 cttccggttt gcgggaaatt ccgacgtaag gattcgtcag cgttatttgc ttcaggatca
    15241 gagcgcggcg gaaaagggag gacatcaaat cggcattgac ataaagttcg tcaaacgcga
    15301 caaaagcttt tggcttcccc ggatcttcca gtgaaaattt ctttaatgtt atggaaacag
    15361 cgtaaggatt gatgctgatt ttttcaatgg acgctttgcg gtgcagcgcc tcggacatct
    15421 tcttcatgat gtacggcttc atcagcggcg gcgcgataaa aaatccggca attcccagaa
    15481 caaccagaaa aataagaaga ccaatcgcga
//
LOCUS       DAONQZ010000053        14808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4505_length_14808_cov_16.433539, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000053 DAONQZ010000000
VERSION     DAONQZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14808
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4505_length_14808_cov_16.433539"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..1363
                     /gene="mtgA"
                     /locus_tag="PLR13_08580"
     CDS             458..1363
                     /gene="mtgA"
                     /locus_tag="PLR13_08580"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQC19433.1"
                     /translation="MRFFGKIISIILVLFLAYFIIDVGRYFVFPWVGSLAKSSPEKTA
                     FMKHREKMWEKEGVNKKITKIWVPLSRISPYVMKAVIIAEDDKFWSHEGFDFEAMQKA
                     LEKDIKKKKFKAGGSTISQQLAKNLYLSPSKNPIRKVKEAILTWRMERNLSKRRIMEL
                     YLNLAEWGDGVFGIEAAARKHFGKSAAALGPREAAVLAAILPNPIRYSPHGGSKYVAY
                     RSERIYQIMVRRGIVIPEYEEVISETTDESEEETEEQIEESVPEVTEQESAPVQQTEE
                     QKEESVQEEQKATPVLPETEKKSIP"
     gene            complement(1376..2629)
                     /locus_tag="PLR13_08585"
     CDS             complement(1376..2629)
                     /locus_tag="PLR13_08585"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQC19434.1"
                     /translation="MKDVVIVSACRTAIGTFGGTLKNVIGANIAAVVMKEAIKRAGIE
                     PSIIDDVRFGCCLESCNTLNVARVGALLAGIPESVTAVTMNRVCISGMEAVLSGAAMI
                     KAGMADIILAGGVEHMSGTPYVSFDARWGCRLQDTTFIDSMIRALHCGSYVMPLSDDG
                     PVKSGQPFESLKGKPYIMGHTTELIAQLLNISREEMDEVALRSHNNVERATKDGSFKE
                     EIVPIEIPQKKGKPPIIFDKDEHFRPGLTMDQLKALPPAFIPKVGKVTAGNSSGLNDG
                     ASAMIIMSAEKAKELGLKPIARIRSAGRGGCHPSVMGLSPVPAVKNLLKNDPKLTLDS
                     FELCEVNEAFAGQYLGCEKELGLKRDITNINGSGIGLGHPVGSTGSRIMVTLIHAMRK
                     HGKTLGLATLCGGGGVSMATAIEIL"
     gene            3132..3734
                     /locus_tag="PLR13_08590"
     CDS             3132..3734
                     /locus_tag="PLR13_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19435.1"
                     /translation="MNKKIFCLAVALCVSAFFLISCSGGKNAADIAIKAAEEAVNATK
                     AEAAKVVPDEVKSLEDSLAAVKEKFVKGEYKAALEEATALSARAKEVLDAAKAKKEEL
                     TGKWTEMSQGLPKMFEDIQGKVDGLSKLKKLPASLTKEKFEEAKATLASAKDEWAKAQ
                     TSFAGGDFSTAVSMATVLKDKALKIMETLGMSAPGAAPAK"
     gene            complement(3912..4529)
                     /locus_tag="PLR13_08595"
     CDS             complement(3912..4529)
                     /locus_tag="PLR13_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQC19436.1"
                     /translation="MEIILGIAVGIGLSAACGFRIFVPLLIMNLAALSGQIQLPAEFA
                     WIGGTYATIAFTCATLIEVLAYYIPGVDHILDIIATPAAVIAGTITTASMSMDMSPFL
                     KWTLALIAGGGIAGLVQGSTVALRTKSSMTTAGTGNVIVATLELIGAVVIAVMAILLP
                     VVCLVIIVLFIAFAFKKVGVFIFRRFRKPIKQTDKNAFSRRLPRG"
     gene            complement(4563..5900)
                     /locus_tag="PLR13_08600"
     CDS             complement(4563..5900)
                     /locus_tag="PLR13_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQC19437.1"
                     /translation="MKDIMKKIRQLANFIAHDIWRIRRSQLSAGKSFLIKQARVLILS
                     LKGFHEDKCQLRASALTFYALISIVPVAAMAFGIAKGFGLKEALETQLRSRLAGQEEI
                     AANIIAFSHSLLEKTQGGLIAGVGLVVLMWAVFKLLGQIESSFNDIWKIKEKRSIGRM
                     FGDYLALIIICPVLLILSSSTTVFITTQVSVILEKFTVLGCFSSFIFFLLKLLPYMLM
                     WFLFTFLYIFIPNARVRFSSGLVAGIIAGTIFQIVQWAYITFQIGVVHYNTIYGSFAA
                     LPLFLVWLQLSWTIVLYGAEVSVAYQNADTYEFEPDFLQASHRMRILLSLLVTSRVIK
                     NFIRGEKPMTALDISSQLGIPIRFVNEILFDLIKSRIFSVVEIEGEDRRGHQPAIDVS
                     GLTIQDVMDALETRGMNTLPLSDQDGLKSLADSMDAFFRKIETLPENRCLKDI"
     gene            6128..6712
                     /locus_tag="PLR13_08605"
     CDS             6128..6712
                     /locus_tag="PLR13_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19438.1"
                     /translation="MHKIFFRKMNEKWKTFFFLALIFAISSCYYYPQEQMVTRPSPDE
                     NIRAITQIYFYPNRNQSADLQSRDHYACYTWAMEQTGFDPSESSIVPEQRVRVVPMPP
                     PGQDTIAMSIAGAVLGALIAGPRHAGGGALIGAAGGAMAGMVSDASRVEAARQMEEAY
                     NNRNQGRDFRREKKGLQFRRAMSACMEGRGYTVK"
     gene            6737..7483
                     /locus_tag="PLR13_08610"
     CDS             6737..7483
                     /locus_tag="PLR13_08610"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HQC19439.1"
                     /translation="MISVKFNFKKCFVVVLSLFMLTFVSELVFTPASQAQPSRKSSVR
                     TVKKGSTVHHRKQFFDARHSHNRAYPVRGHYFRSLPRDHRVIRHGSSRYYSTSHGVWY
                     RPYRGLYEVIAPPIGIFVPFLPLFYTTVRVHGAPYYYANGTYYTQTPGGYVVVHPPQG
                     EVMENPPVTDDEDEGFMDDKLFIYPSKGQSEKQRDLDRYECHQWALEQADYDPTQISS
                     GRSADEVMQGRVEYRRAIAACLESRGYAVK"
     gene            7565..8050
                     /locus_tag="PLR13_08615"
     CDS             7565..8050
                     /locus_tag="PLR13_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19440.1"
                     /translation="MERQKILQQMIDLNKIFFDSWFSMMAMFQHQTEEILKPFVDCMP
                     GMDGRMKKFIRHWSEECKKNRETFKRDIDAGYAKAEAFFDYHAILKFQEQNEQLLNDF
                     LSQTTWMPDDFKTAAEKMADVYKDGCDKFRRYVEKNINGVSDFSSSSGKPGRKAGSRK
                     K"
     gene            complement(8102..9538)
                     /locus_tag="PLR13_08620"
     CDS             complement(8102..9538)
                     /locus_tag="PLR13_08620"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQC19441.1"
                     /translation="MMKVVHRALILFLTVVFPAVVFAGGFGLDSSTADIGTLLPLWTT
                     LPFTGILLSIALIPLLLPRFWHRHFPKVSAFWAIVFAVPFIFFFREKALHEIAHIILI
                     DYVPFILLLWALFTVSGGIYVRGTLKGTPAVNTTVLLIGTLLASVIGTTGASMLLIRP
                     ILRSNAWRIHKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTMHIFPEMA
                     VASVTLLLIYFALDSHYYKRENKTAIPEEEFEPLKIEGGRNFIFLAGIIGAVLFSGSV
                     RLGDVNILGIHQTTENLIKDAVLILMGLASLGFTAKEVRKNNDFGWAPMLEVAYLFAG
                     IFITIIPALAILKAGEKGALHWLIKAIDSPAQYFWAVGILSSFLDNAPTYLTFFNSML
                     GKFYPGMPEPAAVALLIVEKIPYLAAISVGAVFMGANTYIGNAPNFMVKSIAEEAGVK
                     MPSFFGYMFKYSIPVLVVLFIVMTFVFF"
     gene            complement(9535..10596)
                     /locus_tag="PLR13_08625"
     CDS             complement(9535..10596)
                     /locus_tag="PLR13_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQC19442.1"
                     /translation="MNLMQESYLGNTLQAWLIALSILIVVFIILKLIQAVAVRKLSRL
                     AVSTSNQIDDLLVGLLKRTKGFILIIAAAYLASLTITLKPSVGQLWQKVVILALILQA
                     GFWASSAIAFAIGNIIKKRSEEDFSSKTTITFLGFVARFVLWIVILLLILDNLGVNIT
                     GLVAGLGIGGVAVALAVQNILGDLLASLSIVLDKPFVIGDFITVDSLSGTIEHIGLKT
                     TRVRSLSGEQLIFSNNDLLKSRIRNYRRMSERRIIFNFGVVYQTPLEKLKTIKMIVRD
                     IIDKTETARFDRVHFKEYGDSSLNFEAAYFVQSSDYSTYMDVQESINLEIFRRFRDEG
                     IQFAYPTRTVFIQDEKQQK"
     gene            complement(10672..11082)
                     /locus_tag="PLR13_08630"
     CDS             complement(10672..11082)
                     /locus_tag="PLR13_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQC19443.1"
                     /translation="MLNFKNILYPIDLNSEKISSLETALEFARLLNGALHIFYVNDIL
                     AGYRHPTDHEDAVALRVKETVDASLLENINIIYAVSKGETTEEIVKYAQDNQIDLIII
                     GHKHRGRLYASLFDSTDIDTIDKVFLPVLVIPEK"
     gene            complement(11403..12170)
                     /locus_tag="PLR13_08635"
     CDS             complement(11403..12170)
                     /locus_tag="PLR13_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQC19444.1"
                     /translation="MGEAVTKEIRDGVAILAIDKPKMNQLSGEVFEAMRKHLDAVEAD
                     KAIRCVIITGAGEKAFSAGADLASGFGDFSAVDFLKRGQDIWNKVEYFPKPVIAAING
                     HALGGGCELAMACHIRFMKKGARIGLTETNLGIMPGYGGSLRLPRLVGKSKALEMIYL
                     GKQCEADEALSIGLVDHITEGNVVDAALELANKIVKRAPLAVAAVTKLCNISPSISAE
                     IHLKIEREELGKLFNSKDAQEGIGAFFEKREANFKGE"
     gene            12737..13504
                     /locus_tag="PLR13_08640"
     CDS             12737..13504
                     /locus_tag="PLR13_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQC19445.1"
                     /translation="MAYDNIIVETKDFVTVVTLNRPPMNSINLGIREELDQIITDVQT
                     SKETRVVVVTGSGEKAFCAGMDVADVANIDKGPNGIDVCNKVERSLKPVIAVINGHAL
                     GGGCELAMACHFRFMTDNPKSQIGCPELNLALTPGWGGIQRMPRIIGKSKALDFILFS
                     KKVSGPDALAIGLVDKVFPAADLMKEAMHFAQALSKRAPIAVNAVLNGMNIGLEKGIV
                     AGLEADKQWIEKLKTSKDATEGVVAFIEKREPNFVGE"
     gene            complement(13749..14657)
                     /locus_tag="PLR13_08645"
     CDS             complement(13749..14657)
                     /locus_tag="PLR13_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC19446.1"
                     /translation="MSNISFEGKVVIVTGAGSGLGREYALEFARRGASVVVNDLGVAR
                     DGSGSTKNAADAVVTEIKNSGGKAVANYDSVATPEGGINIVKTAVDTFGTVDVLVNNA
                     GFLRDKSMMKMTADEWDAIMSVHLRGAFCVTQPALAVMREHNYGRIMVTSSTSGIFGN
                     FGQINYGSAKMGLVGFMNVLKLEASKFNVHVNAIAPTAYSRMTENLIPKELAPKLQAK
                     FNVPMVVYLCSEECQENGSIFTMGAGWYAKTAIVCATGACLGDGSRDIPAEEVRDNFA
                     KISDLTGAKPLYDSMGIFELMGPLLQ"
BASE COUNT         4036 a         3421 c         3677 g         3674 t
ORIGIN      
        1 ctaagaccga acaacaaaaa aaggacaaag agtcccgaga atacagtcat ctgccgtttc
       61 ataatgaaat gcctcctgtt ttatgggggg tattatccta ttaacggtgt acatgtttcg
      121 atttattatc attgctttaa ccgaaaaaac ttataacctg ttgttgtgca cacctgttgt
      181 gcattttttt ttgactcatg cacaattaac acataatttt ctggactctt actaaaaaaa
      241 aagccgtctg tcaatttttt ttacagcagt ggatgatctt gtaacagcaa atagtgaatt
      301 tctatggcaa aaagtgtttt gcgccgacaa agtaaattat tattggattt caaaacgatt
      361 ttcggttatg gcagtgcctg ccgtcagggg atttccggct gaaaaccgga ggtatcaaaa
      421 gacgatggaa gacggtaatt tttttaagag ggctgttatg cgatttttcg ggaaaattat
      481 ttctattatt ctggttctat ttttagcata ttttattatc gatgtggggc gctattttgt
      541 ttttccgtgg gtggggtctc tggcaaaaag cagtcctgaa aaaacggcat ttatgaagca
      601 cagagagaaa atgtgggaaa aagaaggcgt caacaaaaaa attacgaaaa tctgggtgcc
      661 tctatccagg atatcgcctt atgtcatgaa agcggtcatt attgccgaag acgataaatt
      721 ctggtctcac gaggggtttg attttgaggc catgcagaaa gccctggaaa aagatattaa
      781 gaaaaagaaa ttcaaggccg gcggcagcac gatttcgcag caactggcaa aaaatctgta
      841 tctctccccc tcaaaaaatc ccattcggaa agtcaaagaa gccattctga cctggcggat
      901 ggaaaggaac ttgagcaaaa gaaggattat ggaactttac ctgaatttgg cagaatgggg
      961 agacggcgtt ttcggtattg aagcggcggc ccggaaacat ttcgggaaaa gcgccgccgc
     1021 cctgggaccc cgggaagcgg cagttctggc agcgattctg cccaatccca tccgatatag
     1081 tcctcacggt ggatcaaaat atgttgcata ccgttcggaa agaatttacc agatcatggt
     1141 gcgccgcgga attgtcatcc ctgaatatga agaagtgatc tctgaaacga cggatgaaag
     1201 cgaggaggaa acggaagaac agatcgagga aagtgtcccc gaagtcaccg aacaggaaag
     1261 tgcaccagtt cagcagacgg aggaacaaaa agaagaatcc gttcaggaag aacagaaagc
     1321 aactcctgtt cttcctgaaa cagaaaaaaa atcaattccc tgaggggaat ctctcttaca
     1381 atatttcgat agccgtggcc atggaaacgc ctccgccgcc gcaaagcgtg gccagtccca
     1441 gcgtcttgcc gtgtttgcgc atggcatgaa ttaatgtaac cataattcga gagccggtgg
     1501 agccgacggg gtgacccagc ccgatgccgc tgccgttgat attggtaatg tcacgtttta
     1561 agccgagttc tttttcgcaa ccgaggtact gcccggcaaa cgcttcgtta acttcacaca
     1621 attcaaaaga atcaagggta agcttcggat cgtttttgag caggtttttc acggctggaa
     1681 ccggacttaa tcccatcacg gaaggatggc aaccgccgcg tcctgcagaa cgtattctgg
     1741 cgatcggttt cagtcccaat tccttggctt tttccgccga cataatgatc atggcggaag
     1801 caccgtcgtt gagccccgag gaattgccgg ctgtcacctt gccgactttg ggaataaatg
     1861 ccggaggcag ggcttttaac tgatccatgg tcaaaccggg gcggaaatgt tcatccttgt
     1921 cgaaaatgat gggcggcttg ccttttttct gggggatttc aatcggtacg atctcctctt
     1981 tgaaactgcc gtctttggtg gcgcgttcga cgttgttgtg gctgcgcagg gcgacctcgt
     2041 ccatttcctc tcggctgatg ttcaaaagct gagcaatcag ttccgttgta tgacccatga
     2101 tatagggttt gccctttaaa ctttcaaaag gctgacctga tttgacgggg ccgtcgtcgg
     2161 acagcggcat gacgtaggaa ccgcaatgca gagcgcggat catggaatct atgaaagtag
     2221 tatcctgaag gcggcagccc caacgggcat caaaacttac gtagggtgtg ccggacatat
     2281 gctcgacgcc gccggccagg ataatatcgg ccattccggc tttgatcatg gctgcgccgc
     2341 tgaggacggc ttccatgccc gaaatacata cgcgattcat ggttacagcg gtcaccgatt
     2401 ccggaatacc ggctaaaaga gctcctacac gcgccacatt aagtgtatta caggattcga
     2461 gacaacagcc gaaccggaca tcatcaatga ttgacggttc tatccctgcc cgtttgatgg
     2521 cttccttcat gacaaccgcc gcgatattag caccgatcac attctttaaa gttcctccga
     2581 atgttcctat cgctgttctg cacgccgaaa caattacaac atccttcatc gttcaaactc
     2641 cttatcgtta tgattatttt cttctataaa atctgaatga ctcagacaga ctttttaaga
     2701 agtaatttct tcaactggaa tgtacacctt caagcttttg aagccattga tctagcttat
     2761 tttctaaaaa ataaacaaaa ccgaccactc catatcttat aatccatctc aaagtcaaga
     2821 ccatcgatgt cctgaaatga aaagtttgag caaaatattg cattaagaaa tatgatagta
     2881 atttatgtaa agtatggaaa aggtcttaat gaagggtgat ttgcgccgca ataactttaa
     2941 aatccttgat cttttcagtt gaccggccct gaaaaaatga ttaataaggc agaaaaaagc
     3001 aattaaggag gtgacgccgg gaagcaaatt aaagaaatcg aaaagaattt aataaaaaac
     3061 gtgcggtaca aaacatctga caaaatttta aaaagagtcc atattttaaa aatatgaaag
     3121 gatgaacggc tatgaataag aaaatttttt gtttggcggt tgcgttatgt gtaagtgcat
     3181 tctttttgat ttcatgttcc ggcggaaaaa acgcggccga catcgcgatc aaagcggcgg
     3241 aagaagccgt taacgctacc aaggcggagg cagctaaagt tgttccggac gaagtcaagt
     3301 cgctggagga ttcacttgcc gctgtgaagg aaaaatttgt taaaggcgaa tacaaagccg
     3361 ctctggaaga agcgactgct ctttccgcca gggccaagga agtgctggat gcggcaaaag
     3421 cgaagaagga agaactgacc gggaaatgga cggaaatgag ccaggggctt cccaagatgt
     3481 ttgaagatat tcaaggcaag gttgatggcc tttccaaact taaaaaactt ccggccagtc
     3541 tgaccaaaga gaagtttgaa gaggcgaaag ccactctggc ctcggccaaa gacgaatggg
     3601 ccaaggccca gacgagcttt gccggcggcg atttcagcac ggcagtcagc atggccaccg
     3661 tgttaaaaga caaggctctg aagattatgg aaactctggg aatgagcgct cctggagcgg
     3721 ctccggcaaa atagtcaacg gattcgaatc ggatggaagg gatatgctcc ccatttgtca
     3781 gggagcatat ccctttttta tggagatcct tttgaaggca tgaaatcgtg cattggaatc
     3841 atcgggcagt ctcaggtcgg acaggatgtc gggttctcat ctcctgttgc gtgcaggaca
     3901 cacgaacgtg atcatccccg cggcagtcgg cgggaaaatg catttttatc ggtttgcttt
     3961 atcggttttc taaatcttct gaatatgaac acgccgactt ttttaaaagc aaaagcgata
     4021 aataacacga tgatcaccag acaaacaacg ggcagaagaa tggccatgac ggcaatgacg
     4081 accgccccga tcagttccag cgtagcgacg atgacgttcc cggttcccgc ggtcgtcatg
     4141 gaggattttg tgcgcagagc gacggtcgag ccttgcacca gcccggctat accgccgccg
     4201 gcaatcagtg ccagggtcca tttcagaaaa ggcgacatgt ccatactcat ggacgctgtg
     4261 gtgatggttc cggcgatgac tgcggcaggt gtggcgatga tatccagaat atgatcaacg
     4321 ccgggtatgt aataggcaag cacctcgata agggtggcac aagtaaatgc aatggttgcg
     4381 taggtgccgc cgatccaggc aaattccgcc ggcaactgaa tctgtccgct taaggcggcc
     4441 agattcatga tcaacagcgg aacaaaaatc ctgaatccgc atgccgcact caacccgatg
     4501 cccaccgcta ttcccagtat gatctccatg attattcttg ttaaggtcct gtcataatga
     4561 atttaaatat cttttaagca cctgttttcg ggaagcgttt caatttttct gaaaaaagcg
     4621 tccatggaat cggccagtga tttgagcccg tcctgatcag agagggggag cgtgttcatc
     4681 cctcttgttt ccaaagcgtc catgacatcc tgaatggtca ggccgctgac gtcaatcgcc
     4741 ggctgatgac cgcgcctgtc ttccccctcg atttccacaa cagaaaagat ccggcttttt
     4801 atcagatcga aaaggatttc attaacgaaa cgaatcggga ttcccaactg actggaaatg
     4861 tccagtgccg tcatgggttt ctctccgcgg atgaaatttt tgatgacacg actcgttacc
     4921 agaagcgaaa gcagaatccg catgcggtga ctggcctgaa gaaagtccgg ttcgaattca
     4981 taggtatctg cattctgata ggcaaccgat acttccgctc catataaaac aatggtccag
     5041 ctcagttgga gccagaccag aaaaagcggc agcgcggcaa aacttccgta gatggtatta
     5101 taatgaacga caccgatttg aaaggttatg taggcccatt gcacgatctg aaaaatggtt
     5161 ccggcaataa taccggcaac aaggccggag gaaaagcgaa cccgggcatt cggaataaaa
     5221 atataaagaa aggtgaaaag aaaccacatc agcatatagg gcagaagctt caacaaaaag
     5281 aagatgaaag aactgaaaca tcccaggacg gtaaattttt ccagaatgac actcacctgc
     5341 gtcgtgatga atacggttgt gctgctggag agaatgagca ggacgggaca aataatgatc
     5401 agagcaagat aatcgccgaa catgcggccg atcgaccgtt tttcttttat tttccagata
     5461 tcgttgaaag aagattcaat ctgaccgaga agtttgaaaa cagcccacat aagaacaacc
     5521 agaccgacac cggcaatgag gccaccctgg gttttttcca gcagagaatg ggaaaacgcg
     5581 atgatattgg cagcgatttc ctcctgaccg gccagcctgc ttcgcagctg tgtttccaag
     5641 gcttctttca gcccgaagcc tttcgcgata ccgaaagcca tggcggcaac gggaacgatc
     5701 gaaatcagag cataaaaagt cagggcggaa gcgcgcagct gacatttatc ctcatgaaaa
     5761 cctttgaggg aaagaatcag aaccctcgct tgtttgatca ggaaggattt gccggcagaa
     5821 agctgggaac ggcgaattct ccagatgtca tgggcgatga aattggcgag ctgtcggatc
     5881 tttttcataa tgtctttcat gatggcgctc cgcgaatccg gctgaaagat aattttcaga
     5941 caggaagata agaataaaga taacagatag atatgttaat cgcaaaagat ttttcatccg
     6001 tgttgaggca gcataagccc aaagggctca ttttgattaa tgattttaat tgatttaatt
     6061 aaaaattgaa aagtgctatt attgtaaaac agccgtaaaa aaattaacag gaccagggag
     6121 ggaaaacatg cataaaatat ttttcagaaa gatgaatgag aaatggaaaa catttttttt
     6181 ccttgccctc atttttgcaa tcagttcctg ttattattat ccacaggagc aaatggtgac
     6241 ccggccgtct cctgatgaaa atatcagggc tattacgcaa atttattttt atcccaaccg
     6301 taatcagtcg gcggatttgc aatcgcgcga tcactatgcc tgctatacct gggccatgga
     6361 acaaaccggc tttgacccca gcgaatcctc cattgtgccg gaacagcgtg tccgcgttgt
     6421 tcccatgccg ccgccgggac aggataccat agccatgtcg attgccggag cggttctggg
     6481 tgcgttaatt gccggaccga gacacgccgg cggcggggca ttgatcggag cggccggtgg
     6541 cgccatggcc ggaatggttt ctgacgcttc ccgggttgaa gcagccaggc aaatggaaga
     6601 ggcgtacaac aaccgtaatc agggacgcga tttccgcagg gagaaaaaag gcctgcagtt
     6661 tcggcgggcc atgtccgcct gcatggaagg tcgcggatat acggtaaaat agttttgcat
     6721 gcataaggag gttacgatga tttcagtaaa attcaacttt aaaaagtgtt ttgtcgttgt
     6781 cctttcctta ttcatgttga cttttgtgag cgaattggtt ttcacccctg catcacaggc
     6841 gcagccttcc aggaaatcgt cggtacgtac cgtgaagaaa ggatcgacag tacaccatcg
     6901 gaagcaattc tttgatgcgc gtcacagcca taatcgcgcg tatcccgtcc gcggtcatta
     6961 tttcagatcg cttccccgcg atcaccgggt gatccgtcac ggcagctctc gttattactc
     7021 cacgtctcac ggcgtctggt acagaccgta ccgcggactc tatgaagtga tcgctccgcc
     7081 gatcggaatt tttgtgccgt ttttgccact tttttacacc accgtccggg tgcatggcgc
     7141 accgtactat tatgcgaatg gtacctatta cacgcaaacc ccgggaggtt acgtggttgt
     7201 tcatccgcct cagggagagg tcatggaaaa tcctccggta acggacgatg aagatgaagg
     7261 tttcatggat gacaaactct ttatctatcc cagcaagggg cagagcgaaa aacagcgtga
     7321 tctggaccgg tatgagtgcc atcagtgggc attggagcag gccgactatg atccgactca
     7381 aatttcttca ggtcgttctg ccgatgaggt gatgcaggga agagtagaat acagacgcgc
     7441 cattgcggcc tgtctggaaa gccgcgggta tgccgtaaaa taatcgctta aagcggtcga
     7501 aaataaaatt ccccataatc ccctttttca tgaaaggggc ctgtcaggcc tggaggaatt
     7561 aacgatggaa agacaaaaaa ttctgcagca gatgatcgat ctgaataaaa tattttttga
     7621 cagttggttc tccatgatgg caatgtttca gcaccagacg gaagagatcc tgaaaccttt
     7681 tgtggattgt atgccgggaa tggatggccg gatgaagaaa ttcatccggc attggagcga
     7741 ggaatgtaaa aagaatcgtg aaacgttcaa acgtgatatc gatgccggct acgcaaaggc
     7801 ggaagctttt ttcgactatc atgccattct caagtttcag gaacaaaacg agcaactgct
     7861 taatgatttt ttgagtcaga caacctggat gccggacgat ttcaaaacgg cggcggaaaa
     7921 aatggcagac gtctataaag acggctgcga caaattcaga cgatacgtcg aaaaaaacat
     7981 caatggcgtc agtgactttt catcctcgtc cggcaaaccg ggaagaaagg ccggatcccg
     8041 aaagaaataa aaattccccg gacaatcaaa ttaatgcatc agccgttttg tgtgtgcaat
     8101 tctagaaaaa gacaaaggtc atgacgataa acaggaccac caggacgggg atggaatatt
     8161 tgaacatata tccaaagaaa ctgggcattt tgacgccggc ttcttccgca atggatttga
     8221 ccatgaaatt cggcgcattt ccgatatagg tattggcgcc cataaaaacc gcgccaacgg
     8281 aaatggcggc cagataagga attttctcaa caatcagcag cgcgactgcc gcaggttcgg
     8341 gcattcccgg gtagaatttg cccagcatac tgttgaaaaa agtcaggtac gtgggggcat
     8401 tatccagaaa actggaaagg attccaaccg cccagaaata ctgggcaggg gaatcgattg
     8461 ctttgatcag ccagtgcagg gcgccttttt cgccggcttt taaaatggcc agcgccggga
     8521 tgatcgttat gaaaattccg gcaaaaagat acgccacttc cagcatcgga gcccagccga
     8581 aatcattgtt tttccggacc tctttggccg taaagcccaa cgatgccagc cccatcagaa
     8641 tcagcacggc atccttgatt agattttctg tggtctgatg aatccccagg atattcacgt
     8701 ctcccaggcg gacactgccg ctgaataaca cagcgccgat aatgcccgcc agaaaaatga
     8761 aattacgtcc gccctctatt tttaaaggtt cgaattcctc ttcggggatc gcggttttat
     8821 tttctctttt gtaataatgg gagtcgagtg caaaataaat cagcagcagc gtgacgctgg
     8881 ccacagccat ttccgggaat atatgcatcg tccagaaaaa agggaccccg tgcagaaatc
     8941 cgaggaacag gggcggatct cccagggggg tgagcgaacc gcctatattg ctgaccagaa
     9001 aaataaaaaa aacgatggta tgcactttat gaatgcgcca ggcattggac cgcagaatgg
     9061 ggcggatcaa gagcatggat gctcccgtgg tgccgatgac ggatgccagg agggtgccga
     9121 taagcagcac cgttgtgttg acagcgggag tgccttttaa tgttcctctg acgtaaattc
     9181 ctccggatac ggtaaataag gcccacagca gcaggataaa gggaacgtag tcgatcaaaa
     9241 tgatgtgggc gatttcatga agcgcttttt ctctgaaaaa aaagataaaa ggtacggcaa
     9301 aaacaattgc ccaaaaggcc gacactttcg gaaaatgacg gtgccagaac cggggaagga
     9361 gcagagggat caacgcgatg gaaagcagta tgccggtaaa gggcaatgtt gtccagagag
     9421 gcagtagggt cccgatgtct gcggttgaag agtccagccc aaaaccgcct gcaaaaacga
     9481 cggcgggaaa aaccacggtc aagaataata ttaaagcacg atggacaact ttcatcattt
     9541 ttgttgtttt tcgtcctgga taaaaaccgt cctggtcggg tacgcgaatt gaatgccttc
     9601 gtcccggaat ctgcggaata tttccagatt gatggattcc tgaacatcca tataagtgct
     9661 gtaatcggag ctttggacaa aatatgccgc ttcaaagtta agcgatgaat cgccgtattc
     9721 tttaaaatga acccggtcaa aacgggcggt ttctgttttg tcaataatat ccctgacgat
     9781 cattttaatc gtcttcaatt tttccagggg ggtctgataa acaacgccga aattgaaaat
     9841 aatacgtctt tcggacatgc gccggtaatt gcgaatgcgg ctctttaaca gatcgttgtt
     9901 ggaaaaaatc agttgttctc cgctgaggct gcggactctg gtggttttca gcccgatgtg
     9961 ttcgatggtt ccggaaagcg aatccacggt gatgaaatcg ccgatgacaa agggcttgtc
    10021 caggacgatg gagagagagg ccagcagatc gcccagaata ttttgcaccg caagcgccac
    10081 agcaacaccc ccgatgccca gccctgcaac caggccggta atattgacac cgaggttgtc
    10141 aagaatcaga agcaggatga cgatccaaag aacaaaacgg gcgacaaatc ccagaaaggt
    10201 gatggtggtt ttactcgaaa aatcttcttc ggaacgtttt ttgataatat tgccgatggc
    10261 gaaggcgatt gcactactgg cccagaaacc tgcctgtaat atcaaagcca gaataacgac
    10321 cttctgccac aattgaccca cggacggttt gagcgtgata gtcagcgaag ccagataagc
    10381 ggcagcaatg atcaggataa atcctttggt ccttttcagt aagccgacca gaagatcgtc
    10441 gatctgattg cttgtggata ccgccagacg tgaaagtttg cggacggcaa ccgcctgaat
    10501 aagttttaaa ataatgaata caacaattaa tatggataaa gcaatcagcc aggcctgtaa
    10561 ggtattgccc aggtaggatt cctgcattaa attcattttt tctccttgtc agctattgat
    10621 gacggttttg aatacggcaa tctctaacgc cgattatcgg attttctgaa attacttttc
    10681 cggaataact aaaacaggca ggaaaacttt atcaatcgtg tcgatatccg tgctgtcaaa
    10741 tagactcgca taaagcctgc cgcgatgctt atgaccgatg atgatcaggt caatttggtt
    10801 gtcttgcgca tatttgacaa tttcttccgt tgtttctccc ttggagacgg catagatgat
    10861 gtttatattt tccagaagcg atgcatcgac ggtttccttg acccgaagag ccacagcgtc
    10921 ttcgtgatcg gtcgggtgtc gataccctgc cagaatatca tttacataga aaatatgaag
    10981 agcgccattc agtaaccggg caaattccag cgctgtttcc agggaagaaa ttttttcgga
    11041 gttcagatca atgggatata aaatattttt aaaattgagc atatctttct ctctgaataa
    11101 ctgtacaact aatataatcg aaaatatatg tcaacctttt ctggtttgcc gggacaggga
    11161 ccatctctga gaagagaaga cattagaaaa tgatgaattt ggtaatattg ttggcgagtg
    11221 ttcaatcccg ctccgctgtg ctcaatgata atttccaatc ccactccgct gcgcttcggg
    11281 gataattcga ccagcaaact tcagtgcact gcgtttctga agtttgggtc tcattaaaaa
    11341 gcctccgtcg cctgatagac tggcaacgga ggctggagat tcgtatcagc agcagctgtt
    11401 tcttattctc ccttgaagtt tgcttctctc ttttcaaaga aggcgccgat gccttcctgc
    11461 gcgtccttgg agttgaagag ttttccgagc tcttcgcgtt cgatcttcag gtgtatttca
    11521 gcggagatgc tcgggctgat gttgcacagc ttggtgactg ccgcgacggc aagcggagcc
    11581 cgttttacga tcttgtttgc cagttcgagt gcggcgtcca caacatttcc ttcggtgatg
    11641 tggtcgacaa ggccgataga gagagcttca tccgcttcac actgcttgcc gagatagatc
    11701 atttccaggg ctttcgattt gccgacgagc cttggcaggc gcagacttcc gccgtagccg
    11761 ggcatgatgc ccagattcgt ctcggtcagg cctatgcgag cgcctttctt catgaagcgt
    11821 atatgacagg ccatagccag ttcgcacccg ccgcccaggg cgtgtccatt gattgccgcg
    11881 atgaccggtt tcggaaaata ttcaactttg ttccagatgt cctgaccgcg tttcaggaag
    11941 tcgacggcgc tgaaatcacc gaacccgctc gcaagatcgg cgcccgcgct gaaggccttt
    12001 tcgcccgcgc cggtgatgat gacgcatcgg atggctttgt cggcttcaac ggcgtcgaga
    12061 tgcttccgca tggcttcgaa aacttccccg ctcaactggt tcatcttcgg tttgtcgatc
    12121 gctaatatgg ctactccgtc tcttatttct tttgttactg cttcacccat gatgatcctc
    12181 cttgtgtgat attgtttggt tgtgctcggc cggttagccg ggcgaactgg taatcaatta
    12241 tcttccgatc attttgaacg agccgttaat cttgccgcgc ccggacttca ggaaatcgaa
    12301 accttccctg accttttccc cgtcgtgatg cagagtcgag ccattccgcg tctcaatacg
    12361 tccggtcaga gaaaacgatt ttctttatgt cattgatgtt gattacagcg tggtcgagta
    12421 taagagacag gtggttgtgt gtcaagggga ttttggtccg ttattgtgaa actcaaattt
    12481 caaaaacgaa gggctttgaa atttgcgggt cgaacaatcc cgccgtaact ggaggggaat
    12541 gagatccgag tttcagaaac agccggccct ggaacttgca gattgaatta tcctgcatag
    12601 gtatggggat aatactccgc ctttggggcg gaagtatagt ccaggacttc ccttggaggg
    12661 ctacgtgatt ttgcttgaag gggaggcaaa aaaatgatta catcattccc acaaacaaaa
    12721 aatcaggagg gtcaagatgg cttacgataa tattatcgtc gaaacaaaag attttgtaac
    12781 ggttgtcaca ctaaatcgtc cgccgatgaa ttccatcaat ctgggcattc gtgaagaatt
    12841 ggatcagatc ataaccgacg tacagaccag caaggagacc cgggttgtcg ttgtgaccgg
    12901 ttccggagaa aaggcttttt gcgcgggaat ggatgttgcc gatgttgcca acattgacaa
    12961 aggacccaac ggcatagacg tatgcaataa agtagaacgc tccctaaaac ccgttatcgc
    13021 cgtcatcaac ggccacgctc tgggcggagg atgtgagctg gccatggcct gccattttcg
    13081 atttatgaca gacaatccca aatcccagat cggatgtccg gaattgaatc tggcgctgac
    13141 ccccggctgg ggaggcatac agagaatgcc cagaatcatc ggcaaatcca aagctctgga
    13201 ttttatttta ttcagcaaaa aagtcagcgg tcccgacgct ctggcgatcg gccttgtgga
    13261 taaggttttc cctgcggccg atttaatgaa ggaggccatg cacttcgcgc aggcgctttc
    13321 caaaagagcg cctatagccg taaatgcagt acttaacgga atgaacatcg gtttggaaaa
    13381 aggcattgtt gcgggtctgg aagccgacaa gcagtggatc gaaaaactca aaaccagtaa
    13441 ggatgcgacg gaaggcgtgg tcgctttcat tgaaaaacgc gaaccgaatt ttgtaggcga
    13501 ataaaatccg attgacgtgc ctcgcggcgg catgtctccg ccgtccgcgg gagatcctgc
    13561 cgtttgcact gccgacgtcg ctttacgctt tgaccggtgt tattcaaatc tgaatctctc
    13621 cagagatttt taagcctccg ttttctttat acgaaaacgg aggcttgtta ttgatgatcc
    13681 tcaatgcgta tgcaggattt tgcgattttt gttcagcgca ttgttcgagg cgatctgtta
    13741 tgtcgacttt actgaagcaa ggggcccatg agctcaaaaa ttcccatcga atcatacaat
    13801 ggcttggcgc ctgtcagatc ggaaatcttc gcaaagttgt cgcgcacttc ttcagcggga
    13861 atatcgcgcg atccgtctcc caggcatgcg ccggtcgcac atacaatagc cgtttttgcg
    13921 taccagccag cccccatcgt aaaaatggag ccgttttcct ggcattcttc cgaacagaga
    13981 taaacgacca tggggacatt gaatttcgcc tgcagcttcg gagccagctc tttcggaatc
    14041 aggttctccg tcatccggga ataggcagtg ggcgcaatgg cattgacgtg gacattgaac
    14101 ttcgacgctt ccagtttaag gacattcata aaacccacca ggcccatctt ggcagagccg
    14161 taattaatct ggccgaagtt tccaaagatc cctgatgtgg aagaggtgac catgattcgt
    14221 ccgtaattgt gctcgcgcat taccgccaac gccggttgag tcacacagaa cgctccccgg
    14281 agatggacgc tcatgattgc gtcccactcg tcggcggtca ttttcatcat cgatttatct
    14341 cttaaaaaac cggcattgtt cacaagaacg tcgaccgtgc cgaacgtatc cactgcggtc
    14401 ttgacaatgt taatgccccc ttcaggcgtt gccacgctgt cgtaattggc aaccgctttt
    14461 ccgccggagt tttttatttc ggtaaccacc gcatcggcgg cgttttttgt tgatcccgat
    14521 ccgtcgcggg caacgcccag gtcgttaacg acaaccgaag cgccccttct ggcaaattcc
    14581 agtgcatatt cgcggccaag accggatccg gcgccggtca caataacgac tttaccttca
    14641 aaacttatgt tactcattgc attctccttt ttattttttc taaactcttt tgggtaactt
    14701 atgcttcagg attatatact gttttgtgta atttgaccgg tttcatttgc ttattgcgca
    14761 tgcgcagatt gagcatttcc acgcccacgg aaaacgccat ggcaaagt
//
LOCUS       DAONQZ010000054        14324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4692_length_14324_cov_18.452940, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000054 DAONQZ010000000
VERSION     DAONQZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_14324_cov_18.452940"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLR13_08650"
     CDS             complement(<1..629)
                     /locus_tag="PLR13_08650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19447.1"
                     /translation="MMSVSVLSVYESKSSRPQGVNKGFFSFDAFNGFDFLALGICLIS
                     FVCSIIEARVNVDNLHWGWAYIAALDLKRGAIPHSEVIIFYGYIYTWIQSISLMLFGE
                     RLVSLGIVTGLFYSVSLFLSYRIFLRLMKKGLACLSVLLIFLIHPYAIYPAPNYFMYM
                     FELLALVYFLKYSESKYYGLLAGFFLSMSVLARYSSVIAVVPPFAVLLCA"
     gene            859..1587
                     /locus_tag="PLR13_08655"
     CDS             859..1587
                     /locus_tag="PLR13_08655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19448.1"
                     /translation="MNINNSNLHKEKIKYSLLLLSQRYNYNRWIYNNIQRFIGNNVLE
                     VGAGIGNLTDFILSKNKLTLIDIHQPYVDYLKAKYSFCDSAAFTVLQTDIQNIQSSPV
                     SGMTFDTVICLNILEHLENDRTAVENMSHLLQPKGKLIILVPALKTLYGSMDISFDHR
                     RRYNKKDLRSLIQDQNLDVLKLYYMNFPGMIGWFVNGRVLKKQELPAGQTKLFDQLVP
                     FFSFAENIVKPPLGQSLILIARKT"
     gene            1624..3027
                     /locus_tag="PLR13_08660"
     CDS             1624..3027
                     /locus_tag="PLR13_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19449.1"
                     /translation="MHFVFVHPPRKIPGGNIWRLINSVNPPLGLALLAALWDREGQTS
                     TIIDAAALQLTIPEIVDQIDPSADFVGITATTPEISSAMTLARDIRNRLPQTRIILGG
                     AHPTVFHRELVKDNICDMAVRGEGEEAITQLARNTPLHLIPNLTWRNTQGETIVNPVA
                     ANYADLDSLPFPAYEKLPMSLYHSALGAAQYQPSIGMVTSRGCPGKCTFCFSEMFGPQ
                     VRFMSPLRVIEHIEVLQKKYGIREISFYDDTFTANKKNVAVLCRLMLEKKINLSWSCF
                     ARVDTVTPDLLQLMKKAGCHQIMYGFETTDEDILKNINKRITAEQFQNAIKWTRAAKM
                     NIRGAFMLGNPGETEASMQRTIAFAKNARIHFAVFNITTPYPGTAMYKEFLRNNSLLH
                     YNWDLYNLAEPVLDLDTVSDNVVRQYYYKSYRDFYLRPIFIFRHIRRIRTLSELLMHI
                     KAVLKIIPLLYRNIFSK"
     gene            3325..4758
                     /locus_tag="PLR13_08665"
     CDS             3325..4758
                     /locus_tag="PLR13_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19450.1"
                     /translation="MKLCKLIKFPDHFKVDISCFHAFLLSCLIALISIMNSNGGILYE
                     EMYTRLPYYLSDIPLLQKLFDSQMLDDGLYRARELSYLFDFIDVKFVELCIEHGYPHF
                     LSLTHYLLCIATGCIIWSFCVKILNLSPLTGIGWLVLFWTSPSVYFSGYFINRTGKVM
                     VMFLTSIIFYFLCKAVVLAKEENSFPISKIDWFLYSISMLIITFLDEQGVFLCMVFFT
                     LLLIWHLIIRQKEFAIMIFVCLATIILHGLYRFFIAPHFIFALNGYRPDFSYQILPMI
                     LYVENLAQYLQAGFFLYLDTFRFLIGNPPLPIGFILLILLIFSPIYFMYANRQRSDNY
                     RKCFGLALGGFLITNVLLVIMNSFMLLKHPPLMWPEARRVFYWLPTTVILVMTLSVWT
                     RLFHKSAVFKFCIVFSICLAVVGNIVALPKHKAIIMRQESYVHSNIQSSSDLLKALKN
                     LYALTDSDDPVIEKNPVFQFFKLKKKD"
     gene            complement(5034..6140)
                     /locus_tag="PLR13_08670"
     CDS             complement(5034..6140)
                     /locus_tag="PLR13_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQC19451.1"
                     /translation="MKKIFFKSLLIIALLIVTFVLPVVYSTGIAVYFRLALLFFIVLE
                     SFKLFKIIIIDAKINHALSNFATTLFSFFIIFMILETVFMFVPRTHFFDYTLASKLWY
                     AKYYKPINSLGFRDNEPNYNHNVILFVGDSFTAGSGLKSVDERFSNIVGKELNKKENK
                     YTVINIGHPGADSSDEFHLMQQVIYMTKIRPEIIVLQYFGDDIAHVAAEKLKTFEGFK
                     PFVNVNAFLKPAVRGSYFLNYMYFLFPNKELTSSALSLMQQTYRDNNMLLKHQEQLKL
                     FINYARENHIQLIAVVFPVLEDIEISNSIYVNDIVNYFEVHKITTINVSRLVKNIPLQ
                     ERIINKNDGHPSKSVHASVAHEVLRKIRFNGNNE"
     gene            6240..6416
                     /locus_tag="PLR13_08675"
     CDS             6240..6416
                     /locus_tag="PLR13_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQC19452.1"
                     /translation="MKHCRSFAVELKRQLVEELLSGMSTPAQLIRRHEKSSGLLYHWK
                     EQYAKGRFNNDPSK"
     gene            6400..6726
                     /locus_tag="PLR13_08680"
     CDS             6400..6726
                     /locus_tag="PLR13_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HQC19453.1"
                     /translation="MTPRSKPPKGVIHHSDRSAQYASHDSVEALLQHGFPISIAGKGN
                     PYDNATTESFFKTLKVEKVYLWEYRTLEDVQIRLPFFIQEFYHRKRLHSSPGYKPPVE
                     SKELFL"
     gene            complement(6958..8391)
                     /locus_tag="PLR13_08685"
     CDS             complement(6958..8391)
                     /locus_tag="PLR13_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC19454.1"
                     /translation="MAKVFLINPSMDILSAFGEYHKLMEPMPCIGLAYIASSCQKAGH
                     EVFMLDNFVEKKPFEDIVESVKSFGADIVGISMLTPTANSTEALGHYIKTRLPSVKVV
                     FGNLHASLFAENLINRGACDAVVHGEGEIIFPRLIDAFLNKSDISKIQGISYKGEEGI
                     VFTGISDPIEDLDALPRPAWELFPWRDYSFLPFVTVAKPCLAISGSRGCPFRCKFCAM
                     GYMGNLVRKRSPESIADEIEWLVKEFGARHIGFVDPIFPLDKKHAIATCRAIRAKNMP
                     EKWWWTCETRVDIVDEEMCREMKLARCKRILFGIESGVNEVLAGVGKGYTTDNVKRGI
                     VAARSAGLEISAFFMLGLPGETKETAKQTIDFALSLDIDFAKFAITIPLPGSELYDDL
                     VKQGKISQPEWESFTTFNPDPDSLPYIPEGMTGKELLQLHRMANVKFYLRPKMIFRHL
                     FVIRSIGLKMMFNGAMLILKQLFKDRK"
     gene            complement(8394..>9016)
                     /locus_tag="PLR13_08690"
     CDS             complement(8394..>9016)
                     /locus_tag="PLR13_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19455.1"
                     /translation="VGRFTTLLAERFKKVVAFDPSLPSLKACQRHLDERGLHNVEMHW
                     ADISFLDNVGENTFDVVIAMGLICYTADPLKSLKRLMRVAKPNAKIFLSVEGCLGALC
                     SQGVEGPERLLNVLSRDPLLLEKDRLINYCNRKDFKELVRNAGVKKLTIDSSHDFGDG
                     AFWQAIDDSRLNDPTYVDMIIRTEDLCRNHSVIAERARIFMAVGEK"
     assembly_gap    9017..9116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9730..10338
                     /locus_tag="PLR13_08695"
     CDS             9730..10338
                     /locus_tag="PLR13_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19456.1"
                     /translation="MNWDFHNILFYPPITGTTILSVDLPPHFRVIAYLIAGIVLLLGT
                     SFFFAKKYSLLNAFRKAIIIAFFSSALFYAIYTDIGWSTWLKTDIKKYWGLSTEEKSI
                     KMYNGLYVFSMEAKKLIHDDYIIYSSYFQEPRLFQYYLLPLNKRSDNHYRLEKAPYIV
                     VLKDTESQYDLAERVFTRGEITIKNVELISMFAHDAYILKRL"
     gene            10335..11792
                     /locus_tag="PLR13_08700"
     CDS             10335..11792
                     /locus_tag="PLR13_08700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19457.1"
                     /translation="MMLSTAIAIFLPTLLGFLIITIILRNDKETFFGERIGLSFPLGA
                     GILTLQIFLLGLMRIPLTLFNSLMPVFFELMLLSFWIWKNKIVLIPSISNPGLSLFLE
                     IKSPRNHGLKKCLFALLIIWIIAKLVSVFILTGLRPIFAWDAWANWSAGAKIFFYSKS
                     LLLDAPALEFFGGNAVDRILYYPLHNTLLQLWMSLWMGKFDDVFVKFFSPVYCLSMII
                     CLYYIAIREIGKLYALALLAIFLSSPLLSYHSIEMYSDQMLGVYLLFASLSFLKAIRQ
                     NLSFCILAGAYATIAVFTKNEALFFVLPFLLSAIVYFRHDLNKSRGKYVNLISILAPF
                     LALLPWFLFKVHYGLGFLGQLRWLRWSHELYFEYYAHSPDTSLWSFFQPDMILVYIYK
                     MISLDNFNVIFLFFPLIILAHGKISKELLHLLVPIAGYMLFFLFLYIFTAYYMGQSMC
                     LTRNILTFYPLFFFLTILLLKKDDTVLSYPAPITR"
     gene            complement(11835..13190)
                     /locus_tag="PLR13_08705"
     CDS             complement(11835..13190)
                     /locus_tag="PLR13_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19458.1"
                     /translation="MEHNVMTENNRLKGKIASIPDYAFLGVILVVCLLYRIYYFGFLN
                     PGMVQYNSDSVSYFYAVDLFRGGVDLYRTPLYPYVIKFFQYVSGDHLVRNLIFFQQAL
                     SFLSIIPFYFVSKRVIGNVYVGMIATLFYGCWHPILIQNVHLNPESLCFAGSVFMLSL
                     LVRFLEKPKRSAALAIGIFPFFLIMLKPTYLILIAVMLLFLLCRFFLLRDERKILYWG
                     LLGLCFTAAGVLGYCGMNKKFNGQFVLSNIALNNTIAHLSISGAYEYGKDEEFMAVID
                     RTRHINYYAAPFSINNHFIDNYKRCYRNFPKDLPPTEDMIFCSVFPDRVNYSPERIIR
                     FISHARCTVVYVKYMAQRAFDIIVAAYGNLFLLLALQSVLIMVAAVKYKKIAWAQSFC
                     IVFVLGQFFSIWLAGLDDMNRHLMPSYPFLIQIAGSFAVNIGLFLSKNKLSSKSDNAA
                     F"
     gene            13404..14195
                     /locus_tag="PLR13_08710"
     CDS             13404..14195
                     /locus_tag="PLR13_08710"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQC19459.1"
                     /translation="MARRLNGKNAIITGAANGIGRASAVLFAREGANLVLADISETGL
                     RQVEDEIKGMGGNVVIRKTDVASEEEIQALINLALEKFNTIDILVNNAGIGGGLDALE
                     DQKPEEWHLVYDINVMGPVYATKHIIAHMKERRRGSIINIASVAGLLSGAGGNAYSAS
                     KAALINFTKTSACEVGAFNVRINAVCPGLIETGMTQPFFDWARSTGKENQLGKYCELK
                     RAASPEEVAWAILFLASDEAGYITGQAIAVDGGITASMSLPGRKI"
BASE COUNT         4157 a         3268 c         2796 g         4003 t
ORIGIN      
        1 gcacaaagca aaacggcaaa aggcggaaca acggcaataa cggacgaata ccgcgccaga
       61 accgacatgc ttaaaaaaaa tcccgccagc aggccgtaat atttgctttc ggaatatttc
      121 agaaaataga caagcgccag caattcaaac atatacataa aataattagg cgcgggataa
      181 attgcataag ggtgaatcag gaaaatcagc agcacggaaa ggcaggcgag acccttcttc
      241 atcaatctga gaaaaatacg ataagaaagg aataaggaca cggaataaaa caggcccgtg
      301 acaatgccca gactcaccag cctctcacca aacaacatca acgaaatgct ctgaatccat
      361 gtataaatat agccgtaaaa gataatgact tcggaatgcg ggatggctcc tcttttcaaa
      421 tccagcgcgg caatataggc ccagccccag tgcaaattgt ccacattcac tctggcttct
      481 atgatgctgc agacaaaact gatcaggcat atgccaagag ccagaaaatc aaatccgttg
      541 aatgcgtcaa aagagaaaaa ccctttattg acaccctggg ggcggcttga cttactttcg
      601 taaacggata ataccgaaac gctcatcatg gcttttgagg caacctcttc ttatggttcg
      661 gttttgtccc gggatacaaa gagagatgtt tatcagaccg ttcaattccg ccttgacaaa
      721 tcaccattcc cgttgttttt taaaagatat tttaaaacgt ttgaattatt taatttttcg
      781 cagatgattg caacattttt ttagatgacc tttatcaatg aataaggtaa tgaagaatca
      841 tccacttaaa aacgggggat gaacatcaat aattcaaatc tccataagga aaaaataaag
      901 tattcattgc tgctgttgtc tcaacgatat aactacaatc gctggatata caacaacata
      961 caacgtttta tcggaaataa cgttctggaa gtcggtgcgg gaatcggcaa tctcacggac
     1021 tttatccttt ccaaaaacaa acttacgctg atcgacatcc atcaacccta tgtggattat
     1081 ttaaaggcga aatactcttt ttgcgacagc gcggccttca cggtcctcca aacggatatt
     1141 caaaacattc aatcatcgcc cgtctccggc atgacgttcg acaccgtgat ttgcctgaat
     1201 attctcgagc acctcgaaaa cgaccggacg gccgttgaaa acatgagtca tcttttacag
     1261 ccgaaaggga aactcatcat ccttgtcccc gccctgaaga cgctttacgg ctcgatggat
     1321 atttcttttg atcaccggcg gcgctataat aaaaaagatt taagatcgct catccaggat
     1381 caaaatctgg acgtgttgaa actttactat atgaactttc cgggcatgat cggctggttc
     1441 gttaacggac gcgtactgaa aaaacaggaa ctgccggcgg gacagaccaa attatttgat
     1501 caactggtgc cgtttttcag ctttgcggaa aacatagtca agccgccgct gggacagtcg
     1561 ctgattctta ttgcccggaa aacgtgattt gcacacaata aaacaattcc gatgattcga
     1621 cctatgcatt ttgtatttgt tcatccgccg cgaaaaatac cgggcggcaa tatctggcgt
     1681 ctgatcaatt ccgtcaatcc gcctctcgga ttggcgttgc tggccgcgct atgggacagg
     1741 gaggggcaaa cgtcgacaat catcgacgca gcggctttgc agttgaccat tccggaaatc
     1801 gtcgatcaga tagatccctc ggcggatttt gtcggcatca cggcaaccac gcctgaaata
     1861 tcgtctgcca tgacgctcgc ccgggacatc cgcaacagac tgcctcaaac cagaatcatc
     1921 ctgggcggcg cacatccgac tgtttttcat cgggaactcg tcaaagataa tatctgcgat
     1981 atggcggtgc gcggcgaagg agaggaggcg ataacccaac tggcccgaaa cacaccgtta
     2041 catcttattc cgaacctgac ctggagaaat acgcagggcg agaccattgt gaatccggtt
     2101 gcggcaaatt atgcggatct ggacagcctt ccttttcccg cctatgaaaa actgccgatg
     2161 agcctctatc attcagcgct gggggcggct caatatcaac cttccatcgg catggtcaca
     2221 tcccgtggct gccccggaaa atgcacattc tgcttttccg aaatgttcgg tccgcaagtc
     2281 agatttatgt cgccattgcg cgttattgaa catatcgaag tgttgcagaa aaaatacggc
     2341 atcagagaga tttccttcta tgacgacaca ttcaccgcaa ataaaaaaaa tgtcgccgta
     2401 ttgtgccggt tgatgctgga gaaaaaaatc aatctcagct ggtcatgttt tgcacgcgtg
     2461 gataccgtca caccggactt gctgcagttg atgaaaaaag cgggctgcca tcaaatcatg
     2521 tatggttttg aaaccacgga cgaagatatt ctaaaaaaca tcaacaagcg aatcaccgcg
     2581 gagcaatttc agaacgccat caaatggaca agagcggcaa agatgaacat ccgcggcgct
     2641 tttatgctcg gcaatccggg agaaacggaa gccagcatgc aaagaactat cgcttttgct
     2701 aaaaacgcac gaattcattt tgccgttttc aatatcacga ctccatatcc gggcaccgcc
     2761 atgtataaag aattcttacg caataattcc cttctacatt ataattggga tctgtataat
     2821 ctggcagaac ccgtgttgga cttagacacc gtatcggaca acgtcgtcag acaatattat
     2881 tataaaagct accgtgattt ctatctgcgg ccgatcttta tttttcggca tatccgcagg
     2941 atacgcactc tttccgaact gttgatgcac attaaagcag tactaaaaat aattcccctt
     3001 ctctatagaa atattttttc aaaataaatt tcaggttata aattaaaaat cttttctgca
     3061 aaaaaacagc agcgaacatt ccggaaaagt cttgctgata ttcccggaag ccaaagcagc
     3121 tatacagttc aaccataatt tcattgacaa aaaacggcat tctccctata tattcacaac
     3181 actcaactgg aaataacact ctatcacaaa gcatcatcag ccaggcaatg cgccatggga
     3241 cagataaccg atatgcccgg gaccgaatta gttttttaac cgctgtattg aaacatcaag
     3301 cacagccgtc ggaacattta tttcatgaaa ttatgtaaac ttataaaatt tccggatcat
     3361 tttaaggttg acatctcttg ctttcacgcg tttttgttaa gttgtctgat tgcactgatt
     3421 tccatcatga actctaatgg cggtattctg tatgaagaaa tgtatactcg tctgccctat
     3481 tatttgtcag atattccatt attgcagaag ttgttcgaca gtcaaatgct ggatgacggt
     3541 ttataccgtg cacgggaatt aagctattta tttgacttta tcgacgttaa atttgttgaa
     3601 ctctgcatag agcacggtta cccacatttt ttatctttaa cacattatct tctgtgtatc
     3661 gcaacaggtt gtataatttg gtccttttgc gtgaaaatat taaacctcag tcctttaaca
     3721 gggatcggct ggcttgtact gttttggact tctccaagcg tctatttcag cggatatttt
     3781 attaacagga caggaaaagt tatggtcatg tttctgacgt caattatatt ttatttcctg
     3841 tgtaaagcgg ttgttcttgc aaaggaagaa aatagctttc ctatctcaaa aattgattgg
     3901 tttctgtatt ccatatcaat gctgatcata acgtttctgg atgagcaggg agtattcctc
     3961 tgtatggtgt tttttacact cctgcttata tggcacctga ttatccgcca gaaggaattt
     4021 gccattatga ttttcgtttg tctggcaacc attattcttc acggattgta tcgatttttt
     4081 atcgctccgc acttcatatt tgctctcaac ggataccggc cggatttttc ttatcagata
     4141 ttgccgatga tactatatgt cgaaaattta gcgcagtatc tgcaggcggg attcttttta
     4201 tatcttgaca cattccgatt tttaatcggc aatcctcccc tgcccatcgg ctttattctc
     4261 ctcatattgc ttattttttc tccaatctat ttcatgtacg ctaaccgtca gaggtccgac
     4321 aactatcgaa agtgtttcgg tctggcgctg ggaggatttt taataacgaa tgtattgctg
     4381 gtcatcatga attcattcat gctgctaaaa catccgccac taatgtggcc ggaagccaga
     4441 agagtattct attggttgcc gacaacagtt attcttgtaa tgacactttc tgtgtggacc
     4501 cgtcttttcc ataagtccgc tgttttcaaa ttttgcatcg ttttttcaat ctgtcttgcc
     4561 gttgttggaa atattgtggc gcttcccaaa cacaaggcca ttatcatgag gcaagaaagt
     4621 tacgttcact ctaacattca atcaagctca gatttattaa aggcacttaa aaacctttac
     4681 gcactgacgg attccgatga tcctgtgatt gaaaaaaatc ctgtttttca attttttaag
     4741 cttaaaaaga aagactagcg gaataacctt atcatgcaca atggaagggg agaagcctgc
     4801 gatacgagac aatatcccca gagataagca gacatggaaa gtttgatctg aaagttccga
     4861 tgtgaaaatg acgtcttttc gtcaagagga ttgttgatta aaaaaggttt tagataaaag
     4921 aagaatttat caattcttct taaacatgat caatagtctt ttcagaacaa aaccatggct
     4981 atcggcagca atttattttg ttctccaaca aaaatcggat catcgtaaaa tatttattca
     5041 ttatttccat tgaatctaat ctttctcaaa acttcatgag caacggatgc atgaactgat
     5101 tttgaaggat gcccgtcatt tttatttatt attctttctt gcaggggaat atttttaacc
     5161 aatcgagaga cgttgatagt agttatttta tgaacttcaa aataattcac gatatcattg
     5221 acatatatag aattgctgat ttcaatatcc tcgaggacag gaaacactac cgcgataagt
     5281 tgtatatgat tttctcgagc gtaattgatg aataatttaa gctgctcctg gtgctttaat
     5341 aacatattat tatcccgata tgtttgttgc attaaagaaa gagctgaaga cgtaagttct
     5401 ttattgggga aaagaaaata catgtaatta agaaaataag agcctcttac cgcaggcttt
     5461 aggaatgcat tcacatttac gaaaggttta aaaccttcaa acgtttttaa tttttccgcc
     5521 gcaacatgcg caatatcatc gccgaagtat tggagcacta tgatttcggg cctaatcttt
     5581 gtcatataaa tgacttgttg catcaaatga aactcatcag atgaatccgc tcccgggtgt
     5641 cctatattta ttacagtata tttattttct tttttattta attctttgcc aacaatattg
     5701 gaaaatcttt catctacaga tttcaatcca ctgcccgccg taaatgaatc gcctacaaat
     5761 aaaattacgt tatgattgta attaggctcg ttgtctcgaa aacctaaaga gttgatcggt
     5821 ttataatatt ttgcatacca caatttggac gctaaagtat aatcaaagaa atgcgttcgt
     5881 ggaacaaaca tgaagacagt ttccaggatc atgaaaataa taaaaaatga aaataaagtt
     5941 gtggcaaaat tcgacaatgc atgattgatc tttgcatcga tgataataat tttgaacaat
     6001 ttgaaacttt caaggactat aaaaaacagc agagctaatc tgaagtaaac agcaataccg
     6061 gtagaataaa ccacaggcaa aacaaaagta actatcaaca gggcaattat caacaaactt
     6121 ttaaaaaata tttttttcat agtaacaaat tagactgccc ccccacccgg attgaacaaa
     6181 atatgttaga ctagttggca ggaaccagat tccctttatg ggaaagaaag gaacttgcca
     6241 tgaaacactg cagatcattt gctgttgaac tcaagcgcca gctcgttgaa gaacttttaa
     6301 gcggtatgag tacaccagcg caactgattc gccgtcatga gaaatcatcc ggccttcttt
     6361 atcactggaa agagcaatat gccaaaggac ggtttaacaa tgacccctcg aagtaagccg
     6421 cccaagggtg ttattcatca ttcggatcgg agcgcccagt atgcctccca tgattccgta
     6481 gaagcgctct tgcagcatgg ctttccgatc agcattgcgg gcaaagggaa tccctacgat
     6541 aatgctacca cggaaagctt tttcaaaacg ctcaaggtcg aaaaagttta cctttgggaa
     6601 taccgaaccc tggaagatgt tcagattcgg cttccctttt tcatccaaga attttatcac
     6661 cgcaaacgtc tgcattcctc tcctggatac aaaccgccgg tggaatctaa agaacttttt
     6721 ttataaaaac caaaaacccg cgccgattgc tctaacccga tctatctaac cgcaggggct
     6781 caatccagag actcttccaa cctatcttga gcggcatgca atccggaagg ataacctcat
     6841 gcccttgccg tctggcctcc gatccaagcc aggcaagcgc ctgaagaagc agataatcat
     6901 cctgctcatt catgtaatgc catatatatt tcggtggccg aacaaataat aatcgcatta
     6961 cttcctgtct ttgaacagtt gcttcaatat caacatcgct ccattaaaca tcattttcaa
     7021 accgattgaa cgtatcacaa aaagatgccg gaatatcatc tttggacgca gataaaactt
     7081 tacgtttgcc atccgatgca gttgcaagag ctcttttccc gtcatgccct caggaatata
     7141 gggaagcgag tccggatcgg gattgaaggt tgtaaagctt tcccattccg gttgtgatat
     7201 tttcccctgc ttcaccaaat catcatagag ttccgaaccg ggaagcggta tcgttatcgc
     7261 gaactttgca aaatcaatgt ccagcgatag cgcaaaatct atcgtctgtt ttgccgtttc
     7321 ttttgtctcg cccggaagcc cgagcataaa aaaagcggag atttcgagtc ccgcagacct
     7381 tgcagcaacg atcccccgct tcacattatc cgttgtatac cctttaccca caccggctaa
     7441 aacttcatta actccgcttt caatgccgaa caagattcgc ttacatcgcg ccagtttcat
     7501 ctcccggcac atctcctcat cgacaatgtc cactcgtgtc tcgcatgtcc accaccattt
     7561 ttcaggcatg ttttttgccc tgatggcccg acatgttgca atcgcgtgtt ttttatcaag
     7621 tggaaaaatc ggatcaacaa atccgatatg tcgggcaccg aattccttca cgagccattc
     7681 gatctcatcg gcaatcgatt ccggactgcg ttttctgacc agattgccca tataacccat
     7741 cgcgcagaac ttacaccgga acggacagcc cctgcttccc gaaatcgcca aacagggctt
     7801 ggcgactgta acaaacggca aaaaagaata atctctccag ggaaacagtt cccaagcggg
     7861 gcggggaaga gcgtctaaat cttctatcgg atcggatatc cctgtaaaaa cgattccttc
     7921 ttcgccctta taggatatcc cctgaatttt tgaaatatcg ctcttgttta aaaatgcatc
     7981 gatcaacctt gggaaaataa tctccccctc gccatgcacg accgcatcgc atgctccccg
     8041 attgataaga ttttcagcaa atagcgacgc atgtaaattt ccaaaaacaa ctttgacaga
     8101 cggcagcctt gttttaatgt aatgtcctaa agcttctgtt gaatttgcgg ttggcgtcaa
     8161 catacttatt ccgacgatat cagcgccaaa agatttgaca gattcaacaa tgtcttcaaa
     8221 aggtttcttt tccacaaaat tgtcgagcat gaatacttca tgaccggctt tttggcagct
     8281 cgaagcgatg tacgccaaac cgatacacgg catgggctcc attaacttgt gatactctcc
     8341 aaaagcggac agaatgtcca tagaagggtt tattaaaaag actttagcca tcttcacttt
     8401 tccccaacag ccataaaaat cctggcccgt tcggcaatca cggaatgatt ccggcaaaga
     8461 tcctcggttc gaataatcat atctacatat gttggatcat tcaggcgcga gtcgtcaatg
     8521 gcttgccaga atgccccatc cccaaaatcg tgcgaactgt ctatggtaag cttcttcaca
     8581 cccgcattcc ggacgagttc tttaaaatct tttcggttac aataattgat caaacggtcc
     8641 ttttcaagca gaagcggatc tctcgaaaga acgttcagaa gcctttctgg cccttcaacg
     8701 ccctgcgagc ataatgcacc caggcatcct tcaacggata aaaatatttt tgcgtttggt
     8761 ttcgcaacgc gcatcaaacg cttaagggat tttagaggat cggccgtgta acaaatgagt
     8821 cccatcgcta tcacgacgtc aaaagtattt tcccccacgt tgtccaaaaa cgaaatatcc
     8881 gcccagtgca tctcaacatt atgaagcccg cgctcatcca gatgacgctg acatgctttc
     8941 aacgacggca atgaagggtc aaatgcaaca accttcttga atcgttcagc caaaagggtg
     9001 gtaaatcttc caacacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaga
     9121 ggaactattc cctcagcctg ccaagccttt tttgtcaata tcaatgctcc ttatccgttt
     9181 ccaagagctc gttttgtcac actttacaac aataaatcat aggagatgaa ataatcaatg
     9241 tgcaaaggca attttataag aaaggtttta acaatacttg gacgtcttgc gcttttccct
     9301 gttaaatcaa caggagacac agcgccctcc gttctttttt aaaaaaaaga tttctcccca
     9361 cctggagacg taaaacaagg gcacaactac attaatgggg tttcggcctt ggcttgggag
     9421 agggtctcca cacctgtatt ttccccctcc ttatggtttt atttctatat aaaaataaag
     9481 gccgagtcaa tacatattat gagaaacatt agccattttc atcatcatct gcaaccaaaa
     9541 gcaaaactta aaaaatcaca gaggggaaat gacataatat caagcttttt ttacacatag
     9601 aaactattcc gggcacctat cattttcttc aaacaagcgc tcaatgtatg agtgttgtat
     9661 tgatttaaaa accaaaagga attccttact attgacttat attggttttt tgctataaaa
     9721 acaaaaacta tgaattggga ttttcataat attcttttct acccgccgat aaccggaacc
     9781 actattttat cggttgatct gccgccgcat ttcagagtaa ttgcatactt gattgcaggt
     9841 attgtcttac tcctgggaac atcatttttt tttgcaaaaa aatattctct gcttaacgct
     9901 ttcagaaaag ctatcattat cgcgtttttc tcctctgcgc ttttctacgc aatttatacg
     9961 gacatagggt ggagtacatg gctgaaaacc gatattaaga agtactgggg tctctcaaca
    10021 gaagagaagt ctattaaaat gtataacggc ttgtatgtat tctctatgga agcaaaaaag
    10081 ctgatccatg atgactacat aatatattct tcctattttc aagaaccgag actttttcaa
    10141 tattatctac tgccgctgaa caaacgttcg gataatcact accggcttga aaaggcaccg
    10201 tacattgtcg tacttaaaga caccgaatcc caatatgatc ttgctgaacg cgttttcacg
    10261 cgaggtgaaa tcaccatcaa aaacgtagag ttgatttcaa tgttcgcaca tgacgcttat
    10321 atactaaaaa gattatgatg ctatccacag ccatcgccat atttttacca accttacttg
    10381 gttttctcat tatcaccata atcctcagaa acgataaaga aactttcttt ggcgaacgca
    10441 tcggcctttc tttccctctt ggcgccggca ttttgacact gcaaatattt ttgttgggac
    10501 tcatgcggat tccactgaca ttgtttaatt ccttaatgcc tgtgtttttc gaactgatgt
    10561 tactttcatt ctggatatgg aaaaataaga ttgttttaat tccctcgatt tcaaacccag
    10621 gtttgagttt atttctggag atcaagtccc ctcgaaacca tgggttaaag aaatgtttat
    10681 ttgctctgct tatcatatgg atcattgcaa aacttgtttc tgtatttatc ttaacaggac
    10741 tcaggccgat ttttgcctgg gatgcatggg caaactggtc tgcgggagca aaaatcttct
    10801 tctactcaaa gagcttgctg cttgatgctc ctgcactgga attctttggc ggtaatgcag
    10861 tggatagaat cctgtactat ccactgcaca acaccttact gcagttatgg atgagccttt
    10921 ggatgggaaa gtttgatgat gtatttgtga agttcttcag cccggtttat tgtctcagta
    10981 tgattatctg tttatattac atcgccattc gtgaaatcgg caagctctat gcccttgcac
    11041 tgctcgccat ctttctgagt tcaccgcttt tatcctatca ttccatcgag atgtatagcg
    11101 atcaaatgct cggggtttat ttgttgttcg catccttatc ttttttgaaa gccataaggc
    11161 aaaacctctc tttctgtata ctggccggag catacgcaac gatagctgtt tttactaaaa
    11221 acgaggcctt gttctttgtt ttgcccttcc ttttatcggc catcgtatat tttaggcatg
    11281 atttaaataa aagccgcgga aaatatgtca acctgatttc aattttagca ccttttttag
    11341 cactacttcc ctggttttta tttaaagtcc attatgggct ggggtttttg ggacagttgc
    11401 gatggttgcg atggtcacat gaattatatt tcgaatatta tgcgcattca cctgatacct
    11461 cattgtggtc tttttttcag ccggacatga ttttagttta tatatacaaa atgatatcgc
    11521 tggacaactt taatgtcatt ttccttttct ttccgttaat tattctggca catggaaaaa
    11581 tatcaaaaga gcttttgcat cttcttgttc ccattgcagg ttatatgctt ttctttcttt
    11641 ttctctacat ctttaccgca tattatatgg gacaatcgat gtgtctcacc cgcaacatac
    11701 tgacatttta cccgctcttc ttttttctaa ccatattgct tcttaagaag gatgacactg
    11761 tactttctta tccggcaccc ataacaagat aataccgtgt cgtcattgtg attcacggga
    11821 aattctttcg tatatcaaaa tgcggcatta tcagatttac tcgataattt atttttactg
    11881 aggaataacc ctatattaac cgcgaaagat cccgcaatct gaatcaaaaa aggataagac
    11941 ggcattagat ggcgattcat atcgtccaga ccggccagcc atatggaaaa aaattgcccc
    12001 agcacgaaca cgatgcaaaa cgactgagcc caggcaattt ttttgtattt tacagcggca
    12061 accatgatca ataccgattg cagggcaagc agcagaaaca gatttccgta ggcggccacg
    12121 ataatatcaa aagctctttg ggccatgtat ttgacataaa caactgtgca ccgggcatgg
    12181 ctgatgaacc gtatgatcct ttcgggagaa taattaaccc tgtcaggaaa gaccgaacag
    12241 aaaatcatat cctccgtcgg cggcaggtct ttcggaaagt tccgataaca gcgcttgtag
    12301 ttatcgataa aatgattatt gatcgaaaaa ggagcggcat aataattgat atgtcgcgtt
    12361 ctgtcgatga cggccatgaa ttcctcatct ttcccgtatt cgtaagcccc gcttatactt
    12421 aaatgagcaa tcgtattgtt cagcgcgatg ttactcaaaa caaactggcc gttgaacttt
    12481 ttattcattc cgcaatatcc gagcacaccc gcagccgtga agcacaatcc cagcaatccc
    12541 caatagagga tttttctttc gtcgcgcagt agaaaaaaac ggcacaacag aaaaagaagc
    12601 atgaccgcta tcaggatcaa ataggtcggc ttcagcatga tcaggaaaaa cgggaaaatg
    12661 ccgatcgcca gcgcggcgga ccgtttcggt ttttcaagaa accggacaag cagggacagc
    12721 ataaagaccg atccggcaaa gcacaggctc tccggattga gatggacatt ctgaatcagg
    12781 atgggatgcc agcagccgta aaagagcgtt gcaatcatgc ccacataaac attgccgatg
    12841 acgcgtttgg aaacgaaata gaacgggatg atagacaaga acgacagggc ctgctgaaaa
    12901 aaaatgagat tgcggaccag atgatctccg gaaacatatt ggaagaattt gatcacatac
    12961 ggataaagcg gcgtgcggta caggtcaacg cctcccctga acaaatccac ggcgtagaaa
    13021 tacgaaacgc tgtccgaatt atactgcacc attccgggat tcaggaaccc gaagtaataa
    13081 attctataca gaagacaaac cacaagaatc acgcctaaaa aggcgtaatc aggaatgctg
    13141 gctattttcc ctttcaaccg gttattctct gtcatcacgt tgtgttccac gaagataatc
    13201 gaatatccat ttttttatta ccgattgcaa taaaaaaatc tcttaatcat ccattatcca
    13261 tccctcgtcg ctgcgctcct gggatagttc cgtcagcgct ttaatctgcg cttcgcttga
    13321 tttcagcgga cgtcactacc atttcccatt gacatcaatc agcaagttcc ctatgctctt
    13381 ctcaaaaaaa cggggagctc aaaatggcac gcagactcaa tggtaaaaat gcgatcatca
    13441 ccggagcagc caatggaatc ggtcgcgcct ccgcagtgct tttcgcccgt gaaggggcca
    13501 accttgttct tgccgatatc agcgaaaccg ggcttcggca ggttgaggac gaaatcaaag
    13561 ggatgggagg aaacgtcgtt atccgcaaaa ccgacgttgc atcggaagaa gaaatccaag
    13621 cgctgatcaa tctggccctg gaaaaattca atacgattga catcctggtc aacaacgccg
    13681 gcatcggcgg cgggctggac gcgcttgaag atcagaagcc cgaggaatgg catctcgttt
    13741 atgatatcaa tgtcatgggg ccggtgtatg ccaccaagca catcatcgct catatgaaag
    13801 aacgtcgccg gggatcgatc atcaatatcg catcagtggc cggtctgctc tccggcgccg
    13861 gcggcaatgc ctacagcgcc agcaaggccg ctttaatcaa cttcacgaaa acgtccgctt
    13921 gcgaagtcgg ggccttcaac gtccgcatca acgccgtctg cccgggactc attgaaaccg
    13981 gcatgaccca gccctttttc gattgggccc gcagcaccgg caaggaaaat cagctcggca
    14041 aatactgcga gttgaaacgg gccgcctctc cggaagaagt tgcctgggcg atccttttcc
    14101 tggccagcga tgaagccggt tacatcaccg gccaggccat tgccgtcgac ggcggcatca
    14161 cggcctcgat gagcttgccc ggccggaaaa tataaaataa ttcagattct gaaaacttta
    14221 cttcagatcg cgtatttttt caggtcctgc atcctcatct ttgtacaaaa ttgtatattt
    14281 tcacgaaaaa attcaaataa attcaaataa atcttgactt tatt
//
LOCUS       DAONQZ010000055        14202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4752_length_14202_cov_16.025023, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000055 DAONQZ010000000
VERSION     DAONQZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_14202_cov_16.025023"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..962)
                     /locus_tag="PLR13_08715"
     CDS             complement(192..962)
                     /locus_tag="PLR13_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQC19460.1"
                     /translation="MTAQYLLLDVEENVATATINRPDKGNSLSPQVLEDIEHTFTELS
                     RRQDVHVVVLTGGERYFSAGFDLTYIRTLQKAHNEAYTALFHRAYRAVMFCEQPVICA
                     VGGPAIAGGFDLTMMCDIRYASERARFGQREIVLSLNPTIDPLWRIVGLSNAMESALT
                     GRIWDAQEALRMGYISRIFPEGQLLKEVGAIAREMATYDRGCLKETKQLAHLLLNSSL
                     DQAMRQQEWLFRTYIGSEDNHQRIDDLQTKLASMRRNK"
     gene            complement(991..3414)
                     /gene="lon"
                     /locus_tag="PLR13_08720"
     CDS             complement(991..3414)
                     /gene="lon"
                     /locus_tag="PLR13_08720"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQC19461.1"
                     /translation="MTEQTVPQQNKNTFNIPEMIPILPLHNVLVFPKTMIPLEVTGSA
                     SVLVDEAMTRDRLIGLIMSRKEPDKPNDYKKDDLHPVGTCAVIMKMAKTMDNHTQLLL
                     QGVSRFSIEEIIEGKPYQQARIKLLEEKDAKDLETEALMSNLLSLFDRILKLSPFLPP
                     EFGPMAKSITEAGTLADLIASIINAPVEEKQKILNIADIKKRLKELTLMVNHQMDVLE
                     LGNKIQTKVKDDIDKSQREYYLRQQLKAIRQELGETDENAVETEEYRKKIEEKNLPEE
                     AKKEALRELERLSRMHPSSAEYTVSSTYLDWMTTLPWNESTNDNLDIGKARRVLDEDH
                     YGLTKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGQSIARALERKFVRISL
                     GGVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKVGSDFRGDPSSAL
                     LEVLDPQQNNTFSDHYLDVPFDLSGVMFIATANMLDTIPPALLDRLEVIELNGYTQEE
                     KVKIAQRYLIPRQLKENGLNSSQFKLTHKALNAIITGYTREAGVRNLEREIANACRGV
                     AARIAEGAVKSGTLEEKDIPAYLGPVRVMTDLDARIAKPGIAIGLAWTPVGGDMLFIE
                     ATAMKGKKGLTLTGQLGDVMKESAAAALSFIRTNAYALNIKPEFFDDTDIHIHVPAGA
                     IPKDGPSAGVTMFAALTSLLTNRKVKKQLAMTGEITLRGAVLPVGGIKEKVLAAYRAG
                     IKTILLPEWNRKDLEDIPGNVRKSISFHFVKDMMDIIKLALEPVPQKKMKRASARKSK
                     PAVPSKTKK"
     gene            complement(3454..3894)
                     /locus_tag="PLR13_08725"
     CDS             complement(3454..3894)
                     /locus_tag="PLR13_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQC19462.1"
                     /translation="MTYIKINFGSNFEDEFQKVVDEVFHLVRPAFHNYECIWRPNVDV
                     YESTDEIIVLADMAGLNKEELHIEVNRKQIKIAGVRKAVQHLQNAHYCQAEIPHGYFE
                     RIISLPAPVESQSAKASYADGILMVRINKLPVSKSHRISIISEK"
     gene            complement(4134..5141)
                     /locus_tag="PLR13_08730"
     CDS             complement(4134..5141)
                     /locus_tag="PLR13_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC19463.1"
                     /translation="MTKKIIMLVLLMAVLFHIAWYAVSRSVQHEKIPDDATAQAAFDK
                     LLKDEEISIDRHAFTEMSIEANGIRIHLDVFSKGSNGPTIVFIPGTSVYSKFYIESMY
                     KLYLQGFNVVGFDPRGHGMSSGLRGDYTVNEIVEDTLAVVKFARKRFSGPVAVVGSSQ
                     GGIAAFYAAARDDSLAAAVCHNLADLNGKDNLILSQIRPPVWMAPIAGFLADLYKRFS
                     VPVFLYLDLTQEKSRSGTDAKTLIKNNPVTVKWITLRALGSLMKTDLAKPVEQIKVPV
                     MLIHAERDSIFPQAYVEDIFNRLTCPKKYLFFKDTEHLVMTNNVDDVVPPIAAWLRDM
                     M"
     gene            complement(5138..5989)
                     /locus_tag="PLR13_08735"
     CDS             complement(5138..5989)
                     /locus_tag="PLR13_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQC19464.1"
                     /translation="MRIEKIEDKCSECMLCVHDCVMGVWRVVDGQSRPVAVDLCNGCS
                     HCIAICPCDAIVHDGLRKEELAGINRKNLNPDVYRDIVMSRRSIRQFKNEPVPRELIE
                     KVLDLVRYAPTARNHQDVGYIVVTDKPLIEKTAADLFGWGNRLYNMTTSGIGRRIINL
                     TGLNKNPYYNLMAYVQMQNAEKGRDYILHHAPVVILIHAAKRKMFVCDDSNIAAATII
                     NYAHTLGLGTCFVGMMTLMLRFSGRLRKTFGVPDNRRVYATLVMGYPAYRYKNTVSRK
                     KAEVQWR"
     gene            complement(6189..6398)
                     /locus_tag="PLR13_08740"
     CDS             complement(6189..6398)
                     /locus_tag="PLR13_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQC19465.1"
                     /translation="MSSIYKVIEVIGGSEKSWEEAAKKAVEMTAKSLKEIRIAEVKEL
                     DMRVEDGKVVEYRVKLRVSFKYLPD"
     gene            6801..8609
                     /locus_tag="PLR13_08745"
     CDS             6801..8609
                     /locus_tag="PLR13_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQC19466.1"
                     /translation="MHKFTLDKPDNLVDWWGESVTRFADRPLFGTKNKEGVYKWATYK
                     EIGNRIDNLRAGLTQLGIGKDDVVGIIANNRPEWAVIGFATWGCLARYVPMYEAELVQ
                     VWKYIINDSGAKVLFVSNPAIYEKIKDFQKDIPTLKHIFIIDSDGDNSMASLEKKGAE
                     KPVAPKSPKAEDVAELIYTSGTTGNPKGVLLMHMNFTSNSHAGLKMYPELYENEVVSL
                     TILPWAHVFGQTAELFAIIRLGGRMGLIESTKTIINDIVQIKPTFIIAVPTVFNRIYD
                     GLWNKMNKDGGLARALFVMGVEAAKKKRILAEKGQSDLMTNFKVAVADKIVFKKIRER
                     MGGRMLGSMTGSAAMNVEISKFFFDIGIPIYDCYGLTETSPGITMNGSQAYRIGSVGR
                     PIDKVKVVIDSSVVEEGATDGEIIAYGPNVMKGYHNRPEDTKAALTPDGGFRTGDRGR
                     LDKDGYLFITGRIKEQYKLENGKFCFPVSLEENICLASFVQQAVVYGLNRPYNVCIVV
                     PDFDVLLDYAKEKGLPTDIKTLVEREDIIHMISEAVTGQLKGKFGGYEIPKKFIILPE
                     AFSLDNGMLTQTMKLKRKVILDKLNDRIEALYKEDK"
     gene            complement(8700..9518)
                     /locus_tag="PLR13_08750"
     CDS             complement(8700..9518)
                     /locus_tag="PLR13_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19467.1"
                     /translation="MFKISEQESILLFYMGRSPLGILLYPVYAFLVGDVLIDTGTNRA
                     GHEFLEALKGRRIAGIINTHHHEDHIGNNADVQQHFGINIYAHPLTLPYLENPRLNDL
                     HLYQRIVWDWPRPSKGEAVGASVKAGPYHLEVIHSPGHAEDHICLYEPDKKWLFTGDL
                     FCGTNFVYLRQDENYLQILDTLKKLSALNIETIFCNLKGVVKDGKIALAKKISTMERL
                     RDDVFRLRDRGLSPQRIRQKVLGREDARHFFTRGHYSKQNTVDSILSGKRPANR"
     gene            complement(9553..12171)
                     /locus_tag="PLR13_08755"
     CDS             complement(9553..12171)
                     /locus_tag="PLR13_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HQC19468.1"
                     /translation="MFFLITFFAAPAYTAEETARSARQVPAESGKSSAFTPKETTPIV
                     VSVIINTQSRGDIFAELDDRGNLYIRPDDAKALKLQYSEDRIVILRGDEPHVPLNALL
                     DVTSTFDEKKLTVTVIGKTTESTRTAADLFSLRAETKNIYYPRETSAFVNYGVNYAFT
                     GQGGFQTLSVTNKLGFRTGDMFFTSDSVFTKTESDYDLVRLQSSATYERRKDLQWLVM
                     GDQYANSGELGSTVNMGGIGFSKVYRLDPYFITQPVMDLQGTVIYPTQAEIYLNGVLI
                     GKQDIAPGSFDLKNIYSQTGSHNIEVVLKDPFGNVQRISYMAYFSAQMLRKGLHEYSY
                     NAGFLREQYGSESNEYGDLAFSAFHRYGVTNKVNIGASAEGSDGVYNGGISTTFAVPL
                     AGQFTVQLSGSSSDEIDKRGDAVSLQHNLQLGNFSTNLLLRKYSRYYATIGNPLPTED
                     NTRHEIRAGMGFSLNPLGSFSVNYAQTENFKGMDTQVISLNYSRTLYRSISLFATGSA
                     TRATGEDTNYSGFIGLNFALMDNVRGSAQVNFGSDDVNSETLQVQKDIPVGEGLGYRA
                     SVNRSETAANTVYTTNPYVQYNARYGIYSFDAQIQDSKNGISTESYNLSAAGSMVWAG
                     EYLGLSRPVSDSFGVVLFNKNVPGASVLNNGQEIGKVGSLGTMVVPTMSSYGQNKITL
                     DTKNIPIDFTISDVNKTLSPSLWSGSCIYFDAQQLRAVTGTIFARKEGKKIPLEYVEI
                     AMKIGEKELLSPTGKGGEFYLENSFGEDDAAGSSTVVDKQSCQAISRMLKAGGSTISP
                     GTYKASVEYEGGECGFAVTFPETEEVITELGEIECVATSNSPRRKDTSLRRRSLYYAD
                     YAGKAAAISDQILVKQ"
     gene            complement(12245..12982)
                     /locus_tag="PLR13_08760"
     CDS             complement(12245..12982)
                     /locus_tag="PLR13_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HQC19469.1"
                     /translation="MFVIIYSTHALAADFQVQPTTMDLGGKVKSGVFSVINNSNEKID
                     FQVSVKAWNQDENSKDAYTDTNDIVFFPKVMSIDPNSQRAVRIGLKTPPGAREKTYRL
                     FVQEIPTQKKTQDIDVNQKVKAGVTIAFRFSMPIFVKPLKPQEIYAIDKVEMAAGKAR
                     AVVKNTGNVHVKLRSVTFSGKAADGKELYSKEVAGWYILNGMSSSYEAEIPKDVCGQL
                     AKIDINVRTEDKDISGILNVQKNMCPE"
     gene            complement(13168..>14202)
                     /locus_tag="PLR13_08765"
     CDS             complement(13168..>14202)
                     /locus_tag="PLR13_08765"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HQC19470.1"
                     /translation="VHAAINCPGTLPEGTRGVAYSYTFTATNGNGGSPWWRIVMGSLP
                     PGLSLINQSLNSVTLAGTPTTAGTYWFAINHRQPGMDPICFSTIVINAAGCVLNGTDT
                     GGISFGNLDPSGYPAPVNNIITQQVNFQCSAGLAYSIAVTNPVGQLQLTGANTVPFTL
                     GLHANGTGAGAVDIPLLTMNSAITSYAGFVEGVSSFSNPITVEINWAGGSLSAYVNVS
                     DVTVIPACGTPTNGTMNFDIDPSLNTTITASTTANGTSPTVKCTNASQPNVTCETSGG
                     STAGQLKKGTDNINYNVTTCGVSGKITGNGFGAAPASEIPVGISVVPSAYQSAPPGDY
                     SDNVTVTIAY"
BASE COUNT         3549 a         3623 c         3295 g         3735 t
ORIGIN      
        1 taaagaggtt ttgaatccaa acaagcactc cctctgggta aataaatatt ggcactttct
       61 tgatttacgc gggagtatat aaaaaagctc gtcgtctgtc aacgaaatac agacgcttgg
      121 tgattatatc ctgattaagc ataaaaggaa tctattcgaa aaataattca accagaaccg
      181 gaagaaaggt cttatttgtt ccttcgcatg gacgccagtt tggtctgcag atcatcgatg
      241 cgttgatgat tatcttcgga accgatgtaa gtacggaaca accattcctg ttgacgcatg
      301 gcctgatcca gactggaatt cagaagcaga tgcgccagtt gcttggtttc cttgaggcag
      361 ccgcgatcat acgttgccat ctcacgggca atggcaccga cttctttcag aagctgccct
      421 tcggggaaaa tgcgtgagat gtaccccatg cgtaaagctt cctgtgcgtc ccaaatgcga
      481 cctgtcaggg cggattccat cgcattgctc aagcctacaa ttcgccagag cggatctatg
      541 gtgggattga gagaaagaac gatttcccgt tgtccgaatc tggcacgttc tgaagcgtaa
      601 cggatatcgc acatcatggt cagatcgaac ccgccggcaa tggccggccc gccgacagcg
      661 cagatcaccg gctgttcgca aaacatgacg gcgcgatagg cccggtgaaa cagggccgtg
      721 taggcttcgt tatgcgcttt ctgcaaggta cggatgtagg tcagatcaaa ccctgcagaa
      781 aaataacgtt cgccgccggt caagacgacg acatggacat cctggcggcg cgacagttct
      841 gtaaatgtgt gttctatgtc ttccaaaacc tgcggtgaaa gtgaattgcc tttatccggc
      901 ctgttgatgg ttgcggtcgc cacattttcc tcaacatcaa gtaacagata ctgcgctgtc
      961 ataattttta ccttcctaat tttttatttt ttattttttc gtcttcgacg gcactgcggg
     1021 ttttgatttt cttgccgaag ctcttttcat ttttttctgc gggaccggtt ccaaagccag
     1081 cttgataatg tccatcatat ctttaacgaa atggaaagat atgcttttac gtacgttgcc
     1141 cggaatatct tcaaggtctt tacgattcca ctcaggcaaa agaatcgttt tgatacccgc
     1201 ccggtaagca gccagaacct tttccttgat gccgcccacc ggcagtacgg caccgcgcag
     1261 tgtaatttct ccggtcatgg ccagttgctt tttcaccttg cgattggtca aaagagaggt
     1321 cagggccgca aacatagtca ccccggccga cggaccgtcc ttgggaatgg cgcctgccgg
     1381 aacatggata tgaatatccg tatcgtcaaa aaactccggt ttaatattca gggcataagc
     1441 attcgtccgg atgaagctca gcgctgccgc cgccgattcc ttcatcacat cgccaagctg
     1501 accggtcagc gtcagtcctt tcttgccttt cattgccgtg gcttcaataa aaagcatatc
     1561 gcccccgacc ggcgtccagg ccaggccgat cgcaatgccc ggtttagcaa tgcgggcgtc
     1621 aaggtcggtc ataacccgca ccggccccag ataggccggg atatcctttt cctcgagcgt
     1681 gccggatttc acggcgcctt cggcaatccg cgccgccacg ccgcgacagg cgttggcgat
     1741 ttctctctcc agattgcgca cgcccgcctc acgcgtgtat cccgtaatga tggcgttgag
     1801 tgctttgtga gtcaatttga attgcgacga attcagcccg ttttctttca actgccgggg
     1861 gatcaaatac cgttgggcga tcttcacttt ttcttcctgc gtataaccgt tcagctcaat
     1921 aacttccagc cggtccagca acgccggggg aatcgtatcc agcatattgg ctgtagcaat
     1981 aaacataacc ccggaaagat cgaacggcac atccagataa tgatccgaaa aagtattgtt
     2041 ctgctgtgga tcaagcacct ccagcaacgc cgaggacgga tcgcctcgaa aatcgctgcc
     2101 gaccttgtct atttcatcca gcatgaagac cggattgttc gattccgccc gtctcagtcc
     2161 ctgaatgatg cgaccgggca gagcaccgat ataagtgcgg cgatggccgc ggatttccgc
     2221 ttcgtcgcgc acaccgccca gcgaaatgcg cacgaacttg cgctccagcg ctctggcaat
     2281 tgactgtccg agcgatgtct tgcctgttcc cggaggtccg acgaaacaca gaatcggtcc
     2341 tttggaatcc ggcttgagtt tgcgcaccgc cagatattcg atgatgcgtt ttttgggttt
     2401 ggtcagaccg taatgatctt catcaagcac ccggcgagcc ttgccgatgt caagattatc
     2461 gtttgtcgat tcattccacg gcagcgtcgt catccagtcc agatacgtag aggaaacggt
     2521 gtattccgca gacgacggat gcatgcgtga caatctctcc aattcccgca aggcctcctt
     2581 tttcgcctct tcaggaagat ttttttcttc gatcttttta cggtattctt ccgtctccac
     2641 ggcattctca tcggtttctc cgagttcctg ccggatggcc ttcaactgct ggcgcagata
     2701 gtattcgcgc tggctcttat cgatgtcgtc cttgaccttc gtctgaatct tgttgccgag
     2761 ttccagaaca tccatctgat gattgaccat cagcgtaagt tctttcagac gttttttgat
     2821 gtcggcaata ttcagtatct tttgtttttc ctccaccggc gcattgataa tcgacgcgat
     2881 caagtcagcc agcgttccgg cttcagtaat ggatttggcc atcggaccga attccggcgg
     2941 cagaaagggt gaaagcttca ggatgcgatc aaacagggaa agcaggttgg acatcagtgc
     3001 ttccgtctcc agatctttgg catctttctc ttccagaagt ttaatgcgcg cctgctgata
     3061 cggttttcct tcaataattt cttctatcga aaaacggctg accccctgca gcagcagttg
     3121 tgtgtgatta tccatggttt tagccatctt catgatcacg gcacaggtcc ccaccggatg
     3181 caaatcatct ttcttatagt cgttgggttt gtccggttcc tttctggaca taatcagacc
     3241 aatcaggcga tcccgagtca tcgcttcatc caccagcacc gaagcgctgc ctgtcacttc
     3301 cagcggaatc atggttttgg gaaacacaag cacgttatgc aacggaagaa tggggatcat
     3361 ttccgggata ttaaaagtgt tcttattttg ttgcgggaca gtttgttccg tcatttttaa
     3421 cctccagtgt gattttccga aaagagattt tcgctatttt tccgaaatga tggaaatgcg
     3481 atgcgacttg ctcacgggca gcttgttgat tctgaccatt aaaattccgt cggcatagga
     3541 cgctttcgcc gactgggatt cgacgggagc aggcaaagaa atgattcttt caaaataacc
     3601 gtgcggaatt tcggcctgac agtaatgtgc gttttgtaaa tgctgaacgg ccttgcgcac
     3661 tccggctatc ttgatctgct ttcggttcac ctcgatgtgc agttcttcct tattgagtcc
     3721 ggccatgtcc gccagtacaa tgatttcatc ggtggattca tacacatcca cattgggacg
     3781 ccagatgcat tcatagttgt gaaaggccgg tctgaccaga tgaaatactt catccaccac
     3841 tttctggaac tcgtcttcaa aattactgcc gaaattgatt ttaatgtacg tcatgttgct
     3901 gcctcacaaa tctgtctcta tactggttaa aatataataa tgccctgacg acttgtaaat
     3961 accgcctcaa aaattcttcc gtttgacatg tttgtactcc agtcgactcc gctagggcaa
     4021 gaatatcgtt tgcctttttg aatcatacac agccgatcta taaaatcaaa tccggatttg
     4081 gacactttaa aaaccatgcc aaaattaaaa tcaacagaca cagagaggcc cttttacatc
     4141 atgtcccgga gccatgcagc aatcggcggc accacgtcat ccacgttgtt ggtcatcacc
     4201 agatgctcgg tgtctttaaa aaacagatat ttcttcggac aggtcaagcg gttgaaaatg
     4261 tcctcaacgt aagcctgcgg gaagatggag tcccgttcag catgaatcag catgacaggc
     4321 accttgattt gttccaccgg tttggccaga tccgttttca tcaacgaccc tagcgctctg
     4381 agcgtaatcc atttcacggt gaccggatta tttttgatca gcgttttggc atccgttccg
     4441 cttctggatt tttcctgcgt caaatccaga taaagaaaaa ccggaacgga aaaacgtttg
     4501 tacaggtccg ccagaaatcc ggcaataggc gccatccata ccgggggcct gatctgagaa
     4561 agaatcagat tgtccttccc gttcagatca gcgagattgt gacatacggc cgccgcaagg
     4621 gaatcatcgc gcgctgccgc gtagaaggcg gcaataccgc cctggctgct tccgactacg
     4681 gcgacagggc cgctgaatcg ttttcgtgca aatttgacaa ccgccagcgt atcctcaaca
     4741 atttcattga cggtataatc tccccggaga ccgctactca tcccgtgacc gcgcgggtca
     4801 aaaccgacta cattgaatcc ctgaagataa agtttataca tgctttcaat ataaaatttg
     4861 gagtacacgg acgtgccggg aataaaaaca atcgtcggcc cgttgctgcc tttcgaaaaa
     4921 acatccagat gaatccggat gccattggct tcgatggaca tttctgtaaa ggcatgacga
     4981 tcgatgctga tttcttcgtc ttttaaaagt ttatcaaacg ctgcctgagc ggttgcgtca
     5041 tcgggaattt tttcatgctg aaccgaacga ctgaccgcat accaggcgat atgaaacaaa
     5101 acggccatca acaggactaa cataataatt ttttttgtca tcgccactga acctccgctt
     5161 tttttctgga cactgtattt ttatagcgat acgccgggta acccatgacc agcgtcgcat
     5221 agaccctgcg gttatccgga acgccgaatg tttttctcag ccggccgctg aaacgcagca
     5281 tcagggtcat catgcccaca aaacaggtcc ccagaccgag cgtatgtgcg taattgatga
     5341 tggttgccgc ggcaatattg gaatcatcgc aaacgaacat ttttcttttc gccgcgtgaa
     5401 tcagtatcac gacaggggca tgatgcaaaa tgtaatccct gcctttttcg gcgttttgca
     5461 tttgtacgta cgccatcaga ttataataag ggtttttgtt caggccggtg agattgatta
     5521 tccgccggcc gatgcctgat gttgtcatgt tatacaaacg gtttccccag ccgaataaat
     5581 ccgccgccgt cttttcgatc agcggcttat ccgtcacgac aatgtagccg acatcctgat
     5641 gatttctcgc cgtaggcgca taccggacca gatcaagaac tttttcgatt aattcgcgtg
     5701 gcaccggttc gtttttgaat tgccggatgc ttctgcggct catgacgatg tcgcggtaga
     5761 catccggatt caggtttttt ctgttgatcc cggccagttc ttcttttctg agaccgtcgt
     5821 gaacaatggc gtcacagggg catatcgcga tacagtgact gcagccattg cagagatcaa
     5881 ccgcgaccgg tctggattga ccgtcgacta cacgccatac gcccatcacg cagtcatgga
     5941 cgcaaagcat gcattcactg catttatcct cgattttttc aatacgcatt caatgattcc
     6001 gcaaagacgt ctgctgtttt gatgagatta tttctgtttt gagttggata tataaagaag
     6061 cggcaggcat gtcaataaaa tattcgttgc cggattccgg acaggcggcg ttttgttttt
     6121 gcctgttgca gcggctgatt gatcagacac tgcaacaggc cggcttttct aaaatcaatt
     6181 ttggtatttt aatcaggcag atatttaaac gacaccctca gcttaacgcg gtattcaacg
     6241 actttcccgt cttcaaccct catatccagt tccttgactt ccgcaattct gatttccttc
     6301 aagcttttcg ctgtcatttc aaccgccttt ttagcggcct cttcccatga tttctcactg
     6361 cccccgatca cttcaataac tttataaatg ctgctcattt ttgccctcct ttcgcctgaa
     6421 tggtttaaaa atatttaaac atttcacggc ggctttccgg ccgtcaatcc tggataacgc
     6481 actgggcaat ccagtaagaa aaaacacaac gttcataccc gtttacagat cggaaaacac
     6541 aacccggcag caaaacaaaa gggaaatcac agaaagggat aacagattat gaatttttgt
     6601 taaattataa ttcattttta agaaacgtca aatgattatt ataagttctg aatattcaat
     6661 ccataattta atattcatgt ttcgattgaa tttttcataa aaatgactta gagtcacaaa
     6721 ccgtaaacga aaatatttat ttctgaagaa atccgcgcgc gacctcaacg tccgcgtgac
     6781 catacaacaa ggagtatttt atgcacaaat tcacattgga caagcccgac aatttggtcg
     6841 attggtgggg agaaagcgta accaggtttg ccgatagacc gctgttcggc accaaaaaca
     6901 aagaaggcgt ttataaatgg gcgacctaca aagaaatcgg caaccgcatt gacaaccttc
     6961 gagcgggact gacccagctg ggaatcggca aagatgatgt cgtcggcatc attgccaaca
     7021 accgcccgga atgggcggta atcgggtttg ccacctgggg atgcctggcc cgttacgtgc
     7081 ccatgtacga agcagaactg gttcaggtct ggaaatacat cattaacgac agcggggcaa
     7141 aagttctctt tgtttccaat cctgcaatat atgaaaagat aaaagatttt cagaaagata
     7201 tcccgacact caaacacatt ttcatcatcg actccgatgg tgacaattcc atggcctctc
     7261 tcgagaagaa aggcgctgaa aaaccggttg caccgaagag tccgaaagcg gaggacgtcg
     7321 ccgaactgat ctacacatcg gggacaaccg gcaatcccaa aggcgttttg ctgatgcata
     7381 tgaacttcac cagcaattca catgccggcc tgaaaatgta tcctgaactc tatgaaaatg
     7441 aagtcgtttc tctcaccatt ttgccgtggg cgcatgtttt cggccagacc gccgaattat
     7501 tcgccatcat ccgcctgggc ggcaggatgg gattgattga aagcacaaaa accatcatca
     7561 atgatattgt tcaaatcaag cctaccttta tcattgccgt ccccaccgtt ttcaacagaa
     7621 tttacgacgg tctgtggaat aaaatgaaca aggacggagg actggccaga gcgctctttg
     7681 tgatgggcgt cgaagccgcc aagaaaaaaa ggatcctagc ggaaaaaggc cagtccgatc
     7741 tgatgacaaa cttcaaagtg gccgttgcgg ataaaattgt cttcaagaaa atcagggagc
     7801 ggatgggggg tcgaatgctc ggatccatga ccggcagcgc ggccatgaac gttgaaatat
     7861 ccaagttctt cttcgatatc ggcattccca tttacgattg ctacggcctg acggaaacat
     7921 cacccggcat caccatgaat ggctctcagg cttatcggat cggcagtgtc ggcagaccca
     7981 tcgataaggt caaggtcgtt atcgactctt ccgtagttga agaaggggcg accgacggtg
     8041 agatcatcgc ttacggaccg aatgtcatga aaggctatca caaccggccg gaagacacca
     8101 aagccgctct cacaccggac ggcggtttcc gcaccggcga ccgcggacga ctggacaagg
     8161 acggatacct tttcatcacc ggccgcatca aggaacaata caaactggaa aacggaaagt
     8221 tctgcttccc cgtctcgctg gaagaaaaca tttgcctggc ttcttttgtc caacaggcgg
     8281 tcgtttacgg tctgaaccgt ccctacaatg tctgcattgt cgttcccgac ttcgacgtac
     8341 ttttggacta cgccaaagaa aaagggttac caacggatat caaaacgctg gtcgaacgag
     8401 aggatattat ccatatgatc agtgaagccg tgaccggcca actcaaaggt aaattcggcg
     8461 gttatgaaat tcccaagaaa tttatcattc ttccggaagc gttcagtctg gacaacggca
     8521 tgctgaccca gacgatgaaa ctcaagcgca aagttattct ggacaagttg aatgaccgga
     8581 ttgaagcgct ttacaaagaa gacaaataat ccgataatgt ttccagacct cccgtaaaaa
     8641 gagaaaggcg tgttttccgg acacgccttt ctctttggtt tgctgatccg tctctccgtt
     8701 tatcgattgg ccggcctctt tccggaaaga atgctgtcaa ccgtgttctg tttggaataa
     8761 tgcccgcgcg taaaaaaatg ccttgcatct tcccggccca gaactttttg ccggatgcgt
     8821 tgcggtgaca gtcccctgtc gcgcagtctg aaaacatcat cccgcaaacg ctccatggtt
     8881 gaaatctttt ttgccagggc tattttgccg tctttcacaa cgcccttgag gttgcaaaaa
     8941 atagtttcta tgttcagggc actgagtttt ttcagtgtgt ccagtatctg caagtaattt
     9001 tcgtcctgcc ttaaatatac gaaatttgtt ccgcaaaaaa gatcgcccgt gaaaagccat
     9061 tttttatccg gctcatataa acatatgtga tcctcggcgt gccccggcga atgaatgact
     9121 tccagatgat aaggtcccgc ctttacagag gcgccgacag cctcgccctt tgacggtctg
     9181 ggccagtccc acacgatccg ctgatacaga tgcaaatcat tcaaacgggg attttccaaa
     9241 tacggcagcg tcagcggatg cgcgtatata ttgatcccga agtgttgctg gacatcggca
     9301 ttgttgccga tgtgatcttc atgatgatgg gtgttgatga tcccggcgat ccttctgccc
     9361 ttcaacgctt caaggaattc atgccccgca cgattggtac cggtatcaat cagcacatcg
     9421 ccgactaaaa aagcataaac gggatacaga aggattccca ggggcgaccg ccccatatag
     9481 aaaagaagga tcgattcctg ttcgctgatc ttaaacaatt ttccccaccg cttagacaga
     9541 cgctacgttc tgttattgct tgactaaaat ctgatcagaa atcgcagcgg ccttgcctgc
     9601 atagtctgca taatagagac tcctccgacg aagcgatgta tccttgcgtc ggggcgaatt
     9661 ggatgttgca acgcactcga tttctcccag ttcagtgatg acctcttccg tttcggggaa
     9721 ggttacggca aacccgcatt cgccaccttc atattcaaca ctggccttgt aggttccggg
     9781 ggaaatcgtg ctgcctccgg cttttagcat ccgtgaaatg gcctggcaac tttgcttatc
     9841 cacgacggtg gaggatccgg cagcatcatc ttcgccaaaa gaattctcca gatagaattc
     9901 tccgcctttc ccggtaggag acaataattc tttttcaccg atcttcatgg cgatctcgac
     9961 atattccaac ggaattttct ttccttcttt tctcgcaaag atcgtccccg taaccgcgcg
    10021 caactgctgg gcatcgaaat aaatacagga accgctccac aaagaaggag acagggtctt
    10081 gttgacatcc gatattgtaa aatcaatcgg aatatttttc gtgtcgagag taatcttatt
    10141 ctgaccgtag gaagacatcg tggggacaac cattgtgccg agagaaccca ctttcccgat
    10201 ttcctgaccg ttgttgagca ctgacgcacc cggaacgttt ttattgaaaa gcaccacgcc
    10261 gaaactgtcg ctgaccggcc gcgaaaggcc cagatattcc ccggcccaaa ccatcgaccc
    10321 cgccgccgac aaattatacg attccgttga gattccgttt ttagaatcct gaatctgggc
    10381 atcaaaggaa taaatgccgt agcgggcgtt atactgaacg tacgggttgg tcgtatacac
    10441 ggtattggcg gctgtttcgg aacgattaac ggaagcgcgg tatcccagcc cttctccaac
    10501 cggaatgtct ttttgcactt gcagcgtttc ggagtttaca tcgtcgcttc cgaaattcac
    10561 ctgtgccgaa ccgcgaacgt tatccattaa agcaaagttc aaaccgataa atccgctgta
    10621 gttcgtatct tcccctgttg cgcgtgtcgc cgaaccggtc gcaaaaagac tgatagatct
    10681 atacagtgtc cgggagtaat tcaatgatat gacttgcgta tccatccctt taaaattttc
    10741 cgtctgagcg taattaacgg agaaacttcc cagcggattt aaagaaaagc ccatgcccgc
    10801 tctgatttcg tggcgcgtat tatcttccgt cggcagcgga ttgccgatgg ttgcatagta
    10861 tcgcgaatac ttgcgcagca gtaaattcgt actgaaattc cccagttgca aattgtgctg
    10921 cagagagacc gcatcgcctc ttttgtcgat ttcatccgaa gaacttccgg acaactgcac
    10981 ggtaaactgt ccggcaaggg gaacggcaaa ggttgtggag atgcctccgt tataaacgcc
    11041 atccgatcct tccgcactcg caccgatatt cactttattg gtcacgccat agcggtgaaa
    11101 agcggaaaag gccagatcgc cgtactcatt gctttctgat ccgtactgtt cccgaaggaa
    11161 acctgcatta tagctgtatt catgcaagcc cttgcgcagc atctgagcgc tgaaatacgc
    11221 catataggaa attcgttgaa catttccgaa cggatctttc agaacaactt caatgttgtg
    11281 agagcctgtc tgggaataaa tattttttaa atcgaatgat ccgggggcaa tgtcctgttt
    11341 cccgatcagg acgccgttca gatagatttc cgcctgtgtg ggataaataa ctgtgccctg
    11401 aagatccatg acgggttgcg tgataaaata cggatcgagt ctgtacacct tggaaaagcc
    11461 gatgcctccc atattcaccg tactgcccaa ttctccggaa ttggcgtact ggtcccccat
    11521 caccagccat tgcaaatctt tccggcgctc atacgtcgca ctgctttgaa gccgtacaag
    11581 atcataatcg gattccgttt ttgtaaaaac agaatccgat gtgaaaaaca tatctccggt
    11641 tctgaacccc agtttgttcg tgaccgacaa cgtctgaaat ccgccctgac cggtaaaagc
    11701 gtagttcacc ccgtaattga caaaggcgct cgtctctctc gggtaataga tatttttggt
    11761 ttcagcccgc agggaaaaaa gatcggcagc ggttctggtc gattccgtgg tcttgccgat
    11821 caccgttact gtgagtttct tttcgtcgaa tgtggacgtc acatccagaa gagcgttcaa
    11881 aggaacatgc ggctcatccc cgcgcagaat gacgatccgg tcttcggaat attgaagttt
    11941 cagagctttg gcatcgtccg gcctgatata cagattaccg cgatcatcga gctcagcaaa
    12001 gatatctccc ctggactgcg tattgataat aacactgacg acaatcgggg tggtttcttt
    12061 tggagtgaac gcagaggatt tcccggattc ggcaggaacc tgtcgcgcac tgcgagccgt
    12121 ttcttcggcc gtatatgccg gtgcggcaaa aaaggttatc agaaaaaaca cgaccgcaac
    12181 aagaaaaaat gtcttctcgc acgatccccg ccgcgataaa tatctggttg aaaaatccgt
    12241 aaagttattc gggacacatg ttcttttgaa cattcaatat tccgctgatg tctttgtctt
    12301 ccgttcggac attgatgtcg attttggcca gttgtccgca tacgtctttg ggaatttccg
    12361 cttcgtaaga ggatgacatc ccgtttaaga tataccagcc ggccacttcc ttggaataca
    12421 actctttccc atcggcggct ttcccgctga acgtgactga acgcagtttc acatgaacat
    12481 tgccggtgtt tttgacaacc gcccttgcct tcccggcggc catctcgact ttgtcgatcg
    12541 catatatttc ctgcggcttg agcggtttga cgaaaatcgg catggagaac cggaaggcga
    12601 tggtcacacc ggccttgacc ttttgattga cgtcaatatc ctgtgttttt ttctgggtgg
    12661 ggatttcctg aacaaaaagc ctgtatgttt tttctctggc gccgggcggt gttttgagtc
    12721 cgattctaac cgccctttga gaatttggat cgatactcat aaccttcggg aagaatacaa
    12781 tatcatttgt atcggtgtaa gcatctttac tgttctcgtc ctgattccat gctttaacgg
    12841 aaacctgaaa gtcgattttc tcgttactgt tattaatcac cgagaaaacc ccgcttttca
    12901 cttttccacc taaatccatt gtcgtcggtt gcacctgaaa gtcggcggcc aatgcatgcg
    12961 tagagtagat gattacgaac aatataacag ccaggaatcc gacaattttg ttcatgggtg
    13021 gatcaaaatt tttacaagtt tggagcgata tccttgtggc ccggtaatta tttttcatca
    13081 tataactccg attattttat ttttacaccc aaacgctgtt tctcaaataa cacagattga
    13141 cctgcactgc aagccgcaga ccttggccta atatgcaatc gtaactgtga cattgtccga
    13201 ataatccccg ggcggcgcac tttgatatgc tgacggcaca accgatatcc caacgggaat
    13261 ttccgatgcc ggcgccgcgc caaaaccatt tccggttatt ttccccgaaa ccccgcaggt
    13321 cgtgacattg taattaatgt tatccgttcc ttttttcaat tggcctgccg tggagccgcc
    13381 cgacgtttcg catgtcacat tgggttgtga cgcattggtg catttgacgg tcggcgacgt
    13441 gccgttggca gttgtacttg ccgtaatggt ggtattaaga gacggatcga tgtcgaaatt
    13501 catcgtgccg tttgtcggcg taccgcaggc gggaatcacc gtgacatccg aaacattgac
    13561 ataggccgaa agcgaaccgc cggcccagtt gatctcaacc gttatcggat tgctgaagga
    13621 agagacgcct tcaacaaaac cggcgtagct ggtaattgcc gaattcatgg tcagcaaagg
    13681 aatatccacg gcacccgcgc ctgtaccgtt agcatgcagg cccaacgtaa acggaacggt
    13741 atttgcgccg gtcaactgta attgacccac gggatttgta accgcaatag aatatgccaa
    13801 accggcgctg cattgaaaat taacctgctg ggtgattata ttattgacgg gagccggata
    13861 acctgaagga tcaagatttc cgaatgaaat gccccccgta tcggttccgt tcaaaacgca
    13921 gccggccgcg ttaatcacga ttgtggaaaa gcatattgga tccataccgg gctgtctgtg
    13981 gttaatcgca aaccagtaag tcccggcagt ggtcggcgtt cctgcaaggg tgacggaatt
    14041 cagggattga ttaattaaac tcaatccggg agggagcgac cccattacga tccgccacca
    14101 cggagacccg ccgtttccgt ttgttgctgt aaaggtatag gaataagcaa ccccgcgagt
    14161 cccttccgga agagttcccg gacagttaat tgctgcatgc ac
//
LOCUS       DAONQZ010000056        14193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4757_length_14193_cov_16.196775, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000056 DAONQZ010000000
VERSION     DAONQZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4757_length_14193_cov_16.196775"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLR13_08770"
     CDS             <1..342
                     /locus_tag="PLR13_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19471.1"
                     /translation="VVSRLAAELQRLKFKTGNDSESELFAVDTSGNRISHIFDVVTDA
                     KEKNVMAAAGRLMLQTSAGALNPLPVLVLPDDIMNHYKSDLRRIHICVIGFHWQDTQA
                     VFSGLEKISIR"
     gene            complement(427..2481)
                     /locus_tag="PLR13_08775"
     CDS             complement(427..2481)
                     /locus_tag="PLR13_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQC19472.1"
                     /translation="MKQASDKNQTDPAITTSKPERRRILIWGILCTLCFAVIFAFKPN
                     LMQRLDYLEYDFLLAKFPNNNASSKLVIVDLDEKSLSRYGQWPWPRYRVAGLLDKVIA
                     MQPDIISLDIVFAEPDRTSAGPLLKDLGDVYRIKIDLHGLPRSLKDNDQILAQTLAGG
                     PFVLGTIFHFDELKKGSDRCVLHPVKSSYINDDVNQNAHTEIPVSTGVLCNLPMLSDK
                     VSSSGFFNFSPDSDGVLRRLPMLIQYHGQLYPNLALATVLKLKNENHVILKKDRNHLQ
                     SIHYRGNAVPVDRHGQMLIKFRSSAQKYEYVSAADILDNNVSPERLRGRIVFVGTSAV
                     GLKELLNTPLGPVFPGVEVHATAVDNLLKGDFISLPGWSNGFILLLVMVSGLAMSLLI
                     SYQKAATGFVAMLLFAGGLWMTTHQVFFRMGFFVATAFPIASVICNYMFLTALKYRME
                     EKKNLAHMQELLLTQDITIESLANLTEHRHQETGGHIKRTRLYVRLLADHMKHRDKYR
                     TFLNDENIEMLDKSAPLHDIGKVGIPDKILLKPGKLTGEEFEIIKTHTTNGRNLIRSS
                     VRQFGKISFLNIAEEIAYTHHEKWDGSGYPQGLKGDAIPISGRLMALADVYDALISKR
                     VYKEAYPHAEAVEIIVNAKGKHFDPDMVDAFLEIHEQFRNIAREHADSQEAKESPKPD
                     AA"
     gene            complement(2504..3106)
                     /locus_tag="PLR13_08780"
     CDS             complement(2504..3106)
                     /locus_tag="PLR13_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC19473.1"
                     /translation="MKQLIGTVFCLGLVLVLAGCAVRSTVVLIPDPDGHVGQLVVANE
                     GGQQILNEKNQSVRVADLKTAPGKVKTMSAEEIQDTFADALAAEPLPPVKFILHFMPD
                     SDQLTDESRAVLPDIFQTIQSRQSTDIVISGHTDTVGEKNYNYTLSLERAQAIFKILE
                     AQGAVPAHITVTSHGEGNPLIKTADEVAEPKNRRVEVTIR"
     gene            complement(3186..3635)
                     /locus_tag="PLR13_08785"
     CDS             complement(3186..3635)
                     /locus_tag="PLR13_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQC19474.1"
                     /translation="MKRILILCLAMMFLLPCIAMPEESPVGKTKTSKGSVFVVRDGRQ
                     MPVAVGDRLFQNDTIRTGASSSVGIIFEDNTILSLGPDSEIVIDQYVFAPEKGVFSMI
                     ARMVRGTASYLSGIIGHQSPESVKFRTPEATIGIRGTHFLVKVDGCP"
     gene            4149..5732
                     /gene="cimA"
                     /locus_tag="PLR13_08790"
     CDS             4149..5732
                     /gene="cimA"
                     /locus_tag="PLR13_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQC19475.1"
                     /translation="MKNKILVYDTTLRDGTQGEQTNFSAEEKLRIAQRLDDVHFHYIE
                     GGWPGSNPKDARFFEMAKNAAFKNSRLTAFGSTRRAHTKTESCPNLKALIKAGTPACA
                     IVGKSWDLHVTDILKTDLNENLAMIADSIAYLKSMDKEVLFDAEHFFDGYKNNSRYAT
                     RVVKTALAAGADFIVLCDTNGGCLPHEICALVKRISAHVPFEKMAIHVHNDGGLAVAN
                     TLAAVQKGVAMVQGTVNGYGERCGNADLIAVIANLQLKMGKKCLPPESIKQLTNLSHY
                     ISDVANVPPLNSRPFVGRSAFAHKGGVHVSAVVKNSAAYEHIEPELVGNHRRVLMSDL
                     AGKSNVEYKAKELGIDLEKNDAVAKKVVHEVKLMEDRGFQFDAADGSLSLLIKKTIGE
                     FVAPFNLETFSVITSRKENNPCLSQATIKISVAGEEELTAAEGNGPVNALDHALRKAL
                     TKFYPQIKNMRLVDFKVRTLEGSEGTGAGVRVLVDSTDGEDVWSTIGVSENIIEASWQ
                     ALVDSIQYKLSKENNLNRR"
     gene            5898..6506
                     /locus_tag="PLR13_08795"
     CDS             5898..6506
                     /locus_tag="PLR13_08795"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQC19476.1"
                     /translation="MLFSINSQNPQMRLIKKAADILRDGGIVIYPTDTVYGLGCDLSN
                     KKGIEKIYELKRRNRKQPLSFVCSDLKHISEYAKVTDYAYKTMKRLLPGAYTFILEAS
                     RLVPKIILPKRSTTGIRVPDNQICLALVRELGQPIISTSVKTEKGETLGDPAVIKEYF
                     GKTVDLIIDGGIIVPEPSSVISLVDDNIDIIRKGKGDISAFL"
     gene            6614..8395
                     /locus_tag="PLR13_08800"
     CDS             6614..8395
                     /locus_tag="PLR13_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQC19477.1"
                     /translation="MLVNAVHKEQRRMATVNEKGILVDFNIEMPTKEPITGNIYKGKV
                     LKVERGLQAAFVDYGGSKDGFLPLKDVSHDYFTEPEKGQSNGKPALKVGQEIIVQAVR
                     EAIGNKGALLTSYISLPGRYLVLLPNKPGSGISRKVEDEEDRQKIKNMMKQIKIPETM
                     GFIVRTAGYNRTKQEISRDYQHLVRLWKEINKKAKSIGKTSMIYQETDFGVRSIRDYL
                     TSEIEEIWVDDLETFRKMRAYLRAISPRYIKLLKHYKEKTPLFDRHNLEDQIRVIYQE
                     RVELKSGGHIVINPTEAMITIDVNSGRGSNKKNIEETAFKTNIEACEEIARQLRLRDL
                     GGLIAIDFIDMMDKKHIAEVEKSFKKALSIDKARIQLSRISKFGILELSRQKKQSTIQ
                     EISYMKCPYCKGSGLRPSLEYAALGAFRKVESEAVKGLCCELKINLPHEIADYLLNRK
                     RSELLDLESSFDVTLTICGNSEIPWDRVDIQQIAKEHIDSADHPQEADEPKGQEAPAT
                     AEEEKAVPGGEKPARKRGRRRPRHKKRKPAESAPAGVLPDNVSDGAAEDGLSNLLIIQ
                     PAENQKDAASEKKASPDDPAAAPEDHK"
     gene            complement(8410..9228)
                     /gene="proC"
                     /locus_tag="PLR13_08805"
     CDS             complement(8410..9228)
                     /gene="proC"
                     /locus_tag="PLR13_08805"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQC19478.1"
                     /translation="MLKDKKAVIIGGGKMGGIIARSLVSRQIISAKNIHVTDIDASRL
                     KVLRSSLKIKTSSDNPRAAGESDIVILAVKPQNMAATLADIKSSMDKSKVVISIAAGI
                     TTKFIETHLGKGIRVVRVMPNTPALIGEGAAAVAAGRHAKAGDLRIARTIFNAVGISV
                     EVQEKLMDAVTGLSGSGPAYFFLIIEALIDAGVRNGLSRDLAKKLATRTMLGAARLCI
                     ESDKEPAELRAMVTSPNGTTFAGLNVMESKNIHAILAATVTAATRRSRELSKGT"
     gene            9599..10411
                     /locus_tag="PLR13_08810"
     CDS             9599..10411
                     /locus_tag="PLR13_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQC19479.1"
                     /translation="MLKYFYIESTKISCWTNTENVDAQKNNLVFIHGSGGNHSIWSHQ
                     YGKLHKRYNIAAVNLPGHGPSEGDGEQTIQNYCDWMRKILAALGLTKPVLVGHSLGAA
                     IALDFALRYPSDIAGIVCIGAGLKMPVNAFFLEYLQTHPPDIPAEIIDLICKFSLAKE
                     NRERLSGPLRKSLALSRVDVLYGDLLACNTLDLSHDAARISVPVFIVCGAEDKMTPPD
                     LSRALARQIGGAKLSMIEGAGHTVMIEKPVEFNNALEKFAESVFPASTPLKN"
     gene            10429..10725
                     /locus_tag="PLR13_08815"
     CDS             10429..10725
                     /locus_tag="PLR13_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQC19480.1"
                     /translation="MFVLSNFIIAVAKVADIVLTVYMWIIIFRALISWVNPDPYNQIV
                     IFLYRITEPVLAPIRRLIPMRNLGMDFSPVIVLLIIMFLKYFLVEVLIQAAQRM"
     gene            10756..11094
                     /locus_tag="PLR13_08820"
     CDS             10756..11094
                     /locus_tag="PLR13_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HQC19481.1"
                     /translation="MIHLTESKKGLTFDIHVTPHASRAEITSIQDGAFKVRVTAPPVE
                     GAANEACIKLLAQELGLKKSQMEIVAGAKSRKKTVTVKNISKTDLESKINGFLKANKI
                     CEQKSLLENL"
     gene            11120..12703
                     /gene="murJ"
                     /locus_tag="PLR13_08825"
     CDS             11120..12703
                     /gene="murJ"
                     /locus_tag="PLR13_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQC19482.1"
                     /translation="MTKTNRSENSQVARAAGIVGAATMVSRVFGLARDMVIAAFFGAS
                     WMTDAFWVAFRIPNMLRRLLGEGSLTVSFVPVFTEYLEKKTKEQALELAYNAITLLSI
                     ILAVISVLGIILSPLIVGLIAPGFISEPKQFALAVFLNRLMFPYIFFIALVALCMGIL
                     NSFRHFTAPALSPVLLNIAMIGAAFFLRPFFAEPITSLAIGVLIGGVLQLAMQWPFLR
                     KFGVKLKFRFNPKHPGIKQIGVLMIPAILGAGVSTINVFVGTILASLLPGGSVTYLFY
                     ADRIMELPLGIFAIAIGTATLPSFSKHVAAGNMDELKSGISFSLRLMLFLTIPAMAAL
                     MALNLPIISVLFQRGAFDVTSAVYTGQALFCYALGLWAFSVLRVFVSSFYSLQDSKWP
                     MKAAVIALIVNVVASLILMYPLKHNGIALASSIAATVNVLVLTIVLKRKIGKFLDRAF
                     YYSVFKIILSSVLMLFAIGVMEYVIPWNTHAGFKARLLYLSVAVTAGAATFFISAYLL
                     KSPEMQAFANIVKKRLSRP"
     gene            12805..14079
                     /gene="serS"
                     /locus_tag="PLR13_08830"
     CDS             12805..14079
                     /gene="serS"
                     /locus_tag="PLR13_08830"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQC19483.1"
                     /translation="MLDIKYLRQNIDFVRGKMDERGQKIDFDRFLNLDAKRRDILQAV
                     ETLRNERNSVSKQVGELKKKKEDASALIEKMSEVSARIKEYDESLRLTEEELNAFVMI
                     VPNVQHESVPQGQGSEDNTVVRTWGEKTAFNFEPKQHFDLGENLNILDFARGAKITGA
                     RFTLYRGAGAQLERAIFNFMLDLHTEQHGYTEVLTPFMVNAESMTGTGQLPKFKEDLF
                     KIEGMEYYLIPTAEVPVTNIHRDEILEEDMLPIYLVAYSPCFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFSKPETSYDELEKLTANAEEVLKRLGIPFRTVCLCTADLGFSSAKTY
                     DVEAWLPGQNTYREISSCSNFEDFQARRASIRFRRKESGKVEFVHTLNGSGLAVGRTV
                     VAVMENYQQADGSIVIPDTLRPYMRGLERIVP"
BASE COUNT         3524 a         3609 c         3681 g         3379 t
ORIGIN      
        1 gttgtcagcc gcctggccgc cgagctccag cggctgaaat tcaaaacggg caacgatagc
       61 gaaagcgagc tctttgccgt tgatacatcg ggaaacagaa tatcccatat ttttgacgtg
      121 gtgaccgatg ccaaagaaaa aaacgtgatg gccgccgccg gtcgattaat gttgcagaca
      181 tcggcaggcg cgttgaaccc tcttcccgtt ttggtgttgc cggacgatat catgaatcat
      241 tacaaatcgg atttgagacg aatccacatt tgcgttatcg gttttcactg gcaggacaca
      301 caggcggttt tttccggact ggagaaaatc agcatccgct aagatcgtcg tccggcatgt
      361 tgatttttta aaatgaattg gattgaggtc taacgcggaa aagataatgc ccctgcgtca
      421 gatgttttac gccgcatccg gcttggggga ctcctttgcc tcctgcgaat ccgcatgttc
      481 acgggcgatg tttctgaact gttcatgaat ttccagaaac gcgtccacca tgtcgggatc
      541 aaagtgcttc ccttttgcgt tgacgataat ctccacagcc tcggcatgcg gataggcttc
      601 tttatagacc cttttgctga tcagggcatc atagacatcc gccagcgcca tcagtcttcc
      661 gctgatggga atggcatccc ctttgaggcc ctgaggatag ccggagccgt cccatttttc
      721 atggtgcgta taggcgatct cttcggcaat gtttaagaat gaaattttac cgaattgccg
      781 gacggaggat cggatcaggt tgcggccatt ggtggtgtgt gtcttgataa tttcaaactc
      841 ttcgcccgtc aatttgcccg gcttgagcaa aattttatcg ggaatcccga ccttgccgat
      901 atcgtgcagg ggagctgatt tatcgagcat ctcaatgttc tcgtcattga gaaaagttct
      961 gtacttatcc cggtgcttca tgtgatcggc cagcagcctg acataaagtc gcgtgcgctt
     1021 gatatggccg cccgtttcct gatggcggtg ttccgtcaaa ttggccaggc tctcgatggt
     1081 gatatcctgt gtcagaagca gttcctgcat atgggccaga ttttttttct cctccatccg
     1141 gtattttaat gcggtcagaa acatgtagtt gcagataacc gacgcaatgg gaaacgcggt
     1201 ggccacaaaa aaacccatcc ggaagaaaac ctgatgggtc gtcatccata atccgccggc
     1261 aaaaagcagc atggcaacaa aaccggtggc ggctttctgg tagctgatca acaggctcat
     1321 ggccaacccc gacaccataa ccagcaggag gataaagcca ttggaccaac cgggcaggga
     1381 gatgaaatcc cctttcagaa gattgtcgac agccgtggca tgcacctcga cccccgggaa
     1441 tacgggaccc agcggggtgt tcaataattc tttcaggccg accgccgatg tgccgacaaa
     1501 cacgatccgg ccccgcaacc gttccggcga cacgttgtta tccagaatat ccgctgccga
     1561 tacgtactcg tatttttgcg cggaactccg gaacttgatc agcatctggc cgtgccggtc
     1621 aacgggcacg gcgttgcccc ggtaatggat ggattgcaga tgattgcggt cttttttcaa
     1681 aatgacatgg ttttcgtttt taagtttcag cacggtggcg agggccagat tgggataaag
     1741 ctgtccgtga tactgaatca gcatgggcag acgccgcagc acgccgtcac tatcaggact
     1801 gaagttgaaa aagcccgacg agctgacctt gtccgacagc atgggcaaat tgcacagcac
     1861 gcccgtactc accggaattt ccgtatgggc gttttgattc acgtcatcat ttatgtatga
     1921 cgatttgacg ggatgcagaa cacagcgatc cgaccctttc ttcagctcgt cgaagtgaaa
     1981 gatcgtgccg aggacaaagg gccctccggc cagagtctga gcaagaatct gatcgttgtc
     2041 cttcagactc cggggcagtc cgtgaagatc aattttaatc ctgtagacgt cgcccagatc
     2101 tttcagcagc ggtcccgctg acgttctgtc cggctcggca aaaacaatgt cgaggctgat
     2161 gatgtccggt tgcatggcga tcaccttgtc cagcagtccg gccacgcggt accggggcca
     2221 gggccattgc ccgtagcggc tgagactttt ttcatcgagg tccacgatga ccagtttgct
     2281 gctggcgtta ttgttcggaa attttgccag aagaaaatcg tactccagat aatcaagccg
     2341 ctgcatcaaa ttcggtttga aggcaaagat gaccgcaaag cacagggtgc agaggatccc
     2401 ccagatcaga atacgccgtc tttccggttt gctggttgtg atcgcggggt ccgtttgatt
     2461 tttgtccgat gcttgcttca tgaatgatca gtgcgaatgc tgtttatcgg atggtgacct
     2521 cgactctccg gtttttaggt tccgcaactt catcagcggt tttaatcagg ggatttcctt
     2581 ccccgtgcga ggtcacggtg atgtgggcag gcacggcgcc ctgggcctcg aggattttaa
     2641 aaatagcctg agccctttcc agcgacaagg tgtagttgta gtttttttcg ccgaccgtat
     2701 cggtatggcc tgaaatcaca atatctgtcg attgcctgct ttgtatggtc tgaaaaatgt
     2761 ccggcagaac cgcccgggac tcatcggtca gctggtccga atccggcata aaatgcagga
     2821 tgaatttcac cggcggcaga ggttcggcag ccagagcgtc ggcaaaagtg tcctggattt
     2881 cctcggcgct catggtcttt accttacccg gcgctgtttt caaatccgcg accctgaccg
     2941 attgattttt ttcgttcaaa atctgctgtc cgccctcgtt ggccaccacc aattgtccga
     3001 catggccgtc aggatccgga attaatacaa ccgtgctccg gaccgcgcag cccgccaaaa
     3061 caaggacaag ccccaaacag aatactgttc cgattaattg cttcatgtcc gcctccatca
     3121 tcagaaatgt ccggcaaatc gccgtccggc gaaaagcgtg tgattgaaaa tcgtatcacc
     3181 atgcgttacg ggcaaccatc caccttaacc aggaaatgtg tgccgcggat accgatggtc
     3241 gcttcaggcg tccggaactt gaccgattcc ggagactggt gaccgataat gcctgaaaga
     3301 taagacgcgg tccctctcac cattcgcgca atcatggaaa acaccccttt ttcgggggca
     3361 aacacatatt ggtcaatcac gatttcgctg tccggcccca aagacaagat agtattgtct
     3421 tcaaaaatga ttcccacaga gcttgacgcg cccgtgcgta tggtgtcatt ttggaaaagc
     3481 ctgtcgccga cggcaacggg catttgcctg ccgtcgcgga cgacaaaaac agagcctttg
     3541 cttgttttgg tttttcctac aggcgattcc tccggcatgg cgatgcatgg cagcagaaac
     3601 atcatggcaa gacaaaggat caagattctt ttcataggta gactccctta tggttgacgt
     3661 tgcggggcac ccgtatcaca ttcggaggat cgccctccgg atgtctcttt ttccttcgat
     3721 ggaaggtgtg tttttttaca aattatgtat atcataacag ccccccttaa aaaagaacaa
     3781 tctttaatcc ctatagaccg ataactgttt gcattaataa agttatcggt tttcatcgga
     3841 cgtaaggaga agctgttttc ccggtcccgt aaaaatcaaa tatgccggca atttttttgc
     3901 aaccattcat taaacacctg aaacaacatt tctattgaca aaatcatggg gtataattat
     3961 gatgcagtta aaattgatat caagagattt ttgatgaaga aagatattcg tgccatcatt
     4021 attatcaata ttataattag cttctacata ggaggccggg gcgcggtata gtatcagctc
     4081 gaaatcaaca aaattcaaat ccgctgccag gcaaacccgg cagcggattt tttttaggag
     4141 aggggacgat gaaaaataaa atactggttt acgatacaac cttgcgggac ggcacgcagg
     4201 gcgagcagac aaatttttcc gcggaggaaa aactgcgcat tgcccagcgc ctggatgatg
     4261 ttcatttcca ttatattgaa ggcggctggc cggggtccaa tcccaaagac gcgcgttttt
     4321 tcgaaatggc aaaaaacgcc gcatttaaga attcgcggct gaccgccttc ggcagcacgc
     4381 gccgcgcgca taccaaaacc gaatcgtgcc cgaatttaaa ggctttgatc aaggccggaa
     4441 cgcccgcatg cgccattgtc gggaaatcgt gggacttgca cgtcaccgat attctcaaga
     4501 cagacctcaa cgaaaatctg gcaatgattg ccgattccat cgcctatctg aaatccatgg
     4561 acaaagaagt tctctttgat gccgaacatt ttttcgatgg ctataagaac aattcccggt
     4621 atgccacccg ggtggtgaaa acggcgctgg ccgccggagc cgattttatc gtcctttgcg
     4681 ataccaacgg cggatgtctc cctcatgaaa tttgtgccct ggtgaagaga atttccgcgc
     4741 atgttccttt tgagaaaatg gccattcatg tacacaatga cggcggtctt gccgttgcga
     4801 atacccttgc cgccgttcag aaaggtgtcg cgatggttca gggaacggtc aacggttacg
     4861 gtgaacgatg cggtaacgct gatttgattg cggtgattgc caatctgcag ttaaagatgg
     4921 ggaaaaaatg tctgccgccg gaatccatca aacaactgac gaatctgtct cattatatca
     4981 gcgatgtggc caatgtgccg ccgctgaatt cccggccgtt tgtcgggcgc agcgcgttcg
     5041 cgcacaaagg cggcgttcat gtcagcgccg tggtgaaaaa ttctgccgcc tacgaacaca
     5101 ttgagccgga actcgtcggc aatcaccggc gcgtgctgat gtccgacctg gcgggaaaaa
     5161 gcaacgtgga atataaagcg aaagaactgg gcattgatct ggagaaaaac gacgccgttg
     5221 ccaaaaaggt cgttcatgaa gtcaagctca tggaagaccg cgggttccag ttcgacgccg
     5281 ccgacggttc gctttctctg ctgatcaaga aaaccatcgg cgaatttgtc gcgccgttca
     5341 atctggaaac cttcagcgtg attacgtcgc gtaaggaaaa caatccctgt ctgtctcagg
     5401 cgaccatcaa gatctcggtg gccggcgaag aggagttgac ggccgcggaa ggaaacggcc
     5461 cggtcaatgc gctggatcac gccctgcgca aagcgctgac gaaattttat ccgcaaatca
     5521 aaaatatgcg cctggtggat ttcaaggtgc gcacgctgga agggtcggaa ggcaccggcg
     5581 cgggtgtccg ggtgctcgtg gattcaaccg acggcgaaga cgtctggagc accatcggcg
     5641 tctccgaaaa tatcatcgag gccagctggc aggcgctggt ggacagcatt caatacaagc
     5701 tgagcaagga gaacaatctc aacagaagat gatgctcaac cttctttggg ctttcgtgtc
     5761 gggcgcggtg ccgcgcggaa cagacaaccg gggatgatca caaatcctgc atggcggacg
     5821 tcccgtgaag cttccgttgc gcaagaacct tttttggtat atgagtttct tcaaggttca
     5881 aaagggacgg acagagaatg cttttttcca tcaacagcca gaatccgcaa atgcgcctga
     5941 tcaagaaggc cgccgatatt ctgcgcgacg gcggcatcgt catttatcct accgacacgg
     6001 tttacggtct gggctgcgat ttgtccaaca aaaaagggat tgaaaaaatt tacgaactga
     6061 aacggagaaa ccgaaagcaa ccgttaagct ttgtctgttc cgatcttaaa catatcagcg
     6121 agtacgccaa agtaaccgat tacgcctata aaaccatgaa aaggcttctg ccgggagcgt
     6181 acacctttat tttggaggcc tccaggctgg tcccaaaaat tattttgcct aaacggtcaa
     6241 caacgggtat aagggtaccc gacaatcaga tttgtctggc gctggtccgg gaactcggac
     6301 agcccattat cagcaccagc gtgaaaacgg aaaagggaga aactctgggc gaccctgctg
     6361 tcattaagga atatttcggt aaaacagtcg atctgattat cgacggcggt atcattgtgc
     6421 cggaaccgtc aagcgtcatc agcctggtgg atgacaatat cgacatcata agaaaaggaa
     6481 aaggcgatat aagcgctttt ctctaaatat aaaacgcgga acggaatttt aacctgcaga
     6541 tgtttaaatg cgctgccgga gcgcgggtgc gcgcaaaaaa gccgccgcaa aaaggacatt
     6601 tatgaaacat attatgctcg ttaacgctgt tcataaagaa cagagacgga tggctacggt
     6661 taacgaaaag ggaattctgg tcgatttcaa tattgaaatg ccgaccaagg aaccgatcac
     6721 cggcaacatt tacaaaggca aggttctgaa agttgaacgc ggcctgcagg ccgcctttgt
     6781 ggattacggc ggcagcaaag acggttttct tcccctcaag gacgtcagtc acgactattt
     6841 caccgaaccg gaaaaaggac aatccaacgg caaaccggct ttaaaagtcg gacaggaaat
     6901 catcgtgcag gccgtccggg aagccatcgg caacaagggg gcgctgctca cgtcctacat
     6961 ttccctgccc ggacgatatc tggttttgct gcccaacaaa cccggcagcg gcatttcccg
     7021 caaagtggaa gatgaggaag accgccagaa aatcaaaaat atgatgaagc agataaaaat
     7081 ccccgaaacg atgggattta tcgtccggac ggcgggctac aaccggacca agcaggagat
     7141 ctcccgcgac tatcagcatc tggttcgtct ctggaaggaa atcaacaaaa aggcgaaaag
     7201 catcggcaag acctccatga tttatcagga aacggatttc ggcgtgcgca gtatccgcga
     7261 ttacctcaca tccgaaatcg aagaaatctg ggtggatgat ctggagacgt tccgtaaaat
     7321 gcgcgcctac ctgagggcca tctcgccgcg ttacatcaaa ctgctcaagc attacaagga
     7381 gaagacgccg cttttcgacc gccacaatct ggaagatcaa atccgcgtca tctatcagga
     7441 acgcgtggaa ctgaaatccg gcggacacat cgtcatcaac ccgacggagg ccatgatcac
     7501 gattgatgtc aattccggcc gcggctccaa caagaaaaat atcgaagaaa ccgcttttaa
     7561 aacgaatatt gaagcctgtg aagaaattgc ccggcagctc aggctacgcg acctcggcgg
     7621 gctgatcgcc atcgatttta tcgatatgat ggataaaaaa catatcgccg aggtggaaaa
     7681 atccttcaag aaggcgctga gcatcgataa ggcccgcatt cagctttccc ggatttccaa
     7741 gttcggaatc ctcgaattat cccggcagaa aaaacaatcg accattcagg aaatcagcta
     7801 catgaagtgc ccgtattgca aagggagcgg cttgcgtcct tctctggaat acgcggcgct
     7861 gggggccttt cggaaagtcg aatcggaagc tgtcaaaggg ctctgctgcg aattgaaaat
     7921 aaaccttccc catgagatcg ccgattacct gctcaatcgc aagagaagcg aactgcttga
     7981 tctggaatcg tcatttgatg tcacgctgac gatttgcggg aattctgaaa ttccctggga
     8041 tcgggtggac attcagcaga tcgcgaagga gcacattgac agcgccgacc atccgcaaga
     8101 ggcggatgag cccaagggac aggaggctcc ggcaaccgcc gaggaggaaa aggctgtgcc
     8161 cggcggggaa aaacccgcca gaaaaagagg acggcgcaga ccgcgtcata aaaaaaggaa
     8221 gcccgctgaa tcagcacctg ccggtgtttt gccggataac gtctccgatg gcgccgccga
     8281 ggacgggtta tcaaacctgc tgatcataca gccggcggaa aaccaaaaag acgccgcttc
     8341 tgaaaagaaa gcctctccgg acgaccctgc ggccgcgccg gaggaccata aataacaaac
     8401 ttgcgaagat tacgttcctt tagacagttc tctggagcgc cgggtggctg ccgtcacggt
     8461 cgcggcaaga atcgcgtgaa tatttttcga ttccatcacg ttcaatccgg cgaatgtcgt
     8521 gccgttgggc gatgtcacca tggccctgag ctccgccggt tccttatcgc tttcgatgca
     8581 cagacgcgca gctcccagca tggttcgcgt cgccagtttt ttcgccagat cccgggataa
     8641 tccgtttcgg accccggcgt caatcaacgc ctcgatgatc agaaaaaaat aggcggggcc
     8701 gctgccgctc aagccggtca ccgcgtccat gagtttttcc tgcacttcca cgctgatgcc
     8761 cacggcattg aagatcgtgc gcgcgatccg gaggtcaccc gctttggcat ggcggccggc
     8821 ggccactgcc gcggctcctt caccgataag cgccggcgta ttgggcatca ctctgaccac
     8881 acgaattcct ttgcccagat gcgtctcgat aaactttgtc gtgataccgg cggcaatcga
     8941 aatcaccacc ttcgacttat ccatagacga tttaatgtcc gccagcgtcg cggccatatt
     9001 ttgcggtttg acggcaagaa taacaatgtc ggattcgccc gccgcccgcg gattgtcgga
     9061 cgaggtcttg atcttcaggg acgaccgcag aaccttgagt ctcgaggcat cgatatcggt
     9121 aacgtgaatg tttttggcgg aaatgatttg acggctcacc aggctccggg cgatgatacc
     9181 gcccatttta ccgccgccga tgatcaccgc ttttttatcc ttcaacatac gctcaatgcc
     9241 tccatgcctt ggtatgccgt tcattatcct gcttacgccg gtcaggtcaa gcggtttatc
     9301 atgatagcca aacttcagaa atggagtgtg ccgaagtttg tccctaccgc ctgaaggtgg
     9361 cgggatagtg aacctgtgga tcccgaatgg gatcccaaag agaactatcc ccgaagctga
     9421 gctgagcggg atagttcgcg ctaagatttt ccgggcactt cgtttcggcg cagtgccctt
     9481 cagggtaaaa atcaaggctc attaaattat cccgcgcagc ggagggcggc aaatccatga
     9541 ttcttccgtt gcattcgatt ttgtttttgt gatagttaac gggtgaaagg gtgacggcat
     9601 gctcaaatat ttttatatcg aatcaacgaa gatttcctgc tggaccaata cggagaatgt
     9661 tgacgcgcaa aaaaataatc tggtttttat tcatggctcc ggcggcaatc acagcatctg
     9721 gtctcatcag tacggcaaac tgcacaaacg atataacatc gccgctgtca atcttccggg
     9781 gcatggtcct tccgaaggtg acggcgagca gacgattcaa aactactgcg actggatgag
     9841 aaaaatcctt gcggcgctcg gtctgacaaa accggtgctg gtgggacatt cgctgggcgc
     9901 ggccatcgcg ctggattttg ccctgcgcta tccttcggat attgccggca ttgtctgtat
     9961 cggcgcggga ttgaagatgc cggtgaatgc tttcttcctg gagtacctgc agacccatcc
    10021 gccggatata ccggctgaaa tcatcgacct gatctgcaaa ttttcactgg ccaaagaaaa
    10081 tcgcgaaagg ctttccgggc ctctgcgcaa aagccttgcg ctttcccggg tggatgtttt
    10141 atacggcgac ctgctggcct gcaacacgct ggacttatcg cacgacgcag ccaggatcag
    10201 cgttcccgta ttcatcgttt gcggtgcgga agacaaaatg acgccgccgg atttatcgcg
    10261 ggcgcttgcc cgacagatcg gcggggcgaa actgtccatg attgaaggcg ccggtcatac
    10321 ggtgatgatt gaaaaacctg tggaattcaa caatgctctg gaaaaatttg ccgaatccgt
    10381 tttcccggct tcgacgccgt taaaaaatta aatgacagga gacgcgttat gtttgttttg
    10441 agcaatttta tcatcgctgt ggccaaagtc gccgatattg ttttgacggt ttacatgtgg
    10501 atcattatct tccgtgccct gatctcctgg gtcaatcccg atccctacaa ccagatcgtg
    10561 atcttcctgt atcgcatcac ggaacccgtt ctggcgccga tacgacgact gattcccatg
    10621 cgcaatctcg ggatggattt ttcacccgtt attgttttac tgatcatcat gtttctgaaa
    10681 tattttctgg tcgaagtact gattcaagcg gcacagcgaa tgtaattttt ttgaaacact
    10741 atttatgaag gcaccatgat ccatctgacg gaatcaaaaa aagggctgac attcgatatt
    10801 catgtgacgc cgcacgcctc ccgcgcagaa atcacaagca ttcaggacgg tgcttttaaa
    10861 gtccgggtga cggcgcctcc cgtggagggc gcggccaatg aggcctgtat caagctgctg
    10921 gcccaggaac tggggctgaa aaaaagtcag atggaaattg tggccggcgc taaatcaaga
    10981 aaaaaaaccg tgacggttaa aaacatcagc aagaccgatc tggagtcaaa gatcaacggt
    11041 tttctgaaag caaacaaaat ctgcgagcag aaaagtcttt tggaaaacct gtaaaggatc
    11101 aaacaacaaa caaatctcta tgacaaaaac aaatcgttcg gaaaattccc aggtcgccag
    11161 ggctgccggc attgtcggcg ccgccaccat ggtcagccgc gttttcggac tggcacgcga
    11221 tatggtcatt gccgcatttt tcggcgccag ctggatgacg gacgccttct gggtcgcctt
    11281 ccgcatcccc aacatgctca ggcgccttct gggtgaagga tcgctgacgg tttcgtttgt
    11341 tcctgtcttt accgaatatc tggagaagaa aacaaaagaa caagccctcg aactggctta
    11401 taacgctatt acccttcttt ccatcattct cgcggtcatt tcggtgttgg gaattattct
    11461 gtcgccgctg attgtcgggc tcatcgcgcc cggtttcatt tccgaaccga aacagttcgc
    11521 gctcgcggtt ttcttaaacc gtctgatgtt tccctatata ttttttatcg ccctggtggc
    11581 cttatgcatg ggaattctga attccttccg gcattttacg gctcccgcgc tgtctccggt
    11641 attgcttaac atcgccatga tcggggcggc cttttttttg cggccttttt ttgccgaacc
    11701 gatcacatcg ctggcgatcg gtgttttgat cggcggcgtg ttgcagttgg ccatgcaatg
    11761 gcctttttta aggaaattcg gcgtcaaatt aaagttccgt ttcaatccga aacaccccgg
    11821 cattaaacag ataggcgtgc tgatgatacc ggccattctc ggcgccggag tatccaccat
    11881 taatgttttc gtgggaacga ttctggcttc tctgctgccc ggcggctccg taacgtacct
    11941 attttacgcc gaccggatta tggaacttcc gctgggaatt ttcgccattg ccatcggaac
    12001 ggcgacgctg cccagttttt ccaaacatgt cgccgcggga aacatggacg aattaaaatc
    12061 cggtatttcc ttttccctgc ggctgatgct ttttctgacc attccggcaa tggccgccct
    12121 gatggcgctg aatctgccga tcatttctgt tttgtttcag cgcggcgctt ttgatgtgac
    12181 ctccgctgtt tatacgggac aggcactgtt ttgttacgct ctggggctgt gggctttttc
    12241 cgttctgcgt gtttttgttt cctctttcta ttccctgcag gattccaagt ggccgatgaa
    12301 agcggccgtg atcgcgctga ttgtcaatgt cgtggccagt ctgatcttga tgtatccttt
    12361 gaaacacaat ggcatcgcgc tggccagttc catcgctgca accgtcaacg ttctggttct
    12421 gacgattgtt ctgaaaagaa aaatcgggaa atttctggac cgcgcttttt attattctgt
    12481 tttcaaaatc atcctgtcct ccgtgttgat gcttttcgcg atcggggtga tggagtatgt
    12541 catcccctgg aacacccatg ccggcttcaa ggcaagactg ctttatctct cggttgcggt
    12601 cacggccgga gcggcaacat ttttcatcag cgcctacctg cttaaaagtc cggaaatgca
    12661 ggccttcgcc aatatcgtca aaaagaggtt gtcccgtccc tgatcttgaa tcccctgaca
    12721 aaattattga cttcaacatc ccaaagtgat aggaaattca gtcttttaaa agacatggca
    12781 agtaaaaacg tgcaggttta atttatgtta gatatcaagt atttaagaca aaacatcgat
    12841 tttgtccgcg ggaagatgga tgagcggggc caaaaaatcg atttcgaccg ttttttgaat
    12901 ctggacgcca agaggcgcga tattctgcag gcggtagaaa cgctccgcaa cgaacgcaac
    12961 agtgtttcca agcaggtcgg tgagctcaag aagaagaaag aagatgcttc cgcgctgatc
    13021 gaaaagatga gcgaggtgtc ggcccgaata aaagaatatg acgagagcct gcgcctgacg
    13081 gaggaggaac tcaacgcctt cgtcatgatt gtgcccaatg ttcagcacga atcggttccg
    13141 cagggtcagg gctccgaaga caacaccgtc gtgaggacct ggggtgaaaa aacagcattc
    13201 aacttcgagc cgaagcagca tttcgatctc ggggaaaatt taaatattct cgatttcgcg
    13261 cgcggcgcaa aaatcaccgg agcgcgtttt acgctttacc gcggggcggg tgcccagctg
    13321 gaacgggcga tttttaattt catgctcgat ctgcacaccg agcagcacgg ctacacggaa
    13381 gtactgacac cattcatggt caacgccgaa agcatgaccg gaaccggcca gctgccgaag
    13441 ttcaaagaag atctttttaa aattgaaggc atggaatatt acctgattcc gaccgccgag
    13501 gttccggtca ccaatattca ccgggatgag attctggaag aagacatgct gccgatttat
    13561 ctggtagctt attcgccctg cttccgggcg gaagccgggt catacggcaa ggacacgcgc
    13621 ggactgatcc gccagcatca gttcaataaa gtggagatgg tcaagttttc caaaccggaa
    13681 acatcctatg acgagctgga gaaactgacg gccaacgcgg aagaagtgct caagaggctg
    13741 ggcattccct tccggaccgt ttgtctgtgc acggcggatc tgggtttttc atcggcgaaa
    13801 acgtacgatg tggaagcatg gctgcccgga caaaatacgt atcgggaaat ttcatcgtgc
    13861 agcaattttg aagactttca ggcgcgccgc gcatcaatcc gctttcgccg gaaggaaagc
    13921 ggcaaggtgg aatttgtcca cacattgaat ggttcgggac tggcggtggg cagaacggtc
    13981 gtagcagtca tggaaaatta tcagcaggca gacggcagca ttgtcatacc ggacaccctg
    14041 cgcccctata tgagaggtct tgaacgcatt gttccttaag atggtctgaa ggataatcga
    14101 aggaattttt taaaatccgg agggatggcc gagtggtcga tggcggcggt cttgaaaacc
    14161 gttgaccgaa aggttcgcgg gttcgaatcc tgc
//
LOCUS       DAONQZ010000057        14166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4777_length_14166_cov_14.760541, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000057 DAONQZ010000000
VERSION     DAONQZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_14166_cov_14.760541"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..2147
                     /gene="fusA"
                     /locus_tag="PLR13_08835"
     CDS             63..2147
                     /gene="fusA"
                     /locus_tag="PLR13_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQC19484.1"
                     /translation="MAKYETKSIRNLAVVGHGGTGKTSLCESILYVSGKSERLGRVDD
                     ATSCMDYEPEEQKRRGSISSAANFAEWQKHKINFIDTPGDSNFAYDIKCSMSVVENAV
                     VVVDAVGGVEFQTQKVWELADEFKLPRIIFINRLDRERANFNAALDSIKNKLKRKATP
                     VCLPIGSEDQFNGIVDLIGMKAYLFSDNKGIGKAVDVPADMKDDVKLLHDSMVEDIAE
                     SDDELMNKYLESGELSDEELKAGLKKGVASGSFIPVICGSAVKGIGISMLLDLCVDAF
                     ASPVDRGAIKGTKPGTDNVEERQPNEDTPFSAIVFKTIADPYAGRLTLFRVYSGKLHS
                     DSPVYNSTRKMTEKFGHIFFLEGKNQKQTEELIPGDIAGVAKLKETTTGDTLCNEKSP
                     IIFEKVPVPPPIMSFAIEPKSRGDEDKIASSIHRLTEEDPTIQFTRNVETKEMILSGM
                     GQVHIEVTIEKMKRKFGVEVNLNTPKIPYKETIKGKTNVQGKYKKQSGGRGQFGDCWI
                     DIEPLPRGGGFEFHDKIVGGVIPGQYRPAVEKGIVEAAAKGVIAGYPVVDFKINLTFG
                     SYHTVDSSEMAFKIAGSMAFQKGVMDCQPILLEPIVNIEIEVPDEYMGDVIGDLNSRR
                     GRVQGMDAKGSNQIIKGQVPLAEILKYAPDLRSMTSGRGNFTYTHSHYEEVPAFIAEK
                     IIAAYKKDKEEE"
     gene            2164..2655
                     /gene="mog"
                     /locus_tag="PLR13_08840"
     CDS             2164..2655
                     /gene="mog"
                     /locus_tag="PLR13_08840"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HQC19485.1"
                     /translation="MISAGIITVSDKGSQGQRDDLSGPAIAEMLAGAAIEIRHTIIIP
                     DEKDQIRKTIIDCSDVKQLDLILTTGGTGVSPRDVTPDATLDVIDKQIPGMAEAMRHE
                     SMRVTPHAMISRAVAGIRGKSLILNLPGSPKSVRENLAVVLPALKHAIEKIKGDDSEC
                     AVP"
     gene            complement(2621..3136)
                     /locus_tag="PLR13_08845"
     CDS             complement(2621..3136)
                     /locus_tag="PLR13_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HQC19486.1"
                     /translation="MMSKHPSHNKGPAETEVRFITDASLAGLAKWLRVLGYDTIVFHQ
                     TAGRSMMRCADKENRILLTRRADMVERQFSGSLFLIKDLEVGKQLKAVIERFALKIDR
                     DKMFGICLICNEKLLPVSREKVRDLVPPYVFENCSRYNECPRCKKIYWMGTHSRNAFR
                     FMEQHIPNHLP"
     gene            complement(3111..3419)
                     /locus_tag="PLR13_08850"
     CDS             complement(3111..3419)
                     /locus_tag="PLR13_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQC19487.1"
                     /translation="MRVVYLILVILFSFLIVTFSLQNSARVQLQYYHYLNIDVPTYMI
                     IFASLLIGVIITAFWGIVERFRLNRTINRLNKLVRDLRREIQATEKPLMINDVKTPQP
                     "
     gene            complement(3421..3906)
                     /locus_tag="PLR13_08855"
     CDS             complement(3421..3906)
                     /locus_tag="PLR13_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQC19488.1"
                     /translation="METIFAPWRMEYIRGEKVNGCVFCKCSVRCDEYILFEGKTAYVM
                     MNKYPYVNGHLMIIPQRHVGQIEDLTQEEKKEIFDLLDASVKVLKEAMNPNGFNIGIN
                     IGKAAGAGVEEHFHVHVIPRWEGDTNFMSVVTNVRVIPEALDETVASLTPLFKKYCRE
                     A"
     gene            complement(3933..4424)
                     /locus_tag="PLR13_08860"
     CDS             complement(3933..4424)
                     /locus_tag="PLR13_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQC19489.1"
                     /translation="MEGKKIKIGVISDTHLDDYDPKMQKRIAGYFDDADMILHAGDMV
                     DLNVLKMFGDKDVKAVCGNMDSSSVRKTYPDHLLFEIKGFKILLIHGWGSPWGLEEKI
                     AARFKNVHCIVYGHTHRPSNHLENNVLFFNPGSAADSSFNSSKTIGILEIDKQITGRI
                     IKI"
     gene            4638..4919
                     /locus_tag="PLR13_08865"
     CDS             4638..4919
                     /locus_tag="PLR13_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19490.1"
                     /translation="MNNLEKTLNKIAENILHFDEASLTSLWAQYKNKVENFSPSAEWE
                     KAVIIFSIINAVRAKNAIFNDNLLHKKSSAKAEKPQKKIKTKSYLTLVK"
     gene            4919..6928
                     /gene="ligA"
                     /locus_tag="PLR13_08870"
     CDS             4919..6928
                     /gene="ligA"
                     /locus_tag="PLR13_08870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQC19491.1"
                     /translation="MDKSSDLKRIIDLRKTIEYHNRRYYQQDSPEISDAEYDRLMREL
                     QDLENRYPEAFSPSSPSQRVGAAPLSKFAPFHHPSPMFSLANAFSEGEIIEFDQRLKR
                     LAGVNDIGYIAEPKLDGLAVNLIYENGVFKQGATRGDGTTGEDVSQNLKTISSLPLFM
                     KKTSEYQLPSFIEIRGEVYIEKDPFEKLNQRRTQDGEEPFANPRNAAAGSLRQLDSRI
                     TARRPLNIFLYGIGEAKGIDFSTHWEALQILDGWGFPVNRVIEKASNIEACIHYFERI
                     AAQREKLPYEIDGVVLKVDDLSLQKILGNVSRNPRWALACKFPAAQAMTVVKDIIVGV
                     GRMGTLTPVAIMEPVNVGGVMVSRATMHNEDEVLKKDVRVGDTVVIQRAGDVIPEVVR
                     IVPEKRSSDTKQFKMPAHCPECGSEVVRYEGEVAHRCVNISCPAQLKEHIRHFASRGA
                     MDIEGLGEKVSAQLFDAKLIADPADLYFLTKEQLVDLDRQAEKSAQNLIEAIAGSKNP
                     SLDKFIYALGIRHVGERTAKLLANHYGSIKNLISTTQDELTAIHEIGPEIAESIVDFF
                     HEQKNINVLNKFFKAGINPQKKEVQTSAPLQGKSFVFTGTMENMGRNEAQTIVENLGG
                     TIHSSVTKKTTYVVAGSDPGSKLDKARSSGIQIISENEFLKLIGR"
     gene            6931..7203
                     /locus_tag="PLR13_08875"
     CDS             6931..7203
                     /locus_tag="PLR13_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQC19492.1"
                     /translation="MKQIHVHVRGRVQGVFFRAATQETARGLNLTGWVRNRSDGSVEA
                     VFEGEDDHVDIMLAWCRQGPPNARVEEVLIQEEPCSGLFLDFDIKY"
     gene            complement(7307..>10908)
                     /locus_tag="PLR13_08880"
     CDS             complement(7307..>10908)
                     /locus_tag="PLR13_08880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQC19493.1"
                     /translation="FLIGAYRDNETNDAHPLTLALDHIRKKGKKINSIVLGPLNLTGI
                     NMIIMNVLLCDAEKSMPLAELINRKTAGNPFFVIQFLKNIYDNRLLQLNPSTGWEWDI
                     DKIRQMQVTENVIEFMAEKISHFPKNTREILKICACIGNRFDLELLSVVSGKSIDETL
                     FDLTSAIQEDMISLHGNMYKFMHDRIQEAAYSMISEEEKSAMHYRIGGNVLKKTDEKN
                     LHEKIFYIVDQLNRGIRCVSGTDEEIMLARLNLQAAQKSKKSTAYGSASFYLKTAMGL
                     LPEYAWKSHYELTYAIYKEAIECEYLNLNFSEAEKIFHAVVNNARSNIDKANVYTLMI
                     ILYTTQGNYDAALHVGMEGMKMLGIHLPDNVSDVRVGWELLKLRLKFGRRKIEDLIDL
                     QYIPMPNTLDEAKQMAAEYLRVHPGKSLASPEINLWEMLSYAYLAIHTGTVAFYANPN
                     LFAFIAINGVYRLLDYEINFEYSPFAYIALGSIVGSSLGFYQHGYRFGVAALKTNEKI
                     ADTKNRCRIEFAFPMFIQHWKKHARKDLDYFRNAYKNGIENGDLIFSGHNVNLIGMTR
                     IMLGDNLDDILEEYGKYRDFQLGGKDPFIARNYMENTRMCLCLKGRTESRGSLNGDGF
                     DAKEQAGYYKSENNLLGTFYFSLVRLRINYLFGNYSACRDLLPEMRRIVQKKTALGNL
                     HIPEFYLYSSLTMTASYPEADPMTKARYKMSLRANQMKMLIWAKACPENFQHKYDLVA
                     AEMMKNKGRFREAQKLYHKAIEGARENGYRQEEAIACERLALLYLDHRCRDEAGFFMQ
                     KAHKGYLGWGASEKARDLEEKYPSLVPLEQKQWGTGTVTVSGPSGSMTISGATETTGS
                     TKMLDLSTAMRVSQIIASEIMLDRLLQKIMHESITNAGAQRGYLLLESDGDLTIEASQ
                     DVDKGESQVMQSLPLKDCPDICHSIVNYVYHSGEDIVLGNAVKEGLFTNDPYVIRVRL
                     KSILCTPIMSKGKISGILYMENNISENAFTPERLEVLRSFSVQAAISIENARLFELAT
                     TDGMTKLYVHRYFQLLLDQEIKRSRRQDRKFSLVMMDIDNFKSFNDTYGHQLGDRVLK
                     DVAAAARKASRSEDVAARYGGEEFVMILPDTDSKQAVILAERIRAGVAAIEIPHDHQK
                     LHVTISLGVSTYPDHSEIKEELIRAADAALYESKHRGKNCVSVFEKKNEINLQ"
     assembly_gap    10909..11008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11009..12172)
                     /locus_tag="PLR13_08885"
     CDS             complement(<11009..12172)
                     /locus_tag="PLR13_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQC19494.1"
                     /translation="MEKFANYILSEKIHETRNSVIFRGYKETEGLPLIIKLLKTINPT
                     SSEIARFKQEYNLVKSLDVDNIVKIFDLVDYDNQYAIIEEDFGGISLKDSLKDKELDL
                     QSLLRISSKVSGTLGLIHKNNIIHLDIKPDNILINPERDEVKVADFGISAVLTHANDE
                     LYNPDVIEGTLSYMSPEQTGRMNRGVDYRTDIYSLGVTFYEMLTGEVPFKSKDPMELI
                     HSHIARQPLPPDHLNPSIPPVLSSIILRMLSKNPEERYQNCLGIMADLDECLRQLNQK
                     GQVDDFPIATKDISIRFNIPRTLVDRDNERIELMRCFERTCKGLSEFMLVTGQPGIGK
                     SALVNEIYKPIIARKGYFIFGKYDQFRKDVPYSAIIQSFQGLIRQIISESEEKI"
     gene            complement(12285..13382)
                     /locus_tag="PLR13_08890"
     CDS             complement(12285..13382)
                     /locus_tag="PLR13_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQC19495.1"
                     /translation="MKKCSACGEVQEANAIFCTNCGQRLAEDSNVKKCPSCGTSNKPE
                     DAFCSDCGSRLEGPKAQASESSPVATQLLALNNEFLSVRESSAGQFEFSSETGARSAV
                     QHVKIKYTALAILDPLTKQITFWEKMVESSAGMTAGVFAEKTVQKGIDVGKKIHGQLL
                     FGGKYGFEYGKLKDVVKDIAAKHGWNFKLNIFKPKSKTETTGLINKIPFIKVLGPIFA
                     ILLIMAIGLIAYFTLSGDSSKTTSQAGLESHDSDEVGKAGSSIFGKSKTEADSSKQII
                     QTDRDTYRSGEKIQVHYYNAPGHSRDWICIVPAGSRNTDAGDYQYIPRRGQGVLTFRS
                     PGPGRYEARAFYRYSPGRYRITARYTFTVEG"
     gene            complement(13478..>14166)
                     /locus_tag="PLR13_08895"
     CDS             complement(13478..>14166)
                     /locus_tag="PLR13_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQC19496.1"
                     /translation="IGGYFIGKGGTRFKSDTRTVTVKGLVEKVVKADQSIWVLRFRRA
                     SEDIKDAQTKISADRDAGIAFLKKQGFKDEEISRQPTRTIDKLAREYDQSQATERYRY
                     VVTSSLVVTTSNIDLVIKALGETEELLKSGVVLDGQDEGTANPRYVITKFNELRPPLL
                     AEATKNARMTAQQFASDSGARVGKIRSANQGNIQIFGSDGNDESAPYSPTSTPVKKIR
                     VVSTFEFELI"
BASE COUNT         3724 a         3389 c         3184 g         3769 t
ORIGIN      
        1 tatacgttaa acagcccaaa ttcaatttga attgtctttt taaagcattg aaaggagttt
       61 tcatggcaaa atacgaaaca aaatcaatca gaaatttagc tgtggtcggt cacggcggaa
      121 ccggaaagac atctctttgt gaatccatat tgtatgtttc agggaaaagt gaacgattag
      181 ggcgcgttga tgatgcaacc tcctgcatgg actatgagcc tgaagaacaa aaacggcggg
      241 gctccatcag ttccgcggca aattttgccg aatggcaaaa gcacaaaatc aattttattg
      301 atacgccggg tgactccaat ttcgcctatg atataaaatg ctccatgagc gttgtggaaa
      361 atgctgtcgt cgtggttgac gctgtcggcg gtgttgagtt ccagacacaa aaagtttggg
      421 aacttgccga tgagttcaaa ctaccccgta ttattttcat taacagactg gatcgggaac
      481 gcgctaattt caacgccgcg ctcgacagca tcaagaataa attaaagaga aaagcaaccc
      541 ccgtctgtct tcccatcggt tccgaagatc aatttaacgg cattgttgat ttaatcggca
      601 tgaaagctta cctgttcagc gacaataagg gcatcggcaa agccgtcgat gttcccgcgg
      661 acatgaaaga cgacgtaaag ctcctgcacg attccatggt ggaagacatt gccgaatccg
      721 atgatgaact gatgaataaa tatctggaaa gcggtgaact ttccgatgaa gagttaaaag
      781 ccggattgaa aaaaggcgtt gcgtcgggaa gtttcattcc ggtcatttgc ggctccgcgg
      841 ttaaaggcat cggcatctcc atgcttctgg acctgtgcgt ggatgctttc gcatcgcctg
      901 tcgatcgcgg tgcaatcaag ggaacgaaac cgggaacgga caacgttgaa gaacgccagc
      961 ccaatgaaga tacgcccttc tcggctatcg ttttcaagac gattgccgat ccttacgccg
     1021 ggaggctgac tctcttccgt gtttattccg gaaagcttca ttccgattcg ccggtttata
     1081 attcaacccg gaaaatgacg gaaaagttcg ggcatatctt tttcctcgaa gggaaaaacc
     1141 agaaacaaac ggaagaattg attcccggcg atatcgccgg cgtggccaaa ttaaaggaaa
     1201 caacaacagg cgataccctc tgcaatgaaa aatctcccat cattttcgag aaagtccctg
     1261 ttcctccgcc gatcatgtcg tttgcgatcg aaccgaaatc gcgcggcgat gaagacaaga
     1321 ttgcttcctc cattcaccgg ctgacggaag aagacccgac cattcagttc acccggaatg
     1381 ttgaaaccaa ggaaatgatt ctctccggca tgggacaggt gcatattgaa gtcaccatcg
     1441 aaaaaatgaa aagaaaattc ggcgttgaag tcaatctgaa cacgcctaag atcccctata
     1501 aagaaacgat taagggaaaa accaacgtac agggaaaata caagaagcaa tccggcggac
     1561 gcggacagtt cggcgattgc tggatcgata tcgaacccct gccccgcgga ggcggttttg
     1621 agtttcatga caaaatagtc ggtggcgtta ttccgggaca atatcgtccg gccgttgaaa
     1681 aaggcattgt ggaagcggcg gccaaaggcg tgatcgccgg ttatccggta gtggatttta
     1741 aaataaatct tacattcggt tcttatcaca cagtagactc gtcggaaatg gcctttaaaa
     1801 tcgccggttc catggctttt caaaaggggg tcatggattg tcaaccgatt cttctggaac
     1861 ccatcgtcaa tatcgaaatc gaagttcccg acgaatacat gggggatgtg atcggagacc
     1921 tcaacagccg acgcggccgc gttcagggca tggatgccaa aggttccaac cagattatca
     1981 aaggccaggt tccgctcgct gaaattttaa agtacgcacc ggatctcaga tccatgacca
     2041 gcggcagagg caatttcacg tacacgcatt ctcattacga ggaagttccc gctttcatcg
     2101 ctgaaaaaat cattgccgcg tataagaaag ataaggaaga agaataatca caggaatcag
     2161 ctcatgatca gtgccggaat tataacagtc agtgacaaag gctctcaagg ccaacgtgac
     2221 gatttaagcg gccctgccat agcagaaatg ctggcaggcg ccgccattga aatcaggcac
     2281 accatcatca ttccggatga aaaagaccag atcagaaaaa caatcatcga ttgttcagat
     2341 gtcaaacagt tggatttgat tctcaccacc ggaggcaccg gtgtcagccc ccgcgatgtg
     2401 acgcccgatg cgacactgga cgtcattgac aaacaaatcc ccggcatggc ggaagccatg
     2461 cgccatgaga gcatgcgcgt tacgccccat gccatgattt cccgcgccgt ggcgggaatt
     2521 cgcggaaaaa gcctgatatt aaatcttccc ggaagtccca aaagcgtccg tgagaatctt
     2581 gcggttgtcc tgccggcgct gaaacacgcc atcgaaaaaa tcaagggaga tgattcggaa
     2641 tgtgctgttc catgaagcgg aaggcgtttc gggaatgcgt tcccatccag tagatttttt
     2701 tgcaacgggg acattcattg taccgggaac agttttcaaa gacataaggc ggaaccagat
     2761 cccgtacctt ttcccgcgaa accggaagca gtttttcatt gcagatcaga caaatcccga
     2821 acattttatc cctgtcgatt ttcaaggcga atcgctcgat gaccgccttg agttgcttgc
     2881 cgacctcaag atctttaatt aaaaaaagac tgccggagaa ctgccgctca accatgtccg
     2941 ctcttctggt caacagaatt ctgttttcct tgtccgcaca gcgcatcatg gaacgtccgg
     3001 cggtctggtg aaagacaatc gtgtcatacc ccagaaccct tagccatttg gccagaccag
     3061 ccagactggc atccgtgata aaccggacct ccgtttcagc tggacctttg ttatggctgg
     3121 ggtgttttga catcattgat catcagcggt ttttcggtgg cctgaatctc cctgcgcaaa
     3181 tcgcggacca gtttatttag gcggttgatt gtgcggttga ggcgaaaacg ttccacgatg
     3241 ccccaaaacg ccgtgataat aacgccgatc agaagagatg cgaagatgat catataggta
     3301 ggtacgtcga tgttgagata gtgatagtac tgcaattgaa ctctggccga attctgcaga
     3361 gaaaacgtaa caattagaaa agaaaaaaga atgactagga tcagataaac gactctcatt
     3421 ctatgcctcc ctgcaatatt ttttaaaaag aggcgtaagg ctggcaacgg tttcatcgag
     3481 tgcttccgga atgacccgga cattggtaac gacgctcatg aaatttgtat cgccctccca
     3541 tcgcggaatg acatgcacgt gaaaatgttc ttcgacgcct gccccggcgg ctttgccgat
     3601 attgatgccg atattgaatc cgttggggtt cattgcctct ttgagcactt taacggaagc
     3661 gtccagaaga tcaaagatct cttttttctc ctcctgcgtc aaatcttcaa tttgcccgac
     3721 atgccgctgg gggatgatca tcagatgtcc gttcacataa gggtatttgt tcatcatcac
     3781 gtaagcggtt ttgccttcaa acaaaatata ctcgtcacat cggacggaac acttgcagaa
     3841 gacgcaaccg ttgacttttt cccctctgat atattccata cgccaaggcg caaaaattgt
     3901 ttccatattt gaaactcgcc gtttttttaa atttatattt ttattatcct acccgttatc
     3961 tgtttgtcaa tttctaatat gccgatggtt tttgaagaat tgaaagagct gtccgccgcc
     4021 gaaccgggat taaagaaaag tacgttgttt tctagatgat tggacggcct gtgcgtgtgc
     4081 ccgtaaacga tacaatggac attcttgaac cgggcggcta tcttttcttc cagcccccag
     4141 ggtgatcccc agccatgaat cagtaaaatt ttaaatccct taatttcaaa aagcaaatga
     4201 tccggatatg ttttcctcac ggaagaacta tccatgttac cgcagacggc tttgacgtcc
     4261 ttatctccaa acatctttaa aacattcaaa tccaccatat cgccggcatg caaaatcatg
     4321 tccgcatcgt caaaatagcc ggcaatacgt ttttgcatct tcggatcata atcatcaaga
     4381 tgcgtatcgg atatgactcc tatttttatt tttttgcctt ccataacgta aaaaccatgg
     4441 cgtcatttaa ctgcttgcgg caaaaaaaac aagctttaaa attagtgaaa cacgccaaga
     4501 atgatattca atattcagga atgaagcgaa ctgaaacact ttggttgaat tatcctgtgt
     4561 gctatccaaa tatgatttca ttaaaggaat aaagctttaa tttgacatta ccggcaagta
     4621 aagttaatat ttcgatcatg aacaatctgg aaaaaactct gaacaaaata gctgaaaata
     4681 tcctgcattt tgacgaggct tcgctgacct cgctctgggc tcaatataaa aataaggtcg
     4741 aaaacttttc gccttccgcc gaatgggaaa aagcggttat catcttctcg attatcaatg
     4801 ccgtgagagc taaaaacgca atttttaacg ataatttatt gcataaaaaa tcttctgcaa
     4861 aagcggaaaa accgcaaaaa aagataaaaa caaaatctta tctgacactt gttaaataat
     4921 ggataaatca tcggatttaa aaagaattat cgatctgaga aaaacgatcg aataccataa
     4981 caggcgttat tatcaacagg attcgcctga aatttccgat gccgaatacg atcggttgat
     5041 gcgtgagctt caggacctgg aaaatcgtta ccctgaggca ttttcaccgt cttctccctc
     5101 tcaaagagtc ggcgcagccc ctttgagtaa atttgccccc ttccatcatc catcgccgat
     5161 gttcagtctg gccaacgctt tttccgaagg cgaaattatc gaatttgacc agcgccttaa
     5221 aagactggcc ggagttaacg atataggcta tatcgccgaa ccgaagctgg atggcctcgc
     5281 ggttaatttg atctacgaaa acggcgtttt caagcaggga gcgacacgcg gtgacggtac
     5341 gaccggcgaa gatgtttcgc aaaatctcaa aacgatctct tccctgccct tgtttatgaa
     5401 aaaaacatcg gaatatcagc ttccttcttt cattgaaatt cgcggcgaag tttatattga
     5461 aaaagacccc tttgaaaaac tcaatcagcg ccgtacccaa gacggtgagg aaccctttgc
     5521 caatccccgc aatgcggccg ccggatcgct gcgtcagctt gattcaagaa ttacggcacg
     5581 ccgccccctc aatatcttcc tgtatggcat cggcgaggca aagggcattg atttttcaac
     5641 gcactgggaa gcactgcaaa tccttgacgg ctgggggttc cctgtgaatc gcgtgattga
     5701 aaaagcatcg aatatcgaag cctgcattca ttattttgag cgcattgccg ctcaacggga
     5761 aaaactgccc tatgagatcg acggcgttgt tctcaaggtt gatgacctat ctttacaaaa
     5821 gatactcggc aatgtctcgc gcaatccgcg ctgggcgctc gcctgcaaat ttccggcggc
     5881 acaggctatg acggtggtca aagacatcat cgtcggtgtc ggacgcatgg gaactctgac
     5941 acccgtcgcc attatggagc cggtcaatgt cggcggtgtc atggtcagcc gcgccactat
     6001 gcataatgaa gatgaggtcc ttaaaaaaga cgtgcgcgtc ggagataccg tggttattca
     6061 acgcgccgga gatgtcattc ccgaagttgt ccggattgtt ccggagaaaa gatcctccga
     6121 tacgaaacaa tttaagatgc ccgcccactg ccccgaatgc ggctccgaag tggtccggta
     6181 tgaaggagaa gtggcccacc gttgcgtgaa catatcctgc ccggcccagt taaaagaaca
     6241 tatccgccat ttcgcgtctc gcggcgcgat ggatattgaa gggctcgggg aaaaagtttc
     6301 tgcgcagctt tttgacgcca agctgattgc cgatcctgcg gatctttatt ttctgactaa
     6361 agaacagctc gtcgatcttg accggcaggc ggaaaaatca gctcaaaatt taatagaagc
     6421 catcgccgga tcgaaaaatc cttctctgga taaattcatc tatgcattgg gtatccgcca
     6481 cgtcggagaa cgcaccgcca agcttctggc aaatcattac ggcagcataa agaatcttat
     6541 ttcgacaacc caggacgaat taacggccat ccatgaaatc ggcccggaaa tagccgaaag
     6601 catcgttgat ttttttcatg aacaaaaaaa tatcaatgtc ctgaataaat ttttcaaggc
     6661 cggcataaat ccgcagaaaa aagaagttca gacgagcgca cctttgcagg ggaaatcgtt
     6721 tgtttttacc ggtaccatgg agaacatggg cagaaacgaa gcccaaacga ttgttgaaaa
     6781 tctgggaggc acaattcatt ccagtgtaac gaagaaaacg acctatgtcg tcgccgggag
     6841 cgatcccggt tcaaaactgg acaaggcaag atcttccgga attcaaatca tcagcgaaaa
     6901 tgaattttta aaactgatag gcaggtaaca ttgaaacaaa ttcatgttca tgtcagaggc
     6961 agagttcagg gagttttttt ccgggcggcc acccaggaaa cggccagggg gttgaatctc
     7021 accggctggg tgcgcaaccg gtcagacgga agcgtggaag ccgtctttga aggtgaagac
     7081 gatcacgtgg acataatgct ggcgtggtgc cgccaggggc cgccgaatgc gcgcgtggaa
     7141 gaagttctta ttcaggaaga accctgctcc ggcctgtttt tggatttcga cattaaatac
     7201 tgaacgcatc cttggacaaa cttgctcccg gcgtatcaat cacaattcga tccggtcgtg
     7261 cagaaccgcg gattgatgac acaagatttt ccttattgcg caggagctat tgaagattga
     7321 tttcgttttt cttctcgaac acactgacac agttttttcc cctgtgcttt gactcataca
     7381 gggcggcatc cgccgcgcga atcagttctt ccttgatttc agaatgatcg ggatacgtcg
     7441 acacgcccag actgatcgtg acatgcaatt tctgatggtc atgagggatt tctattgccg
     7501 caacgccggc ccggattctc tcggccagaa tcacggcctg ttttgaatcg gtatccggca
     7561 gaatcatgac aaactcttcc ccgccgtaac gggccgccac atcttcggaa cgcgatgctt
     7621 tccttgccgc cgcagcaaca tctttcaaaa ccctgtcacc cagctgatgc ccgtaggtgt
     7681 cgttgaaaga tttgaaattg tcaatatcca tcatcacgag tgaaaacttc ctgtcctgac
     7741 ggcgcgaccg ttttatttcc tgatcgagca gaagctgaaa atagcgatgg acatacaact
     7801 tggtcatacc gtcggtcgtg gccagttcga aaagcctcgc gttttcgatg gatatggcgg
     7861 cctgaacgga aaaacttcgg agcacttcca gtcgttccgg cgtaaaggcg ttttcactaa
     7921 tattgttttc catgtaaagg atgccggata ttttgccttt gctcataatc ggcgtgcaaa
     7981 gaatggattt taaacgcacg cggataacgt acgggtcgtt ggtgaaaaga ccttccttta
     8041 cggcattgcc cagaacaata tcttcgccgc tgtgatagac ataattgaca atggagtgac
     8101 agatatcggg gcaatctttc aggggcaggg attgcatcac ctggctctca cctttgtcta
     8161 catcctgact ggcttcaatg gtcagatcgc catccgattc caaaagcaaa taaccgcgtt
     8221 gcgctccggc attcgtaatg gactcatgca tgattttctg cagcagacgg tcgagcatga
     8281 tttcactggc aatgatctgg gagactctca tggctgttga cagatcaagc atttttgtgc
     8341 tgccggtggt ttcggttgca ccgctgatgg tcatcgatcc tgaaggaccg ctcaccgtga
     8401 cggttcccgt cccccattgt ttctgttcca gtgggacaag cgacggatat ttttcctcca
     8461 gatctctggc tttctccgaa gcgccccagc ccaggtagcc tttatgggct ttctgcatga
     8521 aaaatcccgc ttcatccctg catctgtgat caagatacaa aagcgccaga cgttcgcagg
     8581 caatggcctc ttcctgcctg tatccatttt cccgggcgcc ttcaatcgct ttatgataga
     8641 gtttctgcgc ctcccggaag cgccctttgt ttttcatcat ttccgccgcc acaagatcat
     8701 atttatgctg aaaattttcc ggacaagctt tggcccatat gagcattttc atctgattag
     8761 ccctcaacga cattttatac cgggcttttg tcatcggatc cgcctcggga taagatgccg
     8821 tcatagtcaa tgacgaataa agatagaatt ccgggatgtg aagattcccc agcgccgtct
     8881 ttttttgaac aatccttcgc atctcgggaa gaagatccct gcacgccgaa taattgccga
     8941 acagataatt gattctcaac cggaccaacg agaagtaaaa agtgcccagc aaattatttt
     9001 cagacttgta ataaccggcc tgctcctttg catcaaagcc gtccccgttc aaacttcctc
     9061 tgctttcggt ccggcctttc agacacaggc acatgcgcgt attttccatg tagttgcgcg
     9121 cgataaaagg atctttgccg ccgagttgaa aatccctgta ttttccgtat tcttccagaa
     9181 tatcgtcgag gttgtccccc agcatgattc gggtcatgcc gatcagatta acattatgac
     9241 cgctgaaaat aagatcgccg ttttcaatgc cgtttttata agcgtttctg aaataatcaa
     9301 ggtctttgcg ggcgtgtttc ttccagtgct ggataaacat cggaaatgcg aattctatcc
     9361 tgcatcgatt ctttgtatcg gcgatttttt cgttggtttt taatgccgct accccgaacc
     9421 ggtatccgtg ctgatagaag cccagagagg atcccacaat agaccccagg gcgatatagg
     9481 caaacggcga atattcaaaa ttgatctcgt agtccagcag cctgtaaaca ccgttgatgg
     9541 cgataaaagc aaacagattg ggattagcgt aaaaggccac agttccggta tgaatggcca
     9601 gatacgcgta cgaaagcatt tcccagaggt tgatttccgg agatgccagt gattttccgg
     9661 gatgcaccct cagatactcc gcggccatct gttttgcttc atccaacgta ttgggcatgg
     9721 gaatgtattg caaatcaatc aaatcttcta ttttccgacg accgaacttt aagcggagtt
     9781 tcagcagttc ccagcccacc cggacatcgc tgacgttatc cggcagatgg atgccgagca
     9841 ttttcattcc ttccatgccg acatgcagtg ccgcatcgta gtttccctgt gttgtatata
     9901 gaatgatcat cagcgtgtaa acattggcct tgtcaatatt cgaccgggca ttattcacaa
     9961 ccgcatgaaa tattttttcg gcttcgctga aattgaggtt gagatattca cattcaatgg
    10021 cttccttgta tatggcatag gtcagttcgt aatggctttt ccaggcgtat tcgggcaaaa
    10081 gccccatggc cgttttcaaa taaaatgaag cagaaccgta cgctgtggat tttttcgatt
    10141 tctgcgccgc ctgcagattc aatctagcca gcatgatttc ttcatctgtt ccgctgacgc
    10201 accggatgcc tctgttgagc tgatcgacaa tatagaatat tttttcatga agattttttt
    10261 catcggtttt tttgagaaca ttgccgccga tgcgatagtg catcgcgctt ttttcctcct
    10321 cggaaatcat ggaataggcg gcttcctgaa tccggtcatg catgaattta tacatatttc
    10381 cgtgaagact gatcatatct tcctggattg ccgaagtcag atcaaacagt gtttcgtcta
    10441 tggattttcc ggaaacaacc gacaacaact ccagatcaaa tcggttgccg atacaggcgc
    10501 agatttttaa tatttcccgg gtgttcttcg gaaaatgcga tattttttcc gccatgaatt
    10561 caatcacgtt ttcggtaacc tgcatctggc gtattttgtc gatatcccat tcccatccgg
    10621 tggaaggatt cagttggagc aggcgattat cgtaaatatt ttttagaaac tgaatgacga
    10681 aaaagggatt gccggcagtt ttcctgttga tgagttcggc aagcggcatt gatttttccg
    10741 catcgcacaa cagaacattc ataatgatca tgttgatgcc ggtcagattc agaggtccca
    10801 ggacaatgct gtttattttc ttgccttttt tgcggatatg atccagcgcg agcgtcagcg
    10861 gatgcgcatc gttcgtttca ttatcgcgat acgccccgat tagaaacann nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttttttcttc gctttcagaa atgatttgac
    11041 ggatgagtcc ctggaatgac tggatgatgg cgctgtaggg aacatccttg cgaaactggt
    11101 catatttgcc gaaaataaaa tacccttttc tggcaataat cggtttgtaa atctcattga
    11161 ccagcgccga ttttccgatg cccggctggc cggtcaccag catgaactcg cttaatccct
    11221 tgcaggttct ttcaaagcac ctcatcagtt caatccgttc attgtcacga tccacaaggg
    11281 tgcgcggaat attaaaccgg atggaaatat ctttggtggc aatcggaaaa tcgtcaacct
    11341 gtcctttctg gttgagttgc ctgaggcatt catccagatc cgccatgatt cccagacaat
    11401 tctgatatct ctcctcggga ttcttcgaca acattcttaa tataatggaa gacagcaccg
    11461 gcggtatgga cgggtttaga tggtccggcg gcaacggctg tctcgcgata tgcgaatgaa
    11521 tcaattccat aggatctttg gatttaaacg gaacctcgcc ggtcagcatt tcataaaagg
    11581 tgacacccag cgaatagatg tccgttcgat aatccacgcc ccggttcatc ctgcccgtct
    11641 gctccggcga catgtaggac agggttcctt cgatcacgtc cggattgtac agttcgtcat
    11701 tggcatgagt cagaacagcg gaaataccga agtccgcaac tttgacctca tccctttccg
    11761 gattaattaa aatattatcg ggcttgatat ccagatgaat aatattgttt ttatgaatga
    11821 gccccagggt cccggagacc ttggaggaaa tccgcagtaa tgattgcagg tcgagttctt
    11881 tatcctttaa agaatccttc agggaaatgc ccccgaaatc ttcttcaata atggcatact
    11941 ggttatcata gtccaccaga tcaaatattt taacaatgtt atcgacatca agagatttga
    12001 cgagattata ttcctgtttg aaacgtgcaa tttctgatga cgtcggatta atggttttta
    12061 gaagctttat gattaagggc aaaccttctg tctctttata gcctcgaaaa atgacagaat
    12121 ttctggtttc gtgtatcttt tcactcagga tataattggc aaatttctcc ataaagttca
    12181 gacgaattta ataaaatggt ttgtattcag gaaataattg tttaatttat aacacttttc
    12241 tgtcttttga tcaagcatta cctatcatta tattagaagg atcttcagcc ctccacggta
    12301 aatgtatagc gcgcagtaat cctgtaacga ccgggactgt acctgtaaaa cgcccgcgct
    12361 tcgtatcgtc ccggtcccgg agaacgaaac gtcaagacac cctgtccacg ccggggaatg
    12421 tattggtaat ctcccgcatc ggtgtttctt gagccggcag gcacgatgca tatccaatcc
    12481 ctggaatgtc cgggcgcgtt ataataatgt acctgaattt tctcgccgct tcgatacgtg
    12541 tccctgtcag tctggataat ttgcttagat gagtctgcct ctgttttgct ttttccaaaa
    12601 atactactgc cagcctttcc cacttcgtct gaatcatgac tttccagacc ggcttgtgat
    12661 gttgtctttg aagaatcgcc ggataaagtg aaataagcga tcagtccaat agccatgatc
    12721 agcaaaatag caaagattgg ccccaggact ttgatgaaag gaattttatt tatcaaaccc
    12781 gtcgtttctg tttttgattt gggcttaaaa atattcaatt taaaattcca gccgtgtttg
    12841 gcggcaatat ctttcacaac atccttgagc tttccgtatt caaaaccata cttaccgccg
    12901 aacaataatt gtccatgaat tttctttccg acatcgattc ccttttgaac ggtcttttct
    12961 gcgaaaacgc ctgccgtcat tccggcggaa ctttctacca tcttttccca gaacgttatt
    13021 tgtttcgtta gcgggtcaag tatcgctaaa gcggtgtatt ttatcttcac gtgttgaacc
    13081 gcagatcgag ctcccgtttc ggaagaaaat tcaaactgcc ccgcagagga ttcccggaca
    13141 gacaaaaatt cgttgtttaa cgccaaaagc tgtgttgcca cgggtgagga ttccgacgct
    13201 tgggctttcg gcccttccag acggcttcca cagtcgctgc agaaggcgtc ttcaggttta
    13261 ttggatgttc cacaggaagg gcattttttt acgttggaat cttctgcaag acgttgtccg
    13321 caattcgtgc aaaaaatggc atttgcttcc tgaacctctc cgcaggcgga acattttttc
    13381 attttctcaa ctcctcatca tcttaataat attgctctca atggtatttt cctttatgaa
    13441 actgtttcat aatacatttt tttaatacta catccggcta tatcaactca aattcaaaag
    13501 tgctgacgac acgtatcttt ttgacaggcg tgcttgtcgg actgtatgga gcggactcat
    13561 cattgccgtc actgccgaaa atctggatat tcccctgatt ggccgaacgg attttaccga
    13621 cacgcgctcc cgaatcggag gcaaattgct gcgctgtcat acgcgcattt tttgtcgcct
    13681 cggcaagaag cggcggcctc aattcattga atttcgtgat tacgtagcgg ggattagccg
    13741 ttccttcgtc ctgaccatcc agaacaacgc cggatttcag caattcctcg gtttcaccca
    13801 aagctttaat gacgagatcg atattcgagg ttgttacaac cagagagctg gttacaacgt
    13861 aacgatatcg ttcggttgcc tgactttgat catactcccg cgccaattta tcgatggtgc
    13921 gggtcggttg acggctaatt tcctcatcct taaatccctg ttttttgaga aaggcaattc
    13981 cggcatccct gtcggcgctg attttggttt gcgcatcttt gatatcctcg ctcgcccttc
    14041 ggaaacgcaa tacccagatc gactgatcgg cctttaccac tttttccaca agtcctttca
    14101 ccgtgacggt ccgcgtatcc gatttaaaac gcgtgccgcc tttgccgatg aagtatcctc
    14161 cgatcg
//
LOCUS       DAONQZ010000058        14116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4803_length_14116_cov_15.510561, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000058 DAONQZ010000000
VERSION     DAONQZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_14116_cov_15.510561"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="bamD"
                     /locus_tag="PLR13_08900"
     CDS             complement(<1..720)
                     /gene="bamD"
                     /locus_tag="PLR13_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQC19497.1"
                     /translation="MHAKKFVKTAPLKSVLTLLLLSLILGGCSSFSLFGKRDLPQASP
                     EGLYATAAEEYKDGNYKKAREYFSRVKEEYPLHELSVLAEIGIADSLYSNKDYEEAKD
                     AYSDFISLHPINENVPYAVYQTGMCHYHRMEAIDRDQTETINARKEWEKLIARYPESK
                     FSIMAEKMLKEVKQRLAEREFYIGNFYLNHKKYKAALARFEKIAAEYSNVGLDYKIEN
                     YIKEAKTGVDEEEKEKLREDEA"
     gene            complement(752..1081)
                     /gene="trxA"
                     /locus_tag="PLR13_08905"
     CDS             complement(752..1081)
                     /gene="trxA"
                     /locus_tag="PLR13_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQC19498.1"
                     /translation="MSSELLAAATDSNFENEVLKSDKPVLVDFWAPWCGPCKAIGPIV
                     EEIAGQYKDSVKIMKLNVDDNQKMASHFGVLSIPTLILFKEGKVLEKIVGLVPKARLE
                     DLIKKAL"
     gene            1226..1936
                     /locus_tag="PLR13_08910"
     CDS             1226..1936
                     /locus_tag="PLR13_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HQC19499.1"
                     /translation="MGMLGIISTRLVLEQSELLKKSSAKPMENGFGKTTVFTTDKTAL
                     ILRHGMDPDNYILPHLINHPANLQALKDIGVSEIVSINSTGSLKKEIKPGMIVVPDDF
                     LTLTPSPTIHVNRPVHIIPSLNEGIRKKLIRAALKNDFEVVQQGTYWQTQGPRLETKA
                     EISTMTRFADIVGMTMANEAVIAQEMDIPYACACSVDNFGNGLMAEPLSMEEIISHTR
                     KNAQRMIQLLDRYLQMFF"
     gene            complement(1938..2372)
                     /locus_tag="PLR13_08915"
     CDS             complement(1938..2372)
                     /locus_tag="PLR13_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19500.1"
                     /translation="MTHEDKGKYFKKHPEGTRIDDELKDEIISQVKNNNITCKKAEEI
                     ASALGCSLAETGRAIDILNINITKCQLGLFGYGDTKKIVQPVKEVAPELKESIMSALE
                     NERLSCAAVWGIAKKFNMPRMKVCAACEALDIKVKPCQLGAF"
     gene            complement(2397..2828)
                     /locus_tag="PLR13_08920"
     CDS             complement(2397..2828)
                     /locus_tag="PLR13_08920"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQC19501.1"
                     /translation="MVAYQLSIFAENKPGKLARVTGVLAKEKINIRATTIATADTFGV
                     INLIVDDPRRAEKALSDAGMTVHLREVLAVLISDQPGALDSLTQLFQKEGVNVNNAYG
                     FVLEGSKKAVFVVDVDQIRKTEKLLEHEGFKTLDTQSLSSV"
     gene            complement(2852..4489)
                     /locus_tag="PLR13_08925"
     CDS             complement(2852..4489)
                     /locus_tag="PLR13_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQC19502.1"
                     /translation="MPITEILARNAAQYGEEISLVEINPQIKEIKANWKDYELVEPNP
                     EKRYRREMTWREFDDKANRFANFLLDRGLEKGRKVGILLMNCLEWLPIYFGVLKTGAL
                     AVPLNYRYTEDEIKYCLDLAECDVLVFGPEFTERIDAIKDTIPNVKIRLFVGQDCPAF
                     AESYYALTACCSSANPKISLFDGDDAAIYFSSGTTGFPKAILHSHSALMMSCEVEMNH
                     HGQTRTDNFLCIPPLYHTGAKMHWFGSFLAGSRAVLLRGVKPEWIFKAVSEEQITIVW
                     LLVPWAQDILDAVERGDIKLENYKLDQWRLMHIGAQPVPPSLIKRWKKYFPVHEYDTN
                     YGLSESIGPGCVHLGTENIHKVGAIGVAGYKWDVQIVDEKGTPVKQGEVGELCVKGAG
                     VMKCYYRNPEATAEVLKNGWLYTGDMARMDEDGFIYLVDRKKDVIITGGENIYPVQIE
                     DFLRTHDAIKDVAVIGLPDQRLGEIVAAVIEFKPGFAATESEIAEFCNALPRYKRPRR
                     IIFEKVPRNPTGKIEKPKLREKYFGIGSLVEAQTENL"
     gene            complement(4682..5239)
                     /locus_tag="PLR13_08930"
     CDS             complement(4682..5239)
                     /locus_tag="PLR13_08930"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQC19503.1"
                     /translation="MLSNKIIRKTPFGPVCIIWSVSKDHPRIIQIMLSRPGLSAEDRV
                     SQLFPESKISSCAEIDHVASSIRAYLEGEDITFHLNLVDLSPFSRFQQSVLLAQHAIP
                     RGKVSTYGLIAVHVGAPGGARAVGNVMAINPYPLIIPCHRTVLSDCRLGGFQSGITMK
                     KALLTQEGVMFDQNGRVVCRQLHYD"
     gene            complement(5246..5626)
                     /locus_tag="PLR13_08935"
     CDS             complement(5246..5626)
                     /locus_tag="PLR13_08935"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HQC19504.1"
                     /translation="MGNKAKYKISSSQIDGILEIVLTGEVSKEDVETLQNEIFSIIIN
                     TGAEALLVDVRAIKGRFGYLEAYSRVRHYPTDTPRIHTAIVDNAENADYESFHETTAY
                     NAGLSYKWFTDIEKARSWLKDKIK"
     gene            complement(5691..6431)
                     /locus_tag="PLR13_08940"
     CDS             complement(5691..6431)
                     /locus_tag="PLR13_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19505.1"
                     /translation="MSRILAAPLKSNSHAFMKSSFIPAFIAFGLMSYLAVVQQWSLQE
                     FCWCVWLAGLFYSWTCVMTAAIQMMLTARAQKDYYHANIPLMKKIPPALFALGMIPVA
                     LLIGFVGLYLYTWLFSFYGLFLSVFAEMPPVDLFGRNGFINSDFFTPVTYLTDQYWPM
                     ILATLAANADGLLRATPWKRIIFPFKQNEILRIHVMTLLMPFLAMMTWEFFRDAYQQL
                     TIILLIGIFYLLPKRRFKQENMANNPLK"
     gene            complement(6428..6826)
                     /locus_tag="PLR13_08945"
     CDS             complement(6428..6826)
                     /locus_tag="PLR13_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19506.1"
                     /translation="MRDEIIVEYNQEGYVHAKHFGKNNYDLSLEVWRRIKALCKEHNC
                     FKILGENHTTEELSTMDAFNHLNILEEVGITFQYRIAWVDQGTPTAKGLEFVETVVVK
                     NRGLVNGKLFADLEEARRWLLGKDNPQRNS"
     gene            7142..7687
                     /locus_tag="PLR13_08950"
     CDS             7142..7687
                     /locus_tag="PLR13_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQC19507.1"
                     /translation="MGKGIVDRLKKEYPLEDHWVSFELHPETPPAGVLLAERFKDYDL
                     SSFYEQLCQRGKEVSVVFGNQKILSNSRFALMASEYARDLDRYDSFHENMFHAYFTEG
                     LDIGKTDVIAAVAKKSGLDEKGTRNAIHDDRYASRLNEARKEGKSLGLTGIPLFIINN
                     KYQITGAQPIETFRELLKKIT"
     gene            7775..8245
                     /locus_tag="PLR13_08955"
     CDS             7775..8245
                     /locus_tag="PLR13_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7904..7906,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HQC19508.1"
                     /translation="MARVELNVQAPDFTLKDFNGTIVSLSDFQNKKNVMLVFNRGFIU
                     PFCRVHMAQLRQDYEKFVKLDTEILVAGPEDAEAFKKYWVKEKLPFVGLPDPEHHVLK
                     LYGQEVKIFKLGRMPAQIIIDKSGKVRFVHYGHSMMDIPENKELLELLATQIEK"
     gene            8535..8939
                     /locus_tag="PLR13_08960"
     CDS             8535..8939
                     /locus_tag="PLR13_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQC19509.1"
                     /translation="MSELLAYLSPAWCDEAFKRLQTELTPDKMNKVSTSMSNIYKNCP
                     GGKEMFLFIECKEGNITRVETGVGDPPKAEFLIIGDYETFSRISRAELGSQKALMTGK
                     IKLKGNLAKALKLAAVSDRLNKVLAQIPTQYG"
     gene            8951..10174
                     /locus_tag="PLR13_08965"
     CDS             8951..10174
                     /locus_tag="PLR13_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQC19510.1"
                     /translation="MPRIFDPNDKYYIDNPGRVKAIQAEMHKRGIDVYLGSRIRTMSF
                     VLDAFCPWRSYLIIPSEGQPAFFTFVIDALRVGDETWLDSDHVRGYGPMGGMDQVSAI
                     TDYIKDELGIKKGRLGYEDGVSTYTAEGNLSHWEYTRLRDALPDFEWVNAHDIIDGLS
                     LIKDKGTIERFRTASLIVDEGHKAARAALENGGYRGMTETVIAGIAALAMRKAGSVSE
                     WNFAGLNEISSGYRTGLGACTPPTTKEIKAGEPLMLDFHAMFKLALGDHSHNYLIDPA
                     TKRQRWHADNFVSIIRKVIDNYRAGTTPGKLAAMMMDFAESRGCADFILPGCEHGIGL
                     FGDEWRIGSIVNPDLPYWTDQDHVYQENEMVVCAMQYAAPEEDIGFRYENDIVITKDG
                     CDVMSKYPLGIEEIS"
     gene            10415..10978
                     /locus_tag="PLR13_08970"
     CDS             10415..10978
                     /locus_tag="PLR13_08970"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HQC19511.1"
                     /translation="MFKKLLLLCSVVLILTSVGYAKEVGGINMPDTLAAGTDTLILNG
                     AGIRTKFFIKAYVTGLYLKKKNSDANAILNADEPMAVRIHIISKLITSDKMKDATMEG
                     FQNSTNGNIAPYKDKIDTFISVFADNIKINDVYDLVYTPAEGTQVYKNKQLKTTTKGL
                     DFKKVLFGIWLCNKPADKALKSQMLGK"
     gene            11158..11955
                     /locus_tag="PLR13_08975"
     CDS             11158..11955
                     /locus_tag="PLR13_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQC19512.1"
                     /translation="MFQWFINRRRSKLTAAPFPDAWEDILERNMGHYRLLHDAERAHL
                     RSLIQVFIAEKHWEGAGGLVLNDEIRVTIAAHACLLLLRLSHNYYRNVETVIVYPSTL
                     VAPRRKPGFFEVSLEPLEENYPIVGEAFQQGPVIIVWDAALQGGRQSESGHNVVYHEF
                     AHKLDMLDGAADGTPPLKDRAKYRDWIKTFSHEYLRLRRDTEKGRQTFLDAYGATNEA
                     EFFAVATEHFFEQPLIMEKNAPELYRRLREYYGQDPASRFRQNNCAV"
     gene            complement(12028..13743)
                     /locus_tag="PLR13_08980"
     CDS             complement(12028..13743)
                     /locus_tag="PLR13_08980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19513.1"
                     /translation="MNYLVQPKKYQSLMTDDGSRAIMDKTVAFFEKMGKERLLDDYNK
                     KVWYREFVDFIGREQIFAKLLTPSQYGGGDPDCRWDTARNSEYSELLAFYGLGYWYCF
                     QVSILGLGPIWMSPNEKAKKKAAELLKKGAIFAFGLSERTHGADIYSTETTLTPADNG
                     TWVANGEKYYIGNGNEAEMVSTLGKVKDGTDDYVFFVTNYKHKAYELKKNVISHQEYV
                     SNFALHDYPITEDEILMRGPHAWDSALNTVNIGKFNIGPASIGVVEHCFYEAITHASN
                     RILYGMKVTDMPHVRKNFMDAWLRLVAMKLYQRRSTDYFRNANDKDRRYLLYNPTSKM
                     KVTLQSEDVLNLLWEVIAAKGFEKDTYFHMAAADIRGPSKLEGTVHVNVQLIRKFMKN
                     YFFNPVSYAPVAPDFSAKDDLFLFNQGPTKGLGSVQFHDYKPVFEAGRKLPNVEIFIR
                     QINIFKEMLEKAGPDKAQDLDPSWSLPVGEMFSIVVYGQLILEQAAFDKVDDDILNQI
                     FDFMVRDFARFALQIYGMHNTKEDQRAYCKDIMLIKGQGDDAQYDRVWQKYVAVLNGE
                     YAMSE"
BASE COUNT         3669 a         3552 c         3218 g         3677 t
ORIGIN      
        1 ggcctcatcc tctcttaatt tctctttctc ttcttcgtca acaccggttt ttgcttcctt
       61 gatgtaattc tcgatcttgt aatcgagtcc gacgttgctg tattcggcgg ctatcttttc
      121 aaaacgcgct agggctgctt tatatttttt gtgattgagg taaaaattcc cgatatagaa
      181 ttcgcgttcg gccagcctct gtttcacttc tttcagcatc ttttccgcca taatgctgaa
      241 tttactctcc ggatagcggg caattaactt ttcccattct tttctggcat tgattgtttc
      301 cgtctgatcc cgatctattg cttccatacg atgataatga cacattccgg tctgatagac
      361 ggcataagga acattttcat tgatgggatg gagagaaata aaatcgctgt aggcatcttt
      421 tgcctcttca taatccttat tgctgtatag cgaatcggca atgcctattt ccgccagtac
      481 tgacagttcg tgcaggggat attcttcctt gacacgtgaa aaatattccc gtgccttttt
      541 gtaattccca tccttatatt cctcggcagc cgttgcatag agtccttccg ggctagcctg
      601 gggaaggtcg cgtttgccga aaaggctgaa agacgaacaa cctccgagaa ttagtgataa
      661 taacaaaagc gtcaaaacac tttttaatgg cgctgttttt acgaatttct ttgcatgcat
      721 caacgttaac ctctcggact gtattttcgg cttacaatgc ttttttgatt aaatcttcaa
      781 gtctggcttt cggcaccagg ccgacgatct tttccagaac tttaccctct ttaaagagaa
      841 tcagcgtggg aatgctgaga acgccgaaat gcgacgccat tttctgatta tcatcgacgt
      901 tcagcttcat tattttcaca ctgtctttat actgtccggc tatttcctca acaatcggac
      961 cgatggcttt acagggaccg caccacggtg cccagaaatc aacaagcacg ggtttgtcgc
     1021 ttttcaaaac ttcattttca aagttactgt cggtggcagc agccagtagt tcactgctca
     1081 ttataaagcc ctccttagat ttttagagtg aatctttatg tcacaataag ccccataaag
     1141 tcaaatgcaa tctggcaaca gatccgcaac gacctttgac ggtttcccgt taatttgcta
     1201 tataatcggg ctcaaaggag aatctatggg catgctgggt attatttcta caaggctggt
     1261 tctggaacaa agtgaattgc tgaaaaaaag ttccgctaag ccgatggaaa acgggttcgg
     1321 taaaacgact gtgttcacga cggataaaac agcgctgatt ctgagacacg gcatggatcc
     1381 ggacaactat attttacctc atttgatcaa tcatccggcc aatcttcagg cgctgaaaga
     1441 tatcggggtt tccgaaattg taagcatcaa ttccaccgga tcgctaaaaa aagaaatcaa
     1501 accggggatg atcgtcgttc cggatgattt tctgactctg actccctcgc cgaccattca
     1561 cgtgaacaga cccgtccaca ttatcccgtc attaaatgaa ggcattagga aaaagcttat
     1621 ccgagccgct ttgaaaaatg atttcgaggt ggtgcagcaa ggaacctact ggcagacaca
     1681 gggccctcgg ctggaaacaa aagccgaaat cagcacgatg acccgctttg ccgacattgt
     1741 cggcatgacc atggccaatg aagctgtcat tgctcaggaa atggacattc cctatgcctg
     1801 cgcctgttcc gtcgataact tcggaaacgg cctgatggct gaacctttaa gcatggaaga
     1861 aatcatcagc cacacccgca aaaacgcaca gcggatgatt caactgctgg accgttacct
     1921 gcaaatgttt ttttaaatca gaaagcaccc agttgacagg gcttaacctt gatgtccagc
     1981 gcttcgcacg cggcgcatac cttcatccgc ggcatattaa atttcttcgc aatgccccag
     2041 acggccgcgc aggacagcct ttcattttcc agagcggaca tgatgctttc tttcagctcc
     2101 ggcgccacct ccttcaccgg ttgaacgatt tttttggtat caccgtaacc gaaaagtccc
     2161 agttggcatt tggtaatatt aatattcaga atatctatcg ctctgcccgt ttctgccaga
     2221 ctgcatccca gtgccgaggc tatctcttcc gcctttttgc aggtgatatt attattttta
     2281 acctgactga tgatctcatc tttcaattca tcatcgattc gggtcccttc cggatgtttt
     2341 ttaaaatatt tgcctttatc ctcgtgcgtc atgttgcctc ctccttgttg aatttttcat
     2401 acagaagaaa gtgactgagt gtccagtgtc ttaaaacctt catgctccaa aagtttctcc
     2461 gttttccgta tctgatcgac atcaacgaca aatacggctt tcttcgaccc ctccagcaca
     2521 aagccgtagg cattattgac attgacccct tccttttgaa atagttgagt cagactgtcc
     2581 agcgcgccgg gttgatcgga aatcaaaacc gccaacactt cccgcaaatg caccgtcatg
     2641 cccgcgtcgg atagtgcttt ctcggcacgc cggggatcgt cgacgatcag gttgatcacg
     2701 ccaaacgtat ccgccgttgc tatggttgtg gcgcggatat tgattttttc ttttgccaga
     2761 acaccggtta cccgggccag tttcccgggt ttgttttccg cgaaaataga aagctgataa
     2821 gcaaccattc ccttctctcc ttcctgttga gttagaggtt ctccgtctgc gcctccacaa
     2881 ggcttccaat tccgaaatac ttttcacgga gtttcggctt ttcgattttt cccgtgggat
     2941 tgcgaggcac cttttcaaaa ataatcctgc gcggacgctt atagcgcggc aatgcattac
     3001 agaattccgc aatttcggat tctgtcgcag caaaacccgg tttgaattca atgaccgccg
     3061 caacgatttc ccccaagcgc tgatcgggaa gtccaatgac cgcgacgtcc ttgatggcgt
     3121 catgggtgcg caggaaatcc tcgatctgca cgggataaat attttcgccg ccggtaataa
     3181 tgacatcctt tttgcggtcc accagataaa tgaatccgtc ttcatccatt ctcgccatat
     3241 ctcctgtgta aagccagccg tttttgagaa cttcagcggt ggcttccgga tttctgtaat
     3301 aacatttcat cacacccgcg cctttgacgc aaagttcacc cacttcaccc tgcttcaccg
     3361 gtgtgccttt ttcatcaacg atctgcacgt cccatttata accggctacg ccgatggcgc
     3421 cgaccttatg gatattttca gtgccgagat ggacacaccc cggcccgatg gattccgaaa
     3481 ggccgtaatt ggtgtcgtat tcatgtacgg gaaaatattt tttccagcgt ttaatcaggc
     3541 tgggcggaac cggctgtgcg ccgatgtgca tcagccgcca ctgatcgagc ttgtaatttt
     3601 ccagcttgat gtcaccgcgt tcgacggcgt ccagaatatc ctgcgcccac ggaaccagca
     3661 gccacacgat ggtaatctgt tcttcggaaa ccgccttgaa aatccattcc ggcttcacac
     3721 cgcgcagcag aacggcgcgg cttccggcca ggaagctgcc gaaccagtgc atttttgcgc
     3781 ccgtatgata aagaggcggg atgcagagaa aattgtctgt gcgcgtctgg ccatgatgat
     3841 tcatttccac ttcgcacgac atcatcaggg cgctgtgcga atgaagaatg gcctttggga
     3901 aaccggtcgt gcctgacgag aaataaatgg ccgcatcgtc gccgtcgaaa aggctgattt
     3961 tcggatttgc cgaggaacag caggcggtca gcgcataata actttcagca aacgcgggac
     4021 aatcctgacc tacaaacagc cggatcttga cgttcggaat cgtatccttg atcgcatcga
     4081 tcctttcagt aaattccggt ccgaagacca acacatcgca ttcggccaga tccaggcagt
     4141 atttgatttc atcctcggta taacggtagt tgagcggcac ggccagcgct ccggttttca
     4201 aaacaccgaa ataaatcggc agccactcca gacagttcat cagcagaatc ccgaccttcc
     4261 ggcccttctc caaccctctg tccagcagaa aattggcaaa gcggttggcc ttatcgtcga
     4321 attcgcgcca ggtcatttcc cgtctgtatc gtttttcagg attgggctcg acaagctcat
     4381 agtccttcca gtttgccttg atttctttga tttgcggatt aatttcaaca agacttatct
     4441 cttcgccgta ctgagcggcg ttgcgggcaa gaatttctgt aatgggcatt tctgattcct
     4501 tctaatcttt actgttttgc aggttcttta taggcgactt ttatcctgtc agttcatgat
     4561 caacacgctt atcaggaacc tgttaaacgt aaaatacgat acgaatttca ggaatgtatg
     4621 tcaagaatgt tttggacaaa ataaatgaaa caaaagcgct atttctgatg gctgcccctc
     4681 tttaatcgta atgcaattga cgacaaacca ctcttccatt ctgatcaaac atcacgcctt
     4741 cctgtgtcaa taaagccttt ttcatcgtaa taccgctttg aaaaccaccc aatcgacaat
     4801 cggaaaggac ggttcggtga caaggaatga ttaaaggata tggattgatt gccatcacat
     4861 tgcccaccgc ccgcgctccc cccggcgctc ccacatggac ggcaatgagt ccgtacgtgc
     4921 tgaccttgcc tcgcggaatc gcatgctgcg ccagcaaaac cgactgctgg aatcttgaga
     4981 aaggcgacaa atcaacaaga ttcagatgaa acgttatatc ctccccctcc agataggctc
     5041 tgatggaaga agcgacgtga tcgatttcgg cgcaggacga tatttttgat tcagggaaaa
     5101 gttgcgatac gcgatcttca gccgaaaggc ccggacgtga aagcataatt tgaataatgc
     5161 gcggatgatc tttggacacc gaccagataa tgcagacagg cccgaaagga gtttttctga
     5221 taattttgtt acttaacata atagtttatt ttattttgtc tttgagccag gacctggctt
     5281 tttcaatatc ggtaaaccat ttataagaaa gaccggcatt gtatgccgtg gtttcatgaa
     5341 aactttcgta atcagcattt tcggcattgt ctacaatcgc cgtatgtatt cttggcgtat
     5401 cggttggata gtgtctgacc ctggaataag cctccagata gccaaatcgt ccctttatcg
     5461 ctctaacatc caccagcaaa gcttcagcgc ctgtgtttat gatgatggag aagatttcat
     5521 tttgtaacgt ttcaacatcc tccttgctca cttcgcctgt cagaactatt tccagtattc
     5581 cgtctatttg cgatgatgaa attttatatt tcgctttatt tcccattaca gaactcctta
     5641 aattaaattt aatatccgga atcgacttgc agacgtcact ggcgtttcaa ttatttcagt
     5701 ggattatttg ccatattttc ttgcttaaac ctccttttcg gaagcaggta gaaaatacct
     5761 atgagcaaaa taattgtcaa ctgttgataa gcgtccctga aaaattccca ggtcatcatc
     5821 gccagaaaag gcatcagcag agtcatcaca tggatgcgca ggatttcatt ttgcttgaag
     5881 gggaatatga tccgtttcca gggggttgcg cgcagcaggc catctgcatt tgcggcaagc
     5941 gtcgctagaa tcatcggcca gtactgatct gtcaggtaag tgaccggcgt gaaaaaatcc
     6001 gaattgataa aaccgttcct gccaaaaaga tcgactggcg gcatttcggc aaagacggaa
     6061 agaaataatc cgtaaaaaga aaacagccag gtataaagat aaaggccgac aaatccgatc
     6121 aacaatgcca cgggaatcat tcccagggca aacaacgccg gcgggatttt cttcatcagg
     6181 ggaatattcg cgtggtaata atctttttgt gcccgtgcag tcagcatcat ctgaatcgca
     6241 gccgtcatca cgcatgtcca cgaataaaaa agtccggcca gccacacgca ccagcagaat
     6301 tcctgcaatg accattgctg gacaaccgcc agataggaca tcaagccgaa ggcaatgaag
     6361 gctggaatga aggatgattt catgaaggca tgcgagtttg atttcagcgg cgccgccaaa
     6421 atgcggctca cgaattgcgc tgcggattgt ctttgcccaa cagccagcgc ctggcctctt
     6481 caaggtcagc gaataatttc ccgtttacca gaccgcggtt tttcacgaca accgtttcca
     6541 cgaattcgag tcccttggca gtcggcgtcc cctgatccac ccacgctatg cggtactgaa
     6601 aggttattcc cacttcttcc aggatattga ggtgattgaa agcatccatt gtggagagtt
     6661 cttccgtggt atgattctct cccagaatct tgaagcagtt atgttccttg catagcgcct
     6721 tgatacgcct ccatacctca agcgagaggt cataattgtt tttaccgaaa tgctttgcat
     6781 ggacgtagcc ttcctgatta tattccacga tgatctcgtc gcgcatcgta catcctccct
     6841 caacattcag aatgaatgtg actgaattca tttctctgtg ttgcacatgg aacacaggct
     6901 tccacggtac cgatcaaacc aatccaaaaa ccacagggca tttacagcgc aaattttatc
     6961 gaaggcaaaa catttttttt gaaagttgag cttacgtttt ttcatcggat attgcaatat
     7021 ttctttttta aaaaacgatg taaaattacg ataaatgcga gtatatttaa cgaaaatttc
     7081 gaggcataaa atgacaatcc acctcaaaat atattccgac tatatctgac ccttctgcta
     7141 catcggcaag ggcattgtcg accggctgaa aaaagaatat cccttggagg atcactgggt
     7201 gagcttcgag cttcatccgg aaacgccgcc ggcaggagtg ctgcttgccg aaagattcaa
     7261 ggattatgat ctttcttcat tttatgagca actgtgccaa cgcggcaaag aggtaagtgt
     7321 tgtgtttggc aatcaaaaaa tcctttctaa ttcaagattc gcactaatgg cttccgaata
     7381 tgcccgcgat ctggaccgat acgattcgtt tcatgaaaat atgtttcacg cttattttac
     7441 tgaaggtctc gatattggca aaacggatgt tattgccgct gttgcgaaaa aaagcgggct
     7501 tgacgaaaag ggaacacgca acgccatcca cgatgaccgt tatgcgtcac gcctgaacga
     7561 agccagaaag gaaggaaaat cgcttggcct cacaggcatt cccctcttca tcatcaataa
     7621 caaataccag atcaccggtg ctcagccgat tgaaaccttc cgtgagctat tgaaaaaaat
     7681 tacttaagat atatacgcct atccttacgt taaaaacgaa ttgaaaattg aaaatttcag
     7741 cgtatcattt aaaaatagtt aaggaggtta acttatggcc cgagtggaat taaatgttca
     7801 agcgccggat tttacgttga aggatttcaa tggcaccatc gtttcgcttt ccgattttca
     7861 aaacaaaaag aatgtgatgc tggttttcaa ccgcggtttt atctgaccgt tctgccgcgt
     7921 gcacatggcg cagttgcgcc aggattacga aaagtttgtc aaactggata cggaaattct
     7981 ggttgcagga ccggaagatg ccgaagcttt caaaaaatac tgggtgaaag aaaagctgcc
     8041 ctttgtaggc ctgccagatc ccgaacatca tgtgttgaaa ttgtacggcc aggaggtcaa
     8101 gatattcaag ctgggcagaa tgcccgcgca gatcatcatc gacaaatccg gcaaagtccg
     8161 ttttgtccat tacggccatt cgatgatgga cattccggaa aacaaagagc ttctcgaatt
     8221 gctggcaacg caaatagaaa aataaaaaaa cgggtttgcg ttttcacaac tccaacaacc
     8281 tgaaggctgc ggtcaggtca ccatcagcaa gcatggttat ggcggacgcc tgaggctgtg
     8341 tttgtatgac aagcttttgg tatccttgag gtttattgac attgacttac ctcttctttg
     8401 cccctataat tttatattga tgagaaagtg ctccgtcaac tttttggcga gaagcgaaaa
     8461 acacctgtaa aggggtaccc atccggatgt catgttcatg acgtccggaa tcagacaacg
     8521 caggaggtat cagcatgtca gaattacttg cctacttaag cccggcatgg tgcgacgaag
     8581 catttaagcg acttcagacg gaacttacac cggataaaat gaacaaggtt tccacatcga
     8641 tgtccaacat ttacaagaac tgccccggcg ggaaagaaat gtttttattc atcgaatgca
     8701 aggagggaaa cataacccgt gtggagaccg gagtcggcga cccgcccaag gctgaatttc
     8761 tcatcatcgg cgattatgaa acgttttcca gaatttcccg cgcggaactc ggctctcaga
     8821 aggcgctgat gaccggaaaa atcaaactca aggggaatct ggcgaaggcg ctcaagctcg
     8881 cagctgtatc ggacaggctg aataaagtcc tggcccaaat tccaacccaa tatggataag
     8941 gagagaaatc atgcccagaa tattcgatcc caacgataaa tattatatcg acaaccccgg
     9001 acgcgtcaaa gccattcagg ctgaaatgca caaacgcggc atcgacgttt acctcggttc
     9061 acgcatccgc acgatgagtt tcgttttgga tgccttttgt ccctggcgca gctatttgat
     9121 tatcccgtcc gagggacaac cggctttttt tacatttgtt atcgacgcgc tgcgcgtggg
     9181 cgatgaaacc tggctggaca gcgatcatgt ccgcggctac ggcccgatgg gcggcatgga
     9241 tcaggtgtcc gccatcaccg attacattaa ggacgaactc ggcatcaaaa aaggacggct
     9301 gggatatgag gacggcgttt ccacctatac tgccgaagga aatttgtcgc actgggaata
     9361 tacccgtctc agggatgctc ttccggattt tgaatgggta aacgcgcacg atatcatcga
     9421 cgggctttcc ctgataaaag acaagggaac gattgaacgc ttccgcacgg cgtcactgat
     9481 tgtggatgaa ggccacaaag cggcgcgggc cgcgctcgaa aacggcggct acaggggcat
     9541 gacggaaact gttatcgccg gtattgccgc gctggccatg cgcaaagcgg gcagcgtatc
     9601 ggaatggaat tttgcggggc tcaacgaaat ctccagcggt taccgcaccg gcctgggcgc
     9661 atgcacaccg ccgacaacga aagaaatcaa agcgggcgaa ccgctgatgc tggactttca
     9721 cgccatgttc aagctggcgc ttggcgatca ctcgcacaat tatctcatcg atcccgcaac
     9781 aaaacgtcag cgctggcacg cggataattt tgtctcgatc atccggaaag tcatcgataa
     9841 ttaccgcgcg ggaaccacgc ccggaaaact ggccgccatg atgatggact tcgcggaatc
     9901 ccgcggctgc gctgatttta ttctgcccgg ctgcgaacac ggcatcggtc tgttcggcga
     9961 cgaatggcga atcggctcta ttgtcaatcc cgacctgccc tactggacgg atcaggatca
    10021 tgtttatcag gaaaatgaaa tggtcgtttg cgcgatgcag tacgccgcac cggaagaaga
    10081 tatcggcttc cgttatgaaa atgatattgt catcaccaaa gacggctgtg acgtcatgtc
    10141 gaaatatccg ctcggcattg aagaaatttc ttaaacgtcc tctcgccttc cggcgcttct
    10201 gacggcgccg gaaggcctgt ccattgcccg acaggccgca gtcccggggc aatctcttta
    10261 aaaagatatg gaaataaata attgacagac agaaggcttt gtctctatac tccccgcaca
    10321 gaaagaaagg tcccgaagcg gaaccggaat attttatcct tcagcattca cttggcctta
    10381 agcgggctaa aataaaaaaa gagaggtgtt cattatgttt aagaaattat tgctgttgtg
    10441 ctctgtcgtt cttatcctta catccgtggg ctatgcaaaa gaagtcggcg ggatcaacat
    10501 gccggacact ctcgctgccg gcactgacac gctgatcctg aacggcgccg gcatacgaac
    10561 gaaattcttc atcaaagcgt atgtcaccgg gctgtacctc aagaaaaaaa acagcgatgc
    10621 caacgcgata ctgaacgccg atgaacccat ggccgtcaga atccacatca tttccaaact
    10681 gatcaccagc gataagatga aagacgccac catggaagga ttccagaatt ccaccaacgg
    10741 caatatcgcg ccctataaag acaaaatcga tacgttcatc tctgtgtttg cggacaacat
    10801 caagatcaat gacgtttacg acctcgtata cacccctgca gaaggaacgc aggtatacaa
    10861 aaacaaacag ttgaagacca ccaccaaagg gctggatttc aaaaaagtgc ttttcggcat
    10921 atggctgtgc aacaaaccgg ctgacaaggc attgaaaagt cagatgctgg gcaaataaag
    10981 atcgacatgg tttaaaaaag attgccttct cagggcgaaa agacttccat cgaacagaag
    11041 gcttttcgcc cttgattttt ctttgaatca gtgtgaacct tccatttcag aaacaccaga
    11101 gggatctatt catttgccaa aatatttttt attgacttga agtaaaagga accccatttg
    11161 tttcaatggt tcatcaatcg ccgacgcagc aagcttacgg cagcgccatt tcccgatgcc
    11221 tgggaggaca ttctcgagcg caatatggga cactaccgtc tgctccatga tgcagaacgc
    11281 gctcatctgc gttcgctgat acaggtgttc attgctgaaa aacactggga gggcgcggga
    11341 gggcttgttc tgaacgatga aatccgcgtg accattgcgg ctcacgcctg cctgctcctg
    11401 ctgagattgt cccataacta ttaccgcaat gttgaaaccg tcattgttta cccctccacg
    11461 ttggtagcgc ctcggcgcaa acccggtttt ttcgaagtct cgctggagcc gctggaagaa
    11521 aattatccga ttgtcggaga agcctttcaa caggggccgg tcatcattgt ctgggacgcc
    11581 gcgttgcagg gtggccgtca gtccgaatcg ggacataatg tcgtttatca cgagttcgcc
    11641 cataagctgg atatgcttga cggcgcagcc gacgggaccc cgccgctgaa agaccgcgcc
    11701 aaataccgcg actggatcaa aacattttca cacgaatatc ttcgtctcag acgcgacacc
    11761 gagaaaggca gacagacgtt tctcgacgcc tacggcgcca ccaatgaagc ggaattcttc
    11821 gccgtggcca cggaacattt ctttgaacaa ccgctcatta tggaaaaaaa cgccccggaa
    11881 ctctaccgga gactcaggga atattacggg caggatcccg cctcgcgttt ccgtcaaaac
    11941 aactgcgccg tttgaaaaaa aaagggcgaa gccattgtgc ttcgcccttc aatccaatgt
    12001 ccgaacgtct tcggccgttt gtgcagccta ttcactcatc gcgtattcgc cgttcagaac
    12061 ggcaacgtat ttctgccaga ccctgtcata ctgggcatcg tcgccctggc ctttaatcag
    12121 cataatgtct ttgcagtagg cgcgctggtc ttctttggta ttgtgcatac cgtagatctg
    12181 cagggcaaag cgggcaaagt cgcgcaccat gaaatcgaaa atctgattga ggatatcatc
    12241 atccaccttg tcgaaggcag cctgctctaa aatgagctgt ccgtacacca cgatggagaa
    12301 catttcgccc accggcagtg accaggaggg atccaaatcc tgcgccttgt caggacccgc
    12361 tttttcaagc atttccttaa agatattgat ttgccggatg aaaatctcca cattgggcag
    12421 ctttcttccc gcttcaaaaa ccggcttgta atcgtgaaac tgcacagatc ccaggccttt
    12481 ggtcggtccc tgattgaaca gaaacaaatc atctttagca gagaaatcgg gagcgaccgg
    12541 agcataggac acggggttga agaaataatt tttcatgaat ttgcggatga gttgaacgtt
    12601 cacgtgcacc gttccttcca gtttggacgg cccccggata tcggcggcgg ccatatgaaa
    12661 gtaggtgtct ttttcaaagc ccttggcggc aataacctcc cacaaaagat tcaaaacatc
    12721 ttccgactgc agggtaacct tcattttgct ggtgggattg taaagaagat agcgccggtc
    12781 tttgtcattg gcgttgcgga agtagtctgt ggaacgcctc tgatacagct tcatcgccac
    12841 cagcctgagc caggcatcca taaaattctt acgcacatgc ggcatatccg tcaccttcat
    12901 gccatagagg atgcgattgg acgcgtgggt aatggcctca taaaagcaat gctcgaccac
    12961 gccgatggac gcgggaccga tgttgaattt gccgatgttc acggtattga gcgccgaatc
    13021 ccaggcgtga ggcccgcgca ttaggatctc gtcttcggta atcgggtaat catgaagcgc
    13081 gaaattggaa acgtattcct gatgagaaat aacgttcttc ttcaattcat aggctttgtg
    13141 cttgtaattg gtgacgaaga aaacatagtc gtcggtaccg tccttgacct tgcccaaggt
    13201 ggaaaccatt tccgcttcgt ttccgttgcc gatgtaatat ttctcgccgt tggcaaccca
    13261 ggtgccgtta tcggccggcg tcagcgttgt ttcggttgaa tagatatccg cgccgtgcgt
    13321 ccgctcggac aaaccgaagg caaatatcgc ccctttctta agcagttcgg cagccttctt
    13381 cttggctttt tcattgggac tcatccaaat gggaccgagt cccaggatgc tgacctgaaa
    13441 acaataccag tagcccaggc catagaaagc cagcagttcg ctatattcgc tgttgcgggc
    13501 ggtatcccag cggcaatccg gatcgcctcc gccgtactgg ctgggtgtga gcaatttggc
    13561 aaaaatctgc tccctcccga taaaatcgac aaactcccga taccagactt tcttgttgta
    13621 atcatcgagc agacgctctt tgcccatttt ctcgaagaaa gctacggtct tatccatgat
    13681 ggctctggaa ccgtcatcgg tcataaggct ctgatatttt ttcggctgca ctaaataatt
    13741 catctgatcc tcctttttac tggaaacaat tataatattt gattatcttg tctttaagac
    13801 agcgtcccga aatccctttg cgggtggtta tcccccgtgt atggggaaac cattctgaaa
    13861 atagaccggt gatagaaaca tgaatattgc attgtggaca tggcggtccg catcctgttt
    13921 ttctaaccga cggctatctc ctgcctgttt aacacaggca gaagagcttt ccaaggcaaa
    13981 attgctctcc atcctcaggc tttaaaccgg atggttttct ttttcggttc gttcttcaac
    14041 aataaaatag aacagggcat cttcaggata atttcatcat tactgctgcc gaccatgaat
    14101 ttctccagcc ggcttt
//
LOCUS       DAONQZ010000059        14016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4852_length_14016_cov_15.292959, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000059 DAONQZ010000000
VERSION     DAONQZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_14016_cov_15.292959"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLR13_08985"
     CDS             complement(<1..1092)
                     /locus_tag="PLR13_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQC19514.1"
                     /translation="MQDVDLIITGSKALLLDEKNTCLNNAAVAVNGDKIIAVDDSKII
                     LKQFEAKKTIEAGDSLIMPGFVNCHTHAAMTCFRGIADDLELMDWLNNYIFPAEAKNV
                     NKELAYWGSLLGAAEMIKSGTTTFCDMYIFEDETARAAKEAGIRCLVGEVLFDFPSPN
                     FKTPEEGVAYTKMLIEKWQDDPLVNIMIEPHALYTCSQPLLADVKKLSEEFNVPIGLH
                     LLENAAEKKQLEEKFGKGAVSFLKDIGYLNEQLVAFHCVHFTEEDMKMFADYGCKVSH
                     NPASNMKLASGVAPVPEMIKAGVTVGLGTDGCASNNNLDMIKEMGTTAKLHKVARLDP
                     TVMDAQTVVRMATIEGAKTLGMEKKIGSLE"
     gene            1273..2067
                     /locus_tag="PLR13_08990"
     CDS             1273..2067
                     /locus_tag="PLR13_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19515.1"
                     /translation="MKKIIMVSFMLLVWASISAASTIGNAVEVSVITDNGRMLPFYQV
                     KSRHNLKKVYAEAVRGDYYRIVVRNKLNRRVGVVIAVDGRNIISGEKSWLKNTERMYI
                     IDPYSTNEYSGWRTGQDKINRFYFTDVPDSYAAAFGDESAMGVIAVAAYPEIRHCPQP
                     PYFSHRTKDGAKNESARDMAAPSALGKSQASESAGTGYGHEEYSPSYRVEFEPERKAV
                     ESILIKYEWRKTLCKMNIIHCGRPYEYSHNRIWENNGYAPPPPCCR"
     gene            2261..2494
                     /locus_tag="PLR13_08995"
     CDS             2261..2494
                     /locus_tag="PLR13_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19516.1"
                     /translation="MAKNKVNAKPAKRKISERDRQLAKVCVNCPVCKHARKTQKGAAF
                     WFVQKIESDLCPFCKAYERVYGRKSHQPVFNKK"
     gene            2647..2958
                     /locus_tag="PLR13_09000"
     CDS             2647..2958
                     /locus_tag="PLR13_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19517.1"
                     /translation="MGISKWLDQKEREGFDVSHVSVPEGMLQEESPAQTIYFKEIRPC
                     SILCTGDHPFATVERFGNWFYCRGRDKEKGPHTTKPQWWMWTRDVELALKTAREHLKD
                     E"
     gene            3018..>4776
                     /locus_tag="PLR13_09005"
     CDS             3018..>4776
                     /locus_tag="PLR13_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19518.1"
                     /translation="MDKKATSLTDIWEAAEHGDVEAQFNLGLMYRSGEGVEQNENKAM
                     SWLLKAADQGFGAAQFYLGVMYHNGDGVAQDYVEAVKWYRKAAEQGHSVAQNTLGWMY
                     ENGKGVSQSYEEAVKWYSREAQQGDACSQYTLGLRYRNGEGINQDDAEAAKWFGMAAE
                     QGLAEAQFNLGLMYRTGDGVAQDHTDALKWFRKAAEQDNAEAQFNLGLIYDEGGSVPR
                     DNAEAVKWYRKAAQHGNAGAQSKLAVKYENGEGVPQDYAEAVKWYRKAAQQGDTEAQC
                     NLGVMYHNGNGVPQNYVEAVKWYRKAAKQGDARAQCKLGVMYHKGNGVKEDHAEAAQW
                     YRKAAEQGNTEAMNNLKAMADKSIAFAQLNLGMMYEEGAGLSRDYAEAVKWYSKAAGQ
                     DLAQAQFNLGLMYLKGDGFPVEGAEAAKWFRKAAVQGFVEAYLHLGLMYRYGEEIAPD
                     DAEAAKWLIKAAEQGSAVAQFNLGVMHTLGEGVPLDDAEAINWFRKAAEQGDPDAGYN
                     LGLLYRTGKGVAVDNAEAAKWFLKAAEQGNVRAQINLADMHQKGEGLPHDEAEALKWY
                     REAAGQGNAYAQSNLGLMYR"
     assembly_gap    4777..4876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4877..7408
                     /locus_tag="PLR13_09010"
     CDS             <4877..7408
                     /locus_tag="PLR13_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19519.1"
                     /translation="AVFRWLHDESREDDAEAQFNLGVMYEEGKGVAQDEAQAATCYRK
                     AAGRGHAGAMNNLGVLFSQGKGVIQNDAEAVMWFLKAIHLDHETALNNLKAMAESGSP
                     IAQLHLGQMYQEGGGVEQDKDEAVRCFLQAAQGGNAEAQFKMGMKYIKGEDVPQDGSE
                     AARWLRKAAGQGDVRAQVYLAFMYQRGEGVTRDDSEAVQWYRKAAEQGNAEAQLNLGE
                     MYQSGKGVPKDDTEAVQWFIKAAGQGDVAAQNNIAAMYLSGRGVEQSDEEAVRWYLQA
                     AELGSAGARNRLGTLYQTGRGVAQDDAEACRWFLQAAEQGNAEAQVHLALIYLNRKDS
                     PADQAETCKWFQQAAAQGNMVAQYHLGLMYEQGKGVAQDDAEAVKWYREASRQGHAAA
                     LNYLRTMSDRGHAAAQFNLAEMYQNGEGMIQDAAEAVKYFQQAAGQGHVEAQFALGVK
                     YENGQGVEQDDAEAVKWYRMAAEQGHADAQCNLGVKYTTGQGVSKDDAQAVQWFLKAA
                     EQNNITALNNLGVIYQMGEGVARDDAEAAKWYRKAAERGHVQAQINLGLMYRNGEGVE
                     QDDEEAVQWWKKAADQGDPVAQNNLGVMYLNERGVSRDDAETAKWFRKAADQGHADAQ
                     NNLGVMYLNGKGVPRDYSEAITWYRKAAEQGNPKAQYNLGLMYRNGEGVPRDDAEAIE
                     WFLKAARQDEAGAQCILGLMYEEGKGTRQDAAEAVMWYRRAARQGDEEALNNLTAMCN
                     RGNSIAQFNLGLMYEESEGVLQDEAEAAKWYRRAAEQEHEDALNRLMEMCERGIAVAQ
                     FNLGGMYQDGEGVPQDDEEAAKWYRKAAEQGHATAKLRLDKMKDK"
     gene            7506..9746
                     /locus_tag="PLR13_09015"
     CDS             7506..9746
                     /locus_tag="PLR13_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19520.1"
                     /translation="MNASSLTDIRKAAEQGNIDASNNLGVMYLTGKGVPQDYTEAAKW
                     FRMAAEQGDGRAQFNLGWMYQEGKGVPRNDAEAAKWYLQAAAQGNARAQFNLGIMYQE
                     GEGLAQNDAEAAKWYLKAAEQGIATAQFNIGVMYQEGLGVDQDYEQALKWLRQAAKQG
                     EVDAQTIMGIMYKEGHGVVQDYAKAAKLFLKAAELDDAKAQFHIGWMYQEGKGVPQSD
                     EEAARWYIKAAEGGDPNAQFNLGLMYHNGEGIDQDDEEAVKWYRAAAEQGHMEAQNWL
                     AAIYREGIFVAQDDAEAVKWYIKAAERGDAEAQNLLGMMYVEGKGVKQDYAEAARWYR
                     KAADQGHAEAKSALEIISSLEEEVETQEDTRETPEERKEESIKDERAAEKPFADLVEA
                     EREPVAHETAEANRESVSIKESENNIETPLEAKEEIESAGGISALPEAAAETKVEEPA
                     PEEDVLPENEEAAVQWYLRKAEKGDVDAQFHLAFIYREGRGVTQDYAESVRWYLKAAE
                     QGDISAQSALGNMYLTGEGVEQNDAAAVRWLLKAAQQGDAVAQNHLGMLYLTGRGVVQ
                     DPAEAVMWYVQAAEQGQVNAQLVLGKMYKDGTGVPQDYTEALKWFLKAAENDDPVAQF
                     NLGWMYQEGEGTQHNLAEAVNWYHKSAQQNNAEAQFNLGLKYFYGEGIAQDFEEAVKW
                     YRAAAEQDHIEAQIWLGDIYQDGIFVPQDNAEAEKWYARAARQGDEKAQSKLNAMKKQ
                     SAKEKQ"
     gene            10015..10185
                     /locus_tag="PLR13_09020"
     CDS             10015..10185
                     /locus_tag="PLR13_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19521.1"
                     /translation="MLKVLGVIINLGLVIYLGLQLSQYMGPEKIAGLNTFQNPMIIAF
                     VAVVALFIALKN"
     gene            10285..11349
                     /locus_tag="PLR13_09025"
     CDS             10285..11349
                     /locus_tag="PLR13_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQC19522.1"
                     /translation="MLKPKAIITVLAVCLLFMTVAFAQTEDVKEHKTVAGDTLWGIAH
                     KELKDPFMWPAIWKANPDIKNPRWIYPGQIIRIPSSLIQKDESDKDAVRKPKETYREP
                     AQEEAVDIKKEAAVVSFPVVNEHLIVSAGYIAEMIPVIGKVDECMTGKAAFRNTNPDV
                     GQVNDSASGRTLYGVNDVVYLNVDQPAKAGDKFYVVHISDEVIHPITKDTIGHVVTIS
                     GIAKVLEVKNGDTRARIIKSYSEIARGDILDTYYEIKPVMTTGKFRSPDVNGMIIAAS
                     RQTIVSASEDIVYLDRGCKDGIEIGDMFKTMAVNHHTVTNGTIQVINCKDHTATAIIR
                     KSDAPIVPGNIFAKLEEQVK"
     gene            11535..11936
                     /locus_tag="PLR13_09030"
     CDS             11535..11936
                     /locus_tag="PLR13_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQC19523.1"
                     /translation="MKMPKKILWLVLLLFMIAGCQTISQSLIGDNTALYYMPGGAEGF
                     ARYYANSFNGKRTTSGEIYQSKKLTAAHPTLPLGTLVKVVNLENNKSVVVKINDRCLE
                     HEDVFIDLSREAARRLGFIKQGKARVRITVI"
     gene            complement(12030..13256)
                     /locus_tag="PLR13_09035"
     CDS             complement(12030..13256)
                     /locus_tag="PLR13_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HQC19524.1"
                     /translation="MKISLIELYHVQVPLKQTFWPTWIPGYPQTHNGFTLIKLVTDDG
                     IEGYSAGSALGYEREGLGDLIGGYLLGADPTDIESCQNLLKQAGILGWRNFWIEPACW
                     DIIGKSKGKPVYELLGGEARPVEVYCSTGEMHEPEKRVEELLAMKQRGFKTFKLRVKN
                     KALKDDIRHIEVVRKGVGDDLILGVDANQGWLVTIVDKIPAWDLKRATEFADACYANN
                     YNWLEEPLDSRNYDGNAALKKISKVKISGAELNYGWDEIKIMFEKDCFHIYQPDATFA
                     GGIAQVKKVIDACHTHNREYSPHTWTNGIGFYINWNMVLADKGNKLPLEYPLEEPSWV
                     PEQREGIIDPIIPDKNAMLAPFTRPGLGFEINQKLLNKYGKCFFKFTPTRQTIKVIRQ
                     KGLKAALELKKRKETL"
     gene            13440..>14016
                     /locus_tag="PLR13_09040"
     CDS             13440..>14016
                     /locus_tag="PLR13_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19525.1"
                     /translation="MNLNNPEATQQALSLLRSGDPFQWYVIPLLVIVLYIYANEYEKG
                     NYRTIAGGLALYMVHWFVEIVNALIQYFSGHALWTVPAGSAFVIMVGVGIEISLMFSI
                     AGLVVCKLMPKDPTVKILGINNRLVFSIANAAFASILEIFLVRTPTFVWVYNWWGAFP
                     VFVTVYIPFFAAACYVHDWEPRAQKLFIGSLF"
BASE COUNT         3644 a         3135 c         3935 g         3202 t
ORIGIN      
        1 ttccagtgaa ccgattttct tttccattcc cagagttttt gcgccttcaa tggtggccat
       61 acgtacaacc gtttgcgcgt ccatgactgt aggatcaagc cgggcgactt tgtgcagttt
      121 agctgtcgtg cccatttctt tgatcatgtc caggttattg ttgctggcgc agccatcggt
      181 accgaggccg acggtaacgc ctgctttaat catttccggc acgggagcca cgccgctggc
      241 gagtttcatg ttgctggcgg gattatggga aactttgcag ccgtaatcgg caaacatttt
      301 catgtcttct tccgtaaaat gaacgcaatg aaacgcgacg agttgttcgt tgagatatcc
      361 gatgtctttc aggaatgaaa cagcgccctt accgaatttt tcttccaact gttttttttc
      421 ggcggcattt tcgagcagat gcagtcctat gggtacatta aattcttcgg acaatttttt
      481 gacatcggcc agcagcggtt gagaacaggt gtaaagggca tgcggctcaa tcatgatatt
      541 gacgagcgga tcatcctgcc atttttcaat cagcattttt gtgtaggcaa cgccttcttc
      601 gggcgtcttg aaattaggag aaggaaaatc gaaaagcact tcaccgacga gacagcggat
      661 gcctgcttct ttggctgcgc gcgccgtttc atcctcgaag atgtacatat cgcaaaacgt
      721 ggtcgtgccg gatttaatca tttcagcggc gccgagcaag gaaccccagt aagccagttc
      781 cttattcacg tttttggctt cggcggggaa gatataatta ttgagccagt ccatcagttc
      841 caggtcgtcg gcaatgccgc gaaaacaggt catggctgcg tgtgtatggc aattgacgaa
      901 accgggcatg atcagagaat ccccggcttc gatggttttt tttgcctcga attgttttaa
      961 tattattttc gagtcatcga cggcaataat tttatcaccg ttgaccgcca cagccgcatt
     1021 gttcagacag gtattttttt catcgagcaa cagcgctttc gatccggtga taattaaatc
     1081 gacatcctgc atagggagtt tcctttttag ttttttgatt tcttaacaca acttttttga
     1141 attgtcactt tgtccgtgct acgcctgcga ggcaatcttt acggggccaa aaaaaaggga
     1201 actttatggg ctcttctttt gtctaacctg caaaaggcaa gcaaaagccg gaaaatttag
     1261 aaggaggaac atatgaaaaa aataataatg gtcagcttta tgttgttggt ttgggcaagt
     1321 atttccgcgg cgtcaactat tggtaatgcg gttgaggtca gtgttattac cgataatggc
     1381 aggatgctgc ctttttatca ggtcaaaagc cgccataatc tgaagaaggt ttatgctgaa
     1441 gccgtcaggg gtgattatta ccggattgtc gtacgcaaca aactgaatcg ccgggtgggc
     1501 gtggttattg cggttgacgg caggaatatt atttccggtg aaaaatcatg gctgaagaac
     1561 acggaacgga tgtatattat tgacccgtat tccaccaacg aatattcagg atggcgcacc
     1621 gggcaggata agattaaccg tttttatttt accgatgttc ccgattccta tgcggcggcc
     1681 ttcggcgacg aatccgccat gggtgtgatt gccgttgccg cttacccgga aatccggcat
     1741 tgtccgcaac ctccgtattt ttctcaccgg accaaagatg gagcaaaaaa cgagtcagcc
     1801 cgagatatgg cggcaccttc cgctctgggc aaatctcagg cctctgaaag cgcgggcacc
     1861 ggctacggtc atgaggaata ttcgccttcc tatcgcgttg aatttgaacc ggaaagaaaa
     1921 gctgttgaat ccatcctgat caaatacgaa tggcgcaaga cactctgtaa aatgaatatc
     1981 atccattgcg gcaggccgta tgaatattct cacaaccgca tctgggaaaa taacggttat
     2041 gctcctcccc cgccatgttg ccgctgaata acataagatt gtcgacgaaa ggcagaagac
     2101 gtttcttctg cctttctttt tgaacacgtt gttttgcggt taaaaaatgg tccatgatcc
     2161 atgatatttt attgctaaaa gaactttgac acaaaaattt catattggta tacttacaag
     2221 gctttagtta aatttattca atcatttgtg agagactgcc atggcaaaaa acaaagtgaa
     2281 cgcaaagcct gcaaagcgca aaatttcgga aagagaccgc cagctggcca aagtctgtgt
     2341 gaactgcccg gtatgcaagc atgcccggaa aacacaaaaa ggcgccgcat tctggtttgt
     2401 tcaaaaaatc gagagcgatc tctgtccttt ttgtaaagct tacgaacgtg tgtacggccg
     2461 gaagtcgcat cagccggttt ttaataagaa ataattatac tgttttcggg attcccggca
     2521 agcaggcagg gggataaaat agaaataccc atccactcgg ggtatctctt tttattcaat
     2581 gcctatttac ccattcccga aaggcaaaaa acacggtcgc catatcaatt acaggagaag
     2641 atcgtcatgg gaatttcaaa atggctggat cagaaagaaa gagaaggctt tgatgtttcg
     2701 catgtttcgg tgcctgaagg aatgcttcag gaagaatccc cggcccaaac gatttatttc
     2761 aaggagattc gcccctgttc cattctctgc accggggatc atccttttgc caccgtggaa
     2821 cgcttcggta actggtttta ttgccgcggg cgcgataagg aaaaggggcc gcacacgacg
     2881 aaaccacagt ggtggatgtg gaccagagat gttgaactgg cgctgaaaac cgccagggaa
     2941 catctcaaag acgaataacg gatgaaatcg tttgcggatg tccgatgtca ggaaaaatat
     3001 tttagagacg agtgccgatg gataaaaaag caacatcctt aaccgatatc tgggaagcgg
     3061 ctgaacacgg agacgtggag gctcagttca acctcggttt gatgtaccgg tccggcgaag
     3121 gcgttgaaca aaatgaaaac aaggccatgt cctggctttt gaaagcagcc gatcagggat
     3181 ttggagccgc tcagttttat ctcggggtga tgtatcataa cggcgacggc gttgcccagg
     3241 attacgtcga agcggtcaaa tggtatcgca aagccgctga acagggacac tccgtggcgc
     3301 aaaataccct gggatggatg tatgaaaatg ggaaaggcgt ttcgcaaagc tacgaggaag
     3361 cggtgaagtg gtatagccgc gaagcccagc agggggatgc gtgttctcag tacactctgg
     3421 gattaagata ccgcaacggc gagggcatca atcaggatga tgcggaagca gccaaatggt
     3481 tcggcatggc ggccgaacag ggacttgcgg aagcgcagtt caatctgggc ctgatgtatc
     3541 gcaccggtga cggcgttgcg caggatcata ccgatgcctt gaagtggttc cgcaaggccg
     3601 ccgaacagga caatgcggaa gcccagttca atctcgggct gatatatgat gaaggtggaa
     3661 gcgtgccgcg ggacaatgcc gaggcggtga aatggtaccg caaagccgcc cagcacggaa
     3721 acgcgggagc gcagagtaaa ctggctgtta aatatgaaaa tggagaagga gtccctcagg
     3781 attacgccga agcggtcaaa tggtatcgta aagccgctca acagggtgac acggaagcgc
     3841 agtgcaatct gggcgtaatg tatcacaatg gtaatggcgt tccgcaaaac tatgtggaag
     3901 ccgtcaagtg gtatcgtaaa gcggccaagc agggcgacgc cagagcgcag tgcaaattgg
     3961 gcgtgatgta ccataaaggc aacggtgtca aggaggatca tgccgaagcg gcgcaatggt
     4021 atcgtaaagc cgccgagcag ggcaataccg aggcgatgaa caatctcaag gccatggctg
     4081 acaaaagcat cgcttttgcg cagttgaacc tggggatgat gtatgaggag ggcgcaggtt
     4141 tatcccgtga ttacgcggaa gccgtgaaat ggtacagcaa ggccgccgga caggatcttg
     4201 cccaggcgca atttaatctg gggttgatgt atcttaaagg cgacggcttt ccggtggaag
     4261 gcgccgaggc ggcgaaatgg tttcgtaaag cagcggtaca gggattcgtg gaagcgtatc
     4321 tccacttggg attgatgtat cgatatgggg aagaaattgc cccggacgat gccgaagcgg
     4381 ccaaatggtt aattaaggcg gctgaacagg gcagtgctgt cgcgcagttt aacctgggcg
     4441 tgatgcacac actgggcgaa ggggtccccc tcgatgatgc cgaagcgatc aactggtttc
     4501 gtaaagccgc cgagcaggga gatccggatg ccgggtacaa tctcggtttg ctttaccgga
     4561 ccggcaaggg cgttgctgtg gacaacgcgg aagcggccaa gtggttttta aaagccgccg
     4621 agcaggggaa tgttcgcgct cagatcaatc tggccgatat gcatcagaaa ggggaagggc
     4681 tgcctcatga cgaagccgag gcattaaaat ggtatcgtga agcggccggg cagggtaacg
     4741 cgtatgccca gagcaatttg ggcttgatgt accggannnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnngctg tgtttcgctg gctgcatgac gagtcacggg aagacgacgc
     4921 cgaggcccag tttaatctgg gtgtcatgta cgaagagggt aaaggcgttg ctcaggatga
     4981 ggctcaggcg gcaacatgct atcggaaggc cgccgggcgg ggacatgccg gagcgatgaa
     5041 taatctgggc gttctattct cgcaggggaa aggggttatt caaaatgacg ccgaagcggt
     5101 catgtggttt ctcaaggcga ttcatctaga ccatgaaaca gctctcaata atctgaaagc
     5161 catggctgaa agcgggagtc ccattgcgca attacatctg ggacagatgt atcaggaagg
     5221 cggcggtgtc gaacaggaca aggacgaagc ggtacggtgc tttttacagg ccgcgcaggg
     5281 aggcaatgcg gaagcccagt ttaaaatggg catgaaatat atcaaagggg aagatgtgcc
     5341 tcaggatggc agcgaagctg cccgatggct tcggaaggcc gccgggcagg gagatgttcg
     5401 cgcccaggta tatctggcct ttatgtatca gcggggcgaa ggcgtcactc gtgacgatag
     5461 cgaagcggtg cagtggtatc gcaaggcggc tgaacagggc aatgccgaag cgcagctcaa
     5521 tctgggcgaa atgtaccaaa gcggcaaggg cgttccgaag gacgacaccg aggccgttca
     5581 atggtttatc aaagccgccg gtcagggaga tgtggcagcg cagaataata ttgccgccat
     5641 gtatctgagc ggcagaggcg tcgagcagag cgatgaagaa gccgtccggt ggtatctgca
     5701 ggctgccgaa cttggaagcg ccggggcgcg caaccggttg ggcactctgt atcaaacagg
     5761 gagaggtgtt gcgcaggatg atgccgaggc ctgtcgatgg tttctgcagg cggccgagca
     5821 gggcaacgct gaagcgcagg ttcatcttgc tttgatttat ttaaacagaa aagattctcc
     5881 ggcggatcaa gccgagacct gcaaatggtt ccagcaggcg gctgcacagg ggaatatggt
     5941 ggcccaatac catcttggtc tgatgtatga gcagggaaaa ggcgttgcgc aggatgacgc
     6001 tgaagccgtc aagtggtatc gtgaggcctc ccggcaggga cacgctgctg cgttgaatta
     6061 tctccggacg atgagtgatc gcggccatgc cgctgcgcaa ttcaatctcg ctgagatgta
     6121 tcagaatggc gaaggtatga tccaggatgc tgccgaagcc gtaaaatatt tccagcaggc
     6181 cgcaggacag gggcatgttg aggcgcagtt tgctcttggc gtaaaatatg aaaacggtca
     6241 gggtgttgag caggacgatg ccgaggcggt gaagtggtat cgcatggcag cggagcaggg
     6301 acatgccgac gctcaatgta atttgggtgt aaaatacaca accggtcagg gggtttcgaa
     6361 ggacgatgct caggcggttc aatggtttct caaagccgcc gagcagaata atattactgc
     6421 cttgaacaat ctgggtgtga tttatcaaat gggagaagga gttgcacgag acgatgccga
     6481 ggctgccaaa tggtatcgta aagcggcaga gcgcgggcat gtgcaggccc agattaatct
     6541 cggtttgatg tatcgcaacg gcgagggtgt cgagcaggat gacgaagaag cggtgcagtg
     6601 gtggaagaag gcggctgatc agggcgatcc cgtcgcgcag aataatctgg gtgtaatgta
     6661 tctgaatgag cggggtgttt cacgggatga tgccgagacg gctaaatggt ttcgtaaagc
     6721 ggccgatcag ggacacgccg atgcgcagaa taatctgggc gtgatgtatc tgaacggcaa
     6781 aggcgttccc cgggattatt ccgaagcgat aacatggtat cgcaaggcgg ctgagcaggg
     6841 aaatccaaaa gcccagtata acctggggct gatgtatcgc aacggtgaag gcgttccccg
     6901 ggatgatgcc gaggcgatcg aatggtttct caaagccgcc cggcaggatg aggcgggcgc
     6961 ccagtgtatt ctcggtctga tgtatgagga aggcaaggga accaggcagg atgccgcgga
     7021 agcggtgatg tggtatcgaa gagccgcccg tcagggagac gaggaagcgt tgaataatct
     7081 gacggccatg tgtaaccggg gcaactccat tgcccagttc aatctcggct tgatgtatga
     7141 ggagagcgaa ggtgttctgc aggacgaagc cgaagccgcg aagtggtatc gccgggcggc
     7201 ggaacaggaa cacgaagacg cgttaaacag actgatggaa atgtgtgaaa ggggcattgc
     7261 cgttgcgcag ttcaatctgg gcggtatgta tcaggacggc gaaggagttc ctcaggacga
     7321 tgaggaagcc gcgaagtggt atcgcaaagc cgccgaacag gggcatgcca cagccaaact
     7381 cagactggat aaaatgaagg acaaataaga tacggcagaa aggctctatc ttataaaaaa
     7441 ggcaacggag gcgtcaggaa atattttgaa tcgatcgtat tttcctgata agaattgatg
     7501 gaaaaatgaa tgcatcatcc ttaaccgata ttcgtaaagc cgctgagcag ggaaacatcg
     7561 acgccagcaa caatctgggt gtcatgtatc ttacggggaa aggtgtgcct caggattata
     7621 ccgaggcggc caaatggttc cggatggcgg ctgaacaggg agatggccgg gcacagttta
     7681 atctcggctg gatgtatcag gaagggaaag gtgtgccgcg aaacgacgcg gaggcggcca
     7741 aatggtatct gcaggcggct gcccagggca acgcccgcgc ccagttcaat ctcggtatca
     7801 tgtatcagga gggtgaaggc ctggcgcaaa acgatgccga ggcggccaag tggtatctga
     7861 aagccgccga gcagggtatt gcgaccgccc aattcaatat cggcgtgatg tatcaggagg
     7921 gactgggtgt cgatcaggat tatgagcagg cgctcaagtg gctccggcag gcggccaagc
     7981 agggagaagt ggacgcccag acgatcatgg gcatcatgta taaggaaggt cacggcgttg
     8041 ttcaggatta cgcaaaagcc gcaaaattgt ttctcaaggc agcggaactc gacgatgcca
     8101 aggcgcagtt tcacatcggc tggatgtatc aggaaggcaa gggtgtcccc cagagtgatg
     8161 aagaagccgc ccgctggtat atcaaagccg ctgaaggagg agatccgaat gctcagttta
     8221 atctcggcct gatgtatcat aacggagaag gtatcgatca ggacgatgaa gaagccgtta
     8281 aatggtatcg cgctgcggcc gagcagggac acatggaggc tcagaactgg cttgccgcca
     8341 tatacagaga aggcatattt gtcgcacagg atgatgccga agccgtcaag tggtacatca
     8401 aggcggccga acgcggggat gccgaggctc agaacttgct gggcatgatg tatgtggaag
     8461 gcaagggggt aaaacaggat tatgccgaag cggcccggtg gtatcgtaaa gcggccgacc
     8521 aggggcacgc ggaagcaaaa agtgcgctgg agattatttc ttccctggaa gaagaggtgg
     8581 agacgcagga ggataccagg gaaacaccgg aagaacgcaa agaggaatca ataaaggacg
     8641 aaagagccgc ggaaaaaccg tttgccgatc tggtcgaagc ggaaagagag ccagtcgctc
     8701 atgagacggc tgaggcgaac cgggaatcgg tgagcatcaa agaaagtgaa aacaacatcg
     8761 agacaccgtt ggaggcgaaa gaggagattg aatcagccgg aggaatatca gcgttgccgg
     8821 aagctgcagc cgagacgaaa gttgaagaac ctgctccgga ggaggatgtc ctgccggaga
     8881 atgaagaggc ggcagtccaa tggtacctca gaaaagctga aaaaggcgat gtcgatgccc
     8941 aatttcatct tgcgtttatt tatcgcgagg ggcggggcgt tacacaggat tacgccgaat
     9001 ccgtcagatg gtatctgaag gcggccgaac agggagacat ttctgcccag agcgctctgg
     9061 ggaacatgta tttaacgggc gagggtgtgg agcaaaatga tgccgcagcg gtccggtggt
     9121 tactaaaagc cgcccaacag ggagatgcgg ttgcgcagaa tcatctgggc atgctctatc
     9181 tcacgggcag gggtgttgtt caggatcccg ccgaggccgt gatgtggtat gtccaggcgg
     9241 ctgaacaggg acaggtcaac gcccagctcg tcttgggcaa aatgtacaag gacggcacgg
     9301 gtgttccgca ggattatact gaggcgctga aatggtttct caaagccgcc gaaaatgacg
     9361 atcctgtcgc gcaatttaat ctcggatgga tgtatcagga aggtgaagga actcagcaca
     9421 atcttgcgga agccgtaaac tggtatcaca agtcggcaca gcagaacaat gccgaagccc
     9481 agttcaatct gggattaaaa tatttctacg gcgaaggaat cgcacaggat tttgaagaag
     9541 ccgtcaaatg gtatcgcgcc gccgccgaac aggaccatat cgaagctcag atctggcttg
     9601 gcgacatata tcaggacggg atttttgtgc cccaggataa tgctgaggct gagaaatggt
     9661 atgcgcgggc ggcccggcag ggggatgaaa aagcacaatc gaaactcaat gccatgaaaa
     9721 aacaatccgc aaaagaaaag cagtaaaacg ggtttttcca aaaataagcc gcatcgcaca
     9781 gtgaatctgc caggcagatt taaataatcc tgtaaacaat aattcattag cccgccggtg
     9841 gctgtgcatt ttcatttgcg tggcgccaat cagaaaggta tcgtgccgtc aagattgtct
     9901 tgacatcatt gctgacgttt tctatattta cacagcttat tatcaccgta atcatttaat
     9961 tgtagcgaca gaccaaacct gacgttaacg gatttctgga aaaaggagat tcctatgctg
    10021 aaagtgctgg gtgtgataat caatttggga ctggtgatat accttgggct tcagttgtca
    10081 caatatatgg ggccggaaaa aatcgcaggt cttaacacct ttcaaaaccc catgatcatt
    10141 gcgtttgttg ctgttgttgc attatttatc gctttgaaaa attagcgtta taattttaaa
    10201 atagaatgct gaatattttt atggaattta aatcaatttt ggattcttcg attaaaagga
    10261 gtaattcaaa gaaggagacc catcatgttg aaacctaaag ccataatcac tgtccttgct
    10321 gtttgcctgc ttttcatgac cgtcgctttt gcgcagacgg aagatgtgaa agaacataaa
    10381 acagtggcag gcgatacgct gtggggtatt gcgcataaag aattaaagga tcccttcatg
    10441 tggcccgcga tctggaaagc caatcccgat ataaaaaatc cccgatggat ttatccgggc
    10501 cagatcatca ggattccttc ttctctgatt caaaaagatg aaagcgataa agacgcggtg
    10561 cgcaaaccga aggaaacata tcgggagccc gctcaggaag aagccgtgga tatcaaaaag
    10621 gaagccgctg tggtttcctt tcccgtagtc aatgaacatc tgattgtgtc tgccggctat
    10681 attgcagaaa tgattccggt gatcgggaag gttgacgagt gcatgaccgg gaaagccgct
    10741 ttccggaata cgaaccccga tgtcggacag gtgaacgatt ccgcgtccgg caggacgctg
    10801 tacggtgtca atgacgtggt ctatttaaac gtcgaccagc ccgccaaggc gggagataaa
    10861 ttttatgtgg tccatatttc cgatgaggta atacacccca ttacgaaaga tacgatcggc
    10921 catgttgtta cgatcagcgg catcgcaaag gtgctggaag taaaaaatgg cgataccagg
    10981 gccagaatca ttaaatccta cagcgagatt gcccgggggg atattcttga tacgtattat
    11041 gagattaaac cggtcatgac cacgggaaaa ttccgcagtc ctgatgtcaa cggcatgatt
    11101 atcgccgcgt cccggcagac gattgtcagt gcgtccgaag atattgttta tctcgacaga
    11161 ggatgcaaag acggtattga aataggggat atgttcaaga ccatggcagt gaatcatcat
    11221 accgtcacga acgggaccat tcaggtcatc aactgcaagg atcatacggc tacggcgatt
    11281 atccggaaaa gcgatgctcc gattgttccc ggaaatattt ttgcgaaact ggaagaacag
    11341 gttaaatagg cgttgcaagg aaaaacgaaa cacgcgatcc tggatgcggt gacggttatg
    11401 tcggagtgta tgaaatttgc tccgggatga cgacggggat gcgaaaatat aagaatgtta
    11461 aaaatcgggc ctgtgaaaaa agccgcattg ttttgccgga cgttgaagat ccatcaggtc
    11521 tcggggagaa cgccatgaag atgccaaaaa aaatcctatg gctggtttta ctgttgttta
    11581 tgatcgccgg ctgtcagaca atttcacaat cattgatcgg cgataatacg gctctgtatt
    11641 atatgccggg tggcgcagaa ggatttgccc ggtattacgc gaacagtttc aacggtaaaa
    11701 ggacaacgtc cggtgaaatc tatcagtcaa aaaagctgac ggcagcccac ccgaccctgc
    11761 cgctgggaac cctggtcaaa gtggtgaatc tggagaacaa taaatcggtg gtcgtcaaaa
    11821 tcaatgaccg atgtcttgaa cacgaggatg tttttattga tttgtcgcgt gaagcggccc
    11881 gccggctggg ttttataaag cagggcaagg ccagagtccg gattacagtt atctaaaaga
    11941 caaaatttca aacatgaacc cggctgcttt tgtaaagccg ttccttactg tattttcgct
    12001 gtcaaaaacc gcgacaggat aataatactt tacagtgttt cttttctctt cttcagttcc
    12061 agcgctgcct tgagtccttt ttgccgaatg acttttatcg tctgtcttgt cggagtgaat
    12121 ttgaagaagc actttccgta tttgttcagc agtttctgat tgatctcaaa accgaggccc
    12181 ggtcgcgtaa aaggcgccag catcgcgttt ttatccggaa taatcgggtc gataatgcct
    12241 tcgcgctgtt cgggaaccca ggacggttcc tccagcgggt attcgagcgg cagtttgttc
    12301 cccttgtcgg ccagcaccat gttccagtta atgtaaaatc cgatgccgtt cgtccaggtg
    12361 tgcggcgaat attccctgtt gtgggtgtgg caggcatcaa tcactttttt cacctgcgcg
    12421 atccctcccg cgaaggttgc gtcgggctga taaatgtgaa agcaatcttt ctcgaacatg
    12481 attttgattt catcccagcc gtagttgagt tcagcgccgg aaattttcac tttggagatc
    12541 ttcttcaaag cggcattgcc gtcataattt ctcgaatcca gaggctcttc cagccagtta
    12601 taattgttgg cgtaacaggc atcggcaaat tccgtggcgc gtttcaaatc ccacgcggga
    12661 attttatcca cgatggtcac cagccatccc tggttggcgt ccacgcctaa aatgagatcg
    12721 tcgccgacac cttttctgac cacctcgatg tgccggatat catccttcag cgccttgttt
    12781 tttacacgca atttgaacgt tttgaatccg cgctgtttca tggccagaag ctcctcgacc
    12841 cttttttccg gttcatgcat ctcgcctgtg gaacaataca cttcaacggg cctggcctcg
    12901 ccgcccagaa gttcataaac cggcttgccc ttgctcttgc cgataatgtc ccagcatgcc
    12961 ggttcgatcc agaaatttct ccagcccaga atgccggcct gttttaataa gttctgacac
    13021 gattcgatgt ccgtcggatc ggcgcccaaa agatagccgc cgatgaggtc gcccaacccc
    13081 tctctttcat aaccgagggc ggagcccgcc gaataccctt cgataccgtc atcggtcacc
    13141 agtttgatca gcgtaaagcc gttgtgtgtc tgcgggtaac ccggaatcca ggttggccag
    13201 aaagtctgtt ttaacgggac ctgtacatga taaagttcaa tcaaagatat tttcataaaa
    13261 acctccttga tccgtttcct tacaatctac acccgctgcg gtgctttgtc aaaccggctt
    13321 gatctacggc cgggtttcca aaagtccgga ctgttatttt tttctgtttc catgtagaat
    13381 aaacgacaaa aatcacatgg aatccgacaa aaaattgaat acaaaaagga ggaacagcaa
    13441 tgaatttgaa caatcctgaa gcaacccagc aggcattgag ccttttgaga agcggcgatc
    13501 cttttcagtg gtatgttatt cccctgctcg tgatcgtctt atatatttat gccaatgaat
    13561 atgaaaaagg caattaccgt accattgccg gcggactggc tctttacatg gtgcactggt
    13621 tcgtggagat cgtcaacgcc ctgattcagt atttctccgg ccatgcgctg tggaccgttc
    13681 ccgccggatc ggcctttgtc atcatggtgg gggtcggcat tgaaatcagt ctgatgtttt
    13741 ccattgccgg actggttgtc tgcaagctga tgcccaaaga tccaacggta aaaattctgg
    13801 gcatcaacaa ccgcctggtc ttctccattg ccaacgccgc tttcgcctcg attctggaaa
    13861 tatttctggt gcgcacgccg acctttgtct gggtatacaa ctggtggggt gcctttcccg
    13921 tttttgtgac ggtttacatc ccgttcttcg cagccgcctg ttacgtgcat gactgggagc
    13981 cgcgcgcgca gaaacttttc atcggttcgc tttttg
//
LOCUS       DAONQZ010000060        13923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_4892_length_13923_cov_14.197505, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000060 DAONQZ010000000
VERSION     DAONQZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13923
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4892_length_13923_cov_14.197505"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLR13_09045"
     CDS             <1..245
                     /locus_tag="PLR13_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HQC19526.1"
                     /translation="HQDYENWLENLAPHEPVAHYRHNRTGEDNGDAHLKRQIMGREVV
                     VAVTNGQLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(379..1644)
                     /locus_tag="PLR13_09050"
     CDS             complement(379..1644)
                     /locus_tag="PLR13_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HQC19527.1"
                     /translation="MIFKKKTSHGFTFLISFIIVIFAVMFLATDVHALDISNMYSPLN
                     KKRPLRSKTQYIILHTTEGALNGSLRKVHQFGETHYFVTPTGKVYRIIDRKKIATHAG
                     RSMWNRQTTLDNVSIGIEVVGFHDQDITRAQYTALRQLLRQLKKIYRIKDKNILTHSM
                     VAYGRPNIFHPRKHRGRKKCGMIFSHPDVRKLLGLLPGPKRDPDVAAGRLVVADPDLH
                     RFLYKPPKVLAALKGTKDSSGSRSMVIAQGNNAWRIARDKYDAPSTIYTYPNGKKLRG
                     NEIRKWDSIPVGTRVSFVDEDREQEFEKFLEIGKDGNTVQDLAGNLYADPTTIYFFPD
                     GLIRTGAEMKKSSSLRKLLKNPPKGTRLLVGYIYGGYVQKGRSATRIAGRKWNYPSTF
                     YRMPDGRILNGDNVEKKSIPSGTLIFIMK"
     gene            complement(1712..2500)
                     /locus_tag="PLR13_09055"
     CDS             complement(1712..2500)
                     /locus_tag="PLR13_09055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQC19528.1"
                     /translation="MSFSSDTLDRGGVILVTVKTNEQKKPRITWLQKKVPLVYSTSDE
                     SWSGFISADLHQETGLYDCMIRETSSGFQERYPIRIRLKDYGVRRLKLPKTKVELDAH
                     SLKRVKNEAAVIHSLWPAGDTSPKWKGHFVMPVDGDIVGTFGKKSIINQLHRSPHTGV
                     DLAGKKGDPVVAINHGRVLLAADHFFSGKSVYVNHGGGIISMYFHLDKILVNKGDDIH
                     KGEVIGLLGATGRVTGPHLHWGVRINGNRVDPLTLIALSGTLEE"
     gene            2954..3532
                     /locus_tag="PLR13_09060"
     CDS             2954..3532
                     /locus_tag="PLR13_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQC19529.1"
                     /translation="MMENKKDSIPDLAECEDLMVRYAMLPNIVEHSRQVMRVSLAITD
                     HLQEGLSINRDLIVAAALLHDITKTRSLQTGEKHADSGGVLLRKLGFRRVAEIVEQHV
                     TIPDLNLQGGLEEREIVYYADKRVMHNVIVGLDERVDDLIKRYATDEKIRRRILLNKN
                     QALAVERKISGFMKMDIHQAIGKLVAHDRDKP"
     gene            3566..4252
                     /locus_tag="PLR13_09065"
     CDS             3566..4252
                     /locus_tag="PLR13_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQC19530.1"
                     /translation="MERKKLTITIQQGENVSAVLTLPPDKKKHYRAGVIVAHGAGNDM
                     EHEMLVDFSDGLARAGYPSLRFNFPYKEKGLKAPDGQKKLEATWGAVFLHFQTDMPFQ
                     VDHVIASGKSMGGRVASQMAAEKQLPADGLIFLGYPLHPAGDLTKTRDAHLYRLDVPL
                     LFFAGTKDALCDMILFRKVLKNLSAPWQLDVIEGGDHSFHVPKSLGIRQSAIHDRIVK
                     TSGAWLDGQF"
     gene            4288..4743
                     /locus_tag="PLR13_09070"
     CDS             4288..4743
                     /locus_tag="PLR13_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19531.1"
                     /translation="MENIFDKIKSNLKKGMEEGIAVLREGATVVSVRMNELSEEGKRQ
                     YKIFNLNLKIQEQIKELGCMAYAALDKGKRLDDDKKIKAAYSKIKKIEWQLTKTEGQK
                     KVKTPSAGKTAAKPGAKRAKASPKKAKPQSAKAAGKSSAPGTGPKATSG"
     gene            complement(4771..5682)
                     /locus_tag="PLR13_09075"
     CDS             complement(4771..5682)
                     /locus_tag="PLR13_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQC19532.1"
                     /translation="MTLKGILTVVRIPFLILALILGILGAAVAWYESVRYGSPFNLCY
                     AVLATIGLLIAHAAVNIFNDYFDARSGLDYKTNRTPFSGGSGAVPTGLLTVHESLQLG
                     IICFVVLVPIAVFFIMKSGWMLLPLLIAAMLLIVLYTPFILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGQYAPTALVASMPSYFLVHNLLLLNEFPDAEADVTVKRRTLPIVAGKKN
                     AAFFFSGVTLLVYIWVLGAVIWGSMPVFTLVCFLTMPLALQVIRGSFRYDERELLMDT
                     MKKNVLLVLLTQALIAAGYIAAGIFSR"
     gene            complement(5810..>6322)
                     /locus_tag="PLR13_09080"
     CDS             complement(5810..>6322)
                     /locus_tag="PLR13_09080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19533.1"
                     /translation="GRGYLSVALASRNPDSHVTGVDYSRMQVRRAERYRREKKIPNCS
                     FRQNNVLSLDFQNAAFDAAVSVGSIKHWPDSRRGLAELYRVCKPGSCLIISETDRDVP
                     DNELRKFVQRFRLPFVPEDLLFWGLRHVVFGQSFSQDQLADAVRLAGFRNIESHKVKA
                     CPYVIVKAWK"
     assembly_gap    6323..6422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6423..6929)
                     /locus_tag="PLR13_09085"
     CDS             complement(<6423..6929)
                     /locus_tag="PLR13_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HQC19534.1"
                     /translation="MEKIISGGQTGADRAGLDFAIDHKMDYGGWVPKGRKTEDGSLPL
                     KYHLQEMPTRDYAMRTLQNVLDSDGTVIVSHGFLTGGSALTREFALKYKKSWLHIDLN
                     ALSLPDAAHMLLHWLEEKEIRVLNIAGPKAGKDPKIYKAVLRLLQEALTDSHVLGSGK
                     KSCVKLQCT"
     gene            complement(7124..8662)
                     /locus_tag="PLR13_09090"
     CDS             complement(7124..8662)
                     /locus_tag="PLR13_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC19535.1"
                     /translation="MKKVVIAGVVIIVLVFSFIGFLHGNNAKPSSPSGAAVSSGMPSG
                     ADTYMLLKNVKFRFAEDIFINVHHMLSITEKIKPYPYVDMNNDQSFSLKIISGHCSLE
                     PPVMETLFNKHVLNYKGAPLKDLKLSTVEMDIDGQKVPMLKTSGMMKLVVWLPFEMIA
                     RVGVDKPTNRMTLEAYKIKSVGLPFVKNLMDMSGVKLSTLLSIEPGRGLSIEKNTMYV
                     DALNITPPPKLVGKLLDAKANVSTNSVDLTLGEEKPANVKYTLLVPDAKNFIYIFQGS
                     LIFGKLLALDANLQLVDANPADYLDFYLKKYLLQLSRSAIRMKPDASLIVNIADYQSD
                     SSPAPAKPAEPAKPTSLSKAAPAKAVSTKPVAGKPAKTPAAKPAASAPAVKQHASINL
                     HLEFDTAQSAVKEQYHSHIKKLADLMKKSPDSRVVIQGHTDNLDKFNNPDNNIKLSQA
                     RADSVRQYLIDKFGINAARIKAIGYGPNQPIADNATLEGQKKNRRVEAVIQTSPQGMV
                     ASTL"
     gene            complement(8863..9285)
                     /locus_tag="PLR13_09095"
     CDS             complement(8863..9285)
                     /locus_tag="PLR13_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQC19536.1"
                     /translation="MMIVTKTIKLQTQGFNDMVNITPRVLSLLEDSPVADGLICVFCP
                     GSTGSVTTIEYESGVLHDLKKALERIAPSDIPYRHDERWGDGNGFSHVRAALMKPSLT
                     IPLVGGRPTLGTWQQIVFIDFDNRKRHRELVVQIIGDA"
     gene            complement(9298..9795)
                     /locus_tag="PLR13_09100"
     CDS             complement(9298..9795)
                     /locus_tag="PLR13_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HQC19537.1"
                     /translation="MDKIFTKKGVLFGFLLVAFIIVFEIILASLSLPAWPAFMVMIFF
                     FVVHEDTKQGAPIVIGGLAGIFCAVLSEMFIDVLMPVMGHEAAKLLFIGLFVYAIVLL
                     KDAIPYVFNSYAFMFFLVSAIARPSATVSPFVWMAVELVVGVIFILGILGINRIVSAI
                     LTEEK"
     gene            10048..10530
                     /locus_tag="PLR13_09105"
     CDS             10048..10530
                     /locus_tag="PLR13_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19538.1"
                     /translation="MKLHSLLLLIILAAIAAFSALNWSTFISPTNLSLGFTSVHMPLG
                     LVMLGMLVLIVMLCLMFVVYIQAKALLESHQHAKALKAQRELADQAELSRFTELRNYL
                     EGQLRAQEAKSSELKTEILARIEQQENHLRTAIEEAGNTLAAYIGEMDDRLGKKTDGG
                     "
     gene            10981..>11439
                     /locus_tag="PLR13_09110"
     CDS             10981..>11439
                     /locus_tag="PLR13_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19539.1"
                     /translation="MKTNLTDHQAKTAYLEQHYGKDILPLSAFEQTMPGSEPIEILFF
                     YKKDTPVSKLRASLLKTIDHYNLFSSRLIMTDRDKFALQYCTDGALAVVLPSLDKKFD
                     EIDIDEIKSRMVHVKTLPGEHLFAVTGIPVRDGIFGGISCSHTVADGISLM"
     assembly_gap    11440..11539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11540..12093
                     /locus_tag="PLR13_09115"
     CDS             <11540..12093
                     /locus_tag="PLR13_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19540.1"
                     /translation="RAKAENEKYSISDNQIITAYLLKKYHSVMMPKTDRIVLRTPVNF
                     RDIHPDIDPLYIGNAQYYCVTVYGKDEIDSLSVYDIAFRLKESISSMRNENFLKELSQ
                     LSAYGIEIKTGLFKNDPPGNMDADILSSNLTHLTDLEAMGVGSDVGSVLSISLAYYKT
                     NFIILKEKSGRIFAEITSMYPLT"
     gene            12218..12382
                     /locus_tag="PLR13_09120"
     CDS             12218..12382
                     /locus_tag="PLR13_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19541.1"
                     /translation="MKNLRGVPPKRKGGEGNISGDTIREPFAYFEVPMEEDVVVKIFA
                     GCPPTGGDAV"
     gene            12581..>13923
                     /locus_tag="PLR13_09125"
     CDS             12581..>13923
                     /locus_tag="PLR13_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HQC19542.1"
                     /translation="MPAPQIILELIERFERNIESYKSNGYNETQVRREFIDPFFEALG
                     WDVNNTAGHAEAYKDVIHEDAVKVSGVTKAPDYSFRIGGQRKFFVEAKKPSVNLKDAP
                     AAAFQVRRYAWSAKLPLSIVTDFEEFAVYDCRIKPDQKDKSDKARITYYSWRQYADKW
                     DEIAAVFSKDVILKGSFDKYAESSKAKRGTSEVDNAFLAEIESWRDALARNIALRNTL
                     SVRELNDAVQRTIDRIIFLRIAEDRGIEDYGSLRSLCNGANIYGRLQELFYKADDRYN
                     SGLFHFRNEKDQAEPPDNLTTKLTIDDKPLKEILTRLYYPDSPYEFSVLPADILGQVY
                     EQFLGKVIRLTSDHRAVVEDKPEVKKAGGVFYTPTYIVDYIVKNTVGKLLEGKNPKTA
                     EKLRILDPACGSGSFLLGAYQCLLDWHLQWYTENDPEKHAKAKRPILYKGYGDTW"
BASE COUNT         3699 a         3240 c         3415 g         3369 t
ORIGIN      
        1 tgcaccagga ctacgaaaac tggctggaaa acctggcgcc ccatgagccc gttgctcatt
       61 accgtcataa ccggacgggc gaggataacg gcgatgcgca tttgaaaaga cagatcatgg
      121 gacgggaggt tgtcgtggcg gtgacaaacg gtcaactgga cttcggaccg tgggaacaga
      181 ttttttacgg cgagttcgac ggaagacgca gaaaacgggt gctggtgaaa atcataggcg
      241 agtaatgcat ctttttgcct ccgttgacag ttgaccgtga cggcggttac cgcgtcttgg
      301 ttcgtcctcc agccttatca aggattcacc gggcatgtga tccgtttcag ccgcagcatt
      361 cttccgggta aaaacggttt atttcatgat gaagatcagg gttccggaag gaatggattt
      421 tttctctacg ttgtccccgt tgagaatgcg cccgtcaggc attcgataga atgtcgatgg
      481 ataattccat tttctgccgg cgatccgggt ggcggatcgc cctttttgaa cgtacccgcc
      541 gtaaatataa cccaccagca aacgggttcc tttgggcgga tttttcagga gcttgcgcag
      601 agacgacgat ttcttcattt ctgcgccggt tcggatcagt ccgtcgggga aaaaatagat
      661 tgttgtcgga tcggcgtaaa gattgccggc cagatcctga accgtgttcc catctttgcc
      721 gatttccagg aatttttcga attcctgctc ccggtcttca tccacaaatg aaacgcgggt
      781 acccacaggg atgctgtccc atttgcggat ttcattgccg cgcaatttct ttccgttggg
      841 ataggtataa attgtcgagg gggcatcgta cttatcccgg gcaatgcgcc aggcattgtt
      901 cccctgggca ataaccatgg atcgactgcc ggatgaatcc ttcgttcctt tcaaagccgc
      961 caacaccttc gggggtttgt agagaaaacg gtgcagatcc ggatcggcaa caacgagccg
     1021 gccggcggca acgtccggat ctcttttggg tccgggcaag agccccagaa gttttctgac
     1081 atcgggatga gaaaaaatca ttccgcattt ttttcggccc cgatgttttc ggggatgaaa
     1141 aatgttcggc cgtccataag ccaccatgga gtgcgtcaga atgtttttat ccttgatccg
     1201 gtaaatcttt ttcaactgac gcaatagttg acggagcgcc gtgtactgcg cccgggtgat
     1261 gtcttgatcg tgaaacccga caacttcaat gccgatggaa acattgtcaa gggtggtttg
     1321 ccggttccac atgcttctgc ccgcatgggt ggcgattttt ttgcggtcaa tgattcgata
     1381 tacttttccg gtgggcgtaa caaaatagtg cgtttccccg aactgatgaa ccttgcgaag
     1441 cgaaccgttc agagcgcctt ctgtcgtatg caaaatgatg tattgggttt tcgaacgcaa
     1501 cggacgtttt ttattcaaag ggctgtacat gttgctaatg tccaaagcat gaacgtccgt
     1561 cgccaaaaac ataaccgcaa aaataacgat gatgaacgat attaaaaatg taaatccgtg
     1621 cgacgttttc tttttaaaaa tcattgattg tccaatggcc atctttatcg attcattgct
     1681 ttgtgctttc ccgaaaaaac gtatcatcca tttattcttc aagagtgccg ctcaatgcga
     1741 tcaacgtcag gggatcgacc cggttgccgt tgattctcac tccccagtga agatgaggac
     1801 ccgtaacgcg tcccgttgca cccaaaaggc cgatgacttc gcccttgtga atatcatctc
     1861 ctttgtttac caatattttg tcaagatgaa aatacatgct gatgatgcct ccgccatggt
     1921 tcacataaac cgatttgccg ctgaaaaaat ggtcggccgc tagaagaacc ctgccgtgat
     1981 tgatggcgac aaccggatcg ccttttttgc cggccaggtc cacaccggta tgcggcgatc
     2041 tgtgcagctg gttaataata cttttcttgc cgaacgtgcc cacgatatct ccgtcaaccg
     2101 gcatcacgaa atgccctttc cattttggcg atgtatcgcc ggccggccat aacgaatgaa
     2161 tgaccgcggc ctcgtttttt actctcttta aggaatgagc atccagttcc actttggttt
     2221 tgggaagttt caatcggcga acgccgtaat cttttaaacg gatacggatc ggatatcttt
     2281 cctgaaagcc ggaagaggtc tcccggatca tgcagtcata taatccggtt tcctgatgca
     2341 ggtccgcgct gataaatccc gaccatgatt cgtccgacgt gctgtatacc aggggtactt
     2401 ttttttgcag ccatgttatt cggggctttt tttgctcgtt cgtctttacc gtgacaagaa
     2461 tcactccgcc ccggtccaac gtgtccgatg aaaaggacac gtccgctttg gacaacgccc
     2521 cggtgtttgc cggaaatgta aaaagcaaaa gcgctgaaaa aaaagataat gcaaatccga
     2581 ccaatgtcct tctcaattgc gttatttcct caaaaagtta ttcctgtgcg ttagacagcg
     2641 caaaacggga taaaaaaaat cgctcacccg acttatgcca gatggcgacc caaccatcga
     2701 aaaatctcct caagtcctga tgactttttg gaccccattc gatatttttc tttttaccta
     2761 tcatgtatgt gataaaaatg caagttgcga tagccgttta cactgacaaa atcaaaaata
     2821 ttcttcggtg ctacttgttt aaaaagtgtg gaagggttta ggcgcccggt gtattttctt
     2881 ccttgcatcc tcttttaaaa ccgaacaggt tttttgattg acattattta taaaaaatag
     2941 aaatcttaaa ctgatgatgg aaaataaaaa agatagcatt ccggaccttg cggaatgcga
     3001 agacctgatg gtccggtatg ccatgttgcc gaacattgtg gagcattcca gacaggtaat
     3061 gcgggtttcc cttgccatta cggaccatct tcaggaggga ctgtcgataa atcgggattt
     3121 gattgttgcc gcagcgctgc tgcatgacat taccaaaaca aggtctcttc aaaccgggga
     3181 aaaacacgct gactccggag gggtgctttt gcgaaaactg ggatttcgac gtgttgccga
     3241 gattgtggag cagcatgtca cgataccgga tctgaatctt cagggcggtc tggaagaaag
     3301 ggaaatcgtt tattatgccg acaagcgggt gatgcacaac gttattgtcg gcctcgatga
     3361 gcgggtggat gatctgatta agcgatatgc caccgacgag aaaatccgcc gccggattct
     3421 tctcaataaa aatcaggcgc tggccgtcga acggaaaatt tccggtttta tgaaaatgga
     3481 tatccatcag gccatcggaa aacttgttgc acatgatcgg gataaaccgt aaaatcatca
     3541 gggaaaacag acgcggaaat aatctatgga aagaaaaaaa ctgaccatca ccatccagca
     3601 aggtgaaaac gtttcggcag ttctgactct gccgccggat aagaagaaac actaccgcgc
     3661 cggcgtgatc gttgcacacg gtgccggaaa cgatatggaa catgaaatgc ttgtcgattt
     3721 cagtgacggg cttgcccggg cggggtatcc gtcgctgcgt ttcaattttc cctataaaga
     3781 aaaaggcctg aaggcgccgg acggtcaaaa aaaactggaa gcgacctggg gcgcggtatt
     3841 tctgcatttc cagaccgata tgccttttca ggtcgatcat gtcattgcgt ccggaaaatc
     3901 gatgggcgga agagtggcct ctcaaatggc cgcagaaaaa caattgccgg ccgacgggct
     3961 tatttttctg ggatatcctc tgcatcctgc cggcgacctg accaaaacaa gagatgcgca
     4021 tctgtaccgc ctggacgtgc cgctgctttt ttttgcgggg acgaaagatg cattgtgcga
     4081 tatgattctg ttcaggaaag ttctgaaaaa tctgtctgca ccttggcaac tggatgtgat
     4141 cgaaggcggc gatcattctt ttcatgttcc gaaatccctg ggcatccgtc aatcggcaat
     4201 tcatgaccgc attgtaaaaa catccggagc gtggctggat ggacaattct gaaacccggc
     4261 acattaaaag gagataagga ggaaaccatg gaaaatatat ttgataagat caagtccaat
     4321 ttgaaaaaag gcatggaaga ggggattgcc gtcttgcggg aaggcgcaac cgtcgtgtcc
     4381 gtgagaatga atgaactttc ggaggaagga aaaagacaat acaaaatttt caatcttaac
     4441 ctgaagatac aggagcaaat taaggaactg ggctgtatgg cttatgcggc gcttgataaa
     4501 gggaaacgtc tggacgacga taaaaaaata aaggcggcgt attcgaaaat caaaaagatc
     4561 gaatggcaac tgaccaaaac cgaagggcag aaaaaagtga aaacaccttc cgcgggaaag
     4621 acggcggcca aaccaggcgc gaaacgcgcc aaagcgtcac cgaaaaaggc caaaccgcag
     4681 tccgccaagg cggccggtaa atcgtcggct ccggggaccg ggccgaaggc aacatccgga
     4741 tgaccaggtg ctttaaaggg ttgatccgat ttaacgagag aagatgccgg cggcaatata
     4801 ccctgccgca atgagcgcct gggtcagcag cacaagcagc acgttttttt tcatggtgtc
     4861 catcaggagt tctctttcgt cataacgaaa agaacctcta ataacctgaa gggccaacgg
     4921 catcgtgaga aaacacacaa gtgtaaagac gggcatagac ccccaaatga ctgcgcccag
     4981 gacccagata tagaccagca gagtgacacc cgagaaaaag aaggcggcgt ttttctttcc
     5041 ggcaacgatg ggcagtgtcc gtcttttcac cgtgacatcc gcttcggcgt cgggaaattc
     5101 attgagcaga agcagattgt gcaccagaaa atacgacggc atggaggcaa ccagcgcggt
     5161 cggagcatat tgtccggtgt gtacaaaata agcgcccatc acaggcagaa tgcccagtcc
     5221 caatccgggc gaccattcag gataacccat ttttaaaata aacggcgtgt ataaaacaat
     5281 caacaacatg gctgcaatca gcagcgggag aagcatccat ccgcttttca tgatgaagaa
     5341 gacggcaatc ggtacaagga cgacaaagca gataatcccc agttgaaggc tttcatgtac
     5401 cgtcagtaat ccggtcggaa cggcgccgct gccgccgctg aaaggcgtgc ggttggtttt
     5461 ataatccagg ccgctgcggg catcgaaata atcgttgaaa atattgaccg ctgcgtgggc
     5521 aatcagcaac ccgatggtgg caagaaccgc atagcagagg ttgaacggcg agccgtaccg
     5581 gacggattca taccaggcga cggcagcgcc gagaatgccc agtatcagag ccagaatcag
     5641 aaagggaata cggacaaccg tgagaatacc ttttaacgtc atggaatatt tttcatcctt
     5701 tgccgaaatg attcggattg tcgtttaaca aaatattgat cgatctggca agcatataat
     5761 gaaggccgga ggaatggatg tgaaaatcag ccaggaaggg ttattggttt tatttccatg
     5821 cttttacaat gacgtaggga caggctttta ccttgtggct ttcgatattg cggaatccgg
     5881 ccagacggac ggcgtcggcc agttgatcct gagaaaagct ctgtccgaaa accacgtgac
     5941 gcagtcccca gaaaagcaga tcttccggca caaaaggcag gcgaaagcgt tgaacaaatt
     6001 tgcgcagctc gttgtccggg acgtcgcggt cggtttcgga aatgatcagg cagcttccgg
     6061 gtttgcagac gcggtaaagt tctgccagac cccggcggga atccggccag tgtttgatcg
     6121 atccgacact caccgccgca tcgaaggcgg cgttctgaaa atccagagac aggacattgt
     6181 tctggcggaa agagcagttc ggtatttttt tctcccggcg gtagcgttca gcccggcgaa
     6241 cctgcatccg ggagtaatcg actcctgtaa catggctatc cggattccgg gatgccagcg
     6301 ccacagacag gtatccccgg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nncgtgcatt gaagcttgac gcatgacttt ttgccggagc ccaaaacgtg ggaatccgtc
     6481 agcgcctcct gaagcagccg cagaaccgcc ttatatattt tcgggtcttt gccggctttg
     6541 ggaccggcga tattgagcac gcggatttcc ttttcctcca gccagtgtaa aagcatatgg
     6601 gcggcatccg gcagagataa tgcgttcaga tcaatgtgaa gccaggactt tttgtatttt
     6661 agggcgaatt ctctggttaa tgccgaaccg ccggtgagaa aaccgtgaga cacaatgacc
     6721 gtgccgtcgc tgtcgagcac attctgaagg gttcgcatgg cgtaatcgcg tgtcggcatt
     6781 tcctgaagat ggtatttcag cggaagcgaa ccgtcttccg tttttctgcc tttgggaacc
     6841 catccgccat agtccatttt atgatcgatg gcaaaatcga gtcccgctct gtccgctccc
     6901 gtttgcccgc cggatattat cttttccaac ctgatcatcg attttcaacc cgactctttt
     6961 tgagtaattt aaatcatttt taccacatgt caaaggggcg ttcggtttat tatgctcgag
     7021 caacaaacca aaatacggtc atgtgaacgg ggattaccgg taaagaaaaa cagcgacagg
     7081 gcgtatccgg tggatagcct tgtcgctgtt ttatgaaacg gggctaaagg gttgatgcga
     7141 ccatcccctg cggcgatgtt tgaataacgg cctcgaccct ccggtttttc ttctgtcctt
     7201 ccagagtggc attatcggca atcggttggt tcggtccgta gccgatagcc ttgatgcgtg
     7261 cggcattgat gccgaatttg tcgatcagat actgacgaac actgtccgcc cgggcctgag
     7321 acaattttat attattgtcg gggttattga atttgtcgag attatcggta tgcccctgta
     7381 tgacgacgcg agaatccggt gattttttca ttaaatcggc cagttttttg atatgagagt
     7441 gatattgttc tttaacagcg gattgagccg tatcgaattc cagatggaga ttgatcgaag
     7501 cgtgctgctt gaccgcgggc gccgaagcag cgggtttagc agcgggcgtt ttggccggtt
     7561 ttcccgcaac gggttttgtc gaaaccgcct tggccggagc ggccttggat aacgatgtgg
     7621 gcttggccgg ttccgctggt tttgccggag cgggcgatga atcggattga taatcggcaa
     7681 tattgactat cagcgaagcg tccggcttca tccgaatggc acttctgctt aattgtaata
     7741 aatatttttt cagatagaaa tccagataat cggccggatt ggcatcaacc agttgcaggt
     7801 ttgcatcaag cgcaagcagt tttccgaaga tcagtgaccc ctggaagata tagataaagt
     7861 tttttgcgtc cgggaccaga agggtatatt tgacgtttgc cggtttttcc tctccaagtg
     7921 tcagatcgac ggagtttgta ctgacgtttg ccttcgcatc aagaagcttg ccgaccagtt
     7981 tcggcggcgg ggtgatgttc agcgcgtcga catacatggt gtttttttca atggagagac
     8041 cccggccggg ttcgatggat aaaagggtgg aaagtttgac gccggacata tccatcaggt
     8101 ttttgacaaa tggcagcccc acggatttta ttttataggc ttccagtgtc atccggttgg
     8161 tcggcttgtc cacgccgact ctggcaatca tttcaaacgg aagccagacg accagtttca
     8221 tcatgccgct tgttttcagc atgggcactt tctgaccgtc aatatccatt tccaccgtcg
     8281 acagcttaag atctttcagc ggtgcgcctt tataattcaa aacgtgttta ttgaagagag
     8341 tttccatcac cggcggctcg agcgagcaat gtccgctgat aattttaagg ctgaaggatt
     8401 gatcattatt catatccacg tacgggtaag gtttgatctt ttcggtgatc gacagcatgt
     8461 ggtgaacgtt gatgaaaata tcttcggcaa atctgaattt gacatttttc agaagcatgt
     8521 aggtatcagc tccggacggc atgcctgacg ataccgccgc gccggaaggt gatgaaggct
     8581 ttgcattgtt tccatgaagg aaaccgataa atgaaaaaac aagaacaatg atgacgacac
     8641 cggctatgac aacttttttc ataggcaccc cctaaaagca aaagaatagg aaattaatca
     8701 aaaaacggtg aaactttgat ttctgaggtt tgcagcctac cacaaaaaca aaaaattcaa
     8761 aacagagaat tcgaggcggc tccctgcaca aagaaactcg tgaaagcaaa tcgggaatgt
     8821 ctgtcaaacg gtcgcatcgg gcacgatgca ggtcgaatcg aattatgcat ccccgataat
     8881 ctgcacaacc agttcccggt gtcgttttcg gttgtcaaag tcgataaaga caatctgctg
     8941 ccaggttccc agcgtcggcc tgccgccgac cagcggaatc gtcagagacg gtttcatcag
     9001 tgcggcgcgg acatgcgaaa agccgttgcc gtctccccat cgttcatcgt gccggtaagg
     9061 gatgtccgac ggggcaattc tttcgagtgc ctttttcaaa tcatgcagta cgccggattc
     9121 atactcaatg gtcgtcaccg aaccggtgga gccggggcag aaaacgcaaa tcagcccgtc
     9181 ggcaaccggc gagtcttcca gaagggaaag aacccgggga gtgatattga ccatatcgtt
     9241 gaacccctgg gtttgaagtt taattgtttt tgtaacaatc atcatgcaac gagcttttta
     9301 tttttcttcc gtcaaaatcg cggagacgat ccggttgatc cccagaatgc ccagaataaa
     9361 tataacgccg acaacgagtt cgacggccat ccaaacaaag ggcgaaaccg ttgccgacgg
     9421 ccgggcaatc gcggagacga gaaaaaacat gaaggcgtag ctgttgaaaa cgtaaggaat
     9481 ggcgtccttc aaaagcacaa tcgcatagac gaacaggccg atgaaaagaa gtttggcggc
     9541 ttcatgtccc ataacgggca tcaggacgtc aatgaacatt tcactcagaa ccgcgcaaaa
     9601 gataccggcc agtccgccga tcacgatagg agccccctgt tttgtgtctt cgtgtaccac
     9661 aaaaaagaaa atcatcacca tgaaggccgg ccaggcggga agagaaaggg aggccagaat
     9721 gatttcaaaa acgatgatga aagccaccag aagaaaaccg aacaaaacgc ctttttttgt
     9781 aaatatttta tccatgtatc cctaccgaat ccttaatgtt ttgaggaaga agaataagga
     9841 attcaacaga atttgtcaag cagagaggaa agatgcggaa tagaattgat tttgaaaaaa
     9901 gtaattttat gctaaatgaa agatgaacgg gctattacca atctccagcg aagaaataga
     9961 catttttttt atagagaaat ggaagattag agaatcccgg taaacatttt cagcatattt
    10021 caaaataaat cagtggaggc attggatatg aaactacatt ctttgttgtt attgattatc
    10081 ctggcggcga ttgcagcgtt ttccgcctta aactggagca cttttatttc gccgacaaac
    10141 ttatcgctcg gtttcacctc ggtgcacatg ccgctggggc tggtaatgct gggcatgctg
    10201 gttttgatcg tcatgctatg cctgatgttt gtcgtttata tccaggccaa agcgcttctg
    10261 gaatcccatc agcacgccaa ggccttgaag gctcagcggg agcttgcgga tcaggcggaa
    10321 ttatcgcgtt ttaccgaatt gcgaaactac ctggagggcc agctgcgggc acaggaagcc
    10381 aaaagttcgg aattaaaaac ggaaattctt gcgcgcatcg agcagcagga aaaccatctg
    10441 cgcaccgcaa tcgaagaagc cggaaatacg cttgcagcct acatcggaga aatggatgat
    10501 cgcttgggga aaaaaacaga cggcggctga tgacgggtgt ttgccatgca gacacagccg
    10561 gaaatgtaag acgataaaaa aacaacctct tcctttcggg aagaggttgt taaaggatca
    10621 attaatagtt cggataatta atcacagccc ggtttcacca ccgctagggg gacaacattc
    10681 tggaattatc atgtcttttc tcctttgatc ttgaatgaga ttggaattta aatctccgtg
    10741 tgtgcgtgaa tttacgttcc gcctgcaggg ggaccgcttt ctaaaatttc tatagttctt
    10801 ctttattaat tgtgaaaatt ttagttatta taattatttt aaaaaattta ttcaagacta
    10861 aaaattgttg tgcatttcag tttcgtttat gtttaaaata ccttcatgcc gagaagtcat
    10921 aaaggttcgg attaagatga cgatattcaa aaatagagtt tcccgacggg ggtgaacctt
    10981 ttgaaaacca atttgaccga tcatcaggca aaaacggcct atctggaaca gcattacggc
    11041 aaagacattc tgccgctgtc ggcattcgag caaaccatgc cgggatcgga gccgatagaa
    11101 attctctttt tctataagaa agatacgccc gtatcaaaac tgcgcgcgag ccttttgaaa
    11161 accattgatc attacaatct tttttccagc cgcctgatca tgaccgatcg ggataaattt
    11221 gcgttgcagt actgcacgga cggcgccttg gccgtcgttc tgccgtctct ggataaaaaa
    11281 ttcgacgaga ttgatatcga cgaaatcaag agcagaatgg tgcatgtcaa aacgttgccg
    11341 ggcgagcatc tgttcgccgt aacgggcata cccgtccggg acggcatatt cggcgggatc
    11401 agttgttcgc ataccgtggc cgacggcatt tccttgatgn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnna cagagcaaag gcggaaaacg aaaaatacag tatttccgac
    11581 aaccagatta tcacggctta tttgctgaaa aaatatcaca gcgtcatgat gccgaaaacg
    11641 gaccggatcg tgttgagaac gcccgtgaat ttccgcgaca ttcatccgga tatcgatccg
    11701 ctttatatcg gcaatgcgca gtattattgc gtgaccgttt acggaaagga tgaaatcgat
    11761 agcctgtcgg tttacgacat cgccttccgc ctgaaagaat ccatttccag catgcgcaat
    11821 gaaaattttc tgaaagaact atcccaactc tcggcatacg gcatagaaat caaaaccggc
    11881 ctgttcaaaa acgatcctcc gggcaatatg gacgcggaca ttctttccag caatctgacg
    11941 caccttaccg atctcgaagc catgggcgtc ggttcggatg tcggcagcgt tttatccatc
    12001 agtctggctt attacaaaac aaactttatt atcctgaaag aaaaaagcgg ccggatcttt
    12061 gctgaaataa caagcatgta tcccctgacg tgaaaggtat tgaagaatct ttcgggcata
    12121 agactaccgg caatttgtta ttgataaaac attaaaaatc ttttagtatt cataaccgga
    12181 agaagtgaac aacaatttaa taaaaaagga gaagattatg aaaaatttaa gaggtgttcc
    12241 acccaaaaga aaaggcggtg aagggaatat cagcggcgat acgattcgtg aaccttttgc
    12301 ttactttgaa gttccgatgg aagaagatgt tgtggtaaaa atctttgccg gctgtccccc
    12361 cactggtggt gacgcagttt aacaatctct ttatgttaaa ataaaaaagg cagaatcata
    12421 aacgattctg cctttttttg ttagtatgac gatgtccgca atcctttgtc gtccgtttat
    12481 atcaaccttc aggaagggga tcggggttga cagttttact ttcagagagt ataaaaccac
    12541 ttcaatcaaa ataatatcca atgttaacaa gggacctctg atgcctgcac cgcagatcat
    12601 tctggaacta attgaacgat tcgaacgcaa cattgaatcc tataaatcca acgggtataa
    12661 cgaaacgcag gttcgccgcg aattcatcga tcccttcttt gaagcactgg gctgggatgt
    12721 caacaatacg gcaggacatg ccgaagccta caaggatgtc atccacgaag acgccgtcaa
    12781 agtcagcggc gtcaccaaag cgcccgacta ttcctttcgc atcggcggac agcgcaaatt
    12841 ctttgtcgaa gccaagaaac catccgtcaa cctcaaggac gcgcccgccg ccgcctttca
    12901 ggtaagacgt tacgcctggt cggccaaact gccgctttcc atcgtgaccg actttgaaga
    12961 attcgccgtt tacgactgcc gcatcaagcc ggatcaaaaa gataaatccg acaaagcccg
    13021 gatcacttac tactcctggc gccagtacgc cgacaagtgg gacgaaattg ccgccgtctt
    13081 ttccaaggat gtcattctca aaggctcttt cgataaatat gccgaatcca gcaaggccaa
    13141 gcgcggcacg tccgaagtgg ataacgcttt tcttgccgaa atcgaaagct ggcgcgacgc
    13201 gcttgcccgc aacatcgccc tgcgcaacac gctgagtgtt cgagaactca acgatgccgt
    13261 ccagcgcacc atcgaccgca ttatcttcct gcgcatcgct gaagaccggg gcattgaaga
    13321 ctacggcagc ttgcgctctc tttgcaacgg cgcgaatatt tacggccgtc tgcaggagct
    13381 tttctataag gccgacgacc gctacaactc cggcctgttt catttccgca acgaaaaaga
    13441 ccaggccgaa ccgcccgata atctgacgac gaaactcacc atcgatgaca aaccgctcaa
    13501 agaaattctt acgcgccttt actatcccga ttctccttat gagttttcgg ttttgcccgc
    13561 cgatatattg gggcaggtct atgaacagtt tttaggaaag gtcattcgcc tgaccagcga
    13621 tcaccgcgcc gtggtcgagg acaagcccga agtcaaaaaa gcgggtggcg ttttctacac
    13681 gccgacgtat atcgttgatt acatcgtcaa aaatactgtg ggtaaattgt tggaagggaa
    13741 aaatccaaaa acagcagaaa agctccgcat ccttgatccc gcctgcggct ccggctcgtt
    13801 tttgctgggc gcgtatcagt gtctgctcga ctggcatttg cagtggtaca ccgaaaacga
    13861 tcccgaaaaa cacgccaaag ccaaaaggcc gatactctat aaaggatacg gcgacacgtg
    13921 gca
//
LOCUS       DAONQZ010000061        13397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5125_length_13397_cov_14.079673, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000061 DAONQZ010000000
VERSION     DAONQZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_13397_cov_14.079673"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLR13_09130"
     CDS             complement(<1..791)
                     /locus_tag="PLR13_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQC19543.1"
                     /translation="MKKKSKTSQKSILINKKTSSKTKSSSKSSPALKKEASNSSKSKP
                     AKTESKKSFSLDRDIHEVIAREWQTAFDAVTDAICLLDKNQRILRSNRAMQDLFAVSG
                     KKIVGKYCWEIIHGTAKPVPGCPIVKSSRSRKPEKMELKTKGRTFNVTAYPVFDEKNT
                     AIIGFVHIINDVTELKQAWEIVNASEMRLKAQYQGSPLAIFIWQKQDKDFVLIDFNKM
                     AFALSQGLVKKFINKTARQLYKKRPDILKDIQDCFAKKTTIKKEL"
     gene            complement(969..3479)
                     /locus_tag="PLR13_09135"
     CDS             complement(969..3479)
                     /locus_tag="PLR13_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQC19544.1"
                     /translation="MRRPLILLLIAMIAGILAGSYWIIPFYSLCAAAVVTLILILVSL
                     RKKWLPAGFVFIFLAVFLLGFFNIQKAFHFPESRPHILSYANEGKITVEGFVSESPVA
                     YADKNVLIVQPLRILKDKTYRPVFGAVRLSIPSGLTFYLGDFIRFQSSLRKIENFNNP
                     GALNYKRLMNLQGIYISGYIHDASDIILLRKQPSFGVHRMIESFRLYLKRIIGNHAPM
                     PEKSIIEAMTLGNQNEIPSVIRDNFSKTGTSHILSISGLHVGMVAASFFVLIFLILKS
                     SEYLMLKFNIVKIAAGFSFVIVLLYAVIAGMGVTVMRATFMAFVFLLALIFGRQKELY
                     NTLAIAGLMILAVAPEALFDISFQLSFVSVLAIIYITPRLNGLIQHKNLPLPSWLQSP
                     FRYLYMTIMVCIAATLGTLPLTVFYFDRVSLVTIAANLIAVPLLGTLTLALAMFFILS
                     AFFSEVVAGFFVQLAAFATRISVNIINHLADFTWSSVSLSKPSLMEIALFYLLIFFAV
                     QWIDEIRKKKNRLHFSSSRFAFINYALIFIVVMIGANAAYLTLRERISNDLTVTVIDV
                     GQGNSALVQFPKGTNMMIDGGGFSKSSFDVGKGIVAPFLHKQRIRRIETVVLSHPHPD
                     HLLGLIYILNHFNVSQIWKTALPVNPDIYPEWESAVKTNRIKTLLLGVTAGEKNVNGV
                     RIKILWPPDHVDPGGKALSHNDENDLSMVMKITYGHVSFLFPGDISSKVENILVRSGA
                     DLRSDVLVVPHHGSNHSSSSDFIRAVGCRFAVVSAGRSNVFRHPHPSVLQRYRQAGVY
                     IFRTDCDGAVTFKTDGHHLRTETFFQKTADIPEKQQIR"
     gene            3578..5263
                     /locus_tag="PLR13_09140"
     CDS             3578..5263
                     /locus_tag="PLR13_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19545.1"
                     /translation="MTTSNRDESANGKNKRRILSLIISRIAITTLLLAITVFIDTRRQ
                     LFGIPYAAINVFYFIVVVIYLFSSLYIVLYKLKINYLYNLYLQIAIDIVTVTFLIFMF
                     GNTQIDYSLFYTLIIIYSALFLGRKGGLIVASISSIFYGIFLNLEFYKLMPSQSLIKL
                     DYDMNAADALTNLVVRITSFYVLAFLASFIIEQEKKTSSLLEEKESEFNQLDLLFRSI
                     VESVYSGVMTVDLNNIIKTFNTAAEEITGFPRTRVQGLNIQNIFPEFLPYLNKETIDE
                     QAKARIEIIIQGRKKEKINLGVSVSPLIGKSEKQIGNILIFQNITQIKQMEKALEKNK
                     NMALIGEMAAGWAHEVRNPLAAITGSIEMLKQGLNPDGTNKRLMEIVLRGKDQLENFV
                     RNFLMLARTVPAAREMVDINRIIDEIQEALQIDKDWNDTIKIKKILSGDVSVYANKEQ
                     IRQVLNNIINNAVEAMEEGGLLSIGTRVVQTSGKKKYAEIVVSDTGCGIKEEDLNKIF
                     EPFFTKKTRGTGLGLAIVNHLVEGYKGKIEIESTEGKGTRCRLLLPVEKDENL"
     gene            5305..6663
                     /locus_tag="PLR13_09145"
     CDS             5305..6663
                     /locus_tag="PLR13_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC19546.1"
                     /translation="MAKILIVDDDQGMREMLEIMLQKEGYDVTSIGDPVKAVDLCRKK
                     NFDMVITDLKMPKIDGIEFLKSVKDQRPETIVILITAYASGETAINAMKEGAYDYVEK
                     GRSLDELKKVVRAALMNKGLISDDPAEQKCTGATESNHAFCGMIGTSREMLKIFNTIK
                     KVSDTPANILILGESGTGKELVARAIHENSSRSKMPFVAINSGGIPENLLESELFGYM
                     KGSFTGAYADRQGYFEMARGGTIFLDEIGELSPSLQVKLLRVVQEKTFRRIGGAEDIK
                     VDVRIISATNKNLQERVKKGDFREDLYYRLNVIPILIPPLRERREDIPLLIDTFIEKY
                     SKMFGKEIRTISSYAVELLLNYDFPGNIRELQNIIERGVAMESSNIILPESLTFGAPK
                     FDVDISDQGIDLNAELDKIEKTIIEKALQKTKGSKKKAAELLNVTFDSLRHRIEKLGV
                     ES"
     gene            6953..8155
                     /locus_tag="PLR13_09150"
     CDS             6953..8155
                     /locus_tag="PLR13_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQC19547.1"
                     /translation="MKSRILIAILSAVFLIAGTGSLQAKTIKVGAAINLTGPASTWGQ
                     FHEKGQRDYFRYVNEVKGGVYGNKIEMITVDTAYKVPEAQAAVNKFVLQDKVDMIATW
                     GAGEGLAAKPIIQRYKVPAINYSTSWEILEKPVDYMYLPFGSYKMDCHAVFEYIKSIH
                     KGKDAPKVGLLTYNNAYGRSIHAPSKAYAKQLGINLVAVEEFPPKTVDLNTELLRLQK
                     AGAQYIFMQMLPSAIITAFKSADRINYNPVFLGTWTSTDPDFFKMGKGLIRDRLVIQF
                     PGGLPSDKSKGMAVMQELWKRYKTVSKFDASYWEGVVVGMIMERAFMRAYEKSKNINP
                     ATINAAMESFRNEDFGGLVPAITYTKDNHGASFTARMVKVKEDGTYIPLTNFYVPGRD
                     KIKLLGSK"
     gene            8225..9052
                     /locus_tag="PLR13_09155"
     CDS             8225..9052
                     /locus_tag="PLR13_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19548.1"
                     /translation="MKAFRSRPSIMINRREENKKAELAIQNLRLSFGGVVAVKDVDLQ
                     VHTGELMAIIGPNGAGKTSLLNCITGFYRAQQGKIVFNGKEITRLHTHELVGVGIGRT
                     FQNIELFPGMTVLANMTLARHIHCRYSFIKSFLFSKSVRDEEIRHRQVLEEIIDFLEM
                     QSIRKQAVGSLPYGMQKRVELGRALALEPKLLVLDEPFAGMNMEEKEDMVRFLLELNQ
                     QWGLTMILVEHDMSIVMSISQRIMVLNFGEKLGEGNAEEISANPEVIKAYLGESEAM"
     gene            9074..10999
                     /locus_tag="PLR13_09160"
     CDS             9074..10999
                     /locus_tag="PLR13_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQC19549.1"
                     /translation="MSKGTSHITKRNLTLPQVLMENAAVYGDAKTAIREKAYGIWQKY
                     TWADYGRYVRMTAAGLAALNLRRGENLCMVVHNHPEWLFSELAAHVLGATTLNLFTSS
                     IADELAFSIKRINSPVVVVQDQEQVDKLLEIKEKLPQTQKVVYIDPTGMASYQDDPWL
                     ISYAGLLKLGEQYLEEHPGFIDSEINRGRPDDIAVMIQTSGTTGVPKLAMLSHRNLLT
                     MARQWIEETRIKPEENWLSMSPPAWIVDQMWGMGVALDGAMCMNFPETAETVLEDFRE
                     IGPSMIITSSRFWEDMASRIRVKISDAGWLKKTLFYLGEKIGKSVVEKQSHRQPVGWP
                     LQVLHSVMSVIVYRPLLDRLGCSHFRSAFTGGHPISPDVIHFFRAIGLNLKQCYGLTE
                     SCGIFQIQPDHEVKLETVGKPMVLTEVKIAGDQEVLVKSEANFSGYHNDYESTQNAFE
                     NGWLKTGDAGYIDHDGHLLIIGRKEDIIRNKSGEAFSPDFIETRLKFSPYIKEAVTFG
                     EARPYIAAMINIDMGNVGNWAEERMIPYTTYMDLSQQSAVEELILKEVIAVNEQLPEV
                     MKIKKFVLLYKLLDADDEELTRTGKVRRRFVYGLYLKIIEAMYNNLKNVQVTGKVKYR
                     DGRIGEIETTINVINVN"
     gene            11014..11892
                     /locus_tag="PLR13_09165"
     CDS             11014..11892
                     /locus_tag="PLR13_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC19550.1"
                     /translation="MEFFLQLLVNGVCIGLLYGISAMGFVMIFKSSSVLNFAHGELLA
                     LGAFFFLSLVTWAQLPVYVAFILTMIGCFILGFLIERFFLRPLIGEKLIFVVMLTVGL
                     AAMFKGFILLVWGGNLHTYPEFLPPSMAMQFGSIYVAPAYSATLIISVVFLVLFGLFF
                     KKSSQGIYMRSVADNQKAALSLGVHVRRVFALSWAIAALVACMSGIVLGIINGVNVHD
                     LSSIGLKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIVLVFIL
                     MVKPYGLFGLVEIERV"
     gene            11905..12975
                     /locus_tag="PLR13_09170"
     CDS             11905..12975
                     /locus_tag="PLR13_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQC19551.1"
                     /translation="MKKLSFHPCGNYRETYEQELNIFETDFGRFWMIVFLGVLFFVAP
                     FVLSPYMLYILNTIGIAAIAAIGLNILIGYTGQISLGHGAFFGVGAYAAAILATRLGV
                     GFYIAIPAAGIITALVGMIFGIPSGRLKGLYLTIATLAGQFIIEYVLIQWESLTKGTM
                     GITLPSPEIFGWQITGDRAFFFLVFISLICMTWFAVNIIRSKYGRAFIAIRDNDRAAE
                     GMGIPIFKYKLLSFGISSFYAGFAGALWAYYMVSITTEPFNLGLSVEYIAMVIIGGMG
                     NIPGAIFGATFITLLNELLRFLTGVLMNVPAITSLGLNMAPLKEFVFGLAIVLFILIE
                     PKGLAEIWRIIRSSFRLWPFSY"
     gene            13219..>13397
                     /locus_tag="PLR13_09175"
     CDS             13219..>13397
                     /locus_tag="PLR13_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC19552.1"
                     /translation="MAECLLQLNNISVVYSDVIQVLKGVSLSVEKGHIVSLLGSNGAG
                     KTTTLKAISGLLKPE"
BASE COUNT         3586 a         2908 c         3426 g         3477 t
ORIGIN      
        1 aataattctt ttttgatggt tgtttttttc gcaaagcagt cctgtatgtc ctttaaaata
       61 tccggtcgtt ttttgtataa ctggcgcgct gtcttgttga taaatttttt aaccagaccc
      121 tgggagaggg cgaaggccat tttattgaaa tcgatcagga cgaagtcttt atcctgtttc
      181 tgccagatga atattgccag ggggcttccc tgatattgcg ctttaagccg catttcactg
      241 gcattgacaa tttcccaggc ctgtttgagt tcggtaacgt cgttaataat atggacaaag
      301 ccgatgatgg cggtattttt ttcatcgaaa acaggataag cggtgacgtt aaaggttcgt
      361 cctttggtct taagctccat cttttccggt ttccgggacc tcgacgattt gacgatcggg
      421 catcccggaa ccggttttgc cgtcccgtga ataatttccc agcagtattt cccgacgatt
      481 ttttttccgg atacggcaaa caggtcctgc atggcccgat tggaacgaag gatacgctgg
      541 tttttgtcaa gcagacagat ggcatcggtg accgcatcaa aggccgtttg ccattcacgg
      601 gcgatgactt catgaatatc ccggtcgaga gaaaaagatt ttttcgattc tgttttggcc
      661 ggtttgcttt ttgaggagtt tgatgcctcc ttttttaaag ccggcgaaga tttggatgag
      721 gattttgttt tgctgctcgt ttttttgttt ataaggatgc ttttttgtga ggtctttgat
      781 ttttttttca tggcgtaata tcccttttgt cgattgtgta atgtgaaggt ttttcagcaa
      841 agaacgccgc cggcacaatg tgatcaaaga acgggtttcc tcaaggtcct aaaaaagaaa
      901 gcactaacaa aaaacgggat aagtacatca tttacttgaa aataattata ggctgataat
      961 ttataaagtc aacggatttg ttgtttttcg ggaatgtcgg cagtcttttg aaaaaaggtt
     1021 tccgtgcgca gatgatgtcc gtcagtttta aaggttaccg caccgtcgca atcggtgcga
     1081 aaaatataca ccccggcttg ccggtatctc tgcaaaacgg aaggatgagg atgcctgaag
     1141 acgttcgatc gtcccgcgct gacaacggca aaacggcagc cgacggcccg gatgaaatcg
     1201 ctgctgctgg aatgattgga tccgtgatgc ggcacaacca ggacatcgct tcttaaatcg
     1261 gcgccggagc ggacaagaat attttccacc ttcgaagata tgtcgccggg gaacaaaaaa
     1321 ctgacatggc cgtaagttat tttcatcacc atcgataaat cgttttcgtc gttatgggaa
     1381 agcgccttcc cgcctggatc gacatgatcg gggggccaga gaattttaat ccggacgccg
     1441 tttacgtttt tctcgccggc ggttactcca agcaaaaggg ttttgatccg gtttgtttta
     1501 acggcgctct cccattccgg gtagatgtcc ggatttacgg gtaatgccgt tttccagatt
     1561 tgtgagacgt tgaagtggtt cagaatataa atcagtccca gcaggtgatc gggatgcgga
     1621 tggctcagaa cgaccgtttc aatgcgccgt attctttgct tgtgcagaaa aggtgcgaca
     1681 atgccttttc caacgtcgaa agaacttttg gaaaaaccgc cgccgtcaat catcatgttt
     1741 gtacctttcg gaaactgcac aagtgcggag ttgccctgtc cgacgtcgat gaccgttacg
     1801 gtcaggtcat tggagatcct ctcgcggagt gtcagataag ctgcgttggc gccgatcatg
     1861 acaacaatga aaatcaaggc ataattgatg aaggcaaagc ggctggatga aaagtgtaaa
     1921 cgattttttt tcttcctgat ttcgtcaatc cattgcaccg caaaaaaaat caataagtaa
     1981 aataaggcga tttccatcag cgacggttta gacagggaaa ccgacgacca ggtaaaatcg
     2041 gcaagatgat tgatgatgtt gactgaaatc cgggtggcga aggccgccag ttggacaaag
     2101 aatccggcaa cgacctcgga aaaaaaggcg ctcagaataa aaaacatggc caaggcgagg
     2161 gtcagggtgc ccagaagagg aacggcaata agattagccg caatcgtcac cagcgacaca
     2221 cggtcgaaat aaaatacggt cagaggaagc gtgcccagtg tggcggcaat acagaccata
     2281 atggtcatgt agagataccg gaagggactt tgcagccatg acggaagcgg gaggttttta
     2341 tgctgaatca atccgttcag tcgcggcgtg atatagatga tggccagaac ggaaacgaag
     2401 gaaagctgaa atgaaatatc aaaaagcgct tcgggcgcaa ccgccagaat catcagaccg
     2461 gcaatcgcca aggtattgta aagctctttt tgccttccga agatcagcgc gagcagaaaa
     2521 acgaatgcca tgaacgtcgc ccgcattacc gtaaccccca tgcccgcgat aacggcatac
     2581 agcaggacga tcacgaaaga aaatcctgcc gcaattttga cgatgttgaa tttgagcatc
     2641 aggtattccg atgattttaa aatcagaaaa atcagcacaa aaaaagaagc ggccaccatg
     2701 ccgacgtgca ggccggaaat ggaaagaata tgcgacgttc cggtcttgct gaaattgtcc
     2761 ctgataacgg acgggatttc attttgattg ccgagcgtca tcgcttcgat aatgcttttt
     2821 tccggcatcg gggcatggtt gccgatgatg cgtttgagat aaagccggaa ggattcgatc
     2881 attctatgaa cgccgaacga aggttgtttt ctcaacagaa tgatatcaga ggcgtcgtgg
     2941 atatatccgg aaatataaat gccctgcagg ttcatcaggc gcttgtagtt caacgctccc
     3001 ggattgttga aattctctat tttccttaaa gacgactgaa aacggatgaa gtctcctaag
     3061 taaaaggtca ggccggaggg aatggacaga cggacagccc cgaagaccgg acgatatgtt
     3121 ttatctttta gaatgcgcag aggttgcacg atgagaacat ttttatcggc ataggcgacg
     3181 ggactttcag aaacaaagcc ttcaacggtg atttttccct cgttcgcata agaaagaatg
     3241 tggggccggc tttcgggaaa gtgaaacgcc ttttggatgt tgaaaaagcc caaaagaaag
     3301 acggccagaa agataaagac aaaaccggcc ggcagccatt ttttccggag actgaccagg
     3361 ataaggatta gggtgacaac ggcagcggcg cacaagctgt aaaacggaat gatccaataa
     3421 ctgccggcaa ggatgcccgc aatcatggcg atcaagagaa gaatcaacgg tcggcgcata
     3481 atcgtttttg actttcaacc gcatgcatga aatatattag cgaaaagtgc taaaatatta
     3541 taatatattt cgcaaatgtt ttttttgagg aatctgcatg acgacatcga atagggacga
     3601 gtccgcaaac gggaagaata aacgcaggat tttatcgtta atcatctcca ggatagcgat
     3661 taccacgctt ctgcttgcaa ttactgtttt tatcgatacc cgtaggcagt tgttcggcat
     3721 cccctatgcc gcgattaacg ttttttactt catcgttgtt gttatttatt tattttcctc
     3781 tctgtacatc gttttgtaca aactcaagat caattacctc tataatttat acctgcagat
     3841 agcgatcgat attgttaccg ttacgtttct gattttcatg ttcggcaaca cccagattga
     3901 ttactccctt ttttatacgc tgatcattat ttattcagcg ctctttctgg gacggaaagg
     3961 aggcctgatt gtcgcctcca tttccagtat attttacggg atatttctga acctcgagtt
     4021 ttataaattg atgccgagcc agtctctcat taaactggat tacgacatga acgcggccga
     4081 tgcgctgacc aaccttgtcg tgcgcatcac ttctttttat gttctggctt ttctggccag
     4141 cttcattatc gaacaggaaa aaaagacctc cagcctgctg gaggaaaagg aatcggaatt
     4201 caatcaactg gatttgcttt tccgaagcat tgtggaatcg gtttacagcg gggtcatgac
     4261 ggtggatctg aataacatca tcaaaacatt caatacggcg gctgaagaaa tcaccgggtt
     4321 tccccgcacc agggttcagg ggctcaatat tcaaaacatc tttcccgaat ttctgcccta
     4381 tctgaacaag gaaaccattg acgaacaagc caaggcgaga attgaaatca ttattcaagg
     4441 cagaaaaaaa gaaaaaatca atctgggtgt gtccgtatcc ccgctgatcg gaaaatccga
     4501 aaaacagata ggcaatatcc tgattttcca aaatattacg caaatcaagc aaatggaaaa
     4561 ggctctggag aaaaacaaaa atatggcttt aatcggggag atggccgcag gctgggcgca
     4621 tgaggtcaga aatccgctgg cggcgattac cggttcaatt gagatgttga agcagggact
     4681 gaatccggac ggaaccaata agcgcctcat ggaaattgtc ctgcggggca aggatcaact
     4741 ggaaaatttt gtgcggaatt ttttgatgct ggccaggacc gttccggcag cccgggaaat
     4801 ggtggatatc aaccggatca tcgatgagat tcaggaagct ctccagattg acaaggactg
     4861 gaacgacaca ataaaaatca aaaaaatatt atccggagac gtcagcgttt atgccaacaa
     4921 agagcaaata agacaagttt tgaataacat catcaacaat gcagtcgagg ccatggaaga
     4981 gggcggtttg ctgtccattg gaacccgagt ggtccagacg tcgggtaaga aaaaatatgc
     5041 ggaaattgtc gtatccgata ccggatgcgg tatcaaagaa gaggatttaa ataaaatatt
     5101 cgagcctttc tttaccaaaa aaacaagggg gaccggttta gggctggcca tcgtcaatca
     5161 tctggttgaa ggctataagg gaaaaattga aatagaaagc acggaaggca aaggaacccg
     5221 gtgccgttta ctgttgccgg ttgaaaagga tgaaaatttg tgatttagaa tttgacaatg
     5281 cgttgttgag acaatgaggt cgatatggca aagattttga ttgttgatga tgatcagggc
     5341 atgagggaaa tgcttgaaat catgctgcag aaagaagggt atgatgtcac cagcatcggc
     5401 gatcccgtga aagcagtcga tttatgccgg aaaaaaaatt tcgatatggt gatcaccgat
     5461 ttaaagatgc ctaagatcga cggaattgaa tttctcaaaa gcgtcaagga tcagaggccg
     5521 gagaccatcg ttattctcat caccgcctat gcttccggag agacggcgat caacgcgatg
     5581 aaagaaggcg cgtacgatta tgtggaaaag ggacgcagtc tcgacgaact caaaaaggtt
     5641 gtgcgtgccg cgttgatgaa taaaggcctg atcagcgacg atcccgcgga gcaaaaatgc
     5701 accggcgcca cggaatcaaa ccatgccttt tgcggcatga tcggcacaag ccgggaaatg
     5761 ctcaaaatat tcaacacgat taaaaaggtt tccgacacgc cggcgaacat tctgattctc
     5821 ggggaaagcg ggacgggcaa ggaactggtt gcccgcgcca ttcatgaaaa ttcttcccgg
     5881 tctaaaatgc cgtttgtcgc gatcaacagc ggcggcatac cggaaaatct tctggaaagc
     5941 gagctcttcg gatacatgaa agggtctttt acgggagctt atgccgaccg gcaggggtat
     6001 tttgaaatgg cccgcggcgg aacgattttt ctcgatgaaa tcggagaact atcgccgtct
     6061 ctgcaggtca aattgctgcg ggtggttcag gaaaagacat tccgtcgcat cggcggtgcg
     6121 gaagacatta aagtggatgt gcgcattatt tccgcgacca ataaaaatct tcaggaaagg
     6181 gtgaagaaag gggactttcg cgaagacctt tattatcgcc tgaacgttat accgatcttg
     6241 atccctccgc tgagggaaag acgggaggat atcccgctgc tgattgatac cttcatcgag
     6301 aaatactcaa aaatgttcgg gaaagaaatc agaaccattt cttcgtacgc ggtggagctt
     6361 ttattgaatt acgactttcc gggcaatatc cgcgaactgc agaatatcat cgaacgcggc
     6421 gttgccatgg aaagctccaa cattattctg ccggaaagcc tgactttcgg agccccaaaa
     6481 ttcgatgtgg atatttcaga ccagggcatt gatttgaacg cggaactgga caaaatcgag
     6541 aaaacaatca ttgaaaaggc attgcagaaa acaaagggat cgaagaagaa agccgccgag
     6601 cttttaaatg ttacgtttga ttccctgcgg catcgtatcg aaaaactggg tgtggaatcg
     6661 taaatggtgg tattaagaaa aattataccg aaaccttctt tttgcttgac ttttcaatag
     6721 cgtttgctct atatgactcc acattgggag tttaatgaat atgttaagga tttatttttt
     6781 taaaaaggca gtcttttttt tcttttttag ctttatacgt aaagtctgcc tgatagtatc
     6841 atgaccatcc gtaagtggta atgaaccgtg ggcagataaa aagcccacgg tttttttttg
     6901 ccgtcatcag gacggccgtt ttaaaaaaag aaagaagggg aggggaataa aaatgaaaag
     6961 cagaatttta atagcgattt tatcagcagt ttttttgatc gccggtaccg gaagcttgca
     7021 ggccaaaacc atcaaggtcg gagcggcgat caatttgacc ggtccggctt caacctgggg
     7081 gcagtttcac gaaaagggac agcgcgatta tttccgttac gtcaacgagg tgaaaggcgg
     7141 cgtttatgga aacaaaattg aaatgatcac ggtggatacc gcttacaaag taccggaagc
     7201 gcaggccgcg gtaaataaat tcgttctgca ggacaaagtg gatatgattg ccacatgggg
     7261 agccggcgaa ggcctggcgg ctaagccgat catccagaga tacaaggttc cagccatcaa
     7321 ttattccacc agttgggaga ttttggagaa acccgttgat tatatgtacc tgccgttcgg
     7381 cagttacaag atggattgcc atgcggtttt tgagtatatt aagtccattc ataaagggaa
     7441 agatgctccc aaagtcggtc ttctaaccta caacaacgcc tacggccgtt ccatccatgc
     7501 gccgagcaaa gcatacgcga aacaactggg catcaatctg gtcgccgttg aggaatttcc
     7561 tccgaaaacg gttgatctga atacggaact tctccgtctg cagaaggcgg gcgcgcagta
     7621 tatttttatg caaatgcttc cgtccgcaat tatcacggcg ttcaaaagcg ccgaccgcat
     7681 caattacaat ccggtattcc tgggcacctg gacatcgacg gatcccgact ttttcaaaat
     7741 gggcaaggga ctgattcgcg accggctggt cattcagttt cccggaggat tgccttccga
     7801 caagtccaag ggaatggccg tcatgcaaga actgtggaaa cggtataaaa ccgtcagcaa
     7861 atttgatgcg tcctactggg aaggcgttgt tgtcggcatg attatggaac gtgcctttat
     7921 gcgtgcttat gagaaaagta aaaacattaa tccggcaaca atcaatgcgg cgatggaatc
     7981 attcagaaac gaagatttcg gcggtttggt tccggccatt acctatacaa aagacaatca
     8041 tggcgcttcg tttactgcgc ggatggttaa agtgaaggaa gacggaacgt atattccgct
     8101 gacgaatttc tacgttcccg gcagagataa aatcaagctg ctgggcagta aataaatcat
     8161 gatggcacca ggcgtgatca gaaccttgat tctgatcacg ccgttttcta aagaagaggt
     8221 ttgaatgaaa gcgtttcgca gccggccgtc gattatgatc aaccggcggg aagagaacaa
     8281 gaaagccgaa ctggctattc agaacctgcg cctgagtttc ggcggtgttg ttgccgtcaa
     8341 agatgttgat ctgcaggttc ataccggtga attgatggcg attatcggtc ccaacggagc
     8401 ggggaaaacg agcctgctca actgcatcac cggtttttac cgcgctcagc agggtaaaat
     8461 cgtcttcaac gggaaagaga tcacccgtct gcatacgcac gaacttgtcg gcgtgggcat
     8521 cggcagaact tttcagaata tcgagctttt tccgggcatg accgttctgg ccaatatgac
     8581 actggcgagg catattcatt gccggtacag tttcataaaa tcatttctct tttccaaatc
     8641 agtccgggat gaagaaatcc gtcaccggca ggtccttgaa gagatcatcg attttctgga
     8701 aatgcaatcc atccggaagc aggcggtcgg ttctctgcct tacggcatgc agaagagagt
     8761 tgaactggga cgcgcgctgg ctttggagcc gaagctgctg gtcctggatg agccgtttgc
     8821 cggcatgaac atggaagaga aggaggatat ggtgcgcttc cttctggagc tgaatcagca
     8881 atggggcttg acgatgatcc tggttgaaca tgatatgtcc atcgtcatga gtatttccca
     8941 gcggatcatg gttttgaatt tcggagagaa gctgggtgaa ggaaacgctg aagaaatcag
     9001 cgcaaatcct gaagttataa aggcttatct cggcgagtcc gaagcaatgt aaatatttta
     9061 aggcgtttta attttgagca agggaacatc acatatcacg aaacgcaatc tgacattgcc
     9121 gcaggtgtta atggaaaatg ccgccgtgta cggtgatgca aaaacggcta tccgggaaaa
     9181 ggcatacggt atctggcaga aatacacctg ggcggattac ggtcgctatg ttcggatgac
     9241 ggcggccgga ttggcagcac tgaatctcag gcgcggtgaa aatctgtgca tggtcgtcca
     9301 taatcatccg gaatggctct tttcggaact ggccgcgcat gtcctggggg caacaacgct
     9361 gaatttgttt acatcatcca ttgccgacga actggcgttt tcaatcaaaa ggatcaactc
     9421 acccgttgtg gtggttcagg atcaggaaca ggtggataaa cttctggaga tcaaagaaaa
     9481 acttcctcag acccaaaagg ttgtttatat cgatcccacg ggcatggctt cttatcagga
     9541 cgatccctgg ctgatcagtt acgccggact cttaaaactg ggcgaacaat atcttgaaga
     9601 acatcccggc tttattgact ccgaaatcaa tcggggacgc cccgatgata ttgccgttat
     9661 gatccagaca tcggggacca caggcgttcc caagctggcc atgctgtccc atcgcaacct
     9721 cctgaccatg gccaggcaat ggattgaaga aaccaggatc aaaccggagg aaaactggtt
     9781 gtccatgtcg ccgccggcct ggatcgtcga tcagatgtgg ggcatgggtg tggcgcttga
     9841 cggcgccatg tgcatgaact tcccggaaac ggcggaaacc gttctggaag atttcagaga
     9901 aatcggacct tcgatgatca ttacatcgtc gcggttctgg gaagacatgg cttcgcgcat
     9961 ccgcgtaaaa atttccgatg ccggttggct gaagaagaca ttattttatc tgggcgaaaa
    10021 aatcggcaaa tcggtggtgg aaaaacagtc gcaccgacaa ccggtcggat ggccgttgca
    10081 ggttttgcac agcgtcatgt ccgtgattgt ttacaggccg cttctggatc gtctgggatg
    10141 ttcacatttc cgcagcgcct ttaccggcgg acatccgatc agtcccgatg tcattcattt
    10201 cttccgggcc atcggtctga atttaaaaca atgctacggt ttgacggaat cgtgcggcat
    10261 tttccagatc cagccggatc atgaagtcaa actggaaacc gtcggcaaac ccatggtgct
    10321 gaccgaggtg aaaatcgccg gggatcagga agtgctggtc aaatccgaag cgaatttttc
    10381 aggctatcac aatgattatg aatcaacgca aaacgcgttt gaaaacggct ggctcaaaac
    10441 cggcgatgcc ggatatatcg accatgacgg tcatctgttg attatcggcc gcaaggaaga
    10501 tattatccgc aataaaagcg gcgaggcttt ctcgcctgat tttatcgaga cgcgtcttaa
    10561 attcagtccg tatatcaagg aagctgtgac attcggcgaa gcccgtcctt acattgcggc
    10621 gatgatcaac attgatatgg gcaatgtcgg caactgggcg gaggaaagaa tgattcctta
    10681 tacgacgtac atggatctct cccagcagtc ggcggtggaa gaattgattt tgaaggaagt
    10741 cattgcggtg aatgagcagc ttcccgaggt catgaagatt aaaaagtttg ttctgcttta
    10801 caaactgctg gatgcggatg atgaggaact gacccgcacc ggcaaagttc gccgccgctt
    10861 cgtttacggt ttgtatctga agatcataga ggcgatgtat aacaatttga aaaatgttca
    10921 ggttacggga aaagtgaagt accgcgatgg aaggatcggc gagatagaaa caacgattaa
    10981 tgttattaat gtaaattaag ggacttatta gagatggaat tttttctgca attattggtc
    11041 aacggcgttt gcatcggact gctctacggc atttccgcga tgggctttgt catgattttt
    11101 aaatcgtcaa gtgtgttgaa ttttgcccac ggtgaacttc tggcgctggg agcgtttttc
    11161 tttctgtcgc tggtcacgtg ggcgcagttg ccggtttacg tagcttttat tctgaccatg
    11221 atcggctgtt ttatccttgg ttttctcatc gaaagattct tcctgcggcc gttaatcggc
    11281 gagaaactca ttttcgtggt tatgctgact gtgggactgg cggcaatgtt caaaggtttt
    11341 attctgctcg tatggggcgg aaatttgcac acctatccgg aattcctgcc gccatccatg
    11401 gcgatgcagt tcggttccat ttatgtcgcg cctgcctact cggcaaccct gattatcagc
    11461 gttgtttttc tggtattgtt cggactgttt tttaaaaaat catcccaggg aatttatatg
    11521 cgctccgtgg ccgataacca gaaagccgcg ctttctcttg gcgttcatgt tcgccgggtg
    11581 tttgcgcttt cctgggcgat tgccgcgctg gtggcctgta tgagcggcat tgttctgggc
    11641 atcatcaacg gcgtcaatgt gcatgatctg agctccatcg gactcaaggt tttcccggtt
    11701 gttattctgg gcggcctgga cagcatcggc ggcgccatta tcggcggaat catcatcggc
    11761 cttctggaaa cctttaccgg cggctacctg tcgccgtctt tgcgcgatgt tgttccttat
    11821 atcgtgctgg tgtttatttt aatggtcaag ccttacgggt tgtttggttt ggtggaaatt
    11881 gaaagagtgt aggacgtacg aaaaatgaaa aaattatctt ttcatccctg cggcaattac
    11941 cgggaaacct acgagcagga actgaatatt tttgaaaccg atttcggtcg tttctggatg
    12001 attgtcttcc tgggcgttct tttcttcgtt gcgccgtttg ttcttagccc ctacatgctc
    12061 tacattctca acaccatcgg catcgcggcc attgccgcca tcggcctgaa tattctgatc
    12121 ggttacacgg ggcagatttc tctgggacac ggcgcctttt tcggcgtcgg agcttacgcc
    12181 gcagcgattc tggccacaag actcggcgtc ggattttata ttgcgatccc tgcggcgggc
    12241 atcattaccg cgctggtcgg catgattttc ggcattccgt caggccgtct taaaggtctt
    12301 tatctgacca ttgcaacgct ggccggacaa ttcattatcg aatacgtttt gattcagtgg
    12361 gaaagcctca ccaaaggaac catggggatt acgctgcctt cgcccgaaat attcggatgg
    12421 cagataaccg gcgacagggc gttcttcttc cttgttttta tcagcctgat ctgcatgaca
    12481 tggtttgcgg taaacattat acgcagcaag tacggccggg cgtttatcgc cattcgcgac
    12541 aacgaccgtg ccgcggaagg gatgggcatc ccgattttca aatacaaact gttgtcgttc
    12601 ggcatcagct ctttttacgc cggctttgcc ggagctttat gggcttatta catggtcagt
    12661 attacgacag aacctttcaa tctgggactt tccgttgaat acatcgccat ggtcattatc
    12721 gggggcatgg gtaatattcc cggcgcgatt ttcggcgcga cattcattac actgctcaac
    12781 gaattattac ggttcttaac cggcgtgtta atgaacgtac cggcaattac cagtctgggc
    12841 ttgaatatgg ctccgctaaa agaatttgtt ttcggtctgg cgattgtttt gtttattttg
    12901 attgaaccga aggggctggc ggaaatctgg cgcatcatac gttccagttt ccgtctctgg
    12961 cctttttcat attagaaaat cttgcaattt gctgaaagca aatgcttaga ttttcttatc
    13021 ccgcaaagcg aagcggggct gataaagtga gagaattgag aagctatcca ttagtcattc
    13081 cggtgaaaac cggaatccag gcgtcgtccc ggtgaaggct gggaaccaga tgatggttga
    13141 aaatactgga taccggcctg cgccggtatg acaaaattga tgaattgacc tggttgttaa
    13201 ggggttcaag gtgtttatat ggcagaatgt cttctacaat tgaataacat atcagttgta
    13261 tattcagatg ttatacaggt tttaaaaggc gtttctttaa gtgttgaaaa gggccacatt
    13321 gtctctcttc tgggcagcaa cggcgccggt aagacaacaa cgcttaaagc catttcagga
    13381 ctgcttaaac cggaaaa
//
LOCUS       DAONQZ010000062        13206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5218_length_13206_cov_14.068436, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000062 DAONQZ010000000
VERSION     DAONQZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5218_length_13206_cov_14.068436"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PLR13_09180"
     CDS             complement(<1..1580)
                     /locus_tag="PLR13_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQC19553.1"
                     /translation="MKKVKAESSLKNENKQQFFELDRLILRLSKQFAGSSATEIEKKI
                     GLALQQIVECLDIGRCTFWEVSEDETELRCIRQYVVPKLEKVYGVTPQQSPWISKDQW
                     NGNCSCNVRNEDPVSGAKTNKDASLLLGGKSFIISPFHIAGKAVAGMTFSTLEEDREF
                     SPELVSRLNLIGEIITGAFYRKKIEEKYQQSIGDMRQQSGPARAEDFPLREDTKKEFR
                     YQGIIGNSEAMQNVFIKMEQVAPTDSVVLILGETGTGKGVLARTIHELSNRNKKRIVT
                     VNCAALTPNLIESELFGREKGAYTGSTEMQIGRFELADKGTLVLDEFTELPLDLQAKL
                     LRAIQEGEFERLGSPRTIRVDVRILALTSRNLKEEVSKGRFRQDLYYRLDVFPITMPP
                     LRERLGDIPLLVEHFVEQFNRKMQKEIKNIPGGVMAQLMKYPWPGNVRELEHIIERSV
                     IVTHGTSLQLAEKLEDHSLGLMEENAVTNLAEVEKRHIIKILEKTNWRIDGSKGAAVL
                     LGLHPNTLRGRMQKLGIRL"
     gene            complement(1670..2791)
                     /locus_tag="PLR13_09185"
     CDS             complement(1670..2791)
                     /locus_tag="PLR13_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19554.1"
                     /translation="MTGKNKNRDQAPDQEQKNPVRYALPVKRPLGTILVDGKFIKPET
                     LDAALDQQKQTRDLLGNILVNMGMLDPADLNAALMVQRDFSTIEDAINAAAGVRMMLG
                     ELLLASSRITRDQIEKALEEQKRTGEKIGEILVRLGLFSSTEMDIALSFQSRQNAGYA
                     SCLSLGELLIAAGYITHDDLEDALERQKGNNRKLGEILVDAGRCKPHHVEHGLRMQKQ
                     LVAAALIAALSLAPVKEAVSSVNPSSTAMTTNIKVTATVLARASMHVLRQPNEIVVTD
                     ADIKRGYLDISAGSLVEIKNNSRAGVDLTFETHGLPFSEVLVSGFGREVALGPNGGII
                     THQLLGANIIAFSYRFVFDRNSRAGTYAWPLVLSVSPVE"
     assembly_gap    2902..3001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3044..4525)
                     /locus_tag="PLR13_09190"
     CDS             complement(3044..4525)
                     /locus_tag="PLR13_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19555.1"
                     /translation="MKKKHNTIIRGAGIFLIILFCSWLPSAVLAAEDPFASFLQSNVK
                     QYTATDAEYADRPYLRKLQGAFNADFRILTYGVLQDPAKSTQNPNNDPVGFPNYIGNI
                     DVRPDFRFNTKYLDLVFKPRAMFDYRYWKEGQREGEDDWNDEWYVNEWLVRLKAAERV
                     FLSYGRENLQWGPSFLYSPSNPFFFDNGRSNPQMEVAGMDFARVVFVPHMLWTVSFMV
                     NTDQGRNTLISTDPFEETYAAKIDYTGNQNYASLILSHKDDDTTLGYFGGWTVSDALL
                     LYSEGSMRQGSDALYPNLKRRSLGQSFLEALFAPNSELRPILDRYTSLRSAYMDKTRE
                     DDSKIYPTILVGGAYTFEDIGTLTLEYMYHAQGYDEDEADLYYSLRGRAAYAYLDMGA
                     YPGLLGQFTLYQTGNTGLRFLRKNYTMLQYYKGNIFNRMETTLRWTQNVDDGSAQFLG
                     LLSYSLGNHVELFSSGVLNTGKEDSEFGTFINYQVMFGLKYTL"
     gene            complement(4535..5308)
                     /locus_tag="PLR13_09195"
     CDS             complement(4535..5308)
                     /locus_tag="PLR13_09195"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HQC19556.1"
                     /translation="MMSRFLKKNYFTLWSFVVLFFLNVAPLQAMTNQEMLEQADKARG
                     NEEGIQWEIKMHSVEKGRVQDRTLLVIAKGNSASGYNSLIDTLEPGNLKGQKLLMQDR
                     NMWFAKPGLSKPVPISPRQKLMGDASNGDIAATNYAGDYKIIGSEDGQFNNEDCVVLN
                     LQAVDKRATYDRIKYWISKKRVLGVKAEFYTISGKMFKTAIFEYKNRITVEGKSQEFI
                     SQMTITNAIMKDDITVVKYSRPVLKKVSDATFNLNLLTK"
     gene            complement(5330..6568)
                     /locus_tag="PLR13_09200"
     CDS             complement(5330..6568)
                     /locus_tag="PLR13_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQC19557.1"
                     /translation="MMNWMKLAFRNILRNKRRSFVTMVAIGVGFAAIGLYHGYIHNAY
                     AGLRVIAIRGEGLGHLRINKAGWNAKGKLEPEKYMFTREETEKIMKLVGEEKDVVLST
                     PQMQVVGTVTNGATSTVFIAQGVVPKDEVIIKGDWVVFKPRAGHKISDDVLYGVEMAK
                     DLAAYLNMNAGSNGIVMAQTLAGQVNALDMQVSGVYDTGNDFSNDKFMRFNFKFAQSL
                     LDTQSAERVVVLLKEWEDTEMMRDRLLKKLKAAGIDCEIRTWKELSLGYLKMKSYLDM
                     IFIFIASIVLGIVIMTTINTMGMAILERTKEIGTLRALGLKVKGVSLLFTLEGALLGF
                     LGSIVGILLHIVVWVIIRLFPPHYVPPGFSEPVPMFIDMVPWMLLLLVFCFIILSMIA
                     AIIPARGAARKNIVDALGHV"
     gene            complement(6628..7890)
                     /locus_tag="PLR13_09205"
     CDS             complement(6628..7890)
                     /locus_tag="PLR13_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQC19558.1"
                     /translation="MLQWIKLAYRNILRNKRRSVVTMIAIAVGFTSISIYHGYINNAY
                     EGLRWLAICGERLGHLRINKAGWEEQGKLEPHNYMFSKEQTDKIIQLVKEEKDVVLVT
                     PQIQVMGTVTNGLTSIIFIAQGVIPKDEEFITQFWFDVVGYVDEGYDAVIGEKLNESK
                     TYGVLMAKDMASYLKMKPGSDGIVMAQTLSGQMNAMDMQISGVYDTGNDFSNDKFMRF
                     NFNFAQALLDTQSAERVVVMLKHWKDTEQMHDVIAKKLKNAGIDAEIRTWQDLSIAYN
                     KMKSYLDTIFIFLFFIVVTIVLVTIINTMSNTIVERTKEIGTLRALGMKRVGVAKLFA
                     LEGALLGVFGSMAGVVLHTIEWSLIRLFPLHYTPPGLSTPVAMRVNMAPWALMLLIVC
                     FIIISTIAAVIPAKSAAKANIVDSLGHP"
     gene            complement(7900..8574)
                     /locus_tag="PLR13_09210"
     CDS             complement(7900..8574)
                     /locus_tag="PLR13_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19559.1"
                     /translation="MALIEMKSITKDYHLGETVVHALRGIDLQIDKKEFVAVWGPSGS
                     GKTTLLNLIGAIDDPTSGELAIAGKNVQKLTDDQKSEHRNETIGFVFQSFNLVPVLSA
                     LENVMLPMQIRGASVAEAKKQALMRLEEVGLKDLIHNRPAKMSGGQQQRVSIARALVN
                     NPSLVIADEPTANLDSETAHMVIDIMRELNERDHITFIFSTHDQRLLDKVKRLVRLED
                     GKIIQE"
     gene            complement(8807..9715)
                     /locus_tag="PLR13_09215"
     CDS             complement(8807..9715)
                     /locus_tag="PLR13_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQC19560.1"
                     /translation="MASKFSKNYFVVILCAVFLLFSFNDVFAKSSKKSSHHRKHSTSQ
                     SVQKKKGGLVLRSAAVIVKDQQTGKLLIQKQAKAVMPIASITKLMTAMVVLDAGLDPD
                     ETITIEEEDATAVRGTSFRLAVGTDLTREEALLLALMSSDNRCAHALGRTYPGGYWNC
                     VRAMNAKAAALGLAHTRFRDTVGLSSNNVSSAKDLARLVDTAYQYEMIRDFTTRKKAT
                     IFTGWQMLKFRNTNQLVRDGCGWKIGLSKTGFTNEAGRCLVMQAKVAKRPLLIVLLDC
                     RNNKARINDANRIKSWLESKSHSQHT"
     gene            complement(9894..10733)
                     /locus_tag="PLR13_09220"
     CDS             complement(9894..10733)
                     /locus_tag="PLR13_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQC19561.1"
                     /translation="MRVPENWASVIRLASFTIADEARQKSFIAILIICALFILMVRGC
                     YHGDYMMNGQVVDSAKIIIMVSKITFHIIAGGVLFLAALLSMRIFRRDRGDGTQSCIL
                     SKPVSRRQYVSGKILGLWVLLTAVMIVLHGIVFLITSVSANMVMPEFFSASIWCSLNV
                     LFVVLTVLLLSMMMPDIAALLCVIGIGMIGVAADGVFAVRHSPIGQAMMQHDAPSDFS
                     VWQIIYTVWPKLFGVQSFAASRIGAEGFGGPVFAVPILNVGIYCMIIGALLFWRFGKE
                     DID"
     gene            complement(10735..11442)
                     /locus_tag="PLR13_09225"
     CDS             complement(10735..11442)
                     /locus_tag="PLR13_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19562.1"
                     /translation="MIELDAVTKKYGSLVAVDNVSFSVGAGECFALLGPNGAGKTTIV
                     KLLLDFTRPTAGHLSLNGLSTTNAKSRAFVGYLPENHRIPTHLSGWQYLKRCAALLNL
                     KGTDADKQCQWAVTLTGMQGREHDKSGTYSKGMIQRFGLGAALIGRPKLLILDEPAAG
                     LDPIGIRDIRRLLESLKKDGMTVFLNSHLLSEVEKICDNAAVIRRGRLMVRDKISSLV
                     RDGDTLEDVFIRFVKEG"
     gene            11590..13125
                     /locus_tag="PLR13_09230"
     CDS             11590..13125
                     /locus_tag="PLR13_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQC19563.1"
                     /translation="MKTFPTLIRILLFFFLTASSCFGAEQVQFRILHLNDFHGFAEPQ
                     RLSGSAQTAGGLSFLAGSINQFRKHQPTLLLAAGDFIQGHPWTNLFEGKSTIEAMNVM
                     GFSAMVLGNHEFDFGQDVLKKRIAEAAFPVLAANVRGFPSVKPYVIKDISGLKVGIIG
                     LITEETSTTTHPKNVEGLIFSPVIDTAQQLVRELRNKTDLIIILSHLGLPADVALAGA
                     VEGIDIIVGGHSHTRVEKPMIVRNTLIVQAWEHGKVLGVLDVTVRNKKISGHEGRLIV
                     IDPVQNKPDPPVAQIVDRYQTQAAGILNHVIGEARVDLPGKESRCRETSLGNLVADIL
                     RQQTGADIALINGGGIRADIWKGPVRMNDILSVLPFSNRAIVLKVSGQEIKDIFEHGI
                     SDPEGKAGRYPQVSGMQLTYNPANPAGQRITGFTINGKPLQTDQRYTLATSDFLAAGG
                     DGYAAFTRILKTMNDGGQNTASENRALFFASGQDIRDMVIDFIRDNKMISAAVENRIR
                     KED"
BASE COUNT         3298 a         3409 c         3025 g         3374 t
ORIGIN      
        1 agccggatgc ccagtttctg cattcgtccg cgcagcgtat tgggatgcaa accaagcaga
       61 acggcggcgc ctttggaacc gtcgatccgc cagttggtct tttccaggat tttaatgata
      121 tgccgttttt caacttcggc aagattcgtg actgcatttt cttccatgag acccagggag
      181 tgatcctcca gcttttccgc aagctgaagg ctggtgccgt gcgtaacaat aacggaacgc
      241 tcaatgatat gctccagttc ccggacattg cccggccacg gatacttcat cagttgcgcc
      301 ataacccctc cgggaatgtt tttaatttct ttctgcattt tccggttaaa ctgttccaca
      361 aaatgctcca ccagcaacgg aatatcgccg agtctttccc ggagaggcgg catggtgatg
      421 ggaaaaacgt ccaaacgata gtaaaggtcc tggcgaaacc gtcctttgga aacttcttcc
      481 ttcagattcc tgctggtcag agcaagaatg cgcacatcaa cccggatggt ccggggagaa
      541 ccaagccgtt cgaattctcc ttcctggatg gcccggagaa gtttggcctg caaatcaaga
      601 ggaagttccg taaactcatc gagaaccagc gttcccttgt cggccagctc aaagcggccg
      661 atctgcattt cggtggatcc ggtataggcg cccttttccc ggccgaatag ttcgctttcg
      721 atcagattgg gcgtgagagc ggcacaatta acggttacga ttcttttttt gtttctgttg
      781 ctgagttcat ggatggtacg ggccaaaacg ccttttcccg ttcccgtttc gccgaggatg
      841 agaacaacgg aatcggtcgg cgcaacctgc tccattttga taaaaacatt ttgcattgct
      901 tcggaattac cgatgatgcc ctgatatctg aattcttttt tcgtgtcttc ccgcagcggg
      961 aaatcctctg cccgggccgg accgctctgc tgacgcatgt cgccaatcga ctgctgatac
     1021 ttttcctcga tttttttccg gtaaaaagcg ccggtgatga tttccccgat caaattcagc
     1081 cgggaaacaa gctccggtga aaattccctg tcctcttcca gggtggaaaa cgtcatgccg
     1141 gcaacggctt tgccggcgat atgaaagggg ctgataatga aagacttgcc ccccaacagc
     1201 aacgaggcgt ctttgttggt tttcgctcct gagactggat cttcgttacg gacattgcac
     1261 gaacaattgc cgttccattg atctttggaa atccatgggg attgctgagg ggtaacgccg
     1321 tacaccttct ccagttttgg aacaacatac tggcggatac agcgcaattc ggtttcatct
     1381 tccgatactt cccaaaatgt gcagcggccg atatccagac attcgacaat ttgctgcaat
     1441 gccagcccta tttttttctc gatttcagtt gcggaagatc cggcaaactg cttggaaagt
     1501 ctcagaatca gtctgtcgag ttcgaagaac tgttgcttat tttcattctt taaggaagat
     1561 tccgctttca cttttttcat tttatgattg caaaaaggct gtcatgttaa aatcggcttt
     1621 ttccccgtct gaatgatacg tccaagccgc tgcaattcaa ccctatcttt tattcgacag
     1681 gactcaccga gaggaccaga ggccaggcat acgttccggc ccgcgagttt ctgtcgaaaa
     1741 caaaacgata gctgaacgca atgatgttgg cgccgaggag ctggtgggtt atgattcccc
     1801 cgttgggtcc cagtgccact tcccggccaa agccgctgac cagaacttct gaaaacggca
     1861 ggccatgagt ttcaaaagtc agatcgacgc ccgctcggga attgtttttt atttcgacca
     1921 gagagccggc gctgatgtcg aggtatcccc gtttaatatc ggcatccgtc accacaattt
     1981 cgttcggttg acgaagaaca tgcatgctcg cacgcgccag cacggttgca gtcactttga
     2041 tattggtggt catggccgtg gaagacggat ttaccgagga tacggcttcc ttgaccggcg
     2101 ccagggataa agcggcaatg agcgcagcag caaccagttg cttttgcatt cgcagaccgt
     2161 gctcgacgtg atgcggttta catcggccgg catcaacaag aatttcaccc agtttcctgt
     2221 tgtttccctt ttgacgctcc agagcgtctt caagatcatc gtgcgtgatg tatcctgcag
     2281 cgatcaggag ttcgcccagg gacaggcagg acgcataccc cgcgttctga cgggattgaa
     2341 acgacagcgc gatatccatc tccgttgacg agaaaagccc gagccttaca agaatttctc
     2401 ctattttttc gcctgttctt ttttgctctt caagcgcttt ctcgatctga tcgcgggtaa
     2461 tgcgcgatga ggccagaagc agttctccca gcatcatccg gacgcctgcg gcagcgttta
     2521 tcgcatcttc aatggtcgaa aaatcacgct gaaccatcag agcggcattc aggtcggcag
     2581 gatccagcat gcccatgttc acgagaatgt ttcccagaag gtcccgggtt tgtttctgtt
     2641 gatcaagagc ggcatccagg gtttccggct tgatgaattt gccgtccacc agaatggtcc
     2701 cgagcggcct tttcacggga agagcatacc ggacaggatt tttttgttcc tggtccggag
     2761 cctgatcccg gtttttattt tttccagtca tcttgtatcc tttgggcggg ttaaagatca
     2821 gaatagctta ccacggacaa tcagtgaaag gttgaacgca tcgtctctat ttcacactat
     2881 tagaatactc acaattgctg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 natgaaacga cggagctatt ccatgctccg tcgtatattg acgctagagc gtatatttca
     3061 acccgaacat aacctgataa ttgatgaagg tgccgaattc cgaatcctct tttccggtat
     3121 tcagcacacc ggatgaaaac aattcgacat gattgccgag ggaataggat aaaagtccca
     3181 gaaactgagc cgacccgtca tccacattct gtgtccagcg cagcgtagtt tccattctat
     3241 tgaaaatatt tcccttgtaa tactgaagca ttgtgtaatt tttacgcaaa aatcttaagc
     3301 ccgtattgcc ggtctgatac agcgtaaact ggcccagcag tcccggatag gctcccatgt
     3361 caagatacgc ataggcggcc cggcctctga gcgagtaata gagatccgct tcatcttcat
     3421 cataaccttg cgcatgatac atgtattcca gggtgagggt accgatatct tcaaacgtat
     3481 aggctccgcc gaccagaatt gtcggataaa tcttagagtc atcctcccgt gttttatcca
     3541 tatacgccga ccgaagcgag gtgtatctgt ccagaatcgg cctcaattcg gaattcggcg
     3601 caaacaaagc ttccaggaac gactgaccga gtgatcttct cttaagattc ggatacaaag
     3661 catcgcttcc ctgacgcatg ctgccttcac tgtaaagcaa cagggcgtcg gaaaccgtcc
     3721 agccgccgaa ataccccaag gtcgtatcat catccttgtg agataaaatc aaagaagcgt
     3781 aattctggtt tcccgtgtaa tctattttag cggcatatgt ttcctcaaaa ggatcggtgc
     3841 tgataagagt attacgtccc tgatccgtat tgaccatgaa agagaccgtc cagagcatgt
     3901 ggggaacaaa cacgacacgg gcaaaatcca ttccggcaac ttccatctgg gggttgctgc
     3961 ggccgttatc aaagaaaaac ggattcgagg gagaataaag gaaagaaggt ccccattgca
     4021 ggttttcccg tccgtaggac aagaagactc tctctgcggc cttcaatctg accagccatt
     4081 cattgacata ccattcatcg ttccagtcat cctcaccttc acgctggcct tctttccagt
     4141 agcgatagtc aaacatcgcc cgcggcttga aaaccagatc aaggtatttg gtattgaaac
     4201 ggaaatccgg tctgacatca atattcccga tgtaattggg aaatccaacc ggatcgttgt
     4261 tcgggttttg cgttgatttg gcgggatcct gaagaacccc gtacgttaaa atccggaaat
     4321 ccgcattaaa agcgccctgt aatttcctca ggtaaggccg gtccgcatac tcggcatcgg
     4381 tcgccgtgta ctgtttgacg tttgactgca gaaaagaggc gaaaggatct tcggcggcca
     4441 gcaccgctga aggcaaccat gaacaaaaga ggatgatgag aaaaataccc gctcccctga
     4501 ttatggtatt gtgctttttt ttcataaata acctctattt tgtcagtaaa tttaaattaa
     4561 atgttgcatc ggaaactttt ttcagcaccg gccggctgta tttcacgacc gtgatatcat
     4621 ccttcatgat cgcattggtg atggtcatct gggaaatgaa ttcctgagac ttaccctcca
     4681 ccgtaattct gtttttatac tcgaatatcg ccgtcttaaa catctttccg gaaatcgtgt
     4741 agaattcagc tttcacgccc aaaacccttt ttttggatat ccagtacttg atccggtcat
     4801 acgtggctct cttgtccact gcctgtaaat tcaaaacgac gcaatcttcg ttgttaaact
     4861 gcccgtcttc cgatccgata attttataat cacccgcata gtttgtggcc gcaatatcgc
     4921 cgttggacgc atcgcccatc agtttctgcc gcggtgaaat aggaacgggt ttggacagac
     4981 ccggttttgc aaaccacata ttgcgatcct gcatcagcag cttttgcccc ttcagattgc
     5041 cgggttccag cgtatcaatg agggaattat aaccgctggc gctgtttcct ttggcaataa
     5101 ccagcaaggt tctatcctgc acacgtcctt tttccacgga atgcatttta atttcccact
     5161 gaatgccttc ctcgttgccc cgcgccttgt cggcctgttc cagcatttcc tgattggtca
     5221 tcgcctgcaa cggcgcaaca ttgagaaaga ataatacaac aaaactccat agggtaaaat
     5281 agtttttctt taaaaatcga ctcatcattt tctccctgat ctataatttt tatacatgtc
     5341 ccagcgcgtc cacaatattc ttcctggccg cacctcttgc aggaatgatc gctgcaatca
     5401 tggacaatat tatgaaacaa aataccaaca gcagcaacat ccacggcacc atatcaataa
     5461 acatgggcac cggctccgaa aagccgggag gaacataatg aggcggaaac agccggataa
     5521 tgacccacac aacaatgtgc agcaaaatac ccacgatact gcccagaaat ccgagcaacg
     5581 ctccctccag agtgaaaagc aaggaaacac ccttgacttt gagtcccagc gcccgcaggg
     5641 ttccgatctc tttggtcctc tccagaatgg ccatccccat ggtattgatg gttgtcatta
     5701 tcactatccc cagcacaatg gaagcaataa aaataaatat catgtccaga taggacttca
     5761 tttttaaata acccaaagag agttctttcc aggttctgat ttcacaatca atgccggcgg
     5821 ccttcagctt cttgagcaga cgatcgcgca tcatttcagt gtcttcccat tctttcagca
     5881 gaaccaccac gcgttccgct gattgcgtat ccagcagaga ctgcgcaaat ttaaaattga
     5941 agcgcataaa tttgtcattg gaaaaatcat tgccggtatc gtagacaccg ctgacctgca
     6001 tatccaaagc gttcacctga ccggccagcg tctgtgccat gacaatcccg ttgctgcccg
     6061 cattcatgtt cagataggca gccaggtcct tggccatttc aactccgtag agtacgtcgt
     6121 cgcttatttt gtgccccgcg cgcggcttaa aaaccaccca atcccctttg atgatgactt
     6181 cgtctttggg cacaaccccc tgagcgatga aaaccgtaga cgttgccccg tttgtcaccg
     6241 ttccgacgac ctgcatttgc ggtgtggaca agacgacatc tttttcctct ccgacaagtt
     6301 tcatgatctt ttcggtttct tcccgggtaa acatgtattt ttcaggttcc agcttgccct
     6361 tggcgttcca cccggccttg ttaattctca aatggccaag tccttcgccg cgaatcgcaa
     6421 taacccgcag tccggcataa gcattgtgaa tgtagccgtg gtaaaggccg atcgcggcaa
     6481 accctactcc gatggccacc atggtcacaa aggatcgccg cttgtttctt aaaatattcc
     6541 ggaatgccag tttcatccag ttcatcatag gtttaatcca gccgcctttc agaaatgttc
     6601 agtttatctt tttaatccaa aaatatttca gggatgtccc agtgaatcta caatattcgc
     6661 ttttgccgct gattttgcgg gaatgacggc tgcaatggtc gatatgatga tgaaacagac
     6721 tatcaagagc atcaacgccc agggcgccat attgactctc atggccacgg gcgttgacaa
     6781 tcccggaggt gtatagtgca gcgggaagag cctgatcagc gaccattcaa tcgtatgcaa
     6841 gacaacgcct gccatactgc cgaaaacgcc cagcaaagcg ccttccagag caaaaagctt
     6901 agccacgcca acgcgtttca tccccaaagc ccgcagggtt ccgatttctt tcgttctttc
     6961 aacaatggta ttgctcatgg tattgataat cgtcaccagc acgatcgtca ccacgatgaa
     7021 aaaaagaaaa ataaagatcg tatccagata cgatttcatt ttattataag cgatggaaag
     7081 atcctgccag gttctgattt cagcatcaat accggcgttt ttaagttttt ttgcgatgac
     7141 atcatgcatt tgttccgtat ctttccagtg tttgagcatg accacaaccc tctccgccga
     7201 ttgcgtatcg agcagggcct gggcgaaatt aaaattaaac cgcataaatt tgtcattgga
     7261 gaaatcgttt ccggtatcat agacgccgct gatttgcata tccatggcat tcatctgacc
     7321 gctcaaggtt tgcgccatga cgattccgtc gctgcccggt ttcatcttta gataactggc
     7381 catgtctttg gccatcagca caccgtaagt tttactttcg ttcagttttt caccgatgac
     7441 cgcatcatac ccctcgtcca cataaccgac gacatcaaac cagaactgtg taataaattc
     7501 ctcatctttc gggataacgc cctgggcaat gaaaataatg gaggtcagac cgttggttac
     7561 cgtgcccatc acctgaatct gcggtgtaac cagcacgaca tctttttctt ccttgaccag
     7621 ttgaataatt ttatccgtct gctctttcga aaacatatag ttgtgcggtt ctagcttgcc
     7681 ctgttcctcc catcccgctt tattgattct taaatggccc aaccgttcgc cgcatatcgc
     7741 aagccaccgg agtccctcat aagcgttatt aatatagccg tgataaatac tgatggaggt
     7801 aaaccccacg gcaatggcaa tcatcgtcac aacagagcgg cgtttgtttc ttaaaatatt
     7861 ccggtaggcc agcttaatcc actgcagcat cagacgttat cactcctgaa taattttgcc
     7921 gtcttccaga cgtaccagcc gtttgacttt atccagaagc ctctggtcgt gtgtggagaa
     7981 aatgaacgtg atgtggtctc tttcattcag ttcacgcata atatcgatta ccatatgggc
     8041 cgtttccgaa tcgagattgg ccgtcggttc atcggcaata accagtgagg gattattgac
     8101 cagcgcccgg gcaattgaaa ctctctgctg ctgaccgccc gacattttcg ccggacgatt
     8161 atgaatgaga tccttcaggc cgacctcttc cagacgcatc agcgcctgtt ttttggcttc
     8221 cgccaccgac gcgcctctga tctgcatggg cagcatgaca ttttccagtg cggagagaac
     8281 aggcaccaga ttaaagctct gaaaaacaaa accgatggtc tcattccgat gctcgctttt
     8341 ctgatcgtcc gtcaacttct ggacattttt ccctgcaatg gccaattctc cggatgtggg
     8401 atcatcaatg gcaccgataa gattcaggag ggttgttttg cccgatccgg acggtcccca
     8461 aacggccaca aattctttct tgtcgatctg gagatcaatg cctctcagcg catggacaac
     8521 cgtctcgcca agatgatagt ccttggtgat actcttcatt tcaattaaag ccattttata
     8581 ccccctggaa aaactcaacg gtaaaccgtc atggattgcg gccgacactt tgcaaagcaa
     8641 tcattttttt ggcttgcgga gcatataaag aaatgtccta tatgtcaatt aaatatcaaa
     8701 aaaaaataga taaaaaaata aaggcggtgg ttaggatgtt attaattttc aattgaaaac
     8761 cgcttcacac cggctctcaa tttcaaccga tacgatatac ccattattat gtatgttgag
     8821 aatgtgattt tgattccaac caggatttta tccggttggc atcgttaatg cgtgccttat
     8881 tgtttcggca atcaagcaga acgatcagca gcggacgttt cgccactttg gcctgcatga
     8941 ccagacacct tccggcttca ttggtaaatc ctgttttgga cagaccgatt ttccagccgc
     9001 aaccgtcccg gacaagctgg ttggtgttgc ggaatttcag catttgccag ccggtaaaga
     9061 tcgtggcctt tttacgggtc gtaaaatcac ggatcatctc atactgatag gcggtatcaa
     9121 ccagtcgcgc caggtctttt gcggaagaaa cgttattgct cgacaggcca accgtatctc
     9181 tgaaacgcgt atgggctaat cccaacgcag cggcttttgc gttcatcgcc cggacacagt
     9241 tccagtaacc gcccggatac gttctcccca gtgcatgggc gcaacggtta tccgaagaca
     9301 tcagcgccag caacaacgct tcctcgcggg tgaggtcggt gcccacggca agccgaaagc
     9361 ttgtcccgcg aaccgctgtc gcgtcttcct cctcaatggt gatcgtttca tcaggatcca
     9421 gtccggcgtc aagaaccacc atggcggtca tcagtttggt gatggatgca atgggcatga
     9481 cggctttggc ctgtttctga atcaacaact taccggtttg ctgatccttc acaatcactg
     9541 ccgccgaacg caaaaccagc ccgccctttt tcttctggac ggattgtgat gtggaatgtt
     9601 ttcgatgatg ggacgatttt ttggaggatt ttgcaaatac gtcgttaaag gaaaataata
     9661 aaaaaactgc acataaaatg actacaaaat aatttttact gaatttactt gccatgcatt
     9721 ctcccgatct tcaagatacg tctttagttt tgcaacaaag acctgagtgt caatttaatg
     9781 ccggcatttg ctctgccaac ttgtgtttca aagttaattt tttggaatca tacagaattt
     9841 tttcgaatta gtcaattttg aatttgattt ttttgcaaaa aattccaggg atgtcaatca
     9901 atgtcttctt ttccgaaacg ccagaacagc agtgcgccga taatcatgca gtagatgccg
     9961 acattcagga tcgggacggc aaaaaccggt ccgccgaatc cttcggcgcc gatccgggaa
    10021 gcggcaaagg actgcacgcc gaaaagtttc ggccacaccg tataaattat ttgccaaacg
    10081 gaaaagtcag atggcgcatc gtgctgcatc atcgcctgtc cgatcggact atggcgaacc
    10141 gcaaagacgc cgtcggctgc aacaccgatc atcccgatgc cgataacgca aagcaacgcg
    10201 gcaatatcag gcatcatcat cgacaaaaga agcacggtca gcaccacgaa aagcacattc
    10261 agcgagcacc agatcgacgc cgaaaaaaat tccggcatga ccatgttggc gctgacggat
    10321 gtgatcaaaa aaacgatgcc atgcagaaca atcatgacgg ccgtcagcaa aacccacagg
    10381 ccgagaattt ttccggagac atactgccgg cggctgacgg gttttgaaag gatgcaggat
    10441 tgcgtgccgt caccgcggtc gcggcggaaa atgcgcatgg acagcagagc cgccagaaac
    10501 aaaacgcccc cggcaatgat atggaatgtg attttcgaaa ccatgatgat gatttttgct
    10561 gaatcgacga cctgtccgtt catcatgtaa tcgccgtgat aacaaccgcg caccatcaag
    10621 ataaacaaag cgcagatgat cagtatcgcg ataaaacttt tttgacgggc ttcgtccgca
    10681 atggtgaatg aggccaggcg gatgacggaa gcccagtttt ccggaactct catgtcaacc
    10741 ttccttcaca aaacggataa aaacatcttc cagggtatcg ccgtcccgga cgagagaaga
    10801 tattttatcc ctgaccatca gccttccgcg acggatcacc gcggcgttat cgcatatttt
    10861 ttccacttcc gacaaaagat gggaattcag aaaaaccgtc ataccgtcct ttttcagcga
    10921 ttccagtaac cggcgtatgt cccggatacc gatgggatca agtcctgcgg caggttcatc
    10981 caggatcagc agtttcggcc ggccgatcag agcggcgccc agaccaaatc gctgaatcat
    11041 acccttggaa taggttccgg atttgtcatg ttcccttccc tgcatgccgg tcagtgtgac
    11101 cgcccactgg cactgcttat ccgcatcggt acctttgaga ttgagcagtg ccgcgcagcg
    11161 cttcagatac tgccacccgg aaagatgcgt cgggatacgg tgattttccg gcagatagcc
    11221 gacaaaagcg cggctcttcg cgtttgttgt ggaaagaccg ttcaacgaca aatgtcctgc
    11281 agtcgggcgg gtaaaatcaa gcagcagttt aacgattgtc gttttccccg ctccgttcgg
    11341 tccgagaaga gcaaaacact cgcctgcgcc gacggaaaat gaaacattat ccacggcaac
    11401 cagggagcca tacttttttg tcacggcgtc gagttcgatc atcacgaatg aacatatggt
    11461 ccgaaggaca ttctgtcaac aaaattatca taaaaacgcc tgccgctctt tccgtcccgt
    11521 aaacagctct ggaattcttc cggccattgg gctatattaa aaaaagtttg gaaaagaagg
    11581 aatttttcca tgaagacatt tccgacattg atccgcattc ttttgttttt ctttctgacc
    11641 gcttcatcct gtttcggtgc ggaacaggtt cagtttcgga ttctgcatct gaatgacttt
    11701 catggttttg ccgagccgca acgcctgtcc ggatcggcgc agaccgccgg cggattatct
    11761 tttcttgccg gatcaatcaa tcagttcaga aaacatcagc cgacactgct tctggctgcc
    11821 ggagacttta ttcaggggca tccgtggaca aacctttttg aaggcaaatc caccatcgag
    11881 gcgatgaatg tcatggggtt ttcggcaatg gtcctcggaa atcacgaatt cgatttcgga
    11941 caggatgttc tcaaaaaaag gattgccgaa gcagcttttc cggttctggc cgccaatgtc
    12001 cgcggtttcc cgtccgtcaa accgtatgtg atcaaagaca tctccggcct gaaagtgggg
    12061 atcatcggat tgatcacgga agaaacatcc accacaacgc atccgaaaaa tgttgaaggc
    12121 ctgatttttt ctccggtcat tgataccgcg caacaacttg tccgcgagtt gagaaataag
    12181 accgatttga tcatcattct ttcccatctt ggcctgccgg ccgatgtggc cctggccggg
    12241 gccgttgaag gaattgacat cattgtcggc ggacattcgc atacccgcgt tgaaaaaccg
    12301 atgatcgttc gtaacacact gattgttcag gcgtgggaac acggcaaagt gctgggggtt
    12361 ctggatgtaa cggtccgcaa taaaaaaatc agcggacatg aaggacggct gatcgtcatc
    12421 gatccggtcc aaaacaagcc ggatccgccg gtggcacaga ttgttgatcg ctatcagaca
    12481 caggcggccg gcattctcaa ccatgtgatc ggcgaagccc gggtcgatct gccggggaag
    12541 gagtcccgct gccgggaaac ttctctgggc aatctggtcg ccgatatttt aagacagcaa
    12601 accggcgccg atatcgcttt gatcaacggc ggcggcatcc gggcggatat ctggaagggt
    12661 ccggttcgta tgaacgatat tctttccgtc ctgcctttct ccaatcgcgc tattgtcctg
    12721 aaggtcagcg gacaagaaat caaagatatc ttcgaacacg gcatttccga tccggaaggg
    12781 aaggcgggac gttatcctca ggtttccgga atgcaactga cctacaatcc cgccaacccc
    12841 gccggtcaga gaattaccgg ctttacgatc aacgggaaac cgttgcagac cgaccaacgg
    12901 tacacactgg cgaccagtga ttttctggcc gccggaggcg acggctatgc cgcgttcacc
    12961 cgaattctta agaccatgaa tgacggggga caaaacaccg catcggaaaa tcgcgcgctg
    13021 ttttttgcat ccggacagga cattcgcgac atggtcattg attttatcag agacaataaa
    13081 atgatttccg ccgccgtgga aaacaggatc agaaaggagg attgattgat gcgtgtctgg
    13141 ttaaataatg aaccggtcga tgtggttccc ggaatgaaag ttcgcgaagc cctgattcaa
    13201 gcaggc
//
LOCUS       DAONQZ010000063        13041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5305_length_13041_cov_18.163638, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000063 DAONQZ010000000
VERSION     DAONQZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_13041_cov_18.163638"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1217)
                     /locus_tag="PLR13_09235"
     CDS             complement(216..1217)
                     /locus_tag="PLR13_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQC19564.1"
                     /translation="MLDSMAFVVFLIIGALAFDFLNGFHDSANSISTVVSTRVLSPKY
                     AVIWAAFFNFAAVFFVGTAVAHTVGKGIINIDIVDNLVILAALGGAIIWNIITWYYGL
                     PSSSSHALIGGLIGAAISKAGFSTLVWAGIIKTTVFIIVSPAIGMALGFIFMVTAMNL
                     SHNSNIGKSDKLFRKLQLLSAAVYSLGHGMNDAQKTMGIIAMALFSKGLLGDTFYIPV
                     WVIIACYSMISLGTMFGGWRIVKTMGTKITKLQPMGGFSAETAAACSIIGATVAGIPV
                     STTHTITGAIVGVGSTRRLSAVRWGVAGKIIWAWILTIPAAAILSSIIYLCVQLFIT"
     gene            complement(1210..1830)
                     /locus_tag="PLR13_09240"
     CDS             complement(1210..1830)
                     /locus_tag="PLR13_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQC19565.1"
                     /translation="MRLFPKEENFFELFDELADKIEEGGKLFLEMAQTRDYSDVRVAK
                     LKEIEHEADGITHKTYEKMHKTFLTPIDREDIYALVNKMDSIMDVIEATAVRIHLYKV
                     KRTSDEIIKQAQILNDAIKKLKTIIQAMRNMKNSDMILAGCVEINTLENAGDITLRSI
                     MANLFETEKDAIELVKWKDIFQLLEEAIDVCEDVSNIVEGIVLKHA"
     gene            complement(1969..2454)
                     /locus_tag="PLR13_09245"
     CDS             complement(1969..2454)
                     /locus_tag="PLR13_09245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19566.1"
                     /translation="MKKFIYRQQIKPSDIEVIADMVESSGFFSDEEIEIAIELAEDKL
                     DKQEVSSYQFLFAEIENQVVGYTCYGLIPATAGSYDLYWIVVAKDLQGLGVGKMLMAE
                     TENRIYQNGGRQIYAETSSRAQYTPTHKFYESCGYAKEAFLKNFYSEGDGKIIYAKVL
                     K"
     gene            complement(2463..2810)
                     /locus_tag="PLR13_09250"
     CDS             complement(2463..2810)
                     /locus_tag="PLR13_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQC19567.1"
                     /translation="MLKTIIFLFFSNLFMTFAWYGHLKNFNHKPLLFVILFSWGVAFF
                     EYVLQVPANRIGFHYYSLGQLKVIQEVITMIVFAGFAIMYMKQPLKMDFLWAGLCLVG
                     AVYFMFREGLPHT"
     gene            complement(2816..3817)
                     /locus_tag="PLR13_09255"
     CDS             complement(2816..3817)
                     /locus_tag="PLR13_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19568.1"
                     /translation="MKKVVILHSDIATDGSLDELDCLVQAEAIADAVRTLHYEPVLLP
                     FELNLNRTITMLQSLKPAVVFNIVETLDSKGSLIHFAPAVLDVLQIPYTGCPTHAIYL
                     TSNKPTAKKIMRDAGIATPDWLEQNGIIFRQDDGGKFLIKSSWEHASIGLDENSLISY
                     TDKTAIIKEMKLREKKLGGSCYAEAYIDGREFNVALMSDQAGARVLPIAEMLFQDYAP
                     DKLKIVDYKAKWDADSFEYNNTVRKFNFDDEDALLISSLQDIALQCWNIFSLRGYARV
                     DFRVDKNNKPWVLEVNTNPCLSPDAGFAAALERAEIKYHEAIGLIIDNALKSISKEI"
     gene            complement(3823..4842)
                     /locus_tag="PLR13_09260"
     CDS             complement(3823..4842)
                     /locus_tag="PLR13_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19569.1"
                     /translation="MSRVSKKLKIGLTYDMRDDYLKEGYGLEETAEFDLPETIEAIER
                     VILDNGFQADRIGNIKALSRRLVAGDRWDLVFNIAEGMYGFGREAQVPALLEAYNIPY
                     TFSDPLGNAITLHKGFTKQILRDAGIPTPAFVVVDTAAHIDTISLPFPLFAKPVAEGT
                     GKGITALSKITSRKNLRAVCLNLLKTFRQPVLVETYLPGREFTVGILGTGKDARALGV
                     LEVILKPEAEKNAYSYENKEHYDKLVDYVLVDDPEARKAADISLMAWRALDLRDAGRV
                     DLRSNTYDVPHFMEVNSLAGLNPVRSDLPILCSKIGMSYHELICAIIKSALQRVKNHP
                     ENRCK"
     gene            complement(4881..6188)
                     /locus_tag="PLR13_09265"
     CDS             complement(4881..6188)
                     /locus_tag="PLR13_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HQC19570.1"
                     /translation="MESETLFKEEEEPPGHLTGLVHVKNNVSDFLLFKDEVLPYPVKQ
                     KSENVLFPFKDSARSRLFRSQFYPEITRFEWFDWRWQLRNTIRDADRLAQIIHLSDNE
                     HQAMAHHSGALPVSITPYYASLLNPDDPADALRRCVVPVVDECLHTEGEEEDPLCEDA
                     DSPVPGIIHRYPDRVLFLVTDICSTYCRYCTRSRIIERSTHHLINLEKWENAIQYIAG
                     NKNIRDVLLSGGDPLTLSDDKLEWLLARLHKIRHVELIRIGTKVPVVLPQRITPRLTA
                     MLKKYHPLWMSIHITHPRELTPEMSAACIRLANAGIPLGSQTVLLSGINDDVATMTRL
                     VQGQLQIRVRPYYLYQCDPIPGSSHFRTPISKGIEIIQGMRGHTTGYAVPTYVVDAPG
                     GGGKIPLLPEYAIGWENGSLLLRNYEGNIFRYPDNHAKSKDMH"
     gene            6704..7477
                     /gene="mutM"
                     /locus_tag="PLR13_09270"
     CDS             6704..7477
                     /gene="mutM"
                     /locus_tag="PLR13_09270"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HQC19571.1"
                     /translation="MPELPEVETLCRQLQKKIAGRSITGGKVYDDKLSAVQSFKKGKA
                     INVQRQGKTIVLELDNGRAINIHLRMTGRLFWREVSLRPDYARWRLSFDKGYLYLVDP
                     RRFATVTIGDIKKDTIKNDLMKDFDEKEFMSRQAKRSVSIKTLLLDPKAVAGIGNIYA
                     SEILYASGIHPFRKASDLSEGEWEKIITSSRQILKKGIEKRGTSISDWRDLYGRPGDN
                     QHALKVYGREGTHCSKCGGEICRIKQAGRSTFYCPDCQK"
     gene            complement(7490..7999)
                     /locus_tag="PLR13_09275"
     CDS             complement(7490..7999)
                     /locus_tag="PLR13_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQC19572.1"
                     /translation="MAENNISQIRINGRLVGIADLDHAIAKAARLDSKDDIDIKKCLL
                     NEISVNNYIPPSAQNAYADALLREFKIAHGLPVAEELFEGMNIEVVGMGCARCNQLEI
                     DVRDLLSEMNITAGLRHVTDFKEIARYNLLGSPALVINNKVVSVGDVPPKSKIRQWII
                     EAHQRTGKD"
     gene            8188..8781
                     /locus_tag="PLR13_09280"
     CDS             8188..8781
                     /locus_tag="PLR13_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC19573.1"
                     /translation="MDKPLMPQKLKQRLYPVVLRQFAGKDFHAVNIRDISKESGISTG
                     TIYKYFLSKEDLLFSIIDEKLLDLASLLRMHLEGTEDIREMFRKLFWVTMNFFDNNPD
                     LAVTAFITVPSKSFMASKSYKRESEIDILNGVVEKAKKRGAVISEIENRYFADLYMMI
                     TQRHIQNWYVHGMKWKLADTINQFFDFFWRVVKPIST"
     gene            8830..11019
                     /locus_tag="PLR13_09285"
     CDS             8830..11019
                     /locus_tag="PLR13_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC19574.1"
                     /translation="MVKIDILEKAKSEKRNTLTEAEAKEVLKQYGIPVVQEKAVKTIA
                     EAETESATIGYPVVVKGLGAKLTHKTEKGLVKLNLNTREDVRNACIYIKEAAGDDLEG
                     FLIQPMLDGKREFVAGLFRDEQFGPTVMFGLGGIFTEALGDVVFRIVPFDEKEALKMI
                     DELNAQKLLGAFRGENAPDKKALVKTLVGLSRIGLEIPEIKEIDINPLLVSADGKVTA
                     VDALIVLGKAAERKKMVRQVDLKHIWKLFHPRSIAFVGASSKIGKWGHIMYAGVVAGK
                     YQGAIYLVNATGGEIAGRKVYSSVAEIPESVDLAVVTVPADKILSLIPQFKEKGINNV
                     LIITSGFSEVGSEGKILEEELVKKAREAGIVIVGPNTMGICNPYISFYCIGSHVRPPA
                     GGTSLLAQSGNLGTQLLAFAAKEDIGIRAYCGSGNEAMLTIEDYMDGFEVDEKTKNIV
                     LYIESIKNGQRFVETARRVGKHKPVIVLKGGRTKAGSSAAASHTGAMASDIKVFTAAC
                     KQAGIIQAESPMDLLDLSAAFSSLPLPKGSSVALLTLGGGWGVVASDLCEENNLIVPK
                     LSEDIIAEINPLLPPFWSHANPIDVVGDMNSETYMKILESLSRWSGCDAIIHMGIIGR
                     KVMIRSIMESTAAVDNKYDSNFVEGSLQYIEAFEKRTIEQTVQLMEKYNKPIVGVYLL
                     SDDQTRTVTNVNGCKYKGVNFVTPEKAVKALAKMYQYSKWLKCENEN"
     gene            complement(11123..12544)
                     /locus_tag="PLR13_09290"
     CDS             complement(11123..12544)
                     /locus_tag="PLR13_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQC19575.1"
                     /translation="MAFLIAFFIVSFVEVLRSSLAPSGAPEIPAASALEGTTPGSFSD
                     LTERVKPAVVNISTTKTYKGRTLGNPFGRSPFNDYFGDDFFNRFFGDIPQQDLKQRSL
                     GSGFIISNDGYIFTNNHVVENTDKILVKISDGKEYEAKIIGTDAKTDIALIKIKPDNG
                     LPTVEIGDSDAVRVGEWVIAIGNPFGLEQTVTAGIVSAKGRVIGAGAYDNFIQTDASI
                     NPGNSGGPLFNMQGKVIGINTAIVAQGQGIGFAIPINMAKNILNDLKTKGKVTRGWLG
                     VSIQNISEDIAKSMNYKGKSGVLVSDVFKGDPADRAGIKVGDIIVEINGKSIKDTHEL
                     LLTTAGLHVGEKAIIKVIRDGREMSFSIIVAERKDKPETALTQKSGGYFGIHAQEITK
                     DMAKQFGIAANTGVIVTDVEGGSPADNVGIQTNDVIIQVNKIKIISLKQYLAEISKAA
                     EKKNVVLLVKRGNVNFFVGLSVE"
BASE COUNT         3713 a         2713 c         3076 g         3539 t
ORIGIN      
        1 cgggcattgt cccggatggc tttttcgtca taaatatgaa agggcgtcgg ataatcttta
       61 atgatatttt cgatttgctt tttcgaaaaa ggtaaaatct tttgaggcat aacagtcttc
      121 ctggataaaa ataatttacg tgaaatcaag cgtgttgtta aatttacggc aaaaccggaa
      181 aattatcaat actattttga aacgggacat atctgttatg tgataaagag ttggacacaa
      241 agatagatga tcgaggagag aatcgcggca gcgggaatgg tcagtatcca ggcccagata
      301 atttttccgg caacacccca acgcacagcg gaaagtcttc tcgtggaccc gacgccgacg
      361 atcgcgccgg taatggtatg ggttgtactt acgggaatac cggcaaccgt agcgccgata
      421 atcgagcagg ccgccgccgt ctccgcactg aatccgccca tcggctgaag cttggttatt
      481 tttgttccca tcgttttcac gattcgccat ccgccgaaca tggttcccag tgaaatcatg
      541 gaatagcagg caataatgac ccaaacagga atgtaaaacg tatcacccag caatcccttg
      601 ctgaacagtg ccatggcgat gattcccatg gttttttgcg catcgttcat accgtgtccc
      661 aacgaataca ccgcggcaga taaaagttga agtttccgga atagtttgtc ggatttgccg
      721 atgttggagt tgtggctcaa attcatggcc gtgaccataa aaataaaacc cagcgccatt
      781 ccgatggccg gagatacgat gatgaaaaca gtcgttttga tgatgcctgc ccataccagg
      841 gtgctgaagc cagctttcga gatggcggct ccgattaatc cgccgatgag tgcatgagac
      901 gaactgctgg gtaaaccata gtaccatgta atgatgttcc agataatcgc tccgcctaac
      961 gcggcaagga tgaccagatt atcaacaatg tcaatattaa taattccttt acctaccgtg
     1021 tgggcaacag ctgttcccac aaagaagacg gcggcaaagt tgaaaaaggc ggcccagatg
     1081 acggcgtatt tgggtgagag aacgcgggta gaaacgacag tggatatgga attggcagaa
     1141 tcatgaaaac catttagaaa atcaaaggca agcgcaccaa tgatgagaaa aacaacaaac
     1201 gccattgaat caagcatgtt tcaggacgat cccctccacg atgttggata cgtcttcgca
     1261 gacatcaatt gcttcttcaa ggagttgaaa aatatctttc cattttacaa gctcaatggc
     1321 gtctttttcg gtttcgaaaa gattggccat aatggacctt agcgtgatat ctccggcatt
     1381 ttcaagggta tttatttcca cgcaaccggc aagaatcatg tcggagtttt tcatgttccg
     1441 catggcctga ataatggttt tcagcttttt gatcgcatca ttcaggattt gtgcctgttt
     1501 gatgatttca tccgacgttc gtttgacttt atacagatga atccggacgg ctgttgcttc
     1561 aatgacatcc ataatgctgt ccattttatt gaccagtgcg taaatatctt cccggtcaat
     1621 aggcgtaagg aatgtcttgt gcattttttc gtatgtttta tgggtgatgc cgtcggcttc
     1681 gtgctcaatt tccttgagtt tggcgacccg cacatcggaa taatcccggg tctgagccat
     1741 ttccagaaac agcttgccgc cttcctcaat tttatcggca agttcatcaa aaagttcaaa
     1801 aaaattttct tctttgggga acaagcgcat gagataaacc tccttgcgtt cctggtgtaa
     1861 tccgtgctga cggaacgtca gcaaattact ggcagtgctc tagcgaatta tcaggaaaaa
     1921 tgcaagaatt aaaaccgcca gaacaaagaa gaaaggaatt aaatcctttt atttaagcac
     1981 cttagcgtaa ataatttttc cgtccccttc cgagtaaaaa ttcttcagaa aagcttcctt
     2041 ggcataaccg caggactcat aaaatttatg ggtgggcgtg tattgtgccc gcgaggaggt
     2101 ctccgcgtat atttgcctgc cgccgttttg ataaatgcgg ttttctgttt cggccatcag
     2161 catttttccg actcccagcc cctgtaaatc tttggcaacg acgatccagt aaagatcgta
     2221 gctcccggcc gtggccggta tgaggccgta gcacgtatag ccgacgacct ggttttcaat
     2281 ttcggcaaat aaaaactggt aagaactgac ctcctgtttg tccagtttat cttcagccag
     2341 ttcgatggct atttcgattt cttcatcaga aaagaagccg ctggattcca ccatatcggc
     2401 aatgacttcg atgtcggaag gtttaatttg ctggcgatag ataaattttt tcatattttg
     2461 catcacgtat gcgggaggcc ttcacgaaac atgaagtaaa ccgctccgac taggcaaagg
     2521 ccggcccaca gaaaatccat cttcaacggt tgtttcatat acatgatcgc aaaaccggca
     2581 aatacaatca tggtaatcac ttcctgaatc actttaagct gtcccaggct gtaataatga
     2641 aaaccgatac gattggcggg aacctgtaaa acatattcaa aaaaggcgac gccccaactg
     2701 aacaagatga caaacaataa aggcttgtgg ttgaagtttt tcagatgtcc gtaccaggca
     2761 aacgtcatga ataagttgga aaagaaaagg aatatgatgg tttttaacac agatattaga
     2821 tctccttgga tatagatttc aacgcgttat caataattaa accgatagcc tcgtgatatt
     2881 taatttcagc cctctccagc gccgcagcaa aaccggcatc cggcgacaaa cacggattgg
     2941 tgttgacctc caatacccag ggcttgttgt tcttgtcaac acgaaagtca acacgcgcgt
     3001 acccgcgcaa cgaaaaaata ttccagcact gcagggcaat gtcctgtaag gacgatatga
     3061 ggagggcatc ctcgtcatca aaattgaatt ttcgcactgt attattgtat tcaaaggaat
     3121 ccgcgtccca tttggccttg taatcaacaa ttttcaattt atccggagca taatcctgaa
     3181 acagcatttc cgctatcggc aaaactctgg ccccggcctg atcagacatt aaagcaacgt
     3241 tgaactcgcg gccgtcgatg taggcttcgg cgtaacaaga gccgcccaat tttttttcgc
     3301 ggagtttcat ttctttgatg atcgcagttt tgtccgtata ggaaatcagt gagttttcat
     3361 caagaccgat cgaggcatgt tcccaggacg acttgattaa aaattttccg ccatcatcct
     3421 gtcggaagat gatgccattc tgttccagcc aatccggcgt ggcaatgccg gcatcgcgca
     3481 tgattttttt ggccgtcggc ttgttggacg tgaggtatat ggcgtgtgtc gggcaaccgg
     3541 tataaggaat ctgcaacaca tcaagaacag cgggagcgaa atgaatcagg ctgcctttgg
     3601 agtccagggt ttctacaata ttaaaaacga ccgccggttt caatgattgc agcatggtta
     3661 tggtacgatt caggttgagt tcaaaaggca gcaaaaccgg ttcataatgc agcgtgcgca
     3721 cggcgtcggc aatggcttcg gcctgcacga gacaatccag ttcgtccagg ctgccatccg
     3781 ttgcgatatc actgtgcaga ataactactt ttttcataaa aattatttac accggttttc
     3841 aggatgattt tttacacgtt ggagcgccga tttaatgatg gcgcagatca gctcatgata
     3901 gctcatgcca attttagaac acaggatggg caaatccgaa cgcaccgggt tgagaccggc
     3961 cagcgaattg acttccataa aatggggaac atcgtacgtg ttggagcgaa ggtcaacacg
     4021 accggcatct cttaaatcca atgccctcca ggccattaat gaaatgtccg cagcctttcg
     4081 ggcctctgga tcatcgacca ggacatagtc aaccagtttg tcgtagtgtt ctttgttttc
     4141 gtaggagtag gcgttttttt cggcttccgg ttttaaaata acttccagaa cgcccaaggc
     4201 gcgggcgtct ttgcctgttc ccaggatacc gactgtgaat tcgcgtcccg gcagatacgt
     4261 ttccaccagc acaggctgcc ggaaagtctt taaaagattc aggcagacgg cacgcaggtt
     4321 ttttcggctg gtgattttgg atagggctgt gatgcccttt cccgttcctt ccgccaccgg
     4381 tttggcaaaa agcggaaagg gaagggagat tgtgtcgatg tgagcggctg tgtcgaccac
     4441 gacaaaagcg ggagtgggaa tgccggcatc acggagaatc tgtttggtaa agcccttatg
     4501 cagcgtgatg gcgttgccca gaggatcgga aaaggtgtag gggatgttgt aggcttccaa
     4561 aagagcgggg acctgtgctt cccggccgaa accgtacatg ccttcggcaa tgttgaaaac
     4621 cagatcccag cggtctcccg caacaagcct tcgggacaga gctttaatat tgccgatccg
     4681 gtcggcctga aaaccgttgt ccagaatgac gcgttcaatg gcttcgatgg tttcgggaag
     4741 atcgaattca gccgtttctt cgagaccgta tccttccttc aggtaatcgt cgcgcatatc
     4801 ataggtgaga ccgattttga gtttcttgct gacgcgactc acggttgaat tttatacccc
     4861 ctgtcgtcaa gactgcctgt ttagtgcata tctttcgatt tggcgtggtt gtcgggatag
     4921 cgaaagatat tgccttcata attacgaagc aacagggagc cgttttccca cccgatggcg
     4981 tattcgggca gcaaaggaat cttgccgccg ccgccgggtg cgtcaacaac ataagtcggt
     5041 acggcgtagc cggtggtatg tccgcgcatg ccctggatga tctcgatgcc tttgctgatc
     5101 ggagtccgga aatgggaaga gccggggatg ggatcgcatt gatacagata ataaggacgg
     5161 actctgattt gcagttgacc ctgtaccagt ctggtcatgg tggcaacatc atcgttgatg
     5221 ccggagagca gaacggtctg gcttccgagc ggaataccgg cattggccag acggatgcag
     5281 gcagcgctca tctccggcgt cagctctctg ggatgggtaa tatgaatgct catccatagc
     5341 ggatggtatt tcttcagcat ggccgtcaga cggggagtga tcctttgcgg aagcacaacg
     5401 ggaactttgg tgccgatcct gatcagttcg acgtggcgaa tcttgtgaag acgtgccagc
     5461 agccattcaa gcttgtcgtc ggacagggtt aaggggtcgc cgccggagag caacacatcc
     5521 cggatgttct tattaccggc gatatactga atggcgtttt cccatttttc aaggttgatg
     5581 agatgatgcg tgctgcgttc gataatgcgg gaacgtgtgc agtagcggca gtaggtggag
     5641 cagatgtcgg tcactaaaaa caaaacgcga tccggataac ggtgaatgat tcccggaacc
     5701 ggcgaatccg cgtcttcaca gagaggatct tcctcttccc cctccgtatg caggcactca
     5761 tccaccaccg gaacaacaca tcggcgcaga gcgtcggcag gatcatcggg attcagcaga
     5821 ctggcatagt aaggcgtaat ggaaacaggc agggcgccgg aatgatgcgc catggcctgg
     5881 tgttcgttat cggagagatg gatgatctgc gcgagcctgt cggcatcccg gatggtgttg
     5941 cgcagttgcc agcgccagtc gaaccattca aaacgggtaa tctcgggata aaactgtgat
     6001 cggaagaggc gggagcgggc agaatcttta aaaggaaata aaacattctc tgacttttgt
     6061 tttaccggat aaggaagaac ctcatctttg aataacaaaa agtcactgac attgttttta
     6121 acatgaacaa gaccggtcaa atgaccagga ggttcttcct cctctttaaa cagggtctcg
     6181 ctttccattt gtgcctcctt gatgaggacg cccctccctt aaaaaaaatt ttaaaataaa
     6241 aatctctttg attatgaaat gtactcatac aaaaatattt gtcaacattt ttttgcaatt
     6301 ttcaaaaaaa attttaaaaa agttttcaga cgaaacacgt tcagaggaaa agcttccgca
     6361 tgatggcgac aggttttgaa ggaactgccc tcgaaaagcg gttggatgtc cagaacgatg
     6421 agaaaggttg aatactgatc tggaggccgt caaatcaggc tcagcgcagg ataggcaatt
     6481 tataaaaaca tccacgatca gctcgcatgc agcaaagacg attatcagaa atggcgtgtt
     6541 tcataaaaat cgttcagcca tgcgcacagg agggaggggt tgcctgacag ggcgatctgt
     6601 gtcatggtgc agcagaaaac gaataaaaaa atattcctgc gacgcagaat ccattggtct
     6661 gtccggatat gaaagtaatg aatgttcaaa aaggagacga ggtatgcccg aattacccga
     6721 agtagagacg ctctgccggc agcttcagaa aaagattgcg ggcaggagca ttaccggagg
     6781 taaagtgtat gacgataaat tgtccgctgt ccaatcgttt aaaaaaggaa aggcgataaa
     6841 cgttcaacgg cagggcaaga ccattgttct agagctcgat aatggccgtg cgataaatat
     6901 ccatttgcgc atgacggggc gattgttctg gcgggaggtt tcgctgcgac cggattatgc
     6961 ccgctggcga ttgtcgttcg ataaagggta tctgtatctg gttgacccgc gacggttcgc
     7021 cacggtaacg atcggcgata taaaaaagga tacaataaaa aacgatctga tgaaagattt
     7081 tgatgaaaag gagtttatga gcagacaggc gaaacggagc gtttctatca aaacgctgct
     7141 tctggatccg aaggcggttg ccggtatcgg caatatttac gcctctgaaa ttttatatgc
     7201 ctccggcatt catcctttca ggaaggcgtc ggatttgtca gaaggggaat gggaaaaaat
     7261 tataacgtcg tctcggcaaa ttttaaagaa gggaattgaa aagagaggca cgtcgatttc
     7321 agattggcgg gatctttatg gtcgcccggg tgacaatcag catgcattga aagtctacgg
     7381 tcgggaaggg acacactgtt caaaatgcgg gggagagatt tgccggatca aacaggcggg
     7441 gcgaagcact ttttattgtc ctgattgcca gaaataaaga aagattagtc taatctttgc
     7501 cggtgcgctg gtgcgcttcg ataatccatt gacggatttt gcttttcgga ggaacgtcgc
     7561 cgactgaaac aaccttatta ttaatgacca aagcggggga gccgagaaga ttgtatcgcg
     7621 caatttcctt aaaatcggtg acgtgcctga ggcctgcggt aatgttcatc tcggacaata
     7681 aatcgcgcac atcaatttcc aattgattgc agcgtgcaca tcccataccc accacctcga
     7741 tattcatccc ctcaaatagt tcctcggcaa cgggcaaccc gtgggcaatc ttaaattctc
     7801 tcaacagcgc atcggcataa gcgttttgtg ctgaaggagg aatataattg ttcacggata
     7861 tttcattaag aagacatttt ttaatgtcga tatcatcctt gctgtcgagc cgggcggcct
     7921 ttgctatcgc atgatccaaa tccgctatgc caacaagacg gccattgatc ctgatttgcg
     7981 aaatattatt ttctgccatg agcagcgtcc cagtttttta taaatttatt tgttcgcaaa
     8041 gacaacgtca agcaggaaat gtattttttg gacaaatatt aacattatca tattaaaata
     8101 aaacaatgtt ttaaaaaata cttgacaaat gaaacaatgt tttatatttt gccggtattc
     8161 aaaacatata tataaggaag ggtggtaatg gataaacctt taatgccaca gaaactgaag
     8221 cagcggcttt atcctgttgt attgaggcag tttgccggaa aagattttca tgccgttaat
     8281 attagagaca tctcaaagga aagcggaatc agcacaggaa caatttataa atatttttta
     8341 tcgaaagaag atcttttgtt ttccatcatt gatgaaaaat tattggacct ggcgtcctta
     8401 ttaaggatgc atcttgaggg aacggaggat atccgtgaaa tgtttcgaaa attgttctgg
     8461 gtgacgatga atttcttcga taataatccg gatctggcag ttactgcttt tataacggtt
     8521 ccgtcaaaaa gtttcatggc cagcaaatca tataaacgcg aatcggaaat agacatcttg
     8581 aatggtgttg tggagaaagc aaaaaagcga ggtgctgtta tatcggaaat tgaaaaccgt
     8641 tattttgcag atctttatat gatgattacg caaagacaca tacaaaactg gtatgtacac
     8701 ggtatgaaat ggaaacttgc ggatacgata aatcagttct ttgatttctt ctggagagtc
     8761 gtaaaaccga taagtacata aaaatattaa taaataataa aatcagcatg cggtaatacg
     8821 gagtcaataa tggtgaaaat cgatatcctc gaaaaagcaa aatcagaaaa aagaaataca
     8881 ttaacagaag cggaagccaa agaagtatta aaacaatatg gtatcccggt tgttcaagaa
     8941 aaagcggtta aaacaattgc agaagcagaa accgaatcgg caacaatagg atatcctgtt
     9001 gttgtgaaag gattgggagc aaaattaacg cataaaacgg aaaaaggtct ggttaagctg
     9061 aacttgaaca cgagggaaga cgttcgaaat gcgtgcattt atataaagga agcagccggt
     9121 gatgatttgg aaggattttt aatccaaccc atgttggacg ggaaaaggga gtttgtcgcc
     9181 ggactttttc gtgatgaaca gtttggccct accgtcatgt ttggtttggg aggaattttt
     9241 acagaagcac tcggcgatgt tgtttttcgt atagttcctt ttgatgaaaa agaagcgttg
     9301 aaaatgattg atgagttaaa tgcgcagaaa ttgcttggag cgtttcgggg agaaaacgct
     9361 ccggataaaa aagctctagt gaaaacactg gtcggtttgt cgcgtatcgg tttagagata
     9421 ccggaaatca aggaaattga tattaatcca ttattagttt ctgcagacgg aaaagtgact
     9481 gcagttgacg cattgatcgt tttaggaaaa gctgcggaaa gaaaaaaaat ggtccgtcag
     9541 gttgatttaa agcatatctg gaaattattt catccacgat caatagcgtt tgtgggtgct
     9601 tcttcgaaaa tcggcaaatg ggggcatatc atgtatgccg gcgttgttgc cggaaaatat
     9661 cagggagcca tttatcttgt taatgccaca ggaggagaga ttgccggtag aaaggtttat
     9721 tcgtcggttg cggaaattcc tgagtctgta gatcttgccg tcgttaccgt tcccgctgat
     9781 aaaattttat ctctgattcc gcaattcaag gaaaaaggca taaacaatgt tttaatcatc
     9841 acgtcgggat tcagtgaagt aggttcagaa ggaaagattt tggaagaaga actggttaag
     9901 aaggcgcgag aggccggaat agttattgtt ggtccaaaca cgatgggcat ctgtaatcca
     9961 tatatatcat tttattgtat aggttcgcat gtccggccac cggcgggcgg gacctcattg
    10021 cttgcccaat ccggtaatct gggaacgcaa ttgctggctt ttgccgcaaa agaggatatt
    10081 ggaataagag cctattgcgg ttccggaaat gaagcaatgt tgaccattga agactatatg
    10141 gatggattcg aagttgacga gaagacaaag aacattgtct tatatattga aagcattaaa
    10201 aatggtcaaa gatttgtaga aactgccaga cgtgtgggca aacataaacc ggttattgtt
    10261 ttaaagggcg gacgcacaaa ggccggatcc agtgctgcag cgagtcatac cggtgcaatg
    10321 gcttccgata ttaaagtgtt tacagcagcg tgtaaacagg ccggcattat acaagccgaa
    10381 agcccaatgg atttactgga cctttcagca gcattttcct cattgccgtt gcccaaaggc
    10441 agtagtgtcg ctcttttaac gttgggcgga ggatgggggg ttgtcgcctc cgacttgtgc
    10501 gaggaaaata atttgattgt tccaaaatta tcagaagaca tcattgctga aattaatcca
    10561 ttattgccgc ccttctggag tcatgccaat cccatcgatg ttgtaggcga tatgaattcg
    10621 gaaacttata tgaaaattct ggaaagcctg agtcgatggt ccggatgtga tgccatcatc
    10681 catatgggga ttatcggcag aaaggtgatg atcagatcga ttatggagtc cactgccgcg
    10741 gtggataata aatatgattc taactttgtt gaaggctctt tacagtatat cgaggcgttt
    10801 gaaaaaagaa ccatcgagca gacggtacaa ttaatggaaa aatataataa gccaattgtt
    10861 ggggtttatt tgttgagcga tgatcaaacc aggacagtta caaatgtaaa tggatgtaag
    10921 tataaagggg taaactttgt gaccccggaa aaggctgtca aggcattggc aaaaatgtat
    10981 caatattcaa aatggctaaa atgtgaaaat gaaaattaac atcatggagg acaagaaatg
    11041 tttcacgtga aacatttttg ccctgtaaca aaaaaaggca ggctttccat agcctgcctt
    11101 ttttaatatc atataaatta tattattcta cgcttaatcc tacaaagaag ttgacattgc
    11161 ctcgcttcac taaaaggacc acattcttct tctctgcagc tttagatatt tcagccagat
    11221 attgtttgag tgaaatgatt ttaattttat taacctgaat aatcacatcg ttggtctgaa
    11281 ttccgacatt atcagccgga ctgccccctt ctacatccgt aacgatgaca cccgtattgg
    11341 ctgcaatgcc aaattgtttg gccatgtctt ttgtaatttc ctgggcatga ataccgaagt
    11401 acccacccga tttttgtgtc aaagctgttt cgggtttgtc ttttctttcc gccacgataa
    11461 ttgagaagga catttctctt ccatcacgaa taactttgat aattgctttt tctcctacgt
    11521 gcagaccggc agtggttaac aatagctcat gagtatcttt gatgctcttt ccattaattt
    11581 ctacaataat atcaccaact tttataccgg ctcgatctgc cggatcgcct ttgaaaacat
    11641 cagaaacaag cacaccgctt ttgcctttat aattcatgct ctttgcaatg tcttcggaaa
    11701 tattctgaat cgaaacccct aaccatccgc gggtgacttt tccttttgtt tttaaatcat
    11761 tcaaaatgtt tttggccata tttatgggaa tcgcaaagcc aatgccctgg ccctgtgcaa
    11821 caatggctgt attgatgccg atgactttcc cctgcatatt aaataaaggt cctccgctgt
    11881 ttccgggatt gatggaggca tccgtctgaa tgaaattatc gtaagcacct gcgccgatca
    11941 cccttccttt ggcgctgaca attccggccg tgactgtttg ttcaagtcca aaaggattgc
    12001 cgatggcaat aacccattct cccactctta cagcatccga atctccgatt tccacggtcg
    12061 gcaatccatt gtccggttta atttttatta aagcaatgtc cgtctttgcg tccgtgccga
    12121 tgatttttgc ttcatattct tttccatctg atatctttac gagaatttta tcagtatttt
    12181 caacaacatg gttatttgtg aaaatgtaac cgtcattgct gataataaaa cctgatccca
    12241 ggcttctctg cttgaggtcc tgctgcggaa tgtcgccgaa gaaacgattg aagaagtcgt
    12301 ccccaaaata atcattaaaa ggagaacgcc caaacggatt acccagggtt cttcctttat
    12361 aggtttttgt cgtgctgatg tttacaacag ccggtttgac tctttcggtc agatcggaaa
    12421 atgatccggg tgtcgttcct tccagtgccg atgcggctgg aatttccggt gcgcccgaag
    12481 gggcaagaga cgatcttaac acttctacaa atgagacaat aaaaaaggcg atcaaaaacg
    12541 ccatcaaaaa catgaaaaac gttttacggt tcttattttc catattaatg ttataaacct
    12601 ccgttattat tggtcaaata ttattgttga tgatagattt acgcgatata ttaaatttat
    12661 actttttaaa caatataact gcaaagatgt ttttgtcaat aaaatattgt ttaatatggg
    12721 tcagcaagaa acatcttttc aaacgttttg ggtttaaaaa aacaaacgga tcattatctg
    12781 ccgtcggcga aaatattttg ctgtataaaa ttagtccatc tgaagaaaat ttttttatag
    12841 attttaattg ttagagattt aaatcaaatc aaaaatttct atagatattt gaatcgtatg
    12901 ccagcaaatc cttgacagtg aatttcaaaa ataatataag cttgacgaat ttttaaatta
    12961 taaaattcat gcctgggtgg cggaactggt agacgcaagg gacttaaaat ccctcggtcc
    13021 ttgaggctgt gcgggttcaa t
//
LOCUS       DAONQZ010000064        12787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5426_length_12787_cov_12.911247, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000064 DAONQZ010000000
VERSION     DAONQZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_12787_cov_12.911247"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..138
                     /locus_tag="PLR13_09295"
     tRNA            63..138
                     /locus_tag="PLR13_09295"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Cys,seq:gca)
     gene            362..847
                     /locus_tag="PLR13_09300"
     CDS             362..847
                     /locus_tag="PLR13_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQC19576.1"
                     /translation="MFKVGDLAVYPAHGVGVIEAIENKEVMGKKQSFYVMKIMGNGMK
                     IMIPTMSVKAVGLREIIMEKEIPKVYEILRNKDVTIDKQTWNKRYREYLERIKTGSVF
                     EIAGVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQESEVEHNLQKIFTV
                     Q"
     gene            1010..2011
                     /locus_tag="PLR13_09305"
     CDS             1010..2011
                     /locus_tag="PLR13_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQC19577.1"
                     /translation="MKRLIIRIILIMACSISGYAIAFYTSSDSFLILKSIAGFIIGLT
                     IALTVIKIEQHIRQLSLKIIIGGVAGMIVGLLIALIFSFGLNFVSKIRENQQALPWIY
                     LLITGIFSYLGLTLGSKKIEEFSFGGIDSKTAHDHRILDTSVIIEGRIADICDSGFIE
                     GELIVPRFVLNELQQVADSSDSMKRLRGRRGLDVLNHLQKSANVNIEIVDQDFPKIKG
                     VDGKLVALAKQLNAKLVTNDYNLNKVAELQGIRVLNINELANAMKPVVLPGEQMTVKI
                     VREGKEAGQGVAYLDDGTMIIVDNAQKMLNMNVRVVVTSVLQTTAGRMIFSELKEVA"
     gene            2107..>2627
                     /locus_tag="PLR13_09310"
     CDS             2107..>2627
                     /locus_tag="PLR13_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQC19578.1"
                     /translation="MKNNGEQSMKTVLIIPAGGAGQRFKSHVAKQYLLLDALPVLVHT
                     LKVFQDSPVVDEIILVLPQDDVVHVRQDLIEKYGMSKVVAVVAGGRERQDSVKNGLDA
                     IQGNAGLIIVHDAVRPFVTDQMIGRVVAAARACGAAVTGVQVKDTVKATKDDRMIQST
                     VPRENLWLTQTPQA"
     assembly_gap    2628..2727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2728..3126
                     /gene="ispF"
                     /locus_tag="PLR13_09315"
     CDS             <2728..3126
                     /gene="ispF"
                     /locus_tag="PLR13_09315"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQC19579.1"
                     /translation="GHSDADALIHAICDALLGAAGAGDIGRHFPDTDPQYKDISSMIL
                     LKNVKKILDGKGFRIENIDATVILEVPRLAPYADAIVANIASVLNISEECVNIKAKTN
                     EGMGFTGRREGVAVFAVASLAERMRNGAKT"
     gene            3110..4549
                     /gene="gltX"
                     /locus_tag="PLR13_09320"
     CDS             3110..4549
                     /gene="gltX"
                     /locus_tag="PLR13_09320"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQC19580.1"
                     /translation="MVRKPRVRFAPSPTGELHVGNARTALFNWMFARHHGGSFVLRIE
                     DTDASRSALSYQTNLYDDLKWLGLDWDEGPEKGGHFGPYRQSERVAVYREFLQKLMKA
                     GAVYPCYCTEEELEEERQTLILSKRMPRYMGKCRNLSPEERNRKEKEGRKPSYRFKVQ
                     SQIIEFEDLIRGNVKFNAEDMGDFIIVRSNGMPAYNFACVIDDHLMDMTHVIRGEDHL
                     SNTALQIMLYRAFGFDSPVFAHHSLILGRDRTKLSKRHGSVSIGEFRRQGILPEAMIN
                     YLGLLGSSFTGAREVLSRDDMIQDFSLERASRSGAVFDEEKLHWLNAIYIRNTRTDDL
                     VERLKPFLDEPAYQNEMQNPEWFRKAVELVKTDLTTLADLKNHISIFFDSQYRISAEA
                     KDLLDQEPARKVVEAFASYLEKQDAGNQNLYAEAVRFAREATGAKGRALFMPLRAALT
                     GLLHGPELDKIFDVLGRNAALRRLKYFER"
     gene            complement(4546..4938)
                     /locus_tag="PLR13_09325"
     CDS             complement(4546..4938)
                     /locus_tag="PLR13_09325"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HQC19581.1"
                     /translation="MSFNLSRDAVYHILADILVAVHFLFILFVVFGGLLVIWRPSFAW
                     AHLPAALWGAFVEIFGWICPLTPLENHLRTLAGESVYGGDFIARYLLSLIYPENLTPS
                     IQKGLGAVVIVINLIFYFIAFKKNRARR"
     gene            complement(4928..6043)
                     /locus_tag="PLR13_09330"
     CDS             complement(4928..6043)
                     /locus_tag="PLR13_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQC19582.1"
                     /translation="MKKIFAAVALIVVLLTAYLTLWPVPVDPVQWIPPPSDGYTGPHA
                     VNTGLAGLRMISLGREEGPEHIAIGKDQKLYATVAGGKILRMNQDGTQQEVYADTGGP
                     VLGFDFDARGNIVAADVDRGLVLISPEKNITVLTDNVNGDPIRFADAVAVSQSGKIYF
                     TDASTRFAPKQWGGIFPASMIDILEQRSTGRVLEYDPAAQTTRIVAKGFSFANGVVLS
                     EDENWLFVNETGKYRVWKISVTAQDLDIATPGKSAFILFDNLPGYPDNLMRGLHGKIW
                     LGFAKPRNPAIDQRVITSTPFMRKLTYRLPKFLWPLPKPYGHVLAFTEDGRIVADLQD
                     PGGRYPETTGVTETADRLYIQSLHAKGIGWLPKDNVF"
     gene            complement(6089..7660)
                     /locus_tag="PLR13_09335"
     CDS             complement(6089..7660)
                     /locus_tag="PLR13_09335"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQC19583.1"
                     /translation="MSEDVQKNSTQEKINHFEKKNRTLKQMGGEKQIARQHELKKLTA
                     RERLDLFFDQDTFQEVQLFVEHRSTLFGLDKKDIPADGVVTGFGKVNGRTVFVAAQDF
                     TASGGSLGEMHAKKIWKVMEMALAAQKPFVSLNDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALSDWVFMVKGSSFMYITGPDVVKAVIGEEISHD
                     ELGGALAHAAKSGVCHVVTESDTDCIEKIKKLLAYLPDSCHAPLPLSDCTDPADRTCP
                     ELDSMIPDKSNRAYDMKKIIKSVADNGELFELLELWAQNIITAFIRVTGRPVGVIANN
                     PRFAAGVLNVDASDKAARFIRFCDAFNIPLLTFTDVPGYMPGTHQEWAGIIRHGAKLL
                     HAYSEATVPKITVITRKAYGGAYIGMCSKQLGADYVMAWPTAEIAVMGAEGACNIIYR
                     NDIAGADNPTARRAQLVEAYEQQFNNPYFAASMGIIEEIIPPRDTRKKIIALLDALGN
                     KKETGLSKKHNNIPL"
     gene            complement(7820..8353)
                     /locus_tag="PLR13_09340"
     CDS             complement(7820..8353)
                     /locus_tag="PLR13_09340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC19584.1"
                     /translation="MSFKKPYPAQYEKFLALKNGKNVFVRPIRDTDGKLLLELFDKLY
                     PDSIYRRFLTPLRMIPENLLFKLTHVNYDSDFALVAVIPENGKDAIIAVARYGYDVRE
                     QVTDFAILVRDDWQGLGLGKRLLKDIFAIGRDHGVSCFVSLIDANNHVMKQLIKSLDY
                     PVTYSYRTGAAKVQIFV"
     gene            8686..>9335
                     /locus_tag="PLR13_09345"
     CDS             8686..>9335
                     /locus_tag="PLR13_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQC19585.1"
                     /translation="MEKLFYPESIAIIGLSSKPQNIPRLVLENLIRWGYKGRIFGINP
                     RATDSHVDGIKMYKSVRELPVVPDLVYVLIPARMIPDVMDSCGRAGVKRLAIPTGGFN
                     ELGEEGRKLSELVLQKAREYGIRFVGPNGVTVANTANGLCLPFVPSYSPPKGGLSIIS
                     QSGGVGLMLWNLMTDENVGMAKFASIGNKLDLDEVDFLEYFGRDPETKVIGMYLESV"
     assembly_gap    9336..9435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9436..9969
                     /locus_tag="PLR13_09350"
     CDS             <9436..9969
                     /locus_tag="PLR13_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC19586.1"
                     /translation="VVLKVVSPDAVHKSEAGGVITDVQNAEEAVKAFDQIRSNLFRYN
                     AHADFQGVRVMKQASDGYDMFIGGKFDESFGPVVFFGYGGIYIEVFQDTANLLCPADR
                     HEIEAGVQRLKSYNILQGMRGKGAGDIAGYVAMIERVSHLLHQFPQIRELDINPVRVL
                     ADGSGVLALDARVRIDT"
     gene            10008..>10651
                     /gene="truA"
                     /locus_tag="PLR13_09355"
     CDS             10008..>10651
                     /gene="truA"
                     /locus_tag="PLR13_09355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQC19587.1"
                     /translation="MRNFKITVEYDGAAYCGWQRQHNGIAIQQLLEEAIKKITGQNVS
                     VIGSGRTDAGVHAMNQVGSFKCDTTLPVQKIFMGVNSVLPPDIVVKNLEERSDDFHAL
                     RDVKSKVYVYRICNQRLRPVLGRNYFWHVRYPLDIASMRKAVRLIIGTHDFSCFCAAG
                     TDVKDRVRTIVDIEIMTGEEGLIEIQVESHGFLKYMVRNIIGTLIEVGRGKRQP"
     assembly_gap    10652..10751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10752..11470
                     /locus_tag="PLR13_09360"
     CDS             <10752..11470
                     /locus_tag="PLR13_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQC19588.1"
                     /translation="CETDRETCFAVNAEAVKNIAGACRQKKIRVLHFSTDYVFDGTAR
                     KPYREDALCNPVNLYGASKLAGENYLRTLSDNYLLIRSSWLYGINGKNFVQAILDKAK
                     AKEFIRDTMNRAKAVAEAPSTLSVVDDQVGSPTYTKDLALAVAFLIRKNATGIVHVTN
                     RGSCSWHQFAVRILKSAGYGHIVVEPIKSSELQRPAMRPAYSILSNKTFIEITGKAMQ
                     PWQLALEDYLNNFKLKKSHS"
     gene            11762..12691
                     /locus_tag="PLR13_09365"
     CDS             11762..12691
                     /locus_tag="PLR13_09365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19589.1"
                     /translation="MKKAMLVHLNELDFYLKKIDAVWPVRCLTGEAIDCEKVVDYYRQ
                     SRIVYKYVHSYSGAMHMAVNSDGVFNAKGFYTSLNEIVEIISPLPAGAVLELGCGKGF
                     NSAYLAKKIPHAHFTGIDVSGEHLAIARKKASRIHNLSILYGDFQDIMFPDGAFDTVF
                     ELESICHARKVRQVLSEVFRVLKKGGRFIVYDGFRQSGFHRLPDRLIEAARMTEKALA
                     VDHFEDIDGWLAMAVAEGFRIHEKKDLSASIMPNLARLQYLARRYFEYPMLTKLLLRL
                     LNVYTMRNCIAVLLMPFTLHNNAHGYYKIILEK"
BASE COUNT         3175 a         2929 c         3335 g         3048 t
ORIGIN      
        1 ctaccttgcc aaggtagacg tcgacggttc aaatccgttc gcccgctcca ttttattttt
       61 aaggcggcat agccaagtgg ctaaggcagc ggtctgcaaa accgttattc cccggttcaa
      121 gtccgggtgc cgcctccagg aaaattcagg agtttacttt ttttaaaaaa agtgactcct
      181 ttttattttt ataagttata tccgatattt aatcattcat gttgtgttta aaaaacggtt
      241 aaattatggt tttttagcca tgaaatattc aagtattact taaatacgta ttggttattg
      301 actaatagtt caaatagtgg tatgataacc ctctgatatg gaaaggcagg cgaaaaataa
      361 aatgtttaaa gtaggcgatt tggcggtata tccggcacat ggtgttggag tcattgaagc
      421 cattgaaaat aaagaagtaa tgggtaaaaa acagtctttt tacgtgatga agatcatggg
      481 gaacggcatg aaaatcatga ttcccacgat gagcgtcaag gccgtcggct tgcgggaaat
      541 catcatggaa aaggaaatac ccaaggtgta cgaaattctt cgcaacaaag atgtcaccat
      601 cgataagcag acctggaaca aacgttaccg cgaatatctt gagcgaatca agacgggttc
      661 ggtatttgaa attgcggggg ttctgcgcga tttgtttatt ttaaaaagcg ataaaaatct
      721 ttccttcggc gaacgaaaga tgatggatac agccaaaaat cttctggtca aagaaatatc
      781 cgtggccacc aacgcgcagg aatctgaagt cgagcataat ctgcagaaga tttttaccgt
      841 tcagtagaaa aatcatcttc atagtcaatc aggctctttg aaaattgaaa aaagacaggt
      901 tactttcttt ttttagggat accgtcattt ttcctccgtc cttatattat tttttaaata
      961 aattttttat aaacaaattt ttttaacatt catgattcac agaaaggagg tgaaacggtt
     1021 gattatcaga attatattaa taatggcatg ttccatcagc ggttatgcca tcgcctttta
     1081 tacgtcttcc gattcttttc tgatccttaa atccattgcc ggctttatta tcggattaac
     1141 gattgccttg actgtcataa aaatagaaca gcacattcgt caattatctc tcaaaattat
     1201 catcggcggc gtcgcgggca tgattgtcgg actcctgatt gcattaatat ttagtttcgg
     1261 attaaatttt gtaagtaaaa tcagggagaa tcagcaggcc cttccctgga tttatctgct
     1321 gattacagga atattcagtt atctgggcct caccctgggt tcgaaaaaaa tcgaagaatt
     1381 cagctttggc ggcatcgatt ccaaaaccgc ccacgatcac cgtattctgg ataccagtgt
     1441 catcattgaa ggacgcattg ccgatatctg cgacagcgga ttcatcgaag gagaattgat
     1501 cgttccccga ttcgttttga atgaactgca gcaggtggcg gattcgtccg attcgatgaa
     1561 acgtctgcgg ggaaggcgcg ggctggatgt cctcaatcat ctgcagaaaa gtgccaacgt
     1621 caacatcgag attgtcgatc aggattttcc caagatcaaa ggcgtggacg gaaaactggt
     1681 cgctctggcc aaacagctca acgccaagct tgtgaccaat gattataatc tcaataaggt
     1741 ggccgaactt cagggcatca gagtgttaaa catcaatgaa ctggccaacg cgatgaagcc
     1801 ggtggtgctc cccggcgaac agatgaccgt gaaaatcgtg cgagagggaa aggaagccgg
     1861 ccagggcgtt gcctatctgg atgacggaac catgattatt gtggacaacg ctcagaaaat
     1921 gcttaatatg aatgtccggg tggttgtgac cagtgttctg cagaccactg cgggaagaat
     1981 gattttttca gaactcaagg aagtggctta agccgaatgc gtcatcggtt tttttaaaac
     2041 ccgggatcaa tctcccgggt ttttttatcg ctattccatt ttgataataa aaatgctatc
     2101 agcttcatga aaaataacgg ggaacaatcc atgaaaacgg tgctcatcat tcctgccggc
     2161 ggggcagggc aacgatttaa atcccacgtc gccaagcagt atctgctgct ggatgcgctg
     2221 cccgtactgg ttcatacgct gaaggttttt caggactctc cggttgttga cgaaatcatc
     2281 ctggtgttgc cgcaagacga tgtggtgcat gtccgccagg acctgattga aaaatacggc
     2341 atgagcaaag tggttgccgt tgttgccgga ggccgggaaa gacaggattc cgtcaaaaac
     2401 ggccttgacg cgatccaggg gaatgccggg ctgatcattg tccatgacgc ggtgcgtccc
     2461 tttgtgacgg atcaaatgat cggcagggtg gtggctgccg ccagggcctg cggagcggcg
     2521 gtgaccggcg tgcaggtcaa ggacaccgtt aaggcaacaa aagacgatcg catgatccaa
     2581 tccacggtgc caagggaaaa tctgtggctg acgcagaccc cgcaggcnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc cattcggatg ccgatgccct gatccacgca
     2761 atctgcgatg ccctgctggg cgccgcaggc gcgggagaca tcggcaggca tttccccgac
     2821 accgatccgc agtacaaaga tatctccagc atgattctgc tgaaaaacgt caaaaaaatt
     2881 ttggacggga aaggattccg tattgagaat atcgacgcca cggttatcct ggaagttccc
     2941 agactggcgc cctatgcgga tgcgattgtc gccaatatcg ccagtgttct gaacatttcg
     3001 gaagaatgcg tgaatatcaa agcgaaaacg aacgaaggca tgggctttac cgggcgcaga
     3061 gaaggcgtgg cggtgtttgc cgttgcttcg ctggcagaaa ggatgagaaa tggtgcgaaa
     3121 acctagagtc agatttgcgc cttctccgac gggagagctt catgtcggca acgcgcggac
     3181 ggcattgttc aactggatgt ttgccaggca tcacggcggt tccttcgtcc tgcgcataga
     3241 agacaccgat gcatcgcgca gcgcgctttc gtatcaaacc aatctgtacg atgatttgaa
     3301 atggctgggt ctcgattggg atgagggacc ggaaaaggga ggacatttcg ggccttaccg
     3361 gcagagcgaa cgcgtcgcag tttacagaga attcctgcag aaattaatga aagccggcgc
     3421 ggtttatccc tgctattgca ccgaagagga gctggaggaa gaacgccaga cgctgatttt
     3481 aagcaaacgt atgccgcgat atatgggcaa gtgccgcaat ttgtcgccgg aagaaagaaa
     3541 ccgcaaggaa aaagaaggaa gaaaaccttc gtatcgcttt aaggttcagt cacaaattat
     3601 tgaatttgaa gacttaatac gcggcaatgt taaattcaat gcggaggata tgggggattt
     3661 cattattgtg cgttccaacg ggatgccggc ctataatttc gcctgcgtaa tcgatgatca
     3721 tctgatggat atgacacatg ttatccgggg cgaggatcat ctttccaata cggctttgca
     3781 gatcatgctc tatcgggctt tcggatttga ttccccggta tttgctcacc attctttaat
     3841 tctgggcagg gatcggacca agctgagcaa acggcacggt tcggtttcga tcggagaatt
     3901 ccgcaggcag ggcatattgc cggaggcgat gatcaactat ctggggcttc tgggaagttc
     3961 attcaccggc gcccgggaag tcttatcccg tgacgatatg atccaagact tctcgctgga
     4021 acgagcctcc cgaagcggcg ctgttttcga tgaagaaaag ctccactggc tcaatgcgat
     4081 ttacatccga aacacaagaa ccgacgatct ggtggagagg ctgaaaccgt ttcttgacga
     4141 accggcgtat caaaacgaaa tgcaaaaccc ggaatggttc cgaaaggctg tggaactggt
     4201 gaaaacggat ttgacaaccc ttgcagacct gaaaaatcac atcagcattt tttttgacag
     4261 tcaatacagg atttccgcag aggccaaaga cctgctggat caggaacccg cccggaaagt
     4321 ggtcgaggca tttgcctcat atcttgaaaa acaggatgcc ggaaatcaaa atctttatgc
     4381 agaggccgtc cgatttgcca gggaggcaac cggggcaaag ggcagggcgt tgttcatgcc
     4441 gctgcgcgca gcattaacag gtttgcttca cgggccggag ctggataaaa ttttcgatgt
     4501 gctggggcgg aacgcggctt taagacggct gaagtatttc gagcgctagc ggcgagcgcg
     4561 gttcttttta aaagcgataa aataaaaaat cagattgatg acaattacca ctgcaccgag
     4621 acctttctga atggacggtg tcagattttc cggatagatc aaagacagca ggtaccgggc
     4681 gataaaatca ccgccgtaaa ccgattcgcc ggcaagcgtt cgcaggtgat tttccagcgg
     4741 cgtcagcgga cagatccagc cgaaaatttc cacgaaagcc ccccagagtg ccgcggggag
     4801 atgcgcccat gcgaaggacg gtctccagat gaccagcaga ccgccgaaga caacaaaaag
     4861 aataaacaga aaatgaaccg caacaaggat gtctgccaga atatgataca cggcatccct
     4921 cgaaagatta aaagacatta tctttgggca gccagccgat gcctttggca tgcaggctct
     4981 ggatgtagag cctgtcggct gtttccgtaa caccggttgt ctccggatag cgaccgccgg
     5041 ggtcctgcag gtcggcgacg atccggccgt cttctgtaaa ggccaaaaca tggccgtagg
     5101 gtttgggaag cggccacaga aatttcggca gccggtaggt cagcttgcgc ataaaaggcg
     5161 tggaggtgat gacgcgttga tcaatggcgg gattgcgggg cttggcaaat cccagccaga
     5221 tctttccgtg aagaccgcgc atcaggttgt cgggataacc gggcaggttg tcaaacagga
     5281 tgaatgccga tttccccggg gttgcaatat ccaaatcctg tgcggttacg gaaattttcc
     5341 agacgcgata ttttcctgtt tcattgacga aaagccagtt ttcatcttcg ctcaacacca
     5401 cgccgttggc gaagctgaat cctttggcca caatccgggt ggtctgtgct gccgggtcgt
     5461 attccagaac gcgtccggtc gagcgttgtt ccagaatgtc gatcatgctg gccggaaaga
     5521 tgcctcccca ttgtttgggc gcaaaacgcg tggaggcgtc ggtgaaataa attttccccg
     5581 attgtgacac tgcgaccgca tcggcaaaac gaatggggtc gccgttgacg ttatcggtta
     5641 atacggtgat gtttttctcc gggctgatga gcaccaggcc ccggtcaacg tcggcggcaa
     5701 cgatattgcc gcgggcgtca aaatcaaaac cgagaacggg tccgccggta tcggcataga
     5761 cctcctgttg cgtcccgtcc tgattcatgc gcagaatttt tccgccggcc acggtcgcat
     5821 acagcttttg gtctttgccg atggcgatgt gttcggggcc ttcttcccgg cccagtgaaa
     5881 tcatacgcag gccggcaagt ccggtgttga cggcatgggg gcctgtatat ccgtcggacg
     5941 ggggaggaat ccactgcacg ggatcgaccg gaacaggcca gagggttaaa taagccgtca
     6001 aaagaacaac gatgagtgca accgcagcaa aaatcttttt catggcgtcc tcctgaagca
     6061 ttcaaaacat cagggcaaga ataaccggct acagcgggat attgttgtgc tttttggaaa
     6121 gccccgtttc ttttttattt cccagcgcat ccagcagagc gatgattttt tttcgggtgt
     6181 cgcgcggcgg gatgatttct tctatgatac ccatggatgc ggcaaaatag ggattgttga
     6241 actgctgttc gtaggcctca acaagttgtg cccgccgggc agtcggattg tcggccccgg
     6301 caatgtcgtt gcggtaaatg atgttgcagg ctccttcggc gcccatgacg gcaatttcgg
     6361 ccgtgggcca ggccatgacg taatcggcgc cgagctgttt gctgcacatg ccgatgtacg
     6421 cgccgccgta ggccttgcgg gtgatgacgg tgatcttcgg cacggtggct tccgaatagg
     6481 catgaagcag ttttgcgccg tgacggataa tgcccgccca ctcctgatgg gttcccggca
     6541 tgtagcccgg cacatcggta aaggtcagca aggggatgtt gaacgcgtcg cagaagcgta
     6601 tgaagcgcgc tgctttatcg gaggcgtcca cattcaggac accggcagcg aaacggggat
     6661 tgttggcgat gacgcccacc ggtctgcctg ttacccggat aaaagcggtg atgatattct
     6721 gcgcccagag ttccagcagt tcgaacagct cgccgttatc ggcgacggac tttataattt
     6781 ttttcatgtc ataggcccgg ttgcttttgt cgggaatcat cgaatccagt tccggacagg
     6841 ttcggtccgc agggtccgtg caatcggaca gaggcagggg cgcatggcaa ctgtcgggca
     6901 gatacgcaag aagctttttg attttttcaa tgcagtcggt atcgctttcg gtgacgacgt
     6961 ggcagacacc gcttttggca gcatgggcca gagcgccgcc cagttcgtca tgggaaattt
     7021 cctcgccgat gaccgccttg actacatcgg ggccggtgat atacatgaag ctgcttccct
     7081 tgaccatgaa aacccagtcc gacagagcgg gcgaatagac agcgccgccc gctgtcgggc
     7141 ccataatcgc cgtaatctgg ggaatataac cggacgccag ggtgttgcgg taaaaaattc
     7201 cgccgtagcc ttccagtgaa gggacgcctt cctgaatgcg cgcgccgccg gaatcgttga
     7261 gcgacacgaa cggtttctgc gcggcaaggg ccatctccat gaccttccag atttttttcg
     7321 catgcatttc ccccagcgaa cctccggagg ctgtaaaatc ctgcgcggca acaaatacgg
     7381 tgcggccgtt gacttttccg aagcccgtca ccacgccgtc ggcgggaata tcttttttgt
     7441 ccaggccgaa cagcgtcgat cgatgttcaa caaaaagctg cacttcctga aaggtgtcct
     7501 gatcaaaaaa taaatccagc ctttcccggg ccgtcaattt tttcagttca tgctgccggg
     7561 cgatctgttt ctcgccgccc atctgtttga gggtccgatt ctttttctca aaatgattga
     7621 ttttttcctg cgttgaattc ttctgtacat cttcgctcat acaattctcc agtctttggg
     7681 atccctacac atggaacatg aagcagtcat gttgaaaaga tattttatga ttggaggaaa
     7741 tactagcttt tttagtgaaa actggcaaga agaaatgatg aacgtatccg gcgcggcgtt
     7801 tgtgcacgta tataccgcct cagacaaaaa tctgcacttt ggcggccccc gttctgtagg
     7861 aatacgttac cgggtaatcc agacttttga tcagctgttt catgacatga ttgttggcat
     7921 caatgaggga tacaaaacag gagacgccat ggtccctgcc gatggcaaag atatccttga
     7981 gcagacgctt ccccagcccc agtccctgcc agtcgtcgcg aaccagaatg gcaaagtcgg
     8041 taacctgttc cctgacatcg taaccgtaac gcgcaacggc aatgatggca tccttgccgt
     8101 tttccggaat gaccgcaacg agggcgaaat cactgtcata gttgacatgg gtcagcttga
     8161 aaagcaggtt ctccgggatc attctgagcg gcgtcagaaa gcgccggtag atggaatcgg
     8221 gatacagttt gtcgaacagt tccagaagca gttttccgtc ggtgtcccgg atcggacgga
     8281 cgaagacgtt tttgccgttt ttcagcgcca gaaacttttc gtattgcgcg ggatatggtt
     8341 ttttaaaact catcagcata ttctatctat cctacaaaaa attttaggac agccccatgc
     8401 atgattctat ccggcgacgt ctcatttttt tgtcggagca acacccggaa gcgttgacgg
     8461 aatttgtccg acagagcacc gccggaaagg atattctgcc caagatttga ttgacagaaa
     8521 aacaggcttt tcctatactc gcaacgttgc cgttagcagg ttgaaccgtt ttgcaaaacg
     8581 cataccggga ttcaaggcag ggtgctgcgc catggttttg gccgaggcgc tgtgatgtca
     8641 ggcctgcagg tgattttgtg atccgttttt agtctggagg ttgtcatgga aaaattattt
     8701 tatcctgaat ccattgccat catcggcttg tcatcgaagc cgcaaaatat tccccgcctc
     8761 gttctggaaa acctgattcg ctggggttac aaggggcgaa tcttcgggat caatccgcgt
     8821 gccacggatt cccatgtgga cggcattaaa atgtacaaat cggtccggga attgcccgtg
     8881 gttccggatc tggtgtacgt cctcattccc gcgcgaatga ttccggatgt gatggattcc
     8941 tgcggccggg ccggcgtgaa gcggctggcg attcccaccg gcgggttcaa cgaactgggc
     9001 gaagagggca gaaagctgtc cgaactggtt ttgcagaagg ccagagaata cgggatccgg
     9061 ttcgtcggtc ccaacggtgt gacggtggcc aataccgcca acgggttgtg cctgcctttt
     9121 gttccctcgt acagcccgcc caagggagga ctctcgatca tttcgcaaag cggcggcgtg
     9181 ggcctgatgc tgtggaatct gatgacggac gaaaatgtcg gcatggccaa atttgccagc
     9241 atcggcaaca agctggatct ggatgaagtg gattttctgg aatatttcgg acgggatccg
     9301 gaaaccaaag tcatcggcat gtatctggaa agcgtnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnngtggt gttgaaagtt gtgtcaccgg atgccgttca taaatcggaa
     9481 gcgggcggcg ttatcacgga tgttcaaaat gcggaagaag ccgtcaaagc attcgatcaa
     9541 atcagaagca accttttccg ctacaacgct catgccgatt ttcagggcgt gcgggtcatg
     9601 aagcaggcaa gtgacggcta cgatatgttt atcggcggaa aattcgatga atctttcggg
     9661 ccggttgtct ttttcggtta cggcggcatc tatatcgagg ttttccagga taccgccaac
     9721 ctgctgtgtc cggcggaccg tcatgaaatc gaagccggag tccaacgatt aaaatcgtat
     9781 aatattttac aggggatgag aggaaaggga gcgggggata ttgcgggata tgtcgcgatg
     9841 attgagcggg tcagccatct gctgcatcag ttcccgcaga ttcgtgaact ggatatcaat
     9901 ccggtgcgtg ttttagccga cggatccggc gttttggcgc tcgacgcgag agtcagaatc
     9961 gacacatgat gtcagttgcc tgaaaacaat catcactcgg aatcgctatg cgcaatttta
    10021 aaataacggt ggaatacgac ggagctgcct actgcggctg gcaaaggcag cataacggga
    10081 ttgccatcca gcaactgctc gaagaggcga ttaaaaaaat caccggtcaa aacgtgtcgg
    10141 tcatcggttc cgggcgcacg gacgccggtg tgcatgccat gaaccaggtc ggaagtttca
    10201 aatgtgatac gacattgccc gttcagaaga tttttatggg cgtcaacagt gtgctgcctc
    10261 cggatattgt ggtcaaaaat ctggaagaac ggtctgacga ttttcatgct ttgcgggacg
    10321 tcaaaagtaa ggtatacgta tatcgaattt gcaatcagcg tttgagaccc gtcctcggtc
    10381 gtaattattt ctggcatgtg cgttaccctc tcgatattgc gagcatgcgg aaggctgtcc
    10441 ggctcatcat cggaacgcat gatttttcct gtttttgcgc agcagggacc gatgtcaaag
    10501 atcgtgtgcg gacaattgtc gatattgaaa ttatgaccgg agaagaaggg ctgattgaaa
    10561 ttcaagtgga gtctcacgga tttctgaagt acatggtgcg aaacattatc ggcacgctga
    10621 tagaggtcgg acgcggtaaa cgacaacctg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nggtgcgaaa cggacaggga aacatgtttt gccgtcaacg ccgaagcggt
    10801 caaaaatatt gccggggcct gccgtcaaaa aaaaatccgc gtgctgcatt tcagcacgga
    10861 ttatgttttt gacggaaccg cccgaaaacc gtacagggaa gatgctttgt gcaatcccgt
    10921 caatctctac ggagcatcga aactggccgg tgagaattat ctgcggacgt tgtcggacaa
    10981 ttaccttttg atccgctcct cgtggttata cggaatcaac gggaaaaatt ttgttcaagc
    11041 cattctggat aaggccaagg caaaagaatt catccgcgat accatgaaca gggccaaagc
    11101 cgttgccgaa gctccctcaa cgctctctgt ggtcgatgat caggtcggtt cacccaccta
    11161 tacaaaagat ctggcactgg ccgttgcttt cctcatccgg aaaaacgcaa ccggtatcgt
    11221 ccatgtgacc aacagaggaa gctgcagctg gcatcagttt gccgtcagga ttttaaaaag
    11281 cgcgggatat ggtcatatcg ttgtggaacc gataaagtct tccgaactgc aaagaccggc
    11341 catgcgcccg gcgtacagta ttttaagcaa caagacgttt attgaaatta cgggcaaggc
    11401 catgcagccg tggcaactcg ctttagagga ttacctgaac aattttaaat tgaaaaaaag
    11461 tcattcatga aatcacgctc cgacgtgttt tctctccttt ggttcatagg tcggaacaaa
    11521 aaagtaagat cgttttgatg agttgaccga aacccggcag gtctgtgtaa aaatgaccgg
    11581 gaaggcggtt ggcatcgttg caggcgaggc tgttcgaaga tttgccggaa aggccattga
    11641 accatatccg gttacacaaa agacatcgaa cgaaaatcat caaaaaggtc tgggccggtg
    11701 atcgtttaga aaatttattg tttagatcaa aaacaggctt gacgacagac gggtgccctt
    11761 tatgaaaaag gcgatgcttg ttcatttaaa cgaactggat ttttatttaa agaaaatcga
    11821 tgccgtttgg cctgttcgtt gcctcaccgg agaggcgata gattgtgaaa aggtcgtcga
    11881 ttattaccgg caaagccgga tcgtttacaa gtacgtgcat tcctacagcg gcgcgatgca
    11941 tatggccgtc aattccgacg gtgtcttcaa tgcaaagggt ttttacacgt ccctcaacga
    12001 aattgtcgag atcatcagtc cgttgccggc cggcgccgtt ctggaactgg gatgcggcaa
    12061 gggattcaat tcggcttatc tggcaaaaaa aataccacac gctcatttta ccggaatcga
    12121 tgtcagcggg gagcatctgg cgattgctcg aaaaaaagca tcccgcatac acaacctctc
    12181 cattctgtac ggagactttc aagatatcat gtttccggac ggtgcttttg atacggtctt
    12241 tgaactggaa tccatctgtc atgcccgaaa agtgcggcag gtattatccg aagttttccg
    12301 ggtgctgaaa aaaggagggc gatttattgt ttacgacgga ttccggcaaa gcggtttcca
    12361 ccgtttgccg gatcgtttaa tcgaggcggc ccggatgact gaaaaggcac tggccgtcga
    12421 tcacttcgaa gatattgacg gctggctggc catggcggtt gcggaaggat tccgcatcca
    12481 tgagaaaaaa gatttgtccg cgtccatcat gcccaatctt gcccgacttc aatatctggc
    12541 gcggcgatat tttgaatacc cgatgctgac gaaactgctg ctgcgtcttt tgaatgtata
    12601 taccatgagg aactgcatcg ccgttcttct gatgccgttt acccttcaca acaacgcgca
    12661 tggctattac aaaataatac ttgaaaaatg agtccgttgt ttctatggta tccaaaacgg
    12721 aaagattttt actcattgcc cgggggataa cgcatgcaag tcaatctcac tgaacaggaa
    12781 attgtca
//
LOCUS       DAONQZ010000065        12578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5538_length_12578_cov_14.956241, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000065 DAONQZ010000000
VERSION     DAONQZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5538_length_12578_cov_14.956241"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1337
                     /locus_tag="PLR13_09370"
     CDS             105..1337
                     /locus_tag="PLR13_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19590.1"
                     /translation="MILKKEQRSYLEENLGKTILPLSIFENEMRPGTEPLEVLIFYKK
                     KTPIQKLKDSFFKAAEHYNLFSSRLIMIDDNKYALHHCKEGINYHLLPPLDVTFDHAN
                     IDEIRKMIVPVETLPGEPMVALTGIPIKDGVFGGISCSHAMTDGISLLLFLFAWSCII
                     EEKEFLPPSPQRLFKGEPVRFDEIDKAFIPGLSELSDKIQNRVKYRKNVTVYSKKEYF
                     SDGFLEEVKKKAKKENDKYFISNNQIINSFLLKKYHKNILPDTDSVRVRIPINFRGIH
                     PEIDPFYIGTAYFIDIVEFSKTEIDKLSMYEIAYRLHESITSSRNVDYIKKISYLSDY
                     GIEIKTDMLKNFSPYDWKRDIVSSNLTHLNDLESMGLGSNVGSIVHIGVTAPSSFAMM
                     KEKNGSIFAQITSQYPLP"
     gene            1525..2766
                     /locus_tag="PLR13_09375"
     CDS             1525..2766
                     /locus_tag="PLR13_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19591.1"
                     /translation="MKNRTTANGTIKTYLKSHLGKNILPLSILDNEIRPGGNPIEILL
                     FYKREVSVLNIQDSFRKAVEHYNLFSSRLIMMDHDQFALQYCSDGAVINLLPAVNETH
                     DSIDIELIRKMMTHVKTLPGEPLFALTVVPVRDGYLGGFSFSHAIGDGVSLILFLFSL
                     GLIMAGEHFPLPSSQRLFTGRPLSVDQIDKSFILPLHALNKKIQDRIRQANVQTYTTR
                     EYFSEEQLKAIKHQAKSAGENHDISNNQIMTSLLLKKYHHNLLPDTDRIRLRSPVNFR
                     EIHPDIDPCYIGNALFVSFTEFEKDEIDRMPVYEIACRFKKSIMQMRNENFVKKISYL
                     SEYGIEFQYEALEKYLPHNTDTDIVSTNLTHINDLESLGLGSTMGSILYIGLPLQPCF
                     TMLKEKSGSIFAQITGRYPFT"
     gene            2841..3216
                     /gene="rnpB"
                     /locus_tag="PLR13_09380"
     ncRNA           2841..3216
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLR13_09380"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3248..3502)
                     /locus_tag="PLR13_09385"
     CDS             complement(3248..3502)
                     /locus_tag="PLR13_09385"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQC19592.1"
                     /translation="MKGYLIGKKPKKDREPISKFSGILMLSAWGFAMVLSSFLFLYVG
                     YIIDEILGTTPNFMLCSFILAIGLCIWTLYKDAVKKGKDL"
     gene            complement(3563..3763)
                     /locus_tag="PLR13_09390"
     CDS             complement(3563..3763)
                     /locus_tag="PLR13_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19593.1"
                     /translation="MRSFPFIHILVLIISGFVLFILKKKYRQISTSELIMIFILVFAL
                     VAIFTDTGIDLIKSLIAVIQVD"
     gene            complement(3950..5260)
                     /locus_tag="PLR13_09395"
     CDS             complement(3950..5260)
                     /locus_tag="PLR13_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQC19594.1"
                     /translation="MSETKKEKRFFNIGGVFFLLIVIPLSLMAFLIANGMFKLGVTVK
                     EKTVNVLDQKSQEDIKARAVNTANEIAKFLMDTKKDLQVATILPSTEAVFKQFVSENK
                     KPVWVKRDGKVQQILIPLYSEMTLIDKNGNEQIKIVDGQAVPADKRVNVSNPANTVYK
                     TEDYFAKAKNLNKGEIYVSPATGYYVDKAAFEKGKRFEGYVRFATPIFSKDGFAGVIT
                     LALDYRHLAEFTDQLVPTQAEKVFETDAATGNYAYMVDNRGFVISHPADYHIVGLTQQ
                     GTTVPTLSADNAKELAKKGAEALNMFQLGFMDHNIFNIAKEAIVGKSGILMYKYGGLT
                     KFVAYAPIKFYASNLPEPAGFGWVGLGLDVEKYNQEAAKVAKEIEKEAKAWTATVIFI
                     LIASMIILFFIMLLLVRGITRSLQAEVPKGSEGPLSYDDDDDEK"
     gene            complement(5308..5685)
                     /gene="atpE"
                     /locus_tag="PLR13_09400"
     CDS             complement(5308..5685)
                     /gene="atpE"
                     /locus_tag="PLR13_09400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQC19595.1"
                     /translation="MSHKLGKVILTAFTCMLVLLVASPFVFAAEAIPAGGESYIKVYF
                     AVGAMIGAGLAMGLGAIGAGAGIGNAANGACQAVGRNPGVQGKIMMVMLVGMAMAESI
                     AIYALVISLILLYANPYMRYFLG"
     gene            6383..8995
                     /locus_tag="PLR13_09405"
     CDS             6383..8995
                     /locus_tag="PLR13_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19596.1"
                     /translation="MTLFSKFKILLYKAAQLRILFASVTVVILFLLLALTVNNSREKD
                     IVNIFSRQQLVNVQNTSKRMMDVFSQIGKNIDLFSRLHPDLTIDEKELDSYYKMLSSG
                     WEDTFDAIVMFDAGGNIKNVYPKNVVPSINVSEHFNVLSKQQKRYLGLALPDASGTLG
                     SKQKHDRYLVLGYPLRKPNGEFSGALLISFSLSAVLHKYRQRPADDELGELWLIDEQQ
                     KILVHPRWAFVGNDIKKFLKKSDEQASMFSSENGDNFEAVVLQGDKIKQRSIISYYPV
                     KAGDKKWTMLVVAPYSKVISPLRKTFFYTLFSSLLLIIIVIATSMSFAYKEGKRLRHR
                     EERKRLKERRDWQEKLLREKKTIEGIIEGSPIPSFVINNKHKVILWNRACSELTGHSA
                     DEMLGTGNHFKPFYSVTRPMIADLIVDSDMDGLSKHYGTKNVKKSEKVVGAYEATDYY
                     ANLGGQSRYLYFLAAPIYDEEGKIIAAVETLQDVSREREMTKSLSEYAETLQNELIEN
                     IELQRQIQDLNNYLQTIITSLPDRIYEIDEKGIINFMSRGLRKKTGTSGEFKNKYFLD
                     FVASGYEDLVLSKWEEAKKGIYKPYEIEAIGKDGCKHNLLITTTPVIGTDHYIIVQRD
                     ITEFKDLERKLYDSQKLAALGQLSAGIAHEIRNPLSSIKMSLQILTKRMKPDGNDLKR
                     FRIAEKEVEHLETLVNDILAFAKPVDPRKTSVDLSKVLEQAIALSEKGITDKKIEIVT
                     QFGKVFPVTVDAAMMTDAFINVIRNAVEALPENGKIVVSLKYADETRHSAAVEITDNG
                     SGIDETDMPHIFNPFFTRKNYGTGLGLSLVKKIIDIHRGTIDISSRKNEGTKVLIVLP
                     LRLDQAFSPAANNE"
     gene            9008..10390
                     /locus_tag="PLR13_09410"
     CDS             9008..10390
                     /locus_tag="PLR13_09410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC19597.1"
                     /translation="MPSILVIDDDASIAETLDLYLTEEGYNVRTALTGTEGLNQYVQE
                     PSDVVILDIRLPDIDGFTVLEDLKEENENVKVIMITAFHDMETTIQAMKSGAFDYIHK
                     PVNVDELDLAIKKALKSLEMEKKIDGLLMEPSRSFRVGDIIGTGNEMREIFKIIGTVS
                     QSRTTVLIQGESGTGKELIAKVVHNNTSPDEPFIAVNCSAIVETLLESELFGHEKGSF
                     TGAVARKLGKFELARFGSVFLDEISEMSVNLQAKLLRVLQEMEFDRVGGKEKVKVNAR
                     IMAATNKDLKALVKEGKFRDDLYYRLNIVSITLPPLRARRQDIPPLIDYLLTKINLDL
                     HKKIIGVSDEVMEIFMQYTWPGNVRELENLLVRACVVAKGQVLGVNDFPELGKEEAAG
                     PAGDRDANLFQASEPGKLLTLDEVEERYIRKVIKETDKNKGEICEILGVSRPTLERKL
                     EKYGITFERE"
     gene            10734..11627
                     /locus_tag="PLR13_09415"
     CDS             10734..11627
                     /locus_tag="PLR13_09415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19598.1"
                     /translation="MISVIIPTFNRGYTLAQVLDSYYRQQFVAELIIVDDGGSDKTAV
                     VVDEFVRKYPRIKTMYLKNEKRQGAPYSRRRGLLAADGEFVLFGDDDDFLEENYTTVC
                     LNKLKSLSADIVSGRHFFRTPGETVAEAIARFGQGSIDVPPFDFRLFYINTDAKFKSD
                     VELPFTHAIYLVRRELLLSYEMDTFYSRGNGFREENDIQAKIHINGGKIIITNDTHSV
                     HMHSSEVQTGGQRVGALAKLFWSLYYTAYFFKKYYKPIMERQGENGCYLKGIVIYYAL
                     TLLTFPFLVLKEKRILRSMRK"
     gene            11651..12268
                     /gene="yihA"
                     /locus_tag="PLR13_09420"
     CDS             11651..12268
                     /gene="yihA"
                     /locus_tag="PLR13_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQC19599.1"
                     /translation="MRQIIMKITSAQFIKSAVWPPQYPPAIMPEIAFVGRSNVGKSSL
                     MNTLVGRKNLAKTSNTPGRTQLINFFTVNETISFVDLPGYGFAKVPRSVKKDWGDMME
                     AYLRERQSLCLVIFILDIRRDPNADDLSLRDWLDHYRIPYLYILTKSDKLSNNQAIAR
                     KRAIEKMLRISSEKKTILFSAKTQKGKVEILEFLENHLRALDKHS"
BASE COUNT         3647 a         2629 c         2852 g         3450 t
ORIGIN      
        1 cacattattt taggaaagtg ttaaaatcat tgcaaaaata tttcttaaaa tttgttgtcg
       61 tttttgaaga caattgcaat aaatttattt ttggagatat ttaagtgata cttaagaaag
      121 agcaacgtag ctatttggaa gaaaatcttg gcaaaacgat tctgccgttg tccatttttg
      181 aaaatgaaat gagaccggga acggaaccgt tggaggtatt gatcttctat aagaaaaaaa
      241 ctccaataca aaagctcaaa gacagttttt tcaaggctgc cgaacattac aatctttttt
      301 ccagtcgtct catcatgatt gatgacaata agtatgcgtt acatcactgc aaagaaggta
      361 ttaattatca tcttttaccg cccctcgatg taactttcga tcatgcaaat attgatgaga
      421 tcagaaaaat gattgtgcct gtcgaaacat tgcccggaga acctatggtg gcgcttaccg
      481 gaataccgat caaagacggc gtattcggtg gaatcagctg ttctcatgca atgactgacg
      541 gtatctcgtt gttgcttttt ctgtttgcct ggagttgcat catagaagaa aaggagtttc
      601 tgcctccgtc tcctcaaaga ttattcaaag gtgaaccggt tcgcttcgat gaaattgata
      661 aagcctttat tcccggcctg tccgaattga gtgacaaaat tcaaaaccgg gttaagtata
      721 gaaaaaatgt aaccgtatat tctaaaaaag aatatttttc ggatggcttc ctcgaagaag
      781 ttaagaagaa ggctaaaaaa gaaaatgaca aatatttcat ttccaataat caaattatta
      841 attcattctt attgaagaag tatcataaga acatcttgcc cgataccgat agcgttaggg
      901 ttcgaatccc catcaatttc agagggatac atcctgaaat agatccattc tatatcggta
      961 cggcatattt tattgatatt gttgaattta gcaaaacaga gattgataaa ttgtcgatgt
     1021 atgaaatcgc ttatcgtttg catgaatcaa ttaccagctc aagaaatgta gactacatta
     1081 aaaagatatc atatttatct gattatggga tcgagattaa aacggatatg ttgaaaaatt
     1141 tttctcccta tgactggaag agagacatcg tttcttccaa tttaactcat ttaaatgacc
     1201 tcgaatccat ggggctgggc tcgaatgtcg ggagtattgt acatattgga gttacagccc
     1261 cctccagttt tgccatgatg aaggaaaaaa acggaagtat atttgcacag ataacgagcc
     1321 agtacccatt gccataatca taaatatgat cggacatcta tttcttgatg aatcagatca
     1381 ataacattta ggagacatcg tgcctttgca tggacgttct gtatttctgg tcgcatcgtt
     1441 cctgcaggta aattttgtat tccttatgaa ggacataact tcatctaaat ttaatacaaa
     1501 tattattcaa ttgcagaaac gataatgaaa aacagaacaa ccgctaatgg aaccataaag
     1561 acgtatttga aaagtcatct cggaaaaaat attcttcccc tgtccatcct ggacaatgaa
     1621 atcagaccgg gagggaatcc catagaaatc ctgttatttt ataaacgaga ggtttctgtt
     1681 ctgaacatcc aggatagttt caggaaggcc gttgagcatt ataacctttt ttccagccgc
     1741 ctgatcatga tggatcacga ccagtttgca ttacagtatt gttccgatgg cgcggtcatt
     1801 aaccttttgc cggcggtgaa cgaaacgcat gacagcattg atattgagct tatcagaaaa
     1861 atgatgacgc acgtcaaaac cttacccggg gagccgcttt ttgctttaac ggttgttccc
     1921 gtcagggacg gatatttagg cggcttcagt ttttcgcacg ccataggcga tggggtatcg
     1981 ttgatccttt ttctgttttc actggggtta atcatggctg gcgagcattt ccctctgccg
     2041 tcgagtcaga ggctttttac gggcaggccg ctcagtgttg atcaaataga taaatcgttt
     2101 attctgccgt tgcacgcctt gaataagaaa attcaagata gaatcaggca ggcaaatgtg
     2161 caaacataca caacgagaga atatttttcc gaagaacagc ttaaggccat caagcatcag
     2221 gcaaaatcgg caggcgagaa ccatgacatt tctaataatc aaattatgac atcgcttttg
     2281 ttgaaaaagt atcatcataa tctgctgccc gatacggaca ggattagatt aagaagcccg
     2341 gttaatttca gagagatcca tccggatatt gacccgtgct atatcggcaa tgcgttgttt
     2401 gtgagcttca ccgaatttga gaaagatgaa atagaccgca tgccggtgta tgagattgct
     2461 tgtcgattta agaaatcgat tatgcagatg agaaatgaaa atttcgtaaa aaaaatatca
     2521 tacctgtcgg aatacggcat cgaatttcag tatgaggccc tggaaaagta tctgcctcat
     2581 aatacggata ccgacatcgt ttcaaccaat ttaacgcata tcaacgacct ggaatccctc
     2641 ggtttgggct cgaccatggg gagtattctg tacatcggtt tgccgcttca gccgtgtttt
     2701 acaatgttaa aagaaaaaag cggtagcata tttgctcaga ttacaggcag atatcccttc
     2761 acgtgaaaag gcctattgaa gatttttcca aaaggggtat tgaaagaaac ggcaatccgg
     2821 tataacatca ttaaaaggct ggagaagatc agatgatcgc cggtttgctt tgggcagccg
     2881 gaggaaagtc cgagctccgc agggcaggat ggtcgttaac ggcgaccggg ggcgacccca
     2941 gggaaagtgc cacagaaaag ataccgcccg ggcgaaagcc ttggtaaggg tgaaaaggcg
     3001 aggtaagagc tcaccgcttt cccggcgacg gaaaaggcag ggtaaacccc atccggagca
     3061 agaccaaata ggagaacgtt tgagggcggc ccgcctgatg ttctcgggta gtgtcgctag
     3121 aggcgtcagg caactgacgt cccagatgaa tgatcatcga cgtgttgagg gtaaccgata
     3181 cacgaaacag aactcggctt atgatcttct ccccgccttt ttgccgtaac gatttttcga
     3241 aaggcgatta taagtctttc ccttttttga ccgcatcctt gtaaagcgtc cagatacata
     3301 atccgatagc gagaataaag gaacaaagca taaaatttgg tgttgttccc aggatttcgt
     3361 cgatgatgta tccgacatag agaaacagga aggaagaaag taccatggca aagccccagg
     3421 ccgacagcat caaaatgccg ctgaatttac taatcggttc tcgatctttc ttgggtttct
     3481 ttcctatcag gtatcccttc atgcttctcc atccacttca tgatcttcat tgataagatg
     3541 gccggaatga tacagacgcg ggttaatcca cctgaatcac tgcgataagg ctttttatca
     3601 gatcgattcc ggtgtctgta aagatggcca ccaaggcaaa aacaagaata aatatcatga
     3661 tcagttcgct tgtactgatt tgccggtatt tttttttcag aatgaaaagc acaaagccgc
     3721 tgataatcaa aaccaggata tggataaaag gaaaacttct catgttgata tcctcatttg
     3781 gcgtgacgtt aatttaataa aatgcagatc cttgtccatg aatcggttac gttgttggca
     3841 ttatatatga tgtccggtaa agttcacatg tttttttacg atttcgcgat aaaaaaaggc
     3901 cgaatcgaaa ttcggccttt tggttaatga ttgcacaagt tgattttaat tatttttcat
     3961 cgtcatcatc gtcatacgat aaaggacctt ccgagccttt cggaacctcc gcctgtaagg
     4021 agcgggtaat tccgcgaacc aggagaagca tgatgaagaa cagaataatc atggaagcga
     4081 tcaggataaa gatgaccgtt gccgtccagg ctttggcttc tttttcaatt tccttggcga
     4141 ccttggcagc ttcctggtta tacttttcaa catccagccc cagtccgacc catccgaatc
     4201 cggccggttc gggcagattg gatgcgtaga atttgatagg tgcataagcg acaaacttcg
     4261 tcagtccgcc gtatttgtac atcagaattc ccgatttccc tacaatagct tccttggcga
     4321 tattgaagat gttgtgatcc ataaacccaa gctggaacat gttcaacgct tccgcaccct
     4381 tcttggccag ttctttggca ttatcagccg aaagcgtcgg gaccgttgtg ccctgttggg
     4441 tgagtccgac aatgtgataa tccgccggat gagaaatgac gaatccgcga ttatcgacca
     4501 tatacgcata attacccgtt gccgcgtctg tttcgaatac cttttcggcc tgtgtgggaa
     4561 caagctgatc cgtaaattcg gccagatgac gataatccaa tgccagtgta ataacgccgg
     4621 caaacccgtc tttactgaat atcggtgttg caaaacggac gtatccttca aaccgttttc
     4681 ccttttcaaa cgctgccttg tcgacataat agccggttgc gggggaaaca taaatttcac
     4741 ctttattcag attttttgcc ttggcaaaat aatcttccgt tttatatacc gtattggcag
     4801 gattgcttac attaacacgt ttgtctgcgg gcaccgcctg accgtcaacg attttgatct
     4861 gttcgttgcc gtttttgtca atcagagtca tttcgctgta gagcgggatc aatatctgct
     4921 gaactttgcc gtcgcgtttg acccacaccg gttttttgtt ttcagaaaca aactgtttga
     4981 atacagcttc tgtcgaggga agaatcgttg caacttgtaa atcttttttg gtatccatta
     5041 ggaatttggc aatttcattt gccgtgttga cggctcttgc ttttatatcc tcctgggatt
     5101 tctgatcgag aacgtttacc gtcttctctt ttacggtaac ccccagttta aacatgccgt
     5161 tagcgattaa aaacgccatt agtgataagg ggattacgat aagcaggaag aacacgcctc
     5221 ctatattgaa aaaccgtttt tcttttttag tttctgacat tactatttcc ccctaacctt
     5281 tgttattttg tagttaattg ttttatatta acccaaaaaa taacgcatgt aagggttagc
     5341 atagagaaga atcagagaaa taaccaaagc gtaaatggcg atggattccg ccatggccat
     5401 accgacgagc ataaccatca tgatcttgcc ctgaacaccg gggtttcttc ctactgcctg
     5461 acatgcgccg tttgcagcgt taccgatacc tgcgcctgcg ccgattgcgc ccagtcccat
     5521 tgccagaccg gctccaatca ttgcgcccac agcaaaatac actttaatgt aagattctcc
     5581 acctgccgga atagcttctg ccgcaaatac gaatggactt gctaccagca gaaccaacat
     5641 gcatgtaaat gctgttaaaa ttactttacc caatttgtgt gacattgttt gtctcctttc
     5701 ataataaaat gtttattatt acttcttttt tccataacat aaattaacat tcgatggaaa
     5761 gcgaaataat tttacctctt gctgcggcaa gattactgca tgagttgtgc catatctttg
     5821 atatgaaaat atttttcaaa gatgaaaaag gaggtcacaa ggaagaggca tatctaatgt
     5881 attgagatta tttcatttga cgcgcttaac cgacataaaa aagatatccg aaaaatatat
     5941 ccaaaatgtt ttttgatcat tttaaaagca ttcctttgcc gcgacgttta atgttttgtt
     6001 aaatacggct ataaaaaaac attcaagtgg ctttcggcag aatacttccg tatacggggg
     6061 ttgatttttt tctcttgctg ctagtggtca cagcgcactg atgaataagg aaacgttctt
     6121 tcttatattt ttattgaaat tttcgtccaa ggccgttact atcgtcaaaa taaaattaag
     6181 agacgcgtta taaaggatga ttatgcagga gcttttaacg gcaataactt accctatcgt
     6241 atggcttatc tgttcgtatg ttttaagaat tgaaagcggc ttggccaaac ttcttatttc
     6301 attaacggcg gcaattctgg tttatgtcct gatcggaata aagctttctc ataaagaaaa
     6361 caaagaggat taaaaagatt aggtgacttt attcagcaaa tttaaaatcc ttctttataa
     6421 agctgcacaa ttacgcatat tgtttgccag tgttaccgtc gtgattcttt ttctgcttct
     6481 ggctctgacg gtaaacaatt cccgcgaaaa agacattgtc aatattttca gccggcagca
     6541 gcttgtcaat gttcaaaaca cctccaagag aatgatggat gttttttccc agatcggtaa
     6601 aaatattgat ttattctccc gcctccatcc cgatttgaca attgatgaaa aggaactgga
     6661 tagttattat aaaatgttgt cttccgggtg ggaagacact tttgatgcga ttgtcatgtt
     6721 tgatgccggc ggaaacatca aaaacgttta tccgaaaaac gttgtgccgt cgatcaatgt
     6781 atccgagcat tttaatgtgc tcagtaaaca gcaaaagcgt tatctgggcc tggctctgcc
     6841 ggatgcttcc gggacgctcg gttcaaagca gaaacacgac agatatctgg ttttggggta
     6901 tcccctccgg aagccaaatg gcgaattcag cggggcgttg ctgatttcat tttctctttc
     6961 cgcagtttta cataaatacc ggcaacgacc cgccgacgat gaactgggag aattgtggct
     7021 gatcgatgaa cagcaaaaaa tactggttca tcctcgttgg gcttttgtcg gaaacgatat
     7081 aaaaaagttt cttaaaaaat cggacgaaca ggcatcgatg ttttcatccg agaacggcga
     7141 taactttgaa gcggttgtcc tccagggtga taaaataaaa caacggagca tcatctccta
     7201 ttatcccgtt aaggccggcg ataagaaatg gacgatgctg gttgttgctc cctacagcaa
     7261 agtcatctcc ccgttgagaa aaactttttt ctacaccctt ttcagctccc tgctgctcat
     7321 catcatcgtg atagcgacca gcatgtcttt tgcctataag gaaggaaaaa gactgcgtca
     7381 ccgggaagaa agaaaacggc tcaaagagcg cagagactgg caggaaaaac tgcttcggga
     7441 aaaaaagacg attgagggca tcatcgaagg atcgcccatt cccagctttg tcatcaataa
     7501 taagcacaaa gtcatcttgt ggaatcgtgc ctgttccgaa ctgacaggcc attccgccga
     7561 tgaaatgctg ggaaccggaa atcacttcaa gcccttttat tccgtgacac gtcccatgat
     7621 tgccgatttg atcgtcgaca gcgatatgga cgggctcagc aaacattacg gaacaaaaaa
     7681 cgtcaaaaaa tccgaaaagg ttgtaggcgc ctacgaggcg acggattatt atgccaatct
     7741 cggcgggcaa agccgttacc tgtattttct ggcggctccc atctacgatg aagaaggcaa
     7801 aatcattgca gcggttgaaa cattgcagga tgtgtcgcgt gaaagagaga tgacgaagag
     7861 cctcagcgaa tatgccgaaa cgctgcagaa cgaattgatt gagaatattg aactccagcg
     7921 acagattcaa gatctgaata attatctgca aaccatcatt accagtctgc ctgacagaat
     7981 ctatgaaatc gatgaaaaag gcatcatcaa ttttatgagc cggggcctga gaaaaaagac
     8041 aggcacatcg ggtgaattca aaaacaaata ttttttggac tttgttgctt ccggttatga
     8101 agatctggtt ttgtccaaat gggaagaggc caaaaaaggc atttataaac cgtatgaaat
     8161 cgaagcgatc gggaaagacg gctgcaaaca taatctcctg attacgacta cgccggtcat
     8221 cggcaccgat cattatatca ttgttcagcg cgacataacc gaattcaagg atctggaaag
     8281 aaaactttac gacagccaga agctcgcggc cctgggacaa ctttccgccg gcatcgcgca
     8341 cgaaatacgc aaccctcttt cctccattaa aatgagtttg cagatcctga cgaaacgaat
     8401 gaaaccggac ggaaatgatt tgaagcgatt caggattgcg gaaaaggaag tggagcatct
     8461 ggagacgctg gtcaatgata tcttggcctt tgccaaaccg gtggaccccc gaaaaacgtc
     8521 tgtggatctg tccaaggttc tggaacaagc cattgcgtta tccgaaaagg gtattaccga
     8581 taaaaaaatt gaaatcgtca cgcagttcgg gaaagttttc ccggtgactg tcgatgcggc
     8641 gatgatgacg gatgctttca tcaacgtgat tcgcaatgcc gtggaagcgt tgccggaaaa
     8701 cggcaaaatc gttgtgtcgt taaagtatgc cgatgaaacg cgccattcgg cggctgtgga
     8761 gatcacggat aacggcagcg gtattgatga aaccgatatg ccgcacatct tcaatccttt
     8821 ctttacccga aaaaattatg gtaccggttt ggggctttct ctggtaaaga aaataattga
     8881 tattcatcgc gggacgattg atatttcaag cagaaaaaat gaaggaacaa aggtgctgat
     8941 cgttttaccc ttaaggctgg atcaggcttt ttctcctgcg gccaataacg aataaggtga
     9001 ctgtattatg ccgagtattc ttgtcattga cgacgatgct tccattgccg agaccctgga
     9061 tctctatctg acggaggaag gatataacgt ccgcacggcg ttgacgggga cggaaggtct
     9121 gaatcaatat gttcaggaac cttccgatgt ggttatcctg gatatccgcc tgccggatat
     9181 tgacggattc accgttctgg aagaccttaa ggaagagaat gaaaatgtaa aggtcattat
     9241 gattaccgct tttcacgaca tggagacaac catccaggcg atgaaaagcg gcgctttcga
     9301 ttatattcat aaacctgtca acgtcgacga actggatctg gcgattaaaa aagccctgaa
     9361 atccctggaa atggaaaaga agatagacgg gcttctgatg gaaccatccc gcagctttcg
     9421 tgtgggcgat atcatcggca ccggcaacga gatgcgggaa atatttaaaa tcatcggcac
     9481 ggtttcccag agccgcacca ccgtactgat tcagggggaa agcgggaccg gaaaagaatt
     9541 aatcgccaaa gtcgttcata acaatacgtc gcccgacgaa ccttttattg ccgtgaactg
     9601 ttccgctatt gttgaaacgc ttctggaatc ggaattgttc ggtcatgaaa aaggatcgtt
     9661 taccggcgcc gtggcccgca aattgggcaa attcgaactc gcccgttttg gttcggtctt
     9721 tctcgatgag atcagcgaga tgtccgtgaa cctgcaggcg aaacttttgc gtgttctgca
     9781 ggaaatggaa tttgacagag tcgggggaaa ggaaaaagtc aaggtcaacg cgcggattat
     9841 ggccgcgacc aacaaggatt tgaaggcgct ggttaaggaa ggcaaattcc gcgatgattt
     9901 gtattaccgg ttgaacatcg tttccatcac gctgccgccg ctgcgcgccc gccgtcagga
     9961 tattccgccc ctgatcgatt acctgctgac caaaattaat ctggatcttc acaagaaaat
    10021 catcggtgtt tccgatgaag tgatggaaat atttatgcaa tacacctggc cggggaatgt
    10081 ccgtgaactg gaaaatctgc ttgtccgcgc ctgcgttgtc gccaagggcc aggtgctggg
    10141 agtcaacgat ttcccggaat tgggcaagga agaagctgcc gggccggccg gtgaccgtga
    10201 cgcgaatttg tttcaagcgt ccgagccggg caagttgctt acactcgacg aagtggaaga
    10261 acgctatatt cgcaaagtta tcaaagagac ggataagaat aaaggtgaga tttgcgaaat
    10321 cctgggggtc tcccgtccga cgctggaacg gaaactggaa aaatacggca tcacttttga
    10381 acgggaatga tgggctgttg aaaaatgcat acctcctaaa gggcgcggtg gtctgcttta
    10441 ttgcgctgta ctcatatcgt agcgacatgc gacaaaagca ggacttaatc cgcgatacct
    10501 gagcattagc atcccggcgc ttttaaacag cccataaaac tcatcggtcg cctccggatt
    10561 tcctttctga acgaagtaga gataaaaccc gtatgatcga tctcccgaga tttaactgtg
    10621 tttgataata aataatgaac gtgcggtgtc gtaagtattt aggatacaga gtcttgacct
    10681 gtacctattg atgaaatgag atgacaaata acgggaaggg tactgagact tttatgatca
    10741 gcgttatcat tcccaccttc aaccgggggt acacccttgc tcaggttctg gattcgtatt
    10801 accggcagca gtttgttgca gagctgatca ttgttgatga cggaggctcg gacaaaacgg
    10861 ctgtcgtggt tgatgaattc gtccggaaat atcctcgaat aaaaacaatg tatctgaaaa
    10921 atgaaaaaag gcagggcgct ccctattcac gccggcgggg acttctggca gctgacggcg
    10981 agtttgttct tttcggtgat gacgacgatt ttctggaaga aaattacacg acagtttgtc
    11041 ttaataaact gaaaagtctg tcggcggata ttgtttcggg acgccatttt ttccgcactc
    11101 cgggcgagac tgtggctgaa gcgattgccc gtttcggaca ggggagtatt gacgtgccgc
    11161 cttttgattt ccgccttttt tatatcaata ccgatgcgaa gtttaaaagc gatgtagaac
    11221 ttccttttac ccacgccatt tatctggtca gaagagagct tttattatcg tacgagatgg
    11281 atacttttta cagccggggc aacggattcc gtgaggaaaa cgacattcag gcgaaaatac
    11341 acataaacgg cggaaagatt attataacca atgatacgca cagtgtgcat atgcactctt
    11401 cggaagttca aaccggtggg caacgcgtcg gagctcttgc taaacttttc tggtcgttgt
    11461 attacacagc atattttttt aagaaatatt acaagccgat tatggaacga cagggggaaa
    11521 atggatgtta tttaaaggga atcgttatat attacgcttt aacgctgttg acctttcctt
    11581 ttttagtctt gaaagaaaag agaatcttaa ggtcaatgag aaaatagaac gcatagatta
    11641 agacaaggta ttgcgtcaga taattatgaa aatcaccagt gctcaattta taaaaagtgc
    11701 cgtgtggccg ccgcagtatc ctccggcaat catgccggag atcgcatttg tcggccgttc
    11761 caatgtcggt aaatcgtcgc tgatgaacac gctcgtcggc cgcaaaaatc tggcgaaaac
    11821 cagtaacacg ccgggacgga cgcagttaat caattttttt accgtcaacg aaacaatatc
    11881 ttttgtggat ttgcccggct acggttttgc caaagttccc cggtccgtca aaaaagactg
    11941 gggggatatg atggaggcct atcttcggga gaggcaatct ctttgtctcg tgattttcat
    12001 tctggatatc cggcgggatc ccaatgccga tgatttatcg ttgcgcgact ggctggatca
    12061 ttaccgcatt ccttatttat atattctcac caagtccgac aaattatcca acaaccaggc
    12121 gatagcgcgc aaacgcgcga tagaaaaaat gttgcggata tcaagtgaaa aaaagacaat
    12181 tttgttttcg gcaaagacac aaaaagggaa agttgagatc ctcgaatttc tggaaaatca
    12241 tttaagggct cttgacaaac attcatgacc aagaaatact atttattttt gaatgccggt
    12301 cagtgaacgc cggtcatttt tttagtcttt ttatatcaat gagttagcat aaaaaatcaa
    12361 ataaattcag cttgtgctct tgacagtgta ttgaatttgt gttagcgaaa aaacgcctag
    12421 cggcgcggca ttaaaaaagt tttttaagtt ctttaataca gaataaatga ggtgtaatca
    12481 ggtgttgaaa gataacgaaa aagttggtgc tgtaatggta gtgggttcgg gaatcgccgg
    12541 cattcaggcg tctctcgatt tggccaattc cggaatga
//
LOCUS       DAONQZ010000066        12277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5718_length_12277_cov_16.313697, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000066 DAONQZ010000000
VERSION     DAONQZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12277
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_12277_cov_16.313697"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLR13_09425"
     CDS             complement(<1..494)
                     /locus_tag="PLR13_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQC19600.1"
                     /translation="MKYEPLVIEEKWQKKWEDEKSFKVTEDPQKKKYYLLEMFPYPSG
                     KIHIGHVRNYTIGDVVARYKRMKGFNVLHPIGWDAFGMPAENAAIEHKIHPAKWTNEN
                     IEHMKKQLKRMGFSYDWDREVSTCEPKYYRWEQLFFIWMYEKGLAYKKRSTVNFCTKC
                     DTVLA"
     gene            684..836
                     /locus_tag="PLR13_09430"
     CDS             684..836
                     /locus_tag="PLR13_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19601.1"
                     /translation="MPLPVAAYQRRPQDSDYYHCVEDYFETFATETATPVIAGPKSLH
                     FISYHH"
     gene            complement(841..2202)
                     /locus_tag="PLR13_09435"
     CDS             complement(841..2202)
                     /locus_tag="PLR13_09435"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQC19602.1"
                     /translation="MKEGIVCKFGGTSVANAAQINKVKNIVTMNTKRQYVIVSAPGRD
                     SSDDQKVTDHLLNVATNGRHFRLQNKNITGQQSYEAVLGKFQRLIDELAIDGGDILKN
                     LEKDMKTPLPDVKRLDFMASRGERYNAEVVSRYFKKQGIPSELVLPEEMGLTVTDDFS
                     NARVIPVSYRNIKKKLSSIEGVSVIPGFYGVTEKGDIAVFSRGGSDLTGGEVAFSIEA
                     GLYENWTDTDGIYRADPRLIPEAKVIPRLTYKEIRLLSSKGFDVFHYDAMVNCKKRNI
                     PIQIRNTNNTNSEGTLIVSERVPEEVVVGIARKDNIAFIYIERDGAGETIGFVHDLLS
                     VIKDYGIETYHYPTDRDDMTILINQDDMVGYAEDIKERIEREFKPDTLEFNYNISILS
                     PVGIGMKNHPGVIAEAANALRDKNINIDIIIQGPAQISFHFGFQNFYADSALRAMYNC
                     LIE"
     gene            2587..4338
                     /locus_tag="PLR13_09440"
     CDS             2587..4338
                     /locus_tag="PLR13_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19603.1"
                     /translation="MAIDYSVIGNKIIMRTGGRLCETPDELLESDLFFRILTECIREL
                     ESRNSLLLNIFGKTTVDQQTIQKMALVLKNLAKIEAELIPKVVEGTEPYFSDINNLYK
                     FVEYLYNFWRSHERYIIIDSFDDSFEKRPYRVFNQRIQGLTELIRDTYRDIQENITGS
                     HPRIYRQLSAGAKISAICTREDIHLSGVYADKLSSVPMIRQVLFYPTLIFDPPMNKRT
                     GQFVRIDTNPMDVVDIRPDEWLCYPAMVGNLLMHVYFHQNFMDLGFSLCNLFELAGDK
                     HLAKKPDAIFLFGVQDGSLDGLAQYPTMFHEDRSNNLLIGACPGSSEFGYFGYLKKMM
                     LTLHNAIMIRQGRLPFHGALYQIHLKNGTEATILMMGDSGAGKSETLEAFRMLGDEHL
                     RGLQIIADDMGSINITEQGDIIGYGTETGAFIRLDDIKPGYAFRNIDRSIIMSPGKTN
                     ARIVLPVSTLEEVNMGVGIEYVLYANNYEEIDDDHPALERFSTPDQAFSVFREGTVMS
                     KGTTTSKGIVHSYFANIFGPPQYRKIYDQLARKSFEAFFRKGIFVGQIRTRLGIPGCE
                     MTGPKEAALKLLDMILA"
     gene            complement(4598..5044)
                     /locus_tag="PLR13_09445"
     CDS             complement(4598..5044)
                     /locus_tag="PLR13_09445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQC19604.1"
                     /translation="MGIHTYKAASVLKETGMMVESDARGFKFIADEPRGLGGTNRGMN
                     PMETILCALGACQCMTARFFAMLLKIDLTEYHVDVEGDIDARGFLNADSGIRPGFQEI
                     RLTVRMKANAPEEKIAELLAMVESRCPVGETVLHGAPVVVRHIELL"
     gene            complement(5312..6733)
                     /gene="purF"
                     /locus_tag="PLR13_09450"
     CDS             complement(5312..6733)
                     /gene="purF"
                     /locus_tag="PLR13_09450"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQC19605.1"
                     /translation="MLSEKEQEKRYYPREECGIFATYGHEEASRVAFYGLFALQHRGQ
                     ESAGIVSSDGCNVYEHKGMGLAAEVFKEETLQKLPGKLAIGHVRYSTTGSSNLCNAQP
                     FLVHYGNEYYALAHNGNILNAQKLRRELEDSGSIFQSTMDSEVIIHLMAHYLKQGLEK
                     ALAAALSRLEGAYCLIMLTRDKIIAARDPRGFRPLALGKVSGKWVIASETCAFDLVGA
                     DYVRDVEPGEILIIDATGVKSVNPFSAIRHCHCIFELIYFARPDSQVFRQNVYQYRKR
                     LGHELAREYKPDVDIVMPFPDSGVYAALGYAEESGRPFEMGMIRNHYIGRTFIQPSQA
                     MRDFGVRVKLNPVRALLENKKILIVEDSIIRGTTSRNRVKNLRNIGIKAIHMAVSCPP
                     TVYPCPYGIDFSSKGELVASKMESEKAIADFIGLDSIHYLSVEGMIRATGLKKDCFCL
                     ACFTGHYPIAPPEILDKFCLETK"
     gene            complement(6889..10095)
                     /gene="carB"
                     /locus_tag="PLR13_09455"
     CDS             complement(6889..10095)
                     /gene="carB"
                     /locus_tag="PLR13_09455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQC19606.1"
                     /translation="MPKRNDIKKILLIGSGPIIISQACEFDYSGTQACKALREEGYEV
                     VLINSNPATIMTDPEMADRTYVEPITPEAVEKIIARELPDALLPTLGGQTGLNTAVTL
                     AESGVLKKYNVEMIGASLDVIHKAEDREKFRHAIEKIGLRMPKSGFARSMQDVLKIAD
                     DIGFPIIVRASFTLGGTGSGVAYNREELMEIAKAGLDASMISEVMIEESVLGWKEYEL
                     EVMRDKADNVVIICSIENFDAMGVHTGESITVAPAQTLTDKEYQNMRDAAIAIMREIG
                     VETGGSNVQFAVHPQTGELIVVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMASFEPTIDYCVVKIPRWTFEKFPQAEDFLTTSMKSVGETMAIGRTFKE
                     SLQKAVRSLEIGRFSLEQALPDHIPDKKEFLKSKLIKPNSLRLFYIAAAMQNGLSIEE
                     LYRLTKIDPWFLHQIREIVDAENEIKESTPSVEVLREAKKMGFSDRALASFWKMTEPQ
                     IRQWREKGKIIPVYKLVDTCAAEFEAYTPYYYSTYETENETRLSDKRKVAIIGGGPNR
                     IGQGIEFDYCCVHASFALREEGVESIMINSNPETVSTDYDTSDKLFFEPVTLEDVLHI
                     LQTEKPEGVIVQFGGQTPLNLAKGLETAGINILGTSPDAIDRAENRDRFQEIVTKLKL
                     NQPDNDTARDTQKALQAAERIGYPVLVRPSYVLGGRSMEIVYDEETLKSFMEKALLVS
                     PEHPILIDKFLEDAVEIDVDAVSDGETTVVAGIMEHIEEAGIHSGDSACMLPPLTIPG
                     DVLDLIRKQTTMLAKELNVIGLMNIQYAVKDGKLYVLEVNPRASRTVPFVSKAIGVPL
                     AKLATKVMLGKSLKELGFTESPRPKHISVKEAVFPFNRFPDVDILLGPEMKSTGEVMG
                     IDQSFGLAFAKSQMATGFKIPQQGTVFISVNDQHKDRIVTVAKTFQKIGFKILATKGT
                     ALYLLEHGISAGVVLKLSEGRPNIVDFIKNGDMQIVINTSIGRKSSRDAYHIRHSALI
                     YNILYTTTIAGARALAEATVAIKEKAWQVCSLQEYQKA"
     gene            complement(10096..11226)
                     /gene="carA"
                     /locus_tag="PLR13_09460"
     CDS             complement(10096..11226)
                     /gene="carA"
                     /locus_tag="PLR13_09460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQC19607.1"
                     /translation="MFQLGKKRKALLVLEDGSIFAGNAFAGSGETMGEVVFNTGMTGY
                     QEVITDPSYKGQIVTMTYPLIGNYGINDEDRESAGIHLEGFIVKEYQAHPSNWRMTKS
                     LKSFLDDHGKIGIEGIDTRALTRRLRMAGAMKGIISTESANLESLLATLRDYPGLVGR
                     DLVREVTCSTPYQWERNAVRADGIAPDKPQHKLRVAVVDCGVKYNILRLLEKNGCEVV
                     VMPAFSTGDDIMSLKPDGVLFSNGPGDPAALSYIVDTARFIIGKVPVFGICLGHQIIG
                     QAIGGCTEKLKFGHHGINQPVKNAETGRVEITSQNHGFVVVLESMNDQAQKTYHNLND
                     NTSEGMSMPQAFSVQYHPEAAPGPHDTAYLFGRFVEMMKSVK"
     gene            complement(11298..>12277)
                     /locus_tag="PLR13_09465"
     CDS             complement(11298..>12277)
                     /locus_tag="PLR13_09465"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HQC19608.1"
                     /translation="RLGANEAPPAIISVFLGDQLIQILENIQRGTASKATDAQIIDLG
                     ISSLYQVSKDNTDRNRTSPFAFTGNKFEFRAVGSSQSISTQIIALNTIVAESIDDIAE
                     KIIARKDSKNIYQAVMDVLKDEITTIKPVLFNGDNYSKEWEAEAARRGLPNEKTTPTA
                     LKALITKKALNLFEKYEVLSNIELKSRQLIHIERYIKDLEIEVKCLNSMCMSYILPAA
                     VIYQKRLAGAINATREALGTEAVVSAEIELLKKISDLINNIFATNRDIQQKVESANAL
                     HDEQKKAEMLSAKVKPMMEELREYADALEILVDDDIWPVPKFWEMLFIC"
BASE COUNT         2989 a         3129 c         2888 g         3271 t
ORIGIN      
        1 gccagcaccg tatcgcactt ggtgcagaaa ttaacggtgg aacgtttttt ataagccagg
       61 cctttctcat acatccagat aaagaaaagc tgttcccacc ggtaatattt cggctcgcaa
      121 gtggaaactt ccctgtccca gtcgtagctg aagcccatgc gtttgagctg ttttttcatg
      181 tgctcgatat tttcgttggt ccatttcgcg ggatgaatct tatgctcgat ggcggcgttt
      241 tccgcaggca tgccgaaggc atcccagccg atcggatgca gaacattgaa acctttcatg
      301 cgtttgtagc gggcaacaac atcgccgatg gtgtaattgc ggacatgacc gatatgaatt
      361 ttgccggagg gataagggaa catctccagc agataatatt ttttcttttg cggatcttcg
      421 gtaaccttga aggatttttc atcctcccat ttcttttgcc atttctcttc gataactaac
      481 ggttcgtact tcatttttca aaatctctcc ggatggtgaa cgttgctgat ttgcttaaca
      541 tatagacacg atgagcgcaa tggctatatc gaaaacactg tacgatttga cacatcatta
      601 ttttcttctt aacaatagga cttttgtcct attaagtctt gtggcttcgg aatgcattta
      661 tccgttttat ggaagaggat tatatgccat tgcccgtagc cgcataccag cgccgaccgc
      721 aggattcgga ttattatcat tgcgtggagg attactttga aacttttgcg actgaaacgg
      781 caacgccggt catcgccggc cccaaaagct tgcacttcat cagctatcat cactgacgct
      841 ttattcgatc agacaattat acatggcgcg cagggcggaa tcagcataga agttctgaaa
      901 tccgaagtga aagcttatct gcgcaggtcc ctggatgatg atatcgatgt tgatgttctt
      961 atcacgcaat gcattcgccg cttccgcgat aacgccggga tgatttttca tgcctatgcc
     1021 tacgggagaa agaatagaga tgttataatt gaattcaagg gtatcgggtt tgaactcccg
     1081 ttctatccgt tccttgatgt cctctgcgta accgaccatg tcatcctgat tgatcaagat
     1141 ggtcatatcg tcccggtctg tcgggtaatg atatgtctca atcccataat ccttgataac
     1201 actcagaaga tcgtgcacaa aaccaatcgt ttctcctgct ccgtcacgtt ctatataaat
     1261 aaaagcaata ttatctttac gggctatacc gacgacaact tcttcaggaa cccgttcgga
     1321 tacaataagc gtgccttcag aattcgtgtt attggtattt cggatctgaa taggaatatt
     1381 ccttttcttg caattcacca tggcatcata atgaaacaca tcgaagccct ttgatgaaag
     1441 cagacggatt tctttatagg taaggcgggg gatcacctta gcttccggaa tcaggcgcgg
     1501 atccgcccga tagatgccgt ccgtatcggt ccagttttcg tacagacccg cctcgatgga
     1561 gaaggcgacc tcgccgcctg tcagatcgga accgccgcgt gaaaatacag caatgtcgcc
     1621 tttttcggtc accccgtaaa aaccgggaat gacggatacc ccttcaatgc tgctcaattt
     1681 tttcttgata ttccgataac tgactggaat cacccgggcg tttgaaaaat cgtccgtgac
     1741 cgtcaacccc atttcctcag gaagaacaag ttctgaaggt atgccttgtt tcttgaagta
     1801 acggctcaca acctcggcgt tatagcgttc acctctggat gccatgaagt caagccgctt
     1861 aacatccggg aggggtgttt tcatatcttt ttcgaggttt tttaatatat cgccgccatc
     1921 aatggccaac tcgtcaatca gccgctgaaa tttccccaga acagcttcgt aactttgctg
     1981 acccgtgatg tttttgttct gcaggcgaaa atgtctgcca ttcgttgcaa cattcaacag
     2041 gtggtcagtt actttctgat catccgaaga atccctaccc ggtgcggaaa caatcacata
     2101 ttgccgtttc gtgttcattg tgacaatatt tttaaccttg ttgatctgtg cggcattggc
     2161 aacagatgtc ccgccaaatt tgcatacgat cccttctttc atacgcttct ccttgaagtt
     2221 ttatttatca gcgacaccgg ttcttgggtt tttttgacat gcacaaacgc cctcaaaatg
     2281 tgttttatta tacacaaatt tgacgtcgat gtctatcagc cgaacaccac tgggaaggca
     2341 ttctctttgt catatccttt atttaaagga aattttagca ataaaaacgc aaggaacaac
     2401 agatggacga ctatgataat ttcgatagca atatcgactg aaaaaataac gtccactccg
     2461 gtatacgcat cgttcaatct gttatcaaga tatcacttgc ggcatatgcc ccttgagaaa
     2521 aggattgatt tcatcggggg aacagtgata tttttcaacg aaacgacgaa cgaaaggagg
     2581 ggaaccatgg ctatagatta ttcagtcatc ggcaataaaa taatcatgcg aacagggggc
     2641 cggctttgcg aaacccccga cgaactcctg gaaagcgatc tgttcttcag aatcctaaca
     2701 gaatgtatca gggaactcga atcgcgaaat tcgctgcttc tgaacatatt cggaaaaacg
     2761 accgttgatc agcagactat acaaaaaatg gcactggtgc ttaaaaatct tgccaagata
     2821 gaggctgaac ttattccaaa agtagttgag ggaaccgaac catatttttc cgacatcaac
     2881 aatctataca aattcgtgga atatctatac aatttctggc gctctcatga acgctacatt
     2941 ataatcgatt catttgacga tagtttcgag aaacgaccct accgggtgtt caaccagagg
     3001 attcagggcc tcactgaatt aatccgagac acatatcggg atatccagga aaatattacc
     3061 ggatcacatc cccgcatata ccggcagctg tctgccgggg ccaagatttc cgccatctgc
     3121 accagagagg atattcatct ctccggcgtt tacgcggaca agctctcatc ggtaccgatg
     3181 attcgacagg tattattcta tccgactttg atcttcgatc cacccatgaa caagcggacc
     3241 ggccagttcg tccgcatcga cacgaaccct atggatgtag ttgatatccg gccggatgag
     3301 tggctctgct accccgccat ggtcggaaat cttctcatgc atgtctattt ccaccaaaat
     3361 tttatggatc tggggttttc gctctgcaac ctgttcgaac tcgccgggga caagcatctc
     3421 gcgaagaaac cggatgcaat tttcctgttc ggcgttcagg atgggtccct tgacggactg
     3481 gcacaatatc cgaccatgtt tcacgaggac cggtccaaca acctgttgat cggagcttgt
     3541 ccgggaagca gcgagttcgg ttatttcggc tatctcaaga agatgatgct tacattgcat
     3601 aacgcgatca tgatcaggca ggggagactc cctttccacg gcgcgctcta tcagattcat
     3661 ttgaagaacg ggaccgaagc gaccattctc atgatgggcg attcgggcgc gggcaaatcc
     3721 gaaacactgg aggcgttcag gatgctcggg gacgagcacc tgcgtggact gcagattatc
     3781 gcagacgata tgggttccat caacattacg gagcaaggcg atataatcgg atatgggacc
     3841 gaaacaggcg cgtttataag gcttgacgac atcaagcccg gatacgcttt caggaacatc
     3901 gaccggtcga tcatcatgag ccccgggaaa acaaacgcac gtatcgtatt gccggtttca
     3961 accctcgaag aggtcaacat gggggtcggg atcgaatacg ttctctacgc caataactat
     4021 gaggaaattg acgacgacca tccggcactc gaacgcttct caacacccga tcaggcgttt
     4081 tcagtgttca gggagggcac ggtcatgagc aaggggacca ccacatcgaa agggatcgtc
     4141 cactcgtatt tcgccaacat tttcggcccg cctcagtatc ggaaaatata tgatcagctc
     4201 gcgcgtaaat ctttcgaagc attcttcagg aagggcatct tcgtcgggca aatccgtaca
     4261 cgcctgggta tacccggatg tgaaatgacc ggcccgaaag aagcagctct gaaacttctt
     4321 gacatgattc tggcctagta tgcagaacgc ctgaagaaca agaaatttga accaatcaat
     4381 accttgtatc catcaatatc aattcaatcc attattttgt ctaatccgtg ggggacagtc
     4441 caaaatatct ttatatgcga ggaaatctca accaccgaat tatctcaaat aaaatgttac
     4501 taaagggtgt cgtccacaag gatacgttct ttgaggaaaa tgctaaagac aagattcttc
     4561 atttataagt tcccgaggtc agttcgttga tcgtcaacta taataattcg atatgccgaa
     4621 cgacaacagg cgcaccatgg agaacagttt caccgacagg gcagcggctt tccaccatag
     4681 ccagcagttc agcgatcttt tcctcagggg cattggcctt catacggacg gtcaagcgga
     4741 tttcctgaaa gccgggacgg atgcccgaat cggcattgag aaagcccctg gcatcgatat
     4801 ctccctcaac atccacgtgg tattcggtaa gatcgatttt aaggagcatg gcaaaaaaac
     4861 gggccgtcat gcactggcag gcgcccaggg cgcacaagat ggtttccatg gggttcattc
     4921 ccctattggt tccgccgagc cccctgggtt catcggcgat aaatttgaat cccctcgcgt
     4981 cgctttccac catcattccc gtttctttta aaactgaagc cgctttatat gtatgtattc
     5041 ccatcatttc tcctgtttac gaactatcca ttcagacaga ttactgcaaa acggacgtat
     5101 gaataaatcg ttgattgccc ggttaacttt tccggatctt tcaacgggtg atatgctccg
     5161 tcaccgttat ataatctaat gtgctccgga aattttttca atcagtaatt atccttattg
     5221 gaaggaattg gggtcaaatt caattcaacc aattatcaat caataatttc aattgctcac
     5281 gacaatcgag gcaggaattt atccggaaaa actattttgt ttccagacaa aatttatcaa
     5341 ggatttcggg cggagcaatg ggatagtgac cggtaaagca ggccaggcaa aagcagtctt
     5401 ttttcaggcc ggtcgccctg atcatccctt cgacggacag ataatgaatg gagtccagac
     5461 cgataaaatc agcaatggct ttttcacttt ccatcttgga ggcgacaagt tctcctttgg
     5521 atgaaaaatc aatgccgtac ggacagggat agaccgtcgg cggacagctc accgccatgt
     5581 gaattgcttt gatgccgata ttgcgcagat tcttgacccg gttgcggctg gtcgtgccgc
     5641 ggatgatgga gtcctctacg atcaggattt ttttattttc cagcaaggct ctgacgggat
     5701 tgagtttcac gcggacgccg aaatcgcgca tggcctgtga aggctgaata aaggtgcggc
     5761 caatatagtg gttgcgaatc atacccattt caaaagggcg gccgctttct tcggcataac
     5821 ctaaagccgc gtaaacgccg gaatcgggaa acggcatgac gatatcaaca tccggtttgt
     5881 attcacgcgc cagttcgtgt ccaagccgct tgcgatactg ataaacattc tgccgaaaaa
     5941 cctgactgtc gggacgggcg aaataaatca gttcaaaaat gcaatggcag tgcctgatcg
     6001 cggaaaaagg gttgacgctt ttgacgcccg tggcatcaat gattaaaatt tctccgggct
     6061 ctacatcgcg aacatagtct gcgccgacca ggtcgaacgc acaggtttcc gaggcgatca
     6121 cccatttgcc gctcactttg cccagcgcca gcggcctaaa tccgcgcgga tcacgggcgg
     6181 ctatgatttt gtcgcgggtc agcataatca ggcaataggc gccttccagt cgcgatagtg
     6241 cagccgccag cgctttctcg aggccctgtt tcaggtaatg tgccatcaga tggataatca
     6301 cttcgctgtc catggtggat tgaaagatgg aaccgctgtc ttcaagctcc ctgcgtaatt
     6361 tttgtgcgtt gagaatattg ccgttatggg ccagagcgta atattcgttt ccgtaatgca
     6421 ccaaaaacgg ctgggcattg caaagattcg atgaaccggt cgtggagtaa cggacatgtc
     6481 cgatggccag cttgccgggc agcttttgga gcgtttcttc cttgaaaacc tcggccgcca
     6541 gccccatgcc tttgtgctcg tatacattgc agccgtcaga gctgacgatg ccggcggatt
     6601 cttggccgcg atgctgaagg gcaaaaagac cgtaaaacgc tacgcgggat gcttcttcat
     6661 gaccgtaagt ggcaaaaata ccgcattctt cgcggggata atatcgcttt tcctgttctt
     6721 tttccgataa cattttatca aaaaaccttt tggattttca ttactctcgc attctgattt
     6781 tttcctctcc ccgcggtaac aaatcgaggg gagaggctat tttttaattg tcactctttt
     6841 ctaccctata ggacaccata accttcaaag gtgagaaaat aagctgtttt atgctttttg
     6901 atattcctgc agggagcaaa cctgccaggc cttttctttt attgcgactg tcgcttcagc
     6961 cagggcgcgc gctccggcaa tggtcgttgt ataaaggata ttatatatca gcgcgctgtg
     7021 cctgatatga taagcatccc tcgaagattt tcgtcctata ctggtgttaa taacaatctg
     7081 catgtccccg tttttgatga agtccacgat attcggtcgg ccctcgctta actttaaaac
     7141 aacaccggcg ctgataccgt gttccaaaag gtacagggca gtgcctttcg ttgccagtat
     7201 tttaaaaccg atcttctgaa acgttttggc gaccgttaca atcctatcct tgtgttgatc
     7261 attgacgctg atgaagacag tgccttgctg cggtattttg aatccggtcg ccatttgcga
     7321 tttggcaaag gcaagtccga aagattgatc gatgcccatg acttcgccgg tggatttcat
     7381 ttccggcccg agcaaaatat ccacatccgg gaaccggttg aacggaaaga cagcttcttt
     7441 aacggaaata tgttttggcc ggggggactc ggtaaatccc agctctttta aagacttgcc
     7501 cagcatgact tttgttgcca gtttggccag cggcacaccg atggccttgc tgacaaacgg
     7561 caccgttcgc gaagcgcggg gattaacttc caacacataa agtttcccgt ctttgacggc
     7621 atattgaata ttcatcagac cgatcacatt caattccttc gccagcatgg tggtttgttt
     7681 ccggatcaga tccaaaacat caccgggaat ggtcaaggga ggcaacatgc aggcgctgtc
     7741 accggaatga atgcccgcct cctcgatatg ctccatgatc ccggccacca ccgtggtctc
     7801 accgtcgctg acggcatcca cgtcaatttc cacagcgtcc tccagaaact tgtcgatcag
     7861 gatggggtgt tccggcgaga ccagcagcgc tttttccatg aaggacttca gcgtttcttc
     7921 atcgtacacg atttccatgg aacgcccgcc cagcacgtag gaaggccgca ccagcaccgg
     7981 atagccaata cgctcggcgg cctgcaatgc tttttgtgtg tctcgtgccg tgtcgttatc
     8041 cggctgattc agtttgagtt tggtcacaat ttcctggaag cggtcgcggt tttcggcccg
     8101 gtcaatggca tcgggcgatg ttcccaggat atttatgccg gcagtttcca agcctttggc
     8161 cagattaagc ggcgtctggc cgccgaactg cacgatcaca ccttccggtt tttccgtctg
     8221 taaaatgtgc aaaacgtctt ccagcgtaac cggttcgaaa aacaatttat ccgacgtatc
     8281 gtaatcggtg ctgaccgttt ccggattgga attaatcata atgctctcaa cgccttcctc
     8341 gcgcaaggca aacgaggcgt gaacgcaaca ataatcaaat tcaatgccct gaccgattcg
     8401 attcggtccg ccgccgataa tggctacttt gcgtttatcc gacagccggg tttcgttctc
     8461 cgtctcgtac gtggaataat aataaggcgt gtaggcttca aattccgctg cgcacgtatc
     8521 gaccagttta tacactggaa tgatttttcc cttttcgcgc cattgtctga tttgaggctc
     8581 cgtcattttc caaaaagacg ccaacgccct gtcggaaaag cccatttttt tagcctcgcg
     8641 cagcacctcc accgatgggg ttgattcttt gatttcattt tcggcatcaa cgatttccct
     8701 gatctgatgc aaaaaccagg gatcgatttt ggttaatcga taaagttctt cgatcgacag
     8761 gccgttttgc atagccgcgg caatgtagaa aagccgcagc gaattgggct tgatcagttt
     8821 gcttttcaga aattcttttt tatccggtat atggtcaggc agagcctgtt cgagagaaaa
     8881 acgcccgatt tccagcgacc gaacggcctt ttgcagtgat tccttgaatg tgcggccaat
     8941 ggccatggtc tcacccacgg atttcatgga tgtagtcaga aaatcctcgg cctgcgggaa
     9001 tttttcaaat gtccagcggg gaatttttac gacacagtaa tcaatcgtcg gttcgaaaga
     9061 cgccatcgtc tcgcgggtaa tatcgttggg aatttcatcc agcgtgtaac cgacagccag
     9121 tttcgccgca atcttggcaa tgggaaatcc tgtcgctttg gaagccagcg ccgaggaacg
     9181 tgaaacgcgg ggattcattt ccacgacaat cagttctccg gtttgcggat gcacggcaaa
     9241 ctgaacattg gacccgcccg tctctacgcc gatttcacgc atgatggcaa tggcggcgtc
     9301 ccgcatgttc tgatattcct tgtccgtcaa cgtctgcgcc ggtgcgaccg tgatagattc
     9361 accggtatgc acgcccatcg cgtcgaaatt ttcaatggag cagataatca cgacgttgtc
     9421 agccttgtcg cgcatcactt ccagttcata ttccttccag cccagcaccg actcttcgat
     9481 catgacctcg gagatcatgc tggcatcaag tcccgccttg gcaatttcca tcagttcttc
     9541 ccggttatag gccacaccgc tgccggtgcc gcccaaggta aacgacgccc ggacgataat
     9601 cggaaacccg atgtcgtcag cgattttcag aacatcttgc attgaacggg caaaaccgct
     9661 tttcggcatg cgcaaaccga ttttttcaat ggcgtgccgg aatttctccc ggtcttccgc
     9721 cttgtgaatg acgtccagcg aggcgccgat catctcgaca ttgtatttct tgagcacacc
     9781 gctttcagcc agcgtcacag ctgtgttgag tcctgtctgt ccgcccagcg tcggcagcag
     9841 cgcatcaggc agttcgcggg caatgatttt ttccacggct tccggtgtga tgggttcgac
     9901 gtaagtgcga tcggccattt ccggatcggt catgatggtg gcgggattgc tgttgattaa
     9961 gaccacttcg taaccctctt cccgcagcgc cttgcaggcc tgtgttccgg agtaatcgaa
    10021 ttcgcaggcc tgactgatga tgatgggacc ggaaccgatc agcaggattt tctttatgtc
    10081 gtttcgtttc ggcacttatt tcacactttt catcatttca acaaaccgcc cgaacagata
    10141 tgccgtgtcg tgcggtcccg gagccgcctc cggatgatac tggacgctga aggcctgcgg
    10201 catgctcatc ccttcagagg tgttgtcatt caaattgtga tacgtttttt gagcctgatc
    10261 gttcatcgat tccaaaacca ccacaaaacc gtgattctgc gacgtgattt cgacccgtcc
    10321 cgtttcggca tttttcaccg gctggttaat gccgtgatga ccgaacttta atttttccgt
    10381 acagccgccg atcgcctgac cgataatctg atgccccaga caaatgccga aaaccggcac
    10441 cttgccgatg atgaaacggg cggtatccac aatataagaa agagccgccg gatcgccggg
    10501 gccgttggaa aacagaaccc cgtccggctt gagcgacatg atgtcgtcgc cggtggaaaa
    10561 agcgggcatc acaacgacct cacagccgtt tttctccaga agtctcagga tattatactt
    10621 cacgccgcaa tccacaacgg ccacgcgcag tttatgttgc ggtttgtcgg gcgcaatgcc
    10681 gtctgcccgg acggcattgc gctcccactg ataaggggtt gaacaggtca cttcgcggac
    10741 cagatcgcgg ccaaccaaac cgggataatc ccttaacgtc gccagcagcg attccagatt
    10801 cgcgctttcc gtggaaatga ttcccttcat ggcacccgcc atgcgcagac gtctggtcaa
    10861 agcacgggta tcaatacctt caataccgat tttgccgtga tcgtccagaa acgatttcag
    10921 gcttttggtc attcgccagt tgctgggatg agcctgatat tccttgacga taaacccttc
    10981 cagatgaatg ccggcggatt ccctatcttc atcattgatg ccatagttcc cgatcagggg
    11041 atacgtcatg gtaacaatct ggcctttata ggaaggatcg gtaatcactt cctgataacc
    11101 ggtcatgccc gtgttgaaga cgacttcccc catggtttcg ccagaccctg caaaagcatt
    11161 tcccgcaaag atactgccgt cttccagaac caataacgct tttctttttt tacccagttg
    11221 aaacatcgtt aacttccctg ttgcataaaa tcggttgcgg caaaacgtga catggcgaaa
    11281 aaatgatggc ctccatatca acagataaac aacatttccc agaatttcgg aacaggccag
    11341 atatcgtcat caaccaggat ctccagggca tccgcatact cgcgcagttc ctccatcatt
    11401 ggcttgacct tggcactcaa catttctgct ttcttctgct catcatgcag agcgttggcg
    11461 gactccactt tctgctgaat gtccctgttt gtagcgaaga tattgttgat cagatcgctg
    11521 attttcttga gcagttcgat ttccgccgag acaacggcct cggtgcccag agcttctctg
    11581 gtggcattga tggctccggc cagtcgtttc tgatagatta cggctgccgg caggatatag
    11641 ctcatacaca tggaattgag acatttgact tctatctcca gatccttgat gtagcgctcg
    11701 atgtggatca actgccgtga tttgagctca atgttggaaa gcacctcgta tttttcaaaa
    11761 agattcagtg ctttcttcgt aatcagggcc ttcagggcgg tgggggttgt cttttcgttc
    11821 ggcagacccc tccgggccgc ttcggcttcc cactccttgc tgtagttgtc accgttaaac
    11881 aggaccggct tgatcgtggt gatttcgtcc ttcaaaacat ccatgacggc ctgatagatg
    11941 tttttggaat cttttctcgc tatgattttt tccgcaatat cgtcaatgct ttcagcaacg
    12001 atcgtgttca gagcaatgat ctgggtggaa atagattgcg acgagcccac agccctgaat
    12061 tcaaatttgt tgccggtaaa ggcaaaaggc gatgtcctgt tgcggtccgt attgtctttg
    12121 ctgacctgat acagggaaga aatgcccaga tcgatgattt gcgcgtcggt cgctttcgaa
    12181 gcggttcctc gttgtatgtt ctccagaatt tggatcagtt ggtcccccag aaacacggag
    12241 atgatggccg gcggcgcctc gttggcaccc aggcggt
//
LOCUS       DAONQZ010000067        12165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5787_length_12165_cov_14.209166, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000067 DAONQZ010000000
VERSION     DAONQZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_12165_cov_14.209166"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..647
                     /locus_tag="PLR13_09470"
     CDS             372..647
                     /locus_tag="PLR13_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19609.1"
                     /translation="MDDEILLNQLEELAERLGILVRDENINIDDVSTTGGLCIVEGQH
                     ILILNSKTSLQEKIHVAIRALRQFDLSEIYIKPGIRELLEEDKQYEH"
     gene            complement(1220..2149)
                     /locus_tag="PLR13_09475"
     CDS             complement(1220..2149)
                     /locus_tag="PLR13_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19610.1"
                     /translation="MSATVKTELTLMQLYPHAKKPRHFMMGLLTLIFIIPAGYIAFIL
                     LLGLKIAFYAPLYLGLMLPMNRASAGRIWELKNRALLPVHHIIYKLYYIHIKLWLSCF
                     NDYDAFWYGLDVTRYGSHADFLSSYRDSRVRWRFKKKLKTYRSHGITEDVLPDHTVFF
                     RTLFSFRYFILICRSNFRKNRGHAFLFGHYLVLRDYFILLFLPVRVHVYSKEGRAVGI
                     ATYLKRNNTLVMCQHIIADEYIRSGIFYSQMNTCMAYAFRDPAVRYVSCSITTPQAKQ
                     TCGCYPINYLLTDEFKYLPFTQLDTLGADAIEK"
     gene            complement(2410..2961)
                     /locus_tag="PLR13_09480"
     CDS             complement(2410..2961)
                     /locus_tag="PLR13_09480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQC19611.1"
                     /translation="MSHYQIAVVVGSLRQDSFNRRLADAIVKLAPPDFSFRQARISDL
                     PLYNQDDDAHQAEAVKRLKNEIKNAHGVLFVTAEYNRSIPGVLKNAIDHASRPYGQNV
                     WAGKPAGVIGISIGVIGTAMSQQHLRNVLAFLDMPTMGQPEVFLQAKDGLFDETGNIG
                     AGSKQFLQDWMDKYVAWVKKHAA"
     gene            3327..3602
                     /locus_tag="PLR13_09485"
     CDS             3327..3602
                     /locus_tag="PLR13_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19612.1"
                     /translation="MKKTLGTIIVAAAVVLLTATFSFAEYAAAGAANFPFFQLGCLVV
                     GGLILVSLKRKYEKMYIGEVVGVFALYTILMALFTNPVIEVVKTIVT"
     gene            4170..4571
                     /locus_tag="PLR13_09490"
     CDS             4170..4571
                     /locus_tag="PLR13_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19613.1"
                     /translation="MKKAFLQSNRVVWRVILPGQCRSLLLVVLIVQSFLLCSCASTPS
                     ESFLKEAAIYSCYGGGSYADTVLNEYDKNTVRLEKFKVTEKYDKKIDDENYTIYKYLI
                     SVHDTKTQLTDNLSCSAAVIKRGPKWYYRLP"
     gene            4919..6379
                     /locus_tag="PLR13_09495"
     CDS             4919..6379
                     /locus_tag="PLR13_09495"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQC19614.1"
                     /translation="MNSNLTTMWNAMQSGMGNHIPALLGALAVLVLGWLLAVLARAGV
                     RRGLGKLNLNTRFAQLTGQSINLENGLAIAIFWIVMLFIITAVLNSLDLAMVSAPFAA
                     LLTQFLAYIPHLLAGLMLALIAWLLASIVRRFVTKALSKTTLDEKLSEHAGMSPISDG
                     LSQALFWLIILLFVPAIMGALQMDGLLDPFRDMITKVLDFLPNAAAAVAIGFVGWILA
                     TVLRNLTTNLLRTAGSDKLSQKVGLAESVKLSNAIGLLVFVAVFLPALIAALDALKIE
                     AISKPAMDMLDMILMAVPHIVAAGLILVITWMVASFASRLLASLLNAMGFDNIPKHIA
                     LEHAFQKTPASVFVGYVVLFFAMLFATVEAANQLGFTHVSDTVDTFIRFGGDVLLGSL
                     ILVIGFWLANLAYEAMDKASGPNTKGLARIGRFAILGLVLALGLRAMGIADDIVNLAF
                     GLTLGAIAVTVALSFGLGGREPAGKLMEHWLSRFRQ"
     gene            6756..7364
                     /locus_tag="PLR13_09500"
     CDS             6756..7364
                     /locus_tag="PLR13_09500"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HQC19615.1"
                     /translation="MRSNRLPNHNTHCSRCYQRREICLCPIIPAVETSVEFLILRHIY
                     EAEKPSNTGRLAALALTNSKLISCGGGQRIGLACVDDDLLNAPGTWLLWPDGAGTLPR
                     TSDRQSPARVVVIDATWQQARRLYSSLPALWRMPRLALEAPVAQRKRLRDQHRPNYMS
                     TLEAVAAAVAMLEGGGKNVPLDNLYDELVRRRRSLRWGSKES"
     gene            7910..8548
                     /locus_tag="PLR13_09505"
     CDS             7910..8548
                     /locus_tag="PLR13_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQC19616.1"
                     /translation="MENKNCKNKRVHVVLFDFGGVLSEEGWKKGLRIIAKANGLHPDS
                     FLQTAADTIYETGYILGKGSQSDFWKSLKTKTGIRGDVASLSGELISRFALNDAMMET
                     VRNLKSNNFIVGILSDQTDWLEGLNARFDFFRHFDYVFNSYHLGKGKRDPSLFDDIAG
                     LLRETPENILFIDDDPGNIQRARQKGWKAILYTDFKSFREEINEIIQERTLV"
     gene            8517..8663
                     /locus_tag="PLR13_09510"
     CDS             8517..8663
                     /locus_tag="PLR13_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19617.1"
                     /translation="MKSYRKELWFNTAHRREFINITGQVEDCLRESGIREGLALINTT
                     KEYF"
     gene            9150..9407
                     /locus_tag="PLR13_09515"
     CDS             9150..9407
                     /locus_tag="PLR13_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19618.1"
                     /translation="MSEAFTRESIREHHRVKIKKIINQGGGVEEIDNYLMNRISTVIS
                     YERRKNERRQLRKVTKNRSVFSNDEALTEILYLAIRDVMKK"
     gene            9662..11452
                     /locus_tag="PLR13_09520"
     CDS             9662..11452
                     /locus_tag="PLR13_09520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQC19619.1"
                     /translation="MTVYKRRLWLLCLVTGIVFHCVHAHALEPTFLKDSPKVNICRAL
                     EFLEDPAGELSLEQLLHPGTQDRFVPLNDPQLNFGITRSTYWFRFLLKFDQSENSCEY
                     ECLDNNSWLLEIDRAWMPTIELWVPVIRDSHDIRKTAQIMGESWRKLSAVTSDQSNRI
                     NILNRSSIFFIPKDYDESRPMYLKMASWGPPLNFSLHVRRLNEFRNWLTWDFTVFGII
                     YGLIIAMTLYNACMYLSLRDKTYLVYVLYIFSNFLYLSIGLGHFSALTGILSGSHFYL
                     IYVFTGSAGFFAAWFCRTFLLTSKNAPLIDKILMAYMFFAILLILMAVTGFYYQVQIL
                     GAAIGLTFPIMAITAGLVCFIKGMKPAGYFLTAWSVLSVGMLTWAFRSLGILPQTQLI
                     TYSYPIAVAAESILLSLALADRIRTLQQENRELENREHRLLILSNTDGLTKLYNKRYF
                     NEKLKSEISLSGNLGDPLSLLILDLDDFKKINDAYGHPAGDQVLKRLGEILLMSIRDK
                     DYGCRYGGEEFTVILPGAEKEKALDVAERIRRLISDDTFETSDGQKFSVTVSIGLAQL
                     RPGENSADLINRADQSLYSAKRQGKNQTVA"
     gene            11501..11923
                     /locus_tag="PLR13_09525"
     CDS             11501..11923
                     /locus_tag="PLR13_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HQC19620.1"
                     /translation="MKFLRYIWALPNTTLGLLFVPLALFTRGGMHVVNGVLELHSRLI
                     SLILKKCMPVRGGVSAITLGQNRESLSINRRHEHAHVHVRQYEMLGPIFIPVSEKLIS
                     WLTENQIKVLNVAGPRLRKDPTIYDAVLRLLKVTLSAA"
     gene            11936..>12165
                     /locus_tag="PLR13_09530"
     CDS             11936..>12165
                     /locus_tag="PLR13_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HQC19621.1"
                     /translation="MKSFRKELWFNTKKRREFINITSQVEDCLEESGIHEGIVLVNAM
                     HITASVFINDDESGLHQDYENWLENLAPHEPVA"
BASE COUNT         3384 a         2581 c         2907 g         3293 t
ORIGIN      
        1 tgcgctgcgc ttgtcatatt ggagtctcat tataatgcga cgcacgtttg tcgtccgggg
       61 atggtgttat cgagcaaggc ttcgtatagt gaatgcaaat ctcaggcagc tttaatacga
      121 caatgatgac acccatagaa tcaattttta aaatacagtc ctctgtaatg gatcttcaga
      181 gatacctgaa tgccagcgac cctatggttc ccagggtggc acggacaaaa tatataaaat
      241 atgtgaacag atttttcagg gaacatgtta agtgggtgga atatgaaaag cgtgatcttt
      301 gcctggatga tgtcaattat tttttaaacc tgctggaatc aacggtggaa cattattatc
      361 agagttaaaa catggatgac gaaatccttt taaatcagct tgaagaactg gctgaacgac
      421 tcggaatact ggtccgtgat gaaaatatca atattgacga tgtatccacc accggaggac
      481 tatgcattgt cgaaggtcaa catatcctga ttctgaattc caaaacctca ttacaagaaa
      541 agattcatgt tgcgatccgg gcattaaggc agtttgacct cagtgagatt tacataaagc
      601 cgggtatccg ggaattgttg gaagaagata agcaatatga acattgaaaa tcagcttgcc
      661 catgcgttga aaactacgcc atcgtctcta atccgattag tttaaaaaag catttagaat
      721 aacgctactg ttgtatagat gtattaatta tttgcataaa gtgacgaata gtatcagttt
      781 atgcaaacga tagatacaaa tggaggcttt gtgctagttg attttataaa tgcaatggct
      841 gacatggcca caaaactggc agaccgcctc gacccattgg tcaagtgatc tgtctatgag
      901 agggattcat ttgaatagtc atccctttca ttgtctttgt cagattgata tttttgaaca
      961 ataaatgtca tagcaaaagc aggagaggtc aaaatatggg ggcatatttg ggggtatgag
     1021 gaaaaagatg aattgagata actcaataat tcaaaacagt tatagaaaat gttcgactcc
     1081 cggcgcttcg ttttgctctt acaaatccta tatcaatgaa gtatcaattc gattgaactg
     1141 attaaaattt ttccctttgg atgtagaaaa aataacttgt atttaattct gcaagccgat
     1201 cagagggtac tgtaatttcc tacttctcta ttgcgtctgc cccgagtgtg tcaagttgtg
     1261 taaatggcaa atatttaaat tcatctgtca ataaataatt gatgggatag cagccgcagg
     1321 tttgttttgc ctgcggcgtg gttatcgagc atgaaacgta gcgtacagcg ggatccctga
     1381 aagcatacgc catgcaggtg ttcatctgac tgtagaaaat acctgaacgg atgtactcat
     1441 cggcgatgat atgctggcac atcaccagcg tattgttccg cttcaggtac gtggcaattc
     1501 cgactgctct cccttccttg gaatacacat gcacccgcac gggaaggaaa agaaggatga
     1561 aatagtccct caagaccaga taatgaccaa aaaggaaagc atggcctcta ttctttctaa
     1621 aattggacct gcagatcaat ataaaatatc tgaaagaaaa aagcgtcctg aagaagaccg
     1681 tgtgatccgg aagcacgtcc tcggtaatgc cgtgtgaccg gtacgttttc agtttttttt
     1741 tgaatctcca ccttaccctg gaatcgcggt aggatgacag aaaatcggca tggctcccgt
     1801 atcgggttac atcgaggcca taccagaagg catcataatc attgaagcag gaaagccaca
     1861 gcttgatgtg gatgtaatac agcttgtaaa taatgtgatg aacaggcagc aatgccctgt
     1921 ttttcaattc ccatattcgc ccggcagacg cccgattcat gggtaacatc aaccccagat
     1981 ataaaggggc atagaatgca atttttagcc cgaggagcag aataaaggcg atatatccgg
     2041 ccggtatgat aaaaatcaac gtcaacaatc ccatcatgaa gtgccgcggt ttttttgcat
     2101 ggggatataa ctgcatcagg gtcagttcgg ttttgacagt ggcactcata atgttttgtc
     2161 catggatcaa tttgaatgac ctgcgtttat cttaaataat aaaattgaat taccaaaggt
     2221 gcgctgaaac ttgcaaagcg caatgggact caaaattcag aaatcaaatg aactgaattt
     2281 tataagtcga attatccgat ccgacaggcc gggaagggaa attatcctgc aacagcacgg
     2341 atataatatc ccgcggcttg ctgcgaaatc cgggaccagg gtttgcctgg cggttcatac
     2401 ccgcgatcat caggccgcgt gctttttcac ccaggcaaca tatttatcca tccagtcctg
     2461 caggaattgt ttgctgccgg cgccgatgtt acccgtctca tcaaacaatc catcctttgc
     2521 ctgaagaaat acttcaggct gacccatcgt cggcatatcc agaaaagcaa gaacgtttcg
     2581 caaatgctgc tgggacatgg ccgtaccgat aacgccgatg gaaatcccga tcaccccggc
     2641 cggtttgcct gcccagacgt tctgcccgta aggacgggag gcgtgatcga tggcgttttt
     2701 gagaacgccg ggaattgaac ggttgtattc agccgtgaca aacaaaacgc cgtgggcatt
     2761 tttgatctcg tttttcaatc gctttacagc ttcagcctga tgggcatcat catcctgatt
     2821 gtataacggc aaatcgctga ttcgcgcttg ccggaaagag aaatccggcg gcgccaactt
     2881 cacaatagca tcagccagcc tgcgattgaa tgaatcttga cgaagacttc cgacaacaac
     2941 agcgatctga taatgactca tgacatcctc ccggcgtata aagagataac ttattgttta
     3001 tgctttgtaa tttattgaag ttcatcttac agtatttcgc atgcaaagaa catctaaaat
     3061 atctttttag gaaaagttta aaaaaataaa tggacacatc tatagattct ctgtcttcca
     3121 catttcgttt aaccggtttg aacattccat tcaagaaaag atgaaataaa cgcttcttca
     3181 tgatttctcg tcggcaacaa atgtggcaaa atcatccaat aaatcaagca gatataaaaa
     3241 ttatagatct attctgaaaa attggcacgg tattttcatt aatataaagg caaataaaac
     3301 aaattaccca aaggaggtaa caggtcatga agaaaacatt aggaaccatc atcgtagcgg
     3361 cagcggtagt tcttttaacg gcaaccttca gctttgctga atatgcggca gcaggtgcag
     3421 ccaatttccc cttcttccaa ctgggctgtt tagtggttgg cgggttgatt ctggtttccc
     3481 tgaaacggaa gtatgaaaag atgtacattg gtgaagttgt cggcgtcttc gctctctaca
     3541 cgattctgat ggcgctgttc accaacccgg ttattgaagt tgtaaaaaca atcgttactt
     3601 aacagacgca acaaaaacga acaagaggta cagtaacacg gcaagaaagt aaccgctacg
     3661 acaaacacat gatctgtttg ttatacgccc cctcaaggct ggcagagctt gagggggttt
     3721 ttatttcaaa aggagatggg gtgatatgaa atccgatcct ttcatcttcc atatcgtcaa
     3781 tcaccttttc atcaatccgc cgcatggttt ttcaatacca taacggcagt aacagaaaaa
     3841 gttcaggttg caattaaggc attgcggcaa ttcgacctga gcgatgttta cgtgcaaccg
     3901 gctatcagag cattcctgga aggatagccg atgatgaagg cagccaagtc aaaaagacct
     3961 ggctgaacag gacgccacag gctcattaga tggacacgac gattgacggc cgccttgcaa
     4021 taagagcagc agaaaaacat gaagaaaaaa cctcatgctt ttttgaatcc atattatctt
     4081 tccggccatc aaaaataatt gctgttgacg gatggcggat aagtagatta cacttcataa
     4141 aaattcactg gattgaaaaa gaggccatat tgaaaaaagc attcctccaa tcgaatagag
     4201 tcgtgtggcg ggtgattttg cccggtcaat gcaggtcatt gctgttggtt gtcctgatcg
     4261 ttcaatcatt tttattgtgc agttgcgcat cgactccttc cgaatcgttt ctcaaagaag
     4321 ccgcgattta tagttgctat ggcgggggaa gctacgcgga tacagtgctg aatgaatatg
     4381 ataaaaatac ggtcaggctg gaaaaattta aggttacgga aaagtacgac aagaaaatcg
     4441 atgatgaaaa ttatacgatt tacaagtatt tgataagcgt tcacgacaca aaaacccagt
     4501 tgacggacaa cctgtcgtgt tcagcggctg tcattaaacg cgggcctaaa tggtattacc
     4561 ggttgccgta gccggatctg cggtaaaatt tttactcttg acattttttt tagctggttc
     4621 aattgaagaa tgattgggcc ggcttttttt ataaggcaac tgacccatcg gacagtttat
     4681 gtaatgcaaa caggacaatt tatgcgcttc ttgcaggcgg tattctttcg ctggtaaaaa
     4741 aatgtatttt catttataat atattcattc acaaataagc ttcaatatca tcaaatagta
     4801 aaacgcatat ttgcaacgaa acaaccaacg cttcagataa tcaatacata gtcgttaatt
     4861 ggttttttca acccgaaata taattattaa acaaatatta taaaaaggag ggaaaaacat
     4921 gaattcaaat ttgacaacga tgtggaatgc catgcagagt ggcatgggaa atcacattcc
     4981 ggcccttttg ggagcattgg ctgttctggt tctggggtgg ttgcttgccg tattggcacg
     5041 ggcgggcgtc cgccggggac tgggcaagct taatttgaac acgcgtttcg ctcaacttac
     5101 aggtcagagc atcaaccttg aaaatggctt ggcaatagct attttttgga tcgttatgct
     5161 ttttatcata acggccgtgt taaactcgct tgatctggcg atggtttccg ccccctttgc
     5221 cgcgctcttg acacaatttc tggcatacat tccgcatctg ctggccgggt tgatgcttgc
     5281 tctgattgcc tggttactgg caagcatcgt acgcaggttc gtcaccaagg cgctttccaa
     5341 aaccacactg gatgaaaaac tttccgaaca tgcgggcatg tcgcccatca gtgacggact
     5401 gagtcaagcc ttgttctggc tcatcatcct gcttttcgta ccggccatta tgggcgcact
     5461 gcaaatggac ggtcttctgg atccgtttcg ggatatgatt accaaagtgc ttgattttct
     5521 gcccaacgcg gcagccgccg ttgccattgg cttcgtgggt tggattttag ccactgttct
     5581 gcgtaatctg acaaccaatc ttttgcgtac cgccggaagt gacaaactca gtcagaaggt
     5641 cgggttggcg gaatccgtta agctttccaa cgcgataggc ctgctggttt tcgtggcggt
     5701 gtttttaccg gcgctgatcg ccgctctgga cgcattaaaa atagaagcga tttccaaacc
     5761 ggcaatggat atgttggaca tgattttgat ggctgtgcct catatcgttg cggccggtct
     5821 tattctggtg atcacctgga tggttgcttc ttttgcttcc cggcttttgg ctagtctcct
     5881 caatgcaatg ggctttgaca acattccgaa acacatcgca ttggaacatg cgtttcaaaa
     5941 aacaccggca tcggtttttg tgggatacgt ggtcttgttt tttgccatgc ttttcgccac
     6001 ggttgaagcc gcaaatcagc tcggattcac gcacgtcagc gatacggtgg atacattcat
     6061 ccgcttcggc ggcgatgtgc ttttgggatc gcttattctt gtgataggat tctggctggc
     6121 caaccttgcc tatgaagcca tggacaaagc cagcggaccc aacacaaagg ggctggcgcg
     6181 aatcggacgt ttcgccattt tgggtctggt tttggcgctt ggtctgcgtg ctatgggcat
     6241 tgccgatgat attgtcaacc tcgcgttcgg attgacgttg ggcgccattg cggtgaccgt
     6301 ggcgttatcc ttcggtctgg gcggacgtga accggcaggg aaactcatgg aacactggct
     6361 gtcgaggttc cgtcaataaa gacggaaaat gatgggagca ccataaaaca agggcttttt
     6421 tgaaatggtg cagcctctgc cgaggctgca ccatttgttg ttgtctgtaa tttccttctg
     6481 ctggtggatt ttatttagat ggaatggcag cggaagggga atgtgcgcca ggcgctcatt
     6541 caggcgggga ccgtgcgctt acggccggtt atcctgaccg gtgccgccgt tgaggacagt
     6601 ttcgcagccg tttagagaaa aggattagca acgcaaaggc aaataaatcg cagtgcgtgc
     6661 agggatggca gctcagaatt ttagacggcg gggccgttga cagataaaaa ggctttacgt
     6721 atactgggca acaaaataat gtgaaagtat tttgaatgcg ttcgaacagg ttgccgaatc
     6781 ataacacaca ctgctcacgc tgttatcagc gtcgggaaat ctgcctgtgt cccatcatcc
     6841 ccgcggtgga aaccagcgtg gaatttctga tcctccggca tatttacgaa gcggaaaagc
     6901 ccagcaacac aggccgtctg gctgcgctgg cgcttacgaa ttcaaaactc atttcctgcg
     6961 gaggaggtca gcgcatcggt ctggcgtgcg tggatgacga tctgctgaat gctcccggaa
     7021 cctggctgtt gtggccggac ggagccggca cgcttccgcg gacatccgac aggcaatccc
     7081 cggctcgggt ggtggtgatt gatgcaacat ggcaacaggc ccgccggttg tacagcagtc
     7141 tgcccgcttt atggcggatg cctcgcctgg ccctggaggc gcctgtcgcc caaagaaaga
     7201 gattgcgcga tcagcaccgt ccgaattata tgtccaccct ggaggcggtg gcggctgcgg
     7261 tagcgatgct tgagggaggc gggaaaaatg tgccgctgga taatttatac gatgaattgg
     7321 ttcgccgaag acgttccttg cgatggggga gcaaagaatc ctgacgtttt tttgccgata
     7381 tagccggatc aggcagcaaa ggttccggtc ttcctgcccg aaattcacaa tagaatacaa
     7441 gccgtcaagg aaaatgaatc atgaaagaca taacaaaact tcgcatcata ctttattcag
     7501 ccatcgccgt gtttatcatg ctttgcgtta ttggatattt tttttcacgt taaatcggag
     7561 cgtcacgtcg ggtgcaaagc acccctccgt gtaatttcga gaaacgcagt ggcgagaaat
     7621 ctttaagact tcttaaattc gttcgaagta acacaacacc tccgagtcat accggcgcgg
     7681 gcctggccgg tatacaggaa aaaagaagac tggatgcccg acaagcccac tcgggcatga
     7741 caaacggagg gatgtccgac aaaattattt agttgatagt tgatagttaa tggttgatgg
     7801 ttctttgtgt catttcggtg atcaaaggat gtttgaaatg aaaaatggaa aacggtcaaa
     7861 atggatgtaa tgataagaag gatggccgag ttcagggagt tataaattgt tggaaaataa
     7921 aaattgtaaa aataaacgtg ttcatgtcgt tttgtttgat tttggcggcg tgctctcgga
     7981 agagggttgg aaaaaagggc ttagaatcat cgcaaaagcg aacggacttc atcccgacag
     8041 tttccttcaa acagccgccg atacgattta tgaaaccggt tatattctgg gaaaaggctc
     8101 gcaaagcgat ttctggaaat ctctcaaaac gaaaaccggc atccgggggg atgtcgcttc
     8161 gttatcgggt gaactgattt cgaggtttgc gctcaatgat gccatgatgg agactgtccg
     8221 gaatctgaag tcaaacaatt ttattgtcgg cattctgagc gatcagacgg actggctgga
     8281 aggactgaat gcacgttttg atttcttcag gcatttcgat tatgtcttta acagttatca
     8341 tctcggcaaa gggaaaagag atccgtcgct ttttgatgat attgccggct tactgagaga
     8401 aacaccggag aacattttat ttattgatga cgatccgggc aatattcaac gtgcccgaca
     8461 aaaaggatgg aaggcgattc tttacacgga ttttaaatca ttccgggagg agattaatga
     8521 aatcatacag gaaagaactc tggtttaata ccgcgcacag acgcgagttt atcaacataa
     8581 caggtcaggt ggaggattgt ctgcgcgaga gcgggattcg cgaaggactg gccttgatta
     8641 atacaacgaa agagtacttt tagcaaaagt tattcctttt acatttaagg gcgaagtcac
     8701 ggctctgcat tcatttcgta ttaaagaagc ctttggctat taatggccaa aggcttttct
     8761 gtttttggag atgagaatga aaaataaaat aataggcaaa aatggtgttc aaatagtgtc
     8821 attgaaccga cgaaaggcca ttcacgaaaa gtaccttaac tgttctgcat ggagcccaaa
     8881 agatgttgcc ggctgcaaat gaattcttgc cctctttatt catatcggac gagtaagggg
     8941 aagcagaatg tcaaagaaag acaaaagtcg attcgcacat attgcgtcgc ttgcatgaac
     9001 ggtcatgtgg gagcagtttt aagctgccag tcgtcggatt gtccactttt tatttatcgc
     9061 aaaggaggtg ttgataaatt ggcaagaatt tttatgaaga aaaattttaa tcacgaagca
     9121 gaacctttat taagccatga gtcacgaata tgagtgaagc ttttacgaga gagtcaatcc
     9181 gggagcatca ccgtgtaaag ataaaaaaga ttatcaatca aggtggcggt gttgaagaaa
     9241 ttgacaacta tctaatgaac agaatctcga cagtcattag ctatgaacgt cggaaaaatg
     9301 aacgcaggca actgcgcaag gtgacaaaaa accggtctgt tttttccaat gatgaggcgc
     9361 tgaccgaaat cttatatctg gcgattcggg atgtcatgaa aaaatgaacg atgcctttgg
     9421 ccaactgggc tttgacaatt tctcagctgg ctgttatgta cgagggccgt tttgatttgg
     9481 cggcaatatg aaaaattatc catttacaca gattagttta cagtctcatt ttgaaaattg
     9541 tgctgccttt caattcgtaa tcaataaagt tgcaccccat atcttgtggc agaatattct
     9601 tgactctcaa gaaaccgttt cctatactgc cgacagtaaa aaggaagttc taatcaattc
     9661 aatgacggtg tataaaagaa gactatggct gctgtgtctt gtcacaggta tcgtgttcca
     9721 ttgcgttcat gcccatgctc tggagccaac atttctaaaa gattccccaa aagtaaacat
     9781 ctgccgggct cttgaatttt tggaagatcc tgccggagaa ctgtcgttgg agcaacttct
     9841 tcacccgggc acgcaggatc gttttgtccc gttaaatgac ccgcagttaa atttcggtat
     9901 cacccgatcc acgtactggt tcaggtttct gctgaagttc gatcaatccg aaaattcatg
     9961 cgaatatgag tgcctggata acaattcatg gctcctggaa attgaccgtg cctggatgcc
    10021 gacaatcgag ctgtgggttc cggttatccg ggatagccat gatattcgga aaacggcaca
    10081 aatcatggga gaaagctgga gaaaactttc tgccgttaca tctgatcaat caaaccggat
    10141 aaacatctta aaccggagtt ccatattttt tatccccaaa gattatgatg aaagtcgtcc
    10201 catgtatctt aaaatggcgt cctggggacc gccgctgaat ttttcgctgc atgttagacg
    10261 attaaatgaa ttccgcaact ggcttacgtg ggatttcacc gtttttggca ttatttatgg
    10321 attgattatt gccatgaccc tttataatgc atgcatgtat ttgtccctcc gggacaagac
    10381 atatctggtg tatgtattat acatattttc aaacttcctc tacctgtcca tagggctcgg
    10441 tcatttcagt gcgcttaccg ggatactgtc gggatcacat ttttatctta tttacgtatt
    10501 caccggttct gctggttttt tcgccgcctg gttctgccgt acctttctgc tgacttccaa
    10561 gaatgcgccg cttattgata aaatattaat ggcgtatatg ttttttgcta tcctcttgat
    10621 attaatggct gttacgggtt tttattatca ggtgcagatt ttaggcgccg ccatcggctt
    10681 aacctttcca atcatggcta tcaccgccgg cctggtatgt tttatcaaag gcatgaagcc
    10741 ggccggatat tttttaactg catggtcggt cttatccgtc ggtatgctga cctgggcgtt
    10801 tcgcagcttg ggcatcctgc cccaaacaca gttaattacc tattcttatc cgatcgcggt
    10861 tgccgcggaa tccattttac tctccctggc cttggcggac cggataagaa cgttgcagca
    10921 ggaaaaccgg gaactggaaa acagggaaca ccgtctgttg attttaagca ataccgacgg
    10981 ccttacaaaa ttgtacaata aacggtattt taatgaaaaa ctaaaaagtg aaatctccct
    11041 ttccggtaat ttaggcgatc ccctgtcatt gttgatactg gatctggatg atttcaaaaa
    11101 gataaatgac gcgtacggac accctgccgg ggatcaggtg ctgaaacgac tgggcgaaat
    11161 ccttctgatg tcgattcgcg ataaggatta cggttgcaga tacggcggag aggagttcac
    11221 cgtcatcctg ccgggagccg aaaaggaaaa agccttggat gttgcggaaa gaatccgccg
    11281 cttaatcagt gatgatactt ttgaaacaag cgacggacaa aaattttcag tgactgtcag
    11341 catcggtctt gcccagctcc gtcccggtga aaacagcgcg gatttaatta atagagccga
    11401 ccagtcactc tatagtgcca aacgccaggg caaaaaccaa acagttgcat aacaaccgct
    11461 tatttgtgag taccgggaat tctggggaca aggcgttaaa atgaaattcc tgcgatatat
    11521 atgggcactg cccaacacga cgctgggact tctgtttgtt ccgctggctt tgtttacccg
    11581 tggcgggatg catgtggtta acggtgtgct cgagctacac agcaggctca tatcgctcat
    11641 ccttaaaaaa tgtatgcctg tacgtggcgg cgttagcgcg ataaccctgg gacaaaacag
    11701 agaatctctt tccatcaatc gtcggcatga gcatgcgcat gtgcatgtgc ggcaatatga
    11761 aatgttaggg ccgatattta ttccggtctc tgaaaaactg atttcctggc tgactgaaaa
    11821 ccaaatcaaa gtcctcaatg ttgcggggcc aagattacgt aaagacccaa cgatatatga
    11881 tgctgtattg cggctgttaa aggtaaccct ttcagctgct taatggaggc aaagaatgaa
    11941 gtcgttcaga aaagaacttt ggttcaatac caagaaaaga agggaattta tcaatatcac
    12001 ttcacaggtg gaagattgtt tagaggagag tggcattcat gaaggtatcg tgttagtcaa
    12061 cgccatgcat atcacggcgt cggtgttcat caacgatgat gaatccggtc tgcaccagga
    12121 ctacgaaaac tggctggaaa acctggcgcc ccatgagccc gttgc
//
LOCUS       DAONQZ010000068        11988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5894_length_11988_cov_23.902455, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000068 DAONQZ010000000
VERSION     DAONQZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5894_length_11988_cov_23.902455"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..683)
                     /gene="cas2"
                     /locus_tag="PLR13_09535"
     CDS             complement(393..683)
                     /gene="cas2"
                     /locus_tag="PLR13_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQC19622.1"
                     /translation="MLVLVSYDVAMNDERGPKRLRRVAKTCQNYGQRVQYSVFECIVD
                     PAQWTVLREQLIKEINQEEDSLRFYFLGSNWRRRVEHVGAKKTIDQEGPLIV"
     gene            complement(695..1726)
                     /gene="cas1c"
                     /locus_tag="PLR13_09540"
     CDS             complement(695..1726)
                     /gene="cas1c"
                     /locus_tag="PLR13_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HQC19623.1"
                     /translation="MKKLLNTLYVTTQGAYLAKEGETVVVKIDQEVRLRVPVHTIGGI
                     VCFGNVSCSPFLMGFCGENGVSISFLSEYGRFLAWVHGPVSGNVLLRRAQYRLADDQE
                     YSAQMAKYFVTGKIANCRTILRRALRDHSEKMNDDALSQGVSTLSSSLKSLELNQTLD
                     AVRGLEGDAAHTYFAVFDNLIIAQKESFAFQERNRRPPLDNVNCLLSFLYTLLVHDCR
                     SALEAVGLDPAVGFLHRDRPGRPSLALDLMEEFRPFLADRLALSLINLKQVQDKGFSK
                     TEAGAVMMNDETRKTLLVAYQERKREEIYHPFIDEKVNIGLLFHVQAMLLARCLRGDL
                     DGYPPFIWK"
     gene            complement(1710..2354)
                     /gene="cas4"
                     /locus_tag="PLR13_09545"
     CDS             complement(1710..2354)
                     /gene="cas4"
                     /locus_tag="PLR13_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HQC19624.1"
                     /translation="MYSEEDLIQLSALQHYAFCPRQCALIHVEQVWSENLLTAEGRIM
                     HEHVHEEGDESRGDVRIERGVALRSLNLGLIGKADVIEYHRQADGTWQAFPVEYKRGK
                     PKPDDSDKVQLCAQAMCLEEMLNVRIPAGALFYGKTRRRLDVNIDDALRRETQEAAQK
                     THELIESGQTPKPAYAKRCESCSLVAECMPKTIQKKKSVESYLKRMLDDYEKAS"
     gene            complement(2344..3291)
                     /gene="cas7c"
                     /locus_tag="PLR13_09550"
     CDS             complement(2344..3291)
                     /gene="cas7c"
                     /locus_tag="PLR13_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HQC19625.1"
                     /translation="MSNPIKNRYDFVFFFDVKDGNPNGDPDYVNLPRADAEDQHGLVT
                     DVCLKRKVRNYVQLKYNLSAPYNIFIRQRDINEEDSYLNAVIGKAKGNSASEKKKYLC
                     EDYYDIRTFGAVLTTGEKENAEPSEEDEDGKKPKKQKKENVRGAGTVRGPVQFTFARS
                     EDRIYQAEHSITRCCVATEKEKSDQAKKDREYATTFGRKSTVPYALYRMHGFISAADA
                     EKAKFSEADLKLLWESLINAFENDRAAARGEMNPRKLVIFKHDNHLGNELAGRLFERV
                     TVKKNSELPRGISDYSILVNKDKLPSGITLQEWPEENVF"
     gene            complement(3308..5140)
                     /gene="cas8c"
                     /locus_tag="PLR13_09555"
     CDS             complement(3308..5140)
                     /gene="cas8c"
                     /locus_tag="PLR13_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HQC19626.1"
                     /translation="MIKELSDLGKKLRSQKADDEWVHDALKSEVISLVLTIKPDGSFI
                     SLHPIERKKTTAEAVQRTSGKGARLLMDNIGYVLGVFDPESTAFKKKAKEKGELKAKE
                     IFIKDVSNKNKLFIERLDDLKELDELNPVIKFYGNNKKQGIDLVDQDYFTKNISKKDR
                     SGNIAILVSGRDKYVHEYATVYEKIIELYNKRQDKFLSADEISCSVCSKNDFPIGDFT
                     HYPIKGVPGDKEPAGGRKLISYNVTAFESYALRGNENSSICTNCAKTYVEGLNWLLSS
                     GNEIVTTTKKGKDKKSYRYTNRKNFGSDTAIVFWTRENKDLPEIDQLEAPNPDDVNKL
                     LESLSSGREQDGRYLEADQFYSCALSGSAARIAVRDWIESSLFDFRKSIARWFKDIAI
                     MEYDSTAKTLTTRYFGLYNLARSSQNVTNDKDVSLSRTAAYLWNAAIKQTAPPRWLLT
                     KVLQRARMDEYGVTAERASLIKLILNRNNKGGGFMVAEKIEDGFKPVAYVCGQIFAKL
                     ESIQYAALGDRNAGIRERYFTYAMTSPGAAFGRLFNLHSKHFTKLKNEKPGLAINMDK
                     ELQELCKDVDIKNFPATFSLEEQGQFAIGYYHQRQKQFNTSEKN"
     gene            complement(5137..5880)
                     /gene="cas5c"
                     /locus_tag="PLR13_09560"
     CDS             complement(5137..5880)
                     /gene="cas5c"
                     /locus_tag="PLR13_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HQC19627.1"
                     /translation="MLDSRIVRVKVTGGYACFTRPDLKVERMTYPCMTPSAARGILDS
                     ILWKPEFQWYVRKIIVLNPVRFFTIKRNEINTKQGRTPIIIEDKRAQRNSVVLRDVAY
                     IIEASIYQKDSSARNKPEKYIGRKGVDADHDGIFIRRVKKGQCWRRPYLGTREFPAEF
                     MEPHGKEEPLKETIPIGSMLFDIFYSEKGKPEPIFFYDVAVRNGVLNCEVPENDKMMQ
                     SSHIQPPIDSEISSAIYAFNQQEEKEATL"
     gene            complement(5894..8071)
                     /gene="cas3"
                     /locus_tag="PLR13_09565"
     CDS             complement(5894..8071)
                     /gene="cas3"
                     /locus_tag="PLR13_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HQC19628.1"
                     /translation="MEYIAHSENSLHETHSLSKHLHETAKLSSSFAWKEEYKPIFYLA
                     GLLHDLGKYQAAFQEYLRSGGKRGSVPHASWGAGFTRIKHMLEMSFVIDGHHKGIPDN
                     STWKFDTEPFKRNEKEEYEAVIARFLEDNQIQEGELYKKLAEEISKTSSHREIFIRYL
                     FSSLTDSDWLSTEKHFDKGISEIRSDISLPIEEMIQKLEAGLSKKPKEGKINQLRNAA
                     RYEALEKANQPCGFYSLSLPTGMGKTLTSVVWALRHAKANTLKRIIIVLPFVNIIDQT
                     AQILKDIFGNELVLEHHSNFNEDDTANDLDEANYSLLEQRKKLACENWNYPIIVTTTV
                     QFFESLFSNRPSKCRKIHNIAESVIIFDEVQTLPKEVILPTLQMLKDVQAIMKTSFLF
                     CTATQPSFEKRNGFDGIENIQPLITDSAEMYKETKRVEYKLLNDLKPIDLSDLLKATS
                     DEGASALVIFNTKKPTFDFFNLAKNDSRWENKYHLSTGMCPDHRKRVIRNIRDDLTAK
                     RKILVSSTQLIEAGVDFDFPVVFRALAPLEAIIQAAGRCNRENNLGPSGGKVFLFKMS
                     DSAMPDKTYAACANHAEEFIKPDIEQLHNPDTFNRYYAQVVNLYVDPDQYKINQARKD
                     FNFHTVNDSYRIIRDSTEGLYMYHYSDESRQLFHSIEHKEFLSKDDYRKMQMFTVSVY
                     RNFIFKNSANCKVMPQGFMVWYGNYDQQTGISIEPLEADKLIV"
     gene            complement(8226..8654)
                     /locus_tag="PLR13_09570"
     CDS             complement(8226..8654)
                     /locus_tag="PLR13_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19629.1"
                     /translation="MVKDQHYRYPRVNRAHFKPNFVKKDRDDQIDLGWAEGVFSDGRP
                     FRLEVWAATYVGYYTYYFSIVDIEHYSEIDLKRYLESEDVIEFDDEKYLASGFTGINV
                     SARKVADDSGNDIWEVTIIVDDDDGPYVHNKILTCGYREE"
     gene            complement(10632..11225)
                     /locus_tag="PLR13_09575"
     CDS             complement(10632..11225)
                     /locus_tag="PLR13_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQC19630.1"
                     /translation="MVKGQNFNRPDPGSKIKVQPIKTAKDIETIKKLLKDNPLHMAIF
                     TLGINTNLRASDLLSIKVSQVRGLKPMSEIDITERKTGKSRKINLNKKCVDAINELLA
                     SRTYQEDDYLFIGQRGRLTVPYLNNLVKSWCAKINLKGNYGSHTLRKTFGHIQHVNFG
                     VPLPELMVCYNHATQKQTLDYLCIQPDEIRKVYANEI"
     gene            complement(11300..11977)
                     /locus_tag="PLR13_09580"
     CDS             complement(11300..11977)
                     /locus_tag="PLR13_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19631.1"
                     /translation="MKKVMIYVVAFLCFLVSACNDDIAVPADLHTRLKLNFTNGLADS
                     SIYSNKVVAVDGVAVTAGVAVFTGGWLNVQDSPLLDLSDKNFTIKFRLHPSSTSIRGI
                     IGKFVDRDNYWMIYADGPAKTINFEAKERSVTIAQYAFQVPYQTGRWRSYSLSREKEN
                     LYMVCENKRYEPIITVPIYSAAIPNFLTAAGQYPIGIGRPAGIPITAAGVRPWAGMMD
                     EICIIVF"
BASE COUNT         3264 a         2672 c         2219 g         3833 t
ORIGIN      
        1 aatccacgcg cccatgcggg gcgcgacttt gttactgttg atagcaagcg ttaccgtgta
       61 atgtttcaat ccacgcgccc atgcggggcg cgacgtctaa ctttgttgtt tgttacccag
      121 tatgatgtgt ttcaatccac gcgcccatgc ggggcgcgac tctttaaatg taaccactta
      181 taaaaattta tctttctcct tatcatccgc gagctattcg ttttacttgt attctctatt
      241 caattttcaa agagcatttt ataaaataaa taatatttca ataaattgac tcatccgcga
      301 accttattaa taaatattgt cagcttctgg ttcgcggcat tttttattag gacaaaaaat
      361 tttccccttt cgatcataat atccaaaaaa tactagacga taagcggccc ttcctgatca
      421 atcgttttct tcgcgccaac atgttccaca cgcctgcgcc agttggaccc aagaaaataa
      481 aatcgcaggc tgtcttcctc ctgatttatt tctttaatca gttgttctct caaaacagtc
      541 cattgcgccg gatcaacgat acattcaaaa acagagtatt gcacccgctg cccgtaattt
      601 tgacaggtct tggcaacccg ccttagacgt tttgggccac gttcatcatt catcgccaca
      661 tcataactga ccagcacaag cattttttat ctccttactt ccagataaat ggtggatagc
      721 cgtccagatc accgcgcaag catcgagcca gaagcatggc ctgcacatga aacagcaaac
      781 cgatatttac tttttcatca atgaaaggat gatatatttc ctcccgcttt ctttcctgat
      841 aagccacaag taacgttttc cttgtttcat cattcatcat aacggcgcct gcttctgttt
      901 tgctgaatcc tttatcctgt acctgtttta aattaattaa agacaacgcc aaacgatcag
      961 caagaaacgg acggaattct tccatcaaat ccagagccaa actgggtctg cccggtctgt
     1021 cccggtgcaa aaaaccaacc gccggatcaa gaccgacggc ttccaacgcc gaacggcaat
     1081 catgcaccag caatgtataa agaaaggaca aaagacaatt tacattatcc agtggcggcc
     1141 ggcgattgcg ttcctgaaac gcaaagctct ctttctgcgc aataatcaaa ttatcaaaaa
     1201 cggcaaaata agtatgcgcg gcatcgcctt ccagcccgcg aacggcatca agcgtttgat
     1261 tcaattccaa cgacttcagt gaactgctta acgtagaaac accttgactc agtgcatcat
     1321 cattcatctt ttcagaatga tctcgcagcg cacgtcgcag tattgttcgg caattggcaa
     1381 ttttacccgt cacaaaatac ttcgccattt gtgcagagta ttcctgatca tcggcaagcc
     1441 gatattgcgc acgtcgcaac aatacattac cggataccgg accatgcacc catgcaagaa
     1501 atctgccata ttccgataag aagctgatgc taacaccgtt ttcaccgcaa aagcccatca
     1561 gaaacggact gcaagacaca ttgccgaaac agacaatgcc accaatcgta tgcaccggca
     1621 cacgcaggcg aacttcctga tcaatcttga ccaccactgt ttcgccttcc ttggcaaggt
     1681 acgcgccttg tgtcgtcaca tacaaagtat taagaagctt tttcataatc atccaacatc
     1741 cgttttaaat aactttctac cgactttttt ttctgtatcg ttttaggcat acactcagcc
     1801 accagcgaac agctttcaca gcgcttggca taagcaggct ttggtgtttg gcctgattcg
     1861 attaattcat gggttttttg cgctgcctcc tgcgtttccc gccgcaatgc atcatcaatg
     1921 ttaacatcaa gacgtcttct ggttttgcca tagaatagcg caccagccgg aatgcgaaca
     1981 ttgagcattt cttccagaca catcgcctgt gcacaaagtt gcactttgtc gctatcatca
     2041 ggtttcggtt taccccgttt gtattcaacc ggaaaagcct gccaagttcc atcggcctgt
     2101 cggtgatatt caatcacatc cgccttgccg attaaaccga gattcaaaga acgcagagca
     2161 acgccgcgct caatgcgtac atcaccgcgt gattcatcgc cttcttcatg cacatgctcg
     2221 tgcataatcc tgccttcggc ggtcaggaga ttctcagacc acacctgctc gacatgaatc
     2281 aacgcacact gccttggaca aaaggcataa tgctgcaagg ctgaaagctg gattaaatct
     2341 tcttcagaat acattttctt ccggccattc ctgtaatgtt atcccggaag gtagtttgtc
     2401 tttattaacg agtatcgaat aatcacttat tccacgagga agctctgaat ttttctttac
     2461 ggttactctc tcgaacaatc tgccagcaag ttcatttccg agatggttgt catgtttaaa
     2521 aataactaat tttcgcggat tcatttcgcc acgcgcggcg gcacgatcgt tttcaaaggc
     2581 atttattaga gattcccaaa gaagttttaa atcagcctca gaaaactttg ctttttcagc
     2641 atcagcagcg gagataaaac catgcattct ataaagtgca taaggcacgg ttgattttct
     2701 gccaaaagta gtcgcatatt cccggtcctt ttttgcctga tcacttttct ctttttctgt
     2761 ggcaacacaa catcgcgtta ttgaatgctc ggcctgataa attctatctt ctgaacgagc
     2821 aaaagtgaat tgcactggac cacgaacagt accggcaccc cgtacatttt ctttcttttg
     2881 ctttttcggc tttttcccat cttcatcttc ttctgacggt tcagcattct ctttctcgcc
     2941 ggttgtcaaa acagcaccga atgttctgat atcataatag tcttcacata aatatttttt
     3001 cttttccgac gcactattgc cttttgcttt tccgattact gcattcagat agctatcttc
     3061 ttcgtttata tctctttgcc tgataaaaat attatacggt gctgacaggt tatactttaa
     3121 ttggacatag tttctaactt ttcttttaag acaaacatcc gttaccaaac cgtgttgatc
     3181 ttcagcatcc gctcgcggca aattaacata atctgggtcg ccattaggat tgccatcttt
     3241 aacatcaaaa aagaatacaa aatcatatct gttttttatc gggttactca tgtcattcct
     3301 cctctaatta atttttttcg gatgtattga attgtttttg gcgctgatga taatagccaa
     3361 tggcaaactg tccttgctct tcaagagaaa atgttgccgg gaaatttttg atatcaacgt
     3421 ccttgcaaag ttcttgaagc tctttatcca tattgattgc aaggcctggt ttttcgttct
     3481 tcaactttgt gaaatgcttg gaatgcaggt tgaaaagacg accaaacgca gcaccaggcg
     3541 aagtcatggc ataggtaaag tacctttccc gaatgcctgc attgcgatca cccaaagcgg
     3601 catactgaat actttccagt tttgcaaaga tttgcccgca gacatacgca acaggcttga
     3661 aaccatcttc aattttttct gcgaccataa aaccacctcc tttattattt ctatttaaaa
     3721 tgagttttat cagggatgca cgttccgctg ttacaccata ttcatccata cgtgcgcgct
     3781 gcagcacctt tgtcaataac cagcgtggcg gcgctgtctg tttgattgcc gcattccata
     3841 agtaggcggc tgttcttgac aatgaaacat ccttgtcatt tgtgacgttt tgagagcttc
     3901 gcgccaggtt atataagccg aaatacctcg ttgttaatgt ctttgctgtg ctgtcgtatt
     3961 ccatgatggc aatatcttta aaccatctgg ctatagattt ccggaaatcg aacaaacttg
     4021 attcaatcca atcgcgaacg gcaatacgag ctgccgaacc ggataatgca caggaataaa
     4081 actgatctgc ttccaaatat ctgccgtctt gttctcttcc cgaagacaac gattccagca
     4141 atttattcac atcatcagga ttgggggctt ccagttgatc aatttcgggc aaatccttat
     4201 tttcccgtgt ccaaaataca atagcagtat ctgacccgaa gtttttacga tttgtgtaac
     4261 gatacgattt tttgtctttt ccttttttcg ttgtcgtgac aatttcattg cctgatgata
     4321 aaagccaatt caatccttca acatacgtct ttgcgcaatt tgtacatatt gaagagtttt
     4381 cattacctct taaagcatac gattcaaagg cagtaacgtt atatgaaatc agttttcgac
     4441 cacctgctgg ttctttatcg cctggcacac ctttaatagg gtaatgtgta aaatcgccaa
     4501 tcggaaaatc gtttttggaa cacacagaac aacttatttc atcggctgat aagaatttat
     4561 cttgtcgctt gttataaagc tcaatgattt tctcatatac tgtagcgtat tcgtgaacat
     4621 atttatctct gcctgaaact aatattgcta tatttccact tctatctttt ttagaaatgt
     4681 tcttggtgaa atagtcttga tcgactaaat caatcccttg ctttttatta tttccataaa
     4741 atttaataac tggatttagc tcatccaact ctttaagatc atctaagcgc tcaataaaaa
     4801 gcttattttt atttgaaaca tcttttataa agatctcttt agcttttaat tccccttttt
     4861 ctttagcctt ctttttaaag gctgtgcttt caggatcaaa gactcctaaa acatacccaa
     4921 tattatccat caataatcga gcacctttac cacttgtcct ttgtacagct tcagcagttg
     4981 tcttcttccg ttcaatagga tgtagagaga tgaaactacc atccggctta atcgttaata
     5041 ctaatgagat aacttcactc tttaatgcat catgaaccca ttcatcatca gccttctgcg
     5101 aacgtaactt tttccccaga tcgcttaact ccttaatcat aaggtggcct ccttttcttc
     5161 ctgctgattg aaagcgtaaa tagcggagga aatttcacta tcaatcggcg gttgaatatg
     5221 actggactgc atcattttgt cattttcagg aacttcacaa ttcaagacgc catttctcac
     5281 ggcaacatca tagaagaaaa taggttccgg tttccctttc tcactataga agatgtcgaa
     5341 caacatactt ccgatgggga tggtctcttt tagcggttct tctttaccgt gtggctccat
     5401 aaattctgct ggaaactcgc gggtaccgag ataggggcgc cgccagcatt gtcctttctt
     5461 tacacgtcga ataaaaatac cgtcgtgatc ggcatcaacg cctttcctgc caatatattt
     5521 ttcgggttta ttacgagccg acgaatcttt ctgatagatt gacgcttcaa taatgtatgc
     5581 cacatccctt aagaccacgc tgtttcgttg cgccctttta tcttcaatga ttatcggtgt
     5641 ccgtccttgc tttgtattga tctcattgcg tttaattgta aagaaacgca cgggattgag
     5701 aacaattatt tttctgacat accattggaa ttccggtttc catagtatgg aatcaagaat
     5761 gccgcgtgca gcagatggtg tcatgcacgg gtacgtcatt ctttcaacct tgaggtccgg
     5821 cctggtgaaa caagcataac cgcctgtgac cttgactctt actattctac tgtcaagcat
     5881 atttacctcc tgtttatacg attaatttat ccgcttctaa tggctctatt gagatgcccg
     5941 tttgctggtc gtaatttcca taccaaacca taaatccttg gggcatgact ttgcaatttg
     6001 cactattttt aaagatgaaa ttcctgtaaa cagaaactgt aaacatttgc attttcctgt
     6061 aatcatcctt tgataaaaat tccttatgct cgattgaatg aaataattgt ctgctttcat
     6121 cactgtaatg gtacatatac aatccttcag ttgaatccct gattatccgg taactgtcat
     6181 ttactgtatg aaagttgaaa tctttacgcg cctgattaat tttatactga tcaggatcaa
     6241 cataaagatt gaccacttgt gcataatacc tattgaaagt atccggatta tgcagttgtt
     6301 caatatccgg tttgataaat tcttcagcgt gatttgcaca tgcagcatat gttttgtcag
     6361 gcattgcact atcgctcatt ttaaatagaa atacttttcc accagaaggt cctaaattgt
     6421 tttctcgatt gcaacgacca gccgcttgaa taattgcttc aagcggtgca agggcacgaa
     6481 agaccacagg gaaatcaaaa tcaacgcctg cctcaatcaa ttgggttgag gagacaagaa
     6541 ttttacgttt ggccgtcaaa tcgtcccgta tattcctgat aactcgttta cgatgatctg
     6601 ggcacatacc cgttgatagg tgatatttgt tttcccaacg tgaatcattc tttgctaaat
     6661 taaaaaaatc aaacgtcggt ttttttgtgt tgaatattac caatgcagaa gcaccttcat
     6721 ccgacgttgc tttgagaaga tcagataaat ctataggttt aagatcattc agtaatttat
     6781 attcaacacg ctttgtttcc ttatacattt cagcggaatc ggtaatcaaa ggttggatgt
     6841 tttctatacc atcaaagcca tttctctttt caaatgacgg ttgtgttgcc gtacagaaca
     6901 ggaaagacgt tttcataatt gcttgaacat ctttaagcat ttgaagcgtc ggcaggatta
     6961 cctctttggg cagcgtctga acttcatcaa agataattac agattcagca atattgtgga
     7021 tttttctgca tttggacggt cggttactga aaagtgactc gaaaaattga actgttgttg
     7081 tcacgataat aggataattc cagttttcac aggccagctt ttttctttgc tctagaagag
     7141 agtaattagc ttcatcaaga tcgtttgctg tatcatcttc gttaaaattg gaatgatgtt
     7201 ccaataccaa ttcattaccg aaaatatcct tcaatatctg cgccgtttga tcaataatat
     7261 ttacaaaggg caggactatt atgattcgct tcaaagtatt tgcttttgcg tgccgtaacg
     7321 cccaaaccac cgatgtgagc gttttcccca taccagtggg caaagataaa gaataaaagc
     7381 cacatggttg attagctttt tcgagcgctt cataacgcgc ggcatttcgt aactgattaa
     7441 ttttcccttc tttaggcttt ttcgataatc ctgcttctaa tttttggatc atttcttcaa
     7501 tcggcagaga aatgtcagat cgaatttccg aaatgccttt gtcaaaatgt ttttcagtag
     7561 aaagccaatc actgtctgtc aaagaactaa ataggtatct aatgaaaatt tctctgtggg
     7621 acgaggtttt agatatttct tctgccagtt ttttatataa ttcaccctct tgtatttggt
     7681 tatcttcgag aaatcgggca atgaccgctt catattcttc tttttcattt cttttgaacg
     7741 gttcagtatc aaacttccat gttgaattat cgggaatacc tttgtgatga ccatcaatta
     7801 caaaagacat ttccagcatg tgtttaatac gtgtaaatcc agcaccccat gatgcgtgcg
     7861 gaacgctacc gcgtttacca ccacttctta aatattcctg aaaggctgcc tgatatttgc
     7921 caagatcgtg taataatccg gcaaggtaaa atattggttt atactcttct ttccaagcga
     7981 atgatgatga aagttttgca gtttcatgaa gatgcttaga taaagagtgt gtttcatgaa
     8041 gactgttttc tgaatgtgca atgtattcca taattattca aatttctctt ttgttgtttg
     8101 atgttattgt atttctattc ctattccacc cccatgtggt gtccgatggg ctttttattt
     8161 aaaaatattt ttattacgct ctggattccc tagtcacgta agcttacgta tacagcttag
     8221 cgtcattatt cttctctgta tccacaagtt aatatcttat tatgcacata cggtccgtca
     8281 tcatcatcta caataatcgt aacttcccaa atatcattac ccgaatcatc cgctactttt
     8341 cgggcgctaa cgtttatacc tgtaaacccc gaggccaaat atttttcatc atcaaattca
     8401 attacatctt ctgattccaa atatcttttt aaatctattt cggaataatg ttcaatatca
     8461 actatcgaaa agtagtaggt ataatatcca acgtatgttg ctgcccatac ctcgagccta
     8521 aacggtctac catccgaaaa tactccttcc gcccaaccta aatcaatctg gtcgtcacgg
     8581 tcttttttta caaaatttgg tttgaaatgt gccctgttta ctcttggata tctataatgc
     8641 tgatctttga ccataatttg ccgctccccc tgattttggg aaatgttatt tcttcgtcat
     8701 cattggaaac aactttttgc ggtgtttttc tctggaacgg tcgttttgaa aacagttttt
     8761 aattctcgct gtttttcctg ctatagtcat gcttttccat ttagtgtcaa gataacaccc
     8821 aatgaactca agttttgaaa tattatttct aactgtttcc agtaaggatt ctttatgttt
     8881 ggctgtttga attgctgtgc agtatagcct ccgctttcgg acgtgtcaat cgtaaaaaac
     8941 tttgggataa atacagacag aattattttt gattacaatg aatgttaata tcaaatcagg
     9001 cgcacccatc tctttggcga gtgtggtgtg cacaaacgaa gaagttcgca tttcgtgata
     9061 taactataac acgagttttg gaattttaat ggcgggggtg aaaactactt ttcaaaaccg
     9121 ttcacagtta ttcatagttg tccacagcgc gtggaaaaaa aacttaacaa gtatttttct
     9181 tcggtaagaa ctttttttac agagtcagac agaaaaatat ttttctcatt taacgcgaaa
     9241 aattatccat tagaagccaa aaatcatttc acttaagtca gggtgacgtt ttgaagagat
     9301 cgtttctctc agggcgaata aaagcgtttc ccgtgccccc aaattatttt tgatgattaa
     9361 gaagttttaa aaatcaaaat ttcgcataag caaagttatt gagtggggga gggagtccca
     9421 agtctccggg tttttgtttt cggggtgttg gttgtgtggt gactgcacag cgcggcggtt
     9481 agaacggcaa agaacaacgg cggcccgcgc gccagccgga caaaaaatta tggccagcaa
     9541 tagcgaatta taaatttgag atttttaatt gccaaactca ttaactattc cctaaaagtt
     9601 aaagagtata aaaacaacaa taattaaata atctcaatac tttatgttat tttttccgtt
     9661 ggcgcgatta atcctcttgc cttatctcat taactattta ttataataac atcattattt
     9721 aatcagtttc actggatcat ttcattcttt ttggcgcaat ccgcttcaaa aactattcaa
     9781 taagttaaat accaaatatt attatacttt cccacagaag cccccagccc ccggcaattc
     9841 cggataaaaa ccgctgaatg aggatgttgt ggaaatgaat attgtcagga aaatataaaa
     9901 tcaggatgat cctggtgaat aaaaactttt aacaattcgc ctacccatcg tcattttatt
     9961 cattcagaca ggttttacag gtttttccct attattaatt aatttattat tcactctata
    10021 cctatactag tctaaaccgc acgcgcccgc gcgatggaag gaaaatgtat atttatatat
    10081 aattaataga gccccaaaaa gctgtcaaac ctgtcatgta attaatatta ttaaagaaaa
    10141 caataaatca aaaacctgtc ggaaacctgt ctgaaagcca aaaagactgt cttttgatac
    10201 tcacgaacaa aatagaagag ggccgcgggt gcggagaaaa gccagggttt ggataaccgg
    10261 acagattttg agataagttt ttagtgggga ttagggtatg ccccaaaaga gggcaaaaaa
    10321 ggtatcgcca tccggcccag gattggccag atggcgatct ttaattttaa ttgcgctgct
    10381 gctgccaagc gtcggctttc attaattcga tgagtcgggc gcggattgtt gtgtttctca
    10441 tcacgcagac ggctttaaat tcgctgtgga tgcttttagg cacggtccgg ataagcattt
    10501 ttgcactgtg tcttctgcag attttattgg tgttttggtc attttatcat tccctcgcta
    10561 tttttgactg cttatattgc ctttagagcc gcgatctcgg cctgggcctt gtctcttgat
    10621 acctgccgtt tttagatttc attagcataa acttttctga tctcatccgg ctggatgcag
    10681 agatagtcca gcgtttgttt ttgcgtggcg tgattgtagc aaaccattaa ttcgggtaac
    10741 ggcacgccga aattaacatg ctggatatgg ccgaacgttt tgcgcagcgt atggcttcca
    10801 tagttgccct tgagattgat tttggcgcac catgatttta ccaaattgtt aagatagggc
    10861 acggttaagc ggccccgttg gccgatgaac aaataatcat cttcctggta tgtcctggat
    10921 gccagtaact cgttgatcgc gtccacgcat tttttattga gatttatttt tctggatttg
    10981 ccggttttgc gctcggtgat atcaatttcg ctcatcggtt tcaggccgcg gacctgactg
    11041 actttgatgc tcaataaatc ggatgcccga agatttgtgt tgatccccag ggtgaaaatt
    11101 gccatgtgca gcggattatc cttgagcagt tttttgattg tttcgatatc ctttgctgtt
    11161 ttgattggtt gcactttaat tttactgccc ggatccgggc ggttgaagtt ttgaccttta
    11221 accatgtctg cctccttgtg atattaaaat tagttaatgt atttcaggcc gggaatacct
    11281 ggtcccggcc tgattatccc taaaacacaa taatacaaat ttcatccatc atacctgccc
    11341 acggccggac gccagcggcc gtaataggaa tacccgcagg cctgcctata ccgattggat
    11401 attgcccggc ggccgttaaa aaattaggga tcgcggcgga atatatcggc acggttataa
    11461 tcggctcgta gcgtttgttt tcgcagacca tgtaaagatt ttctttttcc cggctcaagc
    11521 tataactacg ccatcttcca gtttggtagg gcacttggaa agcatactga gctatcgtca
    11581 cgctgcgctc tttcgcctca aagttaatag ttttcgccgg accatccgca tagatcatcc
    11641 agtaattatc ccgatcgaca aatttgccga ttatgccgcg tatgctggta gaagatggat
    11701 gcaaacgaaa ttttattgta aagtttttat cggacaaatc gagtaggggc gagtcctgca
    11761 cattgagcca gccgccggtg aacacggcca cgccagcagt aacagccacg ccatcaacag
    11821 caacaacttt attgctgtaa atagagctat ctgccagacc gttggtgaag tttaatttta
    11881 gtcgggtgtg aagatcggca ggcactgcga tatcatcatt acacgcgcta actaaaaaac
    11941 acaaaaacgc aacaacataa atcatcactt ttttcataac tcctcctt
//
LOCUS       DAONQZ010000069        11771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_5994_length_11771_cov_16.585353, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000069 DAONQZ010000000
VERSION     DAONQZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_11771_cov_16.585353"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1058
                     /gene="glyQ"
                     /locus_tag="PLR13_09585"
     CDS             171..1058
                     /gene="glyQ"
                     /locus_tag="PLR13_09585"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQC19632.1"
                     /translation="MTFQELIFALEKYWDSKGCVIQQPYDMEVGAGTFHPATCLRALG
                     PEPWNVAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPVNIQELYLDSLKSFGID
                     PLDHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGFDCSPVCAELTYG
                     TERIAMYIQGVNNVYDLQWTDKIKYGDVHHRGEVEFSTYNFEVADIPMLRKLFDTYEE
                     EALRIAEKNLALPAYDYCLKCSHTFNLLNARGAISVAERTSYIGRVRNLARISAELYM
                     KQREELGYPLLSRSAYSDL"
     gene            1157..3229
                     /gene="glyS"
                     /locus_tag="PLR13_09590"
     CDS             1157..3229
                     /gene="glyS"
                     /locus_tag="PLR13_09590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQC19633.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEIIRKSFTDKRIAFDGI
                     KCLATPRRLVLYIADLSAKQEDQTVEKLGPAKKAAFDENGQPTKAAIGFARGQGVDVS
                     QLETITTEKGEYLGARKTIAGQPTKDLLPDILKDFMLAIPFRKSMRWADCTLRFARPI
                     HWILALYSGEVIPLKIEDIESGNTSCGHRFMSPAPFAVAGFEDYLNKARENFVIADPA
                     ERRKLILEEAEKSAAEVGGKLFYTDDLLETVSFIVEYPVIVRGGYEEDYLKIPKDVLT
                     TTMISHQKYFPVVNEAGKLLPYFIAVSNTKPRDISVVAKGNERVLRARLADASFFFEE
                     DKKVPLEDRVESLKKVVFHTLLGTSHKKVARFRKLAIKIASKVKPSAKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALIAGEKPEIANAIYEHYLPIVAGGDLPQSDEGAI
                     VGLADKMDTIAGFFGVGMPPTGTADPYALRRQALGIINIILSREYPLSLNFLIDESLA
                     LLKDVLKKPAEGIKKDILEFFKGRLQNQLIAQGYAYDTVDAVLSANIDELVEVIEKVK
                     ALEEFRKHPDFEPVSIAFKRVDNILKDFKGGQFDVNLLVHDAEINLFSYFDNIKPRVE
                     KGIAEKDFAAALNKLAALRPHVDNFFDNVMVMDKDEKVRFNRQSLLAEISALFHKIAD
                     FSKIVTAG"
     gene            3331..5634
                     /locus_tag="PLR13_09595"
     CDS             3331..5634
                     /locus_tag="PLR13_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQC19634.1"
                     /translation="MVGSLDKISASAPGNSKIRKELLDITNRIHAAQNIKQILVDLKS
                     EILKLFNANSLTIYVVDKLRNEIYSMFLAGTQLKEIRLPINNKSIAGYVANTGAVINI
                     ENAYNAKELKSIEAELTFDDSWDKKTGFKTRQVLAAPIYHNNQLMGAIQIINKKVGEG
                     KFSEDEIGLALEIAEVLGVAFYNQQRFVRHRKTRFDYLLSHDLIKEDELESAWKESRE
                     KKISMENFLMQKHKISKADIGHAFEEFYNCRFIQYNDKLPIPGHLIKNLKKNFLRREL
                     WVPLEMVGDKIHVIIDDPNNIIKKDTIESLLKTKKIKYDVALEEDIIKYVNLFYNTPD
                     EEHSFTEILGRMEGEDGGPSSDDDEDLGEGVSETDSVIMQLVNKIVNDAYNRRASDIH
                     VEPNVGKKNVEIRFRVDGDCSLYQTVPFSYRAAIISRIKIMSNLDITIKRLPQDGKIK
                     FRRNTGDEIELRVATIPTQGGVEDVVMRILAKGETMPLQDMGLLPRNYNELINIISKP
                     YGMILVVGPTGSGKTTTLHAALHHINRPDRKIWTAEDPVEITQYGLRQVQVQPKIGFD
                     FAAAMRSFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLD
                     MGIDPLNFADALLGILAQRLVRTLCKSCKEPYHASKDEYDEIAKSYGAEEFAKLNIPY
                     NDDFKLYRAKGCPNCDGTGYKGRMGIHELLVGSEDIKRMIQKHETVEVMREKAISEGM
                     TTLLQDGLLKTLKGFTDFKQVRRVCIK"
     gene            5933..6208
                     /locus_tag="PLR13_09600"
     CDS             5933..6208
                     /locus_tag="PLR13_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19635.1"
                     /translation="MKKTLGTMIVAAAVILLTATFSFAEYAAAGAANFPFFQLGCLIV
                     GGLIMVSLKRKYDKMYVGEVVGIFALYTILMALFTNPVIETVKTIVS"
     gene            6251..6490
                     /locus_tag="PLR13_09605"
     CDS             6251..6490
                     /locus_tag="PLR13_09605"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQC19636.1"
                     /translation="MLATHKIHQKDFNNLKQVFMLSAWGFTMVVVSFLFLYIGHLVDG
                     LLGTTPNFMLGFFILGLFLCMMRLYQEACIRRKDV"
     gene            complement(6570..7184)
                     /locus_tag="PLR13_09610"
     CDS             complement(6570..7184)
                     /locus_tag="PLR13_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HQC19637.1"
                     /translation="MIRRHKGLACFIFVLLLTAGMVLASPYFLLYATDYKKADAVILL
                     LGPDFKARHKAARELVASGMADYLIISAYNKTYRNHDQDGTRQLVLDKHAPDADSENN
                     APFPGFYEDTHIEIIKAKKVMTAYGLKSAIFVSSPYHMRRIRFMAAKEFNFNDASFYF
                     VPTRYEKAPANFWELSSSDWRKVRREYSKIIWFFIYSAWLDYFL"
     gene            complement(7238..7786)
                     /gene="amrA"
                     /locus_tag="PLR13_09615"
     CDS             complement(7238..7786)
                     /gene="amrA"
                     /locus_tag="PLR13_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQC19638.1"
                     /translation="MELTDQEKNTLLDIAKNAIAAKINHRETPELNIDSNNLRLKRGA
                     FVTLKKQGRLRGCIGYIKAYKPLWETVQEMAVAAAFHDPRFPSLRAEEIQQLTFEISV
                     LSPLKRIADVNDIEVGRHGLYIIRGHHSGLLLPQVATEYKWDRETFLEETCHKAGLPP
                     DAWKDKETEIYIFSADYFGDIS"
     gene            complement(7863..8669)
                     /gene="amrB"
                     /locus_tag="PLR13_09620"
     CDS             complement(7863..8669)
                     /gene="amrB"
                     /locus_tag="PLR13_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQC19639.1"
                     /translation="MIAGSWYPGNPAVLRRDIEKYFDAVPEMTIDGNIVGLIAPHAGY
                     LYSGHVAAHAYKLVRGRTYDAVMVVGPSHRVAFRGISIFSKGGYETPLGVVPVEEALA
                     DKLKKKSPIVTDIPQAHLQEHAVEIQLPFLQVALGQFSFLPLVMGSQDAATCRELAEA
                     IADVSADKNLLIVGSSDLSHFYNYNAAKKMDAVALGHLKHGDSQGLLQSLENGDVEAC
                     GGGPMAVVMSVARMLKADRPCLLKYANSGDVTGDKESVVGYAAAVFYRSP"
     gene            complement(8695..11016)
                     /gene="cysS"
                     /locus_tag="PLR13_09625"
     CDS             complement(8695..11016)
                     /gene="cysS"
                     /locus_tag="PLR13_09625"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQC19640.1"
                     /translation="MSTFYKNVMEGIGNTPLVEITRLNPNKKVRILAKMESANPGGSI
                     KDRTALFMIESAESRGNLTHDKIILEATSGNTGIGLAMIAAVKGYKLCLIMPESASEE
                     RKKILRAMGAELYFTPAGHGTDGAIEVAYKMLRENPDKYFGTDQFNNEDNVAAHYFGT
                     GQEIWDQTDGKVTMVVASLGTSGTAMGISKRLKELNPQIKMVGVEPYLQHKIQGLKNM
                     RESYRPGIFNKNMLDEKINIQDEDAFEMSRRLAREEGLFVGMSSGAALFVALQQARQM
                     DEGLIVVIFPDGGERYLSTELFAVKKAISTFYLFNAFARKKTLFNPIKTDEVAMRTCG
                     PTIHDTPHLGNYRRLVVSDLINRYLIYKGYKVKHVLDIVDFGDKSIRGSEKESVALAE
                     YSKKYLQTFIEDTKFLNIRQDNICVKSSENVEAMLKMVEKLTDKEYAYEKLHSVYYNI
                     SKLADYGALSNIDLGKTRKVKSVDVDDYEKDSPADFALLKRSSLKELKRGIYYKTVWG
                     NVRPSWHLECAAISQKYLGDTYDIHVSGADETFPHCENVLAIQKAFSGHQGPNYWMNV
                     ELVMVEGRKMSRSLNNALSLSGLQKDGYSGSDIRLFLLSIHYRKPVNYSAGALQTAKN
                     TVRKIHTFIRRLHAIDDGVGGNGFAETDQLIYDLKQQFAAALDDDLNIAGALAALFSF
                     IGKINEPLTKGLINKNNAVKILKALENINIVLGVMDFEVGTIHRNIEELIDKRNAARK
                     ARDWKEADSCRARLAELGVDVLDTPRGVIWRFK"
     gene            complement(11105..11521)
                     /locus_tag="PLR13_09630"
     CDS             complement(11105..11521)
                     /locus_tag="PLR13_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HQC19641.1"
                     /translation="MIIYDLKCENGHAFEGWFKDRENFVLQHSKKLVSCPICNSSGVE
                     IVPSSITIMGKESRTENKSRSKELSGMGALKMLHQYIDKNFEDVGDKFAEVALKIHYG
                     DEEKRNIKGTSTPREEETLKEEGVQFFKIPVAKMDS"
BASE COUNT         3097 a         2966 c         2681 g         3027 t
ORIGIN      
        1 aatcgaatat aggagaaacg ttttttttag tcaattaatt agtcaaaaag tgttttatcc
       61 tttttatatt gacaccatac agatgaacat gttacttacg gccggtcatt ttttatttaa
      121 aaatcgacac gatgaacaaa ttttttgttt aacaccgcaa ggagaaaact gtgacttttc
      181 aagaattgat ttttgccctc gaaaaatact gggattccaa gggctgtgtc attcaacagc
      241 cttacgacat ggaagtggga gcgggaacgt ttcatccggc cacctgtctg cgcgctctgg
      301 gaccggaacc gtggaacgtg gcttacgttc agccctcgcg tcgtcccacc gacggccgct
      361 acggcgaaaa tcccaaccgt ctgcagcatt actaccagta tcaggtcatc atgaaaccgt
      421 cgccggtcaa tattcaggaa ctctacctgg attcgctgaa aagcttcggc attgatccgc
      481 tcgatcacga tatccgtttt gtggaagacg actgggaatc tccgacgctg ggcgcctggg
      541 gcctcggctg ggaagtctgg ctcgacggta tggaaatttc ccagttcacc tatttccagc
      601 aggtcggcgg cttcgattgc agtccggttt gcgccgaact gacctacggc acggaacgca
      661 tcgccatgta tattcagggc gtcaacaatg tttatgatct gcagtggacg gataaaatca
      721 aatacggcga cgtccatcac cgcggcgaag tggagttttc cacctataat tttgaagtag
      781 cggatattcc catgctgcgc aaattattcg atacgtatga agaggaagcc ctgcgcatcg
      841 ccgagaaaaa tctggccctg cccgcttacg attactgtct gaagtgttcg cataccttca
      901 acctgctcaa cgcccgcggc gccatcagcg tggcggaacg caccagctac atcggccggg
      961 tgagaaatct ggcgcgcatc agcgcggaac tgtacatgaa acaacgtgaa gaactgggct
     1021 atcccctttt atcccgctcg gcctatagtg acctttaggt tattcgcctc ggggataatt
     1081 cgaccatcaa atttcagtac acttcgtttc tgaaatttgg gtctgattaa agagataaaa
     1141 aatatctgga gacaaaatga gcaacgaact tctttttgaa attggcaccg aagaaattcc
     1201 cgcaggattt ttatccaaag cggtggtgga tatggaagag attatccgca aatcgtttac
     1261 ggataaacgc atcgccttcg acggcatcaa gtgcctggcg accccgcgcc gtctggttct
     1321 ttatatcgcc gatctgtctg caaagcagga agaccagact gttgaaaaac tgggaccggc
     1381 caaaaaagcc gcgttcgatg aaaacggtca gccgacgaaa gccgccatcg gttttgcccg
     1441 gggacagggc gtggacgttt cgcaactgga aaccatcaca accgaaaagg gcgaatatct
     1501 cggcgcgcgt aaaaccattg ccggacagcc caccaaggat ctgctgccgg acattctcaa
     1561 agatttcatg ctggcgattc ctttccgaaa atcgatgcgc tgggccgatt gcacgttgcg
     1621 tttcgcgcgc ccgatccact ggattctggc gctgtacagc ggcgaggtca ttcccctgaa
     1681 aatcgaagat attgaaagcg gcaacacctc ctgcggccat cggtttatga gcccggcccc
     1741 ctttgccgtc gccggctttg aagattatct gaacaaagcc agagaaaatt ttgtgatcgc
     1801 cgatccggcg gaaagaagaa aactgattct ggaagaagct gaaaaatcag cggcggaagt
     1861 cggcggaaaa cttttttaca cggatgatct gctggaaacg gtcagcttta tcgtggagta
     1921 tccggtcatc gtgcgcggcg gttacgaaga ggactatctg aaaattccca aagacgttct
     1981 gaccaccacg atgatttccc atcagaaata ttttccggtc gtcaatgaag cgggaaaact
     2041 gctgccctat ttcattgcgg tcagcaacac gaaaccccgc gatatttccg tggtggccaa
     2101 aggcaatgaa cgggttctgc gggcgcgact ggccgacgct tcgttctttt ttgaagaaga
     2161 caagaaagtt ccgctggaag atcgtgtgga atcgctcaaa aaagtcgtct tccatacgct
     2221 gctcggaacc tcgcataaaa aagttgcgcg tttccgcaaa ctggcgataa agattgcatc
     2281 caaggtcaaa ccgtccgcga agaaaaatgt cgaccgcgcg gcgcttttgg ccaaggccga
     2341 tctggaatcg ctgatggtcg gtgagttctc ggaactgcaa ggcatcatgg gccgtgaata
     2401 tgcgctcatc gccggtgaaa aaccggaaat cgccaacgcc atttatgaac attatttgcc
     2461 gatcgtcgcc ggcggagacc ttccgcaatc ggatgaaggc gccattgtag gccttgccga
     2521 taagatggac accatcgccg gcttcttcgg cgtgggcatg ccgccgaccg gtacggccga
     2581 tccctacgcc ctgcggcgtc aggcgctggg cattatcaat atcattttat cgcgcgagta
     2641 ccccttgagc ctcaactttc tcatcgatga aagcctggcg cttctgaaag atgtcttgaa
     2701 aaaaccggcg gagggaatca aaaaggatat tctggaattt ttcaagggac gtctccagaa
     2761 ccagttgatt gcgcagggtt atgcttacga taccgtggac gccgttttat ccgcaaatat
     2821 tgatgaactg gttgaggtca ttgaaaaagt caaggcattg gaggaatttc gcaaacatcc
     2881 cgatttcgag ccggtctcca tcgctttcaa gcgcgtggac aatattttaa aggattttaa
     2941 aggaggtcag tttgacgtaa accttcttgt tcacgatgcg gaaatcaatc tgttttctta
     3001 tttcgacaat atcaaacccc gcgtggaaaa aggcattgca gaaaaagact ttgcggcggc
     3061 gctcaataag ctggccgcgc tgcgtcccca cgtggataat ttcttcgaca acgtcatggt
     3121 catggacaag gacgaaaaag tgcgttttaa ccgtcagtcg ctgctggcgg aaatttcggc
     3181 tcttttccat aaaatcgccg acttctccaa aatcgtcacg gccggctgat cgtaaagcgg
     3241 caaccttcag gggacatgag gcgcattgac ataaattcaa atccttgcta ctatgatttt
     3301 catacagatt actaaggaat aatttgcctc atggtaggtt ctctagacaa aatttccgca
     3361 tcggcgcccg gtaattccaa aatacgcaag gaattgctgg atatcaccaa ccgtattcat
     3421 gccgcccaaa acatcaaaca gattctggtt gatttaaaga gcgaaattct aaaacttttc
     3481 aacgccaatt ccctgaccat ttatgttgtc gataaactgc gcaacgaaat ttattccatg
     3541 ttcctggccg ggacgcaact caaagaaatc aggctgccga tcaacaacaa gagcattgcc
     3601 ggttatgtgg ccaataccgg cgcggtcatc aatattgaaa atgcctataa cgccaaagag
     3661 ctcaaaagca tagaagctga actgacgttc gacgacagct gggacaagaa aaccggcttt
     3721 aaaaccaggc aggttcttgc ggcgcccatt tatcacaaca atcagctcat gggcgccatt
     3781 cagattatca acaaaaaagt gggcgaggga aaattttccg aggatgaaat cggccttgcg
     3841 ctggaaatcg ccgaggttct gggagttgcg ttttataatc agcagcgctt cgtccgtcac
     3901 agaaaaacgc gtttcgatta tctgttaagc catgatttga tcaaggaaga tgaactggaa
     3961 agcgcctgga aagaatcgcg tgagaaaaaa atctccatgg aaaattttct catgcagaaa
     4021 cataagatat ccaaagccga tatcggtcac gcttttgaag agttttataa ctgccgcttc
     4081 attcaataca atgataagct gcccatcccc ggacatttga tcaaaaattt gaaaaagaat
     4141 tttctacgcc gcgagctctg ggtgcctctg gaaatggtcg gagataagat tcacgtcatc
     4201 attgatgatc ccaacaacat catcaaaaaa gatacgatcg aaagtctcct gaaaacaaag
     4261 aagattaaat acgatgtcgc cctggaagaa gacattatca aatacgtcaa tctcttctat
     4321 aacaccccgg atgaagaaca ttccttcacc gaaatcctcg gccgtatgga gggagaagac
     4381 ggcggcccgt catcggatga tgatgaagac ctgggcgagg gcgtctctga aacagacagt
     4441 gtgatcatgc agctcgtcaa caaaatcgtc aacgacgcct ataaccgccg cgcatccgac
     4501 atccatgtcg aacccaatgt gggcaagaag aatgttgaaa tccggttccg ggtcgacggc
     4561 gactgcagtc tttatcaaac cgtgcctttc agctaccgcg cggccatcat ttccagaata
     4621 aaaatcatgt ccaatcttga tataacgatt aagagacttc cgcaggacgg caaaattaaa
     4681 ttccggcgca acacgggaga tgaaattgaa ttacgtgtcg cgacgattcc cacccagggc
     4741 ggtgtggaag atgtcgtcat gagaattctg gccaagggcg aaacgatgcc cctgcaggac
     4801 atgggccttt tgccccgcaa ttacaacgaa ctcattaaca ttatttccaa gccgtacggc
     4861 atgattctgg ttgtcgggcc gaccggttcc ggtaaaacca cgacgctgca cgcggccctg
     4921 catcatatta atcgcccgga cagaaaaatc tggacagcgg aagatcccgt ggaaatcacc
     4981 caatacggtt tgcgccaggt ccaggtgcag ccgaagatcg gtttcgattt tgcggcggcc
     5041 atgcgttcct ttctgcgcgc cgatccggat gtcatcatgg tcggtgaaat gcgcgatttt
     5101 gaaacagcca aaaccggcgt ggaagcctcc ctcaccggcc acctggtttt cagcaccctg
     5161 cacaccaaca gcgctccgga aaccatcgtc cgtctgctgg atatgggcat cgatccgctg
     5221 aatttcgccg acgcgttgct gggcatcctc gcgcaacgtc tcgtgcgcac gctttgcaag
     5281 tcctgtaagg aaccgtatca tgcttccaaa gacgaatacg acgaaatcgc taaaagttac
     5341 ggtgcggaag aattcgccaa actcaacatt ccgtacaatg acgactttaa attatacaga
     5401 gcaaaaggat gtcccaattg cgacggaacc ggatataaag gacgaatggg tatccatgaa
     5461 ctccttgtcg gttcggaaga tattaaaaga atgatacaaa aacatgaaac cgtggaggtc
     5521 atgcgggaaa aagccatcag cgaaggcatg accacacttt tgcaggacgg gctgctgaag
     5581 acgcttaaag gatttacaga cttcaagcag gtgcgccggg tttgtatcaa ataatccgct
     5641 tgtccgcgga ttttctccca tcaaaaattt ctcttcattc ggtgatctcc ttttttctta
     5701 tttgtccgtc aacggatccc cggcatcgac atttcgttaa acatctttaa tattttattc
     5761 ataaaaagac gaaataatcg tgttttccca taagcaataa ataacttatc taataataat
     5821 atttaataaa atcataactt tgcaaaattt aataagattt tttggcattc tggcacgttc
     5881 ttttcattag attaagacaa agaaaaaaat ttcctaaagg aggcaacaga tcatgaaaaa
     5941 aacattagga acaatgatag tggcggcagc ggttattctt ttaacggcaa ccttcagctt
     6001 tgcggaatat gcggcagccg gagcagccaa tttccccttc ttccagttgg gatgcctgat
     6061 cgtcggcgga ctgatcatgg tttctttgaa acgcaaatat gacaagatgt atgtcggtga
     6121 agtggtcggc atcttcgccc tgtacaccat tctgatggcg ctgttcacca acccggtcat
     6181 cgaaacggtc aaaaccatcg tcagctaagc attgctaaaa caacatctca cgggaaagga
     6241 ggaatgaatc atgctggcaa ctcataaaat acaccaaaaa gatttcaata atctcaaaca
     6301 ggtctttatg ctctccgcgt ggggtttcac catggtagtt gtttctttcc tgttcctgta
     6361 tatcggacat ctggtcgacg gtctgctggg aaccacaccg aattttatgc tggggttttt
     6421 tattctggga cttttcctgt gcatgatgag gctttatcag gaagcctgta ttcgaaggaa
     6481 ggatgtctga tcattaaaaa catgatctgc tatatcatat gaccctcctg caagacgcta
     6541 tgttttcagg agggtttttc tgatcaaaat caaaggaaat aatccaacca ggcggaataa
     6601 ataaaaaacc agattatttt actgtattcc ctcctgacct ttctccaatc cgaagacgat
     6661 aattcccaga aattggccgg cgctttctca tagcgcgtcg gaacaaaata aaaagaggcg
     6721 tcgttaaaat tgaattcctt cgccgccatg aatcgtatcc ttcgcatatg atagggagag
     6781 cttacaaaaa ttgccgattt aaggccatag gcggtcatga cctttttggc tttgatgatt
     6841 tcgatatgcg tgtcttcata aaaacccgga aaaggcgcat tgttttctga atcagcatcc
     6901 ggcgcatgct tatccagaac aagctgccgc gtcccgtcct gatcatgatt tctgtaggtt
     6961 ttattatagg ctgaaataat cagataatcc gccatgccgg aagcaaccag ttccctggcc
     7021 gccttgtggc gcgccttgaa atccggtccc agcaaaagaa tgacggcatc cgctttttta
     7081 taatccgtgg cgtaaagcag aaaatacgga gaagccagaa ccattccggc cgtcaacagc
     7141 agaacgaaaa tgaaacatgc caatccctta tgacgtctga tcataatcat ctcttcaatc
     7201 gttttacaaa atgagcatga attgtctgaa cggtttttca ggaaatgtcg ccgaaataat
     7261 ctgctgaaaa aatgtagatt tccgtttcct tatccttcca ggcatccggc ggcaatccgg
     7321 ctttgtggca ggtctcctcc agaaatgttt ctctgtccca tttatattcc gttgccacct
     7381 gcggcagcaa caaacccgag tgatgcccgc ggatgatgta caaaccgtgc ctgccgactt
     7441 caatgtcgtt gacatccgca atgcgcttga gcggactcaa gacggatatt tcaaaggtca
     7501 gttgttgaat ttcttccgct cttaaagacg ggaatcgcgg atcatggaaa gccgcggcaa
     7561 cggccatttc ctgcacggtt tcccagagcg gtttgtatgc tttaatataa ccgatgcaac
     7621 cgcgcaaccg cccctgcttc ttcagggtga cgaatgcgcc tcttttcaac cgcagattgt
     7681 tggagtcaat gttcaactcg ggagtttccc gatgattgat tttagcggca atcgcatttt
     7741 tggcgatgtc cagaagcgtg tttttttcct gatctgtcag ttccatgttg ctttcctttc
     7801 tccatcattc aatgacgcat acatttttcc gtcgttacga aagaaaataa acaagatccc
     7861 ttttaaggag aacggtaaaa aacggcggcg gcgtatccga caacggattc cttatccccc
     7921 gtgacgtctc cggaattggc gtatttcaaa agacacggcc tgtcggcctt taacatgcgg
     7981 gcaacagaca tcacaaccgc catgggtccg cccccgcagg cttccacgtc accattttcc
     8041 agactttgca aaagcccctg tgagtcgccg tgtttcaaat ggcccagggc aacggcatcc
     8101 attttctttg ctgcattgta gttataaaaa tgcgacaaat cggagcttcc cacaatcagc
     8161 agatttttat ctgccgatac gtcagctatc gcttcagcca gctcccggca ggtggcggca
     8221 tcctgactgc ccatgaccag cggtaaaaat gaaaattggc ccagggcaac ctgcagaaag
     8281 ggaagctgta tttccacggc atgctcctgc agatgggctt gcggaatgtc cgtgacgatc
     8341 ggactttttt tcttcagctt gtccgccaga gcttcttcaa cgggaacgac acccaaaggc
     8401 gtttcgtagc cgcccttgct gaaaatcgaa atgccgcgga aagcaacccg gtggctgggt
     8461 cctacgacca tcaccgcatc gtatgtcctg ccgcggacca gtttgtaagc atgggccgcg
     8521 acgtgtcccg aatacaagta gccggcatgc ggggcgatca acccgacgat attgccgtca
     8581 atcgtcattt cgggaacggc atcaaaatac ttttcgatgt ccctgcgcaa aacggcagga
     8641 tttcccggat accaggaacc ggctatcact gatttcttga taggattcat ctctctattt
     8701 aaaccgccag atgacacccc ggggggtgtc caaaacatcc acgcccagct ccgccagacg
     8761 ggccctgcag gaatccgctt ctttccagtc ccgggctttt ctggccgcgt tgcgtttgtc
     8821 gatcaattct tctatattcc ggtgaatcgt cccgacctcg aaatccatga cccccagaac
     8881 aatattgata ttttccagcg cttttaaaat ctttaccgca ttattcttat tgatcaagcc
     8941 tttcgtcaga ggctcgttaa tcttaccgat gaaagaaaac aaagcggcca gcgctcccgc
     9001 gatgttcaga tcgtcatcca acgcggcggc aaactgctgc ttgagatcgt atatcaactg
     9061 atcggtttct gcaaaaccgt tcccgccgac accgtcatcg atcgcatgaa gccgtcgaat
     9121 aaacgtatga atttttctga cggtattctt ggccgtctgt aaagcccccg ccgaataatt
     9181 gaccggtttc cggtaatgga tgctcagcag aaaaagacgg atatcgctgc cgctgtaacc
     9241 gtccttctgc aacccggaca gcgataaggc attgttcagc gaacgggaca ttttgcgccc
     9301 ctccaccatg accagttcga cgttcatcca gtaattgggg ccttgatgtc cggaaaaagc
     9361 cttctgaatg gctaaaacgt tttcgcagtg aggaaacgtc tcatccgcgc cggaaacatg
     9421 aatgtcatac gtgtctccca gatacttctg agaaattgcc gcgcactcca gatgccagct
     9481 cggtctgaca tttccccata ccgttttata ataaatccct cttttgagct ctttcagact
     9541 cgaccgcttc agcagggcaa aatccgccgg gctgtctttt tcgtaatcat caacatcaac
     9601 ggatttgacc tttctggttt tgccgagatc gatattggac agggcgccgt aatccgcaag
     9661 cttggatata ttgtaataca ccgaatgcag cttttcatac gcgtactctt tatccgttag
     9721 tttttcgacc atcttcaaca tcgcctccac attttcagag gacttgacac agatattgtc
     9781 ctgacgaatg ttgagaaatt tcgtatcctc aatgaacgtc tgcaaatatt ttttcgagta
     9841 ctccgcaagc gccacggatt ctttttccga tcctctgatg gatttgtctc cgaaatcgac
     9901 aatatccaga acatgcttca ccttgtatcc cttgtagatc aaataacggt tgatcagatc
     9961 ggataccaca aggcgccggt aattacccaa atgaggcgta tcgtgtatgg tcggcccgca
    10021 ggtccgcatg gccacttcat ccgttttaat cggattaaaa agcgtttttt tgcgcgcaaa
    10081 cgcattgaaa agataaaagg tggatattgc tttttttacg gcaaaaagtt ccgtgcttaa
    10141 atatctttct ccgccgtcgg ggaagatcac aacgatcagt ccctcgtcca tctgccgggc
    10201 ctgctgcaaa gccacgaaca gggctgcgcc cgaactcatg cccacaaaga gaccttcctc
    10261 ccgggccagc ctccgggaca tttcaaaggc atcctcatcc tgaatgttga tcttctcatc
    10321 cagcatgttt ttgttgaata tcccgggacg ataggattcc cgcatatttt tcaacccctg
    10381 tatcttatgc tggaggtagg gttcgacacc gaccattttg atctgcggat tcagttcttt
    10441 gagccttttg gaaatgccca tggccgtccc gctggtaccg agactcgcca ccaccatggt
    10501 cacttttccg tccgtctgat cccatatttc ctgtccggtg ccgaaataat gagcggcaac
    10561 attatcttca ttgttaaact gatccgtccc gaaatattta tccggattct cgcgcagcat
    10621 tttataggcc acttctatgg cgccgtcggt gccgtggccg gccggcgtaa agtataattc
    10681 ggcccccatc gcccgcagaa tcttttttct ttcttcactg gccgattccg gcatgatcag
    10741 gcacagttta tagcctttga cggctgcaat catggccagg ccgataccgg tgttaccgct
    10801 tgttgcttcc agaataattt tgtcgtgcgt cagattacct ctggattccg cgctttcgat
    10861 cataaacagg gcggttcggt ctttgatgga tccgccggga ttggcgcttt ccattttagc
    10921 cagaattctg acctttttat tgggattgag cctggtaatt tccaccagcg gcgtattgcc
    10981 gatcccttcc atgacatttt tatagaatgt actcatacgt aaaaatacct taaaataaaa
    11041 aaacccgcag gggttaaaaa ataatcagca tctttggaaa tctctattca ttcccggaca
    11101 tgaatcaact atccattttg gccacgggaa ttttaaaaaa ttgtaccccc tcttctttca
    11161 aggtttcctc ctcccggggc gtgctggtac ccttgatgtt tcttttttct tcatctccgt
    11221 aatgaatctt cagcgccact tccgcaaatt tatcgcccac atcttcgaaa tttttatcaa
    11281 tatactgatg cagcattttc agagcgccca tcccagacag ttccttgctc cgggatttat
    11341 tttccgtccg ggattctttt cccatgatcg taattgagga cggaacaatc tccacaccgg
    11401 agctgttaca gatcgggcag gaaaccagtt tttttgaatg ttgcagaaca aagttttccc
    11461 gatctttgaa ccacccttca aacgcgtgtc cgttttcaca cttcaaatcg tatataatca
    11521 taataaataa cctgttttat gacccatttg cattatttat aacatgcctg tttataaatt
    11581 acaattacat atcaattctt tcgagcttta ttcctttccg ttaaaatcat atttttgatg
    11641 taaatgataa agaaaacgga aaaatgaaca aaaaaagttg gacaattgca aataaatttt
    11701 atatcgtccg ccgtgagtcg ggatgtggcc cagtttggta gggcactgcg ttcgggacgc
    11761 aggagtcgcg c
//
LOCUS       DAONQZ010000070        11472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_6195_length_11472_cov_19.174652, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000070 DAONQZ010000000
VERSION     DAONQZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6195_length_11472_cov_19.174652"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..152
                     /locus_tag="PLR13_09635"
                     /pseudo
     CDS             1..152
                     /locus_tag="PLR13_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
     gene            complement(289..783)
                     /locus_tag="PLR13_09640"
     CDS             complement(289..783)
                     /locus_tag="PLR13_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19642.1"
                     /translation="MKPGASLTERFDGWFVKPIEKLKELPEGDGGFLALSAALFLCER
                     YYRALTDTLYGKRDDETFKVAAAKDLGLSPEDFNSFWIVYRNGVQHQGTPRHYIDKKN
                     QIKYFFHISDEFGGIPEIFKINAYKREIRLNVWKFADLIVSKFKTNPQVFEKAVSRTF
                     PAVK"
     gene            954..1814
                     /gene="prmC"
                     /locus_tag="PLR13_09645"
     CDS             954..1814
                     /gene="prmC"
                     /locus_tag="PLR13_09645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQC19643.1"
                     /translation="MTFRDILNEATRQLEAADISSARLDAEVLLAFCLQCDRLEFLKN
                     PDGMVGEKSLAAFQNCLSRRLRREPVAYITGRKAFWTFFLEVNQDVLIPRPDTEIIVE
                     ETLDICRKTETSILHILDVGTGSGAIALALAKELPQAKITATDFSAAALAVARKNALA
                     LKLADRIDFHPGNLFEPASGVFDIIVCNPPYISEDEYKELPAGVKNYEPAGALLAGKK
                     GTEFYEKLIRQAPDYLQKNGWLLLEIGAKQEEAVRRLLDKSGFYQDVAMRRDYAGLPR
                     VMRARRKTNG"
     gene            1807..3057
                     /gene="murA"
                     /locus_tag="PLR13_09650"
     CDS             1807..3057
                     /gene="murA"
                     /locus_tag="PLR13_09650"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQC19644.1"
                     /translation="MDKIMINGGKPLHGNVQISGAKNAALPVLASSLLTAGTNTFHNI
                     PDLMDIKTIKILLTSMGAEMEGGKTVRIRVDKINNPVASYDLVKTMRASILVLGPLVA
                     RAGVARVSLPGGCAIGARPVNLHLKALEDMGAHVELKNGYIEAKAKKLKGAEIYFDLP
                     TVTGTENIMMAATLAQGTTVLNNAAREPEVVNLADVLKGMGAKITGAGSDVITIEGVS
                     SLKPTEASIIPDRIEAGTFMIAAGMTRGEINVLGCNPHHLEALINKLRDTGMKISPIK
                     NGLTVSCGKKINSVDVTTLPYPGFPTDLQAQIMAYMAVGSGLSVITETVFENRFMHVS
                     ELLRMGADIVIQGANAVVRGVPKLRGAQTMATDLRASASLVLAALVAEGTTEISRVYH
                     IDRGYETIEKKFSALGADIKRVKS"
     gene            3054..4340
                     /gene="hisD"
                     /locus_tag="PLR13_09655"
     CDS             3054..4340
                     /gene="hisD"
                     /locus_tag="PLR13_09655"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQC19645.1"
                     /translation="MKMIKSSAAGFEKLFQKLRFREGAHSPELLAAVAKIVDDVAASG
                     DRALLKYMKKFDGYAPAAAELEATPAEKKKALAEVKPQEISIIKSAARRIERYHRCQV
                     AHGYKIRPETGVELEQRILPLHRVGIYAPGGKASYPSTILMAAIPARIAGAEEIVLVS
                     PAPAGKLNPYIAAAAEISGVHRIFKIGGAQAVAALAYGTETIPQVDKIVGPGNAYVAA
                     AKKMVFGQVDIDMIAGPSEVVVIADAKANASFAAADMLAQIEHDEMAAALLLTPSETF
                     AITVYREIRRQIKTSARKKIIEKSLDRYCAIFITGSLDEAVNIANRFAPEHLELMTQS
                     PDKILKKVRNAGSVFLGSYTPEALGDYLAGTNHILPTGGTARFSSPLGVYDFYKRMSV
                     LSFSRKAFEKLSRPTADFARMEGLDAHAHSVLIRGK"
     gene            4732..5511
                     /gene="ubiE"
                     /locus_tag="PLR13_09660"
     CDS             4732..5511
                     /gene="ubiE"
                     /locus_tag="PLR13_09660"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQC19646.1"
                     /translation="MISKNNKYPSVKKVTDEERIGIVRDIFSTITGSYDFLNHFLSLG
                     RDIAWRKFAAKKMVFPRSGKFLDIACGTGDLSIIACAAHQDMSVTGADFVHPMVRLAN
                     AKVRKKRLSGRIKFIQANALNLPFADNTFNVTAMAFGIRNIPQKDRAIKEMLRVTVPG
                     GQIMILEMTFIQNRLFKAAYYLYLNFMLPLLAKLFSKNAAAYHYLADSIMNFPKPEAF
                     AAFMEKTGVTDVQIFPRTFGITCLYLGEKPAQNNRATQNHH"
     assembly_gap    5644..5743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5744..>7761)
                     /gene="polA"
                     /locus_tag="PLR13_09665"
     CDS             complement(<5744..>7761)
                     /gene="polA"
                     /locus_tag="PLR13_09665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQC19647.1"
                     /translation="WRTVIISGDKDLLQLVNENVTMIDTMKDKTYDADAVKEKFGVGP
                     DKVVEMLGLMGDTSDNIPGVPGIGPKTAQRLIEEYGTVENVIKNAENLKNVKLRESFR
                     KHAEQARLSRQLANIRKDIPIDFDFQDAVVREPDREALSKLFTEFEFSSLLQEIRTDQ
                     IKPVLECKMVVNEKALAEFAACLKESHEFSMEFIYEKSQPAHLIGMAVSTGRHVFYVP
                     FGHTNVSGQLTVKDVLSAMAPAFNHTGIKKYFYDLKTALVCLDTLAVCSGDDLQLAAY
                     LLNPAKSSYELDDIAWEYLHEKIPSLNEIAGGKGKMYPLSLSPLDKMTAAAGQRAEAI
                     LSLTGVLAGQLEEIDVMDLYRKVEMPLLHVLAEMEKKGVLLDLALLKQMSAELTQLLA
                     LSESKIHHLAGEKFNINSPKQLQHILFEKLNLPAGKKTKEGYSTDVDVLTELARSHEL
                     PAEVLAYRSLAKLKSTYVDALPALVNPQTGRIHTSYNQTVTATGRLSSSNPNLQNIPI
                     RTLEGKRIRQAFIADPDCFLISADYSQIELRVLAHLSEDDALIDAFTSDEDIHSRTAS
                     DVFGVFPQMVNPDMRRQAKVINFGILYGMSAFGLAKELGVSMKTAQEYIDGYFNRYKK
                     VRLYLDGILEGARQNGFVSTLLNRRRYLPELKSKIPSVRQFAERMAINA"
     assembly_gap    7762..7861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7862..8209)
                     /locus_tag="PLR13_09670"
     CDS             complement(<7862..8209)
                     /locus_tag="PLR13_09670"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HQC19648.1"
                     /translation="MDAIDKKILNILQKEFPLEEQPFLIVGKRCGISEEETIRRVQNM
                     KNEGIIRRIGAVFDGAQLGRVSTLCAARVPEEKIDTFVKVVNASKNVTHNYLRNHEYN
                     IWFTVSAANAEELN"
     gene            complement(8232..9305)
                     /gene="ahbD"
                     /locus_tag="PLR13_09675"
     CDS             complement(8232..9305)
                     /gene="ahbD"
                     /locus_tag="PLR13_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HQC19649.1"
                     /translation="MKCNIMSIVLDNKVRMIAWEVTRSCNLNCVHCRAASSLGPYSGE
                     LSTEKCLQLVDQIAAVSTPVIILTGGEPLLRPDIFEIAAYGTNKGLRMVMATNGMLVT
                     PAVAQKMIQSGIQRVSISIDGKDAHSHDAFRQQKGAFNGAMQGIEALKNAKMEFQINT
                     TITTNNLDQIKDIFKLAKDIGAAAHHIFLLVPTGRGKDLAAQAITAADYEETLMWFHQ
                     ESLSCGIQLKATCAPHYFRIMHQNKPKEEKPVKKSGGHFHESTRGCLGGITFCFISHT
                     GQVQPCGYLELDCGNVKKKSFASIWENSEVFRNLRDYSKYEGKCGRCEFIKVCGGCRA
                     RAYEATGNYLAEEPLCLYQPKLK"
     gene            complement(9302..10276)
                     /gene="hemB"
                     /locus_tag="PLR13_09680"
     CDS             complement(9302..10276)
                     /gene="hemB"
                     /locus_tag="PLR13_09680"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQC19650.1"
                     /translation="MQFPEYRARRLRKNENVRRLIRETKLSVDDLVYPFFAVPGKNFK
                     KPISSMPGQFQLSVDHIVKEAKKVQELGIPAILLFGIPAKKDTMATGAFAKDGIIQQA
                     VKRIKNDVPDILVITDVCLCEYTSHGHCGMLEKDSVQNDETLEVLAETALSHARAGAD
                     MVAPSAMMDGQVAVIREALDENAFEDVPIMAYSAKYASSFYGPFREAAESTPQFGDRK
                     AYQMDPANADEAIREISLDVNEGADIIMVKPALAYLDIICRAKQEFDLPLAAYNVSGE
                     YAMIKAAAQLGWLDEERAMLESLTAIKRAGADIIITYFAPDVAKLLSK"
     gene            complement(10282..11469)
                     /gene="ahbC"
                     /locus_tag="PLR13_09685"
     CDS             complement(10282..11469)
                     /gene="ahbC"
                     /locus_tag="PLR13_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HQC19651.1"
                     /translation="MIGISKLYCGAVEPSDVLRYNRKSGDLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCIHCYSNSADIDYPDELTTEEGKKLIDDLAAFGSPVILFSGGEPLIRKD
                     LLELAQYATKKGMRAVISTNGTLITKDIAAKLQKVGLSYVGVSLDGLEKTHDRFRGKK
                     GAFAAAMDGIRNCREAGIKVGIRFTVNKHNLADVKDMFDLLRKEKIERLCFYHLVYTG
                     RGSKLRDEDLNHEQTRKLIDLIAAETKKMFDDGLSPEILTVDNHADGPYLYLKMKKED
                     PERAGEILELLEMNEGNSSGLGIGCVSWDGEVYPDQFWRNQSLGNVRNRPFSQIWTDE
                     KNELLMKLKEKKKHVHGRCAGCLWLNVCAGNFRARAESEGDIWGPDPACYLTDEEIHK
                     KED"
BASE COUNT         2734 a         2934 c         2760 g         2844 t
ORIGIN      
        1 gggcgagact tttggatgcc gaggtgcaga gacagaatgc cgaacaggcg cagacacgca
       61 aaagccaggt cggcagcggc gaccgctccg aacgcatccg cacctataat ttccctcagg
      121 gaaggattac cgatcaccgc atcaatctga cccgctacga tctggataat ttcatcaacg
      181 gcaatatcgg cgccatgatc gactcgctgg cggcgcatta tcaggccgaa gctctgaata
      241 aaacgaaaaa ctaaaaaagc atcaccttga tttttgagtg gttccgattt atttcactgc
      301 aggaaacgta cgggagaccg ctttttcaaa gacttgcgga ttcgttttaa acttgctgac
      361 gatcaggtcg gcaaacttcc agacgttcag ccggatttcc cgcttataag cgttgatttt
      421 aaaaatttcc ggaatcccgc cgaattcgtc ggagatgtga aagaaatatt tgatttgatt
      481 tttcttgtcg atgtaatgtc tgggcgttcc ctgatgctgc acgccgtttc tgtatacaat
      541 ccagaagctg ttgaaatctt ccggactcag ccccagatcc ttggcagcgg caactttaaa
      601 tgtttcatca tctcttttgc cgtaaagcgt gtctgttaaa gcgcggtagt atctttcgca
      661 gaggaacaac gcggcggaca gggccagaaa gcctccgtca ccctccggca gctctttcaa
      721 tttttctatc ggcttcacaa accagccgtc gaatctttcg gtcagacttg cacccggttt
      781 cataaacatc ctcgccaaac agcatgatgc ttctgtggct gtttgaggtc aattctttta
      841 aaatattaat ggcttggatt tataaacact tgacatttat tttgcaacac tttatttttt
      901 tcaaaaatca tggggttgat tccattatcg caccgtattc tgcaggagac atcatgactt
      961 tccgcgacat tctgaatgaa gcaacccggc aactggaagc ggcggacatt tcttccgcac
     1021 gtcttgatgc ggaggtcctg ctggctttct gcctgcagtg cgatcgcctg gaatttttaa
     1081 aaaacccgga tgggatggtc ggcgagaaat cgctggcagc gtttcaaaat tgtctatcca
     1141 gaagattgcg ccgggaaccg gtggcctaca ttacaggccg gaaagccttc tggacatttt
     1201 ttctggaagt caatcaggat gttctgattc cgcgtccgga cacggaaatc atcgttgaag
     1261 aaacactgga catctgccgc aaaacggaga cgtctatatt acatattctg gacgtcggca
     1321 caggcagcgg cgccatcgcc cttgcgctgg caaaagaact cccgcaggcc aaaattaccg
     1381 cgacggattt ttccgccgcc gcgttggccg tggcccggaa aaacgccctg gctctgaagc
     1441 tagcagatag aattgatttt catccgggaa atttatttga accggcaagc ggcgttttcg
     1501 atatcattgt ctgtaatcct ccgtatattt ccgaagacga atataaagaa cttcctgcgg
     1561 gcgtgaaaaa ttatgaaccg gccggtgccc ttctggccgg caaaaaagga actgaatttt
     1621 acgaaaaact gattcgtcag gcacccgatt accttcaaaa aaatggttgg cttttgttgg
     1681 aaattggcgc taaacaggag gaagcggtgc gccggctgct agacaaatcc ggattttatc
     1741 aagacgtcgc catgcgtcgg gattatgccg gattgccgcg cgttatgaga gcaaggagaa
     1801 agaccaatgg ataaaatcat gattaacggg ggcaaaccgc tccatggtaa tgttcagatc
     1861 agcggcgcga aaaacgccgc cctgccggtt ctggcttcgt cattactgac ggcgggaacc
     1921 aacacatttc ataatattcc tgatttgatg gatattaaaa cgataaaaat tcttttgacc
     1981 agcatgggcg ccgaaatgga aggcggaaaa accgtccgca tccgcgttga taaaataaac
     2041 aatcccgtcg cgtcctatga tcttgtgaaa accatgcggg catcgattct ggttttggga
     2101 ccgctggtcg cccgcgccgg ggtggcgcga gtttcgcttc cgggaggctg cgctatcggc
     2161 gcgcgtccgg tcaatctgca tctcaaggcg ctggaagaca tgggcgcgca cgtcgaactc
     2221 aaaaacggct atatcgaggc caaagccaaa aaattgaaag gggcagaaat ttatttcgat
     2281 ttgcccacgg taaccggcac cgaaaatatc atgatggcgg caaccctcgc ccagggaacc
     2341 accgtgttga acaatgccgc gcgcgaaccg gaagtcgtca atctcgctga cgtcctcaaa
     2401 ggcatgggcg cgaaaatcac cggagcgggg tcggatgtca tcaccatcga aggcgtttct
     2461 tccctcaagc cgacggaagc gtcgatcatc cccgaccgga tcgaagccgg aacgtttatg
     2521 attgcagcgg gtatgacacg cggagaaatc aatgttctgg gatgcaatcc tcatcacctg
     2581 gaagcgttga tcaacaaact gcgcgatacc ggcatgaaaa tttcgccgat taaaaacggc
     2641 ctgacagtat cgtgcggcaa aaaaatcaac agcgtcgatg taacaacgct gccttatccc
     2701 ggcttcccca cggatttgca ggcccagatc atggcttaca tggcggtcgg ttccggcctg
     2761 agcgtgatca cagaaaccgt tttcgaaaac cggtttatgc atgtcagcga acttttgcgc
     2821 atgggcgccg atattgtaat tcagggagcc aacgccgtcg tccggggcgt tcccaaactg
     2881 aggggtgcgc aaaccatggc cacggattta cgggcatcgg cctcgctggt tctggcggcg
     2941 ctggtggccg aaggcacaac ggaaatttcc cgcgtctatc atatcgaccg cggttatgaa
     3001 accatcgaaa agaaattttc cgccctggga gcggatatca aaagggtaaa atcatgaaga
     3061 tgataaaatc cagcgctgcc ggatttgaaa aactttttca aaaattgcgc ttcagagaag
     3121 gggctcactc acctgaactc ctggcagctg tggccaaaat tgtggacgat gtcgccgcct
     3181 cgggcgatcg ggcgcttctg aaatacatga aaaaattcga cggctacgca ccggcagcag
     3241 ctgagttgga agccacacct gcagaaaaga agaaagcgct ggctgaagta aaaccacagg
     3301 aaatcagcat tataaaatcg gcggcacggc gcatagaacg ttatcaccgc tgtcaggttg
     3361 cccacggata caaaatccgt ccggaaaccg gcgttgaact tgaacagcgc atattgcctc
     3421 tgcaccgggt gggaatttac gctcccggcg gcaaggcgtc ctatccatcc accattctga
     3481 tggcggccat tccggcgcgc attgcgggcg ccgaagaaat tgttctggtc agccccgcac
     3541 cggctggaaa gctcaatcct tacattgccg ccgccgctga gatcagcggc gttcatcgca
     3601 ttttcaaaat cggcggcgcg caggcggtcg cggcgctggc gtacggtacc gaaacaattc
     3661 ctcaggtgga caaaatcgtc ggtcccggaa acgcctatgt cgccgcggcg aaaaagatgg
     3721 tcttcggtca ggtggatatt gatatgattg cgggacccag cgaagtggtg gttattgccg
     3781 acgccaaggc caatgcgtct tttgcggcgg cggatatgct ggcacaaatc gagcacgatg
     3841 aaatggcggc ggcattgctt ttgacgccat ccgaaacatt cgccataacg gtgtaccggg
     3901 aaatcaggcg gcagattaaa acctcggcac gaaaaaagat tatcgaaaaa tcgctggaca
     3961 ggtactgcgc cattttcatc accggaagcc ttgatgaagc cgtgaatatt gcgaatcgct
     4021 ttgcgcccga acatctggag ttgatgaccc aatcgcctga taaaatttta aaaaaggtca
     4081 gaaatgccgg atctgttttt ctgggctcct atacgccgga agccctgggc gattatctgg
     4141 cgggaaccaa tcacattctg cccacgggag ggacagcccg tttttcatcg ccgctgggcg
     4201 tatatgattt ttacaaacgg atgagcgttc tttctttctc cagaaaagcg tttgaaaaac
     4261 tgagcaggcc gacggccgac tttgcccgga tggagggcct cgacgctcat gcccattctg
     4321 ttctgattcg cggcaaataa cagttataca aaagatttga ttgatatcat gatttattcc
     4381 gatgctctgg tggacattac cgataaaaac attcttttta aaaattacag tttcttcgac
     4441 cgcaacagat cggttttttt cacgacatcg aaaaattgtc atcaaaaaac cttccgtccg
     4501 gacgggcaaa ttccgctttc acggaacagg ggatttccac gcctggtttg cgaaagattt
     4561 tgaaagacac aaacacgacg ggatgttcat cgcttttatc cgccgccaat ggtggcggat
     4621 cggatttaca gttgaacgtt cagatgccgt tatgaaaatc tttcaggaaa gagggttgat
     4681 cagaccggcg ggtaagcatc ctttgttgac gaatcgaaag aaagtttgca gatgatttca
     4741 aaaaacaaca agtatccttc cgtaaaaaaa gtaaccgatg aggaacgcat cggcatcgtc
     4801 agagacatat tttcgaccat taccggcagt tatgattttt taaatcattt tttgagcctg
     4861 ggtcgcgaca tcgcctggcg gaaatttgcg gcaaagaaaa tggttttccc ccgttccggg
     4921 aagtttctag acatcgcctg cggaacggga gacctgtcta tcattgcctg cgccgcacat
     4981 caggatatgt cggttaccgg agccgatttt gtccatccga tggtccgcct ggcaaacgcc
     5041 aaggtccgga aaaaaagact cagcggcagg atcaaattca ttcaggccaa cgcactgaat
     5101 cttccgttcg ccgacaatac ctttaacgtt acggcaatgg ctttcggaat caggaatatt
     5161 cctcaaaaag accgggcgat taaagagatg ctgcgcgtca cggtgccggg cgggcaaatc
     5221 atgattctgg aaatgacctt tattcaaaac cgccttttca aggccgccta ttatctctat
     5281 ctgaatttta tgcttccgct gcttgccaaa ctcttctcta aaaacgcggc ggcctatcat
     5341 tatcttgccg attccatcat gaactttccc aaacccgaag cgtttgcggc attcatggaa
     5401 aaaaccggcg ttaccgacgt tcaaattttt ccgcggacgt tcggcattac ctgtttatat
     5461 ctcggggaaa aacctgctca aaacaaccgc gctactcaaa atcatcattg aaaatctctg
     5521 atcagtgcgc ttcgtcccag tttttgccgg aggcaatttc caccttcagc ggtactttca
     5581 gcctgatgac ctcttccatt tcttttttga ccagcgccat gacgtccgct ttctcacctg
     5641 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcattga tcgccatgcg
     5761 ttcggcgaac tggcgcacgg aaggaatctt gctttttaat tccggcagat agcggcgccg
     5821 gttgagcagc gtggaaacaa agccgttctg gcgcgccccc tccagaatac cgtccagata
     5881 aaggcgcact tttttatagc ggttaaaata tccgtcaatg tattcctggg cggttttcat
     5941 ggacacgccc agttcttttg ccagaccgaa agcgctcatt ccataaagaa tgccgaagtt
     6001 gatcactttg gcctgccggc gcatgtccgg gttcaccatc tggggaaaaa caccaaagac
     6061 atcggaggcc gtccggctgt gaatatcttc atcggaggta aaggcatcga tgagcgcgtc
     6121 atcttcagac agatgcgcga gcacccgtaa ttcgatctgc gaataatcgg cggagatcag
     6181 aaagcaatcc ggatccgcaa taaaggcctg gcggattctt tttccctcaa gcgttctgat
     6241 cgggatattc tgcaggttgg gattggagct cgacaaccgg cccgttgccg tcaccgtctg
     6301 gttataggat gtgtggattc tgcctgtttg cggattgacc agggccggta aagcatccac
     6361 atacgtggat ttgagcttgg ccagcgagcg gtaagccagg acttccgccg gcagctcgtg
     6421 gctgcgggcc aactcggtca gtacgtccac atcggtggaa taaccttcct ttgttttttt
     6481 cccggcaggc aaattcaatt tttcaaacaa aatatgctgc agctgcttgg gggaattaat
     6541 gttgaatttt tcgccggcca gatgatgaat tttcgattcc gacaacgcca gcagttgcgt
     6601 aagctccgcg gacatctgct ttaacagcgc cagatcgaga agcacgcctt ttttctccat
     6661 ctccgccaga acgtgcagca gcggcatttc aaccttgcgg taaagatcca tgacgtctat
     6721 ttcttccaac tgaccggcaa gtacaccggt aagactcagg atggcttcgg cgcgctgtcc
     6781 cgcagcggcg gtcattttat cgagaggcga cagcgacagc ggatacattt tgcccttgcc
     6841 tccggcgatt tcattcaggg acggaatttt ttcatgcaga tattcccagg cgatgtcatc
     6901 cagttcataa gacgattttg cgggattgag aagatatgcc gccagctgca aatcgtcgcc
     6961 ggagcaaact gcaagagtgt ccagacaaac aagcgcggtt tttaagtcat aaaaatattt
     7021 ctttattccg gtatggttga aggcgggagc cattgcgctc aaaacgtctt tgaccgtcag
     7081 ttgccctgag acattggtgt gcccgaaggg aacgtaaaaa acatgcctac cggtactgac
     7141 agccataccg atcagatgtg ccggctgact tttctcatag atgaattcca tcgaaaattc
     7201 gtggctttct ttcagacatg cagcaaattc agccagcgct ttctcattga caaccatttt
     7261 gcattccaga accggtttga tctgatccgt tctgatttcc tgcaaaagcg aggagaattc
     7321 aaattcagtg aacagttttg acagagcctc cctgtccggc tctctgacga cggcatcctg
     7381 aaaatcgaaa tcgatcggga tatcttttct gatattggca agctgccggc tgagcctggc
     7441 ctgctcggca tgcttgcgaa aactttcgcg cagtttgaca ttttttaaat tttcagcatt
     7501 cttgatcacg ttttcgaccg tgccgtattc ttcgatcagc ctctgcgccg ttttgggacc
     7561 gatgccgggc acgcccggaa tgttgtcgga ggtatcgccc atcaggccca gcatttcaac
     7621 caccttgtcc ggaccgacac cgaatttttc tttcaccgca tctgcgtcat atgttttatc
     7681 tttcatggta tcgatcatcg tgacattctc attaaccagc tgcaacaaat ccttgtcgcc
     7741 ggaaatgatg accgtgcgcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nattgagttc ttcggcgttc gcggcgctga cggtaaacca gatattgtat tcatgattgc
     7921 gcagataatt atgcgtcaca ttcttactgg cattgacaac cttaacgaag gtgtcgattt
     7981 tttcttccgg aacacgggca gcgcaaagcg tgctgacacg acccagttgt gctccgtcga
     8041 agacggcgcc gatacggcgg atgattcctt cgttcttcat attctggacg cgtctgatag
     8101 tctcttcttc gctgatgccg cagcgttttc ccacaatgag aaaaggctgc tcctccagcg
     8161 gaaattcctt ctgtaaaatg tttaatattt tcttatctat tgcatccata atctatttta
     8221 atttcggtaa tctattttaa tttcggttgg tataagcaca acggctcttc cgctaaatag
     8281 ttgcctgtag cttcataagc acgcgcgcgg caaccgccgc acacctttat aaactcgcag
     8341 cggccgcact tgccttcata tttactgtaa tcccgcaggt tacgaaacac ttccgaattt
     8401 tcccatatgc tcgcgaagct tttcttcttc acattgccgc aatccagttc cagatagccg
     8461 cagggttgca cctggccggt gtgcgaaata aagcaaaatg tgattccccc cagacagcct
     8521 ctggtcgatt catgaaaatg accgcccgat ttcttcaccg gcttttcttc tttgggtttg
     8581 ttctgatgca taatgcggaa ataatgcggc gcgcaggtgg ctttgagctg gattccacaa
     8641 ctcaggcttt cctgatgaaa ccacatcagc gtctcttcgt aatcagcggc agtaatagcc
     8701 tgcgcggcaa gatctttccc ccggcctgtc ggcaccagca agaatatatg atgagcggcc
     8761 gcaccgatat cttttgctaa tttaaaaata tccttaatct gatcaaggtt attggttgtg
     8821 atggtcgtgt tgatttgaaa ttccatttta gcatttttga gtgcttcgat gccttgcatc
     8881 gcgccgttaa acgcaccttt ctgctgacga aatgcatcgt ggctgtgcgc gtctttcccg
     8941 tcaatgctga tgctgactct ttgaatacca ctctgtatca ttttttgcgc aactgccggt
     9001 gtaaccagca tgccgttggt cgccatcacc atgcgcagac ccttgttggt tccgtaggcg
     9061 gcaatttcaa aaatgtccgg acgcaggagc ggctcaccgc cggtcaggat aatcactggc
     9121 gttgatacgg ctgcaatttg atcgaccaac tgcaagcact tttcagtgga gagctccccc
     9181 gaataagggc cgaggctcga agccgcgcgg caatggacac agttgagatt gcagctgcgc
     9241 gtcacttccc acgcaatcat acggacttta ttatcaagga caatcgacat aatattacat
     9301 ttcatttgct cagcagtttc gccacatccg gagcgaagta ggtgatgata atatcggcgc
     9361 cggcgcgttt gatggctgtc agcgattcca gcatcgctct ttcttcatcc agccagccga
     9421 gttgcgcggc cgctttgatc atcgcatatt caccgctgac gttatacgcc gccaaaggta
     9481 aatcaaattc ctgcttcgca cggcagataa tgtccagata agccagagcg ggtttcacca
     9541 tgatgatgtc cgcgccctca ttcacatcca gtgaaatttc tctgatcgct tcgtctgcat
     9601 tggcaggatc catctgatac gctttccggt cgccgaactg cggcgtactc tcggcagcct
     9661 cccggaaagg cccgtaaaac gacgaagcgt attttgccga ataagccata atgggcacat
     9721 cctcgaacgc gttttcatcc agcgcttcgc ggatcaccgc cacctgtccg tccatcattg
     9781 ccgagggcgc caccatatcg gccccggcac gtgcgtgcga aagagcggtt tccgcgagca
     9841 cctccagcgt ttcgtcattt tgaacgctat ctttttccag cataccgcag tggccgtggc
     9901 tggtatattc gcacaggcag acatccgtaa tgaccagaat gtcgggcacg tcatttttaa
     9961 tacgcttcac cgcttgctgg atgatgccgt ccttggcaaa cgcgccggtg gccatcgtat
    10021 ctttcttggc gggaatgccg aagagcagaa tcgctggaat tccgagctcc tgcacctttt
    10081 ttgcttcctt gacgatatga tcaacggaca gctgaaactg gccgggcatg gacgaaatcg
    10141 gttttttgaa attttttccg ggcacggcaa aaaacggata gaccagatcg tcaacgctga
    10201 gttttgtttc acggatcaga cgccggacgt tttcattttt tctcaatctg cgcgcacgat
    10261 attccggaaa ttgcatgttc tttagtcctc ctttttatga atctcttcat cggtcagata
    10321 gcaggcggga tcaggtcccc agatatctcc ctccgactct gcccgggcac ggaaattgcc
    10381 ggcgcagaca ttgagccaca aacatcccgc gcagcggcca tgcacgtgct tctttttctc
    10441 cttgagtttc attaacagtt cattcttttc atccgtccag atttggctga aaggccgatt
    10501 gcggacatta cccaaagact gattgcgcca gaactgatcg ggataaacct cgccatccca
    10561 gctcacgcaa ccgataccca gtcccgaaga attgccttcg ttcatctcaa ggagttccag
    10621 aatttctccg gcgcgttcgg gatcttcttt tttcatcttc agatacagat acgggccgtc
    10681 ggcgtgatta tccaccgtca gaatttcggg agacaacccg tcgtcaaaca tttttttggt
    10741 ttcggcggca atcaaatcga tcagctttcg ggtttgctcg tggtttaagt cttcatcacg
    10801 cagtttggaa ccgcgaccgg tgtataccag atgatagaaa cacagccgtt caatcttttc
    10861 ttttcttaaa aggtcgaaca tatcctttac gtcggccaga ttatgtttgt tgaccgtaaa
    10921 acggataccg accttgattc ccgcctcgcg gcaattgcgg atgccgtcca tggctgccgc
    10981 aaacgcacct ttcttcccgc ggaaccggtc atgcgttttt tccagaccgt caaggctcac
    11041 gccgacatag gaaaggccga ctttctgaag tttggccgca atgtctttgg taatcagcgt
    11101 accgttggta gaaataaccg cacgcattcc cttcttcgtc gcgtattgcg ccagttccag
    11161 caaatccttg cggatcagcg gctcgccgcc ggaaaaaaga atgaccggag agccgaaagc
    11221 cgccagatcg tcaatcagtt tcttgccttc ttccgtggtc agttcgtcgg gataatcgat
    11281 gtcggcggaa ttggaatagc agtggatgca ttttaaattg cagcggcggg tcatgttcca
    11341 gacaactaca ggctttttgt ctgccgaaaa ctgcaacagg tgcgatggga gatcacccga
    11401 tttgcgatta tagcgcaaaa catcggacgg ttccacagcc ccgcaatata atttggagat
    11461 tcctatcatt ta
//
LOCUS       DAONQZ010000071        11404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_6250_length_11404_cov_17.451317, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000071 DAONQZ010000000
VERSION     DAONQZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_11404_cov_17.451317"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLR13_09690"
     CDS             <1..434
                     /locus_tag="PLR13_09690"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQC19652.1"
                     /translation="QLRKNIDAVPHAVGVNNHMGSKFMMDDEKLSVIFKELQRKKLFF
                     IDSRTSPDTRAYAIADDMGFRIAERKIFIDHHRDYHAIYSQLMHVAQSNDPAPKIIIG
                     HPYPETVRALQAATKVMREKGVSVVPASRIIKKTNKSPEKS"
     gene            484..2172
                     /locus_tag="PLR13_09695"
     CDS             484..2172
                     /locus_tag="PLR13_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19653.1"
                     /translation="MKRIFICFVIIMLVSLSGCVTLPNVQHAPSGDKFQQQASYHYLL
                     SVFLRNDGNLDEAIRELEQAYAADPDSAYLVIELMSLYAQKGDVKSAVAHGEEALARD
                     PDNVDVHLIMGGLYLNMREYSKAVREHKKVIELDPKNLVAHLYLGIIYAEEKRFDRAE
                     ETFKKMLKIDPDNIMGVYYYGKVLEKMKRLDEAEKMFKKAMSLNPYFEMALFDLAALY
                     EKQEKLDKAIEIYRNYLAIDPTRLNLRVRTGELLIKKKKYEEAEKEFQEVLKIDPDNR
                     DIKVALGLLYFDMKKFDLAAAEFSSILQKNVLDDKVRYLLATTYDQQKNNVRAVEEFT
                     GIASSSELYVSAQLRAAMILKEEGKVTEAVAVIKKSIKKKNDQVPFYLLLSSFYDETG
                     DNASSEAILQDGLKVLPRSTDLHYALGVLYDKTNRFEESIMSMKKILDIDPKNAEALN
                     FIGYSYAVRDMHLDEAEAMILRALKIKPGNGYLLDSLGWVYFKKNDLKNAEKYLKEAW
                     TLLPDEIEIIEHLGDLYVRQNRLKDAADMYERGMKIDPGNDVLKKKREDIRGTR"
     gene            2248..2985
                     /locus_tag="PLR13_09700"
     CDS             2248..2985
                     /locus_tag="PLR13_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQC19654.1"
                     /translation="MLCLCSCALQQASGVARDVYPEKRILSTSGGLAEEDVFQALAKI
                     DFKTVDASYHVRAALVLRRPGYVRLEVLPVIGNPDLLLTATPDTMTVFVPSKKELYRG
                     RPTRSNLEKFLPWSMDIEDMVLVLTGTYPFCTTERLAEDVSSEGRLWRMTCKKSSSES
                     QIIWVQEGSQKLMRLLCTDETGSEKYNVQYFYGGQSALPEKLVIRLANGSTALTVAYS
                     DVRVEKTKDLSVFNLTVPSDVEQNILE"
     gene            complement(3010..3822)
                     /gene="ybgF"
                     /locus_tag="PLR13_09705"
     CDS             complement(3010..3822)
                     /gene="ybgF"
                     /locus_tag="PLR13_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQC19655.1"
                     /translation="MEKFRYFILFLVVLFLAGCASSGDLRALRSEMSQRTDEKISSLE
                     AEVNALKENATALESMRKGQANTMADLAELRENLQQLRGQVETLKKDASQSSRKDEQL
                     LNNMLLRINFIENFLEIGSKNTSDASDKTGKVNGGSVAKDTGSKQDKDKAYSAAYQIF
                     QEGNYAKARNEFQTFLTTYPNSEYSDNAQFWIGECYFFEKKYESAILEYEKVTKNYPK
                     GNKVPYALLKQGLSFLKLGDKTSAKLLLQEVIKNYPNTSQARIARSNLQDIK"
     gene            complement(3844..4413)
                     /gene="pal"
                     /locus_tag="PLR13_09710"
     CDS             complement(3844..4413)
                     /gene="pal"
                     /locus_tag="PLR13_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQC19656.1"
                     /translation="MNMQIHLRLVLVVFIAGLMMIAGCAEKKSVITSDAAQEQQTTPA
                     QTTPSQEDMSSKDITGSQIASEEKSVDLEKMARAQSPVNDIHFDFDSSSIRPDARETL
                     RENADYFMNNRISSIIIEGHCDEKGTNEYNMALGERRAQETKKYLVNLGVKESLMQTV
                     SFGEENPLDPASNEDAWAKNRRAHFVVNP"
     gene            complement(4613..5935)
                     /gene="tolB"
                     /locus_tag="PLR13_09715"
     CDS             complement(4613..5935)
                     /gene="tolB"
                     /locus_tag="PLR13_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQC19657.1"
                     /translation="MTKRVLILTLIFFFCAWPICSNRADSKVYVDIDSPAFRLIPIAI
                     CDFHPASGPVGHVASETTVSERVKKDLTLTGLFNILNKQSFLENPDAVSTTAVGKIRF
                     ADWLAVGADYLVKGRIFQKDREITVEAYLYDVARGELIFGKKYQAPAEKTKAVARAIA
                     SDIMLALINDAGDFNTEIAFVSKTGLRSDIYAVSYDGADVRKVTHHQSILMAPRWSPD
                     GNGMAFTSFKRGRPEIYFLNLKNRSEKRLASFGGLNLCGSFSPDGRKLLMTLSKDGNE
                     EIYALDVQTLQLQRLTRHSAIDVSPSWSPDGKQIVFVSNRGGSPQIYTMDADGNNVKR
                     ITYKGSYNSSPSWSPRGDRIIYEGLTADRYQIFSVDTEGNNPAQLTSDNANNESPFWS
                     PSGRQIVYVSRKHSSSQILIMNANGTNPRLLYEQSNRLAMPAWSGRLR"
     gene            complement(5986..>6313)
                     /locus_tag="PLR13_09720"
     CDS             complement(5986..>6313)
                     /locus_tag="PLR13_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQC19658.1"
                     /translation="SGRTAMTDAEVNAQANEYIETVWSRVKQNWSIPQSLLPDNNITT
                     IVDVRIARNGALLYAGFEKRSGNRYFDDSALRAVKKSGPFPPLPHWIRDNHIEIGIRF
                     HSSELR"
     assembly_gap    6314..6413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6475..6900)
                     /gene="tolR"
                     /locus_tag="PLR13_09725"
     CDS             complement(6475..6900)
                     /gene="tolR"
                     /locus_tag="PLR13_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQC19659.1"
                     /translation="MVRGSRRKNNSSKPLAEINVTPLVDVMLVLLIIFMVTAPMLSMG
                     IDVNLPRVKAKSVDVAEEKLVLTVNETREIFLNKTKMTLGELKPKLEAIFSNRIDREI
                     FLRADKNVPYGFVVEVMSEVRKAGVDKLGMITEPPEETK"
     gene            complement(6919..7644)
                     /gene="tolQ"
                     /locus_tag="PLR13_09730"
     CDS             complement(6919..7644)
                     /gene="tolQ"
                     /locus_tag="PLR13_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HQC19660.1"
                     /translation="MKDFGALDLSGSVNGSLLSMILDAGLMVKFVLLVLFIFSVVSWA
                     IIFIKYRYYRKIQKENEAFNDDYLKSSKLSDVLPAAKKYSYSTTAEVFRVGYKELTQT
                     NRPAAGESAQGRDDISLSSLDNLERSMNKASATEMTKLESSLGFLATTGSASPFIGLF
                     GTVWGIMDTFKGIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNQSKN
                     MVQEMENFSAEFLNIVERYLVRK"
     gene            7816..9324
                     /locus_tag="PLR13_09735"
     CDS             7816..9324
                     /locus_tag="PLR13_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19661.1"
                     /translation="MKRKRILTQFLLYFLMLMFLIVAGCSSGGSDDDDDDQNQQQLFV
                     TVSGKVTEADGITGVQGVNISAMYSLTILNVKVWEGSISSDLFSYIITDANGNYTLRL
                     PAALVEQYNVILTPGKKGYTFTPARRTFDVGLTDITGVNFTAAEASVYGQADLAGTWR
                     TNMLRTGTENGWVRARLTIGATGVATCESYETSTSAGDTTCPADFDLTFTVNPDTGAI
                     IQSGAYAAGDGGHMTMNSDMDFAAGTATAGTNYQIMIAQKEGTTNYSNANLASHNFVV
                     HGMVVGQTNEWWYGTGATDTALVTTMSEEVGPTGAPSATINGVTLAVAVDGVVTLVDA
                     LGARASDFEGFLSVDKKTMVGTYTDINGDYQLMIVQITDGQAVANLTGSSYNHLLGVG
                     DDVFWAHHDINVSRFETSFLPDILALTFNLDVMLSYNWNLSLAYTPIQKLGLVSVKKL
                     TIAPNGQAIVTNPETDAIVFHGQLAFDGTFMVGTETMELTEGTFYTLNVITH"
     gene            complement(9408..>10047)
                     /locus_tag="PLR13_09740"
     CDS             complement(9408..>10047)
                     /locus_tag="PLR13_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19662.1"
                     /translation="LHLPEFTMLEWYVAGFDYRQLMAQCRNLIVAVAQNQGLTEIKRD
                     NRTIALTPPWESITVEDAFLRYAPLTLDEALGQGTFDEILVDCIEPRLGIGKPTFLYD
                     YPASLAALAKLKDNHRTVSERFELYIGGLEIANGFSELTDAVEQRKRFEEALKKRAEK
                     KWSEYLMPEKFLQSLGHLPPCAGIALGIDRLVMIITGANCIDDVVAFIPENL"
     assembly_gap    10048..10147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10148..10524)
                     /locus_tag="PLR13_09745"
     CDS             complement(<10148..10524)
                     /locus_tag="PLR13_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQC19663.1"
                     /translation="MYTASDLRKGLRLKIDGEPYVIVEFNFVKPGKGQALYRTKLKNM
                     INGNQFERTFRSVDTFEQADLREKTMQYLYKEGDKFCFMDNETFEQVYLTEEQVDDAV
                     NFMTDNIEVEILLFEDRPIGISLP"
     gene            complement(10580..>11404)
                     /locus_tag="PLR13_09750"
     CDS             complement(10580..>11404)
                     /locus_tag="PLR13_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HQC19664.1"
                     /translation="RAAEDPLQEDLHMPVPKLVHRYPDRCLAIVTETCAVYCRHCNRK
                     RFWSRPASLNLTDRLQKITRYIAESPRIREVIISGGDPLTLDDRVLEKILASLKAIPH
                     VDVLRIGSRVPVVLPMRITRTLCRMLKRYRPLWFNTQFNHPAEITPESARACNMLQEA
                     GIPVSNQSVLLKGVNDSPDIMGDLLYGLQKMSVRPYYLFHCDPAQGCLHFRTEVPAGL
                     DMMEKIWRHCSGLCVPQYVLDVPGKVGKIPLNILSETMKNDLKKHQHFFDKRQEID"
BASE COUNT         2839 a         2642 c         2778 g         2945 t
ORIGIN      
        1 tgcaattacg gaagaacatt gatgcggttc cccatgccgt cggcgtgaat aatcatatgg
       61 gttccaagtt catgatggac gacgaaaaac tgtccgtcat ttttaaggaa ctccagcgca
      121 agaaactttt ttttatcgat tcgcgcacat cgccggatac ccgggcctat gccattgcgg
      181 acgacatggg tttccgtatc gctgaacgaa aaatttttat cgatcatcac cgtgattatc
      241 acgctattta cagtcaattg atgcatgttg cccagagcaa tgatcctgct cctaaaatta
      301 tcatcgggca tccgtatcct gaaacggtcc gggcccttca agcagcaacc aaagtcatgc
      361 gggaaaaggg ggtgtcggtt gttccggcat cccgaatcat taaaaaaaca aacaagtccc
      421 cggagaaatc ataatgccgg cgcatcatcg cgaaagcgcc gttaaaacgg attcgaagac
      481 cttatgaaga gaattttcat ttgtttcgtt atcattatgt tggtcagtct ttccggctgc
      541 gtgacgttgc cgaacgtcca gcatgctccg tcgggagata aatttcaaca gcaggcctct
      601 tatcattatc tgctgagtgt ttttctgcgc aatgatggaa atctggatga ggccatccgc
      661 gagctggagc aggcctatgc ggcggatccc gactccgcct atcttgtcat tgaactgatg
      721 tctctttacg cccagaaggg cgacgtcaaa agcgccgtgg cccatggcga agaggcgctg
      781 gcccgggatc cggacaatgt agacgtgcat ttgattatgg gcggcttgta cctgaatatg
      841 cgggagtatt cgaaagcagt cagagagcat aaaaaagtca tcgagctgga tccgaagaac
      901 ctcgttgcgc atctgtatct ggggattatt tatgcggaag aaaaaagatt cgacagagcg
      961 gaagagacat ttaaaaaaat gctgaaaatc gatccggata acatcatggg cgtctattat
     1021 tacgggaaag ttctggagaa gatgaaacgg ctggacgaag cggagaagat gtttaaaaaa
     1081 gccatgtccc tgaatccgta ttttgaaatg gccctgttcg atctggcggc tttgtacgag
     1141 aaacaggaaa aactggataa ggccattgaa atttaccgca attatctggc gattgatccg
     1201 acacggctta atttacgcgt gcggaccggc gagcttttaa tcaagaagaa aaaatatgaa
     1261 gaagcggaaa aggaatttca ggaagttttg aaaatcgatc cggataaccg ggatatcaaa
     1321 gtggcgctgg gtctgctgta ttttgacatg aaaaaatttg atctggccgc cgccgagttt
     1381 tcttcgattt tacagaaaaa tgtcctggac gacaaagtgc gttatttgct ggcgacaacg
     1441 tacgatcagc aaaaaaataa cgtccgggcc gttgaggaat ttacgggcat tgcatcatcg
     1501 tccgagctgt atgtcagtgc gcaactccgc gcggcgatga tcttgaaaga ggaggggaaa
     1561 gtcacggagg ccgtggcggt cataaaaaaa tccattaaga aaaagaacga tcaggtcccg
     1621 ttttatctgt tgttatcgtc cttttatgat gaaaccggtg ataacgcctc gtcggaagcc
     1681 attctgcagg acggtttgaa agtgctgcct cgaagcacgg atttgcatta tgccctgggc
     1741 gtgctttacg ataagaccaa ccgttttgaa gagagcatta tgtcgatgaa aaaaatcctg
     1801 gacattgatc cgaaaaatgc cgaagcgctg aatttcatcg gatacagtta tgcggtaagg
     1861 gacatgcatc ttgatgaagc ggaggccatg atcttgcggg cgctcaaaat caaacccggc
     1921 aatggttatt tactggacag cctgggatgg gtatatttca aaaaaaatga tttgaaaaac
     1981 gcggaaaaat atctgaaaga agcctggaca ctgctgcctg atgagattga aatcattgaa
     2041 cacctcgggg atttgtatgt ccggcagaac aggctcaagg acgcggccga tatgtatgag
     2101 cgggggatga aaatcgatcc cggaaacgat gtcctgaaaa agaagcgcga ggatatccgc
     2161 gggacacgat aatcaaacca caaggtcttt ccggataaga atcaactcat gatccaaaaa
     2221 tatttccgct tgatatggat gtttctgatg ctttgcctct gttcctgtgc gttacagcag
     2281 gcatcaggtg ttgcgcgcga tgtttatccg gaaaaaagga tcttgtcgac atccggcggg
     2341 cttgccgagg aggatgtttt ccaagccctg gcgaaaatag attttaaaac ggtcgacgca
     2401 tcgtatcatg tccgggccgc cctggttctg agacgtccgg gatacgtgcg cctggaagtg
     2461 ctgcccgtca tcggcaatcc cgatttgttg ctgacggcga cacccgatac catgactgtt
     2521 ttcgttccgt cgaaaaaaga gctttaccgg ggacgaccga cgcgttccaa tctggaaaaa
     2581 tttctaccct ggtcgatgga catcgaagat atggtcctgg tgttgacggg gacctatccg
     2641 ttttgcacga cagaacgtct tgccgaggat gtctcatccg aaggccgtct ttggcggatg
     2701 acatgcaaga aatcctcgag tgagtcgcaa atcatctggg tacaggaagg cagtcaaaaa
     2761 ctgatgcggc tgctttgcac cgatgaaacc ggttcggaaa aatataatgt gcaatatttt
     2821 tatggcggtc aaagcgcttt gcctgaaaaa cttgtgatcc gattggcgaa cggctcaacc
     2881 gcgctgaccg ttgcctactc cgatgtccgg gttgaaaaga cgaaagattt atcggttttt
     2941 aatctgaccg ttccatccga tgttgaacaa aatattttag aatagatcct tgcggagggt
     3001 gaaaaacgac tatttgatgt cctgcaaatt ggagcgcgcg atacgcgcct ggctggtgtt
     3061 cggataattt tttatgactt cctgcaggag taatttggcg ctggttttat cccccagttt
     3121 cagaaaggaa agaccctgtt tcaaaagagc ataggggacc ttgtttccct tcggataatt
     3181 tttggtgact ttttcatatt ccagaatggc cgattcatat tttttttcaa aaaaataaca
     3241 ttcaccaatc cagaactgtg cgttgtccga atattcgctg ttcggatagg tagtcaaaaa
     3301 agtctgaaat tcgtttcgcg ctttcgcata gtttccttcc tgaaaaatct gataggcggc
     3361 tgagtacgct ttatccttat cctgcttgct tccggtatcc tttgcgaccg agccgccatt
     3421 gactttgccc gttttatccg aggcgtccga cgtgtttttg ctgccgattt ccagaaaatt
     3481 ttcgatgaaa ttgattcgca gcaacatgtt attcagaagc tgttcgtctt tcctggacga
     3541 ctgcgacgcg tcttttttga gcgtttccac ctgaccccgc agttgctgga gattttccct
     3601 taattcggca agatccgcca tggtattggc ctgccctttg cgcatggatt ccagtgccgt
     3661 ggcgttttcc ttcaacgcgt tcacctctgc ttccagagag gatatttttt catccgttcg
     3721 ctggctcatt tccgaacgca gcgcccttaa atctccggag gaggcgcatc cggccagaaa
     3781 aaggaccacc agaaataaaa tgaaataacg aaatttttcc aaaggcgtca cctcgcttta
     3841 tcgttagggg ttgaccacaa aatgcgcccg tctgttcttg gcccaggcgt cttcattgga
     3901 ggccggatcc agaggatttt cttcgccaaa actgacggtt tgcatcaggg attctttcac
     3961 ccccagattc accagatatt tttttgtttc ctgagcgcgt ctttctccca gcgccatatt
     4021 gtattcgttg gtgccttttt catcacaatg cccttcaatg atgatggacg aaattctatt
     4081 gttcatgaaa tagtcggcat tttcccggag agtctcacga gcatccgggc gaatactgga
     4141 actgtcgaaa tcaaaatgga tatcgttgac aggggattgt gcccgcgcca ttttttccag
     4201 gtccaccgac ttttcctccg atgcaatctg ggacccggtg atatccttcg atgacatatc
     4261 ctcctgcgag ggggtcgtct gtgccggggt ggtttgctgt tcctgtgcgg catcgctggt
     4321 gataacggat tttttttcgg cgcaacccgc gatcatcatc aaaccggcta tgaatacgac
     4381 taaaacaagt cttaaatgga tttgcatgtt cattgcttca tctccttttt taaaggtgtt
     4441 caaaaattat attgatcaga aaatgacgac caagaatcgt tcacgttaaa accatgcaac
     4501 aaaacgctgt ttgtctgttt ttgactcccg tttcaccgaa atacattcta aatatgcgtg
     4561 ttttatacat caaagaaaag aaaataacaa cccctttgag atttaaattt gcctatcgga
     4621 gtcgtcccga ccatgccggc atggcgagtc tgttgctttg ctcatacagc agcctcggat
     4681 tggtcccgtt ggcattcatg atgaggattt gactgcttga atgttttcgg gacacataca
     4741 caatctgacg ccccgacggc gaccagaacg gcgactcatt gttggcgttg tcagaggtca
     4801 gttgcgccgg attgttgcct tccgtgtcaa cggaaaagat ttgatatcgg tcagcggtta
     4861 atccttcgta aatgatacgg tcgccgcgcg gtgaccagga gggcgaggaa ttataactgc
     4921 ctttgtaagt aattcttttc acgttgttcc catcggcatc catagtgtaa atttgcggcg
     4981 accctcctct attggaaaca aaaacaatct gcttaccgtc aggcgaccat gacggcgaaa
     5041 catcgatggc ggaatggcgg gtcaggcgct gaagctgtaa cgtctgaaca tccagggcat
     5101 atatttcttc gttgccgtcc ttgctcaggg tcattaatag ttttcgacca tcgggggaaa
     5161 aggaaccgca taaattcaat cctccaaaag atgccagtct tttttccgac ctgtttttta
     5221 aattgaggaa ataaatttca ggacgtcctc ttttgaatga ggtaaaggcc attccgtttc
     5281 cgtcaggcga ccagcgcggc gccattaaga tggactgatg atgggtgact ttcctgacat
     5341 cggcgccgtc ataactgacg gcataaatat cagaccggag gcctgtcttg gatacaaagg
     5401 ctatttcggt gttgaagtct cccgcgtcat tgatcagggc aagcatgatg tcggatgcta
     5461 tcgcgcgggc gaccgccttg gttttttcgg ccggcgcctg gtattttttg ccgaaaatca
     5521 actcaccgcg ggcgacatcg taaagatagg cctcgacggt aatttcccga tccttctgaa
     5581 agatgcgtcc tttgaccaga tagtcggcgc ctacggccag ccagtcggca aaacgaattt
     5641 tccccacggc tgttgtcgat acggcatcag ggttttccag aaaactttgt ttgttcaaaa
     5701 tattgaacag tcctgtcagg gtgagatctt ttttaacccg ttcggaaacc gttgtctcgg
     5761 aagcgacatg gccgaccgga cccgacgcag gatgaaaatc gcagatggcg atgggaatca
     5821 gtctgaaagc aggagaatcg atgtccacat acactttgga atctgcgcgg ttgctgcaga
     5881 ttggccaggc gcagaaaaaa aagatcaacg ttaaaatcaa aactcttttt gtcacgggat
     5941 atccctttct ctttctttaa aaagcgttct cctgcacacg tgaggtcagc gcagttccga
     6001 ggaatgaaac cggatgccga tttcaatatg gttatccctg atccaatggg gcaacggagg
     6061 aaacggaccg gattttttga ccgctctgag cgcggagtca tcgaaataac ggttgccgga
     6121 gcgtttttca aaaccggcat acaacagggc gccgttgcgg gcaatcctga catccacaat
     6181 cgtcgtgata ttgttatccg gcagcaggga ttgcggtatg gaccagttct gctttaccct
     6241 cgaccacacg gtttcgatat attcattggc ctgcgcattg acttcggcgt ccgtcatcgc
     6301 cgttcggccg gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagatca
     6421 tgttttgaaa gttgccgtta ccgctgcggt agcccttgcc aagcatccgg ggtgtcattt
     6481 tgtttcctcg ggaggttccg tgatcattcc cagtttatca acccctgctt tacgaacttc
     6541 cgacatgact tcaaccacaa agccataagg cacatttttg tcggcgcgca gaaaaatttc
     6601 ccggtcgatt ctgttggaaa agatggcttc cagtttgggt tttaattccc ccagcgtcat
     6661 tttggtttta ttcagaaaga tttcccgggt ttcattgacg gtcagaacca gtttttcttc
     6721 ggccacatcg acgcttttgg ctttaacccg cggcagattg acatcgatgc ccatcgacag
     6781 catgggcgcc gtcaccatga agataatcag cagcaccagc atgacatcga ccagaggcgt
     6841 aacgttgatt tccgccagcg gtttactgga attatttttt ctgcgtgacc cgcgcaccat
     6901 tgtttttatc tccgggattt atttacggac aagataacgt tccacaatgt ttaagaattc
     6961 agcggaaaag ttttccattt cctgaaccat gtttttcgac tgatttaaaa aataattata
     7021 gaagataacc gctggtatcg ctgcagccag tccggctgcg gtggcgatta atgcttcgga
     7081 aattccggga gcgacaacag ccagcgtggc cgacccgcgc gcgccgatgc ctttaaacgt
     7141 atccataatt ccccagacgg tgccgaacag accgataaaa gggctggccg acccggttgt
     7201 agccagaaat cccagcgaac tttccagctt ggtcatttct gtggcactgg ctttattcat
     7261 cgagcgttcg agattatcca gtgacgaaag actgatgtcg tcgcgtccct gggccgattc
     7321 acctgcggcc ggtctgttgg tctgggtcag ttccttgtac cccacgcgaa aaacttccgc
     7381 cgtggtggag tatgaatatt ttttcgctgc cggcaagaca tccgaaagtt tcgaactctt
     7441 cagatagtcg tcattaaacg cttcattttc tttttgaatt ttgcgataat agcgatactt
     7501 gatgaaaatg atcgcccagg aaaccacaga aaaaatgaaa agcaccagca aaacaaactt
     7561 aaccatcagc ccggcatcga gaatcatgct caaaaggctg ccgttcacac tgccgcttaa
     7621 gtctaaagct ccaaagtctt tcacttttaa tctccaatgg gaaatgaatt tgttgcgtaa
     7681 gctaaaggaa aaaaaagtcc ttgtcaatat atcttttaac gtaaaaaact tcttgttttt
     7741 ttttagaaat gatagtagat gtaccgataa cttaaaaact atttgattaa aacgtaataa
     7801 taataagggg gacctatgaa aagaaaaagg attttaactc aatttttgtt atattttctg
     7861 atgttgatgt ttttgatagt cgccggttgc agcagcggcg gcagcgatga cgatgatgat
     7921 gatcaaaatc agcaacagtt gtttgtgact gtttcgggta aggtaaccga agcagacggg
     7981 attaccgggg tacagggagt caatatttca gccatgtact cattgacgat cctgaatgtc
     8041 aaggtctggg agggctcgat cagcagtgat ttgttcagct atattatcac ggatgccaac
     8101 ggcaattaca cattgaggct gcccgcagcg ctcgtcgaac agtataacgt tattcttaca
     8161 cccggtaaaa agggttacac cttcaccccc gccagaagaa ctttcgatgt aggtttgacc
     8221 gatataacag gcgtgaactt cacggctgcg gaagcatccg tatacggtca ggccgacctg
     8281 gccggaacgt ggcgtactaa tatgcttcga acgggcaccg aaaatgggtg ggtgcgtgcc
     8341 cgcttgacca tcggcgccac cggtgttgcc acgtgtgagt cgtatgaaac cagcacgtca
     8401 gcgggcgata caacctgtcc ggccgatttt gatttaacat ttaccgtcaa tccagatacg
     8461 ggtgcgatca ttcaaagcgg tgcctatgct gcgggagacg gcggtcacat gaccatgaac
     8521 tccgatatgg attttgctgc cggcacggca accgcaggaa caaactacca gatcatgatc
     8581 gcgcagaaag aagggaccac caattacagc aatgccaatc tggcaagtca taacttcgtc
     8641 gttcatggca tggttgtcgg tcaaaccaat gagtggtggt atggaacagg agcaaccgat
     8701 accgctctgg taacaacgat gagcgaagaa gtaggtccca caggtgctcc ttctgcaacc
     8761 atcaatggcg taacgttagc cgtagcggtt gacggtgttg tgaccctggt ggatgcttta
     8821 ggggcaagag ccagtgattt cgaaggattt ctctccgtcg ataaaaagac aatggttgga
     8881 acctataccg atattaatgg cgattatcag ttaatgatcg ttcagattac cgatggacag
     8941 gctgtggcca atttgaccgg atcgtcctat aaccatctgc tgggtgtcgg cgatgacgtc
     9001 ttctgggcgc atcatgatat taatgtaagc cggtttgaga cgtccttcct gccggatata
     9061 ttggcgctta ccttcaattt ggatgtcatg ctgtcctaca actggaattt gtctctcgct
     9121 tatacaccga tacagaagtt gggtctggtc agtgtcaaga aactgaccat tgctcccaac
     9181 ggtcaggcaa tcgtcacgaa tcctgaaacc gatgcaattg ttttccatgg acagctggca
     9241 tttgacggta cgttcatggt tggtacggaa acaatggagt tgacagaagg cactttctat
     9301 acactgaatg tcatcaccca ttaatcacta aaaaccagat aaacaaaaac ccgcccctta
     9361 ttcggggggc gggttttttt atgcattaat ttcttaacaa cgatggatca aagattttcc
     9421 ggtataaacg caaccacatc atcaatgcag tttgcaccgg ttatgatcat caccagccgg
     9481 tcaatgccca gcgcaatgcc ggcacagggc ggcaggtgtc ccagggactg aagaaatttc
     9541 tccggcatca gatactccga ccattttttt tcagcccgtt ttttcagcgc ctcttcaaaa
     9601 cgttttcttt gttccaccgc atcggtcagt tctgaaaagc cgttggcaat ttccagcccg
     9661 ccgatgtaaa gctcgaatct ttcgctgacg gtgcgatgat tgtctttaag tttcgccagc
     9721 gccgccagtg aggccggata gtcataaaga aacgtcggtt tgccgattcc cagccggggc
     9781 tcgatacagt caacaaggat ttcatcgaac gtgccctgcc ccagagcctc atccagggta
     9841 agcggcgcgt atcttaaaaa ggcgtcttcc acggtgatgc tctcccacgg cggtgttaag
     9901 gcaatggtcc ggttgtctct tttgatttca gtaaggccct gattttgtgc aaccgcaacg
     9961 atcaggttcc ggcattgggc catgagttgc cgataatcga atccggcgac ataccactcc
    10021 agcatcgtaa actccggcag atgcagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnggc aggctgatgc cgatgggccg gtcttcaaaa agaagaattt ccacttcgat
    10201 gttatccgtc ataaaattta cggcatcatc gacctgttct tccgtcagat aaacctgctc
    10261 gaaggtttca ttgtccatga agcagaattt gtctccttcc ttatatagat actgcatggt
    10321 cttttcccgt aaatccgcct gttcgaatgt atctacagaa cgaaatgttc gttcaaactg
    10381 attgccgttg atcatgtttt tgagtttggt gcgatacagg gcctgtcctt taccgggctt
    10441 gacgaaattg aattcaacga tgacataagg ttcgccatcg attttcagcc gcagtccttt
    10501 tcttaaatca ctggcagtgt acataaattt tctccttgct tagtgtggca aaatcgcctg
    10561 ttttgcctca ggcgcttctc taatctattt cttgacgctt gtcaaaaaaa tgttgatgtt
    10621 tctttaaatc atttttcatc gtttcggaca ggatgtttaa ggggattttt ccgaccttgc
    10681 cggggacatc cagaacatat tgcggcacgc aaagtccgga acagtgccgc catattttct
    10741 ccatcatatc aaggccggcc ggtacctcgg tgcgaaaatg cagacaccct tgtgccgggt
    10801 cgcaatggaa aagataatag gggcggacgg acattttttg cagaccgtaa agaagatcac
    10861 ccatgatgtc cggggaatcg ttgacgcctt tgagcagaac cgactggttg gagacgggaa
    10921 tgcccgcctc ctgcagcata ttacaggccc gcgccgactc cggtgtaatt tccgcggggt
    10981 gattgaactg cgtattgaac cacaggggcc ggtatcgttt gagcatccgg cagagcgtcc
    11041 gggtgatgcg catcggcaga acaacaggca cgcggctgcc gatacgcaga acgtcaacat
    11101 gcgggatggc tttcagagaa gcaaggattt tttcgagaac acggtcatcc agggtcaggg
    11161 gatcgccgcc ggaaataatc acttcccgga tgcggggcga ttcggcaatg taacgcgtta
    11221 tcttctgcag acggtcagtc aggttcaggg aagcggggcg ggaccagaaa cgctttcggt
    11281 tacagtgccg gcagtacacc gcgcaggttt ccgtgacaat cgccagacaa cggtccggat
    11341 agcggtgcac cagtttggga accggcatat gcaaatcttc ctgaagggga tcttccgccg
    11401 cccg
//
LOCUS       DAONQZ010000072        11245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_6364_length_11245_cov_16.029580, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000072 DAONQZ010000000
VERSION     DAONQZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_11245_cov_16.029580"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PLR13_09755"
     CDS             <1..1430
                     /locus_tag="PLR13_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HQC19665.1"
                     /translation="MASVISIEYFRAKKSALEGDAEAQDTLGAMYMRGEGVERNQEEA
                     IKWFTKAAEQGDIHAKISLGYAYVDRKGVETNIEKGLALLLEGAEHGDWFAQLIAGSI
                     YLGMYGGEADYKEALKWLTKSAEQGNVTAQFFVGKMHFRGDGTEKNYTEAAKWLKKAV
                     AQGNIEARTYLAGLYLEGKGVSRDSGEALRLFRKSALQDDKFAQYGLALLYYEGIEAE
                     KDLKKAAYWFRKSAEKGCVDAECDLGLAYFHGDGVKKDYGEALKWLRKAAEKEFARAE
                     HQLGIMYHEGTGVEKDYSEAIKWFRKAAEQGYSRSQLELGLMHYAGEGMEIDYHEARK
                     WFEKAAVQGNDSAQLCMGLIYLKGQGIDKNYVEAMTWFKKSAANGNSRAQYNIGVMYF
                     RGEGIERDYKEAFRWTKMAAEQGDDKAQDLLALMYVGGRGVEKNYEKGMEWFKKSAAQ
                     GNKKAIETIEHFQNIRKEMLKKQKA"
     gene            1833..2606
                     /gene="cas6"
                     /locus_tag="PLR13_09760"
     CDS             1833..2606
                     /gene="cas6"
                     /locus_tag="PLR13_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HQC19666.1"
                     /translation="MRFSAEILLIGEKLIVPSDYRRNILSLIKEAINSSQSGVEIYEK
                     YYVKEARKIKPFTFSINFKIDDTYSEKGSLHLAESKLNLFFSASDPVFLIYVYNGLVQ
                     LKKDYPLFPGLKAKVGRFKLEQQKNFRGSEAIFKTCSPILVRNTDASGKSRGYITADT
                     PEFEDKLYHSAKSMCKVLLDGAPELKRNDFAAEWIRCRNVHINHYKNKDASQDSKKEL
                     IVATTGTIKIKAPANVLQLIYDAGIGAKRSQGFGMMEVL"
     gene            2823..3890
                     /locus_tag="PLR13_09765"
     CDS             2823..3890
                     /locus_tag="PLR13_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19667.1"
                     /translation="MSDDDFIKLYPTNWLYNSAVLGFLSVLHKNGYDINSFLSDDGTI
                     YFPNKMIIAESINPYKKFNEMNKQKPIPLVGKNANCPNYLQKGEEAAFEKLINDFVHL
                     EENHSMQCSLCTQHFHLADNPSWSVDVKKVKERIVDSGFTVIHSSSLAPSIKKFPNSL
                     WNMKASMNLCPLCSYLLVFSHIAFTHTIEGAILINGTSFKTMWHLNKYAAEVFSRQNV
                     RNIFAMSLMEYSQKVNATLGMWNIANNEVVIKTRDGRIDHYSMPYEQTRLLLNRKISA
                     LITKTREPYVLDAVLSGHFVDLLTMNEKLLKAVQSNNVDDGKRYLCNYENHKTSRMRT
                     IAATLPELYIQINKQLHEEVI"
     gene            3890..4441
                     /locus_tag="PLR13_09770"
     CDS             3890..4441
                     /locus_tag="PLR13_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19668.1"
                     /translation="MEQSTAKQIEYLIDQLYGKIMPESFDQWHDDVRDVIKIFSRKDR
                     IRDFVDSFEAVKRAIAKEPMENDEKKKKLAKLTRLIALLKSSEPDFLLESLQRKGFAI
                     AKTDSELRGSIENSIFRILQQIRLGKKAAVCGLFMQIFSARGKAMPQELVDALKEEWD
                     INQYRAFMYAFLSNFTGREEYND"
     gene            4434..5819
                     /gene="cas7i"
                     /locus_tag="PLR13_09775"
     CDS             4434..5819
                     /gene="cas7i"
                     /locus_tag="PLR13_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HQC19669.1"
                     /translation="MTSNNTIAWETIKGLTFTIIFEGSALNRDEKLGGNIPSIKKLTR
                     YANGRRGKTFSFISRESMRHHLFVTLNKNYPVQWQPAPTTAINDVVQFDLTRANILTH
                     AELDAFGYMFTQSKSAAITRKGAIGLQKAVSLESWEGDMQFNANHDIASRCGAYPDPV
                     NKEEHSSYYKTTFTIDIDKLGCDEWKIVNYEAVNNKLNLYMTEKGQEVFIANVEVREN
                     DEGIEEYFLGKEKITFSGNSICVSKKLMKIDAKKKHILFDSKILKKEDAAQGKGKKPT
                     FAVPETDYTEEDGCYIFSVASFSYNKDDKSIKVSRVMSFSFEYHRESEGKYMLTSGKN
                     SHIEIAENGKTAKFILDPSDKKERVRNIIHSIKNGMYFHTSGENTGIIPKYIVGAALT
                     LPIPLFHSYVDLTKFNDSILGNDYILKGAVFCQNHMGSDTFDVKEAETNLDTFLNKIG
                     LGEARDGKTNP"
     gene            5800..6627
                     /gene="cas5b"
                     /locus_tag="PLR13_09780"
     CDS             5800..6627
                     /gene="cas5b"
                     /locus_tag="PLR13_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HQC19670.1"
                     /translation="MEKQILRVKLYQSSAHYRIPFSYQRRFTYPIPPLATVKGLLCNL
                     MGIKADTSQEYKSLFSGLSVAVYGRFESIVKDYTWFRNLSKESHISKFQTTTNRIHDF
                     APQHPGGQMPVSVDSLNENRVIIYFGHCDNEKLLEIEKCFRNPEKRNSTVHLGRAEDW
                     LVVEDIRQITTEEKAVRRGRFFFWLPEPSYCDESYLRGTPSQYEAFFNKAPGNCLRMP
                     LFYRITEGNERIFETYYNAKLYEGGVFPEMCTFLIDRLDDETELPLIFTKVRLQNGI"
     gene            6617..9169
                     /gene="cas3"
                     /locus_tag="PLR13_09785"
     CDS             6617..9169
                     /gene="cas3"
                     /locus_tag="PLR13_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HQC19671.1"
                     /translation="MVSEIVAKSHKDRNKSSITLRDHTSELLNQVESIKKQLQTLPAY
                     HKVPWQTLEHAVALHDLGKASPSFQVNSVGNWIYSPKALFPTVPHSILSIFFIVPDAL
                     PQISADEKRLLLSAIAFHHWRDNFSDIISGNSKELQEAIGKISGNNNLRNGLLGNLLN
                     AFQTHPLLKKYTTAIGFNNDLATMIEAGGDLFNYITPPYLSYFLPQRIESSNDFKRNY
                     IFVAGFLMRTDHFSSYVQETGINETIERKCLSKDDAANNVKAYIKSKFPQTDLTALWQ
                     IQELNNKSNDNLVVVAPTGSGKTELAALWGAGHKLFFTLPLRSAVNANYERMMSYFGP
                     NDVGLLHSDADVYVYEKTGHISDENMRTLDMARLFALPVQVSTGDQIFPAALKYPGYE
                     KVHATLAYSRLVIDEVQAYDPRSAAIIVKMIEDVFTLGGKFMLMTATLPDFIYTELQK
                     RLGQENYQFIDRYSGLDDLTKHEITLHNTDIGNAIEDIGKTAMEGKRVLVIANTVKKA
                     QSIYEKLKDTKNIKIKLLHSRFTLKDRADIENEMINKEFNNPKPPEEREAKILIATQV
                     VEASLDIDADVLFTEIAPMDSLVQRMGRVMRRYKTASEVTEDGKVNIFYSYKLRDKGF
                     EFESGSGYVYDNDLLILTIAELNGQDAEEYLTLKYSKDTKKVEKKSKKSVNDILQSIL
                     EHECISKPISEQKKKEMVGNVFNKLSNDSRYKKRFYEMLSVLDAGYISDRKADAARLF
                     REIFTIPVIVFKDANEFIKSVTDICLSGDCEWVRFKKDVLSRFTIHVSKYAIDISSSA
                     STSDLQYDPSIDKDKMKKILRWTEDIRIIKDGKYDDKLGFIPDPKKDWRWDM"
     gene            9157..9666
                     /gene="cas4"
                     /locus_tag="PLR13_09790"
     CDS             9157..9666
                     /gene="cas4"
                     /locus_tag="PLR13_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HQC19672.1"
                     /translation="MGHVNAEHPNITGTQVNYYLICHRKLWLFSHNISMEHTSEAVEI
                     GSLVHEHTYERKNKELVFDGIKIDFFDKNHGIIHEVKKSKKMEEAHFWQLKYYIYHFK
                     KLGLSVSGKIDYPLIRRTQTVELTAEDEAQLERTLDDIVTINERELPPPVIKAKICKS
                     CSYYELCYV"
     gene            9737..10726
                     /gene="cas1b"
                     /locus_tag="PLR13_09795"
     CDS             9737..10726
                     /gene="cas1b"
                     /locus_tag="PLR13_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HQC19673.1"
                     /translation="MRDYYIFKSGRVSRKDNSVMFEYTEDGQDRHVPIPINDIDALYL
                     FGEVDLNTKMLNFFAQSNVMVHFFNYYGYYTGTFYPREFLNSGDVIVKQVASYLRKSV
                     RLELAKEFVKGAAYGIIQNLKRYKEKTSGELEKVETYFADIDKQSTIAQVMGLEGNIK
                     ETYYAAFPKIINQDIEFEKRVKNPPNNMMNALISFVNSMIYTEMLREIYKTQLNPTIS
                     YLHEPGYRRFSLSLDIAEIFKPIYGDRIIFALLNNNELQKKHFDKDVNYAYLIEEGRK
                     IVVKAIDEKLNTTIMHKQLKQQVSYRKIMRLELYKLVKHVLGESKYQSFRIWW"
     gene            10730..11011
                     /gene="cas2"
                     /locus_tag="PLR13_09800"
     CDS             10730..11011
                     /gene="cas2"
                     /locus_tag="PLR13_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQC19674.1"
                     /translation="MYVIAVYDISTIEPAGQRRLAQIMKKMRQYLHHTQKSVFEGEIS
                     VAKLMSLQKEAKNIINEETDYVVFFRIGNKKNVERECIGIQLDPTDVII"
BASE COUNT         3685 a         2100 c         2502 g         2958 t
ORIGIN      
        1 ggatggcttc tgtaatcagt atagaatatt ttagagctaa aaaatcagcc ttagagggag
       61 acgccgaagc gcaggatact ctcggcgcca tgtatatgcg cggcgaaggt gtagaaagga
      121 atcaagagga agcgataaaa tggttcacaa aggccgcaga gcagggagat attcatgcca
      181 aaataagctt ggggtatgct tatgttgata ggaaaggcgt tgaaaccaat atcgaaaaag
      241 gactggcttt gcttttagaa ggcgccgagc atggtgattg gttcgcgcaa ctgattgccg
      301 gctctattta tcttgggatg tatggtggtg aagcggatta taaagaagcg ctcaagtggc
      361 tgacaaaatc agcagaacag ggaaatgtaa cggcacagtt ttttgtaggc aaaatgcatt
      421 ttcggggaga cgggacagaa aaaaattata ccgaagcggc aaagtggctt aaaaaagccg
      481 tcgcgcaggg caatatcgaa gcacgaacat atcttgccgg tctttatttg gaaggcaagg
      541 gcgtttccag agatagcggt gaagctttaa ggttgttcag aaagtctgcc ctgcaagacg
      601 acaaatttgc ccaatatggc cttgccctgc tgtattatga aggcattgag gcggaaaagg
      661 acctcaaaaa agccgcatat tggtttagaa aatcagcgga gaaaggctgt gtcgatgccg
      721 aatgtgatct tggtttggct tattttcatg gtgatggagt gaaaaaggat tacggtgaag
      781 cgctgaaatg gttaagaaaa gccgcggaga aagagtttgc ccgcgccgag catcagcttg
      841 gcataatgta tcacgaaggc acgggtgtag aaaaagatta cagcgaggcg attaagtggt
      901 tcagaaaagc cgcggagcaa ggatattcac gctcgcaatt agaattaggg ctaatgcatt
      961 atgcgggcga aggtatggaa atagattacc atgaagcgcg aaaatggttt gaaaaagcgg
     1021 cggtgcaggg gaacgattcc gcgcagcttt gtatggggtt aatatatttg aagggtcagg
     1081 ggatagataa gaattatgtc gaagcaatga cgtggttcaa aaaatccgcg gctaacggta
     1141 acagtcgtgc acaatataat atcggcgtaa tgtatttccg tggcgagggt attgaaagag
     1201 attataaaga agcattcagg tggactaaaa tggccgcgga acaaggagat gataaagcgc
     1261 aggatcttct tgctttaatg tatgttggtg gtcgcggcgt tgaaaaaaat tatgaaaagg
     1321 ggatggagtg gtttaaaaaa tctgcggcgc agggtaacaa aaaagcgata gaaactatag
     1381 agcattttca gaacataaga aaagaaatgc tcaaaaagca gaaagcgtaa atgctttgtt
     1441 ttgtcattgc cctcttgcct gccccgcgaa tgtggggtca ttgcgagacg gcacgcgccg
     1501 tcgtggcaat ctctttcccc tttggccttc ggccttattg gggttgacac agcaaaaacc
     1561 ggcaggtata atcttttccc gtaattaaaa gctccaaata ccgaaagtct caactggaaa
     1621 cagtaaaata ataactcgat cgccgacgcc gattaatgcc gtgtacgcat gtctttgcat
     1681 agttgaatta tcgacaagct aacgttaaag attacttacg accaataaac aaaagaaatt
     1741 actagggttt cgatattttt aaaataattt agacggaact gcgctttagc ttatagatag
     1801 tcttataatt taataattat aggagaatgt ttatgagatt ttctgctgaa attttgttaa
     1861 ttggcgaaaa actgattgtt ccatctgatt atcgtcgaaa catcctttcc ctaatcaaag
     1921 aagcaattaa ttccagtcaa agcggtgttg aaatatatga aaaatattac gtaaaagagg
     1981 ctaggaagat aaagccgttc acattttcga tcaattttaa aattgacgat acttacagcg
     2041 aaaaaggaag tttgcacctt gctgaatcga aactgaacct gtttttttcg gcaagcgatc
     2101 ccgtctttct catttacgtt tacaacggct tggttcagtt gaagaaagat tatccgcttt
     2161 tccccggact taaagcgaaa gttggacgct tcaaactgga acaacaaaag aactttcgcg
     2221 gcagcgaagc catctttaaa acctgctcgc caattctcgt aaggaacacg gacgcatcag
     2281 gcaaatcccg gggctatatc acggctgaca cgccggaatt tgaagacaag ctttaccatt
     2341 ccgcaaaatc catgtgcaaa gtattactgg atggtgcacc cgaactgaaa cggaacgact
     2401 ttgccgctga atggatacgt tgccggaacg tacacattaa tcactataag aacaaagacg
     2461 cttctcagga cagcaaaaag gaactgatcg tagccacaac aggcactatc aaaatcaaag
     2521 ctccggccaa cgttttgcaa ctgatctacg acgcaggaat cggcgcaaag cgcagccagg
     2581 gatttggcat gatggaggtc ctttaatgaa gaaaaaagaa acggcaaaca atcaaatgca
     2641 cttgcgaaaa aagaatatga aggtttcaga attgaacagg acaaatactt tgaatccgat
     2701 tttggcaaac tggttaagaa gtacttgccc aaaacgaaaa aaagaaactg tgaagggaac
     2761 tacatcaaag ataaattcaa gcgtatcggc ccggacaaag tcggccgctg ggaggttgtg
     2821 gaatgagtga tgacgatttc ataaagttgt atccaacaaa ttggctatat aattcagcag
     2881 tgcttggatt cttaagcgtc cttcacaaaa atggatatga tattaattca tttctttcag
     2941 atgatgggac aatttatttt ccaaacaaga tgattattgc tgagagcatc aatccatata
     3001 agaaatttaa tgaaatgaat aaacaaaaac ctattccgct tgttggcaag aatgcaaatt
     3061 gtccaaacta tctccaaaaa ggcgaggaag cggcttttga aaaacttata aatgattttg
     3121 tccaccttga agaaaatcat tcaatgcaat gctccctatg cacccaacat tttcatttag
     3181 cagataatcc aagctggtca gtagatgtta agaaagttaa ggaaagaatt gttgattctg
     3241 gatttactgt tatacattca tcttcgttgg ccccttcaat aaagaaattt cctaattcgc
     3301 tttggaatat gaaagcaagt atgaacttat gtccattgtg ctcatatcta ctcgtttttt
     3361 ctcatattgc ttttacgcat accatagagg gcgcaatatt aataaatggc acatcgttca
     3421 aaacaatgtg gcatctcaat aaatatgctg cggaagtatt tagcaggcag aatgtacgta
     3481 atatatttgc catgagtctt atggaatatt cacagaaagt taatgcaact ctgggcatgt
     3541 ggaatattgc aaataatgaa gttgttatta agacgcgcga tggtcgcata gatcactatt
     3601 ccatgcctta tgagcaaaca cgacttttgt taaatcgtaa aatttcggca ctgataacaa
     3661 aaacgagaga gccatacgtt ctcgacgctg tgctttcggg gcattttgtt gatcttttaa
     3721 caatgaatga aaagcttttg aaggcagtcc agtccaataa cgtagacgat ggtaaaagat
     3781 acctttgcaa ctatgaaaat cataaaacaa gtcggatgag aacaatagca gcaaccttac
     3841 ctgaacttta cattcaaata aataaacaac ttcatgagga ggtgatctga tggaacaatc
     3901 gacagcaaag cagatagaat atctgattga ccaactttat ggcaagatta tgcctgagag
     3961 ttttgaccaa tggcacgatg atgtacgcga tgtgataaaa atatttagca gaaaggatcg
     4021 tattcgagat tttgttgata gctttgaagc ggtaaagcgc gccatcgcaa aagagccaat
     4081 ggaaaacgat gaaaaaaaga aaaagctcgc gaaactaacc cgccttatcg cgctgcttaa
     4141 atcaagcgaa cccgattttt tgttagagtc tttacagcga aaaggttttg ccatagctaa
     4201 gaccgattcg gaacttcgtg gcagtattga gaattcaatt tttcgcatat tacagcaaat
     4261 ccgcctcggc aaaaaagcgg ctgtctgcgg tttgtttatg caaatatttt cggcaagagg
     4321 caaggcaatg ccccaggaac ttgtcgatgc attgaaggaa gaatgggata ttaatcaata
     4381 cagggcattt atgtacgcct ttttgagcaa ctttaccgga agggaggaat acaatgacta
     4441 gcaataatac aatcgcatgg gagacaataa agggactaac attcactatc atttttgagg
     4501 gatcagcgct taaccgcgat gaaaagcttg gcgggaatat cccatcaatt aaaaaattaa
     4561 cacgatatgc aaatggacgt agaggtaaga cttttagttt tatttcacgg gaatccatgc
     4621 gacatcactt atttgtgaca cttaataaaa actatcccgt tcaatggcag ccggcaccga
     4681 caacagcaat aaatgatgtc gtccaatttg atttaacaag agcaaatatt ctgacacatg
     4741 ctgagctgga tgcttttggc tacatgttta ctcaatcaaa aagcgcagca ataacccgta
     4801 aaggtgccat cggattacaa aaggcagttt ctctcgaatc atgggaaggc gatatgcaat
     4861 ttaatgccaa tcacgacatt gccagtagat gtggtgcata tcccgatcca gtgaacaagg
     4921 aagagcattc atcatattat aaaacaacat tcacaataga tattgacaag ctgggatgtg
     4981 atgagtggaa aattgtaaat tatgaggccg ttaataataa attaaatcta tatatgaccg
     5041 aaaaaggtca agaagttttt atagccaatg tggaagtcag agaaaacgat gaagggatag
     5101 aagaatattt tttaggtaaa gaaaaaatca cgttttctgg aaattctatt tgcgtatcta
     5161 aaaagctcat gaaaattgat gcaaagaaaa aacatatttt atttgattca aaaatattga
     5221 aaaaagaaga tgctgcgcag ggcaaaggta aaaaaccaac atttgcagtt cctgaaactg
     5281 actacacaga agaagatggc tgttatattt tttctgttgc cagtttcagc tacaataagg
     5341 atgataaatc aataaaagta tcgagagtca tgtcgttctc atttgaatat catcgggaat
     5401 cagaaggcaa atacatgctc acctctggca aaaatagcca tatagaaatt gctgaaaatg
     5461 ggaaaactgc caaattcata ctcgatccaa gtgataaaaa agagcgtgta agaaatatta
     5521 tccattcgat aaagaatgga atgtattttc ataccagtgg ggaaaatacg ggcataattc
     5581 ctaagtatat tgttggcgct gcccttacct tgccgattcc tttatttcat tcgtatgttg
     5641 atttgactaa gttcaatgat tctatcttgg gaaatgacta tatcctaaaa ggcgcggtct
     5701 tttgtcaaaa ccacatgggg tctgatactt ttgatgtcaa agaagcagaa actaatttgg
     5761 atacttttct aaacaagatc ggcctgggag aggcaagaga tggaaaaaca aatccttagg
     5821 gtaaaattat atcagtcatc ggcgcattat aggattccat tcagctacca gcggcggttc
     5881 acgtacccca taccgccatt ggcaaccgtc aagggcttgc tatgcaattt aatgggaatc
     5941 aaggccgaca cttcgcagga atataaaagt cttttttcgg gtttatctgt tgccgtttat
     6001 gggcggttcg aaagcattgt gaaagattac acatggttta ggaatttatc caaagagagc
     6061 cacattagta aattccagac aactactaat cgaatccatg acttcgcacc acagcatccc
     6121 gggggacaga tgcctgtcag tgttgacagc ctgaacgaaa acagagtcat catttacttt
     6181 ggccactgcg acaacgaaaa acttcttgaa attgaaaaat gctttcgaaa tccggaaaaa
     6241 agaaacagta cggtgcatct tggtcgggcg gaggattggc tggtcgttga agatatcaga
     6301 caaataacaa cggaagaaaa agctgtccgc agaggccgtt tttttttctg gcttccggaa
     6361 ccgtcctatt gcgatgaatc ttatcttaga ggcacaccct cccaatatga ggcatttttc
     6421 aataaggcac cgggcaattg tctgcgaatg cctctttttt accgcattac cgagggcaat
     6481 gaacgaatat ttgaaaccta ctacaacgcg aaactatatg aaggcggtgt tttcccagaa
     6541 atgtgcacat ttcttattga taggcttgat gatgaaactg aactgccgct tattttcaca
     6601 aaagtgaggc ttcaaaatgg tatctgaaat cgttgcaaaa agtcataaag atagaaataa
     6661 aagcagcata accctgagag accacacgtc agaacttctt aatcaagttg aatcgatcaa
     6721 gaagcaatta cagacattgc ccgcttacca caaggttccg tggcaaactt tggagcatgc
     6781 cgttgcgctt catgatttag gaaaagcatc gccatctttt caggtcaaca gtgtaggtaa
     6841 ttggatctat tcccctaaag cactatttcc gactgtgcca cacagtattt tatcaatttt
     6901 ctttatcgtc cctgatgcac taccacagat atccgcagat gaaaaacgtt tactgctttc
     6961 agcgattgcg ttccaccact ggcgtgataa tttcagtgat atcatttccg gcaacagtaa
     7021 agaacttcag gaagccattg gtaaaataag cggtaataat aatttaagga atggacttct
     7081 tggaaacctg ttaaatgctt ttcaaacgca tccccttctt aaaaaatata caaccgctat
     7141 tggtttcaat aatgatctgg caacaatgat tgaggcgggc ggcgacctgt tcaattatat
     7201 aacaccgcct tatcttagct atttccttcc acaacgaatt gaaagcagca acgattttaa
     7261 acgcaattat atctttgtgg caggcttcct gatgcgcacc gaccattttt catcctatgt
     7321 tcaggaaact ggtataaatg aaaccataga gcgtaagtgt ttatcgaagg acgatgcggc
     7381 caataatgtc aaggcgtata ttaaaagcaa gtttccccaa acagacctga cggcattgtg
     7441 gcagatacag gagctcaata ataaaagtaa tgataatctc gttgttgtcg ccccaaccgg
     7501 ttccggtaag accgaacttg ccgctttgtg gggtgctggg cataaattat ttttcacgct
     7561 accacttcga agcgctgtca atgcaaacta tgaacggatg atgagttatt ttggcccaaa
     7621 tgacgttggc cttttacatt ccgatgctga tgtttatgtc tatgaaaaga ccggccatat
     7681 atctgatgag aacatgcgta cgcttgatat ggcaagattg tttgcgctgc cggtacaagt
     7741 atcaacaggc gatcaaatat ttcctgcagc tttgaagtat cctggatacg aaaaagttca
     7801 cgcgacactt gcctattcac gccttgtaat tgatgaggtg caggcatacg acccgcgatc
     7861 agcggcaatt atcgtgaaga tgattgaaga tgtcttcacg ctcggcggca aatttatgct
     7921 catgacagca accttgcctg attttattta cacagagcta cagaaacgtt taggtcaaga
     7981 gaactatcag tttatagaca gatacagcgg gcttgatgac cttacaaagc atgagataac
     8041 gctgcataat acagatattg gaaatgcaat tgaagatatt ggaaagacag caatggaagg
     8101 aaaacgtgtg ctggttatcg ctaatacggt caaaaaagca cagtcgattt acgaaaaatt
     8161 aaaagacaca aagaacatca aaataaaatt actgcattca cggtttaccc taaaggatag
     8221 ggccgacatc gaaaatgaaa tgattaataa agaatttaat aatccaaagc cgccagagga
     8281 aagagaagca aaaatactta tcgcgacaca agttgttgaa gcatcactcg atattgacgc
     8341 tgacgttctt tttaccgaaa tagcgccgat ggactctctt gtgcagcgta tggggcgtgt
     8401 catgcgaaga tataaaactg ccagtgaagt aactgaagat ggaaaagtta atatctttta
     8461 ctcttataaa ctcagggaca aaggttttga atttgaatcc ggtagcggct atgtttacga
     8521 taacgactta ttgattttaa ccattgctga attaaatggg caagacgcgg aagaatatct
     8581 gacattgaag tattctaagg atacgaagaa agttgaaaaa aaatcaaaga agtcggtcaa
     8641 cgacattctc caatctattc ttgaacatga atgcatttca aaaccaatta gtgagcaaaa
     8701 gaaaaaagaa atggttggta atgtgtttaa taaactttca aatgattcac gatacaagaa
     8761 acgcttttac gaaatgctct ctgtattgga tgcaggttat atttcggata ggaaagctga
     8821 tgcagccagg ctatttaggg aaatatttac aatacctgta atagttttta aggatgcaaa
     8881 tgaatttata aaatccgtta cagatatctg cctaagtggt gattgcgaat gggtaagatt
     8941 taaaaaggat gttctatcaa ggtttacaat ccatgtaagt aaatatgcga ttgatatttc
     9001 atcatccgca agcacttccg atcttcagta tgatccttca atcgataagg ataaaatgaa
     9061 gaaaatactt cgctggacag aagatatacg cattataaaa gatggaaaat acgatgataa
     9121 attaggtttt atccctgacc cgaaaaagga ctggagatgg gacatgtaaa tgctgaacac
     9181 cccaatatca ccggcaccca ggtcaactat tatctgatct gtcatcggaa gctctggctg
     9241 ttttcgcaca atatcagtat ggagcatacg tcggaagcgg tggaaatcgg gtcgctggtg
     9301 catgagcaca cttacgagcg caaaaataaa gagctggttt tcgacggcat caagattgat
     9361 ttctttgaca agaatcatgg cattattcat gaggtaaaaa aatcgaaaaa gatggaagag
     9421 gcacatttct ggcagcttaa atattacatt tatcatttca agaagctggg gctttctgtg
     9481 tccggaaaga tcgattatcc tctgattcgc cggacgcaaa cggtcgaact cacagcggaa
     9541 gacgaagcgc aacttgagcg aacgctggac gatattgtca caatcaatga acgggaactg
     9601 ccgccgccgg tcatcaaggc aaagatttgc aagtcgtgca gttactatga actctgttat
     9661 gtgtgaaggc tattaacgga cattgcacat gaacattacg cacgtcatac tatgaactct
     9721 gttatgtgtg aaggctatgc gtgattatta cattttcaaa tccggacgtg tcagtcgcaa
     9781 ggacaattct gtcatgttcg aatatacgga agacgggcaa gaccggcatg tgcccatacc
     9841 gataaacgac attgacgcgc tgtatctttt cggcgaggtg gatttgaaca ccaagatgct
     9901 gaacttcttt gcgcaaagca atgtgatggt tcacttcttc aattactatg gttactacac
     9961 cggcacgttt tatccgcgtg aatttctcaa ttccggcgat gtgatcgtca aacaggttgc
    10021 ctcgtacctt aggaaatcgg tgcgtctgga gctggcgaaa gaattcgtca aaggcgccgc
    10081 ttacggaatc attcagaatc ttaaacgata caaagaaaag acgtcgggag aactggaaaa
    10141 ggtcgaaacc tattttgcgg acatcgacaa gcaatcgacc attgcgcaag tcatgggcct
    10201 ggaaggcaac atcaaggaga cctattacgc ggccttccct aaaatcatta atcaggatat
    10261 cgaatttgaa aaacgcgtga agaatccgcc gaacaatatg atgaacgcgt tgatttcttt
    10321 cgtcaacagc atgatttaca cggaaatgct ccgcgaaatt tacaagacgc aactcaatcc
    10381 gaccatcagt tatctgcacg agccggggta tcggcggttt tccttatcgc tggacatcgc
    10441 cgagatattc aagcccattt atggtgaccg catcattttt gcgttgctta acaataacga
    10501 gctgcaaaag aagcatttcg acaaggatgt caattacgcc tacctgatag aagaagggcg
    10561 caagatcgtc gtcaaggcga ttgacgagaa gttgaacaca acgattatgc acaagcaact
    10621 caaacagcag gtcagttacc ggaagatcat gcggctcgaa ctttacaaac tggtcaagca
    10681 tgtcctgggc gagagcaagt atcagagctt tagaatatgg tggtgatgca tgtacgtgat
    10741 tgcggtttat gacatatcga caatagaacc cgcgggtcaa agacggctgg cgcagattat
    10801 gaagaagatg aggcaatacc tgcaccatac gcaaaagtct gtttttgaag gcgagatttc
    10861 agtggctaaa ttgatgtcac tgcaaaaaga agcaaaaaat atcatcaacg aagaaaccga
    10921 ttatgtggtg ttttttcgaa tcggtaacaa gaaaaacgtg gaaagggagt gcatcggcat
    10981 tcaattggac ccgactgatg tgattattta ataattattt taggcctatg cattattgat
    11041 acttcgagaa aatattgcat aaattaagag aaagaagaag aggcaattat tggattttgt
    11101 atattttgtt attttaaaat taatgggtgt tagtaaacaa tgatgggatt taaagcattt
    11161 gctactgaca ctgccctacc ttccataact tgtgttagta aacaatgatg ggatttaaag
    11221 taatttgtgg atggcggttg taatt
//
LOCUS       DAONQZ010000073        11127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_6451_length_11127_cov_16.479227, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000073 DAONQZ010000000
VERSION     DAONQZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6451_length_11127_cov_16.479227"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLR13_09805"
     CDS             <1..514
                     /locus_tag="PLR13_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19675.1"
                     /translation="KIMQSGIRKKFFADVRADTIVKNPDLMKKWKEAGLYAVVIGFED
                     FQDARLDGYNKKYQGRVIHEAIEILHDLGIIMVGDFIASPDYDESDFEKLEAFIVAHK
                     IQIPVISVLTPIPGTPLYEKMKKDIVTDDLDYYTFTNAVTKTKMPEARFYKTFADMVK
                     RLHRQPHKTT"
     gene            527..1669
                     /locus_tag="PLR13_09810"
     CDS             527..1669
                     /locus_tag="PLR13_09810"
                     /inference="COORDINATES: protein motif:HMM:NF005293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQC19676.1"
                     /translation="MDVYINDIAAFLPNDPVTNDEMETVLGAVHNIPSRIKNRILKNN
                     GIEKRYYAIDRKTGELNYTNARLTAEAVRRLKPYEGFSVNDIQMLCCGTSTPDVLLPG
                     HGLMVQGELGIPSCEVITTAGICLCGITSFKTAYANVALGLSDNAVSTGSELASSLIK
                     SNFFEHLHGDRDFKNHPVLPFESDFLRWMLSDAAGAVFMSGKKNRNGLSLRVDWIENI
                     AYAGQMKTCMYAGAHKKEDGTVIGWRQLHSLEEALSSNYFALKQDTELLNREIVRVMI
                     DLGLSHTVERRKIKPDDIDWYLPHYSSDFFREKIFNAMEGIGFRIPFEKWFTNLAYKG
                     NTGSASIYVIMEELFQSGQLKSGQKLLCFIPESGRFSVAYVLLTVV"
     gene            complement(1678..1968)
                     /locus_tag="PLR13_09815"
     CDS             complement(1678..1968)
                     /locus_tag="PLR13_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HQC19677.1"
                     /translation="MEQEFKTVSVMIDLYCRGHHDSRDHRCVDCEALLTYVGQRLEKC
                     PLKTNKPKCSKCPVHCYKPDMREKIKAVMRYAGPRMIYRHPILAGKHFLNKN"
     gene            complement(1975..2712)
                     /locus_tag="PLR13_09820"
     CDS             complement(1975..2712)
                     /locus_tag="PLR13_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HQC19678.1"
                     /translation="MKILRKIIEIEEELCNGCGNCVTGCAEGALQIIDGKAKVVSETF
                     CDGLGACIGECPTGALKIVEREASPFDEEAVKKHLAKKEKEPETMPCGCPSTHIQIFD
                     KPSACAEANKPKHFDNVEQESVLTHWPIQIKLIPASAPFLKGGDLLILADCTAVAFPT
                     LHRDLMKGKVVMMGCPKFDDVPEYVQKFADIFKTAGIRSVTVVVMEVPCCSGMPMIVK
                     KGMEAAGKEIPMKEIVLSLRGKILKSS"
     gene            complement(2716..4239)
                     /locus_tag="PLR13_09825"
     CDS             complement(2716..4239)
                     /locus_tag="PLR13_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQC19679.1"
                     /translation="MSSERNEQNIVIVSRGNEVIETITLGGHMIIGRAAGADIQLEDT
                     ALSRIHAMFQKDAGRWFLVDLNSLNGTLLNGRKIAPMQPTAVNSGDIAELGEYILRFQ
                     IESQPVAPDQTRPRFVTPDIEPPPSASPLLENLVERHHEIPLWTQGDIQLKIADIIDE
                     TDDVKTFRLVGLSPVLFSFKPGQFVTIKVNINGTDVRRSYTISSSPSRPHCLEMTIKR
                     TPQGLVSNWMHDHVKLGDHLAVHGPSGLFSCFNYPSQKILFIGAGSGITPVMSMLRWI
                     VDTAADVDARLLVSAKSPREIIFKNELDWMSSRHSGIRVGITCTSRVTGADAWTGLTG
                     RCDEKMLKLFTPDILERHIFMCGPSPFMEAVKENLKLLGFNLAHLHTESFGEGRVAKG
                     EKVDARDVPKPPISAVQPVESAPAETPKAASFQVNFIKSGKSISTAGDATLLELAEAN
                     GVEIEYACRSGVCGTCKSKLNKGKVEMSENELSDEEKADGYIYPCVAHPVSDVEIEA"
     gene            complement(4340..5977)
                     /gene="hcp"
                     /locus_tag="PLR13_09830"
                     /gene_synonym="priS"
     CDS             complement(4340..5977)
                     /gene="hcp"
                     /locus_tag="PLR13_09830"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQC19680.1"
                     /translation="MFCYQCQETVKNQGCTVKGVCGKPDATAALQDILIYVCKGIAVA
                     AEKLNAVDKKASRYVCEALFTTITNVAWDDGRIIDKIEEGLKIRDEVNAKAASSLSGA
                     QPDCVTWTPKSREEIMAKAMSEEVRINAASNEDVTSLRSLLIFGLKGIAAYAEHAAVL
                     GYEKDDVYAFIMEALASTTKDLSVEAMIAMVMKAGESAVNTMALLDEANTKTYGNPEI
                     TEVNIGVRNNPGILISGHDLKDMEELLRQTEGAGVDVYTHGEMLPANYYPAFKKYSHF
                     VGNYGSSWWHQNEEFETFNGPIILTTNCLIPIKKDNTYLNRLFTTSVVNYPGAKHIAD
                     RPAGGAKDFSDVIAMAKTCPPPKEIETGKIVGGFAHNQVLALADKVVTAVKSGAIKRF
                     VVMAGCDGRQKARGYFTEVAEALPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLDAGQ
                     CNDSYSLAVIALKLKEVFGLKDINELPISFDIAWYEQKAVAVLLALLYLNVKGIRLGP
                     TLPAFLSPTVAKVLVEKFDIKPIGTVADDVAAMMAGK"
     gene            6098..6787
                     /locus_tag="PLR13_09835"
     CDS             6098..6787
                     /locus_tag="PLR13_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQC19681.1"
                     /translation="MKKARDILIRSQLFGGLPQEYITEIEKIAVDKHYNKGDIIFYDG
                     DNGVGFYLVAAGSVNVYKLSPDGKEQIFHIVKEGETIGAVPVFSGKSFPANARALSKT
                     HLLFFDRIKFIELISGKPDLTMNILALLSMRLREFTVQVENLSLKEIPGRLASYLLYL
                     AQEQGNKHFVKLNISKLQLSNILGTVPESLSRALSNMKAKKLIEEEDAGIRLLNRAEL
                     EDLAERGKTSN"
     gene            complement(7029..7469)
                     /locus_tag="PLR13_09840"
     CDS             complement(7029..7469)
                     /locus_tag="PLR13_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19682.1"
                     /translation="MKKIDKTLLDIRSFEGEGYQPLIDCDQWRVAILRYCDELLPERI
                     FAVQRHNETDEVFVLLEGRCILFLGEGKDTVTSITAVDMQPRKIYNVKRGVWHSHTLD
                     RDTSVLIVENRDTTVTNSPKIILNDDHRRKIVDITRELWLEENQ"
     gene            complement(7666..9225)
                     /locus_tag="PLR13_09845"
     CDS             complement(7666..9225)
                     /locus_tag="PLR13_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19683.1"
                     /translation="MKKYVLVIVVLFLSFLHVGMLYAEKKADFGGAGRYAAVGFAIDG
                     KGYVGTGYDGSTAYKDFWEYDPVANVWTQKADFGGGKRYAAAGFVIDGKGYVGTGYDD
                     SINYKDFWAYDPVANVWTRKTDFGGVIRNGAVGFAVGDIGYIGTGGDGSTNYKDFWAY
                     DPMADAWTQKTEFGGEERIFAVGFSIGSKGYVGTGLNGATKFKDFWVYDPATDAWVQK
                     INAGETARYGAVGFPMDDKGYVGMGGDGATAYKDFGEYNPATDTWTPKVDFEGAARGL
                     AVGFSIGSKGYIGLGLSETAKHKDFWQYDPNLDKTPDPFTFVDQTEVDWNRTITSNTI
                     TVSGVDASVVISITGGTYSINGGDYTSEDGTVNNGDTVTVRQTSSSVFHTTTDAELTI
                     GDISDTFSVTTKYLYDAGDNGPCFIATAAFGSPMAGQVEILRWFRDNYLLTNDAGRKF
                     VAWYYRYGSLAANFIKDKPLIRATVRWALYPLIGFALLLMSGTWPFVMIGILLSTFIC
                     LRVRSGKSKPV"
     gene            complement(9274..9630)
                     /locus_tag="PLR13_09850"
     CDS             complement(9274..9630)
                     /locus_tag="PLR13_09850"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HQC19684.1"
                     /translation="MMVISALVVVFQTELSVCKEAVNKETGQETKVRRGPYADAKITF
                     RIIPSASNTYGYDILMNGKTFIHQPNIPALPGNKGFATPDKAAKVASLVAAKIRNNDM
                     PPVVTMDDLNRMGVLK"
     gene            9867..10211
                     /locus_tag="PLR13_09855"
     CDS             9867..10211
                     /locus_tag="PLR13_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19685.1"
                     /translation="MPDLLIKVALYQSNYLAAGAASGAGAASGAGAGAASGAGAGAAS
                     GAGASTAGASAGAVSSAFLQPTTANDSEATNSIATKIANIFFINREPPFKDFRDLVYG
                     PSFCMGDTLYSI"
     gene            10660..10736
                     /locus_tag="PLR13_09860"
     tRNA            10660..10736
                     /locus_tag="PLR13_09860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10694..10696,aa:Arg,seq:cct)
BASE COUNT         2781 a         2897 c         2558 g         2891 t
ORIGIN      
        1 aaaaatcatg caatccggta tccggaaaaa gttttttgcc gatgtccggg ccgacacaat
       61 cgtcaaaaat ccggatctga tgaaaaaatg gaaagaggcc ggactttacg cggtggtgat
      121 cggttttgag gattttcagg acgctcgcct tgacggctat aacaaaaaat atcagggacg
      181 cgtcatccat gaggccattg aaattctgca tgacctgggc atcatcatgg tcggcgattt
      241 catcgcctcg cccgattatg acgaaagcga tttcgaaaaa ctggaggcgt ttattgtcgc
      301 ccataaaatc cagattccgg tgatttcggt tttaacgccc attccgggca cgccgcttta
      361 tgaaaagatg aaaaaagata ttgtcactga cgatctggat tactatacgt ttacgaacgc
      421 cgtaacgaaa acaaaaatgc cggaagcccg gttctataaa acatttgccg atatggtcaa
      481 aagactgcat cgtcagccgc acaaaaccac ttaaagaaag gactttatgg acgtttacat
      541 caacgacatt gccgcgtttc tgcccaacga tccggtgact aacgacgaaa tggaaacggt
      601 gctgggcgcc gtgcacaata ttccctcccg gataaaaaac agaattttga aaaataacgg
      661 catcgaaaaa cgctattacg ccattgaccg caaaacgggc gaactcaact acaccaatgc
      721 ccggctgacc gccgaagccg tacgcagatt gaaaccctat gaaggctttt cggtcaacga
      781 catccagatg ctctgttgcg gaacctccac tcccgacgtg cttctccccg gtcacggtct
      841 gatggtgcag ggcgaactgg gcatcccgtc gtgcgaagtc atcacaaccg cgggcatctg
      901 cctgtgcggc atcacgtcat tcaagaccgc ttatgccaac gtcgctctgg ggctgagcga
      961 caacgccgtg tctaccggct cggaactggc atcatccttg atcaagtcga atttttttga
     1021 gcatctccat ggcgatcgcg atttcaagaa tcatcccgtc ctgccctttg agtcggattt
     1081 cctgcgctgg atgctctccg acgcggccgg cgccgttttt atgtccggca aaaaaaaccg
     1141 aaacggtctt tcgttgcgcg tggactggat cgaaaacatt gcttatgcgg gacagatgaa
     1201 aacctgcatg tacgccggcg cgcacaaaaa agaagacggc acagtgatcg gctggcgtca
     1261 gcttcattcg ctggaagagg cgctgagcag taattatttc gctctcaagc aggacaccga
     1321 actgctcaac cgggaaatcg tccgcgtgat gatcgatctg ggcctttccc ataccgttga
     1381 acgaagaaaa ataaaaccgg atgacataga ctggtacctt cctcattatt catcggattt
     1441 cttccgggaa aaaatcttta acgccatgga aggaatcggt ttcagaattc cctttgaaaa
     1501 gtggtttacc aacctggcct acaagggcaa cacggggtct gcttccattt acgtgatcat
     1561 ggaagaattg ttccagtcgg gtcaattgaa gagcggacag aaacttctct gctttatacc
     1621 ggaaagcggc cggttttccg tcgcctatgt tctgctgacg gtggtctgag tcctgtatca
     1681 atttttattc agaaaatgtt ttcccgccag aatgggatgg cgataaatca tgcgcggacc
     1741 ggcataccgc atcaccgctt tgattttttc ccgcatgtcc ggtttatagc agtggacggg
     1801 acattttgag catttgggct tattcgtctt gagtggacat ttttccaggc gttgtccgac
     1861 gtaggtcagc aacgcttcgc agtcaacgca acgatgatcc cgactgtcat gatgaccgcg
     1921 gcagtaaaga tcaatcatca cactgacggt tttaaattcc tgttccattg tccttcatga
     1981 agactttaag atctttcccc tcaagctcag cacgatttct ttcatgggaa tctccttgcc
     2041 tgccgcctcc atgccttttt tgacaatcat cggcattccc gaacagcagg gcacctccat
     2101 caccaccacg gtgacgctcc tgattccggc ggttttgaag atgtcggcaa atttttgcac
     2161 atactccggg acatcgtcaa atttcggaca tcccatcatc accacttttc ccttcattaa
     2221 atcgcgatga agggtgggaa aggcgacggc cgtgcagtcc gcaaggatca gcaggtcgcc
     2281 gcctttcagg aagggggcac tggccggaat taatttgatc tgaatcggcc agtgggtgag
     2341 aacggattct tgttccacat tgtcgaagtg tttcggctta ttggcctctg cgcaggcgga
     2401 gggtttgtca aaaatctgaa tgtgagttga cggacaaccg caaggcatcg tctccggttc
     2461 tttttccttt tttgccagat gttttttgac ggcttcttca tcaaacggag acgcttcgcg
     2521 ctcgacaatt ttcagcgcac ccgtagggca ttcgccgata caggcaccca ggccgtcgca
     2581 gaacgtctcc gataccacct tggccttgcc gtcgatgatt tgcagagcgc cctcggcgca
     2641 gccggtcacg caattgccgc aaccgttaca caattcttct tcaatctcga taatcttgcg
     2701 taatattttc ataatttatg cctctatttc tacatccgac accggatgtg ccacacaggg
     2761 gtagatgtat ccgtccgctt tttcctcatc ggacagttcg ttttcactca tttctacctt
     2821 gcccttgttc agtttagatt tgcatgtccc gcagacgccg gaccggcacg cgtattcaat
     2881 ctccacaccg ttcgcttccg ccagttcaag caacgtcgca tctcccgcgg tcgatatgga
     2941 ttttcccgac ttgatgaagt tcacctgaaa gcttgccgct ttcggcgtct ccgccggtgc
     3001 gctttcaacc ggctgaactg ccgagatagg cggctttgga acgtcgcgcg catcaacctt
     3061 ctcccctttg gccacccgcc cttcgccaaa actttccgtg tgcagatggg caagattaaa
     3121 accgagtaat tttaaatttt ccttaaccgc ttccatgaac ggcgaaggac cgcacatgaa
     3181 aatatgccgc tccagaatat ccggcgtgaa cagtttcagc attttctcgt cacagcggcc
     3241 ggtcagacca gtccatgcat ccgcgccggt gacgcggctg gtgcaggtta tacccacgcg
     3301 gatgccgctg tggcgcgacg acatccagtc aagctcgttt ttgaagatga tctcgcgcgg
     3361 agacttggca gataccagca ggcgcgcgtc cacatcggcc gccgtgtcca cgatccagcg
     3421 gagcatggac atgaccggtg tgatcccgct gcccgcaccg ataaacagaa tcttttgcga
     3481 cgggtagtta aagcacgaaa acaatcccga cggaccgtgc acggcaagat ggtcgccgag
     3541 cttcacatgg tcgtgcatcc agttcgagac caggccctgc ggtgtgcgct tgatcgtcat
     3601 ttccaggcag tgtggacgcg aagggctcga tgagatcgtg taagaacggc gcacgtccgt
     3661 gccgttgatg ttcaccttga tggtgacgaa ctgaccgggt ttgaaggaaa acagaaccgg
     3721 cgacaagccg accagtcgaa atgtcttgac atcatccgtt tcgtcgatga tatcggcgat
     3781 cttcagttga atatctcctt gcgtccacag aggaatttcg tgatgtcttt cgaccaaatt
     3841 ttccaggagc ggactggctg acggaggcgg ttcaatgtcg ggcgtcacaa aacgcggacg
     3901 cgtctggtca ggcgcgaccg gttgagattc aatttgaaat ctcaatatat actcacccaa
     3961 ttcggcaata tctcctgaat taaccgctgt cggctgcata ggggcgatct tccggccgtt
     4021 cagcaacgtg ccgttgaggc tgttgagatc caccagaaac cagcggcccg cgtccttctg
     4081 aaacatggcg tgaatgcgcg acagggcggt atcttcaagc tgaatatccg cacccgcggc
     4141 gcgtccgatg atcatatgac cgccgagggt tatggtttca atcacttcat tgccgcggga
     4201 cacgatcact atattctgtt cgtttctttc tgacgacaca ggggaattcc tctctttttt
     4261 cacgattctc tctttctgct ggtgactctc ccgaattttt catccgggag aatcaccgag
     4321 tttaatgatc ggccatcgat tatttgccgg ccatcatagc ggcaacatca tccgcgactg
     4381 tgccgatcgg tttgatatcg aatttttcca ccagcacctt cgcaaccgtg ggtgacaaga
     4441 aggccggcag cgtcgggccc aggcggattc ccttgacgtt gagatacagc agggccaaca
     4501 gcacggccac cgccttctgc tcgtaccagg cgatatcaaa ggaaatcggc aattcgttga
     4561 tgtctttgag gccgaaaact tccttcagct tcaacgcgat caccgccagc gagtaggagt
     4621 cattgcactg tcctgcatcg agcactctgg gaatgccgcc gatatcgccc agcggcaatt
     4681 tgttgtagcg atacttggcg cagcctgcgg tcagaatcac ggtgtctttg ggcagcgctt
     4741 cggcgacttc ggtaaagtag ccgcgagctt tttgtcttcc atcgcaacca gccataacga
     4801 caaagcgttt gatcgcaccc gatttcacgg cggtgacgac cttgtcggcg agcgccagca
     4861 cctggttatg cgcaaatcca ccgacaatct tgccggtttc aatttccttg ggcggggggc
     4921 aggtcttcgc catagcgatg acatccgaga aatcctttgc gccgcctgcg ggcctatccg
     4981 cgatatgttt ggctccggga tagttgacaa cgctggtcgt aaacagacgg ttcaaatacg
     5041 tattgtcttt ttttatcgga atcaaacaat tggtggtcag aataatcgga ccgttgaagg
     5101 tttcaaactc ctcgttctga tgccaccacg acgagccgta gttgcccacg aaatgactgt
     5161 attttttaaa cgccggataa tagttggccg gcagcatctc gccgtgcgta tacacatcca
     5221 cgccggcacc ttccgtttgc ctgagcaact cttccatatc cttcaggtcg tggcctgaaa
     5281 tcagaatgcc gggattattg cgaacgccaa tgttgacttc cgtaatttcg ggattcccgt
     5341 aggttttggt attggcctca tccagaagcg ccatggtgtt cacagccgac tcaccggctt
     5401 tcatgaccat ggcaatcatt gcctccacgg aaagatcttt tgtggtggat gccagcgctt
     5461 ccatgatgaa ggcatagaca tcatctttct catagcccaa aacggcggca tgttccgcgt
     5521 aggcagcaat gcccttgagt ccgaaaatga gcagggatcg cagggaggtt acgtcctcat
     5581 tcgacgcggc attgatgcgc acttcctccg acatggcctt agccataatc tcttctctac
     5641 ttttcggtgt ccatgtgaca cagtcaggct gtgcgcccga cagggaggaa gccgcctttg
     5701 cgttgacctc gtcacggatc ttcagtcctt cttcaatttt atcaattatg cgaccatcgt
     5761 cccaggctac gttggtaatg gtggtgaaaa gcgcctcgca aacataacga cttgcttttt
     5821 tatccaccgc atttaatttt tcagcggcga ccgcaatacc cttgcacaca taaatgagaa
     5881 tatcctgaag ggccgctgtt gcatcgggct tgccgcaaac acctttgacc gtgcatccct
     5941 gatttttaac cgtttcctga cattgatagc aaaacatatg aaacctccct gtaaaagatt
     6001 atttgtcttt ttgttggtga gtatagttaa aaaactaacg cgtttccttg acttatgtta
     6061 agaaaacgat atattttttc tccgaaggac gtaacctatg aaaaaagcgc gggatattct
     6121 aatcagaagc cagcttttcg gtggattgcc tcaagagtat atcacagaaa tcgaaaaaat
     6181 tgcagtcgat aaacattaca ataaaggcga tattattttt tatgatggag acaacggtgt
     6241 cggattttat ctagttgccg ccggtagcgt caatgtttac aaactctctc ccgacggcaa
     6301 agaacagatt ttccatattg taaaggaggg cgaaaccatc ggcgcggtac ccgttttttc
     6361 cggcaagtct tttcccgcca atgcccgggc gctttccaaa acgcatcttt tatttttcga
     6421 ccggataaaa ttcattgagc tcatttccgg caagccggat ctcaccatga atattctggc
     6481 gctgctttcc atgcgcttgc gtgaattcac cgtccaggtg gaaaatctgt ccctcaagga
     6541 aatacccggc cgcctcgcct cctatctgct ttatcttgcg caggaacagg gcaacaagca
     6601 tttcgtcaaa cttaacattt ccaaattaca actttccaac attctgggga cggtgccgga
     6661 atcactttcc agagccttga gcaatatgaa ggcgaaaaag cttatcgagg aagaagatgc
     6721 cggtatccga ttgctcaacc gagccgagtt ggaagatctg gccgaaagag ggaagacctc
     6781 aaattaatcg aacggcaaga gaagatccat tggttgagaa atccctgcct gcgttgatgc
     6841 gcattgagaa atgaatctga ccacagcagc gcccattctg cgtgatcgtt ttaaaaccgt
     6901 tcagaaaata acgaacctcg cggcgatgaa agcatcgcgg taacggacgg tttgcaaacc
     6961 gcttaaaaat ttaccggcag gttagatcgg actttctcca catcgtcctt ttttcattgc
     7021 tcaaacgatt attgattttc ttcaagccac aactcgcgtg tgatatccac aattttccgg
     7081 cgatggtcat catttagaat gattttcggc gagtttgtaa ctgttgtgtc gcgattttcc
     7141 acaatcagca cggaggtatc cctatccagc gtatgactgt gccagacgcc gcgcttaacg
     7201 ttgtaaattt tccgaggctg catatcgacg gctgtaatcg acgtgaccgt atcttttcct
     7261 tctcccagaa aaaggatgca gcgcccttcc aaaagcacaa acacctcatc ggtttcattg
     7321 tgccgctgca cggcgaaaat gcgttcaggc agcagttcat cacaataacg cagaatcgcc
     7381 acacgccatt gatcgcaatc aatcagcggc tggtagccct caccttcaaa actgcgaata
     7441 tccagcagtg ttttatcaat ctttttcact tacgatctcc attttataat atcggttttt
     7501 ctagcatatc ttccgttttc gtgcagccgt tttttgatca ttccagaaaa tggttctatc
     7561 aaaaatccac gaccggttga caggcagggt tgttgacgga ttaaagaaaa cagctcccca
     7621 actttgaaat gttcaggagc tgtcatgttg atccaaatcg atcttttaaa ccggttttga
     7681 tttgcctgat cgtacccgca gacaaatgaa agtcgacaaa agtatcccga tcatcacaaa
     7741 aggccacgtg ccggacatca gcagaagggc aaatccgatt aaggggtaaa gtgcccaccg
     7801 cactgtcgcc ctgatcagag gtttatcttt gataaaattg gctgccagag aaccgtagcg
     7861 gtaataccag gcaacaaact ttcgtccggc atcattggtc agcagataat tgtctctgaa
     7921 ccagcgaagg atttcaacct ggccggccat gggcgatccg aatgccgctg tcgcaataaa
     7981 acagggcccg ttgtcgcctg catcatacag gtatttcgtg gtcacactga aagtatcgga
     8041 gatgtcaccg atagtcagtt cggcatcggt ggtcgtatga aaaacggaag acgatgtctg
     8101 cctgaccgtc acggtatccc cgttattgac agtcccatct tcactggtgt aatcgccgcc
     8161 gttgatggag tatgtgcctc ctgtaatgga gatgacaacg cttgcatcaa cgccggatac
     8221 ggtaatggtg ttggatgtaa tcgtgcggtt ccagtcaacc tccgtttgat cgacaaacgt
     8281 aaacggatca ggggttttat caaggttggg atcgtactgc caaaagtctt tgtgcttcgc
     8341 cgtttcgctc aatcctaacc cgatgtagcc tttgctgccg atggaaaaac cgacggctaa
     8401 acctcttgcc gctccttcga aatcaacttt tggagtccag gtgtcggtgg ccggattgta
     8461 ctctccaaaa tctttatagg ctgtcgcgcc gtcacctccc ataccgacat accccttatc
     8521 gtccatggga aaaccgaccg caccataccg ggctgtttcg ccggcattta tcttttgcac
     8581 ccaggcatcg gtcgccggat cgtataccca aaaatcttta aattttgtcg caccattcaa
     8641 tcctgtaccg acgtaccctt tgctgccgat ggaaaaaccg acggcaaaaa ttctctcttc
     8701 tcctccaaat tcagtctttt gcgtccaggc atcggccatc ggatcgtatg cccaaaaatc
     8761 cttgtaattt gtcgaaccgt cacctccggt accgatatat ccaatatcgc caacggcaaa
     8821 cccgacggca ccatttctga ttacgccgcc gaaatccgtt ttccgggtcc agacattagc
     8881 aaccggatcg tatgcccaaa aatccttgta gtttattgaa tcgtcatacc ctgtaccgac
     8941 atatcccttg ccgtcgataa caaaccccgc agccgcatac cgctttccac cgccgaaatc
     9001 cgccttctgg gtccagacat tggcaaccgg atcatattcc caaaaatctt tataggccgt
     9061 tgaaccatca tatcccgtgc cgacataccc tttgccgtcg atggcaaaac cgacagctgc
     9121 ataccggcct gctccgccga aatccgcctt cttttccgcg taaagcatac cgacatgaag
     9181 aaaacttaag aataagacca cgatgaccag aacatatttt ttcatacgga atctctcctg
     9241 ctggtatctt cacccatcat ctcttgatgg atgttatttc agtaccccca tcctgtttaa
     9301 atcatccatc gtcacgacgg gcggcatatc gttgtttctg attttagccg ccaccaatga
     9361 agccactttt gcggctttgt ccggggttgc aaatcctttg tttcccggca aagccggtat
     9421 attcggctga tggataaaag ttttgccgtt catcaaaata tcatatccat acgtattact
     9481 ggccgaagga ataatcctga aggttatttt tgcatcagca taagggcctc tccggacttt
     9541 agtttcctga cccgtttctt tgttgacagc ctctttacaa acggacagct ccgtttgaaa
     9601 aacgacaacc agtgcagata taaccatcat ggaaaaaacc caaagcaggg tttttctttt
     9661 tcccatcgga aacgatcctc attcagcaag aactttatga ctgtaatttt ttttgcaagt
     9721 cgatgaaata taggcaatct gtcccggact gtcaaggaaa cattgaccgt cacaaaaatc
     9781 atctgcggca gtcatgatta cgtttgcaga cacgtcacgc cttttaaaaa caggtaaaaa
     9841 taataaatcg gacattcaca gaatgaatgc ccgatttatt gataaaggtt gctttgtatc
     9901 aaagcaatta tttggcagca ggagcggctt caggagccgg agcagcttca ggtgcaggtg
     9961 ccggagcggc ttcaggtgca ggtgccggag cggcttcagg tgcaggtgct tcaactgccg
    10021 gagcctctgc aggtgctgtc tcctcagctt ttttgcaacc aacgactgcc aatgacagtg
    10081 aggcaacaaa cagcatagca acaaaaatag caaatatttt tttcatcaac cgtgaacctc
    10141 ctttcaaaga ttttagggac ctggtttatg gaccaagttt ttgtatggga gatacgctat
    10201 actctattta aaagttttgt caagagtcaa aaataaaaag gctttttcat gaacgatcct
    10261 ccttttttcc ggtttccctg aaaatcatat atggattcgt ttaaacaaac atcctttttt
    10321 aagaaaatac cgtaaaggtc ttgacaagca acaccgtttt atgcgttagt gtgcttaatc
    10381 attaacggct caaaagccat ccagtttaat gcctgctcga gatcgcctct cagcaggcat
    10441 tattttattg ttcggattga tccgttcatc ctgcgctagg ccgctctcct tgagcttccc
    10501 gcacatcatg cattgagaaa ttttttatac cataaaagag tggcaccctg tcattcccgg
    10561 cattatcatt tcctgaccga cgatatttgt acatgcccgg gtaattgaca gaaccggtca
    10621 aatcatgtta aagcgcggaa aattctgcga aacaaggttg cccctgtagc tcagaaggat
    10681 agagcaacgg attcctaatc cgtgtgccgc gcgttcgact cgcgccaggg gcaccaatca
    10741 aatcgttaat atcaaagatg ttttcacgca ggccttcctt atatttttgt cacgtctttt
    10801 agagacttcc caaagcaatg ttttttgaaa atttataaaa aaattataga cttttgcctt
    10861 ttcccggtgt aaaaattgcc ttaaatcagc gactaagaat atgcgctctt tcaaaacttt
    10921 tttcacgttc cacaaaaaag tcatggagaa cgtttttctt tcagtaaaaa gaaacaggtt
    10981 ttttgtactc atctaacgtt aaaacacaac aaaaagagaa acaggagaag acgaagagaa
    11041 aggaggtttt atgcgaatca gaaaaggcag taaagcaaag gtgctgtcat ggatcatgac
    11101 ggtcatgttt gtcttatgcg ccggcac
//
LOCUS       DAONQZ010000074        10966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_6590_length_10966_cov_14.920447, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000074 DAONQZ010000000
VERSION     DAONQZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6590_length_10966_cov_14.920447"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLR13_09865"
     CDS             complement(<1..331)
                     /locus_tag="PLR13_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19686.1"
                     /translation="MSSDFSLEFKERYIHLIHAPDYEISPESNDILWAELAKACRKYK
                     CLKVLTEGSNQKRRITSMDAYQSGAQAAESIPGMTLAICIDDYTPDDITQFFKNVAYN
                     RGARIEFF"
     gene            complement(546..851)
                     /locus_tag="PLR13_09870"
     CDS             complement(546..851)
                     /locus_tag="PLR13_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19687.1"
                     /translation="MKEIITATDDHTVESSLGWRVDILSMDALRYQERDKTITFEIED
                     YSDAIGELEWTIYIPPICKWQDENHPEEIIGQEKLDDIIDRISTAFWKLDMKIRGIA"
     gene            complement(893..2065)
                     /locus_tag="PLR13_09875"
     CDS             complement(893..2065)
                     /locus_tag="PLR13_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HQC19688.1"
                     /translation="MNLKIIKKTCLLLWMFLLFTVAAAMPAGAADMENALMECAKIRN
                     NATRLKCFDKLARKPSIKEESTATTIEKKESVAVTAGDMETSEAAAKQTPLSVMEKYW
                     DMSLDPRKERNIFVLWPYRPCYFLPLAYNFSPNKDAALDFDPDAKAKHNEVKFQLSFK
                     FKLWRDIIRDHTIKNIIEKSTGIRGVDLWVAYTQQSFWQLYNDAFSAPFRDTNYEPEL
                     LLNFRMQREIPYLLGTKLQFVNIGFNHQSNGRSEPLSRSWNRIVANVGLEKNFGTATN
                     DSFELLLKTWYRLPEDDADDDNPDLSRYMGYGELWGTLYWKKQRFALMLRNNLRSDNK
                     GAVQLEWSMPMASINENLAKKISLYVQYFNGYGESLLDYNKNVNRISAGLMLADWY"
     gene            complement(2062..2808)
                     /gene="kdsB"
                     /locus_tag="PLR13_09880"
     CDS             complement(2062..2808)
                     /gene="kdsB"
                     /locus_tag="PLR13_09880"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQC19689.1"
                     /translation="MKREVVCIIPSRYESSRFPGKPLADLCGKPMIQHVYERVAKAKA
                     VPYVAVATDDQRIFDAVKHFGGNVVMTAGTHRSGTDRIAEAVKSLNLPADAIVVNIQG
                     DQPIFEPTQVDEVIQPLLDDPSINMSTLIYKIVLDKEITHPHAVKVVLDHQNFALYFS
                     RATIPYVRDKNLRADYYKHHGIYAYRRDFLDVFTKLPEGKLEKLEALEQLRALEYGYK
                     IKVVVTPYDSVEVDNQQELERVREILLEKK"
     gene            complement(2938..3915)
                     /gene="rfaD"
                     /locus_tag="PLR13_09885"
     CDS             complement(2938..3915)
                     /gene="rfaD"
                     /locus_tag="PLR13_09885"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQC19690.1"
                     /translation="MIIVTGGAGFIGSAFVWKLNQEGIDNIIIVDRLGTSDKWKNLVN
                     LRFVDYIHKDDFLEMVDTDMMPFDVDAIIHMGACSSTTERDADYLWHNNYAYTKILAE
                     WALEHDIRFIYASSAATYGDGAKGFADSHKTIPGLKPINMYGYSKQVFDLWVLRQKLE
                     SRVAGIKFFNVFGPNEYHKGDMSSVIFKAFHQIKETGKVKLFKSYKKEYKDGGQMRDF
                     VYVKDCVNVMWWLLQNRKVNGIFNLGTGKARSWNDLIKAVFTAMKKKTNIEYIAMPEA
                     LRNQYQYFTEAEMSKLKKAGCPFKFSSLEDSVYDYVTKYLQKSDPHLGK"
     gene            complement(4117..4971)
                     /gene="pgeF"
                     /locus_tag="PLR13_09890"
     CDS             complement(4117..4971)
                     /gene="pgeF"
                     /locus_tag="PLR13_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQC19691.1"
                     /translation="MFQLNKKHSIQYYESSFLSGCDFLTHAFCTRLGGLSEDDYKSLN
                     ISFKEGDLESRVLQNWNRLAMAFAIPLENFLTLNQVHGDDIFIIKPYGDYLPSDGALN
                     YDAIVTPRENLAICIKTADCVPVFIVDRAKKIIAAVHAGWKGTALEISAKVVRLFLDK
                     YGSYPHDILAAVGPSIGLCCFEVDEDAADSFRNQKDHEAFLFAGNRPNKWIVDLPEAN
                     RRQMINCGISAANIDLSHLCTSCRQDLFYSHRGSGGITGRQLNFIMVKGEAPCRVMTI
                     GDEYPKIQ"
     gene            complement(4975..5988)
                     /locus_tag="PLR13_09895"
     CDS             complement(4975..5988)
                     /locus_tag="PLR13_09895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQC19692.1"
                     /translation="MVGQAEKIKFTVDKSQGGQRLDIYLAQSEAALSRSQVKYAIEEG
                     DVLVNDKVPKVSQHLKEGDVIVLTKRPPVEAIAVPQEMPLDIVYEDDAVIVINKPAGM
                     VVHPAPGNPDKTLVNALLFHCHDLSGIGGVLRPGIVHRLDKDTSGLIVAAKSDDAHRN
                     LSSQFEKHDVHKKYLALVWGDVKGNSGEIVLPVGRHTNNRKKMSTKSRHGKGALTLWK
                     VRERFGVATLLDVEIKTGRTHQIRVHLSDRGYPVVGDAVYGNSAKINTVKDPLLKAKI
                     KEFNRQALHAAQLSFIHPQKGERVVFTADMPQDLADLCAQLRTSIKSVDSKKDMWKDK
                     LKG"
     gene            complement(6126..6719)
                     /locus_tag="PLR13_09900"
     CDS             complement(6126..6719)
                     /locus_tag="PLR13_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HQC19693.1"
                     /translation="MATVKRIKSDNVFQLKITLNHIKPPIWRRVLVDSDIKLPDLHKI
                     IQTVMGWTNSHLHQFIIGNQYYSLPSDESFYKVVDYRRIKLDSLFNTPKSNFIYEYDF
                     GDGWEHSIVIEKILPRKKNTYYPICIDGKRSCPPEDCGGTFGYENLITIINNPEHEEY
                     DEMMEWLGDYFDPKEFNIDEVNEFLREKNFGCVELFD"
     gene            complement(6891..8000)
                     /locus_tag="PLR13_09905"
     CDS             complement(6891..8000)
                     /locus_tag="PLR13_09905"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC19694.1"
                     /translation="MEKLRNLAVPGKVVVIYGARRTGKTTLLKEFLKDETKDHLLVNG
                     EDITVHSYLSSQSVEKLTSFVGNAKLLIVDEAQKIPNIGINLKLIVDHLSGIRMVVTG
                     SSSFDLARHTGEPLTGRKYTLKLFPLAQMEIGQIERIHQTQAHLENRLIYGSYPEVVL
                     MQDNQGREKYLKEIVSSYLYKDILELDGIRQSVKIIRLLQLLAFQIGKEVSLTELGTS
                     LSMSKNTVERYLDLLEKAFVLHRLTGFSRNLRSEIIKNCRYYFLDNGVRNALINNFNP
                     LSQRNDCGELWENYLIMERLKRQEYLDTPANNYFWRTYTRQEIDFVEERQGRLFGYEM
                     KWGKAKPKPPKLWQSAYPESSYELINRENYLKFIT"
     gene            complement(8339..9472)
                     /locus_tag="PLR13_09910"
     CDS             complement(8339..9472)
                     /locus_tag="PLR13_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19695.1"
                     /translation="MKKIFAKISSMNGVYKAFLVYFLILLLALTYKCFPIFDQSETIN
                     NLSFSHDGKKIVFDRCRNVGCQIQVYDLATGELAAYKSPLTKRWTMGKYSYDGKKITF
                     SIIPIKITGDLNLNKMQIAVMDADGKNFKKVTTGPGAKLYPTFSHNGKKILYARAARI
                     REKGKTPAADYDAWEVDLTTGKQTQLSHFEYFYMGYLSYFPDDERFMYYGELPSIFEG
                     ERLSAGGGGYNPRLIELAKRGMGIHAVVEMKGKDLIPTMYNFGDKVVPEKPLLSKDGS
                     VLIFGKAAEAGTFYLYSSDGNHRLVGSGGSVDSAAISPDGTLLGMISVKLGIHIFSVP
                     DGQRQKDLYVPYAKKKIVNWKESHKGNKYLYKMIPEKPSRILN"
     gene            complement(9609..>10966)
                     /gene="selA"
                     /locus_tag="PLR13_09915"
     CDS             complement(9609..>10966)
                     /gene="selA"
                     /locus_tag="PLR13_09915"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HQC19696.1"
                     /translation="LLLEKQNIYDLAPHEIVKETCRRIVQNLRDKIVNAKKKLPAEFI
                     PDAKTTAKEVEQSIKGLYRYSLRRVVNATGVILHTNLGRAPLCPEALERIMEVGKAYS
                     NLEFDLNKGERGQRYDHVSSLLCALTGAEDALIVNNNAAAVLLVLNTLAQKKEAIVSR
                     GELIEIGGEFRIPEIMQKSATKLREVGTTNRTRQSDYEKAINDKTGLIMKVHTSNYRI
                     VGFTEEASIESLVELGKNRGIPVMDDLGSGCLIDLDRYGLQHEPTIRERLAAGVDVVT
                     FSGDKLLGGPQAGIIVGKKEVLAKIKKNPLNRALRIDKFTLAALEATLMHYLNTSAAP
                     KKLRALKALTEPVADVKKRAEKLIEKLQNENFDQLKFSIREDFAAAGGGSLPTQQIPT
                     VLVAVNNKNMTATRMEEKLRKLEMPIIARVDKDEILFDLRTVAEDEFTFIIEGLKQII
                     T"
BASE COUNT         2668 a         2552 c         2476 g         3270 t
ORIGIN      
        1 tgaagaattc gatgcgggcg cctctgttat aggcaacatt tttaaaaaat tgagtaatgt
       61 cgtcaggagt gtaatcgtca atacagatgg ccagagtcat tcccggaatg gactccgccg
      121 cttgagcgcc ggattgataa gcgtccatgg aagtaattct tcttttctgg ttggagcctt
      181 ccgttaagac tttcaggcat ttatatttgc ggcaggcctt ggctaattca gcccatagga
      241 tgtcattgct ttccggcgaa atttcataat ccggtgcatg aattagatga atgtaccttt
      301 ctttaaattc aagagaaaaa tctgaactca tggagcactt ccctaaaaag aaaattgata
      361 gtatttgtgt ttgatcgatt tatacggatt tgtttcagtg atgatttgac atcaattttc
      421 ctcaaaaaat cctttatgaa aacatacaac aaaaggcgtt tttaagcaat cctgtaattt
      481 cgtcattaat tcataacctt aactatcaga ccacagcaga aaaattcttt tcacagaaga
      541 tgtgatcatg ctattcctct aattttcatg tcgagtttcc agaatgccgt gctgattctg
      601 tcgataatgt cgtccagttt ttcctgacca atgatttctt ccggatggtt ttcatcctgc
      661 catttgcata tcggcgggat gtaaattgtc cactcgagtt ctccaatagc gtccgaatag
      721 tcttcgattt caaatgtgat ggttttgtcc ctctcctgat atcgtaaagc atccatggat
      781 aaaatatcga cccgccagcc cagagagctt tcaactgtat ggtcatccgt ggctgttatg
      841 atttctttca taaagataaa ccggttgtgt tttagacgtt tgcatccaaa aatcagtacc
      901 agtccgcaag catcaatccg gcgctgattc gattgacgtt tttattatag tcgagcaggc
      961 tttctccgta tccgttgaaa tactgcacat agaggctgat ctttttggcc agattttcgt
     1021 tgatagaagc catcggcatg ctccattcca gttgtaccgc gcctttgtta tccgaacgta
     1081 aattattgcg cagcatcaat gcgaaccgct gctttttcca gtagagggtt ccccataact
     1141 cgccgtatcc catgtatctg gaaagatcgg gattgtcatc gtcggcatcg tcttcgggaa
     1201 gccggtacca cgtcttgagc agcagttcga aggaatcatt ggtagccgtt ccaaaatttt
     1261 tttccaggcc gacattggct acaatacggt tccagctccg ggagagcggc tcggagcggc
     1321 cgttggactg atgattaaag ccgatgttca caaactgcag ctttgttccc aaaagatagg
     1381 ggatctcccg ctgcatgcgg aaattcaata gcagttccgg ttcatagttg gtatcgcgaa
     1441 agggagcgga aaaggcatcg ttatataatt gccagaacga ctgctgtgta taggcgaccc
     1501 agagatcaac ccctcggatg ccggtacttt tttcgataat attttttatg gtatgatccc
     1561 tgataatatc tcgccagagt ttaaacttga agctcagttg aaattttact tcattgtgtt
     1621 ttgccttggc gtcgggatcg aaatccagcg cggcatcctt gttgggtgaa aaattatacg
     1681 ccagcggcag aaaatagcag ggccggtacg gccagaggac aaaaatattt ctttccttac
     1741 ggggatcaag gctcatatcc cagtattttt ccataacgga taagggcgtt tgctttgcgg
     1801 cggcttcgct tgtttccatg tctccggcgg tgactgcgac agactctttt ttctcgattg
     1861 ttgtggcggt ggattcttct ttgatggatg gcttacgggc cagtttatca aaacatttca
     1921 gtcgggtggc gttgttcctg attttagcgc attccatcag agcgttttcc atgtcggcgg
     1981 cgccggccgg cattgcggcc gcaaccgtaa acagaagaaa catccataag agcagacacg
     2041 tttttttgat tattttaagg ttcatttttt ctccaggagt atctcgcgta ctctttccag
     2101 ttcctgttga ttgtccactt ccacggaatc atagggcgtg acgactactt tgattttgta
     2161 accgtattcg agcgctcgca actgttcaag cgcttcgagt ttttccagtt tgccttcggg
     2221 aagtttggtg aacacatcga gaaaatcccg gcgataggcg taaatgccgt gatgtttgta
     2281 ataatcggct ctcagatttt tatcccggac atacggaatc gtcgcccgcg agaaatacag
     2341 cgcgaaattc tgatgatcga ggacgacctt aacggcgtgc ggatgggtga tttccttgtc
     2401 cagaacaatt ttataaatca gcgtggacat gttgatggac gggtcgtcca gcaggggctg
     2461 aatcacttcg tccacctgcg tcggttcgaa tatcggctga tcgccctgaa tgttgacgac
     2521 aatcgcgtcg gcgggaagat taagcgactt gaccgcttcg gcgatgcggt cagttccgga
     2581 acggtgcgtt cctgctgtca tcacgacgtt gccgccgaaa tgttttaccg catcgaatat
     2641 ccgctggtcg tcggtggcta ccgccacata gggaacggct ttggctttgg ccacgcgttc
     2701 atagacgtgc tgaatcatcg gcttgccgca taaatcagcc agcggcttgc cgggaaaccg
     2761 gctggattca tagcgcgagg ggatgatgca aacaacttct cttttcataa acaactccgt
     2821 aaaaagcctc attagtcatt ccggcggagg ccggaatcca gtgtataaaa ttgttccgct
     2881 tttgcgaaaa taatgtctag actccggcct tcgccgaagt gacgtaagtt gaataattta
     2941 ttttcccaaa tgcggatcgg atttctgcaa atatttcgtt acataatcat agacggaatc
     3001 ttccaacgag gaaaacttaa acggacaacc cgccttcttc agtttgctca tttccgcttc
     3061 ggtaaaatac tgatactgat tccgcagagc ttcaggcatg gcaatatatt caatatttgt
     3121 tttctttttc atggctgtaa aaacagcttt aattaaatca ttccagcttc tcgcttttcc
     3181 cgttcccagg ttgaaaatgc cgttgacctt cctgttttgg agaagccacc acatgacgtt
     3241 gacgcagtct ttgacataaa cgaaatcgcg catctgcccg ccgtctttgt attctttttt
     3301 atacgatttg aaaagtttga ccttgccggt ttctttgatc tgatgaaagg ccttgaaaat
     3361 gacgctggac atatcgcctt tgtggtattc gttggggccg aagacattga aaaatttgat
     3421 tccagccact ctgctttcga gcttctggcg cagtacccac agatcgaata cctgtttgga
     3481 ataaccgtac atattgatgg gtttgagtcc ggggatggtt ttgtggctgt ctgcaaatcc
     3541 ttttgcgccg tcgccatagg ttgccgcgga actggcatag ataaaacgaa tatcgtgctc
     3601 cagcgcccat tcggccagaa tcttggtata cgcgtaattg ttgtgccata ggtaatcggc
     3661 atcgcgttcg gtcgtggagg aacaggcgcc catatggatg atcgcgtcga cgtcgaaagg
     3721 catcatatcg gtatcgacca tttccagaaa atcatctttg tgaatataat cgacgaagcg
     3781 aagattaacc agatttttcc atttatcgct cgttcccaga cggtcaacga tgataatgtt
     3841 atcaatgcct tcctggttga gtttccagac aaaagcgctg ccgataaatc cggcgccacc
     3901 tgtaacgata atcatttgtt cactccttta tttcatttca aaatcaagcg ctatctttgt
     3961 tggcgtcgtt gtcggcttcc tcaacgtatg tacaatacgt ctcgtcgcct cctcctagcc
     4021 gccgcgatat catcattgat ttagaaacga aatcgttttt tgaagacgat gatcttaatt
     4081 aatactgaaa agaaatactg tcaatagcca tgttttttac tgaatctttg gatattcgtc
     4141 gccgatggtc atgacgcggc agggtgcctc acctttcacc atgatgaaat taagctgccg
     4201 gcctgtgatc ccgccggaac cccggtgcga gtaaaacaaa tcctgacggc aggacgtgca
     4261 caaatgggaa aggtcgatat tggctgcgga aatgccgcag ttgatcatct gtctgcggtt
     4321 ggcttccggt aaatccacga tccatttatt cggtctattg cctgcgaata aaaacgcttc
     4381 gtgatctttt tgattgcgaa aagaatccgc cgcgtcttca tcaacttcaa agcagcagag
     4441 tccgatcgag gggccaacgg ccgccagaat atcgtgcgga tatgatccgt atttgtccag
     4501 gaaaagacga acgactttgg cgctgatttc cagcgcggtg cctttccatc cggcatggac
     4561 tgccgcaatg attttttttg cccggtcaac gatgaacacc ggcacgcaat ccgcggtttt
     4621 gatgcagatc gccaggttct cacggggcgt tacaatagcg tcgtaattca gggctccgtc
     4681 cgaaggtaaa taatcgccgt agggtttgat tataaaaata tcgtcgccgt gcacctgatt
     4741 caaggtcagg aagttttcca ggggtatggc aaaggccatg gccagacgat tccagttctg
     4801 caaaaccctg ctttccagat cgccttcctt gaaactgatg ttcagcgatt tataatcgtc
     4861 ttccgagaga ccgccgagcc tggtgcagaa ggcatgcgtc aaaaaatcgc atccgctcaa
     4921 aaacgaggat tcgtaatact ggatggaatg ctttttgttc agttgaaaca tggattaccc
     4981 ttttaattta tccttccaca tatctttttt tgagtcaact gacttgatgc ttgtccgtaa
     5041 ttgagcgcac aaatccgcca gatcctgcgg catgtccgcg gtaaatacca cccgctcgcc
     5101 tttttgcgga tgaatgaaag aaagttgtgc cgcgtgcagt gcctgccggt taaattcctt
     5161 gatctttgcc ttcagcaggg gatctttaac cgtgttgatt ttggcggagt tgccgtaaac
     5221 ggcatcgccc accaccggat aaccgcggtc ggacaaatgc acgcggatct gatgcgtgcg
     5281 tccggtttta atctccacat ccagaagcgt tgccacgccg aagcgttcgc gcactttcca
     5341 aagagtgagc gcgcctttgc cgtgtctgct ttttgtggac atttttttgc ggttgtttgt
     5401 gtgcctgccg acaggcagaa ctatttctcc actattgcct ttcacgtctc cccagacgag
     5461 cgccagatat tttttgtgca catcgtgttt ttcaaactgc gaagaaagat tgcggtgcgc
     5521 gtcatcggat tttgccgcca caataaggcc ggacgtgtcc ttatccagcc ggtgcacaat
     5581 gcccggacgc aagacaccgc cgatgccgga taaatcatgg cagtggaaaa gcaaagcatt
     5641 gacgagcgtt ttatcgggat tcccgggcgc cggatgcacc accatacccg ccggtttgtt
     5701 gatgacgata acggcgtcgt cttcgtaaac gatgtccagc ggcatttcct gcgggacggc
     5761 tatggcctcc accggcggcc ttttggtcag cacaatcacg tcgccttcct tgaggtgctg
     5821 gctgactttg ggaactttat cgttgaccag cacatcgcct tcctcaatgg cgtacttcac
     5881 ctgcgaacgc gaaagcgccg cctcgctctg cgcgagatat atatccagcc tctgcccgcc
     5941 ctggctttta tcaactgtaa attttatttt ttccgcctgt ccgaccatct tattgcctca
     6001 attttgtcat tcccgctaag gcgggaatcc agtctttttc attttgaaaa acttgataac
     6061 tcaaaggcgt tccatgtttc cataaaaata gaaacgaata tattataaat tatcaatacc
     6121 ttcttttagt caaataattc gacacaacca aaattttttt cacggaggaa ttcattcact
     6181 tcatctatat tgaactcctt cgggtcaaaa taatctccaa gccattccat catttcatca
     6241 tattcttcat gttcgggatt gtttattatc gtaataagat tttcataacc aaaggtgcca
     6301 ccacaatctt caggtgggca acttcgtttg ccgtcaatgc atattggata ataagtgttt
     6361 tttttacgag gcagtatttt ttcaataacg attgaatgtt cccaaccatc cccaaaatca
     6421 tattcataaa taaaatttga ttttggcgta ttgaataatg aatccaattt aattcggcga
     6481 taatcaacta ctttgtaaaa tgattcatcg cttggaagtg agtaatattg attgccaata
     6541 ataaattgat gtaaatgaga attagtccag cccattacag tctgtataat tttatgtaaa
     6601 tcaggcagct taatatctga atcaaccaat actcttctcc aaatcggtgg cttaatatga
     6661 ttaagagtta tttttaattg aaatacatta tcagatttaa ttcgtttaac agttgccatg
     6721 tcaacacctt gttcttttag attaaatatt gaaatgtttg tcccactgaa aaatcagcct
     6781 ctgcccgctc tggcttttat ccactataaa actgattttt tcagacgtcc ctaacaagta
     6841 tttgctccgg tattttagtt atggaactgt tcgggaaagc agttcctgca tcaagtaata
     6901 aacttgagat aattctctct gttaatcagc tcataggaag attccggata ggccgactgc
     6961 cagagtttcg gtggcttggg ttttgccttg ccccatttca tttcatagcc gaacaggcgg
     7021 ccttgacgct cttcgacaaa atcaatttcc tgtctggtat aagtccgcca gaaataatta
     7081 ttagccgggg tgtccagata ttcctgcctt ttcaatcttt ccatgatcag gtaattctcc
     7141 cacaattcgc cgcaatcgtt gcgttgcgag agcggattga aattgttgat cagcgcgttg
     7201 cgcacgccgt tgtccagaaa atagtagcgg cagtttttta tgatttcact gcgcaggttg
     7261 cggctgaaac cggtcaagcg atgcaggacg aatgcttttt ccaggaggtc cagatagcgc
     7321 tccacggtat tcttgctcat ggataatgat gttcccaatt cggtcagtga tacttccttg
     7381 cctatctgga aagcaagaag ctggagcagg cggataatct tgacggattg tctgatgccg
     7441 tcgagctcca gaatatcttt atacaaataa gaagacacga tttctttgag atacttttcc
     7501 cgcccttgat tatcctgcat taagacgact tcgggataag aaccgtaaat caggcggttt
     7561 tccagatggg cttgagtttg atgtattctt tcaatctgac cgatttccat ttgcgccagc
     7621 ggaaacaatt tgagcgtgta ttttcgtccg gtcagcggtt cgcccgtgtg acgcgccaga
     7681 tcgaaggacg acgagccggt gacgaccatg cggataccgg atagatgatc aacaatcagc
     7741 ttgagattta ttccgatatt gggaattttc tgcgcttcgt caacaatcag aagttttgcg
     7801 ttgccgacaa aagatgtgag cttttccacc gactggctgg ataaatagga atggacggta
     7861 atatcttctc cgttgaccag cagatgatct tttgtttcgt ctttcagaaa ttctttcagc
     7921 agtgtcgttt tacccgtgcg cctggcgccg taaataacaa ccaccttgcc cggaacggcc
     7981 agatttctta acttttccaa ttgtttttgt gcaatgtaca tataattacc ttggtcattg
     8041 tgttgaccgt attatatata attcagtcag tttgtcaacg gtttttataa tgcctttccg
     8101 gtagataaaa agccttgctt tagcgggcgg gagactgtcc aaggagtcgg aaaaaagggt
     8161 gctgacatat ttctttggga acattgagtc ttgttgagtg cggtatcagt tatcctttat
     8221 ttatctattt tacaatttta agggcgtccc tctttccgac caacttattg ctccggtttt
     8281 accttattat gtcttaccgg cgaagaccgg tatccataca cctttttgac ttttcttatc
     8341 aattgagaat gcgtgaaggt ttttcaggta tcatcttgta taaatattta tttcctttat
     8401 gtgattcttt ccaatttaca atttttttct ttgcatatgg tacatataga tctttctgac
     8461 gttggccgtc agggactgaa aagatatgta tgcctagttt aactgatatc attcccagca
     8521 acgtcccatc tggtgagata gctgcggaat caaccgaacc accactaccc acaagccggt
     8581 gattgccatc cgatgaatag aggtaaaatg ttcctgcctc agcggctttt ccaaatatca
     8641 gtacggatcc gtctttgctc agaagtggct tttcgggaac aaccttgtca ccaaaattat
     8701 acatcgttgg aataagatcc ttacctttca tctcgacaac ggcatgtata cccatacctc
     8761 ttttcgccaa ttcaatcagc ctaggattat agcctcctcc accagcagat aatctctcac
     8821 cctcaaatat tgatggcagt tccccgtaat acatgaatct ttcatcatca ggaaaatatg
     8881 agaggtaacc catgtaaaaa tactcgaaat gagagagttg tgtctgcttg ccggttgtca
     8941 aatcaacttc ccaggcatcg taatccgcag cgggtgtttt gcctttttct ctgatacgag
     9001 ccgcgcgggc atagagtatc ttcttcccgt tatgagaaaa agttggatat aacttggcgc
     9061 cggggccggt ggtaaccttt ttaaagttct tcccgtcagc atccatgacg gctatctgca
     9121 tcttattcaa attgagatca ccggtaattt ttatggggat aatggagaaa gtgattttct
     9181 tcccgtcata agagtacttc cccatagtcc atctttttgt caggggcgat ttataagcag
     9241 ccaattcacc tgttgccaaa tcatagacct gtatctgaca gcctacatta cgacagcggt
     9301 caaacacgat tttctttccg tcatgggaaa aactcaagtt gttgatggtc tccgattgat
     9361 cgaatatggg aaaacattta tatgtcaatg ccaaaaggag tattaaaaag tacaccagaa
     9421 aggccttata cacgccattc atgctggaaa tctttgcaaa gatttttttc atgccgcccc
     9481 cccccctgaa atttatttta cagatacaac gcatgactta agttcagtcg agtcttttgg
     9541 actcgactgt gtatttgtgt tgtagacagc caagtcaaaa agacttggct gaacagagaa
     9601 tgacaatgtt aggtgattat ctgtttcaac ccttcaatga tgaatgtaaa ttcatcttct
     9661 gccacggtgc gcaggtcgaa gaggatttca tccttatcca cgcgggcgat gatcggcatt
     9721 tcaagcttgc gcagtttttc ttccatcctg gtagccgtca tatttttgtt gttgacggca
     9781 actaaaaccg tgggaatctg ctgcgtgggc agggagccgc cgccggctgc cgcgaaatct
     9841 tcacggatgg agaatttcaa ctgatcgaaa ttttcatttt gcaatttttc tattaatttt
     9901 tcagcgcgtt ttttgacatc ggcaacaggt tcggtcaaag ccttcagggc ccgtaatttc
     9961 ttcggcgccg cggaggtatt caagtaatgc atcaaggtgg cttccagagc ggccagcgtg
    10021 aatttgtcga tgcgcagggc gcggttcagg ggattctttt tgattttcgc gaggacctct
    10081 tttttgccga cgatgattcc ggcttgcggg cctcctaaaa gtttgtcgcc gctgaaggtg
    10141 accacgtcaa cgccggcggc cagtctttca cggatcgtcg gttcgtgttg cagaccgtag
    10201 cggtccagat caatcaggca gccgctgccc agatcgtcca tgaccggaat gcccctgttc
    10261 ttacccagtt caaccagcga ttcaatactt gcttcttcgg tgaatcccac aatgcggtaa
    10321 ttgctggtgt ggactttcat gatcagaccc gttttgtcgt tgatggcttt ttcgtagtcg
    10381 ctttgccgtg tacggttggt cgttcccact tcgcgaagtt ttgttgcgct tttttgcatg
    10441 atttccggga tgcggaattc gccgccgatt tcgatgagct cgcctcgcga gacgatggct
    10501 tcttttttct gggccagcgt attgagcacc agcaaaaccg ccgccgcatt gttattgacg
    10561 attaaagcat cttctgcgcc ggtgagggcg caaagcagcg aactgacatg atcgtagcgt
    10621 tggccgcgct cgcctttgtt taaatcaaat tccagattgg agtaggcctt gcccacttcc
    10681 ataattcttt ccagagcttc ggggcaaagc ggcgcgcgtc ccaggttggt gtgcagaata
    10741 acccccgtgg cattgaccac acggcgcagg ctgtagcggt acaacccttt aattgattgt
    10801 tccacctctt tggcggttgt tttagcatcg ggaatgaatt ctgcaggaag cttcttttta
    10861 gcgttgacga ttttatcacg caggttctgc acaatcctgc ggcaggtttc tttgacgatt
    10921 tcgtgcggcg ccagatcata gatgttttgt ttctccagaa gaagaa
//
LOCUS       DAONQZ010000075        10174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_7225_length_10174_cov_19.413677, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000075 DAONQZ010000000
VERSION     DAONQZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7225_length_10174_cov_19.413677"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1155
                     /locus_tag="PLR13_09920"
     CDS             169..1155
                     /locus_tag="PLR13_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQC19697.1"
                     /translation="MSDETKRSEPVESVDVNEDEIDLLELARTIWKGRKLIIWIVCAF
                     TLATIILSLFMTNIYTARAVIKPSSQSQSGGRLASLASQFGGLASLAGIAMPSSASST
                     EIVALLESNILKKEVIETHQLLPVLFKDQWDENTKSWKKPGVSLNPLAFIAKLRPAPK
                     TVKKEPGVPGTWDGIRVLNGIVKINYDMKEDIITISVDFPDPDMAARIANYFVLTLND
                     HMSMEAKRIATTNREYLEQQLTQTSDPIVQQKIYNLIADKIETTMMAEVKEGFAFKVL
                     DPPMAPDKKSKPKRAQMAVVAFMVSVFIGIFAVLFKEYIRKIKENSAGGHHA"
     gene            1148..4627
                     /locus_tag="PLR13_09925"
     CDS             1148..4627
                     /locus_tag="PLR13_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQC19698.1"
                     /translation="MRSQRFFTFLVLCLFCIAVGLAGAGIGRAQTAGSSSLNLNDQRA
                     IQSSLQKAGINLTPEEIRKGKEAFEKQEGVRTGKMYKMPNAPEEQQMQRPEGYLDSRT
                     AVEAPKEVSVFNRIRKIGEYQDVLTDLKPFGYDFFHGTEVKVLTERQDIPVPLDYMIG
                     PGDEVNILLWGRMSAQLTLVVDRDGKITIPDIGPVQVAGMTFGEMSKHLIAKAEQIVG
                     TNIDISMGSTRTIPVFVLGDVERPGTYTIGALATITDALMLARGPSAIGSMRRVELRR
                     KNRVVTKFDLYDLFLKGDKSKDVVLQAGDVVFVPVTGPLVGITGNVKRPAIYELKDRF
                     DLQHLFELAGGIIPSAYTQQMQVERVVKNEKQIVIDINDKTLAKVRDFVLQDADLIKV
                     FSIMDVDANAVYLRGNVKRPGKYSFAPGMKIRDLINQPEDLQSEAHMDYALIKRENPP
                     NREAVLIPFNLGKLILQNDEAYNYDLAPKDQIFVFHQSLFKDQPFVTLEGEVRGFEPT
                     AQEKALRAGQYAQAAPGPYGQAVPGQFGAPAVPGSVQYGQPASGTYSAPAPAQPGTAA
                     PGQYNVAAPRQPAVSPSGQYGQPTAGSYGSQVSGQPGVALPGQYGQTAPAPYGVQVPG
                     QPGAAFPDQYYPYNENLFEIRQIKDTLRFDYEKDKAREKDEIFYLLMAKIEEIEAEIE
                     KDNRVTPGIINQLQIELEKAGRKDLSLRLGKLDEKLKVAIRIDLVGNMKVKDAILNAG
                     GLTANASMESGEIIRQYPNNEFRTTYFNVARAMEGDPRDNLLLQDKDRIVIHSILEKN
                     PRKNIFVTGDVTKPGTYQFTENMTVRDLIFKAGNVLESAYLDEAEITSVSVVNGRLGD
                     LSHRKVNLKKALEGDAGHNVTLLPNDRLHVKQIADYHNVRFITLSGQMAFPGKYPFRK
                     GERLSDIVERAGGFTPHAYLRGAQFTRVRVKELQQQGLKEMADRMERELLSGGAEQFA
                     TAVSKEEIAAKQGELEQKKKFVDTLRKTRATGRMTVYVADAGKLRNTEYDFELEDGDT
                     LYVPERNSVVNVVGSVMSQSSHLYSENLSHQDYIDASGGYSYYADASNVYILKVDGGA
                     RRLSKNFIGWNSSRSRWEMTAYGAGVKDIEPGDTIVVPEKIERIAWLREIKDITQILM
                     NIAVVAGVTIKLF"
     gene            5304..6041
                     /locus_tag="PLR13_09930"
     CDS             5304..6041
                     /locus_tag="PLR13_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19699.1"
                     /translation="MVDRKFKKLSIVVPVFNEERYLEAVIGKVVAQPLPGGLEKELIL
                     INDASQDNSWNIMQALPEKFPSVTMQLINKTVNEGKGAALRDGFAKITGDIVIVQDAD
                     FEYDPADYPKLLQPILDDKADVVYGSRFIGEPHRVLYFWHQVANNILTTISNMLTNLN
                     LTDMEVCYKVFIREVADRLKLKSPRFGVEPEITAKIARMRLNGRHLRVYETGISYAGR
                     TYEEGKKINWKDAVSAIFQIIRFRFMD"
     gene            6322..9132
                     /gene="ppdK"
                     /locus_tag="PLR13_09935"
     CDS             6322..9132
                     /gene="ppdK"
                     /locus_tag="PLR13_09935"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQC19700.1"
                     /translation="MAKYVYSFSEGYGKSKNLLGGKGVGLAEMAHLKIPIPPGFTITT
                     EVCMEYLRNKGRYPQGLKEQVNKALDKMEKETGMRFGDPKNPLLCSARSGARSSMPGM
                     METVLNIGLTSKTIAGMIEKTNNPRFVLDSYRRLIQMYSDVVMEKAEGIEPEEGMGVR
                     QQLEREMAAMKERRNVKLDVDLTDKDLAQLAEIYKAKVKEVLGKPFPDDAMEQLWGAI
                     GAVFKSWNGKRAVSYRRIEGIPDDWGTAVNVQAMVFGNMGENSATGVAFTRNPATGEN
                     KFYGEWLPNAQGEDVVAGIRTPNPINEETRQSDDVDGHPSLEKAMPKAYTEIKAIRNK
                     LEAHFRDMQDLEFTIQDGRVWMLQTRTGKRNGPAAVRMAVEMYKSKMISAEEAISRVT
                     PSQLDEMLHPMVDPKAEATAEFLVKGLPAGPGGAVGQVAFTSDVAMEWRKEGKKVILV
                     REETNPEDVEGMRAAEGVLTARGGMTSHAALVARGWGKCCIVGAGTLHVDIAQKIMKV
                     DGKAVREGDFVTLNGTKGRAYIGSLPMISGAENDVNFAAFMKLCDSLRRLKIRTNAET
                     PKDAAKAVQFGAEGIGLFRTEHMFYGENSEQPLFYLRKMIIADTEEKRRAALDELFPF
                     VKGFIKKTIEAMGGRPVTIRLLDPPLHEFVPENPEERARLAESLGISVEKLNGRAEGL
                     HETNPMMGHRGVRLGISYPEVSEMQIRAILEGSAELIKEGKKALPEIMIPVVCDVAEF
                     SNQAALVKKVYPEVLAKYGLKKMSYMVGTMIEIPRAALLADKIAEEAEFFSFGTNDLT
                     QMTYGFSRDDVGAFVPGYLAKKIIPVDPFQILDQTGVGQLIQMGVERGRSARPGLKVG
                     ICGEHGGEPSSVMFCHRVGMDYVSCSPYRIPLARLAAAQAAVEEKMSSKPKTKAKAKA
                     KTKAKTTVKAKAKVKKAKKSRK"
     gene            9198..>10174
                     /locus_tag="PLR13_09940"
     CDS             9198..>10174
                     /locus_tag="PLR13_09940"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HQC19701.1"
                     /translation="MELAVKNKKLLSWVKEMKQLCAPKKVYWCDGSKKEYNTLMAQMV
                     KSGAAIKLKKRKNCFLFRSDPSDVARVENRTYISYPDQVDAGPTNNWVAPDALKKTMK
                     NLYKGCMKGRTMYIIPFSMGPINSPIAKIGIEITDSPYVVCNMHTMTRVGKKVLKKLG
                     DTGEFIPCMHSIGAPLEKGQKDVAWPCAPIEKKYISHFPDENLIWSYGSGYGGNALLG
                     KKCLALRIASAMAKREGWMAEHMLILRLTNPKGKQYHIAAAFPSACGKTNLAMLQPTI
                     KGWTCECIGDDIAWMKIGPDGRLYAINPEAGFFGVAPGTSYDSNPVAMDT"
BASE COUNT         2671 a         2440 c         2818 g         2245 t
ORIGIN      
        1 gaatttcagt tgcttacggc atgacttcat gttatgcaaa gaagcctttg cgtaattgat
       61 ttttatgact ttttgagcaa caatttcgcc gatccaaaat ttgccgggat tggcaggggc
      121 gagaaatctt tctttttaaa ataaccggaa tattcaggaa aggtccatat gagcgacgaa
      181 acaaaaagat cagaacccgt cgagagcgtt gatgtaaatg aagatgaaat cgatcttctg
      241 gaactggccc gaacaatctg gaaggggcga aagttgatca tctggattgt ctgcgccttc
      301 accctggcga cgatcatttt atctcttttt atgacaaata tttacacggc ccgggcggtg
      361 atcaagccgt catcccagtc gcaaagcggc gggagacttg cctcgctggc cagccagttc
      421 ggcggcctgg ccagcctggc cggaattgcc atgccgtctt cggcatcctc tacggaaatt
      481 gtcgctcttc tggagtccaa tattttgaaa aaagaggtga ttgaaaccca tcaactgctg
      541 ccggtattgt ttaaagacca gtgggatgaa aatacgaaat catggaaaaa gccgggtgtc
      601 agtcttaacc ctctggcctt cattgccaaa ttaagaccgg cgccaaaaac cgttaaaaaa
      661 gaaccgggag ttcccggtac atgggacggc atccgtgttt tgaatggtat cgtcaaaatc
      721 aattatgata tgaaggaaga catcattacc atctccgtcg attttcctga tccggacatg
      781 gccgcccgca tcgccaacta ttttgttctt acattgaatg accatatgag catggaagca
      841 aagaggattg ccacgacgaa ccgggaatat ctggagcagc aactgaccca aaccagcgat
      901 cccattgttc aacaaaaaat ttacaacctc atcgccgata aaattgaaac cacgatgatg
      961 gcggaagtca aagaagggtt tgcgttcaag gtgttggatc cgccgatggc gcccgacaaa
     1021 aagagcaaac ccaaacgggc gcagatggcc gttgtcgctt ttatggtgtc ggtgtttatc
     1081 ggtatttttg cggtattgtt caaagaatac ataagaaaaa tcaaagaaaa ttctgccgga
     1141 gggcatcatg cgtagtcaac gattttttac attccttgtg ctgtgtttat tctgtatcgc
     1201 agtcggtctt gcgggggcgg gcattggccg ggcacagacg gccggttcat cgtctctgaa
     1261 tctcaatgat cagcgggcca ttcaatccag tctgcagaaa gccggcatca atctgacacc
     1321 ggaagagatc cgcaagggca aggaagcctt tgaaaaacag gaaggtgtcc ggacgggcaa
     1381 aatgtataaa atgcccaacg cgcctgaaga gcagcagatg cagcggccgg aaggctacct
     1441 ggacagccgg acggcggttg aagcgcccaa agaagtatcg gtattcaacc ggataagaaa
     1501 gatcggcgaa tatcaggatg tgttaaccga tctgaagcct ttcggttacg atttttttca
     1561 cggtacggaa gtcaaggtgt taacggaacg ccaggatatt ccggttcctc tggattatat
     1621 gatcgggccg ggcgatgaag tgaatatcct tttatggggg cggatgagcg cccaactcac
     1681 gctcgtggtg gaccgggacg gcaaaatcac gattcccgat atcggtcccg tgcaggtggc
     1741 gggcatgacc ttcggtgaaa tgtccaaaca cctcattgcg aaagccgaac agattgtagg
     1801 aacgaatatt gatatttcca tggggtccac ccggacgatt cccgtttttg ttctgggcga
     1861 tgttgaacgt cccggcacgt atacgatcgg cgcattggca accattaccg atgcgctcat
     1921 gctggcgcgc ggtccttcgg ccatcggctc gatgagacgc gtggaactgc gcagaaaaaa
     1981 cagggtcgtg accaaattcg acctgtatga tcttttcctg aaaggcgaca agtccaaaga
     2041 tgtcgtcctg caggcgggcg atgtggtttt tgtacccgtc accggtccgc tggtcggcat
     2101 taccggcaac gtcaaacggc cggcgattta tgaactcaaa gacagattcg acttgcagca
     2161 tctttttgaa ctggccggcg gcatcattcc cagcgcctac acgcagcaga tgcaggtgga
     2221 acgggtcgtt aaaaatgaaa aacagattgt catcgatatc aacgataaga cgctcgccaa
     2281 ggtgagggat ttcgtgcttc aggatgcgga tttgatcaag gtgttttcca ttatggatgt
     2341 ggacgccaac gccgtttatc tgaggggcaa tgtcaaacgt ccggggaaat attcctttgc
     2401 gcccgggatg aagatccggg atctgatcaa ccagccggag gatctgcaga gcgaagctca
     2461 catggattac gcgctgatca aacgggaaaa cccgcccaac cgggaagctg ttctgattcc
     2521 gtttaatctg ggcaaactca ttttgcaaaa tgacgaagcg tacaattatg acctggcgcc
     2581 caaagatcag atttttgtct ttcaccagtc gttattcaag gatcagcctt tcgtcacgct
     2641 ggaaggagaa gttcgcgggt tcgaacctac ggctcaggaa aaagctctga gagcgggcca
     2701 atatgcacag gccgcacccg gtccctacgg ccaggccgtt ccgggccagt tcggcgctcc
     2761 ggccgttccc ggttccgtcc agtacggtca acctgcttcc ggtacctaca gcgctccggc
     2821 gcccgcgcag ccgggtactg cggcgcccgg tcagtacaat gtcgcagcgc cccgtcagcc
     2881 tgcagtttcg ccttcgggcc aatatggaca gccgacggcc ggttcgtacg gctcccaggt
     2941 ttccggtcag cccggggttg cattgcccgg tcaatacggc cagactgccc ccgctccgta
     3001 tggtgttcag gttcccggtc agcccggggc tgcattcccc gatcagtatt acccgtataa
     3061 cgaaaatctg ttcgagatca ggcaaattaa agacacattg cgtttcgatt acgaaaagga
     3121 caaagctagg gaaaaggatg aaattttcta tcttttaatg gccaaaatag aagaaattga
     3181 agcggagatc gaaaaagata acagggtaac gccgggtatc attaatcaac tgcaaattga
     3241 actggagaaa gcgggcagaa aagatctgtc gttgaggctc ggcaaactgg atgagaagtt
     3301 gaaagtggca atccggattg atctggtcgg caacatgaag gtgaaagacg ccattttgaa
     3361 tgccggcggc ctgaccgcaa atgccagcat ggaaagcggc gaaattatcc gccagtatcc
     3421 gaacaatgaa ttcagaacaa cgtatttcaa cgttgccaga gcgatggaag gcgatccgag
     3481 agataatctt ctgctgcagg ataaagacag gatcgtcatt cattcgatcc tggaaaagaa
     3541 tccaaggaaa aatatttttg tcacgggcga tgtcaccaaa ccgggaacgt atcagtttac
     3601 ggaaaacatg actgtgcgcg atctgatttt caaggcaggc aatgttctgg agtccgcgta
     3661 tcttgatgaa gcggaaatta catcggtcag cgttgtgaac ggaagactgg gcgatctttc
     3721 ccacaggaaa gtcaatctga aaaaagcgct ggaaggcgac gccgggcaca atgtgacgct
     3781 gctgcccaac gaccggctgc atgtcaaaca gattgccgat tatcacaatg tgcggtttat
     3841 cacgttatcc ggccagatgg ctttccccgg caagtatccg ttcagaaaag gcgaaagatt
     3901 atccgatatc gttgaacggg ccggcggttt tacaccgcat gcgtatttgc gcggcgctca
     3961 gttcacgcgg gtgcgggtga aagagcttca gcagcagggt ctgaaagaaa tggccgaccg
     4021 catggagcgt gaattattat ccggcggggc ggaacagttt gccaccgctg tttcaaaaga
     4081 ggaaattgcc gccaaacagg gcgagttgga gcaaaagaaa aaatttgtcg acacgctgcg
     4141 caagaccagg gccaccggcc gcatgacggt ttatgtggcg gatgccggaa aactccgaaa
     4201 cacggagtat gattttgagc tggaagacgg cgacacgctg tatgttccgg aaagaaacag
     4261 cgtggtgaat gtggtcggct ccgtgatgtc gcagagcagc catctgtatt ccgaaaatct
     4321 cagccatcag gattacatcg atgcgtccgg cggatactcc tattatgcgg atgcgtccaa
     4381 tgtctacatc ctcaaagtag acggcggcgc ccgacggctt tccaagaatt tcatcggttg
     4441 gaattcttcc cgctcgcgct gggaaatgac ggcgtacggc gccggcgtca aggatatcga
     4501 accgggcgat accattgttg ttccggaaaa gattgaaagg attgcctggc tgcgggaaat
     4561 caaggatatt acgcagatcc tgatgaatat tgccgtagtc gccggtgtca cgatcaaact
     4621 gttctaatga caccgagttg ggacgttaaa attagggttg tcacttcgag cgaatgcgag
     4681 aagtcttgaa aaaggtttga gactttgcaa aaccacgtca attcgtcaat tttgtcatac
     4741 cggcgcaggc cggtatccag aaaatggatc cccgatagga ccactcgggg atgacagagg
     4801 atggatctaa ccagagggcg ccatggcctg tctccacggg aatggcccaa agaaaaagtt
     4861 atgcaaagct ctcagtttgt aactgtgaaa tgaaagcatt aagattcctc ttcactggcg
     4921 ctcatcggaa tgacgcagag ttgggacgtt aaaattaggg ttgtcacttc gagcgaatgc
     4981 gagaagtctt gaaaaaggtt tgagactttg caaaaccacg tcaattcgtc aattttgtca
     5041 taccggcgca ggccggtatc cagaaaatgg atccccgata ggaccactcg gggatgacag
     5101 aggatggatc taaccagagg gcgccatggc ctgtctccac gggaatggcc caaagaaaaa
     5161 gttatgcaaa gctctcagtt tgtaactgtg aaatgaaagc attaagattc ctattcgctt
     5221 cggcttatcg gaagcgacaa gtttctacag gattgtagat ttttcgtttc tcgcaaccag
     5281 tgaatgcggg ggaaagtcgc cggatggtcg atcgtaaatt taaaaaactt tcaatagttg
     5341 ttccggtttt caacgaagag cgttatctgg aagcggtcat cggcaaggtc gtcgcgcaac
     5401 cgctgcccgg cggtctcgaa aaagaattga ttctgatcaa cgacgcctcg caggataatt
     5461 cctggaatat catgcaggcg ctgccggaga aatttccttc cgttacaatg cagttgataa
     5521 acaagacggt taatgaaggc aagggcgcgg ccctccggga cggctttgct aaaatcaccg
     5581 gggatatcgt cattgttcag gatgccgatt tcgagtatga tcccgccgat tatcccaaat
     5641 tgttgcagcc gatcctggat gacaaagcgg atgtggtgta cggcagccgc ttcatcggcg
     5701 agcctcaccg cgttttgtat ttctggcatc aggtggccaa caatatcctg acaactattt
     5761 ccaatatgct gaccaatctt aacctgacgg acatggaggt ctgttacaag gtttttatcc
     5821 gcgaggttgc ggatcgtctg aaactgaaaa gcccccgttt cggcgttgag cctgaaatta
     5881 cggccaaaat cgcccgtatg cgcttaaacg gcaggcattt gcgggtatac gagaccggta
     5941 tttcctatgc cggccgcacc tacgaggaag ggaaaaaaat aaactggaag gatgcggtat
     6001 cggccatttt ccagattatc cgtttccggt tcatggatta ggtatttttg acgccgagtt
     6061 atcacggccg cttcccgtag ccagcctctt ggtccgggag gtttttcttt tccaaatcat
     6121 ttcaataaca tcaaattttg tgttgcggct gcttcatttt ccaggccggt caatttcagc
     6181 atgatatgaa tatgattgat tatttaaata gagcgattcg cgtcgatttt ctacaatttt
     6241 atcttgacaa tgagcacaat cttatgacga tataacgcat taaaatttat tggattaaca
     6301 aggaggttga gaagcaacgt tatggctaaa tatgtatact ctttctcgga aggatacggc
     6361 aaaagcaaga atcttttggg cggcaagggc gtcggccttg cagagatggc tcatcttaaa
     6421 attcctatcc ccccgggttt tacaattaca acagaagttt gcatggaata tttgagaaat
     6481 aaaggccgtt atccgcaggg attgaaagaa caggtcaaca aagctctgga caaaatggaa
     6541 aaagaaacgg gcatgagatt cggcgatccg aaaaatcctc tcttgtgttc agcccgttcg
     6601 ggagcgcgct ccagcatgcc cggcatgatg gagactgtgc tcaatatcgg tctgacgtca
     6661 aagacgattg ccggcatgat cgaaaaaacc aacaatcccc gtttcgttct ggattcgtac
     6721 cgtcgtctga ttcagatgta ttcggatgtc gtgatggaaa aagccgaagg aatcgagccg
     6781 gaagagggaa tgggcgtccg tcagcagctg gaaagggaaa tggctgcgat gaaagaacgc
     6841 cggaatgtca agctggatgt cgatttgacg gacaaagatc tggcccaact ggctgaaatt
     6901 tacaaggcca aagtcaagga agtgctgggc aaaccgtttc cggatgatgc catggaacag
     6961 ttgtggggcg cgatcggcgc ggttttcaag agctggaacg gcaagcgcgc cgtttcttac
     7021 cgccggatag aaggcattcc cgatgactgg ggcacagcag tcaacgtgca ggcgatggtt
     7081 ttcggcaaca tgggcgaaaa ttccgccacc ggtgttgcgt ttacccgcaa tccggccacc
     7141 ggcgaaaaca aattctacgg cgaatggctg cccaatgcgc agggtgaaga cgttgtcgcg
     7201 ggaatccgaa cccccaatcc gatcaacgag gaaacccgcc agtcggatga tgtcgacggc
     7261 catccttctc tggaaaaagc catgcccaag gcttataccg aaatcaaggc gatccgcaac
     7321 aaactggaag cgcacttccg tgatatgcag gacctggagt tcaccattca ggacggccgc
     7381 gtctggatgc tgcagacccg taccggaaag cgcaacggtc ccgccgcggt gcgcatggcc
     7441 gtggaaatgt acaaaagcaa aatgatcagc gcagaagaag cgatttcccg tgttactccg
     7501 tcacagcttg atgaaatgct gcatccgatg gttgatccga aagccgaagc cacagcggaa
     7561 tttctggtca agggtcttcc ggccggtccc ggcggcgccg taggccaggt tgcgtttacc
     7621 tccgacgtgg cgatggaatg gaggaaggaa ggcaaaaaag tcattctggt tcgcgaagaa
     7681 acgaatcccg aagatgtgga aggcatgcgc gcggctgaag gcgtgcttac ggcccgcggc
     7741 ggcatgacgt cgcatgcggc gctggttgcc cgcggctggg gcaagtgctg tatcgtcggc
     7801 gccggcacgc tgcatgtgga cattgcgcaa aagatcatga aggtggacgg caaggcggtg
     7861 cgcgaaggcg attttgtcac gctcaatgga accaagggac gcgcttatat tggctcgctg
     7921 ccgatgatca gcggcgcgga aaatgatgtc aattttgccg cttttatgaa gctgtgcgat
     7981 tcattacggc gtttgaagat ccgcaccaat gcggaaacgc cgaaagacgc cgccaaggcc
     8041 gtacagttcg gcgccgaagg catcggactg ttccgcacgg aacacatgtt ctacggcgag
     8101 aacagcgaac agccgctgtt ctatctgcgc aaaatgatca ttgcggacac ggaggaaaaa
     8161 cgccgcgcgg cgctggatga attgttcccg ttcgtcaagg gcttcattaa aaagaccatc
     8221 gaagccatgg gcggaagacc ggtcaccatc cgcctgctgg atccgcccct gcatgaattt
     8281 gtgccggaga atcctgagga acgcgcgcgt ctcgcggaga gcctcggtat ttcggttgag
     8341 aaactcaacg gccgcgccga aggtctgcat gaaaccaatc cgatgatggg ccatcgcggc
     8401 gtccgtttgg gcatcagtta ccctgaagtc agcgaaatgc agattcgcgc cattctggaa
     8461 ggctcggcgg agctgatcaa ggaaggcaag aaagcgttac cggaaatcat gattccggtt
     8521 gtctgcgatg tggccgaatt cagcaatcag gcggctctgg tgaaaaaggt ttatccggaa
     8581 gtgctggcca aatacggcct taagaaaatg tcctacatgg tggggacgat gatcgagatt
     8641 ccgcgcgcgg ctctgctggc ggacaagatt gccgaagagg cggaattctt cagtttcgga
     8701 acgaacgatc tgacgcagat gacgtacggc ttcagccgcg acgatgtcgg cgcgttcgtg
     8761 cccggttatc tggcgaagaa aatcattccc gttgatccgt tccagattct ggatcagacc
     8821 ggcgtcggcc agttgattca gatgggcgtg gaaagaggtc gcagcgcgcg tcccggtctg
     8881 aaggtcggga tttgcggcga gcacggcggc gaaccgtcga gtgtcatgtt ctgccatcgc
     8941 gtgggcatgg attatgtcag ctgttcgcct taccgcattc cgctggcgcg tctggctgcc
     9001 gctcaggctg cggtcgaaga gaaaatgagc tcgaagccca aaaccaaggc caaagctaaa
     9061 gccaaaacaa aggccaaaac gaccgtgaag gcaaaagcga aagtgaaaaa agcgaaaaag
     9121 tcacgcaagt aattaaccat agagcgcccg gtgacaagat cgggcgctca gattaattca
     9181 aaaaggaaaa atattttatg gaattagctg taaaaaacaa aaaattgctg agttgggtca
     9241 aagaaatgaa acagctatgc gcacccaaaa aagtgtattg gtgcgatggt tccaaaaagg
     9301 aatacaacac attgatggcg cagatggtga aaagcggtgc ggcgatcaag ctgaagaaga
     9361 gaaaaaactg ttttctgttc cgttccgatc cttcggatgt ggcgcgtgtg gagaaccgca
     9421 cgtatatcag ctatcccgat caggttgacg ccggacccac caataactgg gttgctcccg
     9481 atgcattgaa gaaaacgatg aaaaatcttt acaagggatg catgaaggga cgcacgatgt
     9541 acatcatccc cttttccatg gggccgataa attcgccgat tgccaaaatc ggcatcgaaa
     9601 tcaccgacag tccctacgtt gtctgcaata tgcacacgat gacccgggtg ggtaaaaagg
     9661 ttctcaaaaa actcggcgat acgggggagt ttattccctg catgcattcc atcggcgctc
     9721 cgctggaaaa aggacagaag gatgttgcct ggccctgtgc gccgattgag aaaaagtata
     9781 tcagccattt cccggatgag aacctgatct ggtcgtacgg ttccggctac ggcggcaacg
     9841 ccctgctggg caagaagtgt ctggcgctgc gcattgcgtc ggcgatggcc aaacgcgagg
     9901 gttggatggc cgaacatatg ctgattctga gactgacgaa tcccaaaggc aaacagtatc
     9961 acattgccgc ggcttttcct tccgcatgcg gcaagaccaa tctggcgatg ctgcagccga
    10021 ccatcaaagg ctggacgtgc gaatgcatcg gcgacgacat tgcctggatg aaaatcggtc
    10081 ccgatggaag gctctacgcg atcaatccgg aagccggatt cttcggcgtg gcgccgggaa
    10141 catcctacga ttccaatccc gtggcgatgg atac
//
LOCUS       DAONQZ010000076        10172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_7226_length_10172_cov_16.049521, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000076 DAONQZ010000000
VERSION     DAONQZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7226_length_10172_cov_16.049521"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..>2271
                     /locus_tag="PLR13_09945"
     CDS             197..>2271
                     /locus_tag="PLR13_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HQC19702.1"
                     /translation="MEISFDRSEIDQSIVKRFERQVRLYPDQIAVKDAHRSISFAELN
                     TTANRIAVHLLDKRGDSSEAVALLFEHGLTAIIAILAVLKAGKYYIPLDPSYPRTAIE
                     HMLKDSQAAVILADQTTMDLAGELTAGGITVINADAIDGEADNREPDATIAADAYACI
                     LYTSGSTGLPKGILHTHRTILHHIWSHSRTFGITGKDRQALLLSYSFAASISEIFGAL
                     LNGATLVMNYVKKTGMDHLAKWLQSEKITTFKLPISLFRLFLNTLKGPPRFPDLRLII
                     LGGDKLLRTDVELFRCFFPRDCLLVNRLASTEAFTMTRFFIDQSTALTDQVVPVGYPD
                     PDKEILILDDNAQPAPEGETGEIVVAGRYLSPGYWRLPELNEKKFRTRANHEGQRLFY
                     TGDMGRLRPDGCLEHFGRKDDLIKIRGYRIELSSVEAALNDLKEVREAIVAVQEAGKS
                     IGRKKLVAYLIPAHPAGLSVSQLRIKLAKKLPNYMIPANFVILEKFPLTSSGKIDRLS
                     LPTTEKNRPNLDTPYVAPRTPAEHSIMEIWSEALGLENVGVTDNFFDLGGDSLMLFWV
                     HTHLQDVFDIDIPIIILFKYPTIAALARFIIHRKGLMEGKPPADATAPDVFRPNRPAE
                     PKHAAGSEGSPVRDDIAIIGMAGTFPDAANTDEFWENIKNGVSSVRHYSTQELIDAGV
                     DENLLGDPD"
     assembly_gap    2272..2371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2372..4150
                     /locus_tag="PLR13_09950"
     CDS             <2372..4150
                     /locus_tag="PLR13_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HQC19703.1"
                     /translation="PDGQCRPFDAEARGIVVSNGAGMVLLKRLGEAVRDGDTIHAVIK
                     GSAVNNDGSFKMGFTTPSADGQAAVIEEAQKDAGVHPETITYVETHGTGTSLGDPIEI
                     AALNQAFRRHTWKTGFCAIGSVKSNIGHASRAAGIASLLKTVLSLQHWQIPPSLNFVN
                     PNPEIDFAGSAFFVNTALRPWPGEEGMPRRAGISSFGVGGTNAHLIVEEWQENSVPPV
                     SKPWQLLVLSAKTETALNTTARNLLRHLKQNRDQELADVAYTLQAGRKAFAHRRMLVC
                     RDREEAIALLASPSPPEEELGHCDFSKPSLVFMFPGEGGQYVNMAADLYQQEPSFRAD
                     IDQGAAMFQKSLGLDVRDVLFARKPDLQQADRAMAQKETGRAAIFLVQYAVARLLMSW
                     GVYPEALIGSGIGEYTAACLSGVMTLNEMPTVFAHHHNSDAMKNITLQPPVIPVVSGI
                     TGTWLTLQEATDMAYWSKPLQETPRFSDGLKTLAMFAHCILMDLGPGYPLCAMAGNNH
                     DLKNTLILSALPAANETCLARKTLLRAVGRMWLAGARIDWENLHSQEPRKRIPLPLYP
                     YEKKRYWISRVSAGRIPQTGTIRFTE"
     gene            complement(4155..5459)
                     /locus_tag="PLR13_09955"
     CDS             complement(4155..5459)
                     /locus_tag="PLR13_09955"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HQC19704.1"
                     /translation="MTPVTVGVFGLIVMAVLIMIRVPIGCAMGIVGLAGFSILSSPDA
                     AFSMLGMVVYQTVSSYALTVIPLFFLMGQLAGQSGMGVELYRAVHRWTGFLPGGLAMA
                     TVGACAGFSAISGSSLATAATIGAVALPEMKRFRYDDALATGCVAAGGTLGILIPPST
                     VMVVYAILTGQPVGTLFIAGIIPGIILTGLFLLTIFLWSRKRHGISHPDSRFSFAEKW
                     ESLRDTGSVLLTFALVVGGLYGGWFTPTEAAAVGALIVLVIISAKKRLTWKLLAGSLT
                     ETALTTAMIFAILIGANIFGYFMTLSDLPATLTAWVTDTGMNRYATLGIICLVYVVLG
                     CILEGFAIMFLTLPIVFPMVTAMGFDPIWFGVLMILLIEMGLITPPVGINVFIISGIA
                     KNVPMATIFRGIAPFFIAILICVVLLFLFPQLALFLPATMAG"
     gene            complement(5456..5944)
                     /locus_tag="PLR13_09960"
     CDS             complement(5456..5944)
                     /locus_tag="PLR13_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HQC19705.1"
                     /translation="MKRVFSCIHKTATGLNGVAAGIIFILMILTAANVLLRKLTGISI
                     TGMTEISELMMIILVFFALAQAEIMDRHVSVSLLTDRLTSGARKYLAVITSLANALFF
                     ALITAAAIVYGSSLRLSGDVSMDLQIPKYPFAYVIAFGAAVLSFVFFKKTLASIKDEK
                     VL"
     gene            complement(5958..6974)
                     /locus_tag="PLR13_09965"
     CDS             complement(5958..6974)
                     /locus_tag="PLR13_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HQC19706.1"
                     /translation="MKKHYLILLMICGVLLTAGPVAAQDAVELKLAHFIPSTHIQHQK
                     TFLPFAQNVEKLSGGKVKIKIYPAGTLGGAVQLPDAVKTGIADIALIFPSMTSGRFPR
                     FSAYDLPFLFDNPQQAIQVAYDLYERWFAEDFKDYKLLWIYCTDMGQLYSVDKPILTM
                     EDFKGMKLRSPSASMSDALKRLGANPVGMPITEMHMALAKHVIDGALTPNTVVADFKL
                     YDQIKHITQTDFYISIMAVVMNKKKYEALSPAAKQAIDNAAGKSWGLHATGVYDDYSR
                     ETVNQLRTSGNVKIHDMAPDERKKIRNQLAGMESDWIRQNSEKGIPAREMMDAIHRAS
                     AKIK"
     gene            7126..7566
                     /locus_tag="PLR13_09970"
     CDS             7126..7566
                     /locus_tag="PLR13_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19707.1"
                     /translation="MMDIEALIPHREPIKVITEILELDDDSGSAAAVVNERWPLCDGK
                     AVCALVLIEAIAQTSALIEGHKRMKKSMGGTKGWLVGIKTAEFRTATVPLGTRITVTV
                     KGLSSFDEYGVIEGVVKAGDDILLSAVLQAIRFNGHGVEQDIRG"
     gene            7544..8305
                     /gene="fabG"
                     /locus_tag="PLR13_09975"
     CDS             7544..8305
                     /gene="fabG"
                     /locus_tag="PLR13_09975"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQC19708.1"
                     /translation="MNKIFEDDVPIMEEKKTALVTGASRGIGRATAVELAKNGYFVVI
                     NYKSNEAAAAETLVQVEQAGSGGTLCRFDVANAEEVRTALDQIIKTHKRVDVLINNAG
                     ITADGLFVMMDEEDWLNVVNTTLNGFYHVTRPVLREMIRKKRGAIVSVSSVSSILPNR
                     GQANYAAAKAGINAATRSLSKEVARFGIRVNVVAPGPVETDMIRSMPMDDIKKIIPMA
                     RVGRPEEVAKVICFLCSDDASYVTGQVIGVNGGMF"
     gene            8490..9350
                     /locus_tag="PLR13_09980"
     CDS             8490..9350
                     /locus_tag="PLR13_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19709.1"
                     /translation="MPKTDHCQGPLKDPSEDGYLPVDHQDFRIIEREGINYYLVDLRK
                     AAMNALRDIIEGCAIVEQECWKPQEDFSHYIQKCDMLSYAVLARRIIGFCAGSLFVHD
                     AIFIYSNDETMVLREFHHRNIARNMVFCNMRWILQSRHDEGIKHYAFMSISGNPRIVN
                     GYYNHSFLIKILFDCSFKASDKLIRALDAYRHQYKISLVHEDYPFCLKNMFPGSNTFN
                     PSDQRFQFLKRVKRNMPADFDHMERGDAFAFLVKLPAWFTRTFVFGLMVLAFGRRFFS
                     RRGIGFFAGN"
     gene            complement(9403..>10172)
                     /locus_tag="PLR13_09985"
     CDS             complement(9403..>10172)
                     /locus_tag="PLR13_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQC19710.1"
                     /translation="DWGDEKEYWEFTVEGSRLIFDEAVKQNARIVLASSIAVYGDNIY
                     RQACPEDTPYGNTLGPYSRTKQAQEKLAWNYYRNKGMKLTVVRPANVYGPRSGPWLHD
                     VVNVLRSGSPGLISGGQMNAGLAYVDNVADILILAGTFEKALGNVYNACDGLDVTWKR
                     YFTDIANIIGVGNPKSLPRPLAVLGAYLLEGIYKLLGIKKRPLITREALNLVGSDNRI
                     PIDKIRRELGYEPVISYAEGLKHIREYLHQDPPCKKT"
BASE COUNT         2422 a         2580 c         2755 g         2315 t
ORIGIN      
        1 gggcgcgcag ttgaagggtc agccgtagca cttcccgaaa atcttcattc taaaatttac
       61 gattttatgt tagaccatgt ccggttttgt tgaccgcaaa tacggtactt tcatgcagga
      121 tactttttaa aacctcaatt taaccgattg cctttacgtt gggggcaaat caattattac
      181 ctgttgtggt aagaatatgg aaatttcatt tgacagaagt gaaatcgatc aatccattgt
      241 caaacgtttt gagcggcagg tccggctgta tccggatcag attgccgtaa aagacgcaca
      301 ccggtccata agctttgccg aactcaacac aaccgcaaac cgcatagccg tgcatctcct
      361 ggataaacgc ggggactcgt cggaagccgt tgcgcttttg tttgaacatg gcctcacggc
      421 gatcattgcc attcttgctg ttctgaaggc gggtaaatat tacatcccgc tggacccgtc
      481 gtatccccgg acggcgattg agcacatgtt aaaggactcg caggcggcgg ttattctggc
      541 cgaccagacc accatggatc ttgccgggga attgacggcg ggcgggataa cggtgatcaa
      601 cgccgatgcg atagacgggg aggcggacaa ccgcgaaccg gacgcgacca ttgcggcgga
      661 tgcatatgcc tgcattcttt acacgtcggg ttccaccggc cttcccaagg gaattctgca
      721 tacccaccga accatcctcc atcatatctg gagtcacagc cggacgtttg gaattaccgg
      781 caaagaccgg caggctctgc tgctttccta cagttttgcc gcgtccattt ctgaaatatt
      841 cggcgccttg ctcaacggcg caacgctagt catgaattat gtcaaaaaga cgggcatgga
      901 tcatctggca aaatggctgc aaagtgaaaa aattacaacg ttcaagctac ccatttctct
      961 gttccggctg tttctcaata ccctgaaggg tccgccccga ttcccggatt tgcggttgat
     1021 catcctggga ggcgataagc tgctgcggac tgatgtggaa ctgttcagat gtttctttcc
     1081 tagagactgt cttcttgtca accgtctggc gtcaaccgaa gccttcacca tgacccgctt
     1141 cttcattgat cagtcaacgg ctttgacgga tcaggtcgtt ccggtggggt atccggatcc
     1201 cgataaagag attctcattc tggatgataa cgctcagccg gcgcccgaag gcgaaaccgg
     1261 ggagattgtt gtcgccggcc gttatctttc gccggggtac tggcgtctcc cggaactaaa
     1321 cgaaaaaaaa ttccggacac gcgcgaatca tgaaggacaa cggctctttt ataccggaga
     1381 tatggggcgc ctgcggccgg acggttgtct ggagcacttc ggacgcaagg atgatctgat
     1441 caaaatccgc ggctacagaa tcgagctctc cagtgtggaa gcggccttga acgacttgaa
     1501 agaagtccgg gaagcaatcg tcgcggttca ggaagcgggg aaaagcatcg gcagaaaaaa
     1561 actggtcgcc tatctgattc ctgcacatcc ggccgggtta tccgtcagtc aactgcgcat
     1621 caagctggcg aagaaattac ccaactatat gattccggca aacttcgtta tcctggagaa
     1681 gtttcccctg acatcgtccg gcaaaatcga ccgcctgtcc ctgccgacga cggaaaaaaa
     1741 ccggccgaac ctggacacgc cgtatgtcgc gcccagaacg ccggcggaac acagcattat
     1801 ggaaatctgg tctgaagccc tcggcctgga aaacgtcggg gtgaccgata attttttcga
     1861 tctcggcggg gattccctga tgctcttctg ggtgcacacc caccttcagg atgtttttga
     1921 tatcgacatc ccgatcatca tcctgtttaa gtatccgacc atcgcggcgc tggcgcgctt
     1981 tatcattcac cggaaaggat tgatggaggg caaaccgccc gccgatgcaa cggcgcctga
     2041 cgttttccgt ccgaaccgcc cggcagagcc gaaacatgcg gctggttccg aaggatcgcc
     2101 tgtccgggac gatatcgcca ttatcggcat ggccggcacg tttcccgatg ccgcaaacac
     2161 ggacgaattc tgggagaaca taaaaaacgg ggtgtcgtcc gtgcgccatt attcgacgca
     2221 ggaactgatt gatgccggcg tggatgaaaa tcttcttggc gatcccgatt annnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgacggt caatgccgcc cctttgatgc
     2401 ggaggcgcgc gggatcgtgg tcagcaatgg cgccggaatg gttttgctca aacgactggg
     2461 cgaagccgtc cgcgacgggg ataccatcca tgcggtgatc aaagggtcgg cggtcaacaa
     2521 tgacggatcg ttcaagatgg gttttacgac gcccagtgcg gacggacagg ccgccgtcat
     2581 cgaggaggcc cagaaagatg ccggggtcca tcccgagacc attacgtatg tagaaacgca
     2641 cggcaccgga acaagccttg gcgatcccat tgaaattgcc gctttaaacc aggcctttcg
     2701 ccgccatacc tggaaaacgg gtttttgcgc catcggttcc gtcaaatcaa atatcggcca
     2761 tgccagccgc gccgccggta tcgccagcct gctgaaaacc gttttatcgc ttcaacactg
     2821 gcagattccc cccagcctga attttgtcaa tccgaatccg gaaattgatt ttgccggcag
     2881 cgcctttttt gtcaacaccg ctttgcggcc ctggcccggt gaggagggaa tgccgagacg
     2941 ggccgggatc agttcgttcg gcgtgggagg aacaaatgcc catttgattg tggaggaatg
     3001 gcaggaaaac agcgttcccc cggtatcaaa gccgtggcag cttctggttt tgtcggctaa
     3061 aaccgaaaca gccctgaaca caactgcccg caatctattg cgccacctga agcaaaatcg
     3121 cgatcaggaa ttggccgatg tcgcttatac attgcaggcc gggcgcaagg cctttgcgca
     3181 tcgccgcatg ctggtttgcc gggatcggga agaggcgatc gcgcttctgg cgtcaccatc
     3241 gccgccggaa gaggagttgg ggcactgcga tttttccaag ccgagcctgg tctttatgtt
     3301 tcccggcgaa ggcggacagt atgtcaacat ggccgctgat ctgtatcagc aggaaccctc
     3361 gttccgtgcg gatatcgatc agggcgccgc gatgtttcaa aagtcgttgg ggctggatgt
     3421 tcgcgacgtc ttgtttgcgc gaaagcccga tctgcaacag gcagacaggg ccatggcaca
     3481 aaaagaaacc ggccgcgcgg cgattttcct cgtccagtac gctgtggcgc gtctgctgat
     3541 gagctggggc gtctatccgg aggcgctgat cggcagcggt atcggtgaat acacggcggc
     3601 ctgcctgtcc ggggtcatga cgctgaatga gatgcccact gtgtttgcac atcatcataa
     3661 ttcagacgct atgaaaaata ttacccttca gccgcctgtt attcccgttg tttccgggat
     3721 aaccgggaca tggctgacgc tgcaggaggc gacggacatg gcttactgga gcaaaccctt
     3781 gcaggagacg ccccgtttca gcgacgggct gaagacattg gcgatgtttg cccattgtat
     3841 cctgatggat ttgggaccgg gatatccgtt gtgcgcaatg gccggcaaca atcatgattt
     3901 gaagaataca cttattttat cggcgctgcc tgcggcgaat gaaacgtgtt tggcgcgcaa
     3961 gacgctgctt cgtgccgtgg gcaggatgtg gctggccggc gcccggattg actgggaaaa
     4021 tttacattcg caggaaccga gaaagcgcat tccgctgcct ctttatccct acgagaaaaa
     4081 gaggtattgg atttccagag tctctgcagg acggattccg caaaccggta cgatccgctt
     4141 taccgaataa agggtcatcc ggccatcgtg gcgggcagaa acagtgccag ttgagggaag
     4201 aggaataaca gaaccacaca gatcaggatg gcgataaaaa agggagcgat gccccggaag
     4261 attgttgcca tgggaacatt cttggcgatt ccggaaatga taaagacatt gattcccacg
     4321 ggcggcgtga tcaggcccat ctcaatgagc agaatcatga gcaccccgaa ccagatcggg
     4381 tcgaacccca ttgccgtgac catcggaaac acgatgggca gggtgagaaa catgatggca
     4441 aatccttcca ggatgcaccc cagaacaaca tacaccagac aaatgatacc cagcgtcgca
     4501 taccggttca tgcctgtgtc cgtgacccag gcggtcaatg tcgccggcaa atcgctcagc
     4561 gtcataaagt agccgaagat atttgctccg atcagaatgg caaaaatcat ggcggtggtc
     4621 agggctgttt ccgtgagcga accggccaaa agcttccagg taagtctttt ttttgcagaa
     4681 ataatgacca gaacaatcaa cgctcccacc gccgccgctt ctgtcggtgt gaaccagcct
     4741 ccgtaaagtc cgcccaccac cagcgcgaag gtcaaaagaa cgcttccggt gtcccggagg
     4801 gattcccatt tttcagcgaa agaaaatcgg gaatccggat gactgatgcc gtgacgtttc
     4861 ctgctccaca gaaagatcgt caaaagaaaa agccctgtga ggatgatgcc gggaatgatg
     4921 ccggcgataa agagcgttcc gacgggttgt cccgtaagaa tggcgtagac caccataacc
     4981 gtgctgggcg gaatcaaaat gcccagtgtt ccgcccgcgg ccacacatcc ggtcgccagc
     5041 gcgtcatcgt atcggaagcg tttcatttcc ggcaatgcaa ccgcgccgat ggtggctgcc
     5101 gtcgctaaag aggagccgga gatcgcggaa aatccggcgc acgcgccgac cgttgccatc
     5161 gccagcccgc cgggcagaaa gccggtccag cggtgaacgg cacgatataa ttcaacgccc
     5221 atgccggatt gaccggccag ttgtcccatc aggaaaaaga gcggaatgac ggtcagggcg
     5281 taggaggaga cggtctgata gacgaccatg cccagcatgg aaaaggccgc gtccggagag
     5341 gacaggatgg aaaatcccgc cagaccgaca atgcccattg cgcagccgat cggaacgcga
     5401 atcatgatga ggacggccat gacgatgagg ccgaatacgc cgacggttac cggggtcata
     5461 gcactttttc atcctttatc gatgccaggg tttttttaaa aaagacgaaa ctgagaacgg
     5521 cagcgccgaa ggcaatgaca taggcgaagg gatatttggg aatctggaga tccatggata
     5581 cgtcaccgga tagtcttagc gaagagccgt aaacaatcgc cgccgccgtg atgagcgcaa
     5641 aaaacagggc attggcaaga gacgtgatga cggctaaata tttcctggcc ccggaggtca
     5701 ggcggtctgt caaaagggaa acgctgacat ggcgatccat gatttccgct tgggcaagcg
     5761 cgaaaaagac aaggatgatc atcatcaatt cgctgatttc cgtcattccg gttatgctga
     5821 tgcccgtcag ctttctcaaa agaacattcg ccgcggttag aatcatcagg ataaaaatga
     5881 tccccgcggc gacgccgttt aagccggttg ccgttttgtg tatgcaggaa aagaccctct
     5941 tcacaaccgc acgcgcccta ttttatttta gccgaagcgc ggtgtatggc atccatcatc
     6001 tcacgtgcag gaattccctt ctcgctgttt tgccggatcc agtcgctttc catgcctgcc
     6061 aattggttcc ggattttctt tctttcgtcc ggggccatgt cgtgaatttt cacgttgcct
     6121 gacgtccgca gctgattgac agtttccctg gagtaatcgt catacacacc ggtggcgtga
     6181 agcccccagg attttccggc cgcgttgtca atggcctgtt tggcggcggg agacagcgcc
     6241 tcatatttct tcttattcat gaccacggcc atgatggaaa tataaaaatc cgtttgggta
     6301 atatgtttga tttgatcgta gagtttgaag tccgcgacaa ccgtattggg ggtcagcgcg
     6361 ccatcgataa cgtgttttgc aagggccatg tgcatttccg tgatcggcat tcccaccgga
     6421 ttggcgccca gccgcttcag ggcgtcgctc atggacgcgg aaggcgaccg caatttcatt
     6481 cccttgaaat cctccatcgt caggatcggc ttatcgacgg aatataactg tcccatgtcg
     6541 gtgcaataaa tccagagcag tttatagtcc ttgaaatctt cggcaaacca gcgttcataa
     6601 aggtcgtaag ccacctggat ggcctgctgc ggattatcga agaggaacgg cagatcatac
     6661 gccgaaaatc ggggaaaccg gccggaggtc atggacggaa agatcagagc gatatccgcg
     6721 atacccgttt tgacggcgtc cggaagttgc accgctcccc cgagagtgcc cgctggatag
     6781 attttaatct tcaccttgcc gccggacagt ttttccacat tttgggcgaa gggcaggaag
     6841 gtcttctggt gttgaatatg cgtggacggt atgaaatgcg ccagcttcaa ttccaccgca
     6901 tcctgagccg cgacagggcc ggccgtcaat aataccccgc agatcatcaa taatatcaaa
     6961 taatgctttt tcattatttt atttctcccc tgaagatttt ttatgattat agaaaaaaaa
     7021 caattcgtgt caatgccttt tgcacagcag gccctgttga atgttttgat tgacttctgc
     7081 agtatttttt aatttattga aagaaaaaaa tgaaggaatg gatcgatgat ggacatcgaa
     7141 gcgctgatac cgcaccgcga gccgattaaa gtcatcacgg aaattcttga gctcgatgac
     7201 gattccggtt cggccgcggc cgtggtcaac gaacgctggc cgctgtgcga cggcaaggcg
     7261 gtgtgtgcgc ttgttttaat tgaagccatt gcccagacgt ccgctctgat cgaagggcat
     7321 aaacggatga aaaagagcat gggcggcaca aaagggtggc tggtcggcat caaaaccgcc
     7381 gaattcagga cggcgacggt gcccctgggt acgcggataa cggttaccgt taaaggcctt
     7441 tcttcatttg atgaatacgg tgtcatcgag ggtgtggtaa aggcgggcga cgacattttg
     7501 ctgtcggccg tgcttcaggc aatccgattc aacgggcacg gggttgaaca agatattcga
     7561 ggatgatgtg ccgatcatgg aagaaaaaaa gacagccctt gtaacgggcg caagccgggg
     7621 gataggacgg gccaccgccg tggaactggc caaaaacggt tactttgtgg tgattaatta
     7681 caaatccaat gaggcggcag ccgccgaaac gctggttcag gttgagcagg caggatccgg
     7741 cggaacattg tgccgatttg atgtggcaaa cgcagaagag gtccggacag cgcttgatca
     7801 aatcataaag acgcacaaaa gagtcgatgt cctgattaat aatgccggca tcacggccga
     7861 cggccttttt gtgatgatgg atgaggagga ctggctcaat gtcgtcaaca cgacgttgaa
     7921 cggattctat cacgtgacca ggcccgtctt gagggaaatg attcgaaaga aacgcggggc
     7981 cattgtttcc gtttcttctg tctcctcgat cctgccgaat cgcggtcagg ccaattatgc
     8041 tgcggccaag gccggcatca acgccgccac gcgttcgctc tccaaggaag tggcgcgctt
     8101 cggtattcgg gtgaatgttg tcgcgccggg tcccgttgaa acggacatga tccgcagcat
     8161 gccgatggac gacattaaaa aaatcatccc catggcgcgc gtgggccggc ccgaagaagt
     8221 cgccaaagtg atttgttttt tatgctccga cgatgcgtct tacgtgacag ggcaggtcat
     8281 cggcgtcaac ggcgggatgt tttaattttt cacaaaaatc cgatgataaa gaaaaatgct
     8341 tcttgcttct gaaaaaatgc ccgtcatgac cctcgcggaa aaattttaaa tagcatgtga
     8401 tttgctgagt cgtcatgttc ctgtcggagc gacaaaaaaa ccgtcaggta aagtgatttt
     8461 ccgggatggc cgaaccagtg ggatgcgtta tgccaaagac cgatcattgt cagggaccgc
     8521 taaaagatcc atcggaggat ggttatttgc ctgttgatca tcaggatttc cggatcattg
     8581 aaagagaagg catcaactac tatttggtgg atcttcggaa ggcggctatg aatgccttaa
     8641 gggacatcat cgagggatgt gccatcgtag agcaggagtg ttggaaacct caagaggatt
     8701 tttcccatta tatccaaaaa tgcgacatgc tgagttatgc cgtgctggct cgccggatta
     8761 tcggtttttg cgcgggcagt cttttcgtcc atgacgccat ctttatctac agcaatgatg
     8821 agaccatggt gctcagagag tttcatcacc gtaatattgc cagaaacatg gtcttctgca
     8881 atatgcgctg gatattgcaa agcaggcacg atgaaggcat caaacactac gcgttcatgt
     8941 cgatttcagg caatcccaga atcgtgaatg gatattacaa ccatagtttt ctaataaaaa
     9001 tcctattcga ttgttccttt aaggcgtcgg ataaattaat ccgtgccctg gacgcctacc
     9061 gtcatcaata caagatatcg ctggtgcatg aggattatcc cttttgtctc aaaaatatgt
     9121 ttccgggatc gaacaccttc aatccgtcgg atcaaagatt tcaatttctg aaaagggtca
     9181 aaaggaatat gccggctgac tttgatcaca tggagcgggg agatgccttt gcgtttctgg
     9241 tgaagttgcc ggcatggttt acacgtacgt ttgtcttcgg cctgatggtt cttgccttcg
     9301 gcaggcggtt tttctcccgt cgcggcatag gcttttttgc cgggaattaa ctctgaataa
     9361 acgcgaacga cttcaaaacg gtcatgactc caaaaaacgc gtttaagttt ttttacacgg
     9421 cgggtcttga tgaagatact cccgaatatg ctttaatcct tcggcatagg aaatgacggg
     9481 ttcatacccg agctcgcgtc gaattttgtc gatcggtatc cggttgtctg agcccaccag
     9541 attcaaggct tcgcgcgtaa tgagaggacg ttttttaatg cccagcaatt tataaattcc
     9601 ttccagtaaa taagctccaa ggactgccag cggccggggg agggattttg gattgcccac
     9661 gccgatgatg ttggcgatgt ccgtaaaata tcgtttccac gttacgtcca gcccgtcgca
     9721 ggcgttgtaa acgttgccca gtgccttttc gaaagttccc gcaaggatga ggatatccgc
     9781 cacattatcg acataggcca gtcccgcgtt catctgtccg ccggagatca ggccgggcga
     9841 tccgctgcga aggacattca ccacgtcgtg aagccagggg ccggagcgcg gcccgtagac
     9901 gttggccgga cgaaccacgg taagtttcat gcccttgttc cggtaataat tccaggcgag
     9961 cttttcctgc gcctgttttg tcctgctgta gggtcccagt gtgttgccgt aaggtgtatc
    10021 ttccgggcag gcctgcctgt aaatgttgtc gccataaacg gctatgcttg aggcgagaac
    10081 gatccgggcg ttttgtttta ccgcctcatc aaaaatcaaa cggctgcctt cgacggtaaa
    10141 ttcccagtat tctttttcat cgccccagtc gg
//
LOCUS       DAONQZ010000077         9696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_7661_length_9696_cov_14.410435, whole genome shotgun sequence.
ACCESSION   DAONQZ010000077 DAONQZ010000000
VERSION     DAONQZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7661_length_9696_cov_14.410435"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1821
                     /locus_tag="PLR13_09990"
     CDS             115..1821
                     /locus_tag="PLR13_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19711.1"
                     /translation="MTIAVKQQSGKKSINKRKVLQNDLSILARDIIANAGVGMYIVQD
                     GKFVYVSELYQKITGFTEEELIGTYPLDNIHPDDMKTVREKAIKCLKEGNCNPYEYRF
                     ITKSGEIIWVLEAVTSISFKGERAALGSFINITERKLIEKKLHHEEQLFRTLTDQSSD
                     IIAIIDREGVITYENQAIEPVLGLKVDEQMGGRGFDNLHPDDVPFVSNELKRLFADPD
                     ASASRSEVRLRHKNGQWLVFEFMASGLVNNNVVEAVMINLRDITERKRAEESLRFSEE
                     KYRTILENIQEGYFEVDLAGNLTFYNDPVCEIMGYTREELMSMNYKKYTGNEMAVKVY
                     QAFNKVYLSGNSTKEIDWQIIRKDGARRYIEASVSLQKDFSGHPKGFKGIIRDITERK
                     EMDQKLNHLATHDALTGLPNRILFMDRLQVALAQSRRNRNKLAVMMLDLDHFKDVNDT
                     LGHLAGDKLLQEVGNRLTDILRHNDTVARLGGDEFVVLLSDLERMEYAAGVAKVILKA
                     FGKPFLLADHEITPNVSIGIAFFPDDCDDVESLLQKSDMAMYSVKTHGRNSYKFYSHI
                     AS"
     gene            complement(1835..3445)
                     /locus_tag="PLR13_09995"
     CDS             complement(1835..3445)
                     /locus_tag="PLR13_09995"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQC19712.1"
                     /translation="MKHTKFIFITGGVLSSLGKGLAAASISALLESRGLKVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCSTCMGKSNNLTTGQVYYAVIT
                     KERRGDYLGKTVQVIPHITNEIKDFIRDTAAGFDVAIVEIGGTVGDIESLPFLEAIRQ
                     FRNEVGRENAIFIHLTWVPLIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLS
                     DDIKSKIALFCNVEVHSVFTAKDVSCIYEVPLIFHREGLDAKIVDLLNIWTGQPKLDV
                     WEDVVDKFHNPLHDVTIGIVGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFIDSEKI
                     EAEGLGKHLDDIDAVLVPGGFGIRGIEGMILAIQRAREKKIPFFGICLGMQMAVVEYA
                     RNVCKLEKANSSEFDPETSYPVIDLLPDQRHVKEKGASMRLGGWPCLVEADSFACKAY
                     GQGEISERHRHRYEFNNDYKEALMNQGLRITGISPDGRLAEIVEIKDHPWFLGCQFHP
                     EFKSRPIKPHPLFSKFIEAALNYSRSKT"
     gene            complement(3492..4451)
                     /locus_tag="PLR13_10000"
     CDS             complement(3492..4451)
                     /locus_tag="PLR13_10000"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQC19713.1"
                     /translation="MGTTIWVTKDFAHMSDVASGIVIKKSIELLKRKKEIALGLATGN
                     SPTGLYKHLAKAVNEGRLDAGRIRSFNLDEYVGLPGNNIQQRVMHPESYAYFMIQELF
                     GHLNKKFIETRVPYGSLIDQKMLIRELKDNKSDWEFKGTDAGKSIAIKAKAKSPYLAW
                     IRKDILDGYVRKIKAVGGIDLQIIGVGGRGHVAFHESGIPFQGSSMLLVKLDDNTIAN
                     AVADGHFKTKADSPQYAISMGAELVYKAKTVVLVANGARKTESVTRSILGNMTPEIPI
                     SYGQAYSQKGGELIYVLDRIAAQGLLENTSALKTRGVILKNLA"
     gene            complement(4470..5819)
                     /gene="glmM"
                     /locus_tag="PLR13_10005"
     CDS             complement(4470..5819)
                     /gene="glmM"
                     /locus_tag="PLR13_10005"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQC19714.1"
                     /translation="MGKLFGTDGIRGEANRHPMTPQIAFGVGQAIAHVFKKGGHRSVI
                     IIGKDTRLSGYMLESSLEAGITSMGGYSYLVGVMPTPGIAFVTQSMRADAGVVISASH
                     NPFQDNGLKIFGGDGYKLTDEKEDIIEDLILNDKLSEFLSKGDDIGRAFRLEGVNGRY
                     IVFVKNTFPRNLSMEGMKIVLDTANGATYRVAPDVFHELGADIDVIHKTPNGLNINDQ
                     CGSQHTERLKKTVLENGAAIGLAFDGDGDRLIAVDEKGQEITGDQILVICANVLKREG
                     RLKNDLLVSTVMSNLGLKVACKQYGFKHHASKVGDRYVLEDMLKMGSVIGGEEAGHMI
                     FLDHHTTGDGIIAALQLVAAMIKENKPLSELARMMDIFPQKLINVDVKSKPDIDQIPR
                     LAEAIKQVEKELGDEGRVLVRYSGTQNMCRVMVEGPTDAVTLKYCRQLADIIKSEIG"
     gene            complement(5867..7132)
                     /locus_tag="PLR13_10010"
     CDS             complement(5867..7132)
                     /locus_tag="PLR13_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HQC19715.1"
                     /translation="MDLKPRSLKKINDLLERGVSIPNPETIDIGDDVAIDRISGKGVK
                     IYPGCRIYGKETTISSGCQLGYEAPVTIENCQLAEKVELKGGFFSKSVFLEKSNMGMG
                     AHVREGCILEEEAGGAHCVGLKQTILFPFVTLGSLINFCDCLMGGGTSRSNHSEVGSS
                     YIHFNFTPDADKTTPSLLGDVPRGVMLNQPPIFLGGQGGIIGPVRLGFGNVVAAGSVL
                     RHDYPGDNQLIFEKAPACSVKPFYPAAYPHLERIVENNILYLANLKALEAWYTHVRKP
                     FLETGEYGSLLYAGVMEQLSSARKERLKRLKAMAQKAAAAAEEEKNPERTCARGRKEL
                     HEKMGHIEDLCNGKASPASGKTDELRDTFLNDLESIAGKNKTNYIETIQALPAGISSK
                     GVMWLDGIVRDLGENVSRAVPKLGLLKTF"
     gene            complement(7182..8537)
                     /gene="gdhA"
                     /locus_tag="PLR13_10015"
     CDS             complement(7182..8537)
                     /gene="gdhA"
                     /locus_tag="PLR13_10015"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQC19716.1"
                     /translation="MSVIQDVIAKVKQTDPNQPEFHQAVEEVMETLEPTVKKHPEFVK
                     ANLYERIVEPERAVIFRVPWVDDKGNVQVNRGFRVQFNNAIGPFKGGIRFHPSVNLGI
                     IKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDGEVMRFCQAFMRELYRHIGADVD
                     VPAGDIGVGGREIGFMYGYYKKIRNEHTGVLTGKGMSYGGSLIRPEATGYGCVYFAAE
                     MLATRKMDFKGKTVAVSGAGNVAQYAVEKVNQLGGKVISLCDSSSCIIDEAGIDDKKC
                     SFVLDLKNVKRGRISEYANKFKSECFEGKNVWDVIREQGIKVDIALPCATQNEINGKN
                     AAALVKNGCLCVAEGANMPSTPEAIKIFQDKKILYGPGKAANAGGVATSGLEMSQNSL
                     RLLWTREEVDNRLLIIMRSIHKNCYDTAETYGKKGDYVTGANIAGFTKVANAMLAYGV
                     V"
     gene            complement(8603..9604)
                     /locus_tag="PLR13_10020"
     CDS             complement(8603..9604)
                     /locus_tag="PLR13_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HQC19717.1"
                     /translation="MKHLFNPEDIERKVLLILKILNKNSESAGARVIARKMKEHGVHL
                     SERTVRYHLKSMDERGLTRLIGRRDGRIITDMGMEEINNARVRDKISLSISRIDVLSL
                     KTTFDIKKRRGLVPVNISLFPQKQFRKALAVMKPVFKKKIAVSDLVAVASAGETLGEV
                     TIPEGKIGFATVCSILINGVLLKHGIPMDSKFGGILQVRHGNPLRFVELINYSGSSLD
                     PSEIFIRGKMTSVKDVVKKGEGKILANFREIPALALSLVENIVADFKKIGVNGALSIG
                     NVGNPVCQTNVDINKVGMILLGGLNPIAAAGEEGFDIENKAMSTVMEYETLQRVEDI"
BASE COUNT         2294 a         2401 c         2355 g         2646 t
ORIGIN      
        1 ttatacactc acaggaaact atggtttcat ctattttctg cgacgcaata ttcaaagatt
       61 agttgaatgg acaatgcatt tttaatgtat ttgccgttaa tttaatgtgg ttatgtgact
      121 atagccgtga aacagcagtc gggaaaaaaa tccattaata agagaaaagt tctccagaac
      181 gacctgtcta ttctggccag agatatcatt gcaaacgccg gcgtcggaat gtatatcgtc
      241 caggacggca aatttgttta tgtcagcgaa ttatatcaga aaattaccgg ctttacggaa
      301 gaagaactaa tcggtacata tccgctggat aatattcatc cggacgatat gaaaaccgtc
      361 agagaaaagg cgataaaatg tttgaaggaa gggaactgca acccttacga atacaggttc
      421 attacaaaaa gcggtgaaat aatctgggtg ctggaagcgg tcacctccat tagctttaaa
      481 ggggagcggg ctgccctggg cagttttata aacatcaccg aacgcaagct catcgagaaa
      541 aaattgcacc atgaggagca actgttcagg acacttacgg atcaatcttc ggatatcatc
      601 gcgattattg accgggaagg tgttattacg tatgaaaatc aggccattga accggttctg
      661 ggtttgaaag tcgatgaaca gatgggcggc aggggctttg acaatcttca tccggatgat
      721 gtgccgtttg tctccaatga attaaaaaga ctgtttgcgg atccggatgc ttctgcttcg
      781 cgttcggaag ttcgtctccg ccacaaaaac ggtcagtggc tcgtgtttga atttatggcc
      841 agcggtttgg tgaacaataa tgttgtggaa gcggtgatga tcaacctgcg ggatattacc
      901 gaacggaaaa gggctgagga gtcgcttcgg ttcagcgagg aaaaatacag aaccattctg
      961 gaaaacattc aggaaggata ttttgaggtc gatctggccg gtaatctgac tttttataac
     1021 gatccggttt gcgaaatcat gggctatacc agagaagaat tgatgtccat gaattataaa
     1081 aagtacaccg gtaacgaaat ggcggtgaag gtttatcagg cattcaataa agtttacctg
     1141 agtggcaatt ccaccaagga aattgactgg cagattatca gaaaagacgg agccaggcgc
     1201 tatattgaag cttcagtatc gctgcaaaag gatttttccg gccatccgaa aggattcaag
     1261 ggcatcatcc gtgatattac cgagcgcaag gaaatggatc agaaattgaa tcatctggcc
     1321 acacacgatg cactgaccgg tctgcccaat cgaatcctgt ttatggatcg tcttcaggtt
     1381 gcgctggcgc aatccagaag aaaccggaac aaactggccg tcatgatgct tgacctggat
     1441 catttcaagg atgtgaatga cacgctggga catctggcgg gcgacaaact gcttcaggag
     1501 gtgggcaatc gcctgacgga tattttacgg cataatgaca ccgttgcgcg gctgggcggt
     1561 gacgaatttg tggttttgtt gtcggatctt gaaagaatgg aatacgcagc cggcgtggcc
     1621 aaagtcattt tgaaagcttt tgggaaacct tttttactgg ccgatcatga aattacaccc
     1681 aacgtcagta tcggcattgc cttcttccct gatgattgtg atgatgtcga atcgcttttg
     1741 caaaaatcgg atatggccat gtattcggtt aagacgcacg gacgtaacag ctataaattc
     1801 tacagccaca tcgcgtcttg aaaaccaaac atggttacgt tttggagcgg gaatagttca
     1861 gcgcggcttc gataaatttg ctgaaaagcg ggtgaggttt gatcggtctg gatttgaatt
     1921 cgggatggaa ctggcatccc agaaaccagg gatgatcttt gatttccaca atttccgcca
     1981 aacgtccgtc gggtgaaatc cctgttatgc gcaatccctg attcatgagc gcttctttgt
     2041 aatcgttatt gaattcgtaa cgatgacggt gccgctccga aatttcaccc tgaccgtaag
     2101 ctttacaggc gaaggagtcc gcctcgacaa ggcagggcca cccgcccaat ctcatggagg
     2161 cgcccttttc tttgacatgg cgttggtcgg ggagcagatc aatgaccgga taggacgtct
     2221 ccggatcgaa ttcggaactg ttggccttct ccagtttgca aacgtttctg gcatattcga
     2281 cgacagccat ttgcatgccc agacaaatac cgaagaaggg aatctttttt tctctggcgc
     2341 gctgaatggc gagaatcatg ccctcaatac cgcggatacc gaagccgccg ggaaccagaa
     2401 cggcgtcgat atcgtccaga tgtttgccca ggccttccgc ttcaattttt tccgaatcga
     2461 taaatttcag tttcacgcgg cagttgttgg caatgccccc atgcacgagc gcctcgttga
     2521 ggcttttgta ggaatccgtc agattcacgt atttgccgac gatgccgatc gtcacgtcat
     2581 gcaaagggtt gtggaactta tcgaccacat cctcccaaac gtcgagtttc ggctgtcctg
     2641 tccagatgtt gagaagatcg acaatcttcg catccagtcc ctcccggtga aaaatcagcg
     2701 gcacttcgta gatgcagctg acatccttgg cggtaaagac agaatggacc tccacgttac
     2761 agaaaagagc aatcttggat ttgatatcat cggaaaggaa attttccgtc cggcaaagca
     2821 ggatatccgg ctggatgcct atttctctga gcgccttgac gctgtgctga gtgggcttcg
     2881 tttttacttc accggctgtc ttgataaggg gcacccaggt taaatgaatg aaaatggcat
     2941 tttctctgcc gacttcattg cggaactgcc ggatggcttc caggaaggga aggctttcaa
     3001 tatccccgac ggtgccgccg atttccacaa tggccacatc aaaaccggct gctgtgtcgc
     3061 ggatgaaatc ttttatttcg ttggtaatat ggggaatgac ctgaacggtt tttccaagat
     3121 aatctccgcg ccgttcttta gtgatgacgg cgtagtaaac ctgtcctgtg gtcaggttgt
     3181 tgcttttgcc catgcaggtg gagcagaatc gttcgtaatg tcccagatcg agatcggttt
     3241 ccgcgccatc atcggttacg aacacttcgc cgtgctgaaa agggctcatg gttccgggat
     3301 cgacattaat ataagggtcc agtttttgat tggtgacttt cagaccccgg ctctccagca
     3361 gggcggagat ggacgcggct gccagacctt ttccaagtga agaaagaacg ccgccagtga
     3421 tgaagatgaa ttttgtatgt ttcacaatgc cccttcctga tgtgtttatg cgaaggttaa
     3481 atgtccgatg gctaagccaa gttttttaaa ataacccctc ttgttttaag ggcgcttgta
     3541 ttttccaaaa ggccctgggc agcgatacga tcaagaacat agatcaactc gccgcctttt
     3601 tgggaataag cctgaccgta cgatatgggg atttctggcg tcatgtttcc caggattgac
     3661 cgcgtaacgg attccgtttt gcgtgcgccg ttggcgacca gaacgacggt ttttgccttg
     3721 taaaccagtt cagcccccat gctgattgca tattgcggcg aatccgcctt ggttttaaag
     3781 tgcccgtcgg ccaccgcgtt ggcgatggtg ttgtcgtcca gtttgaccag cagcatggaa
     3841 ctgccttgaa aaggaatgcc cgattcgtga aaggccacat ggccgcgacc gcccacaccg
     3901 ataatctgca gatcaattcc tccaaccgct tttatttttc tgacgtaacc gtcgagaatg
     3961 tctttccgga tccaggcaag ataaggtgat ttcgcttttg ccttgatcgc aatggatttc
     4021 ccggcgtccg ttcccttgaa ttcccagtcg cttttattgt ccttcaattc ccggatgagc
     4081 attttctggt cgatcaggga accataagga acacgggttt ctataaattt tttattgaga
     4141 tgaccgaaaa gttcctgaat cataaagtag gcatagcttt ccggatgcat aacgcgctgc
     4201 tgaatattgt tccccggtaa gccgacatat tcatcgagat taaaactgcg aatgcggccg
     4261 gcatccaatc ggccttcatt gaccgctttg gccaaatgct tataaaggcc ggtcggtgaa
     4321 tttcccgtgg ccaatcccag agcgatctct tttttccttt taagcagttc gatggatttt
     4381 ttaataacga tgcctgaagc aacgtcactc atgtgcgcga agtctttggt tacccaaatc
     4441 gttgttccca ttctgtttat ccttccccct tatccgattt cgcttttgat gatatccgca
     4501 agctgccggc aatatttcag cgtgacggca tcggtcggac cttcgaccat cacgcggcac
     4561 atgttctgcg ttccggaata cctgaccagg acacgccctt cgtcacccag ctctttttcc
     4621 acctgtttga tggcttccgc cagcctggga atttgatcga tatccggctt gcttttaaca
     4681 tcaacattga tcaacttttg cggaaaaatg tccatcatgc gcgccagttc ggaaagaggc
     4741 ttgttttctt taatcatggc ggctactagc tgcagggctg caataattcc gtcgcctgtg
     4801 gtgtgatgat ccagaaaaat catgtgtccg gcttcttctc cgccgatcac ggatcccatc
     4861 ttcagcatat cttcaaggac ataacggtct cccactttag acgcatgatg cttgaatccg
     4921 tattgcttgc aggcgacctt cagacccaga ttgctcatga cggtgctgac cagaagatcg
     4981 ttttttaatc gtccttcccg ctttaagacg ttggcgcaga tgaccagaat ctgatctccc
     5041 gttatttcct ggcctttttc atccacggca atcaggcgat cgccgtcccc gtcaaacgcc
     5101 aagccgatgg ccgctccgtt ttccagcacg gtctttttca gacgttccgt gtgctgggat
     5161 ccgcactggt cgttgatatt caaaccgttc ggcgtcttgt gaatgacgtc gatatccgcg
     5221 cccagttcat ggaacacatc cggcgcaacc cgataggtgg ccccgttggc ggtgtccaga
     5281 acaatcttca tgccttccat cgacagattg cggggaaacg tgttttttac aaaaacgatg
     5341 tagcgtccgt tgacgccttc caggcggaag gcccggccga tatcgtcgcc tttggaaagg
     5401 aattccgaaa gcttgtcgtt taggatcaga tcttcgatga tgtcttcttt ttcgtcggtc
     5461 agtttatagc cgtctcctcc gaatattttc agtccattgt cctgaaaggg attatgggag
     5521 gcggaaatga ccacacccgc atcggcccgc atactctgtg tgacaaaggc gattcccggt
     5581 gtcggcatga cgcccaccag ataggaatac cctcccatcg aggtaatacc tgcctccagg
     5641 gagctttcca gcatataccc cgaaagcctg gtgtctttcc cgatgatgat aacagaacgg
     5701 tgtcctccct tcttaaatac atgcgcgata gcctgtccca ctccaaaagc aatttgcggt
     5761 gtcatgggat gccgattagc ttctccacgt atcccgtctg tcccgaataa tttacccatt
     5821 gttctactcc ttatctttag atatctcgcg tcgaacaaat tgattgttaa aatgttttta
     5881 ataaacccag cttgggtacg gctctggata cgttttcacc aagatctcgt acgatgccgt
     5941 ccagccacat gacgcctttc gatgaaattc cggctggcag cgcctggatt gtttcaatat
     6001 aatttgtttt gtttttcccc gcaatgcttt ccagatcgtt gagaaacgtg tcacgcagct
     6061 cgtcggtttt tcccgaggcg ggggacgctt tcccgttgca gaggtcttca atatgtccca
     6121 tcttttcatg aagctctttt ctgccccgcg cacaggttcg ttcaggattt ttttcttctt
     6181 ctgctgcggc ggcggctttt tgggccatag cctttaatcg ttttaatcgt tcctttcttg
     6241 ctgaagaaag ttgctccatc accccggcgt ataaaaggga accgtattcg ccggtttcca
     6301 gaaaaggttt cctcacatgc gtgtaccagg cttcaagcgc tttcaaattt gcaaggtaga
     6361 ggatattgtt ttccacaatc cgttccaggt ggggataggc cgccgggtaa aacggtttca
     6421 cactgcaggc cggcgctttc tcaaagatga gttgattgtc tccggggtag tcgtgccgca
     6481 ggacggaacc ggcggcgacg acatttccga atccaagtct caccggaccg atgattccgc
     6541 cctggccacc cagaaaaatc ggcggttgat tgagcatgac gccccgcgga acgtcgccca
     6601 gcagagaagg cgttgtttta tcggcatccg gtgtaaaatt aaaatgaatg taggaactgc
     6661 ccacttctga atgattgctt cggctggtgc cgccgcccat caggcaatcg cagaagttga
     6721 tcagactgcc cagcgttacg aacgggaaaa gaatggtctg cttcaagccg acacaatgcg
     6781 caccaccggc ttcttcttcc agaatgcagc cttcccggac gtgcgcgccc attcccatat
     6841 ttgatttttc cagaaagacg gatttgctga agaaccctcc ctttaattca accttttcgg
     6901 ccagttgaca gttttcaatg gtaacgggcg cttcgtatcc cagttgacag ccggatgata
     6961 tggtcgtttc ctttccgtag atccggcaac cgggatatat cttgaccccc ttacccgaaa
     7021 tccggtcgat agcgacgtca tcgccgatat cgatggtttc gggattggga atgctcacac
     7081 cgcgttcgag cagatcattt atttttttga gcgatctcgg ttttaaatcc atacggtctc
     7141 cctgacgtta aggacggaag atgcttcagc attcgcgttc gttaaaccac gccgtaagcc
     7201 agcatggcat tggcgacttt ggtaaatccg gcaatattgg ccccggtcac atagtcgccc
     7261 ttcttcccgt aggtttctgc cgtgtcgtag caatttttgt gaatgctcct cataatgatg
     7321 agcaggcggt tgtcaacttc ttcacgcgtc cataacagcc tgagactgtt ctggctcatt
     7381 tccaggccgg acgtggcgac gccgcccgca ttggccgctt ttccgggacc gtaaaggatt
     7441 tttttatcct ggaaaatttt gatggcttca ggcgttgagg gcatgttggc tccttcggca
     7501 acacagagac agccgttttt caccagtgcc gccgcgttct taccgttgat ttcattttgc
     7561 gtggcgcagg gcagggcgat gtccactttg atgccctgtt cgcgaatgac gtcccagaca
     7621 tttttgcctt cgaagcattc cgatttgaat ttgtttgcgt attcggaaat gcgcccgcgt
     7681 ttgacgtttt tcagatcaag cacgaagctg catttcttgt catcaatgcc ggcttcatca
     7741 ataatacacg acgaagaatc acacagagag atgaccttgc cgcccagttg gttgactttt
     7801 tcaaccgcat actgagccac gttgccggcg ccgctgacgg cgacggtttt tcctttgaaa
     7861 tccattttgc gggtggccag catttccgcg gcaaaataca cacagccgta acccgtcgct
     7921 tcgggacgaa tcagactccc gccgtatgac attcctttgc cggtcagcac gccggtgtgc
     7981 tcgttgcgta tttttttgta atagccgtac ataaagccga tttcacgacc gccgactccg
     8041 atatctcctg cggggacgtc cacgtcggcg ccgatgtgac ggtacaattc gcgcataaag
     8101 gcctgacaga aacgcatgac ttctccgtcg gattttcctt tggggtcgaa atcggaaccg
     8161 cctttggcgc cgcccatggg cagggtggtc agtgcatttt tgaaaatctg ttcaaacccc
     8221 agaaatttga tgatgcccag attcacggac ggatgaaaac ggataccgcc tttgaagggg
     8281 ccgatggcgt tgttgaattg cacgcggaat ccgcgattga cctgaacgtt tcccttgtcg
     8341 tccacccagg gaacccggaa aatgaccgct ctttccggct cgacaatcct ttcgtaaagg
     8401 ttggctttga cgaactcggg atgctttttg acggttggtt ccagcgtctc cataacttct
     8461 tcaacagcct ggtgaaattc cggctgattg ggatcagtct gcttgacttt ggcgatcaca
     8521 tcttgaataa ctgacataaa ttatcatcct cctaaaaaat tgttattgat ttcatttttt
     8581 gacgttcatg atgcaacgtt tttcatatat cttcaacccg ctgaagcgtt tcatactcca
     8641 tgacggtgga catcgcttta ttttctatgt cgaacccttc ttcgccggct gcggcaatcg
     8701 ggttgagtcc tccaagaaga atcatgccca ctttgttgat atccacattc gtctggcaaa
     8761 ccggatttcc aacattaccg atcgaaaggg cgccgttaac gccgatcttt ttaaaatcag
     8821 caacgatgtt ttccaccaga gagagcgcca gtgccgggat ttctcgaaaa tttgccagaa
     8881 tctttccctc cccctttttg acaacgtctt tgacggaggt cattttgccg cggataaaaa
     8941 tttcggacgg atcaagcgac gagccggaat aattgatgag ctccacgaaa cgcaaaggat
     9001 taccgtgccg cacctgcaag atgccgccga atttggaatc catcggaatg ccgtgcttca
     9061 gaagaacgcc gttaatgaga atgctgcaaa cggtggcaaa tccgattttc ccctcgggaa
     9121 tggtgacttc tcccagcgtt tcaccggcag acgcaacagc tacaagatcc gacacggcaa
     9181 ttttcttttt gaaaaccggt ttcatgacgg ccaaggcctt gcggaattgt ttttgaggga
     9241 acagagaaat attaacggga acaaggccgc gccgtttttt tatgtcgaaa gtcgttttta
     9301 aggaaagaac atcaatccgg gaaatggaaa gactgatttt gtcccggacg cgggcattgt
     9361 tgatttcttc catccccatg tctgtgatga ttctgccgtc acgccgcccg atgagcctgg
     9421 tcaagcctct ttcatccatc gacttcaagt gatagcggac agttctttcg cttaaatgaa
     9481 cgccgtgttc tttcattttt cgcgcgatga cgcgggcgcc tgcggattct gaatttttgt
     9541 tgagaatctt gagaatcaga aggacttttc tttctatgtc ttcgggattg aataaatgtt
     9601 tcatagctaa tcggcaaccg tttgccgtat atctactttc aaatgatgtc cttgtcaata
     9661 aaaaataata tttttttaaa cggattttca caggaa
//
LOCUS       DAONQZ010000078         9521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_7838_length_9521_cov_18.530319, whole genome shotgun sequence.
ACCESSION   DAONQZ010000078 DAONQZ010000000
VERSION     DAONQZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_9521_cov_18.530319"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2879)
                     /locus_tag="PLR13_10025"
     CDS             complement(150..2879)
                     /locus_tag="PLR13_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQC19718.1"
                     /translation="MPQSIVQYDKTLPYIEDRNPWEVPVSYLKRKTANEYELVPGRRP
                     SKMLLVNKLRSEIDKWRNNGYPGATVTSRELLTFWFDQSHPPVFGNEPFHFYFCQREA
                     IETIIYLFEIKKFTDVIPLIQKYHEKFSGNLFASTVEFSEDLEGKRKVKRYFPEKDQE
                     GEQELPEKDLLRYAVKMATGSGKTFVMTFVVVWSYFNRLREKDRRYANNFLLVAPNVI
                     VYERLAKDFVNGKVFMEFPFIPEIWKSQWKLNIKLRGDESPLAAENNFILNNIHQLYE
                     SRTKEFNPNTIIEAILGRKPQSDPGKSPQTLLERIKELDNLLVMNDEAHHVHDDELKW
                     NESLLVIHKNIPGGLKMWVDFSATPKTPENTYYPWIICDYPLAQAVEDRIVKVPLIVH
                     SVNKKDPERITKDNVVQKYGDWISAALARWKEHNEAYKGVKKKPVLFIMAEKNVFADR
                     IAESIARRGTTYGINRDEIMVIHTDNSGDVRNSDLERLREEARDVDNPKNKIKVIVSV
                     LMLREGWDVQNVTIVLGLRAFDSAILPEQAIGRGLRLMRDISPDRTQTLEVIGNDNFE
                     KIVKQLELEGVGINTTNTPPPLPVTIAPEKKRLPYDIEIPATDFSYTRAYKNLSGING
                     KIFPSLFSSSKLSEDRKTLLKMEVMNLGVEVHQIAVESEFLELGRDLTSYIVSQVMKK
                     AGLTDCFQFVYPICEDYILNKCFEVKIDDIEKEELRKVLRELSIQEAIIELLATEIGK
                     ATVEKKQIKVKGKNIKLSDTPKFTWRRGHIRLKKTVFNFVAIYNPFEAEFAEFLDNAS
                     DIEAFAALADIFRIDYLSNSGAIRYYFPDFVAVQLVGKKKVFWILETKGREYPDLDNK
                     NKAIERWCKAVSEQTGYEWKYLLVKQVIFNVDKKSTKTLKQLLDKINSQAKIE"
     gene            complement(2882..>3751)
                     /locus_tag="PLR13_10030"
     CDS             complement(2882..>3751)
                     /locus_tag="PLR13_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19719.1"
                     /translation="WIGVDLGRYAIHTTRKRLLEIEHSKSLTEEGKKYGKKARPFEIL
                     NLGKYERQFWQVKAFGRKDEKQALYEYLAFILKLYGAEPIAGSSHIHGKKGNALVYIG
                     AVDSPVTIQEISDALKDTKDMGQKELHVLGWEWEMGLNDAIQEVARQMAVRLKLRIIP
                     MDAIDPQAAGKDDVKFFELAYFKTEIETKALKVKVKLEDFVIPHTDLIPPDVKESIKK
                     WSDWIDYWAVDFEFQNDTFNNGWTSYRTKQERKLRLVADHEYSKPGTYKIFVKIIDIF
                     GIDTSQVYEIKVK"
     assembly_gap    3752..3851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3852..4096)
                     /locus_tag="PLR13_10035"
     CDS             complement(<3852..4096)
                     /locus_tag="PLR13_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19720.1"
                     /translation="MSKIDIKKTELVWRGKYDDEGKLVPVEKPGPFPFQIVESINDPR
                     VEKGDEVPKQTTLFDTWKGDEGNTFEDGWKNKLIWGD"
     gene            complement(4101..5588)
                     /locus_tag="PLR13_10040"
     CDS             complement(4101..5588)
                     /locus_tag="PLR13_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HQC19721.1"
                     /translation="MSRLKIFISSVQSEFASERQMLFNYITSDPLLGQFFEVFLFENL
                     PAKDKSATEAYLDEVRQSDIYLGIFGKEYGFEDREGISPTEHEYDLASSERKTRLIFI
                     SNHKTEERHPKEHSLIKKAENAVVRKMFSDASELKTAIYSALITLLKEKEFIRTGPFD
                     ASICKEAVINDIDNERIKWFVEAARRKRGFPLSSDEHIEKILTHLHLIKNGKLTNAAI
                     LLFGKNPQRFFISSEIRCALFHGNEIQKPIPSYQVYKGDVFQLVDQAIDFVLSRIDVY
                     VGDRSQSVDVDVKYEIPRMAVTEAIVNAVAHRDYTSTGSVQVMIFRNRIEIRNPGRLP
                     HQLTLADLKKDHESFPANPLIAEPLFYAGYIERLGTGIPDMVKNCIGAGLKEPEFMQE
                     TSFRAILWRKTRVVDQVTDQVTDQVTDQVQRLIIVMNKELTRQELMQILEMKHNQTFR
                     ENYINPALGRQLIEMTIPDNRNDPNQKYRLTAKGKQLQKQLKEKK"
     gene            complement(5585..8413)
                     /locus_tag="PLR13_10045"
     CDS             complement(5585..8413)
                     /locus_tag="PLR13_10045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQC19722.1"
                     /translation="MTELQTKFIETFGLDSDKEKQKAVFETDGPTLIIAGPGTGKTYT
                     LVLRTLYLILSGKAKPSEIVLTTFTEKSAFELRDRLSLFSKRLGEKTNLHELITGTIH
                     GICDTFNNTFIKQTPLKKNYTVLDDLTSLLFINEHFETIIEPFHDGDKYFGRWKGKWD
                     TIDRVKKFYDKITEEIIDPDDLIASSDSFLQQLGESYKSYRNLMFENNRVDFSFQQRI
                     FYDLLQNKSTREKIASTIKYMLIDEYQDTNYIQEQIALTLVKPHNNITVVGDEDQALY
                     RFRGATVRNILEFETHFKKCRKIKLLENFRSHVTIIEHYNAFMQQIDWTNPKGNVPFR
                     YPDKEVIPAKSTISPDYPAVFCIWTDTPRAEAERFADMVEYLLKNKIINDLSDVALLL
                     RSVKLEYSEPFITALNNKNIKAFCPRAKAYFENEEIKLLIACYALIFEFVGGDLDNYP
                     HKEIINEGIQLLGKYVSRPLGAYIRLMNEKINALKDKESLDELPSDIPYQLFAYAPFS
                     DYLKDENAARNLSQFSSLINSFMTYYHIPLISAKNKMALKYRLFNSFFNLLFNTGQNE
                     YEDENSPIPKGYVQIMTIHQSKGLEFPVVVVSSLDKQYSVNKQINRFLSPFYTRAVFE
                     PEDRITQFDHARLFYVAFSRPQKILVLSTSGEPKDIFDSIWEGLDQWPHVKKETLKAQ
                     RFHSKDPYVPKKSLSLTSHINIYETCPRQYLFYKEYEFTPSRSGQLLFGTLVHQTIED
                     LHRRILDGEKANEKIISEDFENNYKGLLANGYRPLGILPKQGALRHCLNYFQNNKDLL
                     NRVIETEIDVSYEKDDYIINGKVDLLLGKDEKLEVLDFKSQPRPVKNDTVLEKYRYQL
                     HVYAHILKERYQKDPERLYIYWTAEDDRKDALMEINYDQKLVEAAGKHFDSVAKCIIK
                     KDFEIKTKPDKTKICKECDFKHFCRITV"
     gene            complement(8417..9112)
                     /gene="radC"
                     /locus_tag="PLR13_10050"
     CDS             complement(8417..9112)
                     /gene="radC"
                     /locus_tag="PLR13_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQC19723.1"
                     /translation="MKVKGHIDKPHYLDHRSRLRNRFLKQGISGLQPYEIIELFLTFV
                     VPQKDVKPAAKEAINKFGSIKGFFDADEQELSQIPFFKEKALALRKFIKEISLLYYKQ
                     QAEQLPVTQSKEDLINYCKAKLGFLPNEEFWMISLDSKKAIVKEDLISKGLTDKTPVY
                     PKQVIEKAMKNGASSILLLHNHPNGNPQASQQDITITKAIDIPARVLNISIYDHIIVA
                     QDKHFSFKENKLV"
BASE COUNT         2551 a         1912 c         1722 g         3236 t
ORIGIN      
        1 tgattcttct catctgacta acgaagaatt cacaacaaaa gtttcaataa ccaaatcatt
       61 tttggggcgg gaagtgttat ttctgtctgg ttttgaaata gttagcggtg gctagcggag
      121 cttttaaatg tgcttgcaga tgtattgact tattcaattt ttgcctgact attaatttta
      181 tctaataact gctttaaagt tttcgttgat tttttatcca cattgaatat tacttgttta
      241 actaacaaat atttccattc gtaacctgtc tgttctgata ctgctttgca ccagcgctct
      301 atcgctttgt tcttgttgtc aagatcgggg tattcacgac ctttagtttc taaaatccag
      361 aacacctttt tcttaccaac taattgtact gccacaaaat cgggaaaata ataacggatt
      421 gctccggaat ttgaaagata atctattctg aatatgtctg ccaacgcagc aaaagcttca
      481 atgtcgcttg cattatcaag gaattctgca aattcagctt caaacgggtt gtaaattgca
      541 acgaaattaa aaacggtttt ctttagccgt atgtgacccc gcctccaagt gaatttaggt
      601 gtgtctgata gttttatgtt ttttcctttg acttttatct gttttttctc aactgtagct
      661 ttccctattt ctgtcgccaa tagttcgatg atagcctcct gaatggagag ttctcgcaga
      721 acttttctta attcttcttt ttcgatatca tcaattttaa cttcaaaaca tttatttaga
      781 atatagtctt cacaaatcgg ataaacaaac tgaaagcaat ctgtcaagcc ggctttcttc
      841 atcacctgcg aaacaatgta agaagtaagg tctctgccta attcgagaaa ctcagattca
      901 acggctatct ggtgaacttc aacacctagg ttcatcactt ccatttttaa aagcgttttc
      961 ctgtcttcgc ttaattttga agatgaaaac aacgatggaa atattttgcc gttaattcct
     1021 gaaaggttct tatatgccct ggtatatgaa aagtcagttg ccggaatttc aatgtcataa
     1081 ggtagtctct ttttctcagg agcaatagtt acaggcaatg gcggtggtgt gtttgttgta
     1141 ttaatgccta ctccttcgag ttccaattgc tttacaatct tctcaaagtt gtcatttcca
     1201 attacttcca gcgtttgggt gcggtcagga ctgatatctc tcattaaacg caaacctctg
     1261 ccaattgcct gttccgggag tatcgcagaa tcaaaagcac gcaaacccaa aacaatggtt
     1321 acattttgaa catcccaacc ttcacgaagc atcagaacac taacaataac cttgattttg
     1381 ttttttggat tatcgacatc acgtgcttct tcccgtaatc tttccaaatc actattccgg
     1441 acatcacctg aattgtctgt gtgaataacc atgatctcat ccctatttat gccataggtt
     1501 gttcctcttc tggctattga ttcagcaatt ctatcagcaa agacattctt ttcagccata
     1561 ataaaaagca caggcttctt tttcacgccc ttgtaggctt cgttatgttc tttccagcgg
     1621 gcaagagcag ctgaaatcca gtcgccatat ttctgaacca cattgtcttt tgtgattctt
     1681 tcagggtctt tcttgtttac agagtgaact attaaaggaa ctttcacaat gcggtcttcc
     1741 actgcctgtg ccaagggata atcacaaata atccaggggt agtaagtgtt ttcgggtgtt
     1801 ttaggcgttg cagaaaaatc aacccacatc tttaatccgc ccggtatgtt tttatgaatg
     1861 acaagcaaac tttcattcca ttttaattca tcatcatgaa cgtgatgcgc ttcatcgttc
     1921 atcaccaata aattgtcaag ttcctttatc ctttccagta aggtttgagg agatttgccc
     1981 gggtcactct gtggttttcg gccaagaatg gcttctatga ttgtgttcgg gttaaattct
     2041 ttagtgcggc tttcgtaaag ctgatgtata ttgtttagaa tgaagttgtt ttcagcagcc
     2101 agaggacttt catctccacg aagtttaatg ttaagtttcc attgcgattt ccatatttcg
     2161 ggaatgaaag gaaattccat aaaaacctta ccattgacaa agtcttttgc cagcctttca
     2221 taaacgataa cattaggtgc aaccagtaaa aagttgttgg catatcgtct gtctttttct
     2281 cttaagcgat tgaaataact ccaaaccact acaaacgtca tcacaaaggt tttacctgaa
     2341 cctgttgcca ttttgactgc ataccgcaac aaatcttttt ccggcaactc ttgctcgcct
     2401 tcctggtcct tttcagggaa atatcttttt acttttcgct taccttccaa atcttcactg
     2461 aattcaacgg ttgaagcaaa caggtttcca ctgaatttct catgatattt ctgaattagc
     2521 gggattacat cagtgaattt ctttatttca aaaagataaa tgatggtttc gatagcctca
     2581 cgctggcaga aatagaaatg aaacggctca ttgccaaata ccggagggtg cgactggtcg
     2641 aaccaaaatg tcagcaattc ccgtgaagtt actgtagctc cgggataacc attatttctc
     2701 catttatcaa tttccgaccg gagtttattt accagaagca tcttactcgg cctgcggccc
     2761 ggaaccaact catattcgtt ggctgtcttc cttttcagat aacttaccgg cacttcccac
     2821 ggattgcggt cctcgatgta aggaagcgtt ttatcgtatt gaactatgga ttgtggcatg
     2881 gctattttac tttaatttca taaacctgag aagtgtcaat tccgaaaata tcaatgatct
     2941 tcacaaaaat cttgtatgtt cccggtttgg agtattcatg gtcagccacc agccggagtt
     3001 tgcgttcctg tttggtacga taactggtcc atccgttgtt aaaggtgtcg ttttgaaact
     3061 caaaatctac agcccagtaa tcaatccagt cgctccattt tttgatgctt tctttcacat
     3121 cgggtggaat tagatcggta tgcggaatta caaaatcctc tagttttact tttactttca
     3181 gcgctttggt ttcgatctcg gttttaaaat atgccagttc aaaaaatttc acatcgtctt
     3241 tgccggctgc ctgcggatca atggcatcca tgggaatgat tcggagtttg agccgcactg
     3301 ccatttgcct ggcaacttcc tgtatggcat cgtttaaacc catttcccat tcccaaccta
     3361 atacatgcag ctctttttga cccatgtctt tggtatcttt tagcgcatca ctaatttcct
     3421 gtatggtaac cggggaatca acagcaccaa tatacaccag tgcattgcct tttttaccat
     3481 gtatatgtga tgaaccggca ataggctcag ccccatatag cttaagtatg aaagccaagt
     3541 actcgtataa tgcctgtttt tcatcttttc tgccaaatgc tttaacctgc cagaactgcc
     3601 tttcatattt gcccaggttt agaatttcaa aaggacgtgc ctttttacca tattttttac
     3661 cttcttctgt taaactttta ctgtgctcaa tttccagcag acgcttgcgg gtggtatgaa
     3721 tagcatacct gcccaaatcc acacctatcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nttgtctccc caaatgagtt tgtttttcca gccgtcttca aaggtgtttc
     3901 cctcatctcc tttccatgta tcaaacagag tggtttgctt tggaacttcg tcgccttttt
     3961 caacccgcgg gtcgttaatg ctttccacta tctgaaaagg aaagggacca ggtttttcaa
     4021 ccggcactaa cttgccttca tcatcatatt taccacgcca aacgagttcg gtttttttga
     4081 tatcgatttt gctcatgata ttattttttc tcttttaatt gtttctgtaa ttgtttgccc
     4141 tttgccgtga ggcggtattt ctggttcggg tcattgcgat tgtcagggat tgtcatttca
     4201 attaactgcc gcccaagtgc cggattaata taattttccc ggaatgtttg attgtgcttc
     4261 atttcgagta tttgcattaa ttcctgtctt gtgagctctt tattcatcac gataatcagt
     4321 ctctgaactt ggtcggttac ttggtcggtt acttggtcgg ttacttggtc aacaaccctt
     4381 gttttcctcc atagaatagc cctaaaggag gtttcctgca taaactccgg ttctttcaac
     4441 cctgctccga tacagttttt caccatatct ggaataccgg tgcccaggcg ctctatgtag
     4501 ccggcataaa aaagcggttc ggctataagc ggattggctg ggaaggattc atggtctttc
     4561 ttcaggtcag ccagtgttag ttgatgaggc aggcgtccgg gattccgaat ttctatccgg
     4621 tttctgaaaa tcatcacctg cactgagccc gtgctggtat aatcacggtg ggcaacggca
     4681 tttacaatag cttcggttac cgccatccgc ggaatttcat atttcacgtc tacatccaca
     4741 gactgacttc tatcacctac atacacatca atacgtgaaa gcacaaaatc aatcgcctga
     4801 tccaccagtt gaaacacgtc tcctttatac acttggtagg atggtatggg tttctgtatt
     4861 tcattaccgt gaaaaagggc acatcgtatt tctgaagaaa taaagaatcg ttgtggattt
     4921 tttccaaata gcaggatggc cgcattggta agttttccgt tcttaatcaa atgtaaatgc
     4981 gtaagaatct tttcaatatg ttcatctgac gacaaaggaa atcctctttt tcttcgtgca
     5041 gcttccacaa accattttat acgctcgtta tcaatatcgt taattacagc ttctttgcaa
     5101 atggaggcat caaacggacc tgtacgtatg aattcttttt ctttcagtaa tgtaataagc
     5161 gcagaatata tggctgtttt aagctcactg gcgtcagaaa acatttttct gaccacagca
     5221 ttttcagcct tttttataag actatgttct ttaggatgcc gttcttcggt tttatggttg
     5281 ctgataaaaa tcagccgggt tttgcgttca ctgcttgcga ggtcatattc atgttcagtc
     5341 ggagaaatcc cttccctgtc ttcaaaacca tattctttcc cgaaaatgcc aagatagata
     5401 tctgattgcc ggacctcatc aaggtatgct tccgttgcgc ttttatcttt tgcaggcagg
     5461 ttctcaaata aaaaaacctc aaaaaactgt cccagcaagg ggtcggatgt aatataatta
     5521 aaaagcattt gcctttctga tgcaaattct gactgcacac tgctgataaa tattttgagt
     5581 ctgctcatac tgtaatccgg caaaaatgtt taaaatcaca ttctttacaa atctttgttt
     5641 tatctggttt ggttttaatc tcaaaatctt ttttaataat acattttgca acagaatcaa
     5701 aatgtttccc ggctgcctcg actaattttt gatcatagtt tatttccatc agggcatcct
     5761 tgcgatcatc ttcggcagtc cagtaaatgt acaatctttc ggggtctttc tgatagcgtt
     5821 cttttagaat atgtgcgtac acatgaagct ggtagcgata tttttccaaa acagtgtcat
     5881 tcttcacagg gcgaggctgg cttttaaaat ccaacacttc tagcttttca tctttcccta
     5941 ataaaaggtc aaccttgcca ttaataatgt agtcgtcttt ttcataagac acatcaattt
     6001 cggtttcaat tactctgtta agtaaatctt tattgttctg gaaataattc aggcaatgcc
     6061 gcaatgcccc ttgttttggc aagataccaa gagggcgata accattggca agaagtcctt
     6121 tataattatt ttcaaaatct tcggaaatta ttttctcatt cgctttttca ccatccagaa
     6181 ttctccgatg aaggtcttcg atagtttgat gcactaatgt tccgaacaac aactgcccgg
     6241 aacggctcgg tgtaaattca tattccttgt aaaacaaata ttgcctagga caggtttcgt
     6301 aaatgttgat atgtgaagtg agtgataatg attttttagg aacataggga tcttttgaat
     6361 ggaaacgctg ggctttcagg gtttcctttt taacatgagg ccattggtca agcccttccc
     6421 atattgaatc aaaaatatct ttcggttcac cgctggtcga cagcaccaat attttttgag
     6481 gtcttgagaa ggcaacataa aacaggcgtg catggtcaaa ttgagtaata cgatcttccg
     6541 gttcaaatac ggctcttgta taaaagggcg ataaaaaacg attgatttgc ttattcacgc
     6601 tatactgctt atctaaagaa ctaaccacta ccactggaaa ttcaagtccc tttgactgat
     6661 gaatagtcat gatttgaaca taaccttttg gtatggggct gttctcatct tcatactcat
     6721 tttgtccggt attaaaaagc aaattgaaaa agctattgaa aagacggtat ttaagagcca
     6781 tcttattttt tgcactgatg agcggaatat gataatacgt catgaatgaa ttgataagag
     6841 aagaaaactg cgatagattt cgagcagcat tttcatcttt cagataatcg ctgaatggag
     6901 cataagcaaa cagttgataa gggatatcgg atgggagttc gtcaaggctt tctttgtctt
     6961 tcaaggcatt tattttttca ttcatcagcc ggatatatgc tcccaaaggt cttgaaacat
     7021 attttcccag aagctgtatt ccttcgttta taatttcctt gtgtgggtaa ttatcaaggt
     7081 ctcccccgac aaattcaaat atcaatgcat aacaggcaat taaaagttta atctcttcat
     7141 tttcaaaata tgctttagcc ctcggacaaa atgcttttat gtttttattg tttagcgcgg
     7201 taatgaatgg ctcgctgtat tctaatttta cacttcgcaa taacaaagca acatcactga
     7261 ggtcgtttat gattttgttt ttaagcagat attccaccat atcggcaaaa cgttcggctt
     7321 cggctctggg tgtatctgtc caaatacaaa acacagccgg ataatctggt gaaattgtgg
     7381 attttgcagg gataacttct ttatcagggt atctaaacgg aacattccct ttcggatttg
     7441 tccagtcaat ctgctgcatg aatgcattgt aatgttcaat aatggttaca tgagagcgaa
     7501 agttttcaag caattttatt ttcctgcatt ttttaaagtg cgtttcaaat tcaagaatat
     7561 tcctcaccgt agcgcctcta aaacgatata atgcctggtc ctcgtctcct acaaccgtaa
     7621 tgttgttatg aggtttgacc aaagttaaag ctatctgttc ctgtatgtag ttcgtgtcct
     7681 gatactcgtc gataagcatg tatttaatgg tgctggcaat tttttctctg gtacttttat
     7741 tttgtaggag gtcatagaaa atcctttgtt gaaaagaaaa atcaacacga ttattttcaa
     7801 acatcaggtt acgataactt ttgtatgatt ctccaagttg ttgcaggaaa ctatctgagg
     7861 aggctattaa atcatcagga tcaataatct cttcagtaat cttatcatag aattttttga
     7921 cgcgatctat tgtatcccat ttacctttcc atctaccgaa atatttatct ccatcatgaa
     7981 aaggttcaat tatagtttca aaatgctcat taataaaaag gaggctagta agatcgtcaa
     8041 gtacggtata atttttcttg agaggtgttt gtttaataaa agtattgttg aaagtgtcgc
     8101 atattccatg aatagtgcct gtaattaact catgcagatt agttttttcg cctaatcttt
     8161 tcgaaaataa acttaaacgg tctcgcaatt caaaagctga tttttcagta aaagtagtca
     8221 gcacaatctc ggatggtttt gccttgccgg aaagaatcaa atacaaagtc ctcaatacaa
     8281 gagtatatgt ttttccagtg ccgggaccgg caataattaa tgtcggacca tcagtttcaa
     8341 aaactgcttt ttgcttctct ttgtcagagt caagtccaaa agtttcgata aatttcgttt
     8401 gcagttctgt cattctttaa acgagcttat tttctttaaa actgaaatgc ttatcttgag
     8461 ccacaataat atggtcataa atggaaatat tcagaactct tgcaggaatg tcaattgctt
     8521 ttgtaattgt aatatcctgt tgtgatgcct gcggatttcc gtttggatga ttatgcagga
     8581 gtaaaattga actggctccg tttttcattg ctttttcaat cacttgtttt ggataaacag
     8641 gagttttatc ggtaagccct ttcgaaatca aatcttcttt gactattgct tttttgctat
     8701 caagcgaaat catccagaat tcctcgtttg gcagaaatcc aagttttgct ttacagtagt
     8761 tgattaggtc ttcttttgac tgagtgaccg gtaactgctc tgcttgctgc ttataataga
     8821 gcagagaaat ctctttaata aatttacgaa gtgcaagagc tttttctttg aaaaaaggaa
     8881 tctggcttag ttcttgctca tcagcgtcaa aaaaaccttt aatactaccg aatttattga
     8941 tcgcttcttt tgctgccggt ttgacatctt tttgagggac tacaaatgtg aggaataact
     9001 caattatctc gtaaggctgt aaaccgctta tgccttgttt aagaaaacgg tttcttaaac
     9061 gactacggtg gtcaaggtaa tgaggtttat ctatatggcc ttttactttc atatgttact
     9121 cttcacgatg caaaatgatt tttataactg cattttcttt gcagaaaagc aaaattgcac
     9181 tccttgacaa aactttggtc tctatgttgg cttgtggggc tttggcaatg tgagtcagca
     9241 aaatttaaca tttttgtgcg gtggatagaa attaaaacac attcggatcg gtaatgaatg
     9301 ttgagttgtt tgttagtttc atttttattg tcaatctgct ttgtcgttgt cattttctcc
     9361 aatcgtgcat aacttatcta tatgtgtaac caagttatgc atatttttct taaccaggtc
     9421 cttttttcat tacactatcc cacatgattt ttaatatata agtattcact agtgtcccgt
     9481 taaaagttat tgtcactgaa caggttagga gtatcatcaa t
//
LOCUS       DAONQZ010000079         9328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8048_length_9328_cov_15.656098, whole genome shotgun sequence.
ACCESSION   DAONQZ010000079 DAONQZ010000000
VERSION     DAONQZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_9328_cov_15.656098"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2134
                     /locus_tag="PLR13_10055"
     CDS             68..2134
                     /locus_tag="PLR13_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQC19724.1"
                     /translation="MKTNRLINEKSPYLLQHAKNPVDWYPWGKEAFEKARREDKPVFL
                     SIGYSTCHWCHVMERESFEDDDVAGLLNETFVNIKVDREERPDIDAVYMTVCQVMTQS
                     GGWPLTIMMTPDQEPFFAATYIPRTTRYGRAGMLQIVPRIKEVWKTKRADVLSAASEI
                     TGILNQSATDKGNRLSEETLRQANRSLSMRFDKTFGGFGGAPKFPSPHHFFFLLRHWK
                     RTGDADALHMAEHTLRNMRSGGIYDHVGFGFHRYSTDERWVVPHFEKMLYDQALMAMA
                     FIELFQATGKTEYEATVREIFTYVLRDLTDEQGGFYCAEDADSEGVEGKFYLWTGKEI
                     RGILNEKEADLFLSLYRHPEDGDLPGMHEIPAGCFIPHLTPSAGDKKDLSGLLFSMEY
                     IREKLFSAREKRVHPHKDDKILADWNGLMIAALARGAQVLDEPVYAKAACRAMDFILE
                     TMQTGDGKLLHRYRSGEAAIDANVDDYAFVIRALLELYEATFQTQYLKKALEYNDDLF
                     SNFLDQKNGGLFFTSLKAERLLTRPKELYDGAVPSGNSVAYGNVLKMSRMTGNADMEA
                     KADAICKAFEAPAGAMPEAFTQFLCGLEFAAGPSGQVIITGDLGKPDTRAMLRTLRKQ
                     FAPNNVVIFRPEKPVQPDIETIAPFVKAHLPLNGKATAFVCTDFACHLPTNDPQKMLE
                     LLDTRK"
     gene            2250..2639
                     /locus_tag="PLR13_10060"
     CDS             2250..2639
                     /locus_tag="PLR13_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19725.1"
                     /translation="MKKWLISIMVIAFTLSLAGCNFPNFIKKQSPLQEQCNKQCEAWS
                     KSYMQKYPSDKLTYESHYNKRLNKCFMLVTYSKSQLKSLKEISENKMYGSLLVKQNSK
                     TLICNVLENKCKTEKEWDALVKPYMEE"
     gene            2779..3816
                     /locus_tag="PLR13_10065"
     CDS             2779..3816
                     /locus_tag="PLR13_10065"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HQC19726.1"
                     /translation="MNGLDFLGSMVDEMFLAPGRFIAWLQARRKTDWPAFAREIAFYH
                     EQGFVKNPSSFFVLPDQAPEFVVEKQASYRKGFYQVITFPSRYEPRNPFLRKRYLNHP
                     ENCTGYLIRWIHGDKPRKTVLCVHGFMMGNPREAEQMFKVRKLFSMGLDVALFIHPFH
                     WKRIAGPRASRRVYLTLGDAAFTNECIAHSIHDLHNSFLILHQLGVQDIGLVGASLGG
                     YVTGLYVCLSSLPEFAALMVPAVTFLRPISPALFYNQAPYGPQWRKQVYHAADFHSPL
                     HFYPKIPAEKILVVASRGDRICPADPVEQLRIKWGLSRCHYRTGGHWLVFDKIRGRVW
                     YGLLRDMGFIA"
     gene            4007..4267
                     /locus_tag="PLR13_10070"
     CDS             4007..4267
                     /locus_tag="PLR13_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQC19727.1"
                     /translation="MRVFILILCLFAAFLTFANAAELYRCVDQHGNAIVTDRPQAGME
                     NCITNDVDEEDSPADNKTDEENASADRPENNQEASDHQAAEP"
     gene            4356..4547
                     /locus_tag="PLR13_10075"
     CDS             4356..4547
                     /locus_tag="PLR13_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19728.1"
                     /translation="MSETVISEKDRKMAQRCVECPVCKKARKEQRGFAYWFVKNVEDG
                     LCPNCAAYERVYGKKAHEA"
     gene            complement(4553..5695)
                     /locus_tag="PLR13_10080"
     CDS             complement(4553..5695)
                     /locus_tag="PLR13_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC19729.1"
                     /translation="MNFELPEEMKMLKDMAYKFAVSEIKPVSQECDEHEKYLPEIRIK
                     AAENGLVGAWIPEQYGGPGAGILGNTIITEEISKIDMGISLNIVASGFGCESILNYGT
                     EEQKKTYLPPICSGEKVCAGAYTEPNAGTDVAGYKTRAVKDGKDYVINGNKMFITNGT
                     VCDFMVAQCITHPEEKKHNSFSLIIVPADAKGITRNKIHGKMGIRASDTAEIAFEDVR
                     VPQTNLVGKEGQGFRQLMHFFDITRVMVSGQALGISEACLETSIQYVRERTAFGAPLG
                     SYQMTQMKLTEMAIRIEALRGLVYKAAWLIDQGKPDYTLAAMAKFYGGQTAVFCANYA
                     VELHGGYGYIEEYPVQKWYRDAKILELYEGTKEAEIMTIGRALQAR"
     assembly_gap    5811..5910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5911..6167)
                     /locus_tag="PLR13_10085"
     CDS             complement(<5911..6167)
                     /locus_tag="PLR13_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HQC19730.1"
                     /translation="MKKFSIFIAVIAVIALMGCATMIPIGGLYTATKLPGMATSNTGA
                     ALKTGEAQCTSILGLVAYGDCSISTAKKNGKIKTVHSVDWDA"
     gene            complement(6437..7069)
                     /locus_tag="PLR13_10090"
     CDS             complement(6437..7069)
                     /locus_tag="PLR13_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQC19731.1"
                     /translation="MCGRFALVTDLKSIQKHFNIQEVFCQYQPSWNVLPGQLIPAVIN
                     DHGKKRLVCFRWGLIPSWSKNPSPADGLINARAETVDRKPSFKDALKKRRCLIVADGF
                     YEWKKEDNKKIPFYYFLKSGRPFGFAGLYDTWLSPDKKEISTCTIITTAANEFIFPVH
                     DRMPVILSPEREKIWLAGETNDVSVLKSVLKPYPSGEMDAKPGQGPGSIK"
     gene            7486..8061
                     /gene="lexA"
                     /locus_tag="PLR13_10095"
     CDS             7486..8061
                     /gene="lexA"
                     /locus_tag="PLR13_10095"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQC19732.1"
                     /translation="MDIHAVEQKFAAFFREQRRMPTYSEMLPLLGVKAKSAAEYWTKK
                     LLAKGALEKDAKGFLKPAHWSLNLPLVGNVAAGFPSPAEEELRDTISFDEYLVNNRAS
                     SFVLSVTGDSMIGEGIKEKDLVIVERGREPKNGDIILAEVDGRWTMKYFHKKGKKVTL
                     EAANPKYPPIIPQEELRIAGVITAVVRKYHK"
     gene            8182..9162
                     /locus_tag="PLR13_10100"
     CDS             8182..9162
                     /locus_tag="PLR13_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HQC19733.1"
                     /translation="MPISTTDKKPKLLTQLSQAIRSRHYSRKTEATYIHWIKRFIFFH
                     HVRHPKDMAEPEINAFLTHLAVKEHVSASTQNQALCAIIFLYKYVLNRKIGDIGEVIR
                     AKQSRYIPVVMTKDEVKAVVGNLTGDKKLMISLMYGTGLRLMECLRLRVQDIDFGRNE
                     ITVRDGKGAKDRITMLPESLKEPLRQHLVKVKSVHESDIKDGWGYVQMPYALDRKYPN
                     APRDWRWQWVFPQEKRWKNTKTGEQGRHHTHETLLQKAVKEAVMKSGIAKRIGCHTFR
                     HSFATHLLENGYDIRTIQELLGHKDISTTMIYTHVLSKGGHGVRSPFDSL"
BASE COUNT         2404 a         2191 c         2298 g         2335 t
ORIGIN      
        1 aaagacgcga ttgaaaaagc attttccgcg gcgctgaaat aaatcgaagc aagtttctgg
       61 cacaaaaatg aaaaccaacc gcctgatcaa cgaaaaaagt ccttatctgc tgcagcacgc
      121 aaaaaatccg gtggactggt acccgtgggg aaaagaagca tttgaaaaag cccggcggga
      181 agacaagccg gtttttcttt ccatcggcta ttccacctgc cactggtgtc atgtcatgga
      241 acgagaatct ttcgaggatg acgacgtcgc cgggctgctg aacgagacgt ttgtgaatat
      301 caaggttgac cgggaagagc gtcccgacat tgacgcggtc tacatgaccg tctgccaggt
      361 catgacgcaa agcggcggct ggcccctgac gatcatgatg acgccggatc aagaaccgtt
      421 ttttgccgca acctacattc ccagaacaac cagatacgga cgcgccggaa tgcttcagat
      481 cgttcccaga ataaaagagg tctggaaaac aaaacgcgcc gatgttcttt ccgccgcgtc
      541 ggaaattacc ggcatcctga atcaatccgc caccgacaag ggcaatcggc tgtcggaaga
      601 aacgctgcgg caggctaacc ggtctttgtc tatgcgtttt gacaaaacat tcggcggctt
      661 cggcggggct ccgaaatttc cctcgccgca tcatttcttt tttctgctgc gccactggaa
      721 gcgcaccggc gacgcagacg cgctgcatat ggccgagcat acgctccgaa acatgaggag
      781 cggcgggatt tacgatcatg tcgggttcgg ttttcatcgc tattcgaccg acgaacgctg
      841 ggtggtgccg cattttgaaa agatgctcta cgatcaggcg ctgatggcca tggcctttat
      901 cgaactgttt caggcgacgg gaaaaacgga atatgaagca acggtccgcg agatttttac
      961 ctacgtctta cgtgatttga cggatgaaca gggcgggttt tactgcgctg aagacgccga
     1021 cagcgaaggc gtggaaggaa aattttatct gtggacggga aaggaaatcc gcggcatcct
     1081 caacgaaaaa gaagccgact tgttcctgtc actttatcgc catcctgaag acggcgattt
     1141 gccgggcatg catgaaattc ccgccggttg ttttatcccc catctgacgc cgtccgccgg
     1201 ggataaaaag gatttatcgg gattgctttt ttcaatggag tacatccgcg aaaaactttt
     1261 ttccgcgcgg gaaaaacgcg ttcatccgca caaggatgac aaaatattag cggactggaa
     1321 cggtctgatg attgcggcgc tggcgcgcgg cgcacaggtc ttggacgaac ccgtttacgc
     1381 taaagccgcc tgccgggcga tggatttcat tctggaaacc atgcagaccg gggacgggaa
     1441 actcctgcac cgttaccgca gcggcgaggc cgcgattgat gccaatgtag acgactacgc
     1501 tttcgtcatc cgggcgttgc tggaacttta tgaggcgacg tttcagacgc aatatctgaa
     1561 aaaagctctt gagtataacg atgatctgtt tagtaatttt ctcgatcaaa agaacggcgg
     1621 attatttttt acttccctga aggcggaaag gcttttgacc cggccgaagg aactttatga
     1681 cggcgccgtc ccgtccggca attccgtggc ctacggcaat gtgctgaaaa tgtcccggat
     1741 gaccggcaat gccgatatgg aggcaaaggc ggatgcaatt tgtaaagctt ttgaagcccc
     1801 ggcgggtgcg atgcctgagg ccttcacaca atttctctgc ggtctggagt ttgcggcggg
     1861 accttccggc caggtcatca ttaccggaga tttgggaaaa ccggataccc gggcaatgct
     1921 gcggactttg agaaaacaat ttgccccgaa caatgtcgtt attttccggc cggaaaaacc
     1981 ggtgcaaccg gatattgaaa ccatcgcgcc gtttgtgaaa gcccatttac cgctcaacgg
     2041 gaaagcaacc gctttcgtct gcaccgactt cgcctgccat ctgccgacga acgatccgca
     2101 aaaaatgctt gaacttttgg atacgagaaa atagtattgt tttgtatttt ccgtttgccg
     2161 gcgcttctca aaaccaagcg ttaaaaaagc gcctcaaagc agagcaatcc gaaattccat
     2221 gccggtcacg gttcttcagg aggcacatca tgaaaaaatg gttgatatct attatggtca
     2281 ttgcgtttac actatcactg gccgggtgca attttcccaa tttcataaaa aaacaatctc
     2341 cgttacagga gcaatgtaac aaacagtgcg aggcgtggtc aaaatcttac atgcagaaat
     2401 acccgagcga caaattaacc tatgaaagcc attacaataa gcgattgaat aaatgtttta
     2461 tgctggtaac ctactcaaaa tctcagttga aaagtttgaa agagatcagt gagaataaaa
     2521 tgtacgggtc cctccttgtc aaacagaaca gtaaaacgct tatttgcaac gttctggaaa
     2581 ataaatgtaa aacggaaaag gaatgggacg cgcttgtaaa accttatatg gaagaatagt
     2641 cgtgcaacaa aaaacggcat gataaaaaag tcgattttaa acaacgacct cttttttcat
     2701 cgaatttccg tcgacacaac atcatgattg atattccatt gacaggggaa attcttgttt
     2761 atatattcgc gtatgcgaat gaacggactg gattttttag gcagtatggt tgacgagatg
     2821 tttctggcgc cgggccggtt tattgcgtgg ctgcaggctc gccggaaaac agactggccg
     2881 gccttcgccc gtgaaattgc cttttatcac gagcagggat tcgtcaaaaa tccttcctct
     2941 ttctttgtgt tgcctgatca ggcgccggag ttcgtcgttg aaaaacaggc gtcttatcga
     3001 aaaggttttt atcaggtcat tacttttccc agccgatatg aaccccggaa tcctttttta
     3061 cgaaaacgct acctcaatca tcctgaaaac tgtaccggct atctgattcg ctggatacac
     3121 ggcgacaaac cccgcaagac ggtattgtgc gtgcacggtt ttatgatggg caatccgcgg
     3181 gaagcggagc agatgttcaa ggtccgcaaa ttattttcta tgggcctcga tgttgcgctc
     3241 tttattcatc cttttcactg gaagcgaatt gcaggccccc gcgcgtcgag acgcgtttat
     3301 ctgacgctgg gcgacgccgc ttttaccaac gaatgtatcg cacacagcat tcatgatctg
     3361 cacaattcct ttctcattct gcatcaactg ggtgttcagg acatcggcct ggtcggcgcc
     3421 agtctgggcg ggtatgtcac gggattgtat gtctgtttga gcagtctgcc tgaatttgca
     3481 gccctgatgg tgccggcggt gacatttctg cgacccattt ctccggccct gttttataat
     3541 caggcgccgt acggtccaca gtggcgcaaa caggtttatc acgccgccga ttttcattcg
     3601 ccgcttcatt tttatccgaa gatcccggct gaaaaaatac tggtagtggc atcgcgcggc
     3661 gaccggattt gtcccgccga tccggtggaa caactgagaa ttaaatgggg cttgtcgcgc
     3721 tgtcattacc ggaccggcgg gcactggctg gtcttcgaca aaatccgcgg ccgggtctgg
     3781 tacggtctgc tccgggacat gggatttatt gcctgaacaa cgagccggtt ttgccggcta
     3841 tgaatcaggc caataaaggg gcttgttttt cttccggata agaattaaaa acaaagcatc
     3901 accagagaag acgaagggga agatacttcc cgaaaaagga tcttacggag aaaatccggc
     3961 tgtgaaatgg tacgttaccg ttgcccggat ccggaaggag tgtggcatgc gagtattcat
     4021 tttaatcctg tgtttgttcg cggctttcct gaccttcgcc aacgccgcag agctgtacag
     4081 atgcgttgac caacacggca acgccatcgt cacagaccgc ccgcaggctg gaatggaaaa
     4141 ctgcattacg aatgatgttg atgaagaaga ttcgcctgcg gacaataaaa cagacgagga
     4201 gaacgcctcg gcggaccggc cggaaaataa tcaggaagcg tcggatcatc aggcagcaga
     4261 accttgaggc cggtaaaata cgcagttgct cagacagtgg ataaaatcat gacgttatca
     4321 tcaaatgaag taaatctttt tttccggagg ccgatatgag tgaaaccgtg attagcgaga
     4381 aagaccgaaa gatggcgcaa cgttgtgtcg aatgcccggt gtgcaaaaag gccagaaaag
     4441 agcagcgggg attcgcctac tggtttgtta aaaatgtcga agacggcctg tgcccgaact
     4501 gtgcagcgta tgaaagagtt tacggcaaaa aagcccacga agcctgatcc ggttatctgg
     4561 cctgcaaagc ccggccgatg gtcatgatct cagcttcttt cgtgccttcg tagagttcca
     4621 gaatcttcgc gtcgcgatac cacttctgca cgggatattc ctcgatataa ccgtagccgc
     4681 cgtgcagttc cacggcataa ttggcgcaaa ataccgccgt ctgcccgccg tagaatttag
     4741 ccatcgcagc cagcgtataa tcgggcttgc cctggtcaat gagccaggcg gccttgtaaa
     4801 ccagtccgcg cagagcttca atccttattg ccatttccgt aagtttcatc tgcgtcattt
     4861 gatacgaccc cagcggcgcg ccgaaagcgg tcctttctct gacgtattga atgctggttt
     4921 ccagacaggc ctctgaaatg ccaagcgcct ggcccgaaac cataacgcgg gtgatgtcga
     4981 aaaagtgcat gagttgacgg aacccctgtc cttccttacc gaccagattg gtttgcggaa
     5041 cgcggacatc ttcaaacgct atttccgccg tgtcgctggc gcggataccc attttcccgt
     5101 gaattttgtt gcgggtaatc cctttggcat ccgccggaac aatgatcaga ctgaagctgt
     5161 tgtgtttctt ttcttccgga tgggtgatgc actgtgcgac catgaaatcg caaacggtgc
     5221 cgttggtgat gaacattttg ttgccgttaa tcacatagtc ttttccgtct ttgaccgcac
     5281 gggttttgta cccggcgaca tccgttccgg cattgggttc cgtataagcg ccggcgcata
     5341 ctttttcgcc cgagcatatc ggcggtaaat aggttttttt ctgctcttcc gttccgtaat
     5401 tcagaatgga ctcgcagccg aaaccggacg cgacaatatt caggctgatc cccatgtcta
     5461 ttttggatat ttcttcggtg atgatggtgt tgcccaggat tccggcgccg ggtccgccgt
     5521 attgttccgg aatccacgcc ccgaccagac cgttttcagc ggcctttatt ctgatttccg
     5581 gcagatattt ttcatgttcg tcgcattcct gcgaaacagg ttttatttcc gagacggcaa
     5641 atttataagc catgtccttt aacattttca tttcttcagg cagttcgaaa ttcattttgt
     5701 gtctcctttt atcaataatc tgtttgccgt gaaggcttta ggggtacata ttgcagaaat
     5761 gtatagaaga agtaacaaaa tctgtctggc gaagtcaagc gaaaaccggc nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcatcccagt caacgctgtg cacggttttg
     5941 atcttgccgt ttttcttggc ggtactgatg ctgcagtcgc cgtacgcaac cagtcccagg
     6001 atgctggtac actgggcttc gccggtcttg agtgccgcgc cggtattgct ggttgccata
     6061 cccggcagtt ttgtcgccgt gtagagtccg cctatcggta tcatggtggc gcaccccatc
     6121 agcgcgataa cggcaatgac cgcaataaaa attgaaaatt ttttcatggc acctccttga
     6181 gaatttaagg tttaaattga gttaacctct tgtacggata gctgtttgtt aaaccagact
     6241 cggataaatc aatcttccta aaatttacca ccattccttt tgatattcaa gagttttaca
     6301 tggaaatttt acaaggttat aaatttttta tggagaaaaa aatttctttg tgatgaaatg
     6361 ccgtccggct ttccgcaaaa gagccggaag aaaaatcgta gtaagatgca cgtcatcccg
     6421 taaatataga aaaagattat ttgatgcttc ccggaccctg tcccggtttt gcatccattt
     6481 ctccggaggg atagggtttc agaacagatt tgagaacgga aacatcgttt gtctcgccgg
     6541 ccaaccagat tttttcccgt tccggagaca agatcaccgg catgcggtca tgcacgggga
     6601 aaatgaactc gttggcggcc gtggtgataa ttgtgcaggt gctgatttct tttttgtccg
     6661 gcgacagcca tgtgtcgtaa agtcccgcaa acccgaaggg ccgtcctgat ttcagaaaat
     6721 aataaaacgg tattttcttg ttgtcttctt tcttccactc gtaaaaaccg tcggcgacaa
     6781 tcaggcagcg ccttttttta agcgcatcct taaaactggg tttccggtcc acggtttcgg
     6841 cgcgggcgtt aatcaagccg tcggcagggg agggattctt agaccatgaa ggaatcaggc
     6901 cccagcggaa gcagaccagc cgtttcttcc cgtgatcgtt gatgacggcg gggatgagct
     6961 ggccgggcag aacgttccaa ctgggctggt attggcagaa gacctcctga atattgaaat
     7021 gcttctggat actcttcaaa tcggtgacca gtgcaaatcg tccgcacata cttttatcct
     7081 taagttgctt gcagtcatac cggcgcaggc cggaatccag tcagttaaaa ttataccaga
     7141 tttcccggct gaccgtcctt ttaaatttga gcgtcattaa aactcgggca tcatccccat
     7201 tcgctccgct tcggggataa tttccaatcc cgatataccg ggattggata attccaccaa
     7261 cgcttcgcgt gtcgtttctc tcgcgctcag cgggtgtcat taccacttac gctttaatga
     7321 gccttgattt tccgactcgc agtgatcgga aaatcttaga gcgaattatc ctgccacctt
     7381 taagtggcag ggacaaactg cactgcgttt gtttttggcg agtctcatta ttttttcttg
     7441 acaataggac gttcgtccta tatgctgttt gccatgaaga aacgaatgga cattcatgcg
     7501 gtggaacaaa aatttgccgc atttttcaga gaacagagaa gaatgcccac ctattcggaa
     7561 atgcttccgc ttctgggcgt caaggccaaa agcgccgccg aatactggac caagaaactg
     7621 ctggccaaag gcgctctgga aaaagatgcc aaaggatttc tgaagccggc gcattggtct
     7681 ttgaatcttc ccctggtggg caatgtggcg gccggctttc cctctcctgc ggaagaagaa
     7741 ctgcgcgata cgatttcttt cgatgaatat ctggtcaaca accgggcgtc gtcttttgtt
     7801 ctctctgtga ccggcgattc catgatcggc gaaggcatca aagagaaaga cctggtcatt
     7861 gtggagcgcg gccgggaacc gaaaaacggg gatatcattc tggcggaagt ggacggtcgc
     7921 tggaccatga agtattttca caaaaaaggg aaaaaagtga cactggaagc ggccaacccg
     7981 aagtatccgc cgattattcc tcaggaagaa ttacgtatcg ccggcgtgat tacggcagtg
     8041 gtgaggaagt accacaaatg aggttttgtc ataatcaaga ttgatagagg gagattaaaa
     8101 atgattcaac ttggaattaa tcaatcaaca tctttcaaag gaaagcctgc ggcggcaacg
     8161 tctccggcaa taagagtcgt tatgccaatc tcaacaacgg ataaaaagcc gaaactcctt
     8221 actcaactta gtcaggcaat acgctccagg cactatagcc gtaaaaccga agcgacctat
     8281 attcactgga tcaagcggtt catctttttc catcatgtcc ggcatcccaa agacatggct
     8341 gagccggaaa tcaatgcttt tttaacccat ttggctgtca aggaacatgt cagcgcgtca
     8401 acgcaaaatc aggcattatg cgcaataatt tttctttaca agtatgtttt aaaccgcaaa
     8461 attggtgata taggagaagt tatccgcgcg aaacaatcaa ggtatatccc tgtggtcatg
     8521 acgaaagatg aagtcaaagc tgttgtcggc aatctcacgg gcgataaaaa actgatgatt
     8581 tctctcatgt atggaaccgg gttgaggtta atggaatgtt tgcggctacg tgtccaggac
     8641 attgattttg gtcgaaatga gattacggta cgtgacggta aaggagcaaa agacaggatc
     8701 accatgcttc cggaatcatt gaaagaacct ttgcgtcaac atcttgtaaa ggttaaatcc
     8761 gttcacgaaa gtgacataaa agatggttgg ggctacgtac aaatgcctta tgcactggac
     8821 agaaaatatc ctaatgcgcc tcgtgactgg cgttggcagt gggtgtttcc gcaggaaaaa
     8881 agatggaaaa atacaaaaac cggtgaacaa gggcgccatc acacgcatga aactcttctg
     8941 caaaaggcag taaaagaagc ggttatgaaa tcgggcattg ccaaacgtat cggttgccat
     9001 acttttagac attcttttgc cacgcatctc ttggaaaacg gttacgacat acgaacaata
     9061 caggaacttc taggccataa ggatatcagt acgaccatga tttataccca tgttttaagc
     9121 aaaggaggcc atggggtaag aagtcctttt gacagtcttt agccggttta tacagtctgt
     9181 ataaaccgga ataaaaaaca tatcaatcat gatttaaaca gttgaattta tttaatgata
     9241 ctgataatgt ccgaaatata gaaaaacgat ttatacagaa gggaactgtc tgtgtttaca
     9301 gaattataca gactgtataa acattgtt
//
LOCUS       DAONQZ010000080         9221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8169_length_9221_cov_15.758237, whole genome shotgun sequence.
ACCESSION   DAONQZ010000080 DAONQZ010000000
VERSION     DAONQZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8169_length_9221_cov_15.758237"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..435)
                     /locus_tag="PLR13_10105"
     CDS             complement(28..435)
                     /locus_tag="PLR13_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQC19734.1"
                     /translation="MKWPKEFFRYAVVGFAANVFGFLLYALLTELGVSPVLTISIFYP
                     VHIGIAFYFNRKWSFNFKGHLSASAVRYLAAYVGCYGLNVAMLKFFNGYLGYSHLIVQ
                     AIAIVVIAALLFLAQKMWVFRAQGGSFPFERSL"
     gene            complement(441..2405)
                     /locus_tag="PLR13_10110"
     CDS             complement(441..2405)
                     /locus_tag="PLR13_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19735.1"
                     /translation="MSVFIIRLLMAPLQYDYVDNPFFILAQLSTMEGRPMSPPTAFVN
                     IVGGLFLKLNPTPYIYFWAMIGGALCNALTAGFLGLLASRIFRGSHYLIIAGVLVSFV
                     GSPFDNMDAIIQYYTLSRLLVIIYLYLFIYLVSRETDMDYPYLFLGMLNAMLIFCLPQ
                     LIFYLFIPLIFFSIESFLFKSQKFRWKEFLFILVGFFITLLLMLGLLEIFGFYAYPEP
                     IFSFHEDAPSLFALMHDNYNYHDLFMNGGLDFVWVILIVLLFVDEHKFKTSKSFLFCL
                     TLCLPICYLILKFVSVYENDIHGIFQMQFALYSYLNRIMLLFPCYLVILFTIYYFYPD
                     WKKIFSDQGFNYHLANEKFSMEFKLKMSHQQNEESQGFLASGVSPRHSLKIFLKKFSK
                     LSVLNFVGISTVCCFVVLTFHVFSSMGLYRAYYMQPIALLFFLIPAIQLSIYRKKRIY
                     IISFCIFMMVASLLIQNTFSYSQPLKTSFTDAFISAPLRGLFYEKKYVLTQNEFIQEA
                     SQYIQPGTKIFMPSQEYGLLLALNARPQYVSYFIPGNHLKKLQNGERPEWILLHPQPW
                     WEEVIRKEFRLRFMPEDNLMFAPFSCLQDIKLHLPKQSTLYGYIDMIYWLRKIILPEE
                     YELVIKQEHYRLYRHKGRTKHSHEYENSSG"
     gene            complement(2561..3256)
                     /locus_tag="PLR13_10115"
     CDS             complement(2561..3256)
                     /locus_tag="PLR13_10115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19736.1"
                     /translation="MSIKQQYASRYKGDNTSAVYPVEFVVRAFLGTYPKLNMPRGEYD
                     GRSVLDLGYGDGRNMPLFNNLHMKIHGVEIAEGINRHVGKRMKQLGINADLKVGSNAH
                     IPYPDQYFQYVVACHSCYYVEPGDRFEDNLTEIARVLESGGWLIASLPMVGSYILKDA
                     KPLPGGHYEITKDPYGLRNGVIFRVFHSRQEIRKVFSPFFDHFNIGYCNDLFWGIHQK
                     VWILVCKRKGHKG"
     gene            complement(3282..4229)
                     /locus_tag="PLR13_10120"
     CDS             complement(3282..4229)
                     /locus_tag="PLR13_10120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19737.1"
                     /translation="MKLSIVTTLYQSSSFIPEFVRRVSAEAKKITGDYEIVMVDDGSP
                     DNSLAQALALQSADPHLRIIELSRNFGHHKAMMAGLECASGDFVFLIDVDLEEPPELL
                     TRFYDELKAGNWDVVYGLQKERKGGLIKKFGGRFGWWLIRLLVSVDMPFNLCTVRLMR
                     SSYVRQLIRHKEHMTAIGGLWALTGFRQHGLVFDKLSRGESAYSFVKRLKALLDSVTS
                     FSHVPLYGVFFLGLAIFFVSFLVSVALIIRRLTGYVLDGWISVMISVWSLGGLILLCI
                     GLVGLYISRIFIETKNRPYVIIRNVFDADVPDRKPEEKN"
     gene            complement(4226..5143)
                     /locus_tag="PLR13_10125"
     CDS             complement(4226..5143)
                     /locus_tag="PLR13_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19738.1"
                     /translation="MKWKKLGQVYCPSETDKYTHAMFPVVEVTDDSRGMLRIYYTHRD
                     KTNYGFPTYLDASIVDDRFSILYNHHEPIIEKAALGNFDDSGVNVTSIVKINGKKRFY
                     YYGWNLGVTVPFRNSIGVAEETEDGGMLKRLYAGPMMDRSKEFPNLCATPCVLKDGDV
                     FKMWFASGEPWILIDGKPAVACHVGYAESENGLDWKREKVPAVGHQGADHVVSTPCVL
                     KDGDIYKMWYSYRGLKYRIGYAESADGRTFQRLDEKVGIGVSPGEWDSDMVCYPCVFD
                     LQSKRYMIYCGNEYGKTGFGMAVLVEEST"
     gene            complement(5179..5844)
                     /locus_tag="PLR13_10130"
     CDS             complement(5179..5844)
                     /locus_tag="PLR13_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQC19739.1"
                     /translation="MMAVISDIHGNFPALQAVLREIEKAGCAQIVSLGDVAGYYCQVN
                     ECIELLREKNIPNIMGNHDYYLAYNQSCPRSNAANVLLQYQREHVMPDHLEWLRQSAA
                     KMECYGMSLVHGGWIDPLDEYLTDVSREYFANERAAYFFSGHTHVQSLSLLDGICHCN
                     PGSVGQPRDADARAAFALFDGEKVYLKRVDYDIDLIAAQMKTCGFDDYFYRGLYKGQK
                     IGA"
     gene            complement(5841..6806)
                     /locus_tag="PLR13_10135"
     CDS             complement(5841..6806)
                     /locus_tag="PLR13_10135"
                     /inference="COORDINATES: protein motif:HMM:NF009402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQC19740.1"
                     /translation="MKKYNVLVTGIGAIIGYGIVRSLRQCRYDVNIVGMDVYPDAVGR
                     RWCDAFLQAVWASDPGYCDFVADMIERYRIDLVFPGLEPEIYKLSDGREHFKGDFSKI
                     ILNNKALIDISRDKWRLYEYLRQHSVRSIRTFVTGEYDLIARELNLPFLLKPRRSSAS
                     KGIVTVECREDFDYWKKKLGDNFMVQEIIGDDEHEYTVGVFGLGDGTFSQSIAFSRKL
                     SREGSTVKARTVDIAALDEEVRRLTALFKPVGPTNFQFRRHQEDYLLLEVNPRFSSSL
                     SLRTAFGFNEPEMCIEYFGEKRMPRPASITEGTAVRYIEDVVNRA"
     gene            complement(6793..8568)
                     /locus_tag="PLR13_10140"
     CDS             complement(6793..8568)
                     /locus_tag="PLR13_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQC19741.1"
                     /translation="MKLSDYVMNFLFEKKISHIFGYQGGSVTHLIDSLHKVKGLTYVQ
                     NYHEQASSFCADAYARVSGGVGVAIATSGPGATNLITGIANAFFDSVPCLFITGQVSQ
                     REMKTKKSIRQQGFQETDIVSIVHPITKYAETVMEPEKIRFFLEKALFYAQSGRPGPV
                     LIDLPHNVQASEINVPLLQSFYDSEEYAGETKRLCHPDPQTVTKIVSLLSEAKRPVIL
                     AGGGLASIRGQGIFEEFYNVCDLPVVCSLRGLDAIDHDDEHFCGFIGSYGNRYANFAV
                     ANSDLLLVLGSRLDERQTGPDKTLFARKARIIHVDVDSDELNHNICEAISVCCDVESF
                     LKEALAAVGGRTSDHKKWLAQIHRWKVQYPSCLAHDQACGVDPNEFLHILSRKVSGKA
                     VICADVGQNLMWVAQSFCLSGGKQLLSSSGHGAMGYSLPAGIGAHCASPNRQIICVMG
                     DGGIQMNLQELQTVFRERIPLKIFVMNNQSLGLIRTYQEKYFNDRCYGSVHGYANPDF
                     EKLAYAFDILYTKICTREDFDGLDRGLEIEKPHLFEVVLSPKTQVVPEPAPRGPVEDQ
                     LPLLDRKELSRLFNMDDPVSKDEKI"
     gene            complement(8568..>9221)
                     /locus_tag="PLR13_10145"
     CDS             complement(8568..>9221)
                     /locus_tag="PLR13_10145"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQC19742.1"
                     /translation="WKGYFDMIAAVDEFILYDDMQYTRRDWRNRNQIKTPQGVQWMTV
                     PVKVKGKYHQTIRETEIDGADWAKLHWKSLCQHYRRAKHFDEIAKELEPLYLQNEYSH
                     LSQLNRTLIEWVCMKLGIRTVIRNSWDYQLMDGKTERLVNLCVQAGGNEYISGPAARG
                     YIEEDVFIESGIRLTWFDYDGYPEYPQLWGGFIHGVTILDLLFNCGSEAPRYMRYVR"
BASE COUNT         2630 a         2325 c         1991 g         2275 t
ORIGIN      
        1 aggaccgact gcaatgaaga caccgtttca taaagaccgc tcaaagggaa atgatccgcc
       61 ctgcgctctg aaaacccaca ttttctgcgc caaaaagagc agcgccgcaa tgaccacaat
      121 ggcaatcgcc tggacgatca gatgagaata gcccagatag ccgttaaaga atttcagcat
      181 ggccacgttt aagccatagc atccaacata ggctgccaga tatcggacag ccgacgctga
      241 aaggtgtcct ttgaaattga acgaccattt cctgttaaaa tagaaagcga tcccgatatg
      301 gacgggatag aagatgctga tggtcagcac cggactaacg ccgagttccg taagcagggc
      361 atagagtaaa aatccaaaaa cattcgccgc aaaaccgacg acggcataac gaaaaaactc
      421 tttaggccac ttcaataggc ttaccccgaa ctgttttcat attcatgtga gtgttttgtt
      481 cttcctttat ggcgataaag gcgataatgt tcctgcttta tgaccaattc atattcttca
      541 ggcagtatta tttttcgcaa ccagtaaatc atgtcaatat acccatatag agtcgattgt
      601 tttggcagat gtagtttaat atcctgcagg caagagaaag gagcaaacat caaattgtct
      661 tccggcataa atcgcaggcg aaattccttc cggataactt cttcccacca gggctgggga
      721 tgcaacagaa tccattccgg tcgctctcca ttctgtaatt ttttcaaatg gttgccagga
      781 ataaaataag acacatattg tggacgcgca tttaacgcaa gaagcaaacc atattcctgc
      841 gatggcataa aaatctttgt tccaggttga atatattgag acgcctcctg aataaattca
      901 ttctgtgtaa gcacgtattt cttttcataa aacagtccac gtaatggggc cgagataaaa
      961 gcatctgtaa atgacgtttt tagtggctgt gaatacgaga aagtattctg aatcaaaagg
     1021 cttgccacca tcatgaaaat acaaaaagaa atgatataaa tgcgtttttt acgatagatt
     1081 gataactgaa ttgcaggaat caggaaaaat agcaaggcaa tcggctgcat ataataagct
     1141 ctataaaggc ccattgaaga gaaaacatga aatgtcaaaa ccacaaaaca gcaaactgta
     1201 ctaattccaa caaaattcaa cacggaaagc ttggagaatt tttttaagaa aattttcaaa
     1261 gaatgacgcg gagacacacc tgaagccaaa aatccttgtg attcttcatt ttgttgatgc
     1321 gacatcttta atttaaattc catgctgaat ttttcattgg caagatgata attaaaacct
     1381 tggtcagaaa agattttttt ccagtcggga taaaaataat aaatcgtgaa taaaatgaca
     1441 agataacaag gaaacaacag cataattcga ttcaagtaac tgtataaagc aaactgcatt
     1501 tgaaagatgc cgtgaatgtc attttcatag acggatacaa attttaaaat gagatagcat
     1561 atcggaaggc acagtgtaag acagaacaga aaagatttcg acgttttaaa tttatgctca
     1621 tctacaaata ataaaacaat cagtattacc catacaaaat cgagcccgcc gttcatgaac
     1681 aaatcatgat aattataatt atcgtgcata agagcaaata aagacggcgc atcctcatga
     1741 aatgaaaaaa tcggttctgg atacgcatag aatccaaata tttcaagtaa tccgagcatc
     1801 agcaacaggg tgatgaaaaa gccgactaaa ataaagagga attccttcca acggaatttc
     1861 tgtgacttga acagaaacga ttctattgaa aaaaaaatca gcgggataaa caagtaaaag
     1921 attaattgag gcaaacaaaa tataagcatt gcattcaaca tccccaaaaa cagatacggg
     1981 taatccatgt ctgtttctcg gctaacaaga taaataaaaa gataaaggta aattataaca
     2041 aggagacgag acaatgtata atattgaata attgcgtcca tattgtcgaa tggcgatcct
     2101 acgaacgaaa ccagaactcc tgcaatgatt agatagtgag agccccggaa tatacgtgac
     2161 gcaagcagcc caaggaaccc cgcggttaat gcattgcata aagcgccgcc tatcatcgcc
     2221 cagaagtaaa tgtatggcgt tggattcagt tttaaaaaca gaccgccgac aatattcaca
     2281 aaagccgtcg gcggcgacat tggtcgtcct tccattgtcg atagctgcgc cagaatgaaa
     2341 aacgggttat cgacatagtc atactggagc ggcgccatca ggagccgaat aataaaaaca
     2401 gacacactca gggtcaagaa aatgcatata tttcgattac gataaacact ggtaattatc
     2461 atatgaatca attcttaggc cattttccag ttcggttatt atattctaag gaaccgttag
     2521 gatacgatgc gcgtatctgg atgcccattt taccaaaaca ctacccttta tgtcctttgc
     2581 gtttacaaac taatatccat actttctggt gaattcccca aaaaagatca ttgcagtatc
     2641 caatattgaa atgatcgaaa aacggcgaaa aaacttttcg gatctcctga cgggaatgaa
     2701 aaacgcgaaa gatgacaccg tttctcaacc cataaggatc tttcgtgatt tcatagtggc
     2761 caccgggcaa tggtttggcg tctttgagaa tatacgatcc gaccatcggc aaagacgcaa
     2821 tcagccagcc accggattcg agaactcgcg caatttcggt caggttgtct tcaaaccgat
     2881 cgccgggctc gacgtaatag caggaatgac aagccaccac atattgaaaa tattggtccg
     2941 gataaggaat gtgtgcgttt gagcctactt tcaaatcagc gttgatcccc aattgcttca
     3001 ttcgctttcc aacatgacgg ttaatgcctt ctgcgatttc cacgccgtga atcttcatgt
     3061 gtaaattgtt gaaaagcggc atgttccgac cgtcgccgta acccaagtca aggacgctgc
     3121 gaccgtcgta ttccccccgt ggcatgttta atttgggata cgtacccaaa aacgcccgca
     3181 caacaaactc caccggataa acggcggaag tgttgtcacc cttgtaacgt gatgcatatt
     3241 gctgttttat ggacataaaa tcttcttcct ttccgggtat ttcaattctt ctcctccggc
     3301 ttccgatccg gcacatcagc gtcaaaaaca ttccggataa tgacgtatgg cctgttcttc
     3361 gtctcaataa aaatgcggga aatgtaaagc cctaccaagc caatacacaa caggatcagc
     3421 cctcccaggc tccacaccga aatcatgacg gaaatccaac cgtccagaac gtaaccggtc
     3481 agacggcgga taataagggc tacgctcacc agaaacgata cgaagaaaat cgccagtccc
     3541 agaaaaaaaa caccgtagag cggaacgtgg ctgaacgatg tcaccgaatc caaaagcgcc
     3601 ttaagtcgtt tgacaaaaga ataagcggat tctccgcgtg acagcttgtc aaaaaccagg
     3661 ccatgctgac gaaatcccgt cagcgcccac aatccgccaa tagccgtcat atgctctttg
     3721 tgccggatta gttgacggac gtaggaggag cgcatcaggc gaaccgtgca aagattaaag
     3781 ggcatatcaa cggaaaccaa tagccttatc aaccaccagc cgaatcttcc gccgaatttc
     3841 ttgatcaagc cgccttttcg ctccttttga agcccgtaaa caacatccca attaccggct
     3901 ttcaattcat cgtaaaacct cgtcaggagt tccggaggct cttccaaatc gacatcaatc
     3961 agaaaaacaa aatcgccgct ggcgcattca agaccggcca tcatggcctt gtggtgcccg
     4021 aaattgcgcg acagttcaat gatgcggaga tgcgggtcgg ctgactggag ggcaagcgct
     4081 tgcgccaggg aattatccgg cgatccatca tcgaccatca caatctcata atcacctgtg
     4141 atctttttcg cttccgcgct aacgcgccgg acaaattccg ggataaaaga cgacgactga
     4201 taaagtgtcg taacaatgga aagtttcatg ttgattcctc aaccagaacg gccatgccga
     4261 atcctgtttt gccgtattca tttccgcagt aaatcatgta tctcttactt tgaaggtcaa
     4321 agacgcacgg atagcagacc atgtctgaat cccattctcc cggcgacacg ccaatcccga
     4381 ccttttcatc cagacgctgg aaagttctgc cgtctgcgga ttcggcatat ccgatccggt
     4441 acttcagccc gcgataagaa taccacattt tataaatatc gccgtcttta agcacgcatg
     4501 gcgtggacac tacgtgatcg gcaccttgat gcccgacggc cgggactttt tcccgtttcc
     4561 agtccagccc gttttccgat tcggcgtatc cgacatgaca ggctaccgcc ggtttgccgt
     4621 cgatcaatat ccagggttct ccggatgcga accacatttt gaagacatcg ccgtccttga
     4681 gtacgcacgg cgtggcgcaa agattgggga attctttcga ccgatccatc atgggacccg
     4741 catataagcg tttcagcatg cctccatctt cggtttcctc ggcaacgccg atggagtttc
     4801 tgaacggcac ggttactcca aggttccaac cataatagta gaagcgtttt ttcccgttta
     4861 tttttacgat cgaagtcacg ttcacgccgc tgtcgtcaaa attgcccaac gcagccttct
     4921 caatgatggg ctcgtggtga ttataaagga tcgaaaacct gtcgtccaca atggatgcgt
     4981 ccagataggt tggaaatcca taattggtct tgtccctgtg ggtatagtaa atgcgcagca
     5041 taccccggct gtcatccgtt acctccacga cgggaaacat ggcatgcgta tatttgtccg
     5101 tctctgacgg acagtaaacc tgtccgagtt ttttccattt cataggtatt ttctcctctc
     5161 cgttaccgtt tcaatcagtc aagcgccgat tttttgtcct ttgtacaggc cccggtaaaa
     5221 atagtcatca aaaccgcagg ttttcatctg cgctgcgatc agatcgatgt cataatcgac
     5281 tcttttcaga taaacctttt ccccgtcaaa cagggcaaaa gccgcgcggg catcagcatc
     5341 tctgggctgc cccaccgagc ccgggttgca gtggcaaatc ccgtccagca ggctcagcga
     5401 ctgaacatgc gtatgaccgg aaaaaaaata cgcggcccgc tcatttgcga aatactcccg
     5461 gctgacatcg gttaaatact cgtccagcgg gtcgatccag cctccatgaa caagcgacat
     5521 cccgtagcat tccatcttcg ccgccgactg cctgagccat tccagatgat ccggcataac
     5581 atgctcccgt tggtattgca gcaacacatt cgcggcgttt gaacgcggac aggactggtt
     5641 atacgccagg tagtaatcat ggtttcccat gatattcgga atattttttt cccggagcag
     5701 ttcgatgcac tcattgacct ggcagtaata tccggcaaca tcgcccagcg atacgatttg
     5761 tgcgcagcca gctttttcaa tctcccgcag caccgcctgg agcgcgggaa aattcccgtg
     5821 gatatcggaa ataacagcca tcatgcgcgg ttcacaacgt cttcgatata tcgaacggcc
     5881 gttccttccg tgatgcttgc cgggcgcggc attctctttt cgccaaaata ctcaatgcac
     5941 atttccggtt cattaaagcc gaacgccgtc cgcagggaaa gcgaagatga aaatcgcgga
     6001 ttgacttcaa gcagaaggta atcctcctga tgacgtctga actgaaaatt cgttggtccg
     6061 accggtttga ataacgccgt cagtcgccgg acttcttcgt ccagtgccgc gatatccacc
     6121 gtgcgtgcct tgacggtgga cccttcccgg ctcagttttc tcgaaaaagc gatggactgt
     6181 gaaaatgttc cgtcacccag tccaaaaacg ccgactgtgt attcatgctc atcatcgccg
     6241 ataatttcct gcaccatgaa gttgtcgccc agcttctttt tccaatagtc aaaatcctcc
     6301 cggcattcga cggtcacgat gcctttagag gcggatgacc gacgcggttt cagcaggaag
     6361 ggaagattga gttcacgggc aatcaaatca tattcacctg tcacaaaagt ccggatagac
     6421 ctgaccgaat gctgccgtaa atactcatac aaacgccatt tatctctgga tatgtcaatc
     6481 agcgccttgt tgtttaaaat aatcttgctg aagtcaccct taaaatgttc ccgcccgtcg
     6541 ctaagcttgt aaatctccgg ctccagaccc gggaaaacca ggtcgatcct gtatcgctca
     6601 atcatgtcag ccacaaagtc gcaatagccg ggatcggaag cccaaaccgc ctgaagaaaa
     6661 gcgtcacacc atcgccggcc cacggcgtcc ggatagacgt ccatcccgac aatgttgacg
     6721 tcataccggc actgtctcaa agaacggaca atcccgtagc cgatgatcgc accgataccg
     6781 gtgaccaata cattatattt tttcatcctt ggaaacaggg tcgtccatat taaaaagacg
     6841 actcaattcc tttctgtcca gcagaggaag ttggtcttcc accgggcccc ttggagcagg
     6901 ctcgggaaca acctgagttt tgggagaaag cactacctca aaaagatgag gcttttcaat
     6961 ttcgagccct ctatccagcc catcaaaatc ttcacgcgta caaatttttg tatataaaat
     7021 atcaaatgca tatgcaagtt tttcaaagtc cggattggca tatccatgaa ccgaaccgta
     7081 gcatcggtca ttgaaatatt tttcctgata agtccgaatc agccccagag attgattatt
     7141 catgacaaat attttcaatg gaattctttc ccgaaaaaca gtttgcaatt cctgcagatt
     7201 catctgaatg ccgccgtcgc ccattacaca gataatctgc cgattgggtg acgcacaatg
     7261 agcgccaatc cccgccggca gggagtatcc cattgcgcca tgcccggagg aactcagcag
     7321 ctgtttcccc ccggagagac aaaaggattg ggcaacccac atcaagtttt gtcccacatc
     7381 cgcgcatatt accgcctttc cggagacttt cctgctcaga atgtgcaaaa attcgttggg
     7441 atcgacgccg caagcttgat cgtgcgccag gcatgaagga tattgaactt tccaccgatg
     7501 aatctgggcc aaccattttt tatggtctga agttctgccg cctactgccg cgagcgcctc
     7561 tttcagaaac gattccacat cgcaacagac gctgattgct tcgcaaatgt tatggttcag
     7621 ttcatctgag tcgacatcga catggatgat cctcgccttt ctcgcaaaaa gagttttgtc
     7681 cggccccgtc tgccgttcgt ccagccttga accgagaacc agcaacagat ctgaatttgc
     7741 cacggcaaaa tttgcatagc gattgccgta ggatccaata aatccgcaga aatgttcgtc
     7801 atcatggtca atcgcatcca gacctctcag ggaacagacg actggaagat cgcagacatt
     7861 ataaaattct tcgaatattc cctggcctct gatcgacgcc agtccgccac cggccaaaat
     7921 aaccggtctt ttggcttccg atagcaggga tacaattttt gttaccgtct gtggatcggg
     7981 atggcacagt cttttcgttt ccccggcgta ttcttcagaa tcatagaagc tttgcagcaa
     8041 cggaacgttg atttcgcttg cctgcacatt gtgcggaaga tcgattaaaa caggtccggg
     8101 acgccccgac tgagcataaa aaagagcttt ttcaagaaaa aaacggattt tttccggctc
     8161 cataaccgtt tcagcatatt tggtgatcgg atgaacaatc gacacgatat ccgtttcctg
     8221 aaaaccctgc tgacgaatgg atttttttgt cttcatctca cgttgactca cctgcccggt
     8281 gatgaaaaga cacggcacgg aatcaaaaaa agcattggca atccccgtga tcagattggt
     8341 cgccccgggg ccgctcgtgg caatggcgac tccgacgccg ccgctcactc ttgcataggc
     8401 gtcggcgcag aaagaggaag cctgttcatg gtaattctgg acataggtca gccctttgac
     8461 cttatgaagc gaatcaatca agtgtgtgac ggaaccgccc tgatatccga aaatatggct
     8521 gatcttcttc tcaaaaagaa agttcatgac gtaatctgat agcttcatca gcgaacatac
     8581 ctcatatacc gcggcgcctc gctgccgcag ttgaacagca ggtccagaat cgtgacgccg
     8641 tgaatgaaac caccccagag ctggggatat tcgggatatc catcataatc gaaccaggtg
     8701 agccggatgc cactttcaat aaaaacgtct tcctcaatat aaccccgtgc ggcaggtccg
     8761 gatatgtatt cgttgccgcc ggcctggacg cacaagttga ccaggcgctc ggttttgccg
     8821 tccatcaact ggtaatccca cgaattacgg atgacggtac gaatacccag cttcatgcac
     8881 acccattcga tcaatgttcg attgagttgt gacagatgcg agtactcgtt ttgaaggtaa
     8941 agcggctcaa gttccttcgc tatttcatcg aaatgttttg cgcggcgata gtgctgacaa
     9001 agagacttcc aatgcaactt tgcccaatcc gcgccgtcga tttccgtttc gcgaatggtt
     9061 tggtgatatt ttcctttcac ctttacgggc acagtcatcc attgcacgcc ctgcggcgtc
     9121 ttgatctggt tgcggttgcg ccagtcgcgc cgcgtatatt gcatatcgtc ataaaggatg
     9181 aattcatcca cggctgcgat catatcgaaa tatcccttcc a
//
LOCUS       DAONQZ010000081         9131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8273_length_9131_cov_13.912847, whole genome shotgun sequence.
ACCESSION   DAONQZ010000081 DAONQZ010000000
VERSION     DAONQZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_9131_cov_13.912847"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PLR13_10150"
     CDS             complement(<1..1765)
                     /locus_tag="PLR13_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQC19743.1"
                     /translation="MKNNSCPRGAATPKHPQTPGKKDLKIIRYDEQLFRTLIEQSLDI
                     ILLINRQGIITYANTRAEKVMDFKKEDIIGNEIYTFIHGDDRAPTARVFNELLNNPDA
                     ELHRTEIRTIAKDKTVRTLEAVGTRVVQDGMQDVVLVNLRDITDRRKAEEALANSEAL
                     YRLLADNITEHIWIMDLNLKFTYISPSVEKIYGYPLEKIKTLKLTNLFTKESYQKLRD
                     TFATELPKAMAASPSPDKPRFVLELQAIHADGHLLWIENHLSFIRDENGKPVSIMGET
                     VDITDRKNVQDKLLEEEQRFRALADQSSDIIIMVNREGIVTYENPSVSILGFSVEERL
                     GNHAFEFLHPDDLETISRAFNLLFSDTNAPVQRSEIRLRHRDGSWRTFEAIGSNLIRN
                     NIIEAAIVNLRDITDRKQSEEALKKSEEKYRLLADHMKDQVWLMDLNMHITYVSPSVE
                     RITGYSADEIINMPWKRILTKESFDRVSDFIAAELPKALKSTSGNRLFKTIELEFILK
                     NGQTVWGECEFSLIRDEQGNPLSVLGESRNITERKLAEDKLQQTLESLKKAVGTTIQV
                     LISALEARDPYTAGHQSRTANL"
     gene            complement(1839..2246)
                     /locus_tag="PLR13_10155"
     CDS             complement(1839..2246)
                     /locus_tag="PLR13_10155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC19744.1"
                     /translation="MKEKRKSKRLHEFNEITVSVIPGDPNLPEKDVVYKYSEDLSATG
                     AKIRGNILLPVDTLLKIDFTLKTLQEKITAQGKVKWIKVVFEDNWYEAGVEFVNTPKE
                     AIKKIEDYISWKQNSSRFMPIWISAKFENAKSK"
     gene            complement(2494..2570)
                     /locus_tag="PLR13_10160"
     tRNA            complement(2494..2570)
                     /locus_tag="PLR13_10160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Met,seq:cat)
     gene            complement(2642..4336)
                     /locus_tag="PLR13_10165"
     CDS             complement(2642..4336)
                     /locus_tag="PLR13_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQC19745.1"
                     /translation="MRKALVYIICLTLISLGTGTLWAKDKYTVAILPFAIHSAENIDY
                     IKQGVEEMLTARISAAEKITVIKKADVRQELQNAQIKDINSQNVLEIGKKFTADYVVW
                     GSITKIGNSISLDGKLTNIEQAKSDLGISSQSSSLDDVIPKINDFSENIISHILGTAP
                     PAQTAVSETSPGPSAASRESQIIAGMRSGGRKAGTLTSVINAEFINAPSPFKRQGFWM
                     SQKIPTELKGMDIGDVNNDGANEIVLIDKNAVYIYQKTQDDLKLLETIKGPSYETYRA
                     VDIADINRNGINEIFVTVTQDTFVKSFVLEFKDGKYEKIASGIRLFLRVIDTPSGMPL
                     LLGQELGFGKPFDTPIYEVIWKDKAYVTDQKMQIPQGLSVYGLNIDNVGTGGGGEKIL
                     ALDDLDYLYILDKTTKSLPSLTSFGFASEKIIWRSEDVYGGSNNFIENLDKNNPDDPR
                     AKSTYINLRILTADTNNDGKKEIIIVKNLSSTARLLKQLKLFTASEIYNLEWDGLGMS
                     ENWRTKKINGYVADYAIKDVDNDGRHEIVLALVMSVGVSLRDRSVLVVYELDAPQQ"
     gene            complement(4423..5085)
                     /locus_tag="PLR13_10170"
     CDS             complement(4423..5085)
                     /locus_tag="PLR13_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQC19746.1"
                     /translation="MTETQTAENYTPLEEIFNSLTHGIGILISLAGMVLMIVFASRYG
                     NVNHIVSCTIFGLTLVMLYTASTLYHSFRKPTLKHVFKICDHSCIYLLIAGTYTPFLM
                     VTLKGMLGWSLFAVVWTLTAAGILFKIFFVYRFNILSTIAYILMGWIIIFAVKPLLHS
                     LPEGGIAFLVAGGLAYTLGTIFYAWKKLPFNHAIWHLFVLGGSICHFLAVILYVIPGP
                     SI"
     gene            5270..6571
                     /gene="purB"
                     /locus_tag="PLR13_10175"
     CDS             5270..6571
                     /gene="purB"
                     /locus_tag="PLR13_10175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQC19747.1"
                     /translation="MIPRYSRSVMSEVWSQENKYRKWLDVEMLACEAMARLGKIPQKS
                     LRNIKNKVAIRTERIDEIEKTTRHDVIAFLTSLTEAVGEDGRFIHMGMTSSDVLDTAF
                     AVQLKEAAELLLEDIDVLLLVLKKRAMEHKNTLMIGRSHGIHAEPITFGLKMALWYQE
                     MQRNRKRLQQAKESVSYGKVSGAVGTFSFIDPSVEDYVCNKLGLKPAPVSSQIVQRDR
                     HAEFFTALAIIASSLDKFAQEIRLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSEN
                     ISGLSRLMRSYAVAALEDVALWHERDISHSSVERVIGPDATIALDFMLSRFTNMMDKL
                     VVYPERMMANLNMTHGIIFSQMVLLALVEKGLTREASYTIVQKHAMRSWTEGLEFRVL
                     LEKDAAVKKYLSKKDMAAIFNLKNFLNHLDFIFERVFKAKF"
     gene            complement(6584..8173)
                     /gene="nadB"
                     /locus_tag="PLR13_10180"
     CDS             complement(6584..8173)
                     /gene="nadB"
                     /locus_tag="PLR13_10180"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQC19748.1"
                     /translation="MEFKTDFLIIGSGIAGLSLAIKAAQLGSVTVVTKKEKFESNTNY
                     AQGGIAAVTDKTDSFAEHIQDTLTCGAGLCIRDVVEFIVREAPPRIRELIDWGVHFTR
                     SATEPALYDLGREGGHNRRRVLHARDFTGQEIERALNEQVSSLPNVLIHENHIGIDLI
                     IGKKEAGQPERCLGAYVLDINQGEVHTYRAKYTILCTGGAGKVYLITTNPDIATGDGL
                     AMAYRAGARIANMEFIQFHPTCLYHPSAKAFLISEAVRGEGGILRLKNGSTFMEKYHP
                     MNSLAPRDIVAKAIDSEIKKSGDEYVLLDVTHLKKDFIVNRFPNIYQRCLEYGIDMAT
                     DPIPIVPAAHYICGGVAVDHYGRTSLPRLFACGEVSCTGLHGANRLASNSLLEAVVYA
                     HRVFTKIAETFKDANHDTSPIALWDSSGTTESDDSVVVTNNWDEIRRCMWNYVGIVRS
                     NKRLERAARRIKLIQNEIHDYYWNFKVTKDLIELRNITTVAQLIVESASSRKESRGLH
                     YNIDYPETRDDFRHDTLLIKS"
     gene            8251..>9131
                     /gene="tsaD"
                     /locus_tag="PLR13_10185"
     CDS             8251..>9131
                     /gene="tsaD"
                     /locus_tag="PLR13_10185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQC19749.1"
                     /translation="MAKRKYKRFYMVVLGIESSCDETAAAVVKNGRLLSNVIASQIDI
                     HSPYGGVVPEIASRKHLEVIHPVIRQALNDAGIALQEIEGIAVTCGPGLIGSLLIGLS
                     TAKALAMALNVPMIGVNHLEGHIAAVFVSGDAPPFPFIALVVSGGHTSVYRVENFRDF
                     RLIGQTRDDAAGEAFDKAAKLLNLGYPGGVVIDRMAREGNPQAVAFPRAMKDSPDFSF
                     SGLKTSLLTMLKKRGRDFDQDELPDIAASYQEAIVDTLVEKTVRAAEENAIDQIVVCG
                     GVAANSRLREKFGQAAA"
BASE COUNT         1999 a         2146 c         2629 g         2357 t
ORIGIN      
        1 ccagatttgc cgtgcgggac tgatggcctg ccgtgtaggg gtcccgcgct tccagagcgg
       61 agatgagcac ctgaatggtg gtgccgacgg cctttttcag actttccagg gtctgttgca
      121 gcttgtcttc ggccagtttg cgctcggtga tgtttctgga ttctcccaga accgacaagg
      181 ggtttccctg ttcgtcccgg attaaactga attcgcattc tccccagacg gtctggccgt
      241 ttttaaggat gaattccagt tcaatggttt tgaaaaggcg attgccggat gtggatttga
      301 gcgccttggg caattccgcg gcaatgaaat ccgaaacccg atcaaacgat tctttcgtca
      361 ggatccgctt ccacggcatg ttgatgattt catcggcgga atatccggta atgcgttcca
      421 cggaagggct gacgtaggtg atgtgcatat tcaaatccat gagccagacc tgatctttca
      481 tgtggtcggc gaggagacgg tacttctctt cgcttttttt caatgcttct tccgattgct
      541 tgcggtcggt gatgtcgcgg aggttgacga tggcggcttc aataatatta ttacgaatca
      601 gattgctgcc aatcgcttca aacgtgcgcc agcttccatc ccggtggcga agccggattt
      661 cacttctttg gacaggcgcg tttgtatcgc taaacagcaa attgaatgcc cggcttatgg
      721 tttcaagatc atccggatgg agaaactcaa aagcatgatt gcccagcctt tcttcaacgc
      781 taaatcccag aatgctgacg gacggatttt catacgttac gattccctcc cggttgacca
      841 tgataatgat gtccgaggac tgatccgcca gggcccggaa ccgctgttct tcttccagca
      901 gtttatcctg tacatttttg cggtctgtaa tgtccacggt ttctcccatg atggatacgg
      961 gtttgccgtt ttcgtcgcgg atgaaactca ggtgattttc aatccacagg aggtggccgt
     1021 ctgcgtggat ggcctgaagt tccagaacaa aacggggttt gtcgggagaa ggggaggcgg
     1081 ccattgcttt gggtaattcc gtggcaaaag tatcgcgcag cttttgataa gactcttttg
     1141 tgaaaagatt cgttaatttg agcgttttga ttttctcgag agggtatccg tatatttttt
     1201 ccaccgaggg gctgatatag gtgaatttta agttaaggtc catgatccag atgtgttcgg
     1261 tgatgttgtc ggccagcaga cggtataacg cctcgctgtt ggccagggct tcttccgctt
     1321 tcctgcggtc ggtaatgtcg cggagattga caagcacgac gtcctgcatg ccgtcctgca
     1381 ccacccgggt gccgacagct tccagtgtcc ggacggtttt atctttggcg atggttcgta
     1441 tttctgtacg atggagttcg gcatcgggat tattcaagag ctcattaaat acccgggccg
     1501 tgggggcccg gtcatcgcca tggatgaatg tgtaaatttc attgccgatg atgtcctctt
     1561 ttttaaaatc catcactttt tccgcccggg tattggcgta ggtgatgatt ccctgacggt
     1621 tgatcagcaa aatgatgtcc agagactgtt caatcaacgt gcggaaaagc tgttcgtcgt
     1681 agcgaatgat tttgaggtct ttcttccccg gggtttgcgg atgtttcggc gtcgcggcgc
     1741 ctcgggggca ggaattgttt ttcatgttca aacctcaacc ggagaagatc gatccgacca
     1801 tcgatcgata ccgtaaaaag cggtcggacg gtatccgctt atttggattt tgcgttttca
     1861 aattttgcag aaatccatat gggcatgaag cgtgaagaat tttgtttcca ggagatatag
     1921 tcctcgattt ttttgatcgc ttccttcggg gtgttgacaa attcgacccc cgcttcatac
     1981 cagttatctt caaaaaccac cttgatccat ttgactttcc cctgggcggt gattttttcc
     2041 tgcaatgtct ttaaggtgaa atcgattttg agaagggtat cgacgggcag gagaatgttg
     2101 cctctgattt ttgcgccggt ggccgatagg tcttcgctgt atttgtagac gacgtctttc
     2161 tccggaagat tagggtcgcc gggaatgacc gatacggtga tttcattgaa ttcatgaagc
     2221 cgttttgatt ttcttttttc tttcatggca atctcattcg taaaaaactc tttgatgaaa
     2281 acaggttacc cctgtgcctt atcgtataaa atccggcata aaaatgtcaa ggtattattg
     2341 ctttttaaaa atcagtgcgc ggtcgtttac ggcagctttg cgggatctca aatgtaagga
     2401 gttgccggag ggttggaaaa ggacgcatca ttcaggacga cgcaatattt cgggaggaca
     2461 aagaagacaa aaaaatgaag aactacttta aaatggtggc ggtgcctgga tttgaaccag
     2521 ggacactacg gatatgagcc gtatgctctg accaactgag ctacaccgcc atgattttca
     2581 aaagagcctg acctcaaatc aggtggcacg atatatcgaa gagcgaatca tttgtcaatc
     2641 attattgttg cggcgcgtcc agctcgtata caaccaaaac gcttctgtcg cgcagcgaaa
     2701 cgccgaccga catgaccagc gccagaacga tctcgtggcg gccgtcgtta tccacgtctt
     2761 tgatcgcgta gtcggctacg tatccgttga tttttttcgt gcgccagttt tccgacatgc
     2821 ccagtccgtc ccattccaga ttgtatattt cgctggcggt gaaaagcttt aattgtttta
     2881 aaagccgcgc ggtcgaggag aggtttttca cgatgatgat ttcttttttt ccgtcattgt
     2941 tggtgtccgc agtgagaatc cgcaggttga tgtaggtgct ttttgcccgg ggatcgtcag
     3001 ggttgttttt gtcgagattt tcaatgaagt tgttgctgcc gccgtaaacg tcttcgctgc
     3061 gccagatgat tttttcgctg gcaaagccga aggaggtcag cgacggcaga gatttggttg
     3121 tcttgtcgag aatgtacaga tagtcgaggt cgtccagcgc caggattttt tcgccgccgc
     3181 cgccggtgcc gacattgtcg atgttcagcc cgtaaaccga taagccctgg ggaatctgca
     3241 ttttttgatc ggtgacatag gctttgtctt tccagattac ttcatagatc ggggtgtcga
     3301 acggcttgcc gaatcccagt tcctgaccga gcaggagggg cattccggaa ggcgtatcga
     3361 tgacccgcaa aaacaggcgg atgccggagg caatcttttc atatttgccg tctttgaatt
     3421 ccaggacaaa ggactttacg aatgtgtcct gtgtgacggt gacgaatatt tcgttgatgc
     3481 cgtttcggtt gatgtcggca atatccacgg cccgataagt ttcataggaa ggtcccttga
     3541 tggtttccag cagtttcaga tcgtcctggg ttttctggta gatgtaaacg gcgtttttat
     3601 cgatgagcac aatttcattg gcgccgtcgt tgttgacgtc tccgatatcc attcccttga
     3661 gttcggtcgg aattttctgg ctcatccaga agccctgacg cttgaacggc gagggtgcat
     3721 tgatgaattc ggcgttgata acggaagtca gggtgccggc ttttcgtccg ccggatcgca
     3781 tgccggcgat aatctgggat tcccgggaag cggccgaggg gccgggcgat gtttcagaga
     3841 cggcggtttg cgccggcggc gccgtgccca gaatgtgact gataatgttt tcggaaaaat
     3901 cgttgatctt cggaatgaca tcatccagcg atgacgactg ggatgaaatg cccaggtcgg
     3961 acttcgcctg ttcgatgttc gtcagtttcc cgtccaggct gatgctgttt ccgattttgg
     4021 tgatgctgcc ccagacgaca tagtcggcgg tgaatttttt gccgatctcc aggacgttct
     4081 ggctgttgat atccttgatt tgggcgtttt gaagttcctg ccggacatcc gcttttttga
     4141 tcacggtgat tttttcggcg gcggagatgc gggcggtcag catttcttcg acgccctgtt
     4201 tgatgtaatc gatgttttcc gcgctgtgaa tggcaaaggg aagaatggcg acggtgtatt
     4261 tatctttggc ccagagcgtg ccggttccga gggaaatcag cgtcaggcaa ataatatata
     4321 ctaaagcttt tcgcatggtt atctcctttg ggatgttatg gaaatttaca gcagggatgt
     4381 tctagcattt cagacgcttt aattcaaggc tgatttaggg gattaaatcg acggcccggg
     4441 aatcacataa agaataacgg ccagaaaatg gcagatgctt ccgcccaaaa cgaacaggtg
     4501 ccagatggcg tggttgaaag ggagtttctt ccaggcgtaa aaaatggtgc ccagcgtata
     4561 ggccagtccg ccggcaacca gaaaagcgat gccgccttcc ggcagggagt gtaatagagg
     4621 tttgacggcg aagatgatga tccagcccat taaaatatag gcgatggtgg aaagaatatt
     4681 gaaacgatag acgaaaaata tcttgaataa gataccggct gcggtcagcg tccagaccac
     4741 cgcaaacagg ctccagccca gcatgccttt gagcgtgacc atcagaaacg gtgtgtaggt
     4801 tccggcaatc agaagataga tgcaggaatg atcgcatatt ttaaagacat gctttaacgt
     4861 cggtttccgg aagctgtgat aaagggtgga ggctgtgtag agcatgacca gcgtcaggcc
     4921 gaaaatggtg caactgacaa tatggttcac gtttccgtaa cggctggcaa aaacgatcat
     4981 caacaccatg ccggccaggc tgatgagaat cccgatgccg tgggtgaggc tgttgaagat
     5041 ttcttccagc ggggtgtaat tttcggctgt ctgtgtttcg gtcataaata ttgattcccg
     5101 tttacgtgga ttgtctcata tgctcatccg gggggggggg ttgtcaaccg gtaatacccg
     5161 atgcgtgcaa gaatagtctt gacggatggg caaaagtcgt aatattactg ccccaaagaa
     5221 atgaaaatgc tgtctttaaa ataaatcaag ccatccaaag gagacggacg tgataccaag
     5281 atattcccga agtgtaatga gcgaggtgtg gagtcaggaa aacaaatacc ggaaatggct
     5341 ggatgtggaa atgctggcct gcgaagctat ggccaggctg ggaaagattc ctcaaaaatc
     5401 actccggaat atcaagaata aggttgccat ccgcacggaa cgtatcgatg aaatcgaaaa
     5461 gaccacacgg cacgatgtga tcgcctttct gacctccctg acggaagccg tcggcgagga
     5521 cggacgtttc attcatatgg gcatgacctc gtcggatgtg ctcgataccg cgtttgccgt
     5581 tcagttgaaa gaggccgcgg agcttctgct ggaagatatc gatgtgctgc tgctggttct
     5641 caagaagcgg gcgatggaac ataaaaacac gctgatgatc ggccggtcgc acgggattca
     5701 cgcggaaccg attacgttcg ggctgaaaat ggcgctgtgg tatcaggaaa tgcagcgcaa
     5761 ccgcaaacgc ctgcagcagg ccaaagaatc ggtcagctac ggcaaggtct cgggcgcggt
     5821 cggcacgttt tcgtttatcg atccttccgt ggaggactac gtctgcaaca agctcggtct
     5881 gaaaccggcg cccgtgtcgt cgcagatcgt gcagcgggac cgtcatgcgg aatttttcac
     5941 ggcgctggcg attatcgcgt cgtccctgga caagttcgcg caggagatcc gtctgctgca
     6001 gcggacggag gtgcgggagg ccgaagagta cttttctccc ggccagaagg gatcttccgc
     6061 catgccgcat aagcgcaatc cggttctttc ggaaaacata tcgggtctgt ccaggctgat
     6121 gaggtcttac gcggtggcgg cgctggagga tgtcgcgctg tggcacgaac gggatatcag
     6181 ccattcgtcc gtggagcggg tcatcggacc ggacgcgacg atcgcgctgg attttatgct
     6241 cagccgcttc accaatatga tggacaaact ggtggtttat cccgaacgga tgatggccaa
     6301 tctgaatatg acgcacggca ttatcttttc gcagatggtg ctgttggcgc tggtggaaaa
     6361 ggggctgacg cgcgaagcct cttatacgat cgtgcagaag cacgccatgc ggtcctggac
     6421 ggaagggctg gaattcagag tgctgctgga aaaagacgcc gcggtaaaaa aatatttgag
     6481 caaaaaagat atggcggcca ttttcaatct gaagaatttt ttaaaccatc tggattttat
     6541 cttcgagagg gtcttcaaag caaaattcta ggctgttgtc ttgtcagctt ttgatcagaa
     6601 gggtgtcgtg ccggaagtcg tcgcgtgttt cgggataatc gatattatag tgcagtccgc
     6661 ggctttcttt tcttgacgat gcgctttcca cgatcagttg cgccacggtg gtgatgttgc
     6721 gcagttcgat caggtctttg gtgactttga aattccagta ataatcgtga atttcattct
     6781 gaatgagttt gattctccgc gccgcccgct ccagccgttt attcgagcgg acaatcccga
     6841 cgtaattcca catgcaccgc ctgatttcat cccagttgtt ggtgacgacg acgctgtcgt
     6901 cgctttcggt ggtgccgctg gaatcccaca acgcgatggg cgaggtgtcg tggttggcgt
     6961 ccttgaaggt ttcggcgatc tttgtgaaga cgcgatgcgc ataaaccacg gcctccagca
     7021 gggaattgct ggccagccgg ttcgcgccgt gcagtccggt gcaggacact tcgccgcagg
     7081 cgaaaagacg ggggagggag gtccggccgt aatgatcgac tgccacgccg ccgcagatgt
     7141 aatgcgccgc cggaacaatg gggatgggat cggtcgccat gtcgatgccg tactccaggc
     7201 agcgctgata aatattgggg aagcggttga cgatgaaatc ttttttcaga tgcgtcacat
     7261 ccagtagcac gtactcgtcg ccggattttt tgatttccga gtcgatggct ttggccacga
     7321 tgtcccgcgg ggccaggctg ttcatgggat gatatttttc catgaacgtc gatccgtttt
     7381 tcaaccgtaa aatgccgcct tcgccgcgga cggcctcgct gatgaggaag gccttggccg
     7441 agggatggta aagacaggtg ggatgaaact ggatgaattc catgttggca attctggcgc
     7501 cggcccggta ggccatggcc aggccgtctc cggtggcgat gtcggggttg gtggtgatga
     7561 ggtacacctt gcccgcgcct cccgtgcaca ggatggtgta tttggcgcga taggtatgca
     7621 cttcgccctg gttgatatcc agaacgtatg cgccgaggca gcgttcgggc tggccggctt
     7681 cttttttgcc gatgatcaga tcgatgccga tgtggttttc atgaatcagg acgttgggca
     7741 gactggagac ctgttcgttg agcgcgcgtt cgatttcctg accggtgaaa tccctggcat
     7801 gcagcacacg ccggcgatta tggccgcctt cccgtccgag atcgtacagg gcgggctcgg
     7861 ttgccgagcg ggtgaagtgc acgccccagt cgatgagttc ccggattcgc ggcggcgctt
     7921 cccggacgat gaactccacg acgtcccgga tgcaaagacc cgcgccgcag gtcagcgtgt
     7981 cctgaatgtg ttcggcaaag ctgtcggttt tatcggtcac ggcggcaatg ccgccctggg
     8041 cataattggt gttggattcg aacttttctt ttttggtgac gacggtgacg gaacccagct
     8101 gcgccgcctt gatggcgagg ctcaggccgg caataccgct gccgatgatt aaaaagtccg
     8161 ttttgaattc cattgctgtt ttccgctaca aaaccatcgc caaattttgt ttacaaaagc
     8221 tgtttgcttc tatataggta attgcggatt ttggcaaaga gaaaatacaa gaggttttac
     8281 atggtcgtgc tgggaatcga atcctcctgc gatgaaaccg cggcggcggt tgtgaaaaac
     8341 ggcaggcttc tgtctaatgt gattgcatcg cagatcgaca ttcattcgcc gtacggcggc
     8401 gttgtgccgg agattgcctc gcgcaagcat ctggaggtta tccatccggt catccgccag
     8461 gccttaaacg acgccggaat cgcgttgcag gagatcgagg ggattgcggt cacctgcggg
     8521 ccggggttga tcggatcgct gctgatcggc ctgtccacgg ctaaagcgct ggcgatggcg
     8581 ctgaatgttc ccatgatcgg cgtcaatcat ctggagggac atattgccgc ggtttttgtt
     8641 tccggagacg ctccgccgtt tccgtttatt gccctggtgg tttcgggagg gcacaccagc
     8701 gtttaccggg tcgaaaattt ccgcgatttc cggctgatcg gacagacgcg cgacgacgcc
     8761 gcaggcgaag ccttcgacaa agcggccaag ctgctcaacc tgggatatcc gggcggcgtt
     8821 gtgatcgacc ggatggccag ggagggaaat ccgcaggcgg tggctttccc gcgggcgatg
     8881 aaggattccc cggatttcag tttcagcggt ttgaaaacgt cgcttctgac catgctgaaa
     8941 aagcgcggac gcgatttcga tcaagacgaa ctgccggata ttgcggcaag ttatcaggaa
     9001 gcgattgtcg atacgctggt ggagaaaacc gtgagagccg cggaggaaaa cgcaattgac
     9061 caaattgtgg tgtgcggcgg cgtggccgcc aacagccgac tgcgcgaaaa attcggtcag
     9121 gcagcggcgg a
//
LOCUS       DAONQZ010000082         9104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8303_length_9104_cov_11.201569, whole genome shotgun sequence.
ACCESSION   DAONQZ010000082 DAONQZ010000000
VERSION     DAONQZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8303_length_9104_cov_11.201569"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..906
                     /locus_tag="PLR13_10190"
     CDS             133..906
                     /locus_tag="PLR13_10190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19750.1"
                     /translation="MLANSGSGFKASYFAPLAAREASHFWFRARNALIVWALGKYGTN
                     VTSFMEIGCGTGFVLQGIARRFPAFRLVGSEIFAEGLTFAAERIPGGEFLQMDAREIP
                     FLEEFDAIGAFDVIEHIEEDETVLKQIQCALKPGGLLLLTVPQHRWLWSAVDDHAMHV
                     RRYEARELHAKVVRAGFRVVRSTSFIVSLLAPMFAYRLMQRGERNTFNPHEGLKMNPL
                     LNNIFEKCLHLERALIQGGISFPIGGSRLVAAIKKVKTD"
     gene            925..2067
                     /gene="rffA"
                     /locus_tag="PLR13_10195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             925..2067
                     /gene="rffA"
                     /locus_tag="PLR13_10195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR02379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQC19751.1"
                     /translation="MDTIPFNKPFIAGKELFYIAQAVIENRHTAGDGPFTKKCQQWLE
                     QKLGCRKALLTHSCTAALEMAAMLAGIQPGDEVIMPSFTFVSTANAFVLRGGVPVFVD
                     VHPDTLNIDESLIEAAVTAKTKAVIPVHYAGVPCRMDTIMEIAGRRGIWVIEDAAQAL
                     LSQYRDQCVGVHGQMICLSFHETKNIICGEGGALLINDPDVIERAEIIREKGTNRSQY
                     FRGQVDKYTWVDVGSSYLPSDILAAFLYAQMENAEQIIAKRNLIFDQYMEGLRPLADT
                     GRIRLPLVIEDSRCNGHLFYIVTQSLKERTELINFLAQRGIYAVFHYVPLHTSPAGQR
                     FGRVHGSLKVTEAVSDCLLRLPLYYEMTPEDVQRVISAIEDFYRQG"
     gene            2079..3590
                     /locus_tag="PLR13_10200"
     CDS             2079..3590
                     /locus_tag="PLR13_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19752.1"
                     /translation="MKSSSLDVIKKLFLYLLIILSVVALARLFFFEYRLLNYPYQWEL
                     REALQLHHGILLSEGKSIYAVSPDPPVVLEPYGPVNPFLLTVAVKIFGKNLSGPRLFS
                     FLAFLILLILIAAAIYRLTHSWMFVIIAIGINTFILSWVRWLLLCRPDSLGGMLLFAS
                     IFVHWLYPFRKAAVGASLFLIILAFFTKTYFALGLMLIPLNYWFFHRNRRMAVLYLIS
                     GSALLLASIAAAQSLTEGLYYLFTFELMSQWVTYSLVHLLETMRGLFVYFLPLFVLIV
                     YAIVKKEFHYSVLNIFWIYILVGIPIFMFLLLNTGGDNYYWYAVIPALTLAGIDVLHK
                     ECQKEKSWFAVAMLVLLFSLMMNKMIISETIKDGMVFPSENLAAQWQPVVNEFDHNAN
                     RVLNDDATAILAIHAGKEPYRVGLGYLNIKNTMEKRFGYKFRDIDAEIASCSYSLIIN
                     PEDESYVKQYYRLDRVHPVPVQLGKSYVLKMYVPGCCQEKTASFNPAGLKNAK"
     gene            3714..4514
                     /locus_tag="PLR13_10205"
     CDS             3714..4514
                     /locus_tag="PLR13_10205"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQC19753.1"
                     /translation="MSMLKKVFLRISGNRAVQGAIENKVQSLQELLGIGSGDGVLSSG
                     EQAVFDILRQKFNPPYCIFDVGANQGQFLRLLLEHIAVDDFTVHCFEPGRETFKMLVN
                     SSSGDQRIRLNNIGLGKEKGEAMLYFDNEGSGLASLTKRKLEHFGIDFNQSENVKIDT
                     IDHYCSENFIKHIHLLKIDVEGHELDVLAGAGRMFDEKSIDMVTFEFGGCNIDTRTFF
                     RDFWHFFRKINMKIMRITPSGYFYPIESYREIYEQFRTTNFIAVSGNS"
     gene            4528..6075
                     /locus_tag="PLR13_10210"
     CDS             4528..6075
                     /locus_tag="PLR13_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HQC19754.1"
                     /translation="MLFNSYLFILVFLPVTAAVFFFLGRSGRSTWALGWLIGASLLFY
                     GWWNPWFVTVLIVSILFNYFWGIAVARGSKSLLAVGIAANLGLLGYFKYANFFVENLA
                     AATGLSIELSPVVLPLAISFYTFQQISYLVDAYHGKISRHDFLSYCLFVTFFPRLIQG
                     PIVYYNELAPQFAQKEGFRLNPRMVAAGITIFFFGLFKKVIFADGLAAYATPVFEAAR
                     HGYVLSLMEAWEGALAYTLQIYFDFSGYSDMAIGLAFLFGIRIPLNFNSPYQSLNVID
                     FWRRWHMSLSRFLRDYLYIPLGGNRKGEAKRFVNLMITMLLGGLWHGAAWTFVLWGGL
                     HGLYLIVNHGWVKIKKRFGYEGKGGRVGRFFSGALTFLAVTVAWVFFRADSAATAFRI
                     IGSMSGQNGFALPEKYLDKFSILAVPLQSWGVDFHSMHYFKGSNEVVYIGIALLVVWL
                     APNVQQIMDLCMPYLNIMAKGRKLAQPPAWLIWSPSYFWAIVLVLCALASVLSMSRAS
                     EFIYFQF"
     gene            6086..7033
                     /locus_tag="PLR13_10215"
     CDS             6086..7033
                     /locus_tag="PLR13_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19755.1"
                     /translation="MRNISDKKYLIFFTVAAGILFMVAALFNYIVDPYNLMGNNPTGV
                     YFVQERQVKEAVWTYPHEGLLIGASKTGYVNPDDLSCYRFYNASMRGMVPEEMFFYLK
                     KYLRHEKLVLVGFDFFMFNEREFPLIRIADWEDLRYAKTEYLLSAETVKSSWKTLKMR
                     RNHEKDYGMKPNGQFEYPGEAQASASADHGQQYHDIIQGLVKNHYDEFAFSRTRMNYV
                     RKIKTLLNEKGIPFAVFINPLNRDVVAALQGIKAHRQFLSWKQEMKSIFPDIYDYSIS
                     RYSAREGFYAADPFHYTNATGIAFLNEIIRDFCPDRSKP"
     gene            complement(7022..8005)
                     /locus_tag="PLR13_10220"
     CDS             complement(7022..8005)
                     /locus_tag="PLR13_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HQC19756.1"
                     /translation="MITVKLSHPPWPILRQTPGQKGLWRDCRFFCNTHVDRCDYWFVL
                     EGLGTKKEQSVCPKDHTILITCEPPTLKTYKSEYLHQFAAVITCNPNIDHPRPIFQQQ
                     ALPWHVGRRQKDHVNVEFSKDYDELKAMTSIPKTKILSVISSSKIMSEGHRKRLDFAK
                     QLKRHFGDKVDLFGRDLNEIEDKWDALADYQYHVALENSAVNDYWTEKLSDAFLAACH
                     PIYYGCPNINRYFDPASLTRIDLDHPEKAIAVIESCIEQKKYESSEQKIWDSREKVLE
                     QYNLFALIADYIEKDQRRTGDRPCPPVNITIKKEPSDSNLMYRFRKIFLRL"
     gene            complement(8002..8601)
                     /locus_tag="PLR13_10225"
     CDS             complement(8002..8601)
                     /locus_tag="PLR13_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19757.1"
                     /translation="MNPHPLKILKAKRLLGKTDRLHLGCGPVRLEGFINIDAVYTPAV
                     DFRCKLSKLYDFFPESSVSEIYICHTMEHLPARFLTLYLKQFHYILKKGGILRISVPD
                     IEKCLIASKKQRWSDAELDLLQGVIGGGQDHKYNYHKLFFWPEYLARKLEAAGFIHVE
                     EYPLRPHFAGKDVMDSSNAAGFEPYGMGLSLNMKGEKPK"
     gene            complement(8725..>9104)
                     /locus_tag="PLR13_10230"
     CDS             complement(8725..>9104)
                     /locus_tag="PLR13_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19758.1"
                     /translation="EGELDWSYPLSVDGHFFARREMTAMALLLSFSAPNSFEDQLQIF
                     KPFFINRRGVGYQKSRIVNIPCNRVQTERKNLSGNLHPDELLEQWQKGYQMDYKKFYG
                     LVNESVHQELALALIPRKTFEAK"
BASE COUNT         2098 a         2132 c         2297 g         2577 t
ORIGIN      
        1 atcatttccc tttgagcggt ctttatgaaa cggtgtcttc attgcagtcg gtcctttgaa
       61 agccatgact ggctttgtcc ctattgttcc tggcagccgg aaaaaagcgg ttccgttccg
      121 atattcgctt cagtgctggc caacagcggc agcggcttca aggcttcata ctttgcgccg
      181 ctggccgccc gggaagcgag ccatttctgg tttagagcac gcaatgcatt gatcgtctgg
      241 gctctcggga aatacggtac aaacgtcaca tcttttatgg aaatcggctg tggcaccgga
      301 tttgtgctgc agggcattgc ccgccgtttt ccggcctttc gtctggtcgg cagtgagatt
      361 tttgcagaag gcctcacctt tgcggcggaa aggattcccg ggggggaatt tctgcagatg
      421 gatgcgcgag aaatcccttt tctggaagaa tttgacgcta tcggcgcttt tgacgttatt
      481 gaacacatcg aagaagacga aaccgtgctg aaacagatcc aatgtgcgct gaagcctggc
      541 ggcctgcttt tgctcaccgt gccgcagcat cgttggcttt ggagtgcggt ggacgatcat
      601 gccatgcatg tccggcgtta cgaggctcgt gaactgcatg ccaaagttgt gcgcgcgggc
      661 tttcgcgttg tgcggagcac ctctttcatt gtaagcttat tagcccccat gttcgcctat
      721 cggctgatgc agcggggaga gagaaacacg ttcaaccctc atgaagggtt gaagatgaat
      781 cccctgctga acaatatctt tgaaaaatgt cttcatttgg agcgggcgtt gattcaaggg
      841 gggatctcat tcccgatcgg cggttcccgg ctggtggcgg caatcaaaaa agtaaagaca
      901 gactgatgat ttgagaggta aaccatggat acgattcctt tcaacaaacc attcattgcg
      961 ggtaaagagc ttttttatat tgcccaggcg gttattgaaa accgccatac agcgggcgac
     1021 ggccctttca caaaaaaatg ccagcaatgg ttggagcaaa aacttggttg cagaaaggcg
     1081 cttctcactc attcctgcac ggccgccctt gaaatggccg caatgctggc aggaatccag
     1141 cccggggacg aagtgatcat gccttccttt acctttgttt ccacggcaaa tgcctttgtg
     1201 ctgcgcggcg gggtgccggt ctttgtcgat gttcatccgg atacgctgaa tatcgacgag
     1261 tctttgattg aggctgccgt aaccgccaaa acaaaagccg tcataccggt gcattacgcc
     1321 ggtgttccgt gccgcatgga tacgatcatg gaaatcgccg gacggagagg aatttgggtc
     1381 atcgaagacg ctgcgcaggc cctcttatcc caatacaggg atcaatgtgt cggtgttcat
     1441 ggacaaatga tctgcctgag ctttcatgag acaaaaaaca tcatctgcgg agaaggcggc
     1501 gccttgctga tcaacgatcc agatgtgatc gagcgtgccg aaatcatcag agaaaaagga
     1561 accaatcgga gccagtattt tcggggccag gtggataagt acacatgggt ggatgtcggt
     1621 tcctcctatt tgcccagtga cattctggcg gctttcctct atgcccaaat ggaaaacgct
     1681 gaacagatta tagcgaaacg aaatcttatc ttcgatcagt atatggaggg tttgcggcca
     1741 ttggccgaca ccggacggat tcgacttcct ctggtgatcg aagacagccg gtgtaacggt
     1801 catctctttt atattgtgac gcaatccttg aaggaacgaa ccgaactgat caattttttg
     1861 gcgcaacgcg gcatttatgc tgtttttcac tatgtgccgc ttcacacctc tcctgccggg
     1921 caacgattcg gacgggtcca tggttccttg aaggttaccg aggccgtcag cgattgcctg
     1981 ctccgcctgc cgctttatta tgaaatgacg ccggaagacg ttcagcgcgt tatttccgcg
     2041 atagaagatt tttatcgtca aggataggaa tccgcgcaat gaaaagcagc tccctggacg
     2101 taatcaaaaa actctttctt tacttgttga tcattctctc tgtcgtcgcc ctggccaggc
     2161 tgtttttctt cgagtatcgg cttttgaatt atccttacca gtgggaactg cgcgaggcgc
     2221 ttcagcttca tcatggcatc ctgctgtcgg aagggaagtc catctacgcc gtcagccctg
     2281 acccccctgt cgtccttgag ccttatggcc ccgtcaatcc gttcctgtta accgttgccg
     2341 taaagatttt cgggaaaaat ctgtcgggac cgcgcctctt ttcttttctt gccttcctca
     2401 tcctgctcat cctgattgcc gctgcgattt atcgtctcac gcacagttgg atgtttgtta
     2461 taatcgccat cgggatcaac acgtttattt tatcctgggt ccggtggctg ctgttgtgcc
     2521 ggccggacag tttggggggt atgctgctct ttgcgtcgat ttttgtccat tggctttatc
     2581 cgttccgaaa agcggccgtt ggcgcaagcc tgtttctgat catccttgct ttttttacaa
     2641 aaacttattt tgccttgggg ctgatgctga ttccgctgaa ctactggttt ttccatcgga
     2701 accggcggat ggctgttttg tatctgattt caggttcggc cttgctcttg gcctccattg
     2761 ccgccgcgca atcgctgacc gaaggactgt attatctctt tacattcgaa ctgatgtctc
     2821 aatgggtgac gtattcgctt gttcaccttc ttgaaacgat gagaggcttg ttcgtctatt
     2881 tcctgcccct cttcgtcctg atcgtctatg caatcgtcaa aaaagaattt cattatagcg
     2941 tcctgaacat tttttggatt tatattcttg tcggtattcc gatcttcatg tttctcctgc
     3001 tgaatacggg aggggacaat tactactggt atgcggttat acccgcgctc accctggcgg
     3061 gcatcgatgt tctgcacaag gaatgccaaa aagaaaaatc atggtttgcc gtggcgatgc
     3121 tcgttcttct gttttctctc atgatgaata aaatgatcat ttcggaaacc atcaaggacg
     3181 gaatggtttt tccgtctgag aatctggccg cgcaatggca gcccgttgtt aatgagtttg
     3241 atcataacgc aaatcgagtc ctcaacgacg atgcgacggc cattttggcg attcatgcag
     3301 gcaaagaacc gtatcgggtc ggccttggct atttgaatat caaaaacact atggagaaaa
     3361 gatttggtta caaattcaga gacattgacg cagagattgc aagttgcagc tattcgttga
     3421 ttatcaaccc ggaagacgaa tcttacgtga aacagtatta tcgtctggac cgagtccacc
     3481 ctgttccggt ccaacttgga aaatcctatg tcttgaaaat gtatgtaccg gggtgttgtc
     3541 aggaaaaaac agcctctttc aatccggccg gcctgaagaa cgcaaaataa tcttgtcttc
     3601 gacaacaaaa ggatatagtc agagggcgaa atttctaatg aatcatttca ctttccggac
     3661 gggaaggcga tggcggatca ggaagtattt catgacggag gagaagaaat caaatgtcga
     3721 tgctgaaaaa agtatttctc cggatctcag ggaaccgcgc cgtccaaggt gcaatcgaga
     3781 acaaggttca gtcgctgcag gaactgttgg ggattggttc cggcgacggc gtgttgtcta
     3841 gcggagagca ggccgttttt gatattctgc gacaaaaatt taatcctccg tactgcattt
     3901 ttgatgtcgg tgccaaccag gggcaattcc tacgcttgtt gcttgagcat attgccgttg
     3961 atgattttac cgttcactgt tttgaacccg gacgggaaac attcaaaatg cttgtgaatt
     4021 cttccagcgg cgatcaacgg atcagactca acaacatcgg gctgggcaag gaaaaaggtg
     4081 aagccatgct ttattttgat aatgagggtt ccggtctggc ttctctgacc aaaagaaaac
     4141 tggagcattt tggtatcgat ttcaatcaat cagagaatgt aaaaatcgac actattgatc
     4201 attactgttc agaaaatttc ataaaacaca tccatttact caagattgat gtagaagggc
     4261 atgaacttga tgtcctcgcc ggggcaggaa gaatgtttga tgaaaagtcc atcgatatgg
     4321 ttacttttga atttggcgga tgcaatatcg acacccgaac atttttccgg gacttttggc
     4381 atttctttcg caagatcaat atgaaaatca tgagaattac gccttcggga tatttttatc
     4441 ccattgaatc ctatagagaa atttatgaac aatttcggac aacaaatttt atagcagtgt
     4501 cggggaattc ataaaggttg ccttcccatg ctctttaatt cctatctttt cattctggtg
     4561 ttcctgccgg tcactgcagc ggtatttttc tttcttggcc gcagtgggcg ctcgacttgg
     4621 gcgttggggt ggttgatcgg cgcttccctt cttttttacg gctggtggaa tccctggttt
     4681 gtgacggtgt tgatcgtctc cattctgttt aactacttct gggggatcgc cgtcgcccgc
     4741 ggttcaaaaa gccttctggc ggttgggatt gcagcaaacc tcgggctgct cggctatttt
     4801 aaatacgcta atttttttgt ggagaacctg gccgcggcca ccggtttatc catcgagctg
     4861 tccccggtcg tgctgccttt ggcgatttcg ttttacacct ttcaacaaat atcctatttg
     4921 gttgacgcct accatggaaa gatcagcagg catgattttt taagctactg tttgtttgta
     4981 acttttttcc cccggctcat ccagggtcct attgtttact ataatgaact tgcgccccag
     5041 ttcgcgcaaa aagaaggatt ccgattgaac ccgcgtatgg tggcggcggg catcaccata
     5101 tttttctttg gcttgtttaa aaaagtgatc tttgccgacg ggttggcggc atacgcgacg
     5161 cccgtatttg aggcggcgcg acatggatat gtgctttcat tgatggaagc atgggaagga
     5221 gctctggcct atacactgca aatctatttt gacttttccg gctactccga tatggccatt
     5281 ggcctggctt ttctgtttgg catccgcatt cccctgaatt ttaattctcc ctatcaatcc
     5341 ttgaacgtca tcgatttctg gcgaagatgg catatgtcgc tttcccgttt tttgcgagac
     5401 tatctttata ttcccttggg cggcaaccgc aagggagaag ccaagcgctt tgtcaatttg
     5461 atgattacca tgctgctggg agggctctgg cacggtgcgg catggacctt tgtactgtgg
     5521 ggcggcttgc acggtttata tctaatcgtc aatcacggat gggtaaaaat caagaagcgg
     5581 tttggttacg aaggaaaagg cggaagggtc ggtcgtttct tctccggagc gcttacgttt
     5641 cttgccgtca cggtggcctg ggtttttttc agggccgaca gtgccgccac agcatttcgt
     5701 attattggtt ccatgtcggg acaaaatggc tttgcccttc ctgaaaaata cctggataag
     5761 ttttccattc tggccgtgcc gcttcagtcc tggggggttg attttcactc catgcattac
     5821 tttaagggca gcaacgaagt cgtttatatc ggcattgctt tgctggtggt ctggctggcg
     5881 cccaatgtcc agcaaatcat ggatctttgc atgccgtatc tgaatattat ggccaaaggc
     5941 agaaaactgg cccagccgcc ggcatggctg atctggagtc cctcgtattt ctgggcgatt
     6001 gtgcttgtcc tgtgcgctct tgcctctgtg ctttcaatga gccgtgccag tgaatttatc
     6061 tattttcagt tttaggtagt ggaccatgag aaatatcagc gataaaaaat atcttatttt
     6121 tttcacggtg gccgccggaa tcttgttcat ggttgcggcc ttgtttaact atatcgttga
     6181 tccgtacaac ctcatgggca acaatccaac cggagtttac ttcgtgcagg agcgtcaggt
     6241 caaagaggcc gtatggacat accctcatga ggggcttctc attggagcga gcaagaccgg
     6301 atacgtcaat cccgacgacc tctcctgcta tcgattttac aatgcctcca tgcgcggcat
     6361 ggttccggaa gaaatgtttt tctatttaaa aaaatacctg cgtcatgaaa aactggtgct
     6421 ggtaggcttt gatttcttca tgttcaatga aagagaattt ccgctgatca gaatagcgga
     6481 ctgggaggac cttcgctatg ccaagacgga atacctgcta agcgcggaaa cggtcaaatc
     6541 gtcatggaaa accttaaaga tgcggcgcaa ccatgaaaag gattacggta tgaagccgaa
     6601 tggacaattt gaatatcccg gggaagcgca ggcgtccgcg tctgccgatc atggtcagca
     6661 gtatcatgac atcattcagg gactggtcaa aaaccattat gatgaatttg ccttttcgcg
     6721 aacacgaatg aactacgtca ggaagatcaa aacccttctg aatgaaaagg gcatcccatt
     6781 cgcggtattt atcaacccct tgaaccgtga tgtcgttgcc gccctgcaag gcatcaaagc
     6841 ccatcgtcag tttttaagct ggaagcagga aatgaagagc attttccccg acatctatga
     6901 ttactccatc agccggtatt cggcgcggga ggggttttat gcggcggatc cttttcatta
     6961 caccaacgcc acgggaatag cgtttttaaa tgagatcatc cgtgatttct gccccgaccg
     7021 gtcaaagccg taaaaatatt ttcctgaacc ggtacatcag attggaatcg gaaggctctt
     7081 tcttaatggt gatgttgacg ggggggcaag gccggtcgcc tgtgcgtcgt tggtcctttt
     7141 caatgtaatc ggcgatcaag gcaaacaaat tatattgttc cagaactttt tccctggagt
     7201 cccagatctt ttgttccgac gattcatatt ttttctgttc gatgcacgat tcaatgacgg
     7261 cgattgcctt ttccggatga tccaggtcaa tccgggtcag agaggcggga tcaaaatagc
     7321 ggttgatgtt ggggcagcca taatagatgg ggtggcaggc cgccagaaaa gcatcggaga
     7381 gtttttccgt ccagtagtcg ttgaccgcgc tgttttcaag cgccacgtgg tattgatagt
     7441 cggccagcgc gtcccatttg tcttcgattt cgttcagatc ccttccaaac agatcgactt
     7501 tgtcgccgaa gtgccttttc agctgctttg cgaaatcaag tctcttccga tgcccctcac
     7561 tcatgatctt ggatgatgaa ataacggaaa ggatttttgt tttgggaatg gaagtcatgg
     7621 ccttgagttc atcataatcc tttgaaaatt cgacattgac atgatctttc tgccttctgc
     7681 cgacatgcca gggcagtgcc tgttgttgaa agatcggccg ggggtgatcg atgtttgggt
     7741 tgcaggtgat aacggcggcg aactgatgaa gatactcgct tttataagtt ttcagcgtgg
     7801 ggggctcaca cgtaatgagg atggtatgat ctttcggaca gacactttgc tcttttttgg
     7861 taccgagtcc ttccaggaca aaccagtagt cgcagcggtc gacatgggtg ttgcagaaaa
     7921 aacggcaatc gcgccaaagt cctttctgtc cgggggtctg acgaaggatc ggccacggcg
     7981 ggtgagagag cttcactgtg atcattttgg cttttcacct ttcatgttca ggctcagacc
     8041 catgccatag ggttcaaaac cggccgcgtt gctggaatcc atgacatctt tgccggcaaa
     8101 gtgaggcctg agagggtatt cctccacatg gataaatcct gcagcttcca gttttcttgc
     8161 aagatattcg ggccagaaaa agagcttatg gtagttgtat ttatggtcct ggccgccgcc
     8221 tatcactccc tgcagcaaat ccagctcggc gtcagaccat cgctgctttt ttgatgcgat
     8281 aaggcatttt tcgatgtccg gcaccgatat gcgcaagatt ccaccctttt tcaggatata
     8341 atgaaactgc tttagataca gcgtaagaaa gcgcgcgggc aggtgctcca tggtatggca
     8401 gatgtatatt tcggagaccg aactttccgg aaaaaaatca taaagtttgc ttaacttgca
     8461 tcggaaatcg accgccggag tatagacggc atcgatgttg ataaatcctt caagtcgaac
     8521 cgggccgcat cccaggtgaa gcctgtcggt tttgccgagc agtcttttgg cttttaaaat
     8581 ttttagagga tgtggattca tccacgattt ttttggtttc agtctgttgg cggccgcccg
     8641 gtcgaaaaac cggagcagcc gatatccgct tcccaaaaat gtccaaataa attcaggtaa
     8701 tttcaagtgg aacccctcat tgcgttattt tgcttcgaat gtttttctcg ggatcagcgc
     8761 taaagcaagc tcctgatgga cgctttcgtt caccagaccg taaaatttct tataatccat
     8821 ctgataccct ttttgccatt gttccaagag ctcgtccggg tgaagattgc cggaaaggtt
     8881 ttttcgctct gtttgaaccc ggttgcaggg gatgttcaca atcctggatt tttgatatcc
     8941 gaccccgcgt cggttgatga aaaaaggctt aaagatctgc agttggtctt cgaaagaatt
     9001 gggagcgctg aaggatagca gtagggccat tgccgtcatt tcccgccttg caaagaaatg
     9061 gccgtctacc gaaagaggat aactccagtc cagctctcct tctt
//
LOCUS       DAONQZ010000083         9026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8391_length_9026_cov_19.109910, whole genome shotgun sequence.
ACCESSION   DAONQZ010000083 DAONQZ010000000
VERSION     DAONQZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8391_length_9026_cov_19.109910"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLR13_10235"
     CDS             complement(<1..659)
                     /locus_tag="PLR13_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0F1 subunit beta"
                     /protein_id="HQC19759.1"
                     /translation="MESEQINQGKVLSVRSSVVDVYFSKVPPIRNVLKAGEKEDVIIE
                     VFTQLDNNTVRGVALTPTQGLARGSKVIDTGRPLEVPVGKELMGRVFNVFGQTIDKKG
                     NVEDVEIRSIHREPIPLRDQSTVSEIFETGIKAIDVLAPLERGGKAGLFGGAGVGKTV
                     LITEMIHNTVSAHEGMSIFCGIGERCREGEELYREMEESGVLGNTVMVFGQMNEPPGA
                     R"
     gene            complement(690..1526)
                     /locus_tag="PLR13_10240"
     CDS             complement(690..1526)
                     /locus_tag="PLR13_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19760.1"
                     /translation="MEAYAAKLIDLIESKADDIARQWAEDVMKHNRTPSYHNLSKPMV
                     IEQGVDFYKLFRRMSMAEHPYEEAKTFSWKYAQKFYDEKIPLQEATYALILLRRNLWL
                     YAEFQGMFFTALEKQQAVESLNRTILMFDYVAYQVIQKYQERIHQDIDTRLFAVETLM
                     KKSCIGGEKKTYQYALMALFVVIAFVITFYYHSVAQTGVIFTHLFYIPIVLASICWGR
                     KGVWVSVFLAVMLLCSHAVFLEGVSFTEDIIRAVMFVVVGGVVGWLMEDVKKVRELFN
                     KR"
     gene            complement(1594..2064)
                     /locus_tag="PLR13_10245"
     CDS             complement(1594..2064)
                     /locus_tag="PLR13_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19761.1"
                     /translation="MKAFTAKLVDLTQTNADEIARQWARDVKKNAKTPSYHQAPEEKI
                     IRQAKEFYNNFQMMFFNESPYEQAKDFFDKYAEERYKEEIPLHEALYALILMRRHMWL
                     YAEFQSLFNVEVDHRQAVESLSRSMLMFDYIMYVVSMKFSKMMQQEVLSRSVKR"
     gene            complement(2414..3781)
                     /locus_tag="PLR13_10250"
     CDS             complement(2414..3781)
                     /locus_tag="PLR13_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQC19762.1"
                     /translation="MRILIVDDEEVALNSIKRLLKWRGIRNVDTCANGKDAIAKIKET
                     DFDIVLLDLLMPEVDGLQVLEAAKPYRPHTEFIILTAIDDIPTTVKAIRLGAYDYLVK
                     PVDNDLLFLAIRRAYEHRGLLIGLSTTAGYDKSSIPAAFAEIVTQCPRMCSLLSYAQI
                     MAGGGNPIMITGESGTGKELIARGIHRAGPMPGGPFIAVNVSAIPASMFESQFFGHAK
                     GAFTGADTPHRGYFEQADGGTLFLDEIGELPVGLQSKLLRVLEEKSFYPLGASKPVWV
                     DVRVVSASNRDVEKACQDGSFRLDLFYRLKSVHIHLPPLREREGDIPLLALHFLRKSR
                     ENYKKTITGFNPEAMELLTARNYPGNIRELAQVVENAVLLADSDFIQPCHLGMVSTAP
                     LPLSRQLGTLKENEESHLVYVLKNVQGDRGRAAQILGITVRQLQRKIAAMKKNPQWET
                     ILNDI"
     gene            complement(3778..5919)
                     /locus_tag="PLR13_10255"
     CDS             complement(3778..5919)
                     /locus_tag="PLR13_10255"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19763.1"
                     /translation="MKTGSSPSRKNKSPGRHESVWTGEVFSNGPQLLQSIIGSSPIPA
                     FIIGKDHLIIYWNRALEELSGIKSKEVMGTGQQWRAFYKTARPCMADLLVDKYQKKIP
                     QWYSGKFIKSKLIDGAYEATDFFPELGKRGKWLRFTSAVIRNSKGSVIGAIETLEDIT
                     QAKRAEEALLKAHEDLEIKVRERTRKLTKTNKALKEAMDHLSLILEFLPIVSYTCNAD
                     ESMAITFVSNRVKEITGYTPAQFTANHTFWRNHIHPEDQSNVLKKLKPNARKKTYQLE
                     YRFLTADGSYRWISDHRRLIKPGKHSRSYMVGAWQDITEDKRIRHEGEFRLQQMIQSH
                     KLKALGEVVAGVAHEINNPVSFIANNITMLEEMWNAVEPILASDGASHPDWSEKGIGY
                     VEVCTNMKEIIEEFKTASQRIKRVISGLKEFARTDETVEKKPVKIEEVIQGVMIIVGA
                     QVRKTVSRIDLYIDSNLPPVQGHFQKIEQVIANLLINAHQAITPERKGRIVITARSLD
                     RLNAILIEIEDNGIGMKKAVLDHIFQPFFTTRRDMEGTGLGLSISYGLIKEHNGLIGV
                     LSKPGVGSRFSIYLPLDARTPISIRPAVLCCDSDTAYLKEIKMSIVNVIDWQCGDQDN
                     CDDIIAYLEMHPEVDIFISEINLPVMNGWDLSRKIKERFPLLCVILYSSDPQAGKPEG
                     RMSGKPDYVLQKPFSMTQLQTIIRETGRQRL"
     gene            complement(6134..6757)
                     /locus_tag="PLR13_10260"
     CDS             complement(6134..6757)
                     /locus_tag="PLR13_10260"
                     /inference="COORDINATES: protein motif:HMM:NF022168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
                     /protein_id="HQC19764.1"
                     /translation="MLYIDRTTLIGRGRHRECYKHPEDQNLCIKITVKDSPLEIKREK
                     KYYRHLQKHGISWEMISRYHGDVETDLGQGSVFDLIMDHDGTVAKSLEYYLSSDEQTE
                     KYFSSLSDALCRLKKYLLQNRIVTMDIKPYNILCQMDASGIARLVLIDNIYNSEFIPV
                     SNYCRFFAKRKIDRKWQRFEDSLLNAFNGNKALARMLKERSVRRSSR"
     gene            complement(6875..7615)
                     /locus_tag="PLR13_10265"
     CDS             complement(6875..7615)
                     /locus_tag="PLR13_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19765.1"
                     /translation="MKKLFLSILVIILTISFMSWVIYMNQAPAIKQASDSKDIVIEPS
                     PRSDDETAQRALLPDEKSKEISADQGAPATGKSVPAKDRAQVVPSTAAKKPAVSAVDE
                     TKKAPPVSGETLKKPQPAPSEKIQATPASEKSQKEMSRLQDQCEKRSKKIFRKAYGNG
                     VVEKNQNVYLYLYKSHYNKKRDQCFMLVTEDGVLERYKKLLDVDENESLGSVRLNNER
                     KNLGCYLLNSQCDSEEAWDLLAKPYMEE"
     gene            complement(7974..8207)
                     /locus_tag="PLR13_10270"
     CDS             complement(7974..8207)
                     /locus_tag="PLR13_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HQC19766.1"
                     /translation="MKDKKTYAVVLFKAVSYALKAEKILKQEGLPHKLIPVPKQISSD
                     CGVCLRFEQNIQNQIEAALLNKVEIEDIITLDH"
     gene            complement(8204..8815)
                     /gene="yedF"
                     /locus_tag="PLR13_10275"
     CDS             complement(8204..8815)
                     /gene="yedF"
                     /locus_tag="PLR13_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HQC19767.1"
                     /translation="MADIKIIDARGLSCPQPVVLTKKALEDNARVTTIVDNETALENV
                     KRLAGKFGFAVTVESQGKGIYEIHIGRGADFNKDQDFVASCQTGAAPSGPFVIAVTED
                     KMGRGDDELGTVLIKSFVHTVADLGKKPDVMIFYNTGVKLTIEGSDVLEDLKQMNSEG
                     VQILVCGTCLNYFEIKDKLAVGTVSNMYDIVETMSGAGRLLTP"
     gene            complement(8817..>9026)
                     /locus_tag="PLR13_10280"
     CDS             complement(8817..>9026)
                     /locus_tag="PLR13_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQC19768.1"
                     /translation="GMDPAAVALELDDRFNIMARSGIHCAPSAHKTIGTYPQGTVRFS
                     FSYFNTQEQIAKAIDAIRKISRRKK"
BASE COUNT         2196 a         2246 c         2033 g         2551 t
ORIGIN      
        1 aaacgcgcgc ccggcggttc gttcatctga ccgaagacca tcacggtatt gcccagaacg
       61 ccgctttctt ccatttcccg gtagagttct tcgccctcgc ggcagcgttc gccgataccg
      121 cagaagatac tcatgccttc atgcgcgctg acggtattat gaatcatctc cgtaatcaaa
      181 accgtcttac cgacaccggc gccgccgaac agaccggctt taccgcctcg ttccagagga
      241 gccagaacat cgatggcttt aatgcccgtt tcaaaaattt ccgaaacggt ggattgatcg
      301 cgcagaggaa tcggctcacg gtgaatggaa cgtatttcta cgtcttcaac attccctttc
      361 ttatcgattg tctgtccgaa aacgttgaaa acccttccca taagctcttt gcccacgggg
      421 acttcaaggg gcctgcccgt gtcaatgact tttgaacctc tggccagacc ctgcgtggga
      481 gtcagggcga cgccccggac cgtattgttg tcgagctggg taaagacctc gatgatgacg
      541 tcctcttttt cgccggcttt gagaacatta cgaatcgggg gcaccttgga aaaataaaca
      601 tcaacaacac tgctgcggac ggataaaact tttccctgat tgatctgttc gctttccacg
      661 ttcatctcct cttaaataaa attgatgctt taacgtttat tgaaaagttc tcttactttt
      721 ttcacatctt ccatcaacca tcccaccacg ccgccgacca caacaaacat tacggcccga
      781 atgatgtcct ccgtaaacga aacgccttcc agaaaaacag cgtgactgca aagaagcatg
      841 acggcgagaa acacagacac ccagacaccc tttctgcccc agcaaataga cgccaggaca
      901 atgggaatgt agaacagatg ggtaaaaata actccggttt gagccacaga gtggtagtag
      961 aatgtgatga cgaaagcgat gacgacaaaa agggccatca gcgcgtactg ataggttttc
     1021 ttttcgcccc cgatgcagct ctttttcatc agcgtttcca cggcgaaaag tcttgtgtct
     1081 atgtcctgat gaatgcgttc ctgatatttc tgtatgacct gataggccac ataatcaaac
     1141 atcagaatgg ttctattcag gctctcgacg gcctgttgtt tctccagagc agtgaaaaac
     1201 attccctgaa attcagcata cagccaaagg ttcctgcgca agaggatcag ggcgtaagtc
     1261 gcttcctgaa ggggaatctt ttcatcgtaa aatttctggg cgtatttcca cgaaaatgtt
     1321 ttggcttctt cgtaggggtg ttccgccatg gacatgcggc ggaacagttt gtagaaatcg
     1381 actccctgtt caatcaccat cggtttcgac aggttatggt aggatggggt ccggttgtgt
     1441 ttcatcacat cctcggccca ttgccgcgca atatcgtcgg ctttgctttc aatcagatca
     1501 ataagtttgg cggcgtaagc ttccatggct tcatctcttc caatacaaac cggcttgata
     1561 aatcatcgac tgcgtcctgc tgatgtctgc ttttcagcgt ttcactgatc ttgacaaaac
     1621 ctcctgctgc atcatttttg aaaatttcat agaaacaacg tacataatgt aatcgaacat
     1681 cagcatcgat ctgcttaagc tttccacggc ctgccgatga tccacctcca cattgaacag
     1741 ggactgaaat tccgcataga gccacatgtg tcttctcatc agaatcaacg cgtacagggc
     1801 ctcgtgaagc gggatttctt ctttgtacct ttcctccgca tacttatcga aaaaatcttt
     1861 tgcctgttca taaggagact cgttaaaaaa catcatttga aaattattat agaattcctt
     1921 agcctgacga ataatcttct cttccggcgc ctgatggtag ctgggagttt tcgcattctt
     1981 ttttacgtct ctggcccact gcctggctat ttcgtccgcg tttgtttgag ttaaatccac
     2041 aagctttgcg gtaaaagctt tcattggtca tcatcctttt caaaagaata aacagcaacg
     2101 ggcatgccaa aactattttc ccctcttgat ttattcgtga ccggcttgtc tatccggaca
     2161 aaaagcgaga cgaaaatcat cagaaacctg tgctattgaa gccttacgaa aaagcggatg
     2221 gaaaaatctt tccggtaaaa aaatattttt tctttgtcat gtcagcggat caggtttttg
     2281 aacggacgag aaaaaaacga aataaaagac ggcggaaagg atatcgatgg acaggccggg
     2341 gactctttaa aacggcaaga ccttttaaaa acaggaactt attcaaaaag acatttatgt
     2401 cgcccctgac gacttaaatg tcattcagga tggtttccca ttgcggattt ttcttcatcg
     2461 cggcaatttt tctttgcagt tggcggacgg ttatgcccag gatttgcgcc gccctaccgc
     2521 ggtcgccctg aacatttttc aaaacgtaca ccagatggga ttcttcgttc tccttgagcg
     2581 tacccagttg ccgggataaa gggagcggtg cggtggaaac cattcccaga tgacacggct
     2641 ggatgaaatc ggaatccgcc agcaaaacgg cgttttcaac aacctgcgcc agttccctga
     2701 tattgcccgg ataatttctg gcggttaaca gttccatggc ttccggatta aagcctgtaa
     2761 tggttttttt ataattctca cgcgatttgc gcagaaaatg gagcgcgagc agagggatgt
     2821 ctccctctct ttctctcaac ggcggcaaat ggatgtgcac ggatttcaaa cggtaaaaaa
     2881 gatccagccg gaaacttccg tcctggcacg ccttctcgac atctcggttg gaagcggaga
     2941 caacccggac atccacccag accggcttgg aagcgcccaa gggatagaaa gacttttctt
     3001 ccagtacccg cagcaatttg gactgcaggc caacgggcag ttccccgatt tcatccagaa
     3061 aaagggtccc tccgtccgcc tgttcgaaat agccgcgatg gggagtgtcc gcgccggtga
     3121 atgcgccttt ggcatggccg aagaactggc tttcgaacat gctggcggga atcgcgctga
     3181 cgttcacggc aataaaagga ccgccgggca tcggccccgc ccgatgaatc cctctggcga
     3241 tcagctcctt tcctgtcccg gactcgccgg taatcatgat cgggttgccg cctccggcca
     3301 tgatctgtgc ataggaaaga agcgaacaca tcctcggaca ctgggtcaca atctccgcaa
     3361 atgcggcggg aatgctgctt ttatcgtaac ctgccgtggt ggataagccg atgagcagcc
     3421 cccggtgttc gtaggcccgt cggatggcca gaaaaagcaa gtcattatcc accggtttga
     3481 ccagataatc atacgcaccg agacgaattg ctttcaccgt ggtgggaata tcatcgatag
     3541 cggtaagaat aatgaattcc gtatgcggcc gatagggttt tgccgcctcc aggacctgca
     3601 aaccgtcaac ttccggcatc agcagatcca gaagaacaat atcgaaatcg gtttccttga
     3661 ttttcgcaat cgcgtctttg ccgtttgcac aggtgtccac atttctgatg ccccgccact
     3721 tcaaaagacg tttgattgaa ttcagagcaa cctcttcatc atctacaatc agtattctca
     3781 taatcgttgc ctgcctgttt cacgtatgat cgtttgcaat tgcgtcatgc tgaagggttt
     3841 ttggagcaca taatccggtt ttcccgacat gcgtccctcg ggtttgcctg cctgcggatc
     3901 ggaggaataa agaatgacac agagcagggg gaaacgctct ttgatttttc gggacaaatc
     3961 ccatccattc atgaccggca gattaatttc cgaaataaaa atgtcaactt cgggatgcat
     4021 ttccagataa gcgatgatat catcgcaatt atcctgatcg ccgcactgcc agtcaatgac
     4081 attcacaatg ctcattttta tttctttcaa ataggcggta tcgctgtcgc agcagagaac
     4141 ggccgggcga atgctgatcg gtgtgcgggc atccagcgga aggtagattg aaaaacggct
     4201 tcccactccc ggcttggaca gcactccgat caatccgtta tgctccttga tcagaccgta
     4261 agagatggaa agtcccagtc cggtcccctc catatcccgg cgcgttgtaa aaaagggctg
     4321 gaaaatatga tccagcaccg cctttttcat gccgatgccg ttatcttcaa tttcaatcag
     4381 aatcgcgttg agccgatcaa gacttctggc ggtaataaca attcttcctt ttcgttccgg
     4441 tgtaatggcc tgatgcgcat tgatcagcag gttggcgatc acctgttcga ttttctggaa
     4501 atggccctgg acaggcggca aattgctgtc gatgtaaaga tcgatgcggg aaacggtttt
     4561 gcgaacctgc gcaccgacaa taatcatcac cccctgaatg acttcttcga ttttcaccgg
     4621 ttttttctcc acggtttcgt cggtgcgggc aaattccttc agaccggaaa taacgcgctt
     4681 gattctttgg gacgccgttt tgaattcttc gatgatttcc ttcatgttcg tgcagacttc
     4741 cacgtagcca atgccctttt cactccagtc gggatgagac gcaccgtcac tggccagaat
     4801 cggttccacc gcgttccaca tttcctccag catggtgatg ttgttcgcga tgaaactgac
     4861 cgggttgttt atttcatgag caacgcccgc gaccacttcg ccgagcgctt tcaatttatg
     4921 ggattgaatc atctgctgta agcgaaattc tccttcatgg cggattcttt tatcttctgt
     4981 tatatcctgc catgctccca ccatgtaact gcgcgagtgt ttgcccggtt tgatcaaacg
     5041 gcgatgatcc gatatccagc ggtaagaacc gtcagcggtc aggaagcggt actccaactg
     5101 gtaagttttt ttcctggcat tcggtttcag ttttttcaga acattgctct ggtcttcggg
     5161 atgaatgtga ttccgccaga aagtatgatt ggccgtaaat tgagcgggcg tgtatccggt
     5221 gatttccttg acgcgattac tgacaaacgt gatggccatg ctttcgtcgg cgttgcatgt
     5281 gtaggagacg atgggaagaa attccaggat cagggataaa tgatccatgg cttctttcag
     5341 ggccttgttg gtcttggtca gttttctcgt gcgttccctg actttgattt ccaggtcttc
     5401 atgcgctttc agcagcgctt cttccgctcg ttttgcctgc gtaatgtctt caagggtttc
     5461 tatcgccccg ataaccgatc ccttggaatt tcggatgacg gcggaagtaa aacgcaacca
     5521 ttttcctctc ttgcccagtt cgggaaaaaa gtccgtcgct tcatatgcac catcaatcaa
     5581 ttttgatttg ataaacttac cggaatacca ttggggaatt ttcttctgat atttatcaac
     5641 cagcagatcc gccatgcacg gcctggccgt tttataaaac gctcgccact gttgtccggt
     5701 gcccatcact tccttcgact tgattccgct taattcttcc agggccctgt tccagtagat
     5761 aatcagatga tctttcccga taataaaggc ggggatcgga gaactcccga taatgctctg
     5821 cagcaattgc ggaccattgc tgaatacttc gccggtccag acgctttcat gacgtccggg
     5881 gcttttattt tttctggacg ggctgcttcc ggttttcatc ttctgctacc gaaaatacga
     5941 ctgacgcgtg cgtttgaact attcaatgat gtattaactc tgttaaagca attatccgcg
     6001 gtgaaggtga cgatttgaaa aatgttcggt atggtttgat tatatagaac gagaaactga
     6061 tgtcaagaaa attaaaaacg aaaaacgctt tacttttgcc gacaatctga atcgtgctgc
     6121 atgcaacaac gtttcagcgc gacgaacgcc gcaccgaccg ctccttcagc atccgcgcca
     6181 gggctttgtt tccgttgaag gcgttcagga ggctgtcttc aaaacgctgc cattttctgt
     6241 ctattttacg ttttgcaaaa aaccggcagt aattgctgac gggaatgaat tcggaattat
     6301 atatattatc gatcagaacc aatcgggcaa tcccggatgc atccatctgg caaagaatat
     6361 tgtagggctt gatgtccatg gttacgatgc ggttctgcag gagatatttt ttcagccggc
     6421 ataatgcatc ggaaaggctg gaaaagtatt tttcggtttg ttcgtctgag gataaatagt
     6481 attctaggct tttggcaacg gtcccgtcat gatccataat cagatcgaaa acagatccct
     6541 gccccagatc ggtctcgaca tctccatgat accgggaaat catctcccag gaaattccat
     6601 gtttttgaag atgccgataa tatttttttt cccgtttaat ttccagaggg ctgtctttta
     6661 ccgtgatttt tatgcagagg ttttgatcct ccggatgttt atagcattcc cgatgcctgc
     6721 cccttccaat caaggttgtc ctgtctatgt aaagcattac tctaccgtta tgatccggat
     6781 gatccgttgc ctgccgctta aaaatccgca gcaacttaat cgaggggcag gaaaaacagg
     6841 caaaaaactc aaatttataa tttcagaaat tttattactc ttccatataa ggttttgcga
     6901 gcaaatccca cgcctcttcc gaatcacatt ggctgtttaa aagataacag ccgagatttt
     6961 ttctttcatt gtttaaccgg acgcttccca gactttcgtt ctcatcaaca tcgagtaatt
     7021 ttttatatcg ttccaatacg ccgtcttccg tgacgagcat aaaacattga tcccgttttt
     7081 tattgtaatg gctcttatat aaatacagat agacattttg atttttctcc acgacgccat
     7141 tgccatacgc tttacgaaat atttttttgc tccgtttttc gcattgatcc tgcaatctgg
     7201 acatctcttt ttgacttttt tctgaagctg gcgttgcttg tatcttttcc gacggggccg
     7261 gttgaggttt tttgagcgtc tctccggaaa ccggcggggc tttttttgtc tcatcaacgg
     7321 ccgaaaccgc aggttttttt gccgctgtgc ttggtacgac ctgtgccctg tctttggcag
     7381 gcaccgattt tcccgttgcc ggggcaccct gatcagccga aatttctttt gacttttcat
     7441 caggcagcag cgccctttga gctgtttcat cgtcagaacg cggcgatggt tctatgacga
     7501 tatcctttga gtccgacgct tgttttatcg cgggggcctg attcatatag atgacccacg
     7561 acataaacga tatcgtcaga ataataacaa gaatggacag aaaaagtttt ttcacggcgt
     7621 tttcctcttt cagatcataa ggttaaaaat ttaaaaccaa caattggtca ggattatatt
     7681 tgtgatattt taatttttac actgaacgaa caatttagga gttgtgtaca tatcagattt
     7741 tcactgattt tcaaaataaa ttttatcatt tttcaaaaac ggccgcgctt cccgtacaga
     7801 ataagattca cgatgccttc ggcggcaatc atttttaccc ggaaagtaaa ttcattgagg
     7861 gagaagcgga ctttaaggat gcttccggac gagatgaagt tattgttcca taagcagaca
     7921 atctttcccg catccggagc acgaacatat attttttcgt tatcggctga tggctaatga
     7981 tccagtgtga tgatgtcctc tatttccact ttgttgagaa gtgcggcttc gatttgattc
     8041 tgaatgtttt gttcaaaccg caggcagact ccgcagtccg agctgatctg cttgggcacg
     8101 ggaatcaatt tgtgcggcaa gccttcctgc ttgagaattt tttccgcctt caaggcatag
     8161 ctcaccgctt taaacagaac aacggcatat gtttttttgt ctttcatggt gtcagcaatc
     8221 gtccggcgcc actcatggtt tccacaatat cgtacatgtt ggaaacggtt cccacggcca
     8281 gcttatcctt gatttcaaaa taattgaggc aggtgccgca aaccagaatc tgaacgcctt
     8341 cgctgttcat ctgtttcaag tcctccagaa catcggaacc ttcaatcgtc aatttcacac
     8401 cggtattgta gaaaatcatc acatccggtt ttttgcccag gtccgcaacg gtatgcacaa
     8461 aggatttgat gagaaccgtt cccagttcat cgtctccccg tcccatttta tcttccgtca
     8521 ccgcaatgac aaaaggcccc gacggagccg cgcctgtttg acaggaagca acaaaatcct
     8581 gatctttatt gaaatcggcc ccccgaccga tatgaatttc ataaatacct ttgccttggg
     8641 attcgacagt cacagcgaag ccgaacttgc cggccagacg ctttacattt tcgagcgcag
     8701 tttcgttatc aacgatggtc gtcaccctgg cgttgtcctc caatgccttt tttgtcagaa
     8761 cgaccggctg aggacaggaa agccctcggg catcaatgat tttaatgtct gccatattat
     8821 tttttcctcc tgcttatttt tcttattgcg tcaatggctt ttgctatttg ctcctgtgtg
     8881 ttgaaatagc tgaagctgaa gcgcaccgtt ccttgaggat aggtgccgat ggttttatga
     8941 gcggatggcg cgcaatggat gccggaacgc gccatgatat taaagcggtc atcgagttcc
     9001 agagcgactg cggccggatc catccc
//
LOCUS       DAONQZ010000084         9021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8402_length_9021_cov_15.664622, whole genome shotgun sequence.
ACCESSION   DAONQZ010000084 DAONQZ010000000
VERSION     DAONQZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8402_length_9021_cov_15.664622"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLR13_10285"
     CDS             complement(<1..652)
                     /locus_tag="PLR13_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQC19769.1"
                     /translation="MSEKYDLIVIGGGPGGLTAAEMAAKKKKRVLIIEKEGWGGTCTH
                     RGCIPTKALLSCSKYYADLKKLKRMGINIADSSVDFPAVKKHQQQIVKVSALGAQKML
                     NDAGAEIKQGVGEIISPSEVRFTDSSGKSENLSTQNILMAWGSIPQVLSGINLSERIL
                     TSDGVLNLNALPSSIIIVGGSVIGAEFATFFAELGTKVTMVEILDTIIPLEDQECAD"
     gene            complement(710..>1432)
                     /locus_tag="PLR13_10290"
     CDS             complement(710..>1432)
                     /locus_tag="PLR13_10290"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQC19770.1"
                     /translation="CPNMAECFASGTATFLIMGNICTRDCLYCNVEHGKPAAIDENEI
                     NHLIDAVQKMKLEYVVITSVTRDDLRDGGAHVFADCIRRLRKTVEHCKIEVLIPDFQG
                     NPSALTKVIDVQPDVINHNMEVVKPMFTRLRPQGNYDVSLKLLKNIGPSSVVSKSGFM
                     IGFGENRDDILRLIDDLAAVSCKRLTIGQYQQPTLKHWPVAKYYHPDEFAEFKEIAYQ
                     KGFQHVESGPLVRSSYHAAKAG"
     assembly_gap    1433..1532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..1728)
                     /locus_tag="PLR13_10295"
     CDS             complement(<1533..1728)
                     /locus_tag="PLR13_10295"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF028191.2"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQC19771.1"
                     /translation="MNPLNEKSSIIRKPDWLKVRPPFSNECRKIRSVLNDLQLHTVCQ
                     EAACPNMAECFASGTATFLIM"
     gene            complement(1803..3245)
                     /gene="gcvPB"
                     /locus_tag="PLR13_10300"
     CDS             complement(1803..3245)
                     /gene="gcvPB"
                     /locus_tag="PLR13_10300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQC19772.1"
                     /translation="MELIFEISKPGRAAAGIPASDVPSINIEQAIGRQYLRDDLDLPE
                     VAEIDLVRHYTNLSRRNFGVDLGFYPLGSCTMKYNPKINENAAALPGFTGLHPYADVK
                     SAQGNLRLLYDMQKYLCAIFGMAEFTLQPAAGAHGELTGVMMIKKYFEKKGQKRKIIL
                     IPDAAHGTNPASSALCGFEIRELKSNGEGGVDIEHLESLMTEDVAALMLTNPNTLGLF
                     ERNIEKVAAIVHGKGGLLYGDGANANAFLGKVRPGDLGFDVIQLNLHKTFSTPHGGGG
                     PGSGPVGVSNALLDFLPVPRIIRKDDKFKWSEKFPDSIGRVRAFYGNFNVIVKAYTYI
                     RSLGADGLKHATEMAVLNANYIKERLKPYFDLPYDRVCMHECVFSGNRQVAQSSVHTS
                     DIAKKLIDYGYHPPTIYFPLIVPEAIMIEPTETERKETLDTFCDAMIAIAREAKENPE
                     GVKNAPVSTPVSRLDEVLAARKPDVCWKKS"
     gene            complement(3256..4581)
                     /gene="gcvPA"
                     /locus_tag="PLR13_10305"
     CDS             complement(3256..4581)
                     /gene="gcvPA"
                     /locus_tag="PLR13_10305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQC19773.1"
                     /translation="MDYCPHTFDDVAQMQSVIGISSLDELFADIPEKFRLQHIPDIPP
                     ALSEMETLSCMGGLAQKNKMPQVTLTGAGAYHHYIPAVVGHIVGRAEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIARLTGTEIANASMYDGASATAEAAVLCAKMSNRNKMIVARSV
                     HPEYRQVLRTYCWANGYTVVEIPYMPSGQIDPDALQKKLDDQTAAVIVQSPNVFGVIE
                     DIAPAVDAAHQKGALLVAGFTEGTSMGLLKPAGAQGADFVVGEGQSFGNPLNYGGPYL
                     GIFAAKEKYMRRIPGRLVGATVDKKGQRGFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAGVYLAALGKNLRKLAELNVYKARYLKNKLLELPGWQDVFSAPVYNEFVLRCPDAA
                     KVNKKLKKEGIIGGYELEKDYPELKDALLFCATEMISKEDMDKVADIVK"
     gene            complement(4599..4997)
                     /gene="gcvH"
                     /locus_tag="PLR13_10310"
     CDS             complement(4599..4997)
                     /gene="gcvH"
                     /locus_tag="PLR13_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQC19774.1"
                     /translation="MSKPNPNDRYYSRDHEWVIYYNDGTAIIGITDYAQEMLTDIVFV
                     DLPEIGRKVVQHATVAVVESVKSVSDVFAPVSGEVIDVNTVLEETPELINQDAFGDGW
                     IVKMRLSKPEELDMLLTANQYDEMLQEDKH"
     gene            complement(5014..6105)
                     /gene="gcvT"
                     /locus_tag="PLR13_10315"
     CDS             complement(5014..6105)
                     /gene="gcvT"
                     /locus_tag="PLR13_10315"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQC19775.1"
                     /translation="MNKTPLYEKHVALKAKIIDFGGWAMPVQYTNVIDEHRTTRNAAG
                     LFDICHMGEILVKGPQALDLLQLVLTRNLADQKIGQVKLSALLNEEGGVIDDLTVYKM
                     DEHDYMLVTNATPKDRDWAWINRIQTDKKFDCALRDISNETGKLDLQGPSSEEILQKI
                     TDADLKNVRFYHFCKAQVAGFDAIVSRSGYTGEDGFEIYAPGNVVGQIWDRLMTEGAK
                     QGMKPCGLGARDTLRLESGMMLNGQDMDESVSPLEVPYGWIVDAQKEFVGSAALRAKQ
                     SSGIRKKLAGLEMTGRGIARHGYKVFCDGKEIGLVTSGTFSPTLNKAIALAFIDIDYC
                     APDTKLEIAIRDTMTAAKVVKLPFYKRLK"
     gene            complement(6607..7167)
                     /gene="def"
                     /locus_tag="PLR13_10320"
     CDS             complement(6607..7167)
                     /gene="def"
                     /locus_tag="PLR13_10320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQC19776.1"
                     /translation="MSTKRVLALWDEAGLNARDTSVLRKKSAELPVPLDKEGREAVQA
                     LVDSFMERDDAVGLAAPQIGINKRIIVFRVKGFEDGQKKIKDIDDIEVLINPRITQER
                     GEKVIGAEGCLSCPDVQVEVSRFPEIKVRALDAKGGKVNKKYLDYIARVVQHEIDHLD
                     GKLIVDYGDIYYPKSKQLFFEKLFKE"
     assembly_gap    7553..7652
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7653..8809
                     /locus_tag="PLR13_10325"
     CDS             <7653..8809
                     /locus_tag="PLR13_10325"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HQC19777.1"
                     /translation="DNNQRLLYIHIPFCEELCPYCSFHRITFKEPLTRRYFQALRREI
                     RIYHEKGYQFEGVYAGGGTPTVLIDELAETLNLSRELFPIRDISVETNPNHLTEKNID
                     VLRQCGVKRLSVGVQTFNDDMLKKIGRYEKYGSGQEIAERLKYYQGHFHTLNADMIFN
                     FPGQTPEMLEADLAVILNLNMDQVTFYPLMISDLTRDIMKKTVGHVDFSNEERFYKLI
                     VRRLEKNYDFSSAWCFSRKESLVDEYVVEFEEYAGLGSGAIGYLYGTCYSNTFDIEGY
                     IASLEKGKLPLQASRKFDLHDRMSYDFLMKLFSTKLDIKAMQKKYDGKFLKTMWKEIA
                     AFEIVRAYRYFDTSLYLTPYGRYLWVIMMREFFIAVNNFRDYCRKQAGVM"
BASE COUNT         2248 a         2259 c         1949 g         2365 t
ORIGIN      
        1 gatcggcgca ttcctgatcc tccagtggaa tgatcgtatc gaggatttcc accatggtca
       61 cttttgtccc caactcggcg aagaaggtgg caaactccgc accgatgaca ctgccgccga
      121 caatgatgat gctcgaagga agcgcattta aattcaaaac gccgtccgat gttaaaatcc
      181 tttcggaaag attaattccc gacagcacct gcggaatgga gccccaggcc atcaagatat
      241 tttgagttga taaattttcg cttttccctg atgaatccgt aaaacgaact tccgaaggag
      301 aaataatttc tccaacacct tgtttgattt ccgcaccggc atcgttgagc attttttgcg
      361 cgcccagagc ggacaccttc acaatctgct gttggtgttt tttgacggcg ggaaaatcga
      421 cggatgaatc cgcgatgttg atccccatcc ttttgagttt tttcagatcc gcgtaatatt
      481 tgctacagga aagcagagct ttggtcggaa tgcagccccg gtgtgtgcag gtcccgcccc
      541 aaccttcttt ttcgatgatc aggactcttt ttttcttttt cgccgccatt tctgcagcag
      601 tcaatccgcc cggtcctccg ccgataacaa tcaaatcata tttttcggac acgtttgtac
      661 ctcgcaattt ataaagacat ttttattaaa gacaaatcag attcaccatt caccctgctt
      721 ttgccgcgtg ataggagctg cgcaccagcg gtccggactc tacatgctga aaaccttttt
      781 gataagcgat ttctttgaac tcggcaaatt cgtccggatg ataatatttg gccaccggcc
      841 agtgtttcag cgtcggctgc tggtattgcc cgatcgtcag gcgtttgcag gaaaccgctg
      901 ccagatcatc aatcaatcgc aaaatatcat cacggttctc gccgaagcca atcataaaac
      961 cgcttttgga tacaacggaa gacggaccga tatttttaag aagcttcagc gaaacatcat
     1021 aattaccctg cggccgcaat cgcgtaaaca tcggtttcac aacttccata ttgtggttga
     1081 taacatcggg ctggacgtca atcacctttg taagtgccga cggattcccc tgaaaatcgg
     1141 gaatcaatac ttctattttg caatgttcaa cagttttgcg cagccgcctg atacaatcag
     1201 caaatacgtg cgcaccgcca tcgcgcaaat catcgcgggt aacggacgtg atcaccacat
     1261 actccagttt cattttctgc acggcatcta ttagatgatt aatttcattt tcgtcaatcg
     1321 ctgcgggttt gccgtgctca acattacagt aaagacaatc gcgtgtgcag atgttgccca
     1381 tgatcagaaa agtcgccgtg ccgcttgcaa aacactccgc catattggga cannnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccatgatc agaaaagtcg ccgtgccgct
     1561 tgcaaaacac tccgccatat tgggacacgc cgcttcctga cagacggtat gcaactgcag
     1621 atcgttcaaa actgatcgta tcttccggca ttcattggaa aacggaggtc gtacttttaa
     1681 ccagtcaggt ttgcggatga ttgacgattt ctcatttaat ggattcatag attattctgc
     1741 atgacgcgtt atcgcacata gaaagattac cccggctcct gagagtcgcc tcgcaatggc
     1801 gcttacgatt ttttccagca gacatccggt ttacgcgcgg ccaagacttc gtcaagccga
     1861 ctcaccgggg ttgatacggg tgcgttcttg acgccttcgg gattttcctt ggcctcgcgg
     1921 gcaatggcaa tcatggcatc gcagaacgta tccagcgtct cttttctttc cgtttcggtc
     1981 ggttcgatca ttatcgcttc cggcacaatc agcgggaagt aaatggtcgg cggatgataa
     2041 ccgtaatcaa tcaacttctt cgcgatgtcg ctggtatgca ccgagctttg agcaacctgt
     2101 ctgttgccgg aaaagacgca ttcatgcatg cagacccggt cataaggcaa atcaaaatac
     2161 ggttttaatc gctccttgat gtagttggca ttgagcacgg ccatttccgt ggcatgtttc
     2221 aaaccgtcag ctcccagtga ccggatatac gtgtaggctt taacaatgac attgaaattt
     2281 ccataaaatg cgcggacacg gccgatactg tccggaaatt tttccgacca tttgaattta
     2341 tcatctttcc gaataatgcg ggggaccggg agaaaatcaa gcagcgcatt gcttacgcct
     2401 accggcccgc tgcccggacc gcctccgccg tgcggtgtgg aaaaggtttt gtgcaggttc
     2461 agttgaatca cgtcgaagcc cagatcgccg ggtctaactt tacctaaaaa agcgttggca
     2521 ttggcgccat cgccgtaaag caggccgcct ttgccgtgaa caatggccgc caccttctcg
     2581 atattgcgtt cgaaaagtcc cagcgtgttg ggattggtca gcatcagggc cgccacatcc
     2641 tccgtcatca gcgattccag atgctcaata tccacaccgc cttcgccgtt ggatttcagt
     2701 tccctgatct caaaaccgca cagagcactc gatgccggat tggttccatg cgcggcatcc
     2761 ggtatcagaa taattttgcg tttctggccc tttttctcaa aatatttttt tatcatcatg
     2821 acgccggtca gctcgccatg cgcgcctgcc gccggctgaa gcgtaaattc cgccatgccg
     2881 aaaatcgcgc acaaatattt ctgcatatcg taaagcagcc gcagattgcc ctgagcggat
     2941 ttgacatcag cataaggatg caatccggta aaaccgggca gagccgcagc attttcatta
     3001 attttgggat tgtatttcat cgtgcaggac cccagaggat aaaatcccag atcgacgccg
     3061 aaattacggc gcgacaaatt ggtataatga cggaccaggt caatttccgc cacttcgggc
     3121 aaatccagat cgtcccgcaa atattgacgg ccgatcgcct gttcaatatt gatggaagga
     3181 acatcgctcg cgggaatacc cgcggcagcg cgtccgggtt tgcttatttc aaaaattaat
     3241 tccattcccc atcccctatt tcacaatgtc agcgacctta tccatatctt ctttggaaat
     3301 catttccgtg gcgcagaaaa gcaatgcgtc tttcagctcc ggatagtcct tctccaattc
     3361 atacccgccg atgattcctt cttttttcag ttttttattt actttcgcgg catcaggaca
     3421 acgcaaaaca aattcattat aaaccggcgc ggaaaaaacg tcttgccagc ctggaagttc
     3481 cagcaattta tttttcagat accgggcttt ataaacgttg agctcggcca gttttctgag
     3541 atttttgccc agagcggcca ggtaaactcc cgctgccagc gcgcacagtg cttcgttgga
     3601 acaaatattg gatgtcgctt tttcgcggcg gatatgctgc tcgcgcgtct gaagagtcaa
     3661 cacgaatccg cgctgacctt ttttatccac ggttgcgccc acaagccttc cgggaatgcg
     3721 gcgcatgtat ttttctttgg cggcaaaaat ccccagatag ggtccgccgt aattcagcgg
     3781 attgccgaag ctctggcctt cgcccacgac aaaatcagcg ccctgggctc ctgcgggctt
     3841 cagaaggccc atagacgtcc cctccgtaaa accggccacc agcaaggctc ccttctgatg
     3901 agcggcatca acagccggcg cgatatcttc gatcacaccg aacacattcg ggctttgcac
     3961 aattaccgcg gccgtctgat catccaattt tttttgcaga gcatccggat caatctggcc
     4021 ggacggcata taagggattt ccacaaccgt atagccgttg gcccaacagt aagtccgcaa
     4081 aacctgccgg tattccggat gcaccgaacg ggcaacgatc attttattac gattggacat
     4141 tttcgcgcaa agcaccgcgg cttcggccgt ggcggaagcg ccgtcgtaca tggatgcatt
     4201 ggcaatttcg gtgccggtaa gacgggcgat catggtctga tattcgtaga tcgcctgaag
     4261 aatgccctgg ctgatttcgg cctgataagg cgtataggcc gtataaaatt ccgcgcgccc
     4321 tacaatatga ccgacgaccg ccgggatata gtgatgatat gcgcccgcgc cggttaacgt
     4381 cacctgcggc attttatttt tttgagccag tccgcccatg catgacagtg tctccatttc
     4441 ggaaagcgcc gggggaatat cgggaatgtg ttgcaatctg aatttttccg ggatatcggc
     4501 aaatagttcg tcgaggcttg agatgccgat gaccgactgc atttgagcaa cgtcatcgaa
     4561 tgtgtgggga cagtaatcca tatcgttttt ttctttcttt aatgtttatc ttcctgcagc
     4621 atttcatcat actgattcgc ggtcagcagc atatccagtt cttcgggttt ggacaggcgc
     4681 attttgacga tccagccatc accgaaagca tcctgattga tcagctccgg cgtttcttcc
     4741 aagaccgtat tgacatcaat cacctctcca ctgacgggcg caaaaacatc ggacacggat
     4801 ttgacggatt cgactacggc cactgttgca tgctggacca cctttcttcc aatttccggc
     4861 aggtcgacaa aaacaatatc ggtgagcatt tcctgagcat aatcggtgat gccgataatg
     4921 gctgtcccgt cattgtagta gatcacccat tcatgatcgc gggaataata tctgtcattc
     4981 gggtttggct tggacataat gccctcctta ttgttatttt aaccgtttat aaaatggcag
     5041 tttaacgacc ttggccgccg tcattgtatc gcgaatcgca atttccagtt ttgtatccgg
     5101 tgcacagtaa tcaatatcga tgaatgccag agcgatagcc ttgttcagcg tgggcgaaaa
     5161 cgtgccggat gtgacaaggc cgatttcttt tccgtcgcag aaaactttat aaccgtgacg
     5221 cgcgatgccg cgtccggtca tttccagtcc agccagcttt tttctgattc cggaactttg
     5281 cttggctctc agagccgcag acccgacaaa ttccttttgc gcatcgacaa tccacccata
     5341 gggaacctcc agcggactga cggactcgtc catgtcctgt ccattgagca tcatgcctga
     5401 ttccagtcgt aacgtgtcac gggcgcccaa cccgcagggt ttcatgccct gttttgcgcc
     5461 ctccgtcatc aacctgtccc atatttggcc tacaacgttt cccggcgcat aaatttcaaa
     5521 tccgtcttca cccgtgtagc cgctgcgcga tacaatcgca tcaaatcccg cgacctgagc
     5581 tttgcaaaaa tgatagaagc gcacattttt caaatcagca tctgttattt tctgcaaaat
     5641 ctcttccgaa gacggcccct gtaaatccag cttacccgtt tcattgctga tatctcgcaa
     5701 tgcgcaatca aactttttat ccgtctgaat tctgttgatc caggcccagt ctctgtcttt
     5761 cggcgtagcg ttggtgacga gcatatagtc atgctcatcc attttataaa cggttaagtc
     5821 atcgataacg ccaccttctt cattgagcag cgcggataat ttaacctgtc cgattttctg
     5881 atcagccaga tttctggtca aaaccaattg cagaagatca agcgcctgcg gtcctttgac
     5941 cagtatttct cccatatggc aaatatcaaa aagtccggca gcgtttctgg ttgttctgtg
     6001 ttcgtcgatg acgttggtat actggacggg catggcccag ccgccgaaat caataatttt
     6061 ggccttcagg gcgacatgct tttcgtataa tggcgtttta ttcaattttg attcatccaa
     6121 tctttaaaaa atatacggca atcactcagg taaaaagcat gcccgtgaaa actataattt
     6181 ccggaaaaaa atatccctga aacaacccga ccgcatgtca gattccggga atctttcatc
     6241 cttgcaaaaa ggataataca atacttcaaa taccggattt agagcataaa accggcttgt
     6301 ccggaaaaaa tcatttcgca ggggaatata tccattgcac ctgtgatgat tgctttttaa
     6361 aaattactat aagtaaaaat atatttcaat aatgaatact gaaataatct tgtatcaatc
     6421 ccgtgtcgca gcagatgccc ttcagagcac tccactcccg ggatcgcgac ttacattccg
     6481 agtataactg cattcgctct caacgaatct taggatcagc gagtgtcatc ccatttcgct
     6541 gcacttcggg gataattcca ctaacgcttc gagctgcgct acgcttgctc tcagcgagtg
     6601 tcattattat tctttaaaca gtttttcaaa aaacagttgc ttgctcttgg gataataaat
     6661 gtcgccataa tcaacgatca atttcccgtc aagatgatca atctcgtgtt ggacaacacg
     6721 agcgatataa tccagatatt tcttgttgac tttgcctccc ttggcatcca aggcacgtac
     6781 ttttatttca ggaaatcgac tgacctcgac ctgcacatcc ggacaggaca gacacccttc
     6841 cgccccgatg accttttctc cgcgttcctg ggtaatccgc gggttgatga gaacttcgat
     6901 atcatcgata tcttttatct ttttttgacc atcctcaaag cctttgaccc ggaaaacgat
     6961 tattctttta ttaataccga tttgaggcgc ggccagaccc accgcatcat ctctttccat
     7021 aaaagagtct accaatgcct gaacggcttc ccgtccttcc ttatccaggg gcacgggcaa
     7081 ctcagcggat tttttcctta aaacagaagt gtcgcgggcg tttaggcctg cttcatccca
     7141 taatgctaaa actcttttcg tagacatgat acttgctcaa aaattatttt gaaaaacact
     7201 tatcacctat tccccgttat ttcaagtcat gagtcccgac cgttagaagc aaataaccca
     7261 aaggtcacta taggcgcact gaaatttgac ggacgaatta tccgatcccg atttatcggg
     7321 attggaaatt atcccgcgtg cgaagctaca tggagataat tgtcgcttgc aatcaacgca
     7381 catattttgt taaacaacat aaaaccctga ttttataaaa aaactgattg aggggattta
     7441 catttgatta atcaaatcgt taccagaatt gccaagcggg agttcgcccg ttcactgaaa
     7501 tttgaagatg gaagttcgcc ggaactgcct tcctgcgccg acaataacca gcnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgacaat aaccagcgcc ttctctacat
     7681 tcatatcccg ttttgtgagg agctctgtcc ctattgctcc tttcaccgga ttaccttcaa
     7741 agaaccttta acacgcagat attttcaggc cctgcgccgg gaaatcagga tttatcatga
     7801 aaaaggctat caattcgaag gggtttatgc gggcggcggc accccgaccg tgctgattga
     7861 tgaactggcg gaaaccttga atctttcgcg ggaactcttt cccatccggg atatatccgt
     7921 ggaaacgaat cccaaccacc tgaccgaaaa aaatatcgac gttctgcgac aatgcggcgt
     7981 caagagactt tccgtgggcg tgcaaacctt caacgacgat atgctcaaaa aaatcggacg
     8041 ctacgaaaaa tacggctccg gtcaggaaat cgccgaacgg ctgaaatatt atcagggaca
     8101 ctttcacact ctaaacgccg acatgatttt taatttcccc ggacagacgc cggagatgct
     8161 cgaggcggat ttggccgtta ttcttaattt aaacatggat caggtgacct tttatccctt
     8221 gatgatttcc gacctgacgc gggacatcat gaaaaaaacg gtgggccatg tcgatttcag
     8281 caacgaagaa cgtttttata aattgatcgt ccggcgtctg gagaaaaact atgatttttc
     8341 atcggcatgg tgcttctcgc ggaaggaatc gctggttgac gagtacgttg tggagtttga
     8401 agaatacgcg ggactcggca gcggcgccat cggctatctt tacggaacat gttattccaa
     8461 cacgtttgat attgaaggat acatcgcttc tctggaaaaa gggaaattgc cattgcaggc
     8521 ttcccgcaag ttcgaccttc atgatcggat gagctatgat tttttaatga aactattcag
     8581 cacgaagctg gatatcaaag ccatgcagaa aaaatacgac gggaaatttt taaaaaccat
     8641 gtggaaggaa atagcggctt ttgaaatcgt cagagcgtat cgttattttg atacctctct
     8701 gtatttgacg ccgtatggcc gttacctgtg ggtcattatg atgcgggaat ttttcattgc
     8761 cgtgaacaat ttccgcgatt attgccgcaa gcaggcagga gtgatgtgat attttgtgtc
     8821 acttcaagcg tatgagaaat caaaaaaaca agatgtctcc ccatgacaaa aatgaatcga
     8881 tagagcaaac tcgcatcaaa agaggataac gatggatgaa caacaaaaag aattgttgaa
     8941 gcaattgccg aagattgacg aaattattct tcttctggag aaacaaaaca tctatgatct
     9001 ggcgccgcac gaaatcgtca a
//
LOCUS       DAONQZ010000085         8926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8531_length_8926_cov_17.589900, whole genome shotgun sequence.
ACCESSION   DAONQZ010000085 DAONQZ010000000
VERSION     DAONQZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8531_length_8926_cov_17.589900"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>502)
                     /locus_tag="PLR13_10330"
     CDS             complement(<1..>502)
                     /locus_tag="PLR13_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HQC19778.1"
                     /translation="ENAGWAYLVGAAAAIKKGQKVAADIAETLGEGLQSFCIPGSVAD
                     DRQVGIGHGNLASMLLREETKCFAFCAGHESFAAAEGAIGLAKSANKARKVPLRVILN
                     GLGKDAAHIIARVNGFTHVATAFDYKTGKVSVAKEKAYSKGDRAAVRCYGADDVREGV
                     AIMHLEG"
     assembly_gap    503..621
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<622..1300)
                     /locus_tag="PLR13_10335"
     CDS             complement(<622..1300)
                     /locus_tag="PLR13_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19779.1"
                     /translation="MEYTHEVEQMCVVAKGAKNGPAPIPQEGKWTMVKEIKDISGLTH
                     GVGWCAPQQGTCKLTLNVKDGIIEEALVECLGCTGMTHSAAMAAEILPNKTILEALNS
                     DLVCDAINTAMREIFLQIVYGRSQTAFSEGGLPIGAGLEDLGKGQRSVIATMYGTKKK
                     GPRYLEMAEGYVLEIALDSDNEIIGYKFVNLGVMMDNIVKGMDPAAALAKATGTYGRF
                     AEAVKVIN"
     gene            complement(1591..2340)
                     /locus_tag="PLR13_10340"
     CDS             complement(1591..2340)
                     /locus_tag="PLR13_10340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19780.1"
                     /translation="MDIPRIFNITESAHRIHNPITPEKLAILGSALRLESGDRVLDLG
                     SGSGEMLCTWARDHGIIGTGIDMSRLFTEQAKRRAEELGVADQVTFIHGDAAGFVSGE
                     KVDVAACLGATWIGGGVAGTIELLAQSLKLGGIILIGEPYWRQLPPTEDVAKGCLAHT
                     IADFIMLPELLASFDRLGYDVVEMILADQDGWDRYEAAKWLTMRRWLEANPGDELAKE
                     VRAQLTSEPVRHAAYTREYLGWGVFALMSRR"
     gene            2586..3173
                     /locus_tag="PLR13_10345"
     CDS             2586..3173
                     /locus_tag="PLR13_10345"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HQC19781.1"
                     /translation="MDRKFYHRDTLRVARELLGKKLVRRINGLELSGMIAETEAYCGR
                     ADSACHAHRGKTQRNAVMFGKPGHAYVYFTYGMHYMLNLVTEEEENPCAVLIRAILPI
                     DGIAAMEARRKKKGTELTNGPAKLCQALSIDKSLNGWNVTLGSELWVENNKKIPDSKI
                     ITTPRIGIDYAKTKDREALWRFILKKDDLEGRSVR"
     gene            complement(3177..4253)
                     /locus_tag="PLR13_10350"
     CDS             complement(3177..4253)
                     /locus_tag="PLR13_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQC19782.1"
                     /translation="MNMNKKPGYNFQNIGFIVLLVIITLFFFYIVKPFFFAVFWAALI
                     AGIFMPLHRALNTKITNPNLCAGATLVVILLCLIIPIAFLFNLLVLEAFDIYKSFDSQ
                     GGAWMKTLTATLNTLSQSALFAKLNLDQAFILGKFQDIIKAVSGYVINHISNFTQNTI
                     LVIVKVAVMIYTLFYFLRDGERLLESMVGHIPVNKRYLDTFIYQFLSTAKASLKFTFV
                     IGGIQGFLGGLVFYITGIERALIWGVIMMGLSILPGVGCAFVWVPAGIIMLVLGHTWQ
                     GIVILIFGSVIISSVDNLLRPVLLGRDTNMHALLIFLSTLGGLAVLGLSGFVMGPIIA
                     ALFLASWKLFLEFYQEKQEEKIES"
     gene            4593..4793
                     /locus_tag="PLR13_10355"
     CDS             4593..4793
                     /locus_tag="PLR13_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19783.1"
                     /translation="MALDEPAKDDEVFDENGTKFVIDKDIFNQAKPINVDFIETPQGS
                     GFKVTSNLSAAGGGCGSSCSGC"
     gene            4835..5773
                     /locus_tag="PLR13_10360"
     CDS             4835..5773
                     /locus_tag="PLR13_10360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19784.1"
                     /translation="MTDEKSTKRDLAEKLLQKGKYDEAIVLLEDIHQESPDEDPVLLM
                     LAWAYYDSGKTGQAEKCLTILFDRELERKVFTGFAFDELVRLYKQKKDFPNLVNICER
                     AVAAQPDDVGLLTELGNAYLQAGQADRARLTYEKLIAMEDDNPAFYCLWGEALFAAGL
                     NKESEDAYRKAGQLDPDQLDSYYFRIAVLFQKADNHQEARRLLNKCLNLNPSSPLYYC
                     SLGDSLIGLGKLQDAINAYNQAVEFDRADADAYYNRLGNSLMKTGNFSEAVAAFQTAI
                     RLEPLPIYYLNLSSAYRKMGLDHEADQTIGKADKIH"
     gene            complement(5775..6272)
                     /locus_tag="PLR13_10365"
     CDS             complement(5775..6272)
                     /locus_tag="PLR13_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19785.1"
                     /translation="MAPESVEEEIDYSGRPFDYRDMDTSSAMICENDPAIRQKLRENL
                     QTLNFNVIEPMTAKEALKYTTFQTFNVIVVNEHYDTDKDGVNQVLKYLETLPMSVRRQ
                     VFVVLISSTFATMDYMNTLNRSVNLIIHMEEISEIGLILKKEMEENEYFYHVFKELQR
                     KLGRV"
     gene            complement(6325..7509)
                     /locus_tag="PLR13_10370"
     CDS             complement(6325..7509)
                     /locus_tag="PLR13_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQC19786.1"
                     /translation="MRKPEIDHILTKMLESYPNISDFIITVGKPFQVEAAGQLVPVPL
                     RPHFEKLTPFQTEIFALNLVNQDHRLMTDLLKEGACDLSYELTGVARFRVNIFSQMGN
                     YSIVLRKLESLVPSCADLGLPDAYYRISREKNGIVLVTGATGTGKTTSLAAIINEINE
                     TSSVHIITLEDPVEYTHPQKKATINQRELGKDFDTFSSGLRAAMRQAPKVILVGEMRD
                     RESVEIALSAAETGHLVLSTLHTVDAGQTINRIIGMFPRDEENQIRVRLADAIRWIAC
                     QRLLPKIGGGRVAAFETLSTSLRVKDVILHGESEGKTFNDIITQGRPFGMMTFDDCII
                     DHFSKDLITEETAIAFATNRAVVNRGIDAVKKARGDQAVNIGTLEVDRSYGKAKMDTW
                     KR"
     gene            complement(7531..8613)
                     /locus_tag="PLR13_10375"
     CDS             complement(7531..8613)
                     /locus_tag="PLR13_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQC19787.1"
                     /translation="MAKIDAFFNLMHDQGASDLHLVAGQQPAIRIRGDIERIKYDVLT
                     NDALKVLLYEITPEHKIKQFEETGDVDFAYEVPNLARYRVNFFRQKNGMAAVFREIPD
                     KIQTCQQLGLPNVIAKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRLRRDHIITIE
                     DPIEFVHKSQNCIVNHREVGVHTKTFTAALRGALREDPDIILVGEMRDLETISLAIEA
                     ASTGHLVFSTVHTTSAPKTVDRIIEVFPASEQLQIRSTLADGLRAVISQVLFKRIDVK
                     SRCVALEICIATPAVRNLIRESKTYQIPSMMQTGKKYGMQLLDDAIMDLYNRGWISGD
                     DAYIKANEKARFRPLLRQPPTDFTEA"
     gene            8851..8926
                     /locus_tag="PLR13_10380"
     tRNA            8851..8926
                     /locus_tag="PLR13_10380"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8884..8886,aa:Ala,seq:cgc)
BASE COUNT         2242 a         2196 c         2151 g         2218 t
ORIGIN      
        1 gccttccaga tgcatgatgg ccacgccttc gcgcacgtca tcggcgccgt agcagcgaac
       61 tgccgcgcgg tcgcccttgg aataggcttt ctccttggcc acagagactt ttcctgtttt
      121 ataatcgaat gccgtcgcga catgggtgaa tccgttgacg cgggcgataa tgtgagccgc
      181 gtcttttccc aatccgttca gaatgacgcg cagaggaacc ttgcgcgcct tgttggccga
      241 tttggcaagt ccgatcgcgc cttccgctgc cgcgaatgat tcatgacctg cgcaaaacgc
      301 aaagcatttg gtttcttccc gaagcagcat ggaagcgaga ttgccgtgac cgatgcccac
      361 ctgacggtca tcggcaaccg agccgggaat gcagaaggac tgcaatcctt cacccagtgt
      421 ttcggcgata tcggcggcta ccttctgacc ttttttaatg gcggcggcag cgccgaccag
      481 ataagcccag ccggcgtttt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn ngattgataa cttttacagc ttcagcaaaa cggccgtaag
      661 ttccggttgc tttcgccaga gcggcggccg ggtccatgcc cttgacgatg ttgtccatca
      721 tcacgcccag attaacgaat ttgtatccga tgatttcatt gtcactatcc agggcgattt
      781 ccagcacgta cccttcggcc atttccagat aacggggacc tttctttttg gtaccgtaca
      841 ttgtggcgat cacgctgcgc tgtcccttgc ccagatcctc gagtccggct ccgatcggca
      901 gaccgccttc ggagaaagcc gtctgtgagc ggccgtaaac aatctgcaga aaaatttccc
      961 tcatcgcggt gttgatcgca tcgcagacaa ggtcggaatt cagcgcttcc agaatggttt
     1021 tgttcggcag aatctcggca gccattgcgg ccgagtgggt catgccggtg caaccgagac
     1081 attcgacgag tgcctcttca atgatcccgt ctttgacgtt cagggtcaac ttgcatgttc
     1141 cctgctgcgg cgcacaccag ccgacaccat gggtaagacc gctgatatcc ttgatttctt
     1201 tcaccatggt ccatttgccc tcctgtggga tcggggcggg accgtttttc gcgcctttgg
     1261 caactacgca catctgttca acttcatgag tgtattccat aaactatctc tccttccggt
     1321 aataccagtt cgctttcacg gttcatttca gccgttgaaa gtaatttgtt tttataatac
     1381 tgccggtcac aatctagcac cggaggggtt atttttcaaa ttaatttgtt gtaaggaagc
     1441 ttaagttttt gattatattg agatgttaaa aaaaagattt tctcaagaga gaacgaaaag
     1501 caaagccgac gccgcaaagc ggcgtcggct tgaatgcgtt atcgggtatg attggatttg
     1561 cccctctttc tgaatcgccc ggtaatctcc tcatcgccgc gacatcagcg caaacactcc
     1621 ccagcccaaa tattcacgcg tgtaagcggc gtggcgcacg ggctccgagg tcagttgggc
     1681 acgaacctct ttggccaatt cgtcgccggg attggcttca agccatcggc gcatggtgag
     1741 ccatttggcc gcctcgtatc ggtcccaacc gtcctgatca gccagaatca tttcaacgac
     1801 gtcgtagccg agacggtcga aagacgcgag aagttccgga agcatgataa agtcggcaat
     1861 cgtgtgggca agacaccctt tggcaacatc ttccgtcggc ggtaactgcc gccagtaagg
     1921 ctcgccgata aggatgatcc ctccgagctt caggctttgc gccagaagct cgatcgtgcc
     1981 ggcgactccc ccgccgatcc acgtggcccc tagacaggct gccacatcga ccttctcgcc
     2041 agaaacaaag cccgcagcat cgccgtgaat gaacgtgacc tgatcggcga cgccgagttc
     2101 ttcagcgcga cgttttgctt gctcggtgaa caaccggctc atgtcgatgc cggtgccgat
     2161 gatgccgtga tcgcgtgccc aggtgcacag catctccccc gaaccgctgc cgaggtcaag
     2221 tacccggtcc cccgattcca gacgcagcgc cgagccgaga atggcgagct tttcgggtgt
     2281 aatcgggttg tggatgcggt gagcgctctc agtaatgttg aagatccggg gaatatccaa
     2341 tgcagaaaat ctcctttcgg gtgtgaatag attcggcgtg ggccgaacca ttaatatatc
     2401 aattttgttt ttccgattct tgaatattaa cctacaccat ctattgataa aagaaaatta
     2461 aaatatcctt gaaaatcaat ttcaaataaa cttttttcac ttggcctttt gtgatagtaa
     2521 catccttacg tcacgttggt tatttattgg taaatatgtc cgaagaacaa ctcattatga
     2581 aaatcttgga tagaaaattt tatcatcggg atacgctgcg ggtggcgcgg gagcttttgg
     2641 ggaagaagct ggtgcggcgg ataaacggtc ttgaactttc cggcatgatt gcggagacgg
     2701 aggcttactg cggccgggca gacagcgcct gccatgccca ccgcggcaag acacagcgca
     2761 atgcggtcat gttcggcaaa cccggccatg cctatgttta ttttacctat ggtatgcact
     2821 acatgctcaa tttggtcact gaagaagagg aaaatccctg cgccgttttg atccgcgcaa
     2881 ttctaccgat tgacggcatc gcggcgatgg aagcccgccg caaaaagaaa ggcaccgaat
     2941 taaccaacgg accggccaag ctttgccagg cgctgtccat cgacaaatct ttaaacggct
     3001 ggaatgtaac gctgggcagt gaattgtggg tggaaaataa taaaaaaatc ccggattcta
     3061 aaatcatcac gacaccccgc atcggcatcg attacgcaaa gacaaaagac agggaggcgc
     3121 tttggcgatt cattttgaag aaagacgatc ttgaaggaag atccgtcaga taaaaatcag
     3181 ctttcaattt tttcctcttg tttttcctga taaaactcaa gaaataattt ccagctggcc
     3241 aggaaaagag cggcgatgat cggtcccatg acaaagcccg acagtcctaa aacggccaac
     3301 ccgcccagcg tggaaaggaa tatcaaaagc gcgtgcatgt tcgtgtcgcg gcccagcaaa
     3361 accggccgca ataaattatc cacactgctg ataatcaccg atccgaaaat aaggatgaca
     3421 atgccctgcc aggtatgccc caaaaccagc atgatgattc cggcgggcac ccagacaaag
     3481 gcacatccca ctcccggcaa tatggatagc cccatcatga tcacgcccca gatgagagcc
     3541 ctctcaattc ccgtaatgta aaaaacaagg ccgcccagga atccctggat gccgccgatg
     3601 acaaacgtaa atttcaggga cgctttggcc gtggaaagga actggtaaat gaacgtatcg
     3661 aggtagcgct tattcacggg aatatgaccg accatagatt ccagaagtct ttccccgtcg
     3721 cgcaggaaat aaaaaagggt gtagatcatc acggcaactt tgacgatgac aagaatggtg
     3781 ttttgcgtaa aattcgaaat atgattgatg acgtatccgc tgaccgcctt gatgatgtcc
     3841 tgaaattttc ccagtataaa agcctgatcc aggttcaatt tggcaaacaa agcactctgg
     3901 ctcaatgtgt tgagcgttgc cgtaagtgtt ttcatccagg cgccgccctg ggaatcgaag
     3961 gacttataga tatcaaacgc ttccagcaca agaaggttaa acagaaatgc gatagggata
     4021 atcaaacaaa gcagaataac aacgagagtc gcgcccgcgc ataaattggg atttgtgatt
     4081 tttgtattca acgcccggtg caggggcata aaaatgccgg cgatcagcgc cgcccagaaa
     4141 accgcaaaga aaaaaggctt aacaatgtaa aagaagaaaa gggtgatgat gacgagtaaa
     4201 acgataaaac cgatgttttg gaagttataa ccgggctttt tgttcatatt catccatcga
     4261 cctatcatcg cgaaccctga tgcgtctata ttgccatcag actgtttttg ctgcggaagg
     4321 tataaaaacg taattaataa taaactacag caacttataa tgtcaattca taaataaccc
     4381 ttggcaagaa aaagattttg attgacatat tgtcaggtat gactatttta catccgagat
     4441 gaataacgac tattcatcaa aaaggaggat atttgatgtt tacagtgaca gagaaggcat
     4501 tggaggtaat caaagatttt atcaaagcaa aaaaacctga ttccgctgtg cggataacaa
     4561 tgtctattgg ctgatccggc cccgcactgg ggatggctct ggatgagccg gcaaaagatg
     4621 atgaagtatt tgatgaaaac ggaacaaaat ttgtaattga caaggacatt tttaatcaag
     4681 cgaaaccgat taatgttgat tttatagaaa caccgcaggg ctctggattt aaggtaacgt
     4741 ccaacctgtc tgccgccgga ggcggttgcg gttcttcctg cagcggctgc tagtctttgg
     4801 taaggggagg agacatcctc cccttttttt aacgatgacc gacgaaaaaa gcacaaaaag
     4861 agatctggcg gaaaagctcc ttcaaaaagg caaatatgat gaagccattg tcctgctcga
     4921 ggatattcat caggaatcac cggacgaaga ccctgtcctt ttaatgctgg cctgggcata
     4981 ttacgacagc ggcaaaaccg gtcaggccga aaaatgttta acgatattgt tcgaccgcga
     5041 gctcgagaga aaggtgttta ccggctttgc gttcgatgaa ctggtgcgtc tttacaagca
     5101 gaagaaggac ttcccgaatc tggtaaatat ttgcgagagg gcagttgccg cgcagccgga
     5161 cgatgttggg ctcctgacgg agttaggcaa tgcctatctt caggcgggac aggccgatcg
     5221 ggcacgttta acgtatgaaa aactgattgc catggaagac gacaatcccg ctttttattg
     5281 tttgtggggc gaagcgttgt ttgcggcggg attaaacaaa gaaagcgaag acgcttaccg
     5341 gaaggccggc caactggatc ccgatcagct cgacagctac tatttcagga ttgccgtttt
     5401 attccaaaaa gccgataatc atcaggaagc ccggcgtctg ttgaataaat gcctgaacct
     5461 caatccttcc agtcccctgt attactgctc gctcggggac agtcttatcg gtctgggaaa
     5521 attacaggat gcgatcaacg cttacaatca ggctgtcgaa tttgatcggg cagatgcgga
     5581 tgcctactac aaccgattag gtaactctct gatgaaaacc ggaaattttt cggaggcggt
     5641 agccgcgttt caaacagcca tacggctcga gccgctgccc atctattatc tgaacctgtc
     5701 ttccgcctac agaaaaatgg ggcttgatca cgaggccgat cagacaatcg gcaaagccga
     5761 taaaatccac tgaattaaac tctgcccagt ttgcgctgca actccttgaa aacgtgatag
     5821 aaatattcgt tttcttccat ttcttttttc agaatcaggc ctatttccga aatttcttcc
     5881 atatgaatga ttaaattcac gctcctgttc aacgtgttca tgtaatccat cgtggcaaac
     5941 gtcgagctga tcaggacaac gaaaacctgc cggcggacgg acatgggaag agtctccaga
     6001 tatttaagga cctggttgac gccgtccttg tccgtgtcat aatgttcatt gacaacaatc
     6061 acgttgaacg tctgaaacgt cgtgtacttc agagcttctt tcgccgtcat cggttcaatc
     6121 acgttaaagt taagcgtttg cagattttct ctcagctttt gcctgatcgc cggatcgttc
     6181 tcgcagatca tggccgagct ggtatccata tcccggtagt caaaaggacg gcccgagtaa
     6241 tcgatctcct cttcgactga ttctggtgcc aatttatccg tgttcataat ccattcccga
     6301 tagttatttt attaagtccg gacattatct tttccatgtg tccatttttg cttttccgta
     6361 agaccggtcg acttccagcg ttccgatatt gacggcttga tcgccgcggg ctttcttgac
     6421 ggcatcgata ccgcgattga ctacagctct gttcgtcgca aacgcaatgg ccgtttcctc
     6481 cgtaatcaaa tccttgctga aatgatcgat aatgcagtcg tcaaaagtca tcatgccgaa
     6541 aggccttccc tgtgtgatga tatcattaaa tgtttttcct tccgattcgc cgtgcagaat
     6601 aacatccttc acccgcaggc tggtgctcag tgtttcaaat gcggcaacac gccccccgcc
     6661 tattttcggc agtaaacgct gacaggcgat ccagcggatg gcatcggcca gccgcacacg
     6721 tatctgattt tcttcatccc gcgggaacat accgataata cggttgatcg tctgtcccgc
     6781 atccacggtg tgcagtgtac tcaatacgag atgaccggtt tccgcggcgc ttaaagcgat
     6841 ttcgaccgat tcccggtcgc gcatttcacc gaccagaatc accttgggag cctggcgcat
     6901 cgcggcccgt aagccgctgg aaaatgtatc gaaatccttg ccgagttccc gctgattgat
     6961 tgtagccttc ttctgtggat gcgtgtattc caccgggtct tccagggtaa taatgtggac
     7021 ggaactggtt tcattgattt cgttgatgat cgcggccagt gacgtggttt taccggtgcc
     7081 ggtcgctccg gtgactaaca caatgccgtt tttctcgcgg gatatcctgt aataggcatc
     7141 cggcaatcct agatcggcgc aggaaggaac aagggattcc agctttctta agacaatcga
     7201 ataattgccc atttgcgaaa aaatattcac gcgaaaacgc gccacgccgg tcaattcata
     7261 cgacaaatcg catgcgcctt ccttaagcaa gtccgtcatg agacggtgat cctgattcac
     7321 caggttcaaa gcaaaaattt ccgtctgaaa cggcgtcagt ttctcaaaat gcggcctcag
     7381 cggaacggga accagctgac cggcagcctc cacctgaaaa ggtttgccca cggttatgat
     7441 aaaatcagaa atgttgggat acgactccag cattttggtc agaatatgat caatttccgg
     7501 ctttctcata tacgatcctt tctcgataca ttacgcttcg gtaaaatcgg tcggcggttg
     7561 tctgagcagc ggacggaacc gggctttttc attggctttg atataggcat catcgccgct
     7621 tatccaaccg cggttgtaca gatccataat ggcatcgtcc agaagctgca tcccgtattt
     7681 tttaccggtc tgcatcatgg agggaatctg gtaggtcttg gattcccgga ttaagttgcg
     7741 gaccgccggc gtggcaatac agatttccag agcgacgcag cggcttttga catcaattct
     7801 cttgaataaa acctgagaaa taacggcgcg taaaccatca gccagtgtcg agcggatctg
     7861 caactgttcg ctggcgggaa aaacttcgat gattctgtca acggttttgg gcgcgctcgt
     7921 cgtatgaacg gtggaaaaaa ccagatggcc ggtggaggcc gcttcaatcg ccagagaaat
     7981 ggtttccaga tcgcgcattt ctccgaccag aataatatcc ggatcctcgc gtaatgctcc
     8041 ccgtaatgcc gcagtaaaag ttttggtatg cacccccact tcgcggtggt tgacgatgca
     8101 attttggctc ttatggacaa attcgatcgg gtcttcaatg gtaatgatat ggtcccggcg
     8161 caggcggttg gcttcatcaa taatagcggc cagcgttgtg gacttgccgc tgccggtcgg
     8221 ccctgtcacc agtacaaggc cccgcggaag ctgggccagt ttggcaatta cgttgggcag
     8281 tcccagttgc tgacaggtct gaattttatc gggaatttct cgaaaaaccg ctgccatgcc
     8341 gtttttctgc ctgaaaaaat tgacacgata acgggccaga ttgggcactt cataggcaaa
     8401 gtcgacatcg ccggtttctt cgaactgttt gattttgtgt tccggagtaa tttcataaag
     8461 aagcactttc aaggcatcat tggtaagaac atcatactta atccgctcga tatcgccgcg
     8521 aattctgatt gccggctgct gtccggcgac aagatgaagg tcggatgcgc cctgatcgtg
     8581 cattaaatta aagaaagcgt caattttggc catctatttg ctcctcgagg tgatttgatt
     8641 ttccttatca ttttttcttt gtgaaaagaa tatcttattt gaaaatttca cattcatcga
     8701 taaaaacata tttcattttt ttatgtgcgt agagtataag aaaaaccctg ctgttttgtc
     8761 aattcaataa tcatagcaac tatggcttga caccgcgatc atcaataata tatgcttccg
     8821 tgcaattacc cggcgggaat tcttaaggtt ggggatgtag ctcagctggg agagcgcgac
     8881 gttcgcaatg tcgaggccag gggttcgatc cccctcatct ccacca
//
LOCUS       DAONQZ010000086         8862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8612_length_8862_cov_16.178608, whole genome shotgun sequence.
ACCESSION   DAONQZ010000086 DAONQZ010000000
VERSION     DAONQZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8612_length_8862_cov_16.178608"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..>1382
                     /locus_tag="PLR13_10385"
     CDS             289..>1382
                     /locus_tag="PLR13_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQC19788.1"
                     /translation="MKKVCLLLFLFFIHITLTACDTKQESVNSRPPEEKPPAYGDILV
                     RGDIGDASNLIPILASDSASHNIAGMIYNGLVKYDKNMNIVGDLAESWDISSNGLVIT
                     FHLRKGIKWHDGKPFTSADVLYTYQATIDPKTPTAYAGDFLKVKKVEAPDDYTFRVTY
                     DKPFAPALISWSSAILPRHLLAGKDITQSSLSRHPIGTGPYKFKEWVAGQKIVLVSNP
                     DYFEGQPYIDGRMTRIIPDTATMFLELRAQNIGMMGLTPLQYTRQTENNLFKNNFNKY
                     RYLSFAYTFMGYNLKNPLFADKRVRQAISYAINKDEIISGVLLKLGKPASGPYKPGTW
                     AFNDKVKVYNYNPQKALALLNEAGWKKTGS"
     assembly_gap    1383..1482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1483..1684
                     /locus_tag="PLR13_10390"
     CDS             <1483..1684
                     /locus_tag="PLR13_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HQC19789.1"
                     /translation="KERKKYYDRFQEILAEDQPYTFLYVPEALVIINKRFRGIEPAPI
                     GLEHNFIKWYVPKDEQKYTLTR"
     gene            complement(1739..2530)
                     /locus_tag="PLR13_10395"
     CDS             complement(1739..2530)
                     /locus_tag="PLR13_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQC19790.1"
                     /translation="MQNNNDQQPRRIKIGLFGFGNTGRMVADEFYRDGLFDLEWVVRR
                     THKDNHKYATRLLGYEADDAPIFSVDEVSPEFFSHRPVDIIVDFSSARAHNSYAPAAD
                     VGIKIISAISKYGGKDMEELKQLSAKTAVLYSPNITLGINFLLVASQILQKVAPHADI
                     EIVEEHFRSKKEQSGTAVKIANILGLEKDKHVNSIRVGGIVGKHEIIFGLPNQTIRLI
                     HESISKRAFGQGAIFVSKWLIDAPIGLYSMENIIIDMFKKNIPVY"
     gene            complement(2844..2929)
                     /locus_tag="PLR13_10400"
     tRNA            complement(2844..2929)
                     /locus_tag="PLR13_10400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2893..2895),aa:Leu,seq:gag)
     gene            complement(3026..3403)
                     /gene="secG"
                     /locus_tag="PLR13_10405"
     CDS             complement(3026..3403)
                     /gene="secG"
                     /locus_tag="PLR13_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQC19791.1"
                     /translation="MYTLLIALHIVICLVLILIVLLQAGKGANMGAAFGGSSQTVFGS
                     SGAGTFLGKLTAGVAVVFMLTSLILTYMANHRSTSSLMDQRPPAVDRTMPAPGQDATA
                     LPSPVPGPDAAKMPAVPPAPASK"
     gene            complement(3515..>3967)
                     /locus_tag="PLR13_10410"
     CDS             complement(3515..>3967)
                     /locus_tag="PLR13_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19792.1"
                     /translation="TPGHTPGHLSFLIPDEEILFLGDYDLTTFGPWYGDLYSSIEQTI
                     QSIRRLKSISARRWIASHNTGLFEENPGKLWDDYENVIYQREEKILNFLKEPKSLDEI
                     AGAWLIYGRPREPREFFESNERALVGKHIEYLERIGRIAIDEGKYVRL"
     assembly_gap    3968..4067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4409..4795)
                     /locus_tag="PLR13_10415"
     CDS             complement(4409..4795)
                     /locus_tag="PLR13_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19793.1"
                     /translation="MEHFVKVFSDGETLIAAAVGELNEAAYLLMRKEIVDSIKTCNAK
                     RILIDIRNAIVHASVMEIYQFAESSLKMFPLGFRYAVVYSEKTMSEEDAKFGETVACN
                     RGGHVQVFRDVSKAKKWLAIAEVEHA"
     gene            complement(5976..6179)
                     /locus_tag="PLR13_10420"
     CDS             complement(5976..6179)
                     /locus_tag="PLR13_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQC19794.1"
                     /translation="MSEGKVKWFNEKKGFGFIANNDGGDVFVHFNDIQASGFKTLQEG
                     ASVSFDIENGPKGPSAVNVKALQ"
     gene            complement(6950..7249)
                     /locus_tag="PLR13_10425"
     CDS             complement(6950..7249)
                     /locus_tag="PLR13_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19795.1"
                     /translation="MIKKFINNFTTAWKRPEWRWALLFALISDILGFAFTFTPPVYWM
                     IDIVTVLVLFIVIGFKWALLPALVVEMIPGLQLFPAWTLVVLALAGIKNKNESQS"
     gene            complement(7310..7687)
                     /locus_tag="PLR13_10430"
     CDS             complement(7310..7687)
                     /locus_tag="PLR13_10430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC19796.1"
                     /translation="MVERRKEERLKVINEISISVIAIDKIIPLKKGFPFNYSKDISVH
                     GAKIKSEILLPVDTLVKIDFKLKTLGRQITSLGKVKWVKVNIDDKSYDEGVEFIDTPR
                     EAIEKIKFYISQIRKLHETEKKV"
     gene            complement(7811..8467)
                     /gene="nth"
                     /locus_tag="PLR13_10435"
     CDS             complement(7811..8467)
                     /gene="nth"
                     /locus_tag="PLR13_10435"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQC19797.1"
                     /translation="MKDNDINTIIKILKKELTVGTMPIVSHLAENQRDPFVILISTLL
                     SLRTKDEVTAVATDRLFELASTPEEMLKVPLDKIAKTIFPVGFYRVKAKNIHHTCREL
                     IKRFESKVPDDIDTLLTIKGVGRKTANLVVSLAYGKDAICVDTHVHRISNRLGYVKTK
                     TPDETEFALRKKLPRRHWIIYNTIMVAFGRKTCKPVSPLCSQCPVNEYCDQIGVTLSR
                     "
     gene            complement(8649..>8862)
                     /locus_tag="PLR13_10440"
     CDS             complement(8649..>8862)
                     /locus_tag="PLR13_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19798.1"
                     /translation="GPRLMPVPGEIFTELDAIELLTGATASLVAAGGVSGAEGSIWLT
                     ISGTTAQEKAAEALMQSVINEPAFNF"
BASE COUNT         2327 a         1992 c         1775 g         2568 t
ORIGIN      
        1 ccagtggacg gatttaatct tggattcata atacaaacga tgcggaagaa tatccgccat
       61 ttcctctttg acgcttgttt catccgtttt cataaatgat aaggctgatc cgatgatgct
      121 gtattgtgtg tcgacttttc aaggccggac taggactata ggaagtctgc tttttcatgt
      181 caagggaaac ccggtcggcc gataccgcga tgattgtacc ctgagaattt aaggaatgca
      241 tcataaaatt tcgttgcaat gacatcatca ctgcggtaat aaaatgccat gaaaaaagtc
      301 tgtctcttat tatttctctt tttcatccat atcacgctga cggcctgtga cacaaagcag
      361 gaatccgtaa acagccgacc gccggaagaa aaaccgcccg cttacgggga tattctggtg
      421 cggggcgata tcggagacgc cagcaacctg attcccatcc tggcttccga ttcggcttcc
      481 cacaatatcg caggaatgat ttacaacggt ctggtcaaat atgacaaaaa tatgaacatc
      541 gtcggcgatc tggccgaatc gtgggacatt tcatcgaatg gtctggtcat aacattccac
      601 cttcgcaaag gcattaaatg gcatgatggc aagcctttta cctccgccga tgttctttac
      661 acgtatcagg ccaccatcga tccgaaaacc ccgaccgctt acgccggtga ttttttaaaa
      721 gtcaagaaag tcgaggctcc cgatgattac acgttccggg taacgtacga caaaccgttt
      781 gcgccggctc tgatcagctg gagcagcgcc attctgccgc gacatttgct tgcgggaaaa
      841 gacattacac aaagctcttt atcacggcat ccgatcggca ccggtccgta caaattcaag
      901 gaatgggtcg ccggtcagaa aattgtcctt gtttccaatc ctgattattt cgaggggcaa
      961 ccctatatcg acggacggat gacgcgcatt atccccgaca cggccacgat gtttctggag
     1021 ttgcgcgcac aaaatatcgg catgatgggc ctcacgcctc tgcaatacac gcggcaaacc
     1081 gagaataatt tatttaaaaa taattttaac aagtaccggt atttgagctt tgcctatacc
     1141 ttcatgggct acaacctgaa gaatccgctt tttgccgaca aaagagtgcg acaggcgatt
     1201 tcctacgcga tcaacaagga tgaaatcatc agcggcgtat tgctgaagct ggggaaaccc
     1261 gccagcggcc cgtataaacc gggtacgtgg gccttcaacg acaaggtgaa ggtgtacaat
     1321 tacaatccgc agaaagccct tgcactgctc aacgaagcgg gatggaaaaa aaccggcagc
     1381 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaggaaa gaaaaaaata
     1501 ctatgaccgc tttcaggaaa ttctggccga agatcagccc tatacattcc tgtatgttcc
     1561 tgaagcgctg gtaattatca acaaacgttt ccgtggaatc gagccggcgc cgatcggact
     1621 ggaacacaat ttcatcaaat ggtacgttcc gaaagacgaa caaaaatata ccctgacccg
     1681 atagccgcaa caaatcaata cacggttacg cccgcggcat catttccgaa atgaaaattt
     1741 aataaacagg aatgtttttt ttgaacatgt cgatgatgat attttccatg gaataaagac
     1801 cgatgggtgc atcgatcagc catttggaaa caaaaatggc gccctgtccg aaagcccttt
     1861 tgctgatgct ttcatggatg agcctgatgg tctgattggg caggccaaaa ataatctcgt
     1921 gtttgcccac gataccccct acccgaatgg agttgacatg tttatctttc tccagtccca
     1981 ggatattcgc tattttgacg gcggttcccg attgttcctt cttggaccgg aaatgttctt
     2041 ccacaatttc aatatctgca tggggagcaa ctttctggag gatttgagat gccaccagca
     2101 gaaaattgat gcccagcgta atattgggag aataaagaac cgctgttttg gcggaaagtt
     2161 gttttaattc ttccatatcc tttccgccgt atttggagat ggcagaaata attttaattc
     2221 cgacatcggc agccggcgca taactgttgt gcgctctggc gctggaaaaa tccacgatga
     2281 tatccaccgg acgatgagaa aagaattccg gcgatacttc atccaccgaa aagatcgggg
     2341 catcgtctgc ttcatatccc aaaaggcggg tggcatattt atgattgtcc ttatgggttc
     2401 gtctgaccac ccattcgaga tcaaacaatc catccctata aaactcatcc gccaccatcc
     2461 ggccggtatt gccgaatcca aaaagcccga tcttgattct tctgggctgt tgatcattgt
     2521 tgttttgcat ggctaaatcc tttaaattcc gcagctgttt gatcctgcac ggttttacca
     2581 accgataaag ctttcataat aaaaaattaa atgccctgcc atcaggcaga tggcgtccga
     2641 ttatcttaac gcttcatttg tatgttataa gttttggaac cgcttttatg tgaaaaaatt
     2701 tcttcagacc gcatgatatt gcttttttta gttaaataac atgcagatca taaacatgca
     2761 aggcttgcgt tgccctcgga cacagcgttt ttaaaaaata aaaaagcgcg gatcactgac
     2821 cgatccgcgc cgttttttaa taatggtgcc gaagactgga tttgaaccag cacgggtgat
     2881 acccaccacc ccctcaagat ggcgtgtcta ccaattccac cacttcggca tcaaaatgcc
     2941 tcaaaaaaac tgcttatcaa agaatatcgt ttaatatttg cccgatttct tctgctcccg
     3001 atgatccgtt ggggaaaaac cgaatctact tactggccgg tgcaggcggc acggccggca
     3061 tctttgcagc atcaggcccc ggcacaggag atggaagtgc ggttgcgtcc tggccgggcg
     3121 caggcatggt ccgatcaaca gccggcggtc tttgatccat taaagacgac gtgcttctgt
     3181 ggttggccat ataggtaagg atgagagatg tcagcataaa aacgactgcc acccctgcgg
     3241 tcaactttcc caaaaacgtc cccgcgccgg aagaaccgaa aaccgtctgg ctggaaccgc
     3301 cgaaggccgc ccccatattg gcgcctttac ctgcctgcaa aagaacaatc agaatcaaaa
     3361 ccagacaaat cacaatatga agagcaatca ataacgtata catataaatc tcttgtaaaa
     3421 attttatttt aaagcggctg gacaatacca aaacggtata cgctagtcaa cttttttctt
     3481 ttcccagtta ttttaaaaac ttaagtctat tatctcaaag tctgacatat ttcccctcgt
     3541 caatcgctat tcttccgatt ctctctaagt attcaatatg tttgccgacc agcgcccgtt
     3601 cattcgattc aaagaattcc ctgggttccc tcggcctgcc gtagatcagc catgccccgg
     3661 cgatttcgtc cagcgatttg ggttctttca gaaaattcag aattttttct tcacgctgat
     3721 aaatgacatt ttcataatca tcccaaagct ttcccggatt ttcttcaaaa agtccggtgt
     3781 tgtgggaagc aatccatctg cgcgcggaaa tggatttaag cctgcgaata gattgaatag
     3841 tttgttcgat gctggaataa agatccccgt accagggacc gaatgttgtc aaatcataat
     3901 cgccgagaaa aagaatttct tcatcaggaa tgagaaatga gagatgccct ggcgtgtggc
     3961 cgggcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga acctttccca
     4081 attgaacgtc catcgttaat tcatcgtccc ctaagattat ttattttgat ttatacttga
     4141 attgaaaaag aaagtccaat gaggtcgttg ctaataccta cccgtccaca ttattggatc
     4201 gttgtctgat ttgaggattg ctaattgaaa gaatttattc ggggattttg ctccaatggc
     4261 actactatgg taaaatattc ttaacacaca ggacagcact ttctatactg ccgacaacta
     4321 aaagggagtt cttagcaata attccttcat cgtcacaacc gacattggtt ttgattaacg
     4381 cttctgatgg attccctaga atatattatt atgcatgttc tacttcagca atagctaacc
     4441 atttttttgc tttagaaaca tctctaaata cttgaacgtg ccctccccga ttacaggcta
     4501 ctgtttctcc aaattttgca tcttcctccg acattgtctt ttccgaatat acaaccgcat
     4561 acctgaaccc caaaggaaac attttaaggc ttgattcagc aaattgatat atctccataa
     4621 ctgaagcatg cacaatcgcg ttgcgaatat ctattaatat gcgttttgcg ttgcaggttt
     4681 ttattgagtc aacaatttct ttacgcatca ataaataagc tgcttcgttc aattcaccaa
     4741 cagctgcagc gataagagtt tctccatccg aaaagacttt tacaaaatgt tccacaacga
     4801 cctcaaacga gtcagtacat gcagttaatt aagagatttg acggggtcga gaaaggaaac
     4861 agaaacaaat atattaccgc cttttcatag tctacgctct ataatcctat tgtcaaggca
     4921 atacttgatg ctgcagattg ctttttcgat ataaaataaa gctgacagaa accatactgc
     4981 cgtgaattag gcttgggcca cagctgtttt tatagattag tcttctttgt tttaaccggt
     5041 tctttgtaca ctttccattg gtgattcggg tcactcacaa aatctcgatt caccgcttta
     5101 ccatactgat ctgttttaaa ttttctgaca atccatcctc cacggacttt ttgtcgatca
     5161 cttctcacat cgttaccgac agttgttaag atttcccaca ctttaactca ccatccctta
     5221 tttcttgata tataagttct cttgtttttt tgagtaattc ctttgaggcc attaaaacct
     5281 ctatgatgtc acggtttgag tcgcaccaat ttcttctacg atagtcgttg ttgggaaatg
     5341 ctgaaccgtt ttcatccttc atgttaaaat tcctctttta tggttttcgt cgttggccta
     5401 tttcttcttg cctgatgcct tcttctcttt ttcccggacc ttcaggctct gctcccgcct
     5461 tcttcttttg cgggccagtt cctttcttcg ttcgccactt ttctgactca tgatgcctcc
     5521 ttcatattaa atcacgggta ttaagccctt tgcttcgcgg gattgttcaa cttaccaatt
     5581 ctgccgcgct gcactttcgg aatcggagtt ttaacaataa attcattgaa attattgttc
     5641 catttctggc atgaactcta ttgaatggca ggtcgattat tccgcgccct cgacgatgtc
     5701 tgccatccgg cttgattcga ctgtatcaaa atctttgttt gttttgctcg actttatatt
     5761 agccttaatc tgcttggcta tccggcgctt taaatcccta tccatttgtt ttctctgttg
     5821 tgcttttaaa ttggccgtct taccaaagtt gtgcataggc tttcccattt ttaataattg
     5881 tttacctttc tattcaggtg ctgctgaata ggttgaaaat aaaaaggcac tccgggattc
     5941 tgagctcacg gagcgctttt tctattaaca tgattttatt gcagcgcttt cacgttaact
     6001 gccgacgggc ctttgggtcc attctcaata tcaaaactga cgctcgcgcc ttcctgcagt
     6061 gtcttgaaac cgctggcctg aatgtcgttg aaatggacaa aaacatcacc gccatcattg
     6121 tttgcgatga atccgaagcc tttcttttca ttaaaccatt taactttacc ttctgacata
     6181 gctctaatcc tcctttcata aattttcaaa agtcgaatca gcacaatgac agaacccaca
     6241 gttattatac cccacgcaaa ttgcggggta taataatctc cgctttatgg gattgttcaa
     6301 ctgaccaatt ccgctgcgct ttgctttcgg aattggagtt tcacgaataa aaaaaccacc
     6361 aagattccaa agtacctggt ggctagccta tgtgcttcaa aatgtctaaa tccaactctt
     6421 atcttcgcat tttatttcta aatattcgaa gttgacgaaa taatacagta atggcacgga
     6481 atagtcaatc tttatttgca tggataacgt tttgcacggg tagtatggag aaaatatatt
     6541 gcgtttcctc ttataaggca caatgttaag atgttaaggt ttttgaaaga tatatttatt
     6601 attttacggc attctatcaa gattctcctt ttttcaattg gcttttcgga gctttgacag
     6661 agggattgtt gaatggtttg gatgcttatc tggcttccgg ccttcggaat gacggcgggg
     6721 agaaattgca attgaattat attgaaattg aattgacaag agcttgtttt ctttggaagg
     6781 tagtaaaaga aatgcggcct agtatgtcaa gaaatgcttg gccagacata atgaggggtt
     6841 tgcttctata tttgtcgggt acaaacagtg tttattacaa aacgaaagaa agcatatccg
     6901 taggaaatct aaaatactct ctttcaggaa gctatacccg ataaaaagtt tacgattgcg
     6961 attcgttttt attttttatg cctgccaatg ccagcacaac cagggtccac gccggaaata
     7021 actgcaaacc gggaatcatc tcaacaacga gtgccggcaa aagcgcccat ttaaaaccaa
     7081 tcactataaa taatacgagc actgtaacaa tatcaatcat ccagtacacc ggtggtgtga
     7141 atgtgaatgc aaatcccaga atgtccgaaa tgagtgcaaa caacagggcc catcgccatt
     7201 cgggccgttt ccatgcggtt gtaaagttat tgatgaattt tttaatcata ttttactgac
     7261 aatcatttcc ctttgtcaga cagtctctgt tcaagaaaga tcgggcttct tagacttttt
     7321 tctcggtctc gtgtaatttt ctgatctgtg agatataaaa cttgattttt tcgatcgctt
     7381 cacggggtgt atcaataaat tccacgccct catcataaga tttatcatcg atatttactt
     7441 tgacccattt tactttccct aacgaggtga tctgtcttcc cagcgtcttt aatttgaaat
     7501 ctattttgac aagcgtatca accggtaaaa gaatttcgga cttgattttt gctccgtgaa
     7561 cagaaatatc cttgctatag ttgaatggaa atcctttttt aagaggaata attttatcga
     7621 tggcgatgac agagatagag atttcgttga ttacctttag acgctcctct tttctccttt
     7681 ctaccatggc gtttcactcc tctgtctccg agtttaacct caaattttac gtgtaaattt
     7741 tactacaaga aattcagttt gtatataaag gaactttgat cacctaaaac cgggctttgt
     7801 tcaggtaata ctaacggctc aaggtaacgc cgatttgatc acaatattca tttaccgggc
     7861 actggctgca aagcggagaa actggtttgc aggttttacg gccgaaggcc accatgatgg
     7921 tgttatagat aatccagtgg cggcgcggca gttttttgcg cagggcaaat tcggtttcat
     7981 ccggagtttt tgttttgaca taaccgagcc ggttggaaat tctgtgcaca tgcgtgtcca
     8041 cacagatggc gtccttaccg taggccagcg acacaaccag attggcggtt ttgcgtccca
     8101 cacctttaat ggttaacaaa gtatcaatgt cgtccggcac tttggactcg aagcgtttga
     8161 ttaactcccg gcaggtatgg tgaatgtttt ttgccttgac gcgataaaag ccaaccggaa
     8221 aaatggtttt agcgatttta tccagaggaa ctttgagcat ctcttccggt gtggaagcca
     8281 gttcaaagag tctgtcggtg gcgactgcgg tgacttcgtc tttggtccgc aggctgagca
     8341 atgttgaaat taaaatgaca aagggatcgc gctggttttc agccagatgc gaaacaatcg
     8401 gcatggtccc cacggtcagt tccttcttta atatttttat gatggtgttg atgtcgttat
     8461 ctttcatgta ttaattttca aatattcctt taataaattc agcaattccc ataaagatgc
     8521 agccaaggta gatgtcattc cgggacatga caaagcttca caacactgct tcgaaggtgg
     8581 aacccgggta atgtttatta tttcctggat tccgggtcaa gcccggaatg accaatgaaa
     8641 ggatattttt aaaagttaaa tgccggttcg ttgatgaccg attgcataag agcttccgct
     8701 gctttttcct gagcggtcgt tccgctgata gtcagccaga tgctgccttc cgcgccgctg
     8761 acgccgcctg cggcaaccag cgaagccgtt gccccggtca gaagttctat ggcatccagt
     8821 tccgtgaaga tttctcccgg cacgggcatc agtctcggac cc
//
LOCUS       DAONQZ010000087         8707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8793_length_8707_cov_11.909501, whole genome shotgun sequence.
ACCESSION   DAONQZ010000087 DAONQZ010000000
VERSION     DAONQZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8793_length_8707_cov_11.909501"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1412
                     /locus_tag="PLR13_10445"
     CDS             93..1412
                     /locus_tag="PLR13_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQC19799.1"
                     /translation="MQLKRELNLADATLLVVGNVVGAGIFTTSGFLARELPHPLFFIG
                     IWVLGGLVTLLGALTYAELAGMFPKAGGDYLFLKAGYGSWAGFLLGWINFWIIIPGSI
                     AALSLAAVSYLKPLLILNNPLSEKGLALGIIFFFSLINYRGIRWGGTTQDFFTLGSLM
                     ILMIFIAAGLMFGNGSWGHFSAVSTQAPPVSKIFGSAMIAIVFTYSGWFASAYVGSEI
                     KNPGRNLPLSLLLGTVIVSILYTLINVTYLYALPLTGLAGIVNVGQETAAALFNPAIS
                     LLISVAIILAICSSINATIMAGSRIFYAMSEDQILWSFLKRLHPRFHTPHLAILSQTV
                     LAALLVCLGTFDHLLSYVVFTIIIISVATGTAHLVLRWNRPALARPYRTWGYPIVPVL
                     FIIVYLWIGGQIFIEKPATSLIGLCLAVSGLPFYFFWTRSNKSIEGG"
     gene            1415..2797
                     /locus_tag="PLR13_10450"
     CDS             1415..2797
                     /locus_tag="PLR13_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQC19800.1"
                     /translation="MTKKKIFHYGGFDMRKMVCLFFILMIALFLGGCSSGSDDPTYSV
                     KGVVVDDNGSGLAGVTVELSDDASGTAGAVAVGGAVIETATTDANGNYIIAGIEAGDY
                     TITPKREGYIFVPATIPVTVSNADVDLSTDDNKKIAARKDNSDDAVQTASGFLHYTLP
                     DQYQYEQDGGLIANEAAALFSGYTLTNGIDYGKGTEVSGYALDQFVDKAAVNAATPDP
                     DGVLETHDARKLYSVVLRSKQDGFSNRTKFIGQGYYNADLTWDQFVLGYLLDLNYSGK
                     VHYPRSVAEPPTQVKMYNIKYVYDIYMFRKIDVKRPDADGTIATFEVAATADNHVDDA
                     TYATSTGLTTTKFEVSKKSFGAYTNVNVIPLTQFLTDYVTGTPASYTYKIIALDDTKS
                     QTGWTYAAMQQAYYLPDYDFIIQIDASGNVVAGTKINFPVRIELISAAAVEYDYAAKN
                     PPAYAKAYDE"
     gene            2919..>4150
                     /locus_tag="PLR13_10455"
     CDS             2919..>4150
                     /locus_tag="PLR13_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQC19801.1"
                     /translation="MLGWITFLVLATATTLMAQETYKAENADETKPQIQPLKEQDRRE
                     NKTKTETDYQLEGIVVKERRIKNIEKSITVKGGTSTEVTAQDIEERSDKVLKDVLYQV
                     PGIQVSTQRKGTTQFYMRGYDMSKVAMLVDDIPLIESYTGSMDIDNIGLLDISEIVVS
                     RGTTSALYGTRGGVGSINLVRKKPVKMYTDVSAEYGEHDNFVASVSHGAPVGNFYYQL
                     SALYDKSGGYEISEKLDRKEREKWLKKLSRYDLYGFTRNDIYSHPGSSAAVYYLDETG
                     TWKHTQHEKYKLNGKFGYHMTPDLDMGLSLFYNQTEKENSSYFTDMRSMYTYNSYTQD
                     KDWRLPDTTYVLRNMSSRWPEYNDYAVSPYVIYEKGRFGIKANAYFYENSNKFMAYDD
                     PLERVLAYNRDVDTMTWS"
     assembly_gap    4151..4250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4251..5204
                     /locus_tag="PLR13_10460"
     CDS             <4251..5204
                     /locus_tag="PLR13_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQC19802.1"
                     /translation="DNMDLTLGVSYDAQDLKHFRKKLGINGSTDMIDQYEAEDDSMLW
                     GTRDSLNPVAGILYEPVKDFLKLKASASRKTSFPSLQAYSKTVSPYQTSSDLGSRDVN
                     IKPEKMLNGNLGFEFSFFNKKLTWGADYFYSKYDDKITKIYITKIDDYIFRNIDSAVI
                     HGAETTMTLNFINVFDMADISFSSTYTYIHSRNQADIDDSFVNKGDKFERLPEHKFTF
                     DFRTYFKTNTSLIIFGNFEFNQIQYVMSSIPKATDDFSTSYYNALELHNPLKIDIKLS
                     QKIFKNYEVYLMCKNILDDYNADPFDPGPGRMWYAGVKASF"
     gene            5237..5671
                     /gene="exbB"
                     /locus_tag="PLR13_10465"
     CDS             5237..5671
                     /gene="exbB"
                     /locus_tag="PLR13_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HQC19803.1"
                     /translation="MEWLRVVIDYGIIGLLIFLSMIAIGIAIERFYFFRHIRLDTFPD
                     KKSLELELTKKIHLIATIGSNAPYIGLLGTVLGIMLTFYMIGKEGFMDTGKIMVGLAL
                     ALKATAIGLLVAIPSVSLYNFLLRKVKVLLMTWEIEHGRKGI"
     gene            5652..6032
                     /locus_tag="PLR13_10470"
     CDS             5652..6032
                     /locus_tag="PLR13_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQC19804.1"
                     /translation="MEEKEFDYINVVPLVDVMLVLLTIVLTTSSFIASGMIPMDLPKA
                     HSRAGETLKTQTIEIDRTGGIYLNSQPVSLEGLQTDLDRMNRNIPVLIRADRSTSLQN
                     FVSVLDIIKGLDFTRVSLQTEETK"
     gene            6029..6778
                     /locus_tag="PLR13_10475"
     CDS             6029..6778
                     /locus_tag="PLR13_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQC19805.1"
                     /translation="MNYQTRAFQISFLLHSLIVVLIVIGSTYIAQHKKEIVLDFDLQK
                     PVPDVERVETPVPAPSVEKKFASPAVRQNVQEKEPSPSRPPEESPRPSSIPETPPVVK
                     LPETRNQETSPVGQGTTDTAKAVKEGTPGTVTMAREGTGMNPGVGNANGGKEAVVTKY
                     LNENFAYIRDRILKNVSYPDTARRKGWEGKVVLSFVIMANGSVKAFKIIQSSGFTVLD
                     KSAIETVRDTAPFPRPPGEAQLVIPITYQLE"
     gene            6834..7754
                     /locus_tag="PLR13_10480"
     CDS             6834..7754
                     /locus_tag="PLR13_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQC19806.1"
                     /translation="MSKQFHGRVIFWGTLISLLLYAGGVLAASGKFFIVGMGTVPDLM
                     TLRAVQCVKQADIVILEGEEDKAAWKDLIEGKEIWFFGHRSQVFYGVDPQTLKSEAAK
                     AKAIQMAKYRQDMMDKIKDAVAKGKNVASLQWGDPMMYGITLFLEMLPKNIPSEIIPG
                     VGAFQASSAAVKMSPPYGWDTNGVILTMADWPGRTDTNEKLMATQSSMVFFTMHLDYP
                     KLFEQLKRHYPKDTPVAVVNHAGDRERQKVILSTVGGFLDDVKYQELPEEAHMLMVGK
                     FLKVGQSRKEGLTHGKDYIEGRHFNGEDKK"
     gene            7928..>8707
                     /locus_tag="PLR13_10485"
     CDS             7928..>8707
                     /locus_tag="PLR13_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19807.1"
                     /translation="MKKILCNKKYIVSFYFILFCFCHLMQAPQSCAGEMGLSFIAEYF
                     NWKEFVDDHRLLKESGPRFGIGWDYHAEFFDKHLILKPRIEIIAGQVDYNGATQSDVH
                     VSSDTDYYEGKAEVDIGWQVGLSENFSVVPFGGIGLRGWYRKIHGATASDGLTRAAGY
                     PEEWYSLYLKSGFRGVFSLNENLHLFVEAGGKFPVYNENKALFDESDLGPNVTFKTGN
                     DLSWFIEAGLQYKSFKSSIYYDGMRFSKSDIEYSHGMRYYQP"
BASE COUNT         2349 a         1879 c         2127 g         2252 t
ORIGIN      
        1 tctggaagcg gtccgcgacg aaaatgaaga aaaggagtcc tggctgggaa tgatttatgt
       61 cggcttctgc ctgaaataaa ggaaacggag acatgcagct taaacgggaa ttgaatcttg
      121 cggacgccac cttgctggtt gtcggcaatg tggtcggcgc gggaatattt acgaccagcg
      181 gctttttggc cagggaactt ccccatccat tgttctttat cgggatatgg gttttggggg
      241 gactggttac ccttttgggc gcgctgacct atgccgagct ggccgggatg tttccgaagg
      301 caggtggcga ctacctgttt ttaaaagccg gctatggatc gtgggccgga tttcttctgg
      361 ggtggatcaa tttctggatc attattccgg gctcgattgc cgctctttct ctggcggcag
      421 tcagttattt gaaacccctg ctgattttga ataatccgtt atcggaaaag gggctggcac
      481 tgggaataat attttttttc tccttgatta attaccgggg cattcgctgg ggagggacaa
      541 cgcaggattt ttttactctg ggttctctga tgattctgat gatctttatt gccgccggtc
      601 ttatgtttgg aaacggcagc tggggacact tctctgccgt ttccacgcaa gcacctcctg
      661 tttcaaaaat tttcggttcc gccatgattg ccatcgtttt cacgtacagc ggatggttcg
      721 cctcggctta tgtgggcagt gaaattaaaa atcccgggcg caatcttccc ttatccttgc
      781 tgttgggcac ggtcattgtc agcatccttt atacgctgat caatgtcacc tatctctatg
      841 ctctgcctct cactggactt gcaggaatcg ttaatgtggg gcaggagacc gcagccgctc
      901 tgtttaaccc cgccatctcc ttgctgattt ccgtggctat tattctggct atttgttcca
      961 gtatcaatgc caccatcatg gccggatcgc ggatctttta tgccatgtcc gaagatcaaa
     1021 tcttatggtc ttttttaaag cgtcttcatc ctcgttttca cacaccgcat ctggccattt
     1081 taagtcaaac cgttttggcc gctcttttgg tttgtctcgg aacgttcgat caccttttga
     1141 gctatgttgt ttttaccata attattattt ccgtggccac cggtaccgct cacctggttc
     1201 tgcgatggaa caggccggca ctcgcccgtc cataccggac ctggggttac ccgatcgttc
     1261 ccgttctatt cattattgtt tatctctgga tcggcggaca gatttttata gaaaagcccg
     1321 caacctcatt gatcggactt tgcctggccg tttccggatt gccgttttat ttcttttgga
     1381 cgagatccaa taaatcaata gaaggaggat gaacgtgacg aaaaagaaaa tatttcatta
     1441 tggaggcttt gatatgagaa agatggtctg tttatttttt attttaatga ttgcactgtt
     1501 tcttggcggg tgttcgagtg gaagcgatga ccccacctat tccgtgaagg gcgttgtcgt
     1561 cgatgacaac ggttccgggc ttgccggcgt taccgttgaa ttgagtgatg atgcgtcagg
     1621 cacggcaggc gcggtggcgg tcggtggcgc cgtaatcgaa acggcgacaa ccgatgccaa
     1681 cggtaattac ataatcgcag gcattgaggc gggcgattac acgatcacgc cgaaaagaga
     1741 aggatatata ttcgttccgg caaccatccc tgtcacggtc agtaatgccg atgtcgatct
     1801 ttctacggac gacaacaaaa aaattgctgc cagaaaagac aatagtgatg atgcagtcca
     1861 gacggcctcg ggttttttac actataccct accggaccaa tatcaatatg aacaggacgg
     1921 gggtcttatt gccaatgagg cagccgcgct attttccggt tatacgttaa caaatggaat
     1981 tgattacggc aagggaacgg aggtttcagg gtatgcgctg gatcagtttg tggataaggc
     2041 tgccgtcaat gcggcgacgc cggatcctga cggcgtgtta gagacacatg atgcccggaa
     2101 actatattcc gtagtcctcc gctccaagca ggacggattc agcaaccgga caaagtttat
     2161 cggccagggg tactataatg ccgatttaac gtgggatcag tttgtcctgg ggtatctgct
     2221 ggatttaaat tacagcggga aagtgcatta tcccagatcg gtggcagaac ctccgaccca
     2281 ggttaaaatg tataatatta agtatgtcta tgacatctat atgttccgga agattgatgt
     2341 gaaaagacct gatgccgacg ggactatcgc gacatttgaa gtggcagcca cagcagacaa
     2401 tcatgtggat gatgcaacct acgcgacaag cacgggatta acgacgacaa aattcgaagt
     2461 ttcgaagaaa agtttcggag cttacacgaa cgtaaacgtt attccgctga cacagtttct
     2521 cacggattac gtgaccggaa cgccggcttc ttatacctat aaaattattg ccctggatga
     2581 cacgaaatct caaacaggat ggacgtatgc cgccatgcag caggcgtatt atttgccgga
     2641 ttacgatttc atcattcaga tcgatgcctc cggcaatgtg gttgcgggaa ccaagatcaa
     2701 tttccctgtc cggattgaac tgatcagcgc cgctgccgtt gagtatgatt atgccgcaaa
     2761 gaatccgcca gcgtatgcca aggcatatga tgaatagcga atgttgactg agcttgcttg
     2821 acgagtatca agccggataa accatcgtga atgccgattg acctaccggc attcacgatg
     2881 tctgtaaaaa aacggagaac agggttttaa tcatgagaat gttgggatgg ataacgtttt
     2941 tagtgcttgc aactgctacg acattgatgg cgcaggaaac atataaagct gaaaatgcag
     3001 acgagacaaa accgcagata cagcctttga aagaacagga ccgccgggaa aataaaacga
     3061 aaaccgaaac ggactatcag ctggaaggaa ttgttgttaa agaacgccgc atcaaaaaca
     3121 tagaaaaaag tataacagta aaaggcggca cctcaaccga ggtcaccgca caggatattg
     3181 aagagagaag tgacaaggtg ctcaaagatg ttctgtatca ggtgcccggg attcaggtat
     3241 cgacacaacg aaagggaaca acccagtttt atatgcgcgg atatgatatg tcgaaagtag
     3301 ccatgctcgt ggatgacatt cctttaatcg aatcctacac cggcagtatg gatattgaca
     3361 acatcggtct gcttgacata tcggaaatcg ttgtcagccg cggcacaacg tcagcactat
     3421 acggcacacg gggcggagtc ggttccatca atcttgtcag aaagaaaccc gtcaaaatgt
     3481 acacggatgt ttccgccgaa tacggcgaac atgacaactt tgtggccagt gtttcacatg
     3541 gcgcacccgt gggcaatttt tattatcagc tgtctgctct ttatgacaaa tcaggcggat
     3601 acgagatctc ggagaagctt gaccgcaaag aacgcgaaaa atggctgaaa aagctttcgc
     3661 gatatgatct ttacggattt acccgcaacg acatctactc gcatccgggc agttccgctg
     3721 ccgtttacta tctcgacgaa acgggcacat ggaagcatac gcaacacgaa aagtacaagc
     3781 tgaatggtaa attcggatac cacatgaccc ccgatctgga tatggggctg tctttatttt
     3841 ataaccagac ggaaaaggaa aacagttctt attttaccga catgcggagc atgtatacct
     3901 ataacagtta tacacaggat aaggactgga ggctgccgga tacaacatat gttttgcgca
     3961 acatgtccag ccgctggccc gaatataacg attatgcggt atcgccgtat gtgatttacg
     4021 aaaaaggccg attcgggata aaggccaacg cctatttcta tgaaaattcc aacaaattta
     4081 tggcgtatga cgatccgctg gaaagggttc tggcctataa ccgggatgtc gacaccatga
     4141 cctggtccat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gataatatgg
     4261 atttgacgct gggtgtctct tatgacgcgc aggatctgaa gcatttccgg aaaaaactgg
     4321 gaatcaacgg aagcacggac atgatagatc aatacgaggc cgaggatgat tccatgctgt
     4381 gggggacgcg tgactctttg aatccggttg ccggtattct gtatgaaccg gttaaggatt
     4441 ttttaaagtt gaaggcttcc gcatcccgga aaacctcttt tccctcgctt caggcctatt
     4501 ccaaaacggt gtctccctat cagacgtctt cagacctggg gagcagggac gtcaatatta
     4561 aacccgaaaa aatgctcaac ggcaatctgg gctttgaatt ttccttcttt aacaaaaaac
     4621 tgacatgggg cgcggattac ttttacagca aatatgacga taaaattaca aaaatatata
     4681 ttacgaagat tgacgattac atcttcagaa atatcgattc agcggtcata catggcgcgg
     4741 aaacgacgat gactctcaat ttcataaacg tcttcgatat ggcggatatc agtttctcat
     4801 ccacctatac atatatccat tcgagaaatc aggcggatat cgacgattcc tttgtcaata
     4861 aaggcgacaa attcgagaga ctccccgaac ataaattcac ttttgacttc aggacgtact
     4921 ttaaaacaaa caccagctta ataatattcg gcaactttga atttaatcag attcagtatg
     4981 tcatgagctc catccccaaa gcgacagatg atttctcaac atcctattac aacgccttgg
     5041 agctgcacaa tcctttaaaa atcgatatca aattatcgca gaaaattttt aagaattacg
     5101 aggtctatct gatgtgcaaa aacatcctgg atgattataa cgcggatcct ttcgatccgg
     5161 ggcccggcag gatgtggtat gcaggcgtca aggcaagctt ttaacgggga attttacaca
     5221 acggtaagga gaaaaaatgg aatggcttag agttgtcatt gattacggga ttatcggact
     5281 attaattttt ttaagcatga ttgccatcgg gattgccatc gaacggtttt atttttttag
     5341 acatataaga ctggatacct ttccggataa aaaaagtctt gaactggaac tgacgaagaa
     5401 aatccatctt atcgccacca tcggcagcaa tgctccttat attggcttgc tcggcacagt
     5461 gctgggcatt atgcttacgt tttacatgat cggcaaagaa ggttttatgg acacggggaa
     5521 gattatggtg gggctggcgc tggccttgaa ggccacagcc atcggccttc tggtggccat
     5581 cccttctgtt tctttgtaca attttctact gagaaaagtc aaggttctgc tcatgacctg
     5641 ggagattgaa catggaagaa aaggaatttg attatatcaa tgtcgttcct ctggtggatg
     5701 tcatgctggt gctcctgacg attgtcctga ccacgtcttc gtttatcgcc agtggcatga
     5761 ttcccatgga cttgcctaaa gctcacagcc gtgccggaga aaccctgaaa actcagacga
     5821 ttgaaatcga ccgaaccggg ggaatttatt tgaattcaca gccggtatct ctggaaggat
     5881 tgcaaaccga tctggatcgt atgaatagaa acatccctgt tttaatcaga gcggatcgca
     5941 gcaccagcct gcaaaatttt gtcagtgttc ttgatatcat caaaggattg gactttaccc
     6001 gcgtcagcct ccagaccgag gaaacaaaat gaattaccag acaagggcat tccagatatc
     6061 attcctgctg cacagtctga ttgttgttct gattgttatt ggcagcactt atatagcaca
     6121 gcacaagaag gagattgtcc tcgattttga tttgcaaaaa cctgtgcccg atgtcgaaag
     6181 agttgagacg ccggtgccag cgccttctgt agaaaaaaag tttgcaagtc ccgccgtccg
     6241 tcaaaacgtg caggaaaagg aaccttcccc ttcccggccg ccggaagagt caccccggcc
     6301 gtcatccatt cctgaaacgc cgccggtcgt gaaacttccc gaaacccgca accaggagac
     6361 cagcccggtc gggcagggaa caactgatac agcgaaagcg gtcaaagaag gaactcccgg
     6421 aacggtgaca atggcaaggg aaggaacggg aatgaacccg ggcgtcggca atgcgaatgg
     6481 cggcaaagaa gcggtcgtga caaagtattt aaacgaaaat tttgcttata tccgggacag
     6541 gattctgaaa aatgtcagtt accccgatac cgcgagaaga aagggatggg aaggaaaggt
     6601 tgtgctttcc tttgtcatta tggccaatgg atctgtgaag gcgttcaaga ttattcaaag
     6661 ttcaggtttc accgtgctgg ataaaagcgc catagagact gtccgtgaca ctgctccgtt
     6721 tcccaggccg cccggtgaag cgcaacttgt gatacccatt acatatcagt tggaatagcc
     6781 gtatatacag gttttaagaa gctaaaatta aatagaataa acgggaggaa aaaatgtcaa
     6841 agcaatttca tggtcgggtt atattttggg gaactcttat ttcccttttg ctttatgcag
     6901 gcggcgtact tgccgcatcg ggaaaatttt tcatcgtcgg catgggcaca gtgcccgatc
     6961 ttatgacgct ccgtgccgtg caatgcgtca agcaggcgga cattgttatc ctggaaggcg
     7021 aggaggacaa ggcggcatgg aaagatttga tcgaaggaaa agaaatctgg ttttttggtc
     7081 atcgcagcca ggtgttttac ggcgttgatc ctcaaacgct gaagagtgaa gcggcaaaag
     7141 caaaagcgat acaaatggca aaataccgtc aggacatgat ggacaaaata aaggatgccg
     7201 tcgcaaaggg gaaaaatgtc gcctccctgc aatggggcga tcctatgatg tacggtatca
     7261 ccttgttcct ggaaatgctg ccgaagaaca tcccgtcgga aatcattccc ggggtcgggg
     7321 cgtttcaggc ttccagcgcg gcggtcaaaa tgagtcctcc ttacggctgg gataccaatg
     7381 gcgtgattct gacgatggcg gactggccag gtcgtaccga caccaatgaa aagttaatgg
     7441 caacgcaaag ctccatggtc ttcttcacca tgcatctgga ttatccgaag ctcttcgagc
     7501 agctcaaacg tcattacccg aaagatacgc cggttgctgt cgttaaccat gcaggcgacc
     7561 gggaacgtca aaaagttatc ttgagcactg tcgggggatt tctggacgat gtgaagtatc
     7621 aggaactgcc tgaggaagct catatgctga tggttgggaa attcctcaag gtcgggcagt
     7681 cccggaaaga agggttgacc cacgggaaag attatataga aggaaggcat tttaacggag
     7741 aagataaaaa ataatatctc ataaataaag ttgtccttga attgattttt tgtgaacgat
     7801 gcagcattgt gtcggtgaga tcaatgccct tgcttgggaa taaaagtcac aagcggtaat
     7861 agaaaccctt tcgtttatga ggatttcaaa tcatgatgct gaatgctaaa ttcaaagatt
     7921 attgctcatg aaaaaaattc tatgcaataa aaaatatatt gtttcatttt attttattct
     7981 tttttgcttt tgccatttaa tgcaggcgcc tcaaagttgc gcaggagaga tgggcctgtc
     8041 ctttattgcg gaatatttta attggaaaga gttcgttgat gatcacagat tattaaagga
     8101 atccgggccc cgcttcggca ttggatggga ttaccacgct gaatttttcg acaaacatct
     8161 tattctgaaa ccccgcattg agataatcgc aggacaggtt gactataatg gtgctaccca
     8221 gtccgacgtt catgtaagct ctgatacaga ttactatgag ggaaaggcag aggtcgatat
     8281 cggctggcaa gttggattat cggaaaattt ttccgtggta ccgtttgggg gtatcggatt
     8341 acgcggatgg tacaggaaaa tccatggagc gaccgcctct gatgggttga ctcgcgcagc
     8401 gggttatcct gaggaatggt attccttata tcttaaatcc ggattcaggg gtgttttttc
     8461 attaaacgaa aatcttcatc tgtttgttga agccggaggc aaatttccag tatacaatga
     8521 gaacaaagct ctctttgatg aatcggatct gggaccgaat gttacattca agaccggtaa
     8581 tgatctttct tggttcattg aagcaggcct tcaatacaaa tcgttcaagt cgagtattta
     8641 ttatgacggt atgcgctttt caaaatcgga tatcgaatac tcgcatggta tgcgttatta
     8701 tcaaccg
//
LOCUS       DAONQZ010000088         8633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8876_length_8633_cov_9.246794, whole genome shotgun sequence.
ACCESSION   DAONQZ010000088 DAONQZ010000000
VERSION     DAONQZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8876_length_8633_cov_9.246794"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..413)
                     /locus_tag="PLR13_10490"
     CDS             complement(132..413)
                     /locus_tag="PLR13_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HQC19808.1"
                     /translation="MRTKTEQPKKYGVLKYKPYLQMRKEYDFSKAKKNPYASMLKKQV
                     TIRLDERTVDYFKNLAGEAGIPCQTLINLYLRDCAASERKLFMHWKAAS"
     gene            complement(410..925)
                     /locus_tag="PLR13_10495"
     CDS             complement(410..925)
                     /locus_tag="PLR13_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19809.1"
                     /translation="MDKISPEQAMEILRRSARSDPEIGKRIEKVAEEMLKDIDSEAIC
                     EDVYLVLDGIDVLELWDRSGSSRYGYSAPEDMAVEMMEEELAPFNREVLRYLEMGMAK
                     EAKLYCMGVLKGIYRYEQESKSEFKDWAVDIPGECFAHTLREWKKKSGDRNDRREMDE
                     FIKRECGKWAK"
     gene            complement(1358..5101)
                     /locus_tag="PLR13_10500"
     CDS             complement(1358..5101)
                     /locus_tag="PLR13_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQC19810.1"
                     /translation="MFKMQRQHLHKALMIYVKIFHVLSKSLLFVFRCRIYIGRRPADV
                     KAPSLIFFPVLPGRLNCGFAGLMTCRLPSQLSEKTADRRLNSLWGKVKNGGLQNVPAG
                     EKIAEHYLYGDDALHDMGMALSDLKCEAMQELLFFHADRANDLSNLAAEMKAFITNEE
                     KLLEERAELISSFDLEVINSRMLWFKDICWILERDVLANLQKIRDLTGAEKNTEVKTA
                     AFRKYRKLNFLLNALDRLEVRGRDSAGIQLAFVLKNEKAMPDVIRQIRENGLSEDYEQ
                     RTKKGDLLNLSIFVSANQGDAQAGTCVIFTYKTYSIIGELGRNVADLRSSISNDKVLK
                     CFANEETTCETTMTHTRWASVGAITEENCHPVNNHTTGFLSSNDPLYPGASAQINVVL
                     NGDIDNYTALRQPLEHQGEIIARSITTDTKIIPLQIEKYLQLGKNLEEAFRLAVNDFE
                     GSHAIAMTSNLEAGKMFLALRGSGQAIYVGVNEDQYMFSSELYGLVEVMPRFLKMNGE
                     DGGQIFILDQKKGGGIRNITARNYNGELLKLTDASLQDSEITTRDIHRNGYPHFFLKE
                     IFESSLSVKRTLRGKYRISADNQSRSRVSFNLGTDVVPAEISAKLGSGSIKNIVIIGQ
                     GTAAVAAQSVSDALTHYLEGATLNIFVRVASELSGFGLKDNLADTLVIPITQSGTTTD
                     TNRAVAMARERGAHVISIVNRRQSDITAKSHGVFYTSDGRDVEMSVASTKAFYAQIIA
                     GQVLALFFAQLLKTRSDDEIAGQLRHLESAPRLMDQICSGRQAVAATVKKAIGKKYWA
                     VTGSGPNKAAADEIRIKLSELCYKTISSDIVENKKHIDLSAEPFILVCAAGNPETVMD
                     DIVKDVAIFKAHKAAVVVFANAGDHRFDQVADAVVGIPAAPSPLPVILNTMAGHLWGY
                     YAASAIDGEAQIFREFRGRLSVELTQRRQENLSVFDLVADVSLRRMINEFYALFNAHR
                     QSGAFGALGGKTMTDLMLLLKYAAGKLPLQEIRQDFKEEKDLFSPYDLLDMTLGTAID
                     ELTRPIDAIRHQAKTVTVGTSRKEKELEGIVFGLLETLQFSIKALTYRDILMIKRTQP
                     AIAEIRGYTLYDIHHLDDQGRPSADSMIAIRTKAGIAANMKSRADNPTALMGVKRTIV
                     SMGHAYVGRGKTDGASIMIIPLRSEEAGIGNLLLVHIRFNEALTLPEKIEVLGPRYNE
                     IRDLIDEYNLPWNDRYLEIIPLEFLFSEPIDIIAGKIKARQGQ"
     gene            complement(5103..7694)
                     /locus_tag="PLR13_10505"
     CDS             complement(5103..7694)
                     /locus_tag="PLR13_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQC19811.1"
                     /translation="MTPYARVSTGINGLDKILNYLQMGDNVVLQVDDVEDYKKFVAPY
                     VQETLAMNQRLVYMRFANHPALLEAGPGIKIYTLNANSGFESFSTEVHKIISDEGRDV
                     FYVFDCLSDLLQAWATDLMIGNFFVITCPYLFELNTVAYFAILRSRHSFKTVARIRET
                     TQVLLDVYNNKGKLCVHPLKAWKRYTPTMFLPHIMEGDKFVPILNSADAASTLSYLTD
                     KNATSGARNLDYWDRIFLKATNILEDPKAFQEKQEMVETLSRMLIGREKRMLSLVKEY
                     FSLEDLLEIKKRLIGTGFIGGKSVGMLLARNILRNDTTFAWNAELEIHDSFYIGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFEVAHELKSKMLHGVFPDEVKEQFQLMLEYFGQSPI
                     IARSSSLLEDAFGNAFAGKYESYFCVNQGTPEERYRYFEETVRKIFASTMNEDALTYR
                     LQRGMAQHDEQMALLVQRVSGSHRATYFFPDLAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPLTKPSAKQEDIKRFSQSEVDILNLQANNFQTLPA
                     ADLLTESIEAHLDMIATRDVAASEYMQSLGKPAKDAWILTFDELLENTAFAQTMSNML
                     RKLEQVYRYPVDIEFTVNFNAQGKTQINLLQCRPFQTKGHYSRVELPEKINKSKILLQ
                     QEANFMGGSIYQAISRIIYIDPKEYAGLSLSEKYEVARLTGKLNKQIGRREVMPTILL
                     GPGRWGTTTPAMGVPVKFSEINNITAIGEIAYQDGSLIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKSDVFFNTAWMKKYPNILTSLLPDDKAFADVVRVFDVREKDLRLLSDIVTQK
                     MVCFFGK"
     gene            complement(7920..8117)
                     /locus_tag="PLR13_10510"
     CDS             complement(7920..8117)
                     /locus_tag="PLR13_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19812.1"
                     /translation="MEDSPRQASENLPSLGKNSDFHVDCFFMPQRPANFPSAGFKFGI
                     GSKMFHGSPVKEREEKGANVI"
     gene            8312..>8633
                     /locus_tag="PLR13_10515"
     CDS             8312..>8633
                     /locus_tag="PLR13_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQC19813.1"
                     /translation="MNSGDTAWVLTASALVLLMTIPGLVFFYGGLVRRKNVLSILMQC
                     FIIVCVISLQWVLFGYSLAFGPDFHGLIGKLDWVGLSGVGAVPNKDYAATIPHSAFMI
                     FQAMF"
BASE COUNT         2059 a         2188 c         2038 g         2348 t
ORIGIN      
        1 gtgatccgca aagcaggaat agtgcggttt cgggaacaaa caggtgtctc ctttagggct
       61 caacgaaaag ctcgcccgga gcaacgcctc cggcgaggcg atcggcccaa gcgaccggtt
      121 gtgcagaaat tttatgatgc tgctttccaa tgcatgaaaa gcttacgctc agaggcggca
      181 caatctctta aataaagatt gattaaggtt tggcaaggaa tacccgcctc accggcaaga
      241 tttttgaaat aatctacggt tctctcgtct aaccttatgg ttacctgctt tttgagcatg
      301 cttgcataag gattcttttt tgctttggag aaatcatatt cttttctcat ctgcaaatac
      361 ggtttgtatt tcaagacccc atattttttc ggttgttctg ttttggttct catttcgccc
      421 acttgccgca ctctcttttg atgaactcgt ccatttctct gcggtcgttc ctgtcgcctg
      481 attttttctt ccactcccgt aatgtgtgcg caaagcattc accgggaata tccacagccc
      541 agtctttgaa ttccgatttt gactcctgtt cgtaccgata tattcccttc agaacgccca
      601 tgcagtagag cttggcctct ttagccatgc ccatttcaag gtatctcagg acttctctgt
      661 taaatggggc cagctcttct tccatcatct cgaccgccat atcttctggc gctgaatagc
      721 catatctgct ggatccggag cgatcccata attccagaac atcgattccg tccaagacca
      781 aatagacatc ttcacagatt gcttcgctat ctatgtcttt gagcatttct tcagccactt
      841 tctcaatccg ctttccgatc tctggatcgc ttcttgccga acgcctcagt atctccatgg
      901 cctgttcagg agatattttg tccatttttc ttttcactct tcaccgaacg acgaaatcac
      961 ccggagcgca gcgtgtcggc tgcatcaatg ggttagctgt ttttacatgc atttacaaaa
     1021 tcatcggcat tcaattgaat tgcccttgct tgctgtgcca atctccgcgc acacagcgac
     1081 atataaagcg tcctctttat gaatgatccc tgtaaatatt ttattattca tatgtcgttt
     1141 ccttctttga tatttttcac atattttatt cttatcatcc aaccagcgga acggccggtc
     1201 cgtgtggatt gcatggttga gtaatcaata aatagaaccg tccccattat ttcttaacag
     1261 aagccctcgc caaatcaaga actaacagaa ctaactcgac caacacaaca tcggcgaaag
     1321 tttcacaaat cagattttgg aaaaaaccgc aatgccccta ctgtccctgc cgggctttta
     1381 tctttccggc gatgatgtca atcggctcgc tgaaaaggaa ttccagggga attatctcca
     1441 gatatcggtc gttccatggg agattgtatt catcaataag atcccgaatt tcattatacc
     1501 gtggtcccag cacctctatt ttctccggca acgtcaatgc ttcgttaaag cggatatgca
     1561 ccagcagcag gttcccgatg cctgcctctt ccgacctcag gggaataatc ataatggatg
     1621 ccccgtccgt tttgccccgt cccacatagg catggcccat gctgacgatt gttcgtttca
     1681 cgcccatcag ggctgtcggg ttatccgctc gcgacttcat gttggcggca atgccagctt
     1741 tggtgcggat ggcaatcatc gaatcggcag acggacggcc ctgatcgtcg agatgatgaa
     1801 tatcgtaaag agtatatccg cggatctcgg caatggctgg ctgggtgcgc ttgatcatca
     1861 ggatatcgcg atacgttaaa gccttgatac tgaattggag ggtttccagc aggccaaaga
     1921 caatgccctc cagttctttt tccttccggc ttgtgcccac cgtaaccgtc ttggcctgat
     1981 ggcggatggc gtcgatcgga cgggtgagct cgtcgatggc cgttcccagt gtcatgtcaa
     2041 gaagatcata gggagaaaaa agatcctttt cctccttgaa gtcctggcgg atttcctgca
     2101 gcggcaattt gcctgcagcg tattttaaca ggagcatcag atcagtcatg gtcttgccgc
     2161 ccaacgcgcc gaacgcgccg ctttgccggt gagcattaaa cagcgcgtaa aattcgttaa
     2221 tcatgcggcg gagagaaaca tcggcaacaa ggtcaaaaac ggatagattc tcctgccttc
     2281 tttgcgtcaa ttcaaccgac aggcgacccc ggaattcacg gaatatctgc gcttccccat
     2341 cgatggcgct cgccgcgtag taaccccaca gatggccggc cattgtgttg agaataaccg
     2401 gcaaaggcga cggcgcggcc gggatcccaa ccacggcgtc cgcgacctga tcgaaacgat
     2461 gatcacccgc attcgcgaaa acaaccacag ctgctttatg ggccttgaaa atggccacgt
     2521 ccttgacaat atcgtccata accgtttccg gatttccggc ggcgcagaca agaatgaaag
     2581 gttcggcgga caaatcaata tgctttttat tctcgacaat gtccgatgaa atggttttgt
     2641 agcaaagttc acttaacttt atgcgtatct cgtcggctgc cgccttgttt ggcccgctgc
     2701 ctgtaacggc ccagtatttt ttgccgatgg ctttttttac cgtggcggca acggcttgtc
     2761 gtccggagca aatttgatcc atcagccgcg gcgcggactc gagatggcgc aattgaccgg
     2821 ctatttcatc atccgagcgg gttttcagaa gctgggcgaa aaaaagtgcc agcacctgac
     2881 cggcaataat ctgggcataa aatgcttttg tcgaggcaac ggacatttca acatcgcgtc
     2941 cgtcgctcgt ataaaagacc ccgtgtgact tggcggtgat gtccgattgc cggcgattaa
     3001 cgatggaaat gacatgtgcg cccctttccc gtgccatcgc gacggcccgg ttggtgtcgg
     3061 tcgtcgttcc cgattgtgta atcgggataa ccagcgtgtc cgccaggttg tctttcagtc
     3121 cgaaaccgct taattccgac gctacccgga caaaaatatt aagcgtcgcg ccttcgagat
     3181 aatgcgttaa cgcgtcggaa acagattgag cggcgacggc cgccgttcct tgcccgatga
     3241 tcactatgtt tttgatggaa ccgctcccga gtttggcgct gatttctgcc ggcaccacat
     3301 ccgtgcctaa attaaagcta actcgtgaac gggattgatt gtccgcggag atccgatatt
     3361 ttcccctaag agttcttttt acggacaggg atgattcgaa aatttccttg agaaaaaaat
     3421 gcgggtagcc gttgcggtga atgtcgcggg ttgtaatttc agaatcctgc agggaagcat
     3481 cggtcagctt cagaagttcg ccgttgtagt tgcgggcggt aatgttccgg atacctccgc
     3541 cttttttttg atccagaata aaaatctgac cgccatcctc gccgttcatc ttgagaaaac
     3601 gcggcatgac ttccaccagg ccgtaaagct cagacgaaaa catgtattga tcttcattga
     3661 cgccgacgta aatggcctgt ccgctgcctc tgagcgccag gaacattttt cccgcctcca
     3721 ggttgctggt catggcgatg gcgtgcgatc cttcaaaatc attcacggcc aggcggaagg
     3781 cttcctccag attttttcct aattgcagat acttttcaat ctgcagcggg atgattttgg
     3841 tatccgttgt tattgaccgc gcaatgattt caccttgatg ctccaggggt tgccggaggg
     3901 ccgtgtaatt gtcgatatct ccgttcagga caacattgat ttgcgcagag gctccgggat
     3961 acaatggatc attagacgat agaaaacctg ttgtatggtt gttcactgga tgacaatttt
     4021 cttccgttat cgcacctact gaagcccaac gggtatgagt cattgtcgtt tcacaggttg
     4081 tttcttcgtt cgcaaagcat ttcagaacct tatcgttact gatagagctt ctcaagtcgg
     4141 cgacattgcg tcccagttcg ccgataatgg aataagtttt atacgtgaaa atcacacagg
     4201 tgccggcctg cgcatcaccc tgattcgccg aaacaaaaat ggacaggtta aggaggtcac
     4261 ctttctttgt cctctgctca taatcctcgc tcaggccatt ctctctgatc tgccggatga
     4321 catccggcat tgctttttca ttttttaaaa caaatgcaag ctgaattccc gcagaatcgc
     4381 gcccccggac ttcaagacgg tccagcgcgt ttaacagaaa gttgagcttg cggtattttc
     4441 ggaaagccgc cgtcttaacc tcggtgtttt tttcggcgcc cgtcaggtcc cttattttct
     4501 gcagattggc cagaacatcc ctttcaagga tccagcaaat gtctttgaac caaagcatcc
     4561 ggctgttgat gacctccaga tcaaaggaac tgatgagttc cgcccgctct tcgagcaatt
     4621 tctcctcgtt tgttatgaac gccttcattt cggcggcaag atttgacaaa tcatttgccc
     4681 ggtcggcgtg aaaaaaaagc aattcctgca tggcctcaca tttcagatcc gacaacgcca
     4741 tgcccatgtc atggagggca tcatcgccgt ataaataatg ctccgcaatt ttctccccgg
     4801 cggggacatt ctgcaggccg ccattcttca ctttgcccca caggctgttc aggcggcggt
     4861 cggctgtctt ttccgataat tgactcggca aacggcacgt cattaaaccg gcaaaaccac
     4921 agttgagccg accgggaagc accgggaaaa agatgagaga cggcgccttg acatccgctg
     4981 gacgtcggcc gatataaatc cggcacctga aaacgaaaag cagggacttg ctcagaacgt
     5041 gaaatatttt tacgtaaatc atcaatgcct tatgcaaatg ctgtcgctgc attttaaaca
     5101 tgctattttc caaagaaaca aaccattttc tgcgttacaa tatctgaaag cagccgtaaa
     5161 tctttttccc ggacatcaaa cacgcgcacc acatctgcaa acgctttgtc atccggcaat
     5221 aaactggtca agatattcgg gtattttttc atccaggcgg tattgaaaaa gacgtcgctc
     5281 ttttcgggat agatagccat ataaaaaata tccatttcca ccatatcctg gaaaaaatgc
     5341 gtgccaaaag acaaatcagg aatcagcgaa ccgtcctgat acgctatttc gccgatagct
     5401 gttatattgt taatttccga aaacttgacg ggaacaccca tggccggtgt tgtggttccc
     5461 catcgtcccg gtcccagaag aatcgttggc atgacttcgc gcctgccgat ctgtttattt
     5521 aatttgcctg tcagcctggc cacttcatac ttttcggaaa gacttaaacc ggcatactct
     5581 ttggggtcaa tgtagatgat gcgggatatg gcctgataga tgctgccgcc catgaaattg
     5641 gcttcctgtt gcagcagaat ctttgacttg ttgatttttt ccggcagttc tacccggctg
     5701 tagtggccct tggtctggaa gggacggcat tgcagaagat taatctgagt tttaccctga
     5761 gcattaaagt ttacggtaaa ttcaatatcg accggataac gatacacctg ctccagtttt
     5821 ctaagcatgt tggacatggt ctgggcgaag gcggtgtttt ctagcaactc atcgaaggtc
     5881 aggatccagg catctttcgc cggtttgccc agggattgca tgtattcgga tgcggcaaca
     5941 tcgcgtgtcg caatcatgtc caggtgcgct tcgatggatt ccgtcagtaa gtcggctgcc
     6001 ggaagcgttt gaaaattatt ggcttgtaga ttcagaatat ccacctcact ctgggagaat
     6061 cgcttgatgt cctcctgttt agcggaaggc ttggtcagag gcgcatcgag cgcgacgata
     6121 cgaggataat cattttccac gcgattgacc gctcttgtcc ccaggccaaa aacaatgcgc
     6181 agcatgccgg ctttcggatc catacctttt tgccagacga aggtattgta ggacaaaccg
     6241 acgcccgcca gatcagggaa aaaataggta gcgcggtgag aaccggaaac acgttgaacc
     6301 agcagcgcca tctgttcgtc atgctgggcc atgccgcgct gcagtcggta ggtcagggcg
     6361 tcttcgttca tggtggaggc gaaaattttc cggacggttt cttcaaaata tcggtagcgc
     6421 tcttcgggcg ttccctggtt gacgcaaaaa tagctttcgt attttccggc aaaagcgttg
     6481 ccaaaggcat cctccagaag cgaactggaa cgcgcaataa tcggagattg tccgaaatat
     6541 tccagcatga gttgaaattg ctccttgact tcatcgggga atacaccgtg cagcatcttg
     6601 cttttgagct catgcgccac ctcaaaatat ccttcatcgg tcttttgcgc catgagcagt
     6661 ttccaccagc cgttttgaac catgtaggag tagaaaatat ccgagccgat atagaaagaa
     6721 tcgtgtattt ccagctcggc gttccaagca aaggttgtgt cgttgcgcag gatgttccgc
     6781 gcgagcagca taccgacgga tttgccgccg ataaaccccg taccgatcag gcgtttcttg
     6841 atttccagca gatcttccag ggagaaatat tccttcacca gcgacaacat ccttttttcg
     6901 cgtccgatca gcatgcgtga gagcgtttcc accatctcct gcttttcttg aaaggctttc
     6961 ggatcttcca gaatgttcgt ggctttaagg aaaatacgat cccagtaatc aagatttcgt
     7021 gcgccgctgg tagcgttctt gtccgtcagg taagacagag tgctggcggc atccgcactg
     7081 tttaagatcg ggacaaattt atcgccttcc ataatgtgcg ggagaaacat cgttggtgta
     7141 tagcgtttcc atgctttgag cggatggacg cagagtttgc ccttattgtt atagacgtcc
     7201 agcagcacct gcgtggtttc gcgaatccgc gccacggtct tgaaagagtg acggctccgc
     7261 aagatagcga aataggccac cgtatttaat tcaaacaggt aggggcaggt gatgacgaag
     7321 aaattgccga tcatcaggtc cgtcgcccag gcttggagca gatcggacag gcagtcaaaa
     7381 acgtaaaata cgtcgcggcc ctcatcgctg atgattttgt ggacctcggt ggaaaaagat
     7441 tcaaatccgg aattggcgtt cagtgtatag attttgattc cgggccccgc ttccaaaagg
     7501 gcagggtggt tggcgaaacg catatagaca agacgctgat tcatggccaa ggtttcctga
     7561 acataggggg cgacgaattt tttgtaatcc tcaacatcgt caacctggag gacgacattg
     7621 tcgcccatct gcaggtaatt gagaatttta tccaggccgt tgatgccggt gctgactcgg
     7681 gcataagggg tcataaaact ctgtttcctc ttctcatttt caataaaaca aatttgttcg
     7741 gcgcaaatcg gctcgctgga atggtccgcc gatgtcccaa atggcgatca tgttaaaatg
     7801 caaagtatta acaatggttt gcggaacatg actttgcttc aatcaattga tttgccattt
     7861 ggccgccata tttcaatttt ccgccgcaag tggttcttca atttttatgc caccaacgcc
     7921 taaatcacat ttgcgccttt ttcctcccgc tcttttacgg gtgatccgtg gaacattttt
     7981 gaacctatgc caaacttgaa tccagcggag gggaaatttg ccggtctttg cggcataaaa
     8041 aagcagtcaa catgaaaatc actgtttttt cctaatgatg gaaggttttc tgaagcctgc
     8101 cgcggagaat cttccatttt ttaccagaca agtcatttag cccacatttt agttttctgt
     8161 tttaaaaaat tacacattat tgtcataaat ggaacaagca cgtaatcgac aaattatcag
     8221 taatatcaat aactaaaaat aatcttaaaa gatggcactc tctttgcgct agggaaaacc
     8281 gcaacgaaaa tttttaaagg aggtgcacat tatgaattcc ggagatacag cctgggtgtt
     8341 aaccgccagc gccctggttc ttttgatgac cataccgggg ctggtatttt tttacggggg
     8401 tcttgtccgg cgcaaaaacg ttctctccat tttgatgcaa tgcttcatca tcgtctgcgt
     8461 catctccctg cagtgggtgc tgttcggtta ctccctggcc ttcggtccgg actttcacgg
     8521 cctcatcggg aagttggact gggtgggact ttccggcgtg ggcgccgtgc cgaacaaaga
     8581 ttacgccgca accattccgc attcggcttt catgatcttt caggccatgt tcg
//
LOCUS       DAONQZ010000089         8632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_8877_length_8632_cov_15.274455, whole genome shotgun sequence.
ACCESSION   DAONQZ010000089 DAONQZ010000000
VERSION     DAONQZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8877_length_8632_cov_15.274455"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLR13_10520"
     CDS             <1..326
                     /locus_tag="PLR13_10520"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQC19814.1"
                     /translation="CRKAKEFLLQKKVEINERDYFKEPFTRDEIKELLQGKPASDMFS
                     FKSPSFKALGLNPAGLHDKDLMNLMLSEPRLIRRPVVKIGRHVYFGADIKVLSEIITQ
                     PVENG"
     gene            346..819
                     /locus_tag="PLR13_10525"
     CDS             346..819
                     /locus_tag="PLR13_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQC19815.1"
                     /translation="MPKKTSAGIVLYRFVGEDMEVFLVHPGGPFWAKKDKGAWSIPKG
                     LFEPGEDPLEAARREFHEETGHAVSGEFRALMPLKQPSGKVVHAWAVEGHIDEKSIKS
                     NMFSMEWPPRSGKEQSFPEVDRGGWFTLRIACEKILPGQRGFLEELQTKIKNRPA"
     gene            955..1416
                     /locus_tag="PLR13_10530"
     CDS             955..1416
                     /locus_tag="PLR13_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HQC19816.1"
                     /translation="MDQKIICLSVGLSQKEIEKGKALYFAASEGQSTLEVTAMSRSML
                     NMKIIEILACLMEELEKNPAEKQNDSADVIPADHCKAAVLVMLAQDRELVLQVMRSFK
                     AVLSDSRNVIFAVITETALKWTLAEYLEHLSREHEYMQTSRPENNPDMKKL"
     gene            1441..2748
                     /locus_tag="PLR13_10535"
     CDS             1441..2748
                     /locus_tag="PLR13_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HQC19817.1"
                     /translation="MIYNEEYETLPREALEALQLKRLQQVVNRVYHHVGFYKKSFDRA
                     GVHPNDIRTLDDLKRFPFTTKQDLRDNYPFGMFATPMSNIVRLHASSGTTGSSTVVAY
                     TKRDIENWAELVARCFAAADLTRGDIIHNAYGYGLFTGGLGVHYGAEKLGASVIPMSG
                     GNTKWQLMILQDFGPTAICCTPSYALNLAEQGRAMGIDMPSLKLRVGIFGAEPWSEQM
                     RAQIEEAFAIKAMNIYGLSEIMGPGVSMECSEAQAGMHVFEDYFLVETIKPDTGEVLP
                     PGEQGELVFTTLTKEAFPLIRYRTRDISRLITTPCRCGRTFHRMERVMGRSDDMLIIR
                     GVNVYPSQIESVLINIEGLEPHYQLIIERSGTLDTLEVQVEVTEGVFAEAGEVKGLQQ
                     LEKRLIKDMKDYLGITAKVKLVEPKSIMRSEGKASRVIDKRKI"
     gene            2805..3566
                     /gene="hisF"
                     /locus_tag="PLR13_10540"
     CDS             2805..3566
                     /gene="hisF"
                     /locus_tag="PLR13_10540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQC19818.1"
                     /translation="MKPIKIMPCLDMKNGRVVKGVHFVDIREAGDPVENAEFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKNVSKVIRIPLTVGGGIASLEDIDMVLGAGASKVSM
                     NSAAVKNPELIRQAAEKFGKEKITVAIDAKRNAQMPSGFELVVSGGTMPIARDAVAWA
                     KICQEKGAGVILPTSMDGDGTKAGYDIPFTRAIADAVTLPVVASGGAGKLEDFYEAVV
                     QGGATVLLAASVFHFRLLKIRDVKTYLREKGLAIA"
     gene            3578..4189
                     /locus_tag="PLR13_10545"
     CDS             3578..4189
                     /locus_tag="PLR13_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19819.1"
                     /translation="MSKKNNAGFMYLALLGAAITTLCDGVHVYTQTLSYTTPFLFGQA
                     WWVFPGFVLAFLFMGGVYFGLVNRLPLFFAVKKSISGGSLRDVVETATAFALIYVMSG
                     LGSNEPLLLSAVFYGTFVLRLAFSYDRLFLLLLAVLMAFGGMFVESLYAAAGLMAYRY
                     PDVFYVPFWLGGLYVHGAFALREGMRFFVYDRKPGTLISVSPL"
     gene            4405..5535
                     /locus_tag="PLR13_10550"
     CDS             4405..5535
                     /locus_tag="PLR13_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC19820.1"
                     /translation="MNRQNNRFWPPLDPILKTVDLVIEPEELNLLLKMGTDLYTYDQV
                     AALSGINSEKFDAVFDSLKKKAFIGIKNPETSGERYTLHPFVVGWFEGQVSYLIGKPE
                     EKEFAKRYMGFLDSLRKYNFFPLRNIMNVIGKHATVSNQSVGTVRESRNGQGKSKIVV
                     DENIVVPDSGIYPTSSVNDLIMEFGTRSIIGQFKVCMCRQMTANVDQPCRLKMPDDVA
                     CMGFGEGIKTYMKYGYMRRISKEQAFDVIQKARDHGAVHTVFHEMDDTKLPQIGLCNC
                     CWDCCGIFRTYNMGVVPLRYRCYYIARIANSDRCTGCGRCEKFCPTSAINVVAKSVVL
                     DTKKCIGCGQCVHQCARAVVELVEDRRTAFLPMLKTSEARLL"
     gene            5581..6204
                     /locus_tag="PLR13_10555"
     CDS             5581..6204
                     /locus_tag="PLR13_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HQC19821.1"
                     /translation="MPGLKKKQHLPDDLKKCVEFHGHICPGLIYGYRVAQEAIRLMHI
                     GRSVDEEVVAVCENDSCAVDALQVMLGTVAGKGNLLIKNYGKNAFTVMSRVNRKAYRF
                     SRKKQYAYGGSSGRRFKKLERAVASGDASEEEKQELKRLKIDDLLARPFSDIFTTEEV
                     VFDEPGYAPLAPSEPCAVCGEMTMATKMIPLRDGRQVCIPCSEKSKH"
     gene            6268..6567
                     /locus_tag="PLR13_10560"
     CDS             6268..6567
                     /locus_tag="PLR13_10560"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQC19822.1"
                     /translation="MKKIGITILVVLGVLMTSIAYAANLYGIVSDKNGKPVEVKVTLK
                     DDKGAAVGQPVTTDKKGAYAFKDIKPGSYVVVVGAKDEWKIFVGPGETRRDFSLK"
     gene            6591..7619
                     /locus_tag="PLR13_10565"
     CDS             6591..7619
                     /locus_tag="PLR13_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19823.1"
                     /translation="MAERDIQKQEEPTEAPRKKDLWDILQALGPALIAAAVAVIGSVY
                     SYQQAQMTKASEMREVYTKIMTERENSDNNIRASMFQMLFNAMFDKDINSADTPDNVN
                     QIKKRVMFLDVLSRNFDTVDIKPLFEDLDGELTRKMYNDRSYSDGAQEDYFALRSELR
                     RIGRNLAVKQLNALASLPGTMVKTIVLIEDKDKNIMVMQDETTANGNGDTLPVEIKAQ
                     NLDDGKVEIAIDYQKAKASDKGFKAPSFIITFYDMPYIDNSVLDKDMRVGVVLTKYAT
                     TREIDLFRDRIDRRLLKDYEDLKAGGIAKYAELRLIKFPAKYTGHRDRPYLQEILQNL
                     VENEKSVR"
     gene            7716..8558
                     /locus_tag="PLR13_10570"
     CDS             7716..8558
                     /locus_tag="PLR13_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19824.1"
                     /translation="MKKYVPVLLLLIFVFACWACVPVPVPVGGTTTVYDESYGYEEPY
                     GYDGYVDVPVVYGEPMYYAPPVSVTFAFDYFTYEAIGGFVDVVFWRGGNRYHREPWYE
                     QGRRITDRDIRSGKRYHKVRGKEFYNHREKLRSKHNIVHPDSYYKLQRSKQKEQQRRL
                     EEQKKRELEMRKQREKQRLEEDRNRYNREKRELQEKQRRLEEQKKRELEMRKQREKQR
                     LEEDRNRYNREKRELQEKQRRLEEQKKRELKKSKEKEKRLKEHYEELKKEAQENSKPL
                     PPVR"
BASE COUNT         2313 a         1894 c         2447 g         1978 t
ORIGIN      
        1 cctgccggaa agcgaaagag tttcttttgc agaaaaaggt ggaaatcaac gaaagggatt
       61 atttcaaaga gccgttcacc cgcgatgaaa tcaaggaact gctgcagggg aaaccggcct
      121 ccgacatgtt cagttttaaa tccccgagtt ttaaggcgct gggattgaat cctgccgggc
      181 tccacgataa ggacctgatg aatctgatgc tgagtgagcc gcgactgatc cgccgtcccg
      241 tcgtgaaaat aggaaggcat gtttattttg gcgcggacat caaggtgctg agcgaaatta
      301 tcactcagcc ggtggaaaac ggttgatcat ttctggtgat tgcaagtgcc gaagaagacg
      361 agcgcaggga tagttttata tcgatttgtg ggagaggata tggaagtttt tcttgttcat
      421 cccggaggtc ctttctgggc gaaaaaagac aagggggcgt ggtctattcc caaagggctg
      481 tttgaaccgg gggaagatcc gctggaggcc gcccgacggg aatttcatga agaaaccggg
      541 catgcggtgt ccggcgagtt tagggcgttg atgcctttga aacagcccag cgggaaagtg
      601 gttcatgcct gggcggtcga aggacatatc gatgaaaaat ccataaaaag caatatgttt
      661 tcgatggaat ggccgccccg ttcgggaaaa gagcagtcct tcccggaagt ggaccgtggc
      721 ggatggttca cactacgcat tgcctgtgaa aaaatattgc cgggtcagcg cggtttcctt
      781 gaagagcttc aaacaaagat taaaaatcgt cccgcctgag tgatgtctgg cagcgaccat
      841 gtggcggaaa atgaattgac tcatgaataa aatatcttta cctacacaaa cgaaaaagac
      901 gtcatcttct gcccgggtgc atcagactgg ggctttgttg taaaggaaat tattatggat
      961 cagaagataa tctgtctcag cgtggggttg tcgcagaaag agattgaaaa aggcaaggcg
     1021 ttgtattttg ccgccagcga aggccaatcg acgctggagg tgaccgccat gtcccggtcc
     1081 atgctgaata tgaaaatcat tgaaatactg gcatgcctga tggaggagct ggaaaagaat
     1141 cccgcagaga aacaaaacga ttccgcggac gttattccgg cggatcattg taaggccgca
     1201 gtgcttgtca tgcttgcgca ggatcgtgag ctggtgttgc aggtgatgag gagttttaaa
     1261 gcggtattgt ctgattcccg gaatgtgatc tttgcggtca taacggagac ggcgctgaaa
     1321 tggacgctgg cggaatatct tgaacatctc agccgggaac atgaatatat gcagaccagc
     1381 aggccagaaa ataatccgga tatgaaaaag ttgtaaagtg ttttgcagga gaaagaaacg
     1441 atgatttaca atgaagaata cgaaacgctg ccgcgcgaag cgctggaggc attgcaatta
     1501 aagcggttac agcaggttgt gaaccgggtg tatcatcatg tcggttttta caaaaaatcg
     1561 tttgacaggg caggcgtgca ccccaatgat atccgcacgc ttgatgatct gaaaagattt
     1621 ccttttacca ccaaacagga tctgcgcgac aattatcctt tcggcatgtt tgccacgccg
     1681 atgagcaata tcgtccgtct gcatgcttca tcgggaacga cgggcagttc caccgttgtc
     1741 gcctacacga agcgcgatat cgaaaactgg gcggagctgg tggcgcgctg ttttgcagca
     1801 gccgatctga cccggggcga tatcattcat aacgcttacg gctacggtct tttcacaggc
     1861 ggactcggcg ttcactacgg tgcggaaaaa ctcggcgcca gcgtgattcc gatgtctggc
     1921 ggcaatacca agtggcagct gatgattctg caggatttcg gtccgacggc gatctgctgt
     1981 acaccttcct acgcgctgaa tctggcggag cagggccggg cgatgggaat cgatatgcct
     2041 tcgctgaaat tgcgtgtggg cattttcggc gcagaaccgt ggagcgaaca gatgcgcgcc
     2101 cagatcgagg aggcgtttgc catcaaggcc atgaatattt acgggctatc cgaaatcatg
     2161 gggcccggcg tatcgatgga atgcagcgag gcgcaggccg gtatgcatgt ttttgaagat
     2221 tatttcctgg tggaaaccat caaaccggat acgggggagg ttctgcctcc gggcgaacag
     2281 ggagaactgg tttttaccac actgaccaag gaggcgttcc cgctgatccg ttaccggacg
     2341 cgcgatattt cccgcctgat cacgacgccc tgccgctgcg gccggacgtt tcaccgcatg
     2401 gaacgcgtca tggggagaag cgatgacatg ctcatcatcc gcggcgtcaa tgtttatccg
     2461 tctcagatcg aatccgtgct gatcaacatt gaagggttgg aaccccacta tcagctgatt
     2521 attgaacgtt caggcacgct cgatacgctt gaagtgcagg tggaagtgac ggaaggggtg
     2581 tttgccgagg ccggcgaagt gaagggattg cagcaactgg agaaacgtct gatcaaggat
     2641 atgaaggact atctgggcat cacggccaag gtcaaactgg tcgaaccgaa aagtatcatg
     2701 cgttcggagg gcaaggccag ccgggtgatt gataaacgga aaatttaaaa cagtcgtagg
     2761 acattccatt atattatata atgctgttac tggagggaaa cgttatgaag ccgatcaaaa
     2821 tcatgccgtg tctggatatg aaaaacgggc gggttgtcaa aggagttcat tttgtcgata
     2881 tcagggaagc gggcgatccg gtggaaaatg ccgaatttta tcaggccgaa ggcgccgatg
     2941 aactggccat gctggatatt gcagcgactc tggaaaaccg taaaacacgt ctggaatggg
     3001 tgaaaaatgt ttcgaaagtg atcaggattc ctctgaccgt cggcggcggc attgcttcgc
     3061 tggaggatat cgacatggtg ttgggtgcgg gcgccagcaa ggtttcgatg aacagcgcgg
     3121 cggtaaaaaa tccggaattg atccgtcagg cggcggaaaa attcggcaag gaaaaaatta
     3181 cagtagcgat tgacgcgaaa agaaacgctc agatgccgtc cggctttgaa ctggtggttt
     3241 ccggcggcac gatgccgatc gcccgcgatg ccgtggcctg ggcgaaaatc tgtcaggaaa
     3301 aaggcgccgg cgtgattctg cccaccagca tggacggcga cggcaccaaa gccggttacg
     3361 acatcccttt caccagagcg attgccgatg cggtgacgtt gcccgtggtg gcttccggcg
     3421 gggcgggcaa actggaagat ttctacgaag cggtggtcca gggcggtgcg acggtcctgc
     3481 tggcggcctc ggtgttccat ttccgtctgc tcaagattcg ggatgtgaaa acgtatctgc
     3541 gggaaaaggg tctggccatc gcctgatgag aaatgtcatg agcaaaaaaa ataacgccgg
     3601 gttcatgtat cttgctttgc ttggggcagc tataacgacg ctgtgtgacg gggtgcatgt
     3661 ttatacgcaa acgctttctt atacgacccc ctttcttttc ggtcaggcct ggtgggtttt
     3721 tccgggattt gtcttggcct tcctttttat gggcggggtc tattttgggc tagtgaatcg
     3781 tctcccgtta ttttttgctg tgaagaaaag catatccggg ggaagtcttc gtgatgtcgt
     3841 tgaaacggcc acggcctttg ccctgattta tgtgatgagc ggactgggca gcaatgaacc
     3901 gcttcttcta tcggcggttt tctacggaac ctttgttttg cggctggcgt tcagttatga
     3961 ccggctattt ctgcttttac tcgcggttct gatggccttt ggcgggatgt ttgtcgaaag
     4021 cttgtatgcc gctgccggat tgatggcgta tcgttatccg gatgtgtttt atgtgccgtt
     4081 ctggctgggc ggactgtatg tacacggtgc ttttgccctg cgggagggca tgcgattttt
     4141 tgtctatgac agaaagccgg ggacgttaat ttccgtctcc cctttatgat gacagcgttt
     4201 catgcagtgt ccttcggtgc atccggccgg tggcatccga tgatggtaca tcatgatgtt
     4261 gttaaatccg gcggtgacga tcagctgaca atgagataaa acatagtggt gaagatgcgt
     4321 gctggttaga aaacgatgat gaccgaagat aaaaaaacgt cttggcgaaa taagaaaagc
     4381 gatctgcgca ggctcgttct tctgatgaac agacagaata acagattctg gccgccgctg
     4441 gatccgattc tcaaaacggt tgatctggta attgagccgg aagaattgaa tctcctgctg
     4501 aaaatgggga ccgatttata cacttatgat caggttgccg cgctcagcgg cataaacagt
     4561 gaaaaattcg acgccgtctt cgactccctg aaaaagaaag cgttcatcgg aataaaaaat
     4621 cctgaaacgt ctggcgagcg gtataccctg catccattcg tggtcggctg gtttgaagga
     4681 caggtgtctt acctgatcgg caaacccgaa gaaaaagaat ttgccaaacg gtacatgggc
     4741 tttctggatt ctctgcgcaa atacaatttt ttccccctcc ggaatatcat gaatgtcatc
     4801 ggcaaacacg cgaccgtatc caatcaaagc gtcggaacgg tgcgcgagtc cagaaacggt
     4861 cagggaaaat ccaaaattgt cgtggatgaa aacattgtcg taccggattc cggaatttat
     4921 ccgacaagct cggtaaatga tctgattatg gaattcggaa ccagaagtat tatcggtcag
     4981 tttaaggtct gcatgtgccg gcagatgacc gcgaatgtcg atcaaccctg tcgcctgaaa
     5041 atgcccgatg atgttgcctg tatgggattt ggcgagggca tcaaaaccta catgaaatac
     5101 ggatatatgc gccggatcag taaagagcag gcctttgatg tcattcaaaa agccagggat
     5161 cacggcgccg ttcataccgt gtttcatgaa atggatgata cgaaactgcc gcagattggt
     5221 ttatgtaact gctgctggga ctgctgcggt attttccgta cgtataatat gggcgttgtc
     5281 cctttgcgtt accggtgtta ttatatagca agaatcgcaa acagcgaccg atgcacgggc
     5341 tgcggccgat gtgaaaagtt ctgtccgaca tccgcaatca acgtggtggc aaaaagcgtg
     5401 gtcctcgata caaaaaaatg catcggatgc ggtcagtgcg ttcatcaatg cgctcgtgcg
     5461 gtggttgaac tcgttgagga cagacgtacg gcctttttac ccatgctgaa aacatcggaa
     5521 gccaggctgc tgtaggaaaa agttgcgatt caatctcgga aacttcagaa aggatgcgat
     5581 atgccgggtt taaagaaaaa gcaacacctg ccggatgatt tgaaaaaatg cgtggaattt
     5641 cacgggcaca tctgtcccgg cctgatctac ggttatcggg tggcccaaga agcgatacga
     5701 ctgatgcata tcggacgctc ggtggatgag gaagttgtgg ccgtctgtga aaacgattcc
     5761 tgtgccgtgg atgcccttca ggtgatgctg ggaacggtgg cgggaaaggg caatctgttg
     5821 atcaaaaatt acggaaagaa cgcctttacg gtgatgagcc gagttaatcg caaagcttac
     5881 cggttttccc gcaaaaaaca atatgcgtat ggcggctctt ccggacgccg gtttaaaaag
     5941 ctggaaaggg ctgttgcatc cggagacgca tcggaagagg agaaacagga gctgaaacgt
     6001 ctgaaaatcg acgatctgct ggcgcgtccc ttttcggaca tctttaccac ggaagaagtc
     6061 gttttcgacg aacccggtta tgcgccgctg gcgccttcgg agccctgtgc cgtttgcggt
     6121 gaaatgacca tggcaaccaa aatgatcccg ttgcgcgacg gaaggcaagt ttgcattccc
     6181 tgttcggaga aaagtaaaca ttgacatatt cgggaagtat cgctaccgtc ctttgaaaca
     6241 gataaatcaa ttcaaggaga tttcctcatg aaaaaaatag gcattacaat tctggttgtg
     6301 ctgggagtct taatgacgtc gattgcatac gcggccaatc tctacggcat cgtgtccgat
     6361 aaaaacggga agcccgttga ggtaaaagtg acgctcaagg acgacaaagg cgctgccgtg
     6421 ggtcagcctg tgacgacgga taaaaaagga gcttatgcgt ttaaggatat caagcccggt
     6481 tcgtatgtag tggtcgtcgg cgctaaggac gaatggaaaa tattcgtggg ccccggcgaa
     6541 acgcggcgcg atttcagttt gaaataatat ccacggtgag gttttggaga atggctgaac
     6601 gcgacatcca gaaacaagaa gaaccgacgg aggctcccag gaagaaggac ctctgggata
     6661 tcctgcaggc actgggaccg gcgctgattg cggctgccgt agctgtcatc ggctcggtgt
     6721 acagctatca gcaggcccag atgaccaagg cttccgaaat gcgggaggtc tataccaaga
     6781 tcatgacgga gcgcgaaaat tcggacaata atattcgcgc cagtatgttc cagatgcttt
     6841 tcaatgcgat gttcgacaag gatatcaatt ccgcggacac gccggataat gtcaaccaga
     6901 tcaaaaaacg agtgatgttt ctggatgtgc tgtcacgcaa tttcgatact gtggacatca
     6961 aacctctttt cgaagatctg gatggagagt tgaccagaaa aatgtataat gaccgatcgt
     7021 attccgacgg cgcccaagaa gactattttg ccctgcggtc cgaattgcgg cgcatcggta
     7081 gaaatcttgc cgtcaaacag ctcaatgctc tggcatcctt acccggcacg atggtcaaaa
     7141 ccatcgttct gattgaagat aaggataaaa atatcatggt gatgcaggat gaaactacgg
     7201 cgaacggaaa cggtgatacg ctccccgtgg aaatcaaagc gcaaaatctt gatgacggca
     7261 aggtggaaat cgccatagat tatcagaaag ccaaagcttc cgataagggg ttcaaggcgc
     7321 cttcctttat tattaccttc tatgacatgc cttatatcga caacagcgtg ctcgacaagg
     7381 atatgcgcgt gggcgtggtg ctgaccaaat atgccacgac gagggaaatc gatttgttcc
     7441 gcgaccggat cgaccggcgt ctcctcaaag attacgaaga cctcaaagcc ggcggaattg
     7501 ccaaatatgc cgaactgcgg ttgatcaaat ttccggcgaa atacaccggc caccgcgatc
     7561 gaccttatct gcaggagatt ctccagaatc tcgtcgaaaa tgagaaatcc gtcagataaa
     7621 ccgggcgatc aatgatgtcg gatgaaagaa agaaacgata tacaacaaac aagtgtttcc
     7681 agatcccggg atctgatgaa aggaggataa tgtttatgaa aaaatatgtg ccagtcttgt
     7741 tgttgctgat ttttgttttt gcctgctggg cctgtgttcc cgtgcccgtt ccggtaggcg
     7801 gaacgacaac agtttatgac gaatcgtacg gatatgaaga gccgtatggg tatgatgggt
     7861 atgtagacgt tccggtcgtt tatggagaac cgatgtacta tgcgcctccg gtatccgtca
     7921 cgttcgcctt cgattacttt acgtatgaag ccatcggcgg atttgtggat gttgtgtttt
     7981 ggagaggcgg caaccgctat caccgggagc cgtggtatga acaaggaaga agaattacag
     8041 acagagatat acgctccggc aaacgatatc acaaagttcg cggaaaagaa ttttacaatc
     8101 atcgcgagaa actgcgcagc aagcacaaca ttgtccatcc cgattcgtat tacaaactgc
     8161 agcgctccaa acaaaaagag cagcagcgcc gtctggagga gcagaagaag cgggagctcg
     8221 aaatgcgcaa gcagagagaa aaacagcggc tggaagagga tcgcaacagg tataacaggg
     8281 aaaaacgcga gttgcaggaa aagcagcgcc gcctggagga acaaaagaag cgggaactgg
     8341 aaatgcgcaa gcagagagaa aaacagcggc tggaagagga tcgcaacagg tataacaggg
     8401 aaaaacgcga gttgcaggaa aagcagcgcc gcctggagga acaaaagaag cgggaactga
     8461 aaaaaagcaa agaaaaagaa aaacggttga aagagcacta cgaggaattg aagaaagaag
     8521 cgcaggaaaa ttcaaaacca ttaccgccgg tgcggtaaag agcaaattgc gttttttagg
     8581 gagtgtaaat ctaactgtgt aaatggtttt atataagata gataataaaa cc
//
LOCUS       DAONQZ010000090         8074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_9579_length_8074_cov_15.629380, whole genome shotgun sequence.
ACCESSION   DAONQZ010000090 DAONQZ010000000
VERSION     DAONQZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9579_length_8074_cov_15.629380"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLR13_10575"
     CDS             complement(<1..242)
                     /locus_tag="PLR13_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19825.1"
                     /translation="MSTGSAPEHAIKEPKGLSKRIQWLRDYYFQGTERAWNNEYTSWT
                     TGTPWDIIYNEMTFYIVPETYTLLTTLGASYLQAARP"
     gene            709..1698
                     /locus_tag="PLR13_10580"
     CDS             709..1698
                     /locus_tag="PLR13_10580"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQC19826.1"
                     /translation="MDQQIKLIDEKVKAKSEFVEKINKAMAGTIIGQKNIIDRLLIAM
                     LCNGHVLVEGVPGLAKTLIVKTLAAAIDAHFSRIQFTPDLLPSDLIGTMVLNPKTGDF
                     TPRQGPIFANIILADEINRSPSKVQSALLEAMQEHHVTISKETYRLPEPFMVMATQNP
                     IEQEGTYPLPEAAIDRFMLKVKITYPGKEEEVQIIDAALIGEEPKVDKVITPQDIMDT
                     RSLINQIYIDEKLKNYIVDLARASRQPEDFGLNDIKGLIQYGASPRASIYLAQAAKGH
                     AFIRRRGYVTPDDIKTIGMDVLRHRIILTYEAEAEEQSVETIVQKIFDTIEVP"
     gene            1702..2583
                     /locus_tag="PLR13_10585"
     CDS             1702..2583
                     /locus_tag="PLR13_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQC19827.1"
                     /translation="MLDRSIYKKIRQIEIRSHKAVNQLFAGEYRSVFKGQGLEFYEVR
                     EYVAGDDIRLIDWNVSARLGSPYVKKFIEERQQSIILCVDVSASQDFGSQKLSKNEAA
                     AEIAAVLAFSAIKNNDLVGLLMFSDKVEKYIPPKRGRRHVLLVIREILSFQRQSKKTD
                     MAQALRYLSRVNKKSSILFVISDFLAEGYEQPINILSKKHDLIPIVIKDAMEKQLPRL
                     PLVCFEDPESGDLIHLDLRDEQLRQAYRDVATDALNRLRKFFRSLKLDHLELGSDGEY
                     IAALNRFFKARERRIAH"
     gene            2583..3101
                     /locus_tag="PLR13_10590"
     CDS             2583..3101
                     /locus_tag="PLR13_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19828.1"
                     /translation="MPEQLRDIKDVIYIFDWGTFLFFLLIALLLVIAGYFLFKFLKKW
                     RRNRSVTKKEPMMPERPCNDVALEALMKMDPVDYFEKRKIKEFYFEMTDIVRQFLGAN
                     YHIDTMDKTSLEITEELERVERDFDKVRTIDRYFCDCDLVKFAKMRPGLAEMKQKKTE
                     SENIVKECWKKV"
     gene            3105..4100
                     /locus_tag="PLR13_10595"
     CDS             3105..4100
                     /locus_tag="PLR13_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQC19829.1"
                     /translation="MRFQSLWFILLLFTLPFLYRFVVKKKPASLPHPDVIGKLGNRFS
                     QTTVKKRLPLYLRLAVLVLLVLALMRLQYGYVADTSNRNGVDIMVALDVSTSMNAVDF
                     KPDRITVAKNVLKNFIDGRPQDRIGLVIFSGKSYLQSPLTTDHNTVLNYLEQIHTGMI
                     EDGTAIGMALATCVNRLKDSEAKTKIILLLTDGENNAGQIDPETAAKMAQALGIKIYS
                     VGIGDPHGAPIKVKDPWGRWVYARNPDGSYFLTKMNEDGLRQISALSEGGYFMAGDEN
                     KLKEIFSEIDKREKTKFNVKDVFIYNEYFAWFLWPALLLLLLEWAYRRLYLRRLP"
     gene            4097..5077
                     /locus_tag="PLR13_10600"
     CDS             4097..5077
                     /locus_tag="PLR13_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQC19830.1"
                     /translation="MIFGDIVHLWMLLIVIGLMVLEVFLWNRKKKLIAGLFPGELTDL
                     MFKNWNRRKHVIKQGLFALAVLFLILALARPQWGKKTEVAGQMGIDVMIAIDVSRSML
                     AEDLTPNRLENAKNSLNLIIDELAGNRIGLVAFAGSGFISCPLTTDVGAVKIFLKTID
                     TELIPDPGTDIGSAIEESIKAFGKSTNSKVIILLTDGEELTGSAVAAAELAAKEGIRI
                     FAVGIGSLEGATIPVAGGFKKDREGNVILSRANPDLLRILSEKTSGRSFMISQDITGF
                     QKLFAAISTLPRHKLKSSVSYQYQDRFQIFIFLSLLCLVAEMMVSERKNA"
     gene            5070..5792
                     /locus_tag="PLR13_10605"
     CDS             5070..5792
                     /locus_tag="PLR13_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC19831.1"
                     /translation="MLSRILKRDKNIVVLLFMLCLPVMAHGAVELPLRSDLNRGNDYY
                     KKGEYSQAGQIYNKILEKKANDLKARFNLGDALYKEGRYKESQSVFESLTGAAVPKDL
                     RQAAYYNLGNSFFKQEDYKSAIGAYEDALKIDPKDKDAQFNLELAKKMLTMPKQQKDK
                     QQDKDKQNKDQQKDKKQSKDNKSENKDKNKEEQQEQQKQPKPGQMSKEDAMRLLNALD
                     DQEKHKAKKMKAGRGGGDDKDW"
     gene            5776..7635
                     /locus_tag="PLR13_10610"
     CDS             5776..7635
                     /locus_tag="PLR13_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQC19832.1"
                     /translation="MTKTGNLPYNETYDITKFAWWIISLTFAVFCVCSVALAADVTFN
                     ASVDKNSVALDDTVQYTLSVSGNSAGNAPSPTLPKFENLSVVGTSQSSSFSFINGQTS
                     VSKSFLYTLRPEKTGQAHIGQATININGQNYTTEPIDIKVTKAEGKKTQPGPQAGARS
                     RFPNAWNDFDDFFKSPFPSRFPQPQVVKDPVKVDLKASQNTVYVNQQILLTFTLYTRV
                     NFAQSPQYAPPDTTGFLAVNLPTDKKLREVTLNGIKYMAQDFKTALFPATAGNFTIGP
                     ATLVVQLDRFSSSQEIKTDPLKIKVLPLPSEGKPSNFSGAVGDFRLDVTLKQNDIERG
                     QPVQITGKVSGRGNIQTISEPVNSFPAEFKKLSATGKEDMMKDQNGVSGSKSFEIVLI
                     PLKEGKFTLPPFEFSYFDPAKKEYRTLKSRELSVNILPSKVPLPQEYEKSLTEENTKK
                     PPTPITIDWQKIGSTVLAVVTSIYFWLPAIIIIALIIIFVLYRKYQEKLVSDPVKLRQ
                     KRALNIARKRLKKAFHLLKKNELKEFLGEIFNSTAKYLGDKYDFSAAGITTDGLKELL
                     SNKGLPTEAQTQLDNFILECDLLRFTPSSLSKEKAVELAEVAEKLIVTIEKTA"
     gene            7669..7953
                     /locus_tag="PLR13_10615"
     CDS             7669..7953
                     /locus_tag="PLR13_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19833.1"
                     /translation="MNAENNIRQHRFWGETTAFLALFLDNVGTLIFFSAILIFTFNYP
                     ADMILTRMIPGTAVGICIGDLVYTWLALRLRKKTGRTDITAMPLGIDKLN"
BASE COUNT         2294 a         1813 c         1930 g         2037 t
ORIGIN      
        1 ggccgtgccg cctgaaggta ggacgcgccc agcgtcgtga gcagggtgta ggtctccggc
       61 acgatataaa aggtcatttc attgtagatg atgtcccagg gcgtgcctgt ggtccaggag
      121 gtatattcat tattccaggc acgctccgtt ccctgaaaat aataatcgcg gagccactgg
      181 atgcgtttag atagtccctt gggttccttg atggcatgtt cgggcgctga tcctgtactc
      241 ataaaaacct ccattgtgct ttgtttatac gataactcca ccaacaattt tcgccgataa
      301 acttatttga acgtttagcc tttaaccggg tttgtgcacg aaaaatttgt cgcccggata
      361 agatgtatta aattttttta gaataacagg acagggctta aaatatgact ccttttcttt
      421 cattaatgtt ttgttatcac gtcttaaata ttacaatttc ttgaatagat tgcaagaata
      481 aatcacggca acgaatctta tattattgca catttcccgt tctatttttt ttaatcccat
      541 cttgacagac gggggatggc ttcttatatt cttcttgaaa atatcatcat aaaatcatag
      601 atctatccga tcttatcgag gggcaataca tattcaggat taatttaata tttttcgttt
      661 aaccgcgata aaaaatcgtt aaaattattt ttttgagagg aatcaattat ggatcagcaa
      721 ataaagctca ttgatgaaaa agtaaaagcc aaaagcgaat ttgtggaaaa aattaacaag
      781 gcgatggcgg ggacgatcat cggccagaaa aatattatcg accgtctctt gatcgccatg
      841 ctctgcaacg gccatgttct ggtcgagggc gttccgggac tggccaagac cctgattgtc
      901 aagacgcttg ccgcggccat cgacgctcat ttttcgcgta ttcagttcac gcccgatctc
      961 cttccatcgg atttaatcgg cacgatggtt ctcaatccca aaaccggtga ttttactccc
     1021 cgtcaggggc cgatctttgc caatattatt ctggcggatg aaatcaaccg ctctccttcc
     1081 aaggtgcaga gcgcgctatt ggaagccatg caggaacatc atgtaactat ctccaaggaa
     1141 acgtatcgcc tgccggagcc ttttatggtc atggccacgc aaaacccgat tgaacaggaa
     1201 gggacctatc cgctgccgga agcggccatt gaccgcttta tgctcaaggt caaaataacg
     1261 tatcccggca aggaggagga agtacagatt attgacgcgg cgctgatcgg cgaggaaccg
     1321 aaagtggata aggtgatcac gccccaggat atcatggaca cccgcagctt gattaatcaa
     1381 atctacattg atgaaaaact gaaaaattat atcgttgatc tggcacgtgc cagccggcag
     1441 ccggaagact tcggccttaa cgacatcaaa ggcctgattc agtacggcgc ttcgccgcgc
     1501 gcatccattt atctggcgca ggccgccaaa ggtcacgcgt ttatacgccg ccgcggctat
     1561 gtcacgcccg atgacatcaa aaccatcggc atggatgttt tgcgacaccg gattattctg
     1621 acctatgaag ccgaagccga ggaacagagc gtggaaacca ttgtgcagaa aatattcgac
     1681 accatcgagg tgccctaatc catgctggat cgctcgattt acaaaaaaat caggcaaatc
     1741 gaaatccgat cgcataaggc ggtcaatcag ctttttgccg gcgaataccg cagcgttttc
     1801 aaaggacagg gcctggaatt ttacgaagtc cgcgaatacg ttgccggaga cgacatccgc
     1861 ctgattgact ggaatgtcag cgcccgtctg ggatcgccgt atgtcaagaa atttatcgag
     1921 gagcggcagc agagcatcat cctttgcgtg gatgtgagcg cgtcccagga tttcggctct
     1981 cagaaactga gcaagaatga agccgccgcc gagatcgcgg ccgtgctggc tttttccgca
     2041 atcaagaaca atgatctggt cggtcttctg atgttttccg ataaggttga aaaatatatt
     2101 ccgccgaaaa gaggaagacg tcatgtcctg ctggtcatca gggagattct ctcctttcaa
     2161 cgccagagta aaaaaaccga tatggcacag gcgctgcgat atctgagccg tgtgaacaaa
     2221 aaaagctcca tcttatttgt gatatccgat tttctggccg aaggctatga acagccgatc
     2281 aatattctgt ccaaaaaaca tgatctcatt cccatcgtca tcaaggatgc tatggaaaaa
     2341 caactgcccc ggctgccgct ggtgtgcttt gaagatcccg agtccggcga tttgattcat
     2401 ctcgatttac gggatgaaca actgcgtcag gcttatcgtg atgttgctac cgacgcattg
     2461 aaccgcttac gaaaattttt caggtcgctg aaactggacc atcttgaact gggctccgat
     2521 ggtgaataca ttgccgcgct caatcgcttc tttaaggcgc gggaaagaag gatagcgcac
     2581 tgatgccgga acaattaaga gacatcaaag acgtcatcta tatttttgac tggggcactt
     2641 ttttattttt tcttttgatc gctctgctgc tggtcattgc cggatatttt ctttttaagt
     2701 ttttgaaaaa gtggcggcgg aaccgtagcg ttacgaagaa agaaccgatg atgccggaac
     2761 gaccgtgtaa tgacgttgca ctggaggcgc tgatgaagat ggatccggtc gactattttg
     2821 aaaagcgcaa aatcaaagaa ttttattttg aaatgaccga tatcgtgcgc cagtttctgg
     2881 gcgccaatta tcatatcgat accatggata aaacatcgct ggaaatcacc gaggaactgg
     2941 aacgggtgga acgggatttc gacaaggtca gaactatcga tcgctatttt tgcgattgcg
     3001 atcttgtaaa attcgccaag atgcgacccg gcctggcgga aatgaagcag aagaaaacgg
     3061 aatcggaaaa tatcgttaaa gaatgctgga agaaggtgta gaggatgcgg tttcaaagtc
     3121 tctggtttat tcttttatta ttcactctgc cttttttgta tcggtttgtc gtgaaaaaga
     3181 agcctgcgag tctgccgcat cctgatgtga tcggcaaact cgggaacaga ttttctcaga
     3241 cgaccgtcaa aaaaaggctg ccgctatacc tgaggctcgc ggtgctggtg cttctggttc
     3301 tggcgttgat gcggctccag tatggctatg tcgccgacac cagcaaccgc aacggtgtgg
     3361 atatcatggt ggcactcgat gtaagcacca gcatgaacgc ggtagatttc aaaccggaca
     3421 gaattaccgt ggccaagaat gttctgaaaa actttatcga cggccgtccc caggaccgca
     3481 tcggcctggt gattttttcc gggaagagtt acctgcaatc tccgttgacc accgatcaca
     3541 acacggtttt aaattatctg gagcagattc ataccggcat gattgaagac ggcaccgcca
     3601 tcggtatggc actggccacg tgcgtcaacc ggctgaagga ttcggaagcc aagacaaaaa
     3661 tcatccttct gttgaccgat ggcgagaaca acgccggtca gatcgatccg gaaacagccg
     3721 ccaaaatggc gcaggccctg ggtatcaaaa tatactccgt cggcatcggc gatccccatg
     3781 gcgcgccgat caaagtcaag gatccctggg gccgatgggt ttacgcccgc aatcccgacg
     3841 gctcctattt tctgaccaaa atgaacgagg acggattgcg tcaaatcagc gcgttgagcg
     3901 aaggcggcta ttttatggcc ggtgatgaaa acaaactcaa ggaaatattt tccgaaatag
     3961 ataaacggga gaaaacaaaa ttcaacgtca aagatgtttt tatttacaat gaatattttg
     4021 cctggtttct gtggccggca ttgcttctgt tgctgctgga atgggcctat cgtcgtttgt
     4081 atttgaggag gttgccgtga tttttggaga catcgtacac ctctggatgc ttttaatcgt
     4141 catcgggctg atggttctgg aggtgtttct ctggaatagg aagaaaaaac tgatcgccgg
     4201 attatttccc ggtgagttga ccgatttaat gttcaaaaac tggaacagga gaaaacacgt
     4261 catcaaacag ggactgtttg cactggccgt tttatttttg attctggcgc tggccagacc
     4321 gcaatggggt aaaaagaccg aagtcgccgg acagatgggc attgacgtga tgattgccat
     4381 tgatgtttcc agaagtatgc tggcagaaga tcttacgccc aaccggctgg aaaacgccaa
     4441 gaattcactg aatctgatca ttgatgaact ggccgggaat cggatcggcc tggtggcctt
     4501 cgcgggctcc ggttttatca gttgtccgct gaccaccgat gtcggcgcgg tcaaaatatt
     4561 tttaaaaacc atcgacacgg aacttattcc cgatccggga acggacatcg gcagtgccat
     4621 cgaagaaagc atcaaagctt tcggcaaatc gaccaacagc aaggttatta ttcttttaac
     4681 ggacggtgaa gaactgaccg gctcggcggt ggcagccgcg gaactggccg ccaaggaagg
     4741 catcagaatc tttgccgtcg gcataggttc tttggaaggg gcaacgattc ccgtggccgg
     4801 gggattcaaa aaagacagag aaggcaatgt catcttaagc agggcaaatc ccgatctttt
     4861 gagaatatta tcagagaaaa catccgggcg ttcttttatg attagtcagg atataaccgg
     4921 atttcaaaaa ttgttcgcgg caatctcgac actccccagg cataaactca aaagcagtgt
     4981 gtcttatcag tatcaggatc gtttccagat ttttattttt ctgagtttgt tatgcctggt
     5041 tgcggaaatg atggtttcgg agagaaaaaa tgcttagcag aattttaaaa cgtgacaaaa
     5101 atattgttgt cttattattc atgctttgtc tgccggtcat ggcgcatggc gccgttgagc
     5161 ttccgttacg ctcggatttg aaccgcggaa acgattatta taaaaagggc gagtattcgc
     5221 aggccggcca gatttataat aaaattttgg aaaagaaagc caatgacctg aaggccagat
     5281 tcaatctggg agacgcgctg tataaagaag gacgatataa ggaaagtcag agtgtttttg
     5341 aaagtctgac cggcgctgct gtgcccaagg acttacgtca ggcggcttat tacaatctgg
     5401 gcaactcgtt ttttaaacag gaagactata aaagcgcgat cggcgcttat gaagacgccc
     5461 tgaaaatcga tccgaaggat aaagacgcgc aatttaatct ggaactggcc aaaaaaatgc
     5521 tgaccatgcc caagcaacaa aaggataagc agcaagataa agataaacag aacaaagatc
     5581 agcaaaagga caagaaacaa tcaaaggata acaaatctga aaacaaggat aagaataaag
     5641 aagaacaaca ggaacaacag aagcaaccca aacccggtca gatgagcaaa gaagacgcca
     5701 tgcgcctttt aaatgctttg gatgatcagg aaaaacataa agctaaaaaa atgaaagccg
     5761 gacgaggtgg tggtgatgac aaagactggt aatctcccgt ataacgaaac gtatgatatc
     5821 acaaaatttg cttggtggat catcagcctg acttttgctg tgttttgcgt atgcagtgtt
     5881 gctctggcgg cagacgtaac cttcaatgcg agtgtggata aaaattccgt ggcattggat
     5941 gatacagttc aatatacact ttcggtatcg ggtaattcag ccggcaacgc gcccagtccc
     6001 acccttccaa aatttgaaaa tttaagcgtc gtcggcacat cccaatcttc cagtttttcg
     6061 tttatcaacg gacaaaccag tgtgtccaaa tcattccttt atacgctgcg accggaaaaa
     6121 accggccagg cgcatatcgg acaggccacc atcaatatca acggacagaa ttataccacc
     6181 gagccgattg atattaaagt caccaaagcg gaaggtaaaa aaacacagcc cggtccgcaa
     6241 gccggggctc gttccagatt cccgaacgca tggaatgatt ttgatgattt ctttaaatca
     6301 ccgtttccgt cccgtttccc gcaacctcaa gtcgtcaaag atccggttaa agtcgatctg
     6361 aaggcttctc aaaacaccgt ttatgttaat cagcagatcc ttttgacgtt tacgctttac
     6421 acgagggtca attttgctca aagccctcaa tacgcaccgc cggatactac gggattcctg
     6481 gccgtcaatc tgccgacaga caagaaatta cgggaggtga cgctcaatgg catcaaatac
     6541 atggctcagg atttcaagac cgctcttttc ccggcaacgg ccggaaattt tacaataggt
     6601 ccggcaaccc ttgttgtgca gcttgatcgt ttcagctctt cccaggagat aaaaaccgat
     6661 cctttaaaaa taaaggttct gccgttgccc agtgaaggga aaccctccaa cttttcaggc
     6721 gctgtgggtg acttccgttt ggatgtaacg cttaagcaga atgatattga aagaggccag
     6781 ccggttcaaa taacaggaaa agtatcgggg aggggaaaca ttcaaacgat ttccgaaccg
     6841 gtaaattcgt ttccggcaga atttaagaag ctttctgcca ccggcaaaga agatatgatg
     6901 aaagatcaaa acggcgtttc cggcagcaag agttttgaaa tcgtgcttat tcctttaaaa
     6961 gaaggcaaat tcaccttacc gcccttcgaa ttttcatatt ttgatccggc taaaaaagaa
     7021 tatcgcacgc tgaaaagccg cgaattgagc gtgaatattc tcccctcaaa agttcccctg
     7081 cctcaggagt acgaaaaaag tctgactgaa gaaaatacta aaaagccacc gacgcccatt
     7141 acgatcgact ggcaaaaaat cggctctacc gttttggctg tcgtcacatc catatacttc
     7201 tggctgccgg caatcatcat tattgccctg atcatcattt ttgtcctgta tcgaaaatat
     7261 caggaaaaat tggtatccga tcctgtgaaa ctgcgacaga aacgagcgct gaacattgcc
     7321 cggaaaagat tgaaaaaagc ttttcatctg ttaaagaaaa acgagctaaa agaatttctg
     7381 ggggaaatat tcaattccac ggcaaaatat cttggcgata agtacgattt ttccgcagcg
     7441 ggaatcacca cggacggtct gaaagaactc ttgagcaaca agggattgcc aaccgaggca
     7501 caaacacagc tggataactt tattctggag tgcgatctgc tgcgcttcac accgtcatcg
     7561 ttgagcaagg aaaaagctgt tgaactggcg gaggtggcgg aaaaattaat tgtcaccatc
     7621 gaaaaaaccg cgtgacaaat tctgttgccg cacttataag gttacatcat gaatgctgaa
     7681 aacaatatca ggcagcaccg tttctggggg gagaccaccg cttttctcgc tctctttctc
     7741 gataatgttg gaacgctgat tttcttcagt gccattctta tcttcacgtt taactatccg
     7801 gcagacatga ttctgacccg catgattccc ggcactgcgg tcggaatctg catcggcgat
     7861 cttgtctata cgtggctggc gctgcgttta agaaaaaaaa ccggcagaac cgatattaca
     7921 gcgatgcccc tgggtatcga taaattgaat tgaattgaat tgaattgaat tattgattat
     7981 tgattgaatt gataattgat tgaattgaat tgatattgaa ttgataagaa ctcgcttttc
     8041 gctgttggca gtataagaaa cggtcacttg tgtg
//
LOCUS       DAONQZ010000091         8061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_9607_length_8061_cov_15.938796, whole genome shotgun sequence.
ACCESSION   DAONQZ010000091 DAONQZ010000000
VERSION     DAONQZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9607_length_8061_cov_15.938796"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..2012)
                     /gene="dnaK"
                     /locus_tag="PLR13_10620"
     CDS             complement(90..2012)
                     /gene="dnaK"
                     /locus_tag="PLR13_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQC19834.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGDPVVIANQEGNRTTPSIVAISE
                     SGERLVGQVAKRQAITNSENTVYAVKRLIGRKYTSKEVQYDLKIIPYKITEASNGDAQ
                     VTVRGKNYSPAEISAMILTKMKQTAEDYLGEKIKDAVITVPAYFNDSQRQATKDAGKI
                     AGLNVMRIINEPTAAALAYGLDKKKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGEDFDLRVMDFLVAEFKKDQGIDLRNDKMALQRLKEAAEKAKMELSTTMETDI
                     NLPFITADASGPKHLNIKLTRAKLESLVEELINQVVGPCQTALKDAGMSPKDIDEVIL
                     VGGMTRMPRVQQKVKEIFNKEPHRGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPL
                     SLGIETLGGVMTRLIEKNTTIPTKKSQIFSTAADNQPAVSIHVLQGERQMAADNRTLG
                     RFELVGIPPAPRGVPQIEVAFDIDANGIVHVNAKDLGTGKEQSIKITASSGLSDADIE
                     KLVKDAEMHAEEDKRRKELIETRNQADSMVYSVEKNIKEFGDKIDSAEKTKIEDAIAR
                     TKKALEGDDVEAIKKAQDDLMNVSHKLAEAMYAKSAGPQGGPGADGGAQQQSGDQQAS
                     GKKNDDVVDADFEEVK"
     gene            complement(2023..2628)
                     /gene="grpE"
                     /locus_tag="PLR13_10625"
     CDS             complement(2023..2628)
                     /gene="grpE"
                     /locus_tag="PLR13_10625"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQC19835.1"
                     /translation="MKSKKEQSEHSAKGFQAGEHKDKGAAEDRNKEVEALNQKLEEKE
                     NEATANYDRYLRAVAEFDNYKKRALKDKADTIKYANEEIIKDILPFMDSLDRALEQET
                     GDVQTFREGIALIQEQLLCCLKKYGVERIDAAGQDFDPNFHEALMQMESDQHEDNKVV
                     SEMEKGYLLNGRLVRPARVCVCKKTKKQEDDMIEIMEDTGE"
     gene            complement(2657..3241)
                     /locus_tag="PLR13_10630"
     CDS             complement(2657..3241)
                     /locus_tag="PLR13_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQC19836.1"
                     /translation="MEDYIIKIFKESNRVKDNFLNENLGKLANVIEVITSALKAGNKI
                     LLFGNGGSAADAQHLAAEFVNRFIIERPPLPAIALTTDTSVITSIGNDYDFSEIFSKQ
                     VRAIGQPGDIAWGISTSGNSANVLKGLEMAKKMGLVTVAFTGKDGGDIAKIVDFSINV
                     SSNSTARIQETHITAGHAICELVDIKLFQKPDFQ"
     gene            3514..3954
                     /locus_tag="PLR13_10635"
     CDS             3514..3954
                     /locus_tag="PLR13_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQC19837.1"
                     /translation="MFGPNLWRMKRFADSVPELIDLQREINRLFSSVGQAAGADYPAI
                     NVWEKEDSLVVTTELPGMDPDDINISVTGSMLTISGKSKGDPLKAGETYLRQERELGN
                     FQRNVQLPFQVDSPAVEAKYEKGVLNITLPRQKEDLPRKIQITA"
     gene            3972..>4325
                     /locus_tag="PLR13_10640"
     CDS             3972..>4325
                     /locus_tag="PLR13_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQC19838.1"
                     /translation="MSDKDLQKTENTAATERIRNVKTFVPRVDIYETKDALFLIADMP
                     GIDEQTVDVELEKNILTISGRVENGKVKDYRLVFSEYEVGDYERTFTLSDEIDRDKIK
                     ANVKHGVLRLELPKAE"
     assembly_gap    4326..4425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4426..4680
                     /locus_tag="PLR13_10645"
     CDS             <4426..4680
                     /locus_tag="PLR13_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQC19839.1"
                     /translation="DIDVTVTGDSVTIRGEKKEEKEDKDKNYYYMERSYGSFCRVIPL
                     QTEIESGKAEARFKNGILDIKIPKSQSAKAKGTKVSIKSG"
     gene            complement(4746..5597)
                     /locus_tag="PLR13_10650"
     CDS             complement(4746..5597)
                     /locus_tag="PLR13_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HQC19840.1"
                     /translation="MVGTLRKSLKTIEDYKSANPHYTELLDIMADILILREEYLKNMS
                     RPIFHVDENLIAQKMTGGLPLIDLTDKEFDLKRPRDYFYALIQAAEKRIPELEQSISD
                     IVDDPDFDWEKSVRASFDCKVEEADARTAEADREEENNIDLIDLFLEESLRPELEIIA
                     KKYGEMVAKSDWTEGYCPICGKEPKIGEIRKDEEGKRYLFCHQCGFKWYFYRIKCPFC
                     GNDEQQSLAYFEVEGEERYRVDVCNKCWRYIKTVELPKFSDEPNMDVEDIATLHLDMI
                     AYDEGYN"
     gene            6039..6482
                     /locus_tag="PLR13_10655"
     CDS             6039..6482
                     /locus_tag="PLR13_10655"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQC19841.1"
                     /translation="MFRYEFVTQIEIDATAERVWNVLTDLDSFPQWNPMIRWAKGKVC
                     SGERLTVRFEPKGTRGYTFTPKLLIVEPNRQLRWLGYPRIPAFMDTEHYWLIEQITGE
                     KSRLLHGAIIYGLLTPVSRIIFENTSRGPFEQMNHAHKKRAESRE"
     gene            complement(6496..6873)
                     /locus_tag="PLR13_10660"
     CDS             complement(6496..6873)
                     /locus_tag="PLR13_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC19842.1"
                     /translation="MKKFSVIDRWRIPTYDNPNHVKTADLFFNSKKCKQCGTCVKICP
                     GGCIVTDKISKKDLMQDMKKAVKSGVPRVDVLKTGATLCIACFDCGAACPHGAISIKR
                     NFDPKYFYKRLTQLSDMRLPRKY"
     gene            complement(6875..7630)
                     /locus_tag="PLR13_10665"
     CDS             complement(6875..7630)
                     /locus_tag="PLR13_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQC19843.1"
                     /translation="MSSKDDVLSPLGKYEEGMAWNPVEKVIMERRSIRAFKKEPLPDR
                     MIERILEAGRFAPSAGNSQPWKFIVVNSPEILAEMEKDALKIIKFFMFFIDYTRGNIL
                     RRLYILSWVKWIIRILKNKLHPVPFGLMSQIAQGRAPVFHGAPTLILLAEDRRGVASP
                     PTDIGICGQNMVLTAHSLGAGSCWIGLIQVLMYLPKWRKKFGIKYPYKLNECIALGWP
                     NPRVDGMVPREVQLVQWYERGMNDAPRITKQGE"
     gene            complement(7684..>8061)
                     /locus_tag="PLR13_10670"
     CDS             complement(7684..>8061)
                     /locus_tag="PLR13_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19844.1"
                     /translation="FVGRNAEFMRIKGENVSAYEVEHTIQKHPSVLEAAVYAVPSELA
                     EDEIMASVSLVEGHALKEADLVESLKEDLAKFAVPRFVRIVKEFPKTETQRIIKKELE
                     KIGVVAGTYDAQKNQYVGNTKIA"
BASE COUNT         1962 a         2009 c         1761 g         2229 t
ORIGIN      
        1 tattgtatat tatttgagca ttccgcgcga tataaaaaag cccgaaacgg cagcagccat
       61 tccgggcttt ggaaacacta tggaccgcac tatttcactt cttcgaaatc cgcgtcaacc
      121 acgtcgtcgt ttttcttgcc cgatgcctgc tgatcgccgc tctgctgttg agccccgcca
      181 tccgctcccg gacctccctg aggacctgcg gacttcgcgt acatcgcttc cgcgagtttg
      241 tgggatacgt tcatcaggtc gtcctgagct ttcttgatgg cttcaacatc gtcgccttcc
      301 agggctttct ttgtcctggc aatggcatct tcaatcttgg tcttttccgc cgaatcgatc
      361 ttatcgccga attccttgat attcttctcg acagaataaa ccatcgagtc ggcctgattt
      421 ctggtttcaa tcagttcttt gcggcgttta tcttcttccg cgtgcatttc cgcgtccttg
      481 accagttttt caatatccgc gtcgctcaaa ccgctggacg ccgtgatctt aatactctgc
      541 tccttgccgg tccccagatc cttggcattg acatgcacta taccgttggc gtcaatgtca
      601 aaagccactt cgatctgcgg taccccgcgc ggcgcgggcg gaatacccac cagttcaaag
      661 cgccccagcg tccggttatc cgccgccatc tggcgttcgc cctgaagaac atgaatgctc
      721 acagccggct gattatccgc cgctgtggag aaaatctggc ttttctttgt ggggatagtc
      781 gtattttttt cgattaatct ggtcatcaca ccgccaagcg tttcaatgcc cagagaaagc
      841 ggggtcacat ccagaagcag aacatcctta acatcgcccg acaagacgcc gccctgaatg
      901 gccgcgccga tggcaacaac ttcatcgggg ttaacgcctc tgtgaggctc cttgttgaaa
      961 atttccttca ctttctgctg aacgcggggc atgcgggtca tcccgccgac cagaatgact
     1021 tcgtcaatgt ctttgggaga catgccggca tctttcagcg ccgtctggca tggaccgacc
     1081 acttgattaa tcagctcttc aaccagggat tccagcttgg cgcgggtcag ttttatattc
     1141 aaatgtttcg gaccggaagc atccgcggtg ataaacggca ggttgatatc ggtttccatg
     1201 gtcgttgaaa gctccatctt ggctttttcc gccgcttctt tcaggcgttg cagggccatc
     1261 ttgtcgtttc ttaagtcaat gccctggtct ttcttgaatt cggcaaccag aaaatccatc
     1321 acgcgcagat caaaatcctc accgcccaaa tgggtatcgc cattggtcga tttgacttca
     1381 aacacgccgt cgcccagttc cagaatcgag atatcaaatg ttccgccgcc cagatcgaat
     1441 acggcgatct tttcgtcctt ctttttatcc agaccgtagg ccagcgcggc agcggtcggc
     1501 tcattgatga tacgcataac attgagtccg gcaatcttgc cggcatcttt ggtcgcctga
     1561 cgctgagaat cgttgaagta agccggaacg gtaatgactg cgtccttgat tttttcgccc
     1621 agataatcct cggcggtctg tttcattttg gtcagaatca tcgccgagat ttccgccggt
     1681 gaataattct tgccgcgcac ggtgacctgc gcatcgccgt tggaagcttc cgttattttg
     1741 tagggaatta tctttaaatc atactgaacc tctttcgatg tatatttccg gccgatcaat
     1801 cgcttgacgg catagacggt attttccgag ttggtgatcg cctgcctttt ggcgacttgt
     1861 ccgaccagac gctctccgct ttcagaaatg gccacgatgg acggtgtcgt gcggttccct
     1921 tcctgattgg caataaccac gggatccccg ccttccataa tagccacgca tgagtttgtg
     1981 gttcctaagt caattccaat aatttttccc attttttcct ccttattctc ctgtatcctc
     2041 catgatttca atcatatcat cttcttgttt tttagttttc ttgcacacac atactctggc
     2101 gggtctgacc agccggccgt ttaacaaata acctttttcc atttcactga cgactttgtt
     2161 gtcttcatgc tggtcgcttt ccatctgcat caacgcttca tgaaaattcg gatcaaaatc
     2221 ctgaccggcg gcatcaattc tttccacgcc atattttttc aaacagcata aaagctgttc
     2281 ctgtatcagg gcaatgcctt ccctgaaggt ctgaacatct ccggtttcct gttccagagc
     2341 cctgtccaga ctatccataa aaggcaggat gtctttaata atttcttcgt tagcatattt
     2401 aatggtatcg gctttatcct tgagcgccct ttttttataa ttatcaaatt ccgcaaccgc
     2461 acgcaggtat ctgtcataat tggcagtcgc ttcattttcc ttttcttcaa gcttttggtt
     2521 taatgcttca acctctttat ttctgtcttc agcggcacct ttatctttat gctcaccggc
     2581 ttgaaaacct ttagcgctgt gttccgactg ttctttttta cttttcactg caccctcatg
     2641 aatcatgcat gtaatattat tgaaaatcag gtttctgaaa taattttata tccaccagtt
     2701 cacagatcgc gtgacctgct gtaatatgcg tttcctgaat tcgcgcggtg ctgttcgaag
     2761 aaacattgat ggagaaatcg actattttag cgatatctcc tccatctttg ccggtaaaag
     2821 ccaccgtgac aagacccatt tttttggcca tttccagacc cttcaaaaca ttggcagaat
     2881 tgccgctggt gctgatgccc cacgcaatat caccgggctg gccgatcgct ctcacctgct
     2941 tgctgaatat ttcggagaaa tcataatcat ttccgatact tgtaatcacc gatgtgtccg
     3001 tggtcagagc gattgccgga agcggaggtc tttcgataat aaagcggttg acgaattcgg
     3061 ccgcaagatg ttgggcatct gccgctgaac cgccattacc gaacagcaaa attttattgc
     3121 ccgctttcaa tgccgacgtt atgacctcga taacattcgc cagttttccc aaattttcat
     3181 ttagaaagtt atctttgacg cgattacttt ccttaaaaat ttttattatg taatcttcca
     3241 tatttatatc attaaaccag gtgtcaaatt ccgtttcctg aagcggactt aatatattta
     3301 cggggttagg gcatgtcaag ggaatgacaa aaatttctta tttttattca gtagatattt
     3361 tgccacgagc gcagatctta tcgacaatcg gagatggttg cgaatcttga caaataattt
     3421 ttcgtaaata tatttaaagt gaaaacagtg aaataattaa taatagcgaa tagttataaa
     3481 aaaaataatg ttttcatgaa aggaggatgg tctatgttcg gtccaaatct ttggagaatg
     3541 aaaagattcg ccgacagcgt tccggaatta attgatttgc aaagagaaat aaaccggctt
     3601 ttttcaagtg tcggccaggc tgctggcgca gattatcccg caatcaatgt ctgggagaaa
     3661 gaggacagtc tggtggtgac aacagaactg ccgggaatgg atccggacga tatcaacatt
     3721 tccgttaccg gttccatgct gaccatttcc ggaaagagta agggcgatcc ccttaaagca
     3781 ggggagactt atctgcgtca ggaaagagaa ctgggcaatt tccagcgcaa tgttcaatta
     3841 cccttccagg tggactctcc ggctgttgaa gcaaaatatg aaaaaggggt tctgaacatt
     3901 accctgccca gacaaaaaga agatttgccc agaaaaattc aaattactgc ataaaaggag
     3961 gtcgttgaat tatgtccgat aaagatctgc aaaagacaga aaacacggca gccacagaaa
     4021 gaatacgcaa tgttaaaact tttgtgccgc gtgtggatat ttatgaaacg aaagacgctc
     4081 tttttctgat tgccgatatg cctggaatcg atgaacaaac ggttgatgtg gagctggaaa
     4141 aaaacatcct caccatttcc ggacgtgtgg aaaacggcaa ggtcaaagac tatcgccttg
     4201 tcttttccga atatgaagtc ggcgattacg aaaggacatt cacactgtca gacgaaattg
     4261 acagagacaa aatcaaggca aacgtgaaac acggtgtatt aaggctggaa ctgcccaaag
     4321 cggaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat tgatgtgacg
     4441 gttaccggcg attctgtgac catcagggga gaaaagaagg aagaaaagga agacaaggat
     4501 aaaaactact attacatgga aaggtcatac ggttcattct gcagggtgat tcccctgcaa
     4561 acagaaatcg aatcgggcaa agcggaggcg cgttttaaaa acggaattct ggacatcaag
     4621 attcccaaga gccagagtgc caaagcgaaa gggaccaagg tttccatcaa gagcggctaa
     4681 tctgcaaagc ccgcctcttt tcccaggggc gggcttttcc agaatcttaa acattttctt
     4741 ggggcctaat tgtacccttc gtcgtaagca atcatatcca gatgcaacgt ggcaatatct
     4801 tcaacatcca tgttgggttc gtcgctgaat ttgggcaact ccactgtttt gatgtagcgc
     4861 cagcacttgt tgcagacatc cacccggtag cgctcttcac cttccacttc aaaataggcc
     4921 agtgattgct gttcgtcgtt gccgcagaaa gggcatttga tgcggtagaa ataccatttg
     4981 aacccgcact gatggcagaa cagataccgt ttgccctctt catcttttct gatttcaccg
     5041 atcttcggtt ccttgccgca aatcggacag tagccctccg tccagtcgga tttcgcgacc
     5101 atctctccgt attttttggc gatgatttcc agctccggcc tgagactttc ttcgagaaaa
     5161 agatcgatca gatcaatatt gttttcttct tcccggtccg cctccgccgt ccgggcatcg
     5221 gcttcttcaa ccttacagtc aaaggacgcg cgaacacttt tctcccagtc gaaatcggga
     5281 tcatcaacga tatcggaaat gctttgctcc agttcaggta tgcgtttttc cgccgcctga
     5341 atcagggcgt aaaaatagtc ccgggggcgt tttaaatcaa attccttatc cgtcagatca
     5401 atcaagggca gtccgcctgt cattttctga gcaattaagt tttcatcaac atggaaaatg
     5461 ggacgggaca tgtttttgag atactcttcc cttaaaatca gaatgtcagc catgatgtcc
     5521 aggagttccg tataatgagg attggctgat ttataatcct caatcgtttt taaagatttg
     5581 cgcagtgtac cgaccatgct ttcatactcc tgtgagacgt ttattaagat ttgttatcct
     5641 gaatgtcact gaataatccg attttcatta gaacaaaact gatcgaccta tatccacttt
     5701 cccatgtatc ttcaagtagt tttttcacag gcaacaaaac tgcggcggcg acatcaactc
     5761 ccggataaaa gcatccataa gtatccggat atcctatgga tatcttttga caagacttct
     5821 gcaaatcatg acaaaatgct gccggcggca tccgacggca aataaaagcc gaacgatgcg
     5881 caaaaacgcg ctctgtcggc aaaaaactcc agccatccgg ccgggtctat ttcatctttg
     5941 ggtttaacgt gccatttttg cctgcggctt taatccactt gacacacagg ataccgtctc
     6001 atatagtcca gactcgctaa aaggaatgtt tatcataaat gtttcggtat gaatttgtaa
     6061 cacaaattga gatcgacgcg acagcggagc gagtatggaa cgtcctcacc gacttggatt
     6121 cctttcccca gtggaatccg atgattcgct gggcaaaagg aaaagtatgc tcgggagaga
     6181 ggctgaccgt ccggtttgaa ccgaagggaa ccaggggata tacttttacg ccaaagctgc
     6241 tgatcgttga accgaaccgg caactgcgct ggctgggata cccccggata cctgcattca
     6301 tggataccga acattactgg ctcatcgaac agataaccgg cgaaaaatcc cgtctgttgc
     6361 atggggctat catttacggc ttgctcacac cggtgagccg gattattttt gagaacacct
     6421 cccggggtcc ctttgagcag atgaaccacg ctcacaaaaa acgcgccgaa tcacgtgaat
     6481 gaatagtgat ccggattaat attttcttgg caacctcata tcagacaact gagtcagcct
     6541 cttataaaaa tatttcggat caaaatttcg tttgatggaa atggcgccgt ggggacaggc
     6601 ggcgccgcag tcgaaacagg cgatacaaag cgtggctccc gtctttaaga catccacacg
     6661 aggcaccccg ctttttacgg cctttttcat gtcttgcatg agatctttct tggaaatttt
     6721 gtcggtaaca atgcatccac ccggacatat cttgacacat gtgccgcact gcttgcattt
     6781 tttagaatta aagaataaat ccgccgtttt cacatgattc ggattgtcat aggtcggtat
     6841 ccgccatcga tcgatcacgc tgaatttttt catatcattc cccctgcttg gtaattcttg
     6901 gagcgtcgtt catgcctctt tcgtaccatt gaaccaactg cacctcgcgc ggcaccatgc
     6961 catcgaccct cgggttgggc caaccgagag cgatacattc gttgagctta taaggatatt
     7021 tgatgccgaa tttttttctc catttaggca gatacatcaa tacctgaatg agaccgatcc
     7081 agcagcttcc cgcccccagg ctgtgcgcgg tcaggaccat gttctgcccg caaattccga
     7141 tatcggtggg cgggctggcc acaccccgtc tgtcctccgc cagaaggatc aacgtcggcg
     7201 cgccgtgaaa gacaggcgcc cggccctggg caatctgcga catcagcccg aacggaacag
     7261 gatgaagttt gtttttcaaa atccggatga tccatttgac ccagctcaga atatagagtc
     7321 ggcgcagtat attgccccgg gtgtaatcaa tgaaaaacat gaagaacttt atgattttca
     7381 gcgcgtcttt ttccatttct gcaagaattt cagggctgtt gacgacaatg aatttccagg
     7441 gttgtgaatt tccggctgac ggggcaaacc ggccggcttc cagaattctt tcgatcatgc
     7501 ggtccggcaa cggttctttt ttaaaagccc ggatgctgcg ccgctccatg atgacttttt
     7561 caacgggatt ccaggccatg ccttcttcgt attttcccaa cggagacaat acgtcatctt
     7621 tcgaactcat atttcctcct tacggttgat ttactgcaaa ccttttgaga cgtccttgaa
     7681 acgttatgca atcttcgtgt tacccacgta ctgatttttc tgggcgtcat acgttcctgc
     7741 cacaacgccg atcttctcca gttctttttt gatgattcgc tgtgtttcgg ttttgggaaa
     7801 ttctttgacg atgcggacaa aacgcggaac ggcgaatttg gccagatctt ccttgagtga
     7861 ttcaaccaga tcggcctctt tcagcgcgtg cccttccacg agagaaacgc tcgccatgat
     7921 ctcatcctcc gccaattccg acggaacggc gtaaacggcc gcctccagca cggaaggatg
     7981 tttctgaatg gtatgttcga cttcgtacgc ggaaacattt tcgcccttga tgcgcatgaa
     8041 ctccgcgttg cgtccgacaa a
//
LOCUS       DAONQZ010000092         7332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_10833_length_7332_cov_16.265631, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000092 DAONQZ010000000
VERSION     DAONQZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_7332_cov_16.265631"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLR13_10675"
     CDS             complement(<1..285)
                     /locus_tag="PLR13_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQC19845.1"
                     /translation="MKTKIAELFGIQYPVILPGMSWISVPELVAAVSNAGGIGYLATG
                     PLSPAKTRQSIKRIRELTDKPFGVGATLLMPGAYENALVAIEEKVPVLNIS"
     gene            354..1073
                     /locus_tag="PLR13_10680"
     CDS             354..1073
                     /locus_tag="PLR13_10680"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQC19846.1"
                     /translation="MAYKLDRSIYFVKNYFLLKIASQGKMLDRNYQTGLFIIIIYWRG
                     MFMKTDKKQELIAIGAELMHLKGYNDTGVQEILGKAGLPKGSFYNYFKSKEDFGLLVV
                     DYYMAFYNKIFAKFMDNKSVPPLERIRRLYKSFNKSFRANHYTLGCPIGNFAQELGDT
                     SPVFQARLKKSADTIVRYFSDVLREAQASGHLSRSLDADKTAHYIFNSWEGAIMSMKV
                     EKSLRPLEINEQFLFDYILKP"
     gene            complement(1107..1910)
                     /locus_tag="PLR13_10685"
     CDS             complement(1107..1910)
                     /locus_tag="PLR13_10685"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQC19847.1"
                     /translation="METKLFFYTGTGNSFWTAKKLSLLLHDAETVSMALNLSGRIIAD
                     AEKIGFVFPVHIWGIPAPVIDFLNRLDMDPEKYYFAVAVNAGQVAATLIQLEKLLQQK
                     HVKLSSGFSVCLPSNYIPWGGAEAPAKQKKKFSATLDKINCIASSVRAKEEKPPERGP
                     FWQNILFSAIYRMTFERVPRMDKPFYADAKCTSCGICEKICPAHNIDLVDGKPAWQHR
                     CEQCFACLQWCPEEAIQYGKGTVKRKRYRHPEITLKEMLACVPGKNRLS"
     gene            complement(1920..2384)
                     /locus_tag="PLR13_10690"
     CDS             complement(1920..2384)
                     /locus_tag="PLR13_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQC19848.1"
                     /translation="MKKYVFLSVLFSVFLCQTATADFYKWVDEKGEIQITDYPPPENK
                     AVKNIEIHEVGSEDPLNAEGEDDASKAKNQKKPEIVLYTKNNCPDCEKAKEFLNSKNV
                     PFIEYNMDTDEKAVAKRKEVDQGDEVPFAIINRNQVYGFSESVYNKVLQMQP"
     gene            2637..3779
                     /locus_tag="PLR13_10695"
     CDS             2637..3779
                     /locus_tag="PLR13_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQC19849.1"
                     /translation="MFGKPVSLFSVFGFEIKVDMSWLILAALITWSLASGLFPEYYKD
                     LPKSAYWWMGIAGAIGLFLSIIVHELTHSIVARHYGVSMKEITLFIFGGVAHMEEDPS
                     SPKAEFMIAVVGPVSSFLLAASAFLLYKVGVYSGWPVTLTGVLNYLSWLNMILAVFNL
                     IPAFPLDGGRILRSALWKWKKDIRWSTNIAAQIGSGFGLMLIIMGIFYIVKGNFVGGL
                     WWFLIGLFVRAAAQSSYRQILARNLFHAKKVRDLMVTNPVTVPRAISLEDFIRDYVYK
                     YHFQAYPVVSFGRLTGCIFLKQVASIPREQWAQHTVGSVAVPCSKEITIGPDEDANKA
                     LDLMNRTGNSRLLVVQGDELEGILSLKDMLALLSLKMELNDMEKKT"
     gene            4104..4742
                     /locus_tag="PLR13_10700"
     CDS             4104..4742
                     /locus_tag="PLR13_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC19850.1"
                     /translation="MANDTLLEPRKIPSQERSRKTVAAIYEAATDIFVNTGYAEATTD
                     QIAEKAGVSIGTLYNYFPGKEAILYGLWEKYEKEIKVITDQADQDIRKKGSFDRSIVP
                     VLLNLALELVSYERVQNRLFISQIGLPETIIQKRRELGLYMEATMEAVFRDFSNVRVR
                     NPKIGVHIIWATVQAVFHDYIISVSDEIKPEDFIDELGDMMSRYIFADEAQE"
     gene            5005..5418
                     /locus_tag="PLR13_10705"
     CDS             5005..5418
                     /locus_tag="PLR13_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19851.1"
                     /translation="MFGTLITTHGNLGDELIKSAELIKGRLENIMHICIDQTKDVEDL
                     KKEISNAIKKLDKGNGVLILTDLFGGTPSNISLSFMKEGKVEVLTGVNLPMMLKLSEA
                     KGNMTLRDFACLVKSYGEKNIMLASEILSKKANRS"
     gene            5494..6003
                     /locus_tag="PLR13_10710"
     CDS             5494..6003
                     /locus_tag="PLR13_10710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HQC19852.1"
                     /translation="MAKDLNIALVRVDSRLVHGQILEAWVPFIKAKCIIVVNDKVAAD
                     PFLETVIKMAVPSDIEVIISSLDDFVRNYSFAHGSGKKTIVLFGSIADACHAFEHGFR
                     FDRLNIGNVYNNEYKVCCSSSVFLCDQEIIRLEDLLKETGVFVELQRVPKGRAVNIKE
                     ALKDYWRVK"
     gene            6134..6829
                     /locus_tag="PLR13_10715"
     CDS             6134..6829
                     /locus_tag="PLR13_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HQC19853.1"
                     /translation="MFQEIILTAFCGSLLCLDRVFMQAMISRPVVIAPLMGLLLGNLY
                     AGLIIGAIIELIWIDRLPIGTYIPPNDSLTAVLAVAIALISGSATGGVTPQIIALAVI
                     LAIPFGSLAKQMDVMIIQSNNVLSDQALRDAQENNLRAIERKNYLGLLKVFFFYGVFL
                     LAAQAVVIPLVIWGYPRLNEPLLKALSFTYYFLPLLGIAVALNMLKLRNAVPVICAVF
                     LVVSMLLEFFHVL"
     gene            6819..7307
                     /gene="rimI"
                     /locus_tag="PLR13_10720"
     CDS             6819..7307
                     /gene="rimI"
                     /locus_tag="PLR13_10720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQC19854.1"
                     /translation="MSCKEISLPLTIDPMKKSDLPEIMKIERESFLSPWTVGMFKREI
                     SSDHSHCLCARIHSDGKEIVAGYIVFWLIIDEAHLHSLAVKKEFRGKGIAFRLMQSMK
                     TIAGKNDVLFQTLEVRASNVEAIKLYRKCGFVVKGVRPLYYSDTHEDALIMWADINPV
                     SE"
BASE COUNT         1810 a         1624 c         1792 g         2106 t
ORIGIN      
        1 agatatgttg agaaccggga ctttttcctc gatggcgacc agcgcgtttt catatgcgcc
       61 gggcatcaga agcgtcgcgc ccacaccgaa gggcttgtcc gtcaattctc tgattcgttt
      121 aatggactgc cgtgtttttg ccgggcttaa aggtcccgtg gcgagatagc cgatgcctcc
      181 ggcattggag accgcggcaa ccagttccgg cacgctgatc cagctcatgc cgggaaggat
      241 aaccggatat tggatcccaa acaattcggc gatttttgtt ttcataaatt gtccttttaa
      301 aataataatt tttaattata aaaaatcgaa gcaagactac aatgcttctt ttcatggcgt
      361 acaaattaga ccggtctatt tattttgtca agaattattt tcttctcaaa atagcgtcgc
      421 aggggaaaat gcttgacaga aattatcaga ccggtctatt tatcatcatc atttattggc
      481 ggggaatgtt tatgaagacg gataaaaaac aggagctgat tgccattggc gcggagctca
      541 tgcatctgaa gggctacaat gacacgggcg ttcaggagat tctcggtaag gccgggcttc
      601 ccaaaggttc tttctataat tattttaaga gcaaagagga cttcggactg ctggttgttg
      661 attactatat ggcattctat aataaaatct ttgccaagtt tatggacaat aaatccgttc
      721 cgcctctgga aagaatccgg cgcctttata aatcgttcaa taaatcattc cgggctaatc
      781 attatacgtt gggatgtccg atcggcaact tcgctcagga gcttggagac acaagccctg
      841 tgtttcaggc caggcttaaa aaatccgctg ataccatcgt caggtatttt tccgatgtgc
      901 tgcgcgaggc tcaggcatcg gggcatctct cacggtctct tgatgcggat aaaaccgcgc
      961 actatatctt taacagctgg gaaggcgcca ttatgagcat gaaagtggag aaaagcctcc
     1021 gaccgctgga aatcaacgag cagtttcttt ttgattacat tctcaaaccg tgagatacta
     1081 aaactgtata tcggaaaagt cgtcgattaa gataatctgt ttttccccgg gacacaggcc
     1141 agcatctcct tcagagtgat ttccggatga cggtatcgtt ttcttttgac ggtacccttg
     1201 ccgtattgaa tcgcttcttc cgggcaccat tgcagacagg caaagcactg ttcgcaacga
     1261 tgctgccatg cgggtttccc gtcaacaaga tctatattgt gagccggaca aattttctca
     1321 cagattccgc aactcgtgca ttttgcatcg gcatagaagg gtttgtccat tcggggaacg
     1381 cgttcaaacg tcatgcggta gatggcggaa aaaagaatat tctgccagaa ggggcctctc
     1441 tccggcggtt tttcttcttt ggcccggacc gatgaggcga tgcagttgat tttatccagc
     1501 gtggcgctga atttcttctt ctgctttgcc ggagcttcag cgcctcccca gggaatgtag
     1561 ttgctgggca ggcaaaccga gaatcccgaa gacagtttca catgcttctg ttgcagcaat
     1621 ttttccaact ggatcagcgt tgcggcaacc tggccggcgt tgaccgccac ggcaaaataa
     1681 tatttttccg gatccatgtc cagacgattt agaaaatcaa tcaccggtgc aggaattccc
     1741 cagatatgaa ccggaaaaac gaaaccgatt ttttcagcat cggcaattat cctgccactg
     1801 aggttaagtg ccatggaaac ggtttcagcg tcatggagca gcagagaaag tttttttgcc
     1861 gtccagaaag aatttcccgt tccggtatag aaaaatagtt ttgtttccat tgagccacat
     1921 caaggctgca tttgcaggac cttgttataa acgctttccg agaatccgta tacctgattg
     1981 cggttgatga tcgcgaaggg cacttcgtcg ccctggtcca cttcttttct tttcgcaacg
     2041 gccttctcat cggtatccat gttgtattca atgaagggaa cgtttttgga attcaagaat
     2101 tctttggctt tttcgcagtc cggacaattg tttttggtat aaagcacaat ttccggtttc
     2161 ttctggtttt tggccttcga tgcatcatct tcgccttcag cgttcagtgg atcttcggag
     2221 ccgacctcat gaatttcaat gttttttaca gccttatttt ccggcggtgg ataatcggta
     2281 atttgtattt ctcctttttc atcaacccac ttataaaaat cagccgttgc cgtctgacac
     2341 agaaaaacgg agaataaaac cgataggaag acatactttt tcataacctg acctcacgat
     2401 aagctgtttt aaaaaatagt gattcttttc cggccggctt aatcaggctt tcatcaaacg
     2461 tcacgatcaa accggttaca atataattaa aagtatctta aatcatccgg cgttgtcaaa
     2521 ttctttatcg ttgcgtcttg cggggctgga ggagagcatt ccggatgctt ctgttaaaaa
     2581 tattgatttt catctttctt ctatattata tgaaacacat tctataagga gcatttatgt
     2641 ttggaaaacc ggtaagcctt ttttcagtgt ttgggtttga aataaaagtc gatatgagct
     2701 ggctgatttt ggcggcgttg atcacctggt cgctggccag cggtcttttc ccggagtatt
     2761 acaaagacct tcctaaatcc gcctattggt ggatgggcat tgccggcgcc ataggccttt
     2821 ttttatccat tatcgtgcat gaacttaccc actccattgt tgcccgtcat tacggtgtat
     2881 cgatgaagga aataaccctt tttattttcg gcggtgttgc gcacatggaa gaagatccgt
     2941 ccagtcccaa agctgaattc atgattgccg tggtcgggcc tgtgtccagc tttctgctgg
     3001 ccgcctctgc ttttttgctt tacaaggtcg gggtctacag cgggtggccg gtgactctga
     3061 cgggggttct gaattatctg tcctggctga acatgattct ggcagtattc aatctgattc
     3121 ctgcttttcc tttggacggc ggacgaatcc tgcgttccgc gctatggaaa tggaagaaag
     3181 acatccgctg gtcgacgaat attgccgctc agatcggttc aggcttcggc ttgatgctga
     3241 tcatcatggg cattttttat atcgttaagg gaaatttcgt cggggggttg tggtggtttc
     3301 tgatcggttt gtttgtccgc gcggcggcgc aaagttccta ccggcagatt ctcgcgcgga
     3361 atcttttcca tgcgaagaaa gtcagggatt tgatggtcac caatccggtc accgtaccgc
     3421 gggccatatc cctggaggat tttatccgcg attatgttta caaatatcat ttccaggcgt
     3481 atcccgtcgt ttcgttcggg agattgaccg gctgtatctt tttaaaacag gtggcctcta
     3541 tcccgcgtga acaatgggct cagcataccg tggggtctgt ggctgttcct tgcagcaagg
     3601 agatcacaat cggtcccgat gaggatgcga ataaagccct tgacctgatg aatcgtaccg
     3661 gcaacagccg tttgctggtt gttcagggag atgaacttga ggggatcctt tcgctgaagg
     3721 atatgcttgc gctgctgtct ttgaaaatgg aattgaatga catggaaaaa aagacatgag
     3781 cccttttttg tccgaatgca gcaaccacag atcagacgat cggcgatcat tttatcacat
     3841 aatatttttt ttcgatgaag attcattacg atcgtgaccg tgcgcgctcc ctcttcaaca
     3901 tggatctgtg gtcgacgaag atttcttgca cagaatggtt tcacggtgca tgacccgtca
     3961 gtgggaattg agcggcgctt ttggcaggtc ctgaaggatt ctttgaaaat ggcgatgatc
     4021 ttttcagctt gactgttcaa gtaagttttt ttataagttc ttacagcttc atcgtcttac
     4081 aaagcttccc ggaaggactg tttatggcca acgatacatt gcttgaaccg cgcaaaattc
     4141 catctcagga acgctcgcgt aaaacggtag cggccatcta tgaagctgcg acggatattt
     4201 ttgtcaatac ggggtacgcg gaagcgacca cggatcaaat agctgaaaaa gcgggcgttt
     4261 cgataggtac tttgtataac tatttccccg gcaaggaggc tatattatac ggtctttggg
     4321 aaaagtacga gaaggagatc aaagtcataa cagaccaggc cgatcaggat atccgaaaaa
     4381 aaggatcttt tgatcgcagc attgttccgg ttctgcttaa tcttgctctg gaactggtca
     4441 gttatgaacg cgtacagaat cgactgttca tcagccagat cggtttgccc gaaaccatta
     4501 ttcaaaaacg ccgggagctc ggtctttaca tggaagcgac catggaagcg gtgttccgtg
     4561 atttttccaa tgtccgtgtc cgaaatccga aaatcggggt acatattatc tgggcgacgg
     4621 tgcaggctgt ctttcacgat tatatcatct ccgtttccga tgaaatcaaa cctgaagact
     4681 ttattgatga actgggcgac atgatgagcc gctatatttt tgccgacgaa gcacaggaat
     4741 aattgcggcg gatttcggga ttcgatcggc tggatcttaa cggtattatt tcacaacctc
     4801 ctgaagatcg agcagttccc caggccctct tgaatgataa attctttctt tccggtgcgg
     4861 tgaatttccg accgatacag cactaaaaaa ttatttccat aaaccttgac aattatggtg
     4921 caatccatta gcgtaacgct ttaaattcga aggcaaagac gcggtcatga tcattgcgta
     4981 aacgggttga aaagggggtt aataatgttt ggcacactta ttaccacgca tggcaatttg
     5041 ggagatgaat tgatcaaatc ggctgaactg atcaaaggtc ggcttgaaaa tataatgcat
     5101 atctgcattg accagacgaa agacgtcgaa gatttgaaaa aagaaataag caatgccatc
     5161 aaaaagctcg ataaaggcaa tggcgtattg attctgaccg atcttttcgg ggggaccccg
     5221 tccaatattt cgctttcgtt catgaaagaa ggtaaagtag aggttctcac cggcgtcaac
     5281 ctgcccatga tgcttaagtt gtctgaagcg aaggggaata tgacgctgcg cgattttgcc
     5341 tgcctggtga agagttacgg cgaaaaaaac attatgctgg ccagtgaaat tttaagcaaa
     5401 aaagccaaca ggtcatgaaa tccacgccct tttacccgca gatggccgcg ctattgaaaa
     5461 gggagtctta ggattccgcg acagggggaa aacttggcaa aagatctgaa tatagctttg
     5521 gttcgtgttg atagccggtt ggttcacggt cagattttag aagcctgggt gcccttcatt
     5581 aaggcaaaat gcatcatcgt cgttaacgat aaagtggccg cggatccttt tcttgaaaca
     5641 gtcatcaaaa tggcggtgcc cagcgatatc gaagtcatca ttagttccct cgatgatttt
     5701 gtccggaatt attcttttgc tcacggcagc ggaaagaaaa cgatcgtttt gttcggcagc
     5761 atagccgatg cgtgtcatgc ttttgaacat ggtttccgtt tcgaccgatt gaatatcggc
     5821 aacgtttata ataacgaata caaggtatgc tgctcctctt cggttttcct gtgcgatcag
     5881 gaaattattc gtctggagga tttgctgaaa gaaacaggag tttttgtcga attgcagaga
     5941 gtgccgaaag gaagagcggt caatattaaa gaagcgctga aggattattg gcgtgtaaag
     6001 taagcagcgc atttgctcat atgatatgct gctttaaatt ctttgtgtct tttttcgcgt
     6061 agagggtttt tgtgttggtt gcggctgtac cggtccgcaa tgcgtttctg ttcgaaaatc
     6121 tggggcgagt gacttgtttc aagagattat tttaacggca ttttgcggca gcctgctgtg
     6181 tctggatcgc gtttttatgc aggccatgat ttcccgcccg gtggtgattg ccccgctcat
     6241 ggggctattg ctgggaaatc tgtacgcggg actgatcatc ggagcaatca ttgaactgat
     6301 ctggatcgat cgtctgccga tcggaacgta tattcccccg aatgattccc tcacggcggt
     6361 gctggccgtg gctatcgctt taatctccgg ttcggcaaca gggggtgtta ccccgcaaat
     6421 catcgcgctt gccgtcattc tggccatccc ctttggcagt ttggccaaac aaatggacgt
     6481 catgattatc cagtccaaca acgtcctttc tgatcaggcc ctccgggacg cgcaggaaaa
     6541 caaccttcgc gccattgaac gaaaaaatta tctggggctt ttgaaggtat ttttctttta
     6601 cggtgttttt ctgcttgccg ctcaagcggt tgtgatcccg ctcgtcatct ggggatatcc
     6661 tcgcttgaat gaaccgctcc tgaaggccct ttccttcacg tattattttc tgccgctttt
     6721 gggaattgcc gtggccctga atatgcttaa attgcgcaat gccgttcccg tcatctgcgc
     6781 cgtattttta gtcgtgtcca tgcttctgga atttttccat gtcttgtaag gaaatttctt
     6841 tgccgttgac cattgatccc atgaagaaat ccgacctgcc ggaaatcatg aagatcgagc
     6901 gggaatcgtt tttgtctccc tggacggtgg gcatgttcaa gagagaaatc tccagcgatc
     6961 attcccattg cctttgcgcg cggatccatt ccgacgggaa agagatcgtt gccgggtaca
     7021 ttgttttctg gctgattatc gatgaagccc atctgcacag tctagcggta aaaaaggaat
     7081 tccgggggaa gggaatcgct tttcgtctga tgcagtcgat gaaaaccatt gccggaaaaa
     7141 acgacgtttt attccagacg ctggaagtca gagcctcgaa tgtcgaagca ataaagcttt
     7201 accggaagtg cggttttgta gtaaaaggag tccgaccgct ttattactcg gacacccatg
     7261 aagacgcgct gatcatgtgg gcagatatta atcctgtcag tgaataaaag gattgtgaag
     7321 atggccggaa at
//
LOCUS       DAONQZ010000093         7115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_11208_length_7115_cov_16.865297, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000093 DAONQZ010000000
VERSION     DAONQZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11208_length_7115_cov_16.865297"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..522)
                     /locus_tag="PLR13_10725"
     CDS             complement(10..522)
                     /locus_tag="PLR13_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19855.1"
                     /translation="MGGYGSGKKYWRSAKEKVENCRSIDANSFSRWGYFKPGGRGGIM
                     KWTRNGMETGSCWVYSNIDKLDDSISFSYQYQDKPHPDVKVKLSWYSPGFGGRRYFFR
                     CPVCGKRMRTLHFRYGEIACRVCFNLTYESCVENKRFNGLLKFLKVSLLTRQAQPKKP
                     RGRPRKQMPA"
     gene            complement(548..871)
                     /locus_tag="PLR13_10730"
     CDS             complement(548..871)
                     /locus_tag="PLR13_10730"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HQC19856.1"
                     /translation="MKETRTQSYKSVNLPHGSNSGRPDFRDMPKCQARAKSTGEKCKN
                     VAVKGKRVCYLHGGKSTGAKTERGKMLSKYARLSHGDYSAQVKENRRKLNALNRYLRM
                     LGYLK"
     gene            complement(1362..2195)
                     /locus_tag="PLR13_10735"
     CDS             complement(1362..2195)
                     /locus_tag="PLR13_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19857.1"
                     /translation="MSKYKPQKDQADILDKAYHLIQSVPYRVTARWLFYRLLQESCFK
                     DKDAYKAKFLPLLSKARKSFYKKWTPETLADDTRITVIRGNGWTSEMGFLKAVGRAVC
                     TLDKWQDQDYYVELWFEAKAMKAQFEYYTDHITLRPFGGDCSIAFKWQIAKELEEAYK
                     RYGKPIVILYFGDMDPKGFQIPISAVKDIRQWAKVDFEFIRCGLNQGDEVTYNIPENP
                     DKPGTYQWEALSDEAARELIKNWTEKYISQGLFQSTESLEKDITNQFHHRWSRFIGQA
                     R"
     gene            complement(2297..2500)
                     /locus_tag="PLR13_10740"
     CDS             complement(2297..2500)
                     /locus_tag="PLR13_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19858.1"
                     /translation="MKRSKLNEIPLREAVAGILEGGGGLFVTMSPGQWDALLSTAYDT
                     GATLLEIDDKEQPVKAYQRRIEA"
     gene            complement(2679..3779)
                     /locus_tag="PLR13_10745"
     CDS             complement(2679..3779)
                     /locus_tag="PLR13_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC19859.1"
                     /translation="MFDDFDKQAGVAPEPRQETLKSLIFRKASDWVLEDTPEPNQIIE
                     NFLDVGDKLAIIASSKTRKSFYTLQFCACAAAGKSFCGLNILSPRRVVYIQFEIQENH
                     LHRRLKRISSALQIEPSELENLHILNARGLGIEGVPGIERLKNEVLRFEPELIVCDPL
                     YKLNTGTENDAKDTKTIMAAFDKLSTDTGAAVGYVHHDAKGAPGERQIQDRGAGSNVL
                     GRDYDAAMVLSPHESEIGALVVECALRNYPPIEPFSIQWLYTEGGYYFNREYGLPPAK
                     KTSKSKTTTPLEGYYQTAEAIIGTDEMDIKLFKAQFKEKTKLADSRIRDFMALMCDPK
                     NPRILSSEERGRGKNIKKIRLACHEADLENLN"
     gene            complement(3772..4707)
                     /locus_tag="PLR13_10750"
     CDS             complement(3772..4707)
                     /locus_tag="PLR13_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19860.1"
                     /translation="MSALSKYQDNLSNPVLPGGGCHSWIMSTCNLGVIAGLSPQEIFT
                     DIRRVLHPSRGDREITDAIEKALQSHGDGNHRGISAPTRQVVRKTLIHNGDAVFNKII
                     SAGKYSTEQELTAASPVAIPESPQDQQRLFFDTVFQEDDFVFTGDGKQPGTSKTIRPT
                     CEWGETGTSGPFVIVNPFTGMPAPKKSGDGMTYRGDGCVKHFRHCLVEFDNKTIEDQI
                     KFWSAIKLPVKCLIHTGNKSLHAWIDLSGVPIGSLEQWEKTIKIDLYEKRLKPLGVDM
                     ACSNPARLSRLPGVFRAEKDQWQRLLWLSKEGCHV"
     gene            complement(5121..5381)
                     /locus_tag="PLR13_10755"
     CDS             complement(5121..5381)
                     /locus_tag="PLR13_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19861.1"
                     /translation="MGTEKNDFSELIKKPRQMDFGAIMSKPYLNEREVAAITGRALST
                     LRNERFMRRGIPYAVVGQRSIRYRLTDVIKFMEKTRINFDEV"
     gene            complement(5570..6754)
                     /locus_tag="PLR13_10760"
     CDS             complement(5570..6754)
                     /locus_tag="PLR13_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19862.1"
                     /translation="MNQAEKDKLSKYGKVVWDFANAKTTDDVLISFFYNLQSAFNFSS
                     DFKERALLQYPTKKLTIDTLSSSEYDLLKMLLRRDKILKSCNELLCVFNEFISIGKYD
                     PVNFMFTIDEQFAEFSNPIDGTYSLIKTNSILIPSKNIEAYINNIQLTEIAKEEVKIR
                     VVPGKDIKTVLKKNIKALIAMCHEIEDFKKEATNRFAEVEKIAGFYPKIFKIHDHLKA
                     IQRELKTTLLEIIEAEQAYESERFKRALEIYNSNFKKIYTVTNNKIVEFDTFNERFFL
                     GSLNEYIFNVAINFCLIGYLKNPEYLGKERLTICQKCDCIFSKSRLNSQQHYCPVCSR
                     KNKMTKEERAKYMAGWRKEQKKRQEKDKKRRLDKEISNLLAEGIKPKEAQELAELKIK
                     DK"
     gene            complement(6856..>7115)
                     /locus_tag="PLR13_10765"
     CDS             complement(6856..>7115)
                     /locus_tag="PLR13_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQC19863.1"
                     /translation="EAAGLPADFRPLHGLRHTYASMLASSGKVDLYTLQKLLTHKSPV
                     MTQRYAHLRDEALRNASTLAGQIIDAAMNTNVVEMDKEEQA"
BASE COUNT         1749 a         1643 c         1328 g         2395 t
ORIGIN      
        1 ctacattcat tatgctggca tctgttttct tggccgtccc cgtggcttct tcggttgagc
       61 ctgacgcgtt aataatgaca ccttcagaaa tttcaaaagg ccattaaacc gtttattttc
      121 aacacaactt tcgtaagtca gattaaagca cacccggcaa gcaatttcgc catatctaaa
      181 atgaagagtt ctcatgcgtt tgccgcagac aggacatcta aagaaataac gacggccacc
      241 aaagcccgga gaataccagg acagtttaac ttttacgtct ggatgtggtt tatcttggta
      301 ttggtaagaa aaacttattg aatcatctag cttgtcaata ttactataaa cccagcatga
      361 tcctgtttcc attccattgc gtgtccattt cataataccg cctcttccgc ctggcttgaa
      421 atatccccac cgagaaaagc tgtttgcatc aatagaacgg caattttcaa ccttttcttt
      481 agcgcttctc caatattttt tccctgaacc gtagccgccc ataaatttct tttacctaaa
      541 tcataagcta ttttaaatat cccaacattc tgaggtaacg atttaacgca tttaatttcc
      601 tacggttctc tttcacttgc gctgaataat cgccatgact aagacgggca tatttagaca
      661 acatttttcc ccgttctgtt ttcgcgccgg tgctcttgcc gccgtgaagg taacagacgc
      721 gcttaccttt tacagcaaca tttttacatt tctcacccgt gctttttgcc cgtgcctgac
      781 actttggcat atcgcggaag tctggccttc cgctgttgct tccatgaggt agattgacac
      841 ttttatagga ttgtgtcctt gtctctttca tttttttgct tccaatgtta cgttttgtca
      901 ggttgtcatg gacttcgttc tagctatctc aaaaacatct atgaaaatta tcttttaatg
      961 ttaacatttg ctaacatctc atggggttcg tttcccaact gtgataattt ttcgcttctt
     1021 ccaattcaag gacgagcgtc ttttacccct tttggttcgt ggccctgtcg agagaccgcc
     1081 gtgtaactta cagcgtccat ttctataaag acctttttgt ttacagggcg tacctgccct
     1141 tgtgctagcg ccgcaggtca tgcctcgcag ttcttcagga tatggaggcg attccatcct
     1201 tacggggtag ccctcttgct tttcccattt agcaaaagcc tcatcacatt tctcgtagta
     1261 tcttttatac agttttctta attctggtgt gctcaattca gtcatccaat ttcatgcggt
     1321 gtaatattta accgtcgctt aaatagcgct tcttcatatt tttatcttgc ttgacctata
     1381 aacctactcc agcgatggtg aaattgatta gttatatcct tttcaagcga ttcagtgctt
     1441 tggaataagc cttggctaat atatttttct gtccaatttt ttataagctc ccgcgccgct
     1501 tcgtcgctta atgcttccca ttgatacgtt cccggcttgt caggattttc gggaatatta
     1561 taagtaactt cgtcgccctg atttaaacca caacggataa attcaaaatc aactttcgcc
     1621 cattgccgaa tgtctttgac tgcacttata ggtatttgaa agcctttcgg atccatgtca
     1681 ccaaaatata aaatgacgat aggctttcca tagcgcttat atgcttcctc caattccttg
     1741 gcaatttgcc acttaaaagc gatagagcag tccccaccga aaggtcttag cgtgatgtga
     1801 tctgtataat attcaaactg agctttcata gccttggctt caaaccaaag ctcaacataa
     1861 taatcttgat cttgccattt atccaatgtg caaacggccc ggccaacggc ttttaaaaaa
     1921 cccatttccg atgtccagcc attcccacga ataacagtta tccgagtatc atcggccaat
     1981 gtttccggcg tccatttttt ataaaatgac tttctagcct tggataacaa aggaagaaat
     2041 tttgctttat aggcgtcctt atctttgaag caggattctt gcaacagccg gtaaaacaac
     2101 cagcgtgccg taactcggta tgggacactt tggataagat gataggcttt atcaaggatg
     2161 tctgcctgat ctttttgtgg tttgtatttg ctcatggttt tatacctcaa ttattaatag
     2221 ccattattca ttgctgccgg atttcgcttg acgttgcccc gtggtgcgtc ccgtgccggt
     2281 gttgtgttct tccggctcat gcttctattc gccgttgata ggcttttacc ggctgttctt
     2341 tgtcatctat ttcaagcaag gtcgctcccg tgtcgtatgc cgttgaaagc aatgcatccc
     2401 attggccggg tgacatggtg acaaacagcc cgccgccgcc ttctaatatt ccggccaccg
     2461 cctctcttag gggtatctcg tttaactttg atcgtttcat gcttcctccc atccttcctt
     2521 ttttgtccgc cctctctgcc ccctctgact tttctatttt gcaatgttct taaggtttag
     2581 aaatagaaat tgcatctaag agacttagat gcatttttta aatttctaaa aaccccctta
     2641 gaacatttag aaactttaga aagtttagaa agttcatttc agtttaaatt ctctaaatca
     2701 gcctcatgac aagcaagccg gatcttcttt atattcttcc cgcgcccccg ttcctcactt
     2761 gataaaatcc gtggattttt cgggtcacac attagggcca tgaagtccct tatccgtgaa
     2821 tcagctaatt ttgttttttc tttaaattga gctttgaata actttatatc catttcatcc
     2881 gttccaataa tcgcctctgc cgtctgatag tagccttcta ggggtgtggt agtctttgat
     2941 ttagacgttt tctttgccgg tggaagtcca tattcacgat tgaaataata gccgccttct
     3001 gtgtaaagcc attggattga aaacggctca atagggggat aattacgcaa ggcacactca
     3061 acaaccaaag cgccgatttc gctttcgtgc ggtgacagca ccattgccgc atcataatca
     3121 cgccccagaa cattagaacc cgcgccccgg tcttgaattt gccgttcccc aggtgcgccc
     3181 ttcgcatcgt ggtggacgta acccaccgcc gcgccggtat ctgttgacag tttatcgaaa
     3241 gccgccatga ttgttttagt gtcctttgcg tcattttcgg tgcctgtatt cagcttataa
     3301 agcgggtcac aaacaatcaa ttcgggttca aaacgcagga cttcgttttt taatctctct
     3361 attccaggaa ctccctcaat tcctaatcct ctggcgttta gaatatgcaa gttttcaagt
     3421 tctgacggtt ctatctgcaa agcggagctt attctcttaa gccgccggtg aaggtggttt
     3481 tcctgaattt caaattgaat atagacaacc cgccgggggg ataagatatt caatccgcaa
     3541 aaagattttc ctgccgctgc acacgcgcaa aactgaagcg tataaaatga ctttctggtt
     3601 ttagagctgg cgattattgc taatttgtct ccaacgtcca gaaagttttc aattatctga
     3661 ttcggttctg gtgtgtcttc taatacccag tcacttgctt tgcgaaaaat aagagatttt
     3721 aaagtctcct gtctaggctc cggcgctact cccgcctgtt tgtcaaaatc atcaaacatg
     3781 gcaaccctcc tttgacaacc agaggagacg ctgccattga tctttttctg cccgaaacac
     3841 ccccggtaaa cgtgatagac gcgccggatt gctgcaagcc atatcaaccc ccaaaggctt
     3901 taggcgcttt tcatacaggt caattttgat tgttttttcc cattgttcaa gactgccaat
     3961 aggcacaccg gaaaggtcaa tccaggcatg aagagacttg ttcccggtgt gaattaaaca
     4021 tttgacaggt agcttgattg cagaccaaaa cttgatttga tcttcaatcg ttttgttgtc
     4081 aaattcaacc agacagtgac ggaaatgctt tacgcaacca tcgccgcgat atgtcatccc
     4141 gtcgccgctt ttctttgggg caggcatacc ggtaaacggg ttcacgataa caaacggccc
     4201 agaggttccg gtctctcccc actcacaagt tggccggatt gttttgctgg ttcccggctg
     4261 tttgccgtcg ccggtgaaaa caaagtcatc ctcttgaaaa actgtgtcaa agaaaagccg
     4321 ttgctgatct tgaggacttt ccggtatcgc aaccggactt gctgccgtca attcctgttc
     4381 tgttgaatac ttacctgcgg aaattatttt gttgaaaaca gcgtcgccgt tatgtataag
     4441 tgtttttcta acgacttgcc ttgttggagc tgatataccc cggtgattgc cgtcaccgtg
     4501 gctctgcaag gctttttcga ttgcatcggt gatttcacga tctcctctgg acggatgcaa
     4561 gactcttcta atgtctgtaa aaatttcctg tggcgataat cccgctatga ctcccagatt
     4621 gcaagttgac attatccagg aatgacaacc tccgccaggc aagaccggat tgctcagatt
     4681 atcttgatat tttgataatg cgctcatgcc gcgccccctt attaattgct gtgttattta
     4741 aattatccat caaccctatc catcctatgg ctttctcatt ttcttttgct ggaagtcctg
     4801 tattaaaagg cgctagagca aaagacttga ttcctgccgc cgtgccgccc tcatcttttc
     4861 catgtgagca gttgatgagg ttctggaagt agttttcttc acccgattct cgttataggc
     4921 cataaacggg tgaatcggac actttgacaa ccggcaatcc tgtttcccgt ccgcatagta
     4981 acccaagcaa gaatagcaat gagcattgac agcttgcttc agggttaacc gtccgccctc
     5041 aaggtgcttt ataagttcct ttttgcctct tgcagtcttc ccatgttttt caatttcgga
     5101 tataagtttt gagttcattc ttaaacctca tcaaaattta ttcttgtttt ttccatgaac
     5161 ttaatcacgt ccgttaagcg ataacggatt gatcgttgac caactaccgc atacgggatt
     5221 ccccggcgca tgaatctttc attgcgtaac gtggacaaag ctcgtccggt aattgccgcc
     5281 acctctcttt cattcaggta cggtttgctc atgattgcgc caaagtccat ctgacgcggc
     5341 ttctttatta attcgctgaa gtcatttttt tctgttccca tttttcctgt ttcccctttc
     5401 aaaagtaatt tgttgttacg gaaataaaaa acccgtaagc tgtgtgctgt acgggtaatt
     5461 tattaccgct tgaagtaaat gtcaaaataa gtgagtaaaa ttatatggat gcgtaaacag
     5521 actaattgct aggtttacag ggtgttgcta ggtttacact tgtaaatgat tatttatcct
     5581 tgatttttag ttcggccaat tcttgtgctt cttttggttt aatgccttct gcaagcagat
     5641 ttgaaatttc tttatccaat ctacgttttt tgtctttctc ttgtctcttc ttttgttctt
     5701 ttctccagcc cgccatgtat ttggcccttt cttctttagt cattttattc tttcgtgaac
     5761 ataccggaca ataatgttgt tggctattca accttgattt gctgaaaata caatcacact
     5821 tttggcaaat agtaagtctt tctttcccta aatattcagg attctttaaa tatccaatca
     5881 aacaaaaatt gattgccaca ttgaaaatat attcattcag agaacctaag aaaaagcgtt
     5941 cattaaatgt atcaaactca actatcttgt tattagtaac agtatatatc tttttaaaat
     6001 tagaattgta aatttctagc gctcttttaa atctttcaga ttcatatgcc tgctctgctt
     6061 caattatttc taaaagagtt gtttttaatt ctcgctggat tgcctttaaa tgatcatgaa
     6121 ttttgaatat tttaggataa aatccggcga ttttttcaac ctctgcaaaa cgatttgttg
     6181 cttctttttt aaaatcttct atttcgtgac acattgcaat caatgccttt atattttttt
     6241 tcagtacggt ctttatgtct tttccaggca caacccttat ttttacttct tctttagcta
     6301 tttctgttaa ttgaatattg ttaatatatg cttcaatatt tttagagggt attaaaatag
     6361 aattcgtttt tattagacta taggtaccat ctattggatt actaaattct gcaaattgtt
     6421 catcgattgt gaacataaaa ttgacagggt catatttccc tatggagatg aattcgttaa
     6481 acacacaaag gagttcatta catgatttta gaattttgtc ccgacgtaac aacattttca
     6541 aaagatcata ttcgctgctt gataacgtat ctatcgtcaa tttctttgtt gggtattgta
     6601 ataaggctct ttctttgaag tcgcttgaaa aattgaatgc agattgaagg ttataaaaaa
     6661 aagatattaa aacatcatct gtagttttag cattggcgaa atcccaaaca acttttccgt
     6721 atttggataa tttgtctttt tcggcttgat tcattgcttt ccctctctaa gcatccccta
     6781 tggtttagcc gggaaaacag aaaaggggtt ctttctgctt atcgggtagc tttccctatt
     6841 cccggccatg tattgtcacg cctgttcttc tttgtccatt tcaaccacgt ttgtattcat
     6901 tgccgcgtct atgatctgtc cggccaatgt tgaggcgttc ctcaatgcct cgtctcttag
     6961 gtgtgcatag cgttgagtca taaccggtga tttatgcgtc aatagttttt gcagggtgta
     7021 taagtcaacc ttgccactag aggccaacat ggaagcgtaa gtgtgcctta gtccatgcaa
     7081 aggccgaaag tccgccggta gtccagccgc ttctt
//
LOCUS       DAONQZ010000094         7100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_11241_length_7100_cov_14.063875, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000094 DAONQZ010000000
VERSION     DAONQZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11241_length_7100_cov_14.063875"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLR13_10770"
     CDS             complement(<1..387)
                     /locus_tag="PLR13_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19864.1"
                     /translation="MKSVSRRVKIFLAVGMLAILAACGVKGNPVGPPPGETHSPAVGN
                     LTVTSSADHVALTWALQDEHSRIRSIGIERIEYAAEDAECRNCADKFERIGRLLAGTT
                     GQNGLARKHFEFVDKNVTQGIRYGYRL"
     gene            complement(362..1750)
                     /gene="argH"
                     /locus_tag="PLR13_10775"
     CDS             complement(362..1750)
                     /gene="argH"
                     /locus_tag="PLR13_10775"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQC19865.1"
                     /translation="MSAGKKPWSGRFQKSTARSVEKFTASIHYDHRLYPCDIEGSIAH
                     ATMLAKQKIIAKKDAQKIIAALKAILKDLEQGKLRLDPADEDIHMAIEKELIRRIGEA
                     GGKLHAARSRNDQILLDERLYLRAEIQTIAGRLTSLQRALVQLAKREIKTIMPGYTHM
                     QKAQPVLLSHYLLAFADMFGRDVRRLQDCRERTNVLPLGAAALAGTGLPINRAETARL
                     LNFPGISTNSMDTVADRDFIAEFIFDAALIMMHLSRFCEDLVLWSSEEFGFIEISDAY
                     TTGSSIMPQKKNPDVAELIRGKTGRVYGDLFAILTLLKGLPMTYNRDLQEDKEPLFDA
                     VDTVKSCLSIFCEMINNTRFHSAKMAASASKGFSTATEIAEYLVEKKVPFRRAHDITG
                     KIVAYCLRQKKDLQALTLKEYQAFYRGIQSDILDRIQPESAVRMRGHIGGTAPQTVLQ
                     RIKEFEKRFSKS"
     gene            complement(1768..2982)
                     /locus_tag="PLR13_10780"
     CDS             complement(1768..2982)
                     /locus_tag="PLR13_10780"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQC19866.1"
                     /translation="MAAKKRKVVLAYSGGLDTSVIVRWLIETYKCEVICFAADVGQKE
                     ELTGLKEKAIKTGASKIYIDDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAKLEGADTVSHGATGKGNDQVRFELTYYALNPNIKIIAVWKDDNWTFRSREEM
                     IDYAGKYKIPIPLSKAKPYSADRNLLHISHEGGILEDPWAEPPEDIFVLTRSPENAPD
                     KPTYVEIDFKKGNPVAVNGKKMSPAKLMDYMNTVAGINGIGRVDMVENRFVGMKSRGV
                     YETPGGTVLWSAHRALESITMDREVMLMRDGLTPKYAQLAYNGFWYAPEMKALQTFID
                     ATQENVNGTVRMKLYKGNAIVAGRKSPNSLYSESFATFEKDQVYNQKDAIGFIKLNGL
                     RLIIENILKNKP"
     gene            complement(3035..3943)
                     /gene="argF"
                     /locus_tag="PLR13_10785"
     CDS             complement(3035..3943)
                     /gene="argF"
                     /locus_tag="PLR13_10785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQC19867.1"
                     /translation="MKKDLLSLYDLDTKDFDIIWRKAGKLKKYLKAGKIHDSLKGKTL
                     GMIFDKSSTRTRISFEVGMYQLGGIALFLTSRDTQIGRGESVADSARMMSRYLNGIMI
                     RTFSQESVEELAQWATIPVINGLTDLLHPCQILSDLFTIIEKKGSYRNVKIAYIGDGN
                     NVANTWIEAAARLPIRLSLACPKGYEPDRTIMTKGRRESQEGIVLCHDPFEAAKNADV
                     LYTDVWVSMGQEAEDKKRKKIFKDYQINSNILKAAKKDAIVMHCLPAHRGEEISSDVI
                     DGPHSVVIDQAENRLHVQKAVLELLL"
     gene            complement(3940..5130)
                     /locus_tag="PLR13_10790"
     CDS             complement(3940..5130)
                     /locus_tag="PLR13_10790"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HQC19868.1"
                     /translation="MNEQEVIAQATGNIMNTYRRFPIVLTKGSGARVWDINGKEYLDL
                     VAGIAVCNLGHSHPQVIAAVREQLDKLTHVSNLYYTEPQAGLAQLLVSHSFADKVFFC
                     NSGAEANEAAIKLARKYAHENMGPDKFELITMKDSFHGRTMATITATGQEKFQFGFTP
                     LLDGFTYVPFNDLSALDKAVSSRTCGVLLEPIQGEGGVNIPDDDYLARVREICDRHGI
                     LMILDEVQTGIGRTGKLFAYEYSAIKPDIMTLAKALGNGFPVGAMLATDKVAKAFVPG
                     NHASTFGGNPLAMAAGTAVLKTILQENILEHCRKTGAYFLSQLKKLQQKHAVINAVRG
                     KGLMLAVKLNIDAAGIVGECLKRGLLVNSTGGQVLRFVPPLIITEQDIDQTVAVLDSV
                     LEGK"
     gene            complement(5154..6044)
                     /gene="argB"
                     /locus_tag="PLR13_10795"
     CDS             complement(5154..6044)
                     /gene="argB"
                     /locus_tag="PLR13_10795"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQC19869.1"
                     /translation="MENISNSIERADILLETLPYIRRFYNKTIVIKYGGHAMVDKDLK
                     DRFAQDIVMMKYIGINPVVVHGGGPQIGELLKKVGKESKFIQGMRVTDEETMNIVEMV
                     LVGMVNKEIVGLINRHGGNAVGLSGKDGNLITAEKYYLNEEKAKHTPPEIIDIGLVGK
                     VKSINTQLITSLAHGNFIPIIAPTGLGDNGETYNINADIVAGEIAAALKAEKLLLLTD
                     VPGVLSKKKELIHTMTNDEALNLIEDGTIEGGMFPKIKCCLKALNGGVQKAHIVDGRL
                     KHAVLLEVFTDKGIGTEIVL"
     gene            complement(6060..>7100)
                     /gene="hslU"
                     /locus_tag="PLR13_10800"
     CDS             complement(6060..>7100)
                     /gene="hslU"
                     /locus_tag="PLR13_10800"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQC19870.1"
                     /translation="QESVQAKAAEIAEERVLDLLLPPRLTEKKVDDILNDPNQEQTVL
                     TMEAEQKKNDATREKMRIWLKEGKFDDRMVELEVTEARSGPMIEIFSAAGMEDMGLNL
                     KDMLGNMMPQKKKRRTVKVPEALEILASEETQKLIDMEKVTRTALERVEQSGIIFLDE
                     IDKIVGGDSQHGPDVSREGVQRDLLPIVEGSNVNTRYGMVKTDHILFIAAGAFSGTKP
                     SDLIPELQGRFPIRVELDSLDKEDFIRILTEPNNALVKQYREMMATEGLRLVFKEDAI
                     AAIAEMATVVNENTENIGARRLYTMMEKLLDDISFDAPDLDKKDIVIDAQYVEEKLND
                     IIEDQDLSRYIL"
BASE COUNT         1470 a         2084 c         1677 g         1869 t
ORIGIN      
        1 caaacgatag ccgtagcgta tgccttgcgt gacattttta tcaacaaact caaaatgctt
       61 tcgggccagt ccgttctgtc cggttgttcc ggccaacaaa cgtccgatgc gttcaaattt
      121 atccgcgcaa tttcggcatt cggcatcttc ggctgcgtat tcgatgcgtt ctatgccgat
      181 ggaacggatt cgtgaatgct cgtcctgcaa cgcccacgtc agcgccacat gatccgccga
      241 ggaagtaacg gttaaattgc cgaccgccgg ggaatgcgtc tcgcccgggg gcggaccgac
      301 aggatttcct ttgacgccgc aggccgccag tatcgccagc atccctaccg ccagaaatat
      361 tttaactctt cgagaaacgc ttttcaaatt ctttaatcct ctgtaatact gtttgcggag
      421 cggttccccc gatatggccc cgcatccgga ccgcgctttc cggttgaatc ctgtcgagga
      481 tatccgattg aatcccgcgg taaaaggctt gatattcctt cagcgtcagc gcctgcaaat
      541 cttttttctg cctcagacag taggccacaa ttttaccggt gatgtcgtgc gcccgccgga
      601 aaggcacctt tttttcaacc aggtattccg caatttcggt ggcggtggaa aatcccttgc
      661 tcgccgatgc ggccatcttc gccgaatgaa accgggtgtt gtttatcatc tcgcaaaaaa
      721 tcgaaaggca gcttttcacc gtatccaccg catcgaaaag cggctccttg tcttcctgca
      781 aatcccggtt gtatgtcatg ggcagccctt ttaaaagcgt cagaatcgcg aacagatcgc
      841 cgtatacccg ccctgttttt ccccggatca gctccgcgac atccggattt tttttctgcg
      901 gcatgatgct gctgccggtc gtataggcat ccgatatttc aataaaaccg aactcctcgg
      961 acgaccacag aaccagatct tcgcaaaacc ggcttaaatg catcatgatc agggcggcat
     1021 cgaaaataaa ctccgcgata aaatcccggt ccgccaccgt atccatgctg tttgtgctga
     1081 tcccgggaaa gttcagcagc ctggcggttt ccgcgcggtt aatcggcagc cccgttccgg
     1141 ccagcgccgc cgcgcccagc ggcagaacat tcgtcctttc gcggcaatcc tgaagccgcc
     1201 gcacatcgcg tccgaacata tccgcaaacg ccagaagata atgcgacagc agcaccggct
     1261 gcgccttctg catatgcgta tagccgggca tgatcgtttt gatttcccgt ttggccagtt
     1321 ggaccagggc gcgctgtaac gatgtcagac gtcctgcgat ggtctgaatt tccgcccgaa
     1381 ggtaaagcct ttcatccagc agaatctgat cgttgcggct tctggccgca tgcagtttgc
     1441 ctcccgcctc tccgatgcgc cggatcagtt ccttttcgat ggccatgtgg atgtcttcat
     1501 cggcgggatc cagccggagt tttccctgct cgaggtcttt cagaatcgcc ttcaacgcgg
     1561 cgatgatttt ctgcgcatcc tttttggcga taatcttctg tttcgccagc atcgtcgcat
     1621 gggcaatcga tccctcaatg tcgcagggat agagcctatg atcgtaatga atggacgctg
     1681 taaatttttc gacgctcctg gctgtggatt tctgaaacct tccgctccat ggctttttcc
     1741 cggcagacat tttttctccc cgatccatta aggtttattt ttcaaaatat tttcgataat
     1801 cagccgcaat ccgttgagct tgatgaaacc gatggcatcc ttctggttgt acacctgatc
     1861 cttttcaaag gtggcgaacg attcgctgta cagggaattc ggcgatttgc ggcccgccac
     1921 gatcgcgttg cccttgtata atttcatccg caccgtgccg ttgacgtttt cctgcgtagc
     1981 atcaatgaag gtttgcagcg ccttcatttc cggagcatac cagaacccgt tgtaggccaa
     2041 ctgcgcatat ttcggcgtca ggccgtcgcg catcagcatt acttcacggt ccatcgtgat
     2101 ggactccagc gcgcggtgcg ccgaccagag cacggttccg ccgggggtct cgtaaacgcc
     2161 ccgcgatttc atgccgacaa aacggttctc caccatatcg acacggccga tgccgtttat
     2221 cccggcaacc gtattcatat aatccatcag ttttgccggc gacatctttt tgccgttgac
     2281 ggccaccgga tttcccttct tgaaatcgat ttcaacgtac gtcggtttgt ccggcgcatt
     2341 ttcgggcgag cgggtcaata caaaaatatc ttccggcggt tcggcccacg gatcttccag
     2401 aattccgcct tcatgcgaaa tatgcagaag atttctgtcc gcactgtagg gtttggcctt
     2461 gctcaacgga atgggaatct tgtatttccc ggcataatcg atcatctctt cccgggaccg
     2521 gaaggtccag ttgtcgtcct tccagaccgc gatgattttg atattcggat tgagcgcata
     2581 ataggtcagt tcgaagcgga cctgatcatt gcccttcccg gttgcgccgt gggaaaccgt
     2641 atcggcgccc tccagcttcg caatttcgat ctgccgtttg gcgatcagcg gccgcgccag
     2701 cgatgtgccc agaagatagg tgccttcata aatcgcgttg gcccgaagcg cggggaaaac
     2761 aaaatcgcgc gcgaactcct cccgcagatc gtcaatgtaa attttgctcg ccccggtttt
     2821 gatcgccttt tccttcaggc cggtcaattc ctccttctgt ccgacgtctg cggcaaagca
     2881 gatgacctcg catttatagg tttcaatcaa ccagcggaca ataaccgatg tgtccagtcc
     2941 tccggaatac gccaaaacaa cttttctttt ctttgctgcc aatgtggata tcctcctgac
     3001 cccgatttgt gggatgaata tgttgatcaa atcgttacag caacaattcc agaacggcct
     3061 tttgcacatg cagacggttt tccgcctggt cgatgaccac cgaatggggg ccgtcgatga
     3121 catcggagga aatctcctcg ccccggtgcg ccggcagaca atgcatcacg atggcatctt
     3181 tttttgccgc cttcaaaatg ttgctgttga tctgataatc tttaaatatt tttttccttt
     3241 ttttatcctc cgcttcctgc cccatgctga cccagacatc ggtatagagc acatccgcgt
     3301 ttttcgccgc ttcaaacgga tcgtggcaca gcacaatgcc ttcctgcgac tcgcggcggc
     3361 ccttcgtcat gatcgttcgg tccggctcat agcctttcgg acaggccagc gacagacgga
     3421 tcggcagccg ggcggccgct tcgatccagg tattggccac gttattcccg tcgccgatgt
     3481 aggcgatctt gacgttacga taggacccct tcttctcgat aatggtaaac agatcgctta
     3541 gaatctgaca gggatgcaga agatccgtca gaccgttgat taccggaatc gtcgcccact
     3601 gcgccagttc ctccacgctt tcctgcgaaa atgtccggat cataatgccg ttgagatagc
     3661 gcgacatcat ccgcgccgaa tcggcaacgg attcgccccg gccgatctgg gtatcgcggg
     3721 acgtcagaaa cagagcgatg ccgccgagct gatacatccc gacctcgaaa gaaattctcg
     3781 tccgcgtgga ggacttgtcg aaaatcattc ccagcgtttt tcctttcagc gaatcgtgga
     3841 ttttgccggc tttcagatat tttttcagtt tgccggcttt cctccagatg atatcaaaat
     3901 ctttcgtgtc gagatcataa aggctcaata aatctttttt catttcccct ccaatacgct
     3961 gtcaagcacc gccaccgtct gatcaatatc ctgttcggta atgatcaggg gcgggacaaa
     4021 ccgcaacacc tgaccgcctg tggaattgac cagcaggccc cgtttcaaac attcgccgac
     4081 aataccagcc gcgtcgatat tcagttttac ggccagcata agtcccttgc cccggactgc
     4141 gttgatcacg gcatgcttct gctgcaattt tttcaattgc gatagaaaat atgcgccggt
     4201 tttccggcaa tgttccagta tattttcctg cagaatggtt ttcaatacgg ccgttcccgc
     4261 cgccatggcc agaggattgc cgccgaacgt cgacgcgtga ttgccgggga caaacgcctt
     4321 ggcaaccttg tccgtggcca gcatcgcgcc caccgggaaa ccgttgccca gcgctttggc
     4381 cagcgtcatg atatccggtt taatggcgga atattcgtag gcaaacagct tccccgttct
     4441 gccgatgccg gtctgcactt catcaagaat catcagaatc ccgtgccggt cgcagatttc
     4501 gcgcacccgt gccagataat catcatcggg aatattgaca ccgccctccc cctgaatcgg
     4561 ttccagcagg acaccgcagg tccggctcga aacagcctta tccagcgccg acagatcgtt
     4621 gaacggaacg taggtaaacc cgtccaacag cggcgtgaaa ccgaactgaa atttttcctg
     4681 accggtggcc gtaatcgtgg ccatggtgcg gccgtgaaac gaatccttca tcgtaatcag
     4741 ttcgaattta tccggcccca tgttttcatg ggcgtacttg cgcgccagtt tgatggccgc
     4801 ttcattggcc tccgcgccgc tgttgcagaa aaaaactttg tcggcaaaag aatggctgac
     4861 cagcagctgt gccaggccgg cttgcggctc ggtgtagtac agattggaaa catgcgtcag
     4921 tttatccagc tgctccctga cggcagcgat cacctgcgga tgcgaatgcc cgagattgca
     4981 gacggcaatg ccggccacca gatcgagata ttccttgccg ttgatatccc agacccgggc
     5041 gcccgaccct ttcgtcaata cgatcggaaa ccgcctgtac gtattcatga tatttcctgt
     5101 agcctgcgct atcacttcct gttcattcat ggtcctgtcc tgtttgtaat attttaaaga
     5161 acgatttctg ttccgatgcc cttgtcggtg aacacctcca gaagtacggc atgtttaagc
     5221 cgcccgtcga cgatatgggc tttttggacg ccgccgttga gcgccttgag acagcatttg
     5281 atcttgggaa acatgccgcc ttcgatcgtt ccgtcctcga tcagattcaa cgcctcgtca
     5341 ttggtcatcg tatggatcaa ttcctttttc ttgctcaaaa caccgggcac atcggtcagc
     5401 agcaggagct tttcggcttt cagcgctgcc gcgatttctc cggcgacgat atccgcattg
     5461 atgttgtacg tctcgccgtt atcgccgagt cccgtgggcg cgataatcgg aataaaattg
     5521 ccgtgcgcca gggacgtgat caactgggta ttgatgcttt tgaccttgcc caccaggccg
     5581 atatcgataa tctccggcgg cgtatgtttt gccttttctt cattcagata atacttctcc
     5641 gccgtaatca ggttgccgtc cttgccgctt aatcccacgg cgttcccgcc gtgccggttg
     5701 atcaggccga caatttcctt gttgaccatg ccgaccagaa ccatttccac gatattcatg
     5761 gtctcctcat cggtcacccg cattccctgg ataaatttcg actccttgcc gacttttttc
     5821 agaagctcgc cgatctgcgg gccgccgccg tgcacgacca ccggattgat gccgatatat
     5881 ttcatcatca cgatatcctg ggcgaaccgg tctttcaagt ccttgtccac catggcatga
     5941 ccgccgtatt tgatcacaat cgtcttgttg taaaaccgcc tgatatacgg cagcgtttcc
     6001 agaagaatat ccgccctttc aatcgaatta gaaatatttt ccatttccaa tctccatttt
     6061 tataaaatat acctgctcaa atcctgatct tcgataatgt cgttgagttt ctcttccaca
     6121 tactgcgcgt cgatcacaat gtccttttta tccagatccg gggcatcgaa ggaaatgtca
     6181 tccagcaatt tttccatcat ggtgtagagc cttcgcgcac cgatattttc cgtattttca
     6241 ttaaccactg tcgccatttc cgcgattgcg gcaatggcat cctccttgaa aaccagcctc
     6301 agcccttccg tcgccatcat ttccctgtac tgtttcacca gcgcattatt cggttccgtc
     6361 aggatgcgga tgaaatcctc cttatccagc gaatccagtt ccacgcgaat cgggaaacgt
     6421 ccctgaagtt ccggaatcag gtccgacggc ttcgtgccgg aaaacgcccc tgcggcgatg
     6481 aataaaatat gatccgtttt gaccatgccg taacgcgtgt tgacattgga gccttccaca
     6541 atcggcagca gatcgcgctg cacaccttcc cgtgatacgt ccggaccgtg ctgggaatcc
     6601 ccgccgacaa tcttatcgat ttcatccagg aaaataatgc cggactgctc gacgcgttcc
     6661 agcgccgtcc gggtgacctt ctccatatcg atcaacttct gggtttcttc cgaggccaga
     6721 atttccagcg cctccggaac cttgaccgtc cttcgttttt tcttctgcgg catcatgttc
     6781 cccagcatgt ctttcagatt cagtcccata tcctccatgc cggcggcgga aaagatttcg
     6841 atcatcggtc cgcttctcgc ttcggtcact tccagttcta ccatacggtc gtcgaatttc
     6901 ccttccttga gccagatgcg cattttttcg cgtgtcgcat cgtttttctt ttgttccgcc
     6961 tccatggtca gaacggtctg ttcctggttt ggatcgttaa gaatatcgtc gacctttttt
     7021 tccgtcagcc gcggcggcaa aagcaaatcc agaacccttt cctcggcgat ctccgccgct
     7081 ttcgcctgaa cgctttcctg
//
LOCUS       DAONQZ010000095         7049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_11346_length_7049_cov_17.671004, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000095 DAONQZ010000000
VERSION     DAONQZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11346_length_7049_cov_17.671004"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLR13_10805"
     CDS             complement(<1..698)
                     /locus_tag="PLR13_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQC19871.1"
                     /translation="MFAWLTSPEAWIALATLTALEIVLGIDNIIFISILVSRLPKNKQ
                     NFARNTGLMLAMLTRLALLFSIFWVIKLTAPWFTVFGQEISGRDMILIGGGLFLLAKA
                     THEIHTSLEGAQEETPRLISSSVAAVLIQIALLDIVFSLDSVITAVGLAKDVSIMAIA
                     IICAVGVMLFAAKPISKFVDAHPTIKILALSFLLLIGVTLVAEGFDVHVPKGYIYFAM
                     AFSVGVEMLNLRMR"
     gene            complement(732..1346)
                     /gene="cobO"
                     /locus_tag="PLR13_10810"
     CDS             complement(732..1346)
                     /gene="cobO"
                     /locus_tag="PLR13_10810"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQC19872.1"
                     /translation="MTMVKTEDPAVYNVRSKKSSGLKTSKENAVPKQKGLVIVITGNG
                     KGKTTSAFGQALRAIGQGYKVFVLQFMKGRKYGEFIAAEKYLSNITIKMSGLDSFVMR
                     DHPAAIDIDMARKGLDIARKAIMSGKYDMVILDEINVALDFKLVDLKDVMKLIKNKPA
                     HVDLILTGRYAPKEIIKLADTVSDVQEIKHHYNAGIKDRAGIEY"
     gene            complement(1343..2317)
                     /gene="meaB"
                     /locus_tag="PLR13_10815"
     CDS             complement(1343..2317)
                     /gene="meaB"
                     /locus_tag="PLR13_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQC19873.1"
                     /translation="MDATIKKICAGDVRAASRLIRDIEDKRPDAKVKIKKLYAHTGKS
                     FIIGITGAPGAGKSTLTDALISEFRKLNKTVGVLAVDPTSPFTGGAILGDRIRMQRHA
                     ADDGVFVRSLATRGALGGLSQAAGNAIHVIEAMGKDIIIVETVGIGQQEIDIINHAHS
                     VVVVLVPGMGDEIQTMKAGLMEIADVFVINKANRPGTGQLQTELSSMLNLSPKTADGW
                     KPPIVLVGDAFERATFDENTAELARKIEEHHCFLEQSGQLTERMHRKVMAEIKDALGA
                     CLMEPLMQSLISSGEMGKLSEKIKNRKSDPYTVAEDITEKFFQKQVKK"
     gene            complement(2336..2737)
                     /locus_tag="PLR13_10820"
     CDS             complement(2336..2737)
                     /locus_tag="PLR13_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQC19874.1"
                     /translation="MSERKIRVMVAKPGLDGHDRGARILARCFRDAGFEVVYTGCHQT
                     PEQIAASAIQEDVDLVGLSCLSGAHRALFPRVVELLREKGAGDIAVIGGGIIPEQDVP
                     MLYESGIKAIFTPGTALDSIVEWIKKNIKTH"
     gene            complement(2885..4567)
                     /locus_tag="PLR13_10825"
     CDS             complement(2885..4567)
                     /locus_tag="PLR13_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQC19875.1"
                     /translation="MYFSEEMQEASLKLTQKWEKEVQKTLKNNPEQKKRFSTVSDTEI
                     KRLYTPEDIKDINYTEDIGVPGEFPYLRGNQVTGYRGRYWTFRMFSGMGSAQDTNRRW
                     HMLLREGQTGLSTAFDFPTLMGYDSDSPKALGEVGKCGVAIDTLEDFLTLMEGIPMDK
                     VTTSMTINPPATVLWAMYCAAADIKGIPLSKIGGTIQNDMLKEFIAQKTFMCPPEPSV
                     KLISDTVEFGTKYVPRWNTISISGYHIREAGATAVQELAFTIRDGIEYVDDVIRRKKM
                     NVDDFASRLSFFFNSHIDFFEEICKMRAARRMWAKIMRDRFHAKDPRSLWLRFHTQTA
                     GCSLTAQQPYNNVIRTTVEALAAVLGGTQSLHTNSLDEVLCLPSEHAVEIALRTQQIL
                     AEETGVANTIDPLAGSYFIESLTNEMEEKAWDYIHKIDAMGGMITAIEKGFPQMEISD
                     AAYRFQKQLDAGEKSMVGVNKYATDVTHPLPLVDIDEKVGEEQIKRLKSVRRKRDSKA
                     LKKSLEDVRNACKKGNNVMPCCIDAVKNLATLQEICDVYRDVYGEYRDPGLY"
     gene            complement(4777..4977)
                     /locus_tag="PLR13_10830"
     CDS             complement(4777..4977)
                     /locus_tag="PLR13_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQC19876.1"
                     /translation="MPEGKVKWFNANKGFGFIQQDEGGDVFVHFSAIQVEGFKTLNEG
                     QRVSFDIQQGKKGLEAENVKPI"
     gene            5378..5950
                     /locus_tag="PLR13_10835"
     CDS             5378..5950
                     /locus_tag="PLR13_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQC19877.1"
                     /translation="MKIKASVLIVLLAALWMTVSAGAAEVKPPQVGQGFPDVELSKPV
                     IAGELKYLGLSSSGSFRINQIKADVVIVEIFSMYCPHCQAEAPAVNELYQLIEENAAL
                     KGKIKIIGIGTGNSIYETDIFKKKYSVKFPLVPDGDFKIHKIMGEVRTPYFIVVKLKG
                     KKADVIYSKAGALGDNKTFLKEIIKSAGLK"
     gene            5960..6547
                     /locus_tag="PLR13_10840"
     CDS             5960..6547
                     /locus_tag="PLR13_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQC19878.1"
                     /translation="MKKKIILAALLICFLAPGAFALSDVFKNNVYETGELKPQDSVLK
                     VKVGQPAPDFTLKAVSGKTISLKDYRGKKNVVISFVPAAWTPVCSDQWPGYNIVKDIF
                     DVNDAVLLGISVDNIPTLYSWTNQMGHLWFDVLSDFWPHGAVADQFGVLRSDGISERA
                     ILIIDKKSIIRYIDVHNINKRPPLESIVRELEKLN"
     gene            6715..>7049
                     /locus_tag="PLR13_10845"
     CDS             6715..>7049
                     /locus_tag="PLR13_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC19879.1"
                     /translation="MEEKKRNRKRKEKENRRSTILKAARKLFLERGFKAVTVDSIAEK
                     AEVSKGSIYLCFESKEEIYTQILIADNISLNERIQNFSLIEASASQLLLEFARIYVDF
                     FLSDKELFR"
BASE COUNT         1716 a         1713 c         1740 g         1880 t
ORIGIN      
        1 ttgcgcatgc gcagattgag catttccacg cccacggaaa acgccatggc aaagtaaata
       61 taccctttgg gcacatggac gtcaaatcct tccgccacca gggtcacgcc gatcagaagc
      121 aggaaagata aagccagaat tttgatggtc gggtgggcgt ccacaaattt gctgatcggt
      181 ttggcggcaa agagcatgac gccgaccgca caaatgatgg cgatggccat gatggaaacg
      241 tcctttgcca gaccgaccgc cgtgatgacg gaatcgagag aaaagacaat atccagcagg
      301 gcaatctgaa tcagaaccgc tgccacactg gatgaaataa gtctgggagt ctcctcctga
      361 gcgccctcca ggcttgtgtg aatttcatgc gtggccttgg ccagcagaaa gagtcctccg
      421 ccgatcagaa tcatgtcccg tccggaaatt tcctggccga ataccgtaaa ccagggcgcg
      481 gtcagtttga taacccagaa gatggaaaac agaagcgcca gtcgtgtcaa catggccagc
      541 atcagccctg tgtttcttgc aaaattctgt ttgtttttcg gcagccggct gaccagaata
      601 gaaataaaga tgatattgtc gatgcccaga acaatttcca atgccgtcag cgtcgccaga
      661 gcgatccacg cttccggact ggttagccat gcaaacatat ttgtcctccg ttaattttaa
      721 agatgaggtg tttaatattc tattcccgcg cggtctttga taccggcatt gtagtgatgc
      781 ttgatttcct gaacgtcgct gacggtatcg gcgagcttga tgatttcctt cggtgcgtag
      841 cggcccgtta aaatcagatc cacatgagcg ggcttgtttt ttataagttt catcacgtct
      901 tttaaatcga caagtttaaa atccagcgcc acattgattt cgtcgagaat gaccatgtcg
      961 tatttaccgc tcataatggc cttgcgggcg atatccagtc cttttcgggc catgtcaata
     1021 tcgatggccg cgggatgatc gcgcatgaca aaactgtcca ggccggacat tttgatcgtg
     1081 atgttcgata aatatttttc cgcggcaata aattcgccgt attttctccc cttcataaat
     1141 tgcagaacaa aaaccttata gccctggccg atggcccgca gggcctgtcc gaacgcggag
     1201 gttgttttcc ccttgccgtt gccggtgatc acgatgacca atcccttttg tttcgggacg
     1261 gcattttcct tcgatgtttt taaacccgaa gatttcttgc tgcgcacgtt gtaaacagcg
     1321 gggtcttccg ttttaaccat ggtcattttt tcacctgttt ttgaaaaaat ttttccgtga
     1381 tgtcttccgc cacggtatag ggatcggatt tcctgttctt tattttctcg gacagttttc
     1441 ccatctcgcc gctgctgatc agggattgca tcaggggttc catgaggcag gcgccgagcg
     1501 cgtctttaat ttcggccatg actttgcgat gcattctttc ggtgagttgg ccgctttgtt
     1561 ccaggaaaca atgatgttct tcaattttcc gcgccagttc ggcggtgttt tcgtcaaagg
     1621 ttgcccgttc aaacgcatcg cccaccagga cgatcggcgg cttccagccg tcagcggttt
     1681 tcggggataa attgagcatg gaagacagtt ctgtctgcaa ctgtccggtt ccggggcggt
     1741 tggccttgtt gatgacaaag acatccgcta tctccatcaa tccggccttc atggtttgaa
     1801 tctcgtcgcc catcccggga accagaacga ccaccacgct gtgggcgtga ttgataatat
     1861 caatttcctg ctggccgatg ccgaccgtct cgacgataat gatatccttg cccatggctt
     1921 caatcacatg gatggcgttg ccggcggcct gagagagccc tcccagagcg ccgcgggtgg
     1981 ccagcgaccg gacaaaaacg ccgtcatctg ccgcatggcg ctgcatgcgg atccggtcgc
     2041 ccaggatggc gccgccggtg aaggggctgg tcggatcgac ggccagcacg ccgacggttt
     2101 tgttcagttt tctgaattcg gaaatcagcg cgtcggtgag cgtgctttta cccgcgccgg
     2161 gggcgccggt aattccgata atgaaagatt tgccggtgtg ggcgtagagt tttttgattt
     2221 ttaccttggc atcgggacgc ttgtcttcga tgtctctgat caggcgcgaa gccgcacgaa
     2281 catcgcccgc gcagattttt ttaatggtgg catccataaa gcttcaagag aatttctagt
     2341 gcgttttgat atttttttta atccattcca caatggaatc gagtgctgtt ccgggagtga
     2401 aaatcgcttt gatgcccgat tcataaagca tgggaacgtc ctgttccgga atgatgccgc
     2461 cgccgatgac ggcgatatcc ccggcgcctt tttcacgcag aagttccaca acccggggga
     2521 aaagggcgcg atgtgcgccg gaaagacagc tcaatccgac caggtcgaca tcttcctgga
     2581 tggcggatgc ggcgatttgt tccggggtct gatggcagcc ggtatagacc acttcaaaac
     2641 cggcatcgcg gaaacagcgg gcaagaattc tggcaccgcg gtcgtgtccg tcgaggcccg
     2701 gtttggcaac cattacgcga atttttcttt ccgacatatt actatctcct taaattatct
     2761 ctaataccaa gttgcattca tcaggatacc gcggcggcga ggaggcaccg gaggcgcatt
     2821 actaatacgt cgaggacgcc gacgacgatg ccaacaaagg tagccgtatg agggcggctt
     2881 ggttttaata cagtcccggg tccctatatt ccccgtaaac gtcgcgatag acatcgcata
     2941 tttcctgcag ggttgccaga tttttcaccg cgtcgatgca gcaaggcatg acgttattcc
     3001 cctttttgca ggcgtttctg acgtcttcaa gagatttttt caaggctttg ctgtcgcgct
     3061 tgcggcgcac actcttgagc ctcttgattt gttcttcgcc caccttttca tcaatatcaa
     3121 caagcggaag cgggtgcgtg acgtcggtgg catatttgtt cacccctacc atggatttct
     3181 cgcccgcatc caactgcttt tgaaacctgt aagccgcgtc ggatatctcc atctggggga
     3241 aacctttttc aatggcggta atcataccgc ccattgcatc aatcttgtga atatagtccc
     3301 acgccttttc ttccatttcg ttggtgagag actcgatgaa ataagagccg gccaggggat
     3361 caatggtgtt ggccacgccg gtttcttcgg ccagaatctg ctgcgtgcgc aacgcgattt
     3421 ccacggcatg ctcggaagga agacagagca cttcatccag cgaatttgtg tgcaacgatt
     3481 gtgtgccgcc caagaccgct gccagagctt ccacggtggt ccggataaca ttattgtacg
     3541 gctgttgagc cgtcaacgaa cagccggccg tctgcgtgtg aaagcgcagc cacagggaac
     3601 ggggatcttt ggcatgaaaa cggtcccgca taatcttcgc ccacatgcgg cgggccgcgc
     3661 gcattttgca tatctcttca aaaaaatcga tatgcgaatt aaaaaagaag gacagccgcg
     3721 acgcaaagtc gtccacattc atttttttac ggcgaatcac gtcatccaca tattcgatgc
     3781 cgtcacggat ggtgaacgcc agttcctgga cggccgtggc tcccgcttcg cggatgtgat
     3841 agccgctgat gctgatggtg ttccagcgcg gaacatattt tgtgccgaat tccacggtgt
     3901 cgctgatgag tttgacggaa ggttccgggg ggcacatgaa tgttttctgt gcgataaatt
     3961 ccttaagcat gtcgttttgg atggtgccgc cgattttgga aagcggtatc cccttgatat
     4021 ctgcagccgc gcagtacatg gcccataaaa cggttgccgg agggttgatg gtcatggagg
     4081 tggtgacttt atccatgggg atgccttcca tgagggtcag gaaatcttcc agggtgtcga
     4141 tggcgacgcc gcatttgccc acctcgccca gcgctttggg cgagtcgctg tcatagccca
     4201 tcagtgtcgg aaaatcgaag gccgtactca atccggtttg cccttcgcgc agcagcatat
     4261 gccagcgtcg attggtatcc tgggcgctgc ccattccgga aaacatgcgg aatgtccagt
     4321 aacgtccccg gtagccggta acctgattcc cgcgcaaata aggaaattca ccgggaacgc
     4381 cgatgtcttc ggtataattg atgtctttga tatcttccgg ggtataaagc cttttaattt
     4441 ccgtgtccga cacggtggaa aaacgttttt tttgttcagg attatttttt aacgtctttt
     4501 ggacttcctt ttcccatttc tgggtcagtt ttaaagacgc ctcctgcatt tcttcggaga
     4561 aatacacgat caagtcctcc cgtatggcgc ggaatgatta ggaatattcg taaaaaaact
     4621 taaagccact aactcaaatt ttttgttttt gtcaagacat attgaagcaa ttcacagcaa
     4681 accacaggaa atctttcatt gttccgtgaa tcaaaaaagc acccgcatca atctctacag
     4741 cgggtgcttt tgttggcgaa tcctgtgttt ataagattat atgggtttaa cgttttctgc
     4801 ttccaaccct tttttgcctt gctggatatc gaatgatacc cgctggcctt cattcagcgt
     4861 tttaaagcct tcgacctgaa tcgcgctgaa atgaacgaaa acatctccgc cctcatcctg
     4921 ttggataaaa ccgaaaccct tgttggcgtt gaaccacttt actttccctt ctggcatagt
     4981 tgcacaaact cctttcgtaa attttccaag tcgaattaac actatgacag aattgcaacc
     5041 ttaaaacctt taaagataaa tgagaatctc tccggcagga taaatacaca ctatgcttta
     5101 aagaatatta cagattaacc ttgtacttca aaatgtttaa attcaacttt tttctcagca
     5161 acatattgtt tgctgcagtg gccctattta atcttaacat ccatcaaagt caagctatat
     5221 ttcatgggat tcgccggaga cgggccaggc gacgtcggat gcttaaaccg ccgcactttg
     5281 agggtttaag caagatcgtg cttaatattt ttcccggaat gtgctattaa aaacacaatt
     5341 caagccgaca atggattgtc ggccaaagga gtgaatcatg aaaattaaag cgtccgtcct
     5401 gattgtactg ctggctgcgt tgtggatgac ggtgtcggca ggagcggctg aagtgaaacc
     5461 ccctcaggtg gggcaggggt ttcccgatgt tgaattgtcc aaaccggtga tcgccggtga
     5521 actgaaatat ctcggactgt ccagcagcgg atcttttaga atcaatcaga tcaaggcgga
     5581 tgtagtgatt gtagaaatat tcagcatgta ttgcccgcat tgtcaggcgg aagctcccgc
     5641 cgtcaacgag ctttatcagc tcattgaaga aaatgcggcg cttaaaggaa aaattaaaat
     5701 catcggaatc ggcacgggca actccatcta tgaaaccgat attttcaaga agaaatacag
     5761 cgtgaagttt cctctggttc ccgatggaga tttcaagatc cataaaatca tgggagaggt
     5821 cagaacgccc tatttcattg ttgttaaatt aaaaggaaag aaggcggacg tgatttattc
     5881 caaagcgggc gctttaggag ataacaaaac tttcctgaaa gagattatta aatcagcggg
     5941 attgaaatag gaggcggcca tgaaaaaaaa gatcattttg gcagcgttgt tgatttgttt
     6001 tcttgcaccc ggggctttcg ccttgtccga tgtttttaaa aataatgttt atgaaacagg
     6061 cgaactgaag cctcaggaca gtgtattgaa agtcaaggtt ggccagcccg cgcccgattt
     6121 cacgctgaaa gccgtcagcg gcaaaacgat ttctctgaaa gactaccgcg gaaagaaaaa
     6181 tgttgtgatt tcctttgtgc cggctgcctg gacgccggtg tgttccgatc aatggccggg
     6241 ctacaacatc gtgaaagaca tctttgacgt caatgacgcc gttttactgg gcatttctgt
     6301 tgacaacatt cccacgctgt attcctggac aaaccagatg ggccatttgt ggtttgatgt
     6361 cctgtctgat ttctggccgc acggtgccgt ggcggatcaa ttcggcgtac tgcgctccga
     6421 cggcatttcg gagagagcca ttttgatcat cgataaaaaa agtatcatcc gttacatcga
     6481 tgtccacaac atcaataagc gtccgccgct ggagagcatt gtccgggaac tggaaaaatt
     6541 aaattaatcc atagaattat cattttcagg ataagccccg tttcaacgcg gatgtttttc
     6601 cttgacacat ccccgatatt aatttatgca ccttccaaat gtttatgaac gccggtcatt
     6661 ttttgtttgg ccttgatttt acgcgaatga tttgaaaagg tgatcaggtt cgacgtggaa
     6721 gaaaaaaaaa gaaacaggaa acgaaaagaa aaggaaaata gacgcagtac gatactcaaa
     6781 gccgcccgaa aactcttttt agaaaggggt ttcaaagctg ttacggttga cagtattgct
     6841 gaaaaggcgg aagtcagcaa gggatccatt tatctttgct ttgaaagcaa agaagaaata
     6901 tacacacaga ttttaattgc cgacaacatt tccctcaacg aacgcattca gaatttttcc
     6961 ctgattgaag cctcggcctc ccagcttctg ctggagtttg cccgtattta tgtcgatttc
     7021 tttctcagcg acaaggaact tttcagaat
//
LOCUS       DAONQZ010000096         6976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_11490_length_6976_cov_12.683572, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000096 DAONQZ010000000
VERSION     DAONQZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11490_length_6976_cov_12.683572"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1204..2568
                     /locus_tag="PLR13_10850"
     CDS             1204..2568
                     /locus_tag="PLR13_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQC19880.1"
                     /translation="MLKFSSIVSRVFLLFICFTALPCRADSLDYRDVITDSIGYCASL
                     RVKSEDVFISDAQYRSNFAALYPVVTLGGRAERYENIDNRDNTTINTIDNEVVGGYDS
                     AWRSSVSLSGQYAVSNWYKKIYETGYYKKLKDTSIHECEAEVKKTIRDVTDIFRSVAE
                     SKIKLMYAREILSRLNEIFRMKQAAFTNGQFSHEDVLTAQTEVLNMEKDITGIHKDMR
                     ESLEKLSNYTGKSYSVDVDIISLPLSSSLPTPDETEIIAQTPEYKARLNEIEALRSRK
                     KSVSNNFLPDISVYGRYDFYNSSPDNMHDSLSDVRATDYSVGLHISLPLFDGGVRKWE
                     REKSFHELKKAQEYARLVFNEKNKDIKTLRVGYAELSKSYSHYKKIKEQYEKIKDINR
                     KSKVLGERSDLDVIEFEKDALLAERDLKIIEHTMAFYEKRLALELNFNQFAGEYGGNG
                     TCRH"
     gene            2540..>3480
                     /locus_tag="PLR13_10855"
     CDS             2540..>3480
                     /locus_tag="PLR13_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQC19881.1"
                     /translation="MAETGLVGIEVISKIHTVSIDMQAVKRRYFIETELSREEVLRIL
                     KDHGIRSRLKKLKSVDDLLKYPCPLIIISPENEFHILLGKKEDKIFIFDCNEKKPREM
                     TTDEFLKFWNGEAIAVYPRFTKTEFFLNMKWLFKEFYKHRPVFTAVITASFFIQLFGL
                     VTPLFIQVIIDKVLAHHALTTLQVVAGAFLVVLVFDTIMNLMRNYLLYHTANKVDAGL
                     GAKVYRHLLSLPFRYFETRKVGNIIARVRELENLRQFMTNISLTVLLDTVFSVVFIVI
                     MWVYSGYLTLIVLGIVFLIALISFIATPMIKQRLDEK"
     assembly_gap    3481..3580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3581..4483
                     /locus_tag="PLR13_10860"
     CDS             <3581..4483
                     /locus_tag="PLR13_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC19882.1"
                     /translation="IAFQMFASQLSGPILRLVRMWQDFQQARLSLDRIGDIINTPPEV
                     VGGAITVKELKGEIVAKDVRFRYAPEALLVLDGVNFTIQAGMMVGIVGRSGSGKSTIA
                     KLIQRLYLPVEGTMMVDGVDIRHMDPLFLRYRTGVVLQECYLFSGTIKENIAMAAPNA
                     GMERIIQAARIAGAHDFISEMPMGYDTYVEERGSSLSGGQKQRIAIARALIMNPNILI
                     FDEATSSLDYESEKIIQQNLNLIRQGRTVIFIAHRLSIMRDCDMIIVIDRGKIVETGN
                     HEALMQKDGLYAYLYKQQEENRLR"
     gene            4480..>5810
                     /locus_tag="PLR13_10865"
     CDS             4480..>5810
                     /locus_tag="PLR13_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQC19883.1"
                     /translation="MSKSDYHDFKPILAEIEERPTNPLGTYFLWTIISLMIIVLLTLF
                     LVKIDVVVSARGKIIPIGDVKVVQPLETGVITKIHVKEGDYVRRGDILLEIDPSVDTA
                     DLEGKERNLNYSRLSMGRIDAVLNEKGFSPAGKDLASEIVKTQMTHYQAQRDVYNSTL
                     KEKEKEALENQSALESLRKEIETINHVLSMTLEEEKRHKALVEIGTLADNRYREKVKE
                     RMNLERERDAKEGQARQLETKLSRIDDEIQTFKSNFREKLLSDYSNNIHERNTLEAEV
                     SSLKFKQEKRFIIAPVSGFVHLLPVKTIGGVVTTAQPVVGIVPEGTPLEVNAIVMNKD
                     IGYVREGQHCVVKVDTYDFQKYGTIEGMVEIINPYSMEEKENKETNKAAENESGGYPV
                     RVKMTAETLKTKNGDVYKVKPGMSATVEVNVGKRRVIEFFLFPIIRYLDEGL"
     assembly_gap    5811..5910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5911..6264
                     /locus_tag="PLR13_10870"
     CDS             <5911..6264
                     /locus_tag="PLR13_10870"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQC19884.1"
                     /translation="KGGFNMAKGDKIDENLEQVAPQQRKLRFTEEGIKTHYSGIFNVG
                     LGAEEVILMFGNPSIDPNVIHIESKIAVSLKTAKRITLALSNLIRRYEAINGVIDVSP
                     AMKEKSGKNEGKPVQ"
     gene            6306..>6976
                     /locus_tag="PLR13_10875"
     CDS             6306..>6976
                     /locus_tag="PLR13_10875"
                     /inference="COORDINATES: protein motif:HMM:NF023426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19885.1"
                     /translation="MKILFLHSNFPAQFRHLAVNLAKTDNRIVFGTTRKEGQIPGVYK
                     LIYKPSREPKRETHHYIKPLESAVLHGQAVYRELNKLKKDGFYPEIVYGHSGWGPTMF
                     IKDVFPRSKLLCYFEWFYNADGSDVGFDPCEPLTPDDKLRIRIKNAPILTDLYSCDAG
                     LCPTFWQKNQFPQEYHNKLKVFHDGIDTDYFSPRKDEKLILPSIDLNLSEAKEIVTYV
                     SRGMEP"
BASE COUNT         1973 a         1462 c         1535 g         1806 t
ORIGIN      
        1 aaaatgaatc tattgatgtt ttcgttgtac ctttttttca ttccgccctc cttaattttt
       61 cgcgcaatga aacacgcgac cttccaggca gtccaagggg tcaagtctcc tcttggctca
      121 tggcaatatt gcctccacgt ccgctattct tttccgcaaa cctacatcct ctcttaatgc
      181 ctgttccatc ctttgaataa cactgctcac gctgctgtaa ctgcgcatta cgaaccgatt
      241 cccaatttct gatagcgaag ctccggtatg ttttctcatt aaatatgtcc ataatgggac
      301 ggttctattt tttgaaataa gttccttatt ttttctcatt aattcctcct tcatttttcg
      361 cataaagata ctcgcagtcc tgtagcgaca cagttacgtt ccaaaacaaa tgttttatta
      421 tgattaatta ttcgcggaat aatattagaa atgcttcttg gattaccatg gtattccgaa
      481 tgataaactg aggtaagaaa acgatcagca tagcgaatca cataacaatt ctgttgatca
      541 aaaggaactt tcactctttt tccttagcat gaaccgttta aagcagttca tccttttgat
      601 ttgaacaacc gtgaatcagt gaaacttttc ttgtgaagct ttttgattct ataagattct
      661 ataagacgcg aatttaataa ataaaaatta tcttcttgtc aataaaattc attaaaaatg
      721 atatttgttt tatcaagaat gatgttatct ttagccgaaa tgaaaatgat agaaaaaaaa
      781 gtggcgagaa aaggcttctg aatttaccct atatgatctg tgagtcgcac ttcgcccgat
      841 catcgtggga aatgacaaaa tgaagtgttt cgatttttta catgattgtg taaattagtc
      901 ttgacttttt tacatataga aacgggattt tatttcggca aacaactgtc agtgccctat
      961 tttcagattg ttcgaaactg ccgaaaagcc gaagaatttc ccggcaaccg cgccggaatt
     1021 ccctcaacgc atttcctgat gataaccgga taaatattga tcagggacag aggcctattt
     1081 gtttagcaaa ttttatttgc atccttgatt gcaaattgat atatgcagcg cgattgtcgc
     1141 agtcgaggca atatttttcc atttacagca gcaaaaataa acatagttag acagtgtgaa
     1201 taattgttaa agttcagcag cattgtttca cgcgtttttt tattgttcat ttgctttaca
     1261 gctcttccct gccgggctga ttctctggat taccgcgatg tgatcaccga cagcatcggt
     1321 tattgcgcgt ctctgcgtgt caagagtgag gatgttttta tttccgatgc gcaataccga
     1381 agcaactttg ccgctctcta tccggtcgtc accctcggcg ggcgcgcgga aagatatgaa
     1441 aatatcgata atcgtgacaa cacgacgatt aacacgatag acaatgaggt ggtcggcggt
     1501 tatgatagcg cgtggagatc gtccgtttct ttatcgggac agtatgccgt ttccaactgg
     1561 tacaagaaaa tttatgaaac gggctattat aaaaaactca aggacacgag catccatgaa
     1621 tgtgaagcgg aagtaaaaaa aacgatccgg gatgttacgg atattttccg ctctgtcgca
     1681 gaaagcaaaa tcaagctgat gtacgcccgt gaaattctgt cgcgtcttaa cgaaattttc
     1741 aggatgaaac aggcagcttt caccaacgga caattttctc atgaggacgt tctgacggca
     1801 caaacagaag ttttgaatat ggaaaaggat attaccggca ttcacaaaga catgcgcgag
     1861 agcctcgaga aactgagcaa ctataccggc aaatcttatt ccgtcgatgt ggatatcatt
     1921 tctttgccgc tgtcatcctc cttgccgaca ccggacgaaa ctgagattat tgcacaaacc
     1981 cccgagtata aagcgcggct gaacgaaatc gaggccctca gatccaggaa aaaatcggtg
     2041 agcaataatt ttctgccgga tatttccgtt tacggccgat acgattttta caacagcagc
     2101 cccgacaata tgcatgattc gctcagtgat gtccgtgcga ccgattacag cgtcggactt
     2161 cacatcagcc tgcccttgtt tgacggaggc gtccgtaaat gggagaggga aaaatctttt
     2221 catgaactga agaaggcgca ggaatacgcc cgcctcgttt tcaacgaaaa aaataaggac
     2281 atcaaaacgc tccgggtcgg atacgccgaa ctttcgaaat catactcgca ttataaaaaa
     2341 atcaaggaac aatatgagaa aattaaggac atcaacagaa aatcaaaagt tctcggcgaa
     2401 agaagcgatc ttgacgttat tgaatttgaa aaagacgctc tgctggcgga acgcgatctg
     2461 aaaatcatcg agcacacaat ggctttttat gaaaaacggc tggcgctcga gctgaacttt
     2521 aatcaatttg caggtgaata tggcggaaac gggacttgtc ggcattgaag ttatctccaa
     2581 gatccacacg gtaagcatag acatgcaagc tgttaaacgg cgctacttta tcgaaaccga
     2641 actgagccgg gaggaagttt tgcgcatcct gaaggatcac ggcattcgtt cgcgtctgaa
     2701 gaagctgaaa tcggtcgacg acctgctgaa atatccgtgt cccttgatca tcatctctcc
     2761 tgaaaacgaa ttccatattc tcctggggaa aaaggaagat aagatcttta tcttcgattg
     2821 caatgagaaa aagccccggg aaatgacaac cgatgaattt cttaaattct ggaacggcga
     2881 ggccatcgcg gtgtatcccc gttttacgaa gacggaattt tttctgaaca tgaaatggct
     2941 ctttaaagaa ttctataaac accgtccggt ttttaccgcc gtcattacgg cttctttttt
     3001 cattcagttg ttcggcctgg tcacgccgtt atttatccag gtcattattg acaaggttct
     3061 tgcccatcac gcgttaacta cgctacaagt cgtcgcggga gcttttctgg ttgtgctggt
     3121 tttcgatacc atcatgaacc tgatgcgcaa ttacctcctt tatcataccg ccaacaaggt
     3181 tgacgcagga ctcggggcaa aggtttaccg ccatctgctt tcgctgccgt ttcgttattt
     3241 tgaaacccgg aaagtaggca atatcatcgc ccgtgtccgc gaactggaaa acctgcggca
     3301 gttcatgacc aatatttctt tgaccgtttt gctcgatacg gtgttttcgg tggtgtttat
     3361 cgtcatcatg tgggtttaca gtggatattt gacactcatt gtgctgggca ttgtttttct
     3421 tatcgcgctt atttcattta tagccacacc gatgatcaag caacgattag atgaaaaatt
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcgcttttc agatgttcgc
     3601 atcgcaactg agcggaccga tcctgcgcct ggtgcgtatg tggcaggact ttcaacaggc
     3661 caggctttca ctggacagaa tcggcgatat cattaataca ccgccggaag tagtcggcgg
     3721 cgccattacg gtcaaagaac tgaaaggtga aattgttgcc aaggatgtcc gtttccgcta
     3781 tgcgccggag gcactgttgg tgctggacgg tgtcaatttc actatccaag caggtatgat
     3841 ggtgggtatt gtcggacgaa gcggttccgg caaaagcacg attgccaaac tgattcagcg
     3901 gctttatctg ccggtcgaag gcaccatgat ggtcgacggc gtggatatcc gtcacatgga
     3961 cccgctgttt ctgcgctatc gcaccggcgt tgttttgcag gaatgttatc ttttcagcgg
     4021 caccattaaa gaaaatattg ccatggcggc gcccaacgcc ggcatggaaa gaatcatcca
     4081 ggcggcgcgg attgccggcg ctcatgattt tatttccgaa atgcccatgg ggtacgatac
     4141 ttatgtcgag gaaagaggct cttcattgtc cggcggccaa aagcagcgaa tcgccattgc
     4201 ccgcgcgctg attatgaatc ccaatatttt aattttcgac gaagcaacca gctctctgga
     4261 ttatgaatcg gaaaagatca tccaacagaa cctgaacctg atcaggcaag ggagaaccgt
     4321 tatatttatc gcgcacaggc tttcgatcat gcgcgattgc gatatgatta tagtgattga
     4381 tcggggcaaa attgtggaaa ccggcaatca tgaagctctg atgcaaaaag acggccttta
     4441 cgcttacctc tataaacaac aggaggagaa cagactgaga tgagcaaatc cgattatcac
     4501 gattttaagc ccattctggc ggaaattgaa gaacggccga caaatccgct gggcacctat
     4561 tttctgtgga ctatcatatc gctgatgatt attgttctgc taactctatt tcttgttaaa
     4621 atcgacgttg tcgtttcagc acgcgggaaa atcatcccga taggggatgt caaggttgtt
     4681 cagcctctgg aaacaggtgt catcaccaag atacacgtca aggaaggaga ctatgtcagg
     4741 agaggtgata tcctgctgga aatagatcct tccgtagaca cagctgatct ggaaggcaag
     4801 gaaagaaatc tgaattattc acgtttatcc atgggcagga ttgatgccgt cctcaacgaa
     4861 aaaggttttt ctcccgcagg gaaggacctt gcatcggaaa ttgtaaaaac gcagatgacg
     4921 cactatcagg cgcaaagaga tgtttataat tcaaccctga aagagaaaga aaaggaagct
     4981 ctggagaatc agagcgcgct ggaatccctc aggaaagaaa tcgaaacgat caatcacgtc
     5041 ttgtccatga cgctggaaga ggaaaagagg cacaaagccc tggtggaaat cggcacgctg
     5101 gcggataacc gctatcggga aaaagtcaaa gaaagaatga acctggaacg cgaacgagat
     5161 gccaaagaag gacaggccag gcagttggaa accaaattat cccgaatcga tgatgaaata
     5221 cagactttta aaagcaattt ccgggagaaa ctgctttccg attactccaa taatattcat
     5281 gaacgaaata ctttggaagc ggaagtaagc agtcttaaat tcaaacagga gaaacgattt
     5341 atcatagcgc cggtgtcagg ctttgtccat ctgttgccgg taaaaacaat cggcggcgtc
     5401 gtaacaacgg cccaaccggt cgtaggcatc gtacctgaag gcacgccgct cgaagtcaat
     5461 gccatcgtta tgaataaaga catcggttac gtaagagaag gtcaacattg cgttgttaaa
     5521 gtcgacactt acgattttca aaaatacgga actattgaag gcatggtcga aattatcaat
     5581 ccttatagta tggaagaaaa agaaaataag gaaaccaaca aggccgctga gaatgaatcc
     5641 ggaggctacc ccgtacgcgt caaaatgacg gcggaaaccc tgaaaacaaa aaatggcgat
     5701 gtgtataaag tcaaaccggg gatgtccgcc acagtggaag tgaatgtggg caagcgcaga
     5761 gtgattgagt ttttcctttt cccgataatc cgttatctgg atgaaggtct nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaggaggat tcaacatggc aaagggtgat
     5941 aaaattgatg aaaatttgga acaagttgct ccgcagcaga gaaaacttcg ttttacagag
     6001 gaaggaatta aaactcatta ttccggtata ttcaatgtcg gtctcggagc ggaagaagtt
     6061 attctgatgt tcggcaatcc ttcaatcgat cccaatgtta ttcacataga atcgaaaatt
     6121 gctgtttcgc tgaaaacagc caagcgaatc actctggctt tgagcaatct gattcgccgt
     6181 tatgaagcga tcaacggggt gatagatgtc tctcctgcaa tgaaagaaaa aagcggcaaa
     6241 aatgagggca aacccgttca atgatataga tagttattgc agggagttgg gccgatagac
     6301 gagttatgaa aattcttttc ttgcattcta attttccggc gcagtttcgc catttggcgg
     6361 tcaatttagc caaaactgac aatcgtattg tattcggcac cacgcgcaag gaaggacaaa
     6421 ttcccggtgt ttacaaattg atttacaagc cttcccgtga acctaaacgc gaaacccatc
     6481 attatattaa gccgctggaa agtgccgttt tgcatggaca ggcagtttat cgcgagttaa
     6541 acaaattaaa gaaggacggc ttctaccccg agatagttta tggtcattcc ggttggggac
     6601 cgactatgtt tattaaagat gtcttcccgc ggtctaaact actttgttat tttgaatggt
     6661 tttacaatgc ggatggttcg gatgtcggtt ttgatccgtg cgagccattg acgcctgatg
     6721 acaagcttcg cattcgtatc aaaaatgccc ccattctcac ggatctctac agttgcgatg
     6781 cgggactatg cccaaccttt tggcaaaaga atcaattccc tcaagaatat cacaacaagt
     6841 taaaagtgtt tcatgacggc attgacacag actacttcag cccccggaaa gacgaaaaat
     6901 taatattacc ttccatcgac ctgaatttgt ccgaagcgaa ggaaatagtc acctatgtta
     6961 gccggggaat ggaacc
//
LOCUS       DAONQZ010000097         6737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_11958_length_6737_cov_12.180335, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000097 DAONQZ010000000
VERSION     DAONQZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11958_length_6737_cov_12.180335"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1225)
                     /locus_tag="PLR13_10880"
     CDS             complement(311..1225)
                     /locus_tag="PLR13_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQC19886.1"
                     /translation="MKKNIANVQESVRRRLQNVAKETHRPFAEVLQYYGMERFLFRLC
                     KSAHARQFILKGALMFTAWHLPQRRTTRDIDLLARCDNRTDNIEKIIKDICKTSVPSD
                     GLVFDHQTIHSQRIKEDADYEGVRVRFTGLLERARIPMQIDIAFGDTVYPVPGIVDFP
                     VILDFHKPRLKGYPPESVISEKFEAMVKLGLLNSRMKDFYDIWLMTRQFDFEGMNITA
                     ALSKTFEHRKTPLPVKRPLFAEEIYDEKSDRQTLWTAFLRKNDIKHAPKKLSAAAKLI
                     EKFLLKPLEAIHKNLEFKQEWNAAGKWQ"
     gene            complement(1222..1824)
                     /locus_tag="PLR13_10885"
     CDS             complement(1222..1824)
                     /locus_tag="PLR13_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19887.1"
                     /translation="MRAKKQNLIDYFHKKGGIVRFSAMRKAGFHPDTIAALEKNGQIS
                     KISRGLYQLAGYEPAAHPDLVMVSLLAPRVVICLISALAFHEITSEIPKYVEIAIPKQ
                     SHANIITYPPVRVYRFDHQAWREGVEEHRIDGHTVRVYSPAKTIADCFKFRNKIGVNV
                     ARDAIKTAIAEKRVRPQEIMKYAKICRVSNIIKPILETLI"
     gene            complement(2150..3121)
                     /locus_tag="PLR13_10890"
     CDS             complement(2150..3121)
                     /locus_tag="PLR13_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQC19888.1"
                     /translation="MASKIILERFLWFQNEIKQGRYPNTKTLAEKFEITRKTAQRDIE
                     FMRDRLNAPLMYIPGHRGFTYEDHTYDLPGFWLGEEALISLLLAYRLVSAVPDKSLKT
                     SLRSFLEQLLTKISHSQTMTVKDLNSKISVRNIAYSKTSEKIYHAMLEALLNAGPVKI
                     EYHSPHNSQITTRDILPLHLLHYMGTWHIIAHCALKNELRDFVLSRIQTIAPCAHKIT
                     ARFSSGHVRDYIRKTFGIFHGKQTKSVCLCFSKDVSPWISEQVWHPAQKTFIKKDGRL
                     CLTIPVADFREIKREILKYGSQVEVISPKALRNEIRNEIGKMKKIYR"
     assembly_gap    3314..3413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3414..5014)
                     /locus_tag="PLR13_10895"
     CDS             complement(<3414..5014)
                     /locus_tag="PLR13_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQC19889.1"
                     /translation="MKKIKSMAYMLLIVLLGLSLNTCGSGSDTIDDYIDDLTGRYTIS
                     GTVSGAVAANVTIRLTGAETENTTTAADGTYEFDGLADGTYTVTPVLAGYTFTPASRQ
                     IVIAGADMTANFTAASSSAATPYEQTDLQATWDVQILEAGNINGWGRYNVTVSAAGGV
                     TFNSCQDSTGSTACPAGPIVWTINPNTGVITETNNGADTDNHYAITLNKNFVAGTASG
                     SSQLLIAQKRVSGVAYSNSDLQSKHFVIHSLMVGGENKWLHEVGSTDANRVVTLTSST
                     DPGGTDNNAENTGITISVDANGVVTMNGTMSSYRGFMSYDKRTIVGTFTENGDTYQLM
                     VIQITDGQTGNSVSNVVGTWIDHILAVGGADFWAHQNIAINNTGVMTFSNWVDNDIAI
                     SGPDATETIAIGSTGTATIQGTDFHGQLSYDGQFLIGTRTIDLGSEEAYAFHVSILSA
                     AGSTSTTTYSLSGAVSGATLADVLITLSGAATDSTTTSSSGAFSFTGLANGSYTLIPS
                     KTGYTFTPSSLPVTISGANATGSNFT"
     gene            complement(5399..5608)
                     /locus_tag="PLR13_10900"
     CDS             complement(5399..5608)
                     /locus_tag="PLR13_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19890.1"
                     /translation="MNSLGLIFDIIGVLILFTMDLKMIGLDADAIPMTKDPVKVKKEK
                     IKACIGLGFILFGFTFQFLSNFICK"
     gene            6077..6307
                     /locus_tag="PLR13_10905"
     CDS             6077..6307
                     /locus_tag="PLR13_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19891.1"
                     /translation="MCEIIQAMADDLKYNPPTDQNNGYYNLMQDLTELLRDVKNYEFD
                     DFKNLKYATPKIALRNRLAKISENVINGKYDN"
     gene            complement(6444..6519)
                     /locus_tag="PLR13_10910"
     tRNA            complement(6444..6519)
                     /locus_tag="PLR13_10910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6484..6486),aa:Lys,seq:ctt)
BASE COUNT         1675 a         1485 c         1508 g         1969 t
ORIGIN      
        1 tgataattat tttaccaccc aaccaccccc aatttatgtc ccatcaaaac gttgctcaaa
       61 agatatttta tggatttaaa atgataaaac gttccggaaa tttccgagac agcgtttatt
      121 catttcaaaa catcgggggt ttgatggttt gttattatac ttctttaaaa tattttcaat
      181 ccctcgaaag agggatattg ataattatca ccgtatccgg tgaaacgatt ctggattccc
      241 gccgaaagca tgcgggaatg acttcatttt ttttcagact gatcggggaa caatccaaac
      301 ataatttccc ttattgccat ttgccggcgg cattccattc ttgcttgaat tccagattct
      361 tgtgaatcgc ctccagtggc tttagcagga atttttcaat tagcttggcc gctgccgata
      421 gttttttcgg cgcgtgtttt atatcgttct tccttaaaaa tgccgtccac aatgtctggc
      481 gatcggattt ttcatcataa atttcctctg caaacagtgg acgtttcaca ggcagtggcg
      541 ttttacgatg ttcaaaggtt ttgcttaaag ccgcggtaat attcatgcct tcaaaatcaa
      601 attgccgtgt catcagccag atatcataga aatctttcat acggctgttt aacaagccca
      661 atttcaccat ggcctcgaat ttttcactga tgacactttc cggcggatat cctttgagac
      721 gcggtttgtg aaaatcgagg atgaccggaa agtcaacgat tccagggact ggataaaccg
      781 tatcgccaaa ggcgatgtca atctgcatgg gtatgcgcgc acgttccaat aatcctgtaa
      841 accggacccg cacaccttcg taatcggcat cttctttaat ccgttgactg tgtatcgttt
      901 gatgatcgaa aacaaggccg tcggaaggca cgctcgtttt gcaaatatct tttatgattt
      961 tctcaatgtt gtctgtccgg ttatcgcatc gcgccagtaa atcaatgtcg cgggtcgtgc
     1021 gccgctgagg caggtgccag gcggtaaaca ttagcgctcc tttaagaata aactgacgcg
     1081 catgggcgga tttgcacagc cggaaaagaa atctttccat gccataatac tgcaagactt
     1141 ccgcgaaggg ccggtgcgtt tctttggcaa cgttttgaag ccttctccgg acggattcct
     1201 gcacattggc gatattcttt ttcatatcaa tgtttccaga atcggcttga tgatgttgct
     1261 tacgcgacat attttcgcgt atttcatgat ttcctgcggc ctgacacgtt tttctgcaat
     1321 ggcggttttg atggcgtccc tggctacatt gacgccgatc ttgtttctga atttgaagca
     1381 atccgctatt gtttttgccg gtgagtaaac tctgaccgta tggccgtcta tacgatgttc
     1441 ttcaacgcct tctcgccatg cctgatgatc aaaccggtaa actcgcaccg gcggatacgt
     1501 gatgatattc gcgtgggatt gtttgggtat ggcaatctcc acgtatttgg ggatttcgct
     1561 ggtgatttca tgaaaggcca gcgcggaaat caggcatatc acgacgcgcg gcgccagaag
     1621 agagaccatg accagatcgg gatgcgcggc tggttcgtaa ccggcaagtt gatacaggcc
     1681 ccggcttatt ttggagattt gcccgttttt ctccagcgcg gcaatggtat ccgggtgaaa
     1741 cccggctttt cgcatggccg agaaacgaac aatgccgcct tttttatgga aataatctat
     1801 aagattttgt ttttttgcgc gcatttcctt tccgtatata atgtcaattg ttttaaatat
     1861 gtgtatatga tttatacggt attgtcaata ccgatgttct aatgtcctgc ctgaccgttc
     1921 atcaatattt gtttgtatag gatccggtaa aagcctcgta caccataaaa tcccccctac
     1981 gcggtgctcc ggggatagtt cggcttacgc ttcatacttt gttgcattcg tcttcagctt
     2041 gcctcacgac cccttcatcc ccctttacaa aagggggaga ttaaggcctg gatttaccct
     2101 aacgggcacc atggcccgac aagaccattc gggaatgaca ggcaagatgt tatctgtata
     2161 tttttttcat ttttccaatc tcgtttctta tttcgttgcg cagggctttg ggggatatca
     2221 cttcgacctg cgagccgtat ttgagaattt ctcttttgat ctcccggaag tcggcaacgg
     2281 gaatggtcag gcagagcctg ccgtcttttt tgatgaatgt tttttgcgcc ggatgccaaa
     2341 cctgttcgga aatccagggg gaaacatctt tggaaaaaca caggcagacg gattttgtct
     2401 gttttccatg aaagatgccg aaggttttcc ggatatagtc gcgcacgtgt ccggaagaga
     2461 atcgggcggt aattttatgg gcgcaggggg cgatggtttg tatgcgcgat agaacaaaat
     2521 cccgcagttc attttttaac gcgcaatggg cgataatatg ccaggtgccc atgtagtgca
     2581 gcagatgcag cggcaaaatg tcgcgggtgg tgatttggga attgtgcggc gaatgatatt
     2641 ctattttcac gggcccggca tttaaaagag cttccagcat ggcatgatat attttttccg
     2701 acgttttgga atatgcgatg ttgcggacgg atattttgct gttcaggtct ttgacggtca
     2761 tggtttggga atgcgatatt tttgtcagca gttgttcaag aaacgagcgc agggatgtct
     2821 tgaggctttt gtccggcacg gcggaaacca gacggtatgc cagaagaagt gaaattaatg
     2881 cttcttcacc gagccagaaa cccggcaggt cataggtatg gtcttcgtag gtaaatccgc
     2941 gatggccggg gatatacatc agcggcgcat tcagcctgtc gcgcatgaat tcaatatcgc
     3001 gctgggcggt tttgcgggtg atttcaaatt tttccgcaag ggttttggtg ttgggatacc
     3061 ttccctgttt tatttcgttt tgaaaccaga gaaatcgttc cagaatgatt tttgaagcca
     3121 tgtcccctcc tttgccggat gaatttttac agactgattg aattgtttcg ggtgcaggcg
     3181 gctttcctct ctgtttggtt tatgtttatt tttttaggtg agagaggatt ccggttatca
     3241 atagtgaatt atgccggtaa gcgcaagaat tttttgaatt ggcttttttg aggacgaaaa
     3301 aaaaccgggg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgaaat
     3421 tgctgccggt tgcattggcg ccgctgatgg taacaggtaa actggacggc gtgaaggtgt
     3481 aaccggtttt cgacggtatc agcgtgtagc tgccgttcgc caatcctgta aagctgaatg
     3541 cgccgctgga agacgttgtg gtggagtctg tcgctgctcc cgaaagtgtg atgaggacat
     3601 cggctaatgt cgcaccgctg acggcaccgg acaggctgta ggttgttgtg gatgtcgaac
     3661 ccgcggcaga caggatgctc acatggaatg catacgcttc ttccgaacct aaatcaatgg
     3721 tacgcgttcc aatcaggaac tggccgtcgt aggataactg gccgtggaaa tcagtcccct
     3781 gtatcgttgc cgtgcccgtc gaaccgatgg cgatggtttc cgtcgcatca ggtccggata
     3841 tcgcgatgtc attatccacc cagttgctga acgtcatcac accggtgttg ttgatggcaa
     3901 tgttctgatg cgcccagaag tcagcgccgc cgactgccag gatgtgatcg atccacgtgc
     3961 cgaccacatt gctgacggaa tttcccgtct ggccgtcggt aatctggatg accattaatt
     4021 gataggtgtc gccgttttct gtaaaggtgc ccacgatcgt tctcttgtcg tacgacatga
     4081 agcccctgta ggaagacatg gtgccgttca tcgtcacaac gccgttggca tcaacagaaa
     4141 tggtgattcc tgtattttcc gcgttattat ccgtaccgcc aggatccgtt gagctggtta
     4201 gggtcaccac ccggttggca tcggtgcttc cgacttcatg aagccatttg ttttcgccgc
     4261 cgaccatcag ggagtgaata acaaaatgtt tgctctgcaa atcgctgttg ctgtaggcca
     4321 ccccggatac tcttttttgc gcgataagca attgcgaact gcccgaggct gttccggcaa
     4381 cgaaattttt atttaaggtg atggcataat gattatctgt atcagcgccg ttattggtct
     4441 ctgtaataac gccggtattg ggattaattg tccagacaat cggacccgcc gggcaggcgg
     4501 tgcttcccgt tgaatcctgg caactgttga acgtgacgcc tcccgcggcg ctgacggtta
     4561 cgttgtaacg cccccatccg ttgatgttgc cggcttccag aatctgcaca tcccatgttg
     4621 cctgaagatc agtctgttcg tagggcgttg ccgcactgga agaggctgcc gtaaagtttg
     4681 ctgtcatgtc cgctccggca atgacgattt gccggctggc cggtgtgaag gtgtaaccgg
     4741 ccaaaacagg ggtcacggtg tacgttccgt cggccaaacc gtcgaactcg taagttccat
     4801 cggctgccgt ggttgtattt tccgtctcag cgcccgtcag tcttatcgtc acatttgcag
     4861 caaccgcgcc gctgaccgtt ccggaaatgg tgtaccgtcc cgtaaggtcg tcaatataat
     4921 catcaatggt atcacttccg ctgccgcatg tgttcaatga caggccaagc aaaacgatca
     4981 ataacatgta tgccatcgat tttatttttt tcataagaca ctccttaaat ttatttaggc
     5041 cccgttaaat tttctttctt ttcatagatt atgtttttat taatatattt tcttttccga
     5101 catgtcaata atatctttat tttatagaga tgcgcaggat gaagaaaaaa ggcgtctcgc
     5161 gtgttgtgcg aaacgccttg atatctggtg agccctgtcg gaatcgaacc gacaacctaa
     5221 tgattaagag acacaaatag gcattttctg taaatcatta aatatacatt acattattga
     5281 aatcattaaa acattagctg aaacaattga ggaagattta ggaacaaaac agaatgattt
     5341 ttacaatttt ctttccccaa attttccccg aaaaaatact ttcctaaata ttcctaatct
     5401 acttacaaat aaaattactt aaaaattgaa atgtgaaacc aaacaaaata aaaccaagcc
     5461 cgatacaagc tttaattttt tctttcttaa cttttactgg gtctttcgtc atcggaattg
     5521 cgtctgcatc taatccaatc atttttaaat ccattgtgaa taagattaat actcctatta
     5581 tgtcgaatat aagtcctaaa gagttcatgt cgctactcct tcaaaagaat ttattatcct
     5641 acgcatttat aaatgcacca aataattatt cctataataa tataaaaaga gaattttact
     5701 aacgggtgaa aaaaactgta aggtgaataa gatccatcag caccacactt gggacacaga
     5761 aaatgcatat acccgtctgt gtctttgcca agatttttaa aaatatcatt tggtgggtaa
     5821 tgattaatcg tgtcgtgtgc ctcaactaca ccatgattac cgcatttttt acactttata
     5881 tttcgagtaa tcaatttctg tcccctcctt aaaatattat ccacagctac atctattgac
     5941 ggattttctt atacacccta aaatgaaagt agttcaatat aattaattaa ccttagcaaa
     6001 caaaagtccc gtctaataga attggtcttc cgctaaggtt gccaatattt attttcggga
     6061 tttttgtaaa aaaactatgt gtgaaataat tcaagcgatg gccgatgatt tgaaatacaa
     6121 tccgccaaca gatcaaaata atggctacta caatctaatg caggacctaa ccgaattatt
     6181 aagagatgtt aaaaattatg agttcgatga ttttaaaaat ttaaagtatg ccacaccaaa
     6241 aatcgcactc agaaacagat tagccaagat tagcgaaaac gttataaatg gcaagtatga
     6301 taattaataa aatatttctt tccccagcta ccacacggta gctggctttt ttattggctt
     6361 tgcaaaggac tactgatttt tctttcccca gaaaaatgaa caaaaaaagg cgtctcgcta
     6421 accatgcgaa acgccttgat atctggtgag ccctgtcgga atcgaaccga caacctaatg
     6481 attaagagtc atttgctctg ccagttgagc taagggccct tcaggtctat tggaaaacgt
     6541 caaaggcggc gttgccttcg ccagggcggc tatgcgacgc acatcccgtg cacttcacgc
     6601 ctcgtgtccc ggctgccttg cctctgtctg tttttcaaca gcattgtctt ctaaagaacg
     6661 ttgcactgct ttttattctg gcacgcccgg taggattcga acctacgacc aacagattcg
     6721 aagtctgcga ctctatc
//
LOCUS       DAONQZ010000098         6365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_12825_length_6365_cov_16.003011, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000098 DAONQZ010000000
VERSION     DAONQZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_6365_cov_16.003011"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLR13_10915"
     CDS             complement(<1..270)
                     /locus_tag="PLR13_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19892.1"
                     /translation="MILLRMQCRDFILSGCDPRMGPNSDTSGLNNSTNKFHHAMFLKF
                     ESHYDQQASVIYLESHGAAFESPFASLQGIVRLTLQTSLRSFSAWL"
     gene            400..1581
                     /locus_tag="PLR13_10920"
     CDS             400..1581
                     /locus_tag="PLR13_10920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQC19893.1"
                     /translation="MAYKRMSLNINGISRKFSKLAYSLSELAENFSLIPNNDPKQALR
                     IKRFFISIFVYLLNMPLSYLAYTGGLMEFKELVWGWSITTILMIILYIVFRSGLNKRM
                     KDPSLTFAQIFVASLVVMYGLFFVPEAKGILFAVYVLILLFGTFRLDTRQFLKASAFI
                     LGTYAVDICLLQIYRPEAIHLKLEVLYWFALGVVLASVSFIGGNISALRRDLAVSHKQ
                     LQASVEKIQAMAIHDDLTGFYNRRHLMELLESEHNRSVRTGAVFSLAMIDLDKFKNIN
                     DKFGHQAGDDVLMTFSNVIRNVLRKTDFCGRYGGEEFLIVLTQTDLEEARIFAERIRA
                     CIEDCLVFDLEPDRQLKVTVSIGLAEHLKNEGIDRTIFRADEAMYKAKKNGRNRVEYS
                     E"
     gene            1864..>2638
                     /locus_tag="PLR13_10925"
     CDS             1864..>2638
                     /locus_tag="PLR13_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19894.1"
                     /translation="MKKIVVFLSVCVLVLFMAVRGMAAVWPPADFTESVADQNTAFPQ
                     TMATAVTVFAPLPASCPSHPAACDKLNFLRIRHIHGPDNPSNADRILIAQPGILEGAS
                     AFYNVAANLVTRAYDEKGKFVEFWAIDRRSNCLEDMNGLRLARETGNLHDMVDYYYRN
                     KPYHGQYFQGYFKPLSDDVKWLVEMGMEQTLKDWNEVITRGIPDPTVRQQKVYIGGHS
                     LGGFLTGAFACADFDGDLTTLADAGYNQAAGYFALDSLIT"
     assembly_gap    2639..2738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2739..3695
                     /locus_tag="PLR13_10930"
     CDS             <2739..3695
                     /locus_tag="PLR13_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19895.1"
                     /translation="DAGYNQAAGYFALDSLITSDPLLSVQGGDSATLRYLMSKIPDSA
                     VYLMRKGLFERFIFLSGVITPEIMNLLTGVGYAAMVAPNAETDLISYLPASTSVKNCY
                     RFYHSKNIWDYALVTPSIYKFRYTNQALMALFMDDNAMPINIIQASMGFFAGGTVAEK
                     HFPFGSGGTLAIANNSGTALKKGPLYRWINYDEIEGSAIPNNNDGVPFTSAAKEVTDA
                     KDLARSLGALPMDFVEKYFPMRLAVESMMGSDVIVHPDGISQRPVINIVAGDGAKLGL
                     DQLSPDSPIIPGYNHLDVLTAAAVQNDGEPEKVTTHLLDFIF"
     gene            3853..4494
                     /locus_tag="PLR13_10935"
     CDS             3853..4494
                     /locus_tag="PLR13_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HQC19896.1"
                     /translation="MKYYPVFLDIKDKICVIVGGGEVAFRKAERLLECGAKVSIISPK
                     LVRELAALKEKNLISHIAAEYAGDLIDRAVLIIGATDDEKINALIAQDARARGIPVNI
                     VDDPQKCDFILPAIAQRGDLVMAIGTGGKSPALARHLREELEEQYGREYAILLNILGT
                     LRTKMEKNAGIGRDWFDALMAAGILELIKAKDSQKVQDIVKKITGEEVECEFR"
     gene            4481..5311
                     /gene="ccsA"
                     /locus_tag="PLR13_10940"
     CDS             4481..5311
                     /gene="ccsA"
                     /locus_tag="PLR13_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQC19897.1"
                     /translation="MSFVNLEMLSFNTALVLCGASTIGYLSSLLVKKVKLAKMSTWVL
                     AAAFAFLTVKFFFVMVNSSWFVNPDSKDFLSLCAWAVCAVYLVLQFKTKTRVLGAFIS
                     PLILLFLIAAAGEEAGKSIRPQNLQSGLTAAHLFLTIFGEVLFVIASCAGLMFIMQNR
                     LLKSKKLSGMSRLLPSLNDLDRINHLCLLWGFPILSVGIIAGVIFAEMSWKTGWLTDP
                     KIIWTFAAWMIYGFLLHQRLAIGWKGYRMALISGITFILLIVSYVGVKICCPTLHNFI
                     "
     gene            5314..>6365
                     /gene="hemA"
                     /locus_tag="PLR13_10945"
     CDS             5314..>6365
                     /gene="hemA"
                     /locus_tag="PLR13_10945"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQC19898.1"
                     /translation="MIVLTGLNHKTAPLAVREKIFAGCQEKKDLLPALRSIGGVEEVL
                     YLSTCNRVEILASVAEDGNALKELSGFLARNGGLNQAEAANSFYEYRGEDAVRHIFRV
                     ASSLDSMVMGEAQILGQVKDAYREALAEYATGVVINRLMHCSFRAAKRVRSETGIAVN
                     PVSVSHAAVELAKKIFGTLTGKIILLIGAGEMAELTGTQLIEKGAQSIIVANRSLAQA
                     ELLAEKFHGEAVALDALEKKLIEADIVISSTGAPDFIVTADMLKKIHHRRKNRLLFLI
                     DIAVPRDIDPDASELENVYLYNIDNLQDIVDENINARRKEAIKAEKIIDEELERFINW
                     QKELESVPTIVSLRNKA"
BASE COUNT         1535 a         1469 c         1574 g         1687 t
ORIGIN      
        1 gagccaagct gaaaacgagc gcagcgaagt ttgaagcgta aggcgaacta tcccctgaag
       61 cgaggcgaat ggggattcga aggcagcacc gtgggattcg aggtaaatca ctgaagcttg
      121 ctggtcgtaa tgagattcaa atttcagaaa catagcgtga tgaaatttgt tagtcgaatt
      181 attcaaaccc gaggtatcgg aattaggacc cattcgcgga tcgcaaccgg ataaaatgaa
      241 atcgcggcat tgcatccgca acaaaatcat tgctgattta cggcttcgtc cttttaaaaa
      301 ttatattaag tttatgtggc gttcccgctt gttttgtgct taaggaaacg catagtcacc
      361 tattcttcta atctttttga aaaacagctt tttgcatata tggcttataa aagaatgtcc
      421 ttgaatataa acggtatttc gagaaagttt tccaaactgg cctattctct ttccgaatta
      481 gcggaaaact tttccctcat tcctaataac gatcccaagc aggctttgcg cattaaacgt
      541 tttttcattt caatatttgt ctatcttctg aacatgccgc tttcttatct ggcgtacacc
      601 ggcggcctga tggagtttaa agagctcgta tggggttgga gtatcacgac gattttgatg
      661 attattttgt atatcgtttt tcgcagcggc cttaataaaa gaatgaagga tcccagcctg
      721 acctttgccc aaatattcgt cgccagtctg gttgtcatgt atggcctgtt ttttgttccg
      781 gaagccaagg ggattctttt tgcggtgtat gttcttattc tgttgttcgg aacctttcgc
      841 ctggacacac gtcagttttt aaaagccagt gcgtttattc tggggacata tgcggtggat
      901 atctgtctgc ttcagattta ccggccggag gctattcatc tcaaattgga ggttctttac
      961 tggttcgccc tgggtgttgt tcttgcttcc gtctctttca tcggcggcaa catcagcgct
     1021 ttgcgccgtg atctggctgt gagccacaag cagcttcagg cctctgtgga aaaaatccag
     1081 gccatggcga tacatgatga cctgacgggg ttttataatc gtcgtcatct gatggagctt
     1141 cttgaatccg agcataaccg ctcggtccgt accggcgcgg ttttttctct ggcgatgatt
     1201 gatcttgata aattcaaaaa tattaatgac aagttcggac accaggccgg agatgatgtt
     1261 ttgatgacgt tttccaatgt gatccgcaat gttttgcgaa aaaccgattt ctgcggccgc
     1321 tacgggggag aggaatttct gattgttctg acgcaaacag atctggaaga ggccagaatt
     1381 tttgccgaac gtatccgtgc ctgcattgaa gattgtctcg tttttgatct ggagcccgac
     1441 cgtcaattga aggtaacggt ttccatcggt ttggctgaac atttaaaaaa tgaagggatt
     1501 gaccggacca ttttccgcgc cgatgaagcc atgtacaagg ctaaaaagaa cggacgcaac
     1561 cgcgtggagt attcggaata gcagtaggcc cgccgtcttc aactgcgatc caggataatc
     1621 cttaaattat tttcagaaaa tatctgtgtt cgtccgacta atttttttcg tctgttttcc
     1681 cgcttgacaa acattttcaa tttattttaa gaagaaaaca aattgtacgt tcttcatctc
     1741 acactgtacg tttcttcccc cggacaaatc catataatat aaatttttta cattaaaata
     1801 agtatagtta gctgtgcagg attttcttcg ttcttaattc taaatcaggg gaggaatggg
     1861 tttatgaaaa aaatagttgt atttttatct gtttgtgtat tggttctttt tatggccgta
     1921 agaggcatgg ctgccgtctg gcctccggcg gacttcacgg aaagcgtggc ggatcaaaac
     1981 accgcgtttc cgcaaacgat ggcaacagcg gtgactgttt ttgcaccctt gccggcttcg
     2041 tgtccatccc acccggcagc ctgcgacaaa ctcaattttc tcagaatcag gcatattcac
     2101 ggaccggaca atccctccaa cgcggataga attctgatcg cgcaaccggg tattctggag
     2161 ggcgcatcgg cattctacaa tgtcgcggcc aatctggtga cgcgagcata cgatgagaaa
     2221 ggcaaatttg tggaattttg ggcgattgac agacgatcaa attgtctcga agacatgaac
     2281 ggcctcaggc tggcaaggga aaccggcaat ctgcacgaca tggtggatta ttattaccgg
     2341 aacaaaccgt accacgggca gtatttccag gggtatttta aacccctgag cgacgacgtg
     2401 aaatggcttg tggaaatggg catggaacag accctgaagg actggaatga ggtcatcacg
     2461 cgcggcattc ccgatccgac ggttcgtcag cagaaagtct acatcggcgg tcactctctt
     2521 ggcggattcc tcaccggcgc ttttgcctgc gccgattttg acggcgatct gacaacactt
     2581 gctgacgcgg gatacaatca ggctgccggc tatttcgctc tggattccct gattacatnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cgcgggatac aatcaggctg
     2761 ccggctattt cgctctggat tccctgatta catccgatcc gctgctttcc gtgcagggag
     2821 gggactcggc cactctgcgc tatctgatga gcaagatacc cgacagcgct gtctatttaa
     2881 tgcggaaggg tttgtttgaa cgatttatct ttctttccgg cgtcattact cctgaaatca
     2941 tgaatctact gaccggtgtc ggctatgctg ccatggtcgc accgaatgca gaaacagatt
     3001 taatatcgta tttgccggcc tcaaccagtg tcaaaaactg ctaccggttt taccattcaa
     3061 aaaatatatg ggattacgca ttggtcactc cctccatcta caagttccgg tacaccaatc
     3121 aggctttgat ggccctgttt atggatgaca acgcaatgcc gattaatatc attcaggcaa
     3181 gcatgggttt ttttgcgggg ggaacggttg cggaaaaaca ctttcccttc ggaagcgggg
     3241 gaacgctcgc cattgcaaac aacagcggta ctgccctgaa aaaaggaccg ctctatcgat
     3301 ggatcaatta cgatgaaatc gaagggtctg cgattccgaa caacaatgac ggcgtgccct
     3361 ttacttctgc cgccaaggaa gtaaccgatg caaaggatct ggcgcggtct ttaggcgcgc
     3421 tgccgatgga ctttgtggaa aaatattttc cgatgcggct ggcggtcgag tctatgatgg
     3481 gcagcgatgt gatcgtgcat ccggacggta tttcccaacg gcccgttatt aatattgttg
     3541 cgggtgacgg cgcgaaactc ggtttggacc agttgtcgcc ggattcgccc attatccccg
     3601 gatataatca tctggacgtc ctgacggcgg cagctgtaca aaatgacggc gaaccggaaa
     3661 aggtgaccac acatctactc gatttcatct tttaacagtt tcttcgtaga gaaaacctga
     3721 acaaatcacg gcgtccataa atggacgccg cgatgctttc atcttatacg ttcttgaatt
     3781 ttacccccct tcgtgatagc tattcccaaa atgcttttgg ccgcggcccg tcgggcgaaa
     3841 ggacgaaaac tattgaaata ttaccctgtt tttttggaca tcaaggacaa aatttgcgtc
     3901 atcgtcggcg gcggcgaagt cgccttcaga aaagctgaaa gacttctgga gtgtggcgcg
     3961 aaagtctcca ttatcagtcc gaaacttgtc cgggagcttg ccgcgctcaa agagaagaat
     4021 ctgatttccc acattgccgc cgaatatgcc ggcgacttga ttgaccgggc tgtcctgatt
     4081 atcggggcga ccgatgacga aaagatcaac gccctcatag cgcaggatgc acgggccagg
     4141 ggcatacctg taaatattgt cgacgatccg caaaaatgcg attttattct gcccgccatc
     4201 gcccagagag gcgatctggt catggctatc ggcaccggcg gcaaaagccc ggcactggcg
     4261 cgtcatttac gggaagagct tgaagaacaa tacggcaggg aatacgcaat attgctgaat
     4321 attttaggaa ccctgcgcac caagatggaa aaaaacgccg gaatcggcag agactggttt
     4381 gatgccttga tggcggcagg catcctggaa ttgatcaaag ccaaagacag ccagaaagtg
     4441 caggatatcg tcaagaaaat aaccggtgag gaggtggaat gtgagtttcg ttaatcttga
     4501 aatgttatcg tttaacactg cccttgtcct ctgcggcgca agcaccatcg gttatctgtc
     4561 ttcgcttctt gtcaaaaaag taaaactggc taaaatgtcc acttgggttc ttgccgctgc
     4621 ttttgcattt ctgaccgtta aattcttttt tgtcatggtt aattcttctt ggtttgtaaa
     4681 tcccgactcc aaggattttc tttccctttg tgcctgggcg gtgtgcgcag tttatctggt
     4741 attgcaattt aaaacgaaaa ccagagtgct cggggctttt atttcgccgt tgatcctgct
     4801 ttttttgatt gcggccgccg gagaggaagc gggcaaatct attcgtccgc agaatctgca
     4861 aagcgggctg accgccgccc atttattttt gaccattttc ggggaagtgc tttttgtcat
     4921 cgcttcctgc gccggattga tgttcatcat gcaaaatcgt ctgctgaaga gcaagaagtt
     4981 aagcggtatg agcagacttc tgccttcttt aaacgatctc gatagaatca accatttgtg
     5041 tcttttatgg ggattcccga ttttgagtgt gggcattatt gccggagtga ttttcgcgga
     5101 gatgagttgg aaaaccggct ggctgaccga tccaaaaatc atctggacgt ttgccgcctg
     5161 gatgatttac ggttttctgc tgcatcagcg tctggctatc ggctggaagg gttaccggat
     5221 ggctctcatt tccgggatca cctttattct tcttatcgtg tcttacgttg gcgtgaaaat
     5281 ttgctgtccg acgttgcata actttattta aatatgattg tactgactgg tctgaatcat
     5341 aaaacagccc ccctagctgt ccgggaaaaa attttcgccg gctgtcagga gaaaaaagat
     5401 ttgctcccgg cgctgcgttc catcggcggc gtggaggagg tgctgtatct ttccacctgc
     5461 aatcgtgtgg agattctcgc cagtgttgcg gaagacggca acgctctcaa agaactcagc
     5521 ggattcctgg cacgaaacgg cggtttgaac caggccgaag ctgccaatag cttttatgaa
     5581 tatcgaggcg aagacgcggt gcgccacatt ttccgagtgg cgtccagcct tgattcgatg
     5641 gttatgggcg aagcgcagat tctcgggcag gtgaaggatg cctatcgcga agcgctggcc
     5701 gaatacgcta ccggcgtcgt tataaaccgt ctgatgcact gctctttccg cgcggccaaa
     5761 cgcgtccgct ccgaaaccgg cattgccgtc aatcccgttt cggtgagcca cgccgcggtg
     5821 gagctggcca agaaaatctt tggaacgctt accggcaaaa taatcctgct gatcggcgcc
     5881 ggagagatgg cggagctcac cggcacacaa ctcattgaaa aaggcgctca gtcgattatc
     5941 gtggccaacc ggtcgctggc acaggccgaa ctgttggcgg aaaaattcca cggtgaagcg
     6001 gtggcgctcg atgccttgga gaagaagctc attgaggctg atatcgtcat cagttccacg
     6061 ggcgctcctg attttattgt taccgctgat atgcttaaaa aaattcatca ccggcgtaaa
     6121 aaccgcctgc tgtttctgat tgatattgcc gttccgcgcg acattgatcc ggacgccagt
     6181 gagctggaga atgtttatct gtacaacatc gacaaccttc aggacatcgt tgacgaaaac
     6241 atcaacgccc gccggaaaga ggcgatcaaa gcggaaaaaa ttatagacga agaattggag
     6301 agattcatca actggcagaa ggaactcgaa tccgtgccga caatcgtctc cctgcgcaac
     6361 aaggc
//
LOCUS       DAONQZ010000099         6245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_13137_length_6245_cov_16.975283, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000099 DAONQZ010000000
VERSION     DAONQZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13137_length_6245_cov_16.975283"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..2632
                     /gene="cas10"
                     /locus_tag="PLR13_10950"
     CDS             56..2632
                     /gene="cas10"
                     /locus_tag="PLR13_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HQC19899.1"
                     /translation="MDDTVLKVALAGLLHDIGKFAQEGMSVDSEFINNHRQLYQPYYN
                     GQFTHEHVLYTAAFIDNIEKLLPRELNKANWGLKDPFINLAAGHHKPQTEMQWMIATA
                     DRLSSGYDRANFEQYNQAVAPKDYRQTRLLPLFEGLLRDGGGKYHYPLQELNPRSVFP
                     RQNTEKSKITDAQARDEYKSLFDGFVESLEKILHRDENITLWFEHMESLLLIYTSMIP
                     AARAGRIVPDVSLYDHARSVAAMSAALYLYHRDAGSMNVEAIRDPAGQKFCLIGGDFY
                     GIQNFIFSDSGEAGKHRSKILRGRSFAVSLLCELAADMICRKIGLPSTSILLNAAGKF
                     TIIAPNTPAVKDAVADVQNKINDWLIARTIGENAFGISMLEATPADFESGRFHLLYER
                     LSDQMGENKYKKFDPDKYAGVVAGYLDTFDNELNPRICPYCGKRPSSRAAERLKKDQA
                     DKSWCSICRDHIFLGENIVKKTRIAVTTVDADIRGEDIKLLEPFFGEYQIAFVDGELH
                     KLAREGHLLKYWDISIAPDGHIARDVTARFINGYVPLYGKEDFYDDRILEGKKTESKK
                     EAMIEEMEEGRPKTFAHIACKAMNPEGEGYTGIQALGILKADVDHLGMLMTCGIKEKD
                     FTISRLATLSRQLNFFFVVYLPHLLKTHPDFRDVYTVFAGGDDLFLIGPWNRMLDLSE
                     YLKDRFAEYVCHNPEIHFSAGISLQKANTPLAKMAEDAEEALAVSKNRGRSRITVFGE
                     TVTWDDFSRLHEIVKTIEKWRDEKLINNAMLFRLNRFIQMAAEEKLLLKENEILLEDM
                     EPLKWRSLFRYTAERNIGKNIREEDKRATAKKEFEKMADWLTVHGSSLKIAIWDIIYN
                     NR"
     gene            2645..3061
                     /gene="csm2"
                     /locus_tag="PLR13_10955"
     CDS             2645..3061
                     /gene="csm2"
                     /locus_tag="PLR13_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HQC19900.1"
                     /translation="MNKIAFYKDKTKGIIDPKLFSDIAEQAASDIAKSGQTTMRDGRT
                     RLDHNKRTQIRKFYDEVVRLNGNVKSNPDDWETVLPFINMLIAKAAYANGRNTLVTDD
                     FVALLKDCVSQVQKPADLDVFANFFESFMGFYRKYE"
     gene            3074..3784
                     /gene="csm3"
                     /locus_tag="PLR13_10960"
     CDS             3074..3784
                     /gene="csm3"
                     /locus_tag="PLR13_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HQC19901.1"
                     /translation="MKLTDIKEIKGRIILKSGLHIGAGDTEMHIGGTDNPVLKHPHTQ
                     EPYIPGSSLKGKVRSLLEMESGLMAATKGEPVQVKTLKGLTGEQEAKCRKILKIFGSS
                     GADSGEMTDLGPTRVSFADCPMNATWKKKARDNRWDYFEVKSENSINRIEGTARNPRF
                     TERVVEGAEFDFAVTLKLLDEKESDLFEYLLDGLKLLEMDALGGSGSRGYGRIAFQFE
                     DENLNEKFKTRKPFSNGG"
     gene            3790..4821
                     /locus_tag="PLR13_10965"
     CDS             3790..4821
                     /locus_tag="PLR13_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19902.1"
                     /translation="MKLYEIIIKPKSGFGTPLKGDTIFGHICWQASYNPELLNGGLEK
                     WIAGYGERPFAVFSSAWPKIRKGSAMYYAVKRPDFPPNRLFPPSVKDRRKALEQKKEI
                     LKKKWLLVSENLGISLAAGNFITDEELLTLSFQELTGQTRKAMRKKADRQLKAPFIQP
                     HNTINRATMTTGRGMFAPFAETADFYYPETELAIFVLIDEDATDIDRMSTAIERIGTV
                     GFGRNASTGCGRFSLAKSTEKAWSPKAEADACYTLAPAIPEKGLYKDCWFTPFTRFGR
                     HGDALAVSKNPFKNPVIMTDEGAVFIPADRNVFSKFYLGCAAFNVSKVLSGAVTQGYS
                     FYLPFKLEN"
     gene            4839..>6245
                     /gene="csm5"
                     /locus_tag="PLR13_10970"
     CDS             4839..>6245
                     /gene="csm5"
                     /locus_tag="PLR13_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HQC19903.1"
                     /translation="MFYIKILSPVHIGCDEVYEPAGFIVDEQAGTLVAFDPLDFFRSL
                     DAKTKENYSSICGRGNIESILELYKFMKGKHFTGHAVDVCRGLMAQYQKTLRIPLGDR
                     RKIQQELNNFSISRTAFNPITQKPYLPGSALKGAFRTAYLNHLAKNMTVTVVDRRDKN
                     PANALEKALLEYQSLEKDPFRLMKISDFHPVGPYRTKIIYAVNAKKIPSKFPARGPFQ
                     ILEVIQPGAVFAGSIQVLEPLARDAIKTPLSAEKVFEGARVFYTKEKKREDIELKAAD
                     LPISQTDGDGGICPLRIGRHSGAESLTVEGHRHIKIMKKRGEKPAYSDKATTFWLASE
                     TPDSKTSNRLEPFGWVSLGPMTQTLKDELEARAAEQENIKESYDSKNVSKPDTVEKSV
                     APVKDIEEIWSDATVSYDAGGAGKIVAQGKTQAVLQGTGNRDNLKNAVTEALHKNVFE
                     KKKPVKARVTVRKAGNSWE"
BASE COUNT         1758 a         1461 c         1626 g         1400 t
ORIGIN      
        1 aacagatgat gttttgatat aagcggattc agaaaagaac aggagggaaa tttttatgga
       61 tgatacggtt ttaaaagtgg cgctggccgg actgctgcat gatattggaa aattcgcgca
      121 ggaaggcatg agtgttgatt ctgaatttat caacaatcac cggcagctct accagcctta
      181 ttacaacggg caatttacgc acgagcatgt gctctatacc gccgctttta tcgataatat
      241 cgaaaagctc ttgccgcgtg aattgaacaa ggccaactgg ggcctgaagg atccgtttat
      301 caatctggcg gctggacatc ataagccgca aaccgaaatg cagtggatga ttgcgacggc
      361 tgaccgcttg agcagcggct atgatcgggc taactttgaa caatacaatc aggcggttgc
      421 cccgaaagat tatcgccaaa cgcgactgct tccgttgttt gaaggactgc tgcgtgacgg
      481 cgggggaaaa tatcattacc cattgcagga gttaaaccca cggagcgtct ttccccggca
      541 aaacacagaa aagagcaaga taaccgatgc acaggccagg gatgaatata aaagtctgtt
      601 tgacggcttc gtggaatctc tggaaaagat tctgcacagg gacgaaaaca tcactctctg
      661 gtttgaacac atggaaagtc tgctgctgat ctacacatcc atgattccgg cggcaagggc
      721 gggcaggata gtgcccgacg tttccctgta tgaccatgcc agatcggtcg cggcgatgtc
      781 cgctgctttg tatttatatc accgtgacgc cggcagcatg aatgtcgaag cgatacgcga
      841 tccggccggg cagaaattct gccttatcgg cggggatttc tacggaatac agaatttcat
      901 tttcagtgac agtggcgaag cgggcaaaca ccggtcgaag attcttcgcg ggcggtcttt
      961 tgccgtgtcg ctgttgtgtg aactggccgc tgatatgatt tgccgcaaga ttggtcttcc
     1021 gtcaacctcc attctgctga acgcggcggg gaaatttacc attatcgcgc cgaacacgcc
     1081 ggccgtaaaa gatgccgttg ccgatgttca gaacaaaatc aatgactggc tgattgcgcg
     1141 gaccatcggt gaaaatgcgt tcggcatctc catgctggag gcgacgccgg cagactttga
     1201 atccggccgt ttccacctgt tgtatgagcg cctgtccgac cagatgggcg aaaacaaata
     1261 caaaaagttt gaccccgaca aatatgccgg agttgtagcg ggatatctgg acacatttga
     1321 taatgaactg aatccccgca tttgtcctta ttgcggaaaa aggccgtcca gccgcgcggc
     1381 ggagcggctg aaaaaggatc aggctgataa atcctggtgt tccatctgcc gggaccatat
     1441 cttcctcgga gagaatatcg tcaaaaaaac ccgcattgcg gtgaccactg ttgacgccga
     1501 catccggggc gaagatatta aactgctgga gccgtttttt ggggaatatc agatcgcatt
     1561 tgttgacggg gagcttcata agcttgccag ggaagggcat cttttaaaat actgggacat
     1621 ctcgatagct cccgacggcc acattgccag ggacgtgacc gccaggttca tcaacggcta
     1681 cgtacccctg tacggcaaag aggattttta cgatgaccgc atactcgaag ggaaaaagac
     1741 ggaaagcaaa aaagaagcaa tgattgaaga aatggaggaa ggacgcccga aaaccttcgc
     1801 acatatagcc tgcaaggcga tgaatcctga aggcgaagga tacacgggaa tccaggcctt
     1861 gggtatcttg aaggctgacg tcgatcatct cggaatgctg atgacctgcg gaatcaaaga
     1921 aaaggatttc acaatttcca gactcgctac gctcagccgc cagctgaatt tcttttttgt
     1981 cgtctatctg ccgcatctgt tgaagaccca tccggatttc cgcgatgtct atacggtttt
     2041 tgccggcggc gatgaccttt tcctgattgg cccgtggaac aggatgctcg atttgtctga
     2101 atatctaaag gatcgttttg ccgaatacgt gtgtcataat cctgaaatcc atttctcggc
     2161 gggcatcagt cttcaaaagg cgaacactcc tcttgccaag atggctgagg atgctgaaga
     2221 ggcgctcgcg gtgtcgaaaa acagaggtcg cagccgtatt acggtattcg gggaaaccgt
     2281 aacatgggat gatttttccc gtcttcatga aatcgtcaaa acgatagaaa aatggcggga
     2341 tgaaaagcta attaacaacg ccatgttgtt cagactgaac cgcttcattc agatggctgc
     2401 cgaggagaag ctattgctga aagaaaacga aatattgctg gaagacatgg agccgctcaa
     2461 atggcgttcc ctgttcaggt acaccgctga acgcaacatc ggtaaaaaca tcagggagga
     2521 agataaacgc gctactgcga agaaagaatt tgaaaagatg gcggattggc tgacggttca
     2581 cggcagcagt cttaaaatcg ccatttggga catcatttat aataaccggt aaggaggaag
     2641 gcggatgaat aaaattgctt tttacaaaga caagacaaag gggatcattg atccgaaact
     2701 cttttccgac attgcagagc aggcagcgtc ggacattgcg aaatccggac aaacaacgat
     2761 gagggacgga agaacgcgct tggatcacaa caaacgaaca cagataagaa aattctatga
     2821 cgaggtcgtc cgtctgaacg gaaatgtgaa gagcaatccc gatgactggg aaactgtcct
     2881 gccattcatc aatatgctga ttgccaaagc ggcgtatgca aacggaagaa acactttagt
     2941 tacggacgat tttgttgctt tgttaaagga ttgtgtttcc caggtgcaga agccggcgga
     3001 cctcgacgta tttgccaact tttttgaatc ctttatgggc ttttaccgga aatatgaata
     3061 ggagggagca aagatgaaac ttactgatat aaaagaaatc aaagggagaa ttattcttaa
     3121 aagcgggctt cacatcggcg ccggcgacac ggaaatgcac atcggcggga cggacaatcc
     3181 cgtcctcaaa catccgcaca cgcaggagcc ctacattccc ggttcttcac tcaagggcaa
     3241 ggtgcgttct ctgctggaga tggaaagcgg cctgatggcg gcaaccaaag gcgagcccgt
     3301 gcaggtcaag acactcaagg ggctgaccgg cgagcaggaa gcgaaatgca gaaaaattct
     3361 caagattttc ggttcgagcg gtgcggattc aggcgaaatg accgatcttg gtcccacgcg
     3421 cgtttccttt gccgactgcc cgatgaacgc gacctggaag aaaaaggcga gagataaccg
     3481 ctgggattat ttcgaggtta aatcggaaaa ttccatcaat cgcatcgaag gaacagcccg
     3541 gaatccgcgc ttcacggaac gtgtggtaga gggcgctgaa ttcgattttg ccgtcaccct
     3601 gaaactgctg gatgaaaaag aaagcgacct ttttgaatat cttcttgacg gtctcaaact
     3661 gctggaaatg gacgctctgg gcggcagcgg aagccgcggc tatggccgga ttgcgtttca
     3721 gtttgaagat gaaaatctca atgaaaaatt caaaaccaga aaaccatttt ccaacggagg
     3781 atgaaagccg tgaaactcta tgagatcatc ataaaaccga aaagcggttt cggcacgccg
     3841 ctgaagggcg acaccatttt cgggcatatc tgctggcagg cttcctataa tccggaactg
     3901 ctcaacggcg ggttggaaaa atggatagcc ggttacgggg agaggccctt tgccgtattt
     3961 tcatccgcgt ggccgaagat cagaaaaggc tccgcgatgt attacgccgt gaagcgtcct
     4021 gattttccac ccaaccgcct ttttccgcct tcggtaaagg acagaagaaa agccctggag
     4081 cagaagaaag aaatcctgaa aaagaaatgg cttctggttt ctgaaaatct tgggatttca
     4141 ctcgccgcgg gaaactttat caccgatgaa gaattgctca cgctatcctt ccaggagcta
     4201 acggggcaaa ccagaaaagc catgcgaaaa aaggcagaca ggcagttgaa ggcaccgttt
     4261 atccagccgc acaacaccat caaccgcgcc accatgacaa ccggccgcgg catgtttgcc
     4321 ccttttgcgg agactgctga tttttattat ccggaaacag aactggcgat ttttgttctt
     4381 attgatgaag atgcaacgga tattgaccgt atgagcaccg ccattgaaag aatcggcacg
     4441 gttggcttcg gcagaaacgc ctcgacagga tgcggcagat tttcgttagc gaaatctaca
     4501 gagaaagcct ggtcgccgaa agcagaagct gatgcctgct ataccctcgc gcctgccata
     4561 ccggaaaaag gactttataa ggattgctgg ttcacaccgt ttacccgatt tggccgccat
     4621 ggcgatgcgc tggccgtatc aaaaaacccc tttaaaaatc cggtcatcat gaccgacgag
     4681 ggcgccgttt tcattccggc ggatagaaat gtgttttcca aattctatct cggctgcgcg
     4741 gcgttcaacg tatccaaagt gctttccggg gctgtcacac aagggtattc cttttatctt
     4801 ccattcaaac tggagaattg aaaatggatg aacaaaacat gttttacata aagattcttt
     4861 cgcccgtaca tattggctgt gacgaagtgt atgagccagc cggatttatc gtcgatgagc
     4921 aggctggaac acttgtcgcc tttgacccgc tggacttttt cagaagtctt gatgcgaaaa
     4981 caaaagaaaa ttactcatct atttgcggga gaggcaatat tgaatccata ctggagcttt
     5041 ataagttcat gaaagggaaa cattttaccg gacatgctgt cgacgtttgc agaggcctga
     5101 tggcacagta tcagaaaacc ctaagaatcc ccttgggtga ccgacggaag atacagcagg
     5161 agctaaataa tttttctatc agccgaacgg cattcaaccc gattacacaa aaaccctatc
     5221 ttccgggatc ggctctcaag ggcgcttttc ggaccgctta tttgaaccac ctggcaaaaa
     5281 atatgaccgt taccgtcgtt gatcgccggg ataaaaaccc ggccaatgcc ctggaaaagg
     5341 cgcttttgga atatcagtcg ctggaaaaag atcctttccg cttgatgaaa atatcagatt
     5401 ttcaccccgt cggaccgtac aggacaaaga ttatatatgc ggtaaacgca aaaaaaattc
     5461 cttccaaatt cccggcgcga ggtcctttcc agattctgga agtcattcaa ccgggcgctg
     5521 tttttgccgg aagcattcag gtgttggaac ccttggcgcg tgatgccatt aaaacaccac
     5581 tttccgctga aaaggtcttt gaaggcgcca gagtattcta tacaaaagaa aagaaacggg
     5641 aagatattga actgaaggcc gctgatcttc cgatcagtca gaccgatggg gatggcggca
     5701 tttgtccact gcgcatcggc agacattccg gcgcggaatc cttgaccgtt gaaggccatc
     5761 gccacatcaa gattatgaag aaaagaggtg aaaagcccgc ttattccgat aaagccacaa
     5821 ccttctggct tgcatcggag acgccggaca gcaaaacctc caaccgcctt gagccctttg
     5881 gctgggtgag ccttggcccg atgacgcaaa ccctaaaaga tgaacttgaa gcccgggcgg
     5941 ctgaacaaga gaacataaaa gaatcgtatg attccaagaa tgtatccaaa ccagacaccg
     6001 tggaaaaaag cgttgctccg gtgaaggata ttgaagaaat ctggtcggat gctaccgtat
     6061 cgtacgacgc cggaggcgcg gggaagattg tcgcgcaggg aaaaacgcag gcagtgcttc
     6121 agggtacggg caacagggat aatttgaaaa atgccgtcac ggaagcgctg cataaaaatg
     6181 tctttgagaa gaaaaaaccc gtcaaagccc gcgttaccgt acgcaaggcg gggaatagct
     6241 gggaa
//
LOCUS       DAONQZ010000100         6084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_13576_length_6084_cov_17.616188, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000100 DAONQZ010000000
VERSION     DAONQZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13576_length_6084_cov_17.616188"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLR13_10975"
     CDS             <1..921
                     /locus_tag="PLR13_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19904.1"
                     /translation="YLVDAPDIVLSNRSLPICPVPSIGIGNKPIDGGNYLFTEEEKIE
                     FLKKEPQAEQYFRRWLGADEFLYGYHRYCLWLGDCSPEQLKKMPEAMKRIDAVRNFRL
                     ASKSAPTRKIADTPTRFHVENMPSDEYILISRVSSERRLYLPIGYIEAQNLTSDTALI
                     LIGAKLYHFGILTSIMHNAWMRTVCGRLESRYRYSAGIVYNNFPWPENPTEKQKQAIE
                     TAAQGVLDARAQFPNSTLADLYDPLTMPPVLLKAHQTLDRAVDTAYGKTNFTTEAQRV
                     AFLFELYQKYTSLFAADKPKRRAKVVKIPL"
     gene            975..1433
                     /locus_tag="PLR13_10980"
     CDS             975..1433
                     /locus_tag="PLR13_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQC19905.1"
                     /translation="MRIVMLALCCFICLLTSAHALEMYKCLDNNGNAVITSTPQDGMT
                     DCVLMDSSEDSVPEDNANDNGSETKESVAGSGNANQQADEKLRRQCANLKVYREEERT
                     YCEGVPQSYKSGNEEMKKKAQDRMASSNASASQLCDYYRGMVRELEAKCP"
     gene            complement(1483..2271)
                     /locus_tag="PLR13_10985"
     CDS             complement(1483..2271)
                     /locus_tag="PLR13_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HQC19906.1"
                     /translation="MTDKKNDDIRIAVLENYRKVAVSDQSGCGCSSASCCARPDNAAS
                     LALGYSANDLSVVPEGADMGLGCGNPQAIASLKAGETVLDLGSGGGFDCFLAARSVGD
                     QGLVIGVDMTPEMIAKARRNAEQGGYANVDFRLGELEHLPVANGIVDVIISNCVINLS
                     PEKEKVFREAFRVLKPGGRLAISDIVATAPIPDEIKKDLALYACCIAGASSVRDIENI
                     MTETGFEQIKIILKEESRIFIRTWLPDSHAEDYVVSATIEAIKP"
     gene            complement(2561..3166)
                     /locus_tag="PLR13_10990"
     CDS             complement(2561..3166)
                     /locus_tag="PLR13_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19907.1"
                     /translation="MEALNCGRNIILGGKFGIGRTRLIREIAKLVNERKFIFVDFSQT
                     PGKMSEKIMKALGIPRRIKNQTQQMGYKSMRYRIAHANTIKQHRPVIVFDNMAKITAQ
                     KKILLRHLILEQHFQFIAVVENFLPQKNLFDLKAQLMPATTLNLRHLKKQEVENLLRT
                     YAKKHHVAWTENDIRNPACLSDGYPLSLKEMLKKKIIPEDR"
     gene            complement(3240..4895)
                     /locus_tag="PLR13_10995"
     CDS             complement(3240..4895)
                     /locus_tag="PLR13_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HQC19908.1"
                     /translation="MGNVLPQKSVSDMRRHLKIIADGCSRFRMLSLNRQLEAAQLLLD
                     ENPPIDIAVLGQFKAGKSSFLNSLLGQNVLSVGAIPVTTAVTRLQYGTKAKALVRHFD
                     GNITEIPLADIVKFTSEAENPGNAKNVSIVDIELPSLKKYSGLRLVDTPGLGSVYKYH
                     QSASEEWLPSVGTALLAVSADRPLSEHDLELIRELIAHTPNIILLLTKVDLLSSDQQK
                     EVVAFFQATLNRELHRTLPVYLYSTRDNTDEYKQVIEENIFKTICADRDTEFLRILNH
                     KVRSLSQGCLSYLNIALNSSLQADMNRENLRNRILNEKVNEDIVREELGIISREHQRQ
                     TRALIEAYLEKFRVPLTKKVMRQLVDELPSWKGNLWKLSRKYEVWVSETLSEEMRIIS
                     KNEHRNFLGTMKKAHAAISRSLDAFCNFLGDNIENVLGVKMTEVQWKIDAAEPDHPDI
                     SFTKTFDIHLDLIWFLIPMMFFRKIFERHFIDGIPKEVEINLSRLAYQWEKSVNHAID
                     EMRLQAFNYIHEELATIEALISGTKGQTEDIRGLIDQIEKITI"
     regulatory      complement(4920..4984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5587..>6084)
                     /locus_tag="PLR13_11000"
     CDS             complement(5587..>6084)
                     /locus_tag="PLR13_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQC19909.1"
                     /translation="ELLPRRGAVVAGFSIKFIEDLYDILKVLYLLLSQKGVSKISDEN
                     FNQIAPFFQTMEECANREDFEGYYNAIFDFMEAAIKIVDNPILERLIMNLWPTKKRMM
                     YATLFKRKHDLKSNLDFFLKSVAPYLEKRIDLVENNIVDYLEQEKENAILLAKEFELS
                     NTTKK"
BASE COUNT         1588 a         1372 c         1394 g         1730 t
ORIGIN      
        1 tatctggttg atgcgccgga tatcgtttta agcaatcgaa gtctgccgat ttgtccggtt
       61 ccatctatcg gcataggcaa taaaccgatt gacggcggca actatctgtt tacagaagaa
      121 gagaaaatag aatttctaaa aaaagaacca caggcagagc agtatttccg ccgctggctg
      181 ggagcggatg aatttttgta tggttatcat cgttattgtc tttggctggg tgattgttcg
      241 ccggagcaat taaagaaaat gccggaggca atgaagagaa tagacgctgt aagaaatttt
      301 cgattggcaa gcaaaagcgc tcccacaaga aagattgctg atacaccaac ccggttccat
      361 gtcgagaata tgccaagtga tgaatacatt ttaatttcca gggtttcttc tgaacgtaga
      421 ctttacctac cgattggtta tattgaagcg caaaatctga caagtgatac cgcgcttatt
      481 cttatcggtg caaagcttta ccattttggt atattaacat ctatcatgca caatgcatgg
      541 atgcgcacag tatgcggccg tttggaaagc cgctatcgtt attccgcagg aattgtttac
      601 aacaattttc cctggccgga aaatccgacg gagaaacaaa agcaggccat tgaaaccgcc
      661 gcgcagggcg tgctggatgc ccgcgcgcaa tttccgaact cgacgttggc cgacctctac
      721 gatccgctga ccatgccgcc ggtgctgctc aaagcccatc agacgttaga ccgcgccgtg
      781 gacaccgcct acggcaaaac taattttaca accgaagccc agcgtgtggc gtttctcttt
      841 gaactttatc aaaaatacac cagcctcttt gcagcggaca aacccaaacg ccgagcgaag
      901 gttgtgaaaa taccattgtg atttatacgg cgtgttcagg gaagcctgat gtttagagtg
      961 aaggagaaaa taaaatgaga atcgttatgt tagcgttgtg ttgttttatt tgtttgttga
     1021 cgtccgccca tgcgctggag atgtacaagt gcctggataa taacggcaac gcagttatta
     1081 ccagcacgcc tcaggatggg atgacggatt gtgtgttgat ggattcttca gaagattcag
     1141 tgcctgaaga taacgcaaac gataatggaa gtgaaacaaa agaatcggtc gcgggctcag
     1201 gcaacgccaa tcagcaggcg gacgaaaaat tgcgtcggca atgcgccaat ttgaaagttt
     1261 atcgcgagga agaaaggact tattgcgaag gtgtgccgca gagttataaa agcggcaatg
     1321 aggagatgaa gaaaaaggca caagaccgga tggcgtcttc caacgcgagc gccagccaac
     1381 tctgcgacta ttacagaggt atggtcagag aattggaagc aaaatgcccc tgataatatg
     1441 acctgcgatc aatccgcaaa catttgaacg gattttgtcc cgttacggtt tgatggcctc
     1501 gatggttgcg gaaacaacat agtcttcggc gtggctgtca ggcagccagg tgcgaataaa
     1561 tattctgctt tcttccttga gaattatttt tatttgttca aaacccgttt cggtcatgat
     1621 gttttcaatg tcccgcacgg aagaggctcc cgctatacag caggcataaa gggccagatc
     1681 ttttttgatt tcatcgggta tcggggccgt ggcgacaata tcggaaatcg ctaaccggcc
     1741 gcccggcttt aatacgcgaa acgcttcccg gaatactttc tctttttccg gcgacagatt
     1801 gatgacgcaa tttgaaataa taacatccac gatcccgttc gctaccggca gatgctccag
     1861 ttctcccagc cggaaatcaa cattggcgta accgccttgt tcagcattgc ggcgcgcttt
     1921 tgcaatcatt tccggcgtca tatccacacc gatcacgaga ccttgatcgc cgacagaccg
     1981 tgccgccaga aaacagtcaa agccgccgcc gcttcccaga tccagcaccg tttctccggc
     2041 tttcaacgag gcaatggcct gaggatttcc gcagcccagc cccatgtccg ccccttcggg
     2101 aacaacggat aaatcgttcg ccgaataacc cagcgcaaga gacgcggcgt tatccggtct
     2161 tgcgcaacag gaggcgctgg aacagccgca accggattga tcggacacgg cgacttttcg
     2221 ataattttcc aaaaccgcaa tgcggatgtc gtcgtttttt ttatcggtca tttttagcct
     2281 ctgctttcat cgaagcaata tattgaagat tacagattat tttctatcac aaaattgccg
     2341 tttcaggaat gaagaaatct ggaggagagg atttcctgga caaacccaaa cgctgccggt
     2401 ttactttttt aaatcttaag cagtctgacg gcttcttcca tttcggcggc ttctttcatt
     2461 tttgcccgca tctgccggcg attcctgaag gttttccaaa tgaaccgttg cataacataa
     2521 ttttaagcat gttctcccgg taatgttttt tttaaatctt tcatctgtct tctgggatta
     2581 tctttttttt cagcatctct tttaaactca gtggatagcc atccgataag caagctggat
     2641 tgcgtatatc attttccgtc cacgcaacgt gatgtttttt tgcatacgtg cgcaacagat
     2701 tttcaacctc ctgtttttta agatggcgca ggttcaaagt tgttgcaggc atgagttgcg
     2761 ccttgagatc aaacaaattt ttttgcggca gaaagttttc tacaacggca atgaattgaa
     2821 aatgctgctc cagaatcaga tgacgaagca atattttttt ctgagccgtt attttagcca
     2881 tgttgtcgaa aacaataacc ggtctgtgct gtttgattgt atttgcatga gcaatgcgat
     2941 aacgcatgga tttatagccc atttgttgtg tctgattttt aattcttcgg gggatgccca
     3001 gcgctttcat aattttctcg ctcatttttc ccggcgtttg actgaaatcg acaaagataa
     3061 attttctttc attgaccagc ttcgctattt ctctgattaa acgggttcgt cctatcccga
     3121 actttccgcc cagaataata tttctgccgc aattcagcgc ttccataatt attttcttct
     3181 cgctttggcg tccgacaaat aacataatga ctctgtaaaa cttcgggtga gtatggcgat
     3241 tatatggtta ttttttcgat ctgatcaata aggcctctga tgtcttcagt ttgtcccttt
     3301 gtcccggaaa tcaacgcttc gatcgtggcg agttcttcgt gaatatagtt gaaggcctgc
     3361 aggcgcattt catcgatggc atgattgacg ctcttttccc attgataggc aagccgcgac
     3421 agattgattt ccacttcttt cggtatgccg tcgataaagt gccgttcgaa aatcttgcgg
     3481 aaaaacatca tgggaataag aaaccatatc aggtccaaat gaatatcaaa ggttttggta
     3541 aaagagatat ccggatggtc gggttcagcg gcatcgattt tccattgaac ttccgtcatc
     3601 ttcacaccca gcacgttttc aatgttatct cccagaaaat tgcagaaagc gtccagcgaa
     3661 cgggatatgg cggcgtgcgc ttttttcatg gttcccagaa aattgcgatg ttcgtttttg
     3721 gaaattatcc gcatttcttc ggagagcgtc tccgacaccc agacctcgta ttttcgggat
     3781 agtttccaga gattgccttt ccatgaaggc aattcatcga ccagttgacg cataactttt
     3841 tttgtaagcg gcactctaaa tttttccaga taggcttcaa tcagagcgcg cgtttggcgc
     3901 tggtgttcgc gggaaataat acccagttcc tcccgcacga tatcttcatt gactttttca
     3961 ttcagaatcc ggttgcgcag attttcccgg ttcatatccg cctgcaacga cgaattcagc
     4021 gcaatattga gatagctcag gcagccttga gagagcgacc ggactttatg attcaggatt
     4081 cttaaaaact ccgtgtcgcg gtcggcgcag attgttttaa aaatgttttc ttcaatcacc
     4141 tgtttgtatt cgtccgtgtt gtcccgcgtc gaataaagat aaaccggcag tgtccggtga
     4201 agttcccgat taagtgttgc ctggaaaaag gctaccactt ctttttgctg atccgatgaa
     4261 agcagatcga ctttggtcag caataaaata atattcggcg tgtgcgcgat taattcacga
     4321 atgagttcca aatcgtgttc cgataagggt cggtcggcac ttaccgccag aagcgctgtg
     4381 ccgacggagg gcagccattc ttccgaagcg gactgatgat atttatagac actgcccagt
     4441 cccggcgtgt ccaccaggcg cagaccggaa tatttcttca gggacggcag ttcaatgtcg
     4501 acaatggata catttttcgc gttgcccgga ttttccgctt cggaggtgaa ttttacaatg
     4561 tccgctaacg gaatttcggt gatgtttccg tcaaagtgcc gtacgagggc ttttgctttc
     4621 gtcccatatt ggagacgtgt aacggccgtg gtcaccggaa tggcgccgac ggacaaaacg
     4681 ttttgtccca gaagactatt taagaaagaa cttttcccgg ccttgaattg tcccagcacc
     4741 gcaatgtcga tgggcggatt ttcatcaagc agtaattgcg cggcttccaa ctggcggttc
     4801 agggaaagca tccggaaacg cgagcaccca tccgcaatga ttttcagatg acggcgcatg
     4861 tcggacacgg acttttgagg taagacattt cccataataa aaaaactccc gcagaggtat
     4921 tttctgcagg agttatcagc aataattgcg gttatctcag gtgaactcca tcacccgttt
     4981 gcctgtttgc gtggaccata ttcaaaaaag agtatttagt caatatgttt gtcgagcaat
     5041 ttattaacgg tctcacaatc atactgttat taagcgatta acaaattctc ggatgtcttg
     5101 ccggcgaagg ccgggaacca gtaaaaaata atgccggatg ccggccgtcg ccggcatgac
     5161 gaatgtaaat ttgaatattc gacatttaat aatttgattt gatcatgtgt gagtccgcta
     5221 gaagtcattc ggaatgtttt tccagtgatc gcgtatcaca cgacaaacat cgggcggcag
     5281 aattttattt tgtgtttgtt gttgcaagcc gcgcaggcta tctccagatg aatttgcgta
     5341 cacgtcactc cctgctcgat ccatgtttta tttccatgag ggcgccgcat gaatgcggct
     5401 gcataagtca ttgtgaatgg atgttaattg aggacggaaa acttgagcta cgcgattttg
     5461 aaatatccgc ttcttgatcc atcagtagat tcatcctata tgaaaaataa tttacgaagt
     5521 ttgtgcacgg attataatat gagattatat aaaatgattt tgaataagaa atgaaaaccg
     5581 gaatgtttat ttttttgttg tgttactgag ttcaaactct tttgccaaca ggatggcatt
     5641 ttccttttcc tgttcaagat aatccacgat gttgttttca accagatcaa tccgcttttc
     5701 cagatagggc gcaacgcttt tgagaaagaa atccagattt gatttcaaat cgtgctttcg
     5761 tttgaacaaa gtcgcataca tcatccgctt tttggtcggc cagagattca tgatcaagcg
     5821 ttccagaata ggattgtcga caattttaat cgccgcttcc atgaaatcaa aaatggcatt
     5881 gtagtaacct tcgaagtctt ctcgattggc acattcttcc atggtttgga aaaatggcgc
     5941 tatctgatta aaattttcat ccgaaatttt cgacacgccc ttctgagaaa gaagcaaata
     6001 aagcactttg agaatgtcgt ataaatcttc aatgaatttt atggaaaagc ccgctacgac
     6061 agcgccgcgg cgcggcagta attc
//
LOCUS       DAONQZ010000101         6060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_13646_length_6060_cov_15.300749, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000101 DAONQZ010000000
VERSION     DAONQZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13646_length_6060_cov_15.300749"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..507
                     /locus_tag="PLR13_11005"
     CDS             223..507
                     /locus_tag="PLR13_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19910.1"
                     /translation="MKEGIKEIIGKTIAGVVIAQNKQRAPHQQIFLVFDDETSYEIYG
                     ESFSCASGIYPRGMSGVIRYIKLAPGAELTHVFISKDLNRNKSGRRGKSG"
     gene            741..1307
                     /locus_tag="PLR13_11010"
     CDS             741..1307
                     /locus_tag="PLR13_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC19911.1"
                     /translation="MINTITGNNLVEKAARVAAHAHWTQKRKGDDLPYIIHPVMVAVK
                     LIKHTFPDAVIAAALTHDVLEDTDYPEAKMRAELGDEVLHIVKTVTHDRSLPWEEKKK
                     KYIETVRAGSEGARAVAVSDKIHNLESLLLAYEDQGPALWKRFNRGRDKKLWFEKAML
                     QMLKESWRHPLIDEYERLIEKMTKLKVE"
     gene            1457..4696
                     /locus_tag="PLR13_11015"
     CDS             1457..4696
                     /locus_tag="PLR13_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HQC19912.1"
                     /translation="MTVKRKIIIAVVVVAVVAGAAVLTRAWFYAADKVAQPDSYREYI
                     TKTVSEKMHRSIAYDKSTASLTLRNGLTFNFTHLVISEKDGASDFLRVDEALVRINIL
                     PLLRNRLVFGELVLDRPRVALKRDSSGVLNIADLLIRKEDKKAPRIRKVIIKKGAVTF
                     VDQAADPAGLVTSLENLDGRINALFWMNRYRFHITSSILENNNRASVELSGLFSPVSA
                     GKPVYESKLRASVHLKDADLKHYNAYLKTYTPIEQVSGKLKLDITYTGRFSDFKSKGT
                     VTLRNASLYYPGVFNGYVRPRIAEANFSLKRDKKSLSVDVGRLTVDKFNAKGSFEMDD
                     LDKKDPLLEASAITTAFQLKDVTSYVPWGIIHKGVGDFIQEHIKDGKFRLIEGKLKGR
                     LSQIANINHQEGVDVLSITAEVDKGVFEVVPSAPFFNDIKGILELKRRQFSIKNIRAY
                     FGKSPCTVEGNISDFARPEPNIYTAYMKVQPVRDDVVWLLGEKTFNDMDFRGTSLLTL
                     SGKGPDTGFRIRADWNLTDVAYAFPGVLEKPRSRKNQFTADLVIDDQAVDFQSFKHVM
                     PPVNVTGNMRLRYEGDSPASFSIQSGTFDIREAAPLLPLLQKFDPTGAAQIAVTGQGN
                     LNEPSSLQWKGNIALKNISLDVSDKFSRMQGLTGSVSFKNRSMQTSLFRTQIGRSKIQ
                     GNFWIEDFSDSRVFCRVNSEALKASDLGLKSAKGDVTLRDVKGQLSFEDELIHVERLQ
                     FGAGTSTFILSGDITEFDLPKVTLELSSMYVHSEDLSRLMALSYPSKDQKAKSTVDLN
                     AVFLLDGGTFGDAPFQKLYAVMKLKNDILDVDELEVGIFDGKFKAKGRVVLPAAGPNR
                     YDANITVDKISLDKLQQYLELGDRTVTGDLSLSANISMSGANAGELKQSAMGTVKVRA
                     EKGVLKKFAVLSKIFSLLNIVQLAKLKLPDMATGGMPYSEITFHATFKGGVISSEDFF
                     IDSNAMQISGTGSIDFVRKKLDFVAGVHPLQSLDLIASKIPIAGWIITDERGKLITAH
                     FKVDGTWDDPKVSTITAKSIGKGTLDIFRRIFQLPEKLVTDTGEVILGH"
     gene            complement(4734..5621)
                     /locus_tag="PLR13_11020"
     CDS             complement(4734..5621)
                     /locus_tag="PLR13_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC19913.1"
                     /translation="MKLEKDLYAIDKTLLVQAGETISRKTLQRVASLSGKITYVPIKD
                     TWLMKDLVETFKDERYTNILYPPDINRKIVRSIARIRMPEKILSELAYLKTRSPYTYQ
                     HIMVIAILAGKVSFDHSLKNKYMHDLAVLLSLFHDLGKSRIPLSILDKPTPITIKERR
                     ILTSHPLIGYVLLHYYFGKDHKKYSFSSFQHHERLDGSGYPLGIKTLNKYSQLIGVVD
                     ALDALISERPYRRKPFSLRAAIDLLLDEAERGRFRKSLVRTLIIYSRRENPGAKIVIA
                     QRGRDQAPLDNAYGKTARS"
     gene            5819..>6060
                     /locus_tag="PLR13_11025"
     CDS             5819..>6060
                     /locus_tag="PLR13_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQC19914.1"
                     /translation="MNEMKKDREHWASRVGFILAAAGSAVGLGNIWRFPYMTGENGGA
                     AFIVIYLLAVFLLGYPLMVNETVLGRASQRDPVGAF"
BASE COUNT         1521 a         1294 c         1721 g         1524 t
ORIGIN      
        1 gccggggcat gacagaaaaa agataggagc atgccggggg gcgcgatcta caaaaaaact
       61 gatgcgcccc gccaatattt tcttgtggga cgtaaaatgt cccatcacgt cgtttatgat
      121 catccaaaca gtaaaatatt gttttaagct tttgtgccgg tagtgcgtgt tggcaacagg
      181 gatcattaat gaatggctgt ttggtgatgg aggacaagtg caatgaagga agggattaaa
      241 gaaatcatcg gtaaaacaat agccggtgtc gtgattgctc aaaataagca acgggcgccg
      301 catcagcaga tttttctcgt ttttgacgat gagacgtcat atgaaattta tggcgaatca
      361 ttttcatgcg caagcggtat ctacccgcgc gggatgagcg gtgtgattcg atatataaaa
      421 ctggcgccgg gcgccgaatt gactcatgtg tttatcagca aagatttaaa ccggaacaag
      481 agcggtcgtc gcggtaaatc cgggtaaagg tatggtgtat gtcggaaagt acgtttgagt
      541 atgtttcatg ctgcaacgtt tatctgaatt attttcgtgg cagaacgcag gataatacgt
      601 taaactattt tggggtttgg tgaatcggcg cagatcagcg gcaggtgcag tccgcgatgc
      661 tgatcatggc ggcatgatta ttttgaaaaa gcggtgtgct gatcgggcct tgatttctga
      721 cggttttccg aggggcagaa atgataaata cgataacggg aaataatctt gttgaaaagg
      781 cggcgcgggt ggcggcccat gcgcattgga ctcagaagcg caagggcgac gaccttccct
      841 acattattca tccggtgatg gtggcggtga agttaatcaa acatacgttt ccggatgcgg
      901 tgatcgccgc ggcattaacg catgatgttc tggaagatac ggattatccg gaagcgaaga
      961 tgcgggcgga actcggcgat gaggtgctgc acatagtgaa aacggtaacc catgaccggt
     1021 ctttgccgtg ggaggaaaaa aagaagaagt atattgaaac cgtgcgtgcg ggttccgaag
     1081 gcgccagggc tgttgccgtg tccgataaaa tccacaatct ggaaagtctg ctgctggcgt
     1141 atgaggatca ggggccggcg ttgtggaaac ggtttaatcg cggcagggat aagaagctct
     1201 ggtttgaaaa agcaatgctg caaatgctta aagaaagctg gcggcatcct ttgatcgatg
     1261 aatacgagcg cctgatcgaa aagatgacca agttgaaggt agagtaaccg ccggggaaca
     1321 cgccgtttcc cccaaagaac gtattttctg aaaaacagat cgaatttctc gtcgcaagag
     1381 cataaatctt aaaattgaca gccatcaagg gttattgtac aaaaagctat aggatttcga
     1441 aaagaggcgc ccttcattga ctgtaaaaag aaaaattatc attgccgtcg ttgttgtagc
     1501 ggtggttgcc ggtgccgcgg tgttgacgcg cgcctggttt tatgcggcgg ataaggttgc
     1561 gcagccggac agttaccggg aatacatcac caaaaccgtc agcgaaaaga tgcatcgcag
     1621 cattgcttat gacaagagta cggcctcgct gacgttgcgc aacgggttga cgtttaattt
     1681 tacccatctg gtcatcagcg aaaaggacgg cgcgtccgat tttttaaggg tggatgaagc
     1741 gctggtccgg atcaatattc tgccgctttt gagaaaccgt ctggttttcg gggagcttgt
     1801 tctcgatcgt ccccgtgttg ctttaaagcg cgacagctcc ggcgtgctga acatcgcgga
     1861 tcttctgata cgcaaagaag ataagaaggc gccgagaatc cggaaagtca tcattaaaaa
     1921 aggggcggtc acttttgtgg atcaggcggc ggacccggcc ggtcttgtga cgtcgctgga
     1981 aaatctcgac ggccggatta acgcgctgtt ctggatgaac cgataccgtt ttcatatcac
     2041 atcgtctatt ctggaaaaca acaacagggc gtcggtggaa ttgagcggtc tgttcagtcc
     2101 cgtctctgca ggaaagcccg tgtatgaaag taaattgcgg gcgtctgttc atttaaagga
     2161 tgcggattta aaacactaca atgcgtatct gaaaacgtac acgcctatcg agcaggtgtc
     2221 cggcaaatta aaactggata taacgtatac ggggcggttt tcagatttta aatcgaaagg
     2281 gacggttaca ttgagaaatg cgtcgcttta ttatcccggc gtttttaacg ggtatgtccg
     2341 gccgcggatt gcggaagcaa atttttcttt gaaacgcgat aagaaaagtc tgagcgtgga
     2401 tgtcggaagg ctgacggttg ataaattcaa cgcgaagggc agctttgaga tggacgatct
     2461 ggataagaag gacccgcttt tggaagcgtc ggccatcacc accgcgttcc agttgaaaga
     2521 cgttacatcc tatgttccgt ggggcattat tcacaaaggc gtgggggatt tcattcagga
     2581 gcacatcaag gatggcaagt tccggctcat tgaagggaaa ttaaaaggcc ggctgagtca
     2641 gatcgccaac atcaatcatc aggaaggcgt cgatgtgctt tccattaccg ccgaggtgga
     2701 taaaggtgtc tttgaggtgg tgccttccgc gccattcttt aatgacatca aaggcattct
     2761 ggagctcaag agaaggcagt tttccatcaa aaacatccgg gcctatttcg gcaagtcgcc
     2821 ctgcacggtg gaaggcaata tttccgattt cgcccggccc gagcccaata tttataccgc
     2881 ttacatgaag gttcagccgg ttcgcgatga tgtggtctgg ctgctgggcg agaaaacctt
     2941 caacgatatg gattttcggg ggacctcgct gttgacgctt tcgggcaaag gtccggatac
     3001 cggtttccgg atcagggccg actggaatct gacggatgtt gcttacgcat ttccgggtgt
     3061 tctggaaaaa ccgcgctcca gaaaaaatca gtttacggcc gatctggtga ttgatgacca
     3121 ggcggttgac tttcaatcgt ttaagcatgt gatgccgccg gtgaatgtta cggggaatat
     3181 gaggctgcgc tatgaggggg actcgcctgc atcgttttcc attcagtcgg ggacgtttga
     3241 tatccgtgaa gccgcaccgc tgctgccgtt attgcaaaaa ttcgatccga cgggcgcggc
     3301 gcagattgct gtgaccggtc agggtaatct caacgagccg tcttcgttgc agtggaaggg
     3361 gaacattgct ctgaaaaaca tttcgctgga tgtgtccgac aagttcagcc ggatgcaggg
     3421 gctgacgggc agtgtgtctt tcaaaaaccg gtcgatgcag acctcgctgt ttagaacgca
     3481 aatcgggcgc agtaaaattc agggcaactt ctggattgaa gatttcagcg attccagagt
     3541 gttctgccgg gtcaacagcg aggcgctcaa agcgtcggat ctgggcctga aaagcgcaaa
     3601 aggcgatgtg acgctgcgcg atgtcaaggg acaattatcc tttgaagatg aactgattca
     3661 tgtggagcgt ctgcaattcg gagcagggac gtcaaccttt attctgtcgg gagatattac
     3721 ggagttcgat ctgccgaaag ttacgctgga actgagttcc atgtatgtcc attccgagga
     3781 tttgagccgt ctgatggctt tgagttatcc gtcgaaagat caaaaggcaa aatcaaccgt
     3841 ggacctgaat gccgtcttcc tgctggatgg cgggaccttc ggcgatgcgc cgttccagaa
     3901 actgtacgcg gtcatgaagc tgaagaacga tattctggat gttgatgaac tggaagtggg
     3961 aatctttgac gggaagttca aggccaaggg cagggtggtt ttgccggcgg caggcccgaa
     4021 ccgttatgac gcgaatatca cggtcgataa aatatcgctg gataaacttc aacagtatct
     4081 ggaattgggc gatcgcacgg tgaccggaga tttatccctg tccgccaata tctcgatgag
     4141 cggcgccaat gccggggaac tcaaacagtc tgcgatggga accgtgaagg tgcgtgccga
     4201 aaaaggggtg ttgaaaaagt ttgcggttct gtcaaaaata ttttcattgc tgaatattgt
     4261 gcaactggcc aagctgaagc tgccggatat ggcaaccggc ggcatgccct acagtgagat
     4321 cacgtttcat gcgacgttca agggcggcgt gatatcctcg gaagactttt ttattgacag
     4381 caacgcgatg cagatttcgg ggacaggcag cattgatttt gtccggaaga agctcgattt
     4441 tgttgccggt gttcatcctc tgcaatcgct ggatctgatc gcttcgaaaa ttccgattgc
     4501 cgggtggatt attaccgatg agcggggaaa attaattacg gctcatttca aagtggatgg
     4561 aacctgggac gatccgaagg tgtccaccat cacggcgaaa tccatcggca aaggcacgct
     4621 cgatattttc cgccgaattt tccagttgcc ggaaaagctc gtcacggata cgggcgaagt
     4681 cattctgggg cattaaaaat gtttgccgaa acgaaacatc ggaagaaaaa ttttcaggat
     4741 ctggctgtct tgccgtaggc gttatccagt ggcgcctgat cgcgtcctct ttgcgcaatg
     4801 acaattttgg cccccgggtt ttcccggcgg gaataaataa tcagcgtgcg gaccagactt
     4861 tttctgaatc tgcctcgttc cgcttcgtca agcagcaaat caatggcggc tctcagcgaa
     4921 aagggttttc tcctgtaggg cctttccgag atgagcgcat ccagcgcatc caccaccccg
     4981 atcaactggg aatatttgtt gagggttttt atgcccagcg gataaccgga cccgtcgagc
     5041 cgttcatgat gctgaaaaga agaaaaagaa tatttcttgt gatccttgcc gaaataataa
     5101 tgcagcagaa cataaccgat caacggatgc gaggtcagga tccggcgttc cttgatggtg
     5161 atcggcgtcg gtttgtccag gatgctcaac gggatgcgcg atttgcccag gtcgtggaaa
     5221 aggctcagca gtacggctag atcgtgcatg tatttgtttt tcagcgaatg atcgaaggat
     5281 accttgccgg ccagaatggc aatgaccatg atgtgctggt atgtataggg ggagcgtgtt
     5341 ttcaaataag ccagctcgga taatatcttt tccggcatcc ggattcgggc gatcgagcgg
     5401 acgatcttcc ggttgatgtc cggaggataa agaatattgg tgtaacgttc gtctttgaag
     5461 gtctcgacaa gatccttcat cagccaggtg tctttgatcg ggacatacgt tatttttcct
     5521 gataacgacg ctacccgttg caaggttttt ctgctgatgg tttcaccggc ttgaaccaga
     5581 agcgttttgt cgatggcata caaatctttt tccagtttca tatgtcttct ccgatgacga
     5641 taggcatagt tgatttggtt ttttctgtca aatactttgc cggtgatttt taaaaattct
     5701 ccggacggtg cgtcaaaaaa aattgatttt aagaggcaag atcagtataa ggtttgtcgg
     5761 tctgatgttg aaatgaactg tcggcgttgt cgatgaaaga ttaaacgagg agcatgccat
     5821 gaatgagatg aaaaaggatc gtgaacattg ggcgagtcgc gtcggtttta ttctggcggc
     5881 ggcaggatcc gctgtcgggc tgggcaatat ctggcggttt ccctacatga cgggagaaaa
     5941 cggcggagcg gcgtttattg tgatttatct gctggccgtt tttttactcg gttatccttt
     6001 gatggtcaat gaaacggtac tgggacgcgc ctcacagcgc gatcctgtcg gcgcattcaa
//
LOCUS       DAONQZ010000102         5862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14206_length_5862_cov_15.301188, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000102 DAONQZ010000000
VERSION     DAONQZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_5862_cov_15.301188"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLR13_11030"
     CDS             <1..356
                     /locus_tag="PLR13_11030"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQC19915.1"
                     /translation="FARIYVDFFLSDKELFRILMTFMMQTGQMHLTQQQSDELIRSTN
                     ENIKVISEIIQKGVDSGEFRPLDNILQMQNAIWGMLNGVISLFLFTGNPIKRMERIHS
                     TVQESMNVFIRGLKA"
     gene            complement(431..772)
                     /locus_tag="PLR13_11035"
     CDS             complement(431..772)
                     /locus_tag="PLR13_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQC19916.1"
                     /translation="MEIKYKIWIEENGKVIFGKGRDEILKSIEQQHSLNAAAKELGMS
                     YRAAWGRLKASEERMEKKLVETSEKEKALQLTAQARTIIERFEKLEKDVENILKEAEQ
                     EFKKLFQTMGK"
     gene            996..1412
                     /gene="nikR"
                     /locus_tag="PLR13_11040"
     CDS             996..1412
                     /gene="nikR"
                     /locus_tag="PLR13_11040"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HQC19917.1"
                     /translation="MPGLVRFGVSLEEDLLVKFDRHIKQKKYTNRSEAIRDMIREELV
                     RKEWTENKEVTGAITLVYDHHTRELVTKVLDVQHDYHTCILSTQHIHLDHHNCFEIVV
                     TRGKSKEIEELFQKLKSIKSVKHAGFMMATKGKDLP"
     gene            complement(1427..2161)
                     /locus_tag="PLR13_11045"
     CDS             complement(1427..2161)
                     /locus_tag="PLR13_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQC19918.1"
                     /translation="MSHHIVEFKNVSFRYPDGTQALQDISFRITHGESVGIIGANGAG
                     KSTLLMHLNGYLMPTSGTVNIGDVYLTKKTQPEIRRKVGIVFQNPDDQLFMPTVYEDV
                     AFGPLNLGLDETAVRERVEFALHLVNSLDLKNKPPHHLSCGQKSAVAIASVMAMEPDI
                     LAMDEPAANLDPRSRRLLINLLKTFEHSKIIASHDLDLILDVCHRCIVIKEGRVAADG
                     PVSEILTHQELLEENNLELPLSMQKR"
     gene            complement(2158..2967)
                     /gene="cbiQ"
                     /locus_tag="PLR13_11050"
     CDS             complement(2158..2967)
                     /gene="cbiQ"
                     /locus_tag="PLR13_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HQC19919.1"
                     /translation="MSFDIQYYNIGFLDQLSYKSTFVHRLDPRVKLCVTLLFLLTVIS
                     FSKYEIFALLPFFLFPVLLMTLGEIPFMFIFKKVLLVSPFAVFIGIFNPLLDSGQISI
                     VNGYTISAGWLSFISILLKFTLTVSTALLLVATTSFPGVCHALRQLGLPSVFVSQLLF
                     LYRYIFVLMEETMRIVRARNMRSFGDRGKGIKIFVRIAGTLFLRTVQRAERVYNAMLS
                     RGFQGDMPALKHFQFKTGDMIFAATVAAFLLIFRFCPATEILGRFAQGVFS"
     gene            complement(3070..4098)
                     /locus_tag="PLR13_11055"
     CDS             complement(3070..4098)
                     /locus_tag="PLR13_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HQC19920.1"
                     /translation="MADALLSPAVGATMWAGSVAAIGYSSKKLKENIDNKTIPLMGVM
                     GAFVFAAQMINFTIPVTGSSGHIGGGMLLAVLLGPYAAFLAMASILTVQALFFADGGL
                     LALGCNIWNMGIYPCFLVYPLIYKPVAGDGKSARRVSVAAILGAIVALQLGAFSVVLE
                     TLFSGISELPFGTFVLLMQPIHLAIGLVEGIITAGIILYVQQARPEILHAHAAARPLD
                     AGLSIKNILAAFVILAVITGGALSWFASTHPDGLEWSIEKVTGKGELEEATQGIAPVL
                     KDIQEKTAFLPDYGFKPPADEQNTEESPSVWPGIEPGTSVAGVVGSAIVLMFIIFIGF
                     GIRLFKKQ"
     gene            complement(4149..4931)
                     /locus_tag="PLR13_11060"
     CDS             complement(4149..4931)
                     /locus_tag="PLR13_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQC19921.1"
                     /translation="MIRKHFINGLVWAVLFFIAGPSWAAHPLITDDTGTQGKGNFQLE
                     LTGQYDQDKENVGGVSIKATGVETAATLSYGMIDNIDLVLSLPYLWGREKENGSLVYK
                     EDGIGDASLEAKIKVFEKEGFSLALKPGISFPTGDDDKGLGTGKFGGQIFFIASQEVE
                     PFAFHVNLGYIRNENDADERENIWHASVAAAWEIVKDLSLVANIGVERNPDGEADDDP
                     AFLIGGIIYSIAENLDIDLGVKYGLTDSEADISALAGLAFRF"
     gene            5144..>5862
                     /locus_tag="PLR13_11065"
     CDS             5144..>5862
                     /locus_tag="PLR13_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5729..5731,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQC19922.1"
                     /translation="MHISRRGFLTLSSAVGGGIALSSLGLNLKPTRAYADELDKMNRV
                     KAAKQTTTVCCYCSVGCGLVCSTDKATGKIINIEGDAEHPINEGSLCAKGAGFFDLTE
                     ANKHRLKKVLYRKPYGTEWEEKDWNWALPRIARLIKDTRDKGFVTKNAKGELVNRCET
                     IGHYGSSNVDNEECWLMSVKCRALGLVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIA"
BASE COUNT         1552 a         1426 c         1447 g         1437 t
ORIGIN      
        1 agtttgcccg tatttatgtc gatttctttc tcagcgacaa ggaacttttc agaattctga
       61 tgacgttcat gatgcaaacg gggcagatgc acttgacgca gcagcaaagc gatgagctga
      121 tccggtcgac gaatgaaaac atcaaagtta tttcggaaat tattcaaaaa ggagtcgatt
      181 ccggtgaatt cagaccgctg gacaatatct tgcagatgca gaacgccatc tggggaatgt
      241 tgaacggtgt tatttcactt ttccttttca ccggcaatcc gatcaaaaga atggagagaa
      301 ttcactctac cgttcaggaa agcatgaatg ttttcattag ggggttgaag gcgtaatttt
      361 tccggaatgc aaggtgcgca atccgaatgc cttgcagttc agtgcagggc ttgaaccggg
      421 tatttcgggt ttacttcccc atggtttgaa ataatttttt aaattcctgc tcggcctctt
      481 ttaatatgtt ttccacatct ttttccagtt tctcgaatct ttcgataatg gttctcgcct
      541 gagccgtcag ttgcagggct ttttcttttt cgctggtttc caccagtttt ttttccatgc
      601 gttcttccga cgctttcagt ctgccccagg ccgcacggta tgacattccc agttccttgg
      661 cggcggcatt gaggctgtgc tgctgttcga tggatttgag aatttcatcg cggcctttgc
      721 cgaaaatgac cttgccgttt tcttcaatcc agattttata tttgatttcc atcggtgcct
      781 cccccccagg ctattgaatc ttgttttgtt ataattaaca tacttacaat atgtcaataa
      841 aattcgcaac cgccacagcg ccgtaaaacg taaactgtga accggttgaa gaattggaga
      901 agaagacaga tgctctcagg ccggcataaa tcatcttgac tattcaatga cgttgcctgt
      961 atttacataa aaaaatcact gaaggagttg atcgtatgcc cggactggtt cgatttgggg
     1021 tatcgctgga agaagatttg ctggttaaat tcgaccggca tatcaagcag aagaaataca
     1081 ccaaccgttc cgaagccatc cgcgacatga tccgcgagga actggtccgg aaagaatgga
     1141 cggaaaacaa ggaagtgacc ggcgcaatca cgctggttta tgatcatcac accagggaat
     1201 tagtcaccaa agtgcttgat gtgcagcatg attatcatac ctgcattctt tccacgcagc
     1261 atattcatct cgatcatcat aattgttttg aaattgttgt gacccggggc aagtccaaag
     1321 aaattgagga gctctttcag aaactcaaat cgatcaaaag cgtcaaacat gccggattca
     1381 tgatggcaac caaggggaag gatttaccct gatgcgttta aaggggttat cgcttctgca
     1441 tggataaggg cagttccaga ttattttctt ccagcagttc ctgatgggtg aggatctccg
     1501 aaaccgggcc gtcggcggcc acccttcctt ctttaatgac aatacaccga tgacagacat
     1561 ccagaatcag atccagatca tgtgaggcga tgatcttcga atgctcgaag gttttcagca
     1621 gattgatcag caggcgccgt gatcggggat cgagattggc cgccggttcg tccatggcca
     1681 gaatatccgg ctccatggcc atgacggacg caatggcgac ggcgctcttc tggccgcagg
     1741 agagatgatg aggaggtttg tttttcaggt ccaggctgtt gaccagatgc agggcgaatt
     1801 ccacccgttc ccgcacggcg gtttcatcca agcccaggtt tagcggtccg aaagccacgt
     1861 cttcatacac ggtcggcata aagagttgat cgtcgggatt ttgaaaaacg ataccgacct
     1921 ttctgcggat ttccggttgc gtcttcttcg tcagataaac atcgccgata ttcaccgtgc
     1981 ccgatgtcgg catcaggtag ccgttgaggt gcatcagcag cgtagatttg cccgcaccgt
     2041 tggcgccgat gattcccacc gactccccgt gcgtgatccg gaaagaaata tcctggaggg
     2101 cttgggttcc gtccggatag cggaaagaaa catttttaaa ctcgacgata tggtgactca
     2161 tgaaaaaact ccttgtgcaa accggcccag aatttcggtt gccggacaga accggaagat
     2221 caacagaaac gccgcaaccg tcgcggcaaa aatcatgtcg ccggttttga actgaaaatg
     2281 tttgagcgcg ggcatatctc cctgaaagcc ccgggacaac atggcgttat agacccgttc
     2341 cgcgcgctgc accgtccgca aaaaaagcgt tcccgcaatg cggacaaaga ttttgatgcc
     2401 cttgcctcgg tctccgaaag agcgcatatt tctggcccgg acaatccgca tggtttcctc
     2461 catcaggaca aaaatatagc gataaagaaa aagaagctgt gaaacaaaaa ccgaaggcag
     2521 ccccaactgc ctcaacgcgt ggcagacacc gggaaaagag gtggtggcga caagcagcag
     2581 agccgtgctg acggtaagcg tgaacttgag caggatggaa ataaaagaca gccatcctgc
     2641 cgaaatcgta tatccgttga ctatcgatat ctgtcccgaa tccagcagcg gattgaagat
     2701 gccgataaaa acggcgaaag gtgaaacgag aaggactttt ttaaagataa acataaacgg
     2761 aatctcgccc agcgtcatca gaagcaccgg gaaaagaaaa aaaggcagca gagcaaaaat
     2821 ttcgtatttg gaaaaggaaa tgaccgtaag cagaaaaagc agggtaacac atagtttgac
     2881 ccgtggatcc agccggtgca caaaggtgct cttgtaggaa agctgatcga ggaagccgat
     2941 attatagtat tgaatgtcaa atgacatgat ttggcccgat aatggcttta agaattgatt
     3001 ctagatatca tacgccctga caggaaatcc agctattttc aaggcgtatg ccgggtatga
     3061 tgacaaaagt tattgttttt taaaaagccg tatgccgaaa ccgatgaaaa tgatgaacat
     3121 caacacaatg gccgatccga caactccggc gacagatgtt ccgggttcaa tgcccggcca
     3181 gaccgagggc gactcctccg tgttttgttc gtccgcgggc ggtttgaaac cataatctgg
     3241 gagaaatgcc gtcttttcct gaatatcttt gaggacaggc gcaatgccct gtgttgcctc
     3301 ttccagctcg cccttgccgg taactttctc aatggaccac tccagcccgt cgggatgcgt
     3361 tgaggcaaac caggaaagcg caccgccggt aatgaccgcc agaatcacaa atgctgcaag
     3421 aatgttcttg atggaaagac cggcatccag gggacgggcg gcggcatggg catgcagaat
     3481 ttccggccgg gcctgttgca catacaggat gatgccggcc gtgatgattc cctccaccag
     3541 gccgatggcc agatgaatcg gctgcatcag caggacaaac gtgccgaagg ggagttcgct
     3601 gattccggag aatagcgttt ccagaaccac cgaaaaagcg cccaactgca gggcaacgat
     3661 tgcgccaagt attgcggcaa cggatacccg tctggcgctt tttccgtctc cggcaacagg
     3721 tttgtaaatc aggggataaa ccagaaagca gggatagatg cccatgttcc agatattaca
     3781 gccaagcgcg agtagcccgc cgtcggcaaa gaacagcgcc tgaaccgtca atatcgaggc
     3841 catggccaga aaagcggcgt agggacccag aagaacagcc aaaagcatac cgcctccgat
     3901 atgtccgctg gaacctgtga cgggaatggt gaagttgatc atctgcgccg caaagacaaa
     3961 cgcgcccatg acgcccatca gcgggatggt tttgttatcg atattttctt tgagcttttt
     4021 tgatgaatat ccgatggcgg ccacggaacc ggcccacatc gttgctccca ccgcgggtga
     4081 gagtaaagca tccgccatat gcatgttaaa ttctcctttt aaaaaagatc agttatccgc
     4141 cttcctctct agaaccggaa tgcaagtccg gcgagcgcag aaatatcggc ttccgagtct
     4201 gtcaaaccgt atttcacgcc gaggtcgatg tccaaatttt ctgcgatcga ataaataatc
     4261 ccgccgataa gaaacgcagg atcgtcatcc gcttcgccat caggattgcg ctcaacgccg
     4321 atattggcga ccagacttaa gtctttgaca atttcccagg cagcggcaac ggaggcatgc
     4381 cagatattct cgcgctcgtc ggcgtcgttt tcatttctga tgtatcccag attaacatga
     4441 aacgcgaacg gttccacttc ctgggatgca atgaaaaaaa tctgacctcc aaattttcct
     4501 gtgcccagtc ccttgtcatc atcgcccgtg gggaaactga ttcccggttt caacgccagg
     4561 ctgaagcctt ctttttcaaa gaccttgatt tttgcttcta atgaagcatc gccgatgccg
     4621 tcttccttat atacaaggct gccattttct ttttctcttc cccaaagata aggcaggctt
     4681 aataccagat caatattatc tatcatcccg taagacaatg tggctgccgt ctcaactccc
     4741 gttgctttaa tcgaaacacc accgacattt tctttgtcct gatcatattg tccggtcaat
     4801 tccaattgaa aatttccctt cccctgcgtg ccggtgtcgt cggtaatcag gggatgcgcc
     4861 gcccaggatg gaccggcgat gaaaaacagc actgcccata ccaatccgtt tataaaatgt
     4921 tttctgatca ttttctcctt ccatttgtgc tacgattata attttagtgg cacgatatgg
     4981 cggttctgtc aagcttttaa tgattttaaa aagcaataaa ccctattaaa ccggcttcat
     5041 taaaatatgt tttatataac atattgactt ttgtatgact ttcggtcaat ctatgccacg
     5101 tttgacatat accaaaactg acataggtag tggggaggat tttatgcata tttcaagaag
     5161 aggtttttta actctttcca gtgccgtcgg aggcgggatt gccctgtcca gcttggggct
     5221 taatctgaag ccgacgcggg catatgcgga cgagttagac aagatgaaca gagtgaaggc
     5281 cgccaaacag acgacgacgg tttgctgtta ctgttccgtc ggctgcggtc tggtttgcag
     5341 cacggacaag gcgaccggaa aaatcattaa cattgaaggc gacgccgagc atcccatcaa
     5401 tgaaggatcg ctttgcgcca aaggtgccgg tttcttcgat ctgaccgaag ccaacaagca
     5461 caggctaaaa aaggttctct atcgcaagcc gtatggaacg gaatgggaag agaaagactg
     5521 gaactgggcg ctgcccagga ttgcccgctt gattaaagac actcgtgaca aaggttttgt
     5581 aacgaagaac gccaagggcg aactggtcaa tcgctgcgaa acaatcggcc actacggcag
     5641 ttccaacgta gataatgaag aatgttggct gatgagcgtc aaatgccgtg ctctgggttt
     5701 ggtttatatc gatcaccagg ccagggtctg acacagccca tctgtagcgg ctctgggaga
     5761 gtcgttcgga cgcggttcca tgacgaacca ctactgtgat ttgaaaaacg cggatgtcat
     5821 tttgattatg ggcagcaatg ccgcggaaca tcaccccatc gc
//
LOCUS       DAONQZ010000103         5775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14472_length_5775_cov_16.216783, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000103 DAONQZ010000000
VERSION     DAONQZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_5775_cov_16.216783"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..984)
                     /locus_tag="PLR13_11070"
     CDS             complement(298..984)
                     /locus_tag="PLR13_11070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQC19923.1"
                     /translation="MASLKIRQTTIKAVIFDFDGTLAKLTIDFSKMRSDIEALVASYR
                     IIPQELKTTFVLETIVAAHDLIRRQSAQQADAFFSRANRMIEDIETQGAQNGHLFGWT
                     KDLLESLQAAKISCGIITRNCARAVNMVFPDILTYCPVVVCRDDVRRVKPHPEHIRLA
                     LDRLGALPDNTLMVGDHPIDITTGRNARTLTCGVLTGRCPKENFVAAGADLVLDNAAE
                     ILSILKIADG"
     gene            complement(989..1831)
                     /locus_tag="PLR13_11075"
     CDS             complement(989..1831)
                     /locus_tag="PLR13_11075"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQC19924.1"
                     /translation="MDLNLFQNKVALSRLIVEKLAALPVDFKEKDAEIDASRKQGSAH
                     LKAAVVLPLYFKEFNGRSEYVFQLIKRSESISQGGDISCPGGMLQPRQDNMLSSFLKR
                     QMLPALGFRITGHIQHADADTDRLIRLFLTTALREAWEEIGLNPLNAKLLGALPSYSL
                     TFLSRTIFPLVCRIRRPYSYRLSSEVEKVLEIPLSFFFESENYATLDVHMTVGESNEP
                     FCYQTPCLVIPDAGGNNDILWGATFNIIRNFLQVISGGTVPEATSARMMTKTLTNRYI
                     SPRG"
     gene            complement(1844..2827)
                     /locus_tag="PLR13_11080"
     CDS             complement(1844..2827)
                     /locus_tag="PLR13_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQC19925.1"
                     /translation="MDEKTNITDDPFEPALFLRNPHVQSIMASSRLRLHYHWRNELAR
                     NTEEVILKTSTGSRLVSYLTRHPEPKGMFVFLHGWEGSSSSIYILDSADYFYRRGYMI
                     GRLNLRDHGDSHHLNEDLFHAARLKETFDGVDYLASFSRIKPVYLVGFSLGGNFAMRI
                     ALRHGMKPINHLEHVFAISPPMNPYKATMAIDNGRAFYRYYFLKKWKRSLMKKQKLFP
                     DRYDFSDLMDVESCMELTKMLIPYFPEIQSYQHYFKMYTLKYNAFKYLSLPLTVLIAE
                     DDPIVSYQDFLNMDANHNLKILKQRYGGHCGFLDLFPASCWYQKAIAAIVS"
     assembly_gap    3511..3610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3707..4984)
                     /locus_tag="PLR13_11085"
     CDS             complement(3707..4984)
                     /locus_tag="PLR13_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC19926.1"
                     /translation="MKKFIFLSIILSLFAVFSVAQAEVRDRSFSLTPYVGGYFYEGNQ
                     DLKDSALFGLRAGYNFTRHFGIEGFASYTQTEIDDSYYSGTSPWQNIYNFGVEAIYHF
                     LPEKRFVPFVAMGLGGVHYEEARNYKSHYGAPDYGKIFEATKLAVDYGAGMKYFVTDN
                     IALRLDVRHILPLNGRWNNPHYIHNELQATFGVNFAFGGGPKVAVQEAAVEEAPVQPV
                     VSAADGDKDGVPDFRDKCPKTPAGRTVDADGCMPDSDGDGILDYEDHCANTPAGVKVD
                     ADGCPSDSDFDGVSDYRDKCPGTPHGSPVDEDGCLFKKTSMFLKIEFDSGKSVIKPRY
                     HQEIKRVADFMKKNSKATATIVGHTDNTGGRLANIRLSQNRADSVRQYLISKFGIAAQ
                     RIKAMGYGPDRPIAGNKTDAGRKKNRRVVASFE"
     gene            complement(5132..5476)
                     /locus_tag="PLR13_11090"
     CDS             complement(5132..5476)
                     /locus_tag="PLR13_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19927.1"
                     /translation="MEIINRLLEEELKRLGTLHDFYEKKIMESPRGSLSVKARGKNRY
                     LYLARREDKKVVFEYVGKDVAYVRHALNEQMKQRREYQAKLREVKENIKEVKRSLRTK
                     RNRDGPAAVNSS"
BASE COUNT         1443 a         1475 c         1375 g         1382 t
ORIGIN      
        1 gggcatgcgg gaatgacagt tgtgtggggc acgcgggaat gacaggggaa gcgaagcgga
       61 tgggggcaca gcggggttgt gtcacttcga acaaccatca accatcaaca atgaactgtt
      121 tctttaacct ttaaccttga accttggact ttttttaagc gcgaagcgca tcggggcaca
      181 gcggtgtttt gcctaaaacg taaaacctaa aacctaaaac ctaaaaccta aaacttaaaa
      241 cttaaaactt aaaacttaaa actattattt tctttaacct tgaacctctt ttcaccatca
      301 accgtcagct attttcaaaa tcgacaaaat ctccgctgcg ttatccagta ccagatccgc
      361 gccggcggcg acgaaatttt ctttcggaca ccggccggtg agcacgccgc aggtcagcgt
      421 ccgggcgttt cgtcccgtcg tgatgtcgat gggatgatcc ccgaccatca gcgtgttgtc
      481 gggcagggcg ccgagtctgt ccagcgccag acggatgtgt tccggatgcg gtttgaccct
      541 gcgcacatcg tcgcggcaga ccaccacggg gcagtaggtc agaatatccg ggaaaaccat
      601 gttgaccgcg cgggcgcagt ttcgggtgat aatcccgcag gaaatttttg ccgcctgaag
      661 cgattccagt agatcttttg tccagccgaa cagatggccg ttttgcgccc cctgtgtttc
      721 gatatcttca atcatccggt tggcccgtga aaaaaacgcg tccgcctgct gcgccgattg
      781 ccgccggatc aggtcatggg cggcaacgat cgtttccaga acaaaggttg tcttcagctc
      841 ttgaggtata atccggtacg acgcaaccag cgcctcgatg tccgagcgca ttttggaaaa
      901 gtcgatggtc agctttgcca gcgtgccgtc gaaatcgaaa atgaccgctt tgatcgttgt
      961 ctgtctgatt ttcagcgatg ccataacatc agccccgggg agaaatataa cggttggtca
     1021 gcgtcttggt catcattcgg gcggacgtcg cctccggaac agtgcctccg gagatcacct
     1081 gaagaaaatt gcggatgata ttgaaggtcg ctccccagag aatatcgtta ttgccgccgg
     1141 catcgggaat caccaggcag ggcgtctgat agcaaaacgg ctcgttgctt tcacccacgg
     1201 tcatgtggac gtcgagcgtg gcgtagtttt cgctctcgaa gaaaaagctt aaagggattt
     1261 ccagcacctt ttccacttcg gaactcagcc tgtatgaata cgggcggcgg atgcggcata
     1321 ccaacgggaa aatcgtccgg gacagaaacg tcagagagta ggagggcagg gcgcccagga
     1381 gttttgcgtt cagcggattc agaccgattt cctcccaggc ttcgcgcagc gcggtcgtca
     1441 gaaaaagccg gatgagacgg tcggtgtccg catcggcatg ctgaatgtgt ccggtaattc
     1501 tgaagcccag cgccggcagc atttgccttt tgagaaaaga actcagcata ttatcctgac
     1561 ggggttgcag catcccgccg ggacaactga tgtcgccgcc ctgcgaaata ctttccgagc
     1621 gcttgatcaa ctgaaaaaca tattcggagc ggccgttgaa ttctttgaaa tacaaaggca
     1681 gaaccaccgc cgccttgaga tgagcggacc cctgtttgcg ggatgcatcg atctcggcat
     1741 ctttttcctt gaaatctacc ggcagcgccg ccagtttttc gacgatcagg cggctcaacg
     1801 caactttatt ctgaaaaagg ttcaagtcca taggctgtga aaactacgaa acgatagcgg
     1861 caatggcctt ctgataccag cacgacgcag ggaacaaatc cagaaacccg cagtgcccgc
     1921 cgtaccgctg ttttaaaatt ttaagattgt gatttgcatc catattgaga aaatcctgat
     1981 aagaaacaat cggatcgtct tcggcaatca gcaccgtgag cggcagcgac agatatttaa
     2041 acgcgttata tttgagcgtg tacatcttga aatagtgctg gtagctctgg atctcgggaa
     2101 aatacgggat cagcattttc gtcagttcca tgcagctctc cacgtccatc agatcgctga
     2161 aatcgtaccg gtccggaaaa agtttttgtt ttttcatcag ggaccgcttc cattttttca
     2221 gaaaataata ccggtagaag gcgcggccgt tatcgatggc catggttgct ttgtagggat
     2281 tcatcggcgg gctgatggcg aacacgtgtt cgagatgatt gatcggtttc ataccgtggc
     2341 gcagggcgat gcgcattgcg aaattgccgc ccagcgaaaa cccgacaaga taaaccggct
     2401 tgattctgga aaatgaggcc agataatcca ccccgtcgaa cgtttctttc aggcgggcgg
     2461 cgtggaagag atcttcgtta agatgatgcg aatcgccgtg gtcgcgcaga ttcaggcggc
     2521 cgatcatata gccccggcgg taaaaataat cggcggaatc cagaatatat atggaagacg
     2581 acgaaccttc ccagccgtgt aggaaaacga acatgccttt cggctcggga tggcgggtga
     2641 gataagacac cagccgggaa ccggtggacg tttttaaaat aacctcctcg gtgtttcttg
     2701 ccagctcgtt gcgccagtga tagtgcaacc ggagacggga actggccatg atcgactgga
     2761 catgcggatt gcgcaaaaac aaagcgggct caaacggatc atccgtgatg ttggtctttt
     2821 catccataag cagtgataaa acattgaatc ctgattcata aaatcaaaaa attggaattt
     2881 aagcaagcgc ataatttccc cggcccgctt gccggaagcc gtcgcgccgc gggccataaa
     2941 cattgaactc actgttcccc atcagctgcg ctccttaaaa agttcaaggt tcaaggttca
     3001 aagttcaaag ttcaaagtta aaaacactgt tgtccccatt cgctttcctt cccctgtcat
     3061 tcccgcatgt ccctggcggg aatccagcac caccgtcccc atccgctacg cttcggggat
     3121 aattcgcctt acgcttcgac cagcgcttcg cttgcttccc tacgtcgcta cgctcctagg
     3181 atagttcggc taacgtttca atcatcgttg cactcgattt caacgagtcc tcactatcag
     3241 cttggctcat tatcattccg gcatgggcct agccggaatc cagaaaagaa taaatactag
     3301 atctacccag agggcaccat ggcccgacaa acccactcgg ggatgacaaa aggtgtggcc
     3361 cactcaggga tgagaatgtt gtatccattc gggaattaca ggggaaccgt gtcccttcga
     3421 acattccttt gtcacaacat cgagagtatg tcatttcgaa cgcatgtgcg aagccttgtg
     3481 ttttttacct aaaacctaaa acttaaaact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn cggagagacg ggaaatctaa tgacagctag ccttccggca tcgtaacgac
     3661 accggaaggc tctgattcgg gagaatcgga aaactacagc aaaaatctat tcaaaagaag
     3721 cgaccactct gcggtttttc ttgcgtcctg catctgtttt gttgccggca atcggtctgt
     3781 ccggtccata tcccatggct ttgatgcgtt gggcggcaat gccgaacttg ctgatcagat
     3841 actgccgcac gctgtcggcg cggttctgcg acagccggat attggccagg cggccgccgg
     3901 tgttatccgt atggccgacg atggttgccg ttgccttcga attcttcttc ataaagtctg
     3961 ccaccctctt gatttcctga tgatacctcg gcttgatcac cgatttgccg gaatcgaatt
     4021 cgattttcag gaacatcgag gtctttttga aaagacagcc gtcctcatca acgggcgaac
     4081 cgtggggcgt gccggggcac ttatcccgat aatcggaaac gccgtcaaaa tcggaatccg
     4141 agggacagcc gtcagcgtca accttcacgc cggccggcgt attggcgcag tgatcctcgt
     4201 aatcgagaat gccgtctcca tccgaatccg gcatgcagcc gtctgcatca accgtgcggc
     4261 ccgcaggcgt cttgggacac ttatcccgga aatcgggaac gccgtcctta tcgccgtcgg
     4321 cagcgctgac caccggctgt acgggcgctt cttcaacggc ggcttcctgc actgcaacct
     4381 taggtcctcc gccgaaggca aagttaacgc cgaacgtcgc ctgtagttca ttgtggatgt
     4441 agtgcggatt attccagcgt ccattcaggg gcagaatatg gcgcacatca agccgcagcg
     4501 cgatattatc cgtcacaaaa tatttcatgc ccgcgccgta gtcgacggcc agtttggtgg
     4561 cctcgaaaat tttgccgtag tcgggagcac cgtaatgcga cttgtaattt ctggcttctt
     4621 cataatgaac cccgcccagc cccatggcga caaacggaac aaaacgtttt tccggcagga
     4681 aatgataaat cgcctccacg ccgaaattat aaatgttttg ccagggggat gtgccggaat
     4741 aatacgaatc atcgatctcg gtctgggtat aactggcaaa cccttcaatc ccgaaatgcc
     4801 tggtaaaatt ataaccggcg cgcaaaccga acagggcgga atctttcaag tcctgattgc
     4861 cctcatagaa ataaccgccc acatacggcg tgagtgaaaa ggacctgtcc ctgacctccg
     4921 cctgtgccac cgaaaaaacc gcaaacaacg acaaaataat gcttaaaaag ataaattttt
     4981 tcataatcca ttctcccttt ttgatgatta aatttaaaat gtttatgttt gagatatcat
     5041 taaaaaaacc gttgacgatc tgaaacataa gccatccctc cttgttaaac attccatttc
     5101 ttgcccgcac gcttttcttg ccggcggccc cctaagatga atttacggct gccggaccgt
     5161 cccggtttct ttttgtgcga agcgaccgtt ttacctcctt gatattttct ttcacctcgc
     5221 gaagcttcgc ctgatactcc cggcgctgct tcatctgttc atttaacgca tgccgcacgt
     5281 aagcgacatc cttcccgaca tactcgaaga ccactttttt atcttcgcgg cgcgccagat
     5341 acaggtatcg attcttgccc ctggctttta ccgataacga tccgcggggc gattccatga
     5401 ttttcttttc ataaaaatca tgaagcgtac ccagccgttt gagttcttct tccagaagcc
     5461 gatttatgat ttccatataa tattatgcct aacaaatatg cgctattata aaactgttag
     5521 gcaaaaagtc aacaaaaatt tacctaacaa atatatttaa attaaatata agttaggcaa
     5581 ataagcccgg atggccccga aaagtcaaat tttctataaa tagcgactaa aaaatgccta
     5641 acaataataa ttaatatatg ctaaatgtta ggcaataata caataataaa aatgcctaac
     5701 attatattaa tgatgtaata aatgttaggc aattattcta agccaggtga tgacgccctg
     5761 tccgtcatac cggcg
//
LOCUS       DAONQZ010000104         5748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14557_length_5748_cov_14.453715, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000104 DAONQZ010000000
VERSION     DAONQZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14557_length_5748_cov_14.453715"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1536)
                     /locus_tag="PLR13_11095"
     CDS             complement(37..1536)
                     /locus_tag="PLR13_11095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQC19928.1"
                     /translation="MEAFSTLLLLFVCSLFLSLVLTPAVRALGIRLGAMDIPMERKMH
                     TEPIPRIGGLAVFLSFTITGMLFNFFHPFMGDLYVLNFSSFMGHLGAVVILSCGLWDD
                     FRRLNPWTKLLVQILAATCAFMGGATINSFLIGGVGFEFNTYGSYAITVFWFLLFINA
                     INLIDGLDGLAGGLIVFTCIVMTVSTFMHQDYLSAFYFAVLGGSVLGFLKYNFNPATI
                     FLGDGGSYFLGYTVAILAIRSSAKSHVGILMLIPLLALGIPIFDSILSPIRRFMLGKP
                     IFHPDKGHIHHIMMKMGLSSQRVVYMIYGITMALCILAIMIIILRDQTFEGLVLGGLL
                     LGMLILVRKLGYFEYLALDKFQGWFQDVTDVAGFSQNRRSFLALQIATSKSKNMDELW
                     HNIVEAMEMLKFHHAKLMVIDEPVREWTMPEDGEHAYFCAPSSEADLDGMLRFEIPLR
                     EYGSDTFMGKLILIKDLKKGFLKSYTIRRVEHLRRSLIPVLKKLKLKDG"
     gene            complement(1607..2671)
                     /gene="galE"
                     /locus_tag="PLR13_11100"
     CDS             complement(1607..2671)
                     /gene="galE"
                     /locus_tag="PLR13_11100"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQC19929.1"
                     /translation="MTEVLVTGGTGYIGSHTAVSLMEAGYRPVIVDNLSNSEASVVKC
                     IARITGKQPPFYKLDLRNANALNKVFEKHRIDSVIHFAGLKAVGESVDAPLLYYENNI
                     MGALSLCRVMAANRVKKIIFSSSASVYGEANIIPVTEDCPPAPTNPYARTKLMIEQIL
                     QDSHRADPAWRIILLRYFNPVGAHFSGLIGESPRGIPNNLMPFLSQVAVGRRPHINVF
                     GNDYPTPDGTGVRDYIHIMDLAEGHIAALKKIEKAAAPKGQKPLIVNLGTGKGYSVLE
                     MIRAFEKSSGQKIPYRIARRRPGDVATLYADTQLARKALGWKAKRNLADMCTDAWRWQ
                     SSHPEGYAGSSARPVKKNPG"
     assembly_gap    2908..3007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3184..3474)
                     /locus_tag="PLR13_11105"
     CDS             complement(3184..3474)
                     /locus_tag="PLR13_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQC19930.1"
                     /translation="MKKQPAVYILASAKNGTLYTGVTSDLVKRIWEHKNNLVDGFTKR
                     YSVHDLVWFELHDTMDSAINREKNIKEWKRAWKLKMIEKDNPAWRDLYIDVV"
     gene            complement(3685..4080)
                     /locus_tag="PLR13_11110"
     CDS             complement(3685..4080)
                     /locus_tag="PLR13_11110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQC19931.1"
                     /translation="MRERRKEERLKILNWVSVSVVSEETQCSERIIPFNYSENISSSG
                     MKIRGSAFLPVGTRVKIDYRMNSGSDKTITVTGKIVWIRMIIENKYYEAGVEFAQLSD
                     ETAGKLKDYIQSKIDKKDKAPFKVDLDHK"
     gene            complement(4340..5632)
                     /locus_tag="PLR13_11115"
     CDS             complement(4340..5632)
                     /locus_tag="PLR13_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC19932.1"
                     /translation="MGKTKQGKPNKLQKTKTTGKKGKTVKGAAPAGRKNPVVPLTADE
                     IYRQLFNLSPNAIAVFKPEDGTICNVNDFLCQGSGYSREEIIGRTSQDLQLFSQDIRE
                     KALACIAKDGCFRGLEVQHRLKDGSMVTCLDSGDLVKIGDEYYVIVVAQDITHIKNIE
                     EALHNEHNLNKAIINNLPGLFYVIDEYFRFQLWNDNYTRITGYTSDDLRRMSALNLAP
                     PDKRSSTVEKIKSGFDIGESSGEGEIVCKDGSFKTFLFNSRIINYKGKRCMMGTAMDI
                     TDKKKSEEDLKRFAESLEDANIALRVLINHSNNDKKMLEDKLQTNINDLVMPYIDKLK
                     NMHLDERSRKFISILETNLGSVLSPFMTDLREYHKKMTPQEMQIIDLIRKGKITKEIA
                     SILNVSVNTIATHRNNIRKKLNLRKTKSNLRSYVRSIK"
BASE COUNT         1469 a         1480 c         1223 g         1476 t
ORIGIN      
        1 aaccttgaac cttgaacatt gaactttttt tcaccatcac ccatccttga gtttcagttt
       61 tttcaaaacc ggtatcaatg atcggcgcag atgttcaaca cggcgtatcg tgtacgattt
      121 caaaaaccct tttttcagat ctttgatcaa aatcaatttg cccataaacg tatcggagcc
      181 gtattctcta agcggaattt caaacctgag catcccgtcc aggtccgctt cggaagaggg
      241 ggcgcagaag taagcatgtt cgccgtcttc cggcatcgtc cattcgcgga caggttcatc
      301 gatcaccata agttttgcgt gatgaaattt cagcatctcc atcgcttcga caatattgtg
      361 ccacaattca tccatatttt tggacttgct tgttgcaatc tgcagggcaa gaaagctgcg
      421 ccggttctgg ctgaagcccg ccacatccgt gacatcctga aaccagccct ggaacttgtc
      481 caaagccaga tactcgaaat aacccagctt gcgcaccaga ataagcattc ccaaaagaag
      541 gccgccgaga acaagccctt cgaacgtttg atcgcgcaaa ataatgatca taatagccag
      601 gatgcacagc gccatcgtga tgccgtaaat catgtagaca accctttgcg atgaaagccc
      661 catcttcatc atgatgtgat gaatatgccc tttatccgga tggaagatcg gtttgccgag
      721 cataaaacgg cggatcggcg acagaatgga atcgaaaatc ggaatgccga gcgccagcag
      781 aggaatcaac attaaaatgc ccacatgcga ttttgcggaa ctgcggatgg ccagaatggc
      841 cactgtatag ccgagaaaat aactcccgcc gtcgcccaga aaaatggtgg ccggattaaa
      901 attatatttc aaaaatccca gcaccgatcc gcccagcacg gcaaaataaa atgcggaaag
      961 ataatcctgg tgcatgaaag tcgaaactgt cattacaatg caggtgaaaa caatcagccc
     1021 gccggccagc ccgtccagcc cgtcgatcag attgatggcg ttgataaaca acaaaaacca
     1081 gaagaccgtg atcgcgtaac tgccgtacgt attaaactcg aagccgacgc ctccgatcaa
     1141 aaaactattg attgttgcgc cgcccataaa ggcgcatgtt gccgccagga tttgaaccaa
     1201 taatttcgtc cagggattaa gacgccggaa atcgtcccag aggccgcaac tcaaaatcac
     1261 aacggcgccc agatgtccca tgaaggaact gaaattcagc acgtataaat ctcccatgaa
     1321 cgggtggaag aaattgaaca gcatgcccgt gatggtgaag gaaagaaaaa cagccaggcc
     1381 gccgatgcgc ggtatcggtt cggtatgcat cttgcgctcc atgggaatat ccatcgcgcc
     1441 cagccggatc cccagcgctc gcaccgcagg cgttaaaaca agagaaagaa acaaagaaca
     1501 cacaaacaac aacaacaatg tcgaaaaagc ttccatactg ccacacacct acatcgaatc
     1561 ttatactttt tctgttacat ttacggcaaa cattgccgct tcaactttac cccggatttt
     1621 ttttcaccgg gcgtgcggag gaaccggcat acccttcggg atgcgaagac tgccagcgcc
     1681 acgcatcggt gcacatatcc gccaaatttc ttttcgcctt ccagcccagc gccttgcgcg
     1741 ccagttgagt atcggcataa agcgttgcca catcgccggg ccgccgccgg gcgatgcgat
     1801 acggtatttt ttgaccggac gatttttcaa acgcccggat catctccaga acggaatacc
     1861 ctttgcccgt tccgagattc acaatcagcg gcttttgtcc tttgggcgcc gccgcctttt
     1921 ctattttttt caatgcggca atatggcctt ccgccagatc cataatatga atatagtcgc
     1981 ggacgccggt gccgtcaggc gtcggataat cattgccgaa aacattaatg tgcggacggc
     2041 ggccgacggc cacctgactg agaaacggca tcagattatt cgggattccc cggggggact
     2101 cgccgatcag cccgctgaaa tgcgcaccca caggattgaa atagcgcaaa agaataatcc
     2161 gccacgcggg atccgcccgg tgactatcct gcagaatctg ttcgatcatc agcttggtgc
     2221 gggcgtacgg attggtcggg gcaggcggac agtcttccgt caccggaata atatttgctt
     2281 ccccgtaaac cgacgcggac gaactgaaaa taattttctt cacccggttg gccgccatga
     2341 cccggcacag cgacagcgcg cccataatat tattttcgta atagagcagg ggagcgtcaa
     2401 ccgattcacc caccgccttc agaccggcaa aatgaatgac cgaatcgatc ctgtgctttt
     2461 caaaaacctt gttgagcgcg ttggcgttgc gcaggtccag tttataaaaa ggcggttgct
     2521 ttcccgtgat gcgggcgatg catttgacaa ccgacgcctc gctgttggaa agattatcca
     2581 cgatgaccgg ccgatatccg gcctccatca acgacaccgc cgtatggctt ccgatgtatc
     2641 ctgtacctcc tgtgaccagc acttccgtca tcaacatgat ccttacagat ttgactttga
     2701 atgttttaga ttattttaaa gcaatgagtg tacatatcac attttaacaa gcaacataac
     2761 gtgtcttttt aaataaaata taaatatgtc atgtagagga gcatcctgga atgacagatt
     2821 cttttgtcat tcccgaatgg gcccgatgtt tgtcatgccg aggccggcat ccacccagtc
     2881 tgtcataccg gcgtaggccg gtatccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnngtc tgtcataccg gcgtaggccg gtatccagta ttattcattc ctggttcccg
     3061 gccttcgccg ggaagacgtc tggattccgg ttaggcccat gccggaatga cgcaactgtc
     3121 tgtcataccg gcgcaggccg gtatccacct ctgtctgtca taccggcgta ggccggtatc
     3181 cagtcacaca acatcaatat acaaatcgcg ccatgcagga ttatcttttt ctatcatttt
     3241 caacttccag gcgcgtttcc attcctttat atttttctcc ctgttaatgg cagaatccat
     3301 ggtgtcatga agctcaaacc agacaaggtc atggacacta taacgcttcg taaaaccatc
     3361 aaccaggtta ttcttatgtt cccatattct cttcacgaga tcagaggtca cacccgtata
     3421 aagtgttcca ttttttgcac ttgcgagaat ataaacagct ggttgtttct tcatttgtta
     3481 ttcctggttc ccggccttcg ccgggaagac gtttggattc cggctaaacc cacgccggaa
     3541 tgacagtggt acggtacacg cgggaatgac agtggtacgg agcatgaggg aaggacagcg
     3601 gtactttgaa ccatgaacca taaacccgtt ttttacttaa aacgtaaaac gtaaaactga
     3661 aaactatttt gcatgaacca taaactattt atgatccaag tcgactttga aaggcgcttt
     3721 atctttttta tcgattttac tctgaatata atccttcaac tttccggcgg tctcatccga
     3781 aagttgcgca aattccaccc ccgcctcgta atacttattt tcaataatca ttcttatcca
     3841 gacaattttc ccggtaacgg taatcgtttt atccgagccc gaattcatgc ggtaatcaat
     3901 tttgacccgc gtgccgaccg gaaggaacgc acttccgcgg attttcatac ccgacgacga
     3961 aatattttcg ctgtaattga agggaattat tctttcggaa cattgcgttt cttcggaaac
     4021 caccgacacg gaaacccaat ttaaaatttt caaccgctcc tcttttcttc tttcccgcat
     4081 tccaacgact ccctgtacga caaattggat aaaatgtaat gataaaggcc ctgcggaagg
     4141 ttggttcacg aacacaaaaa tggaggagat atcttctcct gatttccccc aggaaagggc
     4201 catccgcagg gcacaatccc ctgttccatt cacgatcaca tgaagaaaac ttttaaagca
     4261 atacggcaaa cagaataata tttcaacaag gcagacacat ggaattttga tgataaaacg
     4321 tgtacagaaa aatacacatt tatttgatgc ttctcacata agaccggaga ttgcttttgg
     4381 ttttcctgag gttgagcttc ttgcggatgt tattgcggtg cgtcgcgatc gtgttcaccg
     4441 agacgttaag aatggaggca atttcttttg taatttttcc tttgcggatc agatcgataa
     4501 tctgcatttc ctgcggcgtc atttttttat gatactcccg cagatccgtc ataaaaggcg
     4561 aaagcacgct gccgagattg gtttccagaa tgctgataaa ttttctgctg cgttcatcca
     4621 gatgcatatt cttgagcttg tcgatataag gcatcaccag atcgttgatg ttggtttgca
     4681 gcttgtcctc cagcatcttc ttgtcgttgt tactgtgatt gatcagcacg cgcagcgcga
     4741 tattggcatc ttccagactt tcggcaaagc gcttcagatc ctcttcggat ttctttttat
     4801 ccgtaatatc catcgccgtg cccatcatac agcgcttgcc tttataattg atgattctcg
     4861 aattaaacaa aaatgtttta aagctgccgt ccttgcagac aatctcgcct tcgccggaac
     4921 tttctcctat atcaaatccc gattttatct tttccacggt actgctgcgt ttatcgggcg
     4981 gtgcaagatt cagcgccgac atccggcgca aatcgtcgga tgtgtatcct gtgatgcggg
     5041 tataattatc attccagagc tggaacctga aatattcatc aatcacataa aacaaccccg
     5101 gcagattatt aataatcgcc ttgtttaaat tgtgttcatt atgcagagct tcttcaatat
     5161 tcttaatgtg cgtaatatcc tgcgccacca caatgacata atattcatcg ccgattttga
     5221 ccagatcgcc cgaatcaagg caagtgacca ttgaaccgtc ctttaaccgg tgctggactt
     5281 ccaggccccg gaaacaaccg tccttggcaa tacacgccag cgctttttcg cggatgtcct
     5341 gagaaaataa ctgcaaatcc tgactggtcc tgccgataat ctcttcccgt gaatagcccg
     5401 atccctggca caaaaaatca ttgacattgc agatcgttcc gtcttccggt ttaaaaaccg
     5461 caatcgcatt cggcgacaga ttgaagagct ggcgataaat ctcatcggcg gtgagcggca
     5521 ccaccggatt ttttcgaccc gcaggcgctg cccctttaac cgttttccct tttttgccgg
     5581 ttgttttggt tttctgcaac ttattcggtt tcccctgttt tgtttttccc ataacgtctt
     5641 ctcctaaccc cttgtgtttt caaatagtgt catccccgaa tgactccctg tttgtcatac
     5701 cggcgcaggc cggtatccag aaccctgtcc gtcatgccgg tgtagacc
//
LOCUS       DAONQZ010000105         5741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14581_length_5741_cov_13.231797, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000105 DAONQZ010000000
VERSION     DAONQZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14581_length_5741_cov_13.231797"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..992)
                     /gene="speB"
                     /locus_tag="PLR13_11120"
     CDS             complement(147..992)
                     /gene="speB"
                     /locus_tag="PLR13_11120"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQC19933.1"
                     /translation="MNFGGIYPAHSLHEATFVVVPVPYDLTSTYQPGSRRGPIAIIEA
                     STNMELYDEELKKETYLAGIHTTLPVDVDARGPKNMISAVRKKISKIVALNKIPVMLG
                     GEHSISLGAVQALKEKYPRLSVLQLDAHADLRDSYQGSPYSHASVARRISELCPLVQV
                     GIRSMSKEEGDFLPTSNVKSYSADFVGERKDWCEKISKDTKGDVFVTIDLDVFDPSIM
                     ASTGTPEPGGLYWRDVLRLLKSVSRTCKIRGFDVVELAPISGDIAPDFMAAKLIYRLM
                     GYITG"
     gene            complement(1032..1802)
                     /locus_tag="PLR13_11125"
     CDS             complement(1032..1802)
                     /locus_tag="PLR13_11125"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQC19934.1"
                     /translation="MKAIFISDAHLRKAGDERYKKLLKFFDAVQAGHVMTAADPGAKS
                     GRTLIDHLYIAGDLFDFWFCPKEKINPEFKPVIDKLNEMKNSGIGLHLCEGNHDFFMR
                     DYFADVLGLDVAEEFMEARLDHLNVLIAHGDTADSTNSNYLMLRKILRSNVFYNFQRF
                     IPASVRWSLAGLFSTASKELTTEDGNALVKKMEPFALNKFQEGFDAVILGHCHVPAIN
                     SYDVDGRKRTFVTLGDWITHYSFVYYENNNFFIHRYRG"
     gene            2027..3019
                     /locus_tag="PLR13_11130"
     CDS             2027..3019
                     /locus_tag="PLR13_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQC19935.1"
                     /translation="MTKKQNIIKRAKDVLKIEAQSILRLVDRVDSNFSQAVNLICKSK
                     GRVIITGIGKSGLIGRKIVATLTSTGTPALFMHPVEGLHGDLGIVTKEDILIAISNSG
                     ETRELMPVITSVRHIGAPIIAFTGGLSSTLARASDIVIDVSVEKEACPFNLVPTSSST
                     ATLAIGDALAIALLAEKQFNEKDFYKFHPGGNLGSRLREKVGEAMIVGKKIPKVPTGS
                     SVKKAIEEINRKNVGFVLITGKKNKLLGILTDGDVRRMISKGISFEGKSIDDVMTPDP
                     KTIDEKMSLAQAMEMMQKREITSFAVVNEKHELKGYVHLHDILGRGGSVSISLA"
     gene            4173..4700
                     /locus_tag="PLR13_11135"
     CDS             4173..4700
                     /locus_tag="PLR13_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQC19936.1"
                     /translation="MALYEFDGKRPLVPDDSFVHPQAALIGDIEIGHECLIAPGVSIR
                     ADFGPVIIGDRSNVQDNSVIHVFPGSKVIIEQDVIVAHGVILHDVHIKSRCVIGIGAV
                     LLFGAVCEEDVFVSVGAVVSKGMQIPAGKVVAGNPAKITRDVSDDQKLMAKEGINYYR
                     ELCRKYIATMQQVSD"
     gene            complement(4841..>5741)
                     /locus_tag="PLR13_11140"
     CDS             complement(4841..>5741)
                     /locus_tag="PLR13_11140"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQC19937.1"
                     /translation="YENALVAIEEKVPVLNISLGKGGELIKKVHAYGGKVISTVTTVE
                     HALAAQKSGADALQVTGFEAAAHGSQVTTLVLVPTVVDAVKIPVVAIGGIADGRGMAA
                     AFALGAEGVGMGTRLSITKESPVHQLAMQKQLELGAEDTIYSNRFDALYCRVLKTPSA
                     EKAVKQGRNLAKGLAASFDIAKSLDLSWMKLATSILGGSKKDKSGKPKEADTAPKQKK
                     GEISLFQKMMKGPQTGMNLAHMAQAYVAIKKATENGDLKDGVYLSGQVQGIIHDIPTV
                     AEVINRTVEEFTRIQKDMAGEAV"
BASE COUNT         1423 a         1419 c         1338 g         1561 t
ORIGIN      
        1 ctgacctttg ggttatgagc ccaacgagct accggactgc tccaccccgc gatatttgaa
       61 aaacgttgac tttacaccat tatctccact gtttgtcaag gagtaatttt caaaatcctt
      121 gatcgatatc aacggattga ataatcttac ccggttatgt agcccataag gcggtaaatc
      181 agtttggcgg ccatgaaatc aggcgcgata tcgccggaaa tcggggccag ttccacgaca
      241 tcgaagccgc ggatcttgca ggtgcgtgaa acggatttaa gcaggcgcag cacatccctc
      301 cagtacagac ctcccggttc cggtgtcccg gtggacgcca tgatggaagg gtcgaaaaca
      361 tccagatcga tggtgacaaa aacatcgccc ttggtgtcct tgctgatttt ctcacaccag
      421 tcttttcttt ccccgacaaa gtccgcgctg taggatttca cgttgctcgt cggcaaaaaa
      481 tcgccttcct ccttactcat gctccggatg cccacctgaa caagcgggca aagctcagaa
      541 attcttcggg cgacggaagc atgactgtag gggcttccct ggtagctgtc ccttaaatcg
      601 gcatgagcgt ccagttgtag aacggaaagt ctgggatatt tttctttcag cgcctgcacc
      661 gctcccaggc tgatgctgtg ttcgccgccc agcatgacgg gtattttatt cagagcgaca
      721 attttggaaa tctttttccg cacggctgag atcatgtttt taggccctcg ggcgtcaaca
      781 tcgaccggaa gcgtcgtgtg aattccggcc agatacgttt ctttcttcaa ttcctcatcg
      841 tagagctcca tgttggtgga cgcctcgatg atggctatcg gtccgcggcg gcttcccggc
      901 tgatatgtcg acgtcagatc atagggaacc ggcaccacga caaatgttgc ttcatgcagt
      961 gaatgcgcgg gataaattcc tccaaaattc attatggtca tcctttctta aattaaattc
     1021 ggtttgggtc atcagccccg gtaacgatga ataaaaaaat tattattctc ataatagaca
     1081 aaggaataat gcgttatcca gtcgcccaga gttacaaacg ttcttttccg gccgtccaca
     1141 tcataactat tgatggccgg cacatgacag tggcctagaa taacggcatc aaaaccttcc
     1201 tgaaatttgt tcagggcgaa cggctccatt tttttaacta gagcgttgcc gtcttccgtg
     1261 gtgagctcct tgctggccgt agaaaacagc cccgccaggg accagcgaac ggacgccggg
     1321 ataaaacgct gaaagttgta aaaaacgtta ctgcgcagaa ttttacgcaa catcaggtaa
     1381 ttggaattgg tgctgtctgc agtgtcgccg tgggcgatca acacgttcag atgatccagc
     1441 cgggcttcca tgaattcctc ggcgacatcc aatcccagca catcggcgaa atagtctctc
     1501 ataaaaaaat cgtgattgcc ttcgcataag tgaaggccga tgccggaatt tttcatctcg
     1561 tttaatttgt caatcacggg tttgaactcc ggattgattt tctctttcgg acaaaaccag
     1621 aaatcaaaca agtctccggc aatataaaga tgatcaatca acgttctccc ggattttgcg
     1681 cccggatcgg ctgccgtcat cacatggcct gcctgaacgg catcaaaaaa ttttagcagt
     1741 ttcttatatc gttcatcgcc ggcttttcgt aaatgcgcat cggaaatgaa aatggctttc
     1801 ataggttaaa tccgcgtgtt taaagtgaat cttgtttagc agatacaggt cttcaggtca
     1861 ataaacataa aaaagaacgc aatatcggca catttccggc ggcggacgaa tgatttttcc
     1921 cgaacccggc tgaattggac cttgttttgt cgggattttt ttgctagtga aagctcgtca
     1981 tgaccgaaac gtgatcagaa ttcaatgttt gcagagaagg cgaaaaatga caaaaaaaca
     2041 aaacatcatc aaacgggcaa aagacgttct gaaaattgaa gcgcagagca ttttgcgatt
     2101 agtggataga gtggatagta acttttctca agccgttaac ttgatatgca aatccaaggg
     2161 aagggtgatc atcaccggca tcggcaagtc cggtctgatc ggacgtaaaa ttgtagcaac
     2221 gctcaccagc acgggcacac cggctctctt tatgcatccg gttgagggcc tgcacggcga
     2281 tctgggcatc gttaccaaag aagatatcct cattgccatc tccaacagcg gcgaaaccag
     2341 agaactgatg cccgtcatca cttccgttcg ccatatcggc gctcccatta tcgcttttac
     2401 cggcggcctg tcttcaacgc tggctcgggc aagcgacatc gttattgatg tgtccgtgga
     2461 aaaggaggcc tgtccgttca atcttgttcc cacgtccagt tccaccgcca ccctggcgat
     2521 tggcgatgcc ctggcgattg cactgctcgc ggaaaagcag ttcaatgaaa aagatttcta
     2581 taaatttcat cccggaggaa atcttggttc gagattgcgc gaaaaggttg gcgaagccat
     2641 gatcgtggga aagaaaattc ccaaggttcc gacaggaagc tccgtgaaga aggcgataga
     2701 ggaaatcaac cgcaaaaatg tcggatttgt tttgattacc ggcaaaaaaa ataaattact
     2761 gggaattctc accgacggcg atgtgcgccg tatgatcagc aaaggcatat cgtttgaagg
     2821 aaaatccatc gacgacgtca tgacccccga cccgaaaacc atagatgaaa aaatgtctct
     2881 ggctcaggcc atggaaatga tgcagaagcg ggaaattacc tcctttgccg tggtcaatga
     2941 aaaacacgaa ctcaagggat acgtccactt gcatgacatc ctcggccgcg gcggctccgt
     3001 cagcatctct cttgcgtgat tttgcgcgtg ctcaggaaat actgatcctt caagacctga
     3061 cacaatatgt gataaaaatg gttagtctgc aagagccaag caaagatttg acctgttccc
     3121 ccagttgtca tccccgaatg ggcttatcgg ggatccagta ttgttcatac cattaattag
     3181 accgtcccca ttattcccat cgcttatcct tgggttacca aacctgtttc cattatttta
     3241 caattttaag ggcgtcccgc tttcccagta tattttcaga cacaaatata aacaacttta
     3301 gatcagcgaa gacaaaccgg aatatgagat cccatcatgt tctggtatta tacagcacac
     3361 tttttgacca ataggatctt gcactttttc tcccaacttg aattgtctat tgaaatacaa
     3421 cgcagacttt ttgattcccc atccacgtta aaggtgttta ttatcctacg tcgctacgct
     3481 cccaggataa ttcgactaac tcttcaaact tcatcccgat ttgtcgggat tcgttttacc
     3541 acggcacttt gtgcctggta tagttcgcct tacgcttcat acttcattcc gactcgtcgg
     3601 gattcgtctt cagcttggct cactatcaga gagtctcatt aaaactgttt ttgtgaaaac
     3661 ttgtgaagcc cccgtacgca acgcttcggg gatatcccta ccacagaggc ggttgggatt
     3721 gttcgtccca caaatttcag tacgttccac ttctgaaact tgggactcac taattcgact
     3781 aactcttcaa atcccacggt tttttgagcc tgggatagtt cgacttgcaa atattactgc
     3841 actacgattg taatatttga gtctcactac tttgtcccga tacatcggga ctcgttttac
     3901 cacggcactt tgtgcctggt atagttcgcc ttacgcttca gacttcattc cgactcgtcg
     3961 ggattcgtct tcagcttggc tcactatcag agggtctcat tatatttttg attcgataag
     4021 actgatattc tataaatgaa ttgcccgtac ttgtcaataa tgttttctta ataatttcaa
     4081 gaggtaagaa tatcatcctg cttacggtta actaaatatg tggcaccatg cttgtatttt
     4141 ttgtatatta aaaaaacagg agaaataatt gtatggcgct ttatgaattt gacggtaaaa
     4201 gaccactggt gccggatgat tcttttgttc atccgcaggc tgccttgatt ggggatatcg
     4261 aaatcggcca tgaatgtctg attgcgcccg gtgtttccat ccgcgccgat ttcggtccgg
     4321 tcatcatcgg agatcgttcc aatgttcagg acaacagcgt cattcatgtc tttcccggat
     4381 caaaggtcat tattgagcag gatgtcattg tggctcatgg cgtgattctt catgatgtgc
     4441 acatcaaatc ccgatgtgta atcggcattg gcgctgtttt gcttttcggc gcggtctgcg
     4501 aagaagatgt ttttgtttcc gtgggtgcgg tcgtgtccaa aggcatgcag attcccgccg
     4561 gcaaagtggt tgcgggcaat ccggcgaaaa tcacccgcga tgtttctgat gatcagaagt
     4621 tgatggcgaa agaggggatt aactattatc gggagctttg cagaaaatac attgcaacga
     4681 tgcaacaagt cagtgattaa ccgtccaaca atagtgacga cattgggaag atagccatca
     4741 cacttgtcac accggcggag gccggggtcc agtttaaata attctggatt ccggctcaga
     4801 ggccggaatc cagaattata gatatctaca agataatgtt ctaaacggct tcccccgcca
     4861 tatctttttg aattctggta aattcttcaa ccgttcgatt gatgacttcg gccacggtcg
     4921 gaatgtcgtg gataatgccc tgtacctgac cggaaagata cacgccgtct ttgagatcgc
     4981 cgttttccgt cgctttcttg atggcgacat aggcctgcgc catatgtgcc agattcattc
     5041 cggtttgcgg acctttcatc atcttctgga ataacgatat ttcccccttt ttttgtttcg
     5101 gtgcggtatc ggcttctttg ggttttcccg atttatcttt tttcgagccg cctaaaatcg
     5161 atgtcgccag cttcatccag gataaatcca gcgatttggc gatgtcgaat gatgcggcaa
     5221 gtcccttggc cagattacgc ccctgcttga cggctttttc ggcggaggga gttttcagaa
     5281 cccggcagta aagcgcgtcg aaccggttgg agtagatggt gtcttccgct cccagttcga
     5341 gttgcttctg catggcaagt tgatgaacgg gactttcttt tgtgatcgag agcctagttc
     5401 ccatacccac gccttcggcg ccgagcgcga aagcggcggc catgcctctg ccgtccgcga
     5461 tgccgccaat agcgaccacg ggaattttaa ccgcatcaac aaccgtcgga accagaacca
     5521 gcgtggtcac ctggctgccg tgcgccgcgg cttcaaagcc cgtcacctga agagcgtccg
     5581 ctccggattt ctgggcggca agcgcgtgtt cgaccgtggt gaccgtcgat atgactttcc
     5641 cgccgtaggc gtgtactttt ttgattaatt ctccgccctt gcccaaagat atgttgagaa
     5701 ccgggacttt ttcctcgatg gcgaccagcg cgttttcata t
//
LOCUS       DAONQZ010000106         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14601_length_5736_cov_19.049815, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000106 DAONQZ010000000
VERSION     DAONQZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14601_length_5736_cov_19.049815"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..524
                     /locus_tag="PLR13_11145"
     CDS             75..524
                     /locus_tag="PLR13_11145"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HQC19938.1"
                     /translation="MKIYFAGAIRAGRQDAEIYKAMIEVLMSFGDVLTEHVGNPALSE
                     KGNDGPHDRFIHDRDMAWLESCDCVVAEVTKPSLGVGYELGWATAMKRPVLCLYRRQS
                     KKVLSAMIAGSPGIRTVAYSSMDEARSIVEKFIKKAVQKISTSSGRE"
     gene            525..2351
                     /locus_tag="PLR13_11150"
     CDS             525..2351
                     /locus_tag="PLR13_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQC19939.1"
                     /translation="MVTSLELLAPAGDAAIGIAAIDHGADAVYIGAPKFSARAAAGVQ
                     LDDIARLIEHAHFYRAKVYVALNTILTDSELAESLDLIRAIDALGADGLIIQDVGLLE
                     LDLPPIPLIASTQMHNSTPEKVRFLEAVGFQRVILARELSLSEIAGIRRQAPKIELET
                     FVHGALCVSYSGQCYMSHAVAGRSGNRGVCAQPCRSHYTLIDGDGNTIMKNKYLLSLK
                     DLNLMSRVPDLIAAGSTCFKIEGRYKGMDYVKNVTAAYRQVIDRFINEHAGYRRSSSG
                     ASELAFTPDVGRTFNRGYSQHFVGGKREKIASLDTQKSIGQYLGKITAAGKDYFQIDR
                     DDLQNGDGLCFFTRQNNLAGSRVNRVEKGKVYPNSMKGLEAGLSLYRNLDTAFAKALK
                     KTSAKRLIAVEMDFSQADTLIRLAAKDEDGNQVEAVKDVLFEPSRNPAGARGQIEKQL
                     TSAGNTPFRISRLTIEPRAPGYLSASVLNGIRRDVLENLARVRRETYVRGDRPFCPND
                     VAYPEKHLDFHANVFNAAARRFYERHGATVTEPAFETLSDFAGKTLMTTRYCIRYQLD
                     LCPRLQQPESSVREPLYLRDAHHTYRLEFDCKHCRMSVILEN"
     gene            complement(2449..3012)
                     /locus_tag="PLR13_11155"
     CDS             complement(2449..3012)
                     /locus_tag="PLR13_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19940.1"
                     /translation="MNAKGIAYIVTKSNMIAAFGEERWSAFMEKLAGKDSYFSTVILT
                     ITPMPVEKLIICFDEMCAEFFNNDKMQYTYFGKAGAKAVLSPTGPYRSFMQMTNDMKQ
                     FIETVLPKVWSSYFDSGVAVAKFENNVAHIKITGLEIKYTYFEYLVMGYFQQAIKMFG
                     KKSSAKRIRSLAAGDKDMYFQYALKDS"
     gene            complement(3405..4235)
                     /locus_tag="PLR13_11160"
     CDS             complement(3405..4235)
                     /locus_tag="PLR13_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQC19941.1"
                     /translation="MALFRERDGRRLHVKSRLMGESLVSKKFMEDLKVAPQERLFRDV
                     NIVKIGGQSICDRGVKALPAIVKEVVANKKKHKILLTTGGGTRSRHIYSIGLELGMPT
                     GIIAKFGSSISEQNALLVSTLLAPWGGIKIGHDEITKLYNYFGQDCIPVMHGMPPYDY
                     FALPVAKSRIPIHRTDVGTLVIADLIGARSCIFVKDERGLYTDDPKKNSKAKFIPEIS
                     VQDLLKKNLDDLIIERPCLDILLNSEVIDRIQIINGLEKGNLTKALNGKHVGTIIYKS
                     "
     gene            complement(4257..4484)
                     /locus_tag="PLR13_11165"
     CDS             complement(4257..4484)
                     /locus_tag="PLR13_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19942.1"
                     /translation="MMAGKIFYRERIKVGEKEKKPRFKLVAVADVNITFYADHLKKRE
                     LEEIAASVGAELVLIKAPKDGTVKEEEIEVK"
     gene            complement(4494..5237)
                     /locus_tag="PLR13_11170"
     CDS             complement(4494..5237)
                     /locus_tag="PLR13_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19943.1"
                     /translation="MMAKMRVYIGFDDTDIVEADYGTGKVARRFEKVLPAQCKIWGVV
                     RQQLLVDPAIPYTSHNSSACVVVDCPDSSYVDALKSAAIKHIEAMSLPGSDPGLSVVV
                     EGDLALPALEQFGRQCTARVVTLKDAVKAAGSAHLSGHGGTNGGMIGAAAAVGLTASG
                     WSGRFIEYDGLRDFPDEVIVAALMQKNIRVISLDRDACCPRPEDMVNTKGWLRPRLWG
                     HDVVLPVKPAGAGLWESLGEKRNPDKKKK"
     gene            5433..>5736
                     /locus_tag="PLR13_11175"
     CDS             5433..>5736
                     /locus_tag="PLR13_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQC19944.1"
                     /translation="MKTDEYKKIIMFAVDNEIEAYEFYKAVADKTKDGGLKKIFTELA
                     QEEAGHRKLLEGLASGKGQMHFDESKDYKVAESINKPKLSMSMKPADAIALAVKNEQ"
BASE COUNT         1429 a         1450 c         1437 g         1420 t
ORIGIN      
        1 aaatgtgttg aatgcatccg accatcagtg gtatcaatag caaaagtgtt tcatcgcctg
       61 ggcgtaggga gtttatgaaa atttattttg ccggagcgat tcgcgccgga aggcaggatg
      121 ccgaaatcta caaagcaatg attgaggttt taatgtcatt tggcgacgtc ctgaccgaac
      181 acgtcggcaa tcccgctctg tcggaaaaag gcaatgacgg accgcatgac cggtttattc
      241 atgaccgcga catggcctgg ctggaatcct gcgattgcgt ggtggccgag gtgaccaaac
      301 cttctctggg tgtgggctat gaactgggat gggccacggc aatgaaaagg cctgttttgt
      361 gcctgtatcg acgccaatcg aagaaagttt tgtccgccat gattgcaggc agtcccggca
      421 tccggacggt tgcctattcc tccatggacg aagcgagaag tattgtggag aaatttatca
      481 aaaaggccgt tcaaaaaata tcaacttctt ccgggagaga atagatggtc acatcactgg
      541 aattgctggc gccggcaggc gatgccgcta tcggaatagc agccatcgat catggcgcgg
      601 atgccgtgta tatcggcgcg ccgaaattca gcgcccgggc cgcagccggt gtgcagctcg
      661 acgatatcgc ccgtctgatt gaacacgccc acttctatcg ggcgaaagtt tatgtggcgc
      721 tcaataccat tcttaccgat tcggaactgg ccgagtcgct ggatctgatc agggccatcg
      781 atgcgctggg cgccgacggg ctgattattc aggatgtcgg tttgctggag ctggacttgc
      841 cgcccattcc gctgattgcc agcactcaga tgcacaacag tacgccggag aaggtccggt
      901 ttctggaggc cgtgggattt cagcgggtta ttctggcccg ggaactttct ctttccgaga
      961 ttgccgggat tcgccgtcag gccccgaaga tagaactgga aacgtttgtg catggcgcgt
     1021 tatgcgtttc ctacagcgga caatgttata tgagtcatgc cgttgcgggg cgcagcggca
     1081 atcgcggcgt ttgcgcgcag ccctgccggt cccattacac gctgatcgac ggtgacggca
     1141 acaccataat gaaaaataaa tatctccttt cgctcaagga tttgaacctg atgagccggg
     1201 tgccggattt gattgccgca ggctcgacct gttttaaaat cgaggggcga tacaagggga
     1261 tggactatgt caaaaacgtc acggccgctt accggcaggt gatcgatcga ttcatcaacg
     1321 agcacgccgg ttaccggcgt agcagttccg gcgcaagcga gcttgcattc acgcccgatg
     1381 tcggcagaac gttcaaccgc ggttacagcc agcactttgt cggcgggaaa agagaaaaga
     1441 tcgcttcgct cgatacgcaa aaatccatcg gccagtatct ggggaaaatc accgcggcgg
     1501 gaaaagatta ttttcaaatc gaccgtgatg atctgcaaaa cggggacgga ctttgctttt
     1561 tcaccaggca aaacaatctg gctggctcca gagtgaaccg ggtagagaag gggaaggttt
     1621 atcccaacag catgaaaggc ctggaggccg gactgtcgct ttatcgcaat ctcgacaccg
     1681 cgtttgccaa agcgttaaag aaaacatcgg ccaaacgatt gattgcggtg gagatggatt
     1741 tcagccaggc cgatacgttg atccggctgg cggcaaaaga tgaagacggc aatcaggtgg
     1801 aagccgtaaa ggatgttttg tttgaaccgt cgcgcaatcc ggccggagca aggggccaaa
     1861 tcgaaaaaca actgaccagc gccggcaaca cgccgtttag gatatcgcgc ctgaccattg
     1921 aaccccgcgc gccgggctat ctttccgcaa gcgtcttgaa cggcatccgg cgcgatgttc
     1981 tggaaaatct ggcgcgcgtg agacgtgaaa cctatgttcg cggggaccgg ccgttttgtc
     2041 ccaatgacgt tgcttatccg gaaaaacacc tcgattttca cgccaatgtt ttcaatgcgg
     2101 cggccagacg gttctatgaa cggcacggcg caaccgttac cgaaccggcc tttgaaacgc
     2161 tttccgattt tgccggtaaa accctcatga ccacgcgcta ctgtatccgg tatcagttgg
     2221 atctgtgccc ccggctgcag caaccggagt cctccgtcag agaaccgttg tatcttcggg
     2281 atgctcatca tacctatcgt cttgaattcg actgcaagca ctgccggatg tcggtaattc
     2341 tggaaaactg acggaagcgc ggaaaccgga ttaaggcgcg cgggtatttc tatccgttaa
     2401 gcggggatca attacagagt attaaaaata ctgcaccggc gcggatggtc aggaatcttt
     2461 aagcgcgtac tgaaaataca tatctttatc ccctgcggca aggctcctga tcctttttgc
     2521 agacgatttc ttcccgaaca tttttatggc ctgctggaag taacccatca cgaggtattc
     2581 aaaatacgta tatttgattt caagaccggt aatcttgata tgagcaacat tgttttcaaa
     2641 ctttgcaacg gcgacgccgc tgtcaaaata agaagaccag accttgggca gcaccgtctc
     2701 gataaattgt ttcatgtcat ttgtcatctg cataaacgac ctgtagggtc ccgtcggcga
     2761 cagaaccgct ttggcgcccg ccttgccgaa atatgtatac tgcattttgt cgttgttgaa
     2821 aaattcggca cacatttcat caaaacaaat gattaatttt tccaccggca tgggagtaat
     2881 ggtgaggatg accgtgctga agtaactgtc cttcccggcg agtttttcca taaaggccga
     2941 ccatctttcc tcgccaaatg ctgcaatcat atttgatttt gttacaatgt aagcaatgcc
     3001 tttagcgttc atgtctgaaa tcctcccccg gattgttctg tcatcaaggt gtttccaacg
     3061 tctgttatgt gcggcatggt gcaatctaac ataaaatggc cggatagaaa attatgaatc
     3121 tttaaaatga gcgatcggaa gcgattcgca tcactgccgg atgtccggca aaacgtatca
     3181 cggcgatcaa tctacattgc tccggaaaat taatcaatcc ctctaaagag ggatatgtgt
     3241 catttttaca taattgctgt tccagtaatg gccgacttta ctgtttgtct catcggcagc
     3301 aacaaaaaga ttttatggcg gataatcagg gggaaatctg attgcaaaaa aagaaggctt
     3361 cgtaataccg aagccttctt ttttgtgatg atgcggcctg acttctatga cttgtaaatg
     3421 attgtgccga cgtgtttgcc gttaagggcc ttggtcaggt tgcccttttc cagaccattg
     3481 ataatctgaa tcctgtcgat gacttcactg ttgagcagga tgtccaggca cggtctttcg
     3541 ataatcagat catcaagatt ttttttcaga agatcctgga cgctgatttc gggaataaat
     3601 ttcgccttgc tgtttttctt cggatcgtcc gtgtagagtc cccgctcgtc cttgacaaaa
     3661 atgcaactgc gggcgccgat taaatcggca atcaccagcg tgcccacatc ggtgcggtga
     3721 atgggaatgc gggattttgc cacgggcagg gcaaagtaat cgtaaggcgg catgccgtgc
     3781 atgacgggaa tgcagtcctg gccgaaataa ttgtatagtt ttgtaatttc atcatggccg
     3841 atcttgatgc cgccccaggg agccagcagt gtggaaacca gcagggcgtt ctgttcggaa
     3901 atggaactgc cgaacttggc gatgatgccg gtgggcatcc ccaactccaa accgatggaa
     3961 taaatgtggc ggctgcgcgt gccgccgcct gtcgtcagca gaatcttgtg tttcttcttg
     4021 ttggcgacaa cttccttgac gatggccggc aaggccttca ccccgcgatc gcaaatggat
     4081 tgcccgccga tcttgacgat attcacatcc cggaaaagcc tttcctgagg agccaccttc
     4141 aggtcttcca taaatttttt gctcaccagg ctctcgccca taagcctgct tttcacatgc
     4201 agcctgcgtc cgtctctttc tctgaacagt gccataaatt caatcctcaa gaatgtttac
     4261 ttgacttcaa tttcttcttc cttgactgtt ccatctttcg gcgccttgat taaaacaagt
     4321 tccgcgccca ccgaagcggc aatttcctcg agctctcttt tcttcaggtg gtcggcgtaa
     4381 aaagtgatat tcacatcggc aaccgccacc agtttgaaac gcggcttctt ctctttttcg
     4441 ccaaccttga ttctttcgcg ataaaatatt ttccctgcca tcattctacc tccctatttt
     4501 ttctttttgt cagggtttcg tttctctccc aggctttccc acaggcccgc gcctgccggt
     4561 ttaaccggaa gcacaacgtc atgcccccag agcctgggtc ttaaccagcc ttttgtattc
     4621 accatgtctt ccggacgggg gcaacacgca tcccggtcca gagaaatcac ccgaatattt
     4681 ttttgcatca gcgccgcaac aattacctcg tcgggaaaat ctctcaaccc atcatactcg
     4741 ataaaacgtc cgctccatcc cgaagcggtg agaccgacag ccgccgcggc tccgatcatg
     4801 ccgccgttgg ttccgccgtg accggaaaga tgagcgctgc ccgccgcctt tacggcgtcc
     4861 ttcagggtaa cgactcttgc tgtgcactgc cttccaaatt gctccagcgc aggcagcgcc
     4921 agatcgcctt cgacaaccac ggaaaggccg ggatcgctgc cgggaagcga catcgcttcg
     4981 atgtgtttaa tggccgcgct ttttaaagca tccacatacg atgaatccgg gcagtccaca
     5041 accacacagg ctgaactgtt gtgcgacgta taaggaatgg ccggatctac aagcaactgc
     5101 tggcggacaa caccccagat tttgcattga gcaggcagga ctttttcaaa acggcgggcg
     5161 acctttcccg ttccgtaatc cgcctccaca atgtccgtat cgtcaaaacc aatataaacg
     5221 cgcatctttg ccatcatgtt aaaaaaaaca tccttaaccg attatagagt caccgtttta
     5281 gcctgtcaat gaattaaaaa tgtaaacatc gaaattctca aatgatttgt gataatatat
     5341 actttgacgc aatccattat ttcctgtata ttactaaaac gattcgaaat gaattaaatg
     5401 aagatcatag atgattgcca aggaggattg aaatgaaaac ggatgaatac aaaaaaataa
     5461 tcatgtttgc agttgacaat gaaattgaag cctacgagtt ttacaaggcg gttgccgata
     5521 aaacaaaaga tggcggtttg aagaaaatct ttacggaact ggcgcaggaa gaagccggtc
     5581 atcgcaaact tctggaaggc ttggcatccg gaaagggcca gatgcatttt gacgaatcca
     5641 aagattacaa agtggccgaa tcaatcaata agcccaaact gagtatgagc atgaaaccgg
     5701 cggatgccat cgcgctggcg gtcaagaatg aacagg
//
LOCUS       DAONQZ010000107         5635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_14906_length_5635_cov_17.987097, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000107 DAONQZ010000000
VERSION     DAONQZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14906_length_5635_cov_17.987097"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLR13_11180"
     CDS             complement(<1..480)
                     /locus_tag="PLR13_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HQC19945.1"
                     /translation="MSKTLGKIFTILAVVILAGVFNANAAEEARDGRFIAYDDGTVWD
                     SQSNLLWASHDNGSNINWSDAEEYCKTYDGSGYKGWRLPTKDELLTLYDRSIFGNNGY
                     RLTKLITLSGSYPWTSDVQGSEALVVGFDKDRQMWFPESVNHKSRNRVLPVRFGKEGK
                     "
     gene            complement(699..>1424)
                     /locus_tag="PLR13_11185"
     CDS             complement(699..>1424)
                     /locus_tag="PLR13_11185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC19946.1"
                     /translation="ENWIACNSGWMWLTQKTRWDVQGAENLNHEGWYMVVSNHQSWVD
                     ILVMQHLLNRKIPLLKFFIKRELIKVPLMGFAWWALDFPFLYRHSSEYLKQHPEQAGK
                     DFEATRKACEKFATIPTSVMNFLEGTRFTPAKHDKQKSEYKYLLRPKSGGLALALNVL
                     GEKFSSMLDITIVYPDGIPGFGDFLCGRVKRITVRMRTIEIPKTLLQGDYENDQAFRD
                     SMHKWVQKLWREKDKQIQALMKK"
     assembly_gap    1425..1524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1525..3039
                     /locus_tag="PLR13_11190"
     CDS             <1525..3039
                     /locus_tag="PLR13_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQC19947.1"
                     /translation="ASEGPLPQTRFVLKKALGLRLPIILVINKIDRSDARIEEVISET
                     YDLFIDLGAEEHQIEFPILYTNAKAGISHEKIGDDSADLQPLLQTIIEYIPPPQGDDE
                     AITQFLVTNLDYDPYVGQIAVGRLQSGKLEMNTNYSLCTEYKIIPGVKLTALYTFFGL
                     AKKPAQSVESGDIIALSGVENVKIGDTISSLAEPRPLPRLLVDEPTVSMMFYVNDSPF
                     GGKEGKYLTSRHLLERLEKEQLRNVAIKIKKLERTDAFEVCGRGELQMAVLIETMRRE
                     GYELMVSRPQVITRQVNGKTCEPVERLFLDIPEEFVGKITEKLSIRKGRMESLVNKGS
                     GRVSMEFLIPSRGLIGFRNQFLTDTKGGGVMNSLLEDYAPWFGSIPQRTTGVLVADRS
                     GRVTPYASYAMEDRGEMIVEIGTDVYAGMIVGERNRSSDLNVNIIKEKKLTNMRASTS
                     DATVILRPPRILSLDQAIEFIAEDELVEVTPKSVRLRKMELDATKRQMKSRREE"
     gene            3064..4041
                     /locus_tag="PLR13_11195"
     CDS             3064..4041
                     /locus_tag="PLR13_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQC19948.1"
                     /translation="MISKIEKDFYQITLRMPYRLRHVNAYLFIYEGKLALFDTGLNTP
                     QAFETLGNDLSGIGFGIEDIQHIYLTHVHSDHCSMAGVLQKRSGAQIHLAQAAFEEYR
                     HFREAGPTIKQLRIYYARQGMSQEQIGLVIEEYENIRGIIADFDATDFLRDKDVREFG
                     NWKIEIISTPGHAAGHVCFFCREMKFLLSGDHVLPYIAPVLSPDIFDEAFRPLATYLD
                     SLNALENLSVSAVYPGHGLAFTDLKERLTDIREHHSGRKKAVLEHVNKQPKATCEISR
                     EMVSPNLADFDKFLALNETIAYLKELTAEGAIKEDTVNDVLVYVRPSGY"
     gene            4104..>5635
                     /locus_tag="PLR13_11200"
     CDS             4104..>5635
                     /locus_tag="PLR13_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQC19949.1"
                     /translation="MNKQLQKEIERRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGA
                     VKARKASKHALSDWMAIEKDRGISVTSSVMKFEYADCEINLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDGAKGVETQTQKLMEVCRLRNTPIITFINKLDRDCLPPLEILSDIEDK
                     LQIECSPLSWPVGMGKRFKGVYNMFEKKLHLFTPGKARRSEEGITINDLSDPLLDELL
                     ESQADELREEVALLEGAANPFELKEYLKASQTPVFFGSAINNFGVKELLDAFVEIAPP
                     PAARPTTTRAVEPEEEDFSGFVFKIQANMDPAHRDRIAFLRICSGQFQRGMKVIHHRI
                     GKEVSLANATIFMAQDRTNIEEAYPGDIIGLHNHGTIKIGDTFTQKEPLKFTGIPHFA
                     PEHFCRVRLKDPLRMKHLQKGLMQLSEEGAIQVFRPLWGSDNILGAVGALQFDVTMSR
                     LKDEYSVYADYEKTDYAAARWVTCDDAKIMEDFQKKNQTNLALDTEGNLVYLALSEWR
                     LG"
BASE COUNT         1398 a         1401 c         1395 g         1341 t
ORIGIN      
        1 cttgccttcc ttgccgaaac gcacgggcag taccctgttt ctgcttttgt ggttgacgga
       61 ctcgggaaac cacatttgac ggtctttgtc gaaaccgacc accagggctt ccgaaccctg
      121 aacgtcggat gtccatggat acgagccgct cagggtaatc agttttgtca gacgataccc
      181 gttattgccg aagatagacc ggtcgtataa ggtcagcaat tcatcttttg tcggcaggcg
      241 ccatcccttg taaccgctgc cgtcatacgt cttgcaatat tcctccgcat ccgaccagtt
      301 tatattgctt ccgttatcgt gggaggccca taacaaattg ctctgcgaat cccatactgt
      361 gccgtcatcg taggcgatga aacggccgtc gcgcgcttct tctgcagcat tggcgttaaa
      421 aacaccggcc agaataacga ccgccagaat agtaaaaatt ttccctaatg ttttactcat
      481 tagccgttaa ctcctcctct gttaatttgt tttgttctga gacgagttat tttttttgtg
      541 atgtcgcaat aatttaattg cctgaaatat tgcataattt tcagaattcg tcaattacgg
      601 aaacagcacc gcttttttct tcagttcacg aaagaaaatc ttctccggca ggccatccgg
      661 ttattttcat gccggcaacg tcattcctca cgggggcttc attttttcat cagcgcttgt
      721 atctgcttgt ccttttcacg ccagagtttc tgcacccatt tgtgcatgga atcacgaaac
      781 gcctgatcat tttcataatc gccctgcaaa agggtcttcg ggatttctat ggttctcatg
      841 cgcacggtga tgcgtttgac cctgccgcag aggaaatctc cgaacccggg aatgccgtcg
      901 ggataaacga tggtgatatc cagcatggaa ctgaattttt cgcccagaac gtttaaggcc
      961 agcgccagtc cgccggattt gggacgcaag agatatttgt attcgctttt ctgtttgtca
     1021 tgctttgccg gggtaaaacg tgttccttcc aggaaattca tcacactggt gggaatagtg
     1081 gcgaattttt cgcaggcttt gcgggttgct tcaaaatcct tgcccgcctg ttcgggatgc
     1141 tgtttcagat attcggagct gtggcgatac aggaaaggga agtccagcgc ccaccaggcg
     1201 aaacccatca aaggcacctt gatcagttcc cgcttgataa agaatttcaa aagaggaatt
     1261 ttacgattca acaaatgctg catcaccaga atatcgaccc acgattgatg gttgctgacc
     1321 accatgtacc agccttcatg attcaggttc tcggctccct gaacatccca ccgtgttttc
     1381 tgcgtcagcc acatccagcc gctgttgcag gcaatccagt tttcnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnngccagc gaaggtcccc tgccgcaaac ccgttttgtt
     1561 ttgaaaaaag cgctcggcct gcgcctgccc attattctgg tcattaataa aatcgaccgc
     1621 tctgatgcgc gcattgagga agtcatcagc gaaacctatg atctgttcat cgatctgggc
     1681 gctgaagagc atcagatcga gtttcctatt ctctatacca acgccaaggc cggtatcagc
     1741 catgaaaaaa taggcgatga cagtgccgat ttgcagccgc tgctacagac catcattgaa
     1801 tacatacctc cgccgcaggg agacgatgaa gctattacgc agtttctggt taccaatctg
     1861 gattatgatc cttatgtcgg acaaattgcc gtcggccgtc tacagagcgg caagttagag
     1921 atgaatacca attacagtct ttgcactgaa tataaaataa ttcccggcgt caagctgact
     1981 gccctttata ctttttttgg tctggcaaaa aaaccagccc agtccgttga gtctggtgat
     2041 attattgctt tatcaggcgt ggaaaatgtc aaaatcggcg acaccatatc atctttggct
     2101 gaaccgcggc cgctacctcg tctactggtg gatgaaccga ccgtgtccat gatgttttat
     2161 gtcaacgaca gtccttttgg cgggaaagaa gggaaatatc ttacttcgcg ccacctgctg
     2221 gagcggctgg aaaaagagca attgagaaat gtcgccatta aaatcaaaaa actggagcgg
     2281 actgacgcct ttgaagtatg cggacgcggc gagttgcaaa tggcggtatt gattgaaacc
     2341 atgcgccgtg aaggttacga actgatggtg tcgcgtccgc aggtcatcac gcggcaggta
     2401 aacggcaaaa cgtgcgagcc ggtggaaaga ctcttcctgg atatcccgga agaattcgtg
     2461 gggaaaatta ccgaaaaatt atccatcaga aaaggacgca tggaaagtct ggtcaacaaa
     2521 gggtcgggcc gggtcagcat ggagtttctg attccttccc gcggactgat cggctttcgc
     2581 aatcagtttt tgacggatac caaaggcggc ggggtgatga attcactcct ggaagattac
     2641 gcgccgtggt tcggttccat cccgcagcga acgacaggtg tgctggttgc cgaccgctcg
     2701 ggccgcgtta cgccttacgc cagttacgcg atggaagacc ggggtgaaat gattgtggaa
     2761 atcggcacgg atgtttacgc gggcatgatc gtgggcgagc gcaatcgcag ttccgatctc
     2821 aacgtgaaca ttatcaagga aaagaagctg acgaatatgc gggcatccac atccgacgcc
     2881 acggtcattt tacggccgcc gcggatttta tcgctcgatc aggcgatcga attcattgcc
     2941 gaagatgaac tggtggaggt gacgcccaaa agcgttcgct taagaaaaat ggaactggat
     3001 gcaaccaaac gccagatgaa aagccgccgc gaagaataat gtccatcgat cgcgatatcc
     3061 gatatgatca gtaaaattga aaaagatttt taccagatta cgttgcgcat gccctatcgc
     3121 ttaagacacg tgaacgccta tctttttatt tatgaaggaa aactggcgct ttttgacacg
     3181 gggctgaata caccgcaggc ttttgaaact ctgggcaacg atttatccgg catcggtttc
     3241 ggcatcgaag atattcaaca catctatctg acccacgtgc actcggatca ctgcagcatg
     3301 gcgggagtcc tgcaaaaaag gtcgggagca caaattcatc ttgcccaggc ggcttttgaa
     3361 gagtaccggc attttcgcga ggccggtcca accattaaac agttgcgaat ctactatgcg
     3421 cgccagggca tgtcccaaga acaaatcggt ctggttattg aagaatatga aaacattcgc
     3481 ggcatcattg ccgattttga tgccacggat tttctgcgtg ataaagacgt ccgcgaattc
     3541 gggaactgga aaattgaaat aatttctacg cccggacatg ccgccggaca tgtctgcttt
     3601 ttctgcagag agatgaaatt cctgctgtcc ggtgatcatg tgctgccgta cattgcgccg
     3661 gttttaagtc ctgacatctt cgatgaggcg ttccggccgc tggccactta tctggattca
     3721 ctcaacgctt tggaaaacct gtctgtgtct gctgtctatc ccggtcatgg cctggctttt
     3781 acggatttaa aggaaagact gaccgatata cgcgaacatc attccgggag aaaaaaagcc
     3841 gttttggagc atgtgaacaa gcaacccaaa gcaacctgcg agatttcccg ggagatggtc
     3901 agtcccaacc ttgccgattt tgataaattt ctggcgctga atgaaacgat tgcctattta
     3961 aaggaattga ctgctgaagg cgcgataaaa gaagacacgg tcaacgatgt tctggtttac
     4021 gtcaggcctt caggatatta gttgacagat ggatcataat aaggaatata cgccccctga
     4081 ttatataaaa caaagaggca attttgaata aacagttaca aaaggaaatt gaacgccgcc
     4141 gcaccttcgg cattatcagt catcccgatg cgggcaaaac cacactgacg gaaaagcttt
     4201 tgcttttcgg cggggccatt cagatggccg gtgccgtcaa agcgcgcaaa gcgtcgaaac
     4261 atgccctgag cgactggatg gcgattgaaa aagaccgcgg catttccgtg acctcgtccg
     4321 tcatgaaatt cgaatatgca gactgtgaga tcaatctgct ggacaccccc ggccatcagg
     4381 atttttcgga agacacctac cgcgtactca ccgctgtgga cagcgcgctg atggtgatcg
     4441 acggcgccaa aggcgtcgag acgcaaaccc agaagctgat ggaagtctgc cgcctgcgca
     4501 atacgccgat tatcaccttc atcaataaac tcgaccgcga ctgtctgccg ccgctggaaa
     4561 tcctttcgga tatcgaagac aaactgcaaa tcgaatgctc gccgctgtcc tggcctgtcg
     4621 gcatgggcaa gcgtttcaag ggcgtttaca atatgtttga aaagaaactt catttgttta
     4681 cgccggggaa agcgcgccgt tcggaagaag gcatcaccat caacgatttg tccgatccgc
     4741 tgctcgatga attgctggaa agccaggcgg atgaactgcg cgaggaagtc gcgctgctgg
     4801 aaggcgcggc caatccgttt gaattgaagg aatatttgaa agccagccag acgccggttt
     4861 tcttcggcag cgccatcaac aacttcggcg tcaaagaact gctcgatgcg ttcgtggaga
     4921 tcgcgccgcc gcctgccgcc cgaccgacga ccaccagagc cgtggaaccc gaggaagagg
     4981 atttttccgg atttgttttt aaaatccagg ccaacatgga tccggcgcat cgtgatcgca
     5041 tcgccttcct gcggatctgc tcgggacaat ttcagcgcgg catgaaggtc atccatcacc
     5101 gcatcggcaa ggaagtctct ctggccaacg cgacgatttt catggcgcag gaccgaacca
     5161 acatcgaaga agcctatccc ggcgacatca tcggccttca caatcacggc accatcaaaa
     5221 tcggcgacac ctttacgcag aaggaaccgc tcaagttcac cggcattccg cattttgcgc
     5281 cggagcattt ctgccgcgtg cggctcaaag atccgctcag gatgaagcat ctgcaaaaag
     5341 gtctgatgca gctttccgaa gaaggagcga ttcaggtctt caggccctta tggggttcgg
     5401 ataatattct gggcgcggtg ggcgctttgc agtttgatgt cacgatgtcg cgcctcaaag
     5461 acgaatacag cgtttatgcc gattacgaaa agacagacta cgccgccgcc cgctgggtga
     5521 cctgcgacga cgccaaaatc atggaagatt ttcagaagaa aaatcaaacg aacctggctc
     5581 tggataccga agggaatctg gtctatctgg cgttaagcga gtggcgcctc ggcca
//
LOCUS       DAONQZ010000108         5503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_15382_length_5503_cov_15.834985, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000108 DAONQZ010000000
VERSION     DAONQZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15382_length_5503_cov_15.834985"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLR13_11205"
     CDS             <1..183
                     /locus_tag="PLR13_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HQC19950.1"
                     /translation="AENGIIEKSGAWYSYKGDRIGQGRDNSRIFLKENPEVLAQVENE
                     VRVKLGALPKEQEEDK"
     gene            180..677
                     /locus_tag="PLR13_11210"
     CDS             180..677
                     /locus_tag="PLR13_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQC19951.1"
                     /translation="MKVLDLEAAKQKAFRLLSLRPHSEKELEKKLREKGFPAVVIKET
                     LEKLHDLKYLNDASFANQWARNLVVNKLWGNKKVAASLREKGLTAELMALSIEKAREE
                     LPEEAALEILIKKKAAKNKSAALDFKEKQKIFQALMGRGFPPGLILNKLGRIKKEDID
                     GKDWE"
     gene            658..>1292
                     /locus_tag="PLR13_11215"
     CDS             658..>1292
                     /locus_tag="PLR13_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQC19952.1"
                     /translation="MAKTGNEIRESFLKYFESKGHTVVASSSLIPKDDPTLLFTNAGM
                     VQFKNAFLGLENRGYTRAATCQKCARAGGKHNDLENVGVTARHHTFFEMLGNFSFGDY
                     FKEEAIAWAWEYLIDVVKLPQEKLWITIYEDDDEAFEIWNKKMNVPADRIVRMGEKSN
                     FWMMGETGPCGPCSEILFDQGEGTGCGRPECDVHCDCDRHLEIWNLVFTQF"
     assembly_gap    1293..1392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1393..3018
                     /gene="alaS"
                     /locus_tag="PLR13_11220"
     CDS             <1393..3018
                     /gene="alaS"
                     /locus_tag="PLR13_11220"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQC19953.1"
                     /translation="GVYPDLVEKASFITKMILNEEQRFMETLDAGLRILNEETEALKK
                     AGKSVLPGALVFKLYDTYGFPTDLTADIVKRDGFTLDNEGFEKEMEAQRERARGAWRG
                     SGEEAVAECYLKVSSSGVISEFCGYEKIKKDNAEIRAIFIDGKPVETAGEGQKAEIVL
                     DNTPFYGEAGGQTGDTGYLKGAGFNFTVEDTKKPVENFIVHKGVVSKGAINVGAKAKL
                     VVDEERRKAIAANHSGTHILQAALKAVLGDHIKQSGSQVTAEGLRFDFTHFSKIGDEE
                     MKQIEEIANEMIRKNVDVKTEVCALEDALKTGATAVFDEKYGSTVRIVKMGKMSMELC
                     GGTHVKRTGDIGFLKVVHESAIAAGVRRIEAVTGREAFKHFRRTEEELKRTAGLFKAS
                     QMEVYDRAEKLQKHTKELEKEIETLKGKLVAKDSGDLLGQAKEINGVNVLATEVSITD
                     AKTLRDFGDKLRDKLGSGIILLGSKAGDKVMLLCMVTKDLTGKYHAGNIIKELAPMVG
                     GSGGGRPDMAQAGGSQPEKLDQVFPALEKLLAQ"
     gene            3050..4357
                     /locus_tag="PLR13_11225"
     CDS             3050..4357
                     /locus_tag="PLR13_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQC19954.1"
                     /translation="MSLTVQKELFDHRDNKLQLLRSNGLANKIDNGDKMFHDWYRFVL
                     SFPPHLVRDYLYKFQLQNGQTILDPFCGTGTTIVEAKLNHIHSIGIEPNPMAHFASCV
                     KSDWNLDSKELLKHASYIAECANKKIKNSDWDNLRKLPAESEALLLTDSISSLPLHKV
                     LILLEHISHEKESLFYKHELLALAKSLVFSISNLHFGPEVGVHRNKKNDASVIESWLS
                     EIEIMARDIDRADQNDTNSIVHKADSRQVHSLLEPKSIDAVFTSPPYPNEKDYTRTTR
                     LESVILGFINNKKELQSLKRTLLRSNTRNVYKGDDDDLWVAGHVEIQRIADEIESKRI
                     LLGKTSGFEKLYSKVTKLYFGGMARHLAELRTFLKPGAYLGYVVGDQASYLQILIKTG
                     ELLADIAKSLGYEVVSLDLFRTRLATATKKQMREEVVVLRWPG"
     gene            4379..5176
                     /locus_tag="PLR13_11230"
     CDS             4379..5176
                     /locus_tag="PLR13_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19955.1"
                     /translation="MVKPKNRYTLIIESIFSHKYKKGDKEVLFERNDLVQTADKLGIS
                     LPKNLGDVLYSFRYRACLPESIKKFAPEGLEWVIRPIGKAKYKFSLSSMPRIFPNELL
                     AETKIPDATPGIILRYAMNDEQALLAIIRYNRLIDIFTAVTCYSLQNHLRTTVPEMGQ
                     IETDEIYIGVDQRGAQYVFPVQAKGGSDQLGIVQIEQDFALCNSKFTSLICRPIAAQF
                     IKDNCIALFAFEENEHGIAVSSEKHYHLVDPEEMTDDDLQTYRERSF"
     gene            5234..>5503
                     /locus_tag="PLR13_11235"
     CDS             5234..>5503
                     /locus_tag="PLR13_11235"
                     /inference="COORDINATES: protein motif:HMM:NF018339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HQC19956.1"
                     /translation="MERKMQRLPIKLADGQKIKLSPCGQNILIKKIIKDLCSLFTPGG
                     HLIYVGDTNSKCAYYDSNKLAALGVTIKEFSKMPDVVVHHIKKNWL"
BASE COUNT         1673 a         1098 c         1342 g         1290 t
ORIGIN      
        1 gcggaaaacg gcatcattga aaagagcggc gcatggtatt cctacaaggg cgaccgcatc
       61 ggccagggac gggataactc cagaatattt ttaaaggaaa atcccgaggt gctggcgcag
      121 gtggaaaacg aagtccgcgt caaactgggc gctctgccta aggaacagga agaagataag
      181 tgaaagttct cgatttggag gcagcaaagc aaaaggcgtt tcgtcttttg tctttgcggc
      241 cgcattccga aaaagagctg gaaaagaagc tgcgggaaaa aggttttccc gcagttgtta
      301 ttaaagaaac actggaaaaa ctccacgatt tgaagtatct caatgacgcg tcttttgcca
      361 atcagtgggc gcgcaatctg gttgtaaaca aactctgggg aaacaaaaag gtcgcggcca
      421 gtttgcggga aaaaggcctg acggctgaat taatggcatt gtccatcgaa aaggcgcgcg
      481 aggagttgcc ggaagaagcg gcgcttgaaa ttttaatcaa aaagaaagcg gcaaaaaata
      541 agtcggctgc tttggatttt aaggaaaaac agaaaatttt tcaggctctg atgggacgag
      601 gtttcccgcc ggggctgatt ttaaataaat tgggaaggat aaaaaaggaa gatattgatg
      661 gcaaagactg ggaatgaaat cagggaaagt tttttaaaat atttcgaaag caaggggcac
      721 accgtggtgg caagttcgtc gctgattccc aaggacgatc ctactctgct tttcaccaac
      781 gcgggcatgg tacagttcaa aaacgcgttt ctgggactgg aaaaccgggg ctacacccgg
      841 gcggcaacct gccagaaatg cgcgcgcgcc ggcggaaagc acaacgacct ggaaaatgtc
      901 ggcgttaccg cccgtcatca tacctttttc gaaatgctcg gcaatttttc cttcggcgat
      961 tatttcaagg aggaagccat cgcctgggcg tgggaatatc tgattgatgt cgtcaaactt
     1021 ccccaggaaa aattgtggat cacgatttat gaagatgacg atgaggcgtt tgaaatctgg
     1081 aacaaaaaga tgaatgttcc cgcggatcgc atcgtccgca tgggcgagaa aagcaacttc
     1141 tggatgatgg gcgaaaccgg cccctgcggc ccctgctccg aaatcctgtt tgatcagggc
     1201 gaaggcaccg gctgcggccg tccggaatgc gatgtccact gcgattgcga ccgtcatctg
     1261 gaaatatgga atctcgtctt tacgcaattc gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnggcgttta tcccgatctg gtggagaagg cctccttcat caccaaaatg
     1441 attttaaatg aagaacagcg ttttatggaa acgctcgacg cgggtctgcg catcctgaat
     1501 gaagaaacgg aagcgctgaa gaaagccggt aaatcggtcc tcccgggtgc tttggttttt
     1561 aaactctacg acacttacgg cttccccacg gatttgacgg cggatatcgt caagcgcgac
     1621 ggctttacgc tggacaatga aggctttgaa aaagaaatgg aagcgcagcg cgaacgagcc
     1681 cgcggcgcct ggaggggcag cggcgaagaa gcggttgctg aatgctatct caaagtttcc
     1741 agttccggtg ttatctctga attctgcggt tatgaaaaaa tcaaaaaaga caatgcggaa
     1801 atcagggcga tatttattga cggcaaaccg gtggaaacgg cgggtgaagg acagaaggcg
     1861 gaaatcgttc tggacaatac gcctttttac ggagaagccg gaggtcagac cggagatacc
     1921 ggttatctca agggagccgg atttaatttc accgtcgaag atacgaaaaa gcccgtcgag
     1981 aatttcatcg tgcacaaagg ggtggtcagc aagggtgcaa tcaatgtcgg cgcaaaagca
     2041 aaactggttg ttgacgagga aagacgcaaa gccattgccg ccaaccattc cggcacgcac
     2101 atcctgcagg cggcactcaa agcggtattg ggagaccaca tcaagcaatc cggttcgcag
     2161 gttacggcgg aaggattgcg gttcgacttc acccattttt ccaaaatcgg cgatgaggaa
     2221 atgaagcaga ttgaagaaat cgccaacgag atgatccgca aaaatgtgga tgtcaaaacg
     2281 gaagtctgcg cgcttgaaga tgcgctcaaa accggcgcga cggccgtgtt cgatgaaaaa
     2341 tacggctcca ctgtgcgcat tgtgaaaatg ggcaaaatga gcatggagct ctgcggtggt
     2401 acacatgtga aacgcaccgg agatatcggc tttttgaaag ttgttcatga atcagccatt
     2461 gccgcgggcg tacgccgaat tgaagccgtc accggcagag aagctttcaa gcacttccgg
     2521 cgaaccgaag aagaattaaa acgtacggca ggattgttca aagcgagtca gatggaagtc
     2581 tacgaccgcg ccgaaaaact gcaaaagcac accaaagaac tggaaaaaga aatcgaaacg
     2641 ctcaaaggga agctcgtcgc caaggattcc ggcgatctgc tcggtcaggc aaaagaaatc
     2701 aacggagtga acgtgctggc gacggaagtc agcatcaccg atgccaaaac attgcgcgac
     2761 ttcggcgaca agctgcgcga caagctgggc tccggtatca tccttctggg cagcaaggcc
     2821 ggcgataaag tcatgctgct atgcatggtg accaaggatt taaccgggaa atatcatgcc
     2881 ggaaacatca tcaaggaact ggcaccgatg gtcggcggca gcggcggtgg ccgtcccgac
     2941 atggctcagg ccggcggttc ccagccggaa aaattagacc aagttttccc ggcgttggaa
     3001 aaattgttag cacagtgaag taatattagt aaaaaaggta aggtgattaa tgagcttaac
     3061 tgttcaaaaa gaattatttg atcataggga taataaattg caacttctga gatcaaatgg
     3121 ccttgcaaac aaaatcgata atggcgacaa aatgttccat gattggtata gatttgtcct
     3181 gtcattcccg ccccatcttg ttagagatta tctgtataaa tttcaattac aaaatggaca
     3241 gaccatatta gacccgtttt gtggaaccgg cactacaata gttgaagcta aattgaatca
     3301 cattcattct atcggaattg agccgaatcc gatggcgcat tttgcaagtt gtgtaaaaag
     3361 tgattggaat ctcgattcta aggaattgct taaacatgcc tcttacattg ctgaatgcgc
     3421 aaataaaaaa ataaagaata gcgattggga taatttgcgt aaattacccg cagagtcgga
     3481 agctttactt ttaactgatt cgattagttc gcttcccctg cataaggtct tgattcttct
     3541 ggaacacata agccatgaaa aagaatcgct tttttataag catgagttat tggcactagc
     3601 taaatcatta gttttcagta tcagtaattt acatttcggt ccggaagtcg gcgtccatcg
     3661 caacaaaaaa aatgacgctt ctgttatcga aagctggttg tcggaaatag aaattatggc
     3721 gcgtgatata gaccgggctg atcaaaatga cacgaattcg atcgtgcata aagccgattc
     3781 aagacaagtt cacagcctgt tggaacctaa aagcattgat gcggttttta cctctccgcc
     3841 atatcccaat gaaaaggact atacacgtac aaccagatta gagtccgtca ttctaggatt
     3901 tattaataat aaaaaagaac tccagtcatt gaaaagaact ttactacgtt ccaatacaag
     3961 gaatgtttat aaaggcgatg atgatgattt atgggtggcg ggacatgtgg aaattcaaag
     4021 aatagcggac gagatcgaga gcaagagaat attattgggc aagacatccg ggtttgaaaa
     4081 gttatatagt aaagtaacaa agctttactt tggcggaatg gcgaggcact tagccgaatt
     4141 aaggactttt ttaaagcctg gcgcttacct gggatatgtc gttggagatc aagcatccta
     4201 tttacagata ttaattaaaa caggtgaact gcttgcggat atagcaaaat ctttaggtta
     4261 tgaggtcgtg agtttagatt tatttagaac aagattagca accgcaacaa aaaaacaaat
     4321 gcgtgaggaa gttgttgttc taagatggcc tggatgaata cagttaaggg aggcgccaat
     4381 ggtaaaacca aaaaatcgtt acacactcat tatagaaagt atcttttctc acaaatacaa
     4441 gaagggtgac aaggaagttc tgttcgaaag aaatgatctt gttcaaacgg cagacaaact
     4501 gggtatttca cttccaaaaa atcttggcga cgtgttatac agtttccgtt atcgtgcatg
     4561 tttacctgaa tcgataaaaa agtttgcgcc agaaggtttg gagtgggtca ttagacctat
     4621 tggaaaagcc aaatataaat tttcattatc atcaatgcct agaatttttc caaatgaatt
     4681 gcttgcggaa acaaaaattc cggacgccac acctggaata attttgagat acgccatgaa
     4741 tgatgaacaa gccttgcttg caatcataag atataatcgc cttatcgata tatttacggc
     4801 tgtcacttgt tactccttac agaatcacct aaggactacc gttcccgaga tgggacagat
     4861 tgagacggat gagatatata tcggcgtgga tcaacggggt gcacaatatg tttttccggt
     4921 gcaagcgaaa ggtggaagtg atcaacttgg catagttcaa atagagcagg atttcgcttt
     4981 atgtaacagt aaatttacat cattgatttg ccgtccaatt gccgcacaat tcattaaaga
     5041 caattgtatt gcattatttg cctttgaaga gaatgagcat ggcatcgccg tatcatccga
     5101 aaagcattac catcttgtcg atcccgaaga aatgacagat gatgacttgc aaacctatag
     5161 ggaaagatct ttttgatcta gctgaaaaag ccaaatctgt tagaagaact tcctatgggc
     5221 cattggtgaa attatggaaa gaaaaatgca aagattgccc atcaaactcg cagatggtca
     5281 gaaaatcaaa ctttctccct gtgggcagaa tattctaata aagaaaatta tcaaagattt
     5341 gtgttcgcta ttcacacctg gtggtcactt aatctatgtt ggagatacca actcaaaatg
     5401 tgcttattac gattccaata agctggcggc acttggtgtc accataaaag aattcagcaa
     5461 aatgcctgat gttgtcgtac accatataaa aaagaactgg ctc
//
LOCUS       DAONQZ010000109         5281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_16179_length_5281_cov_13.065251, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000109 DAONQZ010000000
VERSION     DAONQZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16179_length_5281_cov_13.065251"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..173
                     /locus_tag="PLR13_11240"
     CDS             3..173
                     /locus_tag="PLR13_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19957.1"
                     /translation="MDVGVNRLENGKLAGDVAFDEVAPKTSAITPVPGGVGPMTIAML
                     MKNTLDAARLML"
     gene            407..2017
                     /locus_tag="PLR13_11245"
     CDS             407..2017
                     /locus_tag="PLR13_11245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQC19958.1"
                     /translation="MVNFIPEFMRVFQSRRLLLTPAIIWFSITFIIAVFLRCLAWSNT
                     AVITPDGAAYIFQAKAIFYSQWKDISDCGMSLKFLTGYPFLIAAVYHVFPDWVIAGRA
                     VNFAFGIATIIPLYLLLRQFLDYRISALTTLIVAVSPFFVGTSVDILRDPLYWFLAVT
                     GLYLFTVCMKDDHRLFLFLSSLFFLLAAWTKHDGLILLAASFIYLLWQDRHIAKIFIF
                     MLPVFAVVALTFIFAGLLNTTISKLNKVDVFVVGLFNIFSSYAALGSELGVLVKSIDG
                     QTQAILKFFLQEAQMNVWLVALGTLMNRLLEASMYVFLIPFMIGFAQLKKIKEDPRLI
                     YFLLLALFILSALYLYVIQTWGLQYRYTAIFTLSSIAFAGIGLETVVKWLSDRYQLKE
                     TLIIILLACMMLFFSLPKNMTARDSDKIVFKEIGEFVVIHEGVQNRGISVSAPMEIQR
                     WISFYANLNYQGAVCPEASEQTSWEMFPSQENLVEQLKQRNIKYFLWAEKSWSERSTK
                     IDGEIEYLKKLGVWRHNDTGKMILFEVI"
     gene            complement(2027..2890)
                     /locus_tag="PLR13_11250"
     CDS             complement(2027..2890)
                     /locus_tag="PLR13_11250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC19959.1"
                     /translation="MKNSTKQCILCGNTSWSLLYSQDQWKVYRCENCNLGVLSPRPDD
                     DELSVLYQQDYFQSQYNQELRIDSPEMMRRLHQETQRLRFFGALKKKGKVLDIGCGRG
                     YFLLACRNRGYQVDGIDLSEDVAEYVRRELHITVHVGAIDRLEIPAESYDAITMWHSL
                     EHTADPNIYLQKARGWLKKDGILIVDVPNYAGHDAKKTWDHWVGWQIPYHLYHFTSDS
                     LKAMLHKHGFTIVRHKHFLSEYVKQKLESLYIPSFIARIIARFYSGHSYAVVARKING
                     RPSPNLQDARR"
     gene            complement(2972..3826)
                     /locus_tag="PLR13_11255"
     CDS             complement(2972..3826)
                     /locus_tag="PLR13_11255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQC19960.1"
                     /translation="MDISVIIVSYNTAAATVECLKSVEAANGAKEIFVVDNASTDESV
                     QKIRQFSPDIHLIVNKRNRGFAAANNQVLPLCRGRYIIYLNPDTIVKPDTPEKAVTFM
                     DGHQNIGLAGAKILNPDGSLQESVSWRYPGEKFTRGETAGLAGNIACVLGAFMIARKS
                     LLDELHGFDEDFFLYGEDQDLAWRIREKGLSIGYIEEAEVLHWGGQSEVQTPPAEIFE
                     KKLHAEYLFYTKHYTKNTIERIKRAQRLKAIFRLFTLHLSLRFSANKSKVQNKIDCYQ
                     LIYKKTNV"
     gene            complement(3848..4936)
                     /locus_tag="PLR13_11260"
     CDS             complement(3848..4936)
                     /locus_tag="PLR13_11260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQC19961.1"
                     /translation="MQIIDSSTFKLPGFVRKVLLIQLGDIGDVVWTTPSIRAVKNSVP
                     NAKVSVLVKEGFGGLLEADPSIEQVFEVKSYRGNLFQQAAGQLSFLRDLRAERFDLAV
                     DLRLGDRGAFTAFATGAPMRVTFYQSWGLPLWRWFFFTHGVVPGPPVYTRGAADQSLR
                     ILREIGIDTKDIIPKLWVSDTVKKRVQELLTREKIGRYDRWITVNPYSRWTYKEWSDF
                     KWIDIIQWLWKEFETPAIFIGSPGENPKVEALIRQCPTPVFNFAGKTTLAELAGLLNL
                     SYLHIGVDSAGPHIAAATGTPTVTIYGPSSWQDWAPIGDQHQVVLPEMDCVPCKRKGC
                     NHSGRSLCLKALSTDSVKSVIHEVMKKL"
     gene            complement(4941..>5281)
                     /locus_tag="PLR13_11265"
     CDS             complement(4941..>5281)
                     /locus_tag="PLR13_11265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQC19962.1"
                     /translation="LYLAGDLYVMLSKFDTFGMVVLEAMAAGLPVMVSSRVGAKDVVA
                     QGENGFVVENPSDHDHVASVLKMMLDEGNRRRMSTAALATASENSWDAAAEKYRTLYE
                     EILAAKKKEA"
BASE COUNT         1298 a         1251 c         1241 g         1491 t
ORIGIN      
        1 tgattgacgt cggcgtcaac cggctggaaa acggcaaact ggcgggcgat gtcgcctttg
       61 atgaagtcgc tcccaagacg tccgccatca cgcccgttcc cggcggcgtg ggtcccatga
      121 ccatcgccat gctgatgaag aacacgctgg acgcggcgag gctgatgttg taatactatt
      181 tggcatagat ttaatccaac agggtgcagt ccaaccccct ccgtttcatg tacaagtcgc
      241 agcaaggctg ccgtaaggat aattttaaaa atcaggagta acgaaacaga gctgaagaag
      301 ctttaaagcg gatatgaaac gatcgatgga ctaaaaatta agacttgact atagactaaa
      361 actatttatg atgaacatta cttttagtca tagaggttgc tgatctttgg ttaattttat
      421 tcctgaattt atgagagtgt ttcaaagcag aaggcttttg ttaactcctg caatcatttg
      481 gttttcaatt acttttatca ttgccgtttt tttacgctgc cttgcctggt ccaataccgc
      541 tgtcataacc cccgacggcg ccgcatatat ttttcaagct aaagcaattt tttacagcca
      601 gtggaaagat ataagcgatt gtggcatgag ccttaaattt ttgacgggat atcctttttt
      661 gattgccgcc gtctaccatg tttttccgga ctgggttatc gcgggaagag ccgtcaattt
      721 tgcttttggt atcgcaacca ttattccgct ttatctgctg ctgcggcaat tcctggacta
      781 tagaatcagc gccctgacga ctctgattgt tgcggtaagc ccattttttg ttggcaccag
      841 cgttgatatc cttagggatc cactctattg gtttcttgcc gtgacgggat tatatctgtt
      901 cacggtatgt atgaaagatg accatcgact tttcttattt ttgagcagcc ttttcttttt
      961 gctggccgca tggacaaaac atgacggact aatcctgctg gccgcatctt ttatttactt
     1021 gctgtggcaa gatcgccata ttgcaaaaat tttcattttt atgctgccgg tttttgcagt
     1081 cgtcgctctc acttttatct ttgccggctt gctcaacacc acgatcagta aactgaataa
     1141 ggttgacgtt tttgtggtcg gcttattcaa tattttttca agctatgctg ctcttggcag
     1201 tgaattgggc gtattggtaa aaagcataga cggacaaact caggcaatct tgaaattttt
     1261 cttacaagaa gcgcaaatga atgtttggct cgttgccttg ggaacactga tgaacagatt
     1321 gctggaagct tccatgtacg tatttttaat ccctttcatg attggttttg cacagctcaa
     1381 aaagatcaaa gaagatcctc gcctgatata ttttctgttg ttggctttat tcatcctttc
     1441 ggcattgtat ttgtatgtca tccaaacatg gggattgcag taccgctata cggcaatatt
     1501 tactttgtca tccattgctt ttgcagggat tgggctggaa accgtcgtca agtggctcag
     1561 cgacagatat caattgaaag agacgctcat cattatcctc ctggcttgca tgatgttgtt
     1621 tttctcgctt cccaagaaca tgaccgccag ggactcggac aaaatcgttt tcaaagaaat
     1681 tggcgaattt gtggtcattc atgagggtgt tcagaaccgg ggcattagtg tttccgcccc
     1741 catggaaatt cagagatgga tatcgtttta tgcgaacctg aattatcagg gtgccgtctg
     1801 ccccgaagct tctgaacaga ccagctggga aatgtttccg agccaggaaa atttggtgga
     1861 gcaactcaag caaagaaaca tcaagtactt cttatgggca gaaaaaagct ggtccgaacg
     1921 cagtacgaag atagatggtg agatcgaata tttaaaaaaa ttgggcgttt ggcggcataa
     1981 cgacacaggc aaaatgatct tgtttgaagt gatatgaaaa aaacaatcac cggcgtgcat
     2041 cttgcaaatt gggagaggga cggccattga tttttctggc cactacggca tagctgtgtc
     2101 cggaataaaa ccgtgcaatg atgcgggcaa taaaggatgg gatatataaa ctttccagtt
     2161 tttgttttac atactctgaa aggaaatgtt tatgccgaac aatggtgaag ccgtgtttgt
     2221 gcagcatggc tttcagagag tcgcttgtga agtgataaag gtgataaggg atttgccatc
     2281 ctacccagtg atcccaggtt ttttttgcat catgtccggc gtaattgggg acatcaacga
     2341 tcaggatgcc gtcttttttc aaccagccgc gcgctttttg cagataaata ttagggtcgg
     2401 ccgtatgttc caaggaatgc cacatggtaa tcgcatcata gctttcagcg ggtatttcca
     2461 ggcggtcgat tgctcctacg tgaacggtaa tatgtaattc cctacggacg tattcggcca
     2521 catcttccga caaatcaatc ccatcaactt gataacccct gttgcggcag gccagcagaa
     2581 agtaaccccg tccacatccg atatccagca ctttgccctt ttttttcaag gcgccgaaaa
     2641 aacgcaaccg ttgtgtttcc tgatggagtc ttctcatcat ttcaggagaa tcaattcgaa
     2701 gttcctgatt gtactgactc tgaaagtaat cctgctgata aagaacagat aattcatcat
     2761 catctggtcg cgggctcagg acaccaagat tgcaattctc gcagcgatag actttccact
     2821 gatcttgaga gtacagcaga ctccagcttg tattgccaca gagaatgcat tgcttcgttg
     2881 aatttttcat gattctcccg aaattttcgg attttgtttc ttctttttgc gccgtttgca
     2941 gatgaccggc ctctgggagg ctaacgcaaa tctacacatt ggtttttttg taaatcaatt
     3001 ggtaacagtc aattttattt tgcacttttg acttgttggc tgaaaagcgc agcgacaaat
     3061 gaagcgtgaa caatctgaat atggctttga gacgctgtgc cctcttaatc cgttcgatgg
     3121 tgttttttgt gtagtgcttg gtgtaaaata aatattccgc atggagtttt ttttcaaata
     3181 tttcggccgg tggtgtctgg acttcgcttt gcccgcccca gtggagcact tccgcctctt
     3241 caatatatcc aatggataac cctttttcac gaatgcgcca ggccagatcc tgatcttctc
     3301 catacagaaa aaaatcttca tcgaatccat gcaattcatc caacagggat ttgcgggcaa
     3361 tcatgaaagc acccagaaca caggcaatgt ttcccgccag cccggcggtc tcgccccgtg
     3421 tgaatttttc tccaggatat cgccaggaaa ccgattcctg aagcgacccg tcgggattca
     3481 gtatttttgc tccggccaat ccgatgttct gatgcccatc catgaaggta accgcttttt
     3541 caggggtatc cggtttcacg atcgtgtcgg gattgagata gatgatgtat cggccgcgac
     3601 ataaagggag aacttgatta ttggcggcgg caaaaccgcg atttcgttta ttcacaatca
     3661 agtgaatatc aggggaaaat tgacggattt tttggacgct ttcatcggtt gaggcgttat
     3721 caaccacaaa tatttctttt gcgccatttg ccgcctcgac ggatttcagg cactcaacag
     3781 tcgcagcggc tgtattatat gaaacaataa tgacggaaat atccatattt ctagtagtat
     3841 tttcccctta tagttttttc atgacctcgt ggatgactga ttttacggag tctgtggata
     3901 atgccttcag gcaaaggctc cggccgctgt ggttacaccc ttttctcttg cagggtacgc
     3961 aatccatctc gggcagaaca acctggtgct ggtctccgat aggcgcccag tcctgccagg
     4021 atgatggccc gtagatggtg acggttggcg tgccggttgc cgcggcaata tgaggccccg
     4081 cgctgtccac accgatatgc agatagctta gattcagaag gcccgccagc tcggccaaag
     4141 ttgttttccc cgcaaaatta aataccgggg tcgggcattg ccggattaat gcctcaactt
     4201 ttggattttc gccgggcgaa ccgataaaga ttgcgggcgt ttcaaactcc ttccacagcc
     4261 attggataat atcaatccac ttgaagtcac tccactcttt gtaagtccat cgggaataag
     4321 ggttaacggt tatccagcga tcgtacctgc ctattttctc cctcgtcagt aattcctgga
     4381 cgcgtttttt aaccgtatca gaaacccata atttgggaat gatatctttt gtatcaatgc
     4441 cgatttctct taagatgcgc aaagattggt ccgccgcgcc gcgtgtatat accggaggcc
     4501 cgggtactac gccatgtgtg aaaaaaaacc atctccagag cggcaatccc caggattggt
     4561 aaaaagttac ccgcatgggc gctcccgttg caaacgccgt aaaagcgccc cggtctccca
     4621 gacgaagatc aaccgccaga tcgaagcgct ccgcgcgaag atcccgtaaa aaagagagct
     4681 gtccggccgc ctgctggaat agatttccac gatagctctt cacctcaaac acctgctcga
     4741 tagaagggtc tgcttccagc aggccgccga agccttcttt gaccagaacg gaaacttttg
     4801 cgttcggaac ggagtttttc actgcgcgga tgctgggcgt cgtccagact acgtcgccga
     4861 tgtcccccag ttgaatcaga agaaccttac ggacaaagcc gggaagtttg aacgtggacg
     4921 agtcgataat ctgcatgtcc tcaagcctct ttctttttgg cggccagaat ttcctcgtag
     4981 agggtccggt atttttcggc cgccgcgtcc caggagtttt cagacgctgt cgccagcgcg
     5041 gcagtggaca ttcggcggcg attgccttca tccagcatca tcttcaggac ggaggccaca
     5101 tgatcgtggt ccgatgggtt ttcgactacg aaaccgtttt caccctgggc caccacatcc
     5161 ttcgcgccca cgcggctgct gaccataacc gggagcccgg ccgccattgc ctcgaggacg
     5221 accatgccga acgtgtcaaa tttggaaagc atcacataca ggtcgcccgc cagatacagg
     5281 t
//
LOCUS       DAONQZ010000110         4970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_17453_length_4970_cov_22.231129, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000110 DAONQZ010000000
VERSION     DAONQZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17453_length_4970_cov_22.231129"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1316)
                     /locus_tag="PLR13_11270"
     CDS             complement(222..1316)
                     /locus_tag="PLR13_11270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQC19963.1"
                     /translation="MKVSILFLIDRLALGGTEGQLKILIQGLDRQRFQPHLGTFNPDN
                     HDDAGVATPHLYLPFSALYSPSLIRCLKKLIKYIRSNDIHIVHTFFQDPTVLAALSRP
                     LHSCKMVGSFRDLGFWRNRQESFKMRLAYPFFDGFIANSHAVKDNFVRFDHIPPEKIT
                     VIHNGISQETITSPLPSLDKHDLPPLVGIVANFNREVKRVDDFILAASIVRQTNPNTR
                     FIVVGEGSLRSRLEKLSQSLGLSELLTFTGQLKDPISIISQLSVGVNTSETEGFSNAI
                     LEYMACGVPVVATNNSGNAELVRDGTNGFLVPIGNVEQMAERISFILCNKELQQTMRS
                     NNLQRVQTEFTVDNMIIAHETFYKNLLAST"
     gene            complement(1620..2990)
                     /locus_tag="PLR13_11275"
     CDS             complement(1620..2990)
                     /locus_tag="PLR13_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQC19964.1"
                     /translation="MSLPAAVYRPRNPQSSDYYRCVEDCFETFVGIYDDHFSRQYGFW
                     RPYIEQVIHRYLDCGDPHNGFARVKCHDCGHEYLLAFSCKRRHFCPSCHQKRVVEFGE
                     WLCVDVLKKIPHRHFVFSIPKILRRYFLYDRKLLADLSRCAWESLKIFLREAAPEKEP
                     IPGAVIAIQTFGDFLGFNPHCHILVTDGCFYGSRGMFRVAPLLELKKLEAIFRHKLFR
                     MLLTKGKITEEMVKMLSAWRHSGFNVFCGNRISPHDETAMENLARYIIRASFSQERMQ
                     YLDQEGKVVYTSKDGKTTKVFPALEWLAAMCSHIPNKGEQMVRYYGFYSNVSRGKRQM
                     TGNDDAVPCILESHGDEKTFRRNWARLIQKIYEVDPLVCPKCKGTMRVIGSIEDPSVI
                     RAILEHLGLWHARARPPPKIHDPPFYGTGRSAAPSIADDVSQIPAHDDHSYGDPQYSW
                     DDYIQA"
     gene            3361..3903
                     /locus_tag="PLR13_11280"
     CDS             3361..3903
                     /locus_tag="PLR13_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQC19965.1"
                     /translation="MKRRILGYVAVMVTVICLCAAPSFAADNYKVGVGYQGMFAGNML
                     NGLSVRGWFGPNTAIGVEGNVFYGAMEVDDTDFDADLWMVEIKAMYAFVVRPNSRFYA
                     GAKFGYGQFSVEPVDFDGKVYIPGILVGAEWNFPSLPEVGFNFDVGYNYILNKNEVNG
                     NDVDLKLHGINVAAGIKYYF"
     gene            complement(4007..4135)
                     /locus_tag="PLR13_11285"
     CDS             complement(4007..4135)
                     /locus_tag="PLR13_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19966.1"
                     /translation="MFNIFDITHDDEPVFFNRHATDITFRINNITGFHHLYFSLKD"
     gene            4309..4683
                     /locus_tag="PLR13_11290"
     CDS             4309..4683
                     /locus_tag="PLR13_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQC19967.1"
                     /translation="MNEQARAVLENHPRTAANVFVRPDGEPFTDIRRRVNPIKEAAGI
                     PADFRALHGLRHTYASMLASSGKVDLYTLQRLLTHKSPVMTQRYAHLRDETLIKASEL
                     AGQIVEQAASANVVKLHEGETS"
BASE COUNT         1281 a         1093 c         1269 g         1327 t
ORIGIN      
        1 gacgaggatg tggcagtgcg gattgaagcc gaggaagtct ccgaaagtct gtatggcaat
       61 gacggcgccg ggaatgggtt ccttctcggg cgccgcttcc cggagaaata tctttctata
      121 ctttgaaccg ttaaaaaaag aaaaaagcct tttagccgtc agtagccact gctgtaggtg
      181 aattggtgcc aaatgaaaaa caaatggcga gcggtggagt gtcacgtact ggctaaaagg
      241 tttttgtaaa atgtctcatg cgcaatgatc atgttatcca cggtgaattc agtctgaaca
      301 cgctgcaagt tgttgctacg cattgtttgt tgtaattctt tgttgcagag tatgaaagaa
      361 atgcgttctg ccatttgttc aacgttgcca attgggacta aaaaaccatt cgtgccgtcg
      421 cggaccaatt cggcattgcc gctattattt gttgcaacaa cagggacccc gcaagccata
      481 tattcgagga tggcattgct aaaaccttct gtttcagatg tgtttacacc gaccgaaagt
      541 tggctgataa tgcttatggg gtctttgagt tgaccggtaa acgtgagtaa ctcgctaaga
      601 ccgagcgatt ggctcagttt ttccaagcgt gagcgtaggc ttccttcgcc aacaacgata
      661 aagcgcgtat tgggatttgt ttgtcggaca attgaggcag cgagaataaa atcgtcgacc
      721 cttttcacct ccctattgaa attggccact ataccaacta gtggcggtaa atcatgtttg
      781 tctagtgagg gaaggggcga tgtaattgtt tcttgggaaa taccattgtg aattactgta
      841 attttttcag gaggtatatg atcgaacctg acaaaattgt ctttcacggc atgggaattc
      901 gcaatgaatc catcgaaaaa ggggtaggcc aatcgcattt taaatgattc ctgacgatta
      961 cgccaaaaac ctaaatcccg aaagctacca accattttac aggaatgcag cggtcgggac
     1021 aaagcggcta atacggtagg gtcttgaaag aatgtatgaa caatatgaat atcattgctc
     1081 ctaatatatt taataagttt ttttaagcac cttataagcg atggggagta aagggcgctg
     1141 aatggtaagt agaggtgtgg ggttgccact cctgcatcgt catggttatc ggggttgaaa
     1201 gtgccgagat ggggttgaaa gcgttgacgg tccaatccct ggattaaaat tttaagttgc
     1261 ccctcggtac ctccaagagc taatctgtct attaaaaaca aaatacttac tttcaaaaaa
     1321 aatcctgtag aatattaaat ttattatgta atttttttgt aaacgtatag atatttttca
     1381 gccattattt tactggtgat tgataagaca ttgcttttga tgggttggag tatatggggg
     1441 atgtttttgc ctgtcaaggg atatttccat cggaaaatca gggttggcga tacggtatgt
     1501 gggtcggcgg tggtccttca gtcgccagcg gctttatttc tgcgcaactt gcacgtcggg
     1561 aaggcaattt gaatcttgtt ttgagcagac atctccggca cgcctctttt cacgaagcgt
     1621 catgcctgga tgtaatcgtc ccaggagtat tgcgggtccc cgtaggaatg atcgtcatgg
     1681 gcggggatct gcgagacgtc atccgcgatg gatggtgccg ccgacctgcc cgtgccgtag
     1741 aacggcgggt catggatttt gggtggcggt ctggcgcgtg cgtgccataa acccaagtgt
     1801 tcgagaatgg cccggatgac ggaagggtcc tcgatgctgc cgatgacccg catggtcccc
     1861 ttgcattttg ggcagaccag gggatcgact tcgtagattt tctgaatcaa acgcgcccaa
     1921 ttccgccgga aggtcttctc atccccatgt gattcgagaa tgcagggtac ggcatcgtcg
     1981 tttcccgtca tctgtctctt tccccgtgag acattgctgt agaagccgta atatctgacc
     2041 atctgctctc ctttattcgg gatgtgggaa cacatggcgg ccagccattc cagcgcagga
     2101 aaaacctttg tggtcttccc gtctttggac gtatagacaa ccttcccctc ctgatccagg
     2161 tactgcatcc gttcctggga gaaagacgcc cggatgatgt agcgggcgag attttccatg
     2221 gccgtttcgt catgcggaga gatacggttt ccgcagaaca cgttgaaccc cgaatgccgc
     2281 catgcggaga gcattttcac catctcttcc gttatcttac ccttagttag cagcatccgg
     2341 aacagcttgt gccggaagat ggcctccagc tttttcagct ccaggagcgg ggcgacgcgg
     2401 aacatgcctc tgctgccata gaaacatccg tcggtgacga ggatgtggca gtgcggattg
     2461 aagccgagga agtctccgaa agtctgtatg gcaatgacgg cgccgggaat gggttccttc
     2521 tcgggcgccg cttcccggag aaatatcttc agcgattccc aggcgcaccg gctcagatcg
     2581 gcaaggagct ttctgtcata gagaaaatat cggcgcagaa tcttcgggat gctgaagacg
     2641 aaatgccggt ggggaatctt cttgaggaca tccacacaga gccattcccc gaactccacc
     2701 acgcgcttct gatggcagga tgggcaaaaa tggcggcgct tgcaagagaa ggccagtaaa
     2761 tactcatggc cgcagtcgtg acatttgaca cgggcaaagc cgttgtgcgg atcgccgcag
     2821 tcaagatacc tatggatcac ctgctcgata tagggccgcc agaaaccgta ttgccgggaa
     2881 aaatgatcgt cataaatgcc gacgaatgtt tcaaagcaat cctccacaca gcggtagtaa
     2941 tccgaagact gcggattcct tggacgataa acggcggcag gaagggacac gatctttctc
     3001 caaaaaagag aacatcatgc cgccataatg tagaacgttc gttctacttg tcaaggcgtg
     3061 gcgtttcttt agagcatccg gtataaattc gtgatagctt tattacagtt attttttttc
     3121 acggcactga aattgtctat tgacaacagc actcgcatgc tttattgttc tcccaacttg
     3181 caattgtttt tagaagctct ttacaagtca tacgtgctct ttcaattttt atttaacgaa
     3241 acccattgtg ttcgtttttg cacctggcat gatgtttgaa gttatggatg atttgaggac
     3301 atttattcat ggtgaataaa tgacttggtc aatttattaa tcaataggag gaaacaattt
     3361 atgaagagaa gaattttggg ttatgtggct gtaatggtca cggtgatttg tctgtgcgct
     3421 gctccgtctt ttgcagcgga caattacaaa gtcggcgtgg gttatcaggg aatgttcgcc
     3481 ggcaacatgc tgaacggcct gtccgtgcgc ggatggtttg gccccaacac cgccatcggt
     3541 gtcgaaggca acgttttcta tggcgcgatg gaagtggatg atacagattt cgatgccgat
     3601 ctgtggatgg ttgaaatcaa agccatgtat gcatttgtgg tgagacccaa cagccgcttc
     3661 tatgccggtg caaaattcgg ttacggtcag tttagcgtag aacccgtcga ttttgatggc
     3721 aaagtctaca ttcccggcat tctggtaggc gctgaatgga atttcccgtc actgcccgaa
     3781 gtaggattca actttgatgt cggatacaat tatatcctca acaaaaatga agtgaacgga
     3841 aacgatgtcg atctgaagct gcatggaatc aatgtagccg ccggcatcaa atactatttc
     3901 taagaagtag tatctaactg atttctttca atccccatgg ccccaatggc catggggatt
     3961 ttttattccc tgaaaataat atcactgata atttcataaa cttgtgttag tcttttaacg
     4021 aaaaataaag gtgatggaag cctgtaatgt tgttgatccg gaatgtgatg tccgtcgcat
     4081 gccggttgaa aaagaccggc tcatcgtcat gggtgatatc gaaaatatta aacattgttt
     4141 ggagtaacaa ataatatatg cttcagattg ttggaataga tatcggttca gtcgcgctgt
     4201 ccgtcgtcgt ggtggatgaa aaaggcgcgg tggtcaactc cttctaccag tttcaggggc
     4261 ttcattgcga ttaagaaccc gaagggcggc gtttctcaga aaatcccgtt gaacgaacag
     4321 gcgcgggcgg tgctggagaa tcacccgcga acggctgcaa atgtttttgt caggccggac
     4381 ggtgaaccct tcacggacat ccggcggcgc gtcaatccga ttaaagaggc ggcgggcata
     4441 ccggcggact ttcgggcgct acacgggctg aggcatactt acgcgtcaat gcttgcctca
     4501 tccggcaagg ttgacttata caccctgcaa aggcttctaa cgcataagtc acccgtcatg
     4561 actcagaggt atgcacatct tcgggatgag acgttgatta aggcttctga attggccgga
     4621 caaatcgttg aacaggcggc aagtgcaaac gtggttaaac tacacgaagg ggaaacatca
     4681 taagacaccg gcttttttat gcgtaagtaa cgcgttaccg aatagatgct cctcggcata
     4741 tttgccgaac aacatttcat tgtctgagtt tcgcgccgcc ataaaaaaag gccgcaatcg
     4801 gacacctacc aattgccaag caattgccat aaaaaccgct ttgtcaacgg gcgttgacac
     4861 tcaaatcccg gcgcttgtgc gcaattgcgc acattatcta ttactttaac tgaatcatag
     4921 taaaaataat ttaaaaaaag ttgttgacaa cattttccgg tatgaaagaa
//
LOCUS       DAONQZ010000111         4920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_17689_length_4920_cov_7.866187, whole genome shotgun sequence.
ACCESSION   DAONQZ010000111 DAONQZ010000000
VERSION     DAONQZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17689_length_4920_cov_7.866187"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1610
                     /locus_tag="PLR13_11295"
     CDS             270..1610
                     /locus_tag="PLR13_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HQC19968.1"
                     /translation="MIFLILPVLLMLNAGAAFAGEALYRNLSAAAKNKDSTLFMELAA
                     SGQTANWTTAEETAFEGNLRFLEGITPADGEPSGLFMVRRLDGQLFIVSIPKAAEMEK
                     TGTAQHYANLKEMLGNKLVFKALLAKGVVNGQPYTYIRFLEPPAQLMLDRIFKISIVL
                     MFFFVMLGMGLNLTVNDFKLVVQKPKGIAVGILLQWFLMPLLALGMAYALGFYHNFPF
                     IYAGLVLICACPGGVTSNLMTYYAKGDLALSISLTSISTLLALFFTPLIMTVLCSNIP
                     DINIPTGLIVQTIIVLVIVPLILGMSIRHKWLNFALKTTPFFSALGVIALLLVIGVGL
                     YTNIEKFAETHRYSVVFYIMVVSLSVMGMLLAAFVPKLFGIENYQTRAISVEVGLRNS
                     TLAITIALLIQDLMGDFYSSMFVTTAIYGITMYITGFLMILLYKKILPLEAQKS"
     gene            complement(1629..2453)
                     /locus_tag="PLR13_11300"
     CDS             complement(1629..2453)
                     /locus_tag="PLR13_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HQC19969.1"
                     /translation="MESQIAKAIHLRYQPVALLWSNEKPEGAMQFTEGKWGCVMWLAA
                     SAAKGKPAAADKKTFGCVGGGVGLGFGNQYLNFPGGEEGFCYFLSSGNAGRPGGAELA
                     EQVKPFMTRESHDNFLNGECYIKNPDGVRKFLDALPIMDIPSACVVFKPLSDVDPAKE
                     KPQTIIFFVNPDQLSALTVLANYGRGDNENVIMPYAAGCQTIGIYPYRESKSARPRAV
                     VGMTDLSARDYVRKQLGDSNFMTFAVPFALFEEMEQNVPGSFLERHTWQNLLKQGR"
     gene            2864..4027
                     /locus_tag="PLR13_11305"
     CDS             2864..4027
                     /locus_tag="PLR13_11305"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HQC19970.1"
                     /translation="MVDKKTARDNQRLSRGRERSGVSFPVAPSIGKMPADYGRLLTEL
                     KERITSERLRVTFAANAAMTLLYWDIGQAILHRQKHEGWGAKVIDRLSADLCEAFPDM
                     HGLSPRNLKYMRKFAESWPDKTIVQASLAQITWYHNLALLEKLDTPDERLWYAAQTVE
                     NGWSRNILVMQIENSLHRRRGKAVTNFKTVMPPADSDMAAQAFKDPYLFDFLGTADPR
                     REREVEQALIDHVQRFLLELGAGFAFVGRQVHLEFSSADYFLDLLFYHLKLRCYVVVE
                     LKAVPFDPAFVGKLNMYLSAVDNLLRHPDDKPTIGLLLCKGKDRLIAEYALRDFRKPI
                     GVADWETKIVSKLPKEFQGSLPTVEEIETELAETAKSDSKTKRKKEAGKNDRN"
     gene            4163..4354
                     /locus_tag="PLR13_11310"
     CDS             4163..4354
                     /locus_tag="PLR13_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19971.1"
                     /translation="MAEGHARKQFEKFEVTHLKYEAQQPTSDFDYFVEKTKKIKIKAV
                     KALPEGKKAKAKTKRKGID"
     gene            complement(4737..>4920)
                     /locus_tag="PLR13_11315"
     CDS             complement(4737..>4920)
                     /locus_tag="PLR13_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19972.1"
                     /translation="SKRQRRGRNPQTGDEITIYIIFSTFEIIDDFPQKTVFLLKTLKV
                     SHSQSLALLIETFMII"
BASE COUNT         1316 a         1181 c         1189 g         1234 t
ORIGIN      
        1 acacaagaga acgtttctta tactgccgac aactaaaagc aagttcttat caatcaatat
       61 taaaatgacg gaaaccggac tcaaaataaa agtcatccgg gaaaaaggcc tgagagaagc
      121 cctgcgatta aagaaaagga aggaatcata ggcctggaga atttaaaaag cacagttcca
      181 ttgcctggcc agtcttgaaa tttagaaaga gaaggagaat gattatgaag ggtaaatcct
      241 ccccatgtcc cgcgcgctgg agatttcgat tgattttttt gatcctgccg gttctgctga
      301 tgttgaatgc cggcgcagct tttgcgggag aagccctcta ccgcaatctt tcagcggccg
      361 caaaaaataa ggattcaacc cttttcatgg aactggccgc aagcggccag acagccaact
      421 ggacaacagc cgaagaaacg gcgttcgaag gaaatctgcg ctttttggaa ggcatcacgc
      481 ccgcggacgg tgaaccctcc ggtctcttta tggtccggcg ccttgacggc cagctcttta
      541 ttgtgtccat cccgaaagcg gcggaaatgg aaaaaaccgg aaccgcgcaa cactatgcca
      601 acctcaaaga gatgctgggc aacaaactgg ttttcaaggc ccttttggca aaaggggttg
      661 tgaacgggca accctacacc tacatccgtt ttctagaacc tccggcccag ctcatgctgg
      721 accggatctt taaaatttcc atcgttttga tgtttttctt cgtcatgctg ggaatgggcc
      781 tcaacctgac cgtcaacgat ttcaagctgg ttgttcagaa accaaaaggc atcgccgtcg
      841 gcattcttct gcagtggttt ctcatgcccc ttctggccct ggggatggcg tatgccctgg
      901 gcttttacca taacttcccc ttcatctatg ccggcctcgt gttgatctgc gcctgccccg
      961 gcggggtcac ttccaatctc atgacctact atgccaaagg ggatttggcg ctttccattt
     1021 cattgacgtc catttccacg ctgctggccc tgttcttcac ccccctgatc atgacggtct
     1081 tatgctccaa cattcccgac atcaacatcc ccaccggcct gattgtccag accatcatcg
     1141 ttctggtgat tgtgcccctg atcctcggca tgtccatccg gcacaaatgg ctcaatttcg
     1201 ccttaaagac cacaccgttt ttctcggctc ttggcgtgat cgccctgctt ctggtcatcg
     1261 gtgtgggact ttacaccaat attgaaaaat tcgcggaaac ccatcgatac agtgtcgtat
     1321 tttatatcat ggttgtttcc ctgtcggtca tgggaatgct gcttgccgct tttgttccca
     1381 aactcttcgg gatagagaac tatcagacca gagccatttc tgtagaggtc ggcctgcgca
     1441 actccacgct ggccataaca attgccctgt taattcagga tcttatgggt gacttctaca
     1501 gctccatgtt tgtgacgacg gccatttatg gcattacgat gtatatcacc ggttttttga
     1561 tgatcctgct gtataaaaag attctgcccc tggaggcaca aaaatcctga aacagggcct
     1621 tctcaggttt aacgtccctg cttcagcaaa ttttgccagg tgtggcgctc caaaaacgaa
     1681 ccggggacgt tttgctccat ttcttcaaac agggcaaacg gcacggcaaa ggtcatgaag
     1741 ttggaatcgc cgagctgctt gcggacatag tcgcgggcgg aaagatcggt catgcccacc
     1801 acggcgcggg gcctggcgga ttttgattcg cgataaggat agatgccgat cgtctggcag
     1861 cccgcggcat acggcatgat cacgttttca ttatcgccgc gtccgtaatt ggccagcacc
     1921 gtcagagcgg aaagctggtc gggattgaca aagaaaataa tcgtctgcgg tttttctttg
     1981 gccggatcga cgtcggaaag cggtttaaag acaacgcagg cagacggaat atccatgatc
     2041 ggcaaagcat ccaggaactt gcggacgccg tcgggatttt taatatagca ttcaccgttc
     2101 aggaaattgt cgtgactctc cctggtcatg aagggcttga cctgctctgc aagctcggcg
     2161 ccgccgggac gtccggcatt ccccgatgac agaaagtaac agaacccctc ttcgccgccg
     2221 ggaaaattca aatactggtt gccgaaaccc agccccacac cgccgccgac acagccgaac
     2281 gtttttttat cggctgcggc aggcttgcct ttggccgcgg aagcggccag ccacatcacg
     2341 cagccccact tgccctcggt aaactgcatg gcgccctcgg gcttttcatt ggaccagagc
     2401 aaggctaccg gctggtatcg aagatgaatc gctttggcta tttggctttc catatcgctc
     2461 cttccagaat gaacacatgc aggaaacttc ctaatggttc aatcattgat ggtttcgtaa
     2521 aaagtcacca ctgtcaccct gagcctagcc tgccctgagc tggccgaagg gaagggtgaa
     2581 tgtaagcact caatatcacg acttgcttcg acgagctcag catgacaaaa gtgtgacaca
     2641 atgacttttt atgagttcat caatcattca ttgtttgatt cttttctaac ccctacaata
     2701 tccctgctta atctgcaagg ttgaaactta aacacctcgt ttttccttgc aatatcgaga
     2761 agaagtagta aaattcgcat caaaatttga gcgaatctat ccccattgaa agacagcgca
     2821 aagattgaca aacagatttg gaaaaacatg aaggaactga gctatggtgg ataagaaaac
     2881 ggcaagagac aaccaacgtc tatcgcgtgg tcgggaacga tccggtgttt catttcctgt
     2941 ggcgccttcc atcgggaaaa tgcctgcgga ctatggacgt cttcttaccg agctgaaaga
     3001 gcgcattaca tcggagcggt tgcgcgtcac ttttgcggct aatgcagcca tgacattgct
     3061 ctactgggac atcggacaag ccattctaca cagacagaaa catgaaggct ggggtgcaaa
     3121 agtcattgat cgcctgtcag ccgatttgtg tgaagctttt ccggacatgc atggactctc
     3181 gccacgcaat ctcaaataca tgcgcaaatt cgccgagtcg tggccggata agacaattgt
     3241 gcaagcgtca cttgcacaaa ttacctggta tcacaatctg gccttactcg aaaaactcga
     3301 tacaccggat gagcgcctct ggtatgcggc gcaaaccgtg gagaacggat ggtcgagaaa
     3361 tattctcgta atgcaaattg aaaacagctt gcatcgtcgg cgtggcaagg ctgtaaccaa
     3421 ttttaagacg gtgatgcctc cggcggattc cgacatggcg gctcaagcat ttaaagaccc
     3481 gtacctgttc gattttcttg gcacggctga tccccggcgc gaacgagaag tcgaacaggc
     3541 gcttatcgat catgtccagc ggtttctgct ggaactcggc gctggttttg cgtttgtcgg
     3601 acggcaggtc catcttgaat tttccagcgc ggattatttc ctcgacctgc ttttttatca
     3661 tcttaagctg cgctgttatg tcgtggtaga gcttaaagcc gtgcctttcg atcccgcctt
     3721 tgtgggaaaa ctcaacatgt atctttcggc tgtggataac ctcttgagac atccggacga
     3781 taaacccaca atcgggctct tgctttgtaa aggcaaagac cggctgatcg ccgaatatgc
     3841 cttgcgggat ttcagaaaac ccatcggcgt ggccgattgg gaaacgaaaa tagtcagcaa
     3901 gcttccgaaa gaatttcaag gcagcctgcc gaccgttgag gagattgaaa cggaactggc
     3961 tgagacggca aaatccgaca gcaaaaccaa gaggaagaag gaagccggta aaaatgatcg
     4021 aaattaagaa agagatcaat ggaaacgtaa agcgagacac gcatatcttg taaggaattc
     4081 ttacaggttc gacaagaagg aagtcgaaaa gtgcgacgtt tggcgaaata ttctgacaca
     4141 ttccggcaga atttcacagg aaatggccga aggacacgcc cggaaacaat ttgaaaaatt
     4201 cgaagtcaca cacctgaagt atgaagctca acaaccgacg agcgactttg actactttgt
     4261 ggaaaaaacg aaaaagataa aaatcaaagc cgtgaaggct ttaccggaag gtaaaaaagc
     4321 aaaagcgaaa acaaagagaa aaggaattga ctgaatcaag aacacgattc tataatcctt
     4381 cctgaaatcg ttatttttga atattaccgg aatatgcagt agttcaaatg gaaagagaga
     4441 attgatgtga gaaaaaagaa gaccggtttg caccggcctt ctttgatgac ggtcaactgg
     4501 gcgttatgtt gacttggcgc tcttgataag ttttaactac aggttaaagt cttttatcaa
     4561 ggattcgata aatcttcatt tacagtggga gcttatatgg ttttgaataa ggtaaaatac
     4621 ctcgatacgt catactatct gatatatagg gtgccattct ttcaggaaga tattctgcaa
     4681 tgaatatatg aggggatgag aactggtatg gctcctgagt ggctatttga agttaatcag
     4741 atgatcatga aggtttctat aagtaatgca agggattgtg aatgggaaac ttttaaggtt
     4801 tttaagagga aaactgtttt ttgtgggaaa tcatcaataa tttcaaaagt tgaaaaaatg
     4861 atgtaaatgg tgatttcgtc acccgtctgc ggatttctgc cccttctttg tcttttgctt
//
LOCUS       DAONQZ010000112         4909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_17741_length_4909_cov_15.682324, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000112 DAONQZ010000000
VERSION     DAONQZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17741_length_4909_cov_15.682324"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..919
                     /locus_tag="PLR13_11320"
     CDS             161..919
                     /locus_tag="PLR13_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQC19973.1"
                     /translation="MRVKDSTDKHKILSLLNKLHPVFCDKPLIRLGAFGDGGYLVPDD
                     LTGIEACFSPGVGFSSVFEKDCADRGMKVFMADGFVNHPSLSHELFVFIKKYVGNTDN
                     DHEMTLDAWVNSSLADATGDLLLQMDIEGAEYDVLSAVSEDLLKRFRIIVIEFHLLDG
                     LRRQPFFNLASQVFEKILRTHTCVHNHPNNCSDAVKVDDIDIPMVMELTFLRNDRIHN
                     PSFVRTFPHPLDCDNTDRNPSLVLPKSWYRNHQK"
     gene            1038..2567
                     /locus_tag="PLR13_11325"
     CDS             1038..2567
                     /locus_tag="PLR13_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQC19974.1"
                     /translation="MFVKVNSMSVIGMESYPVFVEVDTSQGLPQFATVGLPDASVKES
                     RDRIKAAIKNSGYHFPRSHVTINLAPADIKKEGTGFDLPIAVGILAAEKLIREERLRD
                     CLFVGELSLDGSIKRVNGLLSVAFKALETGLTSVFVPEENAPEAAMVKGVNIIPVKTL
                     PDVVECLEGRKTIEPLCLDLEKIFHKTNQYDVDFSEIKGQKQAKRALEIAAAGGHNVL
                     MLGPPGSGKSMLAKRLPTILPDLSFEEAIDVTKVFSVAGFLDKDKTLMAVRPFRSPHH
                     TISDAGLVGGGQMPRPGEISLAHLGVLFLDELPEFRKNVLESLRQPLEDGMITITRSS
                     VTASFPAKFMLVAAMNPCPCGYFGDRVHSCRCTPQQIRQYQSKISGPLLDRIDLHIEV
                     PSVKYRALSDKETGESSAAIKQRVSNARNIQNERFGTNGIMCNARMTEKHIRQFCQVD
                     EEAHKLIDMAMEKLGLSARAVNRILKVSRTIADLEHDESIRTSHVAEAIQYRNLDRGL
                     I"
     gene            2654..3748
                     /locus_tag="PLR13_11330"
     CDS             2654..3748
                     /locus_tag="PLR13_11330"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQC19975.1"
                     /translation="MEIKIKKVPLKKRKTKPADESKLGFGKIFTDHMFTMKYREGEGW
                     YDPVIEPYHPLQLDPTAMCLHYGQEIFEGLKAYRGQDSAIYLFRPMENIKRMNASAER
                     LCMARIDEDLFMEAMKKLVLLEKDWIPHGEGTSMYIRPTMIATEAALGVHPANEYLFF
                     IVVGPVGAYYPQGFSPTKMYVSEEYVRAVRGGIGHVKAAGNYAASLYASRIATNMGYT
                     QVLWLDSVEKKYVEEVGTSNIFFVIGDELITPPLGGSILPGVTRNSVITLARYLGTNV
                     VERPISIEEVIKTSENGSLKEAFASGTAAVISPVGNIYFRDKEYAINGGKTGALAEKL
                     YNEILKIQYGMVEDPFGWRIKIANGKKRSR"
     gene            4016..4717
                     /locus_tag="PLR13_11335"
     CDS             4016..4717
                     /locus_tag="PLR13_11335"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQC19976.1"
                     /translation="MMKFFLNRNKLFTSSWIALSVIVVAISIPGSSFSEERPAIDDVV
                     QKLQLSYESTKDLSADFIQETTVKSIKKTQREEGRVFFKNPQNMSWNYTKPQGKKLVM
                     NHQTAWLYLPQEKVAYRQSAESIFQSRLLIDFFSGSGKLKDDFIITYDNARSPDKDGH
                     YLLVLTPRQKNTTFNVLRLTIDKNNFYILRLSFDDAFGNSTDLRFSRIQFNTGLPKTF
                     FLFKPPKGVQVFEMP"
BASE COUNT         1375 a         1058 c         1198 g         1278 t
ORIGIN      
        1 agcaacattt tttaaatgct tgaagttgaa atagcttgat ataaataggc gaatatttta
       61 agtgaaacat tcgtgggaaa acgggattat cataatcttt agagtattca ccatgaaaat
      121 ttcataaaaa acgggatgaa gtgtattggc agcattgcgt atgcgtgtaa aagattccac
      181 cgataaacac aaaatattat cattattaaa caaattgcat cccgtgttct gcgataagcc
      241 gttgattcgc ctgggagcct tcggtgacgg cggttacctt gtgccggatg atttgacggg
      301 aatcgaagcg tgtttttccc cgggggtggg tttttcatcc gtatttgaaa aagattgtgc
      361 ggacaggggg atgaaagttt ttatggcaga cggatttgtg aatcatccaa gtctgtctca
      421 tgagcttttt gtttttataa aaaaatatgt cggcaatacc gacaatgatc atgaaatgac
      481 cctggacgcc tgggtaaatt catccctggc tgatgccacg ggagatctct tgctacaaat
      541 ggatatcgaa ggtgccgaat atgacgtgtt gtcggcggtg tcggaggatt tgttaaaacg
      601 gttcaggatc atcgttattg aatttcattt gctggacgga ctgcgacgcc aaccattttt
      661 taatctggca tcgcaggtgt ttgaaaaaat tttacggacg catacttgcg tgcacaatca
      721 tcccaataat tgttctgacg ccgtgaaagt ggacgacatt gacatcccta tggttatgga
      781 actgacgttc ttacggaatg atcgtatcca caatccgtcg tttgtcagga cgtttccgca
      841 tcctttggat tgtgataata cggacagaaa tccctctctt gttctaccga aaagctggta
      901 tagaaatcat cagaaatagc cggtcgcgtt tcaggcacgg tttcgacaaa acggtgcgaa
      961 atcgttgaac aagaaaatct cttgatataa atatttctat tggttagtat tcgccccaaa
     1021 aaatataaaa aggcgttatg ttcgttaaag tcaacagcat gagcgtcatc gggatggaat
     1081 cgtatccggt cttcgtcgag gtcgatacat cccagggatt gccgcagttt gcaacggtcg
     1141 ggctgccgga tgcgtccgtc aaagaaagcc gcgaccgcat caaagccgcc atcaaaaatt
     1201 ccgggtatca ttttccccgc agccacgtga ccatcaattt ggctcccgcc gatattaaaa
     1261 aggaagggac cggttttgat ctgccgattg ccgtggggat cctggcggcc gagaaattaa
     1321 tccgtgaaga aagactgcgc gattgtctct ttgtcggaga actttctctg gacggcagca
     1381 ttaaacgggt gaacggtctt ctgtctgttg cgtttaaagc attggaaacc ggcttaacat
     1441 cggtttttgt tcctgaagaa aatgccccgg aagcggcgat ggtgaagggg gtgaacatta
     1501 tcccggtcaa aacgcttccg gatgttgttg aatgtctgga aggacgtaaa accatagagc
     1561 cgctttgcct cgatttggag aaaatttttc acaaaacaaa tcaatacgat gtggatttca
     1621 gtgaaattaa aggacagaaa caggccaaaa gagcactgga gatagcggcg gccggaggcc
     1681 ataacgtcct gatgctgggg cctcctggtt ccggcaagag tatgcttgcg aaaaggctgc
     1741 ccacgatatt gccggatctc tcttttgagg aagccatcga cgttaccaaa gtattttccg
     1801 ttgcgggttt tttggataaa gataaaacgc tgatggcggt gcgccctttt cgttcgccgc
     1861 atcatacgat atccgatgcc gggcttgtgg gcggaggaca aatgccgcgt ccgggagaaa
     1921 ttagccttgc tcatctcggg gtgctgtttc tggatgaact gcccgagttc agaaaaaatg
     1981 ttctcgaatc tctccgccag ccgctggaag acggcatgat aaccattacg cgttcctcgg
     2041 tgaccgcttc ttttcccgct aaattcatgc tggtggcggc gatgaatccc tgtccctgcg
     2101 gttatttcgg cgaccgcgtc cattcctgcc gctgcactcc ccagcagatc cgtcagtatc
     2161 agtccaaaat atccggtccg cttctggacc gaatagattt gcacatcgaa gtgccttcgg
     2221 tcaaatatcg tgcgctgtcc gacaaggaaa cgggcgaatc gtctgcggcg atcaagcagc
     2281 gcgtgagcaa tgcgcggaat atacaaaatg agcgtttcgg cacaaacgga atcatgtgca
     2341 atgcgcggat gacggaaaag cacatccgtc aattctgtca ggtcgatgag gaagcgcaca
     2401 aactgattga catggccatg gaaaaactgg gcttaagcgc ccgcgcggtc aacaggatac
     2461 tgaaagtgtc ccgcactatt gccgatctgg aacatgacga atcgatcaga acgtctcacg
     2521 ttgccgaagc gattcagtat cgaaaccttg atagaggatt gatttagtgc tatgtcaaaa
     2581 aaagactatc acaaccagag cggcgtcctg atgaaatgat tgtccggccg gcagactgga
     2641 aaaagaggta tttatggaga tcaaaataaa aaaagtaccg ctgaaaaaaa gaaaaaccaa
     2701 acccgctgat gaatcaaaac tcggtttcgg taaaatattc acggaccata tgtttaccat
     2761 gaaatataga gagggggagg gatggtacga tcccgtcatc gaaccgtatc atcctttaca
     2821 gcttgatccc acggccatgt gcctgcatta cggtcaggaa atatttgagg gactgaaggc
     2881 ctatcgggga caggacagtg ccatttatct tttccgtccg atggaaaata ttaagaggat
     2941 gaatgcatcc gccgaaagac tgtgtatggc ccgaatcgat gaagatcttt ttatggaagc
     3001 catgaaaaaa ctggtgcttc tggaaaaaga ctggattcct cacggtgaag gaacgtccat
     3061 gtatatccgt ccgaccatga ttgcgacgga agcggccctg ggcgttcatc cggccaatga
     3121 gtatttgttc ttcattgtag tcggcccggt gggcgcctat tatcctcagg gattttcgcc
     3181 gacaaaaatg tatgtttcgg aggaatatgt tcgcgcggtg cgcggcggta tcggtcatgt
     3241 caaggccgcc ggcaattatg ccgccagtct ttacgccagc agaatcgcaa caaacatggg
     3301 gtacacgcag gtgctttggc tggactccgt ggagaaaaaa tacgtggagg aggtcgggac
     3361 cagtaacata tttttcgtga tcggcgacga attgattacg cctccgcttg gaggatcgat
     3421 tctacccggt gtgacgcgta attcagtcat tacgctggcc agatatctgg ggacaaacgt
     3481 tgtcgaaaga ccgatatcga ttgaagaggt tatcaaaacc tcggaaaacg gttcattaaa
     3541 agaagcattt gcttccggaa cggcggcggt catttcgccg gtcggaaata tttacttccg
     3601 cgataaggag tatgcgataa acggcgggaa aaccggtgcg ttggccgaaa aattatataa
     3661 tgaaatcctc aaaattcaat acggcatggt ggaggacccc ttcggctggc gaattaaaat
     3721 cgcgaacgga aaaaaacgtt cccggtaata attttctgtg gacaaaattt gtaaacgcgc
     3781 ttagtccaca agactgtgga aatcgttgtg gacaactttg ttgataaact ctctaagtgc
     3841 gtaattgtta cgattttaca ccaaattgcc caaggtttga gcaatttaaa tatcaagtaa
     3901 aatcaatatc ttattaatat tccctttagg tatcgccatt taagaagtat ttatgaaaca
     3961 agatataaac tctgcgaaaa aaaatgtgcc agaaaagatc gattttttac aaaaaatgat
     4021 gaaatttttt cttaatagaa ataagctatt tacatcgtca tggattgctc tgagcgttat
     4081 tgtcgtggca atttccatac ctggcagctc tttttccgaa gaacgtccgg ccattgacga
     4141 tgttgtacaa aaattgcagc tgtcctatga gtcaacaaaa gatctcagcg cggattttat
     4201 tcaggagaca acggtcaagt cgatcaaaaa gacacagcgg gaagaaggtc gcgttttctt
     4261 taaaaatccc caaaatatgt catggaatta tacaaagcca caggggaaaa aactggtgat
     4321 gaatcatcag acggcctggc tctatcttcc gcaggaaaaa gttgcctacc gacagagcgc
     4381 cgaaagcatt tttcaatccc gactattgat cgattttttt tccggatcgg gaaaactcaa
     4441 agatgatttc atcatcacat atgacaacgc cagatctccg gacaaagacg gccattatct
     4501 tctcgtgctt acgccgcgtc aaaaaaatac gacgttcaat gtgttgaggc ttacaattga
     4561 taaaaacaat ttttatatct tgcggttaag ttttgatgac gctttcggta attcaacgga
     4621 tctgaggttt tcccgcattc aattcaacac cggacttccg aagacgtttt tcctcttcaa
     4681 accacccaaa ggcgtacagg tttttgaaat gccgtgaaaa cccggggcga tgagcaaatt
     4741 tccctttgtc gggttaaaca ttaaggtttg gcgatagtca cccacaggaa agtttcaccg
     4801 cctatactgc ggtattgaaa tgcgtctatt ttaaacccga tggtttccag gattcccctc
     4861 agtttttcag gtgtcgtgtg gctgtagaac agccgttctc ccataaatt
//
LOCUS       DAONQZ010000113         4894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_17802_length_4894_cov_6.496384, whole genome shotgun sequence.
ACCESSION   DAONQZ010000113 DAONQZ010000000
VERSION     DAONQZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17802_length_4894_cov_6.496384"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1547
                     /locus_tag="PLR13_11340"
                     /pseudo
     CDS             291..1547
                     /locus_tag="PLR13_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1796..1987)
                     /locus_tag="PLR13_11345"
     CDS             complement(1796..1987)
                     /locus_tag="PLR13_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19977.1"
                     /translation="MKNVEMKLEGDILTIKVDVTKEFGPSASGKTIIIASTEGNISVP
                     EKDDVKIGLNVYRKKPVAA"
     gene            complement(2026..2778)
                     /locus_tag="PLR13_11350"
     CDS             complement(2026..2778)
                     /locus_tag="PLR13_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19978.1"
                     /translation="MTSKRFEQIGLDRLVHLTWLEKTANLALAGNDAKTIKSILQDEL
                     AGAFRSANKEVRGSLDKTITILLKIWLMPPKDFETLRLLGLELMKQTPLNERMLIHWG
                     MIAAVYPFWSAVAAQIGRLLRLQGTVTAIQIQRRIREQYGERETVSRRARYIIRSFLD
                     WGVIQETNTKGIYKSENTLAVDDIRLIAWLVEASLYARSNGAVPLKDLIDSPALFPFR
                     IKSIRAETLIAVSPRLDFLRHGLDEDLIMLKQ"
     gene            complement(2775..4781)
                     /gene="pglZ"
                     /locus_tag="PLR13_11355"
     CDS             complement(2775..4781)
                     /gene="pglZ"
                     /locus_tag="PLR13_11355"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HQC19979.1"
                     /translation="MNSWRDHVLKEFTPKVSRLTLVSDPDGLLLEEGILEGIRKRGFD
                     LIPFEDHVAFRFAYESKYRSCWDRGEQTDLVVLLRSEIKDIDGLPYDLLQSGRKLFFN
                     VGDVFPKLSHPVIAALDRSYFEILYQAQKLHAPGELGENATKEFILRHVFEIAPELIR
                     QPSNLMQVLLRRHYRGQVIPPVLDERLIQLLQQNKQFVGWPLETIVKDREAFFTFLQE
                     RWPTFLDKEAVHVKKGVYEAHDEYHLALKGPVDLPFDHHDIRIYIDNLFMEGFLHPVQ
                     HEHADELSRSWVAVGIQTNAAEDRSRRLCKLAESLKAAIPAEDAKHSDWSHFARSWAK
                     LMCLVNEEPSSVPATTKSKIRTLQTQIDMGFSTWLFKRYAGLINLPPAPPVMLHHIPR
                     FIARQMSDAAGLKVALLVVDGLSLDQWLVVKNVLDANSSSFVFREKTVFAWIPSITSI
                     SRQAAFSGKMPVFFPGSIYTTEKESLLWSQFWTDQGLSQNEVVYIKGLGDGSLDMVSE
                     MISSPRARVAGLVVDKVDRIMHGMELGAAGMHNQVDQWARQLYLQTLVDLLLDQGFRV
                     YLTSDHGNIDAEGCGRPAEGVIADLRGERVRIYPDISLRTKIKGQFPATIEWNNVGIP
                     DDCVALLAPHRQAFIETNRCTVTHGGLSLEEIIVPFVQIERKIK"
BASE COUNT         1351 a         1168 c         1122 g         1253 t
ORIGIN      
        1 catttgtcat gtcgcctttt attgaagagc ggcacatgca ggacctcatc gaatgtctgg
       61 aaaaggctcg ccggacggtc agctattagc cgcatttctt gccttctgaa tgaccgggcg
      121 ataaaagcgg cccgtcgggt tgaaaaatag gatcgttacg cgaccctgca tgggttacat
      181 agtcctccct gaaaaattta caacatattg atattattac ttgattagcg tatttttgtg
      241 tgctataatt accgggaatt taggaaaagg agctaaaaac cggcaaattt atgcaagcaa
      301 aagacaacag ctttgagcaa tcccatggcg acctgttccg gtcgagcttg gaacagatcg
      361 ttgatagaag ccatccattg tatgcgctgg ccaacaagat cgattggtgg agatttgatc
      421 attcctttgg tgctttgttt gcgcagaaga aaggaagacc ggcattgccg acaagactgg
      481 tcgttggcct tcattatttg aaacacgcct acaatgagag tgacgaatcc gttgtggccc
      541 gattgctaga gaatccatac tggcagtatt tttgcgggtt caaacatttt cagcacactc
      601 tgccgatcga cccgtcatcc atgacacggt ggcgcaagcg tttggggccg gataaactcg
      661 aagaactttt gaccgtgacc atccatacgg ccaaagcaga aaagctttta accgagagac
      721 atttggaaag agtgaatgtg gataccaccg ttcaggagaa agccattgcc tttcccacgg
      781 acgccagact ttaccataag gctcgtcgcg tcctcgtcag attagccaag aagatgcaca
      841 ttgatttacg acagaactac gaacgaaccg gcaagaaagt ctttctcaaa cagggacgat
      901 acgcaagcgc cgggcagtac aatcgatcca agaaagaaac gaagaagctc aagaccatgc
      961 tgggttgtgt tattcgggat attgagagaa aatgtagcaa tccggaggga cgcttagtca
     1021 aagtcttgaa cattgccaga gccatcttcc atcagaaaag aaacgacaag aataaggttt
     1081 acagtgttca tgccccggaa gtggaatgta ttgccaaggg aaaagtccac aaaaagtatg
     1141 aatttggttg caaagtctcc atggtcagca gttccaaaga caactggata ttggccattg
     1201 atgctcttca cggcaatccg ttcgacgggc acacgttgaa ggatgcactg caacaggcga
     1261 aggaacacac cggctggcag cctttgcatg cttattgcga caaaggttat cgaggggttg
     1321 cgaaagagat aaccgataca gaggttcatt tatccgggaa gaagaaaaag agcatgaagg
     1381 ccagtctctg gaaatggttt agcaggcggt cagccattga gccgatcttc ggccatctga
     1441 aatccgatca tcggttggaa cggaatcatc ttcaagggca agatggtgat cggatgaatg
     1501 cgattctgtc cggatgtggt tttaatctga gaaagctcct cagagctttc tttttgcttt
     1561 tttcccaggg gctgttttgg cgatatttta atcatccggc tggatttatc ggtctgagca
     1621 tgagcgaaga tcacttcaaa atgatccccg ctcatgctct ttagaaataa atgacttttt
     1681 cagggacgac tacatacgga atcaggacag ggaagagatt gcggtgctgt atagtatgcc
     1741 aatctgatgt gtttgatagg cgcaacccat caaggttctc atagcttgac gggttttatg
     1801 ccgccaccgg tttctttcga taaacattca gcccgatttt gacgtcatcc ttttccggaa
     1861 cggaaatatt gccttccgtt gacgcaatga tgatggtctt cccggaggcc gacgggccga
     1921 actctttggt cacgtccact ttgatcgtca gaatatcgcc ttccagtttc atttctacgt
     1981 ttttcatctt cccctcctgt gattaaaaat gcgcctgctt ctatgttatt gcttcagcat
     2041 aatcaaatct tcatctaaac cgtgacgcag gaaatcaagc ctgggcgata ccgcaatgag
     2101 ggtttcggcg cgaatagatt ttatccggaa ggggaataaa gcgggactgt ctatcaggtc
     2161 ttttaaagga actgctccgt tggaacgtgc ataaagggat gcttcgacca accaggcaat
     2221 caaccgaata tcatcgacag caagagtgtt ttccgatttg tagattcctt ttgtattcgt
     2281 ttcctgaatc actccccagt ccaaaaacga tcgtataatg tatcgtgccc tgcgggatac
     2341 tgtttccctt tcgccatatt gttcacgaat gcgacgctgg atttgaattg ccgtaacggt
     2401 accctgaagt ctcaacaggc gaccgatttg tgccgccacg gcggaccaaa atggatacac
     2461 ggctgcgatc attccccaat gaataagcat acgctcatta agaggcgttt gtttcatcag
     2521 ttccagcccg agaagacgca aggtttcaaa atctttcggc ggcataagcc atatttttaa
     2581 aaggatcgta atggtcttat ccagggaacc gcgtacctct ttattggctg aacgaaatgc
     2641 acccgctaat tcatcttgca ggatgctttt aatcgttttg gcatcattgc cggcgagagc
     2701 taaattcgcc gttttttcga gccacgttaa atgcacaaga cgatcaagcc caatttgctc
     2761 aaatcttttt gacgtcattt aatcttcctt tcaatctgaa caaatggaac gataatctct
     2821 tccaatgaaa gaccgccgtg agttactgtg caacgatttg tttctataaa ggcttgccga
     2881 tgcggtgcca gaagagcaac gcaatcatcc gggattccaa cattattcca ttcgattgta
     2941 gccggaaatt gcccttttat tttagtgcga agggaaatgt cgggataaat acggacgcgc
     3001 tcgcctcgca aatctgctat cactccctca gccggtcggc cacagccttc tgcatcgatg
     3061 ttgccgtggt cagacgtcag atagacacgg aacccctgat ccaaaagcaa atccacaagc
     3121 gtttgcagat acaattgcct tgcccattgg tccacctgat tatgcattcc ggcagcgccc
     3181 agctccatac cgtgcataat ccggtccact ttgtccacga ccagaccggc aactcttgcc
     3241 ctggggctgg aaatcatttc agaaaccata tccaggctgc catcacccag tcctttgata
     3301 taaacaacct cgttttgtga cagcccttga tccgtccaaa actgcgacca taagaggctt
     3361 tctttttccg ttgtgtaaat gctgcctgga aagaaaacag gcattttacc ggaaaacgca
     3421 gcctgcctgg aaatcgatgt tatggaagga atccaagcaa aaaccgtttt ctcccgaaag
     3481 acgaaagacg atgaatttgc atcaagaaca tttttgacaa ccagccattg atcaagagaa
     3541 agaccatcga caaccagcaa agccactttt aaaccagcag catcactcat ctggcgcgca
     3601 ataaaacgtg gaatgtgatg cagcataacg gggggcgccg gaggcaaatt aatcagtcct
     3661 gcataacgtt tgaaaagcca ggttgaaaaa cccatatcta tctgcgtctg aagtgtccgt
     3721 atcttacttt ttgttgtggc tggaaccgat gagggttcct cgtttacaag acacatcaat
     3781 ttcgcccaac tgcgcgcaaa atgcgaccaa tccgaatgtt ttgcatcttc agcaggaatg
     3841 gccgccttca aactttcagc aagcttacac aaacgtcgag aacgatcctc ggctgcattt
     3901 gtttgaatgc ctacagccac ccatgacctg gacaattcat cggcatgttc atgctgcaca
     3961 ggatgtaaga atccttccat gaagagatta tcaatgtaaa tccgaatatc atgatgatca
     4021 aaaggcagat caaccggccc cttgagggct aaatgatatt catcatgcgc ttcataaacg
     4081 ccttttttca catggacagc ttctttatca agaaatgtcg gccagcgttc ctggaggaaa
     4141 gtaaagaaag cttcacgatc cttaacaatt gtttccaggg gccagcccac aaattgctta
     4201 ttctgctgaa gaagttgaat caacctctcg tctagcacag gggggatcac ttgcccgcga
     4261 tagtgacgac gcaaaagaac ctgcataaga ttcgatggct gcctgattaa ctccggggca
     4321 atttcaaaga catgacggag aataaactcc tttgttgcgt tttctcccaa ttcgccgggc
     4381 gcgtgtagct tctgcgcctg ataaaggatt tcaaaataac ttcggtccaa agcagcgata
     4441 accggatggc tgagctttgg aaaaacatct ccaacattga aaaacaattt tcgacccgac
     4501 tggaggagat catatggcag cccatcaata tctttaattt cggagcgaag caggacaacc
     4561 agatccgttt gttctccccg atcccagcac gaccggtatt ttgattcata ggcaaagcga
     4621 aacgcgacat gatcttcgaa tggaatcaaa tcaaaacccc gtttgcggat tccttcgaga
     4681 attccttctt ccagcaaaag cccgtccggg tcggacacaa gggtcaggcg ggatactttc
     4741 ggggtaaatt cttttaagac atgatcgcgc cagctattca ttaatgattc ccctccacgc
     4801 gaattacaag cagcggcgac atatcgggat agattcggtt tttttgatca agctgttcat
     4861 taaaaatctt ctcttcctga ttcaggaggg ccaa
//
LOCUS       DAONQZ010000114         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_17898_length_4874_cov_15.411081, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000114 DAONQZ010000000
VERSION     DAONQZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17898_length_4874_cov_15.411081"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLR13_11360"
     CDS             <1..321
                     /locus_tag="PLR13_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQC19980.1"
                     /translation="RRQIADRFAEQVAKKEALRRRGIRHQGETVWFGLGMFGIVGWAV
                     AIPTLIGIALGLWIDRTWPSQYSWALMFLVAGVIVGCFNAAYWVKKVRSKIIEDDGED
                     DVES"
     gene            293..616
                     /locus_tag="PLR13_11365"
     CDS             293..616
                     /locus_tag="PLR13_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQC19981.1"
                     /translation="MMEKMTLSLDTKMILYLLASFTAGAALGAAYFTGLWWTVRRLSK
                     VQHPSKLLMGSFGVRIAMVLAGFFLIMHGGHWERLALAMLGFIVVRKILTFALGPQHV
                     AETAY"
     gene            658..1389
                     /locus_tag="PLR13_11370"
     CDS             658..1389
                     /locus_tag="PLR13_11370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQC19982.1"
                     /translation="MHIVQLSQISPDQVILWSWGFITLNVTILYTWLVMAILVVGSWL
                     VTRNLSSEINVSRWQHFLEVVISIIRGEISEMTKKGADHYIPLVGTLFLFICTSNVLA
                     VVPGYVAPTSSITTTAALATCVFIAVPFYGISRNGILHYLKEYFQPTFIFFPFHVMGE
                     LSRTLALTVRLFGNIMSHEKVIGILLAVTPLLFPVVMQILGLLIGVIQAYIFAILSMV
                     YIASALSAEEEHHAPAEIKQESVAA"
     gene            1417..1683
                     /locus_tag="PLR13_11375"
     CDS             1417..1683
                     /locus_tag="PLR13_11375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HQC19983.1"
                     /translation="MDSISLVAMASIIGAGLAMGIGSIGPGLGMGSAIAQALAAIAQQ
                     PDERNSLTRTLFVGLAMIESIAIYCFVISMILIFANPFWSHVIK"
     gene            1697..2467
                     /locus_tag="PLR13_11380"
     CDS             1697..2467
                     /locus_tag="PLR13_11380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HQC19984.1"
                     /translation="MHIDWFVFFCQIFNFLLLMYLLKRFLYGRIIKAMDDREAKIAAR
                     FADADELKKKANEEAELYEKRNQALSETKDKMLNEATMAADAKRKELMEKVREEVDQL
                     KTRWQDMLVREQDAFFYDLRQRAAKQLYETARKALADLADADLEERIMDEFIRRVKAL
                     DEEKSMQIRNAIRGGGNQVTIQSAFGISEQRKVQIEEALKKQISNGFTLRYMKQPEIV
                     SGIELRVNGHKIAWSLNEYLETLVESLTETLQKEAHAA"
     gene            2595..4163
                     /locus_tag="PLR13_11385"
     CDS             2595..4163
                     /locus_tag="PLR13_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HQC19985.1"
                     /translation="MNQETSPLKNQDLKDFLDDTFETMEKVLDEQNPELRQYEIGTVQ
                     FVGRDIARVSGLPHIRAEEMVRFTGNYMGLVFNIDPKEIGVILLDPSENLQVGSEVHR
                     TKRVLDVPVGDGLLGRVVDPLGRPLDGLGEINTVMRLPVERPAPAVMDRAPVTVPLQT
                     GIKVIDALIPVGRGQRELILGDRMTGKTAIAVDTIINQKETGIISIYCAIGKKSADVA
                     KVIAELRDHGVMGSCIVVVATGEDTPGLQFIAPYAATSMGEYFVEQGRDVLIVYDDLT
                     SHARAYREVSLLLRRPPGREAFPGDIFYIHSRLLERSTHMRPELGGGSLTSLPITETE
                     AQDMSSYIPTNLISITDGQIYLSPVLFSKGILPAVDVGKSVSRVGGKTQLPAYRSVAG
                     DLRLSYSQFEELESFSRFGTRLDDSTRRTLERGWRVREILKQGQYKPMRASEQIASLL
                     SVTGGALDKVPTEKIREAETRLLESVNQQLPELCERIEAGKKLEMSDRDHILEKIQPA
                     VAPFEEIEEANANN"
     gene            4150..>4874
                     /locus_tag="PLR13_11390"
     CDS             4150..>4874
                     /locus_tag="PLR13_11390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HQC19986.1"
                     /translation="MQTTESLKRRIKSAGDLLSVVKTMKALAAVSIRQYQKAVESLED
                     YNRTVEMGLQIVLKERMGATMQRKDASIKRLGAIIFGSDQGLCGQLNEQIAVFALDDI
                     NGAGVKKENRKVLAVGARVADYVEDAGQPVEELMTTPSSTAGITPLVQEIIMIIEEWH
                     FRQNVDHFVLFYNDYISGSNYQPYKVQLLPVNRDWLKDIARKKWDSKSLPIFRMDCNR
                     IFSSLIREYLFVSLYRAFAESLA"
BASE COUNT         1148 a         1131 c         1401 g         1194 t
ORIGIN      
        1 cgccggcaaa tagcggatcg ttttgccgag caggtggcca aaaaagaagc gctgcgacgc
       61 cgaggcatca ggcatcaggg cgaaacggta tggtttggtc tgggcatgtt cggcattgtc
      121 ggctgggcgg tggccattcc cacgctgatc ggtattgcgc tgggcttatg gattgatcgc
      181 acctggccca gtcagtattc atgggcgctg atgtttctcg tggccggggt cattgtggga
      241 tgtttcaacg ccgcttactg ggtgaaaaaa gtacgcagta aaattattga ggatgatgga
      301 gaagatgacg ttgagtcttg atacgaagat gatcctttat ttattggcat cgttcaccgc
      361 gggtgcggcg ctcggggcgg cctattttac cgggctttgg tggaccgtgc gcaggctttc
      421 aaaggtccaa catccgtcaa agctgctcat ggggagtttt ggtgttcgaa ttgcaatggt
      481 actggccgga ttttttctga tcatgcatgg cggacactgg gaacggctgg ctcttgccat
      541 gctgggattt atcgttgtcc gaaagatttt aacctttgcc ttggggccgc agcatgttgc
      601 ggaaacggca tattaaaaat ccgggcgcag ccccgggtcg ccggatgagg attccatatg
      661 catattgtac aacttagtca gatcagtcct gatcaggtca tcctttggag ctgggggttt
      721 atcacgctca atgtgaccat tttatatacc tggttggtca tggcgatcct ggtggtcggc
      781 tcatggctgg taacgcgcaa tctttcctcg gagattaatg tatcccggtg gcagcatttt
      841 ctggaagttg tgatttcgat catccgcgga gaaatcagcg agatgacgaa aaaaggcgcg
      901 gatcattaca tcccgctggt cgggaccctt tttcttttca tctgtacgtc aaacgtgctg
      961 gccgttgtgc ccgggtatgt tgcccctacg tcatcgatca ccacgaccgc ggcgctggcg
     1021 acgtgtgttt ttattgccgt tccattttac gggatttcac gcaacggcat tcttcattat
     1081 ctgaaggaat attttcaacc cacattcatc ttcttcccgt tccacgtcat gggagagctg
     1141 tcccggacac tggcgctgac cgttcgactg tttggcaaca ttatgagcca tgagaaagtc
     1201 ataggcattc tgctggccgt tactcccctt ttattcccgg tggtcatgca gatcctggga
     1261 ctgctcatcg gggtcattca ggcctacatc tttgccattc tctccatggt ttatatcgct
     1321 tcggcgctgt ctgctgaaga ggagcatcat gctcctgctg aaataaaaca agaaagcgtt
     1381 gctgcttgat aataatttta taaaggagga gtttttatgg atagtataag tttggtcgcg
     1441 atggcctcga ttatcggtgc gggtttggcg atgggaatcg gttcaattgg ccctgggttg
     1501 ggtatgggat cagccatcgc ccaggctctg gcggctattg cacagcagcc ggatgaaaga
     1561 aactctctga ccagaacact ttttgtcgga ttggccatga ttgaatccat tgccatttac
     1621 tgttttgtta tctccatgat tctgattttc gccaatcctt tctggagtca tgtcattaaa
     1681 tgagggggat ggatccatgc atatcgactg gtttgttttc ttttgtcaga tctttaactt
     1741 cctgttgctc atgtatctgc tgaaacgttt tttgtacggg cggatcatca aggccatgga
     1801 tgacagggaa gcgaagattg ccgcacggtt tgccgacgct gatgaactca agaaaaaggc
     1861 aaacgaggaa gccgagcttt atgagaagcg caatcaggcg ctcagcgaga ccaaggataa
     1921 aatgctcaac gaggcgacca tggcggctga tgcgaagcgc aaggaactga tggaaaaggt
     1981 gcgtgaggaa gtcgatcagc tcaagacacg ctggcaggat atgctggttc gcgaacagga
     2041 tgcttttttc tatgatttgc gtcagcgtgc ggccaaacaa ctgtatgaga cggcgcgcaa
     2101 agcgcttgcc gatctggccg atgcggatct ggaggaaagg atcatggatg agttcatccg
     2161 ccgggtcaaa gctcttgacg aggaaaaaag catgcagata cgcaatgcca tccggggcgg
     2221 cggcaatcag gtaaccatcc aaagcgcctt cggaatttcc gaacagagaa aggttcagat
     2281 agaagaagca ttgaagaagc agatttcgaa cggctttacc ctgcgttata tgaagcagcc
     2341 ggaaatcgtt tcgggtattg aacttcgggt gaacggtcat aaaattgcct ggagcctgaa
     2401 cgagtatctg gaaacgctgg tggaaagttt gaccgaaacg ctccagaaag aggcgcatgc
     2461 ggcataatga gattcgtcga gagcgagtga agcgatgctt gagacgttag tcgaattatt
     2521 cccggagcgg agcgggataa caagttcaac gttcaaggtt aaaagaatat ggcaaatagg
     2581 tcaggaggct tagaatgaat caggaaacaa gcccattgaa aaatcaggat ttaaaagatt
     2641 ttctggatga tacctttgaa acgatggaaa aggtactcga cgagcagaat cctgaattaa
     2701 gacaatatga aatcggcacg gtccagtttg tcggacggga tatcgcccgg gtcagcggcc
     2761 tgccgcacat ccgcgcggaa gaaatggttc gctttacggg aaattacatg gggctggtgt
     2821 ttaatatcga tccgaaggaa atcggtgtaa ttcttcttga tcccagtgaa aacctgcagg
     2881 ttggatcgga agttcaccgc accaaacgcg ttctggatgt gccggtcgga gacggactgt
     2941 taggacgggt ggtcgatccc ctcggacgtc cgcttgacgg tttgggtgaa atcaataccg
     3001 tgatgcgtct gccggtggaa agaccggctc cggcggttat ggatcgcgca ccggttaccg
     3061 tgcctttgca gaccggcatc aaggtgattg acgcgctcat tcccgttgga cggggtcagc
     3121 gcgaacttat cctgggcgac agaatgaccg gcaagacagc cattgccgtc gataccatca
     3181 tcaatcagaa ggaaacggga atcatttcca tttattgcgc catcggcaag aaaagcgccg
     3241 atgtggccaa ggtgattgcc gagctgcgcg atcacggcgt tatgggatcc tgcatcgtcg
     3301 tcgtggccac gggtgaagat accccgggcc tgcagttcat cgcgccttac gcggccacca
     3361 gcatggggga atatttcgtg gaacaggggc gtgatgtgct gattgtctat gacgatctga
     3421 cctcgcatgc ccgcgcttac cgggaagtgt cgctgctgtt gaggcgccct ccgggacgcg
     3481 aagcttttcc cggcgatata ttttatattc attcgcgtct gctggagcgt tccacgcaca
     3541 tgcggccgga actcggcggc ggatcgctga cgtccctgcc cattaccgaa accgaggcgc
     3601 aggacatgtc ctcctatatc ccgaccaacc tgatttccat tacggacggc cagatttatc
     3661 tgtcgccggt tcttttcagc aagggcattc ttccggcggt ggatgtcggt aaatccgttt
     3721 cgcgcgttgg tggaaaaaca cagttgccgg cttaccggtc tgttgccggc gatttgcggc
     3781 tttcctattc gcagtttgaa gagctggaat cgttctcgcg tttcggtacc cgcttggatg
     3841 acagcacgcg acggacgctg gaacgcggct ggcgtgtccg tgagattctc aagcagggac
     3901 agtacaagcc gatgcgggca tccgaacaga ttgcctcgct gctgtcggtg acaggagggg
     3961 cactggataa agtgccaacg gaaaaaatcc gggaagcgga aacgcgcctg ttggaatccg
     4021 tgaatcagca gttgccggaa ctttgcgaac gcatcgaagc aggtaaaaaa ctggagatgt
     4081 ccgatcgcga tcatatcctg gaaaaaatcc aaccggctgt tgcgccgttt gaagagatcg
     4141 aggaagccaa tgcaaacaac tgaatctctg aaaaggagaa ttaaaagtgc gggcgatctt
     4201 ttatcggttg tcaagacgat gaaagccctg gcggctgtga gtatccgtca gtatcaaaag
     4261 gctgtggagt ccctggaaga ttataaccgc accgtggaga tgggattgca aattgtgctg
     4321 aaggaacgca tgggagccac catgcagcgt aaagatgcgt cgatcaagcg cctcggagcg
     4381 atcattttcg gttccgatca gggactgtgc ggccagctca atgagcagat tgccgttttt
     4441 gcgctggacg acattaacgg ggcgggcgtg aaaaaggaaa accgcaaagt actggcggtt
     4501 ggcgcacgtg tggccgatta cgtggaagac gccggacaac cggttgaaga attaatgacg
     4561 acaccaagtt ccaccgcagg aatcacgcca ctggtgcagg aaatcatcat gatcattgaa
     4621 gagtggcatt tccgtcagaa tgttgatcat tttgtgctgt tctataacga ttacatcagc
     4681 ggctcaaact atcagcctta caaggtgcag ctgctgcctg tgaatcgtga ctggctcaag
     4741 gatattgcgc gcaaaaagtg ggattccaaa tccctgccca ttttccggat ggattgcaac
     4801 aggatttttt catcgctgat tagggaatac ctgtttgttt cgctttaccg cgcatttgcc
     4861 gaatcgctgg ccag
//
LOCUS       DAONQZ010000115         4786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_18305_length_4786_cov_17.990066, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000115 DAONQZ010000000
VERSION     DAONQZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18305_length_4786_cov_17.990066"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1968)
                     /locus_tag="PLR13_11395"
     CDS             complement(145..1968)
                     /locus_tag="PLR13_11395"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HQC19987.1"
                     /translation="MKVLVINSGSSSLKYEVFDLNAEQSLMSGAVSRIGTKEADHACA
                     CGDKKQLTKIEAPDHRAALDFVLQAITGMEGTPIKNLEEIDAVAHRIGHGGSLYRGPV
                     VIDQSVIDEIRRLIPLMPLHHPAMLAGIDACQKALPDIPHVAVFDTSFHSAIPDEAAI
                     YGLPHEFFTAGVRKFGFHGNSHEYVASKAAEYLETPMRRLNLISCHLGNGASVCAIQR
                     GHSIDTSMGFSPLEGLIMGTRIGDLDPGIIPYLMRQHHLDIDALDKMLNNKSGLLGIS
                     GVSSDMREIIAAADADNARALLAIKAFCYRIKKYIGAYHGVLGGADALIFTGGIGENN
                     RSIRSRCVQGLEKLGFAIDKVRNDRCEVHAEKPVCDISARYANTQILVIATDEELMIA
                     RQCARALDYTSSIKHHLLATDRRPIRVSVSVRHAHLSKSDVEALFGPGYVLTSKSPLY
                     IDSEFAANETVDLVGPRGRVNNVRIIGPERLKTQVEISRTEEFQLGIDAPVRESGDLD
                     GTPGIILEGPAGRIEIPEGVICAMRHIHMTPEDAELYGVKDKDIVMVRIEGDRELIFG
                     DVLVRVKPNYKLEMHIDTDEANAAELPPVSEGYLVRIESRS"
     gene            2267..3091
                     /locus_tag="PLR13_11400"
     CDS             2267..3091
                     /locus_tag="PLR13_11400"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQC19988.1"
                     /translation="MLPDIKTVELFRIGVLHISPFGVLAATGILTGRALALHRAKQAG
                     IPEEDMDDALLYTLLSAFFFAHIAEAVFYHPDQIMRQGPVYLLKFWKGLSSFGGFFGG
                     LFGFMFFVYRRKIRSPLLFAECILQGLVLGWIFGRLGCTIIFDHPGQLSDFFLAFQQP
                     GGARHNLGFYEFLLTLLVLFPVTLVLHRLQARPGSYTAAVLILYTPVRFFLDFLRLDA
                     AQNGDIRYGALTPGQYGSMILFVLGVAIAYRLMTSLPLPSGIVQPVKKKKKKNKER"
     gene            complement(3148..3981)
                     /locus_tag="PLR13_11405"
     CDS             complement(3148..3981)
                     /locus_tag="PLR13_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC19989.1"
                     /translation="MMSGFSNKLAFMTGGSSGIGLATANLLAAQGCNLVLFARGQKML
                     DEACKTVKTKMKSSSQKVNAVSMDVADNDDVQQKIKLAVEKFGVPDILINSAGVGSGD
                     YFENISYEQFDRLLKINVYGTRNTVSAILPFMKQKGGGHIVNIASVAGLIGMYGYSLY
                     CTSKYAIVGFSECLRSELKRFNINVTLICPPEVKTPFIEEEAKTLPPEARIVKNLAGL
                     LTPEQAAKAIVRGIQGRKFLFVPGLMARSLLFWHRISNGWLTRTPSDLIIKLAARRSR
                     Q"
     gene            4547..>4786
                     /locus_tag="PLR13_11410"
     CDS             4547..>4786
                     /locus_tag="PLR13_11410"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQC19990.1"
                     /translation="MKKTKQTTAAERQYLIEKEKFVPVSQYFGEDTFNRKAMRERLPK
                     EAFKKLMDAIEARKTIDAKTADVVAEAMKKWALEKG"
BASE COUNT         1114 a         1207 c         1270 g         1195 t
ORIGIN      
        1 ctacgtttct gaaatttggg tctcattaaa aaagctctct tttcagggag agcttaaaat
       61 atttatcaca aattgaacac tctggcaaac agggaatcgc gctgccgtcc aatacgcctg
      121 tccttttttg caaaacagtc ccgattaact gcgcgactca atgcgcacca gataaccttc
      181 ggataccggc ggcagttccg ccgcgttggc ctcgtccgta tcaatatgca tttccagttt
      241 ataattgggt ttgacgcgta ccagcacatc gccgaaaata agttcgcggt cgccttcgat
      301 gcgcaccatg acgatatctt tgtccttgac gccgtatagt tcggcgtctt ccggtgtcat
      361 gtggatgtgc cgcatcgcgc agatgacgcc ttcgggaatt tcgatccgtc ccgccggtcc
      421 ctccagaatg atgccgggcg taccgtccag atcgcccgac tcacgcacgg gagcatcgat
      481 gccgagctga aattcctccg tacgggatat ttccacctgc gttttgagtc gttccggccc
      541 gatgatgcgg acgttgttga cccggccgcg gggcccgacg agatcgacgg tttcgttggc
      601 ggcaaattcg ctgtcaatgt agaggggcga tttggatgtc agaacatatc ccggtccgaa
      661 cagcgcttcc acatccgatt tgcttaaatg cgcgtggcgc acggagaccg aaacgcggat
      721 gggacgccgg tccgtggcca gcaggtgatg tttgatggaa cttgtataat ccagcgcgcg
      781 ggcgcactgc ctggcgatca tcagttcctc gtccgtcgcg atgaccagaa tctgggtatt
      841 cgcgtagcgc gcgctgatat cgcagaccgg tttttccgca tggacttcgc aacgatcgtt
      901 gcggacttta tcgatggcaa atcccagttt ctccaggccc tgcacacagc gggaacggat
      961 gctgcggttg ttctcgccga taccgccggt gaaaatcaac gcatcggctc cgcccagaac
     1021 gccgtgatac gctccgatgt atttcttgat gcggtaacag aacgctttaa tggccagaag
     1081 cgcgcgggcg ttgtcggcat ctgctgcggc gataatttcc cgcatatcgg aactcacgcc
     1141 ggaaataccc agaagcccgc ttttgttgtt cagcattttg tccaatgcat cgatgtccag
     1201 atgatgctga cgcatcagat aggggatgat ccccggatca agatcgccga tgcgcgtgcc
     1261 catgatcagc ccctccagcg ggctgaagcc catggaggtg tcgatggaat gtccccgctg
     1321 aatagcgcac acgctggccc cgttgcccag gtggcaggaa atgagattca ggcgtctcat
     1381 cggagtctcc agatactcgg cagcctttga ggcgacatat tcatgcgagt ttccgtgaaa
     1441 gccgaatttc cttacgccgg cggtgaaaaa ttcatgaggc aggccgtaaa tggcggcttc
     1501 gtccggaatg gcgctgtgaa acgaggtgtc gaaaacggcc acatgcggga tatccggcag
     1561 cgctttttgg caggcatcga taccagccag catggcggga tgatgcagcg gcatcagggg
     1621 aataagccgc cggatttcgt caatcacgct ctgatcaatg accacggggc cgcggtacag
     1681 cgagccgccg tgaccgatgc ggtgcgccac ggcgtcgatt tcctccaggt tcttaatcgg
     1741 cgttccttcc atgccggtta ttgcctgaag gacaaagtcc aacgcggcgc gatgatccgg
     1801 cgcctcaatt ttcgtcaact gttttttgtc accgcaggcg cacgcatgat ctgcctcttt
     1861 cgtgccgatg cgcgagaccg cgccgctcat gagcgattgc tcggcgttga ggtcaaaaac
     1921 ctcatatttt aatgatgaac tgccgctgtt gatgacgaga actttcatgt tggatcctct
     1981 tgccggggtt gggttaagag tcctttgctc aaaagaccgg ggacgcggct tgccttgctt
     2041 cccgtatgaa aatgactttc agaaagtata ggaaaagcga aaaggcctgt caatgaaaaa
     2101 tccggacctt ccggttacgg atgtcccgaa cggtttgggg aataaaaaaa ataactgaac
     2161 tccgataaaa catgtgctac aaccagcagg cgatttcccg cttttttgaa aattcaaacc
     2221 cgggatggct gggattccct gatgaaaaga atggaggcat agacttttgc tgcccgatat
     2281 caaaaccgtc gaattattcc gtatcggtgt tttgcacatc agtcctttcg gtgtgctggc
     2341 cgctacaggc attctgaccg gtcgcgcgct ggcgttgcat cgggcgaagc aggcgggtat
     2401 tcccgaagaa gacatggatg atgccctgct ctatacgttg ctgtcggcat tcttttttgc
     2461 acacattgcc gaagccgttt tttatcatcc cgatcagatt atgcggcagg ggcctgttta
     2521 cctgctgaag ttctggaaag ggctcagttc tttcggaggc tttttcggcg gactcttcgg
     2581 gttcatgttt ttcgtttatc gaagaaaaat ccgctcgcct ctgctttttg ccgaatgcat
     2641 ccttcaggga ctggttctgg gatggatttt cggcagactc ggctgcacga ttatttttga
     2701 tcatcccggc caattaagcg attttttcct ggcctttcaa cagccgggcg gcgcacggca
     2761 taatctcggg ttttatgaat tcctgctgac cttgcttgtc cttttccccg tgacgcttgt
     2821 tctgcaccgc ctgcaggcca gaccgggaag ttataccgcc gcggttttga ttctctatac
     2881 gccggtgaga ttttttctgg attttctccg tctcgacgct gcacaaaacg gggatatacg
     2941 gtatggggcg ctcacgcccg gacaatatgg aagcatgatc ctgtttgtgc tgggagtggc
     3001 tattgcgtat cgactgatga cctctctgcc gctgccttcc ggcatcgttc aaccggtcaa
     3061 gaagaaaaaa aagaaaaata aagagcgata gaaagaaact ccacgtcaaa aaacatcgca
     3121 tcagacagaa tatttttgca gatatcttta ttggcgtgat cgtctggcgg cgagtttgat
     3181 aatcaaatca gaaggtgttc gcgtaagcca tccgttgctg atacggtgcc agaacagaag
     3241 agacctggcc atcaagccgg gtacgaagag aaacttcctt ccttggatgc cgcggacaat
     3301 tgcctttgcc gcctgttccg gtgtcagcag accggcaaga ttctttacga tccgcgcctc
     3361 gggcgggagt gtctttgctt cttcttcgat aaaaggagtc ttaacttccg gcggacaaat
     3421 gagcgtcacg ttaatattaa accgcttgag ttcgctgcgc aggcattccg aaaatcccac
     3481 gatggcatat ttacttgtac agtacagaga ataaccgtac atgccgatca atccggcaac
     3541 ggatgcaata ttaacgatgt gacccccgcc cttttgtttc atgaaaggca gaatggcgct
     3601 tacggtattc cgggtgccgt acacattgat tttgagtaac ctgtcgaatt gttcgtagga
     3661 aatattttca aaataatccc cggaaccgac accggcgctg ttgattaaaa tatcgggaac
     3721 gccgaatttt tcgacggcca gtttgatttt ctgctgaaca tcatcattgt ccgcgacgtc
     3781 catggagacg gcgttgacct tctgagaaga gcttttcatt tttgttttga ccgtcttgca
     3841 tgcttcatcc agcatcttct gccctcgggc aaataaaaca aggttgcatc cctgcgccgc
     3901 gagtaaattg gccgttgcca gaccgatgcc gctggaccct ccggtcataa aggcgagttt
     3961 attggaaaag ccgctcatca ttgtcctccc gacatgtcat aatctgatct gaaagaagcc
     4021 tgatccggaa tggttcttgc gcgcccggat tgtttcaatc gctgcacacg attctgcgaa
     4081 agaaagcatc ttcatggcgg cggtttttgt gcgattggac tttctttata tcctaaaatt
     4141 catttttctg cagaagaatt ttaacaagtt ggattgatca ggctggggca ttcttttccg
     4201 gtcggagttt ctctcagggc atttttcggt cgattcgcag ctttctgtag tacaattgcg
     4261 tgcgatgagt gcggattttg cgaaatgtcc gcaggtcaaa gatattccgg ataaaggctt
     4321 gaaaaaaacg tcttttctgg agtataggaa acatcacaac cggtgagtcc ggcgtccgtt
     4381 gaaaaggggg taatgtttcc cggcgaaacg tgcatggata taagctaaac ggagatagtt
     4441 gtacgggcag ggatttgcac gccggcagat cacgaccggt gtccggaaga tcgaggaaaa
     4501 caaatgaccg gaatacacta ttttaaacag aggggctgat acgattatga aaaaaacgaa
     4561 acaaaccacc gcggcagaaa gacaatatct gatcgaaaag gaaaagtttg ttcccgtatc
     4621 gcagtatttc ggggaggaca cctttaaccg aaaggccatg agggaaagac ttcccaaaga
     4681 ggctttcaag aagctgatgg acgcgatcga agcgcgcaaa accatcgacg caaagacagc
     4741 cgatgtcgtg gctgaggcca tgaagaaatg ggctctggaa aaaggg
//
LOCUS       DAONQZ010000116         4742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_18525_length_4742_cov_17.386388, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000116 DAONQZ010000000
VERSION     DAONQZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18525_length_4742_cov_17.386388"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..620)
                     /locus_tag="PLR13_11415"
     CDS             complement(60..620)
                     /locus_tag="PLR13_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19991.1"
                     /translation="MTSVNILRQLPNLFHYADVEKFTGNANVFLTRALAKGFVERVTR
                     GVYINRLREGEPSVEEVACFLRTPSYISCEWALNRHGLILQSPVVCTVLTLDTAVGAA
                     RNIRFGGITIEFSRIAPRLFNGFETRNGFNMALQEKALLDTIYLRQHIPFADELDLDN
                     IQKDKLMSLSELFPAIVRKRLEAMLA"
     gene            complement(617..1474)
                     /locus_tag="PLR13_11420"
     CDS             complement(617..1474)
                     /locus_tag="PLR13_11420"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQC19992.1"
                     /translation="MKTYRETYIRKAEIAQLILLHQLFMQKESRHLIFQGGTAIRWCY
                     GGSRFSEDLDFVTQLAPDAAEKILSGAIKGIEKLMVPHFGPGTVTVNKKSARADALKC
                     FLDFRPEAAREKISIKLEFEGLVAGKLPDVQNHVLSSLSAVAGLMASGEFRVPRPNAI
                     IVAQTPAEILSDKVRALLERRYLKGRDFFDLWYLYAVLKTPVDVAMIQRKFTLYRAVF
                     AAQRNMDYFIKPSKQDKEMMREAIAQDLLRFLPPEVIAVHQPDGFAAFLETARSLFGE
                     LQAKGVRLP"
     gene            complement(1754..1966)
                     /locus_tag="PLR13_11425"
     CDS             complement(1754..1966)
                     /locus_tag="PLR13_11425"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQC19993.1"
                     /translation="MKKYLVVFEKTKTGYSAYVPDLPGRIATGHTKRETEKNMYEARQ
                     LHIEGLSEEKIKLPKMQAVSEVFVFS"
     gene            complement(2407..3297)
                     /locus_tag="PLR13_11430"
     CDS             complement(2407..3297)
                     /locus_tag="PLR13_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19994.1"
                     /translation="MGGDVQLSRLVDVSNADETLKEVIHICRLIKPDFNTDELTEAFR
                     EIQNLFKGNYPGFQACNTKYHDFAHTLMVLLAMARLMHGACLQGISFSYKDINLGMIS
                     ALMHDAGYMQSVDDVNGTGAKFTLIHIERSIQFIQNHYAGKACFADDMNNFSDILQCT
                     GLKTDIPNMKFTTEKIALLGKMLGTADLLGQMADRFYLEKLITLYDEFQEGCVPGFDS
                     KIDLLRKTIGFYSWTKTRLENDFGNVARFMADHFRERWHIEGNLYEETMDRNIDYLKS
                     ILKSSQENTFKSLRRKTITI"
     gene            3622..4137
                     /locus_tag="PLR13_11435"
     CDS             3622..4137
                     /locus_tag="PLR13_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC19995.1"
                     /translation="MSNRKLVVVIFLLIGVFLIVKYDLLTMAKVQMINMIYGTEINKI
                     KSFSISAVIPPHMEKDGLTAESICENMASMLDKASIRCLTKNDGQTTPDNASLTISVQ
                     ALQQPDQQQNYQYIISLEVTKSLPKNNETGTSQEKKIWSASEAGLGNISDIRKEISNI
                     TNEFLKAQAGG"
     gene            complement(4175..>4742)
                     /locus_tag="PLR13_11440"
     CDS             complement(4175..>4742)
                     /locus_tag="PLR13_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQC19996.1"
                     /translation="GKKIIMDHRTDAEIAARVLQGDKEAYSLLVDAYQGPLFNLALRM
                     TGSYADADDLTQEIFFRAYQQLRKFNQEKHFFTWLYTIGINIIRNHIKKRNRDEAFNT
                     AQRYCSESQIKKFEEKENDGLPEDRMIILEMTLRKLPIDVREAIILKYYQNMTFEEIA
                     AITGDSTSAVKMRIYRGLEKIKQTMEKK"
BASE COUNT         1319 a         1113 c         1004 g         1306 t
ORIGIN      
        1 aaaagagcac attttagcat agtatgtcta atattttccg acatacgatg catgataagt
       61 catgcaagca ttgcctccag gcgcttcctg acaattgcgg gaaacaattc agaaagtgac
      121 atcaatttat ccttttgaat gttgtcgaga tccaactcat cagcaaacgg gatatgctga
      181 cgcaggtaga tggtatcgag gagcgccttt tcctgcagcg ccatgttgaa gccgttgcgg
      241 gtttcgaaac cgttgaatag gcggggcgcg atgcgcgaga actcaatcgt aatgcctccg
      301 aaccggatat tccgggcagc gccgactgcc gtgtcgagcg tcagaaccgt gcagacgacc
      361 ggcgattgca ggataaggcc gtgacgattg agcgcccatt cgcaggaaat ataggaaggc
      421 gtccgcagaa agcaggcaac ctcttcaaca ctgggctcac cttcacgaag cctgttgata
      481 tagaccccgc gcgtcacccg ttcaacaaat cctttagcca gcgcccgggt gaggaagaca
      541 ttggcgttgc cggtgaattt ctccacatcg gcataatgaa agagattcgg caattgccgg
      601 aggatattga cggacgtcat ggcaggcgca ctcccttggc ttgcagctcg ccaaagagag
      661 aacgagccgt ttccaggaag gcggcaaatc catcaggctg atggacagcg atcacctccg
      721 gcggaagaaa acgcagcaag tcttgcgcga tagcctcgcg catcatttct ttgtcttgct
      781 ttgaaggctt gataaaataa tccatgtttc tttgagcggc aaagaccgcc ctgtacagtg
      841 taaattttct ctggatcatt gcaacatcca cgggtgtttt caggacggca taaagatacc
      901 agagatcaaa aaaatcgcgg cctttaagat agcgacgctc caggagggcg cgcactttgt
      961 cggaaagaat ctccgccggc gtttgggcga cgataatcgc attggggcga ggcacgcgga
     1021 attctccgga agccatcaga ccggcaacgg cgctcaatga tgaaagaacg tgattttgga
     1081 catccggcaa ttttcccgca accagacctt caaattccag cttgatggaa atcttttctc
     1141 tcgcggcttc cgggcgaaaa tccaaaaagc atttcagcgc atcagcgcga gcgctttttt
     1201 tgttcaccgt aacggtgccg ggcccaaaat gaggaaccat aagcttttca atgcccttga
     1261 tggccccgct cagtattttt tcagcggcat cgggcgcaag ctgcgtcaca aaatcgagat
     1321 cttcggaaaa acggctcccg ccataacacc agcgaatggc cgtgccgccc tgaaagatca
     1381 gatggcggct ttctttctgc ataaaaagct ggtgcagaag gatcaactgc gcaatctccg
     1441 ccttgcggat ataggtttca cgataagttt tcatatacgg ttacgatata atacatattt
     1501 gtattgtaat gtaaaccaaa tagtcatgtt gaggaatgac aactgagacg atatgcggag
     1561 aaattcaaaa atctcgaaat gctgttaaac cgctcggacc cggacgtgag attttatttt
     1621 cggccaaatg gtccttcgtg caatccgcat atcgtaatca acctggagat tcatccattt
     1681 ttcaggggac acgccaaaat acttgccgag gcggagagcc ttatcgacgg taatcgagcg
     1741 ttttccgttt gtattaacta aaaacaaaaa cttcactaac tgcctgcatt ttcggtaatt
     1801 ttattttttc ttctgataaa ccttctatat gtaattgcct cgcttcatac atatttttct
     1861 cggtctcgcg ttttgtatga ccagtggcaa tgcgaccagg cagatctgga acgtaggcac
     1921 tataaccagt ttttgttttt tcaaatacga ctaaatattt tttcatattt ttacctctta
     1981 attttgatct gcgcttgctt caggatgctg tccagtgtgc ctgacgctat atcgaaagct
     2041 ttgcacaatt actattttgt catcgcgagg agcgtaagcg acgtggcgat ctctttaaat
     2101 atcaataact aatgagattg cttcgctgct ctcgcaatga cgaatgtcta ttatgcaaac
     2161 gtttcatatc atccgacgtt ttatggcatg ctattaacat atcaacaaga aaagtcaatc
     2221 gttactcaaa tctctccgtc aaccatgtaa ccactgatga cacagaaaag gacgaccgca
     2281 tcgaaacacc tctcgcgaag cggtgacctg aacaggatcg agccgtaacg cattgttgat
     2341 cgcttatggc cgcggcgcca ttgtggaaaa tgaattaaga catatgtccc gggaattaac
     2401 gatcattcaa atagtaatgg tctttcggcg tagtgattta aaggtatttt cctgagagct
     2461 tttcagaata gatttaagat aatcgatatt tctatccatc gtttcttcat ataaattacc
     2521 ttcaatatgc cacctttctc tgaagtgatc agccatgaac cgagcaacat ttccaaaatc
     2581 attttccaaa cgtgttttgg tccaactgta aaacccgata gttttgcgca aaagatcaat
     2641 tttgctgtca aaaccgggaa cgcagccctc ttgaaattca tcatagagcg tgatcagctt
     2701 ttccagataa aagcggtcgg ccatttgacc cagtaaatct gccgttccca gcatcttgcc
     2761 cagcagggcg attttttccg tcgtaaattt catatttgga atatccgttt tcaaaccagt
     2821 gcattggaga atgtcggaaa aattgttcat atcatcggca aagcaagctt tcccggcgta
     2881 gtgattctgt ataaactgaa tgctccgctc gatatgaata agagtaaatt tagcgcctgt
     2941 gccgttgaca tcatcaacag actgcatata gccggcatcg tgcatgagcg cgctgatcat
     3001 ccccagattg atgtccttgt atgaaaaact gataccctgc aaacatgcgc cgtgcatcag
     3061 gcgagccatg gccagcaaaa ccatcaaagt atgggcaaaa tcatgatatt tagtattgca
     3121 agcctgaaaa cccggatagt ttcccttaaa aagattttga atttctctaa aagcttccgt
     3181 aagctcatca gtgttgaaat caggtttgat caggcggcaa atatgaatga cttctttgag
     3241 tgtttcatcg gcatttgaca catccaccaa tcgcgaaagt tggacatccc cacccataca
     3301 agcctccctg ccggcgattc tgtctttttc aatgatatct tgaacacttt aattgcctta
     3361 gacttataca ttcatgatta aaaaaaatca tctttttttt aatctgggat aaattccttt
     3421 ttatcctttc tgaattttcc tcatcttggt ttgtatggct ggaagatatg ccttcctctt
     3481 gttgaaggat caccatgagc aaagcaggct aaatgactat gaatatggca gtttaacaaa
     3541 tttcccgggc caaattttca ttgacaaata aagctgttcc ctatagttca aaaacagcat
     3601 attcaagaca aggcaaaaac tatgagcaac agaaaactgg tcgttgtgat ctttctattg
     3661 attggcgttt tcttgatcgt aaaatatgat ctgttgacca tggccaaagt tcaaatgatc
     3721 aatatgattt acggcaccga aattaataaa atcaagagct tctccatttc ggccgtgatt
     3781 ccccctcata tggaaaagga tggactcacc gccgaatcga tctgcgaaaa catggcctcc
     3841 atgctggaca aggcaagtat ccggtgcttg acaaaaaatg acgggcagac aacgcccgac
     3901 aatgcctctc tgaccatatc cgttcaggca ttacaacaac ccgatcagca gcaaaactat
     3961 caatatatca tttcgttgga agtcaccaaa agtctcccca aaaataacga aaccggcact
     4021 tcacaggaaa agaaaatttg gtccgcatcc gaggcaggct tgggtaacat tagcgacatc
     4081 cggaaggaaa ttagcaatat tacaaacgaa tttcttaagg cccaagccgg cggttaatga
     4141 caaggtctaa ccacccgcat cgcttgcaaa actgtcactt tttttccatt gtttgcttaa
     4201 tcttttccag ccctctgtaa atacgcattt tgacggcgct tgttgaatcg ccggtaattg
     4261 ccgcaatctc ttcgaaggtc atgttttgat agtattttaa aatgatggct tcccgtacat
     4321 caatcggcaa tttccgcagt gtcatttcca gtatgatcat tctatcttca ggcagcccat
     4381 cattttcttt ttcctcaaat ttttttatct gcgattcgga gcaatatctc tgcgctgtgt
     4441 taaaagcctc atccctgttc cttttcttga tgtgattcct gataatattt atgccgatgg
     4501 tgtaaagcca agtgaagaaa tgtttttcct gattgaattt tcgcaactgt tgataagccc
     4561 gaaaaaatat ttcctgcgtc agatcatcgg catcggcata gctaccggtc atccggagag
     4621 caaggttaaa tagcggccct tgatacgcat cgaccaataa agaatacgcc tccttgtctc
     4681 cttgtaaaac tctggcagca atttcagcat ctgtccgatg atccatgata atctttttgc
     4741 cg
//
LOCUS       DAONQZ010000117         4685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_18801_length_4685_cov_13.458099, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000117 DAONQZ010000000
VERSION     DAONQZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18801_length_4685_cov_13.458099"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLR13_11445"
     CDS             <1..354
                     /locus_tag="PLR13_11445"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HQC19997.1"
                     /translation="AWAETFHPLLFNRWARAILGIEMSPAPLTRKQAEHFFRLVRRND
                     KGPPYRMAGFKEFFIDDLTRESFDVAAETVKALREALSLIWDAFCQEYEGVAAGNLWG
                     RYSRYLCIASGREVS"
     gene            564..1553
                     /locus_tag="PLR13_11450"
     CDS             564..1553
                     /locus_tag="PLR13_11450"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC19998.1"
                     /translation="MDQEIKLIDEKVKAKSEFVERINKAMAGTIIGQKNIIDRLLIAM
                     LCNGHVLVEGVPGLAKTLIVKTLATAIDAQFSRIQFTPDLLPSDLIGTMVLNPKTGEF
                     APRQGPIFANIILADEINRSPSKVQSALLEAMQEHHVTISKERYRLPEPFMVMATQNP
                     IEQEGTYPLPEAAIDRFMLKVKITYPSRDEEVQIIDAALIGVEPKVDKVITPQEIMDT
                     RSLINQIYIDVKLKHYIVSLANATRRPEDFGLHEIKGLIQYGTSPRASIYLAQAAKGH
                     AFIRRRGYVTPDDIKSIGMDVLRHRIILTYEAEAEEQNAETIIQKVFDAIEVP"
     gene            1557..2438
                     /locus_tag="PLR13_11455"
     CDS             1557..2438
                     /locus_tag="PLR13_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQC19999.1"
                     /translation="MLDRSIYKKIRQIEIRSHKAVNQLFAGEYRSVFKGQGLEFYEVR
                     EYVPGDDVRLIDWNVSARLGSPYVKKFIEERQQSIILCVDVSASQDFGSQKLSKNEAS
                     AEIAAVLAFSAIKNKDLVGLLMFSDRIEKYIPPKRGRRHVLLVIREILSFERQGKRTD
                     MAQALRYLNRVNKKSSVLFVISDFLAEGYEQPIRVLSKKHDLIPIIIKDKMEKQLPQL
                     PMVCFEDPESGELIDVDLRDEKLRQAYHDASTDTLNGLRKYFRSLKLDHLELETDGQY
                     IAALNRFFKVRERRIAH"
     gene            2438..2710
                     /locus_tag="PLR13_11460"
     CDS             2438..2710
                     /locus_tag="PLR13_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20000.1"
                     /translation="MPEQLKDIKGVIYVFDWGTFLFFLLITLLLAIAGYFLFKLFKKR
                     RRNRKDKSNAEIIPERPCNEVALEALMKIDPVEYFEKKRSRNIISK"
     gene            2800..2955
                     /locus_tag="PLR13_11465"
     CDS             2800..2955
                     /locus_tag="PLR13_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20001.1"
                     /translation="MERDFDKVKKVDRYFSDCDLVKFAKLQPGLAEMKQKKTESENIV
                     KECGKKV"
     gene            2959..3633
                     /locus_tag="PLR13_11470"
     CDS             2959..3633
                     /locus_tag="PLR13_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQC20002.1"
                     /translation="MRFQSFWFIVLLFALPFFYRFVVKKKPASLPHPDFISKLKNRIP
                     QATVKNKLPLYLRLAVLVLLILALMRLQYGYVADTSNRNGVDIMVALDVSTSMNAEDF
                     QPDRITVARDVLKNFIQGRPQDRVGLVIFSGKSYLQSPLTTDHNEVLNYLDQIHTGMI
                     EDGTAIGMALATSVNRLKDSEAKTKIILLLTDGENNSGEIDPETAAKMAQALGIKIYS
                     VGISQR"
     gene            3754..>4685
                     /locus_tag="PLR13_11475"
     CDS             3754..>4685
                     /locus_tag="PLR13_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20003.1"
                     /translation="MRKNVLWIASCAIVFFSLNACDKTIGVDDELKPTKLTVEQQKQK
                     IEDNGIAFLDKMDGMKETVAYKTMTDFVEENASKASPQMRQLLAAMESKNLKNINVLS
                     TQLKSVKEDDDDVWGEWLWDADVEDFEKVKDLTDKIIVKFPAADSKTNNAELTVNYTE
                     SKVEIPDADEYYPSDVTCQFKVDGSMALEAGFHGTYKTDGTPTSATNTLKVGDYKWEA
                     TLKNDSKVASETYEFTYKSETLIKMKGEINGDLTYGSLEEAVEDEEAAMDMLSSGEIY
                     FQMMDLAILGSITDLQKMIDEVEDLEDSNLSETD"
BASE COUNT         1361 a         1026 c         1138 g         1160 t
ORIGIN      
        1 gcctgggcgg agacgtttca tccgctgctc ttcaaccgct gggcccgcgc catccttggc
       61 attgaaatgt ctccggcgcc cctcacaagg aaacaggcgg aacatttctt ccgtctggtc
      121 cgcaggaatg ataagggacc gccctaccgg atggccggat tcaaggaatt ttttatcgat
      181 gacttaacca gggaatcctt tgatgttgca gcggaaacag tgaaagccct ccgggaggca
      241 ctctccctga tctgggacgc cttttgtcag gagtacgaag gtgtcgctgc cggcaatctg
      301 tggggtcggt attcgcgata tctctgcata gcttcggggc gggaggtttc gtaaccgcgt
      361 cgaaccgact tacgtcatgg aattgggcga cgcgatccgt tcgccggctt tgtaagggaa
      421 acgaaaaata gttacagggc ggtgtaaggc attatctcaa acggcgaaag atatttccga
      481 cccaagatat caaagaagaa aaagttgatg ggtcagcttt tacagttaag ttgtaaaata
      541 ttttttagag aggggaatta tttatggatc aggaaataaa actcatcgat gagaaggtga
      601 aagccaaaag tgaatttgtc gaaagaatca ataaagcaat ggccggcacg attatcggcc
      661 agaaaaatat tatcgaccgc ctgcttattg ccatgctctg caacggtcat gtcctggtgg
      721 aaggcgttcc aggactcgcc aagaccctga ttgtcaagac gctggccact gccatcgacg
      781 cccaattttc gcgtattcag tttacgccgg atcttctgcc gtccgacctg atcggcacga
      841 tggtcctcaa tcccaaaacc ggggagtttg ccccccgtca ggggccgatc ttcgccaaca
      901 tcattctggc ggatgaaatc aaccgctctc cttccaaagt tcaaagcgca ttgctggaag
      961 ccatgcagga gcatcatgtc acgatttcca aggaaaggta tcgtttgcct gagcccttca
     1021 tggtcatggc cacgcaaaat cctattgaac aggaaggaac ctatcctctg ccggaagcgg
     1081 ccatcgaccg cttcatgctc aaggtgaaaa taacgtatcc gagtagggat gaagaagtgc
     1141 agatcatcga cgcggcgctt atcggtgtgg agccgaaagt ggataaagtc atcacaccgc
     1201 aagaaatcat ggacacccgc agcttgatta atcaaatcta tattgatgta aagttgaaac
     1261 actatattgt ttctctggcc aacgccaccc gccggcccga agatttcgga ctccatgaga
     1321 tcaagggatt gattcagtac ggcacttcgc cacgcgcctc catttatctg gcccaggccg
     1381 ccaaaggtca cgcgtttatc cgtcgccgcg gatacgtcac gcccgacgat atcaaaagta
     1441 tcggcatgga tgttttgcgg caccggatta ttctgactta cgaagccgaa gccgaggaac
     1501 agaacgcgga aaccatcatt caaaaagttt tcgacgccat tgaggtacct taatccgtgc
     1561 tggaccgttc gatttacaaa aagatccggc aaatcgaaat ccggtcgcat aaggcggtca
     1621 accagctctt tgccggagaa taccgcagcg tcttcaaggg acaggggctg gaattctacg
     1681 aagtccggga gtacgttccg ggagacgatg tccgcctgat tgactggaat gtcagcgccc
     1741 gcctcggttc tccctacgtc aaaaaattca tcgaggagcg gcagcagagc attatccttt
     1801 gcgtggatgt cagcgcttcc caggatttcg gatcgcaaaa attgagcaag aacgaagcat
     1861 ccgcggagat tgcggccgtg ctcgcttttt ccgctataaa aaacaaagac ctggtcgggc
     1921 ttttgatgtt ttccgatcga attgaaaaat acattccgcc caaacgggga agacgtcatg
     1981 tgcttttggt catcagagag atactttctt ttgaacgtca ggggaaaaga accgatatgg
     2041 cgcaggccct gcgctatcta aaccgtgtca ataaaaaaag ttccgtctta tttgtaatat
     2101 cggattttct ggccgagggg tatgaacagc cgatccgtgt tctatctaaa aaacatgatc
     2161 tcattcccat catcatcaag gataaaatgg aaaaacaact gccccaacta ccgatggttt
     2221 gttttgaaga tcctgagtcg ggtgaactga tagacgttga tttgcgggat gaaaaactac
     2281 gccaagccta ccatgatgct tctacagaca cattgaacgg tttgcgaaaa tatttccgct
     2341 cgctgaaact ggatcatctg gaactggaaa cggatggtca atacatcgcc gcgctcaacc
     2401 gctttttcaa agtgcgggaa agaaggatag ctcactgatg ccggaacaat taaaagatat
     2461 caaaggcgtt atctatgttt ttgactgggg cacgtttttg ttttttcttt tgatcacctt
     2521 gctgttggcc attgccggat atttcctttt taagcttttc aaaaagcggc gtcggaaccg
     2581 caaagataaa agcaacgcag agattattcc ggaaaggccc tgtaatgaag ttgcgctgga
     2641 ggcgctgatg aagattgatc cggtcgagta ttttgaaaaa aaaagatcaa ggaatattat
     2701 ttcgaaataa ccgatgttat ccgcaaattc cttggctccc actatcatat tgacacgctg
     2761 gataaaacgt ctctggaaat tattgaagga ctggagcgca tggagcgcga ttttgacaag
     2821 gtaaaaaagg ttgatcgcta cttcagcgat tgcgatctgg tgaaattcgc caagctgcaa
     2881 ccgggattgg ccgaaatgaa acaaaagaaa acggaatcgg aaaatatcgt taaggaatgt
     2941 gggaagaagg tgtagggaat gcgatttcaa agtttctggt tcattgtttt gttatttgct
     3001 ttgccttttt tctaccggtt tgttgtgaaa aaaaagccgg ccagcctgcc gcatcctgat
     3061 ttcataagca agctcaagaa tagaattcct caggcaaccg ttaaaaataa gctgccgtta
     3121 tacctgaggc tcgcggtgct ggtacttctg attctggctt tgatgcgcct ccagtacggg
     3181 tatgttgccg acaccagcaa ccgcaacggt gtggatatta tggtggcgct ggatgtcagc
     3241 accagcatga acgcggaaga cttccagccc gacagaatca cggtcgccag ggatgttctg
     3301 aaaaacttta ttcaaggccg cccgcaggac cgcgtcggtc tggtgatatt ttccggtaaa
     3361 agttatctgc aatccccgtt gaccaccgat cacaacgagg ttttaaacta tctggatcag
     3421 attcataccg gcatgattga agacggcacc gccatcggca tggcgctggc cacttccgtc
     3481 aaccggctga aggattcgga agccaaaaca aaaatcattc ttcttttgac cgatggggaa
     3541 aacaactccg gcgaaattga tccggaaaca gccgccaaaa tggcgcaggc tctgggtata
     3601 aaaatatatt ccgtcggcat ctctcaacgt tgataataag tgagttgatt tttagcttat
     3661 ttaaggtcaa aaatattcgt tttgccgttt gaaatatttt gtacactttt gcaatgctta
     3721 ctaatatgag ttacaattta taaaaccaaa aaaatgagaa aaaatgtatt gtggattgcc
     3781 tcgtgtgcaa ttgtattttt tagcttaaat gcttgtgata aaacaattgg tgttgacgac
     3841 gaattgaagc caacaaaact aactgtagaa cagcaaaaac aaaagatcga agacaatggg
     3901 attgcattcc tcgacaagat ggatgggatg aaagaaaccg ttgcctataa aacaatgacc
     3961 gactttgttg aagaaaatgc gtcgaaagct tcgccacaaa tgcgccaatt actagcggcc
     4021 atggagagca agaacctgaa aaacatcaat gtgctgagta ctcaattaaa atcggttaaa
     4081 gaagatgacg acgatgtttg gggcgaatgg ttatgggatg ccgatgtaga agattttgag
     4141 aaagttaagg atttgaccga taaaataatt gtgaaattcc ctgctgctga ttctaaaaca
     4201 aacaatgccg aattgactgt taactacacc gagtcgaaag tagagattcc agatgcagac
     4261 gagtattatc cttctgatgt aacttgccaa ttcaaggttg atggttcaat ggcattagaa
     4321 gctggattcc atggaactta caaaaccgac ggcacgccta catcggctac aaatacctta
     4381 aaagtaggcg attacaaatg ggaagcaacc ttaaagaacg attcaaaagt agcttctgaa
     4441 acatacgagt ttacctataa gtcagaaaca ttgatcaaaa tgaagggtga aatcaatggc
     4501 gaccttacat acggaagtct tgaagaagcc gttgaagatg aagaagctgc aatggatatg
     4561 ctaagcagtg gtgaaattta tttccagatg atggatttgg ccattttagg aagtattaca
     4621 gacttgcaga aaatgattga tgaagtagaa gaccttgaag attcaaacct ttcggaaact
     4681 gacga
//
LOCUS       DAONQZ010000118         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_18897_length_4666_cov_18.337237, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000118 DAONQZ010000000
VERSION     DAONQZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_4666_cov_18.337237"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLR13_11480"
     CDS             complement(<1..371)
                     /locus_tag="PLR13_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQC20004.1"
                     /translation="MELMHRYNVLKQKITDRKEPFDQFVNLLENKTTWLTSPASTRYH
                     LAVECGLLKHSVGVAETILRFREFLAPSISEESCVIVGLFHDVGKLGVPDKPYYLPND
                     NAWLVKNRGICYKTNPDVVAMG"
     gene            complement(461..2725)
                     /locus_tag="PLR13_11485"
     CDS             complement(461..2725)
                     /locus_tag="PLR13_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC20005.1"
                     /translation="MNQTQNAVRNQPAPPGGQAGQRLAIVPDGEATLEESPFADNYEY
                     LAALEKEALLMLVLSAVRSGKAEGLENDGEGIRRCAGLGLTPQNITWEHIEAELARVR
                     HINRSRQNKTLRSGVRLFFPLFCRENQLDEFDEKILLLLFMQATSETFRNAFCPCGLN
                     DDAGGIIIRVMLFILCKDYMEQLEKKRHFSRGAPLVAREIIFFKSDDECRSSHVMDET
                     VTINERHVRYIVGDKHLYNSTHNEINIERSAIDLETVVMPAHVKKPLVDHIARYLRRK
                     ERSEWSRLDEFLEYGTALALFFQGPSGTGKTMMARALAHHFGRPLITVNLNNTQFSWT
                     LESVMIQAFREAALLRGFIFFDEADDIFKEGSSLARTLLIQIEKAKCVVIFATNKAAS
                     IDPAMERRLSMKIHFPLPDAEQRLKIWKALLPGFIKLAPDVNMKVLNERYPFSGGLIK
                     NTIFLAANSAEPDDRGNLLITGPMLTQAADLQTKQMAQDNKFCKLYRPERKITDLPLA
                     ERQRTELKNIARAFQYAKKKQSGLNLLISGASIETGISAAEALAAESGLSIKAFKFSE
                     MEGFIRENEIIDLVTQEKTKLMDYAFCRTTEEAHLLMIVDYNGIINWSDAGNQNDMDI
                     GLAAKSSVAALLNNLRDYRGLCCLVIHECPKAGIPLELQAHVKIDYPTEEMQMRQWEK
                     HLAPGTFTDDDLVELVQQHPMHIAEMEIILHRATVQALIEGRKPQPSLETIKAVIRRY
                     RGKNSVPVLFGRKA"
     gene            complement(2735..3301)
                     /locus_tag="PLR13_11490"
     CDS             complement(2735..3301)
                     /locus_tag="PLR13_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQC20006.1"
                     /translation="MAAKEKEREEKGLDDYGIIYLSGVIGEAKAEEICKQLIEYNIKG
                     SINQVQMVINSPGGTCTAGFSIIDIMEWSNIPIYTTGIGMIASMGLLIFMTGEKGHRV
                     VTPRTSILSHRFSAANFGNHSQLIAGRREEDMLHERILNHYMTYSAIKDRNELETHLL
                     RDVDTWLTTEEAIRMGIVDVVEPMRKRP"
     gene            3462..4484
                     /locus_tag="PLR13_11495"
     CDS             3462..4484
                     /locus_tag="PLR13_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQC20007.1"
                     /translation="MRLKLLKKLVDAITLLAQPTGTTISSLCERLGINKRQAYRVIHE
                     IQSEFKIMIDKDKPLLGGETRYYLDREFIRRLSEIKVADLNLTFQELLSLYSIKGHAR
                     LYRGTDIEANIERAFGKLDVFFPEAFSRKLEQVKALFLTADKLSKDYTGKEDIIEELA
                     EAVLQKKTCLVEYHSYSSGKVSSFHIDPLKLFEWNGGLYTSVRVSKYDAIRTLAVERI
                     NTLTVTDREFTYPEHYNPDEILEDAFGIIYDDPVTVKIRFCAKQAPYIRERKWCRNQQ
                     IEDLLDGCIILTLSTSGWYDVKKWILSFGADAELLEPADKRKEVLQEIQKAASLYRSG
                     TKPAGQ"
BASE COUNT         1053 a         1209 c         1172 g         1232 t
ORIGIN      
        1 cccatcgcaa caacgtccgg gttcgtcttg tagcatatcc ccctgttttt caccagccac
       61 gcattgtcat tgggtaaata gtaaggtttg tccggaacgc ccagtttgcc gacatcgtgg
      121 aacagtccaa cgatgacgca gctctcttcg ctgatcgaag gcgcgagaaa ctcccgaaat
      181 cgcaaaatgg tctccgccac gcctacgctg tgcttcagca aaccgcattc cacggccaga
      241 tggtaccttg tgcttgcggg tgaagttaac caggtggttt tgttttccag caaattaaca
      301 aattgatcaa atggttcctt acggtctgtg attttttgtt tcaatacatt atagcgatgc
      361 atcaattcca ttagtaacta cctcgttatg aactaatttg gctgtttttt aattcagtca
      421 cattacactc tgtgccaatc atgttggtca tgcttttttc tcatgctttt ctcccaaaca
      481 acaccggcac agagttcttt ccccgatacc gccttattac agccttgatc gtttccagtg
      541 agggctgggg ctttctgcct tcaatcagcg cctgaaccgt tgcgcggtgg aggatgattt
      601 ccatttccgc gatgtgcatg ggatgctgct ggaccagttc aaccagatcg tcatcggtga
      661 aggtgcccgg cgcaaggtgt ttttcccatt gccgcatctg catttcctcc gtcggataat
      721 cgatcttgac atgcgcttgc aattccaggg ggattccggc tttcggacac tcatggatga
      781 ccaggcagca aagaccccga taatcgcgca ggttgttcag cagcgctgca accgaacttt
      841 ttgcggcaag accgatgtcc atatcgtttt gatttccggc atcggaccag ttgatgattc
      901 cattgtaatc aacgatcatc agcaagtggg cttcttcggt tgtccggcaa aaggcgtaat
      961 ccatcagttt ggtcttttcc tgggtcacca gatcaatgat ttcattttcc ctgataaagc
     1021 cttccatttc gctaaattta aaagccttga tgcttagtcc cgattccgcg gcgagcgcct
     1081 ccgcggcgga aatgccggtt tcgatacttg caccgctgat cagaagattg agccccgact
     1141 gcttcttttt cgcgtattga aaagcccggg caatgttttt cagttccgtc ctctgtcttt
     1201 cggccagggg aaggtctgtg atttttcttt caggcctgta gagcttgcag aatttgttat
     1261 cctgggccat ctgctttgtc tgtaaatcgg ccgcctgcgt aagcattggc ccggtaatca
     1321 aaagattgcc ccgatcatcc ggttcagcgg aattggccgc caggaagatg gtgttcttga
     1381 tcaaaccgcc gctgaacgga taacgctcat tgaggacttt catattcaca tccggcgcca
     1441 gttttatgaa accgggcagc agcgctttcc agatcttcag tctctgttcc gcgtcgggca
     1501 gggggaaatg gattttcatg gaaaggcgcc gttccatggc ggggtcgatg cttgcggcct
     1561 tgttggtggc gaagatgacc acgcactttg ctttttcaat ctggatgaga agcgtccgag
     1621 ccagactgga gccttccttg aatatatcgt cggcttcgtc gaagaaaata aaaccccgca
     1681 gaagggccgc ttcccggaag gcctgaatca tgacggattc caatgtccaa ctgaactgtg
     1741 tattgttcag attcacggtg atcagcggcc tgccgaaatg atgcgccagc gccctggcca
     1801 tcatcgtttt tcctgtgccg gaaggcccct ggaaaaagag ggccagtgcc gtgccgtatt
     1861 ccagaaattc atccagccgg gaccattcac tcctttcttt ccggcggaga tacctggcaa
     1921 tgtggtcaac gagcggtttt ttcacgtgcg cgggcatgac gacggtttcc agatcaatgg
     1981 cgcttcgctc gatgttgatc tcgttatggg tgctgttgta caggtgcttg tcgccgacga
     2041 tatagcggac atggcgctcg ttgatggtta cggtttcatc catgacatgc gaacttctgc
     2101 attcatcatc gctcttgaag aaaataatct cgcgggcaac cagcggcgcg cctctgctga
     2161 aatgcctttt tttctccaat tgctccatgt agtccttaca gaggatgaaa agcatgacgc
     2221 ggatgataat gccgccggcg tcatcattta agccgcaggg gcaaaaagcg ttgcggaagg
     2281 tctcgctggt ggcctgcatg aacaggagca gaagaatttt ttcatcaaat tcatccagtt
     2341 ggttttcccg gcagaacagc ggaaagaaaa gcctgacgcc ggaccgtagc gttttgtttt
     2401 ggcgtgaccg gttgatatgc cgaacccggg caagctccgc ttcgatgtgt tcccaggtaa
     2461 tgttctgcgg cgtcaggccc agcccggcgc agcgccgaat cccttccccg tcgttttcca
     2521 gcccttcggc ttttcccgag cggaccgccg acaggacgag catcagcagg gcttcttttt
     2581 ccaatgcggc aaggtattca taattatcgg caaaggggct ttcctccagt gttgcctccc
     2641 cgtccggtac gatggcaagc cgctggccgg cctgtcctcc gggcggcgct ggttgatttc
     2701 tgacggcatt ttgcgtctga ttcatattcc ctccctaagg tctttttctc atcggttcga
     2761 ctacgtccac aatgcccatc cggattgcct cttccgtggt gagccaggta tcgacatctc
     2821 ttaaaaggtg tgtctccagc tcgttgcggt ccttgatggc ggaataggtc atgtaatggt
     2881 taaggattct ttcgtgcagc atgtcttcct cccgccgccc cgcgataagc tggctgtgat
     2941 tgccgaagtt tgcggccgag aagcggtgtg acaaaatgga cgtccggggc gttacgaccc
     3001 tgtggccttt ttctccggtc atgaaaatga gcaggcccat ggaggcaatc attccaatgc
     3061 ccgttgtgta tatgggaata tttgaccatt ccatgatgtc aatgattgaa aacccggctg
     3121 tgcacgtgcc gcccggtgag ttgatcacca tctggacctg gttgatgctg cctttgatgt
     3181 tgtattcgat gagctgtttg cagatttcct cggcctttgc ctctccgatc accccggaca
     3241 gataaatgat gccgtaatca tccagtccct tttcctccct ttccttttct tttgcggcca
     3301 ttctaaacct cccgtcttga ttttgacgtc atcatgccgc gagcgagtgt catttttggt
     3361 cattttgaaa aaaatatttt gcgtttgggg gcgattgccg gtaaggtgct tataattatt
     3421 gcgtttatcg cttcaaaaaa attgaccttc aggtgaaaat catgcgtctg aaactgctca
     3481 aaaagctggt tgacgccatt acgctgctgg cgcagccgac gggaacgact atttcgtctc
     3541 tttgcgaaag gctcggcatc aacaaaaggc aggcttaccg ggtgatccat gaaatccaga
     3601 gtgaattcaa gattatgatt gataaggaca agcccctgct cggcggcgaa acccggtact
     3661 acctggatcg agagttcatc cggcggcttt ccgaaattaa ggtggcggac ttgaacctga
     3721 cctttcagga actattgagc ctgtattcca ttaaaggaca tgcccgcctg tatcgcggca
     3781 cggacatcga ggcgaatatc gaaagggcct tcggcaagct ggatgtcttt ttcccggaag
     3841 ccttctcccg gaagctggaa caagtcaaag cgctgtttct gacagcggac aaactgtcca
     3901 aggattacac cgggaaggag gacattatcg aagaactggc cgaggccgtc ctgcaaaaga
     3961 aaacctgcct tgtggagtat cattcctatt cttccgggaa agtcagctcc ttccatatcg
     4021 atccgctgaa attatttgaa tggaacggcg gtctttacac ctcggtgcgc gtgtcgaaat
     4081 atgatgcgat tcgcacgctg gctgtggaaa gaatcaatac cctgacggtc acggacaggg
     4141 aatttaccta tccggaacat tacaatcccg acgaaatcct ggaagatgcc ttcggcatta
     4201 tctacgacga tccggtgacg gtgaaaatcc gcttctgcgc gaaacaggcg ccgtatatac
     4261 gggagcgcaa gtggtgcagg aatcagcaga tcgaagatct tctggacggc tgcatcatcc
     4321 tgaccctgtc cacctccggc tggtacgacg tcaaaaaatg gattctttcc ttcggagcgg
     4381 atgccgaact tttggagcct gccgacaaaa gaaaggaagt cctgcaagag atccagaagg
     4441 cggcctcgct ataccgatca ggcacaaaac cggcagggca atgaattgaa taattatgat
     4501 acgccccaaa actggaaaag caataaagtg cattttagtc agctaaaaac accgatacta
     4561 aacttcaaac ggtaatcatc aattataaaa accttccact gatgaacagt ggtaggtgct
     4621 atacgccctc cagcacaatt caaatttgct aagaatactt ttgcat
//
LOCUS       DAONQZ010000119         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_19502_length_4552_cov_9.680898, whole genome shotgun sequence.
ACCESSION   DAONQZ010000119 DAONQZ010000000
VERSION     DAONQZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19502_length_4552_cov_9.680898"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..4469)
                     /locus_tag="PLR13_11500"
     CDS             complement(306..4469)
                     /locus_tag="PLR13_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC20008.1"
                     /translation="MDQWTRVTKEKTVGQASPDSFVSAMNLGNELAKAGRYTEARDAF
                     SEAVRLNPQSSEAYVGRGNAKRAARDFAGAMSDYEAAVHLKPGDKDLLITLANSKTDM
                     RKYDEAIADLDKILGADPNNLDAIVSRGYARKQLKDTSGAKADFEKALALSPNHGFAS
                     RMLQSLGSSGQALAPQQTQMKPLPAPPDAGLPPVTAALPSVAGRSPAPAVSGQDGKPA
                     SPTPFPGASQPAAPGGAFQEFDPCKPDIHIGDLLWSNNEQPAQMKQKQARPDSRFNDL
                     LVFSSTRNNHPAAVAKAQLAYLMGDLSAADQVKFDEKWSYFFRYPSDESTVYFEKLIP
                     LLADLIEIRNMTTSAVLSRDEAMKDAEEAQAYENKEALANALMIARQQEEILRELDGE
                     LKTLAASIETLGDPPNPLANACTSRKKHEEAREVVKDTLKASIVPKQLKGETGVSYAF
                     KSELSGVPTGVKLHWDFGDGEVKEAPAGEVKHVFKKPGRFVITLSLFTKEKNEQIPLA
                     AAKAFIAGMDQNPQAAGQNDGKIEKTELLYKDSFTTPALPPGCRIQWNQGQKANDAVH
                     EDTRLKNPEKYPYYYLFQYKMSCPKTVLEEGVLNIPFHKTPQPFTVESSYGLIVDVKL
                     EYKPDHSQSELNKNLESFAKWKNEGAKLMKPFKFPGWEANGYIREDERVGGFTYMGLG
                     ENRTVLYVNYASPYVQFRSKASLEQCMSGDRRCNKEEYKIAEDFDREERARRIKKYTT
                     DLTKKAIEFVSAMRLKGLPYHYATVKSPIKMTRDDEEWLNEETEIISPDEFKKRQERL
                     AEAEKFYRSNIEILQKNLSREQADYSRETDPERKAAFFFRMMGIQADIQAEQDKINTL
                     HTGKIVHTRTQYDDYVQNKFIERIKEEQEKYVKLERAAAIIRKEAKKLPQGEREKAEE
                     FVDKHITGKTLATLDEATVKKAGEAIFSKTQGYNEAEAARYEEEAYMWDDYLTRAERV
                     KTAADIGMVGCSFLGGPMALSSAYDISTGYFQGGIKEAATQLVLQTGAFALGKYVGAG
                     SKAAVNADDAAAAAKTAARQSGKVSKGLSGAEELKIFQTNRARGEKTVKEFENLNQSL
                     KNAYLNKSPQNEIAAIKKQIREKTVEIQANPHAKNFLKYQGDKLTQTRFNRSIKSVHK
                     DVEKKFYEKMRKEPYKWSEFKIKEMRNASSGGSVGMDYDIGLIEKPNFIKLPDGTMKR
                     QYWLMRDGKPATRSQWQKEAQKCWNEAYEEVTLKNAERSWENLTTRIHPESYQDLAVL
                     DGNLNQVSRAWIQQTADVTRYKAMHMLKDGSMSKFEKLQEISRGTAKDLNTKLLPIFE
                     NMRKTPQVSAAAEHWKKVSSVLDDFGKNKIDPITAERRIAELTGGKSIPEVVDDMSNM
                     MESFVKLKK"
BASE COUNT          905 a         1224 c         1102 g         1321 t
ORIGIN      
        1 aggagcccaa gaatggcgta tttgatcgac atccgtcttt tcctctttgc gtggaccgaa
       61 gactatatat tttttatata cctgtcaaca ttttttttca taagatgaaa aatacccgaa
      121 tcaagacgcc aacaaaacgg caaaattccg gaaggctgac cgtgatgact gccgcgtgga
      181 aatccgaagc gcataaggga gtgataactt acagttacgc aatctacggc aattcgctgt
      241 tctctattta ttcttcggtt gcggatgaag ccgttcgcct gtcgatcgct ttacagttac
      301 cggatttact ttttcagctt tacaaaactc tccatcatat tggacatatc gtccaccact
      361 tcggggatgg atttgccgcc cgttaattcg gcaatgcgcc gctcggccgt aatgggatcg
      421 atcttgttct tcccgaaatc gtccagcacg gaagacacct tcttccagtg ttcggccgcc
      481 gcggaaacct gtggggtctt ccgcatattt tcgaaaatcg gcaatagttt ggtattgagg
      541 tctttggccg tcccgcggct gatttcctga agtttttcaa acttggacat ggagccgtcc
      601 ttgagcatat gcatcgcctt gtagcgcgtc acatcagccg tttgctgaat ccaggcgcgg
      661 gagacctgat tcaggtttcc gtccagcacg gccagatcct gataactttc gggatgaatc
      721 cgggtcgtca gattttccca ggaccgttcg gcatttttca gcgtcacctc ttcgtaggct
      781 tcattccagc atttctgcgc ttctttttgc cactggctgc gggtggcggg cttgccgtcc
      841 ctcatcagcc agtactggcg cttcatcgtg ccgtccggaa gtttgatgaa gttcggcttt
      901 tcgatcaatc caatgtcata gtccattccg acggaacctc cgctggaggc gtttctcatt
      961 tccttgattt taaactcact ccatttatac ggttcttttc tcatcttctc gtagaacttc
     1021 ttctccacat ccttatgaac acttttgata ctccggttaa aacgggtctg agtcagttta
     1081 tcgccctgat atttcaggaa attcttggca tggggattgg cctgaatttc gacggtcttt
     1141 tcccggattt gcttcttaat ggcggcgatc tcgttttgag gcgatttgtt taaataggca
     1201 tttttcagtg actgatttaa gttctcaaat tccttgacag ttttttcacc ccgcgcccgg
     1261 ttggtctgga agatcttaag ctcttccgcg ccggagagtc ctttagaaac ttttccggat
     1321 tgacgggctg ccgttttggc cgccgccgct gcgtcgtcgg cattgacggc cgctttggag
     1381 ccagccccca catattttcc caaggcaaaa gccccggttt gcaaaactaa ctgcgtggcc
     1441 gcctctttga tgcctccctg aaaataacct gtcgagatgt cataagccga tgacagcgcc
     1501 atcggacctc ccagaaagga acagcccacc atgccgatgt cggctgccgt tttcaccctt
     1561 tctgcccggg tcagataatc gtcccacatg taggcttctt cttcataccg ggcagcctcg
     1621 gcttcgttgt atccctgggt tttgctgaag attgcctctc ctgccttttt aacggttgcc
     1681 tcatcaagag tggccagggt tttgccggtg atgtgtttat cgacaaactc ttcagctttc
     1741 tctctttctc cctgcggcag ttttttcgct tccttacgga tgatggcggc ggcccgctcc
     1801 agctttacgt atttttcctg ttcttcttta atccgttcga tgaacttatt ctgcacataa
     1861 tcatcatatt gcgtccgggt atgaacgatt tttcccgtgt gcagagtgtt gatcttgtcc
     1921 tgctccgcct ggatgtcggc ctgaatgccc atcatgcgga aaaagaaagc cgcctttctt
     1981 tccggatccg tttcccggct gtagtccgcc tgttcccggg aaaggttttt ttgcagaatt
     2041 tcgatattgg agcggtaaaa tttttccgct tccgcgagac gttcctgtct cttcttgaat
     2101 tcatcggggc ttatgatctc cgtttcttcg tttagccatt cttcatcgtc cctggtcatc
     2161 ttgatcggac ttttgacagt ggcgtaatgg tatggcaatc ctttgagcct catagccgac
     2221 acaaactcga tggccttctt cgtcagatcc gttgtgtatt ttttgatccg tctggcccgt
     2281 tcctccctgt cgaaatcttc ggcgatctta tattcctctt tattgcaacg ccggtcgccg
     2341 ctcatgcatt gctcgagact tgctttgctg cggaactgca cataggggct tgcgtaatta
     2401 acatacagga cggtccgatt ctctcccagt cccatgtaag tgaagccgcc gacgcgttca
     2461 tcctcccgga tatagccatt cgcctcccag ccggggaatt taaagggctt catcaatttg
     2521 gccccttcat ttttccattt cgcgaaggat tcaagattct tgtttaactc ggactgagaa
     2581 tgatccggtt tatattccag cttgacatcg acaataaggc cgtaactgct ctctaccgta
     2641 aaaggttggg gggtcttatg aaacgggatg ttcagcacac cttcctcaag cactgtcttc
     2701 gggcatgaca ttttatattg aaataaataa tagtagggat atttttccgg atttttcaat
     2761 cttgtgtctt catgaaccgc atcatttgct ttttgtccct ggttccactg gatgcggcat
     2821 cccggaggaa gggctggcgt cgtaaaggaa tctttataga ggagctccgt tttttcgatc
     2881 ttcccgtcat tttgcccggc ggcttgcgga ttctggtcca taccggcaat aaacgcttta
     2941 gctgcggcca ggggaatctg ttcgttcttt tctttcgtaa aaagagacag cgttataaca
     3001 aagcggccag gctttttaaa gacatgcttc acttcccccg ccggtgcttc tttgacttct
     3061 ccgtcgccaa aatcccagtg cagtttcacg ccggtcggaa cgccggaaag ttccgacttg
     3121 aacgcataag atacgcccgt ttcccccttt agttgctttg gaacaataga cgctttcagt
     3181 gtatctttaa cgacttccct ggcttcttca tgtttctttc tggatgtgca ggcattggcc
     3241 aggggattgg gcggatcacc cagcgtttcg atgcttgccg ccagcgtctt taactctcca
     3301 tccaactccc gcagaatttc ctcctgctgc cgggcaatca tcaaagcatt ggccagcgcc
     3361 tccttatttt catacgcttg tgcttcttcg gcgtctttca tggcttcgtc tctgctcaga
     3421 acggcggacg tcgtcatgtt tctgatttca atcaagtcag ccagaagcgg aatcagcttt
     3481 tcgaaataga cggttgattc gtcgctggga taacggaaaa aatacgacca tttttcatcg
     3541 aatttcacct ggtcggccgc agaaaggtct cccatcaggt aggccagttg cgccttggcg
     3601 acggcggcgg gatgattgtt ccgcgtcgat gaaaatacga gaagatcgtt gaagcgggag
     3661 tcgggtcttg cctgcttttg tttcatttgc gccggttgct cattgtttga ccacagcaaa
     3721 tcgccgatat ggatatccgg cttgcagggg tcaaattcct gaaaggcgcc gccgggagca
     3781 gccggctgcg atgcacccgg gaaaggcgtc ggggatgcag gtttgccgtc ctgtccggaa
     3841 actgcgggag cgggggaccg gcctgcaaca gacggaagag cagccgtcac gggaggcaag
     3901 cctgcatccg gaggcgccgg gagcggtttc atttgcgtct gctgaggtgc aagcgcctgt
     3961 ccggatgatc ccagcgattg gagcatacgg gatgcaaaac cgtgattcgg gctgagggcc
     4021 agcgcttttt caaaatcggc ttttgcccct gaagtgtctt taagttgttt gcgggcgtat
     4081 cccctcgaca cgatggcgtc aagattattt gggtcggcac ccaaaatctt gtccagatcg
     4141 gcaatcgcct cgtcgtactt tcgcatgtcc gtcttactat tggcgagtgt gatcagaaga
     4201 tccttatcgc ccggtttcag atggaccgcg gcctcgtaat cgctcattgc gccggcgaaa
     4261 tctcttgcgg cacgttttgc attgcctctg ccgacgtagg cctcgctgct ttgcgggttt
     4321 agacgcacag cctccgaaaa agcgtccctc gcctccgtgt aacgaccggc ttttgcgagt
     4381 tcattgccca aattcatggc ggaaacaaag ctgtccgggg acgcctgccc aacagttttt
     4441 tccttcgtca ccctcgtcca ttgatccatc ccgaccctgt cgtccccttg gcaaaaaccc
     4501 gtcgcggcaa aaagaataaa caaactgaaa acaatgcccg cggcaattcg tt
//
LOCUS       DAONQZ010000120         4544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_19555_length_4544_cov_4.315661, whole genome shotgun sequence.
ACCESSION   DAONQZ010000120 DAONQZ010000000
VERSION     DAONQZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19555_length_4544_cov_4.315661"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLR13_11505"
     CDS             complement(<1..191)
                     /locus_tag="PLR13_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20009.1"
                     /translation="MITFNPDVTTHSKTRRDGEVCLNSVENVCLQATKPKGEKFSSKQ
                     NTGGGMRLQRGYWRRTPHLV"
     gene            complement(172..1167)
                     /locus_tag="PLR13_11510"
     CDS             complement(172..1167)
                     /locus_tag="PLR13_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HQC20010.1"
                     /translation="MALPAASYQPHRPQDSDYYHCVEDYFETFVQSYDEHFSKQYGFW
                     RPYVEQVIYRYLDCGDLHHGFARVRCKDCGHEYLLAFSCKRRHFYPSCHQKRVVEFGE
                     WLCMDVLKKAPHRHFIFSIPKILRRYFLYDRKLLAGLSRCAWESLKVFIQQAVPEKEP
                     LPGAVIAVQTFGDFLRFNPHCHILLTDGCFYGNKGMFRVAPPLEMKKLEAIFRHKVFK
                     MLLKKGKITAEMVRMLSAWKHSGFNIFCGNRISPKDETSMENLARYIIRASFSHLGLW
                     LFRSRPPAWARTADRPKICAATLSESTTSDSYEHPPHQHADCYADPDSWDDYIQS"
     gene            1260..1745
                     /locus_tag="PLR13_11515"
     CDS             1260..1745
                     /locus_tag="PLR13_11515"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC20011.1"
                     /translation="MREGPGVDLILNLHDIALEENTVGTVLCLDTLEHVEHPYRTIEE
                     IHRILKPNGMVIISSVMNYPIHDFPCDYWRFTPEAFRSILKPFPAVYVNYASEDNFPH
                     TVVGIGCKGTDIHFEDFEQAGNDWRHRWTEPEPPQNFTGKIKRETRRVYHQVSDILKH
                     K"
     gene            complement(1778..2593)
                     /locus_tag="PLR13_11520"
     CDS             complement(1778..2593)
                     /locus_tag="PLR13_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQC20012.1"
                     /translation="MSIKSYLYQSRPEIAVASEYYGAYRNFRKAGFKKTPFGFVMAGL
                     DSMQDGSYEHDETQKIIALIKEADVFVDVGANMGYYTCIARSMGLKILAVEPLWHNLQ
                     FLYGNLEANHWTDVEVFPLGLSNKPGLAVLYGVGTAASFIKDWADISTKKRMVPLSTL
                     DIIAANRFSGLRVVIKIDVEGVEYSVLQGAEMMLSCLPAPVWMMETGLTGNFPGGVNE
                     NFIKVFDLFWSKGYSAYDMMPEREDLVVEEQVRKWVKNSDTEGKVNFLFRKNG"
     gene            complement(2606..3643)
                     /locus_tag="PLR13_11525"
     CDS             complement(2606..3643)
                     /locus_tag="PLR13_11525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC20013.1"
                     /translation="MTFIDKTRTFLFVDDALKGGRNHHIDALKGMGIIMVIVCHTVSF
                     NDPAHFNVSLPFRVLDSFIMQLFFFLSGLVLFSQIKKYTLPAYLKKNAIRLLVPLLVW
                     SCIGYVLNQTYQQVNFPRFLLNLVMTPNLWFLWVLFLNSTILFSVLKLVEVRGWTRWE
                     NYFIIASILLIRAALSTNLFLLSEVRLYYPYYAAGFFAFKYYDELKARRNLLYAAAVA
                     LYPVLAVCYIGYAFPIFYPYLLQFTDEKISRLIVSVYKYVVAFSGIAFWSFILERLRQ
                     TRLYLFLCWTGTISLEIFVCHGHFIARLGTSGLLYAASTLIALVGSIALTVLVIKRFR
                     ITRLLLAGQNR"
     gene            4145..4501
                     /locus_tag="PLR13_11530"
     CDS             4145..4501
                     /locus_tag="PLR13_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQC20014.1"
                     /translation="MKHRRSFAADLKRQIIEELLSGVSTPAQLIRRYEISSSLLYHWK
                     EQYAKGRFNNEPSKEAALEDRVRQLEQLVGKLTMENEFLKKAVQRGLLTLPKKNDNSL
                     LNTVTSLKTRKGGANL"
BASE COUNT         1284 a         1081 c         1088 g         1091 t
ORIGIN      
        1 acaagatgtg gtgtgcggcg ccaataaccg cgctgaaggc gcattccgcc ccccgtattt
       61 tgttttgaag aaaatttttc accttttggt tttgttgcct gcaaacagac attttccaca
      121 gaattcaggc agacctcgcc gtcacgtcgt gtttttgaat gcgtcgtgac gtcaggattg
      181 aatgtaatca tcccatgagt cagggtcagc gtaacagtcg gcgtgttgat gtggaggatg
      241 ctcgtaagaa tcggaggttg tggattcgga taatgtggcg gcacagattt tcggtctgtc
      301 tgccgtgcgg gcccaggcag gcggccttga tctgaaaagc caaagcccca aatgcgagaa
      361 cgacgcccgg attatgtaac gcgccaaatt ctccatcgac gtttcatctt tcggtgatat
      421 ccggttgccg cagaagatgt tgaagccgga atgtttccat gcggaaagca tccgcaccat
      481 ttctgcggtg atcttgcctt ttttcaggag catcttgaat actttgtgcc ggaaaatggc
      541 ttccagcttt ttcatttcca gcggaggggc gacgcgaaac atgcctttgt ttccgtaaaa
      601 gcagccgtcc gtcaggagaa tgtggcagtg gggattgaat cttaaaaaat caccgaaggt
      661 ctggacggca atgaccgcac cgggcagggg ttccttttcc ggcacggcct gctggataaa
      721 aaccttcagg gattcccagg cgcagcggct gagaccggca agaagctttc tgtcatacaa
      781 gaagtaacgg cgcagaatct tcgggatgct gaaaatgaaa tgacgatgcg gcgccttttt
      841 gaggacatcc atgcagagcc attcgccaaa ctccacgacg cgcttctgat gacaggacgg
      901 gtagaagtgt cttcttttac aagaaaacgc cagcaggtat tcatgcccgc agtctttgca
      961 tcggacacgg gcaaagccat gatgcaaatc tccgcaatca agataacgat aaatcacctg
     1021 ctcgacataa ggccgccaga agccgtactg tttggagaag tgttcgtcgt aggactggac
     1081 gaaggtttca aaataatcct ccacgcagtg gtaataatcc gaatcttgcg ggcggtgggg
     1141 ctggtatgag gctgccggta atgccatgtg acctttgcac aactcgaaag attattgaat
     1201 ttcattgccc aaacttactt tagattcctg ttgtaactct gtggttaatc ggttgcgaca
     1261 tgcgagaagg acccggcgtc gatctcatat taaatctgca tgacatcgca ctcgaagaaa
     1321 atacggtcgg caccgtcctt tgcctagaca ccctcgaaca tgtggaacac ccctacagga
     1381 ccatagaaga aattcatcgg atattgaaac cgaacggaat ggtgatcatc agctccgtca
     1441 tgaattatcc catacacgat tttccctgcg actactggag gtttacgccc gaagcgtttc
     1501 ggtccatatt gaagcccttt cctgctgtct acgtaaacta tgcaagtgag gataatttcc
     1561 cgcacacggt tgttggcatt ggctgcaaag gaacagacat tcattttgag gattttgaac
     1621 aggcaggaaa cgattggcgg cacaggtgga cggaacccga accgccccaa aattttacag
     1681 gcaagatcaa aagagaaacg aggagagttt atcaccaggt ttcagacatt ttaaagcaca
     1741 aatgaaaatc agccttaaat aactcctttc atggaattta accgtttttc cgaaacagaa
     1801 aattgacttt gccttccgta tcggagtttt ttacccattt gcgtacctgc tcttccacaa
     1861 ccagatcttc ccgctccggc atcatgtcat aggccgagta tccctttgac caaaacaggt
     1921 cgaagacttt aataaaattc tcattgacac cgccggggaa atttccggta agacctgttt
     1981 ccatcatcca caccggcgcg ggcagacacg aaagcatcat ttccgcaccc tgaaggacgc
     2041 tgtattccac accttcaaca tcaattttaa tgaccactct caagccggaa aaacggtttg
     2101 ccgcaataat gtccagtgtg ctcaacggca ccatacgttt ttttgtagat atgtcggccc
     2161 aatccttgat gaaggacgcg gccgtgccca cgccataaag aacggccaga cctggtttgt
     2221 tcgacagtcc cagggggaag acttccacat cggtccagtg attcgcctca aggtttccat
     2281 agaggaactg cagattgtgc cataacggtt caacggcaag gattttcagg cccattgacc
     2341 gcgcaatgca ggtgtaatat cccatgttcg cccctacatc gacaaacaca tccgcctctt
     2401 tgataagggc aatgattttt tgtgtttcat cgtgttcgta agaaccatcc tgcatggaat
     2461 caagaccggc catcacaaat ccaaaagggg tcttcttgaa gcccgccttg cgaaaatttc
     2521 ggtaagcgcc gtaatattca ctggccacgg cgatctcggg acggctttga tagagatatg
     2581 atttaatact cataatactc cttctttatc gattctgacc cgccaaaagc agtcgggtaa
     2641 ttctaaaacg cttgatgacc agcacagtca gcgcgatgct cccgacaagc gcgatcaatg
     2701 tactggctgc ataaagcaat ccgctggtgc cgagcctggc aataaaatgg ccatggcaaa
     2761 caaaaatttc cagggatatg gtccccgtcc agcaaagaaa aaggtaaagc ctggtctgcc
     2821 gcagtctctc cagaataaag gaccagaaag ctattcccga aaaggccacg acatatttat
     2881 agacggaaac aatcaatcgg gaaatctttt catctgtaaa ctgaagaaga tacggataaa
     2941 agatcggaaa cgcgtatcct atgtaacaaa ccgccagaac cggatagaga gcgacggccg
     3001 cggcatacag aagattccga cgggctttga gttcatcata atacttgaag gcaaaaaatc
     3061 ccgccgcata atacgggtaa tagagcctga cttcagacaa aaggaacaga ttggtggaaa
     3121 gggctgctct gatcagcaaa attgacgcga tgataaaata attctcccaa cgggtccatc
     3181 cgcggacctc aaccagcttg agtacggaga agagaatcgt gctgttcaaa aaaagaaccc
     3241 acaggaacca tagattcggt gtcatcacta gattcagcag gaaccgggga aaattcacct
     3301 gctgataggt ttgattgagc acgtagccga tgcacgacca gaccaaaaga ggcaccagca
     3361 gtcggatggc gttctttttc agataagccg gcagagtata tttctttatc tgcgaaaaca
     3421 gaaccaggcc gctcaaaaag aagaacagtt gcatgataaa cgaatcaaga acacgaaagg
     3481 gcagagaaac gttgaaatgg gccgggtcat tgaacgaaac ggtatggcaa acgatgacca
     3541 tgataatccc catgcccttg agcgcgtcga tgtgatgatt cctgccgcct ttcagtgcgt
     3601 catccacgaa aaggaaggtt ctggttttgt ctatgaaagt catggggttt gcccttttgc
     3661 ccgatcccga taaagctcac tggtgtcggt cttgataaca tatcggtttc ttttttaaaa
     3721 catgtttttt atttttatgt gctaaagcct gttgcgaaac atgcattgaa ttgatattga
     3781 ttgataagag gttgctttta gttgtcggca gtataagaaa cgttctcttg tgtgtcaaga
     3841 atattttgcc acgggtaatg tatcatatct tgtgtcaaag aagggaaaga ggcgcccaca
     3901 gtaatagatt tgcccaaatc taaataaagg aggcgcccca tgtcccggaa ctgcataagc
     3961 agtttgccac aagcgtttgc tgtaatcaag gaaatgaatc tttctccgga atgggactcg
     4021 gattatcgat tggctgttcg gaaggctctt gttcatattg gactgcaccc ctcaagttgg
     4081 acaaaatatg ttagagtagt tgacaggaac ccaagtcccg ggaaacggga gaaaggatct
     4141 tgccatgaag caccgaagaa gttttgctgc cgacctcaaa cgccagatta tcgaagaact
     4201 gctcagtggc gtcagtacgc cagcccaact catacgccgt tatgagattt cctcgagcct
     4261 tctttatcac tggaaagagc aatatgccaa gggtcggttt aacaatgaac caagcaaaga
     4321 ggccgctttg gaagaccgcg tccgccaatt ggaacagctg gtcggtaaac taaccatgga
     4381 gaacgagttt ttaaaaaaag ccgttcagag gggccttttg acactaccga agaaaaacga
     4441 caattcatta ttgaacaccg tcacctcctt gaaaacacgg aaagggggtg ccaacttatg
     4501 aatctgcccc gcagcacata ttatcatcaa tcaaagagca ataa
//
LOCUS       DAONQZ010000121         4365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_20514_length_4365_cov_18.736891, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000121 DAONQZ010000000
VERSION     DAONQZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20514_length_4365_cov_18.736891"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLR13_11535"
     CDS             <1..340
                     /locus_tag="PLR13_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20015.1"
                     /translation="EKDNVVQGMLDDEYVRCLDVIKALQAKMESYPKGALNIRRKKIK
                     DKEYIYYYLVWREGQDVINRHVAADNLPELQKQIADRDKLRNEILAYKERLAYLKKLL
                     NKSVPGKKHD"
     gene            466..1011
                     /locus_tag="PLR13_11540"
     CDS             466..1011
                     /locus_tag="PLR13_11540"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HQC20016.1"
                     /translation="MASGMFCEPWTLILLYTLLIQKIASRVDLNQCITDLGFVDYFTA
                     DGMQRFTGGGYEVEFIGQRAGGRDKGFLSVPEWHLTAMPLPFINILIDYSTTSMLCGF
                     SIRFPVPEAFFLHKLITAQKRLSDTKRMKDIEQCSVIAKIINDDRLQQIIHNQRFSKA
                     TKKYIALSCAAIEFPLHRIGM"
     gene            1185..3410
                     /locus_tag="PLR13_11545"
     CDS             1185..3410
                     /locus_tag="PLR13_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC20017.1"
                     /translation="MLKKRRRHVHHTQPVHGNLDKNERFIKRQCCIYLERLLAVFPLT
                     DRDTVEMLTYVLGPKISELKKTISKKVYSKQRHTPVFDTDDELDILDDDSDDFYQMIK
                     HAGKKIQKEFITLSRTVLAKRVKTLSYAGKSEMEKKLDTLDKMFALSNEEKAFTTLLY
                     INTVWPRADEYFVDHLHCQDIFGRRYLKVMLQITDSGINRILSGTLARIEFFEFSNHS
                     FNLSDDYLGFFQKPLKDVLKKKDFVRFSGKTIPLKNHLIDPGVTKHILSLLRTKRESS
                     NHILLYGPPGTGKTSYALGLVNKLKIPAYAIMDDSCNQTRTRRAAIIACRNMTNDGEG
                     SLIIVDEADSLLNTEESWSSGGEIKDKGWLNQLLEEPGARMIWITNSIEDMEDSVLRR
                     FAFSVHFPAFNRRQRFSLWESVLCRHHVRKLFKDDEINKLTARYPVSAAIINMAVTKA
                     LESAAADHIAFKEIMIMNLDAHWKLLNGGAKPKTGDRIEEHYSIEGLNIEGNLPVILA
                     QLDSFDRCLRAPDKSGVRNFNLLFYGPPGAGKSELARYVAKRLDRELMVKRASDIVSP
                     YVGETEQNISRVFSEAESMEAVLVIDEADSFLFSRDRAVRSWEISHTNEFLTQMERFR
                     GILICTTNRFADLDMASVRRFNQKIGFGCLKPAENMIFYQKLLCGLCKTPVNRNQEEL
                     IGRIHDLTPGDFRIVRDRFAISDEKCVTHEQMIQALEAEARVKKTQQGNRPIGFMRRT
                     A"
     gene            3472..4224
                     /locus_tag="PLR13_11550"
     CDS             3472..4224
                     /locus_tag="PLR13_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20018.1"
                     /translation="MEEGKHFIGVGDTGHRAAKELCRIYEEMRGCLFESEQDRPHNHL
                     RIHSYPCAETACFDDKDHLVILVGSLIDPGLQDARTSFHESRPYFMLTVGIDHQTRVD
                     ADDIQPLPDECLIYPDTSLSVPVKLAQLVLQIFLIHTPWYISKRGALIGYDLYDTKQL
                     FGGKVVKAISMTSDRAHYRQNFSMFLDKNRADIRRTKGILMSVWGLSIMQANEILKET
                     RLLLTAGADELFTCHNLPADEPNILVTLFLAF"
BASE COUNT         1239 a          945 c         1053 g         1128 t
ORIGIN      
        1 ggaaaaagac aatgtcgttc agggtatgct ggatgatgaa tatgtgcgct gtctggacgt
       61 tatcaaggcg ctccaggcaa agatggaatc gtatccaaaa ggcgccctca atatccgccg
      121 gaaaaaaata aaggataaag aatacattta ttattatctg gtctggcggg aaggacagga
      181 tgttatcaat cggcatgttg ccgcggataa cctgcccgaa cttcaaaaac agattgctga
      241 tcgcgataaa cttcgaaatg aaattcttgc ttataaagag cgtcttgcct atctgaaaaa
      301 gctgctgaat aaatccgttc cggggaagaa gcatgactaa aagccttccc gataatctct
      361 ttcccgaaag gatcaggaat cttatttgcg cgataagcaa agccggattc tttgatcggt
      421 ctgctttgat cggaagctgg ttaatgccga tatatcagga actctatggc atcaggtatg
      481 ttttgcgaac cttggacatt gattttgctg tacacattgc tcatacaaaa aatagcatcg
      541 cgggtcgatc tcaatcaatg catcaccgat ttaggatttg tggactattt caccgctgac
      601 ggaatgcaaa gatttaccgg cggcggttac gaggtggaat ttatcggaca aagagccgga
      661 ggtcgagata aaggcttcct ttccgtgccg gaatggcacc tgacggccat gccgcttcca
      721 ttcatcaata ttctaattga ttattcgaca acgtcgatgc tttgtggctt ttcaattcgc
      781 ttccctgtgc ccgaagcatt ctttcttcat aagcttatca cggcccagaa acgtttatcc
      841 gacacaaaac gaatgaagga tattgaacaa tgttcagtca ttgcaaagat tatcaatgat
      901 gaccgattgc aacagatcat ccacaaccag cgcttcagca aagcgactaa aaaatatatt
      961 gcactatcat gcgccgctat cgaatttccg ctacatcgaa ttggaatgta accggcagta
     1021 gtggaaatta tcaagtcata tcatttctct atccagcctt cttttttaaa taaataataa
     1081 cgattcgtgc cgcaaaatgg aacgatccgg tgttatgatt tatccaaaag cacgaacctc
     1141 ctttgtgggc ttggtgcgga aaatcggaaa ggataaatca tacgatgctc aagaaaagaa
     1201 gacggcatgt ccatcacacg caaccggttc atggcaatct cgacaaaaat gagcgtttta
     1261 tcaagcgcca atgttgcatt tatctggagc gcctgctggc cgtctttcca ttgacggaca
     1321 gagatacggt ggagatgtta acatatgttc tggggccgaa aatatccgag cttaagaaaa
     1381 ctatcagtaa aaaagtttat tccaagcaac gtcacacacc agtttttgac acggatgatg
     1441 aactggatat cctggatgat gattccgacg acttctacca gatgatcaag cacgcgggaa
     1501 agaaaattca aaaagaattc atcacactca gtcgaaccgt tctggcgaaa cgtgtcaaaa
     1561 cgctatctta tgcaggcaaa agcgaaatgg aaaagaaact cgatactttg gataaaatgt
     1621 tcgctttgtc gaatgaggaa aaggccttta ctacattgct ttacatcaat acggtctggc
     1681 cgcgtgccga cgagtatttt gtggatcatt tgcactgcca ggatattttc ggcaggcgtt
     1741 atctgaaggt gatgctgcag attaccgact cgggaatcaa ccggattctg tccggcacgc
     1801 tggcgcgtat tgaattcttt gaatttagta atcattcttt caatctgagc gatgattatt
     1861 tgggattttt tcagaaacca ttaaaagatg tgctgaaaaa gaaggatttt gtccgctttt
     1921 cgggcaaaac cattcctctg aagaatcacc tgattgatcc cggcgttacc aaacatatcc
     1981 ttagccttct cagaaccaaa cgggaatcat caaaccatat cctgctttat ggtcctccgg
     2041 gcacggggaa aacaagttat gctctgggtt tggtcaataa attaaaaatt cccgcctacg
     2101 ccattatgga cgattcatgt aatcagacac ggacgcgcag ggcggcgatt attgcctgcc
     2161 ggaatatgac gaacgatggc gaaggatctt taatcatcgt tgatgaagcg gacagtctgc
     2221 tcaataccga ggagtcatgg agctccggcg gcgaaataaa ggacaagggc tggcttaatc
     2281 aactgctgga agagccgggt gcccgaatga tctggattac gaacagcatt gaagatatgg
     2341 aagattccgt actgcgccgt tttgccttta gtgtccattt tccggcattc aaccggagac
     2401 agagattctc gttgtgggaa tcagtgcttt gtcgccacca tgtccgaaaa cttttcaaag
     2461 atgatgagat caacaaactg acggcgcgct atccggtcag cgcggccatc atcaatatgg
     2521 ccgttaccaa agcgctggaa tcagcagcgg ctgatcacat tgcctttaaa gaaatcatga
     2581 tcatgaatct ggatgctcat tggaagcttt taaacggcgg cgcaaagccc aaaaccggcg
     2641 accgcattga agaacattat tccatcgaag gtttgaacat cgaaggaaat ctgccggtta
     2701 tcctggcgca gctggactct tttgaccgct gcctgcgtgc gcctgataag agcggcgtcc
     2761 gcaacttcaa tctccttttt tacggaccgc ccggcgcggg aaaaagcgaa ttggcccgct
     2821 atgtcgccaa gcgtctggac agagagttaa tggttaagcg cgccagtgat attgtcagcc
     2881 cttatgtagg cgaaacagag cagaatatca gccgggtgtt ttccgaggcg gaatcaatgg
     2941 aagcggtgct ggtgattgac gaggcggatt ctttcctttt cagccgcgac cgcgctgttc
     3001 gctcctggga aatcagccat accaacgagt ttttgacgca gatggaaaga ttccggggca
     3061 ttttgatctg cacaaccaac cgctttgccg atctggatat ggcttccgtc cgccgtttta
     3121 atcaaaaaat cggatttggc tgtctgaaac ctgcggagaa tatgatcttc taccaaaaat
     3181 tgctttgcgg attatgcaaa acaccagtga acaggaacca ggaagagctg attggacgta
     3241 tccatgatct gacgccggga gacttccgta ttgtgcgaga ccgttttgcg atcagtgatg
     3301 aaaaatgtgt gacgcacgag caaatgatcc aggcgctgga ggcagaagct cgcgtcaaaa
     3361 aaacacaaca aggcaacagg ccgattggtt ttatgcggcg taccgcatag gcgaatacaa
     3421 agaagtcaat caacaatcag ttttatagtt cttaatcatg cgggattatt gatggaagaa
     3481 ggcaagcatt ttatcggtgt gggtgacacc ggacacagag cggcaaagga attatgtcgg
     3541 atttacgagg aaatgcgggg atgtcttttt gaatcagaac aggacaggcc acacaaccat
     3601 ttgcgtattc attcctatcc ctgtgccgaa acggcctgtt ttgatgataa agatcatctt
     3661 gtcattctag tggggtcgct catcgatccg ggtttgcagg atgcgcggac gtcttttcat
     3721 gagagcaggc catattttat gttgacggtt ggtattgatc atcagacaag agtggacgct
     3781 gacgacattc agccactacc tgatgaatgt ttgatttatc ctgacacgtc tctttctgtt
     3841 cctgtcaaac tggcgcaact ggttttgcaa atctttttga tccacacgcc atggtacata
     3901 agcaaaagag gtgcgctgat cggttatgat ctttacgaca caaagcagtt atttggcggc
     3961 aaagttgtaa aagccatatc catgacatct gatagggcac attaccggca aaacttctcc
     4021 atgtttcttg acaaaaatag agccgacatc aggcggacaa agggcatcct gatgtcggtc
     4081 tgggggctgt ctataatgca ggcaaatgag attttgaagg aaacaagatt gctgctgacg
     4141 gctggggcag atgaattgtt tacgtgtcat aatctgccag cggatgagcc gaatattttg
     4201 gtaacgctgt ttcttgcatt ttaaaacgat tttttcgggg tactgtactg atgacaacgg
     4261 aaatgatgac agagccaatc ataaaatatt gccgggacaa gattcaacgg cggttgaatt
     4321 taagcattac gccggacata cagatacgga ttagaaattt tgatg
//
LOCUS       DAONQZ010000122         4334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_20713_length_4334_cov_25.513671, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000122 DAONQZ010000000
VERSION     DAONQZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20713_length_4334_cov_25.513671"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PLR13_11555"
     CDS             <1..1839
                     /locus_tag="PLR13_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQC20019.1"
                     /translation="IASIGVVFGDIGTSPLYAIKACFHGKHAIVPSAMNILGVLSLIF
                     WSITVVVSVKYLTFILRADNRGEGGIFALVSLLNTSEKKLSRFIQAVLLFAAILGAGL
                     LYGDGVITPAISVLSAIEGLEVATKAATPFIVPLTCIVLVLLFSLQQKGTANIGKLFA
                     PIMVLWFLSIGFFGLMQVTREPTVLLALNPVYALEFFMNNGMHGIVVLGSVVLCITGC
                     EALYADLGHFGRNAIRLSWFSFVFPSLLCNYFGQGALLLAHPEMNINPFYKLVPESFL
                     YPMIGLSTAATIIASQALISGTFSLTQQAVQLGLFPRVRIIHTSSDMQGQIYIPFVNY
                     ALMLACIGVVVGFKESSSLAGAYGIAVTGTMIITSLLFFLVLMHYRRWPLWKVIPLVG
                     IFIAFDVAFFVGNTFKIIDGGWFPLFVAAIVALVMTTWKKGREELYRNLIDARLPIES
                     FLADLPRSHIPRVSGTAVFMTLSPLGTPRTLLHNVKHNHVLHEQVVFLSIMAKDAPIV
                     PAGERIKIQDLGQGFYRVEAFYGFMQKPNVPQIMKIVAQYGLITDPMTTTFYLGRETL
                     MTSGKSKMMRWRKSLFAVMSRNAGNPTSYFGIPANRVVELGAQVEL"
     gene            complement(1927..2427)
                     /locus_tag="PLR13_11560"
     CDS             complement(1927..2427)
                     /locus_tag="PLR13_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20020.1"
                     /translation="MQRRTGVTAVGPSALRGQGKGVLKATQSYLGAMDLSRIPSSNQA
                     KYTAWLDQHTNALLEKLPIRNKPWGAARKALNLFMRDTLYNRYLNDYYKIDRLEFLLE
                     VPLDSAVAKGLKHNAGHGQLPQWPGLKNLKPNVSSIFQKYASVHAKNKNISRVHLDVY
                     LWLENR"
     assembly_gap    2472..2571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2626..4296)
                     /locus_tag="PLR13_11565"
     CDS             complement(2626..4296)
                     /locus_tag="PLR13_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC20021.1"
                     /translation="MSFNTIGDAVKSQSREVKPAVSEVAIFKHLIKERVHALDLIREL
                     LSNAGAEQVGAKRIEISYTQDKDGHIFEIVDDGCGMNYTSNDSIPGRLDRFLGLGLSG
                     IIGLDADEFSWKGLGSKLSYQSRKVEIETCAGDPHGLYVVLVNEPWESLNNNKVPRPR
                     INEHPSSHKGTKIKVTGHPPHRKEAPFTFDQLKNYLLHRTFAGYTSKRKYEPEILLSV
                     LGRTEIIPFGFPEFKSIDFDSFEHEGVKLDVATQTLFINVTPKSSKSMRVRLKGFVTW
                     NASHYDLSKNNLNTGLILSVKGIPYFTLNMEEYGATSIRTARPGEERTCLVVECDGIQ
                     DEMNISRSALVDSANTVELKKIVTELFQRVESSDVYLSFRTLPERNKVEKQGGILAEE
                     KRKIEQPDQNWVVYEKASDVYTILIREPQNEQEVNALIWKLEALGALPFENFNTLAYI
                     GASKGPDLLANFQEDKASEPFRATIIEIENNFYNYKTHGHTPTQYPKVLCWDIPASGR
                     KAKINKTQKPYKFTLSTDEYQVHIYVIKYMDGIKVLSRTDLEKRGIKI"
BASE COUNT         1064 a          876 c          890 g         1404 t
ORIGIN      
        1 atcgcttcta ttggtgttgt tttcggcgat atagggacta gtccgcttta cgccattaaa
       61 gcatgttttc atggcaagca cgccattgtc cccagcgcga tgaatatttt gggagttctg
      121 tctcttattt tctggtcgat taccgttgtc gttagtgtca agtatttaac ttttattctt
      181 cgggcagata atcgcggtga aggcggtatc tttgcgctgg tcagtctttt gaatacttcg
      241 gagaaaaagc tgtcccgctt tattcaggcc gttcttttgt ttgccgcaat tctgggagcg
      301 gggcttcttt acggagacgg tgttattact ccggcgatat ctgtcctttc agcaattgaa
      361 ggactcgaag tcgccaccaa agccgcaacg ccatttatag tgcctttgac ctgcatcgtg
      421 cttgttttgc ttttttcact acagcaaaaa ggtactgcta atatcggaaa attatttgcg
      481 ccgatcatgg tgctttggtt tttgtccatt gggttttttg gattaatgca ggttacgcgt
      541 gaacccacag ttcttctcgc gcttaatccg gtatatgctc tggaattttt tatgaataat
      601 ggtatgcacg gtattgttgt gctcggatct gttgtgctgt gtattaccgg ttgtgaagcg
      661 ctatatgctg atttggggca tttcggcaga aacgccattc gcttgtcctg gttttctttt
      721 gttttccctt cgttgctatg caactatttc gggcaagggg ctctgttgct tgcgcatccc
      781 gaaatgaata tcaatccttt ttataaactc gtaccggaat catttttata cccgatgatc
      841 ggactttcca cggctgccac aataatagct tctcaggcat taatatcagg cactttttca
      901 ctcacacagc aggccgtgca gctgggctta tttccacgtg ttcggattat tcatacctca
      961 agcgatatgc aggggcaaat atatattcct tttgtaaatt acgcgttgat gcttgcctgt
     1021 attggcgtgg ttgtcgggtt taaagaatcg agttcattgg ctggcgcgta tggaattgcc
     1081 gtgacaggga ccatgatcat tacatctttg cttttctttc ttgttttgat gcattaccgg
     1141 agatggccgc tctggaaagt aatacccctg gtcggaatat ttattgcgtt tgatgtcgcg
     1201 ttttttgttg gcaatacttt taaaattatt gacggaggat ggtttcccct ctttgttgca
     1261 gccatcgtag cgctggtcat gaccacgtgg aaaaaaggca gggaagaact ataccgcaat
     1321 ttgatagacg caagattgcc catagagagt tttcttgctg acttgccaag aagccatata
     1381 ccgcgcgtct ccggcacagc tgtttttatg actttatcgc ctctgggaac gccgcggaca
     1441 ctgcttcata atgtaaaaca taatcatgtg ttgcatgaac aggttgtgtt tctttccatt
     1501 atggcgaagg atgccccgat tgttcccgcg ggcgaaagaa tcaaaattca ggatttgggc
     1561 cagggctttt accgtgttga ggccttttat ggttttatgc agaaacctaa tgtgccgcag
     1621 attatgaaaa ttgtcgccca gtacggatta attacggatc cgatgacgac aacattttac
     1681 ctggggcggg aaacgcttat gaccagcgga aaatcaaaga tgatgaggtg gcgtaaatca
     1741 ttattcgccg tgatgtccag aaatgcggga aatccaacgt cctatttcgg aattcctgcc
     1801 aatcgggttg ttgaacttgg cgcgcaagta gaattatagt tatttagtct gagtttgatt
     1861 tatctgtaaa gataacgaaa agctaagccg gagccgcgaa gcggcgatcg gctggttggc
     1921 ctttccttat ctgttttcca accatagata gacatcaaga tgcacacgtg aaatattttt
     1981 attctttgca tgcacacttg catacttttg aaaaattgag cttacatttg gttttaaatt
     2041 tttaagcccc ggccactgcg gtaattgacc atgcccagca ttatgtttga ggcctttagc
     2101 tacagcacta tccagaggta cttccaacaa aaattcaagt ctatcaattt tgtagtaatc
     2161 attgagataa cgattataaa gtgtatctcg cataaatagg tttaaggcct ttcgcgccgc
     2221 tccccaaggt ttattgcgga tgggtagttt ttcaagtaat gcgtttgtat gctgatctaa
     2281 ccacgctgta tatttagcct gatttgatga aggaattctt gataagtcca ttgctccaag
     2341 atacgattgg gttgctttta atacgccttt cccttgccct cgcaatgcag aaggaccaac
     2401 ggccgtcacg cctgttctgc gctgcatagt atctatgaaa tcatttgtct tcataattca
     2461 taggccaacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacgacaac
     2581 atcagccgga gcgaagcgat cggctggatg tattggttgt gctttttata tttttatacc
     2641 tcgtttttct aaatcagttc ttgaaagaac ttttatacca tccatatatt taatgacata
     2701 tatgtgaact tggtattcat ctgtgcttaa tgtgaatttg taaggttttt gagttttatt
     2761 tatttttgct tttcttccgc tagcgggaat atcccaacaa agcactttgg gatattgagt
     2821 tggtgtgtgt ccgtgtgttt tgtaattgta aaagttattt tctatttcaa ttattgtcgc
     2881 tctaaatggt tcacttgctt tatcttcctg aaaattggct agaagatctg gcccttttga
     2941 tgcaccgatg tatgctaaag tattaaaatt ctcaaaagga agagcaccaa gagcttctaa
     3001 tttccatatt aaagcattga cctcctgttc attctgcggt tcacggatta gtatggtata
     3061 gacatccgat gctttttcat aaacaaccca attttgatcc ggttgctcta ttttgcgctt
     3121 ctcttccgcc agaatgcctc cttgcttctc aaccttattc cgttccggca aagttctaaa
     3181 agaaagatat acatctgatg attcaacgcg ctgaaacaat tccgtgacaa tttttttaag
     3241 ttctactgta ttcgcagaat caacaagggc acttcttgaa atattcattt cgtcttggat
     3301 tccatcacat tccacaacaa ggcaagttct ttcttcaccg ggtcgcgccg ttcggattga
     3361 tgtggctcca tactcttcca tattcaatgt aaaatagggt attcctttaa cagatagtat
     3421 aaggcctgta ttcaaattgt ttttggaaag atcataatga gatgcattcc acgtcacaaa
     3481 gccttttaat ctaacacgca ttgactttga acttttaggg gtaacgttta taaaaagtgt
     3541 ttgtgttgca acgtcaagct tcacgccttc gtgttcgaaa ctatcaaagt caatgctttt
     3601 aaactcagga aatccaaaag ggataatttc ggtacgtcca agaacggata ataatatttc
     3661 gggttcatat ttacgctttg atgtgtatcc agcaaaagtt ctgtgaagca aatagttttt
     3721 aagttgatca aaagtgaacg gggcctcctt acgatgtgga ggatgtccag taactttaat
     3781 ttttgttccc ttgtgtgaag agggatgttc gtttattcgg ggacgaggta ctttgttatt
     3841 atttagagat tcccaaggtt cattaaccag gacaacgtaa agaccatgcg gatcaccagc
     3901 gcaagtctct atttcaactt tacgggattg atatgatagt tttgagccaa ggcctttcca
     3961 agaaaactca tctgcatcaa gaccaataat gccagacagg cctaatccta aaaatctatc
     4021 tagtcgacca ggtattgagt cattgctggt ataattcatg ccacaaccat catccactat
     4081 ttcaaaaata tggccatcct tatcttgtgt gtaacttatt tctattctct ttgcgcctac
     4141 ttgttcagcg ccagcatttg agagcagttc acgaatcaaa tctagagcat gaactctttc
     4201 ttttatcagg tgtttgaaaa tagcaacttc acttactgcc ggtttcacct cacgtgattg
     4261 acttttcacc gcatcgccta tggtattaaa agacatgatt cttccctttc atattcatgt
     4321 aatccctgca caac
//
LOCUS       DAONQZ010000123         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_20768_length_4324_cov_12.284376, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000123 DAONQZ010000000
VERSION     DAONQZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20768_length_4324_cov_12.284376"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLR13_11570"
     CDS             <1..204
                     /locus_tag="PLR13_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20022.1"
                     /translation="GLSIEMEQSIRCLDTDDTMNAMQAYIRYLDQHINAMDPQKMTTE
                     EINQVVQDTVKHMEEGKLYKFKH"
     gene            219..1130
                     /locus_tag="PLR13_11575"
     CDS             219..1130
                     /locus_tag="PLR13_11575"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC20023.1"
                     /translation="MKKTTMGANSKINSSANAILVTGASSGIGWATSLELAQKGWRVF
                     AAVRKEADAKKLRDASSGKITTVIMDIVDYESVKRGASEIEKALGGAGLDALFNNAGI
                     SVQGPLEILPIELFEQQIRVNVFGHIFVTQTFLPLLRKAQGRIVFTSSESGRVTLPLM
                     APYSSSKFALEAVASALRIELRPWKIRVSSVELQTIKTPMWGKIDATTEKLFASVPKQ
                     ARDLYKDELRTLSVFPKWQAEMGISMKKAVRVIIRALSVKRPKARYLVGWEARFLVYC
                     HNVTPIWLMDWFSSKIMILLGKFIKPK"
     gene            1256..2665
                     /locus_tag="PLR13_11580"
     CDS             1256..2665
                     /locus_tag="PLR13_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQC20024.1"
                     /translation="MRDVYIIGVDTIKFGKHMDKSIKDLAITTATGCLKDAGLDKKDI
                     QALWFANAGWGEGGQMTIRGQVALKGMGIEGIPITNVENACAGGSTALHHAWYGVAGG
                     LYDITMAVGAEKLYFSNKNLVFFGFLGGIDIENVLNIAKTLSVFTLTPEEKVKVEEFK
                     QRYQAVKKAANNGGVKKSKAKESKWKAYRDKFTAFIVLGEKIGYDTVRQLARMSSGDH
                     SPFMDVYGFAARQHMKKYGSTAEQMAMIASKNHFNSTLNPNAQYRFDVPVEKVLSDRI
                     VSWPLTRSMCAPIGDGAASAILCSEDMVKKLGLTHQAVKIRASILGSAIRTSFDSDIP
                     EIGERLSKQAYEKAGVGPQDINVAEVHDASAFGEVVQAENLGFCPKGEGGIFAEKGET
                     SLKGRIPINPSGGLISRGHPIGASGLAQVHELVTQLRGNAGKRQVNNARLALAENGGG
                     ALGTEEAAMCIHILEAPSS"
     gene            2812..>3002
                     /locus_tag="PLR13_11585"
     CDS             2812..>3002
                     /locus_tag="PLR13_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20025.1"
                     /translation="MIKPKEVKSLLDREPRFEDSFCSVQAIPKIPKIQLKETRTLVSH
                     ARRFNDEVVRPLALKTDLKT"
     assembly_gap    3003..3102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3103..4119
                     /locus_tag="PLR13_11590"
     CDS             <3103..4119
                     /locus_tag="PLR13_11590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC20026.1"
                     /translation="EPGAGTDVEEVELVDRGKVTCFAEKVKGGYIVNGSKIFISMGHV
                     STWHALIAYTDLKKPSANTLMLAVRTGMKGFTFGKHEDKMGQRACCASELIFQDCFIP
                     DEYVLGDSRDFPNSQIRETSDLVNQIIHYVVEVTRASVGAFGTGVARGAYEHALKFAG
                     ETEVDGKLLINHEWAQIMLAEMYKNVIAGRLAYNEANYSNSLKGGIFNFLQVKPLYYY
                     LKYVPQIVIDTFIVPFYRFKMTDWAIRQIFVEGQSMEAQKRCSGWSSIAKFFGTDMGL
                     KNSQMALELMGQAGLRHDRGAEKFLRDSKLLQIYEGTNQLNRLNLFNCLIARCVPQAR
                     VFEE"
     gene            4131..>4324
                     /locus_tag="PLR13_11595"
     CDS             4131..>4324
                     /locus_tag="PLR13_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQC20027.1"
                     /translation="MLTTVKCELKSFEDLAHNFAAKELAANREANDRYPFGPLFDSVI
                     DKAHDVGLLGATLPEECGGIG"
BASE COUNT         1132 a         1014 c         1132 g          946 t
ORIGIN      
        1 ggtttgagca tcgaaatgga acagagcatt cgctgtctgg atacggatga caccatgaat
       61 gccatgcagg catacattcg ttacttggat caacatatta atgccatgga tcctcagaag
      121 atgacgactg aagaaattaa tcaagttgta caggataccg tgaagcatat ggaagaaggc
      181 aaactttata aatttaaaca ttgatgagga tacgggccat gaagaaaaca accatgggcg
      241 caaattcaaa aatcaattca tcagcgaatg caatactcgt gactggcgcg tccagcggaa
      301 tcggttgggc aacatcgctg gaactggctc aaaaggggtg gcgtgtattt gccgccgtta
      361 gaaaagaggc cgacgccaaa aaactgcgtg acgcctcctc tggcaaaatc acgacggtga
      421 tcatggacat cgtcgactat gaaagcgtga aacgaggcgc gagcgagatc gaaaaggccc
      481 tcggcggcgc gggcctggac gcactgttca acaacgcggg gatttcggtc caggggccat
      541 tggaaatact gcccattgaa ttatttgaac aacagatacg ggtcaacgtt ttcggtcaca
      601 tcttcgtgac gcagaccttt ctgccgctcc tgcgcaaggc gcaggggaga atcgtgttca
      661 cgagttccga gagcggcagg gtcacattac cgctcatggc accatactcc tcatcaaaat
      721 ttgctcttga ggccgtcgcc agcgctctcc ggatagaact tcgcccgtgg aaaatcagag
      781 tctcgtccgt ggaattgcag accattaaaa cacccatgtg gggaaaaata gatgccacca
      841 ccgaaaaatt atttgcatcc gtaccgaaac aggcccgaga cctctataaa gacgagctaa
      901 ggaccctcag cgtctttcca aaatggcagg cggaaatggg gatatccatg aaaaaggccg
      961 tccgggtgat cattcgcgcc ctcagcgtaa aaaggccaaa ggctcgctac ctcgtgggtt
     1021 gggaggccag attcttagtc tattgccaca atgttacgcc aatctggctg atggactggt
     1081 tctcaagtaa aattatgata ctgctgggta aatttataaa accgaagtga acgaacaatc
     1141 gggagccgat aatctgcggg gatcgttcgt tttaaagaat tgttcatgcg gtaaatatat
     1201 gagggcaata atatatttgt tgtaaagaaa gatattcaaa aaatggagaa ataatatgcg
     1261 agacgtctat atcatcgggg ttgatacaat caaattcggc aaacacatgg ataagagcat
     1321 caaggatctg gcgattacga ctgccaccgg atgtctcaaa gatgccggtc tggataaaaa
     1381 agatattcag gctctctggt ttgccaatgc cggctggggt gaaggagggc agatgactat
     1441 tcgcggccag gtcgccctca agggcatggg aatcgaaggc attcccatca ccaatgtgga
     1501 aaacgcgtgc gccggtggat ccacggcgct gcatcatgcc tggtacggag ttgccggcgg
     1561 tttgtatgat atcaccatgg ccgtcggcgc ggaaaaactt tatttttcca ataagaatct
     1621 cgtcttcttc ggatttttgg gaggcatcga tatcgagaac gttctgaata tcgctaaaac
     1681 cctttctgtt tttactctca cgccggaaga gaaggtaaaa gtggaagaat ttaagcagcg
     1741 ctaccaggcg gtgaaaaagg cggcaaacaa tggcggcgtg aaaaaatcca aagcaaaaga
     1801 atcaaaatgg aaggcctatc gagacaagtt taccgccttt attgttctgg gtgaaaaaat
     1861 cggttacgat accgtgcggc agctggccag gatgtcatca ggagatcact cgccgttcat
     1921 ggatgtttac ggtttcgccg cccgccagca catgaaaaaa tatggttcaa cggcggaaca
     1981 gatggccatg attgcctcca agaatcattt caattccaca ctcaatccca acgctcaata
     2041 tcgctttgac gtacccgtgg aaaaagtttt atccgaccgc atcgtctcct ggccgctcac
     2101 gcgctccatg tgcgcgccca ttggcgatgg cgctgcgtca gctattttat gttctgaaga
     2161 catggtgaaa aaactcggcc tgacccatca agctgttaag atcagggctt ccattctcgg
     2221 ttcggccatc agaacatctt ttgattccga cattccggaa atcggcgaga gactctccaa
     2281 acaggcttat gaaaaagccg gcgtcggtcc gcaggacatc aacgttgctg aagtgcatga
     2341 tgcttcggcc ttcggtgaag tggtgcaggc ggaaaatctg ggcttctgtc caaaaggcga
     2401 aggcggaatt tttgccgaaa aaggcgaaac aagcctgaag ggccgcattc ccatcaatcc
     2461 cagcggaggc ctgatttcac gcggtcatcc catcggcgct tccggtctgg ctcaggtcca
     2521 cgaactggtg acccagttga gaggcaacgc cggaaaacgt caggtgaata acgccagact
     2581 ggccctggcg gaaaacggcg gcggcgccct gggtacggaa gaagcggcca tgtgtattca
     2641 tattcttgaa gcaccctcat cctgaaaaga tttacgtcac ccgtgacgtt ttacagctcg
     2701 aaagaaaaag attcagcgtt atcacggtat ccggctccct ggtttgcaga agggggagca
     2761 gtcttcaaaa gggtaagatt gttgcagtca atccacaaag ggagggggat tatgataaaa
     2821 ccgaaggagg tcaaatcttt attagaccgt gaaccgcgtt tcgaggacag tttctgcagt
     2881 gtgcaggcca ttccgaaaat tccgaaaatc cagctcaagg aaaccaggac gctcgtcagt
     2941 cacgcccgtc gcttcaacga tgaagtcgtc aggccgctgg cgctgaaaac cgatttaaaa
     3001 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaacccgg cgccggtacg
     3121 gacgtggagg aagtggaact ggtcgatcgc ggcaaggtga cctgttttgc cgaaaaggtc
     3181 aagggcggat acatcgtcaa cggcagcaag atttttattt ccatgggaca tgtatccacc
     3241 tggcacgccc tgatcgccta taccgatctg aaaaaacctt ccgccaatac gttaatgctt
     3301 gccgtcagga ccgggatgaa gggattcacc ttcggcaagc atgaagacaa gatggggcag
     3361 cgcgcctgct gcgcgagcga attgattttt caggattgtt tcataccgga tgaatatgtt
     3421 ttgggtgatt cacgcgattt ccccaactcc cagattcgcg agacctccga tctcgtcaat
     3481 cagatcatcc actatgttgt cgaggtgacc cgtgcgtcgg taggcgcctt cggaaccggc
     3541 gtggcgcgcg gagcttatga acatgcgctg aaatttgccg gcgagaccga ggtggacggc
     3601 aaactcctca tcaatcacga gtgggcgcag attatgctgg ccgagatgta taaaaatgtg
     3661 attgccggac ggctggctta taacgaggcc aattattcca acagcctgaa gggcggcatt
     3721 tttaatttcc tgcaggtgaa accgctttac tactatctca agtatgttcc gcagattgtg
     3781 atcgatacat tcatcgttcc cttctaccgg tttaaaatga ccgactgggc gattcgtcag
     3841 attttcgtgg aaggacaatc gatggaggcg cagaaacgct gttccgggtg gtcgtcgatt
     3901 gccaaattct tcggcaccga tatggggttg aagaacagcc agatggcgct cgaattgatg
     3961 gggcaggccg gattgcgtca cgaccgcggc gcggaaaaat tcctgcgcga ttcgaagttg
     4021 ctgcaaattt atgaaggcac caatcagttg aaccgcctca atctttttaa ttgcctgatc
     4081 gcgcgttgcg tgccgcaggc ccgtgtattc gaagaatagg agggatgaga atgctgacaa
     4141 ccgtaaaatg cgaactcaag tcttttgaag atctggcaca caattttgcg gcgaaggaac
     4201 ttgccgccaa ccgggaagca aacgaccgtt atcctttcgg tcccctcttt gattccgtga
     4261 tcgacaaagc gcatgacgtc ggactgctcg gtgcgacgct gcccgaagaa tgcggcggca
     4321 tcgg
//
LOCUS       DAONQZ010000124         4285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_21019_length_4285_cov_10.279905, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000124 DAONQZ010000000
VERSION     DAONQZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21019_length_4285_cov_10.279905"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..766
                     /locus_tag="PLR13_11600"
     CDS             512..766
                     /locus_tag="PLR13_11600"
                     /inference="COORDINATES: protein motif:HMM:NF020542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HQC20028.1"
                     /translation="MLSKDIGVEAFWDEEARVWVATSEDVPGLITEADTMEHLMQKLK
                     ILIPELLQANGLLDESVPVEIPIHLLGKWQEVIKAHSKNG"
     gene            759..953
                     /locus_tag="PLR13_11605"
     CDS             759..953
                     /locus_tag="PLR13_11605"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQC20029.1"
                     /translation="MADYTPIVKKILSENGCRFERQGKGDHEIWYSPLTKLRFPVDGK
                     IRSRHTANGVLKQAGLPKQF"
     gene            1007..1222
                     /locus_tag="PLR13_11610"
     CDS             1007..1222
                     /locus_tag="PLR13_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20030.1"
                     /translation="MSTATVNNLIDEFIQLPLDDKEYATEVIKKQLIEAKREAIAKRA
                     KAAMAGFRKKMTKCGTVKELYKDLESD"
     gene            1215..1484
                     /locus_tag="PLR13_11615"
     CDS             1215..1484
                     /locus_tag="PLR13_11615"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HQC20031.1"
                     /translation="MIKISWDQGFKRVYRKKVKNDDELKSRFWGAVEMFAKDPFQPRL
                     RTHKLTGRLEGLWAFSVSFDCRVIFKFLSKTEILLIDIGGHDEVY"
     gene            1671..2765
                     /locus_tag="PLR13_11620"
     CDS             1671..2765
                     /locus_tag="PLR13_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC20032.1"
                     /translation="MGILGSRQVGKTTLAKAIQAVTGKDSIYLDLELPSDMNKLQDAE
                     LYLRQFGKSLVIIDEIQRMPSLFPLLRALVDINRTAGRFLILGSASPDFIRHSSESLA
                     GRIIYHELSPLNYFETDAAKSQKLLLRGGYPNSYLAKNDEESFVWRENYIKTYLEMDI
                     PQLEIRIPSVQLRRFWTMLAHSHGQLWNASKIAASLGITAPTVRRYLDILESTFIIRQ
                     LQPYYTNTKKRLIKAPKVYIRDSGLNHALLRIKTFDDLTGHPSTGASWEGFVIEQIVA
                     LLPENSPYYFYRTNAGAEIDFLYIDQQNRPVPVEIKYSLSPVLSKGFWNAYEDLQCKR
                     GYVVYPGSETYPIGKNVAALPLAQLPTILT"
     gene            2813..3949
                     /locus_tag="PLR13_11625"
     CDS             2813..3949
                     /locus_tag="PLR13_11625"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HQC20033.1"
                     /translation="MCGTEEVYFQTFQRNVGGGIMVTASHNPMDYNGMKLVREGSRPI
                     SGDTGLSDIRTLAEQNIFPKKPSRGSLVKDSNKESYIQHLLGYINLQTLKPLKIVANA
                     GNGCAGPIIDLLAKHLPFEFIRIYHEPDGTFPNGIPNPLLPENRSATANAVIQHKADM
                     GIAWDGDFDRCFFFDENGRFIEGYYLVGLLAQILLEKNPGAGIIHDPHLTWNTIDLVT
                     QGGGTPIQSKTGHAFIKERMRKENALYGGEMSAHHYFRDFAYCDSGMIPWLLIADHLS
                     KTGLPLSVLVDERMLAYPCSGEINFVVDDAKATLNRIRNNFAPENPDMDETDGLSFSF
                     PTWRFNIRLSNTEPLMRLNVETRANKELLRAKVAEITKLITIKT"
     gene            3961..>4285
                     /locus_tag="PLR13_11630"
     CDS             3961..>4285
                     /locus_tag="PLR13_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQC20034.1"
                     /translation="MTTELSEKCILVTGGTGYIGSHTVVALISAGYDPVIVDNLSNSK
                     ITVLERIEAITGKRPPFYQINLRDRTSLDAVFQKHSISAAIHFAGLKAVGESVAEPLR
                     YYENNI"
BASE COUNT         1207 a         1084 c          969 g         1025 t
ORIGIN      
        1 agacggcaga atacgagaga ctggaagcgg aaatccggca tttacaacaa gaagttgacc
       61 ttttagacaa aagcgttacc gaatagggtg catggccgac gattttcttc tcgtcgccta
      121 tcgcccatta cccattaccc attacctata acccgtttcc tgtaacccag agggcggagc
      181 tgtataaaat tagggacagc gccctattaa tttgcggagg agatggatat cggagggact
      241 gtcgcttgac cttcaggtta tcaggttatc cccgaacagt ccgaaaagcg gcgcggttgc
      301 ggctcttgcg gtgcgttcgg cgatcgcgcc caacagttag ccccatagct catctttcac
      361 tcgtcgctcg ttgctcaaaa atagggcgct gtccctaatt ttcttcccca caacgtgctt
      421 tgactttgtc cagctctttg cctataatag aaccagaact taagggcggc ggtaatgcac
      481 ccatctatct atgaaacaaa atggtgaaag catgttaagt aaagatatcg gtgtggaagc
      541 gttttgggat gaggaagccc gggtttgggt tgccacaagc gaggatgtcc ccgggttgat
      601 tacagaagca gacacgatgg agcacctcat gcaaaaactg aagatcctca ttcccgaatt
      661 gctgcaggca aatggcctgc ttgacgagtc cgttcctgtt gaaattccca tccatcttct
      721 tggaaaatgg caggaagtta tcaaagccca ctccaaaaat ggctgactac accccgatcg
      781 tcaaaaaaat cctttctgaa aatggctgtc gttttgaacg gcagggcaaa ggggatcacg
      841 aaatctggta cagccccctc accaagctcc gtttcccggt agatgggaaa atcaggtcgc
      901 gccatacggc gaatggcgtt ctcaaacaag ccggtctgcc caagcaattt tagaataaat
      961 agggcgctgt ccctaatttc ccgaaggaaa aaaggaggcg aaataaatga gtacagcaac
     1021 agtgaacaac ctgatcgatg aatttatcca gttgcccctt gacgacaagg aatatgccac
     1081 agaagtcatc aagaaacagt tgatagaggc aaaaagagag gccattgcca agagggcaaa
     1141 agcagcgatg gccggtttca ggaagaagat gaccaagtgc ggcactgtaa aagaattata
     1201 caaggatctg gaaagtgatt aagatatctt gggatcaggg ttttaagaga gtttaccgaa
     1261 agaaggtaaa aaacgacgat gaactcaaga gcagattttg gggcgctgta gaaatgtttg
     1321 caaaagatcc ttttcagcca cgccttagaa cccataaatt gacgggcagg cttgaagggc
     1381 tctgggcatt tagcgttagt tttgattgtc gcgtaatatt taagttttta agcaagacgg
     1441 aaatccttct gattgatatt ggtggacacg acgaagttta ttgattccgc gcagccaccc
     1501 atcatccatg catcctcact ctacactggg tttaatgtgt gaaaaaatag ggcgctgtcc
     1561 ctaatttctc atttatcctt gcaaatcgaa agcatgtatt ataatatgca agcatgatta
     1621 ggcgctcaat tcagaataac ttaataaatt cattaagaac ttatcctgtt gtgggaattc
     1681 ttggttcgcg tcaggttggc aaaacgacac tggctaaagc cattcaagcc gtaaccggta
     1741 aagattccat atatcttgac ctggaattac catcggacat gaacaaactt caggatgccg
     1801 aactgtattt gcggcaattc ggaaaatccc ttgtcattat tgatgaaatt caaagaatgc
     1861 cttccttgtt cccactgctt cgcgcgcttg ttgatatcaa tagaaccgcc ggccgatttc
     1921 tgatactggg ctccgcatcg cccgatttta ttcgccattc ctcggagagt cttgccggac
     1981 gaatcatcta ccacgagctt tccccattaa attactttga aacagatgcc gctaaatcgc
     2041 agaaactcct cttgcgtggc ggctatccaa acagttacct ggcaaaaaac gatgaggaaa
     2101 gttttgtctg gagagaaaac tatatcaaaa cgtatctgga aatggacatc ccgcagcttg
     2161 aaatccgcat tccttccgtg caacttcgtc gattttggac aatgctggca cacagccatg
     2221 gacaactttg gaatgccagt aagatcgcgg caagtctcgg gattaccgcg ccgaccgtac
     2281 gccgttatct ggatattctg gagtcaacat ttatcatcag acaactccaa ccctattata
     2341 ccaatacaaa aaaacgtctg atcaaggcgc cgaaagtgta cattcgggat tccggattaa
     2401 accatgccct gttgagaata aaaaccttcg acgatctcac aggtcatcct tcaacagggg
     2461 cctcctggga aggatttgtc attgaacaga tcgttgctct tttgccagaa aacagcccat
     2521 actactttta ccgtacaaac gcgggcgcgg aaatagactt cctgtatatc gatcaacaaa
     2581 accggcctgt gccggtggaa attaaatatt ctttgagtcc ggtcctttca aaaggatttt
     2641 ggaatgccta tgaagatttg caatgcaaac gcggctacgt cgtatatccg ggcagcgaaa
     2701 cataccccat aggtaaaaac gtcgctgcat tgcctcttgc acaacttcca acaattttaa
     2761 cctaaaacat agaaaactgt tttctaaaaa tagggcgctg tccctaattt ccctgtgcgg
     2821 gacggaagaa gtgtacttcc agaccttcca gcgcaatgtc ggcggcggta tcatggtcac
     2881 ggcaagccat aaccccatgg actacaacgg catgaaactg gttcgggaag gcagtcgacc
     2941 tatcagtggt gacacgggtc ttagcgatat ccgcacactt gccgaacaaa acattttccc
     3001 caaaaaaccg tctcgggggt cattggtcaa agacagcaac aaggaatctt acatccaaca
     3061 cctgctgggc tacatcaacc ttcaaaccct gaaacccctc aagattgtgg ccaatgccgg
     3121 caacggctgc gcgggcccta tcatagatct gcttgcgaaa catctcccat tcgagttcat
     3181 ccgaatatac catgaaccag atggaacctt tcccaacggc atacccaacc cacttctgcc
     3241 cgaaaaccgg tccgcaacag ccaatgccgt tatccaacac aaagcggata tgggcatcgc
     3301 ctgggatggt gattttgacc gctgcttctt ttttgatgaa aacggacgct ttatcgaagg
     3361 ttattatctg gttggtctgc tggcccaaat cctcttagaa aaaaaccccg gagccggcat
     3421 catccacgac ccgcacctga cctggaacac catcgacctg gtgacccaag gaggcggcac
     3481 accaatccag agtaaaacgg gacatgcctt cataaaagaa cgcatgcgaa aggaaaacgc
     3541 cctctacgga ggcgagatga gtgcccatca ctacttccgg gattttgcct attgcgattc
     3601 gggtatgatt ccctggttat tgattgccga ccacctgagc aagacaggtc tgcctctttc
     3661 cgtcctggtc gatgaacgga tgctggccta cccctgcagc ggcgagatca actttgttgt
     3721 tgacgatgcc aaggcaacct tgaaccgcat ccgcaataat tttgcaccag aaaatcctga
     3781 tatggacgag acggacggct tgagcttttc ctttccgact tggcgcttca acatcaggct
     3841 ttccaataca gagcccctta tgcgcctcaa tgtagaaaca cgtgctaaca aagaactgct
     3901 gcgcgccaaa gttgccgaaa tcacaaagct tataacgatt aaaacttaaa actgtttctt
     3961 atgaccacag aactttcgga aaaatgtatt cttgttaccg gcggaaccgg ctacatcggc
     4021 agtcacaccg ttgttgccct gatctccgct ggatatgatc ccgttattgt tgacaatttg
     4081 tcaaacagca aaataactgt tctggagcgc atcgaagcca tcaccgggaa gcggccgcct
     4141 ttttaccaaa tcaacctgcg cgaccgtacc agcctggatg ccgtctttca aaagcattcc
     4201 atctccgccg ccatccactt tgccggccta aaagccgtgg gagaatccgt cgccgagccc
     4261 ctgcgctact acgaaaacaa catcg
//
LOCUS       DAONQZ010000125         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_21256_length_4244_cov_11.184292, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000125 DAONQZ010000000
VERSION     DAONQZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21256_length_4244_cov_11.184292"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..487)
                     /locus_tag="PLR13_11635"
     CDS             complement(26..487)
                     /locus_tag="PLR13_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQC20035.1"
                     /translation="MIFTLDLRTLDLSNALLSCYASKRPFKEKKTDTSHLTFYLQQGK
                     GGMMSKVQISKKALVVEGGGMRGVFAAGVLNAFGSAGFDPFDMYLGVSAGACNLASHL
                     AGQNDRNFDIIERYSIDGRFINWGRFLRGGHLMDLDWLWEQTIDNRTFVLL"
     gene            complement(583..1164)
                     /locus_tag="PLR13_11640"
     CDS             complement(583..1164)
                     /locus_tag="PLR13_11640"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 family protein"
                     /protein_id="HQC20036.1"
                     /translation="MRLHNIFTVVGIIAAAAWFTVCDKFFHINTGLLVYYWQPLDIIF
                     KGQSVWTFPIFCCATTGFFIATLPFGKQWKENPGYVSMAVNGAVCTLVYFGSGLTGNT
                     HPMIYFWIVLAFWVARILFLREHQAAAVKACLIMGFVGCFWEGALSKLGYFEYYYTDI
                     FNVPLWLFACYLHAGFLMLDLQSFREFFKYSDR"
     gene            complement(1226..3592)
                     /locus_tag="PLR13_11645"
     CDS             complement(1226..3592)
                     /locus_tag="PLR13_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQC20037.1"
                     /translation="MPAFKISEEESRTARLKKQVQEHERGICTERALLWTEYFKNRKN
                     GKKPVMVQMAEALRHVLLNKSVAIYPDELIVGNYTSKRVGGIIYPETHGVPVMLDLLK
                     YPKRAVNPLCISRQEQLDLAMIMPFWQSRFLVAKAFPNLWERARYSLEQLRAGNYYIY
                     EAGGIAHLAPDHEKLIRLGTEEIKKEIEALNLKNTNPEQESFYNAALLSAEALALFGE
                     RYAACAEALAEKEEDAMRSLELHDIAAVCRHVPRRGARTFHEALQSLFFLHVAIFQES
                     MGETVCPGRIDQILYPYYKKDREAHLITRKRAKELLESFCIKLCETIPVYSQAITNLA
                     GGMPSWQVVTMGGQDQEGNDTTNELSYLLLEIATELRMRQPNFHARIHSGSPPEFRKA
                     IYTALAEGSNTPALFNDDVIIPAMTSVGYDIRDARDYVAIGCVEPTAPGKTLGSTDAA
                     ILNIPLAMEFALNQGKRFGSRRREGVNTLPVAKMKTMDDVTTAFIIQLDYLIGRLISD
                     LKAIERAHALYRPLPLTSLFIEGCLSSGLCATQGGAQYNFSGIQGVGISAAGDSLCAI
                     EKLVFQERKLSLKRLRDLLAASEADPYWFTLMQKTPKFGNNNAEADFWTKFVVDEYAR
                     IIHDAGKNTRNGRYLPGIYSNTAHAHFGKMTGALPCGRPRGESFASGMAPQNGMDKSG
                     PTALIHSMNSIDYRHIANGINFNLKLDANSVRDEKGRIFLGALLDVYFRRGGMQIQLN
                     VLDADVLREAKEHPERYPFLLVRISGYSVYFSDLSPRLQDEIIARTSN"
     gene            3860..4204
                     /locus_tag="PLR13_11650"
     CDS             3860..4204
                     /locus_tag="PLR13_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQC20038.1"
                     /translation="MDKKLSNKIVRDIKQRILSGTLKTGDRLPAERELAENYNVSRIT
                     VRNAISRLVYLGFLQTIPQSGTFIADFKKEASVELLVDIVSSDYEVDKAILIELLEIR
                     RVFETYSAGRAA"
BASE COUNT         1098 a         1066 c         1032 g         1048 t
ORIGIN      
        1 tgaagatcaa ctgcgaagga atgatttata gtaggacgaa cgtcctattg tcaatggttt
       61 gctcccacag ccagtccaga tccatcaggt ggccgccgcg aagaaatctt ccccaattga
      121 tgaaccggcc gtctatggaa tagcgttcga tgatgtcgaa gtttctgtcg ttctgaccgg
      181 ccagatgcga cgccagattg caggcgccgg cggaaacccc gagatacata tcgaacggat
      241 cgaatcccgc gctgccgaag gcgttgagaa cgcccgccgc gaacacgccc cgcatcccgc
      301 cgccttccac caccagcgct tttttgctga tttggacttt cgacatcata ccgcccttgc
      361 cctgttgtaa ataaaatgtc agatgagacg tatccgtttt cttttccttg aacggacgtt
      421 ttgatgcata acacgaaaga agcgcgtttg acaaatcaag cgttcgcaag tcaagcgtga
      481 aaatcattac agataaggct tgatattttt acccgtcacg ttcgtgatga cacgcaacgg
      541 cgaaatgaaa ttatcctgtt tcaagtgttc cgcaccggat ttttatctat ccgaatattt
      601 gaagaattcc cggaagctct gcagatccag catcagaaat ccggcatgca gatagcaggc
      661 gaaaagccac agaggaacat tgaatatatc ggtatagtaa tactcaaaat atccgagttt
      721 ggaaagggcc ccttcccaga agcaccctac gaaccccatg atcagacagg ctttgacggc
      781 cgcggcctga tgttcgcgca ggaatagaat acgggcaacc cagaaggcca gaacaatcca
      841 gaagtagatc atgggatgtg tatttcccgt taatcccgag ccgaaataca ccagcgtaca
      901 cacagcgcca tttacggcca tggaaacata acccggattt tccttccatt gctttccaaa
      961 cggcaacgtt gcaataaaaa atcctgtcgt tgcacagcaa aagatgggaa atgtccaaac
     1021 ggactggcct ttgaaaataa tgtccagcgg ctgccagtag tagaccagaa gacctgtgtt
     1081 aatgtgaaag aatttgtcac agacggtgaa ccatgccgcc gcggcgatga tgccgacgac
     1141 ggtaaagata ttgtgcagcc tcatagaatc ctcctgcgca gataatgtta atagccgaaa
     1201 acaaatattc tgatgatcag catgcttagt tgctcgtgcg cgcaatgatt tcgtcctgca
     1261 gccgggggga caggtcgctg aagtaaaccg aataaccgga aatccgcaca agcaggaaag
     1321 gatatcgttc gggatgttcc ttggcttcgc ggagaacgtc ggcgtccaga acattcagtt
     1381 gaatctgcat gccgccgcga cggaaataga cgtcgagcag cgcaccgaga aaaatgcggc
     1441 ctttttcatc acgcacggaa tttgcgtcga gcttgagatt aaaattgatg ccgttggcaa
     1501 tgtgtctgta atcaatgctg ttcatggaat gaatcagcgc cgtcggtccg cttttgtcca
     1561 tgccgttttg cggcgccatg cccgaagcga aagattcacc gcgcggccgt ccgcacggca
     1621 gcgctcccgt cattttaccg aaatgcgcat gcgcggtgtt ggagtaaata ccgggcagat
     1681 accggccgtt acgcgtattt ttcccggcat catgaatgat gcgagcgtac tcgtcgacaa
     1741 cgaattttgt ccagaaatcg gcctcggcgt tgttattccc gaatttcggg gttttctgca
     1801 tcaacgtaaa ccagtacgga tcggcttcag acgccgccag aagatcgcgc aggcgtttca
     1861 gcgatagttt gcgttcctga aagacaagtt tctcgatcgc gcacagcgaa tcgcccgcag
     1921 cggagatgcc cacgccctgt atgccggaaa aattatactg cgccccgccc tgcgttgcgc
     1981 acaagcccga cgaaaggcag ccttcaataa aaagactggt cagcggaagg gggcgataaa
     2041 gagcgtgagc gcgttcgatg gctttcaaat cagagatcag acgtccaatc aaatagtcga
     2101 gctgaataat gaaagcggtg gtaacatcat ccatcgtttt cattttcgca acaggcaggg
     2161 tattgacacc ttcacgcctc cgcgatccga agcgtttgcc ctgattcagg gcgaattcca
     2221 tggcaagcgg aatgttcaaa atcgccgcat cggtcgaacc taatgttttt cccggcgcgg
     2281 tgggctcgac gcagccgatg gccacataat cacgcgcgtc gcggatatca taaccaacgg
     2341 aggtcatagc cggtatgatg acatcatcat tgaagagagc gggcgtgttg ctgccttcag
     2401 ccagcgccgt gtatatcgcc tttctaaatt cgggaggcga tccgctgtgg atgcgcgcgt
     2461 gaaaattcgg ctgtctcatc cgtaattccg tggcaatttc cagcagcaga taactcaatt
     2521 catttgtggt atcgtttcct tcctgatcct ggcctcccat ggtcaccacc tgccagctcg
     2581 gcatgccgcc ggcaaggttg gtaatcgcct gagaataaac gggaatggtt tcgcacaact
     2641 taatgcaaaa agattcgagc agctcttttg cgcgcttgcg ggtgatcaga tgggcctccc
     2701 ggtctttctt ataatacgga taaagaatct gatcgatgcg gccgggacaa acggtttccc
     2761 ccatgctttc ctgaaaaata gccacatgca gaaagaaaag actctgaagc gcttcatgga
     2821 acgtccgcgc cccccttcgc ggaacgtgtc ggcacaccgc ggcgatatca tgaagttcca
     2881 ggcttctcat cgcatcctct tctttttcgg caagagcctc agcgcaggcg gcatagcgct
     2941 caccgaaaag cgccagcgct tccgccgata ggagtgcggc gttataaaac gattcctgtt
     3001 ccggatttgt attttttaga tttaacgctt ctatttcctt tttgatttcc tccgttccca
     3061 ggcggatcag cttttcatga tcgggagcca gatgggcgat gccgcccgcc tcgtagatat
     3121 aataattacc tgcccgcagc tgttccaggg aataacgtgc tctctcccat aaattgggaa
     3181 acgctttggc caccagaaaa cggctctgcc agaaaggcat gatcatggcc aggtccagtt
     3241 gttcttgccg ggaaatacag aggggattca cggcgcgttt cggatatttc aacaggtcca
     3301 gcatgaccgg cacgccatgt gtttcgggat aaatgatacc gcccacgcgc ttcgatgtgt
     3361 aattccccac aatcaattca tcagggtaaa tggcgacgct tttattcaac agaacatgac
     3421 gcaaggcttc ggccatttgc accatgaccg gtttcttccc gttttttcta ttcttgaaat
     3481 attccgtcca caaaagggcg cgctcggtac atatacctct ttcatgctcc tgaacctgct
     3541 ttttaagtct tgcagtacgg ctttcttctt ccgatatttt aaatgccggc atgtgcatac
     3601 gtacacctta aaaaaattaa ttggtaatac caatatgaat aaattttact tattgtcaac
     3661 aaatttcttg ataaatattc atattactgt atattggtat taccaataca agagcaagtt
     3721 gtcaatttaa cgattgtttt tgaatttgag aataataaaa aaatttttgt ctcagactga
     3781 tgaatgacgt ctttagaatc tccggaatgg ttgtatattt tgatttatat cttcgagtca
     3841 gatgtgttaa agaatgataa tggataaaaa actttccaac aaaatcgtgc gggatatcaa
     3901 acagagaatc ttatcgggaa ctctcaagac aggcgatcgt ttaccggcgg aaagggaact
     3961 ggctgaaaat tacaacgtaa gtagaataac cgtccgtaac gcaatttcca gactggttta
     4021 tctgggtttt ctgcagacca taccgcagag cggcacgttc attgccgatt tcaaaaagga
     4081 agcgtctgtg gagcttctgg ttgacatcgt ctccagtgat tatgaagtcg acaaagccat
     4141 tctcatcgag cttctggaaa ttcgccgtgt tttcgaaacc tattccgcgg gccgtgccgc
     4201 ctgaaggtag gacgcgccca gcgtcgtgag cagggtgtag gtct
//
LOCUS       DAONQZ010000126         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_21846_length_4155_cov_13.352683, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000126 DAONQZ010000000
VERSION     DAONQZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21846_length_4155_cov_13.352683"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1543)
                     /locus_tag="PLR13_11655"
     CDS             complement(374..1543)
                     /locus_tag="PLR13_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQC20039.1"
                     /translation="MKKNIIYFDNAATSWPKPPQTLTAMQDYLQNVGGSPGRSGHRLS
                     LEAARIVFETREMLSQLFNVPDPLRIVLTRNATESLNIAIFGLLNPGDHVITSDMEHN
                     SVMRPLRAAEARGVDITVVPCGSNGLIDPGDVVKAIRTNTKAIFITNASNVTGTIMPI
                     ADIGKIAREHDLVLCVDAAQTAGCYPIDVEAMHIDLLAFTGHKSLFGPSGTGGLYIRD
                     GLENKIEPICFGGTGSRSELEVQPNFMPDRYESGTPNTIGIAGLTAGIEFIRSKGLKN
                     ITAHEKQLTGLFLQGIEAIKGVTVYGIKDEYRQIPVVSFNIQGMDPAKVSLELDEQFE
                     IMSRPGLHCAPAAHKTLGTYPSGTVRFSFSCFNTEEQIIKAIEAIRQIGKGRKRK"
     gene            complement(1571..1807)
                     /locus_tag="PLR13_11660"
     CDS             complement(1571..1807)
                     /locus_tag="PLR13_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HQC20040.1"
                     /translation="MSGSPSYCVMLFKSVSYVLKAEKILKKENLPHKLIPVPKKISSD
                     CGVCLRFEPNMREKIEAALFKKVEIEEICKLCEQ"
     gene            complement(1804..2418)
                     /gene="yedF"
                     /locus_tag="PLR13_11665"
     CDS             complement(1804..2418)
                     /gene="yedF"
                     /locus_tag="PLR13_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HQC20041.1"
                     /translation="MSEIRIVNATGLVCPQPVVLAKKAIEKNEQVKVIVDNETALENV
                     RRLGTKMGCTVETQAKAGGLFEILLTRQGGTPVPSENVTVACKTAPAAAGPFVIVCAE
                     DKMGRGNDELGSVLIRAFMHTIAGQAEKPDVMIFYNTGVKLALQGSETLDDIKELSAA
                     GVEILVCGTCLNYFEVKDKLGVGVVSNMYDIAQTMSRASRLLTP"
     gene            complement(2599..3762)
                     /locus_tag="PLR13_11670"
     CDS             complement(2599..3762)
                     /locus_tag="PLR13_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HQC20042.1"
                     /translation="MKADVDPLVNDGFAGEPAKRCLSYIQSQREKGKNVVGIYCGYAP
                     MEVIRAAGMVPAVLCAFADKAIAAGEEVLPTNLCPLIKSSYGFIRTDTCPFYALSQAV
                     IAETTCDGKKKMFELIADIKPTHVMDLPQLPDQKEAQDNWTVMIRKLAGFLEQTFECK
                     VSDAAVEEEIKNTNLKNKLLNQIFDFAALTPSPITWQEIYDLTYLGQVATTEDMLPML
                     KDCLVKLEKRVADGVYHGEKHSPRVLVTGCPVGGDATKVFKIIEDAGGVIVYLDACTG
                     MKAYAGQFEENAQNPYRSVSRRYLNVPCSCMTPNTRRMTELDLIIDKFQPDVVVDVVL
                     HACHSYNVESSRVKAHVEEKHKKPFLKIETDYSQSDVGQIRTRVEALLEMVRK"
     gene            complement(3765..3932)
                     /locus_tag="PLR13_11675"
     CDS             complement(3765..3932)
                     /locus_tag="PLR13_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20043.1"
                     /translation="MIGLRGFNHISIPAEKFGLDVGFIKSPVAMNLVVDDSFLKKAHA
                     KYKKENKSKGK"
BASE COUNT         1013 a         1029 c          932 g         1181 t
ORIGIN      
        1 catcgccttt tttcactgat ttaactttcg ccataatatt gtttcttgcg ccgtgtttca
       61 taatatcctc cttttaatgt catatacttg cttctgaaca gtaagtattt taacaccaat
      121 ggcggaacta taataatgaa ttttaagaat aagaaatata tccgaaaggc ataataaata
      181 taaacctgga caggattcaa ttctttagtt ttgatggttg gcgatgactt tgttgattct
      241 ttaaattatc tggattccgg tttgcaggtc gaaataacgg caattgtggt agagcaaaaa
      301 atcggaaaag cgcgtaatca gaacaaaaag ttttaaacag gaaagattta cggcattaac
      361 tatgtgcctg tttttatttg cgttttcttc ccttgccgat ttgtcttatt gcttcaatgg
      421 ctttgattat ctgctcctct gtattgaaac aactgaaact gaagcgcact gtaccagagg
      481 gataggttcc taaggtttta tgagctgccg gcgcacagtg caagccaggg cgagacataa
      541 tttcaaactg ttcatctagc tccaacgaca cttttgccgg atccatgccc tgaatgttga
      601 aggaaacgac aggtatttgc ctgtattcgt ccttaatgcc ataaaccgtg acacctttaa
      661 tagcttcgat gccctgcaaa aaaagtcctg tcagttgttt ttcgtgagcg gtaatgttct
      721 ttaatccttt tgatctgata aattcaatgc cggcagttaa tccggcaatg ccgatggtat
      781 ttggcgtccc cgattcatag cggtcgggca tgaaatttgg ctgaacttcc agctctgagc
      841 ggctgcctgt tccgccaaaa caaataggct caattttatt ttccagaccg tcgcggatat
      901 aaaggccgcc ggttcccgaa gggccgaaaa gggacttgtg gccggtaaat gctaaaaggt
      961 caatatgcat tgcctcaaca tcaatcggat aacatcccgc cgtttgcgcc gcatccacgc
     1021 ataataccaa gtcatgttca cgggcaatct tgccgatatc ggcaattggc atgattgtgc
     1081 ctgtaacgtt ggaagcattg gtgataaata tcgctttggt gtttgttctg atggctttaa
     1141 caacatcacc gggatcaatc aatccgttac ttccgcaggg aactacagtt atatcgacac
     1201 cccttgcttc cgctgctcgt agtgggcgca tcaccgaatt atgctccata tcggacgtga
     1261 tgacatggtc gccgggattt aagagaccga aaatagcaat atttaaactt tcggtcgcgt
     1321 tcctggttaa aacgatccgc agcggatccg ggacattaaa caattgcgac agcatttctc
     1381 tggtctcaaa aactatacgc gccgcttcca atgacaggcg atgtcctgaa cggccgggac
     1441 tgccaccgac attctgcaga taatcttgca tggcggtcag tgtttgtggc ggcttcggcc
     1501 atgacgtggc ggcattatca aaataaataa tattcttctt cattattata atgatcacaa
     1561 aaccgggttt ttattgttca cacaacttgc aaatttcttc tatttccact ttcttaaata
     1621 gagcggcttc gattttttcc cgcatattcg gttcaaagcg caggcatact ccgcaatccg
     1681 agctgatttt cttgggcacg ggtatcagct tgtgcggcag attttctttt ttgagaatct
     1741 tttccgcttt caggacatag cttaccgatt tgaaaagcat gacgcaataa gatggcgatc
     1801 cgctcatggg gtcagtaaac ggcttgcacg gctcatggtc tgggcgatat cgtacatatt
     1861 tgaaacgacg cccacgccca gtttgtcttt gacttcaaaa tagttcagac aggtaccgca
     1921 gacaagaatc tccacgccgg ccgcggacag ctctttgata tcatcgagtg tttccgaacc
     1981 ttgaagcgca agcttcacac cggtgttgta gaaaatcatg acatccggtt tttccgcctg
     2041 acccgctatc gtgtgcataa acgcgcggat caaaacggat ccgagctcgt cattgccccg
     2101 gcccatttta tcttcggcgc agacgatcac aaaaggcccg gcggctgcag gagcggtctt
     2161 acacgcaacg gttacgtttt ccgaaggcac gggtgtgccc ccctggcgcg tcagcagaat
     2221 ctcaaaaagg ccgccggctt tggcttgagt ttccaccgtg catcccattt tcgttcccag
     2281 acgcctgacg ttttccagcg ccgtttcgtt atctacgata acttttactt gctcgttttt
     2341 ttcaatagct tttttagcca gcaccaccgg ctgcggacag acaagtcccg tggcgttgac
     2401 gattctaatt tcgctcatat tgcactcctt gtatcttgtc gttcgtgatt tgtatcttgt
     2461 atctcgtgaa acgaatctcg taataatctt ccccgtcact cgggggctgt gccgtaattc
     2521 ctttttcgtc attccggaca agcgaagcgc gatccggaat ccagcaagaa atctgtaact
     2581 tgtcgccaaa gtttgcgcct atttccgcac catttccaac aacgcttcca cgcgtgtgcg
     2641 aatctgcccc acgtcacttt gcgaataatc cgtttcgatt ttcaggaaag gctttttgtg
     2701 cttttcttca acatgcgcct tcactctgga ggattcgacg ttataggaat gacaggcatg
     2761 aagcactacg tcaaccacaa catccggttg aaacttgtcg ataattaggt caagctctgt
     2821 cattcgtctg gtgttcggcg tcatgcagga gcagggcaca ttcagatacc tgcgggaaac
     2881 cgaccggtag ggattttgag cgttttcctc aaactgcccc gcgtaagcct tcatgcccgt
     2941 acaggcgtcc agatacacaa tgacgccgcc cgcatcttcg atgattttaa aaaccttcgt
     3001 ggcatcgccg cccaccgggc agccggtgac cagcacgcgt ggcgaatgct tttcgccatg
     3061 ataaacgccg tcggctacgc gcttttcaag cttgacgaga caatctttca gcatcggcag
     3121 catgtcttca gtggtcgcca cctgccccag gtaagtgagg tcgtagattt cctgccaggt
     3181 gatgggcgaa ggcgtaagcg ccgcgaaatc gaatatctga ttgaggagct tatttttgag
     3241 attggtgttc ttgatttcct cctcgacagc cgcgtccgac actttgcact cgaaagtctg
     3301 ctccagaaat ccggcaagct tccggatcat gaccgtccag ttgtcctggg cttccttctg
     3361 gtccggcagt tgcggcaggt ccatcacatg tgtcggcttg atatcggcaa tcaattcgaa
     3421 catttttttc ttgccgtcac aggtcgtttc ggcaatcacc gcctgcgaaa gggcataaaa
     3481 ggggcaggta tccgtccgga taaaaccata actggacttg atgagaggac agaggtttgt
     3541 gggaagaacc tcttcgcccg cggcgatggc tttgtccgca aatgcgcaca aaaccgccgg
     3601 caccatgccc gccgcgcgga tgacttccat cggggcgtac ccgcagtaaa tccccacaac
     3661 atttttcccc ttttcccgct gggactgaat gtaactcaag caccgcttgg ccggttcacc
     3721 ggcaaagccg tcgttcacta aagggtcaac atctgctttc atttttattt ccccttgctt
     3781 ttattttctt ttttatattt agcgtgcgct tttttcagga aagaatcatc aaccacaaga
     3841 ttcatggcaa ccggcgattt gatgaatccc acatccaggc cgaatttttc cgcgggtatc
     3901 gatatatgat tgaaccctct aagtccaatc aataatagtt ataaggaata aagcagtatt
     3961 tcttttattt atttgtgcat cagaataact aagatgatta attttgatcc gatgatgtag
     4021 gcttgtaaca tttgatgaaa tcgacgaaag ttttatgaaa cggcctgaag tcgaagttgt
     4081 gccgataaat taaataaaaa tttctttcca tccagcagct ctgaatggga atttccatca
     4141 gtatcccctg cgcga
//
LOCUS       DAONQZ010000127         4138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_21959_length_4138_cov_13.852070, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000127 DAONQZ010000000
VERSION     DAONQZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21959_length_4138_cov_13.852070"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..79)
                     /locus_tag="PLR13_11680"
     tRNA            complement(4..79)
                     /locus_tag="PLR13_11680"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Glu,seq:ttc)
     gene            complement(311..1012)
                     /locus_tag="PLR13_11685"
     CDS             complement(311..1012)
                     /locus_tag="PLR13_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HQC20044.1"
                     /translation="MSKRNVCITVVLMLLMTAVTCFSADKYQWKLVDTEDNCQIYTSA
                     VPGKDYIAAKATCLIPARIEVIGTILRDIPNYPEWMHDCKATKMLKTVDDEKDVFIFW
                     FRQHIDLLTDRDMILKSKTVIDMQNGTNLVYADSTNELPYDSGKGYVRMPSFYSLFTL
                     QWVDRERTRVTFMIDPNLGSGVPSGIANSKIKSTPFKTLQNMMKMVKKSKYIEAAKTS
                     KYNKMAEDFVKAGHK"
     gene            1230..1595
                     /locus_tag="PLR13_11690"
     CDS             1230..1595
                     /locus_tag="PLR13_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20045.1"
                     /translation="MSEEIHKKSHKEPDEKHLDKMTVKELREIASEIPHEKAVHEMKK
                     EELIAFIKETRGIKDDEPVKKKHIGKVKMTKPELKAKIRECKASRLKALESKETERAV
                     WFRHQISQFKKKLRRIAGA"
     gene            complement(1794..2837)
                     /locus_tag="PLR13_11695"
     CDS             complement(1794..2837)
                     /locus_tag="PLR13_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQC20046.1"
                     /translation="MTNVNDFIGKYRNIITIALSLIGIVLMAYYDYCDTECSYLRGDL
                     LGIDLKWVGIAYMAIIIIFAAFKQTPFVRALLAAGLGVEVHLYAFQIQNNVYCPFCLA
                     FSVMLILSFIINYEVPSAWREKRGRMWLYFLGEVDFPMFKIHKLPLLIFSLLGYLTVF
                     LTFNGSVTPAYGQTPSGAIPSLGKGPYEIIIFTDYFCPPCYRIDTKAEPLLKELLATQ
                     RVKLTFVDVPFNRSTPVYAKYYLYAVQAHSDATSVFRVRKILFDAAQSKKIQKEADLV
                     AYLKNQKVAWKTMDEKSVFPSLTAVIQKNDIRATPSCVIKYSNKDSQKLIGDVEIWKG
                     LTELKARLSAGNK"
     gene            complement(2965..4137)
                     /locus_tag="PLR13_11700"
     CDS             complement(2965..4137)
                     /locus_tag="PLR13_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQC20047.1"
                     /translation="MKEWQKLAWIVGIFLGAYYIPWDSILIRQAGLEAFMMLQDYARQ
                     HVLTCLIPAFFIAGAISVFVSQAAVLKYFGAKANKILSYSVASVSGSVLAVCSCTVLP
                     LFAGIYTRGAGIGPATAFLYSGPAINVLAIILTAKVLGWQLGLARAIGAIFFAIITGL
                     LMAFIFRKDDAEHETGELFLPEEEGNGRALWQDALYMLTMVLILVFASFARPTENDGI
                     VWQMIFSVKWYLVIALVLVLAVMLKKWFVKEELKGWVDSTWGFAKQILPLLLGGVLVA
                     GFLLGRPGHPALIPEQYIQGLVGGNSLWANFFASIVGAFMYFATLTEVPILQGLIGSG
                     MGQGPALALLLAGPALSLPSMIAIAGIMGIKKTSIYCIIVVILSTIAGMTYGWMVA"
BASE COUNT         1195 a          931 c          938 g         1074 t
ORIGIN      
        1 cattggcgtc cccacgcgga ttcgaaccgc ggttacaggc gtgaaaggcc cgtgtcctag
       61 gcctctagac gatggggacg tccttaaagc cgtttcaagc aagttgtgtc ttatatattg
      121 ataaaatcag aatgtcaagc gttgactcaa aaaaataacg gaaagaatcc gccatttttt
      181 tgttgtttta aatacccggt tattatcaga ttttcccgct gtctgtcgct atatatcaat
      241 ccataaaaat aggaaaggcg gatcatcttt gaaccgcctt tcctaagtga tttatgctga
      301 acggcaatgt ttatttatgc ccggctttta cgaaatcctc ggccatcttg ttgtacttgg
      361 aggttttggc cgcttctata tattttgatt ttttcaccat cttcatcatg ttctgtaaag
      421 ttttaaaggg tgtcgatttg attttgctgt tggcaattcc ggatgggacg ccggatccga
      481 gattcggatc gatcataaac gtgaccctgg tgcgttcccg gtctacccac tggagagtga
      541 acagtgagta aaatgaaggc atccggacat atcccttgcc ggaatcatag ggcagttcat
      601 ttgtggagtc ggcataaacc aggttggttc cgttttgcat atcaatcacg gttttacttt
      661 tcagaatcat atcccggtct gtcaacagat caatatgctg acggaaccag aagataaaaa
      721 catccttttc atcatcgacc gttttcaaca tttttgttgc tttgcagtcg tgcatccatt
      781 cgggatagtt ggggatgtct ctcaaaattg taccgatcac ttctatgcgt gcaggaataa
      841 ggcatgtggc tttggcggca atgtaatctt tgccgggaac tgcgctggta tagatttggc
      901 aattgtcttc tgtgtcaacg agtttccatt gatatttatc agcggaaaaa caggttacgg
      961 cagtcatcaa aagcatcagc acgacggtaa tacagacatt tcttttagac ataaaaatcc
     1021 tcctgtagtg tatttttaat acccttcttc tgctcttctt atgcggtgat gttgatgtgg
     1081 tgtatcgttt ccttacacgg tgcggttgag attgtcaaga aatatggaag cggattgaac
     1141 cacgtgaaaa cttatgatat aaggtaggca cactcattcg tcgcaaattt gattcctgaa
     1201 tataaaaaat gtaaggaggt ctatgcgcga tgtcagaaga aatccacaaa aaaagtcata
     1261 aagaaccgga tgagaagcac ctggacaaaa tgacggtcaa agaacttcgg gaaattgcgt
     1321 ccgaaattcc tcatgagaag gctgttcacg agatgaaaaa agaggaactc atcgccttta
     1381 taaaggagac ccgggggatt aaagatgacg agccggtcaa gaagaaacat atcggcaaag
     1441 tcaaaatgac caaacccgaa ttaaaagcta aaatacgaga gtgcaaggca tcaagattaa
     1501 aagcgctgga atcgaaggag actgaaagag ccgtctggtt cagacatcag atcagtcaat
     1561 ttaagaagaa attgcggcgt atcgcagggg cttgatttgc actgctggaa ggtgtcgccg
     1621 tcacggtgtg attccccatg caaatcgcac atatgatctt ttgcgccgtg cgggatccta
     1681 caatgcgtga attggattgt gccggaaaag attccggcac aatgtttttt aaatttgggt
     1741 cttggcaaag agtatcgggt gtttatatgg acttcgggac atcgctcagg tcatcacttg
     1801 tttccggctg ataggcgtgc cttcaattcc gtcaatccct tccatatttc tacatcaccg
     1861 ataagctttt ggctatcctt gtttgaatat tttatgacac atgacggtgt cgcccggatg
     1921 tcattttttt gaatcacagc cgtcagcgat gggaaaacgg atttttcatc cattgttttc
     1981 cacgcaactt tttggttttt taaatatgcg acaagatctg cttctttctg aatttttttg
     2041 gattgcgccg catcaaaaag gatcttacgg actcgaaaaa cacttgttgc atcggaatgg
     2101 gcttgcaccg cgtacagata atatttagca taaacaggtg tcgatcggtt aaaggggaca
     2161 tcaacaaatg tcagttttac ccgctgggtg gccaataatt ctttcagcag gggctcggct
     2221 tttgtatcga ttcgataaca gggcggacaa aaatagtcgg taaagataat aatttcatag
     2281 ggccctttgc ccagcgaagg aatggcaccg gacggcgttt gaccataagc cggtgtgacg
     2341 gatccgttga aggtcaaaaa aacggttaaa tatcccagta aactgaaaat cagcaggggc
     2401 aatttatgaa ttttgaacat cggaaaatca acctccccga gaaaatacag ccacatccga
     2461 ccgcgttttt cccgccatgc ggatggaact tcatagttaa tgataaatga caaaattaac
     2521 attaccgaaa atgcaagaca aaacgggcaa tagacattat tttgtatctg aaaagcgtat
     2581 aaatgaacct cgacgccaag accggcagcc agaagagccc ggacaaaagg tgtctgttta
     2641 aaagcggcga agattatgat gatggccata taagcgatac cgacccattt gagatcgatt
     2701 cccagcaggt cgcctctcag atagctgcac tctgtatcgc aataatcata ataggccatc
     2761 agaacgatgc cgatcagcga caacgcaatg gtaataatat tccgatattt tccaataaaa
     2821 tcatttacat ttgtcatgcc tgatgtcctc aataccttat gttttatttt agctcaagaa
     2881 aaatattttt caagatatgt tgagcatatt ttatcgttaa tcttttgtcc cgacaatttg
     2941 gcgtgttcat cgcctgcgga tttttcaggc aaccatccat ccataggtca tgccggcaat
     3001 ggttgagaga atgaccacaa tgatgcaata gatggacgtt ttttttatcc ccatgatgcc
     3061 ggcgatggct atcatactgg gcaatgaaag cgccggtcct gccagaagaa gagccagcgc
     3121 cggtccctgg cccataccgg aaccgatgag tccctgtaaa ataggaactt cggtcagtgt
     3181 ggcaaagtac ataaaagcgc cgacaatgga agcaaaaaag tttgcccata acgagttgcc
     3241 gccgacaagg ccttgaatat attgctcggg aatcagtgcc ggatgaccgg gacgtcccaa
     3301 aagaaagcct gccaccagta ctccacccag caaaagcggc agtatctgtt tggcaaaccc
     3361 ccaggtggaa tccacccatc ctttgagttc ttccttgaca aaccatttct tgagcatcac
     3421 cgccaaaaca agcactaatg caatcaccag ataccatttg acgctgaaaa tcatttgcca
     3481 gacgatccca tcgttttcgg tcggtcgtgc gaacgaagca aaaacaagaa tcaacaccat
     3541 cgtcagcata taaagcgcat cctgccagag cgcccgtccg tttccctctt cttcaggaag
     3601 gaatagttcg cctgtttcat gttcagcatc atccttgcgg aaaataaacg ccatcaatag
     3661 accggtgata atcgcgaaaa aaatcgctcc gatggcgcgc gccaatccca gttgccagcc
     3721 aagaactttg gcggttagaa taatggccag cacgttgatg gccggcccgg aatacaaaaa
     3781 tgccgtggcg ggaccgatgc ccgcgccgcg ggtgtaaatt cccgcgaaaa gcggcaacac
     3841 ggtgcaggaa caaaccgcca gcaccgaacc ggaaacggaa gcgactgaat aagacaaaat
     3901 tttattcgcc ttcgcgccaa aatactttag cacagccgcc tgcgatacaa aaacgctgat
     3961 ggctccggca ataaaaaaag caggaataag acaggtcagc acatgctggc gcgcgtagtc
     4021 ctgaagcatc atgaatgcct ccaggccggc ttgtcgtata agaatcgaat cccaggggat
     4081 ataatatgcc cccagaaata ttccaacaat ccacgctaat ttctgccatt ctttcata
//
LOCUS       DAONQZ010000128         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_22024_length_4128_cov_10.908421, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000128 DAONQZ010000000
VERSION     DAONQZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22024_length_4128_cov_10.908421"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLR13_11705"
     CDS             complement(<1..878)
                     /locus_tag="PLR13_11705"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQC20048.1"
                     /translation="MAAKIKVKTTVVEMDGDEMTRVMWLMVKDKLLFPYLDMDIDYYD
                     LHVKHRDDTDDKVTVDAAKAIMKYGVGIKCATITPNEDRVKEYRLKKAWKSPNGTIRA
                     MLDGTVFRKPILVNNIKPNVSSWKKPIIIGRHAYGDVYSNTEFDVPSAGKLELVFTPQ
                     DGGKAVSVPVFDFPGKGIAQVNYNLEQSIVSFARACFTYALSEKIDLWFSTKDTISKI
                     YDAGFREIFQQEFEKNWKNKFDQAGIEYFFTLIDDAVARVMKSEGGMLWALKNYDGDV
                     MSDMVASACGSLAMMTS"
     gene            complement(1023..2954)
                     /locus_tag="PLR13_11710"
     CDS             complement(1023..2954)
                     /locus_tag="PLR13_11710"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQC20049.1"
                     /translation="MAQNLVEKILSKHLVSGTLIPGEEIGIRIDQTLTQDATGTMAYL
                     QFESMNMPKVKTELSVSYVDHNTIQIGFENADDQLYLQTVAQKYGMVFSRAGNGICHQ
                     IHLERFGKPGKTLIGSDSHTPTCGALSMIAIGAGGLDVALAMAGKPFYLVCPKVIKIN
                     LTGKLKPWISAKDVILKVLEIFSTKGNVNTVFEYGGKGVESLTIPERATIANMGAECG
                     VTTSVFPSDKMTRLFLRSQHREKDFVELKPDKDAKYVKVVNIDLSKIEPLTAKPHSPD
                     NISTVKKMQKIDVHQVCLGSCTNSSYKDLVTVAKILKGKTVHPNVSFILAPGSRQVLK
                     ELSKNGYLTDLISSGARLMENACGFCIGNSQSPSSAAVSLRTSNRNFQGRSGTMDANV
                     YLVSPETAAAAVITGKFTDPRDLKMRYPKVTLPKKFSIDDTMIIRPDKKKKDVEVIRG
                     PNIGILPRSPELSDTIHGPVTIKVGDKITTDHIIPAGARMKYRSNIPKYSEFVFENVD
                     ATFAKRAMSIRQDGKQNIIVAGHSYGQGSSREHAALCPMYLGVRVVIAKSFERIHTAN
                     LINFGIIPLTFCSESDYDIIEMEDLLEITETKKTIAGDGKFLMRNKTKGTAFYVTCDL
                     SERQKQIILAGGALNINKA"
     gene            complement(3158..>4128)
                     /locus_tag="PLR13_11715"
     CDS             complement(3158..>4128)
                     /locus_tag="PLR13_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQC20050.1"
                     /translation="QMTVFSGLFVLFLLFGLSSNAFAIHYKVKNGDTLYSISKKFNVS
                     VIDIKKANNLKKTTIKANQVLKIATSPKGTSKSTVSKNTISSYYVVKKGDTLSKIAQK
                     NRIPLKKLMALNNIHSKKIRAGQKIVLRASDPPSRVKTVVAEDRDEDIIDDTENAAVN
                     EDPAVEENYDGEEGFFSNIFNFGYQDDFLGKWKSTDEMQRLIKKATGFLGAPYRLGGN
                     SKRGIDCSALVQKVYGSFNVKLPRVARDQSKVGVEVDIDELVEGDLVFFHTNRSFGHV
                     GIYIGNNEFIHASSRNRRVRIDSLDHPFYQERFQRAVRIKELPVGG"
BASE COUNT          980 a          967 c          983 g         1198 t
ORIGIN      
        1 gatgtcatca tggccagcga accgcaggcg gaagcgacca tgtccgacat gacgtcgccg
       61 tcgtagtttt tcagcgccca gagcatgccg ccctcgcttt tcataacccg cgccacggcg
      121 tcgtcgatca gggtaaagaa atattcgata cccgcctggt caaatttatt cttccagttc
      181 ttttcgaatt cctgctggaa aatttcccgg aatccggcat cgtatatttt ggagatggtg
      241 tctttggtgg aaaaccacag atcgatcttt tcgctgaggg cgtaggtgaa acaggcccgg
      301 gcaaaactca ctatggattg ttccagatta tagttcactt gggcaatgcc tttgccgggg
      361 aagtcaaata ccggaacgct gacggccttg ccgccgtcct gcggcgtaaa gaccagttca
      421 agtttgccgg cggacggaac atcaaattct gtattgctgt atacatcgcc gtaggcgtga
      481 cggccgatga tgatgggttt tttccaggag gaaacattcg gtttgatatt gttgaccaga
      541 atgggtttcc ggaaaaccgt gccgtccagc atggcgcgga tggtgccgtt ggggcttttc
      601 catgcttttt tcagacggta ttctttgaca cgatcttcgt tgggggtgat ggttgcgcat
      661 ttgatgccga cgccgtattt cataatcgct ttggctgcat cgacggtgac tttgtcgtct
      721 gtatcgtcgc ggtgttttac atgcagatca taatagtcga tatccatgtc cagatagggg
      781 aaaaggagct tgtcctttac cattagccac atcacgcggg tcatttcatc gccgtccatt
      841 tcgacgacgg tggttttcac tttaattttt gctgccataa aaaaaattac tgctccttca
      901 tgagaaccaa gcttcagaac aaagtgcgct gccgcttgct tattataatg aaacgtgaac
      961 ttcaacagcc ttgcgtattg aagttttggg ttgaatgaac tcccgcagtc tgccgcgggg
     1021 ccttatgcct tattgatatt cagggctcct ccggccagaa tgatctgttt ctgcctttcg
     1081 gacaaatcgc aggtgacata aaaagcggtt ccctttgttt tgtttctcat cagaaatttt
     1141 ccgtccccgg caatggtctt ttttgtttca gtaatttcaa ggagatcttc catctcaatg
     1201 atgtcgtaat cgctctctga acagaatgtc agcggtatga tgccgaagtt gatcagattg
     1261 gccgtgtgaa ttctttcaaa agatttggca atgacgaccc gtacccccag atacatcggg
     1321 cacagcgcgg catgttcgcg ggaagatccc tgaccgtaag aatgaccggc gacaatgata
     1381 ttttgtttcc cgtcctgcct gatggacatg gcccgcttgg cgaatgtcgc atcgacattt
     1441 tcgaaaacaa attccgaata tttcggaata ttcgaacggt atttcatccg cgcaccggcc
     1501 ggaatgatgt ggtctgtcgt gattttgtcg ccgaccttga tggttaccgg tccgtgaatg
     1561 gtatcggaaa gttccgggct gcgcggcaga atgccgatgt tcgggccgcg gatgacctcg
     1621 acatccttct tttttttgtc cggacgaata atcattgtat catcaatgga aaacttcttc
     1681 ggcagcgtga ctttcggata gcgcatcttc agatcgcgcg gatccgtgaa tttcccggtt
     1741 ataaccgccg ccgcggcagt ctccgggctg accagataaa cgttggcatc catcgtgccg
     1801 gagcgtccct gaaaattacg gttggacgtg cgcaacgaca cggcggctga cgaggggctc
     1861 tggctgttgc cgatgcagaa tccgcaggca ttttccataa ggcgtgctcc cgaggagatc
     1921 agatccgtca ggtaaccgtt tttggacagc tccttgagca cctgacgcga accgggagcc
     1981 aaaataaagc tgacattggg atgcacggtt ttgcccttta aaatttttgc cacggtaacc
     2041 agatctttat aagacgagtt ggtgcagctt cccaggcaaa cctgatggac gtcgatcttc
     2101 tgcatttttt taaccgtgct aatattgtcc ggactgtggg gcttggctgt taacggttct
     2161 attttggata aatcgatatt aacgacttta acgtatttag cgtctttgtc gggtttcaac
     2221 tcgacaaagt ctttttctct gtgctgtgat ctcaggaaga ggcgcgtcat tttatcgctg
     2281 ggaaagacgg atgttgttac gccgcattcg gcccccatgt tggcgatggt tgcgcgctcg
     2341 gggatggtca aagactcaac gcccttgccg ccgtactcaa ataccgtgtt aacgttgcct
     2401 tttgtagaaa agatttccag caccttgaga ataacgtcct ttgcggaaat ccagggttta
     2461 agtttgccgg tgaggtttat tttaatcact ttcgggcaga ccagataaaa cggctttccg
     2521 gccatcgcca gcgccacatc cagtcctccc gcgccgatgg cgatcatgga gagtgccccg
     2581 caggtgggcg tatggctgtc cgacccgatc agggtctttc cgggtttgcc gaaccgttcc
     2641 agatggatct gatgacagat gccgttgccc gcacgggaga acaccatacc gtatttctgc
     2701 gctacagtct gcagatacag ttgatcgtcc gcgttttcaa agccgatctg aatggtattg
     2761 tgatcaacgt aactgacgga caactccgtt tttaccttgg gcatattcat ggattcaaac
     2821 tgcagatacg ccatggtgcc tgtggcatcc tgagtcaggg tctgatcaat acgaattccg
     2881 atttcttccc cggggataag agtacccgag accaaatgct tcgacagaat tttttctacc
     2941 aggttttgtg ccatttttta ttccttgaag agaagaatta atgttctaaa gcatttacag
     3001 ccgcgaactt atataataat cttaaccgat aatcaactgt caaaaggtaa gaaattttta
     3061 aaaaataaag ggctttgcaa tcgttttaca aagcccagtt atttatggaa atgaatgatg
     3121 gataaaccca tttaaaaaca aaaggttatc ctgattttca gcctccaacc ggcaactctt
     3181 ttatcctgac ggcgcgttga aatctttcct gataaaaggg atgatccaac gagtcaatcc
     3241 ggacgcgtct atttcttgaa gaggcatgga tgaattcatt attccctatg taaattccga
     3301 catgtccgaa cgaacgattc gtatgaaaga ataccagatc tccttcgacc agttcgtcga
     3361 tatccacctc cacaccgacc ttggattgat cacgggcaac gcgcggcagt ttgacattga
     3421 aactgccata gactttttga acgagtgcgg agcaatctat tcctcttttt gaatttccgc
     3481 ccagtctgta aggcgctccc agaaatccgg ttgccttttt aatcagcctt tgcatttcat
     3541 ccgtgctttt ccattttccc agaaaatcat cctggtatcc gaaattaaag atattggaga
     3601 aaaatccttc ttccccgtca taattttctt ccactgccgg atcttcattg acggcagcat
     3661 tttctgtatc gtcaatgata tcttcatccc gatcttccgc aacgactgtt tttacacggg
     3721 atgggggatc agaggccctt aaaacaattt tctggccggc acgtatcttt ttggaatgaa
     3781 tgttattcaa agccattaat tttttcagcg gaatgcggtt tttttgtgca atcttcgaaa
     3841 gggtgtcccc ttttttgacg acgtaatatg atgatatggt gttttttgaa accgtcgact
     3901 tggatgttcc tttgggggaa gtggcaatct ttaaaacttg attggcttta atggttgttt
     3961 ttttcaggtt gttagctttt ttaatgtcaa tgacgctaac attgaatttt ttggaaatac
     4021 tgtaaagggt gtcaccgttc tttactttat aatgtattgc aaaagcgtta ctgctaagac
     4081 cgaacaacaa aaaaaggaca aagagtcccg agaatacagt catctgcc
//
LOCUS       DAONQZ010000129         3983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_23067_length_3983_cov_13.966141, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000129 DAONQZ010000000
VERSION     DAONQZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23067_length_3983_cov_13.966141"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..677)
                     /locus_tag="PLR13_11720"
     CDS             complement(159..677)
                     /locus_tag="PLR13_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC20051.1"
                     /translation="MNTNEDIERFRKLAETGDADAQYHLGLIYQNEKGVSLDYAQAVK
                     WLVMAAEQGHADAQYNLACIYNNGKGVSLDYPKAIKWFTRAAEQGHAHAQYMLGLMYS
                     SGLQDYVEAARWFLKAAEQGIADAQFKIGVILEEGRGVRKDPASAEVWYGEAAEQGHA
                     DAKKRLDKMQNK"
     gene            917..1342
                     /locus_tag="PLR13_11725"
     CDS             917..1342
                     /locus_tag="PLR13_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQC20052.1"
                     /translation="MNTKIGLSQILLYFLMLMFMMVIGCGDGADDFKLPKYHDADADK
                     CTISGRVTYKGVGRAGITVLLSGYSNATVATDENGEYRFDDVDGDQAYVVSPSSGTLI
                     FTPESIGVEPDGSNVDGVDFDAMDCCGDDTSEEEETEEI"
     gene            complement(1489..>2708)
                     /locus_tag="PLR13_11730"
     CDS             complement(1489..>2708)
                     /locus_tag="PLR13_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQC20053.1"
                     /translation="DILPLSFFENADYPGSPPIECLVLYKKKTSLPAARESFLETIRH
                     YNLFSSRLIMIDENRFALLYCTDGFQYMVLPPLDADAAHISIDDVRHMMRHVKTLPGE
                     PLFAVTIIPVRDGLLGGISCSHAIGDAVSLMLFLYAWGCIHYGNSFSKPSTQRLFSGN
                     PVDFDTFDSVFAPPVSDLHETIRKRAENSQETTRYHRREYFSDKRLAEMKNGATSENE
                     NYRLSNNQIINAVLLKKYHNGIMPGTDQIRLRIPINLRYIHPDVDPLYIGNAVFSAVI
                     GFSRAEINRMSVYRIACRLKEAIAKMRNEHYVRSIACLSKFGIRYRGDIFKTLPPYNP
                     DTDILAANLTHVNDLESLGLGDDVGRILYMGSTMQTSFTMLKEKHNRLFAEIRSRYPL
                     ESPTTTHGTAEVP"
     assembly_gap    2709..2808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2825..>3983)
                     /locus_tag="PLR13_11735"
     CDS             complement(2825..>3983)
                     /locus_tag="PLR13_11735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQC20054.1"
                     /translation="NISDMVEPETYFRFAEVLKEELALDRQPGRDLKRSRTLEYQHIF
                     KDRSKKWMEATLTFLRDKNGEAVGIIGIARDISERKAMEEKLQFEERRFRNFVEHSAD
                     IIVLVNLEGLITYINPAIERVLGYKTEERIGRRGFELVHPDDLEFLIQSFTTLATQPD
                     LPPIHGEMHLRHKNGTYRTLEAVGSNLVKDHVVEAIIINYRDITERKLAEEALQRSEQ
                     RYLELSILDDLTQVFNSRHFHAELGKEIERSTRYDHPLSLLLLDLDHFKTFNDTYGHV
                     EGDNVLSRLGQVVKKCLRDSDSAYRYGGEEFTIILPMTTKEDAVSTAQRIQNEFRKEV
                     FTPLQGKEIFVTMSIGASQYIPKEEMKSFVHRVDQFMYQAKQNGRDRICSD"
BASE COUNT          958 a          925 c          912 g         1088 t
ORIGIN      
        1 aaaacagggg gaaactttac gatgaaaaag tggttgacgc ctgtctgaaa ctctttagag
       61 agaagggcta caaacttata taaaaggaaa aaactgaaat ctcgggtatg atccttctcg
      121 aggtttcatc ctgttttttt tgatgaacat cgccgccgct atttgttttg cattttgtcc
      181 agtcgttttt tggcatccgc gtggccctgc tctgcagctt cgccgtacca cacctcagcc
      241 gaagcaggat cttttctgac gcctctgcct tcttccagaa tcacgcctat cttgaactgc
      301 gcgtcggcaa tgccctgttc cgctgccttt aggaaccatc gcgcggcttc aacataatcc
      361 tgtagcccgc tgctgtacat caatcccagc atatactggg catgggcatg accctgctca
      421 gcagcccggg taaaccattt gatcgccttc gggtaatcca acgagacgcc tttgccgttg
      481 ttgtaaatac aggccagatt atactgggca tcggcatgtc cctgctcggc ggccatcaca
      541 agccatttaa cagcctgagc ataatccaga gatacccctt tttcattctg atatatcaag
      601 cccagatgat actgggcgtc agcatcacct gtctcggcca attttctgaa acgctcaata
      661 tcttcattcg tattcatttt gaaattccac accttttcat ttgaggaaac attatcataa
      721 ttgatagcgg aaatctatac ggatctgctt gatgatgcat gtgaaaatcc ccgggtcttc
      781 catgattgat gaaaacggtc tctgtcgaaa ttatacgcat aaccattcct tgacattcaa
      841 acggtaattc catataaata aacctatcaa taaaagtttt agcaaattcg attagcgtca
      901 tctttggggg atctttatga atacaaaaat tggtctatct cagattttat tgtattttct
      961 tatgctgatg ttcatgatgg tcataggttg cggcgacggg gcggatgatt ttaaattacc
     1021 aaaatatcat gatgccgatg cggacaaatg caccatttca ggcagagtta cctataaagg
     1081 tgtcgggcgt gcgggtatta ccgttctcct gtcggggtac agtaacgcta ctgtagctac
     1141 agatgaaaat ggagaatacc gatttgacga cgtcgatggc gaccaggcct atgttgtcag
     1201 cccgagcagc ggaacgctga tttttacacc cgaaagcata ggcgtggaac cggacggatc
     1261 aaatgttgac ggtgtggatt tcgatgcgat ggattgctgc ggtgacgata catccgagga
     1321 agaggaaacg gaagaaatct gatcaaccac ggcaattggt gggtcatgcc ctcgcttagt
     1381 atcggcggtt tgcaggacat cggaatgtct ttacttaaaa tcataaatat cactgtataa
     1441 gtctcgatga gctgccgtaa aatgaccgat gttgttgcag gcgtgctgtt atggaacttc
     1501 agcagttccg tgagttgttg ttggactttc cagcgggtat ctacttctta tttctgcaaa
     1561 taatctgttg tgtttttcct tgagcatggt aaaacttgtc tgcatcgttg aacccatgta
     1621 cagaattctt ccgacatcgt ctcccagtcc aagcgattcc agatcgttca catgcgtcag
     1681 attggccgca agaatatccg tgtcgggatt atagggcggc agcgttttaa aaatgtcgcc
     1741 ccgatacctt atcccgaatt tcgacagaca ggcgatgctt ctgacataat gttcattcct
     1801 catttttgct atggcctcct taagacggca ggcaatccga tagacggaca tccggttgat
     1861 ctccgctcgg gaaaagccga taacggcgga gaaaaccgcg ttgccgatgt acagcggatc
     1921 aacatcggga tgaatatatc gcagattgat ggggattctc aatctgatct gatccgtgcc
     1981 gggcatgatg ccgttgtgat actttttcaa taaaacggca ttgatgattt gattgttgga
     2041 caggcgataa ttctcatttt cggacgttgc cccgtttttc atttccgcaa gcctcttatc
     2101 tgaaaagtat tcacgtctgt ggtaccgcgt cgtctcttgt gaattttcgg cccgcttgcg
     2161 gattgtttca tgcaagtcgg agaccggcgg ggcgaaaacc gaatcaaagg tgtcgaagtc
     2221 aacagggttc cccgaaaata atcgttgcgt cgacggtttg gaaaaactgt tgccgtagtg
     2281 aatgcacccc caggcataaa gaaaaagcat cagcgaaaca gcatcgccaa tggcatgaga
     2341 acaactgatc ccgcccagca atccgtctct taccgggata atggtgacgg caaacaaagg
     2401 ttctcccggt aatgttttga catgtcgcat catgtgtcgg acatcatcga tcgagatgtg
     2461 agccgcatcc gcatccagcg gcggtaaaac catatattga aaaccgtccg tacagtacag
     2521 aagcgcaaac ctgttctcat caatcatgat caggcggctg gaaaacagat tgtaatgtct
     2581 gatcgtttca agaaaacttt ctcttgctgc cggaagcgat gttttcttct tgtaaagaac
     2641 cagacattcg attgggggac tgcccggata atctgcattt tcgaaaaaag acaagggcag
     2701 tatatcgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggtaacgaat
     2821 gctgtcaatc gctgcagatt cggtccctgc cgttttgttt ggcctgatac atgaactggt
     2881 cgacacgatg aacgaaactt ttcatctctt ctttcggtat atattgcgag gcaccgatac
     2941 tcatcgtcac gaaaatttct tttccctgca ggggggtaaa gacctctttc ctgaattcat
     3001 tttggatcct ctgggccgta ctcacggcat cctcttttgt tgtcatgggc agaataattg
     3061 taaattcctc gccgccgtaa cggtacgccg aatctgaatc gcgcaagcat tttttgacga
     3121 cctgaccgag ccgggataga acattatcgc cttccacgtg gccgtaggtg tcgttaaatg
     3181 tcttgaagtg atcaagatcc agaagcagga gcgacaatgg atgatcgtag cgggttgacc
     3241 gttctatttc ctttcccagt tcggcgtgaa aatgtctgga attaaaaacc tgtgtcaggt
     3301 cgtcaaggat gctcagctcc agatatcgct gttcgcttct ctgcagagct tcctccgcca
     3361 gtttgcgttc ggtgatatcc cggtaattga taatgatcgc ttccacgaca tgatctttga
     3421 cgagattgct tcccacggct tccaatgtgc gataggtgcc gtttttgtga cgcagatgca
     3481 tttcgccgtg aatcggcggc aaatcgggtt gggtggccaa ggtcgtaaac gattggatta
     3541 aaaattccag gtcatccgga tgaacaagtt cgaatcctct gcgtccgatt ctttcttctg
     3601 tcttgtatcc cagtacccgt tcaatggccg ggttgatgta tgtgatcaat ccttccagat
     3661 tgaccagcac aatgatgtcg gcagagtgct ccacaaaatt ccggaatcgc cgttcttcaa
     3721 actgtaattt ttcctccatc gccttgcgtt cggaaatgtc ccgggcgatg ccgatgattc
     3781 caacggcctc gccgttttta tcccttagaa aggtcagggt ggcttccatc catttctttg
     3841 atcgatcctt gaagatatgc tgatattcca gcgttcgcga ccgttttaaa tcccgtcccg
     3901 gctgtctgtc caacgccagc tcttccttca gaacttcggc aaaacggaaa taagtttccg
     3961 gttcgaccat gtcggaaatg ttc
//
LOCUS       DAONQZ010000130         3840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_24249_length_3840_cov_15.893263, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000130 DAONQZ010000000
VERSION     DAONQZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24249_length_3840_cov_15.893263"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..770)
                     /locus_tag="PLR13_11740"
     CDS             complement(93..770)
                     /locus_tag="PLR13_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20055.1"
                     /translation="MKSTQMIVRLAIILLSMSGIFAGAIRAESLNDGLGPQATQALGE
                     KNVLTVVVRFPDATPSLPIEAVRRKVVGGLNAYVKEQSYGLASIKADFMGYVMLPDSI
                     ASYNISPYNFRVDRRRVRKLIEDTMTALESRVDFSAYDHILIVPAVTTMPGKGYGMIC
                     YCANPGMLSGVTRRYVPRYETLRSSGGKEFKGGIFVATENANIGMFAHDYFHALGGIH
                     EGKRLVP"
     gene            complement(847..1797)
                     /locus_tag="PLR13_11745"
     CDS             complement(847..1797)
                     /locus_tag="PLR13_11745"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQC20056.1"
                     /translation="MDSRRDFIKKSAMVGAAFCLPLFRVEESPAAYPDLKTRSPRKAL
                     ILYYSQTGHTQRYARLIGCLLKGENMTVDEREFQETDQRVLPTYDLVFVGSPVFYYDT
                     PPNVIAWLEQIPSIQGTPVAAFVSFGGPEGNQHNASCHIMKVLAKKGGVPVGRDTFRN
                     VPTYPTPKWDSANQRSGQHLPDAATFDQVRRFTVNTLARISRGETIPVSYELALREGL
                     RALPLIWMNKKVINKHTVNAQKCIRCGTCVRKCPTKAINPFQQTVDRDTCLACFGCLN
                     NCPVGAVVMEYRGEPLYGFFEYLKRNNITIMEPEEFKTRT"
     gene            complement(1936..2265)
                     /locus_tag="PLR13_11750"
     CDS             complement(1936..2265)
                     /locus_tag="PLR13_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQC20057.1"
                     /translation="MFCPKCKRDYAKGIYRCADCGKDLVDRLPQPAMHSSGAEDFEEV
                     FSTYQQGDISFIKSILDGEGITYFFQGESSVMLIAAGAYARLLVKADEAERAREILRD
                     LGLLSGK"
     gene            complement(2293..2835)
                     /locus_tag="PLR13_11755"
     CDS             complement(2293..2835)
                     /locus_tag="PLR13_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQC20058.1"
                     /translation="MKKNFRRYRVTGTTIVFAMLFFLALTAAGANLSFAQSAQDKSTE
                     CYKDIAKTIVHATATGLGEILKDVKSEKEKIALIRSFVNPIRFYPDKSGYFYGYDFQC
                     VNIAHATQKDLPGKNLYDHKDIKGKYVIRELSAAAKKGGGFVDFYWLKPGAKGEQKKL
                     GYVEPIPGTNYFIGTGVYLP"
     gene            complement(2985..3398)
                     /locus_tag="PLR13_11760"
     CDS             complement(2985..3398)
                     /locus_tag="PLR13_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQC20059.1"
                     /translation="MTREQQAALKVAMEFLNHYAERDIESLMDDMATSKPLFMMGTND
                     NEVFKSTRSIRASFKKDFQNMDNIRWGKRRNVHVQADPFLASVIIEMPISYQSAGKKI
                     KTLFRYALTLKKERGKWKICSGMASVPFATGTYSF"
     gene            complement(3497..>3840)
                     /locus_tag="PLR13_11765"
     CDS             complement(3497..>3840)
                     /locus_tag="PLR13_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20060.1"
                     /translation="FIHQTACFENETDAQCINFLYQAMHTPKWYPTVGELKERLSEAH
                     WEVIDEHPAPPLKLSSAELGRRYGLNMPMMESISRKLLDQFGNIDHVFQYGENEFVAH
                     LHYRIFAVRAI"
BASE COUNT          956 a          982 c          890 g         1012 t
ORIGIN      
        1 gacatgatgt cccacggtcc catatagatg gcatgatgtt cgaacgaagg atttgcggcg
       61 gatgcgtcag actgacgcac gaagtcgtaa agtcagggca ctaatcgttt tccttcgtga
      121 atgccgccga gagcatgaaa atagtcatgg gcaaacatgc cgatattcgc attttccgtt
      181 gcgacaaaaa tgccgccctt gaactcttta ccgcctgacg atctcaacgt ttcatagcgg
      241 ggcacatacc gtctggtgac tcctgaaagc atgccgggat tggcgcagta acagatcatc
      301 ccgtatccct tgccgggcat ggtcgtgact gccggaacga tcaggatatg gtcgtacgcg
      361 gaaaaatcga ctctgctttc caatgccgtc atggtgtctt caatgagttt gcgaacccgt
      421 cttctgtcca cgcggaaatt atagggactg atgttgtagc tggcgatcga atcgggcagc
      481 atgacgtatc ccataaaatc ggccttgatg gaagcgagtc cgtacgattg ctctttgaca
      541 taagcgttga gtccgcccac aacctttctc cggacggctt caatcggcag ggacggtgtt
      601 gcgtccggaa accggaccac aaccgtcagg acgttttttt caccgagggc ttgtgttgct
      661 tgcggaccga gaccatcgtt caaactttcg gctcgaatgg cgccggcaaa gataccggac
      721 atggacaaaa gaatgatggc cagcctgacg atcatttgtg tgcttttcat tgcaggacac
      781 ctctcttcct ctagcgacaa tgaacaattc tattttcctt acagacacag catccttaat
      841 aatacatcag gtgcgggttt tgaactcttc cggctccata atggtaatgt tgttgcgttt
      901 cagatattca aaaaagccgt ataaaggctc gcccctgtac tccataacca cagcgccgac
      961 agggcaatta ttcagacacc cgaaacaggc aaggcacgta tcacgatcaa cggtttgttg
     1021 aaagggattg atggctttgg tcgggcattt tctgacacag gttccacacc ggatacattt
     1081 ttgtgcattg acggtatgtt tattgatgac ctttttattc atccagatca gaggcaacgc
     1141 gcgcaatcct tcccttaagg ccagttcata acttaccgga atcgtttccc cccgggaaat
     1201 tcttgcgagc gtattgacgg taaaacgccg gacctgatcg aaggtggccg catcgggcag
     1261 atgctgtccc gatcgctggt tggcgctgtc ccattttggc gtcggatagg tcggaacgtt
     1321 tctgaacgta tccctcccga cgggaacccc gcccttcttc gccagtactt tcattatgtg
     1381 gcaggaagcg ttatgctgat tgccctccgg accgccgaag gacacaaatg ccgcaaccgg
     1441 cgtgccctga atggaaggga tttgttccag ccatgcgatg acattgggcg gcgtgtcata
     1501 atagaagacc ggcgagccga caaaaacaag atcgtacgtc ggcaaaacac gttgatcggt
     1561 ttcctgaaat tcccgttcat cgaccgtcat attttccccc ttcaggaggc aaccgatcag
     1621 gcgggcatac ctttgagtat ggccggtctg gctgtagtat aaaatcaggg cttttcgggg
     1681 agaacgggtt tttaaatccg gataagccgc cggactttct tcaacccgga aaagcggcag
     1741 gcagaaagcg gctccgacca tggcactttt tttgataaaa tctctgcgtg aatccatgat
     1801 tcctctcgct acaggttgct tcaataaaaa tggatatgcc ctttaaaatt cttcaccgcc
     1861 gcaataattt tcccgtccgg catatcccgg accgataaaa gattgcgaga ttttttccgg
     1921 ggaaactatc atccatcact tgccgctcag caatcccaga tcccgtaaaa tttcccttgc
     1981 ccgttccgct tcgtcagctt tcacgaggag acgggcatag gcgcccgccg caatgagcat
     2041 cacagaactt tccccctgga aaaaatacgt gatgccttca ccgtcaagga tggatttgat
     2101 gaaggatata tcaccctgct gatacgtgga aaacacttct tcaaaatctt ccgcgccgga
     2161 tgaatgcatt gccggttgcg gcagacgatc gaccagatcc ttaccgcaat cggcgcaccg
     2221 atatatccct ttcgcgtagt cgcgtttaca tttcggacaa aacatctcta ctccatacgg
     2281 aagtcaatcg gcctacggca gataaacgcc ggtgccgatg aaatagtttg ttccgggaat
     2341 cggttcaaca taacccaatt ttttctgttc gcctttggcg ccgggcttca accagtagaa
     2401 gtcgacaaat cctccgcctt tcttggccgc tgcgctcaat tcgcgaatca cgtatttccc
     2461 ttttatatct ttatgatcat aaagattttt ccccggcaaa tctttttgtg tcgcatgagc
     2521 aatatttacg cactggaaat catagccgta aaaatacccc gatttatccg gatagaagcg
     2581 gatgggattg acaaaagaac ggatcaacgc aattttttct ttttccgatt tgacatcttt
     2641 taagatttct cccaatcccg tagcggtcgc atgaacgatt gttttcgcaa tgtctttata
     2701 gcattccgtt gatttatcct gagcgctctg cgcgaaagaa agattcgcac cggcggcggt
     2761 cagcgccaga aaaaataaca tggcaaacac gatcgttgtt ccggttacgc gataacgtct
     2821 gaaatttttt ttcattatga caattcctcc tgaattaagt tgttaaatgt caagtgttgt
     2881 gaactttcaa ttaacgcccc atgaacagac ggctcctgat gacggatgtt tccgcaatcc
     2941 ccccgtcatc ggaaaacatg aacgagccgc tcggtatttc ccggctaaaa agaataggtc
     3001 ccggtcgcga acggaacgct cgccattccg gaacaaatct tccatttacc gcgttccttt
     3061 ttaagcgtca gagcatagcg aaaaagcgtc ttaatttttt ttccggcgct ttgataggaa
     3121 atcggcatct cgatgatgac gctggccaga aagggatcgg cctgaacatg gacattgcgt
     3181 cgtttacccc aacggatgtt gtccatgttt tgaaagtctt tcttgaaaga ggcgcgaata
     3241 cttctggtcg atttaaaaac ttcgttgtca ttggtgccca tcatgaataa aggctttgaa
     3301 gttgccatgt cgtccattaa gctctcaata tccctttcgg cataatgatt aagaaactcc
     3361 atcgcgactt ttaatgcggc ttgttgttcc ctggtcattg ccaacctcct tttgtaacgg
     3421 ttaaaatccg gttaaaatat gcctgcgtca tttccataat catctatggt catgcggtaa
     3481 aacgccgaag cgcccatcaa atcgcccgga cggcaaaaat ccgataatgg agatgagcca
     3541 caaactcatt ttccccgtat tgaaaaacat gatcgatgtt gccgaattga tccagcagtt
     3601 ttcgggagat gctttccatc atcggcatat tcagcccgta gcgccgaccg agttcagcgg
     3661 aggaaagttt cagcggcggc gcagggtgtt catcaatgac ctcccagtgc gcttccgaca
     3721 atctttcttt aagctccccg acggtcggat accatttggg cgtatgcatc gcctgatata
     3781 gaaaattaat gcattgcgca tccgtttcat tttcaaaaca tgctgtctga tgaataaata
//
LOCUS       DAONQZ010000131         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_24629_length_3790_cov_16.873628, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000131 DAONQZ010000000
VERSION     DAONQZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24629_length_3790_cov_16.873628"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLR13_11770"
     CDS             <1..294
                     /locus_tag="PLR13_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HQC20061.1"
                     /translation="PKLIENGLRGMIGKGNRSPEVVEAIKKYKSVYFGAIGGIAALMS
                     QCVKKVEMVAYEDLGPEAIRKLTVRDFPLVVVNDSSGKDLYVSAKQKWHISLI"
     gene            393..941
                     /locus_tag="PLR13_11775"
     CDS             393..941
                     /locus_tag="PLR13_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQC20062.1"
                     /translation="MIEVRWHGRGGQGAVTSVELLAQAAIAAGKYAQGFPSFGPERRG
                     APVAAFTRVADKKINVRSGIYEPDVVLVLDSSLIGLVNVLDGLKPGGKLIVNTHKTPE
                     EIRKALDYKGILAIVDGTGIARKEMGVPIANTTMIGALLKVAGIMELDAMKESIEHRF
                     GRIAQKNLNAMKRAYDEITIYN"
     gene            955..1233
                     /locus_tag="PLR13_11780"
     CDS             955..1233
                     /locus_tag="PLR13_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQC20063.1"
                     /translation="MALKTWKELPIGCVVSEPGSASEYHTGSWRSQRPIWDNAKCIKC
                     GICYVYCPEGCVQQTEDGFFQADLDYCKGCGICAHECWPRAIKMLEEE"
     gene            1235..2410
                     /locus_tag="PLR13_11785"
     CDS             1235..2410
                     /locus_tag="PLR13_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQC20064.1"
                     /translation="MGKRIGMEVAIAVAEAVGLCDIDMAAVYPITPQSHIAEHLSDLV
                     ANGEIDAEFVTVESEHSAMSAVIGASGAGARAFTATSSQGLMLMHELLPIASAMRLPI
                     CMAIANRAVSGPLNILNDHTDIMPQRDSGWISLFVENGQEAIDMSIIAFKIAEHKDVM
                     LPVNVNIDGFQLTHMVEPFEMPSKEEVDRFLPPFVPHATLHPAKPVTMGAFAMSDYFT
                     EIMKAKDEALKNSKKVIANVLDEWGKMFGRNYKLVETYKSEDAEVIMLTMGSMGETAQ
                     MAIDDLRAKGVKAGLVKLRLWRPFPFEEIKAAIKNAKKLIVTDRAISFGGPGGPVFSE
                     IKSAMYAATQRPLIYNYIYGLGGRDVTVGDFIGMFEKVLADTEHKAVDTYEFWGVRE"
     gene            2410..3360
                     /gene="porB"
                     /locus_tag="PLR13_11790"
     CDS             2410..3360
                     /gene="porB"
                     /locus_tag="PLR13_11790"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HQC20065.1"
                     /translation="MNIVENFDLFAPRLINKQELISSGHRACSGCAEVLAVRLMCKAL
                     GENTVIASATGCMEIISSMFPTTAWRVPWIHVAFENTAAVASGIEAGLKALRRKGKID
                     DRYVKVVGMAGDGGTMDIGIQALSGAMERGHDMMFVCFDNEAYMNTGIQRSSGTPMGA
                     STTTAPAGKVSSGQKTWKKNMAEIMVAHEIPYVATVCPSYPLDFIRKIEKAKSIKGPS
                     YIHCFSMCPTGWRSPSHTAISMGRLAVETGVFPLFEVEHGHYRVSPDMPKKLRPIREY
                     FKPQGRFRHLKDNEIDQIQERVSMEYQKLMEKAKYLKTGN"
     gene            3399..>3790
                     /locus_tag="PLR13_11795"
     CDS             3399..>3790
                     /locus_tag="PLR13_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQC20066.1"
                     /translation="MTKASSIAAKTKEIVKKHGKDKSALIAVLQDIQESFNYLPKDAL
                     KTAGKALGVPFSRVYEVATFYTAFSLKPRGEHIVKICMGTACHVRGAAAIQDKMERTL
                     CIKPGETTPDNKFSLETVNCVGACALGPV"
BASE COUNT          995 a          836 c         1050 g          909 t
ORIGIN      
        1 ccgaaattaa tcgaaaacgg ccttcgggga atgatcggca agggcaaccg ttcgccggaa
       61 gttgtcgaag ccatcaaaaa gtacaaatct gtttatttcg gagcgatcgg cgggattgcc
      121 gctttaatgt ctcaatgcgt caaaaaagtc gaaatggttg cctatgaaga cctcggtccc
      181 gaggcgatca gaaaactgac agtcagagat tttccgctgg ttgttgtcaa cgattcctcg
      241 ggcaaagacc tgtatgtttc ggcaaagcaa aaatggcata tctctttaat ataactagct
      301 atttcagcgt tcgtgaaaat ctgttgactg aaatttgctt ctatattatg aatgatggtc
      361 atttttgtat cacgataagg agacgaaaat aaatgataga ggttagatgg cacggacgtg
      421 gcggtcaggg agcggtgacc tcggtggagt tgctggctca ggcggcaatt gcagccggaa
      481 aatacgctca gggatttccc agtttcggtc cggaaagaag aggggcgcct gtagccgcat
      541 ttacccgtgt tgccgataaa aaaatcaatg tccgctcagg catctatgag cctgatgtgg
      601 tgctggtgct ggattccagt ttgatcggtc tggtgaatgt tctcgatggt ttgaaaccgg
      661 gcggcaaatt gattgtcaat acgcataaaa ctcctgaaga aattcgcaag gcgcttgatt
      721 acaaggggat attggccatt gttgacggca ccggaatagc acgtaaggaa atgggcgtgc
      781 ctattgccaa caccaccatg atcggcgctc ttttgaaagt ggccggcatt atggaactgg
      841 atgcgatgaa agaatctata gagcaccgtt tcggcagaat cgctcagaag aatctcaacg
      901 cgatgaagcg ggcttacgac gaaataacaa tatacaatta aggggtagat ctcaatggca
      961 ttgaaaacat ggaaggaact tcccattggc tgtgttgtgt ccgaacccgg aagcgccagt
     1021 gaatatcata cgggaagctg gcggtctcaa agacccatct gggataatgc aaaatgcatc
     1081 aagtgcggca tttgttatgt ttattgtccg gaaggttgcg tgcagcagac cgaggacggc
     1141 ttctttcagg ccgatctgga ttattgcaaa ggttgcggta tttgtgcgca tgagtgttgg
     1201 ccccgcgcga taaagatgtt ggaggaggag taatatggga aaacgcattg gaatggaagt
     1261 ggcaattgcc gttgcggaag ccgtgggtct ttgcgatatt gacatggccg ccgtttatcc
     1321 tattacgccc cagtcgcaca tcgcggaaca tctttccgat ctggttgcca acggtgaaat
     1381 tgacgcggaa tttgtcaccg ttgaatccga gcattcggcg atgagcgcgg tgatcggcgc
     1441 ctccggcgca ggtgcccgtg cgtttacggc aacaagttct caggggttga tgctgatgca
     1501 cgaacttctt cccattgcgt cggcgatgcg cctgccgatt tgcatggcaa ttgccaaccg
     1561 cgcggtgtcc ggtcccctga acatattaaa tgatcacacc gatatcatgc cccagcgcga
     1621 ttcgggctgg atttctcttt ttgtggaaaa cggccaggaa gcgattgata tgtcgatcat
     1681 tgctttcaaa attgccgagc acaaggatgt catgctgccg gtcaatgtta atatcgacgg
     1741 tttccagttg acgcacatgg tggagccttt tgaaatgccc tcaaaggaag aagtggatag
     1801 atttctgccg ccgtttgtcc cgcacgcaac gctgcatccc gcaaaaccgg ttacgatggg
     1861 cgcttttgcc atgtcggact attttacgga aattatgaag gccaaggatg aggcgctgaa
     1921 aaattcaaag aaagtcattg caaatgtatt ggacgaatgg ggcaaaatgt ttggccgcaa
     1981 ttacaaactg gtggaaacgt ataaatccga agatgccgaa gtcattatgc tgaccatggg
     2041 ctcgatgggt gaaaccgcac agatggctat tgatgacctt cgtgccaaag gcgtgaaggc
     2101 cggtctggtg aaattaagat tgtggcgtcc ctttccgttt gaggaaatca aagccgcgat
     2161 taaaaacgcg aaaaaactga ttgtgacgga tcgtgccata tcgtttggcg gccccggcgg
     2221 tccggttttt tcggaaatca aatccgccat gtatgcggca actcagcggc ctctgatata
     2281 caattatatt tacggactgg gtggtcgcga cgtgacggtg ggcgacttta tcggcatgtt
     2341 tgaaaaagtt ctggcggata cggaacataa agcagtggat acctatgaat tttggggagt
     2401 gagagaataa tgaatatcgt agaaaatttt gatttatttg ccccgcggct gattaataaa
     2461 caagaactga tctcttccgg tcatcgcgcc tgttcgggtt gtgctgaagt tctggctgtg
     2521 agattgatgt gtaaggcgct tggtgaaaat acggttatcg cttcggcgac cggctgtatg
     2581 gaaatcatct ccagcatgtt tcccacaact gcctggcgcg tgccttggat tcatgtggct
     2641 tttgaaaaca cggcagccgt tgcatccggt atagaagccg gactcaaggc attgcgcaga
     2701 aaaggaaaga tcgacgaccg ttacgtcaag gtggtcggca tggcgggcga cggcgggacc
     2761 atggatatcg ggattcaggc cctttccgga gcgatggaac gcggtcatga tatgatgttc
     2821 gtctgtttcg acaatgaagc gtacatgaac acgggtattc agcgttccag cggtacgccg
     2881 atgggcgctt cgacgaccac ggcaccggcc ggcaaagtga gttccggaca gaaaacatgg
     2941 aagaagaaca tggcggaaat catggtcgcc cacgagatac cgtatgtcgc aaccgtttgt
     3001 ccgagttacc cgctggattt tatccgcaag attgaaaagg cgaaaagtat caagggaccg
     3061 tcctatatcc attgtttctc tatgtgtccg accggctggc ggtctccgtc gcatacggca
     3121 atatccatgg ggcggctggc ggtggaaacc ggcgtatttc ctttgtttga agtggagcat
     3181 ggccattacc gggtttctcc ggatatgccc aaaaaactca gacccatccg ggagtacttc
     3241 aagccgcagg ggagattccg tcatttgaaa gataatgaaa ttgatcagat tcaggaacgc
     3301 gtttccatgg aatatcaaaa actgatggaa aaagccaaat atctgaaaac aggcaattaa
     3361 gaggaatgtc ctcaaaagaa taccgaggtg aaaaaagcat gacaaaagct agcagcatcg
     3421 cagcaaaaac caaagagatt gttaaaaaac acggtaaaga taaaagtgca ctgattgccg
     3481 tcttgcagga tattcaggag tcctttaatt acctccccaa ggatgctctg aaaacggccg
     3541 gcaaagctct gggcgttccc ttcagccgcg tttacgaagt tgccacgttt tatacggcct
     3601 tcagtctgaa accgcgcggc gagcacatcg tgaaaatctg catggggacc gcctgtcatg
     3661 tgcgcggcgc ggcggccatt caggataaaa tggagcgaac attatgtatc aaaccgggcg
     3721 agacaacacc ggacaataaa ttctctctgg aaaccgtcaa ctgcgtgggc gcctgcgcgt
     3781 tggggcctgt
//
LOCUS       DAONQZ010000132         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_25149_length_3731_cov_18.278020, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000132 DAONQZ010000000
VERSION     DAONQZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25149_length_3731_cov_18.278020"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..834
                     /locus_tag="PLR13_11800"
     CDS             163..834
                     /locus_tag="PLR13_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC20067.1"
                     /translation="MPKKPRTKEEIENIREKILDTALHIIIEEGFNNLSLRKIASRLN
                     VTATTIYNYYTNKDEINLMIRIRGFEKLHDLLIARSAALKKIEDRLKAMIRAYIEFGL
                     TYPSYYDIMFNLHTPKYLDYAGTEIEPLAFREKQNALKCLTLFIDPVTSYIPIKGKKK
                     EHFILYQVIKFWSDLHGLITLNNSRLFHEVTDDVDDFIAKRTADIVDNFLKLKDSLDE
                     GKKIF"
     gene            868..>3731
                     /locus_tag="PLR13_11805"
     CDS             868..>3731
                     /locus_tag="PLR13_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQC20068.1"
                     /translation="MQNVQPVQKSYANTLARMFFKFVALNFNNRPSLNRYLKSDDGWI
                     NFTFGIKTENGSVEQAMRFENGKVTVLKNIPETLDSQLIFVDENALKEAATQPPNKLM
                     LALMENRMITIGNLGYLQLINLYLSLLVKQIQIAKLKKEAKTENRKYDAAKASRYCIE
                     ARKPRQIKAVNVDPGVRFLNDPYLAQYSLDDFPRVKEFLDIHLKIKPAICHERAELVT
                     QWFKENGFEKDKNGKPWVSELRQAQAFQYLMANKKPIIRKNDLIAGTTTTKDIGVIIY
                     PEAHGTMIWGELLTVPYRPLNPYNIDADTLDVLHNKVFPFWLKRNFREWVRDHKDYPL
                     CQKLDERFAACFLWKTVALSHTILNYPEVLSLGTSGIISKIDKELSETPESEKQKRDT
                     LQAMKISLEGMNAYAGNLAQEAWRLASQETQSVRKNELLHLAEICKRVPAHPCTTLDE
                     AVNALWIIWVGVHMENTNAGFSLGRLDQWLQPYFTADMKKIQDEKERQEYVKHAIELI
                     GCFYMRCTDHLPLIPDIGNYLFGGSSSDQAITLGGVTPDGEDAVNDMTYIFLKVTEML
                     SIRDPNVNARYNKSKNSATYLKRLCEVNINTTATPSIHNDEAVMASLAEFNYPPEHLR
                     DWSATGCVEPTLSGRHIGHTNCMMFNMVAALEMALNNGRHPLMRWDLGPKTGDIRAGA
                     FPDFESFFNAFAAQLRFLADQTCEYNNLLGEAHQHIRPTPFISALIEGPLKKGKDVTK
                     GGARYNSSGTACIGLADITDSLMVVKKLVFEEKKITFERLLDAVNTNFRNDSALLAQI
                     KNKIALFGSGNEEALATANRVIKLAHDIFGEHTNYRNGKYTAGFWSMSNHVAFGTLTG
                     ALPSGRLAGKAFTPGLTPEPHASKNILDNIRDVAKLKAENMNNNIAFNVKVVPGAQES
                     HQKVVDNIYSYVKTYSDLGGMQMQLNVVSSKTLRDAMEHP"
BASE COUNT         1091 a          940 c          843 g          857 t
ORIGIN      
        1 ttccccagcc ataacttcac catttaatat cagttatttc aatattttac atgaatatta
       61 tgcggccggg caatttctgc cggtcccgtt gatattgcgc ttgacttttt aaaataacac
      121 tgttaagtta acatcgtaat atttaaaaat aagaatcaaa ttatgcccaa aaaaccgaga
      181 acgaaagaag aaatagaaaa tattcgggag aagatcctgg atacggctct tcacattatt
      241 attgaggaag gatttaataa tctcagtctt cgcaaaatag cttcccgttt gaatgtcacg
      301 gccacgacca tctacaatta ttataccaat aaagatgaaa tcaatctgat gatcagaatt
      361 agaggctttg agaaacttca cgacctcctg atcgcacgct cggctgcctt gaagaaaata
      421 gaagacagat taaaagccat gatccgtgcc tacatagaat tcggcctcac ctatcccagc
      481 tactacgaca tcatgttcaa tcttcatacc cccaagtatc tggactatgc gggaacggaa
      541 atcgaaccgc tcgcctttcg ggaaaagcag aatgcgttaa aatgtctcac gctctttatt
      601 gaccccgtca cgtcatacat tcccatcaag ggaaagaaaa aggagcattt tattttatat
      661 caggtaatta aattctggtc tgaccttcac ggccttatca cgttgaacaa cagccgcctc
      721 tttcacgaag taacggatga tgtggatgac tttattgcaa aacgaacggc ggacatcgta
      781 gacaatttcc tgaaactgaa ggacagcctg gatgaaggaa agaaaatttt ttaactgatc
      841 attaaacccg ttattcggag gtctattatg caaaatgtcc agcctgtgca aaaaagttat
      901 gccaacaccc ttgcccgcat gtttttcaaa ttcgttgcct tgaatttcaa caaccggccg
      961 tcgctgaacc gatatctgaa aagcgacgac ggctggatta attttacatt cggcatcaaa
     1021 acggaaaacg gcagcgtcga gcaggccatg cgtttcgaaa acggcaaggt taccgtgttg
     1081 aaaaatattc cggaaaccct ggattcgcaa ttgatattcg tggatgaaaa cgcccttaag
     1141 gaagccgcca cgcaaccgcc caacaaattg atgttggcgc tcatggaaaa cagaatgatc
     1201 acgattggga acctcggcta tctgcagttg attaatttgt atctttcgct ccttgtgaaa
     1261 cagatccaga ttgcaaaact caagaaagaa gccaagacag aaaaccggaa atacgatgca
     1321 gccaaagcat cccgatactg tatcgaagcc agaaaacccc gccagatcaa ggccgtcaac
     1381 gttgatccgg gcgtccggtt tctaaacgac ccctatctgg cccaatattc ccttgatgat
     1441 tttccccggg tcaaagaatt tctcgacata cacctgaaaa taaaaccggc catatgccac
     1501 gaacgggcgg aacttgtcac acaatggttt aaagaaaacg gatttgaaaa agataaaaac
     1561 ggcaaaccct gggtttccga actgcgccag gcgcaggctt ttcaatatct gatggcaaac
     1621 aaaaaaccaa tcatcaggaa aaatgatctg atcgccggaa caaccaccac gaaagacatt
     1681 ggcgttatta tctatccgga ggcgcacggc accatgatct ggggagagtt gctcacagtt
     1741 ccatacaggc cgttgaaccc gtataatatt gatgccgata cgcttgacgt tctgcacaac
     1801 aaagtctttc cgttctggct caagcgcaac ttccgcgagt gggtgcggga ccataaagat
     1861 tatcctcttt gccagaagct tgatgagcgt tttgccgcct gctttttatg gaaaaccgtc
     1921 gcgctgtcgc atacgatttt aaattatcct gaggtcttaa gcctgggcac cagcggtatt
     1981 atctcgaaga tagataaaga attaagcgaa acacccgaat cagaaaaaca aaaacgtgac
     2041 acgctgcaag ccatgaaaat cagtcttgag ggaatgaatg cctacgcggg taatctcgcg
     2101 caagaagcgt ggcggctggc gtctcaggaa actcagtctg tccgcaaaaa cgaactgctg
     2161 catctggccg aaatctgcaa gcgcgttccg gcccatccct gcacaacgct ggatgaagcg
     2221 gtcaatgccc tgtggatcat ctgggtcggc gtccacatgg aaaacacgaa cgccggattt
     2281 tctctcggaa gactggatca atggcttcag ccttatttta cggccgacat gaaaaaaatt
     2341 caggacgaaa aagaaagaca ggaatatgtc aagcatgcga tagaattgat tggatgtttt
     2401 tacatgcgct gcacagacca cctgcccctg attccggaca tcggcaatta tctgttcggc
     2461 ggctcctcct ccgatcaggc cattacgctg ggcggcgtaa cacccgacgg cgaagacgcc
     2521 gtcaacgata tgacctacat ttttctgaaa gtgacggaaa tgctttccat ccgcgatccc
     2581 aacgtcaatg cccgttacaa caagagcaaa aacagcgcga cctacttgaa aaggctttgc
     2641 gaagtcaata tcaacacaac ggcaaccccg tccattcata atgatgaggc ggtcatggcc
     2701 tcgcttgcgg aattcaatta tccgccggaa catttacgcg actggtcggc aaccggctgc
     2761 gtggaaccga ccttgtccgg acggcatatc ggtcacacca actgcatgat gtttaatatg
     2821 gtggccgcgt tggaaatggc cctcaacaac ggccggcatc cgttgatgcg ctgggatctg
     2881 ggaccgaaaa caggcgacat ccgcgccggc gcattccccg attttgaatc cttcttcaac
     2941 gcgtttgcag cgcagctccg ttttctggcc gatcaaacct gtgaatacaa caatctgctg
     3001 ggcgaagctc accaacacat ccgcccgact ccttttatct cggcgctgat cgaaggtccg
     3061 ttgaaaaaag gaaaagacgt aaccaagggc ggggcccgtt acaattcatc cggaacggcc
     3121 tgcatcggcc ttgccgacat taccgactcg ctgatggtgg tcaaaaagct ggtttttgaa
     3181 gagaagaaga taacgtttga gcggctgctg gatgcggtca atacaaattt cagaaacgac
     3241 tccgctctgc tggctcagat taaaaacaag attgctcttt tcggatcagg caatgaagag
     3301 gcgctggcaa cagccaatcg cgtcatcaaa ctggctcacg atattttcgg agaacacaca
     3361 aattaccgca acggcaaata cacagccggt ttctggtcga tgtccaatca cgttgctttc
     3421 ggtacgctga ccggcgcgct tccgtcagga cggctggccg gcaaagcctt cacaccgggt
     3481 ctcaccccgg aaccccatgc gtcgaagaat attctggaca acatccggga tgtggccaaa
     3541 ctcaaagcgg aaaacatgaa caacaatatc gctttcaatg tcaaagtcgt ccccggtgca
     3601 caggagtcgc atcagaaagt cgtggataat atttattcct acgtcaaaac ctacagcgat
     3661 ctgggcggta tgcagatgca gttgaacgtc gtgtcgtcaa aaacgcttcg cgacgccatg
     3721 gaacatccgg a
//
LOCUS       DAONQZ010000133         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_25356_length_3708_cov_14.479058, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000133 DAONQZ010000000
VERSION     DAONQZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25356_length_3708_cov_14.479058"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..3261)
                     /locus_tag="PLR13_11810"
     CDS             complement(172..3261)
                     /locus_tag="PLR13_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQC20069.1"
                     /translation="MPLPNLISLLFFLAMVVYFFLGVYVIRLNFGSVIHRLFFCICLT
                     LCIWSLSFSIANSAMSYEMSLTWRRIGAIGWGTLYSFLLHYTLCLTGKQNVLKKRWIY
                     ALIYLPAILNIFVFSLYGDLALKRYVLEYTYAGWVNVRPASAWDAVFNVYYAVFSLIV
                     IGLFFHWGWSSKERVIKKQAYLIIVSYAVALVVGTMTEFVINYYFPFKFPQLAPLIIL
                     IPIATMYYCIAKYGLMAPKLKNTVPQEGQILSEASQSELYQYLTVAYILSAFLHFAIE
                     TFIQGSPLITSLPKTLLLIVLGLALQIIQKLNIQQGAKDLLLNVLVILSIPLIVLSFV
                     SDHVTFWSLPLIFVMVSILFRQKRLLYVVSLVTFLTLLWIWITVPEQTVKIDASNHIF
                     RIGIFGVLIWIAFYINRIYFNRLAKNEEQVKHQSLLADISAQFVDASQENIDKIINDM
                     LRQCGRHFQADRAGLFMYSGKNHQPVHSHEWCNDGIASTLDSKNQQTVLELPGVKDQI
                     DKAGYIHVSDAETLQPGAIPDKDSLKDKQIQSLIIVALTGTDNVIGYIFFEAIQSPRK
                     WDQDHREIIQVLGNLLSDALRQVEAEKAINYMAYYDALTALPNRALLTDRLKQAISLA
                     MRTEKLISIIFIDVDSFKSVNDTMGHDGGDAMLMQIAERLSACVRQYDSVARFGGDEF
                     LVMLQQIPDIKYIGESANKIMSVFNEPITIKGQEFFITASAGVAVFPFDGEDADELIK
                     NADMAMYAAKSTGKNKVTFCSAEMKKEVLDNMELTNSLYRAVGRNELFLLYQPQVNPD
                     TIEIIGLEALIRWRHPVKGIIPPTKFIPIAEKTSLIHSIGEWVLMTACRQNMEWQNQG
                     LKPTRIAVNLSVKQFHSPNIVNVVDRVLRETKLMPCYLELEVTESLGTHDKDYVINTM
                     NKLKALGLSLSIDDFGTDYSSLGRLKDLPVDRIKIDMQFIRGIAKNAKDEGIIKIILQ
                     LGKTLGLHVVAEGVETQQQLAFLKDNLCFEIQGFYFHKPVVAEDIVPILRRQEKGNH"
BASE COUNT          987 a          844 c          846 g         1031 t
ORIGIN      
        1 cgcaggtcag aatcagaata gataccacta attttttcat gggtttatcc tccttaaaaa
       61 agcggaaaat ttgatgttgc aagttcaata gttagttgtg attaaagcag aaccgccccc
      121 ttttattttt gcccaattct tttttcatat gacagctttt aatgaatgaa atcagtggtt
      181 gcctttttct tgcctacgca agatgggcac tatatcctct gcaaccactg gtttgtggaa
      241 gtagaatccc tgaatttcga aacataaatt gtctttgaga aaggccagct gctgctgggt
      301 ttccactcct tcagccacga catgcaggcc aagcgttttc ccaagctgta aaatgatttt
      361 gatgattcct tcatccttgg cgttcttggc gattccgcgg atgaactgca tgtcaatttt
      421 aatccggtca accggcaggt ctttcaggcg gcccagcgag gaataatcgg ttccgaaatc
      481 gtcaatcgag agagacagcc ccagcgcttt gagtttgttc atcgtgttga tcacgtagtc
      541 cttgtcgtgt gtccccaggc tttctgttac ttccagctcc aggtaacagg gcatcagttt
      601 cgtttcgcgt aacaccctgt cgacaacatt gacgatattt ggactatgga actgttttac
      661 tgaaagattg acggcaatcc tcgttggttt gaggccctga ttctgccact ccatgttctg
      721 gcggcacgct gtcatcagca cccactcgcc aatggaatga atcagacttg ttttttccgc
      781 tatgggaata aactttgtcg gcggtatgat tcctttcacg ggatgccgcc agcggatcag
      841 ggcttccaac ccgatgattt caatggtatc aggattgacc tggggctgat aaagtaaaaa
      901 cagttcgttt cttcccacag cccggtacag gctgttggtg agttccatgt tgtctaaaac
      961 ttcctttttc atttcggcgg aacaaaaggt gactttgttt tttcccgtgc ttttggcggc
     1021 atacatggcc atgtccgcgt ttttaatgag ttcgtccgcg tcttcaccat cgaatggaaa
     1081 tacggcgact cccgcgctgg cggtgataaa aaactcctgg cctttgatgg tgatgggctc
     1141 attaaaaacg ctcatgattt tgtttgcgga ttctccgatg tatttaatat cggggatctg
     1201 ctgaagcatc acgagaaatt catctccgcc gaaacgcgcg acactatcat attggcgcac
     1261 acatgccgac agcctttccg cgatctgcat gagcatggcg tctcccccgt catgtcccat
     1321 cgtatcgtta acggacttga aagaatcaac gtcgataaag atgatgctga tgagcttttc
     1381 agtgcgcatg gccagactga tggcctgttt caggcgatcc gtcaaaagcg cgcgattcgg
     1441 caatgcggtc aacgcatcat agtaagccat atagttgatt gctttttcgg cttccacctg
     1501 gcgcaaggca tccgataaaa gatttcccaa gacctgaatg atctcccggt ggtcttgatc
     1561 ccacttccgg ggactttgta ttgcctcaaa gaaaatatac ccgataacat tatctgtgcc
     1621 ggtcaatgcg acaatgatta aggattggat ttgtttgtct ttcagggagt ccttgtccgg
     1681 tatggcaccg ggttgcaatg tttctgcgtc ggaaacgtga atataccctg ccttgtcaat
     1741 ctggtcttta accccgggca gctccaggac tgtttgctgg tttttggagt ctaaggtgga
     1801 ggcgattccg tcgttgcacc actcatggga atgcactggc tgatggtttt ttcccgaata
     1861 catgaaaaga ccagcccggt cggcctgaaa atgacggccg cactgacgca gcatatcgtt
     1921 aatgatctta tcgatgttct cttgactggc atcaacaaat tgggcggaaa tgtcagccag
     1981 caaactctgg tgtttgacct gttcttcatt ttttgccaac cggttgaagt aaatgcgatt
     2041 gatgtaaaat gcgatccaga tcaaaacgcc gaaaatgcct attcggaaga tatggtttga
     2101 tgcgtcaatc tttacggttt gttccggcac cgttatccat atccataata gcgttaagaa
     2161 agtcacaagg cttaccacgt acaataatct tttttgacgg aataaaatag ataccatgac
     2221 aaaaataagg ggcaaggacc agaaagtaac gtgatcgctg acgaagctca gaacaataag
     2281 aggaatcgat aagattacca ggacattcag caacaaatct ttagcccctt gttgaatatt
     2341 taatttttga ataatctgca gcgccaatcc cagaacgatc agcaacaagg ttttcggcag
     2401 cgatgtaatt agggggcttc cttggatgaa cgtttctatg gcgaaatgca aaaacgcact
     2461 caggatatag gccacggtta aatactggta aagctcggat tggctcgctt cgctcaggat
     2521 ctgaccttcc tgaggaacgg tgtttttcaa ttttggtgcc attaaaccgt atttggcgat
     2581 gcaataatac atcgtcgcta tcggaatcag gatgattaag ggggcgagtt ggggaaactt
     2641 gaatggaaag taataattga ttacaaattc ggtcatcgtt ccgacaacca gagccaccgc
     2701 ataagaaacg atgatcaggt acgcctgctt cttgattacg cgttcttttg aggaccagcc
     2761 ccagtgaaaa aacagaccga taacgatcaa agaaaaaacg gcgtaataaa cattgaacac
     2821 cgcgtcccac gcggacgcag gccggacatt cacccaaccg gcataggtgt attccagaac
     2881 gtatcttttt aacgccaggt cgccataaag gctgaagaca aagatattca agatggccgg
     2941 aagatagatc agcgcgtaaa tccatctttt cttcaaaacg ttttgctttc cggtcaggca
     3001 aagcgtgtag tgaagaagaa aactgtataa tgttccccat cctattgcgc cgatgcgtct
     3061 ccaggtcagg ctcatttcat aagacattgc cgagttggcg atggagaaag aaagtgacca
     3121 gatacaaagg gttaagcaga tacaaaaaaa gagccggtga atgacagagc cgaagttgag
     3181 cctgatcaca taaacgccca ggaaaaagta cacgaccatg gcgaggaaaa acagcagtga
     3241 aattaagttc ggtaaaggca ttttatggaa ttcctgtagc gatcaatttc gagaatccat
     3301 ctatttttct attcaattgg aatttacaat taaaatagaa cggcctgctt tatttttcaa
     3361 tccctttttg tatcgaacat cgcgactcat ccttgagacg actccgtcgg cggtggtgcc
     3421 gaggaagatg gggttgccgg tgcggtaatt atccttcttt tttccggttg ttttctcgcc
     3481 atcttgtctt ggccaaacat tgtttatatg gatcggttct aatgccgcaa acgccgaacg
     3541 cagcgttgtc ctaaaaatca gccactaaat atgttgtttg atatctcatg gctttaaatt
     3601 aaataataat aaatccgtat tatttttcct ccagcccctt aacgaacgcc agaacatttt
     3661 ttcccagcac actatgatta tagccgcctt ctaaaatgcc gaagcagc
//
LOCUS       DAONQZ010000134         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_25588_length_3682_cov_14.947615, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000134 DAONQZ010000000
VERSION     DAONQZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_3682_cov_14.947615"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1816)
                     /gene="groL"
                     /locus_tag="PLR13_11815"
     CDS             complement(173..1816)
                     /gene="groL"
                     /locus_tag="PLR13_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQC20070.1"
                     /translation="MGAKILLYDEEARKSVLKGVNTLADAVKVTLGPKGRNVIIDKSY
                     GSPTVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATILAQAIYRE
                     GVKAVAAGSNPMDIKRGIDKAVETVVKELSKMSKPTKDQKEIAQVGTISANNDETIGN
                     IIAGAMDKVGKEGVITVEEAKGLETELEIVEGMQFDRGYLSPYFVTNADKMLVALEDV
                     LILIYEKKISGMKDLLPILEQIAKMGRPLLIIAEDVEGEALATLVVNKIRGTLHVAAV
                     KAPGFGDRRKAMLEDIAILTGGKMISEDMGYKLENVKLEDLGRAKKITIDKDNTTIID
                     GAGKRAELEGRVKQIRAQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATESEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVAYLRTLPALSKMDLTGDEQIGVNIVKKAIEEPI
                     KMIACNAGLEGSIVVEKVKEKKGAFGFNARTDEYEDMISAGVIDPTKVTRFALQNAAS
                     VSGLLLTTQCMIADKPEEKGAGGGMPGMPPGGGYPGMGM"
     gene            complement(1843..2133)
                     /gene="groES"
                     /locus_tag="PLR13_11820"
     CDS             complement(1843..2133)
                     /gene="groES"
                     /locus_tag="PLR13_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQC20071.1"
                     /translation="MKIRPLQDRIIVKRLEEEQKTKGGIIIPDTAKEKPIEGKVIAVG
                     KGKVADDGKLIKPEVSVGDKVLFSKYGGTEVKIDGEEYLIMREDDILGIIEK"
     gene            complement(2243..3601)
                     /gene="radA"
                     /locus_tag="PLR13_11825"
     CDS             complement(2243..3601)
                     /gene="radA"
                     /locus_tag="PLR13_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQC20072.1"
                     /translation="MKKSKTGFFCQHCGYMSPKWLGKCPSCNGWNCFAEELIVDSGSD
                     SRAEMRFDGKPLPIEDIPLTEGKRTVTGIAEVDRVLGGGIVGGSAILIGGEPGIGKST
                     LMLQVMHNLADKGLKVLYVSGEESASQIKLRSDRIGAAAKNLFVLVEVSLEKILEQIK
                     VIAPAIVVIDSIQTVYASELMSAPGSVGQVRETSSRLILFSKKNNIPVFLVGHVTKDG
                     SIAGPKVLEHMVDTVLYFEGDSSHSYRIVRGMKNRFGPTNEIGVFEMREKGLIEVPNP
                     STFFLAERPQNVAGSVVVPSLEGTRPILVEIQALVSSTSFGTPRRTAIGVDYNRVSLL
                     VAVLDKICGLHLGGSDIFINVAGGVKVEEPAVDLGIVAAMASSFLDKPIDGRTIVLGE
                     VGLAGEVRAVSQMEIRIKEAARLGFTRCLIPKTNAAQLEKTAKIDICKIGSLKEMLDY
                     LF"
BASE COUNT          800 a         1021 c          864 g          997 t
ORIGIN      
        1 aaaaaagccc cccgtccgat tggacagggg gctttaactt tatatattag agtcgcgtcg
       61 cattcgcccg gataccgagg cggcgaggag gaggctccgc aggcgtatta ctaatacgtt
      121 gaggaagccg acgacgctgc caacaaaggt agccgtgtga atgccgtcgt gactacattc
      181 ccattccggg atatccgcca ccgggaggca taccgggcat tccaccgccg gcgcccttct
      241 cttcaggctt gtcggcgatc atgcactgcg tggtcaaaag cagtccggaa acgctcgccg
      301 cgttctgcag ggcaaaacgg gtaaccttgg tgggatcaat gacaccggcc gaaatcatat
      361 cttcatattc atcggtgcgg gcattgaaac cgaaggcgcc ctttttctct ttgacttttt
      421 cgaccacaat gctgccttcg aggcccgcat tgcaggcgat catcttgatg ggttcttcaa
      481 tggctttttt cacgatattg acgccgatct gttcgtcgcc ggtaagatcc atcttggaca
      541 gtgcgggcag ggtgcgcaga taagccacgc cgccaccggc aacgattcct tcttccacgg
      601 ctgcacgggt cgcatgcaga gcatcttcca cgcgggcttt cttttccttc atttcggact
      661 cggttgcggc gccgactttg ataacggcca caccgccgac cagtttggcc agtctttcct
      721 gaagtttttc tctgtcgtaa tcggacgtgg tttcttcgat ctgagcgcgg atctgtttca
      781 cgcggccttc gagttcggcg cgtttgccgg cgccgtcaat aatcgtggtg ttgtccttgt
      841 caatggtgat cttcttggcg cgtcccaaat cttccagttt cacattttcc agtttgtagc
      901 ccatgtcttc ggagatcatc ttgccgccgg tcaggatggc gatgtcttcc agcatggcct
      961 tgcggcgatc gccgaaaccg ggggccttga cggccgctac atgcagggtg ccgcgaatct
     1021 tgttgaccac cagcgtggcg agcgcttcgc cctcaacgtc ctcggcaatg atgagcagcg
     1081 gacggcccat cttggcaatc tgttccaaaa tcggcagcag gtctttcatg ccgctgattt
     1141 tcttttcgta aatgagaatt aaaacatctt ccagagcgac cagcatttta tcggcgttgg
     1201 tcacaaagta aggagacagg taaccgcgat cgaactgcat gccttcaacg atctccagtt
     1261 cggtttctag gcctttggct tcttcaacgg taatgacgcc ttcctttcca actttatcca
     1321 tggcgccggc gatgatgttg ccgatggttt catcgttgtt ggcggaaatg gtgccgacct
     1381 gggcgatttc tttctgatct ttggtgggtt tgctcatctt gctgagctct tttacgacgg
     1441 tctcaacagc cttatcgata ccgcgtttga tgtccatggg attggaacct gcggcaacag
     1501 ccttgacgcc ttcacggtaa atggcctgag ccagaatggt agcggtggta gttccgtcgc
     1561 cggcgacgtc gctggttttg ctggcgactt ccttgaccat ctgtgcgccc atgttttcaa
     1621 atttatcttc gagttccact tctttagcta ccgtgacgcc gtccttggtc accgtcgggg
     1681 aaccgtaact tttatcgata ataacatttc ggcctttcgg gcccagtgta actttaacag
     1741 catcggccag tgtattgacg cccttcaaaa ccgattttct tgcttcttca tcgtaaagaa
     1801 gtatttttgc tcccattgta tatattcctc cttagtagtt tcttattttt cgataatgcc
     1861 gagaatgtcg tcttctctca tgatcaggta ttcttcgccg tcgatcttga cttccgttcc
     1921 gccgtatttg ctgaacagga ctttatcgcc cacgctgact tcgggcttga tgagtttacc
     1981 gtcatcggct actttgccct tgcccacggc aatgacttta ccttcaatcg gtttttcctt
     2041 agctgtatcg ggaatgatga ttcccccttt ggtcttctgc tcttcttcca aacgttttac
     2101 aatgattcta tcttgtaaag gtctgatttt cataaacttt actctccttt cctgattttt
     2161 aaaatttaag aatgctgttt taacagacgt attttatgca ccatttttga gatgtcaagg
     2221 gcacggaggg gatttatatt aattaaaata aatagtccag catttctttc agggaaccga
     2281 ttttacaaat atcgattttg gctgtttttt ccagttgagc cgcgttggtt ttcggaatca
     2341 gacaccgcgt gaagcccaga cgcgccgctt cctttatcct tatctccatc tgagagacgg
     2401 cacggacttc gccggccagc cccacttctc ccaaaacgat cgtccggcca tcaatgggct
     2461 tgtccagaaa actggatgcc atggcggcaa ctattccaag atcaaccgcc ggctcttcga
     2521 cttttactcc gccggcaaca ttgataaaaa tatcagagcc gcccagatgc aggccgcaaa
     2581 ttttgtccag gaccgccacc aaaagcgata cccggttata atccacaccg atggctgtcc
     2641 gacgcggcgt cccgaaactt gtcgagctca ccagcgcctg aatctccacc agaatcggcc
     2701 tggttccttc cagagagggc acgaccaccg aaccggcaac attttgcggc cgctccgcca
     2761 gaaaaaaagt agagggattg ggcacttcga ttaacccctt ctcccgcatt tcaaaaacgc
     2821 cgatttcgtt ggtcgggccg aaacggtttt tcattccccg gacaatgcgg taagaatgag
     2881 aagaatcgcc ctcaaaataa agcacggtgt ccaccatgtg ttccagcact ttgggaccgg
     2941 cgatcgagcc atccttggtt acatgaccga ccagaaaaac aggaatatta tttttcttgg
     3001 aaaagagaat taatcgcgag gacgtttcac gcacctgccc gacacttccc ggagcggaca
     3061 tcagttccga tgcataaacc gtctggatgg aatcaataac tacaatggcc ggagcaatga
     3121 ctttgatctg ctccagaatt ttctccagtg aaacctcaac cagaacaaat aaattcttcg
     3181 cggccgctcc gatccggtca ctgcgcaact ttatctgact ggcggactct tcgccggaaa
     3241 catagagcac ttttaatcct ttatctgcca gattatgcat gacctgcagc atgagcgtgg
     3301 atttgccgat acccggttct ccgccgatga gaatggccga tccaccgacg ataccgccgc
     3361 ccagcacccg gtcaacttcc gcgatgccgg taacggttcg cttgccttcc gtgagcggaa
     3421 tatcctcgat gggcagcggc ttgccgtcaa accgcatctc cgcccggctg tcggaaccgc
     3481 tatcaacgat cagttcctcg gcaaaacaat tccacccatt gcaggaggga cactttccca
     3541 gccacttggg cgacatgtaa ccgcaatgct gacaaaagaa acctgtcttg ctctttttca
     3601 aatctgattg ctccttaaaa attatcaaaa caaaaccata ttttcctttg ccatgtcaac
     3661 atgaattagg ccttgttatt tt
//
LOCUS       DAONQZ010000135         3596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_26372_length_3596_cov_13.314600, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000135 DAONQZ010000000
VERSION     DAONQZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26372_length_3596_cov_13.314600"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..2980
                     /locus_tag="PLR13_11830"
     CDS             131..2980
                     /locus_tag="PLR13_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQC20073.1"
                     /translation="MEFKAGALVKVRNRDWVVLPSNDDNLVLLKPLGGSEDEITGVYL
                     PLRFENDQLASTSFVWPDAGDLGDISSARLLFNAVRLSFRNGAGPFRSLAKLSFRPRS
                     YQIVPMIMALRQQSPIRLLIADDVGVGKTIEALLIIKELLERREINRFAIIALPHLCE
                     QWQGELKDKFGIDAVIIRSNTQARLDREIQGDRSVYDYYPYQIISLDYIKSDQRRQVF
                     IQEAPELVIVDEVHTCAKPTGANVAQQQRHHLVYDLAQKKNQHLILLTATPHSGKAEQ
                     FSSLLGLLKEEYSLLDLPQATQNQRKELAEHYVQRRRADVLKWMDEDTPFPKRDPGEY
                     QYDLSPAYSAFYENVLNFALGLIKGTEQKAGRARYRYWSVLALLRGVMSSPATGLEMI
                     TNKITGKETDLPDEADVLPDAETNPIMDEDYGAAKDFTPTTVFEKTDWSTSENKQLAN
                     LAAQIHNLHGLEYDKKAVRAQEIVSSWIKEGFNVVIYCRFIATANYLGEVLKPSLQKI
                     HSDINIQVVTSEDPDEVRKSRIDDMESSKHRVLIATDCLSEGINLQDKFTAVLHYDLP
                     WNPNRLEQREGRIDRFGQTAPIVKAYLLYGNNNPIDGVVLKVLLRKVREIRKATGISI
                     PFPEDSQSLIDSVLQAVLLSAPAQTQSQQLTLQFNNADEVQEKELIATKAIEAAAARE
                     TASRNIFAQHAIKAHEIEQDLQQSDEAIGTPQAVESFVTDALNHLLGAQITKDKKGYV
                     LFTANLPPVLKDTLPQTAQLKVSFYSPTPEGYLYIGRNHLFVEQLCQHLMAHSLKQDK
                     HYGPARASVIRCRYVAIKTTLLLFRVRNVIEEKQTGKQLVAEEMLTWGYQGNAGDNQI
                     LSADEIKHLMENAVPTANLTEQAKEEFLTNELGRIKNLGAALDQIAIKRSEILVEAHE
                     RFRKVIGGKRYKVVEPVLPMDLMGIYILLPDTQKQ"
     gene            2984..>3596
                     /locus_tag="PLR13_11835"
     CDS             2984..>3596
                     /locus_tag="PLR13_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20074.1"
                     /translation="MKFNTIRIEGSILSSDILDKIEQGELGGQRASDFGFDPSVKVKD
                     EIVKTWADAQDLWRIFQRHRADVAKGATGTSETRKFWMLPLLGLLGYESELSKAESVN
                     DKTYAISHRAKNLDGYPVHIMGFNDSLDKKREDSGPRMSPHALVQEYLNLTEHLYAIV
                     TNGTQLRLLRDSSRLIKLSFIEFDLEAMMEEEQYADFAVLYRLL"
BASE COUNT          991 a          886 c          873 g          846 t
ORIGIN      
        1 cagaaagatg aacaaattcg tcaggctata cgcaataagg gcgagcaggt cattgtttac
       61 tattatcgtg acagtcttga agttcttgta aacaaaaggt gcgatatatt tattaaggtg
      121 aaatagttta atggaattta aagcgggtgc actggtcaaa gtcagaaata gagactgggt
      181 tgttttgcca tccaatgacg acaaccttgt gcttttaaaa ccgctgggcg gttcagaaga
      241 tgaaataaca ggtgtttacc ttccactgcg atttgaaaat gaccaactgg catccacctc
      301 atttgtctgg ccggacgccg gtgacctagg tgatatttca tcggcccggc ttcttttcaa
      361 tgcggtgcgt ctttccttca gaaatggcgc aggccctttc cgcagtttgg ccaagctttc
      421 attccggccc cgttcatacc agatcgtacc aatgattatg gcgctcaggc aacaaagccc
      481 cattcggctt ctcattgccg atgatgtcgg cgtcggcaaa accattgaag ccctgcttat
      541 cattaaagag cttttggaac gcagagaaat aaatcgtttc gccattatcg cgctgccgca
      601 cctgtgtgaa cagtggcagg gggaattaaa agacaaattc ggcattgatg ccgtgattat
      661 ccgttccaat acacaggcgc gtcttgaccg ggaaattcag ggcgaccgca gcgtttatga
      721 ctattacccg tatcaaatta tttccttaga ctatatcaaa tcggatcagc gtcgtcaggt
      781 cttcatccag gaagcccctg aactggtaat tgttgacgaa gtgcacacct gtgctaaacc
      841 gaccggtgca aatgtcgcgc aacagcagcg acatcatctt gtttatgatt tggcacaaaa
      901 gaaaaatcag catttaatcc tgcttacggc aacgccgcac agtggcaagg cggagcagtt
      961 cagttctcta ttggggttgt tgaaagagga atacagcctg cttgatctgc cgcaggcgac
     1021 gcagaaccag cgtaaggaat tggcggaaca ttatgttcag aggcgtcggg ccgatgtttt
     1081 aaagtggatg gatgaagaca caccctttcc taaacgtgat ccaggagaat atcagtatga
     1141 tctatctcct gcctattccg ccttttatga aaacgtgctt aattttgctc tgggcttgat
     1201 caaaggcacg gaacagaagg ctggcagggc aagataccgc tactggtcgg tgctggctct
     1261 tttacgcggc gtgatgtcca gcccggcaac aggtctggag atgatcacta acaaaataac
     1321 tggaaaagaa accgatcttc cggatgaggc ggacgtctta cccgatgccg aaacaaatcc
     1381 cattatggac gaagactacg gcgcggcaaa agactttaca cccaccactg tttttgaaaa
     1441 aacggattgg tcaacctcgg agaacaagca actggctaat ctggcagccc agattcataa
     1501 tttgcacggt ctggaatacg acaaaaaagc tgtgcgcgcc caagaaatcg taagctcgtg
     1561 gataaaagaa ggattcaatg tggtaattta ttgccggttt atcgccacgg ccaattacct
     1621 tggcgaagtg ctcaaaccga gccttcagaa aatccattcg gacatcaata tccaggttgt
     1681 gaccagtgaa gatccggatg aagtgcggaa aagccgcatt gacgacatgg aatcatcgaa
     1741 acatcgggta ttgattgcca cggattgctt gagcgaaggc atcaacctcc aggataaatt
     1801 tacagccgtg cttcattacg atttaccctg gaatcccaac cggctcgaac agcgcgaagg
     1861 ccgcattgac cgcttcggac agactgcgcc gattgtaaag gcctatttgc tttatggaaa
     1921 caacaacccc atcgacggcg ttgttttaaa agtgcttttg cgcaaagtga gggaaatccg
     1981 taaggcaacc ggtatatcca tcccctttcc ggaagacagc cagtcgttaa tagattcagt
     2041 gcttcaggcg gtgcttttga gcgcgccggc acagacccag agccagcagc ttacattaca
     2101 attcaataat gcggatgaag ttcaggaaaa agaactgatt gccaccaagg ccattgaggc
     2161 tgcggcagcc cgtgaaacgg cgtcccgcaa cattttcgcg cagcacgcca tcaaggcgca
     2221 cgagatcgaa caggatcttc agcaatctga tgaagccatc ggaacccctc aggcagttga
     2281 atcattcgtg acagacgcat tgaatcatct gcttggtgct caaatcacaa aagacaaaaa
     2341 aggatatgtc cttttcaccg caaatctgcc gccggtttta aaagacacgc tcccgcagac
     2401 agcccagctt aaggtaagct tttattcgcc aacaccagaa ggctaccttt atatcgggag
     2461 aaatcatctc tttgtggagc aactctgcca gcatctgatg gcccattctt taaaacagga
     2521 caaacactat ggtccggcac gtgcctcggt gatccgctgc cggtatgttg caatcaagac
     2581 caccttgctt cttttccgtg tgcgcaatgt cattgaggaa aagcagacgg gtaaacaact
     2641 ggttgccgaa gaaatgctga cctggggcta tcagggcaat gccggagata atcaaatcct
     2701 ctcggctgat gaaataaagc atctcatgga aaatgctgtg cccacagcca atctgaccga
     2761 acaggccaaa gaagagtttc tgaccaatga actggggaga ataaagaacc tcggcgccgc
     2821 attggatcaa attgccatta agcgctcaga aatcctcgtt gaggcgcacg aaaggtttag
     2881 gaaagtcatc ggcggcaagc gttataaagt agttgaacct gttttaccga tggatttaat
     2941 gggcatttat attttacttc cggatacaca gaaacaataa tttatgaaat tcaacaccat
     3001 ccgcatagaa gggtccatcc tttcatccga catcctcgac aagatcgaac agggggaact
     3061 cggcggccaa cgagcctctg atttcggttt tgatccgtcc gtcaaggtca aggacgaaat
     3121 tgtcaagacc tgggccgatg ctcaggattt atggcggata tttcagcgtc accgcgccga
     3181 tgtggcgaaa ggcgccaccg gcacgagtga aaccagaaag ttttggatgc tgccactttt
     3241 gggccttctg ggttatgaat cggagctctc caaagccgaa agcgttaacg ataagactta
     3301 cgccatcagc caccgggcta aaaatctgga tggctatccc gtccacatca tgggttttaa
     3361 cgacagcctg gataaaaagc gcgaggactc cggcccccgc atgtcgccgc acgcccttgt
     3421 gcaggaatac ctgaatctca ccgaacatct ttatgccatt gtcaccaacg gcacccagct
     3481 tcgcctgctt cgagactcaa gccgactcat caagctctcc ttcattgagt ttgatctgga
     3541 agccatgatg gaagaagagc agtacgccga ttttgccgtt ttgtaccggc tgctcc
//
LOCUS       DAONQZ010000136         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_27916_length_3442_cov_13.576912, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000136 DAONQZ010000000
VERSION     DAONQZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27916_length_3442_cov_13.576912"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2760)
                     /locus_tag="PLR13_11840"
     CDS             complement(<1..2760)
                     /locus_tag="PLR13_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQC20075.1"
                     /translation="MLTQYEKLKDILREMFQMDQADLDFGIYRIMNAKRDEIEKFLDD
                     ELLPQVKSEFAKYRGTEIEEKKKELSQLEATLRDSGMNPDDSPKVKGIREELAKYGDD
                     EALTNEVFSHLTSFFRRYYDGGDFLSLRRYKKDTYAIPYEGEEVKLHWANADQYYIKT
                     SEYLKDYAFKLPGGKKAHFKLVEATQEKDNIKTAAGKERKFRIHEDEPIQIDGQEVFI
                     RFVYLPEKEKQDALNKKTVEFINGEATKRLKDFPELFAAAPTAKNANRTLWEKHLTSY
                     TARFNFDFFIHKDLGGFLRRELDFYIKNEVLYVDDLDGQDERQLKSAVAKIKVIKHIA
                     LKIIAFLAQLEDFQKKLWLKKKFVVNCGYCVTLDRVPEELYPEIAQNESQREEWVSLF
                     AINEIEADLSKPGYTKPLTIEFLKANPYLVLDTKHFDAKFKEKLLAIFDNLDANLNGL
                     LIQGDNFHALNLLQALYREQINCTYIDPPYNTDASAIIYKNNFKDSSWLSLVHNRIEL
                     VHGLMRKDSIICVAIDDQEVASLRQVMDGIFEKVIGVSVVKSNPQSRKTTGKFSPVHE
                     YAIFYGKTDEAMPCSIGSNGSKAARYPLIDEKGRFSWMNFIRAGNNDRREDRPKLFYP
                     IAVNKDKRIRIPKMTWIEELQEYKLLEEIDADEKLIYPIKYSDGKAIEKNWQRGHKRV
                     SEEYEEYRVRRDNNGDINIDFKTRMDEEAAPVTWWDKGEYASANYGAAELKDLFGEKN
                     FDFPKAKKLVEDALRACGASNYNSIVIDFFGGSGTTGHATINLNREDGGKRKYILIEM
                     GEHFDTVLKPRIQKVIYSKDWKDGKPVSRDGISHMFKYMYLESYDDTLDNLKAIRTKD
                     QETLLEKMSAGAHEDYTLSYMLNVETKASDSLLNIDRFIDPFNYTLKIRRDNELKDEK
                     IDLVET"
     gene            complement(2784..>3442)
                     /locus_tag="PLR13_11845"
     CDS             complement(2784..>3442)
                     /locus_tag="PLR13_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20076.1"
                     /translation="REIKTPVAELIFDISASPLRISVIEQLKGKSGWLILTKLTIKSF
                     ENDEYLLFNAFDDEGMVIHPEVCEKLFDCTARLNEITLPETEKEKLMTDARIHSDATL
                     NDVMERNNSFYQEECERLFRWADDLMLSAEKELKDTKNQLRELNRQLRLAINQQERLD
                     VELKVKSLGKKQREQRQKIFDVEDEIASKRDVLIDQLKQRLQQKTQAEHLFTIRWNVV
                     "
BASE COUNT          851 a          806 c          680 g         1105 t
ORIGIN      
        1 cgtctccacc agatcgattt tttcgtcttt gagttcgttg tcccggcgga ttttaagcgt
       61 gtagttaaaa ggatcgataa agcggtcgat attcaaaagc gagtcgctgg ccttggtttc
      121 gacattcagc atgtaggaaa gcgtataatc ttcgtgggcg cccgcgctca ttttctccag
      181 cagtgtctcc tggtcttttg tgcgaatggc tttcaaatta tccagcgtgt cgtcatagga
      241 ttccagatac atgtatttaa acatgtggct gatgccatcg cgcgacacag gttttccgtc
      301 tttccaatct ttggaataaa taaccttctg gatgcggggt ttcaggacag tgtcgaaatg
      361 ctcgcccatt tcgataagga tatacttacg cttgccaccg tcttcacggt tgaggttgat
      421 ggttgcatgg ccagtcgttc cagaacctcc aaaaaaatct atcactatgg agttataatt
      481 tgacgcgcca caagcacgca aggcatcttc aaccaatttt tttgccttcg gaaagtcaaa
      541 atttttctca ccaaaaaggt cctttaactc ggcggcgcca tagttagcag atgcatattc
      601 acctttatcc caccacgtta ctggcgcagc ttcttcatcc atccttgtct taaaatcaat
      661 attaatatcg ccattgttat ctctccgaac gcgatattct tcgtattctt cagagaccct
      721 tttatgccct ctttgccaat tcttctctat ggcttttcca tcagagtatt taataggata
      781 tattaacttt tcatcggcgt cgatttcctc aagcaacttg tattcttgta attcttctat
      841 ccatgtcatt ttgggaatac gaattctttt atccttattt accgcaattg gataaaataa
      901 ttttggtcta tcttcgcgac gatcattgtt tccggcacga ataaaattca tccaagaaaa
      961 tcttcctttt tcatcaatca acggatatct tgcagcttta gagccattag aaccaattga
     1021 gcaaggcatt gcttcgtcgg ttttcccgta aaaaatagca tactcatgaa cgggtgaaaa
     1081 tttacccgtt gtctttcggc tttgcggatt agacttaaca actgaaacac caattacctt
     1141 ctcaaaaata ccatccataa cttgtctaag agacgcaact tcttgatcat caatcgcgac
     1201 acagataata gaatcttttc tcataagacc atgaacaagc tcaatcctat tatggactaa
     1261 acttagccaa gaagaatctt taaaattgtt tttatagata attgcggacg catcagtgtt
     1321 gtatggagga tcaatataag tgcaattaat ttgctcgcga tacaatgcct gcaataaatt
     1381 caaagcgtgg aaattatcgc cttgtatcaa taaaccattg agatttgcat ccaggttatc
     1441 aaagattgcc aacagttttt ctttaaactt cgcgtcaaag tgtttcgtgt ccaaaaccag
     1501 atagggattg gctttaagaa attctatcgt cagcggcttt gtgtagccgg gcttggataa
     1561 atccgcctca atctcattaa tggcaaagag actcacccat tcctcgcgct ggctttcgtt
     1621 ttgggcaatc tccgggtaga gttcttccgg cacgcggtcg agtgttacgc agtaaccgca
     1681 attcaccaca aatttcttct tcagccagag cttcttttga aaatcttcca gttgcgccag
     1741 aaaggcaata atcttcaggg caatgtgttt gataaccttg atcttggcca ctgcgctttt
     1801 gagctgcctt tcgtcctgcc catccagatc atccacataa agcacttcat tctttatgta
     1861 aaaatcaagt tcgcggcgta aaaatccgcc caaatcctta tgaataaaaa aatcaaagtt
     1921 gaaccttgca gtataagatg tcaggtgttt ttcccatagt gtgcgattgg catttttggc
     1981 ggtcggcgcg gcggcaaaga gttccggaaa atcttttagt ctctttgtag cttcgccgtt
     2041 gatgaattca acggtttttt tgttgagcgc gtcctgtttt tctttttccg gcaaatagac
     2101 aaaccggata aagacttcct gcccgtcaat ttgaatcggt tcatcttcat gaattcgaaa
     2161 tttacgttcc ttgccggcgg cggtcttaat attatccttt tcctgcgtgg cctccaccag
     2221 tttgaaatga gccttctttc cgccgggaag tttgaaggca taatccttca ggtattcgct
     2281 tgttttaata taatattgat cagcattggc ccaatggagc ttgacttcct cgccttcata
     2341 gggaatggca taggtgtctt ttttataccg gcgcagcgaa agaaaatcac ctccgtcata
     2401 ataacgccgg aaaaaagatg tcaggtgcga aaagacttca ttggtcagcg cctcatcgtc
     2461 gccgtatttg gccagttctt cacggatgcc ctttactttg ggcgaatcat cgggattcat
     2521 gccgctgtcc cgcagcgttg cttctaactg agaaagctcc tttttctttt cctcaatttc
     2581 cgttccacga tacttggcaa attctgactt cacctgggga agaagctcat catccagaaa
     2641 tttttcaatc tcgtcgcgct tggcgttcat aatgcggtag atgccgaaat ccagatcggc
     2701 ctggtccatc tgaaacatct cccgcaggat atctttcaac ttctcatact gtgtcagcat
     2761 ggttccttca ctcctttttt cgcttaaacg acattccagc gtatcgtaaa taaatgttct
     2821 gcctgagtct tttgttggag acgctgcttg agctggtcaa tcagaacgtc ccgttttgat
     2881 gcaatttcgt cttccacatc aaatattttc tggcgctgtt cgcgctgttt cttgcctaag
     2941 cttttaacct tcaattccac atccaggcgc tcctgttggt tgatggctag gcgaagctgg
     3001 cggtttagct cccggagctg gttcttggtg tctttgagtt ccttttccgc tgatagcatc
     3061 aggtcatcgg cccagcggaa aagcctttcg cattcttctt gataaaagct attattccgt
     3121 tccatgacat cgtttaatgt cgcatctgaa tggatgcggg catcagtcat taatttttct
     3181 ttctctgttt ccggcagggt tatctcgttt agtctcgccg tacaatcaaa taacttttcg
     3241 catacttcgg gatggattac catgccttca tcatcaaaag cgttaaaaag cagatattca
     3301 tcattttcaa aacttttaat ggtgagtttt gtcagtatca gccacccgct ttttcccttg
     3361 agttgctcaa tcacggatat acgaagaggg cttgctgaaa tatcaaatat taattcggcc
     3421 accggtgttt taatttcgcg cg
//
LOCUS       DAONQZ010000137         3322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_29221_length_3322_cov_13.520967, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000137 DAONQZ010000000
VERSION     DAONQZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29221_length_3322_cov_13.520967"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLR13_11850"
     CDS             <1..368
                     /locus_tag="PLR13_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQC20077.1"
                     /translation="KDFLGKTDYDEKQNRRFFKNPYVAGYVIALSCPITIVWWTGVFG
                     ALLASQDSIQTNFSAYLSCLTILLGCFIWIVFLSAALHWGRKIVNEKFIGFVSLIAGM
                     CLAGFGLAFLCRSVHLIIH"
     gene            complement(403..1842)
                     /gene="gltA"
                     /locus_tag="PLR13_11855"
     CDS             complement(403..1842)
                     /gene="gltA"
                     /locus_tag="PLR13_11855"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HQC20078.1"
                     /translation="MKNISVGAMETKETKPKKEKVPRQTMPEQDPKERIHNFEEVPFG
                     YSEETAIIEAQRCIQCKKPGCVSGCPVDVKIPEFIKAIAEGRFIDAALKLKETNGLPA
                     VCGRVCPQEDQCEKECILGKKGEPVAIGRLERFAADYERNSGKISIPPAVQSNGKKIA
                     VAGAGPAGLTIAGDLVKLGYDVTIFEALHKAGGVLVYGIPEFRLPKAIVEAEVDYLKK
                     MGVKIETNFVIGRTKTIDELFADGFDAVFIGVGAGAPVFMNIPGENLSGIYSANEYLT
                     RSNLMKAYLFPEYDTPIVRGKKVAVVGGGNVAMDSVRTALRLGAERAMIIYRRTEVEM
                     PARKEEVHHAQEEGVEFLLLHNPVEYIGNEAGWVTGMKCIKMELGEPDASGRRSPVPI
                     QGSEFVIDVDTVVVAVGTMANPIVPATTTGLETNKKGYILTKDESGLTSREGIYAGGD
                     IVTGSATVILAMGAGRKAANAIHEYLSNK"
     gene            complement(1808..>2011)
                     /locus_tag="PLR13_11860"
     CDS             complement(1808..>2011)
                     /locus_tag="PLR13_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HQC20079.1"
                     /translation="NSIMVDATGMCGACRVDVGGKTRFTCVDGPEFDGHQVDFKLLAD
                     RLRMYCDQEREAYEKHKCGCDGN"
     assembly_gap    2012..2111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2112..2335)
                     /locus_tag="PLR13_11865"
     CDS             complement(<2112..2335)
                     /locus_tag="PLR13_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HQC20080.1"
                     /translation="MNKIIEKENLSESVVRMVLEAPVVAQKRKAGQFIVLKIHEKGER
                     IPLTIVDSDAQKGTITIIFQVVGKTTALLAA"
     gene            complement(2487..>3322)
                     /locus_tag="PLR13_11870"
     CDS             complement(2487..>3322)
                     /locus_tag="PLR13_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HQC20081.1"
                     /translation="NPYIPYPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIPGQ
                     FGELSIFGKGESPIGIASSPTQKGYVEFTVQKAGVVTSALHEMEEGTVMGIRGPLGNS
                     WPLDFLAGKNIVIVGGGFAFTTLRSLINYMLFEDNRKRFGNITVIYGARNPGLLLYKD
                     ELVEWGKRADINLNVTVDKGDATWKGKEGFVPAVCKEVAPSKENAVAVICGPPVMIRF
                     TLPVFFDLGFSKQNILTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLTPE
                     Y"
BASE COUNT          737 a          880 c          745 g          860 t
ORIGIN      
        1 taaaagattt cctgggcaaa acggactatg atgaaaaaca gaatcggcgg ttttttaaaa
       61 atccctacgt cgccggttac gtgattgcgc tttcttgtcc gatcaccatc gtctggtgga
      121 cgggtgtttt cggcgccctg ctggcatctc aggacagcat acaaacaaat ttttccgcat
      181 atctttcgtg tctcactata ttgctcggct gcttcatctg gatcgttttt ctctccgccg
      241 cgctgcactg ggggagaaaa atcgtcaatg aaaaatttat cggatttgtt tcgttgatcg
      301 ccggcatgtg tctggcggga ttcggcttgg cttttctatg ccggtccgtg catctgatca
      361 tacattgaca gcccgggatc aggcccgggc tgccggattg aactatttat tggaaagata
      421 ctcgtgaatc gcgttggccg ccttgcgccc tgcgcccatc gcgagaataa ccgttgcgga
      481 accggtgaca atatcgcctc cggcataaat gccttcgcgg ctggtcagcc ccgattcatc
      541 cttggttagt atatatcctt tcttattcgt ttccaaaccg gtggtggttg cggggacaat
      601 cggattggcc atggtgccga cggcaacgac aacggtatcc acatcaatca cgaattcgga
      661 tccttgaatg ggaacggggc ttcgtcttcc ggacgcatcc ggctcgccaa gttccatttt
      721 gatgcacttc atcccagtga cccagccggc ttcgttgccg atatattcca ccggattgtg
      781 cagcaggagg aattcaacgc cctcctcctg cgcgtgatgg acttcttctt tcctggcggg
      841 catttccact tcggtgcggc ggtaaataat catcgccctt tccgcgccga gtctcaaagc
      901 cgtacgcacg gaatccatcg cgacgttgcc gccgccgacc actgcgactt tcttaccgcg
      961 aacgatcgga gtgtcgtatt cgggaaacaa ataggccttc atcagattgg agcgcgtcag
     1021 atattcattc gccgaataga tgccgctcag attctcgccg gggatgttca taaagaccgg
     1081 agcgcctgcg cccaccccga tgaaaacggc atcaaatcca tcggcaaaaa gttcatcgat
     1141 tgttttcgtg cggccgatga caaaattcgt ttctatctta acgcccattt ttttgagata
     1201 gtccacttcc gcttccacaa tggccttcgg caaacgaaac tccggaatgc cgtaaaccag
     1261 cacgccgccg gccttatgca gagcttcaaa gatggtgaca tcgtaaccca gtttaaccag
     1321 atcgcccgca atggttaatc ccgctggacc ggcgccggca acggcaattt ttttgccgtt
     1381 tgattgcacg gccggaggaa tggaaatttt gccgctgttg cgctcatagt ccgccgcgaa
     1441 acgctccaac cgcccgatgg ccacgggctc gcctttcttg ccgagaatac attccttttc
     1501 gcactgatct tcctgcgggc agacgcggcc gcagacagcg ggcagaccgt tggtttcttt
     1561 taatttcaag gcggcgtcaa tgaaacgacc ttccgcgatg gccttgataa actcgggaat
     1621 cttgacatcc acgggacagc cgctgacgca gccgggtttc ttacactgga tgcagcgctg
     1681 cgcctcgata atcgccgttt cttcggaata gccgaaaggc acttcttcaa agttgtgaat
     1741 cctctctttg gggtcctgtt ccggcatggt ctgccggggc actttttctt tctttggctt
     1801 cgtttcttta gtttccatcg cacccacact tatgtttttc ataggcttcc cgttcctgat
     1861 cacaatacat cctcaggcgg tcggccagta atttaaaatc aacctggtga ccatcaaatt
     1921 caggaccgtc cacacaggta aacctggttt ttccacccac atcaacacgg caggctccgc
     1981 acattcccgt agcatcgacc atgatcgaat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn ngccgcaagc aacgccgttg tcttgcccac aacttgaaaa ataatggtga
     2161 tcgttccctt ctgcgcatca gaatcgacaa tggtcagagg aatacgttcc cctttttcat
     2221 gaatcttaag aacgataaac tgtccggctt tccgcttttg cgccacgacc ggagcctcca
     2281 gaaccattct gaccacgctc tcggataaat tttccttttc aataatttta ttcaagctta
     2341 atttctcctc catgaaaaaa acaggtgttc gtctttatta aattttaacg tgaaaaaata
     2401 cttaaggtaa aaaaaacagc accacctgcc aaccattcca tgattgaaca gttcttcgta
     2461 tcgcagattc tttgctgaaa cgttttttaa tattccggtg tcagtttgtc cagttccgcc
     2521 agagaaaata ccggaccgtc tttgcaaaca tatttactgc ccacgttgca gcgtccgcat
     2581 ttgccgatgc cgcatttcat tctcatttcc agggacgtga gaatgttttg cttggaaaag
     2641 cccagatcga aaaataccgg aagggtgaaa cggatcatga ccggaggccc gcagataaca
     2701 gcgacagcgt tttctttact gggcgcgacc tctttgcata ccgcagggac aaaaccttct
     2761 ttgcccttcc aagtggcgtc gcctttgtca accgtgacat tcaaattgat atccgctctt
     2821 ttaccccatt cgaccagttc atctttataa agcagcagtc cgggatttcg tgcgccgtaa
     2881 atcaccgtga tatttccgaa gcgtttgcgg ttgtcttcaa aaagcatgta attgatgagc
     2941 gaccgcagag tggtgaaggc gaaaccgccg ccgacgataa cgatattttt gcctgccaga
     3001 aaatccagcg gccaggaatt gcccaaagga ccacgaatac ccatcacggt tccctcttcc
     3061 atttcgtgaa gcgccgaggt gaccacgccg gctttctgta ccgtgaattc cacgtaccct
     3121 ttctgtgttg gagacgaggc aatgccaatc ggcgattcgc cttttccgaa aatggaaagt
     3181 tcgccgaatt gcccgggaat gtaagcatat ttctgttcgt cttccggatt cacgaattta
     3241 aaacggaatg ttttaatgtc tttggtgtcg acttcggtaa tgattttatc caccacaacg
     3301 ggatacggta tatacgggtt ct
//
LOCUS       DAONQZ010000138         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_30373_length_3224_cov_10.520669, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000138 DAONQZ010000000
VERSION     DAONQZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30373_length_3224_cov_10.520669"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLR13_11875"
     CDS             <1..520
                     /locus_tag="PLR13_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQC20082.1"
                     /translation="NHKSRNRVLPVRFGKEGKMTVAEPKQEEVPPLPPPPPPETIAPP
                     PPPPPVQEKVSISLNIEFDTAKSFIKKKYHNNIKQVADFMQTYPDTNVVIEGHTDNVD
                     RFNNPENNIKLSQARADSVRKYLIDKFGIDESRITAVGYGPSRPIADNNTPEGRMKNR
                     RVEAVIEKMVTK"
     gene            768..1349
                     /locus_tag="PLR13_11880"
     CDS             768..1349
                     /locus_tag="PLR13_11880"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQC20083.1"
                     /translation="MAAARTVFAQKGYNGARIDEIAARANVNKATIYYQIGDKDTLYT
                     GALHQVIGHVARDIADAVSRKVHPEEKMKAYITFIAGAVEANPDLPPMMMREIASGGA
                     TLPRLVIEDIASVMSILAGILEAGKEQGIFIETSPFLIHMMIMGTILFFKKGSPIKDK
                     QSWLPEALLAYDQKISANLGEEVASLVLKAIKQ"
     gene            1377..2387
                     /locus_tag="PLR13_11885"
     CDS             1377..2387
                     /locus_tag="PLR13_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQC20084.1"
                     /translation="MKKRIIFVVFIIFFLIVGALVYFGQKSNREKELVYSGTIDAIQA
                     NLSFQVAGRVSQVFVQEGQSVSQNQMIAELDRSEFESRFEQARANLERAQKTRRQLET
                     VLELSRKTLPAEVARAKAGVQSAKDSLKDAEKNYRRFEELFGKNVVSEKERDALRLQY
                     DVAQSRLAESESVLKLAQGNLTRIDAVKQDIDVAAAQINAAAASLNQAEIQLNYTQLK
                     SPANGVVTSRNIEPGETVTPGREVITVSDLSRVDLKIFVDETEIGKVKPGQKVDVQVD
                     TFPGKTYTGYVSFISPEGEFTPKVIQTTKERVKLVYLVKVSIGNPHLELKAGMPADAW
                     LK"
     gene            2400..3173
                     /locus_tag="PLR13_11890"
     CDS             2400..3173
                     /locus_tag="PLR13_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQC20085.1"
                     /translation="MTKAPESPPSMVDVRNVTMRFKSVEAVKDISLQIPKGHVLGLVG
                     SDGAGKSTLLRLVATMIQPTSGNIYIDGLHVVTDKQKIKNIIGYMPQRFGLYQDLTVE
                     ENMDFFMDIFAVPRRERKERKEKYLGFSNLLPFTDRMAGNLSGGMKQKLGLACVLVHE
                     PQLLILDEPTNGVDPVSRSEFWEILENMQKEGMTIMVSTAYLDEGEKCHRLGLMHNAM
                     LLDLAPPAVIRGGYPTLEEAIIERITQADGALAHDTFQL"
BASE COUNT          904 a          806 c          824 g          690 t
ORIGIN      
        1 caaccacaaa agcagaaaca gggtactgcc cgtgcgtttc ggcaaggaag gcaagatgac
       61 tgtagccgaa ccgaagcagg aagaagtgcc gccgctgcct ccacctccgc cgccggaaac
      121 gatagcgccg cctcctcctc ccccgccggt gcaggagaag gtctccattt ccctcaatat
      181 tgaatttgat actgcaaaat cgttcatcaa gaaaaagtat cacaataaca tcaagcaagt
      241 cgcggatttc atgcagacgt atccggatac caacgttgtt atcgaaggcc atacggataa
      301 cgtcgaccgt ttcaataatc ccgaaaacaa tataaaactc tcccaggcac gggccgacag
      361 tgtccgcaaa tatttgatcg ataaattcgg catcgatgag tcccgcatca ccgctgtggg
      421 ttacggaccc tccaggccga tcgccgacaa taatacacct gagggacgta tgaagaatcg
      481 ccgggttgaa gcggttatag aaaaaatggt tacaaaatag tttccaaagc tttcagaaat
      541 tcaacacaaa atgccccgtg attgcgcaat cacggggcat ttctcttctc acacaagaaa
      601 atctcttgac aaaaaaagga ttttttactt taatttaaat aaatagttaa aataactgtt
      661 tagtttaaaa tataccgcaa tcctttgtat tttataatat tctaatttaa cataaaaata
      721 tggaccgcaa gcgcaaagat aaatcagata aaacatcaga acgcattatg gcagcagcca
      781 gaacggtttt tgcccaaaag ggatacaacg gcgcacgcat tgatgaaatt gcagcgcgcg
      841 ccaatgtaaa caaagcaacc atctattacc agatcggcga caaggacacg ctttataccg
      901 gcgcgctcca tcaggtgatc ggtcatgtcg cccgggacat cgccgacgcc gtttcgcgca
      961 aagttcatcc ggaagaaaaa atgaaggcct atatcacttt catcgccggc gccgtggagg
     1021 cgaatcccga tctgccgccg atgatgatgc gcgaaatcgc ttccggcggc gcaacgcttc
     1081 cgcgacttgt catcgaggat attgcctcgg ttatgagcat cctggcgggc attctggagg
     1141 ccggtaaaga acaggggata ttcatcgaaa catcgccttt tctgattcac atgatgatta
     1201 tgggaacgat tctttttttt aaaaaagggt cgcccatcaa agacaaacaa tcctggctgc
     1261 cggaagcgct tctggcgtat gatcaaaaaa taagcgcgaa cctgggggag gaagtggcgt
     1321 ctttagtgct caaagcgata aaacaataac catctcaatc aaaacggagg tcggcattga
     1381 aaaaaagaat catttttgtt gtctttatca ttttctttct gattgtcgga gcgctcgttt
     1441 atttcggcca gaaaagtaac cgggagaaag agctcgtata ttccggcacg atcgacgcaa
     1501 tccaggccaa tctgtcgttt caggtagccg gccgcgtttc gcaggttttc gttcaggaag
     1561 gccagtcggt ctcgcaaaat cagatgatcg ccgaactgga ccgttcggaa ttcgaatccc
     1621 gcttcgagca ggcccgggcg aatctggaac gcgcgcaaaa aaccagaaga caactggaga
     1681 cggtgctgga actcagccgg aaaacgctcc ccgcggaagt tgcccgggct aaagccggcg
     1741 tccagagcgc aaaagattcc ttgaaggatg cggaaaaaaa ttacaggcgc tttgaagagc
     1801 tgtttggcaa gaacgtggtt tcggaaaaag aacgcgacgc cctgagactg caatatgacg
     1861 ttgcgcaatc acgcctggct gaaagcgaat ccgttctgaa gcttgcgcag gggaatctga
     1921 ccagaatcga tgcggtcaaa caggatatcg acgtggcggc cgcacagatc aacgcggctg
     1981 cggcttcctt aaatcaggcg gaaatccagt tgaactacac gcaattgaaa tcgccggcaa
     2041 acggcgtcgt caccagccgc aatatcgagc cgggggaaac ggtaacgccc ggcagggaag
     2101 tcattaccgt ttccgatctg tcccgcgttg atttgaaaat cttcgttgat gaaacggaaa
     2161 tcggcaaggt caagccgggg cagaaagtcg atgttcaggt tgacaccttc ccggggaaaa
     2221 cctacacggg ctatgtgtcg tttatttctc cggaagggga atttaccccg aaagttattc
     2281 agacgaccaa ggaaagggta aaactcgttt atctggttaa ggtatccatc ggcaatcccc
     2341 atcttgaact caaagccggc atgccggcag atgcctggct caaatagcgg agtgcgtcca
     2401 tgaccaaggc accggaaagc cccccgtcca tggtggacgt cagaaatgtg accatgcgct
     2461 ttaaaagcgt cgaggccgta aaggatattt cattgcagat tcccaaagga cacgtgctgg
     2521 gattggtcgg ctccgacggc gccggcaagt ccacgctcct gcgtcttgtc gcgaccatga
     2581 tacaaccgac cagcggcaac atttacattg acggtcttca tgtcgttacc gataaacaaa
     2641 aaataaaaaa tatcatcggc tacatgcctc agcggttcgg actgtatcag gatctgaccg
     2701 tcgaagagaa catggatttc ttcatggaca tctttgccgt gccgcggcgc gagagaaaag
     2761 agcgcaaaga aaaatatctg ggcttttcca atctgctgcc gtttacggac cggatggcgg
     2821 gaaacctttc cggggggatg aaacagaaac tgggactggc atgtgtgctg gtgcatgagc
     2881 cgcagttgct gatcctggat gaaccgacca acggggtgga tccggtatcg cgcagcgaat
     2941 tctgggagat tctggaaaac atgcagaaag aaggcatgac gatcatggtg tccaccgcct
     3001 acctcgacga aggagaaaag tgccacaggc tcggactgat gcacaacgcg atgcttctgg
     3061 atctggcgcc gcccgccgtc attcgcggcg ggtatcccac gctggaagag gcgattattg
     3121 aacgaatcac acaagcggac ggagcgctgg cacatgacac ctttcaactc tgattccatt
     3181 tccgtccgtc atctggaaaa gagattcggc aattttgttg ccgt
//
LOCUS       DAONQZ010000139         3198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_30679_length_3198_cov_18.892459, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000139 DAONQZ010000000
VERSION     DAONQZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30679_length_3198_cov_18.892459"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="cysS"
                     /locus_tag="PLR13_11895"
     CDS             complement(<1..1199)
                     /gene="cysS"
                     /locus_tag="PLR13_11895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQC20086.1"
                     /translation="MPQKIFNTATRKKEVFQPIQEGAVGIYVCGITAYDVCHIGHARA
                     AVVFDVVVRYLRAQGYNVTYVKNFTDIDDKIIDRANREGVSIADIAERYIKAHDEDMA
                     ALGVQVPTMTPRATENMQEMIALIKVLEDKGYAYCVEGDVYFSISDYKQYGGLSGRNL
                     EEMMAGARVDVSDKKKNPLDFALWKNSKEGEPFWESPWGKGRPGWHIECSAMSRRYLG
                     ETFDIHGGGEDLIFPHHENEIAQSAAATGKPLARYWMHNGFVKINSEKMSKSLGNIFP
                     VKEILKHYHPEVLRLFMLQSHYRSPVDYSDDSLSEARAALFRCYRTLQQMKELQGQLA
                     AGSKPVATQPSGRVDDYVNAFQMLKDKFNEAMDDDFNTAQALGYVFDMVRQMNNFMMK
                     EKSMPVA"
     gene            complement(1218..2255)
                     /gene="argC"
                     /locus_tag="PLR13_11900"
     CDS             complement(1218..2255)
                     /gene="argC"
                     /locus_tag="PLR13_11900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQC20087.1"
                     /translation="MIKAAIFGASGYTGQELIRILSGHPDVKLVAATSRRFAGQEVST
                     VFPALQGLTALKYQNATPSEICRLCDVVFLALPHGVSMDMAPEFINAGKTVIDLSADY
                     RLRDLKIYEVWYGKHASAKFIKNAVYGIPELYRQKIKKTKFVANPGCYPTGIILGLAP
                     ALKKQLLDVSTIIADSASGVSGAGREPFAGSLFCEVDGGFKAYKVGKHRHLPEIEQEL
                     NALAGKKFAVSFTPHLLPVKRGILSTIYAKLKKDIALGDLHALYSSFYAGETFVRMCP
                     EGVYPNISSVCGSNYCDIGLAVDPRTKRVIIMAAIDNLVKGAAGQAVQNMNIMCGFKE
                     DTGLQIAPLFP"
     gene            complement(2303..2695)
                     /gene="rpsI"
                     /locus_tag="PLR13_11905"
     CDS             complement(2303..2695)
                     /gene="rpsI"
                     /locus_tag="PLR13_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQC20088.1"
                     /translation="MAEQRFYATGKRKSAIARVYMKAGNGNIVVNKRSYEEYFPRPSL
                     KMLIKQPLDITGKKDQFDLYINVTGGGVAGQAGAVKHGISKALLEYDAELRSVLKKAG
                     FLTRDARVKERKKYGQPGARKRFQFSKR"
     gene            complement(2737..3168)
                     /gene="rplM"
                     /locus_tag="PLR13_11910"
     CDS             complement(2737..3168)
                     /gene="rplM"
                     /locus_tag="PLR13_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQC20089.1"
                     /translation="MMKTYMAKAQTADRKWYIADASGKVLGRFASEIAHRLRGKHKAT
                     YTPHVDTGDFIIVVNAEKIVLTGKKMTDKIYYSYSGYPGGLRETTAGKMLAEKPEDLI
                     RIAVQGMLPKTNLGRRMLKKLKVYSGNAHIHEAQCPKTLSI"
BASE COUNT          758 a          861 c          701 g          878 t
ORIGIN      
        1 tcggccaccg gcatgctttt ttctttcatc ataaaattat tcatctgccg gaccatatca
       61 aaaacatagc ccaatgcctg ggccgtgttg aaatcatcat ccatcgcttc attaaactta
      121 tctttcagca tctggaaggc attgacatag tcatcgacgc gcccggacgg ctgcgttgcc
      181 accggtttgg atccggcggc taattgtccc tgaagttctt tcatctgctg gagcgttctg
      241 taacatctga acagagcggc tctggcttcg gacagcgaat catccgaata atcgaccgga
      301 ctgcggtaat ggctttgcag catgaacaaa cgcaacactt ccggatgata atgtttcagg
      361 atttccttga ccggaaatat attgcccagc gatttggaca tcttttccga attgattttt
      421 acaaaaccgt tgtgcatcca gtaacgcgcc agcggcttgc cggtcgccgc cgccgattga
      481 gcgatttcat tttcatgatg cggaaaaatc aaatcctccc cgccgccgtg aatatcaaac
      541 gtttctccca gataacgccg gctcatggcc gaacattcaa tatgccagcc ggggcggcct
      601 tttccccacg ggctctccca aaagggttcg ccttccttgc tgtttttcca gagtgcaaaa
      661 tccaggggat tttttttctt gtcgctgaca tcaacacggg cgccggccat catttcttcc
      721 agattgcgac cggataaacc gccgtactgt ttataatcac tgatggaaaa atacacgtct
      781 ccctcaacgc aataggcata gcctttgtcc tccagaactt taatcagagc aatcatttcc
      841 tgcatgtttt cggttgctct gggggtcatg gtggggacct ggacacccag cgccgccata
      901 tcttcatcgt gtgccttgat gtatcgttcg gctatatccg caatactcac gccttctctg
      961 ttcgcccggt cgataatttt gtcgtcaata tccgtgaagt ttttcacata ggtgacgtta
     1021 tagccctggg cccgcaggta tctgacgacc acgtcgaaaa caaccgccgc acgggcatgc
     1081 ccgatgtgac agacgtcata ggcggtaatg ccgcacacat aaatgccgac ggcgccctcc
     1141 tgaatgggct gaaaaacttc cttttttctg gtcgctgtat tgaatatctt ctgaggcatt
     1201 tttttcacct ttacgcatca gggaaagagc ggcgcgattt gcaggccggt atcttcctta
     1261 aatccacaca tgatgttcat attctgcacg gcctgtccgg ccgccccttt caccagattg
     1321 tctatggccg ccatgataat gacccgtttt gtccgcggat caacagccaa cccaatgtcg
     1381 cagtaatttg agccgcatac ggacgaaata ttcgggtaaa cgccttcggg acacatccgg
     1441 acaaacgtct ccccggcgta aaagctgctg taaagggcat gcagatcgcc caacgcaata
     1501 tcttttttca gtttggcgta aatcgtgctg agaatgcccc gttttaccgg cagtaaatgc
     1561 ggcgtaaagg aaacggcaaa ttttttgccg gccagcgcgt tgagttcctg ctcaatttcc
     1621 ggcagatggc gatgctttcc gactttataa gctttgaagc cgccgtcaac ttcacaaaac
     1681 agcgaaccgg caaacggttc ccgtcccgcg ccgctgacgc cggaggcgga atccgcgata
     1741 atcgtggaaa catccaataa ttgctttttc agcgccggag ccagccccag aataatgccg
     1801 gtcggataac agccgggatt agccacaaat ttcgttttct ttattttctg cctgtaaagc
     1861 tccggaatgc catacaccgc atttttaata aatttggcgc tggcgtgctt gccgtaccag
     1921 acttcataaa ttttcaaatc tcttaaacgg taatcagcgc tcaaatcaat caccgtcttt
     1981 ccggcgttga taaattccgg agccatgtcc atggacacac cgtgaggcag agccagaaac
     2041 accacatcgc aaagcctgca tatttcagac ggtgttgcgt tctgatattt taacgcggtc
     2101 aaaccctgta aagccggaaa cacggtcgat acttcctgac cggcaaaccg ccttgacgtg
     2161 gctgccacta atttaacgtc aggatgtcct gacagaattc tgatcagttc ctgacccgta
     2221 taaccgctgg cgccaaatat tgctgcctta atcataaaac gctcccaaaa aaaagggagg
     2281 ctggaagccc ccctgtcgga atttaacgtt tggagaattg gaatctcttc cgggctccgg
     2341 gctgcccgta tttcttgcgt tctttaaccc gggcatctct ggttaaaaaa ccggcctttt
     2401 tcaggaccga tcgcaactcg gcatcgtact ccagaagcgc cttggaaatg ccgtgcttca
     2461 cggctcccgc ctgcccggca acgcctccgc cggtgacgtt gatatataaa tcaaactgat
     2521 ccttcttccc cgtgatatcg agaggctgct tgataagcat tttcaggctg ggacggggga
     2581 aatattcctc gtagcttctt ttattaacca caatattgcc gttgcctgct ttcatataaa
     2641 cgcgggcgat ggcacttttt ctctttcctg ttgcataaaa cctttgttcc gccatgattt
     2701 ctccaatgtt aaatcttgtt tatagttgat tgtccgttaa atcgataatg ttttcggaca
     2761 ctgtgcttcg tgaatatgag cattaccgga ataaactttc aattttttaa gcatcctgcg
     2821 tccgagattc gttttgggaa gcattccctg cacggcgata cgaatcaaat cttccggttt
     2881 ttcggcaagc attttccctg cggtcgtttc tctcaacccc ccgggatagc cggaataaga
     2941 ataataaatt ttatccgtca ttttcttgcc ggtcagaacg atcttttcgg cattcacgac
     3001 aataataaaa tcacctgtat caacgtgcgg tgtatatgtt gccttatgtt tgcctcttaa
     3061 acggtgcgct atttcactgg caaatcttcc cagtaccttg ccggaagcat ctgcaatgta
     3121 ccattttctg tcggctgtct gcgccttagc catgtacgtc ttcataataa aatcaccact
     3181 ttaatgaaaa atttaaag
//
LOCUS       DAONQZ010000140         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_31334_length_3148_cov_16.623666, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000140 DAONQZ010000000
VERSION     DAONQZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31334_length_3148_cov_16.623666"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1211)
                     /locus_tag="PLR13_11915"
     CDS             complement(309..1211)
                     /locus_tag="PLR13_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HQC20090.1"
                     /translation="MNIGELLGAMVQTGMSPSSSARMKNSLGGGKELDSLKDMLGDAA
                     GQSGSGGLGGLLSSVLGGDKSSGGKSGGIGDLLSGVLGGGQKGGASSSGGIGDLLSNM
                     LGDAGKAVGGNKNLAIGGLGALIGSIMGGGGKSAGGAVGGGLMALLGALAYNALKGIG
                     QAKTPIPAGLIEPGTDEEQKQLDQSSELVLRAMINATKADGKIDQNEINRIMGKFNEI
                     GVEEEGKKYLMMQLQQPMETDTLIAAAQGQPDLAAQMYAASLMAIEVDTQNEKDYLEK
                     LAEGLGLGSQVTTRIEQMVGLQPS"
     gene            1601..2749
                     /locus_tag="PLR13_11920"
     CDS             1601..2749
                     /locus_tag="PLR13_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HQC20091.1"
                     /translation="MSPKSKEEYFEVLYKRYKEASRKEKTVIIDECCAVCGHHRKHAI
                     RRLKGYKRFTKPKAKKRGKPSVYNKAPIIRPLKQIWLAANLPCSKRLKAILPLWLPKY
                     SEHFGGLAEDIIRTLWAISPSTIDRILKPIRIQYQKRGKSTTKPGTLLRKQIPINTNQ
                     WDESRPGYLEADTVAHCGDSLMGMFAYTIDFVDIATGWTEQRSVWGKGEKGVLEQIKD
                     VEKMLPFPLLGFDSDNGSEFLNHHLLRHFTDRKQPINFTRSRAYHKDDNAHIEQKNWT
                     HVRQWLGYQRLDNPKVVPLMNNLYRNEWRLFHNFFLPSVKLIEKERIGSKTIKKHDQP
                     KTPYQRIMESKYIDQSVKLALSKQLENLNPFELNNLIQMKLKKIARLR"
     gene            complement(2904..>3148)
                     /locus_tag="PLR13_11925"
     CDS             complement(2904..>3148)
                     /locus_tag="PLR13_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20092.1"
                     /translation="FKKFPALNNLKTRGEAGFSDIHHAPTHQCPFSVSVKASEKDNLD
                     PLKRGSCLFVDFYPLIDTIIPSAIFESRYDSCERSD"
BASE COUNT          849 a          808 c          678 g          813 t
ORIGIN      
        1 tccgtatcga ccagaacacc gtcattgtcc cagaaaattg tcttgatcat taaacgccgg
       61 atctccattt ttgcttgagc actttacaca tttgtgttca ttcggtcaat ctcaaccatc
      121 tgaaaatcac gatgccggaa agcgaaatgt ctgttgtctg gtgtgtagag ttggcgcaca
      181 caccaggtgg gcaggaacaa cgagaaaaat caaatcgcaa gaaatgttta tcatgcgatg
      241 acaaccattc agaataaaaa agggtgaccg aaacagagag gcacaatcca tttcgtcgcc
      301 cggaatattt atgacggctg taaaccaacc atttgttcga tacgcgtggt gacctgagag
      361 cctaatccca gaccttcggc cagtttttcc agatagtctt tctcattctg cgtgtccact
      421 tctatggcca tcagggaagc cgcatacatc tgcgccgcca ggtccggctg cccctgtgcc
      481 gcggcaatca atgtgtccgt ttccatcggt tgctgcagtt gcatcatcag atatttcttt
      541 ccttcctctt cgacaccaat ctcattgaat tttcccatga tccgattgat ttcattttga
      601 tcgatcttgc cgtcggcttt cgtggcatta atcatcgccc tcaaaaccag ttctgaactc
      661 tgatccaatt gcttttgttc ctcatccgta ccgggttcaa tcaggccggc tggaatggga
      721 gtcttggcct gaccgatgcc tttgagcgca ttatacgcca gcgctccgag cagagccatc
      781 aagcctccgc ccacggcgcc gcctgcagat tttccacctc cgcccataat ggatccgata
      841 agcgctccca gaccgccgat ggccaggttt ttgttgccgc caactgcctt accggcatca
      901 cccaacatat tggaaagtaa atctccgata ccgcctgagc tggatgcgcc tcccttctgt
      961 ccgccaccca gcacaccgga cagtaagtcg ccgataccgc cgcttttgcc gcctgagctt
     1021 ttgtctccgc ccagcacact ggaaagcaag cctccgagtc ctccgctgcc ggactgtcct
     1081 gcggcatcgc ctaacatatc tttgagacta tccagttcct tgccgcctcc aagagaattc
     1141 ttcatgcgtg cgctggaaga cggtgacata ccggtttgca ccattgctcc tagaagttcg
     1201 ccaatattca tttctgtaac ctccttatga tttcaatgct cgaaataatg aaaacgattt
     1261 aattaacctg ttcgcttccg aacaacttcg cgacgattat tttgttgacc ttctttccct
     1321 ctggaacaat tgccggtgct tctgcggtta caggttcaga gtcggattgt gcaatggccg
     1381 gtgtttgggg tgccggaacg ggaaacagcg ttacattaca catttacatg atactcttta
     1441 atcatactgc gtgattatac aataataaga aaatatatca acatttcaaa tgatggacga
     1501 attgcctcaa ataaaatgtt accgaaggtt tgcataaaag ggatacgttg cctcataaac
     1561 cgatgttacc gagaaaagat tcggaaacgg aggtttatcc atgagtccca aatcgaagga
     1621 agagtatttt gaggttcttt ataagcgata taaagaggca tctcgtaaag aaaagaccgt
     1681 tatcatcgat gaatgttgcg ctgtttgcgg tcatcatcgg aaacacgcca tccgacgatt
     1741 aaaagggtat aagcggttta caaagccaaa ggccaagaaa agggggaagc cttctgttta
     1801 caataaggca cctattatcc ggccgctgaa acagatctgg ctggcggcca atcttccctg
     1861 ttccaaaaga ctcaaggcca ttttaccctt gtggctgcct aaatacagcg agcatttcgg
     1921 cgggctggca gaagatatta tcaggactct ttgggccata tccccgtcaa ccatcgaccg
     1981 gattctcaag cctatccgga ttcagtatca gaaaagaggg aaatcaacga ccaagccggg
     2041 cacgctgctg cgaaagcaaa tccccatcaa caccaaccaa tgggatgaat cacggccggg
     2101 ttatctcgaa gccgacaccg tcgcccattg cggtgattct ctcatgggga tgtttgccta
     2161 caccattgac tttgtcgaca ttgccacggg ctggacagaa caaagatcgg tctggggcaa
     2221 aggagaaaaa ggtgttctgg aacaaatcaa agatgtcgaa aaaatgctcc cgtttccgct
     2281 cctgggtttt gattcagaca atggcagtga gtttctcaac catcatctcc tgcggcactt
     2341 tacggatcgt aaacagccca taaactttac ccgcagcagg gcttatcaca aagacgacaa
     2401 tgcccacatc gaacaaaaaa actggacgca tgtccgtcag tggctggggt atcaacgatt
     2461 agacaatccc aaggtggtcc cgctgatgaa caatctctac cgcaacgaat ggcgcctctt
     2521 ccataacttc tttctcccct cggtcaaact cattgaaaag gaacgcatcg gctctaaaac
     2581 tatcaaaaaa catgatcaac ccaaaacgcc ctatcaaaga atcatggaat caaaatacat
     2641 cgatcaatcg gtcaaacttg ctctctcaaa acaacttgaa aacctgaacc cttttgagct
     2701 aaataatctt attcaaatga agctcaaaaa gattgcccga cttcggtaac atcttttttg
     2761 aggcaacgtt ctctccactc gctcttcttt cggtaacatt tattaatgag gcaacaggtg
     2821 gattgcctgc gacgtttcaa gaggattctt gttgatcaga tacggcgtgg cgatagttat
     2881 atttggaaat tagtttttca atttcaatca ctccgttcac atgaatcata acggctttca
     2941 aaaatggcag aaggtatgat cgtatcaatc agggggtaaa aatccacaaa cagacaactt
     3001 ccccttttta gggggtcgag gttgtccttt tcagacgcct ttacgcttac gctgaagggg
     3061 cactgatgag tcggtgcatg gtgaatatca gaaaagccgg cttccccgcg tgtttttaga
     3121 ttattcaacg caggaaattt tttgaaat
//
LOCUS       DAONQZ010000141         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_31725_length_3117_cov_24.529719, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000141 DAONQZ010000000
VERSION     DAONQZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31725_length_3117_cov_24.529719"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..428)
                     /locus_tag="PLR13_11930"
     CDS             complement(198..428)
                     /locus_tag="PLR13_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20093.1"
                     /translation="MNDEIDDEILEAEMGDDALEKKMKKVESEAARILKQSSELLKAL
                     TRDSSQQEETNQVETKQEESKATAAGESIKSS"
     gene            complement(619..1551)
                     /locus_tag="PLR13_11935"
     CDS             complement(619..1551)
                     /locus_tag="PLR13_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC20094.1"
                     /translation="MPFIRPEIIRPPSEHASYYLPLTSGCSNNSCTFCAFSLTNLGLR
                     DLEDVKREIDAMSLYVNTGAWVPGQPEIVYAILQEWDGRKVFLQDGDALIYPYPKLRE
                     ALWYLNQKFPRIQRISSYATPQDILRRSVEELKALKEQKMGILYVGVESGDDETLKII
                     RKNATHDQMVEAAKKVKESGILLSVTVILGLGGTRRSKEHVAETARILTEMDPDYAGA
                     LTLTLIPGTELYNEWKKGEFELITPFESLQELKGIVENTTFTNCFFSSMHASNYYAIR
                     GTMPKDKEKVLNQLNAILSRKDPRLLRPEFMRGL"
     gene            complement(1657..2907)
                     /locus_tag="PLR13_11940"
     CDS             complement(1657..2907)
                     /locus_tag="PLR13_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20095.1"
                     /translation="MTYYILIGFCLVILFAYFFDITSKHTKIPSALILILIGMALNYV
                     AGYFELHIPYMDRILPVMGTLGLILIVLEASLDLTLSREKRLIIMGASSSAVLLFLVF
                     VGLLAFVAVSCFDYDIKTCIINIIPIAVISSAIAIPSAMGLSRTDREFVTYESSLSDI
                     LGILAFNFILLFNGSLAAGIFSFFAELILTLLASIIFSAFLAYMLHKINHHVKYVIIM
                     TAIVLVYALANLIHLPSLFVVLIFGLVMNNNLLFKNKFSEKIICFEEFNEDLNSFKNI
                     TAELNFIVRSFFFLLFGFFTSFYDLISLSNLKFAVGICAIIFLLRALYFKTVLRLPLN
                     PLFYFAPRGLITILLFLSIPAGLLLPFMNVGIVTQVIFITILMMTFGSIIFKKVDELV
                     TPLSKKEKGPCRDKNMSKDKIGTV"
BASE COUNT          839 a          649 c          744 g          885 t
ORIGIN      
        1 cgggcagtta atgaacaaat ccagcttcga tctgctcagg cgaaaaggtt tctgagcttt
       61 cgggtcataa agattttttg tccgacccgg ggtgtaatat ttcgacatga ttatcctccg
      121 agacattctc caaagttgcc ggtggctttg tctgttttaa ttatcctgcg aatttccact
      181 taccacggtt atttcctcta cgatgatttt atgctttctc ctgcggctgt cgctttgctt
      241 tcttcttgct ttgtttcaac ctgatttgtt tcctcctgtt gtgaagagtc tctggtgagc
      301 gccttcagta attccgagga ttgttttaat attcgtgcgg cttcgctttc tacctttttc
      361 attttctttt ccagggcatc atctcccatc tcggcttcta agatttcatc atcaatttca
      421 tcgttcatga taaaactcct tcttgtcttg tgaaaaaagc aacatgaatc aaagccctaa
      481 acgcgttcat ttttttgttg atgtattttt atgtatttaa aggaattttg caagcaaaag
      541 gaaaagcttt cgtacggccg ttatttgttc cggtgccttc catgatggaa gagaacgaaa
      601 gggagccgct tctgttgttt acaaccctcg cataaactcc ggccggagca ggcggggatc
      661 tttgcgtgac aatatggcgt ttaactggtt cagcaccttt tctttgtctt tgggcatcgt
      721 gccgcgaatc gcgtaataat ttgacgcgtg catggagctg aaaaaacagt tggtgaaagt
      781 tgtattttcg acaattcctt tcagctcttg cagagattcg aacggtgtaa tcagttcaaa
      841 ctcgcctttc ttccattcat tataaagttc cgtcccgggg attaatgtta atgtcagagc
      901 tcccgcgtag tccgggtcca tttccgttaa aattcttgct gtttccgcga cgtgctcttt
      961 gctgcgacgg gttccgccca gtcccaggat aaccgttacg gacagcagga tgccggattc
     1021 ctttactttt tttgcggctt ccaccatctg gtcgtgagtg gcgtttttcc gaattatttt
     1081 gagcgtttcg tcatcaccgc tttccacgcc cacataaagg attcccattt tttgctcttt
     1141 gagcgctttt aattcttcga ccgagcgccg gagaatatcc tggggtgtgg catagctgga
     1201 gatgcgctgg atacgcggaa atttttgatt aaggtaccac aaagcttccc gcagtttggg
     1261 gtagggataa atcagggcgt cgccgtcctg aaggaaaact tttctgccgt cccactcctg
     1321 cagaatcgcg tacacaattt cgggctgtcc gggcacccac gcgcctgtgt tgacatatag
     1381 agacatggca tcgatttccc tctttacatc ctccaggtcg cggagtccga gatttgtcag
     1441 agaaaacgcg caaaaggtgc aagaattatt tgaacacccc gacgtcaggg gcagatagta
     1501 gctggcgtgt tcgctggggg gcctgatgat ttccggtctg ataaacggca tagtttcacc
     1561 ttctatataa gtaattttct aataaggtat aagcgtaagt cacgctttgt caagaggatg
     1621 tcttgcccaa gaggaaaatg taagcacgct ggtaaattaa acggtaccaa ttttatcttt
     1681 ggacatattt ttgtcccggc aaggtccttt ttcttttttt gataggggcg tcaccaattc
     1741 atcaactttt ttgaagatta tgctgccgaa ggtcatcatc agaatggtta tgaaaattac
     1801 ctgtgttacg attcccacgt tcataaacgg caaaagtaat ccggcgggaa tgctcaaaaa
     1861 cagtaaaatg gtgattaatc cgcgcggcgc gaagtagaaa agggggttca gcggcaggcg
     1921 cagcacggtt ttaaaataca gagcgcgcag aaggaaaatt atcgcgcaga tgccgacggc
     1981 aaatttcagg ttgcttaagc ttatcaggtc gtaaaaactg gtgaagaagc cgaagaggag
     2041 aaagaaaaag gagcggacga taaagttcag ttccgcggta atgtttttga aggaattcaa
     2101 atcttcatta aattcctcaa aacagattat tttttccgag aatttatttt tgaaaagcag
     2161 gttgttgttc atgaccaggc cgaaaatcag gacgacaaac agcgaaggca aatgtatgag
     2221 gttggccaaa gcgtaaacca gaacgatggc ggtcatgatg atgacgtatt ttacgtgatg
     2281 gtttattttg tgcagcatat aggccagaaa ggcgctgaat atgattgaag caagcagcgt
     2341 aagtatgagt tcggcaaaaa aactgaatat ccccgcagcc aaggagccgt tgaataataa
     2401 aataaaatta aaggccagaa taccgagtat gtccgaaagc gagctttcgt aagtgacgaa
     2461 ttcgcggtcg gttcgggata atcccatcgc ggaaggaatg gcaatcgcgc tgctgatgac
     2521 ggcgatggga atgatattta taatgcatgt ttttatatcg taatcgaaac aagagacggc
     2581 gacaaaggcc aacaagccca caaaaaccag gaaaagcaaa accgccgaag atgaagcgcc
     2641 catgataatc aagcgttttt ccctgctgag cgtcaaatcc agactggctt ccagcacaat
     2701 cagtatcagg ccgagcgttc ccatgacggg aagtattctg tccatatatg gaatgtgcag
     2761 ctcaaaatat ccggcaacgt aattaagcgc cattccgatg aggataagta tcaatgcgct
     2821 gggaatcttt gtgtgcttgg atgtgatgtc gaaaaaataa gcaaagagga taacgagaca
     2881 gaagccaatg aggatataat atgtcatttt taaatgcttt cttgttgttg gagggtattc
     2941 tatcactaaa gtacggcggg atgaagtatt aaatgtgaaa atgttgcggg caagggatgt
     3001 ttacatcaac ttgccacaca tggaattgac accgtgctct tttcatatta ggatgatgcg
     3061 acaaaaaagg cgggagtaaa atgacggaat ttaagtcggt aacccccaga gaagtta
//
LOCUS       DAONQZ010000142         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_32893_length_3035_cov_16.232215, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000142 DAONQZ010000000
VERSION     DAONQZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32893_length_3035_cov_16.232215"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLR13_11945"
     CDS             complement(<1..782)
                     /locus_tag="PLR13_11945"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQC20096.1"
                     /translation="MMITGKKKKSIGRSPGIFLAVFFMLWMVLPVHESWAATGYPKSR
                     GAVNDFAGVIDAENTAKMEALAQEVLQKTGTAVVVATVETIGENEDYNMYANGLYQAW
                     GIGKKGEDKGVLIFLTVKERKIRIETGYGVEGILPDGRVGEILDTYVVPFLKDGHYGK
                     GLYNAMYACSAYIAREAGVQLSGSPMPYRANAQLTQRKITVFEILFFLIAAAVLLGTR
                     RGREMLPWLLVVLMSGGRGGGGGGFGGGFGGFGGGMSGGGGAG"
     gene            933..2483
                     /locus_tag="PLR13_11950"
     CDS             933..2483
                     /locus_tag="PLR13_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC20097.1"
                     /translation="MTPKIILIHPPVAKCSEPPAGIARLASCLNANAIPYETIDANLE
                     GLLYLLKSASGNNTLSDRWTKRACKNLDRHLAAMKNIGTYQNPGRYGQAVKDINRLLN
                     VAAKPFHVALSLTDYRDRRFSPVKSADLIASAHNPQANPFYPYFKNRLTNALWSNPDY
                     IGLSLNYLSQALCTFAMIGFIKNIHPRQKIILGGSLVTSWMKLGAHLHTFGGLVDEIV
                     EGAGEQKLLEILGTQNKLIHNAPAYDDFPLDQYLSPGLIIPYSGARGCYWRQCAFCPE
                     KAEGNAYHPLEASQANAELQELTRQMNPTLIHLLDSSISPSLLQTLSQNPPGAPWYGF
                     VRVTAHLADEDFCLSLKKSGCAMLKLGIESGDQNVLDQLHKGIDLLTASAVLKNLKKA
                     GIATYCYFLFGTPPENENSAFTTMNFIADHRDCIDFLNLAIFNLPVRSVESDNLATHD
                     FYAGDLSLYRNFKHPLGWHRPAVRRFLESVLKKHPAISLILKRNPVFFTSNHAPFFAL
                     NGLSHEHF"
     gene            2644..>3035
                     /locus_tag="PLR13_11955"
     CDS             2644..>3035
                     /locus_tag="PLR13_11955"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HQC20098.1"
                     /translation="MIFNDITVSSLILFFAVYACMGWVIEVIYRSVSQRQLINAGFLF
                     GPFIPIYGFGAVFIILLEFLIHPWPLPVKIAFYGIILTGIEYFTSYIMEKAFKLKLWD
                     YSNNKFHIHGRVCLLFSIFWTAMALGFIT"
BASE COUNT          770 a          809 c          649 g          807 t
ORIGIN      
        1 ccggcgccgc cgccgccgct catgccgccc ccgaaccctc cgaaaccgcc gccgaaaccg
       61 ccgccaccac cgccgcgtcc gccgctcatc aatacaacca gcagccaggg aagcatttcg
      121 cggccccgcc gggtacccag aagcactgcg gcggcaatta aaaagaaaag aatttcaaaa
      181 acggttatct tcctttgcgt caactgagca tttgcccggt aaggcatcgg cgaaccgctc
      241 aactgaacac cggcctctct ggcaatgtat gcagaacagg catacattgc gttataaagt
      301 cccttgccgt aatgtccgtc tttcagaaac ggcacaacat acgtatcaag aatttcgccg
      361 acccgtccgt ccggcagaat gccttccacg ccgtaaccgg tctcaatgcg gattttccgt
      421 tctttgacgg tcagaaaaat taatacgcct ttatcttcgc cttttttgcc gatcccccag
      481 gcctgataaa gcccgttggc gtacatattg taatcttcat tctcgccgat ggtttcaacc
      541 gttgccacga caactgcagt gccggtcttt tgcaaaactt cctgagccag cgcctccatt
      601 tttgcggtgt tttccgcatc aatgacaccg gcaaaatcat taaccgctcc gcgcgacttc
      661 ggatacccgg tcgccgccca tgattcatgg acgggcagaa ccatccaaag catgaagaag
      721 acggcaagga aaatccccgg agaacggccg atacttttct ttttcttgcc agtaatcatc
      781 atgtgtctgt cagagcggta gtttattaat attacgaact attgcttaaa gttttacggc
      841 tgtcaagttc aaagcagaaa gccaaaatcc ttcttgcgta tgcgcagatt acgtactaga
      901 aaaaaacagt ttttcaaagg taaggtttta ttatgacgcc taaaatcatt ctgattcatc
      961 cgccggttgc aaaatgttcg gagccgcccg cggggatcgc cagactggct tcctgtttga
     1021 atgccaacgc catcccatat gaaacgattg atgccaatct ggaaggtctt ttgtatctgt
     1081 taaagagcgc ttcgggaaac aacaccttat ccgacagatg gacaaagcgc gcgtgcaaaa
     1141 atctggatcg tcatcttgct gcaatgaaaa acatcggcac gtaccaaaat ccgggtcgct
     1201 atgggcaggc cgtcaaggac atcaaccgtc ttttaaatgt tgcggcaaaa ccgtttcatg
     1261 ttgccttgtc actgaccgat taccgggaca gaagattctc tccggttaag agcgccgatc
     1321 tgatagcgtc cgctcacaat ccgcaagcca acccctttta cccttatttt aaaaatcgtc
     1381 tgacgaacgc cctttggtca aatcctgatt acataggctt gtctttaaac tacctgagtc
     1441 aggcgctctg cacctttgcc atgatcggtt ttataaagaa cattcatccg cgacaaaaaa
     1501 ttattctggg cggatcgctt gtcacatcgt ggatgaagct gggcgcacat ctccatacct
     1561 tcggcggtct ggttgacgaa attgtggaag gcgcaggaga gcaaaaactg ctcgagatac
     1621 tgggtacaca aaacaagctg attcacaacg cccctgccta cgatgatttt ccacttgatc
     1681 aatatttatc gcccggcctg atcatcccct acagcggcgc gcgcggctgt tactggcgcc
     1741 aatgcgcttt ctgcccggaa aaagccgaag gaaatgctta tcaccctttg gaagcctctc
     1801 aggccaacgc agaactgcag gaattaaccc gccaaatgaa tccaacgctg attcatcttc
     1861 tggatagttc tatttcgccg tcgctcttac agactctgtc acaaaatccg cccggggcgc
     1921 cctggtacgg ctttgtccgg gtcactgcac atttagctga tgaagatttc tgtttatctt
     1981 taaaaaaaag cggctgtgcc atgctgaagc tgggtattga atccggcgat caaaacgttc
     2041 tggatcaact ccataaagga attgatttac tgacagcctc tgccgtttta aaaaatttga
     2101 aaaaagcggg gattgccacg tattgttact tccttttcgg cacgccgccg gaaaatgaaa
     2161 atagcgcatt cacaacaatg aacttcattg ccgatcatcg cgactgtatc gattttctga
     2221 atctggccat tttcaatttg ccggtccgga gtgtcgaatc ggataatctg gcgacgcatg
     2281 atttctacgc cggcgacctg tctctttatc gaaacttcaa acaccccctg ggctggcaca
     2341 gacccgccgt tcgtcgtttt cttgaaagcg ttttaaagaa acacccggcc atctctttga
     2401 tccttaaaag aaatccggtt ttttttacgt ccaaccacgc cccttttttt gctctcaacg
     2461 gattgtcaca tgagcatttt tagcaaaata tcccctgcag agacttgttg acaacgcttg
     2521 tccaatatta taggtatggc gactaatccg ctccggattg acatgacata aaccgatgaa
     2581 cttaagcctc cgaattcaaa taacacactg ctgcaagacc cgaacatgag ataaatatat
     2641 ccaatgattt ttaacgatat aacggtatcc agcctcattc tgttttttgc tgtctatgct
     2701 tgcatgggat gggtcatcga ggtcatttac agatccgttt cacagcggca gctgatcaat
     2761 gcaggctttc ttttcggtcc ttttattccc atttacggtt tcggagcggt ttttattatc
     2821 cttctggaat ttctgattca tccgtggccg ctgccggtca aaattgcctt ttatggaatt
     2881 attttaacgg gcatagagta tttcaccagc tatatcatgg aaaaagcttt caaactgaag
     2941 ctctgggatt attcgaacaa caaatttcat attcatggcc gcgtctgtct gcttttctcc
     3001 atattctgga cggcaatggc tttgggattc atcac
//
LOCUS       DAONQZ010000143         2965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_33990_length_2965_cov_7.880756, whole genome shotgun sequence.
ACCESSION   DAONQZ010000143 DAONQZ010000000
VERSION     DAONQZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33990_length_2965_cov_7.880756"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1071
                     /locus_tag="PLR13_11960"
     CDS             106..1071
                     /locus_tag="PLR13_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20099.1"
                     /translation="MDKFTSRIVETLLQLGADLVGIAPVERFAHAPEGHRPADFMPEC
                     KSVISIGLHIHQGAADVWGEHDDKSKSSTPYLFYGYGLTNIESSRIVNRVAKLIEYQG
                     YQSLCFLPTWLTSMTKYMDESLVEGKLRAEFSHRHAAVAAGIAEFGWNGLALTPEFGA
                     MQRFNSILTSAELEPTSLYNGPQLCRKEKCGAMCVKTCPTKAIAPETKQVLRIGDRTF
                     EYAAHDNVRCIYAIHGMVKGSGGRSDIKIPDGPVSFIDFYAAKSGTEMNYYDKSMLEN
                     CFGIICGDFCGRCLHKCPSRKIAQKDLKHYDFTNRFSVMNNLQFR"
     gene            1094..1675
                     /locus_tag="PLR13_11965"
     CDS             1094..1675
                     /locus_tag="PLR13_11965"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 family protein"
                     /protein_id="HQC20100.1"
                     /translation="MRKHQILTFIGIIGAAAWFTVCDKFFHIDNNILVYYWQPLNVLF
                     QGQSAWTFPLFACATTSFFFATLPFASAWREHPSYLILTVNTIVCTLIYFGSGIMGNS
                     HPMIYFWIVFVAWIIRIIFERENRTTAIKACLVMGLLGCFWEGATSKLGYFEYYYTNI
                     FSVPLWLFACYLHAGFLMLEMQSFREFFKRSSK"
     gene            complement(1723..>2965)
                     /locus_tag="PLR13_11970"
     CDS             complement(1723..>2965)
                     /locus_tag="PLR13_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQC20101.1"
                     /translation="NWLYQTRDIMFQIKEWLGVEKLLSLDAYKEYYGMDDINSFLDVN
                     FKVCRDVMCPANKDADEPGAKFVGGNEHAVVTPDSFKNVYRTVMDAELGPQFGYRGEG
                     KSEMSRTFLSNVGRLVEQYKKPVAVTIITDAPALLNISRNQGYPLFTTPLMAAKALHA
                     SATYYEEKTARNARGEIVSTLIDSEAINKIKKLCKTEKRIPLTDEALQIAAAADIRCI
                     SGVTVKSATEADNFKIDFPVVVKLLSRDASHKSDVGGVQLNIKNHKELALTISGMKKA
                     FQEISPQPAIDGFLVQKMAEEGVECFVGGRQDPVFGPIVIAGLGGVFLEIFKDTAIRL
                     APITKNEAMDMLKNLKTYPVLQGARGKIKADIDALADVICKVSNLLAKVPDIAEIDLN
                     PVIVHETGKGVSIVDSRVFFK"
BASE COUNT          798 a          647 c          667 g          853 t
ORIGIN      
        1 gtttatttta gcgacctgtc gcccgaactg caggatgaaa taatcgcccg caccagtaac
       61 aagacatgct gaaccgaaaa taatttattc tgataggaga acaatatgga taaatttacc
      121 agtagaatcg ttgagactct gctgcaactt ggcgccgatt tggtaggtat cgcaccggtg
      181 gagaggttcg cccatgcgcc ggaaggacat cgaccagctg attttatgcc ggaatgcaaa
      241 tcggttatat ccatcggact gcacatccac cagggcgcgg ctgatgtgtg gggagaacac
      301 gacgataaaa gcaaatcaag cacgccttat cttttttacg gatacggatt aacaaatatc
      361 gaaagttcac gcatcgtcaa ccgcgttgcg aaattaattg aatatcaagg atatcaaagc
      421 ctttgttttc tgcctacgtg gttaaccagt atgactaaat atatggacga aagccttgtt
      481 gaaggaaaat tgcgtgccga gttttcgcat cgtcatgcag cggtagcggc gggaatcgcg
      541 gaattcggat ggaatggact ggcattgaca cctgagtttg gagctatgca gagatttaat
      601 tccattttga catcagcgga actagaaccg acatctcttt acaacggacc gcaattgtgc
      661 cggaaagaga aatgtggagc gatgtgcgta aaaacgtgtc caacaaaggc gattgctcct
      721 gaaacaaaac aggttttgcg tataggagac agaacatttg aatacgcagc ccatgataat
      781 gtacgttgta tatacgccat tcatggcatg gtaaaaggtt ccggcggaag aagcgatatt
      841 aaaattcccg atggacctgt atcttttatt gatttctacg cggcaaaatc cggtacagaa
      901 atgaattatt acgataaatc aatgctcgag aactgtttcg gaattatctg cggcgatttc
      961 tgcggtcgtt gtctgcataa gtgtccctca cgtaaaatcg cgcaaaaaga tttaaaacat
     1021 tatgatttca ctaatcgatt tagtgtaatg aataatttgc aatttcgtta gataaaatat
     1081 accgggggct tgtatgcgaa aacatcaaat actcacgttc attggcatta tcggcgccgc
     1141 ggcatggttc accgtgtgcg acaaattttt tcacattgat aacaacattc ttgtttacta
     1201 ctggcaaccg ctgaatgttc tatttcaggg acaatctgcg tggacatttc ccctgtttgc
     1261 ctgcgcaacg accagctttt tctttgcaac acttccgttt gcaagtgcat ggagggaaca
     1321 tccgagctat cttattctga ccgttaatac cattgtgtgt accctgatat atttcggttc
     1381 aggtataatg ggaaattcac atcccatgat ttatttttgg atagtatttg tggcctggat
     1441 tattcgtata atttttgaac gtgaaaatcg cactacggcc ataaaggcat gcctggtaat
     1501 gggattgctc ggttgtttct gggaaggagc tacatccaag cttggatatt ttgaatatta
     1561 ctatacaaat atattcagtg tccccttgtg gctttttgcg tgctacctgc atgccggttt
     1621 tctgatgctg gaaatgcaga gcttcaggga attttttaag cgttcaagca aataggggct
     1681 gcaattaaag gaatttgtcg gacaaaaaaa ctttctttat tgtcatttga aaaatacccg
     1741 gctgtcgaca atagaaactc ccttgcctgt ttcatgaacg ataaccggat tcagatcgat
     1801 ttcggctata tcaggcactt tcgccaacag gtttgatact ttgcatatta catcggctaa
     1861 ggcatctata tccgctttta ttttacctct cgctccctgc aataccgggt atgtcttcag
     1921 atttttcagc atgtccatag cttcgttctt tgttatcggc gcaagacgga tagccgtgtc
     1981 tttgaatatc tcgagaaaaa cgccacccag accggcaata acaatggggc cgaaaacagg
     2041 atcctgacgt ccgccaacaa agcactctac gccttcttca gccatcttct gaaccaggaa
     2101 accgtcgata gccggctgag gagagatttc ctggaaagcc tttttcatgc cggatatggt
     2161 cagagcaagc tctttatgat ttttaatatt taattgcacg ccacccacat cggatttatg
     2221 ggaagcatca cgggaaagaa gttttaccac aacagggaaa tcaatcttaa agttgtctgc
     2281 ttccgttgcc gatttaacgg taacaccgga aatacatcgg atatccgcgg ctgcggcaat
     2341 ttgcagagct tcatcggtca gcggaatccg tttttcagtt ttacaaagtt ttttaatttt
     2401 gttaattgcc tccgaatcga taagtgtgga aacgatttct ccccgggcat tcctggcagt
     2461 tttctcttca tagtatgtag cagaagcatg gagagctttt gccgccatca gaggagtagt
     2521 aaaaagcgga tatccctggt ttctcgatat attaagaagt gccggtgcat cggtaattat
     2581 tgttaccgca accggttttt tatattgttc cacgagcctg ccgacattgc ttaggaaagt
     2641 acgcgacatt tcacttttgc cttcgccgcg gtaaccgaac tggggtccca gttccgcatc
     2701 catcacggtt ctataaacat ttttgaaaga gtcgggggtt accaccgcat gttcattacc
     2761 gcccacaaat ttcgcgccgg gttcatcggc gtctttgttg gccgggcaca tcacgtcgcg
     2821 gcaaacttta aagttgacat cgaggaagct gttgatgtcg tccatcccgt agtattcttt
     2881 gtaagcatca aggctcaaga gcttttctac gccgagccat tctttgatct ggaacatgat
     2941 gtcccgcgtc tgatacaacc aatta
//
LOCUS       DAONQZ010000144         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_35595_length_2862_cov_14.320271, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000144 DAONQZ010000000
VERSION     DAONQZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35595_length_2862_cov_14.320271"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLR13_11975"
     CDS             complement(<1..828)
                     /locus_tag="PLR13_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HQC20102.1"
                     /translation="MKVSYYPGCSLHATGKEYDQSMKAVSRALDIELKEVDDWCCCGA
                     SSAHNTNFDLSIALPARNLIAAEKDAMDVMVPCAACFNRFKTAEHHLKADKDLKAKIE
                     GIVGAKYQGGIAIRNPIDIIFNDIGIDVLAEKVVKPLTGLKPVSYYGCLLLRPPEVCE
                     FENYENPFMLDKMMKAIGADVKNWSYKTDCCGGSLAISKTAIVVEMCDRLMNGAREAD
                     ANCVVTACPLCMANLDMRPSEGKKLPVFYFTELISLAMGLPGSKDTFKSHIFDPRPLL
                     "
     gene            complement(856..1380)
                     /locus_tag="PLR13_11980"
     CDS             complement(856..1380)
                     /locus_tag="PLR13_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC20103.1"
                     /translation="METFLEEVNKKINGVPLQRCYHCRKCTAGCPMTKHMEYNPNKII
                     KMIQNGQRDKVLNSSTIWLCLSCETCITRCPNQVDIASMMDVLRQMAIQQGTVREANV
                     LKFHEAFLAGMKFGGRINEPMMMVQYKLKSGDLFSDALLGLDMFKKGKLALLSPRTKD
                     MKSVRNIFEKTKNR"
     gene            complement(1405..2415)
                     /locus_tag="PLR13_11985"
     CDS             complement(1405..2415)
                     /locus_tag="PLR13_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQC20104.1"
                     /translation="MENIETKLREEANRLLLEKKVDVIVGYEQGTLPLTATPCFITKP
                     EETNKLVWNNLCTQNLAKYVHDLITQHKNSQKRVKPEDRKKKIVGVVARGCTTRSLII
                     HLQERQYGRDEVYIIGVPCDGYVDGKGLKVAIGGADIVDGSIDGGQIKMKTSGGDKTV
                     ALKDVLADNCKTCRFNNPIISDVMIGSPAPAMNPDAEYDDVKEFEGKSIEERWAYFNK
                     EMEKCIRCYACRQACPSCYCPTCFVEQSQPQWVGIGEDASDTQVFQFMRLYHMVGRCV
                     DCGSCISVCPMGVDLRKFLKKLDKDCFEFFGNRAGSSMDDMPPLGKFDEHHDKGDFVY
                     NP"
     gene            complement(2453..>2862)
                     /locus_tag="PLR13_11990"
     CDS             complement(2453..>2862)
                     /locus_tag="PLR13_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_except=(pos:complement(2675..2677),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HQC20105.1"
                     /translation="PKFVAIVCNWCTYAGADLAGISRIQYPTNVRIIRVPCTGRINPF
                     YIVKALQAGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLNYIGVEGDRTIFTWV
                     SASEGDRWAQVIKGATEKIKALGPANKLIKKID"
BASE COUNT          670 a          760 c          654 g          778 t
ORIGIN      
        1 caacagcggc cggggatcaa aaatatgcga tttgaacgta tctttcgatc cgggaagccc
       61 cattgccaac gatatcaatt ccgtgaaata aaatacaggc agtttctttc cttcgctggg
      121 acgcatatcc agattggcca tacacagcgg acacgccgtg acgacgcagt tcgcatccgc
      181 ttcacgggcg ccgttcatca accggtcaca catttccacc acaatcgcgg ttttactgat
      241 cgccaatgaa ccgccgcagc aatcggtctt atacgaccag ttcttaacat ccgcgccgat
      301 ggctttcatc attttgtcca gcataaacgg attttcgtaa ttttcgaact cacaaacctc
      361 gggcggtcta agcagcaggc acccgtaata ggaaacaggt ttcaatccgg tcagaggttt
      421 aaccaccttc tcggcaagca cgtcgatacc tatatcgttg aaaatgatgt cgatgggatt
      481 acggatggca atgccgccct gatacttcgc gccgacgata ccttcaattt tcgccttgag
      541 atccttatcg gcttttaaat ggtgttcggc tgttttaaaa cggttgaaac aggcggcaca
      601 gggtaccatg acatccatcg catctttttc tgcggcaatc aggtttcgcg ccggcagagc
      661 aatggacaaa tcaaaatttg tattgtgcgc ggacgatgcg ccgcagcaac accagtcatc
      721 aacttctttg agttcgatgt cgagggctcg actgacggcc ttcatcgact gatcatactc
      781 ttttcccgtc gcgtgcagcg agcagcccgg ataatatgaa actttcaaga gtagcctcct
      841 taacctaaaa cctctttatc tgttttttgt tttttcaaaa atatttctaa ccgatttcat
      901 gtctttcgtg cggggtgaca aaagggccag ttttcctttc ttaaacatat ccagacccag
      961 cagcgcatcg gagaataaat ctccggactt gagcttgtac tgaaccatca tcatcggttc
     1021 gttgatgcgt ccgccgaatt tcatgccggc caggaatgcc tcatggaact tcaaaacatt
     1081 cgcttcgcgg acagtgccct gctgtatggc catctgcctg agcacatcca tcatactggc
     1141 aatatccacc tgattggggc agcgggtaat acaggtttca caggacaggc aaagccagat
     1201 ggtggaactg ttgagcacct tgtctcgctg gccgttctga atcatcttga tgatcttgtt
     1261 ggggttgtac tccatgtgct ttgtcatggg acagccggcc gtgcatttgc ggcaatgata
     1321 gcagcgttgc agcggcaccc cgtttatctt tttgtttact tcttccagaa aagtttccat
     1381 gcaaactcca ttatctcaag ctaattatgg gttgtagaca aagtctccct tgtcgtgatg
     1441 ttcatcgaat ttgcccagag gcggcatatc gtccatactg gatcctgcgc ggttgccgaa
     1501 gaattcaaag caatccttgt cgagtttctt gaggaacttc cttaaatcca cgcccatcgg
     1561 acagacggaa atgcacgacc cgcaatcaac acagcgtccg accatgtgat acaatctcat
     1621 aaactggaaa acctgtgtat cggaagcgtc ttcaccgatg cccacccact gcggctgact
     1681 ttgttcgaca aaacaggtcg gacaatagca ggaaggacag gcctgacggc aggcataaca
     1741 gcggatgcat ttctccattt ccttgttaaa atacgcccag cgctcctcaa tggatttgcc
     1801 ttcgaattct ttgacgtcgt catattcggc gtcgggattc atcgctggag cgggagaacc
     1861 gatcatcaca tcggaaataa tgggattgtt gaaccggcag gtcttgcagt tatccgccag
     1921 aacatccttg agcgcaaccg ttttatcacc gccggaggtt ttcatcttga tctggccgcc
     1981 gtcaatcgat ccgtccacga tatccgcgcc gccgatggcg accttcaatc ctttgccgtc
     2041 cacatatcca tcgcagggaa cgccgatgat atacacttca tcacgtccgt actgtctttc
     2101 ctgaagatga atgatcagag aacgagtggt gcaaccgcgc gccacgaccc cgacgatttt
     2161 tttcttccgg tcttccggtt tgactcgttt ttgcgaattt ttatgctgcg taatcaggtc
     2221 gtgcacatat ttggccagat tctgtgtgca gaggttgttc cagaccagtt tattggtttc
     2281 ttccggcttg gtgatgaaac aaggcgttgc cgttaacggt aaggtgccct gttcataacc
     2341 aacaatgaca tcaacctttt tttccagaag cagcctgttt gcttcttccc gtaattttgt
     2401 ttcgatgttt tccacagtaa ttgtttcccc gttatttttt tattgcaaaa atttaatcaa
     2461 tcttttttat caatttattc gccggtccca gcgccttgat tttctccgta gcgcctttga
     2521 tgacctgcgc ccatctgtca ccttcggagg cggaaaccca cgtaaagatg gttctgtcac
     2581 cttccacacc gatgtagttc aggaaacttt ttaacgtcgc aaacttgcgc cgagccacca
     2641 gatttcccga gatatagtgg cattcaccgg ggtgtcaacc cgacaccaga acgccgtctg
     2701 cgccggcctg cagtgctttc acgatataaa agggattgat tcgtcccgtg cagggtacac
     2761 ggatgatccg gacatttgtc ggatactgaa ttctgctgat acccgccaga tcggcgcccg
     2821 cgtaggtgca ccagttgcag acgatggcaa cgaatttcgg tt
//
LOCUS       DAONQZ010000145         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_36825_length_2790_cov_17.709324, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000145 DAONQZ010000000
VERSION     DAONQZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36825_length_2790_cov_17.709324"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLR13_11995"
     CDS             <1..295
                     /locus_tag="PLR13_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20106.1"
                     /translation="LKMAARDIGFMFSRRVFHKDLQLNNILLNPADPAQLHWIDNDLK
                     KIGRRRWAKQPKAVLEQITRNVQFCSIDEKDFFIKEIVSSWAIQKEEDDGQPS"
     gene            complement(310..999)
                     /locus_tag="PLR13_12000"
     CDS             complement(310..999)
                     /locus_tag="PLR13_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HQC20107.1"
                     /translation="MTAFCRRFVLAILMVAASTGPAWSAADDGWKKVGESDGIAGFTR
                     PTAKSSVDEIRAVGVVNAPVAVVEAVIRDISIMPQYMFLCKEAALINTPEMKSAGDAV
                     YFHSVTDMPFPVSDRDAVVKSVWSVDRKTGAVYCRAKGLKTTFRQNKDIVRMPLLIIE
                     CTLVPKGADKTEVIYQVLGDPGGELPSWLVNMLTSDYGIKTIAGLRKMVSREKFRNAQ
                     KVITVTPHDQK"
     gene            complement(1039..2400)
                     /locus_tag="PLR13_12005"
     CDS             complement(1039..2400)
                     /locus_tag="PLR13_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQC20108.1"
                     /translation="MKIALVRPNYKSHIITPPIGLGYLSSYLKKSGIETKIIDGLKEN
                     LSPSRLMRRILKFNPDAVGITCLTAFYQEVVVLARELAKNKITTVIGGQHPTFLPYRT
                     LLESQADFVVCGEGEIPLLELAESKFCNKSIRGVYSLENLKSEQQEIIKASVVDNLDD
                     LPFPDWEQIDPREYPRAPHGAVVRGFPVGVMVTSRGCPFECTFCAAPRFCDRTIRFRT
                     PENVVAEIDYLMKNFNISEVHFEDDNFTLNADNVKKFCRLLIEKNIKIHWACPNGVRA
                     DHISEDLIVLMKQSGCYYIAYGIESVNSSILEKIKKRESVETIQRAIDMTGKAGISTQ
                     GFFIFGLPGETAASMEDNIRFALRSGLSRAQFVILDVLPGSELWDSLRGKFTPNWSKE
                     SFRQPEYLPEGMTKEQLMQAQSKAFRKFYLRPQILFRLLSLMDYRQIFYLLRRLKTYR
                     ILG"
     gene            complement(2397..>2790)
                     /locus_tag="PLR13_12010"
     CDS             complement(2397..>2790)
                     /locus_tag="PLR13_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20109.1"
                     /translation="FWEKRDTCDFIIGWRARRADGPARVFISRILKGVLALLFNVSLK
                     DANAPFRLMKAERLRLYLPLIPDNFFIPNVLLSVMLTREGEKILWQEISFNPRNAGQS
                     SIALFRFGGLGIKLIIQLYKLRHLKRST"
BASE COUNT          701 a          732 c          650 g          707 t
ORIGIN      
        1 cctgaaaatg gcggcgcggg atattggttt catgttctcc cgccgggtct ttcacaagga
       61 cctgcaactg aataatattc tcctgaaccc tgccgatcct gcacaactgc actggataga
      121 taatgatctg aaaaaaatcg gcaggcgtcg ttgggccaaa caaccgaaag ccgtccttga
      181 acaaataaca aggaatgttc agttttgttc gattgatgaa aaagatttct ttataaagga
      241 aattgtttca tcatgggcaa tccaaaaaga agaggatgac ggacagccgt cctgaaaaac
      301 agccatcgtt catttttgat catgaggcgt tacggtaatc accttttggg cattcctgaa
      361 cttttcccgc gacaccattt tcctgaggcc ggcgatcgtt ttgatgccgt aatcgctggt
      421 cagcatgttg accagccagg aaggcaattc acctccggga tcgccgagga cctgatagat
      481 cacttccgtc ttatccgcac ctttcggcac cagcgtgcat tcaatgatca aaagcggcat
      541 ccgcacgata tccttatttt gcctgaatgt tgttttcagt cctttcgcac ggcaatacac
      601 ggcccccgtt ttcctatcga cagaccatac ggatttgacg acagcgtcac ggtcgctgac
      661 ggggaaaggc atgtcggtga cgctgtggaa atagacggca tctcctgcac ttttcatctc
      721 cggggtattg atcagggccg cctctttgca tagaaacata tactgcggca tgatggaaat
      781 atcgcggata acggcctcga ccaccgcgac cggagcattc acaacaccga ccgcccttat
      841 ttcatcaaca gaactcttcg ctgtcggacg cgtaaaaccc gcaatgccgt ccgattctcc
      901 gactttcttc cagccgtcgt cggctgcaga ccacgccggc ccggtactcg ccgccaccat
      961 caaaatcgcc agtacaaacc gtctgcaaaa tgctgtcata gttatttccg cttcacgcaa
     1021 tcattctttc ccgccattct atccgagaat tctataggtt tttaatctcc tgagcaaata
     1081 aaatatctgt ctgtaatcca ttaaagaaag aagtctgaac aagatttgcg gtctcaaata
     1141 aaatttccgg aaggccttgg attgcgcctg catcaattgt tctttggtca ttccctccgg
     1201 aagatattcc ggttggcgaa aactctcttt tgaccagttc ggcgtgaatt tgccccgcag
     1261 actatcccac agctccgacc cgggcagaac atccaggatg acgaattgcg ctcttgacaa
     1321 tcccgacctt aaggcaaaac ggatattgtc ttccatcgag gccgccgttt ctcccggcag
     1381 cccgaaaatg aaaaaaccct gcgtggaaat gccggctttg ccggtcatat cgatggcgcg
     1441 ttggatggtt tcgaccgact cccttttctt gattttttcc agaatggacg aattaacgga
     1501 ttcgatgccg taagcgatgt aatagcatcc gctttgcttc atcaaaacaa taagatcttc
     1561 cgatatgtga tcggcgcgga cgccgttcgg gcaggcccag tgtatcttga tgtttttttc
     1621 aatcaggaga cggcaaaact ttttaacgtt atcggcgttc agcgtgaaat tatcatcttc
     1681 gaaatgaact tcgctgatgt tgaaattttt catcagataa tcgatttcgg caacgacatt
     1741 ttccggtgtt ctgaatctga tggttctgtc gcagaatcgg ggagcggcgc aaaaggtgca
     1801 ctcaaaggga cacccccggc tggtgaccat aacacctacg ggaaagcccc ggacaaccgc
     1861 gccgtgcggg gcgcggggat attcccgcgg atcaatctgt tcccaatcgg ggaaaggcag
     1921 atcatcgaga ttgtccacca cggacgcttt gatgatctcc tgctgttcgc ttttgaggtt
     1981 ttccagcgaa tacacccccc gaatactctt gttgcaaaat ttactttcgg ccaattcaag
     2041 cagcggaatt tccccttcgc cgcaaacgac aaaatcggcc tgcgactcca acagcgtgcg
     2101 gtacggcaga aacgtaggat gctgaccgcc gatgacggtt gtgattttat tttttgcaag
     2161 ttcgcgggcc aacacgacca cttcttgata aaacgcggtg agacaggtaa tgcccaccgc
     2221 atcgggattg aatttcagaa tccggcgcat cagccgcgac gggctgagat tctctttcag
     2281 accatcaata atttttgtct cgatgccgga ctttttgaga tacgacgaga gataccccag
     2341 gccgatcggc ggtgttatga tatggctttt atagtttggt cggaccagtg ctattttcat
     2401 gtcgaacgct tcagatgtct cagtttatat aactgtataa tcagttttat tcccaacccg
     2461 ccaaaccgaa acagagcaat cgacgattgg ccggcattgc gcggattgaa agaaatttcc
     2521 tgccataaga ttttttctcc ttcccgggtc agcattacgg acagaagcac attgggaata
     2581 aagaaattgt cgggaattag cggcaggtac aggcgcaaac gttcagcctt cattaaacgg
     2641 aaaggggcgt tggcatcctt taacgaaaca ttgaacagaa gcgccagcac tcctttcagg
     2701 atccgcgaaa tgaaaaccct ggccgggcca tccgcccgtc ttgcgcgcca accgataata
     2761 aaatcgcagg tgtcccgttt ttcccagaac
//
LOCUS       DAONQZ010000146         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_37336_length_2763_cov_17.040620, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000146 DAONQZ010000000
VERSION     DAONQZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37336_length_2763_cov_17.040620"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /gene="recR"
                     /locus_tag="PLR13_12015"
     CDS             complement(<1..576)
                     /gene="recR"
                     /locus_tag="PLR13_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQC20110.1"
                     /translation="MKAYAQPIKKLIAELGKLPGIGEKTATRLANYILRSTEDDVIKL
                     AQSIVEVKKKIKLCRICLNLTEDDTCHICRDASRDQEVICVVEDPDALAAIEESGGFK
                     GTYHVLHGALAPLDGIGPDNLRLGELVQRINRGNIKEIILATNTNVQGESTALLLTEM
                     FKEKNIIITRIAMGMPMGGDLKYIDKMTISKS"
     gene            complement(590..898)
                     /locus_tag="PLR13_12020"
     CDS             complement(590..898)
                     /locus_tag="PLR13_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQC20111.1"
                     /translation="MQNFNTIMKQAKKMQERMASLQKELETKTVEAQAGGGMVSVVVN
                     GKYEIVSLKIEKEVVNPEDIEMLQDLIAAAVNEGVRKSQEMAAAEMGKITGGLNIPGF
                     "
     gene            complement(942..>1526)
                     /locus_tag="PLR13_12025"
     CDS             complement(942..>1526)
                     /locus_tag="PLR13_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20112.1"
                     /translation="STIETIEKKLQSGLSGNDEKTVPQSIAAQGSSGGMCSAGAANSF
                     SGECKDENHCPPDNAADLKGLRDNLKKFIKKESPILAAKIESAEILRCENNCLTIGFP
                     ANFIFLDEMKNVQKQRLEQIAGKFFQQNMTLVIETLSAENGGANGGRPRSKAGILNDI
                     KQEAMKQPLLRKIIDELPDARVIDVRVQTEKTKS"
     assembly_gap    1527..1626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1627..2510)
                     /gene="dnaX"
                     /locus_tag="PLR13_12030"
     CDS             complement(<1627..2510)
                     /gene="dnaX"
                     /locus_tag="PLR13_12030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQC20113.1"
                     /translation="MEYQVLARKFRPQTFEEVAGQAHVVKTLRNAIAQGRVAHAFLFS
                     GPRGVGKTSVARILSKALNCEKGPTPTPCNQCSNCIEITNNSSLDVHEIDGASNRGID
                     EIRELRENVKFAPASSRYKIYIIDEVHMLTDPAFNALLKTLEEPPAHVVFIFATTESH
                     KIPATILSRCQCYDFRRIPLKEIIANLELVASKEGINISPIALSWIAEAGDGSMRDSQ
                     SIFDQVISYAGLDVKDADVEESLGLAARQYLFRLSEAVLNQDAGSCLIILEEAYLAGI
                     DMKYFYQMLLKHFRNLLLV"
     gene            complement(2662..2759)
                     /gene="ffs"
                     /locus_tag="PLR13_12035"
     ncRNA           complement(2662..2759)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLR13_12035"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          591 a          713 c          616 g          743 t
ORIGIN      
        1 cgacttcgat atcgtcatct tatcaatata cttcagatcg ccgcccatcg gcatgcccat
       61 ggcgatgcgc gttatgatta tatttttttc tttgaacatt tcggtcaaaa gcaatgccgt
      121 ggactctccc tgaacattgg tgtttgttgc cagaataatt tccttgatat tacctctgtt
      181 aatgcgctga accagttcgc ccaaccttaa gttgtccggc ccgattccgt ccagcggcgc
      241 cagagccccg tgcagaacat gataagttcc cttaaaaccg ccgctttctt caatggccgc
      301 cagcgcgtcc ggatcctcca ccacacagat gacttcctga tctctggagg catcgcggca
      361 gatgtggcat gtatcatctt ccgtcagatt taagcaaatc cggcaaagtt tgatcttttt
      421 tttgacttcc acaatgctct gagccagctt gatcacatca tcttccgtgg aacgcaaaat
      481 ataattggcc agccgcgtgg ccgttttctc gccgatgccg ggaagtttgc ccagttcggc
      541 aatcagcttt ttaatcggtt gtgcatatgc tttcataaat gtaccttatt taaaatccgg
      601 ggatattaag ccctccggta attttcccca tctccgccgc ggccatttcc tgcgatttgc
      661 ggacgccctc gttcactgca gcggcaatca aatcctgaag catctcgata tcttcggggt
      721 tgacaacttc cttttcaatt ttcaacgaaa caatttcata tttgccgttg accacaacgc
      781 tgaccatgcc gccgccggcc tgcgcctcaa cggtttttgt ctccagttct ttctgaaggc
      841 tggccatacg ctcctgcatt tttttggcct gcttcatgat ggtgttaaaa ttctgcacgc
      901 ttcatcctcc tcatggttaa tgaatatttt tcattcggtt tttacgactt tgttttttct
      961 gtctgcactc tgacatcaat cacccgggca tcaggaagtt catcaatgat ttttcgcaaa
     1021 agcggctgtt tcatcgcttc ctgcttgata tcgttaagga taccggcttt gctgcgcggt
     1081 ctgccgccat ttgctccacc gttttctgca ctcagtgtct caataacaag cgtcatattt
     1141 tgttgaaaaa atttcccggc aatctgctcc agtcgctgct tctgaacgtt tttcatttca
     1201 tccaggaaaa taaaattcgc ggggaagccg attgtcagac agttgttttc gcaacggagg
     1261 atttcagcgg actcgatttt tgccgccaga atcggacttt ctttcttgat aaactttttc
     1321 agattgtcgc gcagtccttt caaatcagcc gcattgtcgg gcggacaatg attttcatcc
     1381 ttgcattctc cggaaaagct gttcgccgcc ccggcagagc acatcccgcc ggatgagccc
     1441 tgcgctgcaa tagattgcgg taccgtcttt tcatcgtttc ccgacaagcc gctctgtaat
     1501 tttttttcga tggtctcgat ggtggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnacca gcaacagatt ccggaagtgc ttcagaagca tctggtagaa atacttcata
     1681 tcgatacctg ccagataggc ttcctccaga atgatgaggc acgaaccggc atcctgattc
     1741 aagacagctt cggacaatct gaaaagatat tgccgtgcgg caagccccag gctctcctcg
     1801 acatcggcat ctttgacatc cagtcctgcg taggaaatca cctgatcgaa aatactctgc
     1861 gagtcacgca tgctgccatc gcccgcttcg gcaatccagg aaagagcaat cggactgatg
     1921 ttgatgcctt ccttggaagc gaccagttcc agattggcaa tgatttcttt cagcggtatg
     1981 cggcgaaaat cgtagcactg acaacgggaa agaatcgtgg ccggaatttt gtggctctca
     2041 gtcgtggcaa aaataaaaac gacatgcgcc ggaggctctt ccagtgtttt caggagcgcg
     2101 ttgaaggccg gatcggttag catatggact tcatcaatga tgtaaatttt atatcgggat
     2161 gacgccggcg cgaacttgac attttcccgc agttcgcgga tctcatcaat accgcggttg
     2221 gacgcgccat cgatttcatg aacatccagg gaagaattat tggttatttc gatacagtta
     2281 gagcattggt tgcagggcgt gggtgtcggt cctttctcgc agttgagcgc cttggacaga
     2341 atccgggcca ccgatgtttt cccgacaccg cgcggaccgc tgaaaagaaa agcatgggcc
     2401 acacgcccct gggcaatggc attgcgcagc gttttgacca catgcgcctg accggcaact
     2461 tcttcaaaag tttgcggcct aaattttcgg gctaatacct gatattccaa tgaaacctcc
     2521 gaaataattt ccccgcccct tgagaccgcg gtgcccttca gggtcgaggg gacttggggg
     2581 tggggtgcgc gatctcatta catcatcttg tccgttttcc ccgagggaac tttaatacag
     2641 cagaaaacag actggtattc tggtgaccgg gtttaccgca acacatggcg gacattgtta
     2701 ccgctgcttc cttccggacc tgacggggtt cacaagcctc cgttgcgcgg gacccggcca
     2761 tat
//
LOCUS       DAONQZ010000147         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_37999_length_2728_cov_23.790498, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000147 DAONQZ010000000
VERSION     DAONQZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37999_length_2728_cov_23.790498"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>2728)
                     /locus_tag="PLR13_12040"
     CDS             complement(157..>2728)
                     /locus_tag="PLR13_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQC20114.1"
                     /translation="WANADQYYIKTSEYLKDYSFKLPGGKKAHFKLVEATQEKDNIKA
                     AAGKERKFKLHEDEPIHIEGKEVFIRFVYLPEKEKQDALNKKTVEFINGEATKRLKDF
                     PELFAAAPTAKNANRTLWEKHLTSYTARFNFDFFIHKDLGGFLRRELDFYIKNEVLFV
                     DDLDAQDERQLKSAVAKIKVIKYIALKIIAFLAQLEDFQKKLWLKKKFVVNCGYCVTL
                     DRLPEELYPVIAQNESQREEWVKLFAIDEIEGDLSKPGYSKPLTVEFLKANPYLVLDT
                     KHFDAKFKEKLLASFDNLDEATDGIIINSENFQALNLLLEKYQKKVNCVYIDPPYNTG
                     DSKILYKNEFDSSAWLSLMQNRLDISEYIIAEDFVYFIAIDDYEMVPLCELVDTHFDF
                     RREMIIINHHPQGGKAVTIANTHEYMLALVDSGSDRKLVGRTISEDTEERPFKRSGSA
                     ISNFRKGRPNSFYAILVDPNTKNIVGIENPPDKDAVYSTEDTHEGYRRIYPIGNNGVE
                     RVWRNAYETGKKLFETGKLKSSEHYTIYQLIEHGEKKTALFSNWTDPRYNAGTNGANL
                     LNDIIGIQNSFPYPKSIYTVEDALFTYQDSENSLILDYFGGSGTTGHATINLNREDDG
                     NRKYILVEMGVHFDSVLKPRIQKVIYSKDWKDGKPVSRDGISHMFKYMYLESYDDTLD
                     NLKAVRTKDQETLLEKMSAGAREDYMLSYMLNVETKASDSLLNIDRFSDPFNYTLKIR
                     RDNELKDEKIDLVETFNYLIGLYVEQTETIRGFKVVRGKLRTGEKTLVIWRNTQEKSN
                     EALDEFFQKQKYNTLDFEFDLIFVNGDNNLENLKVGDEKWKVRLIEEEFKKRMFAEDV
                     "
BASE COUNT          713 a          605 c          512 g          898 t
ORIGIN      
        1 gatcctcatt cagcgccacc ggattgacta catcaagcaa tcccagcata aagaaacgtg
       61 agcatccctc gtgtccgata atcacacggc ccctggcatc gaatatagtt ccgtcgcccg
      121 cctcatcaac gtagtaatat ctgacagtgg aatttgtcat acatcctctg caaacatgcg
      181 ttttttgaac tcttcttcga tgaggcgcac cttccatttt tcatcgccga ctttgagatt
      241 ttccagattg ttgtcgccgt tgacaaaaat caaatcgaat tcaaaatcca gcgtgttgta
      301 tttctgtttc tggaagaatt catccagagc ttcattggat ttttcctgag tattacgcca
      361 gattaccagc gtcttttcgc ccgtgcgcag cttcccgcgc acaaccttga aaccgcggat
      421 ggtttccgtc tgttctacat aaaggccgat tagataattg aacgtctcca ccagatcaat
      481 tttttcgtct ttgagttcgt tgtcccggcg gattttcaag gtgtagttaa atggatcgct
      541 aaagcggtca atgtttaaga gcgagtcgct ggccttcgtt tcgacattca gcatatacga
      601 aagcatgtaa tcttcgcggg cgccggcgct cattttttcc agaagcgttt cctggtcttt
      661 tgtccggacg gcttttaaat tatccagggt gtcatcataa gattccagat acatgtattt
      721 aaacatgtgg ctgataccat cgcgcgacac cggcttgccg tctttccaat ccttggaata
      781 aattaccttc tgaatgcggg gtttaagaac agagtcaaaa tgaacgccca tttcgacaag
      841 gatatatttc cgattgccat catcttcacg atttagatta attgttgcgt gcccggttgt
      901 tccagaacca ccaaaataat caagaattag actattttcc gagtcttgat atgtaaataa
      961 tgcatcttca accgtataaa ttgattttgg atacggaaag ctattttgaa taccaataat
     1021 atcatttaat aaatttgcac catttgtacc agcgttatat cttggatcag tccaattact
     1081 aaacaatgct gttttctttt caccgtgttc tattagttgg taaatagtat aatgttcact
     1141 gctttttaat tttcctgttt caaacaactt tttgccggtt tcataggcat ttcgccacac
     1201 acgttccaca ccattattgc ctattggata aattcttcta tatccttcat gtgtatcttc
     1261 tgttgagtac acagcatctt tatctggtgg attctcaatc ccgacaatat ttttagtatt
     1321 tggatcaaca agtattgcat aaaaactatt tgggcgacct tttctaaaat tactaattgc
     1381 cgaaccactt cgtttaaatg gtcgctcttc agtatcctct gaaattgttc ttccaactaa
     1441 tttcctgtct gatcctgaat ctaccaaagc aagcatatat tcatgtgtat tagcaatagt
     1501 tacagctttg ccaccttgag gatggtggtt tataattatc atttctcttc tgaaatcaaa
     1561 gtgtgtatca accagttcac ataaaggcac catttcataa tcatcaatag caataaaata
     1621 tacaaaatct tctgcaatga tatattcact tatatcgaga cgattttgca tcagagatag
     1681 ccaagcagag gaatcaaatt catttttata caatatcttc gaatctcctg tattgtaagg
     1741 tggatctatg tagacgcaat ttaccttttt ttgatacttt tctaataata aattcagtgc
     1801 ctgaaaattt tcggaattga taatgatacc atcggtggcc tcatccaggt tgtcaaagct
     1861 ggccagcagt ttttctttaa atttcgcgtc aaagtgtttc gtgtccaaaa ccaaataggg
     1921 attggctttg agaaattcta ccgtcagcgg tttcgagtat ccgggcttgg ataaatcgcc
     1981 ttcaatctca tcaatggcaa aaagctttac ccattcctcg cgctggcttt cgttctgggc
     2041 aatcaccggg taaagttctt ccggcaagcg gtcgagtgtt acgcagtagc cgcagttcac
     2101 cacaaatttc tttttcagcc agagcttctt ttgaaaatct tccagttgcg ccagaaaggc
     2161 gataatcttc agggcaatgt atttgataac cttgatcttg gccacggcgc ttttgagctg
     2221 tctttcgtcc tgcgcatcca gatcatccac aaaaagtact tcgttcttta tgtaaaaatc
     2281 tagctcgcgg cgtaaaaatc cgcccaaatc cttatgaata aaaaaatcga aattgaacct
     2341 ggcggtatag gatgtcaggt gtttttccca gagcgtgcga ttggcatttt tggcagtcgg
     2401 cgcggcggca aagagttccg gaaaatcttt tagtctcttt gtagcttcgc cgttgatgaa
     2461 ttcaacggtt tttttgttga gcgcgtcctg tttttctttt tccggcaaat agacaaaccg
     2521 gataaagact tcttttcctt caatatgaat cggctcgtct tcatgaagtt tgaatttacg
     2581 ttccttgccg gctgcagctt taatattgtc tttttcctgc gtggcttcca ccagtttgaa
     2641 atgcgccttc ttaccgccgg gaagcttgaa tgaataatcc ttcaggtatt cgcttgtttt
     2701 aatataatat tgatcagcat tggcccaa
//
LOCUS       DAONQZ010000148         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_39173_length_2665_cov_14.368966, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000148 DAONQZ010000000
VERSION     DAONQZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39173_length_2665_cov_14.368966"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..748)
                     /gene="pgsA"
                     /locus_tag="PLR13_12045"
     CDS             complement(191..748)
                     /gene="pgsA"
                     /locus_tag="PLR13_12045"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQC20115.1"
                     /translation="MISRRDTFNLPNTITLLRIGVVPFLFILLSDPGKSWSLIIAGLF
                     VVASITDFFDGYIARKYQMITTMGKFLDPIADKIIVNSAMILLIPIGRIPAWIVAITI
                     IRDLMVDVIRSIASSEGIYIQASRLGKQKTVAQIIAVTALIIHYPFLGFNTHVVGTVV
                     LYVAFIFTIYSGIDYLVKLFQSIEQ"
     gene            complement(786..1433)
                     /gene="fsa"
                     /locus_tag="PLR13_12050"
     CDS             complement(786..1433)
                     /gene="fsa"
                     /locus_tag="PLR13_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQC20116.1"
                     /translation="MKFFIDTANIDEIKQGIALGMVDGVTTNPSLIAKEKKGFDVVVK
                     EILKNVQGPVSLEVVSHEAKEMFAEGKKLARLGSNVVIKVPLSTEGLKATKMFADAGI
                     DVNQTLIFSPLQALMAAKAGARYVSPFVGRLDDIAHDGMELVEQIITIFENYCYETEV
                     IVASIRHPRHVLDAALMGADIATIPFKVIAQLVQHPLTEKGIETFLNDWKKVPKK"
     gene            complement(1449..1700)
                     /locus_tag="PLR13_12055"
     CDS             complement(1449..1700)
                     /locus_tag="PLR13_12055"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HQC20117.1"
                     /translation="MIRLLISIAVIYILYKILKTFRQMKAEKSESRTRSSAGEDLVED
                     PVCHTYVPLSQAYKKDIDGKSYYFCSKDCSDKFPLEKNT"
     gene            complement(1720..2217)
                     /gene="folK"
                     /locus_tag="PLR13_12060"
     CDS             complement(1720..2217)
                     /gene="folK"
                     /locus_tag="PLR13_12060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQC20118.1"
                     /translation="MDGIICYIGIGSNLGDALNNCRKAVGLISRTGGISLERVSSFYK
                     TEPVGIQPQDDFVNAVVEIKTTLGAADLLQKLQDLENTMGRTRTVKGGPRTIDLDLLF
                     YGQQVINAKGLVVPHPEAHKRRFVLEPLCDIASYFIHPSFGVSIRGLQNRLTDDKKVE
                     KLMEK"
     gene            complement(2234..>2665)
                     /gene="dapB"
                     /locus_tag="PLR13_12065"
     CDS             complement(2234..>2665)
                     /gene="dapB"
                     /locus_tag="PLR13_12065"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQC20119.1"
                     /translation="GVNVMLKVLEYCAGILKDDYDVEIIEAHHHLKKDAPSGTAMQMA
                     RVIAEKLQRDPEECFVYSRHGLIGERTKKEIGIQTVRAGDIVGDHTVIFGGVGERLEF
                     VHRAHSRDNFAKGAIRAAQWIVSQKNGLYDMQDVLGLRTGK"
BASE COUNT          655 a          733 c          593 g          684 t
ORIGIN      
        1 accgtcgacg tctaccttgg caaggtagca ctctaccgct gagttacgcc cgctcactta
       61 aaacggcgtt gactataaca aatcgctgct tcttgtcaat aaaaattaat attttattca
      121 taaaaaaatg cacgacgctt cttccgaggc gatggtgaag acggcggttg aaaaatccgg
      181 tgggcaatcc ttattgttca atgctttgaa acaatttcac gagataatca atcccggaat
      241 aaatcgtgaa aataaacgca acataaagga cgacggtccc gacaacgtgg gtgttgaaac
      301 ccaaaaaagg ataatgaatg atcaacgccg tcacggcgat gatctgcgcg acggttttct
      361 gctttcccag ccggctggcc tgaatataaa ttccttccga agacgcaata ctcctgatca
      421 cgtccaccat cagatcgcgg atgatcgtga tggcaacgat ccatgccggt atgcgtccga
      481 tgggaatcag caggatcatc gccgaattga cgatgatttt gtcggcaatg gggtccagaa
      541 attttcccat ggttgtgatc atctggtatt ttcgggcgat ataaccgtcg aaaaaatccg
      601 taatggaggc caccacaaac aatcccgcga tgatcagact ccaggatttc cccggatccg
      661 aaagcagaat aaaaagaaaa ggcacgacgc cgatcctcag cagggtgatt gtgttgggca
      721 gattgaatgt atcccgtctc gatatcattg gaatgccttg tccataaatc gcatcaaccg
      781 tttgcttatt tctttggaac cttcttccaa tcgtttaaaa acgtctcgat gcctttctcc
      841 gtgagcggat gctgaaccag ttgggcgata accttaaagg gaatcgtggc gatatccgcg
      901 cccatcaatg cggcatccag aacatgccgg ggatggcgga tactggccac aatcacttcc
      961 gtctcatagc aataattttc aaagatggtg atgatctgtt ccaccagttc catgccgtca
     1021 tgcgcgatat catccagcct gccgacaaag ggactgacat accgggcccc ggccttggcc
     1081 gccatcagcg cctgcagggg agaaaagatc aacgtctgat tgacgtcgat accggcatcg
     1141 gcaaacattt ttgtcgcctt caagccttcc gtcgacagag gaaccttgat cacaacgttg
     1201 ctgccgaggc gcgccagttt ttttccttcg gcaaacatct ccttcgcctc atgactgacc
     1261 acttccaggc tgaccggacc ctggacattt ttaagaattt ccttcacaac gacatcaaac
     1321 cctttcttct ccttggcgat cagggacgga ttggtcgtca cgccgtcaac catcccgagg
     1381 gcaatcccct gtttgatttc atcgatattg gcggtgtcga taaaaaactt cataaaaagc
     1441 tccttgcatt atgtattttt ttccaatgga aatttatcgc tgcaatcttt gctgcaaaaa
     1501 taataggact tgccgtcgat gtcctttttg taagcctgac tcaacggaac atatgtatgg
     1561 cacaccggat cttccaccag atcttctccg gccgacgacc gggtcctgct ctcgcttttt
     1621 tccgctttca tttgccggaa tgtttttaaa attttataca gtatgtaaat gaccgctatg
     1681 gatatcagta atctgatcaa tataaaacca ccttcatcgt tatttttcca tcaatttttc
     1741 cactttttta tcatccgtca gccgattttg caatccccgg atggacacgc cgaatgacgg
     1801 gtgaatgaaa tacgaggcga tgtcgcaaag cggctccaga acaaaccgtc gtttgtgggc
     1861 ttcgggatgc ggaactacca gacccttcgc attgatcacc tgctgcccgt aaaaaagaag
     1921 atcgagatcg attgtccgcg gtccgccttt caccgtccgg gttcggccca tagtgttttc
     1981 caggtcttgc agcttctgca acagatcggc agccccgagc gttgtcttaa tttcaacaac
     2041 cgcattgaca aaatcatcct ggggttgaat cccaaccggt tccgtcttat aaaaggatga
     2101 caccctttcc aggctgatcc ccccggttcg cgaaatcagt ccgacagctt tccggcaatt
     2161 gttcaaagcg tcgccgagat tggacccgat cccgatatag cagatgatac cgtccaataa
     2221 aagcccgtcc ttattatttc ccggtcctta atcccagaac gtcctgcatg tcatacagac
     2281 cgtttttctg gctgacaatc cactgcgccg cccggatcgc ccctttggcg aagttgtccc
     2341 ggctgtgcgc ccggtggacg aattccagcc tttccccgac accgccgaaa atcaccgtat
     2401 gatctcccac gatatcgccg gcgcgaacgg tctgaatgcc gatttccttt ttcgtcctct
     2461 cgccgatcag gccgtgccgg gaataaacaa aacattcctc cgggtcccgc tgaagttttt
     2521 ccgcgatcac cctggccatc tgcatggccg tcccgctggg ggcgtccttt ttcaggtgat
     2581 gatgcgcctc gataatctcg acatcgtaat cgtccttcag aatgcccgcg caatattcga
     2641 gaaccttcag cattacgttg acgcc
//
LOCUS       DAONQZ010000149         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_40124_length_2617_cov_10.759953, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000149 DAONQZ010000000
VERSION     DAONQZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40124_length_2617_cov_10.759953"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1447)
                     /locus_tag="PLR13_12070"
     CDS             complement(167..1447)
                     /locus_tag="PLR13_12070"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HQC20120.1"
                     /translation="MAKTNNSAENDRKIKLKTLPHAKDLKWPMLWWFTMGSIYHALPW
                     KKTAWTCAAFPIELMWIYDIFPDHPENMATVAAARKQSQRLIEHAEGMGYSRDLCSYC
                     KTSIAAYDTKIKKTLGGISKPDIVVCTNAICDTHWKWFQIMADKMNVPFFMFDIPKWV
                     SGTDEKTLQGNIDYVVEQFYDLIVFIEKHTGKKMDEKRLYKVLDKSTELSDLWREIFE
                     HRKAVPSPYSGAETSASFFPLVVLPGVQVGINFFKKILADIKNLTAKGEGTMPKDKEK
                     YRVMWEGIPFWYRMRFMYDLAQYGAVITFEPYTYSFAPEKKKCNTMEETLRDVAKLIM
                     DTPYAYNLERRIEHFEKWIDEYKIDGVILHENMSCRPSSAGMVDLAEAIMRDKGIPVL
                     ILQCDMNDPRAYSEGQIKTRVEGFIELMEANKGK"
     gene            complement(1440..2504)
                     /locus_tag="PLR13_12075"
     CDS             complement(1440..2504)
                     /locus_tag="PLR13_12075"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HQC20121.1"
                     /translation="MKIIKAQKLAYFDTSHEMPLEIIMAAGFTPYKILGDVHQSNDPA
                     DKHLSNFFCGAAKSWLTEALARSKEWAGIIFAQGCNNTNRHYDIWKVHVQTPFLHWFG
                     APLLDSDAAKKYYRVELQRLISHLENQFNIKITPEKIREAIIKSNEIKKKLQRLSALR
                     AQKDIPNRDYLAAMIKCLTNPVDEVSAWLDSEIAAWNSKPSFPSGKKQFLLTGSDITY
                     VEWMDTLEDCGIRIVRDDLSVGERFFATLIPDKSDPLDALIEYYMNIPKPATRPTIQK
                     RLDYLYKALGETKVDGVLSQNVKFCEPFAYDSVPVNNALKDKGYKVIHLERDFSPAAD
                     QQLANRLMAFVEMMEVGNNG"
BASE COUNT          612 a          632 c          604 g          769 t
ORIGIN      
        1 aacctcttat tcctttggtt ggatattata agtgtttatt cagtgacaat ttttccccta
       61 gtttggtcga gtcttttgga ctcgaccttt tatcagttca cgatatttac gacagccaag
      121 tcaaaaagac ttggctgaac tgcagagcaa aagacaacgg ttctatttac ttgcctttat
      181 tcgcctccat gagttcaata aacccctcca ccctcgtttt aatttgccct tcggagtagg
      241 cccggggatc gttcatgtca cattggagga tcagcacggg aatgcccttg tccctcatga
      301 tggcctccgc cagatcgacc atgccggcgc tggatggcct gcacgacatg ttttcgtgca
      361 gaatgacgcc gtcaattttg tattcgtcaa tccatttttc aaaatgctct atacgtcttt
      421 cgaggttgta ggcgtagggc gtgtccatga tgagtttggc gacatcgcgc aatgtctctt
      481 ccatggtgtt acactttttc ttttcgggag cgaacgagta ggtgtaaggc tcgaaggtaa
      541 tgaccgcgcc gtattgcgcg aggtcataca tgaacctcat ccggtaccag aagggaatgc
      601 cttcccacat cacacggtat ttttctttgt ctttgggcat ggttccctcg ccctttgcgg
      661 tgaggttttt gatatcggca agaatttttt tgaaaaaatt tatgccgacc tgaacacccg
      721 gcagaaccac caggggaaaa aatgacgccg atgtctccgc tccgctgtag ggcgacggca
      781 ccgcctttct atgttcaaaa atttcccgcc acaggtcgga cagttccgtc gatttatcca
      841 aaactttgta cagtcttttc tcatccattt ttttaccggt gtgcttttca ataaatacaa
      901 tcaggtcgta aaactgctct accacgtaat caatattacc ctggagggtt ttctcatccg
      961 taccgctcac ccatttggga atatcaaaca taaaaaacgg gacgttcatt ttatcggcca
     1021 taatctgaaa ccacttccag tgggtatcgc agatggcatt ggtgcacacc acgatatcgg
     1081 gcttgctgat tccgccgagc gtctttttga ttttggtatc gtaggcggca atgcttgtct
     1141 tgcagtagga acacaggtca cgtgaataac ccattccctc ggcatgctca atcagacgct
     1201 gggactgctt gcgggcggcc gccacggttg ccatattttc agggtgatcg ggaaaaatat
     1261 cgtaaatcca catgagctca atgggaaagg cggcacatgt ccaggccgtc tttttccaag
     1321 gcaaagcatg gtatattgac cccatggtaa accaccacag catcggccac ttcaggtctt
     1381 tcgcgtgagg cagggttttg agttttatct ttctgtcatt ctcagcagaa ttgtttgttt
     1441 tagccattgt tccctacctc catcatttcc acaaaggcca taagccggtt ggcgagttgc
     1501 tggtcggccg caggagaaaa gtctcgttcc aggtggatca ccttgtagcc cttgtctttc
     1561 agggcattat tgacgggcac ggaatcatag gcaaaaggct cgcagaattt cacattttgc
     1621 gaaagaacac cgtcaacctt tgtctcaccg agagctttat aaagatagtc caaccgtttc
     1681 tgaatggtcg gccttgtcgc gggcttgggg atgttcatgt aatattcaat aagggcatcc
     1741 agcggatcgc tcttgtcggg aatcagtgtc gcgaaaaatc tttctcctac ggaaaggtcg
     1801 tcacgaacaa tccgtattcc gcaatcttcc agcgtgtcca tccattccac atacgtgatg
     1861 tcgctgccgg ttaataaaaa ttgttttttg ccggacggaa aagagggctt tgaattccag
     1921 gccgctatct ctgagtcaag ccaagccgaa acttcatcca cgggattcgt aagacatttt
     1981 atcatcgcgg caagataatc gcggttggga atgtccttct gtgctctgag cgcagagaga
     2041 cgttgcagct ttttcttgat ttcgttggat ttgataatcg cctctcttat tttttcaggc
     2101 gtaattttta tattaaattg attttccaga tggctaatca gtctttgcag ttcaacccgg
     2161 taatattttt tcgcggcgtc actgtccagc agaggagcgc cgaaccagtg caggaaaggc
     2221 gtttggacat gaaccttcca aatgtcataa tgccggttgg tattgttgca cccctgcgcg
     2281 aaaataatgc cggcccattc tttggagcgg gccagagctt ccgtgagcca gctctttgcg
     2341 gcgccacaga aaaagttgga taaatgttta tccgctgggt cattggattg gtggacatct
     2401 cccagaattt tatacggggt aaatccggcc gccatgataa tttccagcgg catctcatgc
     2461 gatgtgtcaa aataggcaag tttttgcgct ttgataatct tcactccaaa aagttttctt
     2521 tattgctagg ccaactctta agaaatatct cgtcgttatg aattctcccc tgtttacatt
     2581 taggttattc agggtacttg gttctcggaa tatcccc
//
LOCUS       DAONQZ010000150         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_40227_length_2612_cov_8.300743, whole genome shotgun sequence.
ACCESSION   DAONQZ010000150 DAONQZ010000000
VERSION     DAONQZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40227_length_2612_cov_8.300743"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLR13_12080"
     CDS             <1..690
                     /locus_tag="PLR13_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQC20122.1"
                     /translation="NFSNQAKVFASVAHKSRFPMLEELYSSKGGNVNLETEKSWNYTL
                     GASSFLTRYAKAGLSAFYYDVSDMISKDGMGWIGTNYNVGKVQLYGFEFNAEIYPIDG
                     LTLRLDYTYEEGRNRSEGRVTNDVTYIPRHKLDAGMQYVIPMLKTRLDFNMIHVSSTY
                     SQLPTPSSPSNPVLKASDYTLFDAKITQPIWKYFEVYVACRNIFDKNYEPEVGYPAPG
                     RSFWTGILARY"
     gene            731..1165
                     /gene="exbB"
                     /locus_tag="PLR13_12085"
     CDS             731..1165
                     /gene="exbB"
                     /locus_tag="PLR13_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HQC20123.1"
                     /translation="MDWLIFIVDYGIIGFLILLSVIAVGIGIERFFFFRTIRLDAFTD
                     YKSLELELTRKIHLIATIGSNAPYIGLLGTVLGIMLTFYMIGSDGFMDSGKIMTGLAL
                     ALKATAVGLLVAIPSVSLYNFVLRRVKVLLMTWEIKHGRKDL"
     gene            1146..1526
                     /locus_tag="PLR13_12090"
     CDS             1146..1526
                     /locus_tag="PLR13_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQC20124.1"
                     /translation="MEEKTFDYINVVPLVDVMLVLLVIVLTTSSFIATGMIPLNLPKA
                     SHTTDGAFKTQTIEIDQDGQTYLNGRRVSPTELKTGLAAMNRNVPVLIRADRSVRLQS
                     FVSILDIIKNLEFNHVSLQTEEGI"
     gene            1523..2251
                     /locus_tag="PLR13_12095"
     CDS             1523..2251
                     /locus_tag="PLR13_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQC20125.1"
                     /translation="MNNQARAFRISFLLHGAIIGLMFLSAPLPGPLKKTIPLDFSLLD
                     PYSKQRIVEPAASFVQTAPAQPPVRERKAQSPPARLESETPRTPEISPAAELTATEAQ
                     LVSTALAEESGDTGEPTAHLPADAQSGNEAPIKVTNTGDAAGAMESAASKYLNEHFDY
                     IRKKILGNINYPDAAKRMGWQGKVVLSFVITSDGSVRSLKIMQSSGFKILDDKAIETI
                     RQAAPFPRPPVEARLMIPVVYHLN"
     gene            2320..>2612
                     /locus_tag="PLR13_12100"
     CDS             2320..>2612
                     /locus_tag="PLR13_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HQC20126.1"
                     /translation="METNKNLDFENCLNEAKAFHGDACAGTILGTRMAILGLNLIGIA
                     DPKGADRKDLIVFVEMDRCASDAILAVTGCHPGKRSMKIFDYGKMAATFVNLKT"
BASE COUNT          687 a          674 c          646 g          605 t
ORIGIN      
        1 aatttttcca atcaggcaaa ggttttcgct tccgtagctc acaagagccg ctttcccatg
       61 cttgaagaac tttattccag caagggtggc aatgtaaatc tggaaacgga aaagagctgg
      121 aattacacac tgggagcctc gagttttttg accagatacg ccaaagccgg cctgtcggcg
      181 ttttactatg atgtatccga tatgatctcc aaagacggca tgggctggat cggcactaat
      241 tacaatgtcg gtaaagtcca gctgtacggc ttcgaattca acgctgaaat ctacccgatc
      301 gacggactga cgctcaggct ggattatacc tatgaggagg gcagaaaccg cagtgagggg
      361 agagtgacca atgatgtcac atatatcccc aggcacaagc tggatgccgg gatgcaatat
      421 gtaatcccta tgctcaaaac cagacttgat tttaatatga tccatgtatc atcgacatat
      481 tctcagttgc cgacaccgtc gagcccgtca aacccggttc ttaaagcgag tgattacact
      541 ctgtttgatg cgaaaatcac acagccgata tggaaatatt ttgaagttta cgtggcctgc
      601 agaaacatct ttgacaaaaa ctacgagccg gaagtcggct accccgcccc ggggcgaagc
      661 ttctggaccg gaattctggc aagatactga taccggcata atatatcttt gatgagggtc
      721 ggaatctttc atggactggt taatatttat agtcgattac ggaattatcg gatttttgat
      781 tctgcttagc gttatagctg tcggcatcgg catcgaacgg ttctttttct tcagaaccat
      841 aagactggat gccttcaccg actataaaag ccttgagctg gagctcacgc gcaaaatcca
      901 tctcattgcc accatcggaa gcaatgcgcc gtatatcggc ctgttgggaa cggttctggg
      961 catcatgctc acgttttaca tgatcggcag tgacggcttc atggactcgg gcaaaatcat
     1021 gaccggcctt gccctggccc ttaaagccac agccgtaggc ctgctggtgg ccattccgtc
     1081 cgtctctttg tataattttg ttttgcgaag agtaaaggtc ctgctcatga cctgggagat
     1141 caaacatgga agaaaagacc tttgattata tcaacgtcgt tcctcttgtg gatgtgatgc
     1201 tggtgctttt ggtcattgtg ctgaccacat cctccttcat agcgacagga atgatccccc
     1261 tgaatttgcc gaaagccagt cacactacgg acggcgcctt caagacccaa actattgaaa
     1321 tagaccagga cggccaaacc tatctcaacg gccggcgtgt ttctcctacg gaattgaaaa
     1381 ccggtcttgc cgccatgaac agaaacgttc ctgttttaat ccgggcggac cgcagtgtca
     1441 gactacaaag ctttgtcagc attttggata tcatcaaaaa tctcgaattc aatcacgtca
     1501 gccttcagac ggaggaaggg atatgaataa tcaggccagg gcctttcgga tatccttcct
     1561 gctccatggt gccattatcg gattaatgtt tttaagcgcc cccctgccgg ggccgttaaa
     1621 aaaaacgatt cctctggatt tcagcctgct tgatccgtac agcaaacaaa ggatagtcga
     1681 accggccgcg tcctttgtac agaccgctcc ggcgcagcca cccgtgcgtg aaagaaaggc
     1741 acaaagcccg ccggcgcggc tggaaagtga aactccacgc acccctgaaa tctcacccgc
     1801 agcagagctt accgcaactg aagcccagtt ggtcagcacc gcgcttgctg aagagtctgg
     1861 cgataccggt gagccgacgg cacacctgcc ggcagacgcg caaagcggaa atgaagcccc
     1921 gataaaggtc acaaacacag gtgacgcggc cggcgccatg gaatcagccg cctcaaagta
     1981 tttaaacgaa cactttgatt acattcgtaa aaagatactg ggaaatatca actatcccga
     2041 tgctgcaaaa aggatgggct ggcagggaaa agttgttctt tcctttgtga tcacctctga
     2101 cggatcggtc agatcattga aaattatgca aagttccggc tttaaaatac tcgacgacaa
     2161 agccatcgaa actatccggc aggccgcgcc cttcccccgc cccccggtgg aagccaggct
     2221 gatgattccc gttgtctatc atctgaattg agcattggaa aaattcacgg ggagtgtctt
     2281 cgccgacagg cacccccggt cattatggag gaatgaatca tggagacaaa caaaaacctg
     2341 gattttgaaa actgtctcaa cgaggccaag gcgtttcacg gagacgcctg cgcggggacg
     2401 atcctgggca cccggatggc cattttgggg ctcaacctga tcggcattgc cgatccgaaa
     2461 ggcgccgaca ggaaggattt gatcgtcttt gtcgaaatgg accggtgcgc cagcgacgcg
     2521 atactggcgg tgaccggctg ccaccccggc aaacgcagca tgaaaatttt cgattacggg
     2581 aaaatggcgg caaccttcgt caacctcaaa ac
//
LOCUS       DAONQZ010000151         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_41469_length_2554_cov_17.494998, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000151 DAONQZ010000000
VERSION     DAONQZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41469_length_2554_cov_17.494998"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="PLR13_12105"
     CDS             <1..1539
                     /locus_tag="PLR13_12105"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HQC20127.1"
                     /translation="YPHIHHLIPSRRDLGIGGCWNEAIYSPYCGLYVVQLDSDDLYSS
                     PHTLQKVVNKLRRGRYAMVVGSYTIVDEHLKKIPPGLIAHKEWTQTNGHNNLLRVNGM
                     GAPRAFDQSALCRTGFPNVSYGEDYAVALRITREYKIGRIYENLYWCRRWKDNTDAGL
                     DAEKKNRNDFYKDQLRTLEIEARKSANKGDACVNSRRVLAEFSGGKALALPFLCQHLY
                     DAQKKTWPKLAEACRNLASVHKRKLPGAYDVYLQYNPARAISSGASVDAQSIRQRPCF
                     LCQENLPVEQKGILYRSQYLILCNPAPIFEKHFTIVSLQHTLQEITSSINGFLQLSAD
                     MSGYAVFYNGPACGASAPDHLHFQAVPKKFLPFLREIKKLPVCDTSSLRYASGETFDR
                     SVVLLESGDRDALSMQFETLLKALQSIMQTKVEPMVNVVCDHAGHRWRLIVFLRQKHR
                     PDAYFEKGEKRIFISPGAVDMAGVVITPLLDNYNRLDYNAVREIYRDVSLSENTTDAV
                     IRKL"
     gene            1554..2108
                     /locus_tag="PLR13_12110"
     CDS             1554..2108
                     /locus_tag="PLR13_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQC20128.1"
                     /translation="MFFAEEAIVDSAMSFSEAIEGTTAPDEIILSLSLVDVFYYSFDG
                     RKHQGQMLISKELEDDAYDIFNVIEKIFFPVDKVIPITAYQWNDYRSMADNNTSSFNF
                     RVIEGTTKLSMHSLGKALDINPVQNPVIYPNGVVAPEGAKYQPDERGTFSADHPIVRE
                     FLKRGWHWGGNFNQPKDYHHFEKP"
     gene            complement(2172..>2554)
                     /locus_tag="PLR13_12115"
     CDS             complement(2172..>2554)
                     /locus_tag="PLR13_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HQC20129.1"
                     /translation="ATPHRKKIDFMSTHPELYPALKNKVQLAKIIDIEKMLSRKAFPV
                     MNKEKLGTRIKKIQSGDVIAFASNQQGLDVAHVGFALWQGNSLRLLHASRQEGKVVIS
                     KKTLLAYLTSNKKFTGIIVARSVF"
BASE COUNT          654 a          573 c          639 g          688 t
ORIGIN      
        1 tatccccaca ttcatcatct gattccttcg cggcgcgatc tcggaatcgg cggttgctgg
       61 aatgaagcca tttattcgcc gtattgcggc ctttatgtgg ttcaactgga ctcggatgac
      121 ctttacagct cgccgcatac gcttcagaaa gtcgttaata aattgagacg cggacgatac
      181 gcgatggtgg tcggttctta caccattgtt gacgaacatt tgaaaaagat tccgccggga
      241 ctgatcgcgc ataaagaatg gacgcagacg aacgggcata ataatttgct gcgcgtcaac
      301 ggcatgggag caccccgtgc gtttgaccag tctgctcttt gccggacggg tttccccaat
      361 gtcagctacg gggaggatta tgcggtggca ttgagaatca cccgtgaata taaaatcggc
      421 aggatctatg aaaatttgta ctggtgccga cgatggaagg ataacacgga tgccggtctt
      481 gacgcggaga aaaaaaaccg caacgatttt tataaggatc aactgcgaac actggaaata
      541 gaggccagaa agtcggccaa caaaggagac gcgtgtgtaa acagccggag agttttagct
      601 gaattttcgg ggggcaaggc tttagcattg ccttttcttt gtcaacatct ttatgatgcg
      661 cagaagaaaa cctggccgaa actcgctgaa gcttgtcgga atttggcgtc cgttcataaa
      721 agaaagcttc ccggcgccta tgacgtttat ctgcaatata atcccgcccg cgcgatcagc
      781 agcggcgcat ctgttgatgc ccagtccatc agacaaaggc cctgcttctt atgtcaggaa
      841 aatcttccgg tggaacagaa agggattttg tacagaagcc agtatctgat tctctgcaat
      901 cctgcgccga tctttgagaa gcattttaca atcgtatcct tgcaacatac gttgcaggaa
      961 attacgtcgt caataaacgg gtttttgcaa ttgagcgccg atatgtccgg ttacgctgtt
     1021 ttttacaacg gtccggcctg cggcgcttct gctcccgacc atttgcattt tcaggcggtt
     1081 cccaaaaaat ttcttccctt cctccgggag attaaaaaac tgcctgtttg cgatacatcg
     1141 tctttgcgat atgcgtcggg cgaaacgttc gatcgctccg ttgttttgct ggaatccggg
     1201 gatcgagacg ctttgagcat gcaattcgag acgttattaa aggcgctgca aagcatcatg
     1261 cagacgaaag tcgagccgat ggtcaacgtt gtttgtgatc atgcgggaca tcgctggcgt
     1321 ctgattgttt ttctgcgtca aaaacatcgg ccggatgctt attttgagaa gggggaaaag
     1381 cgaattttta tttcgccggg agccgtggac atggccggcg tcgtgataac gccactgctg
     1441 gataattata accgtctgga ttataatgct gtccgtgaaa tttaccgtga cgtatcgctc
     1501 tctgaaaaca caacggacgc ggtcattaga aaattataaa aagaaggttg cgaatgtttt
     1561 ttgccgaaga agcgattgtg gacagcgcga tgagtttttc cgaagcgatt gaagggacaa
     1621 ccgctcccga cgaaatcatt ctttcattgt cgcttgtgga tgtgttttat tattcttttg
     1681 acgggagaaa gcatcaggga cagatgctca tcagcaaaga attggaagac gatgcttatg
     1741 acatcttcaa cgtcatcgaa aaaattttct tcccggtaga caaggtgatt cccataacgg
     1801 cctatcaatg gaatgattac cggtccatgg cggacaacaa tacgtcctct ttcaatttcc
     1861 gggtgattga aggaaccact aaattatcca tgcattctct gggtaaggct ctggatatca
     1921 acccggtgca gaatccggtg atttatccca acggcgtggt ggcgccggaa ggagcgaaat
     1981 accagccgga tgaaagggga accttttcag cggatcaccc gattgttcgg gaatttttaa
     2041 agcgcggctg gcactggggt gggaatttta atcagccgaa agactatcat cacttcgaaa
     2101 aaccatgatg ccgacgtcaa atcaaaatac tatcagctcc gttctgatgt cttctttgac
     2161 ttgaaatcga tctagaatac tgatcgggcg acaataatgc cggtgaattt tttgttggat
     2221 gtcagataag ccaacagcgt ttttttggaa atcaccactt taccttcctg acgggaagca
     2281 tgaagcagac gtaaactgtt tccttgccac agagcaaaac cgacatgcgc cacatcaagc
     2341 ccttgctgat ttgaggcaaa ggcgatcaca tcgccgcttt ggattttttt tatccgcgtt
     2401 ccgagcttct ctttattcat tacaggaaaa gccttccggg aaagcatctt ttcgatatcg
     2461 attattttgg ccagttgtac tttgtttttc agagccggat ataattcggg atgcgtgctc
     2521 atgaaatcga tttttttacg gtgcggggtt gctt
//
LOCUS       DAONQZ010000152         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_41571_length_2549_cov_16.204090, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000152 DAONQZ010000000
VERSION     DAONQZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41571_length_2549_cov_16.204090"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..585)
                     /locus_tag="PLR13_12120"
     CDS             complement(211..585)
                     /locus_tag="PLR13_12120"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQC20130.1"
                     /translation="MVKRAHKNLDVWKESVSLATHIYKVTEKFPQNELYGLTSQMRRA
                     VISISSNIAEGAARNSAKEFSHFLNIAGGSLSELDTQIEIAHNLLFVTSEQKQDMNIM
                     IESISGKLAGLIKYVKKKSHEK"
     gene            complement(623..2521)
                     /locus_tag="PLR13_12125"
     CDS             complement(623..2521)
                     /locus_tag="PLR13_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQC20131.1"
                     /translation="MYAQFKNPNFYLMVITDVLLFTIALYLSYFFRFDFNFANINFEQ
                     IGDLLFWILPLKVFIFFTIGLYRGMWRYTSVKDFWLLIQACFIATVLIMVIILTISRF
                     SGYSRSIFFIDGIFTFVLTGGVRMAIRSFFAMFGNQAMNVSSSLLSKTTRVLIVGAGD
                     AGEKILREINDNYKLNYEVVGLIDDDEQKQGRTIHGVRVLGPVDRLPKILKRETIHQI
                     LIAVPSASGDRIRRIVETCQQCNLDYKILPGIGDLIDGRVSVKLLRDISYEDLLGRSP
                     VQLNVRDIRNYLDDKTILITGCGGSIGSELCRQVVKYQPHRLILLDSSESNLFNIQME
                     IINEHFFRYCEAILGHVQDEALMNNIFEKHKPEVVFHAAAYKHVPMMEKNPWQAVLNN
                     IMGSRVAMEMAIKHHVERFVLVSTDKAVRPTNVMGASKRVTELIMQCQQGNGTRFMAV
                     RFGNVIGSSGSVIPLFRRQIEQGGPVTVTHPEINRFFMTIPEAAQMILQAGTMGEGGE
                     IFILRMGTPIKIAEMARDLIRLSGKEPDVDIKIVFTGLREGEKLYEELITVGEDIMPT
                     SHKKVMVLQSNGFINGAKNSEEAKIYLYNKLDQLAGLAAQHDARGIKIKLKEIVPEYT
                     PQENESVF"
BASE COUNT          654 a          644 c          559 g          692 t
ORIGIN      
        1 attgctatta ttgttttcaa taagaaagtg aatagtgaat ggtgaacggt gaatggtccc
       61 ccacggcgcc tatagtaccc tttagagtat tcacccccgg gatagttcca ccgacgcttt
      121 gaacggtgtt tggcttgttc gccgcgggtg tcactacgat ttacgtttcg accttcgttg
      181 cactcgttct caactggtct tactaaaaag ttatttttca tgtgattttt tcttcacgta
      241 tttaattaat cccgccaatt tgccggatat ggattctatc ataatattca tatcttgctt
      301 ttgttcactg gtaacaaaaa gaagattatg ggctatttca atttgtgtat ccaattcgct
      361 cagtgagccg ccagctatgt tcagaaagtg agaaaattct ttcgccgaat ttcttgccgc
      421 tccttctgca atattacttg aaatagaaat aactgcacgg cgcatttgcg aagtcaatcc
      481 gtaaagttca ttttggggaa atttttctgt aactttgtaa atatgagtag caagtgaaac
      541 gctttctttc cagacatcca agttcttgtg tgctcttttt accattcact cttcaccctt
      601 cacttttcac tcttcactac attcaaaaga cactctcgtt ttcctgcggg gtatattcgg
      661 gtacaatctc cttgagtttt attttaatgc ctctggcatc atgttgcgcc gccagtccgg
      721 caagctgatc cagcttattg tagagatata ttttggcctc ttcggaattt ttcgcgccgt
      781 tgataaaccc gttggattga agaaccatga cttttttgtg gctggtaggc attatatcct
      841 ctccgaccgt gatgagctct tcataaagtt tttcgccttc gcgcaaaccg gtaaagacaa
      901 ttttaatatc cacatccggt tccttgccgg aaaggcggat caggtcgcgc gccatctcgg
      961 caatttttat gggcgttccc atgcgcagaa taaaaatctc gccgccttcg cccatcgtgc
     1021 ccgcctgcaa aatcatctgt gccgcttcgg gtatggtcat aaagaaacgg ttgatttccg
     1081 gatgcgtgac cgtgaccggt ccgccctgtt cgatctggcg gcggaacaac ggaataacgg
     1141 aaccggacga cccgatcaca ttgccgaacc gcacggccat aaaccgcgtg ccgttgccct
     1201 gctggcactg catgatcaat tccgtcacgc gcttgctggc gcccatgaca ttggtcggac
     1261 gcacggcctt gtcggtggaa acgagaacaa accgttccac atgatgtttg atggccatct
     1321 ccatcgccac ccggcttccc atgatgttat ttaataccgc ctgccacgga tttttctcca
     1381 tcatgggcac atgtttgtag gccgccgcgt ggaacacgac ttccggtttg tgtttttcaa
     1441 aaatattatt cattaacgcc tcatcctgca cgtgccccag gatggcttcg cagtatcgga
     1501 agaaatgttc gtttataatt tccatttgaa tattaaaaag attagattcg ctggaatcca
     1561 gcagaatcag acggtgcggc tggtatttaa cgacctgccg gcaaagctcc gaaccaatgg
     1621 agccgccgca cccggtgatc agaatggttt tgtcatcgag ataattccgg atatcccgca
     1681 cattaagctg caccggcgag cgtcccagaa gatcctcata gctgatatcc cggagcagct
     1741 tcacgctgac gcggccgtca atcaggtcgc cgatacccgg cagtatttta tagtccaaat
     1801 tacattgctg gcaggtttcc acaatccggc ggatccgatc gccgctggcg gaaggcaccg
     1861 caatgaggat ctgatgaatc gtttcccgct tcaaaatttt cggcaggcgg tcaaccggcc
     1921 cgaggactct gaccccgtga attgtccggc cctgtttctg ctcgtcgtca tcgatcaaac
     1981 cgaccacttc ataattcaat ttatagttat cattgatttc gcgtaaaatt ttttcgcccg
     2041 catcacctgc gccgacaatc aggactctgg tggttttgct gagcaatgac gagctgacgt
     2101 tcatggcctg atttccgaac atggcaaaaa aagaccggat ggccatgcgt acgccgccgg
     2161 ttaaaacaaa cgtaaaaata ccgtcaataa aaaaaatgct gcgggaatac ccggagaacc
     2221 ggctgatggt caaaataata accataatga gcacggtcgc tatgaaacag gcctgaatca
     2281 gaagccagaa atccttgacg ctggtgtagc gccacattcc ccggtaaagc ccgatggtga
     2341 aaaagataaa aaccttgagc ggaagaatcc agaaaagcag gtcgccgatc tgctcgaaat
     2401 taatattggc aaaattaaaa tcaaaccgga aaaaatacga cagataaagc gcaatggtaa
     2461 acagcaggac atccgtaatg accatcagat agaaattcgg atttttaaac tgagcataca
     2521 tccctattca ccatcaacca tcaatccct
//
LOCUS       DAONQZ010000153         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_42412_length_2509_cov_15.180929, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000153 DAONQZ010000000
VERSION     DAONQZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42412_length_2509_cov_15.180929"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..583)
                     /locus_tag="PLR13_12130"
     CDS             complement(80..583)
                     /locus_tag="PLR13_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQC20132.1"
                     /translation="MGKIIRSNTQKVYDKSPSSNGKRRIQVRQSGIHGKGVFAVHPID
                     AGETLLEYKGEIITWTKALARHPHDKSQPHHTFYFHLDDHHVIDAKYNGNSAKWINHS
                     CQPNLEAEQKGNRVFLKALRHIKAGEELSYDYSLVVDSRKTPRLKKEYACYCGAANCR
                     GTMLDCK"
     gene            complement(834..1166)
                     /locus_tag="PLR13_12135"
     CDS             complement(834..1166)
                     /locus_tag="PLR13_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQC20133.1"
                     /translation="MFYKANQNGFKEVLPGIKLKTLVYGEKSLFSEFRMTAGSILPKH
                     AHFHEQTGYLTEGKIRLTIGNETFDVEIGDSWCIPGNVEHSAEILKDAVAIEVFTPVR
                     EDYLPGNP"
     gene            complement(1193..1981)
                     /locus_tag="PLR13_12140"
     CDS             complement(1193..1981)
                     /locus_tag="PLR13_12140"
                     /inference="COORDINATES: protein motif:HMM:NF038213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQC20134.1"
                     /translation="MAAQNKGRYKSNKPLTGKTAIVTGASSGIGKATAMTLAQAGAAV
                     VIQARRKDRLVKLASDIEAKGGKALAISGDASLTSDTDRLLEKTLEWKAGGRKYDVVV
                     VNAGRGLAGGMLSSDHYQWEEVYHTNVLGAAYLMRSAAKYFVERKKGDIVAIGSVVGR
                     NISPFSAFYGSSKFAVGAIVEGLRQEVCSHGVRVSLVMPGIVKSEFQKVAGYNEDNFY
                     KGIAHMGKLLDPQAIADGIYWLLTQPRNVNISEIMIRPTGQNYP"
     gene            complement(1965..>2509)
                     /locus_tag="PLR13_12145"
     CDS             complement(1965..>2509)
                     /locus_tag="PLR13_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQC20135.1"
                     /translation="NGHKVIFHDLYKEKFDPLLYATEISEDTKLPVKIKKYCEEISAA
                     DGIIIVHPNWWGQPPAILKGWVDRIIRPGIAYEFLEGDSGEGVPNGLIKAKTTLVYNT
                     SNTKTRREKEVFGDPLETIWKNCIFGLCGITNFHRRMFNVIVTSTEKQRKIWLNHIQK
                     DIHFFFPDKRTKGGKYGGAK"
BASE COUNT          568 a          603 c          593 g          745 t
ORIGIN      
        1 tgttttttta aatacaccct gcctggtgtt ttatgcaata agaaaatccg tataggaggc
       61 gtcgggatca aaaatgccct tatttacaat cgagcatggt gccccggcag ttggcggcgc
      121 cgcagtagca ggcgtattct tttttaagcc ggggtgtttt acgggagtcg acgaccaggc
      181 tgtaatcata agagagttct tctccggctt ttatatgccg taacgctttg aggaaaacgc
      241 gattgccttt ctgttcggct tccagattag gctggcacga gtgattaatc catttcgcgg
      301 aattgccgtt atacttggcg tcgatgacgt ggtgatcgtc caggtgaaaa taaaaggtgt
      361 gatggggttg acttttgtcg tgcgggtgac gtgccagagc tttcgtccac gtgatgattt
      421 cgcctttgta ttccagaagc gtttcaccgg catcaatggg gtgaacggca aaaacccctt
      481 tgccgtggat gccggattgg cgcacctgaa tacggcgttt gccgtttgat gacggcgatt
      541 tgtcataaac tttctgtgtg tttgatctga ttatttttcc catcttcata aagcgcttgt
      601 tcatcgacaa gttttgtggg cgtgtataca aaaggcgcgt ccggtagtca agatgaaatt
      661 tacgggaccg cggattattt acgatatcag cctttgtgtc tagatatttt ttgagcggaa
      721 aaaattcttg atgccgcata ttgcagtatg gcaaggacac tgtccggaaa taccttcgca
      781 gaaacgcttc cccaacttca gtgtcacact taccgatttc gatgcccttc tgtctatgga
      841 ttgcccggaa gatagtcctc gcgaacgggc gtgaagactt caatggcaac ggcgtctttc
      901 aatatttcgg cgctgtgttc cacattgccc gggatacacc aactatcccc tatttcaacg
      961 tcaaacgttt cgttgccgat ggtgagccgg attttacctt ccgttaaata tcccgtctgc
     1021 tcatgaaaat gcgcgtgttt cggaagtatc gagcctgcgg tcattctgaa ttcagaaaag
     1081 agagactttt ctccgtaaac aagagttttg agctttatac ccggcaatac ttctttaaat
     1141 ccgttttgat tcgccttgta aaacaatcga ttctcctttt tgacgttaat cattatgggt
     1201 aattctggcc cgtgggcctg atcatgattt cgctgatatt gacgttcctc ggctgtgtga
     1261 gcagccagta aatgccgtcg gctatggctt gcggatccag taatttcccc atatgcgcga
     1321 tccctttata gaagttatct tcgttgtaac cagccacttt ttgaaattcg ctttttacga
     1381 ttcccggcat gaccagagat acgcgaacgc catgcgagca aacttcctga cgcaaacctt
     1441 ctacgattgc gccgacggca aatttgctgg agccgtaaaa cgcgctgaag ggagaaatgt
     1501 ttcttcccac aacggatcct atggcgacga tatcgccttt ctttcgttcg acgaaatatt
     1561 tggccgcgga gcgcatcaaa taagcagcgc cgagcacatt ggtatgataa acctcttccc
     1621 attgatagtg atcgctggac agcatgccgc ctgccaagcc gcgaccggca ttaactacta
     1681 cgacatcata cttacgaccg cctgctttcc attctaaagt tttctccagg agcctgtctg
     1741 tatcggacgt caggctggca tcgcccgata tggctaaggc cttgccgccc tttgcctcga
     1801 tatccgaagc cagtttgacg agcctgtctt tacgccgtgc ctgaataacg accgccgctc
     1861 ctgcctgcgc cagcgtcatc gccgttgctt tcccgatgcc ggaactggcg ccggtgacaa
     1921 ttgcggtttt tccggtcaga ggtttgttgg atttatacct gcctttattt tgcgccgcca
     1981 tatttacctc cttttgttct cttatcgggg aaaaagaaat gaatgtcctt ttggatatgg
     2041 ttcagccaaa ttttacgctg cttttccgta ctggtaacaa tgacattgaa catccggcga
     2101 tgaaaattgg tgatgccgca gagaccgaag atgcaattct tccagatggt ttccaacgga
     2161 tcgccaaaga cttctttttc tcttcgggtt ttcgtatttg atgtattgta aaccagtgta
     2221 gttttagctt tgattaaacc attgggaacg ccttcacccg aatcgccttc tagaaattcg
     2281 taggctatcc cggggcgaat gatccggtcc acccagcctt tcagaatggc aggaggctgt
     2341 ccccaccagt tgggatgaac aattatgata ccatccgcgg ctgagatttc ttcacaatac
     2401 ttttttattt tcacaggcag tttggtgtct tcagatattt ccgtggcata aagtaaagga
     2461 tcgaactttt ccttgtagag atcatgaaag attactttgt gcccgtttt
//
LOCUS       DAONQZ010000154         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_42413_length_2509_cov_13.501222, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000154 DAONQZ010000000
VERSION     DAONQZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42413_length_2509_cov_13.501222"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLR13_12150"
     CDS             complement(<1..1307)
                     /locus_tag="PLR13_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQC20136.1"
                     /translation="MKFLPAVVSSFVESKIVKANIRLLVKYLIAIIALITTYSILFHY
                     IMAMEGQEHSWITGFYWTMVVMSTLGFGDITFTSDIGRLFSIIVLLSGVILLLILLPF
                     VFIKFFFAPWMEAQTRNRAPRELPVDTRNHVIITNFDPVTEALIEKLKSYNKNYVVIV
                     EDLAKALELYDRDIRVALGNIDDPETYNKMRVQNAELIVASNSDQMNTNITLTLREDL
                     EHIPIITTAESPHSVDILQLAGSSRVLQLYDIMGESLASWTVAGECKANIIGRHKELI
                     IAQTPVIGTPLVGQTLAESKLRETFGLTVVGIWERGKFIIPKSDYKINRTSVLVLAGL
                     ESSLVQYDDVYSFYHICKIAGDPVIIVGGGRVGRAVANHFQERDIPYLIIEKSRRKNE
                     DDSHFVYGDAADIHTLEKAWFEKAPAALITSHDDATNIYLTKYL"
     gene            complement(1451..>2509)
                     /locus_tag="PLR13_12155"
     CDS             complement(1451..>2509)
                     /locus_tag="PLR13_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20137.1"
                     /translation="LLGFLPLVEIFNEAIVGMVALGLIFTTLMSKMQLPGRMPGVLAA
                     VILGTGIHFFLGFSGYLPEFAAPTLDMHFALPVPSVEFLKTLPQSVHYLTIAIPFGIL
                     TIIGGINNTESARLAGDDYRTRDILLTEALSSLIASFFGGVAQTTPYIGHPAYKKMGA
                     TWWYTLLTGILIGVCSIIGLLSVFVSLIPRAVIAPIFIFIGFEIMRQAYKDSPAAHSP
                     AVSLSILPVIASMVLIVLGQFTGASGIILEKLPGRLLIMHQTLTMLSNGFIITGLLWG
                     SMLAFLIDHQAKRAALCAAVSAILTLFGIIHSVLPTGELYLPWHIQSQAHYMTALAYF
                     TLSAILLLFNPPKAEAQT"
BASE COUNT          628 a          634 c          678 g          569 t
ORIGIN      
        1 agatattttg tgagataaat attggtggcg tcgtcatgcg atgtgatcag ggcagcgggc
       61 gctttttcaa accaggcttt ctccagagta tgaatgtcgg cggcatcccc atatacgaaa
      121 tgcgaatcat cctcattttt ccggcggctt ttttcaatga tcagataggg aatgtcccgc
      181 tcttgaaagt gattggccac ggcgcgtcca acccttcctc cgccgacgat gatgaccgga
      241 tcgccggcaa ttttgcagat gtgataaaat gaatagacgt catcatattg gacaagcgag
      301 gattccaggc ccgccagcac cagcacgctg gttcgattga ttttataatc ggatttcgga
      361 ataatgaatt ttccgcgttc ccagattccc accaccgtta agccgaaggt ttcgcgaagt
      421 ttactttcgg caagcgtctg accgaccaga ggggtgccga tgaccggtgt ctgggcgatg
      481 atcaattcct tgtgccggcc gatgatattg gctttgcatt ctccggcgac agtccaactg
      541 gccagagatt ctcccatgat atcgtaaagc tgcaagaccc gtgagcttcc cgccagttgc
      601 agaatgtcca cggaatgagg ggactccgcc gttgtgataa tcggaatatg ttccagatcc
      661 tcccggaggg ttagggtgat attcgtgttc atctggtcgc tgtttgatgc cacaatcaac
      721 tcggcattct gtacgcgcat tttgttgtat gtttcgggat catcgatatt gccgagcgca
      781 acgcgaatat cccggtcgta caattccaga gccttggcca gatcctccac gatcaccaca
      841 tagtttttat tgtagctttt cagtttttca atcagagcct ccgttaccgg atcgaaattg
      901 gtgataatga catgatttct cgtgtccacg ggaagttccc gaggcgcgcg gtttcgggtt
      961 tgcgcttcca tccagggcgc aaaaaagaat ttgatgaaga cgaaaggcag aagaatcagc
     1021 agcagaataa caccggaaag cagaacgatg atggaaaaaa gtcttccgat gtcactggtg
     1081 aacgtaatgt ccccgaaccc cagtgtggac atgaccacca tcgtccagta aaatccggtg
     1141 atccacgagt gttcctgtcc ttccatggcc atgatgtaat ggaacaggat gctgtaggtg
     1201 gtaatcagcg caataatcgc aatcagatat ttcaccagaa gacgaatgtt ggctttgaca
     1261 attttactct cgacaaaaga actgacgact gcgggtaaaa atttcatacg gttggcatcc
     1321 ttgagacgga actgattttg agcgacgtga tttgtctgcg tattgcaaag tatagaaaga
     1381 accgttttgc ctgtcaattg aaatcatcac gggcaaaacc gcaccggacc ctcgccttcg
     1441 ccaatcgaca ttatgtctgt gcctcagcct ttggaggatt gaatagaagc aggatcgcgg
     1501 acagggtgaa atatgccagg gccgtcatat aatgcgcctg agactgaatg tgccacggca
     1561 gatacagttc gcccgtcggc aaaaccgagt gaatgatgcc gaagagggtc aggatggcgc
     1621 tgaccgcggc gcataaagcc gcacgtttcg cctgatggtc gatcaagaag gccagcatgc
     1681 ttccccagag aagccccgtg atgatgaatc cgttgctgag catcgtcagc gtctgatgca
     1741 tgatcagcaa tcgtccgggg agtttttcca ggatgatgcc cgacgcgccc gtgaactgtc
     1801 ccagaacaat cagcaccatg cttgcgataa ccggcagaat gcttaagctg accgccgggg
     1861 agtgggcggc cggcgaatcc ttatacgcct gccgcatgat ctcgaaaccg ataaagatga
     1921 aaatcggcgc aatcacggcc cgcggaatca ggctgacaaa aaccgaaagc agcccgatga
     1981 tggagcagac gccgatcagg atgcctgtca gcagcgtata ccaccaggtg gcccccattt
     2041 ttttgtaagc cggatgaccg atgtagggtg tggtctgcgc cacaccgccg aagaaggacg
     2101 cgatcagcga ggacagcgcc tccgtcagca gaatatcgcg ggtgcggtaa tcgtctccgg
     2161 ccagacgcgc gctttcggtg ttgttgatac cgccgatgat ggtcagaatc ccgaagggaa
     2221 tggcgatggt cagataatga acgctctgcg gcagggtttt cagaaattcg acggacggca
     2281 ccggcagagc aaagtgcatg tccagagtcg gcgcggcaaa ctccggcagg tatccgctga
     2341 atcccaggaa aaaatgaatt ccggtgccta aaattaccgc cgccagcacg ccgggcatcc
     2401 gtccgggcag ttgcattttg ctcatgagag ttgtgaagat cagccccagc gccaccatgc
     2461 cgacgattgc ttcattgaaa atttcaacaa gcggcaagaa gccgaggag
//
LOCUS       DAONQZ010000155         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_44434_length_2426_cov_12.176297, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000155 DAONQZ010000000
VERSION     DAONQZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44434_length_2426_cov_12.176297"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLR13_12160"
     CDS             complement(<1..406)
                     /locus_tag="PLR13_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQC20138.1"
                     /translation="MSNKGLKPREIVEKLNQHIIGQMDAKRAVAIALRNRWRRQNVLP
                     ELAEEILPKNIIMIGPTGVGKTEIARRLAKLDNSPFLKIEASKFTEVGYVGRDVESMV
                     RDLVELSVNMVKAEEQESVQAKAAEIAEERVLD"
     gene            complement(410..991)
                     /gene="hslV"
                     /locus_tag="PLR13_12165"
     CDS             complement(410..991)
                     /gene="hslV"
                     /locus_tag="PLR13_12165"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQC20139.1"
                     /translation="MNIRGTTILAVKRDGKITVAGDGQVTLDTTILKHGARKVRRLYN
                     NEVIVGFAGATADAFTLFDRFDQKLEQYKGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VNRDNSLIISGNGDVIEADDDVMAIGSGGPFALTAARALLRHSSLNSTEIAREAMKLA
                     SEICIYTNEHITLEEIDIPSEDRERKGKDKGKK"
     gene            complement(1137..2054)
                     /gene="xerC"
                     /locus_tag="PLR13_12170"
     CDS             complement(1137..2054)
                     /gene="xerC"
                     /locus_tag="PLR13_12170"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQC20140.1"
                     /translation="MGNMIRDFQTYLDVEKNVSEHTKTAYIADIQEFARFLVEQQFVR
                     QPDEIVSTQPETLRHYLAVLYKKKMKKVTVNRKIASLRAFFKYLMRTGKIDVNPAEMV
                     QSLKTEKYIPTFLSVDEMLEVLNAQRDNSFSGLRDRAMLEVFYSCGLRLSELAGLDVA
                     DIDFNQKLVKVRGKGRKERIVPVGDPALKALTDYLEKARLIRTKSEPGVFKMPVFLNQ
                     RGERITTRSIARIVNASTVKSGIGRKISPHALRHSFATHLLNAGADLRSIQELLGHES
                     LSTTQKYTAVDINRLMEIYDKAHPRTKKP"
BASE COUNT          515 a          720 c          513 g          678 t
ORIGIN      
        1 aatccagaac cctttcctcg gcgatctccg ccgctttcgc ctgaacgctt tcctgttcct
       61 ccgccttgac catgttcacg gacagttcca ccagatcccg caccatggat tccacatccc
      121 ggccgacata ccccacttcc gtaaatttgg acgcctcgat ctttaaaaac ggggaattat
      181 ccagcttggc cagccggcgg gcgatttccg tttttcccac gccggtgggg ccaatcatga
      241 taatgttttt cggtaaaatt tcctccgcca gttccggcaa tacattctgc ctgcgccagc
      301 ggttgcgcaa agcgatggcc actgcccttt tggcgtccat ctgaccgata atatgctgat
      361 tcaacttctc cacgatttcc cgtggtttta atcctttatt agacatgttt tacttctttc
      421 ctttatcctt cccttttctt tccctgtcct cggaagggat atcgatttct tccagggtaa
      481 tgtgctcatt ggtgtaaata cagatttccg aagccagctt catcgcctct cgggcgattt
      541 ccgtcgaatt caaactggaa tggcgcaaca gcgcccgtgc cgccgtcagc gcaaacgggc
      601 cgcccgaacc gatcgccatg acatcgtcat cggcttcaat gacgtcgccg ttgccggaaa
      661 taatcagaga attgtccctg ttgacggcga tcatcagggc ttccagatgt cttaaaaccc
      721 ggtcggtgcg ccagtccttg gtcagctcca cggccgctct gagcaaattg cctttgtact
      781 gctccagttt ttgatcgaaa cggtcgaaca gcgtaaacgc atcggcggtt gctccggcaa
      841 agccgacgat cacttcatta ttatacaggc ggcggacctt gcgcgcgccg tgcttcagga
      901 tcgtcgtgtc cagcgtcacc tgtccgtctc cggcaaccgt tattttaccg tcccgtttca
      961 ccgccagaat ggttgtgccg cgtatattca tttcttctcc ttaaataaaa attattccgg
     1021 aaacaacaac aaaaatcgga cgaaggcttt tctctgcggc ttcgccttga aaaaagccaa
     1081 gtgcccttgt gttttcagat cccgattccc gcttcgcgcg aacgggataa attcgactaa
     1141 ggctttttcg tccggggatg cgccttatcg taaatctcca tcaaccggtt gatatccacc
     1201 gccgtatatt tctgtgttgt cgaaaggctc tcatgcccga gcaattcctg aatcgaccgt
     1261 aaatcggcgc cggcattcaa cagatgggtc gcaaaactgt gccgcagcgc atgcggactg
     1321 attttccgcc cgatcccgct ttttaccgtc gacgcattga cgattctcgc gatacttctt
     1381 gttgtgatcc tttcacctct ttgattcaga aacacgggca ttttgaacac acccggttcg
     1441 ctttttgttc tgatcagacg tgccttctcc agataatctg tcagggcctt cagagccggg
     1501 tctcccaccg gaacaattct ttcttttctg cccttgcccc tgaccttgac cagcttctga
     1561 ttgaaatcga tgtccgccac atcgagcccg gccagttcac tcaggcgcag tccgcaggaa
     1621 taaaagacct ccagcatggc gcggtcgcgc agtccgctga acgaattatc acgctgcgcg
     1681 ttcaaaacct ccagcatttc atctacggac aaaaacgttg gtatatactt ttcggttttc
     1741 agcgactgca ccatttcagc gggattcaca tcgatctttc ccgtgcgcat cagatatttg
     1801 aagaaagccc gcagcgacgc tattttccgg ttcaccgtga ccttcttcat ctttttttta
     1861 tacaacaccg ccaggtaatg cctgagcgtt tccggttgcg tcgagacgat ttcatccggt
     1921 tgtctcacaa actgttgctc gacgagaaac cgcgcgaatt cctgaatgtc cgcgatataa
     1981 gccgttttgg tatgttcgga aacatttttt tccacgtcca gatacgtttg aaaatcccga
     2041 atcatgtttc ccataacttt tattccggct agccgtcaga cacccatcct gataacccgg
     2101 cagaacacct tcaatcccca ttcgcttcgc tccggggata attcccaatc ccgacaaatc
     2161 ggaattggat agttcgattt acgctttgaa tttcgtttca ctcattctca gcgaatctca
     2221 ctacggcttg cgcttcagac ttcgctgcgc tcgtctttag cgagcctcac ccacattcaa
     2281 tacgtttcaa gtcttaaatc cccattcgct tcgctccggg gataattccc aatcccgaca
     2341 aatcggaatt ggatagttcg atttacgctt tgaatttcgt ttcactcatt ctcagcgaat
     2401 ctcactacgg cttgcgcttc agactt
//
LOCUS       DAONQZ010000156         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_44921_length_2407_cov_20.184524, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000156 DAONQZ010000000
VERSION     DAONQZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44921_length_2407_cov_20.184524"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..694)
                     /locus_tag="PLR13_12175"
     CDS             complement(137..694)
                     /locus_tag="PLR13_12175"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQC20141.1"
                     /translation="MAFHPYAGFWRRLVAFTIDGTIICFIFIFLFFIASMAFFSGAVS
                     ANGEKFLASLGNAESLSPAMISLWLFYILLNIGYFTYFHGATGRTPGKSLLGMQVVST
                     EGTPISFGTAFLRSVGYLVSNILYLGFIWVAFDRRKQGWHDKIAGTVVIRRESYEQSA
                     GVSIAEPTWSPPKAQQTKPPETNEN"
     gene            complement(696..1463)
                     /locus_tag="PLR13_12180"
     CDS             complement(696..1463)
                     /locus_tag="PLR13_12180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQC20142.1"
                     /translation="MTVPRIYSPESLENKNSCALGEDNVKYLKKVMRLKEGDRIHIFD
                     GFGREYEATIKNFSAKSVLVDLGKAIPSVNRDISITLAQAITKGKKMDTIVKSAAELG
                     ADMIIPFHAVRSVSHIADEKNPRKQTRWQKIAHEAARASHSAYITEVTKILSFPEMLS
                     RCSPKALKLIFWEEESRKTIKDVLTDDALEACRDFFIVVGPEGGFSKDEIEKTKEDGF
                     FSVSLGRQILKVETAATAIISIIQYEKGIFSSTSQEA"
     gene            complement(1519..>2407)
                     /gene="prmA"
                     /locus_tag="PLR13_12185"
     CDS             complement(1519..>2407)
                     /gene="prmA"
                     /locus_tag="PLR13_12185"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQC20143.1"
                     /translation="ELMEALGNFLTESGVQGVFQESQSSQAVGSDFPEPVAEDILKAY
                     FPLDSRSDKKLAALQKYIDSLAEIFPDLGKPSFTTETICDPDWGEQWKKYFKPIRVSN
                     NIVVKPTWERYTPSSRDIVIEIDPGMAFGTGQHASTRMCIEAVEDIIMNDRSIKEWKV
                     LDVGCGTGILGITAAKMGAQDVLCADIDRKAVEIAGENAAINDVRHAVRTINKNLAAI
                     DKPRNLIIANLTSNLLLTHRQHLTQLLMPDGYMILSGIIEQDAGKVAQEYAATPLELQ
                     RTITEKEWVCYVLKKTSAC"
BASE COUNT          539 a          585 c          607 g          676 t
ORIGIN      
        1 tgccgacact cggattttca gtccgatgct ctaccgactg agctaccgac ccgattggtt
       61 aaaaatactg actccgtcta tagaataagc gtccttttgt caaggccttt ttgtgaaatt
      121 cccggctgtt tgccgtctaa ttttcattag tttcaggcgg ttttgtctgt tgtgccttcg
      181 gtggcgacca tgtcggctcg gcgatggata cgccagcgct ttgttcgtaa ctttctctcc
      241 tgatgacaac cgttccggca attttgtcat gccatccctg ttttcgtcga tcaaatgcaa
      301 cccagataaa accgagatat aagatatttg ataccagata gcccaccgat cttagaaacg
      361 ctgttccgaa agaaatgggt gttccttccg tggaaacaac ctgcatgccc agaaggcttt
      421 tccccggtgt gcggcccgtc gcgccatgaa aatatgtaaa ataaccgata ttgagcagaa
      481 tataaaataa ccacagagaa atcatcgcgg gagaaagaga ttcggcattg ccgaggctgg
      541 ctagaaattt ttcaccgttg gctgatacgg cgccgctgaa gaatgccatt gaggcaatga
      601 agaaaagaaa gataaagatg aaacagataa tggtaccgtc gatggtaaag gcgaccagac
      661 gtcgccagaa acctgcgtat ggatgaaaag ccatgtcatg cctcctgtga tgttgaactg
      721 aagattcctt tttcgtattg aatgatggat atgattgccg ttgccgctgt ttctactttt
      781 aagatttgtc tgcccaggct gacggaaaag aacccgtcct cttttgtctt ttcaatttcg
      841 tcttttgaaa aaccgccttc cgggccgacg acaataaaaa aatccctgca ggcctccaga
      901 gcgtcatccg tcaggacatc cttgatggtt ttccgggatt cttcttccca gaaaatcagt
      961 ttcaaagcct tgggagaaca ccgggaaagc atttccggaa aagataaaat ttttgtaacc
     1021 tccgtgatgt aagcgctgtg gctcgcccgg gcggcctcgt gcgctatttt ctgccagcgg
     1081 gtctgtttcc ggggattttt ttcatcggca atatggctga ccgaccgcac ggcatgaaaa
     1141 ggaataatca tgtccgctcc cagttcggcg gcgcttttaa cgatcgtgtc catttttttc
     1201 cctttggtga tggcctgcgc cagggtgatg ctgatgtccc tgttaacgga gggaatcgct
     1261 ttccccaagt cgacgagcac cgattttgcc gaaaaatttt tgatggtcgc ttcgtattcc
     1321 cgcccgaagc cgtcaaaaat atgtatccgg tcgccctcct tgaggcgcat gacctttttc
     1381 aggtatttta cattgtcttc tcccagcgcg cagctgtttt tattttccag agattccgga
     1441 ctgtatatac gggggacggt caaggcctgt tttcctttat agatggttta tctgaagatt
     1501 gccgtttgca gggtgctgtc agcaggcgga tgtctttttg agcacgtaac agacccattc
     1561 cttttccgtg attgtccgtt gcagttcaag aggtgttgcg gcatattcct gcgcgacttt
     1621 tccggcatct tgttcaatga tgccggaaag gatcatgtag ccgtccggca tcagtaattg
     1681 cgtcagatgc tgccggtggg tcagcagtag attcgacgtg aggttggcga taatcagatt
     1741 acggggtttg tcaatggcag ccagattttt attgatggtg cggacggcgt gccgcacatc
     1801 attaatggcc gcgttttcac cagcaatttc gacggctttc cggtcgatat ccgcacagag
     1861 aacatcctgt gctcccattt tggcggcggt aatgccgagg atgccggtgc cgcaaccgac
     1921 atccagcact ttccattctt taatggaacg gtcgttcatg atgatatctt caaccgcttc
     1981 aatgcacatt ctggtggacg catgctggcc ggtgccgaat gccatgccgg gatcgatttc
     2041 aataacgatg tctcgactgg aaggcgtgta tcgttcccat gtcggtttga caacgatgtt
     2101 gttgctgacc cggatgggtt taaaatattt tttccactgt tctccccagt cgggatcgca
     2161 gatggtttcg gttgtaaatg acggttttcc cagatcgggg aatatttcgg ccaggctgtc
     2221 gatgtatttt tgcagtgccg ccagtttttt atcgctgcgg ctgtcgagcg ggaaataggc
     2281 tttcaggatg tcttccgcaa ccggttcggg aaagtctgaa ccgacggcct gagaggactg
     2341 cgattcctga aacacgccct gaacaccgga ttccgtgaga aaatttccca gcgcctccat
     2401 cagttcc
//
LOCUS       DAONQZ010000157         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_45075_length_2401_cov_14.296249, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000157 DAONQZ010000000
VERSION     DAONQZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45075_length_2401_cov_14.296249"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..882)
                     /locus_tag="PLR13_12190"
     CDS             complement(184..882)
                     /locus_tag="PLR13_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20144.1"
                     /translation="MHCCRLSRIGIPTEFWVAKKEAFPEGAEPTLLYKDGGKLMEITI
                     SDNPGLKKGSAYDENSMLFKLLRRTVIRLKDEFLQVWNGADAEGVLFNLNKFPCYWEM
                     TDEQQEKLHALNKRLMDVEKSIHERYGQIHKDHPEGELSCEIDFLLKEDDPEWSDEDD
                     NIMASMDHSFDEYDPNASWNDASHLIRDKDGKPLHLCWLFHELYDHLGLVWEDLLRVG
                     QIRVTVQLNYYYHY"
     gene            complement(1102..1317)
                     /locus_tag="PLR13_12195"
     CDS             complement(1102..1317)
                     /locus_tag="PLR13_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20145.1"
                     /translation="MDEFAGTEKYGFRHRKIRHHEAGIAEVCRIFGQEAGAAARQHII
                     SDLKADGWSDGDPFPKDENDYVKIGFF"
     gene            complement(1397..>2401)
                     /locus_tag="PLR13_12200"
     CDS             complement(1397..>2401)
                     /locus_tag="PLR13_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HQC20146.1"
                     /translation="YIRKGTLDVQTLFTSEDITKQVKAKQPFVVKQLRKQRRMLNGEL
                     PKIDIGPHCSDPYACDFQEYCWRHIPDNSVFDLRRKGIDPYDLYAQGIIKQKDIPLDL
                     LNEKQRQQVVATINKENTIDKKKIRAFFKTLSYPLYFLDFETFMSVIPPYDGLKPYQN
                     VPFQYSLHFQKKKGGKLFHTEFLAQPGIDPRMPLLKKLLADIPEDVCILTYNMAFEKK
                     VLTELAAQFPVHKKTIGKWINNVRDLMIPFRQRDVYFWKFKGSFSIKKVLPALVPELS
                     YEGLAIADGGAAMDAYQKMLAAMDDQKKLSEIRKNLLAYCQLDTLAMVRILEALEKLE
                     "
BASE COUNT          608 a          578 c          492 g          723 t
ORIGIN      
        1 gcaaaagtat tcttagcaaa tttgaattgt gctggagggc gtatagcacc tacaagtcag
       61 tacatttgac gctctggtta aatcaattat tacgggaaga aagtgtgcac attccggtag
      121 gctatttcac agatgctgaa atccggattt ctttgcttag tcatgcaaca cctcaccttg
      181 gcttcaataa tgataataat agtttaactg aacagttacc cgtatctgtc cgacacggag
      241 caagtcttcc cagacaagtc ccaaatggtc gtaaagctca tgaaacaacc agcacagatg
      301 caagggtttt ccatctttat ccctgatcag atgagaagcg tcattccagg aggcgttggg
      361 gtcgtattca tcgaatgaat gatccatact cgccataatg ttgtcgtcct catcgctcca
      421 ttccgggtcg tcctccttca gcaagaaatc tatctcgcag gataactctc cttccgggtg
      481 atctttatgt atttgcccgt atctttcatg aatggacttc tccacgtcca tgagtcgttt
      541 attcagggca tgaagttttt cctgctgctc atcggtcatt tcccaatagc atggaaactt
      601 gttcaaatta aacaagacac cctcggcgtc agcgccattc catacctgca gaaattcgtc
      661 tttcaagcgg atgacggttc tcctgagcag cttgaagagc atggagtttt catcgtatgc
      721 gcttcccttt tttaacccgg gattatccga gatcgttatt tccatcagct taccgccatc
      781 tttgtaaagc agtgtcggtt ccgcaccttc gggaaaagct tcctttttcg ccacccagaa
      841 ttccgtcggg atgccgatgc gggacaagcg acagcaatgc ataagatact tagcgttctt
      901 tacagcgata atgttgttaa tgacacatcc ctcctttctc gattaatttg cctgaaactc
      961 cgacatgcgc agagctatct cttggcgcag agcttctatt tgaactcttc attttcaagc
     1021 tgtcttggct gatgaatgat tcgggattaa ttcccaaact tttagaggcc agcatattaa
     1081 aatctgattt cgttcatcgg actaaaagaa accaattttc acataatcat tttcgtcttt
     1141 cgggaacgga tctccatcgc tccatccatc tgcttttaaa tcggaaatga tatgctgtct
     1201 tgccgccgcc cctgcctctt ggccgaagat tcggcaaact tcagcgattc cggcttcatg
     1261 atggcggatt tttctgtgtc tgaatccgta tttctcagtc ccggcaaatt catccaacca
     1321 cagatggacc ttcatataag attttccgaa gaggttcact gattcccgac aatgatcttc
     1381 aagacacgac ataacgctac tccaatttct caagcgcttc taaaatgcgc accatcgcca
     1441 gcgtatccaa ttgacagtaa gcaagtagat tcttccttat ttctgacagc tttttctgat
     1501 cgtccatcgc cgccaacatt ttctgatagg catccatagc cgcgccgccg tcagcaattg
     1561 ccaatccttc gtaggaaagt tccggcacca gggccggcaa cacttttttg atggaaaatg
     1621 atcctttgaa tttccagaag taaacatccc tctgtcggaa agggatcatc agatctcgaa
     1681 cattatttat ccatttccca attgtcttct tgtgcacagg aaactgggca gccaactccg
     1741 tgagcacctt cttttcaaag gccatattgt aggtcaggat gcagacatct tccggtatat
     1801 cggccagcag tttctttaaa agcggcatac gcgggtcaat tccgggttgc gccagaaatt
     1861 cagtatgaaa aagtttgccc cctttctttt tctggaaatg cagagaatac tggaaaggaa
     1921 cgttttgata gggtttaagg ccatcgtaag gcgggatgac gctcatgaat gtttcaaaat
     1981 caagaaagta gagaggatag gaaagtgttt tgaaaaatgc cctgatcttt ttcttatcaa
     2041 tcgtgttttc tttattgatc gttgccacaa cctgttggcg ctgtttttca ttcagcaaat
     2101 caagcgggat gtctttttgc ttgattattc cctgtgcata taaatcataa ggatctattc
     2161 ctttgcggcg cagatcaaat acagaattat ccggaatgtg tcgccagcaa tattcctgaa
     2221 aatcacatgc atacgggtca gaacaatgcg gtccgatatc gattttgggt agctcgccat
     2281 tgagcattcg cctctgcttc ctcaattgtt tgacgacaaa cggctgtttt gcttttacct
     2341 gtttggttat gtcctcgctg gtgaagagcg tttgaacatc caacgttcct tttctgatat
     2401 a
//
LOCUS       DAONQZ010000158         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_45543_length_2383_cov_15.620275, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000158 DAONQZ010000000
VERSION     DAONQZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45543_length_2383_cov_15.620275"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1092)
                     /locus_tag="PLR13_12205"
     CDS             complement(265..1092)
                     /locus_tag="PLR13_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HQC20147.1"
                     /translation="MNDLQKMEHNQIVVFGSKKIRRIWHDEEWYFSVIDICGALTNSP
                     DAGAYWRKLKQRLNVEGSEVVTFCHGLKLEAADGKKYATDCANTESMFRIIQSIPSPK
                     AEPFKRWLARVGYERVQEIENPELAQERMKLLYEQKGYPRDWIDKRLRGIAIRQNLTD
                     EWRERGIISERDYAILTAEISKATFGITPADYKKLKGLFKKNQNLRDYMTDLELIFTM
                     LGEKVTTEISQKEKPDTFEKNRKVAKRGGGVAGTARKETEKELGRSIITNQNYLKEK"
     gene            complement(1251..1838)
                     /gene="thpR"
                     /locus_tag="PLR13_12210"
     CDS             complement(1251..1838)
                     /gene="thpR"
                     /locus_tag="PLR13_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQC20148.1"
                     /translation="MTADNEKNIRAFLAIEPPENILNEISRLQEKLKREINGRLSWTR
                     PQGQHLTLKFFGDISKEDINSICATVQKRVVAEQKLNLKVEKMGVFPDARRPRVLWCG
                     VTGDVEKLITLQKKLDADFATLGFLEEDRSFKAHLTLARIKDPRDITGMSEALKKYDS
                     FKAGEFVADKLFLFQSKLSPQSAVYTKLAEFALGG"
     gene            complement(2071..>2383)
                     /locus_tag="PLR13_12215"
     CDS             complement(2071..>2383)
                     /locus_tag="PLR13_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQC20149.1"
                     /translation="GVPAQIIEKHGAVSEETARLMAEGVRKLAGTTLGLSSTGIAGPT
                     GGTKEKPVGTVYLAVADAQKTICRHYTFRWDRKRNKEVFSEAALFLLKNFCETQIAKT
                     K"
BASE COUNT          564 a          595 c          467 g          757 t
ORIGIN      
        1 actgtttttc tatctgagta atcgccaaat caacggcctt tgatctatcc atatccgcca
       61 taatacaatg ccctccttat tttcttttta tattaataaa tgcttaaaaa ttacaactct
      121 aatttatgaa aacaacttat gctcattata tcctcaaagc aaactccgcc agttttgtgt
      181 aaacagcgcc ccgcggcgca agtgcttatg tttggaccaa cttatttctg caaccagtgg
      241 ttgcagtttt tcatacgctt taatctattt ctccttcaaa taattctgat tagttatgat
      301 actgcggcct aattcttttt ccgtttcttt gcgcgccgta cctgccacac cgcctccgcg
      361 tttggccacc tttctatttt tttcgaaagt atccggtttt tctttttgcg atatttccgt
      421 ggtaactttt tcgccaagca ttgtaaaaat caactccaga tcagtcatgt agtcccgcag
      481 attctgattc tttttgaaca atccttttaa cttcttatag tccgctggcg ttatcccaaa
      541 agttgctttt gatatttcag ccgtaagaat ggcataatcc cgttcgctta taatgcctct
      601 ttctctccac tcatcggtca ggttttggcg aatggctatt ccgcgcaacc gtttatcgat
      661 ccaatccctg ggataacctt tttgttcata caataatttc atgcgttctt gcgccagttc
      721 aggattttca atttcctgta cccgctcata cccgactctt gccagccaac gcttgaacgg
      781 ctcagctttc ggtgaaggaa tggattggat gatacggaac atactttctg tgttggcgca
      841 gtcagtagca tattttttgc cgtcggcagc ttctaatttc agaccgtgac aaaatgtcac
      901 gacctcactg ccttctacgt tcaaacgttg tttcaatttt ctccaataag cgcctgcatc
      961 agggctgttc gtcaatgcgc cgcaaatatc tataacagag aaataccatt cctcatcatg
     1021 ccagattcgc ctgatctttt tacttccaaa aacaactatt tgattgtgtt ccatcttctg
     1081 taaatcattc actgtatttt tcttttaacc tcctgcttaa ttcggagatg ctatacttat
     1141 tttctggatt cccactcgga ggagggaatg acagccgggg agtatgttcc caaatggaac
     1201 atactgaact ttcatttcga actgtttcaa aaaatgcaac agttcgaatg ttatccaccc
     1261 aaagcaaact ctgccagttt ggtgtaaaca gcgctctgcg gagaaagctt gctttgaaat
     1321 aagaacaatt tatcggcgac aaattctccg gctttaaacg aatcatattt tttcagcgct
     1381 tcgctcatgc ctgtaatgtc acgcgggtct ttgattcgcg ctaacgtcag atgcgccttg
     1441 aaagaccggt cttcctcaag aaagcctaaa gtcgcgaaat cggcatcgag ttttttctga
     1501 agagtaataa gtttttccac atcgcccgtt acaccgcacc acaaaacacg cggtctgcgg
     1561 gcatccggga aaacacccat cttttcaact ttcaaattca gtttctgttc ggcaacaact
     1621 cgcttctgca cggtggcgca aatactgttt atatcttcct tcgaaatatc gccgaaaaat
     1681 tttaaggtca gatgctgacc ctgcggcctt gtccagctga gccggccgtt gatttcccgt
     1741 tttagttttt cctgaaggcg ggagatttca ttaagaatat tttccggcgg ttcaatcgcc
     1801 agaaaggcgc ggatattttt ttcgttatcg gctgtcatac tctaaacttt ccttatgttc
     1861 tgtcgtcttc ccggtcgtag atgaccttta ggttacgaag gccgggaacc agttcataat
     1921 tcctggatgc cggatcaagt ccggcatgat aaagggataa ttttcgtgac gcttcctata
     1981 gtcagcgagt gtcattaagc tttctcctgc aaatcacggg gactatagta ccctccgctg
     2041 tgcgggattg ttcgacttac aaatttcaat tcacttcgtt tttgcaattt gagtctcaca
     2101 aaagtttttc agcaaaaaaa gcgccgcttc ggagaaaacc tccttgttcc ttttgcggtc
     2161 ccagcgaaag gtgtaatggc ggcaaatcgt cttttgtgca tccgcgactg ccagataaac
     2221 tgtgcccacg ggtttttcct tagtgccgcc ggtcggtccg gcaatgccgg tagaggaaag
     2281 tcccagagtg gttccggcca gtttgcgtac gccttcggcc atcagtcgtg ccgtctcttc
     2341 gctgaccgcg ccatgctttt cgatgatttg agcgggaaca ccc
//
LOCUS       DAONQZ010000159         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_45617_length_2380_cov_14.983226, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000159 DAONQZ010000000
VERSION     DAONQZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45617_length_2380_cov_14.983226"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1241
                     /locus_tag="PLR13_12220"
     CDS             108..1241
                     /locus_tag="PLR13_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC20150.1"
                     /translation="MKFIRLKAIVRKEFYHLIRDYRSLYLAFAIPLLLIILFGYALSL
                     DVDNVETAIVDYDKTELSRDFIRKLDASAYFHVSGILAGTKEATRSLDHDRVKIAIII
                     PPDFKKNIGADRETPLQIIIDGSDPNFANIIRGYITSFVSHYNQKLLMNFLNRQGMQE
                     IKPPVDGRIRIWFNEDLESRNFIIPGIIAVIIMIVGALLTSLVIAREYENGTMETLKS
                     LPIEAGELLLGKAFPYFCIGLTDVLVSILLGQLLFGIVMKGNFWLMILSSSLYLSVAL
                     SLGLLISNITKSQLMANQIAILTTYLPSFLLSDFVFPIINMPKIIQAITLVIPARYFI
                     NILSGIYLRDLGLMYLWPSLLVLLVMFSVLTFFNYKMLKKEGL"
     gene            1238..2359
                     /locus_tag="PLR13_12225"
     CDS             1238..2359
                     /locus_tag="PLR13_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQC20151.1"
                     /translation="MSWLRIRELVRKEFIQLFSDKRNRAVMFVMPFIMMLVFGYVVNY
                     DIRNVRIAVLDYSKTHESRSLIDCFTGSKIFHVTHHVTRENQMSELLLRKKIDVGIKI
                     SQDFAPVVRAGQTAPVQFLVDGSMSNMTAVRVAYIASVLDKFNREELRRLYPMHMSYG
                     RVDARIRTWYNPNLDSQYFFVPGIVAFLIMLTSFIFTSIAIIREKEDGTMEQLIVTPI
                     KSHEFIIGKTIPYTIIALFQLFAVTGVAVYWFEIPFKGSFLLLLLAACLFLLSTLGIG
                     LFISTISSTKQQAMMTAFFFVLPFFMLSGFVFPISNMPDVVQWLTYLNPLRFFLVIIR
                     DIFLKGVGFDVLWPQYLALTILGAVVFTGAIARSKKRLD"
BASE COUNT          549 a          593 c          549 g          689 t
ORIGIN      
        1 attgtggcat cgggaacgcc cgcggaaatt atttccggcg tctgcccgga tatgcccgat
       61 gccgatttaa cggatgcgtt tatcaagttg atgaagaggg aagaaatttg aaattcatca
      121 gactcaaagc catcgtccgc aaagagtttt accatttgat ccgcgactat cgcagccttt
      181 atttggcgtt tgccatcccc ttgcttttga ttatcctttt cggctacgcg ctgagcctgg
      241 atgtcgacaa cgtggaaacc gccattgtcg attacgacaa aacagaactc agccgcgatt
      301 ttatccgcaa actcgacgca tccgcctatt ttcatgtgtc gggcatcctc gccggcacga
      361 aggaggccac cagatctctg gatcacgatc gggtaaagat agcgattatc atcccgccgg
      421 acttcaaaaa aaacattggc gccgaccggg aaactccgct tcagattatt atcgacggca
      481 gcgaccccaa tttcgccaat atcatccgcg gatatattac ctccttcgtt tcgcactata
      541 atcaaaaact tctgatgaat tttctgaacc gtcaggggat gcaggaaata aaacctcccg
      601 tggacggacg cattcgcatc tggttcaatg aggatttgga aagccggaat ttcattattc
      661 cgggcatcat tgccgtcatc attatgatcg tcggcgcatt gctcacctct ctggtgatcg
      721 cccgggaata cgagaacggc acgatggaaa ccctcaaatc cctgccgata gaagcggggg
      781 aactgctttt agggaaggcc tttccttatt tttgcattgg attaacggac gtgctggtat
      841 ccattttact gggacaatta ctgttcggca tcgtgatgaa aggaaatttc tggctgatga
      901 tcctctcctc ctctctttat ttaagcgtcg ccctatccct cgggcttttg atttccaata
      961 tcacgaaatc gcaattgatg gccaaccaga tcgcaatcct gaccacctat ttgccttcat
     1021 ttcttctgtc cgattttgtt tttccgatta tcaatatgcc gaaaatcatt caggccatca
     1081 ccctggtcat cccggcgcgg tatttcatta atattttatc gggaatctat ctgcgcgatc
     1141 ttggattgat gtatttgtgg cccagcctgc tggttttact ggtgatgttt tccgtactga
     1201 ctttttttaa ttacaaaatg ctgaagaagg aaggtctatg agctggctga ggatcagaga
     1261 actcgtgcgc aaggaattta ttcagctttt cagtgataag cgcaatcggg cggtgatgtt
     1321 tgtcatgccc tttattatga tgctggtttt cggttacgtg gtgaattacg atatccggaa
     1381 cgtccggata gcggtcctgg attattccaa aacccatgag agccgatcgc tcatcgactg
     1441 tttcacaggc agtaaaatct tccacgtaac gcatcatgtg acccgtgaaa accagatgtc
     1501 ggaactgttg ctcagaaaaa aaatcgacgt aggaatcaag atcagccagg atttcgcgcc
     1561 ggtggtcaga gcgggacaaa cagcgccggt ccagtttctt gttgacggtt ccatgagcaa
     1621 catgacggct gtccgggtgg cttatatcgc gtcggttctg gataaattca accgcgaaga
     1681 actgcgccgg ctctacccca tgcatatgag ttacggcagg gtggatgcac gcatccgaac
     1741 ctggtacaat ccaaatcttg acagtcagta tttttttgtt cccggcatcg tcgcttttct
     1801 gatcatgttg acgagtttta tctttacgtc catcgccatc atccgggaga aggaagacgg
     1861 caccatggag caattgattg tgacccccat aaaatcacac gaatttatta tcggcaaaac
     1921 cattccctat acgatcattg cgctttttca gttgtttgcc gtaaccggtg tcgccgttta
     1981 ctggtttgag attcccttta aaggaagttt cctgcttctt ttgctggccg cctgtttgtt
     2041 tttgctgagc acgctgggca tcggactctt catctccacc atatcctcga ccaaacagca
     2101 ggccatgatg acggctttct ttttcgtgct gccgttcttc atgctcagcg gtttcgtctt
     2161 tcccatttcc aacatgcctg acgtcgtaca gtggcttacg tatcttaatc cgctgagatt
     2221 ttttctggtc atcatcaggg atatttttct gaaaggcgtt ggctttgatg tgctctggcc
     2281 gcaatacctc gccctgacca tcctgggggc agttgttttt accggcgcca tcgcccggtc
     2341 taaaaaacgg ctggattagt gaagcgttct catatattat
//
LOCUS       DAONQZ010000160         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_46514_length_2347_cov_12.804974, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000160 DAONQZ010000000
VERSION     DAONQZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46514_length_2347_cov_12.804974"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2347
                     /locus_tag="PLR13_12230"
     CDS             <1..>2347
                     /locus_tag="PLR13_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQC20152.1"
                     /translation="SPAQQQAIQGELGKTGGKVTPQAVDALKGRPEFQGVSPAEIEKG
                     KQMLEQKEKAAEKAPEKPIEKASDDAKEKGAEKIEKKNLPWEEKTIIGEEAVENGLFD
                     RSRRVGKYQDVSLSLQLFGSDFFRESAVRVVTDRKDIPVPLKYVIGPGDEVKLLLWGR
                     LNAQYNLTVDRDGKITIPQVGPMFVAGMTFEEMSKHLITQAEQIVGTNIDVSMGALKT
                     ISVFVLGDVRKPGAYTIGSFATITDALVMVGGASNIGSMRRVQLRRNNKLVTTFDLYD
                     LLLKGDKSKDVILQSGDVIFVPVAGPLVGIAGNVKRPAIYELKDKHDLHTVIENAGGI
                     IPTAYTQQIQVERIIKGEKHVVVDINDKNLEHASQFQLQDADLVKIFSIVDANVNAVY
                     INGNVKRPGKYEYKPGMTIRDILSRPDELLPETHYDYALIRRLTPPSMEAALVPFNLG
                     KLIFDHDPAQNIALMPQDQIYIFSKWFFQEKPHFSISGEVRKGGRFDLPDNFTVKDAI
                     LAAGDLTKEAYLKKGEIVRVNQQREYKTIYFNVANALAGDVGDNVLLKDEDRIVIHSI
                     WEERWREVVSVVGEIKNPLQTQLTESMRVSDLVFKAGGVTRDTYYNRAELYRTDWRTK
                     EVTLREIDLGKALAKDPENNLLLKDLDRLVVHSVREKIFEKTVSIDGDVHKPGTYRLA
                     EKMTVRDLVFAAGNVTEAVYLDDAEIASMRVEDGKTAKISVRNINLRKALAGDTAHNV
                     ELHPYDRVFVKRIVDWRQERYVTLAGQVKFPGRYMLSKGERL"
BASE COUNT          605 a          527 c          679 g          536 t
ORIGIN      
        1 tttctcctgc gcagcagcag gccatccagg gcgaactggg aaaaacgggc ggcaaggtca
       61 cgccgcaagc ggtcgatgct ttgaaaggcc gtccggaatt ccagggtgtt tctcctgccg
      121 aaatcgaaaa aggcaagcag atgctggaac agaaggaaaa agccgcggaa aaagcgccgg
      181 aaaaaccgat tgaaaaggct tcggatgacg ctaaggaaaa gggcgcagaa aagattgaaa
      241 agaaaaatct gccctgggaa gaaaaaacga tcatcggcga ggaagcggtg gaaaacggtc
      301 tgtttgatcg ttcacgccgg gttggcaaat atcaggatgt ttctcttagt ttgcaactgt
      361 ttggatctga tttctttcgg gaatcggcgg ttcgtgtagt gaccgaccgc aaggacattc
      421 ccgttccgtt gaagtatgta atcgggccgg gggatgaggt caaacttttg ctatgggggc
      481 gcctcaacgc ccagtacaac ctgacggtgg atcgggacgg caagatcacg attccgcagg
      541 tggggccgat gttcgtcgcc ggaatgactt ttgaggagat gtccaagcat ctgatcacgc
      601 aggccgagca gattgtgggg acgaatatcg acgtttccat gggcgcgctg aagacgattt
      661 ccgtctttgt gctgggtgat gtccgcaaac cgggcgctta cacgatcggc tctttcgcta
      721 cgattacgga cgcgctggtg atggtgggcg gagcgtccaa tatcggatcg atgcggcgtg
      781 ttcagctgcg gcgcaacaac aagctggtaa ccacgttcga cctctacgat cttttgctga
      841 aaggcgacaa gtccaaggac gtcatcctcc agtccgggga cgtgattttt gtgccggtag
      901 ccggccctct cgtcggcatt gccggcaatg tgaaacggcc ggcgatttat gagctcaaag
      961 acaaacatga tctccatacg gtgattgaaa acgcgggggg aattatcccc actgcttaca
     1021 cgcagcaaat tcaggtggag agaattatca agggtgaaaa gcatgttgtc gtggatatta
     1081 acgacaaaaa tctggaacat gcgtctcaat ttcagcttca ggacgcagat ctggtgaaaa
     1141 ttttctccat tgttgacgct aatgtcaatg ccgtttatat caacggcaat gtgaaaaggc
     1201 cgggcaaata cgaatacaaa ccgggtatga cgatcaggga tatactttcc cggccggatg
     1261 aattgctgcc ggagacgcat tatgactacg cgctgattcg gcgtctgacg ccgccttcga
     1321 tggaggctgc gctggtgccc ttcaatctgg gaaaattaat tttcgatcat gatcccgcgc
     1381 agaatatcgc tttgatgccc caggatcaga tatatatatt cagtaaatgg ttttttcaag
     1441 aaaagcctca cttttccatt agcggcgagg tgcgcaaggg cggacgtttt gatctgccgg
     1501 ataattttac cgtcaaagac gctattttgg ccgccggcga tctgaccaag gaagcctatt
     1561 tgaaaaaggg agaaattgtt cgcgtcaatc aacagagaga atacaagacg atttatttca
     1621 acgtggccaa tgctctggcg ggtgatgtcg gcgataatgt tctgctgaag gatgaagacc
     1681 ggattgtgat ccattccatc tgggaggaaa gatggaggga agttgtttcc gttgttggag
     1741 aaatcaagaa tcctctccaa acacagctta cggagtcgat gcgcgtgtcg gatcttgttt
     1801 tcaaggcagg cggtgttacc cgcgacacgt attacaatcg cgccgagctg tatcgcaccg
     1861 actggcgcac aaaagaagta acgttgcggg aaattgatct gggcaaggcc ctggctaaag
     1921 atcctgaaaa taatttgctt ttaaaggatc tggatcgcct ggtcgttcat tccgttcggg
     1981 aaaagatttt tgaaaaaacg gtcagtattg acggcgatgt tcataagccg ggaacgtatc
     2041 ggctggcgga aaaaatgacg gttagagacc tggtttttgc cgccggaaac gtcacggaag
     2101 cggtttatct ggacgacgcg gaaatagctt ccatgcgggt ggaagacggc aagaccgcca
     2161 aaatcagcgt caggaatatc aatctgcgca aggcgctggc cggagataca gcgcataacg
     2221 tcgagctcca tccctatgac cgcgtttttg tcaagagaat agttgactgg cgtcaggagc
     2281 gttatgtgac gctcgcaggc caggtgaagt ttcccggccg ctatatgctg tcgaagggcg
     2341 aaaggct
//
LOCUS       DAONQZ010000161         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_48452_length_2281_cov_13.200359, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000161 DAONQZ010000000
VERSION     DAONQZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48452_length_2281_cov_13.200359"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLR13_12235"
     CDS             complement(<1..234)
                     /locus_tag="PLR13_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20153.1"
                     /translation="MINKFNFNTDKKKLIIYILLVAVTFAAFVQINKHNFVDLDDFVY
                     VTENVHVQSGITLASSRWALTTTYAEFWHPLTWL"
     gene            complement(332..2134)
                     /locus_tag="PLR13_12240"
     CDS             complement(332..2134)
                     /locus_tag="PLR13_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQC20154.1"
                     /translation="MNKKYLYLILFFLIVASLVAFGRIMSNNFINFDDPGYITKNYIV
                     QSGLNPQSIQWAFSTTYLSNWHPLTWLSHMLDWTLFGNNAGGHHIVSLLLHIGTIIFL
                     FLFLNKTTKNIWAAAFAAAFFALHPLRVESVAWASERKDVLSMFFGIACLYAYAFYVE
                     RQKRFRYFLCLILFLFSMMSKPMMVTLPFVLLLLDYWPLQRLKITACARGEFLKTGLR
                     LIVEKIPFLLTTVAVSIVTIWAQAREGAVASSDALPFLTRLSGTVVAYVTYLIKIFWP
                     YQLTVFYPYQFSIPEWQIWMSAITLVLITFTVAYRIRKTPFLFAGWFWYLGTLVPVIG
                     LVQVGKQAIADRYTYLPSIGISIMLAWSLPLLFKNRKFRKRILLPAAIILLIFLSILT
                     WKQCGYWKSSMSLFNHASQVMRHFNPETKNIGSDFTQLGRYQSQQEIQNYDKLIRSKP
                     DDYLTYFNRGIAFAKVGQYQNAIRDYNTALRLKPDYVEAYNNRGNIYGQHGEYKLAID
                     DFTKVIDLNPRHFKAYHNRGLAYSELKRYPEAVKDFDKAIDLKPDYVSAFHNRANVYL
                     KQGNNISGCRDARKACELGNCGLMNSLQKKGLCR"
BASE COUNT          663 a          479 c          527 g          612 t
ORIGIN      
        1 cagccaggtc aaaggatgcc agaattccgc gtaggtagtc gtcaatgccc agcggctgct
       61 cgccagagtg atgccggact ggacatgaac gttctcagtg acataaacaa aatcatcgag
      121 atcaacaaag ttatgtttat ttatctgcac aaaagcagcg aaggttaccg caaccagcaa
      181 gatataaata ataagtttct ttttatctgt gttaaaatta aatttattta tcattggctg
      241 tacccattac tgaaatagca ttctgattca atcttcaaaa catttgttta tcataacaaa
      301 gtcaaatcat tccggtcaaa tgaagcctga atcagcggca tagtcctttt ttttgtaaag
      361 aattcattaa accgcagttg cctaattcgc aggctttgcg tgcgtcgcga cagccggaaa
      421 tattgttccc ctgtttcaga taaacatttg cccggttatg gaaggcgctg acataatccg
      481 gtttcaggtc aatggccttg tcaaagtcct tgacagcttc cgggtagcgt tttagctcgc
      541 tgtaggcaag gcctctgtta tgataggctt tgaaatgacg gggatttaag tctatgacct
      601 tcgtaaaatc gtctatcgcc agtttgtatt cgccgtgttg accgtaaatg ttccctcgat
      661 tattataggc ttccacgtaa tccggtttca gacggagggc cgtgttgtaa tccctgatgg
      721 cgttttgata ctgaccgact tttgcaaaag cgattccccg gttgaaatag gtcagataat
      781 catccggttt tgatcggatt aatttgtcat agttttgtat ttcctgttga gactggtacc
      841 ggcccagttg tgtaaaatca gaaccaatat tttttgtttc cggattaaag tgcctcatga
      901 cctgcgaggc atggttgaaa agggacatgc tgcttttcca gtaaccgcat tgtttccagg
      961 tcaggattga caaaaagata agcaggatga tggccgccgg aagtaaaatt cttttccgaa
     1021 actttctatt cttaaataaa agagggaggc tccaagccag catgatggag atgcctatgg
     1081 acggcaaata cgtgtagcgg tcggctatcg cctgtttccc cacctgaacc agaccgataa
     1141 cgggcacgag cgttcccaga taccagaacc atcccgcaaa taaaaaaggc gtttttctta
     1201 tacgatacgc aacggtgaag gtgatcagaa ccagggtgat tgctgacatc caaatctgcc
     1261 attcgggaat tgagaattga taagggtaaa agaccgtcag ctgataaggc cagaatattt
     1321 ttatcaggta tgtaacgtaa gcaacgactg tgccggaaag ccgtgtgaga aaaggcaatg
     1381 catctgatga agcgaccgca ccttccctgg cttgtgccca gatggttaca atgcttacag
     1441 caacggtcgt gagcaaaaaa gggatttttt ctacaatgag cctgagaccc gtttttagga
     1501 attcgccgcg agcacatgcc gtgattttca agcgctgcag cggccagtaa tccaaaagca
     1561 gcaaaacgaa cggcagggtg accatcatcg gtttggacat catgctgaat aagaataaaa
     1621 tcaggcagag aaaatatcgg aatcgtttct ggcgttcgac atagaaagca tatgcataaa
     1681 ggcaggcgat gccgaaaaac atgctgagga catctttgcg ttccgaagcc caggccaccg
     1741 attccactct caggggatga agggcaaaaa aggcggcggc aaaagccgca gcccagatat
     1801 tttttgttgt tttattcaaa aaaagaaata aaaatataat cgttccgata tgcaaaagca
     1861 gacttactat gtgatgtccg ccggcgttgt ttccgaagag agtccagtcc agcatatgcg
     1921 acagccaggt caacggatgc cagttactta aatatgttgt ggaaaaggcc cattgaatgc
     1981 tttgcggatt cagtcccgac tgaacaatat agttcttcgt tatatagccg gggtcatcga
     2041 aattaataaa attgttgctc atgatgcggc cgaaagcaac cagagaagca acaattaaaa
     2101 aaaataaaat gaggtaaaga tattttttat tcatggggtt tgctgtatct gtttttgcat
     2161 aaaatttgaa aaattgtaaa ccatcgtcat taccggcaca aatttttcga ttttgccagt
     2221 tgcagtgtcg agcaggttcc catattgcag gcttgatgag cgtcccgaca gcctgcttcc
     2281 a
//
LOCUS       DAONQZ010000162         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_50074_length_2225_cov_14.023041, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000162 DAONQZ010000000
VERSION     DAONQZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50074_length_2225_cov_14.023041"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLR13_12245"
     CDS             <1..524
                     /locus_tag="PLR13_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQC20155.1"
                     /translation="QIKALLPDVSIVPLRGNIETRIKKIETENINGVILAAAGMKRNG
                     LTEKITQFLPVETMLPAVGQGALGLQIRKNDDELAKACAVLNDAATSAEVAAERAFLR
                     TLGGGCRLPIAALGKLDGQMLTLEGMLAAPNGTTVIREKISGTIKDAEEMGKKLAEIV
                     LDKGGRRLMDLMC"
     gene            624..2177
                     /gene="cobA"
                     /locus_tag="PLR13_12250"
     CDS             624..2177
                     /gene="cobA"
                     /locus_tag="PLR13_12250"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQC20156.1"
                     /translation="MKKNKKGKVYIIGAGPGDAGLMTLKGIDCLREADVVIYDYLVGK
                     DLLKYARSDARFIYAGKQGGAHTLSQWQINDLLVKEANAGNIVARLKGGDPFIFGRGG
                     EEAEKLAANKIPFEIVPGVTSAIAVPAYAGIPLTHRGLTSTVAFVTGHEDPTKGKSDI
                     DWQALARIGTLVFLMGVKNIEKIVKELEDNGRSPKAPAALIRWGTTARQEILTGTLSS
                     IVKLAREQKFAPPAIFVVGEVVALRDTLQWFDSKPIFGKGVVITRPEKQADDLAQLLI
                     KAGANPIHFPTIKIVPPASWREMDNAIKKLEDYDWLIFTSANGVAYFFERLSAKGKEI
                     RDLKGIKICCIGPATARQVQDKGIRVDLVPKEFIAEGILKSFSRTNLKGKKILIARAA
                     KARDVLPEGLKKLGAQVDVVTAYETVGSGKKKKELEELFKENQVDVIAFTSSSTVHNF
                     IKIMGGGFKLPKGVKIACIGPVTAAAARKAGFPVDIHQEEYTIEGLVDALANYFKKKP
                     AAGKRGKGK"
BASE COUNT          626 a          474 c          617 g          508 t
ORIGIN      
        1 cgcagatcaa ggcgttgctg cctgatgtga gtattgtgcc gctcaggggc aatattgaaa
       61 ccagaattaa aaaaattgaa acggaaaata tcaacggcgt aattttagcg gcagcgggga
      121 tgaaaaggaa cggactgact gaaaagataa cgcagtttct gccggtggaa acgatgctcc
      181 cggcggtagg ccagggcgca ctggggttgc agatacgcaa aaatgatgat gaactggcaa
      241 aagcctgtgc cgtattgaat gatgcggcga cctcggcgga agttgccgcc gaacgcgcat
      301 ttctgcgcac gctgggcggc ggatgtcgcc tgccgatcgc ggcgctgggc aaactcgacg
      361 gtcagatgct gacacttgag ggcatgcttg ccgcgcctaa cggcaccact gttattcgcg
      421 aaaaaataag cggcaccata aaagatgccg aagagatggg caaaaaactg gctgaaatcg
      481 ttctggacaa aggcggccga agactgatgg atttgatgtg ctgatattaa tatgtcattt
      541 cgaagcgaag cgggaaatct tgtttttata aaattggttt tttaagattc ctcacatacg
      601 ttcggaatga caaatggaca attatgaaaa agaataaaaa aggaaaagtt tatattattg
      661 gcgcggggcc gggcgatgca gggctgatga cgctcaaggg tattgattgc ctacgggaag
      721 cggatgtcgt catttacgat tatctggtcg gcaaagattt attaaaatat gctcggagcg
      781 atgcccgttt tatttacgca ggcaaacagg gcggcgcgca tactctgtcg caatggcaaa
      841 tcaacgatct tctggtgaaa gaagcgaatg ccggaaacat cgttgcccgt cttaagggcg
      901 gcgatccatt tatcttcggc cgtggtggag aagaggcgga aaaactggcc gctaacaaga
      961 ttccttttga aattgttccc ggtgtcacat ccgcgattgc cgttcccgct tatgccggta
     1021 ttccgctgac gcaccgcggg ttgacctcca cggtggcttt tgtcaccggc catgaagacc
     1081 cgaccaaggg aaaaagcgat atagactggc aggcactggc ccgaatcgga actttagtat
     1141 ttctgatggg cgtaaagaat atcgaaaaga ttgttaagga attagaggat aacggcaggt
     1201 ctcctaaagc acccgccgcg ctgattcgct ggggcacgac ggcaagacag gaaattctca
     1261 ccggaacttt gtccagcatt gtcaaactgg ccagagagca aaaattcgcg ccacccgcta
     1321 tttttgttgt cggtgaggtg gttgctctcc gtgacacttt gcagtggttt gattccaaac
     1381 cgattttcgg caaaggtgtt gtgattacaa ggcccgagaa gcaggccgat gatctggcgc
     1441 aactgctgat caaagcagga gcaaacccca ttcattttcc gaccatcaag attgttccac
     1501 cggcaagctg gcgcgaaatg gataacgcaa tcaaaaagct ggaggattac gactggctga
     1561 tcttcaccag tgccaacggc gtcgcttatt tttttgagag actttcagct aaaggaaaag
     1621 aaattcgcga tttgaagggt attaaaattt gctgcatcgg tccggcaacg gccaggcagg
     1681 ttcaggacaa gggaatccgg gttgatcttg ttcccaagga attcattgcc gaaggaatcc
     1741 tgaaatcgtt ttcccgcaca aatctgaaag gaaagaaaat ccttattgca cgggcagcca
     1801 aagcgcgtga tgttctcccc gaagggttga aaaaactggg cgcccaggta gatgttgtca
     1861 ccgcctatga aacggtcggt tcggggaaaa agaaaaaaga actggaggaa ctctttaaag
     1921 aaaatcaggt ggatgtgatt gcctttacaa gttcatcaac tgtgcataac tttattaaaa
     1981 taatgggagg cggttttaag ctgcccaagg gagtaaaaat cgcctgtatt ggaccggtga
     2041 cggcagcggc agcccggaag gcaggtttcc ctgttgatat tcatcaggaa gaatatacaa
     2101 tcgaggggtt agtggatgcc ctagccaatt attttaagaa aaaaccggcg gcaggaaaaa
     2161 gaggcaaagg taaatgatag gaatctccaa attatattgc ggggctgtgg aaccgtccga
     2221 tgttt
//
LOCUS       DAONQZ010000163         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_51014_length_2194_cov_18.367929, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000163 DAONQZ010000000
VERSION     DAONQZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51014_length_2194_cov_18.367929"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1130)
                     /locus_tag="PLR13_12255"
     CDS             complement(177..1130)
                     /locus_tag="PLR13_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HQC20157.1"
                     /translation="MNCLCCGKPLHTDNNFGWHKRCIKRFFGTSKLPKIELDEAALEL
                     LATQSTKKGFTIPGVQKKLSLQLQRGQGTTPRLTLINYFAEYIIKPQVEQFKALPESE
                     HLVMRMAHAVGIATVPHALIQSKNSYAYITKRIDRVDGKDGAAKLAMEDFCQLGQRLT
                     EHKYQGSYERCCKIIEKYSDRSKLDLTEFFIRLVFFYITGNSDMHLKNFSLIETFVGS
                     KRYVLSAAYDLLPVKVNIPADKEDFALAMNGKKTKIRKNDFFKFAESVGISKDIAEKL
                     IKQLVDKKNMLQSMCMESLLPNDMKQAFSDLIEKRCLILMS"
     gene            complement(1127..1456)
                     /locus_tag="PLR13_12260"
     CDS             complement(1127..1456)
                     /locus_tag="PLR13_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HQC20158.1"
                     /translation="MSAFRKARVYVRDDFAGLLAETDDGYSFSYDFDYLQSENAAPVS
                     LTLPLQRESFISKTLFSFFDGLIPEGWLFYVVTSNWKIPEKDRFGVLLVACKDPIGNV
                     SIMEEGV"
     gene            complement(1456..1659)
                     /locus_tag="PLR13_12265"
     CDS             complement(1456..1659)
                     /locus_tag="PLR13_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQC20159.1"
                     /translation="MNKIGTYLKKQRKEAGLTQKEFAIRSGLGLRFIRELEQGKKSVR
                     LDKVNVALSMFDMEAVPGKKEKL"
BASE COUNT          663 a          482 c          369 g          680 t
ORIGIN      
        1 ctaggtcatc ttttcctttc tgagatatgt tacttagata gtctcaatgg taaaattggc
       61 tacgctaaca tttgtgtcta cccatattga gacggtggtc tcattagttt taaaaacccg
      121 atagaatcta taaattcaca agttaaattc agattaaacc ccacctataa aatctattaa
      181 ctcatcaata taagacaacg tttttcaatc aagtcgctaa aggcttgttt catatcattt
      241 gggaggagag attccataca cattgattga agcatatttt ttttatcaac tagctgttta
      301 atcaattttt cagcgatatc tttgcttatt ccgacactct cagcaaactt aaagaaatcg
      361 ttcttgcgaa ttttcgtttt tttgccgttc atcgccaagg caaagtcttc tttgtctgcc
      421 ggtatgttga ctttgacagg aagaagatca taggccgcgg acaagacata tcgtttactt
      481 cctacgaatg tttcaatcaa tgagaaattt ttgagatgca tgtctgaatt cccggtgatg
      541 tagaagaaaa cgaggcgaat aaaaaattca gtcaaatcaa gtttgctacg gtcagaatac
      601 ttttcaatga ttttgcaaca ccgttcatat gaaccctgat atttatgttc tgtcaatctt
      661 tgacccaact gacaaaaatc ctccatcgcc agttttgctg caccgtcttt tccgtcaaca
      721 cggtcgatcc tttttgtaat atacgcatac gagtttttgg attgaatcaa ggcatgggga
      781 accgtggcaa ttccgacagc gtgcgccatg cgcatcacca gatgttcgga ttctggcaat
      841 gctttaaatt gttccacttg aggctttatg atatactcag caaaatagtt aataagcgta
      901 agtctgggcg tggtgccttg tcctctttgc aactgcaaag acaatttctt ttgcacacca
      961 gggatggtga atcccttttt ggtgctttgg gtggcaagca gttctaatgc ggcttcatct
     1021 aactcgattt ttggtaattt tgatgttcca aaaaatcttt tgatgcaccg cttgtgccag
     1081 ccgaaattgt tgtctgtatg taaaggtttt ccacagcata aacaattcat acaccttcct
     1141 ccatgatact gacattgcca atcggatctt tgcacgcaac gagcagtacg ccaaaacgat
     1201 ctttttcggg aatcttccaa ttgcttgtca caacatagaa aagccatccc tccggaatca
     1261 atccgtcaaa aaaagaaaat agtgtcttgg aaatgaaact ttccctttgg agcggcaacg
     1321 tcagcgacac cggcgcagca ttttcacttt gaagataatc gaaatcataa gaaaaagaat
     1381 atccgtcatc ggtttctgca agcaatccgg cgaaatcatc cctaacatag acgcgcgctt
     1441 tcctaaaagc actcattaca atttctcctt cttgccgggt acggcctcca tatcaaacat
     1501 tgaaagtgcc acattcactt tgtccaagcg caccgatttc tttccttgtt ctaattctcg
     1561 aataaagcga agacctagac ccgagcgaat agcaaactct ttttgtgtca gtcccgcttc
     1621 tttacgttgc ttcttgagat atgtgcctat cttgttcata caatttcctt ttaacagtag
     1681 tttataccat tatattacac cctttcggga taattgcaat gtaatataac ggaaataaac
     1741 cctataaggt gtaatctgct tgaaattctt tatcaatata ccataaaggg tataattaaa
     1801 catacctatc atcaagaatt aatgataaga aataaatacg gattgagctg ttggatgata
     1861 cacggcacca caactatcgg catattaccc gcttcggatg aaccacgtgg aaatcatcgt
     1921 tagtttacaa ctgaaaaaca taattaatta taaatacaca acctgatttc ctctaaaaca
     1981 cacagaaatc attcctctct aacctcgccc ataacctata tattaaaacc attaaaacga
     2041 tctattgaac cttggtcgtt ttttattatt ccttcgcccg ccccctatat aacaaataac
     2101 aatggaaatt agtaaccaaa cactctgcca aaccgcatac gttagatgac aaactcaatg
     2161 caacataatg cctttatttc gattgattcg gaaa
//
LOCUS       DAONQZ010000164         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_52644_length_2145_cov_14.499522, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000164 DAONQZ010000000
VERSION     DAONQZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52644_length_2145_cov_14.499522"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..713)
                     /locus_tag="PLR13_12270"
     CDS             complement(423..713)
                     /locus_tag="PLR13_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQC20160.1"
                     /translation="MRILFTPTGRRQFLEAIAYIHKDKPSAALSFRQKAEKYLSRLKK
                     FPESGRSLPEFPDLPFREVVVKPYRFFYKVKDNNVWVVAVWHGAQLPDEPAN"
     gene            complement(710..991)
                     /locus_tag="PLR13_12275"
     CDS             complement(710..991)
                     /locus_tag="PLR13_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQC20161.1"
                     /translation="MPNAPKIIPISDLRQNASDVVKGVSSSREPVFITQRGRAAAVLV
                     SMEVYTNAQREMDILHLLARGEKEIEAGTGYALDDVLKEADLYLGDAKR"
     gene            complement(1074..1409)
                     /locus_tag="PLR13_12280"
     CDS             complement(1074..1409)
                     /locus_tag="PLR13_12280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQC20162.1"
                     /translation="MARILRGDIRWADLNPVRGHEQAGLRPVLILSHDIFNERSGTVI
                     AVAITSQPQKAGFPLTLELKSSNLPKKSWLKISQIRTLSVERIGKVIGKASLEELNQV
                     IEGLNEIIA"
     gene            complement(1394..1636)
                     /locus_tag="PLR13_12285"
     CDS             complement(1394..1636)
                     /locus_tag="PLR13_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HQC20163.1"
                     /translation="MGKTKIAITMDAEFIGELDRLVEKNYFQNRSQAICDAVREKLVR
                     MKRSRLSLECAKLDPKFEKAMAEEGLAEDVSLWPEY"
     gene            complement(1701..2081)
                     /locus_tag="PLR13_12290"
     CDS             complement(1701..2081)
                     /locus_tag="PLR13_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20164.1"
                     /translation="MMWLYIKEGFYSIVHKPPCAEDELMVRVRCRRDVDKLQKMLRAE
                     YAFSGDVIESPDADYAYRMIVPRKTLATFLANVAMVMDYDNFKKTIGRLDYRRREAYL
                     RCYDAMYEWQRDIKRGQKSSLTVR"
BASE COUNT          568 a          504 c          427 g          646 t
ORIGIN      
        1 gacgacaaac gtgcgtcgca ttataatgag actccaatat gacaagcgca gcgcagtcag
       61 attggttagt cgaattatcc ccgaggcgaa taacctaaag gtcactatag gccgagcggg
      121 ataaacgtgg ggctaattcc ccgttccttg cggcgccatt cctatggata catcgctgct
      181 gtcatggttg ttcatttttt tccgatgagg aaatttatga attaaatcag tggttaacga
      241 ctggcccatg cgtgatttta aaaaggccag tgcggtgaat cattgacagc tctgaccgta
      301 atggagtgaa atataagcaa aacggttttt tgttgtcgtt aaatttaagg attattactt
      361 gaaaggttta acgaaaagat cagtcggtgc acagctatag gctgtattga ttttattaac
      421 cattaatttg ctggttcatc aggtaactga gcaccatgcc ataccgcaac gacccataca
      481 ttattatcct taaccttata gaaaaaacga tagggcttta caacgacctc acgaaaagga
      541 agatctggaa actcaggtag cgaccttccc gactcaggaa actttttgag ccgggagaga
      601 tatttctctg ctttttggcg aaaagataat gcagcagaag gcttgtcttt gtgtatatac
      661 gcaatcgctt caagaaattg acggcggcct gtgggcgtga ataatattct caacgttttg
      721 catccccaag ataaagatcg gcttctttta acacatcatc gagtgcatag ccggttcctg
      781 cctctatttc tttttcgcct ctcgcgagca agtgtaaaat atccatttca cgctgcgcgt
      841 ttgtatatac ctccatgctt accagtacag cggccgctct tccacgctgt gtaataaaaa
      901 ccggttctct tgaagatgaa acacccttca ctacatcgct ggcattttgc ctaagatcgg
      961 aaatgggtat aatttttggt gcatttggca tttggttgta cctcctattg tatttttaat
     1021 tgtacaacca atagacgctt ttgtcaagat taacggcaat attaccggag ttgtcacgca
     1081 ataatttcat tcagtccttc aatgacctga ttcaactctt ccagagatgc cttgccaatg
     1141 acttttccta ttctttctac agacagggta cgaatttgac taattttaag ccatgatttt
     1201 ttgggtaaat ttgaagattt caattcaagg gttaatggaa atccagcttt ctgtggttgg
     1261 cttgtgatag ccacggcgat gacagtacct gagcgttcat taaaaatgtc gtggctcaga
     1321 atgagcacag gacgcaagcc tgcctgttca tgaccccgca caggattcaa atccgcccac
     1381 cgaatgtcac ctctcaatat tctggccata ggctcacatc ctccgctaat ccttcttccg
     1441 ccatagcttt ttcaaatttc ggatcgagtt ttgcgcattc caaagatagc cgactgcgct
     1501 tcattctgac gagtttttcg cgcacagcat cacagattgc ctggctgcga ttttggaagt
     1561 aattcttctc gaccagacga tccaattcac cgataaattc ggcatccatt gtaattgcta
     1621 tttttgtttt tcccataatt agcctcccct gaaggtattc tattatatca taccattgaa
     1681 aaaagaagca aatataattt ttatcggact gtcaacgaac ttttctgtcc cctttttata
     1741 tccctctgcc attcatacat ggcatcataa caccgcagat aagcttcacg cctccgataa
     1801 tccaggcggc cgatggtctt tttaaaattg tcataatcca tgaccatggc aacattggcc
     1861 aggaatgtgg ccagtgtttt tctagggaca atcatcctgt aggcgtaatc ggcatccggc
     1921 gattcgatga catcaccgct aaatgcatat tccgccctca acattttctg cagtttatcc
     1981 acatcacgcc tgcaccgcac gcgaaccatc agttcatctt ccgcgcacgg cggtttatgc
     2041 acgatggaat agaaaccttc tttaatatac agccacatca ttttcttccc ctttcgtgaa
     2101 ttctacgtga ttgacattac ccggttgttt ctgatacggc aatgt
//
LOCUS       DAONQZ010000165         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_56585_length_2034_cov_7.661950, whole genome shotgun sequence.
ACCESSION   DAONQZ010000165 DAONQZ010000000
VERSION     DAONQZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56585_length_2034_cov_7.661950"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLR13_12295"
     CDS             complement(<1..273)
                     /locus_tag="PLR13_12295"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQC20165.1"
                     /translation="MKEKEMEIRYDMTGVDWKDVAQTLKSVGMSYHEPDEHRRAFAAS
                     HTTVFAYRGDRMIGFGRALADGVYQAALYDIAVVPEFQGQGVGTLIV"
     gene            582..>2034
                     /locus_tag="PLR13_12300"
     CDS             582..>2034
                     /locus_tag="PLR13_12300"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQC20166.1"
                     /translation="MRKIEDQNQEFKQVWNDNCLKTICAFANTTGGKLYIGIDDEGNA
                     TGVKDAARYLDDIPNKTKDILGITPAVKIKKKAGKEIIEISVEPSSAPISYHGRFFTR
                     SGSSSIEIKGHELVEMLMRKSGRSWDGFIEEDATLKDIDTAAVNKFRKLATKKIPVIA
                     KEKNVTAILQKLNLLKRGKLRRAALLLFGKNPKKFYITAYIKIGKFISSTDLISTDDI
                     EGNIFDQVDRAIEMLRVKYLLSNITYEGIYRKDNMEYPEEALREAIINAVIHRNYMGA
                     HTQLRIDPDSLNLWNEGTLPPDIKIDDLKKWHLSRPRNEFLADAFFKAGMIEAWGRGT
                     IKIVDECKKAGLPEPEFRQEFGGLSVRFRKADKAHERAQVEDQVGTKSKQHRVQIGRQ
                     LESQPESRPESRPESLDIRIIRRLVPTPLSKAELSALLGQKEISGQLNKIIRELLQKK
                     VIEYTIPDKPNSRMQKYLLTDKGRKILVTIQGDK"
BASE COUNT          588 a          520 c          473 g          453 t
ORIGIN      
        1 cacgatcagc gtgccgacgc cctgtccctg aaactcggga acgacggcga tatcgtaaag
       61 ggccgcctgg taaacaccgt ccgcgagagc ccggccgaat ccgatcatcc ggtcgccccg
      121 ataagcaaag accgtcgtgt ggcttgcggc aaatgccctg cggtgttcgt caggctcatg
      181 ataactcatg ccgacgcttt tcaacgtctg agccacatcc ttccaatcca caccggtcat
      241 gtcgtatcgg atttccattt ccttttcttt cacaaaatgt tctcgtgttt gagagcgtac
      301 cacttccctg aagaatatgc aaagatcgcc ctgtgcatta aagaaggagt caaggtctgc
      361 cttagacctt tgatgcccag ttctatttgc tataattctt tctgtaattt gcaatatcaa
      421 aagccgcacc gatttcaacg agctttttcc cgttgtctcg ccccttgaac tttttcacca
      481 tccaccaatt acattttcct tgcattgata taataaaatt gtaagatgtc gaaaatcatg
      541 atgttctcat aataccaatt ggaaacgggc gaagaatcat catgcgcaag attgaggatc
      601 aaaatcagga attcaaacag gtctggaatg ataactgcct gaagaccatt tgcgcgtttg
      661 ccaataccac cggggggaaa ctctacatcg gcattgacga cgaaggaaac gccaccggcg
      721 tgaaagacgc cgcccgctat ctggatgaca tacccaacaa gaccaaagac attttaggca
      781 ttacgcccgc cgtcaagatc aaaaagaaag caggcaaaga gattattgaa atatcggtag
      841 agccgtcttc cgcgccgatt tcgtatcatg gccggttctt tacccgcagc ggcagctcgt
      901 cgattgaaat caagggccat gaactggtgg aaatgctgat gcgcaaatcc ggccgttcct
      961 gggacggatt tattgaagaa gatgcaacgc tcaaagacat cgacaccgcc gccgtcaata
     1021 aattccggaa gctggctact aaaaaaatcc ccgtcattgc aaaagaaaaa aatgttacgg
     1081 ccatcttaca gaaattaaac ctcctgaagc gcggcaaact acgccgcgcc gctcttttgc
     1141 ttttcggcaa aaatccgaag aagttctaca tcaccgccta catcaagatc ggcaagttta
     1201 tcagctcgac cgacctgatc agcacggacg atattgaagg gaatattttt gatcaggtgg
     1261 accgagccat agaaatgttg cgcgtcaaat atctgctttc caacatcacc tatgaaggca
     1321 tttaccgtaa agacaacatg gagtatccag aagaagccct gcgggaagcc atcatcaatg
     1381 cggtgattca ccgtaattac atgggagcgc acacacagct tcggattgac cccgacagcc
     1441 tgaacctctg gaatgaagga acgttgccgc ccgatatcaa aatcgacgat ttgaaaaaat
     1501 ggcatctgtc ccgcccgcgc aatgagtttc tggccgacgc ttttttcaaa gcgggcatga
     1561 ttgaggcctg gggccgcggc acgattaaga tcgttgatga atgcaagaaa gccggactgc
     1621 cggaaccgga attccgccaa gagttcggcg gcctatccgt acgcttccgg aaagcggata
     1681 aggcccatga aagagcccag gtcgaggacc aagtcgggac caagtcaaaa cagcaccggg
     1741 tgcaaattgg aaggcagcta gagtcgcagc cagagtcacg gccagagtca cggccagagt
     1801 ctctagatat caggattatt cgacgccttg ttcctacgcc tttgagcaaa gcagaattgt
     1861 ctgcattgct tgggcagaaa gaaatatccg gccaattaaa caagattatt cgtgaactgt
     1921 tacaaaagaa agtaattgaa tatacgattc ccgataagcc caacagccgc atgcaaaaat
     1981 acctgcttac cgataaaggc cggaagattt tggtcacgat acaaggagat aaaa
//
LOCUS       DAONQZ010000166         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_57325_length_2015_cov_9.899490, whole genome shotgun sequence.
ACCESSION   DAONQZ010000166 DAONQZ010000000
VERSION     DAONQZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57325_length_2015_cov_9.899490"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLR13_12305"
     CDS             complement(<1..1149)
                     /locus_tag="PLR13_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQC20167.1"
                     /translation="MLKLFPPDDPLQSVRIKRFLMAFASYSVWLTIALITYLLGIAPV
                     SFHVLFICFMGILLCNILIYAAIRSGFNKRFDDPSLTLFQMIIATFWAMVILYYTDEA
                     RGAVLIVYLVVFVFGLFKLNLRQFFYLSVFAVLNYALVLFLLYKNHPESLNIENEILG
                     LIVLALVLPWFSFMGGYITRLKKKVTHSLAQTKEMELKFRTIFDSASDGITMLDTRQG
                     TFVAANATLCQMLGYTQEEFLKLKISDLHPKESQKDILSIYKKINHHELSIARNIPLL
                     KKNQTILYADISGSLISLEGKEYSVGVIRDITERKIAEELLIASEQRYRLLADNVTDI
                     IFTMEMNLKLTYLSPSGYRLSGYNEDEIARMNISDMVEPETYFRFAEVL"
     gene            1361..1747
                     /locus_tag="PLR13_12310"
     CDS             1361..1747
                     /locus_tag="PLR13_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20168.1"
                     /translation="MSHKLKKESDDAGIVITFSGMVEADEVDNIQEQINSGADFPQLR
                     YQIWDFSKAEEINISIDQIQDIAMHTALASSKNPKLRIAIIPRKTSHNVLGKTFHTFE
                     KVWGSYESKSFRDIHAAREWGQSGKT"
BASE COUNT          615 a          411 c          481 g          508 t
ORIGIN      
        1 cagaacttcg gcaaaacgga aataagtttc cggttcgacc atgtcggaaa tgttcatccg
       61 ggcgatttcg tcctcgttat accctgaaag acggtagccg gaaggactga ggtaggtgag
      121 tttcaggttc atctccatgg taaaaatgat gtcggttacg ttgtcggcaa gcaggcgata
      181 acgctgctcg cttgcgatca gcaattcctc cgcaatcttg cgctctgtaa tatctctgat
      241 gacacccacg gagtattctt tgccttcaag ggaaataagc gatccgctga tatcggcata
      301 taagatggtt tgattttttt tcaggagagg gatgtttctg gcaatcgata attcatgatg
      361 gttgatcttt ttatagatgc ttaaaatatc tttttgcgat tctttcggat gcagatcgga
      421 tattttcagc ttaagaaatt cttcctgggt atagcccagc atctggcaaa gtgttgcatt
      481 ggcggcaacg aaagttccct gtcgggtatc caacatggta atcccgtcag aagcagaatc
      541 aaagatggtt ctgaatttca gttccatttc tttcgtctgg gcaagggaat gcgttacctt
      601 ctttttcagc cgggtgatgt atccgcccat aaaggaaaac cagggaagaa ccagcgccag
      661 aacgatcagc cctaaaattt cgttttcgat gttcagagat tcggggtggt ttttgtagag
      721 aagaaacaga accaaagcgt aattcaacac ggcaaagacg gataaataaa aaaactggcg
      781 caaattcaac ttgaaaagtc cgaaaacaaa aacgactaga tagacaataa gaacagcgcc
      841 ccgggcttca tcggtataat acaaaataac cattgcccag aacgtggcga tgatcatctg
      901 aaacagtgtc aggctggggt cgtcaaatcg tttattgaag ccggatcgga ttgcagcata
      961 aatcagaata ttgcataaaa gaatacccat gaaacaaata aaaagaacat gaaacgatac
     1021 cggtgcaatg cccagcagat aggtgatcag cgcaatcgtc aaccacacgg aataagaagc
     1081 aaaggccata agaaatcgtt tgattcttac agactggaga ggatcgtcgg gcggaaacag
     1141 tttcaacatg accaatcgcc tcaaagtgat gaattttgta attgattttt tttaccacaa
     1201 aataaaatca agttcaatat atcgctcgat gtatttagaa agaacgatgg atgcgcctga
     1261 cgccttcagg taaaatatac ggtaaaattt tcgtatctgt gctattaaaa aaagggtaac
     1321 agttcgtaca acacttgaca tcaactcggg ggtcatcatc atgtctcata aattgaaaaa
     1381 agaaagtgat gatgccggta tcgtcattac tttttccggc atggtcgagg cggacgaagt
     1441 cgacaacatt caggagcaga tcaattccgg tgcagatttt ccacagttgc gttatcagat
     1501 atgggatttt tccaaggccg aagagatcaa tatttccatc gatcagattc aggatatcgc
     1561 catgcacacc gcgctggcat ccagcaaaaa tccgaaactg agaatcgcca tcattccccg
     1621 caagacatcg cataacgttc tggggaaaac ctttcacacg tttgaaaagg tttggggatc
     1681 ctatgaatcg aaaagtttcc gggatattca cgctgctaga gaatggggcc aaagcggcaa
     1741 gacgtaaccg cttgtccggt ccggtggtcc tctgcctgtg tccggaaata gatttgcaga
     1801 tggtcgatat cgcgggcatc gggggataga cacggatatg agccttcccg ggatattatt
     1861 tcgacaggaa cggatcgttc acaggtcata tgtcccgtgg ggaatggata aggagagctt
     1921 aaaatgaagg tcgaatttta ctgctggagc aaatgaacta cctgccggaa agcgaaagag
     1981 tttcttttgc agaaaaaggt ggaaatcaac gaaag
//
LOCUS       DAONQZ010000167         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_58422_length_1987_cov_11.681159, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000167 DAONQZ010000000
VERSION     DAONQZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58422_length_1987_cov_11.681159"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLR13_12315"
     CDS             complement(<1..497)
                     /locus_tag="PLR13_12315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC20169.1"
                     /translation="MHIEEQISRESGAHGKQWHSMHDGYFADAAVARPLIETIIRHVS
                     DSDTDIVVDLGGGTGFILQRLIAAGLSRHIASVNLDCSASQLGAMKENRIACVNRYIS
                     DFTRRDLADENKHISFIMRSVLHYFGQNGLTPVLRHIRRQAKDGEVFIHQTACFENET
                     DAQCI"
     gene            749..1213
                     /locus_tag="PLR13_12320"
     CDS             749..1213
                     /locus_tag="PLR13_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HQC20170.1"
                     /translation="MELKSLSFQHENMIPAQYTCDGRNVSPPLSWSGAPDKTKSFALI
                     CDDPDAPVGLWVHWVLFDIPASVNALPENFSRTEDIPGFGKNGKNTSGRFGYDGPCPP
                     DGTHRYYFKLYALDTMLNLKPGLTKDGLLQAIKGHVLAEAQLMGRYKRPAGR"
     gene            complement(1248..>1987)
                     /locus_tag="PLR13_12325"
     CDS             complement(1248..>1987)
                     /locus_tag="PLR13_12325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQC20171.1"
                     /translation="HELSDAFYTTETMQGYAVLKPHGYAGDFVIIERIYQHWLSPKER
                     LINWDHFFHAQDAPRAILNRKKYFLGILKDLEKKDGKKDVLNIGCGPCQDVVEFLSQG
                     PSQVLFECVDYDKNAIEYSYNKVKAKGFLGNIEFYRDNAFKFRPARQYDLIWSAGLFD
                     YLDEKTFKMLLTKQLAFLKEGGEIVLGNFSQNNSTRDYMEFGNWFLHHRSEDELTQIA
                     VDCGVPVSNIDVRSEPLGVNLFLHISK"
BASE COUNT          533 a          486 c          455 g          513 t
ORIGIN      
        1 ttaatgcatt gcgcatccgt ttcattttca aaacatgctg tctgatgaat aaatacctcg
       61 ccatctttgg cctggcggcg aatgtggcgc aggacgggcg ttaagccatt ctgtccgaaa
      121 tagtgtaaaa cagaccgcat aataaaagag atgtgtttgt tctcgtcggc caaatctctg
      181 cgggtaaaat cggagatgta ccggtttaca caggcaatac ggttctcttt catggccccc
      241 agttggcttg cagaacaatc aagatttacc gacgcgatat gtcgggacag tccggcggca
      301 atcagacgct gaagaataaa ccctgttccg cctcccagat ccaccacaat gtctgtatcg
      361 gaatcggaga catgtctgat aatggtttca atcaagggac gcgcaacagc cgcatcggca
      421 aaatatccgt catgcatgga atgccattgt tttccatgag ctccgctctc ccggcttatt
      481 tgttcttcga tatgcatggc gcaccttgtc tcctgattaa ggttcatgat accacaggat
      541 catcaggtgt caacagcgtt cgttggcgct attgacgatt ttaaaatctc cggttaaaat
      601 accggcataa gcttggcaga tcgaaaatgg tgaaacaatg aaaatttatt tcttttgttt
      661 ttctgtgatt atttgtttca ttattggcaa cgccgtcacc attcaagccc atcaacgagg
      721 cagtttcaaa actaaaggag gaaagaccat ggaattaaaa agtctgtctt ttcagcatga
      781 aaatatgatt ccggctcaat atacctgtga cggacggaat gtatctccgc cgctttcgtg
      841 gagcggcgct ccggacaaaa caaaaagttt cgcgctgatc tgcgatgatc ccgacgcacc
      901 ggtcggcttg tgggtgcact gggtcctttt cgacatcccc gcatcggtca atgcccttcc
      961 ggaaaatttt tcccgaacgg aagacattcc cggattcggg aaaaacggga aaaacacgtc
     1021 tggacgtttc gggtatgacg gcccctgccc tccggacggc acacaccgct attattttaa
     1081 attatatgcg ctcgatacga tgctgaatct caagccgggc ctgaccaaag acggcctgct
     1141 ccaggcaatt aaaggacatg ttctggcgga agcacagttg atgggccggt ataaaagacc
     1201 ggcgggcaga taacgccctc attggaaatg caatctgcgg gatttattta cttggaaata
     1261 tgcaggaaaa gattgacgcc cagcggttcc gaccggacat caatattgct gacgggaacc
     1321 ccgcaatcga ccgcaatctg ggttaattca tcttcagagc ggtgatgcag aaaccagttg
     1381 ccaaactcca tataatcgcg ggtggaatta ttctgtgaaa aattccccag aacgatttct
     1441 cccccctcct tgagaaaagc aagctgtttg gtcagcaaca ttttgaaagt tttttcatca
     1501 agataatcaa aaagaccggc cgaccagatc aggtcatact gccgagcggg tctgaatttg
     1561 aaagcattgt cgcgataaaa ctctatattg ccgaggaagc cttttgcttt caccttattg
     1621 taagaatact ctatggcgtt tttatcgtaa tccacacatt caaacaacac ttgagacggc
     1681 ccttgggata aaaattcaac cacgtcctga cagggtccgc acccgatatt cagcacatcc
     1741 tttttcccgt cttttttctc gaggtctttc aggattccaa gaaaatattt tttccgattc
     1801 aaaatggcgc gcggcgcatc ctgagcatga aaaaagtgat cccaattgat gaggcgctct
     1861 ttaggagaca gccagtgctg gtaaattctt tcaatgatca caaaatcacc ggcatacccg
     1921 tgaggcttca ggacggcgta tccctgcatg gtctcggtcg tataaaacgc atcggacaat
     1981 tcgtgcc
//
LOCUS       DAONQZ010000168         1906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_61873_length_1906_cov_35.689357, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000168 DAONQZ010000000
VERSION     DAONQZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61873_length_1906_cov_35.689357"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1052)
                     /locus_tag="PLR13_12330"
     CDS             complement(90..1052)
                     /locus_tag="PLR13_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20172.1"
                     /translation="MMKKLNDKRYLVFSGLAVGVSLLLVAAFNYVMDPYNLLGNNRTG
                     VYFWNERQVKDVILTYKHEGILIGSSKTGYVNPDDLSCYRFYNASMRGMVPEEMYFYL
                     KKYLRREKLVLVGFDFFMFNEREFPLIRIADWDDLRYGRMEYLLSAETVKSSWKTLTK
                     WRKKEKKHGMKANGQFDYPGASEAPASPGSQQQYEKQYQDIIKGLVQNHYYKFSFSQT
                     RIDYVKKIKTLLEENKIPYAVFINPLNHDVLAALQNIDAYSLFLNWKREMKSIFPDIY
                     DYSISRYSAREGFYRVDPFHYTNATGVAFLNEIIHDFCPEGSKP"
     gene            complement(1053..>1906)
                     /locus_tag="PLR13_12335"
     CDS             complement(1053..>1906)
                     /locus_tag="PLR13_12335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQC20173.1"
                     /translation="LAYTLQIYFDFSGYSDMAIGLAFLFGIRIPLNFNSPYQSLNVID
                     FWRRWHVSLSRFLRDYLYIPLGGNRKGEAMRFVNLMITMLLGGLWHGAAWTFVLWGGL
                     HGLFLIINHGWVKIKRRFGYEGKGGRTGRFFSGALTFLAVTVAWVFFRADSAATAFRI
                     IGSMAGQNGFALPEKYLDKFSVLAMPLQAWGVDFHSMHYFKGSNEVVYILIALLVVWL
                     APNVQQIMDLCMPYLNIMAKGKKLAQPPAWLTWRPSYFWAIVLVVCALVSLLSMGRVS
                     EFLYFQF"
BASE COUNT          543 a          481 c          429 g          453 t
ORIGIN      
        1 caggcgaccg gccttgccgc cccgtcaaaa tcaccatcag aaaagagcct tccgattcaa
       61 atctgattta ccggttcagg aaaaaactct tacggctttg acccttccgg acaaaaatca
      121 tggatgatct catttaaaaa cgcgactcct gtggcattgg tgtaatgaaa gggatccacc
      181 cgataaaacc cctcccgcgc tgaataccgg ctgatggagt aatcatagat atcggggaat
      241 atactcttca tctcccgttt ccagttcaag aacagcgagt aagcgtcaat attttgaagc
      301 gccgccagca cgtcgtgatt cagaggattg atgaataccg catagggaat tttattttct
      361 tccagaagcg ttttgatctt tttcacataa tcgatccgtg tttgcgaaaa agaaaacttg
      421 taataatggt tttgtaccag ccctttgatg atatcctgat attgcttttc atattgctgc
      481 tgagatcccg gagaagcggg ggcctccgat gctcccggat agtcgaattg cccgttcgcc
      541 ttcatgccgt gctttttttc ttttttgcgc cacttggtca aggtcttcca tgacgattta
      601 acggtttccg cgcttagcag gtattccatc ctgccatagc gcagatcatc ccagtccgcg
      661 attctgatca gcggaaattc tctttcattg aacatgaaga aatcaaagcc caccaacacc
      721 agtttttcac ggcgcaggta tttctttaaa taaaaataca tctcttccgg aaccattccg
      781 cgcatggaag cgttgtaaaa tcgatagcag gaaaggtcgt cgggattgac atatccggtt
      841 ttgctggacc caatgagaat tccttcatgt ttataggtca atatcacgtc tttgacctga
      901 cgctcgttcc aaaagtaaac gcccgtcctg ttattgccca gcagattgta agggtccata
      961 acataattga atgctgcaac cagcagcaac gagaccccga cggccaggcc cgaaaaaaca
     1021 agatatcttt tatcgtttaa tttcttcatc atttaaaatt gaaaatagag aaattcgctg
     1081 acccggccca tggaaagcaa tgaaaccaga gcgcacacga caagcacaat cgcccagaag
     1141 tatgagggac gccaggtcag ccatgccggc ggttgggcca gtttcttgcc cttggccata
     1201 atatttaaat acggcatgca aagatccatg atttgctgga cattgggcgc cagccagaca
     1261 accagcaggg caatgaggat ataaaccact tcattgctgc ccttaaagta atgcatggaa
     1321 tgaaaatcaa ccccccaggc ctgaagcggc atggccagca cagaaaattt atccagatat
     1381 ttttccggca gggcaaaacc gttttgaccg gccatggaac cgatgatgcg aaacgcagtg
     1441 gcggcgctgt cggccctgaa aaaaacccaa gccaccgtaa cggcaagaaa tgtaagcgct
     1501 ccggagaaga aacggccggt ccgtccgccc tttccttcat aaccaaaccg ccttttgatt
     1561 tttacccatc cgtgattgat gatcagaaat aaaccgtgca acccgcccca caggacaaag
     1621 gtccatgccg cgccgtgcca aagccctccc agcagcatgg tgatcatcaa atttacaaag
     1681 cgcatggctt ctcccttgcg attgccgccc agagggatat agagatagtc ccgtaaaaaa
     1741 cgggaaagcg acacatgcca tcttctccag aaatcgatga cgtttaagga ttgataggga
     1801 gaattaaaat tcaggggaat gcggataccg aacagaaagg ccagaccaat ggccatatcg
     1861 gagtagccgg aaaagtcaaa atagatttgc agggtatagg ccaggg
//
LOCUS       DAONQZ010000169         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_64243_length_1857_cov_15.667592, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000169 DAONQZ010000000
VERSION     DAONQZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64243_length_1857_cov_15.667592"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..562)
                     /locus_tag="PLR13_12340"
     CDS             complement(29..562)
                     /locus_tag="PLR13_12340"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQC20174.1"
                     /translation="MKNEIGLIIKIAIICISCAVLFGCGYSFAPRGESIDTRIQKVYV
                     EPFENNTAQAEIENYVRAAFINQFIQHSRFKLTSGTETADAVVRGKIVNLHTSSLTHL
                     KNDLAAEERATMTMEVVFEDKSDGRIIWSAKNITDSVDYVLNEDINMLPASKKQAFMK
                     LADDMAEKAFNLMMSGF"
     gene            complement(751..>1857)
                     /locus_tag="PLR13_12345"
     CDS             complement(751..>1857)
                     /locus_tag="PLR13_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQC20175.1"
                     /translation="MDTFVESSWYFDRYCCPDYDTKPGLDRKKLDYWMPVDQYIGGIE
                     HAILHLLYSRFYTKVLRDFGVIGVDEPFTNLLTQGMVCKETMKCKEHGYLFPDQAADG
                     KCHLCGKDVIIGKTEKMSKSLKNVVDPDYLVKKYGADTARIFCLFAAPPEKDLEWSDQ
                     GVDGSFRFLGRLWRIVDEYLEDIKGVAPANGALELEGDLKTLRRKTHQTIRKVSIDIE
                     DRFHFNTAISAVMELVNVVYQVKRPASSDKVALSVVREALEAAIILLVPIVPHITEEL
                     WQLLGHQNLASDISWPEFDPAIASEEEMTIVVQINGKLRSRMTVPVDESEEKIKADAL
                     ADEKIKSMTSGMQIKKVIYVPKKLVNIVVTLSES"
BASE COUNT          452 a          486 c          399 g          520 t
ORIGIN      
        1 tcacttcgtt cgtcttcggc ttggctcact aaaatccgga catcatcaga ttgaatgctt
       61 tttccgccat gtcgtcggcc agtttcataa aagcctgttt ttttgacgca ggcagcatgt
      121 taatatcttc atttaaaacg tagtcgaccg agtccgttat atttttcgca ctccagataa
      181 ttctgccgtc ggatttgtct tcaaagacca cttccatggt catggtcgcc cgctcttccg
      241 ccgccagatc gtttttcaga tgtgtaagcg atgaggtgtg aagattgaca atttttcctc
      301 ggacgaccgc atcagccgtc tcggtgcctg aagtcagttt aaaacggctg tgctgtatga
      361 actgattaat gaaggccgcc cggacataat tttctatttc agcctgcgca gtgttatttt
      421 caaagggctc cacgtaaact ttttgaatgc gggtatcgat ggattctccc cgcggagcaa
      481 aggaataacc gcagccgaat aaaacggcgc aggaaataca aattattgcg attttaatta
      541 ttaaaccaat ttcgtttttc atcctaatac ctttaattac aaagcttttg aactgtgtcg
      601 aaatatcttt gctaatttca tttgactgcg ataaatctta atcctgcttc gtttcgggaa
      661 tactttaact tactctttaa tgaaccttgg ctatagtctt aaaacgaatt atcccgccac
      721 cttcaggtgg cagggacgag cgtcgctccg ctagctctca gagagcgtca ctacaatatt
      781 aaccagcttt ttcggcacgt aaattacttt cttgatttgc atgcccgagg tcatagactt
      841 gattttttca tctgccagcg catccgcttt gattttttct tcactctcat ccaccggcac
      901 cgtcatccgg cttctgagct ttccattaat ctgcacgaca atcgtcattt cctcttcgct
      961 ggcaatggcc ggatcaaact cgggccagga tatgtcggat gccagatttt gatgtccgag
     1021 caactgccag agttcttccg taatatgagg gacaatcgga accagcaaaa taatggccgc
     1081 ctccagcgcc tcgcgaacca cagacaaagc aaccttatcg gaacttgcgg gacgtttgac
     1141 ctgataaaca acattcacca gttccatcac ggcgctgata gccgtgttaa aatggaagcg
     1201 gtcttcaata tcaatggaaa ccttgcggat cgtctggtgg gttttgcgcc gtagcgtttt
     1261 caagtcacct tccagttcca acgcgccgtt tgcgggagcc acgcctttga tgtcttccag
     1321 atattcatca acaatacgcc acagtcgtcc cagaaaacgg aaagaaccgt ccaccccctg
     1381 atcgctccat tccagatcct tctccggcgg cgcggcaaac aggcagaata tccgcgccgt
     1441 atcggcaccg tacttcttga ccagataatc ggggtcaaca acgtttttca acgatttgga
     1501 cattttctcc gtcttgccga taataacgtc tttcccgcag aggtggcatt taccatctgc
     1561 cgcttgatca gggaaaagat aaccgtgctc cttgcatttc attgtttcct tgcagaccat
     1621 gccctgcgtc agcagattgg tgaacggttc gtccacaccg atcacgccga aatcgcgcaa
     1681 taccttggta taaaagcgcg agtaaagcag atgcagaatc gcgtgctcga tgcctccgat
     1741 gtactgatcc acgggcatcc agtaatccag ttttttgcgg tcaagaccgg gtttagtgtc
     1801 gtaatccggg cagcagtaac ggtcgaaata ccaggacgac tccacgaatg tatccat
//
LOCUS       DAONQZ010000170         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_65765_length_1826_cov_19.628458, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000170 DAONQZ010000000
VERSION     DAONQZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65765_length_1826_cov_19.628458"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1826)
                     /locus_tag="PLR13_12350"
     CDS             complement(<1..>1826)
                     /locus_tag="PLR13_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQC20176.1"
                     /translation="YYIYEAGGIAHLAPDHEKLIRLGTDEIKKEIEALKLKNKNPDQE
                     SFYHAALLSADALALFGERYAACAEVLAEKEEDAMRSLELHDIAAVCHHVPRGGARTF
                     HEALQSLFFLHVAIFQESMGETVCPGRIDQILYPYYKKDREAHLITRKRAKELLESFC
                     IKLCETIPVYSQAITNLAGGMPSWQVVTMGGQDQEGNDATNELSYLLLEIATELRMRQ
                     PNFHARIHSGSPPEFKKAIYTALAEGSNTPALFNDDVIIPAMTSAGYDIRDARDYVAI
                     GCVEPTAPGKTLGSTDAAILNIPLAVELALNQGKRFGSRRREGVNTLPVAKMKAMDDV
                     TTAFIIQLDYLIGRLISDLKAIEHAHALYRPLPLTSLFIEGCLSSGLCATQGGARYNF
                     SGIQGVGISTAGDSLCAIEKLVFEERKLSLKRLRDLLAARETDPYWFTLMQKAPKFGN
                     NNDEADFWTKFVVDEYARLIRDAGKNTRGGQYLPGIYSNTAHAHFGKMTGALPCGRLR
                     GVTFPSGMAPQNGMDRSGPTALINSMNKIDYKEIANGINFNLKLDANSVRDEKGRHFL
                     NALLDVYFRRGGMQVQLNVLDAGVLREAKEHPEKYPFLLVRI"
BASE COUNT          426 a          495 c          467 g          438 t
ORIGIN      
        1 aaatgcgcac gagcaggaaa ggatattttt caggatgttc tttggcttcg cgcagaacgc
       61 cggcatccag aacattcagt tgaacctgca tgccgccgcg ccggaaatac acatcgagca
      121 gcgcattgag aaaatggcgt cctttttcat cacgcacgga atttgcgtca agcttcagat
      181 taaaattgat gccattggcg atttccttgt aatcaatttt gttcatggaa ttgatcagcg
      241 ccgtcggacc gctccggtcc atgccgtttt gcggcgccat gcccgaagga aaagtcaccc
      301 cgcggagccg tccgcacgga agcgctcccg tcattttgcc gaaatgcgcg tgcgcggtgt
      361 tggagtaaat ccctggcaga tactgaccgc cgcgtgtatt cttcccggca tcacggatta
      421 aacgagcgta ttcgtcgaca acgaattttg tccagaaatc ggcctcgtcg ttgttattcc
      481 cgaatttcgg agctttctgc atcagtgtga accagtaagg atcggtttca cgcgccgcga
      541 gaagatcgcg aagacgcttc agcgatagct tgcgttcctc aaagacaagt ttctcgatcg
      601 cgcacagcga gtcgcccgct gtggaaattc cgacgccctg tatgccggaa aaattgtatc
      661 gcgccccgcc ctgcgtggcg cataaacccg acgacaggca gccttcgata aaaagactgg
      721 tcagcggaag gggacgataa agagcgtggg catgctcaat ggctttcaaa tcggatatca
      781 gacgtccaat taaatagtcg agttgaatga tgaaagcggt tgtaacatca tccatcgctt
      841 tcattttcgc aaccggcagg gtattcaccc cctcacgccg ccgcgatccg aagcgtttgc
      901 cctgattcag agccaattcc acggccagcg gaatgttcag aatggcggca tccgtcgaac
      961 ctaatgtttt tcccggcgcg gtgggttcga cgcagccgat ggccacataa tcgcgtgcgt
     1021 cgcggatatc ataaccggcg gaggtcatgg ccggtatgat gacatcatca ttgaagagag
     1081 caggtgtatt gctgccctcg gccagcgccg tgtatatcgc ctttttaaat tcgggtggcg
     1141 atccgctgtg aatacgcgcg tgaaaattcg gctgtctcat ccgcaattcc gtggcaattt
     1201 ccagaagcag gtaactcaat tcatttgtgg catcgttgcc ttcctgatcc tggcctccca
     1261 tggtcaccac ctgccagctc ggcatgccgc cggcaaggtt ggtaatcgcc tgagaataaa
     1321 cgggaatggt ttcgcacaac ttaatgcaaa aagattcgag cagctctttt gcgcgcttgc
     1381 gggtgatcag atgggcctcc cggtctttct tataatacgg ataaagaatc tgatcgatgc
     1441 ggccgggaca aacggtttcc cccatgcttt cctgaaaaat agccacatgc agaaagaaaa
     1501 gactctgaag cgcttcatgg aacgtccgcg ccccccctcg cggaacgtga tggcataccg
     1561 cggcgatatc atgaagctcc aggcttctca tggcatcctc ttctttttcg gcaagaacct
     1621 cggcgcaggc tgcgtaacgc tcaccgaaaa gcgccagcgc atccgccgat aggagtgccg
     1681 catgataaaa cgattcctgg tccggatttt tattttttag ctttaaagct tctatttcct
     1741 ttttgatttc atccgttcct aaacggatca gcttttcatg atcgggagcc agatgagcga
     1801 taccgcccgc ctcgtaaata taataa
//
LOCUS       DAONQZ010000171         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_67520_length_1792_cov_21.819229, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000171 DAONQZ010000000
VERSION     DAONQZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67520_length_1792_cov_21.819229"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLR13_12355"
     CDS             <1..837
                     /locus_tag="PLR13_12355"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HQC20177.1"
                     /translation="VRHDAKLEHNYFLMENLGKMLFEFVQNLVAKGRSSFCFEDVAKL
                     KDSSPEAIRAALRRLMKKGDIAMPYRGFYVIVPPEYRIAGCLPPEQFIPDLMEHVSEV
                     YYTGLLSAAEYYGAAHQRPQVFQVVVARSRRPIACGKMRVDFVFRKNAAIIPKEKINT
                     PAGELQLSSPEATALDLVGYVHHCAGLDNVATVLAELAEKIDAEKLRNLADLSPIAWV
                     QRLGYLLELVDAKDKAQGIAGYVKEKHPVRAPLVPALAHTGAKMDSRWRVFINTKVEP
                     DL"
     gene            834..1634
                     /locus_tag="PLR13_12360"
     CDS             834..1634
                     /locus_tag="PLR13_12360"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQC20178.1"
                     /translation="MISAAYIKEWRRAAPWQKNDQVEQDLIISRALVEIFNQSVLAEN
                     LAFRGGTALYKLHVQPVRYSEDIDLVQVHPGPIGSLMDALKEVLNPWLGNPKRKQSEG
                     RITLTYRMTAESGFPLKLKVEINSREHFSILGLEKRTLDIQSRWFSGSAAILTYQLDE
                     LLGTKMRALYQRKKGRDLFDLWKALNTADAQPRRVIDCFLRYMKHEGHKVSQAEFEQN
                     LIGKLDDPTFVADVGPLLITDTAFDFETAARDVINKMLPLMPGEPWQG"
BASE COUNT          420 a          428 c          517 g          427 t
ORIGIN      
        1 gtccgccatg atgcaaaact tgaacataat tattttctaa tggaaaatct gggtaaaatg
       61 ctgtttgagt ttgttcaaaa tctggtcgcg aaaggtcgct cctctttctg ctttgaggat
      121 gtggcaaagc tcaaggattc ttccccggaa gccatccggg cagcgctgcg ccgtttgatg
      181 aaaaaaggcg atattgccat gccttacagg ggattttatg tcattgttcc gccggagtac
      241 cgaatcgccg gttgcctgcc tccggagcaa ttcattcccg acctgatgga acatgtcagc
      301 gaggtttatt ataccggcct cttgagcgcc gccgagtatt acggcgcggc gcatcaacgg
      361 ccgcaggttt ttcaggttgt cgttgccaga agcagaaggc cgattgcctg cggcaagatg
      421 cgggtggatt ttgtctttcg gaaaaacgcg gccataatcc caaaggaaaa gatcaatact
      481 ccggcgggcg agttgcagct ttcctcgccg gaagcgacgg cgcttgacct tgtgggttat
      541 gttcatcatt gcgcggggct ggacaatgtg gccacggttc tggctgaact ggcggaaaaa
      601 atcgatgcgg aaaagttacg aaacctggcc gatttatccc cgattgcctg ggtgcagcgg
      661 ctgggttatc tgcttgagct ggtggacgca aaagacaagg cgcaagggat tgccgggtat
      721 gtgaaagaaa aacacccggt gcgcgcaccg cttgtgcctg cgctggcgca taccggagca
      781 aaaatggaca gtcgctggcg tgtttttatc aataccaagg tggagccgga tttatgattt
      841 cggctgctta catcaaggag tggcggcggg cggcgccctg gcaaaaaaac gatcaggtgg
      901 aacaggattt gattatcagc cgcgctctgg ttgaaatatt taaccagagc gttttggcgg
      961 aaaatcttgc tttccgtggc gggacggctc tttataaatt gcatgtgcag ccggtgcgct
     1021 attcggagga tattgatctg gtgcaggtgc atcctggacc catcggatcg ctgatggatg
     1081 cgttaaaaga ggttctcaat ccctggctgg gcaatccgaa gcgcaagcag tcggaaggcc
     1141 ggatcacatt gacttaccgc atgacggcgg aaagtggttt cccgcttaaa cttaaagtgg
     1201 aaatcaattc gcgtgagcat ttctcgatac ttgggttgga aaaaagaact ctggatattc
     1261 aatcccgctg gttttccggc agcgccgcga ttttgaccta tcagctcgat gagctcctgg
     1321 ggacgaaaat gcgggctttg tatcagcgca aaaagggtcg ggaccttttt gatttatgga
     1381 aggcgctcaa cactgcagac gcccaacccc gtcgcgtgat cgattgcttc ctcagataca
     1441 tgaaacacga aggccataag gtatcgcagg cggagtttga acaaaacctg atcgggaaac
     1501 tggatgatcc gacgtttgtg gccgacgtgg gaccattgtt aattacggac accgcttttg
     1561 atttcgagac ggcggcccgg gatgtgataa ataagatgct tcccctcatg ccgggcgagc
     1621 cctggcaagg ctaagaccta tttttcaacc cgacgggccg gaccgctttc atcgctcggt
     1681 cattcagaag gcaagagatg cggctaatgg ctgaccgtcc ggcgagcctt ttccaggcat
     1741 tcgatgaggt cctgcatgtg ccgctcttca ataaaaggcg acatgacaaa tg
//
LOCUS       DAONQZ010000172         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_67865_length_1786_cov_10.099365, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000172 DAONQZ010000000
VERSION     DAONQZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67865_length_1786_cov_10.099365"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLR13_12365"
     CDS             <1..600
                     /locus_tag="PLR13_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20179.1"
                     /translation="QFCQTDVASCRVSAFSRSEWPNFASEEGKVERRRPDVLTDRFPC
                     ISIAAVSSSGRVHKTALPCGEDRFPFVNGMDQPIQISRQPRMVRNEKMIADESLTVKD
                     KKFPAMPFIGRIQYLSNRHCRKHLFVHRCHQAQTIAGNPGGDPLSPKEDSIDFVKRSS
                     SVRKPLLVQRKTRQFVFSVFTKSLAFFPPMVRSILRSCS"
     gene            complement(583..>1786)
                     /locus_tag="PLR13_12370"
     CDS             complement(583..>1786)
                     /locus_tag="PLR13_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HQC20180.1"
                     /translation="AKEVQFEGNLRFLDRDAAAGDKIPGIFLVRSLEGELYIVSIPSD
                     DELKRTGAEAYYANLKEMLGNKLVFRALLTEGVLDGQPYTFIRFLEPPAQLLLDKIFK
                     ISIVFMFFFVMLGMGLNLTLKDFKLVLQKPKGIAIGIIFQWLLMPLLALGMAWALGFY
                     HKFPFIYAGMVLICACPGGVTSNLMTYYAKGDLALSVSLTSISTFLALFFTPLILTVL
                     CANIPDITIPTGLIAQTIIGLVIVPLIIGMSIRQKWLAFAVKATPFFSALGVIALLMV
                     IGVGVYTNVEKFADTQRYSLIFYIMVVSLSLLGMLLAAFFPKLFRIDNYQTRAISIEV
                     GLRNSTLAVTIALLIQDLMGDFYSSMFVTTAIYGITMYITGFLMILLYKKILPLDTDS
                     NTSSRTGS"
BASE COUNT          516 a          422 c          447 g          401 t
ORIGIN      
        1 caattctgtc aaacagacgt tgcatcatgt cgagtaagcg ctttctctcg ctccgagtgg
       61 ccgaattttg ccagtgaaga ggggaaagta gagaggaggc gccctgatgt attgacggat
      121 cgcttcccct gcatttcaat tgctgctgtt tcttccagtg gccgtgtcca taagacggct
      181 cttccctgcg gcgaagatag attccctttt gtgaacggga tggaccaacc aatccaaata
      241 agcaggcagc cccggatggt aagaaacgaa aaaatgatag cagatgaaag cttaacggta
      301 aaggataaaa aattccctgc catgccgttt attgggcgca tccaatattt gtcaaatagg
      361 cattgccgta agcatctttt tgttcacaga tgccaccagg cacagaccat tgccggcaat
      421 ccgggtggcg atcccctgtc gccaaaagaa gattcaattg atttcgtcaa acgttcttca
      481 tccgtcagga aacccctgct tgtccaaagg aaaacgcggc aattcgtgtt cagcgtcttc
      541 acaaaaagct tggcgttttt ccctccgatg gttagatcaa ttttacgatc ctgttcgtga
      601 agacgtgttc gaatccgtgt ccaggggcag aattttctta tacagaagaa tcatcagaaa
      661 ccctgtgata tacatcgtaa tgccgtaaat ggccgtggtt acgaacatgg agctgtaaaa
      721 gtcacccatg aggtcctgga tcaacagggc aatggtcacc gccagcgttg aattgcgcag
      781 cccaacttcg atggagatgg ctctggtctg ataattatca atgcggaaca actttgggaa
      841 aaacgccgcc agcagcatac ccagaaggga aagagaaact accatgatgt agaatatcag
      901 actgtagcgc tgggtgtcgg cgaatttttc aacgtttgtg taaacgccta ctccgatgac
      961 catcaggagc gcgatgactc caagggctga aaagaaaggt gtggccttta ccgcgaaagc
     1021 aagccatttc tgccggatgg acatcccgat gatgagaggc acgatgacca gaccgatgat
     1081 ggtctgtgca atcaggcccg tcggtatggt gatatcagga atattcgcgc acaacacggt
     1141 aagaatcaga ggagtgaaaa aaagcgccag aaaagttgaa atggaggtca gggagaccga
     1201 aagagccaga tctcccttcg cgtagtaggt cataagattt gaagtgacgc cgcccggaca
     1261 agcgcagatc aaaaccatcc cggcataaat aaatggaaat ttgtggtaaa atcccagggc
     1321 ccaggccatt ccaagagcca agagcggcat gagaagccat tgaaaaataa ttccgatggc
     1381 aatgcccttt ggcttctgca ggacaagctt gaaatccttg agcgtaaggt taagccccat
     1441 tccgagcata acgaaaaaga acataaaaac aatggaaatc ttaaaaatct tgtccagcag
     1501 tagctgggcc ggcggctcaa ggaaccggat gaaggtatag ggttgaccgt ctaaaacgcc
     1561 ttcggtcagc agagcccgaa aaaccagttt gtttccgagc atttctttaa gattagcata
     1621 ataggcttca gcgccggtcc tttttaattc atcatcggaa gggatcgaga cgatatagag
     1681 ttcgccttcg aggctcctca ccaaaaaaat accggggatc ttgtccccgg cagcggcatc
     1741 cctgtcaaga aaacggaggt tcccttcaaa ctgaacctcc ttggcc
//
LOCUS       DAONQZ010000173         1772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_68627_length_1772_cov_13.845661, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000173 DAONQZ010000000
VERSION     DAONQZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68627_length_1772_cov_13.845661"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>1772
                     /locus_tag="PLR13_12375"
     CDS             146..>1772
                     /locus_tag="PLR13_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQC20181.1"
                     /translation="MIKIQNAQVLHQIQEDLKRSYDPNQKIITICAGTGCRGYGCIKV
                     KDALEAELSKHNLNVEIRATGCFGFCEKGPLVVIYPEKIFYQQVKVNDVKDIVEKTVA
                     KGEILEKLLYRDPNTKETVLKEEDVPFYKKQLRLVFGSNGIIDPTRIESYITIGGYSA
                     LAKALTEMNPEGIINEIKKASLRGRGGGGFQAGTKWEGCRKAHGSPKYIIANGDEGDP
                     GAYMDRSLMEGNPHSIVEGMIIGAYAIGASHGYIYVRNEYPLAVIHLTMAIESAREAG
                     LLGKDILGSGFDFDISINKGGGAFVCGESSALFASIEGRAGEPRAKYVHAVEKGLWDK
                     PTVLNNVETWANVPLIINNGADWYASIGTEKSKGTKIFSLVGKINNTGLVEVPMGMTL
                     REIIYDIGGGIPNGRKFKAVQTGGPSGGCIPESLLDLPVDFDKLYEVGSMMGSGGMIV
                     MDDRSCMVDVAKYFLSFLQEESCGKCVPCREGIRRMREILEDICAGKGQEGDIELLEK
                     IATGVADGSLCALGSSAPNPVLSTLRYFRDEYDA"
BASE COUNT          458 a          414 c          499 g          401 t
ORIGIN      
        1 ataaattctc tctggaaacc gtcaactgcg tgggcgcctg cgcgttgggg cctgtcgtag
       61 ttatcgatac cgaatatcac ggccagatga ccatgaataa agtggataag gttatcggca
      121 agttcaaagg agagggggag taatcatgat aaaaatacaa aacgcacagg tcttgcacca
      181 gattcaggaa gatctgaaac gaagttacga ccccaatcag aaaataatca ccatttgtgc
      241 cgggaccgga tgccgtggtt atggttgcat caaggtcaag gatgcactgg aggcggaact
      301 ttccaaacat aatttaaacg tggaaattcg agcgactggc tgtttcggtt tttgtgaaaa
      361 aggtcctctg gttgtcattt acccggaaaa aatattttat caacaggtca aggtgaatga
      421 tgtaaaagat attgttgaaa agaccgtggc aaagggtgaa attttagaaa aactcctgta
      481 ccgcgatcca aatacaaagg agacggtgct caaggaagaa gatgttccct tctacaaaaa
      541 gcagttgcgc ctggttttcg gatccaacgg cattatcgat cctacccgaa tcgaatccta
      601 tatcaccatt ggtggttaca gtgcgctggc caaagcctta acggaaatga atcccgaagg
      661 catcatcaat gaaatcaaaa aagcatcgct tcgcggacgc ggcggcggcg gatttcaggc
      721 cggaacgaaa tgggagggtt gccgcaaggc tcacggctcg cccaaatata tcattgccaa
      781 cggcgacgaa ggagatcccg gcgcgtatat ggatcgctcg ctgatggaag gcaatccgca
      841 cagtatcgtt gagggcatga tcatcggcgc ttacgcgata ggcgcttctc acgggtatat
      901 ttatgtgcgc aacgaatatc cgctggcggt cattcattta accatggcca tcgaaagcgc
      961 ccgggaagcc ggactgctgg ggaaagatat tctgggcagc ggatttgatt ttgatatttc
     1021 gattaacaag ggcggcggtg cttttgtctg cggcgaatcg tccgcgcttt ttgcgtccat
     1081 tgaaggacgc gccggtgagc cgcgcgccaa gtatgtccac gccgtggaaa aagggctgtg
     1141 ggacaaaccc accgttctca ataatgtcga aacctgggcc aacgttcctt tgattatcaa
     1201 taacggcgcc gactggtacg cttccatcgg cacagaaaag agcaagggca ccaagatctt
     1261 ctcgctggtg ggcaagatca ataacacggg actcgtggaa gttccgatgg gcatgacgct
     1321 gcgcgagatc atttatgaca ttggcggcgg tattcccaac ggcaggaagt tcaaagccgt
     1381 tcagacgggc ggaccttcag gtggctgcat tcccgaaagc ctgctggatt tgccggtgga
     1441 tttcgataag ctctatgaag tcggttccat gatgggatcc ggcggcatga tcgtcatgga
     1501 tgaccgttcc tgcatggtgg acgtggccaa atattttctc tcctttttgc aggaagaatc
     1561 ctgcggcaag tgtgttccct gccgtgaagg tatccgccgc atgcgggaaa ttctcgaaga
     1621 catctgcgcg ggcaagggtc aagaaggcga catcgagctt ctggaaaaaa tagcaaccgg
     1681 cgtggcggac ggttctttgt gcgcgctggg tagctcggcg cccaatccgg tgctcagcac
     1741 gcttcgatat ttccgcgatg agtacgacgc gc
//
LOCUS       DAONQZ010000174         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_69590_length_1755_cov_32.329412, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000174 DAONQZ010000000
VERSION     DAONQZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69590_length_1755_cov_32.329412"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1021)
                     /locus_tag="PLR13_12380"
     CDS             complement(59..1021)
                     /locus_tag="PLR13_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20182.1"
                     /translation="MMKKLNDKRYLVFSGLAVGVSLLLVAAFNYVMDPYNLLGNNRTG
                     VYFWNERQVKDVILTYKHEGILIGSSKTGYVNPDDLSCYRFYNASMRGMVPEEMYFYL
                     KKYLRREKLVLVGFDFFMFNEREFPLIRIADWDDLRYGRMEYLLSAETVKSSWKTLTK
                     WRKKEKKHGMKANGQFDYPGASEAPASPGSQQQYEKQYQDIIKGLVQNHYYKFSFSQT
                     RIDYVKKIKTLLEENKIPYAVFINPLNHDVLAALQNIDAYLLFLNWKREMKNIFPDIY
                     DYSISRYSVREGFYREDPFHYTNDTGIAFLNEIISDFCPEGSKP"
     gene            complement(1022..>1755)
                     /locus_tag="PLR13_12385"
     CDS             complement(1022..>1755)
                     /locus_tag="PLR13_12385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQC20183.1"
                     /translation="NVIDFWRRWHMSLSRFLRDYLYIPLGGNRKGEAMRFVNIMITML
                     LGGLWHGAAWTFVLWGGLHGLFLIINHGWVKIKRRFGYEGKGGRTGRFFSGALTFLAV
                     TVAWVFFRADSAATAFRIIGSMAGQNGFALPEKYLDKFSVLAMPLQAWGVDFHSMHYF
                     KGSNEVVYILIALLVVWLAPNVQQIMDLCMPYLNIMAKGKKLAQPPAWLTWRPSYFWA
                     IVLVVCALVSLLSMGRVSEFLYFQF"
BASE COUNT          501 a          445 c          373 g          436 t
ORIGIN      
        1 caccatcaga aaagagcctt ccgattcaaa tctgatttac cggttcagga aaaagatttt
       61 acggctttga cccttccgga caaaaatcac tgataatctc atttaaaaac gctattcctg
      121 tgtcattagt gtaatgaaag ggatcctccc gataaaatcc ctcccgcact gaataccggc
      181 tgatggagta atcatagata tcaggaaaaa tattcttcat ttcccgcttc cagttcaaga
      241 acagcaagta agcgtcaata ttttgaagcg ccgccagcac gtcgtgattc agaggattga
      301 tgaataccgc atagggaatt ttattttctt ccagaagcgt tttgatcttt ttcacataat
      361 cgatccgtgt ttgcgaaaaa gaaaacttgt aataatggtt ttgtaccagc cctttgatga
      421 tatcctgata ttgcttttca tattgctgct gagatcccgg agaagcgggg gcctccgatg
      481 ctcccggata gtcgaattgc ccgttcgcct tcatgccgtg ctttttttct tttttgcgcc
      541 acttggtcaa ggtcttccat gacgatttaa cggtttccgc gcttagcagg tattccatcc
      601 tgccatagcg cagatcatcc cagtccgcga ttctgatcag cggaaattct ctttcattga
      661 acatgaagaa atcaaagccc accaacacca gtttttcacg gcgcaggtat ttctttaaat
      721 aaaaatacat ctcttccgga accattccgc gcatggaagc gttgtaaaat cgatagcagg
      781 aaaggtcgtc gggattgaca tatccggttt tgctggaccc aatgagaatt ccttcatgtt
      841 tataggtcaa tatcacgtct ttgacctgac gctcgttcca aaagtaaacg cccgtcctgt
      901 tattgcccag cagattgtaa gggtccataa cataattgaa tgctgcaacc agcagcaacg
      961 agaccccgac ggccaggccc gaaaaaacaa gatatctttt atcgtttaat ttcttcatca
     1021 tttaaaattg aaaatagaga aattcgctga cccggcccat ggaaagcaat gaaaccagag
     1081 cgcacacgac aagcacaatc gcccagaagt atgagggacg ccaggtcagc catgccggcg
     1141 gttgggccag tttcttgccc ttggccataa tatttaaata cggcatgcaa agatccatga
     1201 tttgctggac attgggcgcc agccagacaa ccagcagggc aatgaggata taaaccactt
     1261 cattgctgcc cttaaagtaa tgcatggaat gaaaatcaac cccccaggcc tgaagcggca
     1321 tggccagcac agaaaattta tccagatatt tttccggcag ggcaaaaccg ttttgaccgg
     1381 ccatggaacc gatgatgcga aacgcagtgg cggcgctgtc ggccctgaaa aaaacccaag
     1441 ccaccgtaac ggcaagaaat gtaagcgctc cggagaagaa acggccggtc cgtccgccct
     1501 ttccttcata accaaaccgc cttttgattt ttacccatcc gtgattgatg atcagaaata
     1561 aaccgtgcaa cccgccccac agaacaaagg tccatgccgc gccgtgccaa agccctccca
     1621 gcagcatggt gatcattata ttgacaaagc gcatggcttc tcccttgcgg ttgccgccca
     1681 gaggaatata gagataatcc cgcaaaaaac gggaaagcga catatgccat cttctccaaa
     1741 aatcgatgac gttta
//
LOCUS       DAONQZ010000175         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_71024_length_1729_cov_17.132616, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000175 DAONQZ010000000
VERSION     DAONQZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71024_length_1729_cov_17.132616"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..627)
                     /locus_tag="PLR13_12390"
     CDS             complement(52..627)
                     /locus_tag="PLR13_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQC20184.1"
                     /translation="MDSKKKTIIILLVIVALLTVSAYSFFAGNYNTFVRLDQAVKSAW
                     AQVENQLQRRYDLIPNLVETVKGYAKHEREVLTEVTNARAKVGGAGTVPDKIAANNEL
                     SGALSRLLLVVERYPDLKANQNFLHLQDELAGTENRIAVERMRYNEAVKVYNQTIKSF
                     PANLLAGMFGFREAAFFDAPKEAKTAPKVQF"
     gene            complement(661..1386)
                     /locus_tag="PLR13_12395"
     CDS             complement(661..1386)
                     /locus_tag="PLR13_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20185.1"
                     /translation="MSKIPDKPQDIFIDLADDYKKVFGSDLVSLIIYGSAAAGFYVKG
                     KSDINLLVVLTQNGINRLEDAMDTVKKWMKRRVSVPWVMTGAFINNSLDCYPIEFLNL
                     KINHVLIHGEDVLTPLQFKPEDLRLQIEKELKGKLVLLRQGYLETEGKSKHIKELIGR
                     SLMAFISIFNALIYLKKGSAPPRRRETVKELAGIYAIDDDVFLKCIDIKEGKDKLSGD
                     EIVVLFKKYISEVEKIIHIVDAL"
BASE COUNT          422 a          460 c          369 g          478 t
ORIGIN      
        1 taattcgacc atcaaatttc agtgcactcc gtttctgaaa tttgggtctc attaaaactg
       61 aaccttgggc gctgttttgg cttctttcgg cgcatcaaag aacgcagcct cccggaaacc
      121 gaacatgccg gcaagcagat tggccggaaa tgatttgatg gtctgattgt aaactttgac
      181 cgcttcgttg taccgcattc tctccacggc aattctgttt tccgtacccg ccagttcatc
      241 ctgcaggtgt aagaagttct gattcgcttt caggtcggga tacctttcaa caaccagaag
      301 caagcggctt aatgcgccgg acagctcgtt attggccgct attttatcgg gaaccgtacc
      361 ggcaccgccg actttggcgc gggcgttggt cacttcggtg agaacctctc tttcgtgttt
      421 ggcgtacccc ttgaccgttt cgaccagatt gggaatcaaa tcatagcgcc gctgcaattg
      481 attttccacc tgcgcccagg cgctcttgac ggcctgatcg agcctgacaa atgtattgta
      541 attgccggcg aaaaaagagt aagcacttac ggtcagaaga gcaactataa ccaaaaggat
      601 aatgatcgtt ttctttttgg aatccatttt ttacctcccc tatcgtgtta aattgataaa
      661 ttataacgca tcaacgatgt gaatgatttt ttcgacttcg ctgatgtact ttttgaataa
      721 taccacaatt tcatctccgg agagcttatc ctttccttcc ttaatatcga tgcatttcag
      781 aaacacatca tcatcgatgg cataaatccc ggccagctct tttaccgttt cgcgccgtct
      841 gggcggcgcc gatcccttct tcagataaat cagcgcatta aaaatggaga taaaggccat
      901 cagggaacga ccgatgagct ctttgatgtg tttggacttc ccttccgtct ccagataacc
      961 ctggcgcagc aaaaccagct ttcccttgag ttccttctcg atttgcagac gcagatcctc
     1021 cggtttgaat tgcagcggcg tcagaacgtc ctcaccgtgg atcaaaacat ggttgatctt
     1081 cagattcaga aattcgatcg gatagcaatc cagagaattg ttgatgaacg ccccggtcat
     1141 tacccagggc acggacacgc gtctcttcat ccattttttg accgtatcca tggcgtcttc
     1201 cagtctgttg atgccgtttt gagttaagac aaccagcaga ttgatgtccg actttccttt
     1261 gacataaaat ccggcggcag cgcttccgta aatgatgagc gaaacaagat cgctgccgaa
     1321 cactttttta taatcatctg ccaaatcaat gaaaatatcc tgtggtttgt ccggtatttt
     1381 tgacatttta ttcacccttt atgttttaac gaatcccaaa ttatcccgca atgcggcggg
     1441 aatgaacaac cccaccatcc aaaggaactg cgccccaagg ggcggggaat taaacccaca
     1501 tttatcccgc tcggcctata gtgaccttta ggttatccac ctcggggata attcgactta
     1561 caaatatagt cccacatggc ttcgcatgtg ggataattcg actaaccaat ctgactgcgc
     1621 ttcgcttgtc atatcggagt ctcattaaaa tttgggtctc attaaaaact ccggccggcg
     1681 ccgccgccgc cgctcatgcc gcccccgaac cctccgaaac cgccgccga
//
LOCUS       DAONQZ010000176         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_71685_length_1718_cov_10.339146, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000176 DAONQZ010000000
VERSION     DAONQZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71685_length_1718_cov_10.339146"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..648)
                     /gene="hisH"
                     /locus_tag="PLR13_12400"
     CDS             complement(22..648)
                     /gene="hisH"
                     /locus_tag="PLR13_12400"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQC20186.1"
                     /translation="MIAIIEYNAGNITSVARALHSIGQDFIITNDTKMLDAASCAIFP
                     GVGAAGAAMAYLKEKKLDVWLKNCFRSGKPILGICLGTQIILDASEENDTTCIGLVSG
                     TTRRFPENLAADGRALKIPHMGWNSVAFRRPHPVFDSVSAQAEFYFVHSYYPSPADPS
                     AVLGTTEYGMTFCSALAVNNLVAVQFHPEKSGRPGLQILKNFCAWEGK"
     gene            complement(838..1362)
                     /locus_tag="PLR13_12405"
     CDS             complement(838..1362)
                     /locus_tag="PLR13_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20187.1"
                     /translation="MMNKNKLTMMMFGMWALMAAISVFVAVSANAEEWKIKDIGVGLI
                     VEGEQYDKQLIIVERSNVWDAKELYENGAQAGKYHGNQLFAAWIQGPPTKEYQNRYGT
                     PVWMYKYKIIYPDGKTFESGPHAFYTPGHAYFGIKTGGYTNGSWKIDWYIQHRDTNEI
                     RHVGTSEFTTTYGR"
BASE COUNT          396 a          460 c          375 g          487 t
ORIGIN      
        1 agggaatgat tcttttggag atcattttcc ctcccatgcg cagaaattct tcagaatctg
       61 caggcccggc cggccgcttt tttccgggtg aaactgcacg gccaccagat tgttcaccgc
      121 cagcgccgag cagaacgtca tgccgtattc ggtcgtaccc agcacagccg acggatcggc
      181 aggcgacggg taataggaat gaacgaagta aaattccgcc tgggccgata cgctgtcaaa
      241 aaccggatgc ggacgccgga aggccacgct gttccagccc atgtggggga ttttcagggc
      301 ccggccatcc gcggccaggt tttccggaaa acgccgggtg gtcccgctga ccaggccgat
      361 gcacgtcgtg tcgttttctt cggaagcgtc cagaatgatc tgcgtgccca gacagatgcc
      421 cagaatcggc tttccggatc gaaaacaatt tttgagccac acatccagct ttttttcctt
      481 gaggtaagcc atcgccgcgc ccgccgcgcc gacccccggg aaaatagcac aggacgcggc
      541 atcgagcatt tttgtatcat ttgtaatgat aaaatcctgg ccgatggaat gcagcgccct
      601 cgccacgctg gtaatgtttc cggcattata ttcgataatt gcaatcatct tactcaaatt
      661 tcctgaaaaa tcatcctgca cgctccaaat ttactagcaa atttaatacc caatcgccaa
      721 gatcaatttc agttgttcct gcgctaaact ttttcaccac gtctgttttt tcgtcccggc
      781 cggtaataat ttaatggtcg ctgtgcctat ttccccactt gccgtttgag gaccagttta
      841 tctcccatag gtagtcgtaa attcactggt cccgacatgt ctaatctcat tcgtatcacg
      901 gtgctgtata taccagtcta ttttccagga accgtttgtg tagccgcctg ttttgatgcc
      961 gaaataagca tgccccggcg tgtaaaaagc gtgtgggccg gattcaaagg tttttccgtc
     1021 ggggtagatg attttgtatt tgtacatcca taccggcgtg ccgtaacggt tctgatactc
     1081 tttggtaggg ggaccttgta tccaggcggc aaacaactga ttgccatgat atttgccggc
     1141 ctgcgcgcca ttttcataaa gttctttggc atcccaaaca ttgcttcgct ctacaattat
     1201 caattgttta tcgtactgtt ccccttcaac aatgagacct acgccaatgt ctttaatttt
     1261 ccattcttcc gcatttgctg aaactgcaac aaaaaccgaa attgcagcca tcaatgccca
     1321 cataccgaac atcatcatgg tcagtttatt cttgttcatc atctattgag ctcctctctt
     1381 accgtttcca catatttacg tatcattgca tcccgatcgc tcaggtccgt catccgtcaa
     1441 tctgattcga atatatctga ttcagcatac gaagcgcttc ccggttttct tcaacttcat
     1501 tcgctgtatt attggcatca acaaaaaatc acctggagca caacgatcgg gtggattgac
     1561 ttggttgtac tgttattgct ttggaaaata tttgaccttc ccgatatttt taaagtccac
     1621 atcctgcgtc catatcgtcg ctttgaattc ctgggccgtt gcgagaataa tactgtctgc
     1681 catgggaagc tgatgctcca gactcaactt tgccgctg
//
LOCUS       DAONQZ010000177         1693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_73227_length_1693_cov_12.061661, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000177 DAONQZ010000000
VERSION     DAONQZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73227_length_1693_cov_12.061661"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..503)
                     /locus_tag="PLR13_12410"
     CDS             complement(138..503)
                     /locus_tag="PLR13_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20188.1"
                     /translation="MQEITFITYKGVKILYEDLENAKSEEVVPWIERAKAVIRSQPEK
                     SVLALLNVKNAKFDTEVTTALKEFAKGNDPYIKFAAVYGIEGLKEIIFKSVLTFTGRK
                     NLVLCKTLEEAKDILASKA"
     gene            712..1659
                     /locus_tag="PLR13_12415"
     CDS             712..1659
                     /locus_tag="PLR13_12415"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HQC20189.1"
                     /translation="MEHIRVGISSCLLGNNVRYDGGHKLDRFLTDTLGKYVDYVPVCP
                     EVECGLSIPRPSMRLEGHPDAPRLIVTSTREDLTERMKAWAKKRVAELEKEDLCGFIF
                     KSDSPSSGMERVKVYNEKSMPVKNGVGLFAAMFMKHFPLLPVEDEGRLHDPGLRENFI
                     ERIFALKKWRETLSAKNFKKSLIDFHTKHKLLILAHSTKHYQMMGKLVAAQKNFPAKD
                     IQQQFQDLLLEALKMKATPAKHVNVLQHMMGYFKEQLSADEKKELLELIHAYRDGYIP
                     LIVPVTLISHYVRKYDEPYLKDQVYLNPHPSELQLRNHV"
BASE COUNT          480 a          382 c          358 g          473 t
ORIGIN      
        1 cccctgtgct ttgcacaggg gaccattcac ttaggtttta gtgaaccttg attttctgat
       61 gcgcagtgat cgaaaaatct tataatgaat tatcctgcca atttctggcg acagggacaa
      121 aggtcattgt ccctgtttta agctttgctc gccagaatgt cttttgcttc ttcaagtgtt
      181 ttacaaagaa caaggttctt cctgccggtg aaggtcagaa cgcttttgaa tattatctcc
      241 ttcaaacctt ctataccgta aacggcagcg aatttgatat aggggtcgtt tcccttcgca
      301 aattccttca gggcggttgt tacctctgtg tcaaattttg catttttgac attgagcagc
      361 gcaagcacag atttttcggg ctgacttcga ataaccgctt tagccctttc tatccatggt
      421 accacttcct cggacttagc attttcaaga tcttcatata gtatttttac gcccttataa
      481 gtgataaaag ttatttcttg catatcccca cctttttata atggaatttt gtttaccttc
      541 aaaggttagc gtataatcac ttcacgtcgc cagtcttatt cttggctcat tatcgcttat
      601 tgataaatca tactcacatc gaaaaataaa tcaatatttt gtaccgctgt tgtatttccg
      661 ctttatctga tacaatattt tgaaaagtca taattatgac gggagaaaaa aatggaacac
      721 attagggtcg gtatcagttc ctgtttgctg ggcaataacg tgcgttatga cggcggacac
      781 aagctggaca gatttttaac cgacacgctg gggaaatatg tggactatgt tccggtttgt
      841 ccggaagtcg aatgcggcct gagtattccc agaccttcca tgcgcctgga aggccatccc
      901 gacgcgccga ggttaatcgt taccagcacc agagaggatc tgaccgaacg catgaaagcc
      961 tgggcgaaaa aacgcgtcgc tgagctggaa aaagaagacc tctgcggttt catttttaaa
     1021 agcgattccc ccagcagcgg catggaacgg gtcaaggttt acaatgaaaa atccatgccg
     1081 gttaaaaacg gcgtaggact gtttgctgca atgttcatga aacattttcc gctgctgccg
     1141 gtggaagacg aaggccggtt acacgatccc ggcctgcgcg aaaactttat tgaaagaata
     1201 ttcgcgctta aaaaatggcg ggaaacgtta agcgcgaaaa acttcaaaaa gagtctgatc
     1261 gattttcaca ccaaacataa actattaatc ctcgcgcaca gcaccaaaca ttatcaaatg
     1321 atgggcaaac tggtggcagc acaaaaaaac ttcccggcaa aagatatcca acaacagttc
     1381 caggatctgc tgttggaggc tttgaaaatg aaagccacgc cggccaaaca cgtcaatgtc
     1441 ctgcagcata tgatgggcta ttttaaggaa caattgtccg ccgatgaaaa aaaagaattg
     1501 ctggaattga ttcatgctta ccgcgacgga tatattcccc tgattgtgcc ggtaacgctg
     1561 atcagccact atgtgcgcaa atacgacgag ccttatctca aagatcaggt atacctgaac
     1621 ccacatcctt cggaactgca gttgagaaac cacgtgtaag ttgtcaaaat ttcaacttga
     1681 catccattta ttc
//
LOCUS       DAONQZ010000178         1612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_78401_length_1612_cov_16.662171, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000178 DAONQZ010000000
VERSION     DAONQZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78401_length_1612_cov_16.662171"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1225
                     /locus_tag="PLR13_12420"
     CDS             155..1225
                     /locus_tag="PLR13_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20190.1"
                     /translation="MRRTVVVVTLNYQNQPGKVNAERGEVSAMETTIKGYLEGLTLGE
                     IQIHKHVAVIPVMGTNGSGPDYLTMKEAMDRRLIQIGEVGESGHVPELKVINTGLKPV
                     LLLDGEELAGAKQNRVLNTTVLIREKSETIIPVSCTEHGRWSYRSQYFEESCHIMSAR
                     LRGVKNASVHENLKDRSAFASDQFAVWDGIRVQATANKVDAPTGAMRDVLKAKQEDLD
                     DFLEHFPLMDRQKGLLVLVNGEVVGMDVISRKEAFALLYSKLVKSYVMDALTEMPKKN
                     KDAGTMKAADFLKKIPVCGESAFDSVGYGRDYRYEGEGLVGSALVHEGTVIHLAFFQI
                     NKADKTGRMSSVNRRRSYRTNQ"
     gene            1242..>1612
                     /locus_tag="PLR13_12425"
     CDS             1242..>1612
                     /locus_tag="PLR13_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20191.1"
                     /translation="MSLMDRYEALKKEIVDRKKPFDRFITILEGETTWLTSPASTRFH
                     LAEEQGLLKHSVGVAETLLRFREFLAPAIPEESCVIVGLFHDAGKLGMPGNPLYLPND
                     DEWMVKNRGICYKTNPDVVAMG"
BASE COUNT          428 a          388 c          472 g          324 t
ORIGIN      
        1 ttctgggcgg ggatccagca ccttgtcatt cccgcatttt tctggcggga atccaggaat
       61 aattgaatca tggatgcccg acaaaaaact tcgggcatga caaaacattt tgcaatcagc
      121 tccatgaaga gaaaataaat ccaaaagtaa aataatgcga cgtaccgttg tcgttgtgac
      181 attgaactat cagaaccaac ccggtaaagt caatgccgaa agaggggagg tatcggccat
      241 ggaaacaacg atcaaagggt atctggaagg attgacactt ggtgagattc agatccacaa
      301 acatgtcgcc gtcattccgg tcatggggac aaacggttcc ggaccggact atctgaccat
      361 gaaggaggcg atggaccgca ggctgattca gatcggggaa gtgggagaga gcggccatgt
      421 cccggagctg aaggtcatca acaccggcct gaagcctgtt ttgctcctgg acggggaaga
      481 gcttgccggg gccaagcaga accgtgtcct caacaccacc gtcctgatca gggaaaaatc
      541 ggaaaccatc attccggtaa gctgcaccga gcacggtcgc tggtcctacc ggtcgcagta
      601 cttcgaggaa tcctgccaca tcatgtcggc cagactgcgc ggcgtcaaga acgcttccgt
      661 gcatgagaac ctgaaagaca ggagcgcttt tgcatccgat cagttcgccg tatgggacgg
      721 aatcagggtt caggcaacgg ccaacaaagt cgatgctccg accggcgcca tgagagacgt
      781 actcaaagcg aagcaggaag acctggacga ttttctggag cattttccgc tgatggacag
      841 gcaaaaaggc ctgctggtcc tggtgaacgg agaggttgtc ggcatggacg tgatctcaag
      901 gaaagaggcc ttcgcgttgc tgtattcaaa actggtcaaa agctacgtca tggacgcgct
      961 gacggaaatg ccgaagaaaa acaaagacgc cggcacgatg aaagcggcag acttcctgaa
     1021 aaagattccg gtctgcgggg aaagcgcctt tgactccgtg ggctacgggc gcgattaccg
     1081 atacgaagga gagggtctgg tcggctccgc gctggtccat gagggcacgg tgatccatct
     1141 ggccttcttt cagatcaaca aagcggataa gacaggccgc atgtcctctg tcaaccgcag
     1201 acgatcctac cggacgaacc agtaagcgaa gaggtaaggt tatgagtttg atggatcgtt
     1261 atgaagcatt gaaaaaggag attgtggacc gaaagaaacc gtttgaccgc tttattacca
     1321 ttctggaggg tgagaccacc tggctcacct ctccggcaag cacaaggttt catctggcag
     1381 aagaacaggg tctcttgaag cacagcgtcg gggtagcgga aactctcctg cgtttccggg
     1441 aatttcttgc cccggcgatt cctgaggaga gctgtgtcat cgtcggcctg tttcatgatg
     1501 cgggaaaact gggaatgccc ggcaatcccc tgtatttgcc gaatgatgat gagtggatgg
     1561 tgaaaaacag ggggatatgc tacaagacga acccggacgt tgttgcgatg gg
//
LOCUS       DAONQZ010000179         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_81595_length_1568_cov_14.257766, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000179 DAONQZ010000000
VERSION     DAONQZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81595_length_1568_cov_14.257766"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLR13_12430"
     CDS             complement(<1..403)
                     /locus_tag="PLR13_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQC20192.1"
                     /translation="MGIIYFDNAATSWPKPPEVSAAMQKFMKKIGANPGRSGHRLSVE
                     AARIIFNTREKLAQLFNIDDPLRIVMTKNATEGLNIAVSGLLKSGDHVITSSMEHNSV
                     MRPLRAKLHRGVELTVIPCDRKGLIDPAHVNG"
     gene            complement(549..>1568)
                     /locus_tag="PLR13_12435"
     CDS             complement(549..>1568)
                     /locus_tag="PLR13_12435"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HQC20193.1"
                     /translation="YYLLEYALLIMVIIFFSWIIILCYKHYMSMDRFKNITMQQMEAI
                     MALVEEGSFSRAAKKMLLTQPALTKNIKNAEDCLGARLANRSSAGISLTPEGKILYDY
                     ARRMIRLRDEAKGKILALSKESGGNIYLSASTIPATYILPRALSDFNKTNSDIHIYIR
                     TADSEEAMNIVMDNEVEMGVIGKHPLNKKLKADALWKDRLVLVVSRNHAWCKKKTIAL
                     AELLNEPFVIREKGSATRELFESCLRKTKATSHVPSNICGELGSSEAVKEAVIAGWGV
                     SVISIHAVSRELSQGLLCEIPVQGLTMERNFYLIYRRQFDFRPFHQAFIKFIKRYTPA
                     FPDEN"
BASE COUNT          420 a          377 c          328 g          443 t
ORIGIN      
        1 ctccgtttac gtgcgccgga tcaatcaagc ctttccgatc gcagggaatg acagttagtt
       61 cgacacctct gtgcaacttc gcccgcagtg ggcgcatcac cgaattgtgc tccatgctcg
      121 acgtgataac atgatctcct gatttcagca ggccggagac cgcgatattc aatccttccg
      181 tggcattttt cgtcatcacg atccgcagag gatcgtctat gttgaagagt tgcgcaagtt
      241 tttcacgggt attgaaaata atgcgcgcag cctccaccga caggcgatgc ccggaacggc
      301 ctggattggc cccgatcttc ttcatgaatt tttgcatggc cgcactgact tccggcggct
      361 tcggccacga cgtggcggca ttatcaaaat aaataattcc catattattt ccagtccaat
      421 cagcaaaaca cctgcttaat tcggttaaaa attatatgct ttctgaaaat atatccaata
      481 aataaaagtt ataaaaaaca aaacagtatt tctttttttt attgcaacat tcgggcagcg
      541 gacgatgatt aattttcatc cggaaatgca ggtgtgtagc gtttaatgaa tttgataaac
      601 gcttgatgga aagggcggaa gtcaaactga cgtcggtaga ttaaatagaa atttctttcc
      661 atggtgagtc cctgaaccgg aatttcacaa agcaatccct gggagagttc gcgcgatacg
      721 gcatgaatgg aaatcaccga tacgccccat ccggcaataa ccgcttcttt gacggcttcg
      781 gaacttccca gttcaccgca gatattggat ggaacatggc tggttgcttt tgttttcctt
      841 aaacaggatt caaataattc tctcgttgcc gaaccttttt ccctgatgac aaacggttcg
      901 ttcaatagtt ccgctaaagc aattgtcttc tttttgcacc aggcatgatt tcgggaaacg
      961 acaagaacga ggcgatcttt ccacagggcg tctgccttta gctttttatt cagaggatgt
     1021 ttgccgatga cgcccatttc cacctcatta tccataacta tgttcatggc ctcttcgcta
     1081 tcggcggttc tgatataaat atgaatatcg gaatttgttt tattaaaatc actcaaggct
     1141 cgaggcagaa tatacgtggc gggaatcgtg ctggcgctca aataaatatt cccgccggat
     1201 tctttactca atgcaagaat cttgcccttc gcttcatccc gaagcctaat catacgacgc
     1261 gcatagtcgt ataaaatctt gccttccggc gtcaacgaga ttcccgcgct gctgcgattg
     1321 gccaatcgcg cacccagaca atcttccgca ttcttgatat ttttggtcag cgccggctgg
     1381 gtcaaaagca ttttcttggc agcgcggctg aaactgccct cctccactaa ggccataatg
     1441 gcttccatct gctgcatggt aatatttttg aaacgatcca tagacatgta atgcttatag
     1501 cacaatataa tgatccatga aaagaaaatt ataaccatta ttaataaagc atattccaaa
     1561 agataata
//
LOCUS       DAONQZ010000180         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_85604_length_1517_cov_10.306430, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000180 DAONQZ010000000
VERSION     DAONQZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85604_length_1517_cov_10.306430"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLR13_12440"
     CDS             <1..255
                     /locus_tag="PLR13_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQC20194.1"
                     /translation="IGKLSDLEDEWIQRYFDHLSKGTLAEGAKLKIERMPIKMQCNSC
                     SQTYEVEIAGLENLVCPHCGAEGASLLSGREYYIKDMEVQ"
     gene            255..929
                     /gene="hypB"
                     /locus_tag="PLR13_12445"
     CDS             255..929
                     /gene="hypB"
                     /locus_tag="PLR13_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQC20195.1"
                     /translation="MAEVRLIEVKEEILADNIKVAAGVRENLQKTKTMLVNLMSSPGS
                     GKTSLILQTIAGLKDSVKLGVIEADIDSMVDAEKVAAKGIRAVQLKTGGFCHLDATMV
                     TQGIDALSVGDLDLVIIENVGNLVCPAEFDTGAHKNVMILSVPEGDDKPLKYPLMFSI
                     CDVLLVNKIDYLSLSDFDMEAMRKRVLALNPKIEIIEVSCKTGAGIDQWVGWLKKEVA
                     AFKNLK"
     gene            985..>1517
                     /locus_tag="PLR13_12450"
     CDS             985..>1517
                     /locus_tag="PLR13_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQC20196.1"
                     /translation="MTIDEKYKKAGEIISTAGGTPLPVNDTLIKLLKFFIMEDELDFI
                     AAFAEKKSQTMEQLKQSSGLSEDEINKKVKDLAKRGVIFNQPNSKGIMVFRLLPLFNV
                     GTFEYNFMGKLVIDDRNKEIAGLFKQMFADLNNMVQDNYDVIVPMLMAGPAIDRTVPV
                     FENKPKSEPVKIVINEKL"
BASE COUNT          446 a          305 c          416 g          350 t
ORIGIN      
        1 atcggcaaac tcagcgatct ggaagatgaa tggattcagc gctattttga tcatttgagc
       61 aagggcacgc tggccgaagg ggccaaactc aaaatcgagc ggatgccgat taaaatgcag
      121 tgtaattcct gttcccaaac ctatgaggtc gagatagcgg ggttggaaaa tcttgtatgt
      181 ccccattgcg gagcagaggg cgcttcgctt ctctcgggac gggaatatta tattaaggat
      241 atggaggtcc agtaatggca gaagtaagat tgattgaagt caaggaggaa atcctggcgg
      301 ataatatcaa agtggcggcg ggagtccggg agaatctgca aaagacaaaa accatgctgg
      361 tcaatctgat gtcctcgccg gggtcgggca agaccagcct gattctgcaa accattgccg
      421 gtttgaaaga cagcgtgaaa ctgggcgtga tagaggcgga tatcgattcg atggtggacg
      481 cggaaaaagt cgccgccaag ggaatccgcg ccgtgcagct caagacggga gggttctgtc
      541 atctggatgc cacgatggtt acgcagggca ttgacgcttt aagcgtgggc gatctggatc
      601 ttgttattat cgaaaacgtg ggcaatctgg tctgcccggc ggaattcgat accggcgccc
      661 ataaaaatgt gatgattctg agtgtgcccg aaggggacga caaaccgctt aaatacccgc
      721 tgatgttttc catctgtgat gtgctgctgg tcaataaaat tgattatttg tcgctgagcg
      781 attttgatat ggaagccatg cgcaaacggg ttctggcgtt gaatcccaaa attgagatca
      841 tcgaggtttc ctgcaaaacg ggagcgggaa tcgatcaatg ggtcggctgg ctgaagaaag
      901 aagttgcagc gtttaaaaat ttgaaataga ttgcctatca ttaacgggac aaacattcac
      961 aaaaaacaag agagggcaga aaacatgacg attgatgaaa aatataaaaa agccggagaa
     1021 attatcagca cagcgggagg tacgccgctt cccgtcaacg atacgctgat caaacttctg
     1081 aagtttttta tcatggaaga cgaactggat tttattgccg cttttgcgga aaagaaatcc
     1141 cagaccatgg aacagttaaa gcagtccagc ggcctgtccg aagacgagat caataaaaaa
     1201 gtgaaagacc tggcgaaaag gggcgttatt ttcaatcagc ccaacagcaa aggcatcatg
     1261 gttttccgtt tgctgccgct gtttaacgtt ggcacgttcg aatacaattt catgggtaaa
     1321 ctggtcattg acgacaggaa taaggaaatt gccggattgt tcaagcagat gtttgcggat
     1381 ttgaacaata tggttcagga caattacgat gtgatcgtcc cgatgctgat ggccggaccg
     1441 gccatcgaca gaacggtgcc tgtctttgaa aataaaccca agagtgaacc ggttaaaatc
     1501 gtcatcaatg aaaaact
//
LOCUS       DAONQZ010000181         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_4317
            NODE_86664_length_1504_cov_18.407177, whole genome shotgun
            sequence.
ACCESSION   DAONQZ010000181 DAONQZ010000000
VERSION     DAONQZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866288
            Sequence Read Archive: SRR5007352
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 03:49:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,469
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86664_length_1504_cov_18.407177"
                     /isolate="AMR_MDS_4317"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="miaB"
                     /locus_tag="PLR13_12455"
     CDS             <1..945
                     /gene="miaB"
                     /locus_tag="PLR13_12455"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQC20197.1"
                     /translation="FHPSLSSMSIFVPPRDGQISAFVTIMQGCNNFCAYCVVPYLRGR
                     EISRTSADVLDEIGRLADAGVMEVTLLGQNVNSYGKTLHDGSNFASLIKKIGDIEGIK
                     RIRFTTSHPRDLSGEMIDCFRTEEKLCAHIHLPVQSGSNHVLALMNRGYTVEDYMQKV
                     DALRRTRPDISITSDMIVGFPGETEKDYQETIDMMEKIRFDSTFSFKYSEREGTAAAK
                     LKDSVPEGEKLRRLAALQDLQNRHTLEKNTQLLGTRQQVLVEGRSKNTANDLTGRTGS
                     WKIVNFAGPSELKGQTVVVGITEAYVHSLRGRIEKTEQ"
     gene            958..1458
                     /locus_tag="PLR13_12460"
     CDS             958..1458
                     /locus_tag="PLR13_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQC20198.1"
                     /translation="MLVEMKVLGLTIDPVTNTPIVILKDMDNNKAIPIWIGLFEASAI
                     ATEMKKIAFPRPLTHDLFVEFLRKFDIAVNRIEIVDIRNNTFFANIYLMKEGLDYAID
                     ARPSDAIAIALRTNAPIFVDETVIEKSRNVDFGVKLGDLDKMKDDKLKEFLENLSMED
                     FGKYKM"
BASE COUNT          417 a          331 c          412 g          344 t
ORIGIN      
        1 tttcatccgt cgttgagctc gatgagcatt tttgttccgc cccgggacgg ccagatcagc
       61 gcctttgtga cgattatgca gggatgcaat aatttctgcg cgtactgcgt tgtcccttat
      121 ctgcgcggac gggagatcag ccggacgtcc gccgacgtcc tcgatgagat cggcagactt
      181 gccgatgcgg gggtcatgga agtgacgctg ctcggacaaa atgtgaactc ttacggcaaa
      241 acgctgcatg acggatcgaa ttttgcgtcg ctgattaaaa agataggcga catcgaaggc
      301 atcaagcgga tacggttcac gacgtcgcat ccgcgcgatc tgtccggcga aatgatcgat
      361 tgtttcagga cggaagaaaa actctgcgca cacattcatc ttccggttca atccggttcc
      421 aatcacgttc tggcgctgat gaaccgggga tatacggttg aggattatat gcagaaagtg
      481 gatgctttgc gcaggaccag gcccgacatc agcattacct cggatatgat cgtcggattt
      541 ccgggagaaa ccgaaaaaga ttaccaagaa acaattgaca tgatggaaaa aatcaggttt
      601 gattcaactt tctctttcaa gtattctgaa agggagggga cagcggccgc aaaattaaaa
      661 gactcggttc cggaaggcga aaagttaaga cgcctggcgg cgctgcagga tttgcagaat
      721 cggcacacgc tggaaaaaaa tacgcaactg ctgggaacgc ggcagcaggt gctggtggaa
      781 ggccgaagca aaaataccgc caatgatctg acgggacgaa cgggatcatg gaaaattgtc
      841 aattttgcgg gcccttcaga gttgaaaggc caaacggttg tcgtcggcat aacggaagcg
      901 tatgtgcatt ctttaagagg ccggatagaa aaaacagaac aataggagag attgtccgtg
      961 ttagttgaaa tgaaggttct gggtttgacc atcgatcccg taaccaatac gccgattgtg
     1021 atattaaagg atatggataa caacaaggcc atcccgatct ggatcggttt gtttgaggcc
     1081 agcgcgattg ccacggaaat gaaaaagatt gcgtttcctc gaccgctgac ccacgatctg
     1141 tttgtcgagt ttttgcgcaa gttcgatatt gccgtaaacc ggattgaaat tgtcgatatc
     1201 cgcaacaaca cttttttcgc caatatttat ttgatgaaag aggggctgga ttatgccatt
     1261 gacgcacgtc ccagcgatgc gatcgccatc gccctgagga ccaacgcccc gatattcgtg
     1321 gatgagacgg tcatcgaaaa atcgcgcaat gtcgatttcg gcgtcaagct gggcgatctg
     1381 gataaaatga aagatgataa actgaaagaa tttctggaaa atctttccat ggaggatttc
     1441 ggcaaatata agatgtaatg aggctcgcta aagacgagcg cagcgaagtc tgaagcgcaa
     1501 gccg
//
